@article{15251, abstract = {Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.}, author = {Ying, Wei and Wang, Yaowei and Wei, Hong and Luo, Yongming and Ma, Qian and Zhu, Heyuan and Janssens, Hilde and Vukašinović, Nemanja and Kvasnica, Miroslav and Winne, Johan M. and Gao, Yongxiang and Tan, Shutang and Friml, Jiří and Liu, Xin and Russinova, Eugenia and Sun, Linfeng}, issn = {1095-9203}, journal = {Science}, number = {6689}, pages = {eadj4591}, publisher = {American Association for the Advancement of Science}, title = {{Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export}}, doi = {10.1126/science.adj4591}, volume = {383}, year = {2024}, } @article{15252, abstract = {A measurable map between measure spaces is shown to have bounded compression if and only if its image via the measure-algebra functor is Lipschitz-continuous w.r.t. the measure-algebra distances. This provides a natural interpretation of maps of bounded compression/deformation by means of the measure-algebra functor and corrobo-rates the assertion that maps of bounded deformation are a natural class of morphisms for the category of complete and separable metric measure spaces.}, author = {Dello Schiavo, Lorenzo}, issn = {1848-8013}, journal = {Mathematical Communications}, number = {1}, pages = {137--142}, publisher = {Udruga Matematicara Osijek}, title = {{A characterization of maps of bounded compression}}, volume = {29}, year = {2024}, } @inproceedings{15253, abstract = {We study the problem of maintaining a differentially private decaying sum under continual observation. We give a unifying framework and an efficient algorithm for this problem for any sufficiently smooth function. Our algorithm is the first differentially private algorithm that does not have a multiplicative error for polynomially decaying weights. Our algorithm improves on all prior works on differentially private decaying sums under continual observation and recovers exactly the additive error for the special case of continual counting from Henzinger et al. (SODA 2023) as a corollary. Our algorithm is a variant of the matrix mechanism whose error depends on the γ2 and γF norm of the underlying matrix. We give a constructive proof for an almost exact upper bound on the γ2 and γF norm and an almost tight lower bound on the γ2 norm for a large class of lower-triangular matrices. This is the first non-trivial lower bound for lower-triangular matrices whose non-zero entries are not all the same. It includes matrices for all continual decaying sums problems, resulting in an upper bound on the additive error of any differentially private decaying sums algorithm under continual observation. We also explore some implications of our result in discrepancy theory and operator algebra. Given the importance of the γ2 norm in computer science and the extensive work in mathematics, we believe our result will have further applications.}, author = {Henzinger, Monika H and Upadhyay, Jalaj and Upadhyay, Sarvagya}, booktitle = {Proceedings of the 2024 Annual ACM-SIAM Symposium on Discrete Algorithms}, location = {Alexandria, VA, United States}, pages = {995--1018}, publisher = {Society for Industrial and Applied Mathematics}, title = {{A unifying framework for differentially private sums under continual observation}}, doi = {10.1137/1.9781611977912.38}, volume = {2024}, year = {2024}, } @article{15257, abstract = {Root gravitropic bending represents a fundamental aspect of terrestrial plant physiology. Gravity is perceived by sedimentation of starch-rich plastids (statoliths) to the bottom of the central root cap cells. Following gravity perception, intercellular auxin transport is redirected downwards leading to an asymmetric auxin accumulation at the lower root side causing inhibition of cell expansion, ultimately resulting in downwards bending. How gravity-induced statoliths repositioning is translated into asymmetric auxin distribution remains unclear despite PIN auxin efflux carriers and the Negative Gravitropic Response of roots (NGR) proteins polarize along statolith sedimentation, thus providing a plausible mechanism for auxin flow redirection. In this study, using a functional NGR1-GFP construct, we visualized the NGR1 localization on the statolith surface and plasma membrane (PM) domains in close proximity to the statoliths, correlating with their movements. We determined that NGR1 binding to these PM domains is indispensable for NGR1 functionality and relies on cysteine acylation and adjacent polybasic regions as well as on lipid and sterol PM composition. Detailed timing of the early events following graviperception suggested that both NGR1 repolarization and initial auxin asymmetry precede the visible PIN3 polarization. This discrepancy motivated us to unveil a rapid, NGR-dependent translocation of PIN-activating AGCVIII kinase D6PK towards lower PMs of gravity-perceiving cells, thus providing an attractive model for rapid redirection of auxin fluxes following gravistimulation.}, author = {Kulich, Ivan and Schmid, Julia and Teplova, Anastasiia and Qi, Linlin and Friml, Jiří}, issn = {2050-084X}, journal = {eLife}, keywords = {General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine, General Neuroscience}, publisher = {eLife Sciences Publications}, title = {{Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism}}, doi = {10.7554/elife.91523}, volume = {12}, year = {2024}, } @article{15258, abstract = {Inclusion at academic events is facing increased scrutiny as the communities these events serve raise their expectations for who can practically attend. Active efforts in recent years to bring more diversity to academic events have brought progress and created momentum. However, we must reflect on these efforts and determine which underrepresented groups are being disadvantaged. Inclusion at academic events is important to ensure diversity of discourse and opinion, to help build networks, and to avoid academic siloing. All of these contribute to the development of a robust and resilient academic field. We have developed these Ten Simple Rules both to amplify the voices that have been speaking out and to celebrate the progress of many Equity, Diversity, and Inclusivity practices that continue to drive the organisation of academic events. The Rules aim to raise awareness as well as provide actionable suggestions and tools to support these initiatives further. This aims to support academic organisations such as the Deep Learning Indaba, Neuromatch Academy, the IBRO-Simons Computational Neuroscience Imbizo, Biodiversity Information Standards (TDWG), Arabs in Neuroscience, FAIRPoints, and OLS (formerly Open Life Science). This article is a call to action for organisers to reevaluate the impact and reach of their inclusive practices.}, author = {Hall, Siobhan Mackenzie and Kochin, Daniel and Carne, Carmel and Herterich, Patricia and Lewers, Kristen Lenay and Abdelhack, Mohamed and Ramasubramanian, Arun and Michael Alphonse, Juno Felecia and Ung, Visotheary and El-Gebali, Sara and Currin, Christopher and Plomp, Esther and Thompson, Rachel and Sharan, Malvika}, issn = {1553-7358}, journal = {PLOS Computational Biology}, number = {3}, publisher = {Public Library of Science}, title = {{Ten simple rules for pushing boundaries of inclusion at academic events}}, doi = {10.1371/journal.pcbi.1011797}, volume = {20}, year = {2024}, } @article{15295, abstract = {Background: Benzodiazepines and antidepressants are effective agents for the treatment of generalized anxiety disorder (GAD), with the HAM-A frequently used as a primary outcome measure. The GAD literature is inconsistent regarding which medications are more effective for somatic versus psychic symptoms of GAD, and treatment guidelines do not advocate for prescribing based on subtype. This meta-analysis aimed to determine whether benzodiazepines and antidepressants have a differential impact on the somatic versus psychic subscales of the HAM-A in GAD. Methods: An electronic search was undertaken for randomized controlled trials of either benzodiazepines or antidepressants for GAD that reported treatment response using the HAM-A subscales. Data were extracted by independent reviewers. A random effects assessment of weighted mean difference with 95% confidence intervals and subgroup difference was applied. All analysis was done on SPSS 26. An assessment of bias, and of quality of evidence was performed. Results: 24 randomized controlled trials met the inclusion criteria: 18 antidepressant trials, 5 benzodiazepine trials and 1 of both. 14 studies were assessed as having between some and high risk of bias, while 10 were assessed as having low risk of bias. Benzodiazepines (WMD of 1.81 [CI 1.03, 2.58]) were significantly more effective than antidepressants (WMD of 0.83 [CI 0.64, 1.02]) for reducing somatic symptoms of GAD (Chi2 = 5.81, p = 0.02), and were also more effective (WMD of 2.46 [CI 1.83, 3.09]) in reducing psychic symptoms than antidepressants (WMD of 1.83 [CI 1.55, 2.10]), although this comparison did not reach statistical significance (Chi2 = 3.31, p = 0.07). Conclusion: The finding that benzodiazepines were significantly more effective than antidepressants for somatic symptoms needs to be weighed up against potential benefits of antidepressants over benzodiazepines. It may be useful for future treatment guidelines for GAD to explicitly consider symptom subtype.}, author = {Beyer, Chad and Currin, Christopher and Williams, Taryn and Stein, Dan J.}, issn = {1532-8384}, journal = {Comprehensive Psychiatry}, publisher = {Elsevier}, title = {{Meta-analysis of the comparative efficacy of benzodiazepines and antidepressants for psychic versus somatic symptoms of generalized anxiety disorder}}, doi = {10.1016/j.comppsych.2024.152479}, volume = {132}, year = {2024}, } @inproceedings{15296, abstract = {In this paper we build a constructive algorithm that returns a rectifiable curve that connects two points in a weakly convex set in a Hilbert space. We have proven that this algorithm converges and obtained an estimate on the curve’s length and compare the length of the curve obtained to known results.}, author = {Lopushanski, Mariana and Ivanov, Grigory}, booktitle = {AIP Conference Proceedings}, issn = {1551-7616}, location = {Virtual}, number = {1}, publisher = {AIP Publishing}, title = {{A constructive algorithm for building rectifiable curves in weakly convex sets}}, doi = {10.1063/5.0195908}, volume = {3030}, year = {2024}, } @article{15301, abstract = {Plant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predictable tissue deformation after wounding. This deformation causes instant redirection of expansion in the surrounding cells and reorientation of microtubule arrays, ultimately predicting cell division orientation. Microtubules are destabilized under low tension, whereas stretching of cells, either through wounding or external aspiration, immediately induces their polymerization. The higher microtubule abundance in the stretched cell parts leads to the reorientation of microtubule arrays and, ultimately, informs cell division planes. This provides a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical forces for tissue reconstruction and plant architecture.}, author = {Hörmayer, Lukas and Montesinos López, Juan C and Trozzi, N and Spona, Leonhard and Yoshida, Saiko and Marhavá, Petra and Caballero Mancebo, Silvia and Benková, Eva and Heisenberg, Carl-Philipp J and Dagdas, Y and Majda, M and Friml, Jiří}, issn = {1878-1551}, journal = {Developmental Cell}, number = {10}, pages = {1333--1344.e4}, publisher = {Elsevier}, title = {{Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization}}, doi = {10.1016/j.devcel.2024.03.009}, volume = {59}, year = {2024}, } @article{15313, abstract = {Our goal is to investigate fundamental properties of the system of internally cooled convection. The system consists of an upward thermal flux at the lower boundary, a mean temperature lapse-rate and a constant cooling term in the bulk with the bulk cooling in thermal equilibrium with the input heat flux. This simple model represents idealised dry convection in the atmospheric boundary layer, where the cooling mimics the radiative cooling to space notably through longwave radiation. We perform linear stability analysis of the model for different values of the mean stratification to derive the critical forcing above which the fluid is convectively unstable to small perturbations. The dynamic behavior of the fluid system is described and the scaling of various important measured quantities such as the total vertical convective heat flux and the upward mass flux is measured. We introduce a lapse-rate dependent dimensionless Rayleigh-number Ray that determines the behavior of the system, finding that the convective heat-flux and mass-flux scale approximately as Ray0.5 and Ray0.7 respectively. The area-fraction of the domain that is occupied by upward and downward moving fluid and the skewness of the vertical velocity are studied to understand the asymmetry inherent in the system. We conclude with a short discussion on the relevance to atmospheric convection and the scope for further investigations of atmospheric convection using similar simplified approaches.}, author = {Agasthya, Lokahith N and Muller, Caroline J}, issn = {1007-5704}, journal = {Communications in Nonlinear Science and Numerical Simulation}, publisher = {Elsevier}, title = {{Dynamics and scaling of internally cooled convection}}, doi = {10.1016/j.cnsns.2024.108011}, volume = {134}, year = {2024}, } @article{15314, abstract = {Development of electroactive materials exhibiting high performance with superior stability is crucial in the field of supercapacitors and battery research. We report on synthesis of highly stable composite of semi-polycrystalline polyaniline and graphene (SPani-graphene) and its application in supercapacitor electrodes. The electrochemical behavior and device performance of the electrodes were investigated through cyclic voltammetry (CV), galvanostatic charge-discharge GCD) and electrochemical impedance spectroscopy (EIS) in a 3-electrode and a 2-electrode (device) cell configurations, repectively. The cell specific capacitance (Cell Csp) achieved from the 2-electrode symmetric cell configuration was 525.5 F g−1 at 0.1 A g−1 using polymer gel electrolyte (PGE). The PGE in this work is xanthan gum jellied in 1 M aq. Na2SO4. The maximum energy density and power density achieved from the device was 46.7 Wh kg−1 and 16.16 kW kg−1, respectively, at 0.4 A g−1. Furthermore, the device exhibits an excellent retention of cell specific capacitance and coulombic efficiency of 97 % and 94 %, respectively, over 10,000 continuous GCD cycles, indicating an excellent rate capability as well as a promising power management. To investigate the material's electrochemical durability, a detailed EIS study has been carried out using both, 3-electrode, and 2-electrode cell configurations, before and after long cycling test (over 10,000 continuous GCD cycles). Our thorough experimentation delivers satisfactory results and has been explained in detail in the manuscript. Hereby, we propose that the EIS technique can be adopted for investigating materials' electrochemical stability, in addition to long CV and GCD cycles in the field of supercapacitors and battery research.}, author = {Mahato, Neelima and Singh, Saurabh and Sreekanth, T. V.M. and Yoo, Kisoo and Kim, Jonghoon}, issn = {2352-152X}, journal = {Journal of Energy Storage}, publisher = {Elsevier}, title = {{Polycrystalline phases engineered in-situ in polyaniline-graphene composite evoluting an exceptional stability and high charge storage capacity: An EIS investigative approach to evaluate material's stability}}, doi = {10.1016/j.est.2024.111464}, volume = {88}, year = {2024}, } @article{15315, abstract = {Single and collective cell migration are fundamental processes critical for physiological phenomena ranging from embryonic development and immune response to wound healing and cancer metastasis. To understand cell migration from a physical perspective, a broad variety of models for the underlying physical mechanisms that govern cell motility have been developed. A key challenge in the development of such models is how to connect them to experimental observations, which often exhibit complex stochastic behaviours. In this review, we discuss recent advances in data-driven theoretical approaches that directly connect with experimental data to infer dynamical models of stochastic cell migration. Leveraging advances in nanofabrication, image analysis, and tracking technology, experimental studies now provide unprecedented large datasets on cellular dynamics. In parallel, theoretical efforts have been directed towards integrating such datasets into physical models from the single cell to the tissue scale with the aim of conceptualising the emergent behaviour of cells. We first review how this inference problem has been addressed in both freely migrating and confined cells. Next, we discuss why these dynamics typically take the form of underdamped stochastic equations of motion, and how such equations can be inferred from data. We then review applications of data-driven inference and machine learning approaches to heterogeneity in cell behaviour, subcellular degrees of freedom, and to the collective dynamics of multicellular systems. Across these applications, we emphasise how data-driven methods can be integrated with physical active matter models of migrating cells, and help reveal how underlying molecular mechanisms control cell behaviour. Together, these data-driven approaches are a promising avenue for building physical models of cell migration directly from experimental data, and for providing conceptual links between different length-scales of description.}, author = {Brückner, David and Broedersz, Chase P.}, issn = {1361-6633}, journal = {Reports on Progress in Physics}, number = {5}, publisher = {IOP Publishing}, title = {{Learning dynamical models of single and collective cell migration: a review}}, doi = {10.1088/1361-6633/ad36d2}, volume = {87}, year = {2024}, } @article{15316, abstract = {Genomic DNA that resides in the nuclei of mammalian neurons can be as old as the organism itself. The life span of nuclear RNAs, which are critical for proper chromatin architecture and transcription regulation, has not been determined in adult tissues. In this work, we identified and characterized nuclear RNAs that do not turn over for at least 2 years in a subset of postnatally born cells in the mouse brain. These long-lived RNAs were stably retained in nuclei in a neural cell type–specific manner and were required for the maintenance of heterochromatin. Thus, the life span of neural cells may depend on both the molecular longevity of DNA for the storage of genetic information and also the extreme stability of RNA for the functional organization of chromatin.}, author = {Zocher, Sara and Mccloskey, Asako and Karasinsky, Anne and Schulte, Roberta and Friedrich, Ulrike and Lesche, Mathias and Rund, Nicole and Gage, Fred H. and Hetzer, Martin W and Toda, Tomohisa}, issn = {1095-9203}, journal = {Science}, number = {6691}, pages = {53--59}, publisher = {AAAS}, title = {{Lifelong persistence of nuclear RNAs in the mouse brain}}, doi = {10.1126/science.adf3481}, volume = {384}, year = {2024}, } @article{15317, abstract = {We consider the open symmetric exclusion (SEP) and inclusion (SIP) processes on a bounded Lipschitz domain Ω, with both fast and slow boundary. For the random walks on Ω dual to SEP/SIP we establish: a functional-CLT-type convergence to the Brownian motion on Ω with either Neumann (slow boundary), Dirichlet (fast boundary), or Robin (at criticality) boundary conditions; the discrete-to-continuum convergence of the corresponding harmonic profiles. As a consequence, we rigorously derive the hydrodynamic and hydrostatic limits for SEP/SIP on Ω, and analyze their stationary nonequilibrium fluctuations. All scaling limit results for SEP/SIP concern finite-dimensional distribution convergence only, as our duality techniques do not require to establish tightness for the fields associated to the particle systems.}, author = {Dello Schiavo, Lorenzo and Portinale, Lorenzo and Sau, Federico}, issn = {1050-5164}, journal = {Annals of Applied Probability}, number = {2}, pages = {1789--1845}, publisher = {Institute of Mathematical Statistics}, title = {{Scaling limits of random walks, harmonic profiles, and stationary nonequilibrium states in Lipschitz domains}}, doi = {10.1214/23-AAP2007}, volume = {34}, year = {2024}, } @article{15319, abstract = {Exocyst component of 70-kDa (EXO70) proteins are constituents of the exocyst complex implicated in vesicle tethering during exocytosis. MILDEW RESISTANCE LOCUS O (MLO) proteins are plant-specific calcium channels and some MLO isoforms enable fungal powdery mildew pathogenesis. We here detected an unexpected phenotypic overlap of Arabidopsis thaliana exo70H4 and mlo2 mlo6 mlo12 triple mutant plants regarding the biogenesis of leaf trichome secondary cell walls. Biochemical and Fourier transform infrared spectroscopic analyses corroborated deficiencies in the composition of trichome cell walls in these mutants. Transgenic lines expressing fluorophore-tagged EXO70H4 and MLO exhibited extensive colocalization of these proteins. Furthermore, mCherry-EXO70H4 mislocalized in trichomes of the mlo triple mutant and, vice versa, MLO6-GFP mislocalized in trichomes of the exo70H4 mutant. Expression of GFP-marked PMR4 callose synthase, a known cargo of EXO70H4-dependent exocytosis, revealed reduced cell wall delivery of GFP-PMR4 in trichomes of mlo triple mutant plants. In vivo protein–protein interaction assays in plant and yeast cells uncovered isoform-preferential interactions between EXO70.2 subfamily members and MLO proteins. Finally, exo70H4 and mlo6 mutants, when combined, showed synergistically enhanced resistance to powdery mildew attack. Taken together, our data point to an isoform-specific interplay of EXO70 and MLO proteins in the modulation of trichome cell wall biogenesis and powdery mildew susceptibility.}, author = {Huebbers, Jan W. and Caldarescu, George A. and Kubátová, Zdeňka and Sabol, Peter and Levecque, Sophie C.J. and Kuhn, Hannah and Kulich, Ivan and Reinstädler, Anja and Büttgen, Kim and Manga-Robles, Alba and Mélida, Hugo and Pauly, Markus and Panstruga, Ralph and Žárský, Viktor}, issn = {1532-298X}, journal = {Plant Cell}, number = {4}, pages = {1007--1035}, publisher = {Oxford University Press}, title = {{Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew}}, doi = {10.1093/plcell/koad319}, volume = {36}, year = {2024}, } @article{15320, abstract = {Josephson diodes are superconducting elements that show an asymmetry in the critical current depending on the direction of the current. Here, we theoretically explore how an alternating current bias can tune the response of such a diode. We show that for slow driving there is always a regime where the system can only carry zero-voltage dc current in one direction, thus effectively behaving as an ideal Josephson diode. Under fast driving, the diode efficiency is also tunable, although the ideal regime cannot be reached in this case. We also investigate the residual dissipation due to the time-dependent current bias and show that it remains small. All our conclusions are solely based on the critical current asymmetry of the junction, and are thus compatible with any Josephson diode.}, author = {Seoane Souto, Rubén and Leijnse, Martin and Schrade, Constantin and Valentini, Marco and Katsaros, Georgios and Danon, Jeroen}, issn = {2643-1564}, journal = {Physical Review Research}, number = {2}, publisher = {American Physical Society}, title = {{Tuning the Josephson diode response with an ac current}}, doi = {10.1103/PhysRevResearch.6.L022002}, volume = {6}, year = {2024}, } @inproceedings{15321, abstract = {Boolean Networks (BNs) are widely used as a modeling formalism in several domains, notably systems biology and computer science. A fundamental problem in BN analysis is the enumeration of trap spaces, which are hypercubes in the state space that cannot be escaped once entered. Several methods have been proposed for enumerating trap spaces, however they often suffer from scalability and efficiency issues, particularly for large and complex models. To our knowledge, the most efficient and recent methods for the trap space enumeration all rely on Answer Set Programming (ASP), which has been widely applied to the analysis of BNs. Motivated by these considerations, our work proposes a new method for enumerating trap spaces in BNs using ASP. We evaluate the method on a mix of 250+ real-world and 400+ randomly generated BNs, showing that it enables analysis of models beyond the capabilities of existing tools (namely pyboolnet, mpbn, trappist, and trapmvn).}, author = {Trinh, Giang and Benhamou, Belaid and Pastva, Samuel and Soliman, Sylvain}, booktitle = {Proceedings of the 38th AAAI Conference on Artificial Intelligence}, isbn = {1577358872}, issn = {2374-3468}, number = {9}, pages = {10714--10722}, publisher = {Association for the Advancement of Artificial Intelligence}, title = {{Scalable enumeration of trap spaces in boolean networks via answer set programming}}, doi = {10.1609/aaai.v38i9.28943}, volume = {38}, year = {2024}, } @article{15330, abstract = {Clathrin-mediated endocytosis (CME) is vital for the regulation of plant growth and development by controlling plasma membrane protein composition and cargo uptake. CME relies on the precise recruitment of regulators for vesicle maturation and release. Homologues of components of mammalian vesicle scission are strong candidates to be part of the scission machinery in plants, but the precise roles of these proteins in this process are not fully understood. Here, we characterised the roles of Plant Dynamin-Related Proteins 2 (DRP2s) and SH3-domain containing protein 2 (SH3P2), the plant homologue to Dynamins’ recruiters, like Endophilin and Amphiphysin, in the CME by combining high-resolution imaging of endocytic events in vivo and characterisation of the purified proteins in vitro. Although DRP2s and SH3P2 arrive similarly late during CME and physically interact, genetic analysis of the sh3p123 triple-mutant and complementation assays with non-SH3P2-interacting DRP2 variants suggests that SH3P2 does not directly recruit DRP2s to the site of endocytosis. These observations imply that despite the presence of many well-conserved endocytic components, plants have acquired a distinct mechanism for CME.}, author = {Gnyliukh, Nataliia and Johnson, Alexander J and Nagel, MK and Monzer, Aline and Babic, David and Hlavata, Annamaria and Alotaibi, SS and Isono, E and Loose, Martin and Friml, Jiří}, issn = {1477-9137}, journal = {Journal of Cell Science}, number = {8}, publisher = {The Company of Biologists}, title = {{Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana}}, doi = {10.1242/jcs.261720}, volume = {137}, year = {2024}, } @inproceedings{15333, abstract = {BUBAAK-SpLit is a tool for dynamically splitting verification tasks into parts that can then be analyzed in parallel. It is built on top of BUBAAK, a tool designed for running combinations of verifiers in parallel. In contrast to BUBAAK, that directly invokes verifiers on the inputs, BUBAAK-SpLit first starts by splitting the input program into multiple modified versions called program splits. During the splitting process, BUBAAK-SpLit utilizes a weak verifier (in our case symbolic execution with a short timelimit) to analyze each generated program split. If the weak verifier fails on a program split, we split this program split again and start the verification process again on the generated program splits. We run the splitting process until a predefined number of hard-to-verify program splits is generated or a splitting limit is reached. During the main verification phase, we run a combination of BUBAAK-LEE and SLOWBEAST in parallel on the remaining unsolved parts of the verification task.}, author = {Chalupa, Marek and Richter, Cedric}, booktitle = {30th International Conference on Tools and Algorithms for the Construction and Analysis of Systems}, isbn = {9783031572555}, issn = {1611-3349}, location = {Luxembourg City, Luxembourg}, pages = {353–358}, publisher = {Springer Nature}, title = {{Bubaak-SpLit: Split what you cannot verify (Competition contribution)}}, doi = {10.1007/978-3-031-57256-2_20}, volume = {14572}, year = {2024}, } @article{15334, abstract = {We consider the sharp interface limit of a Navier-Stokes/Allen Cahn equation in a bounded smooth domain in two space dimensions, in the case of vanishing mobility mε=ε√, where the small parameter ε>0 related to the thickness of the diffuse interface is sent to zero. For well-prepared initial data and sufficiently small times, we rigorously prove convergence to the classical two-phase Navier-Stokes system with surface tension. The idea of the proof is to use asymptotic expansions to construct an approximate solution and to estimate the difference of the exact and approximate solutions with a spectral estimate for the (at the approximate solution) linearized Allen-Cahn operator. In the calculations we use a fractional order ansatz and new ansatz terms in higher orders leading to a suitable ε-scaled and coupled model problem. Moreover, we apply the novel idea of introducing ε-dependent coordinates.}, author = {Abels, Helmut and Fei, Mingwen and Moser, Maximilian}, issn = {1432-0835}, journal = {Calculus of Variations and Partial Differential Equations}, number = {4}, publisher = {Springer Nature}, title = {{Sharp interface limit for a Navier–Stokes/Allen–Cahn system in the case of a vanishing mobility}}, doi = {10.1007/s00526-024-02715-7}, volume = {63}, year = {2024}, } @article{15335, abstract = {Stable matching of neurotransmitters with their receptors is fundamental to synapse function and reliable communication in neural circuits. Presynaptic neurotransmitters regulate the stabilization of postsynaptic transmitter receptors. Whether postsynaptic receptors regulate stabilization of presynaptic transmitters has received less attention. Here, we show that blockade of endogenous postsynaptic acetylcholine receptors (AChR) at the neuromuscular junction destabilizes the cholinergic phenotype in motor neurons and stabilizes an earlier, developmentally transient glutamatergic phenotype. Further, expression of exogenous postsynaptic gamma-aminobutyric acid type A receptors (GABAA receptors) in muscle cells stabilizes an earlier, developmentally transient GABAergic motor neuron phenotype. Both AChR and GABAA receptors are linked to presynaptic neurons through transsynaptic bridges. Knockdown of specific components of these transsynaptic bridges prevents stabilization of the cholinergic or GABAergic phenotypes. Bidirectional communication can enforce a match between transmitter and receptor and ensure the fidelity of synaptic transmission. Our findings suggest a potential role of dysfunctional transmitter receptors in neurological disorders that involve the loss of the presynaptic transmitter.}, author = {Godavarthi, Swetha K. and Hiramoto, Masaki and Ignatyev, Yuri and Levin, Jacqueline B. and Li, Hui Quan and Pratelli, Marta and Borchardt, Jennifer and Czajkowski, Cynthia and Borodinsky, Laura N. and Sweeney, Lora Beatrice Jaeger and Cline, Hollis T. and Spitzer, Nicholas C.}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, number = {15}, publisher = {National Academy of Sciences}, title = {{Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges}}, doi = {10.1073/pnas.2318041121}, volume = {121}, year = {2024}, }