@article{12598, abstract = {Obtaining detailed information about high mountain snowpacks is often limited by insufficient ground-based observations and uncertainty in the (re)distribution of solid precipitation. We utilize high-resolution optical images from Pléiades satellites to generate a snow depth map, at a spatial resolution of 4 m, for a high mountain catchment of central Chile. Results are negatively biased (median difference of −0.22 m) when compared against observations from a terrestrial Light Detection And Ranging scan, though replicate general snow depth variability well. Additionally, the Pléiades dataset is subject to data gaps (17% of total pixels), negative values for shallow snow (12%), and noise on slopes >40–50° (2%). We correct and filter the Pléiades snow depths using surface classification techniques of snow-free areas and a random forest model for data gap filling. Snow depths (with an estimated error of ~0.36 m) average 1.66 m and relate well to topographical parameters such as elevation and northness in a similar way to previous studies. However, estimations of snow depth based upon topography (TOPO) or physically based modeling (DBSM) cannot resolve localized processes (i.e., avalanching or wind scouring) that are detected by Pléiades, even when forced with locally calibrated data. Comparing these alternative model approaches to corrected Pléiades snow depths reveals total snow volume differences between −28% (DBSM) and +54% (TOPO) for the catchment and large differences across most elevation bands. Pléiades represents an important contribution to understanding snow accumulation at sparsely monitored catchments, though ideally requires a careful systematic validation procedure to identify catchment-scale biases and errors in the snow depth derivation.}, author = {Shaw, Thomas E. and Gascoin, Simon and Mendoza, Pablo A. and Pellicciotti, Francesca and McPhee, James}, issn = {1944-7973}, journal = {Water Resources Research}, keywords = {Water Science and Technology}, number = {2}, publisher = {American Geophysical Union}, title = {{Snow depth patterns in a high mountain Andean catchment from satellite optical tristereoscopic remote sensing}}, doi = {10.1029/2019wr024880}, volume = {56}, year = {2020}, } @article{12599, abstract = {Mountains are the water towers of the world, supplying a substantial part of both natural and anthropogenic water demands1,2. They are highly sensitive and prone to climate change3,4, yet their importance and vulnerability have not been quantified at the global scale. Here we present a global water tower index (WTI), which ranks all water towers in terms of their water-supplying role and the downstream dependence of ecosystems and society. For each water tower, we assess its vulnerability related to water stress, governance, hydropolitical tension and future climatic and socio-economic changes. We conclude that the most important (highest WTI) water towers are also among the most vulnerable, and that climatic and socio-economic changes will affect them profoundly. This could negatively impact 1.9 billion people living in (0.3 billion) or directly downstream of (1.6 billion) mountainous areas. Immediate action is required to safeguard the future of the world’s most important and vulnerable water towers.}, author = {Immerzeel, W. W. and Lutz, A. F. and Andrade, M. and Bahl, A. and Biemans, H. and Bolch, T. and Hyde, S. and Brumby, S. and Davies, B. J. and Elmore, A. C. and Emmer, A. and Feng, M. and Fernández, A. and Haritashya, U. and Kargel, J. S. and Koppes, M. and Kraaijenbrink, P. D. A. and Kulkarni, A. V. and Mayewski, P. A. and Nepal, S. and Pacheco, P. and Painter, T. H. and Pellicciotti, Francesca and Rajaram, H. and Rupper, S. and Sinisalo, A. and Shrestha, A. B. and Viviroli, D. and Wada, Y. and Xiao, C. and Yao, T. and Baillie, J. E. M.}, issn = {1476-4687}, journal = {Nature}, number = {7790}, pages = {364--369}, publisher = {Springer Nature}, title = {{Importance and vulnerability of the world’s water towers}}, doi = {10.1038/s41586-019-1822-y}, volume = {577}, year = {2020}, } @article{12939, abstract = {Linear tetrapyrroles, called phyllobilins, are obtained as major catabolites upon chlorophyll degradation. Primarily, colorless phylloleucobilins featuring four deconjugated pyrrole units were identified. Their yellow counterparts, phylloxanthobilins, were discovered more recently. Although the two catabolites differ only by one double bond, physicochemical properties are very distinct. Moreover, the presence of the double bond seems to enhance physiologically relevant bioactivities: in contrast to phylloleucobilin, we identified a potent anti-proliferative activity for a phylloxanthobilin, and show that this natural product induces apoptotic cell death and a cell cycle arrest in cancer cells. Interestingly, upon modifying inactive phylloleucobilin by esterification, an anti-proliferative activity can be observed that increases with the chain lengths of the alkyl esters. We provide first evidence for anti-cancer activity of phyllobilins, report a novel plant source for a phylloxanthobilin, and by using paper spray MS, show that these bioactive yellow chlorophyll catabolites are more prevalent in Nature than previously assumed.}, author = {Karg, Cornelia A. and Wang, Pengyu and Kluibenschedl, Florian and Müller, Thomas and Allmendinger, Lars and Vollmar, Angelika M. and Moser, Simone}, issn = {1434-193X}, journal = {European Journal of Organic Chemistry}, keywords = {Organic Chemistry, Physical and Theoretical Chemistry}, number = {29}, pages = {4499--4509}, publisher = {Wiley}, title = {{Phylloxanthobilins are abundant linear tetrapyrroles from chlorophyll breakdown with activities against cancer cells}}, doi = {10.1002/ejoc.202000692}, volume = {2020}, year = {2020}, } @article{12940, abstract = {Desorption electrospray ionization (DESI), easy ambient sonic-spray ionization (EASI) and low-temperature plasma (LTP) ionization are powerful ambient ionization techniques for mass spectrometry. However, every single method has its limitation in terms of polarity and molecular weight of analyte molecules. After the miniaturization of every possible component of the different ion sources, we finally were able to embed two emitters and an ion transfer tubing into a small, hand-held device. The pen-like interface is connected to the mass spectrometer and a separate control unit via a bundle of flexible tubing and cables. The novel device allows the user to ionize an extended range of chemicals by simple switching between DESI, voltage-free EASI, or LTP ionization as well as to freely move the interface over a surface of interest. A mini camera, which is mounted on the tip of the pen, magnifies the desorption area and enables a simple positioning of the pen. The interface was successfully tested using different types of chemicals, pharmaceuticals, and real life samples. Moreover, the combination of optical data from the camera module and chemical data obtained by mass analysis facilitates a novel type of imaging mass spectrometry, which we name “interactive mass spectrometry imaging (IMSI)”.}, author = {Meisenbichler, Christina and Kluibenschedl, Florian and Müller, Thomas}, issn = {0003-2700}, journal = {Analytical Chemistry}, keywords = {Analytical Chemistry}, number = {21}, pages = {14314--14318}, publisher = {American Chemical Society}, title = {{A 3-in-1 hand-held ambient mass spectrometry interface for identification and 2D localization of chemicals on surfaces}}, doi = {10.1021/acs.analchem.0c02615}, volume = {92}, year = {2020}, } @misc{13056, abstract = {This datasets comprises all data shown in plots of the submitted article "Converting microwave and telecom photons with a silicon photonic nanomechanical interface". Additional raw data are available from the corresponding author on reasonable request.}, author = {Arnold, Georg M and Wulf, Matthias and Barzanjeh, Shabir and Redchenko, Elena and Rueda Sanchez, Alfredo R and Hease, William J and Hassani, Farid and Fink, Johannes M}, publisher = {Zenodo}, title = {{Converting microwave and telecom photons with a silicon photonic nanomechanical interface}}, doi = {10.5281/ZENODO.3961561}, year = {2020}, } @misc{13060, abstract = {Coinfections with multiple pathogens can result in complex within-host dynamics affecting virulence and transmission. Whilst multiple infections are intensively studied in solitary hosts, it is so far unresolved how social host interactions interfere with pathogen competition, and if this depends on coinfection diversity. We studied how the collective disease defenses of ants – their social immunity ­– influence pathogen competition in coinfections of same or different fungal pathogen species. Social immunity reduced virulence for all pathogen combinations, but interfered with spore production only in different-species coinfections. Here, it decreased overall pathogen sporulation success, whilst simultaneously increasing co-sporulation on individual cadavers and maintaining a higher pathogen diversity at the community-level. Mathematical modeling revealed that host sanitary care alone can modulate competitive outcomes between pathogens, giving advantage to fast-germinating, thus less grooming-sensitive ones. Host social interactions can hence modulate infection dynamics in coinfected group members, thereby altering pathogen communities at the host- and population-level.}, author = {Milutinovic, Barbara and Stock, Miriam and Grasse, Anna V and Naderlinger, Elisabeth and Hilbe, Christian and Cremer, Sylvia}, publisher = {Dryad}, title = {{Social immunity modulates competition between coinfecting pathogens}}, doi = {10.5061/DRYAD.CRJDFN318}, year = {2020}, } @misc{13065, abstract = {Domestication is a human-induced selection process that imprints the genomes of domesticated populations over a short evolutionary time scale, and that occurs in a given demographic context. Reconstructing historical gene flow, effective population size changes and their timing is therefore of fundamental interest to understand how plant demography and human selection jointly shape genomic divergence during domestication. Yet, the comparison under a single statistical framework of independent domestication histories across different crop species has been little evaluated so far. Thus, it is unclear whether domestication leads to convergent demographic changes that similarly affect crop genomes. To address this question, we used existing and new transcriptome data on three crop species of Solanaceae (eggplant, pepper and tomato), together with their close wild relatives. We fitted twelve demographic models of increasing complexity on the unfolded joint allele frequency spectrum for each wild/crop pair, and we found evidence for both shared and species-specific demographic processes between species. A convergent history of domestication with gene-flow was inferred for all three species, along with evidence of strong reduction in the effective population size during the cultivation stage of tomato and pepper. The absence of any reduction in size of the crop in eggplant stands out from the classical view of the domestication process; as does the existence of a “protracted period” of management before cultivation. Our results also suggest divergent management strategies of modern cultivars among species as their current demography substantially differs. Finally, the timing of domestication is species-specific and supported by the few historical records available.}, author = {Arnoux, Stephanie and Fraisse, Christelle and Sauvage, Christopher}, publisher = {Dryad}, title = {{VCF files of synonymous SNPs related to: Genomic inference of complex domestication histories in three Solanaceae species}}, doi = {10.5061/DRYAD.Q2BVQ83HD}, year = {2020}, } @misc{13073, abstract = {The Mytilus complex of marine mussel species forms a mosaic of hybrid zones, found across temperate regions of the globe. This allows us to study "replicated" instances of secondary contact between closely-related species. Previous work on this complex has shown that local introgression is both widespread and highly heterogeneous, and has identified SNPs that are outliers of differentiation between lineages. Here, we developed an ancestry-informative panel of such SNPs. We then compared their frequencies in newly-sampled populations, including samples from within the hybrid zones, and parental populations at different distances from the contact. Results show that close to the hybrid zones, some outlier loci are near to fixation for the heterospecific allele, suggesting enhanced local introgression, or the local sweep of a shared ancestral allele. Conversely, genomic cline analyses, treating local parental populations as the reference, reveal a globally high concordance among loci, albeit with a few signals of asymmetric introgression. Enhanced local introgression at specific loci is consistent with the early transfer of adaptive variants after contact, possibly including asymmetric bi-stable variants (Dobzhansky-Muller incompatibilities), or haplotypes loaded with fewer deleterious mutations. Having escaped one barrier, however, these variants can be trapped or delayed at the next barrier, confining the introgression locally. These results shed light on the decay of species barriers during phases of contact.}, author = {Simon, Alexis and Fraisse, Christelle and El Ayari, Tahani and Liautard-Haag, Cathy and Strelkov, Petr and Welch, John and Bierne, Nicolas}, publisher = {Dryad}, title = {{How do species barriers decay? concordance and local introgression in mosaic hybrid zones of mussels}}, doi = {10.5061/DRYAD.R4XGXD29N}, year = {2020}, } @article{13341, abstract = {Scanning nanoscale superconducting quantum interference devices (nanoSQUIDs) are of growing interest for highly sensitive quantitative imaging of magnetic, spintronic, and transport properties of low-dimensional systems. Utilizing specifically designed grooved quartz capillaries pulled into a sharp pipette, we have fabricated the smallest SQUID-on-tip (SOT) devices with effective diameters down to 39 nm. Integration of a resistive shunt in close proximity to the pipette apex combined with self-aligned deposition of In and Sn, have resulted in SOT with a flux noise of 42 n$\Phi_0$Hz$^{-1/2}$, yielding a record low spin noise of 0.29 $\mu_B$Hz$^{-1/2}$. In addition, the new SOTs function at sub-Kelvin temperatures and in high magnetic fields of over 2.5 T. Integrating the SOTs into a scanning probe microscope allowed us to image the stray field of a single Fe$_3$O$_4$ nanocube at 300 mK. Our results show that the easy magnetization axis direction undergoes a transition from the (111) direction at room temperature to an in-plane orientation, which could be attributed to the Verwey phase transition in Fe$_3$O$_4$.}, author = {Anahory, Y. and Naren, H. R. and Lachman, E. O. and Sinai, S. Buhbut and Uri, A. and Embon, L. and Yaakobi, E. and Myasoedov, Y. and Huber, M. E. and Klajn, Rafal and Zeldov, E.}, issn = {2040-3372}, journal = {Nanoscale}, number = {5}, pages = {3174--3182}, publisher = {Royal Society of Chemistry}, title = {{SQUID-on-tip with single-electron spin sensitivity for high-field and ultra-low temperature nanomagnetic imaging}}, doi = {10.1039/C9NR08578E}, volume = {12}, year = {2020}, } @article{19807, abstract = {Microstructures can be carefully designed to reveal the quantum phase of the wave-like nature of electrons in a metal. Here, we report phase-coherent oscillations of out-of-plane magnetoresistance in the layered delafossites PdCoO2 and PtCoO2. The oscillation period is equivalent to that determined by the magnetic flux quantum, h/e, threading an area defined by the atomic interlayer separation and the sample width, where h is Planck’s constant and e is the charge of an electron. The phase of the electron wave function appears robust over length scales exceeding 10 micrometers and persisting up to temperatures of T > 50 kelvin. We show that the experimental signal stems from a periodic field modulation of the out-of-plane hopping. These results demonstrate extraordinary single-particle quantum coherence lengths in delafossites.}, author = {Putzke, Carsten and Bachmann, Maja D. and McGuinness, Philippa and Zhakina, Elina and Sunko, Veronika and Konczykowski, Marcin and Oka, Takashi and Moessner, Roderich and Stern, Ady and König, Markus and Khim, Seunghyun and Mackenzie, Andrew P. and Moll, Philip J.W.}, issn = {1095-9203}, journal = {Science}, number = {6496}, pages = {1234--1238}, publisher = {American Association for the Advancement of Science}, title = {{h/e oscillations in interlayer transport of delafossites}}, doi = {10.1126/science.aay8413}, volume = {368}, year = {2020}, } @article{19812, abstract = {A nearly free electron metal and a Mott insulating state can be thought of as opposite ends of the spectrum of possibilities for the motion of electrons in a solid. Understanding their interaction lies at the heart of the correlated electron problem. In the magnetic oxide metal PdCrO2, nearly free and Mott-localized electrons exist in alternating layers, forming natural heterostructures. Using angle-resolved photoemission spectroscopy, quantitatively supported by a strong coupling analysis, we show that the coupling between these layers leads to an “intertwined” excitation that is a convolution of the charge spectrum of the metallic layer and the spin susceptibility of the Mott layer. Our findings establish PdCrO2 as a model system in which to probe Kondo lattice physics and also open new routes to use the a priori nonmagnetic probe of photoemission to gain insights into the spin susceptibility of correlated electron materials.}, author = {Sunko, Veronika and Mazzola, F. and Kitamura, S. and Khim, S. and Kushwaha, P. and Clark, O. J. and Watson, M. D. and Marković, I. and Biswas, D. and Pourovskii, L. and Kim, T. K. and Lee, T.-L. and Thakur, P. K. and Rosner, H. and Georges, A. and Moessner, R. and Oka, T. and Mackenzie, A. P. and King, P. D. C.}, issn = {2375-2548}, journal = {Science Advances}, number = {6}, publisher = {American Association for the Advancement of Science}, title = {{Probing spin correlations using angle-resolved photoemission in a coupled metallic/Mott insulator system}}, doi = {10.1126/sciadv.aaz0611}, volume = {6}, year = {2020}, } @article{19817, abstract = {We report a combined experimental and theoretical study of the surface and bulk electronic structure of aluminium diboride, a nonsuperconducting sister compound of the superconductor MgB2. We perform angle-resolved photoemission measurements with variable photon energy, and compare them to density functional theory calculations to disentangle the surface and bulk contributions to the measured spectra. Aluminium diboride is known to be aluminium deficient, Al1−𝛿⁢B2, which would be expected to lead to a hole doping as compared to the nominally stoichimoetric compound. Nonetheless, we find that the bulk 𝜎 states, which mediate superconductivity in MgB2, remain more than 600meV below the Fermi level. However, we also observe 𝜎 states originating from the boron terminated surface, with an order of magnitude smaller binding energy of 70meV, and demonstrate how surface hole-doping can bring these across the Fermi level.}, author = {Sunko, Veronika and Milosavljević, D. and Mazzola, F. and Clark, O. J. and Burkhardt, U. and Kim, T. K. and Rosner, H. and Grin, Yu. and Mackenzie, A. P. and King, P. D. C.}, issn = {2469-9969}, journal = {Physical Review B}, number = {3}, publisher = {American Physical Society}, title = {{Surface and bulk electronic structure of aluminium diboride}}, doi = {10.1103/physrevb.102.035143}, volume = {102}, year = {2020}, } @article{19823, abstract = {The delafossite metals PdCoO2, PtCoO2, and PdCrO2 are among the highest conductivity materials known, with low-temperature mean free paths of tens of microns in the best as-grown single crystals. A key question is whether these very low resistive scattering rates result from strongly suppressed backscattering due to special features of the electronic structure or are a consequence of highly unusual levels of crystalline perfection. We report the results of experiments in which high-energy electron irradiation was used to introduce point disorder to the Pd and Pt layers in which the conduction occurs. We obtain the cross section for formation of Frenkel pairs in absolute units, and cross-check our analysis with first-principles calculations of the relevant atomic displacement energies. We observe an increase of resistivity that is linear in defect density with a slope consistent with scattering in the unitary limit. Our results enable us to deduce that the as-grown crystals contain extremely low levels of in-plane defects of approximately 0.001%. This confirms that crystalline perfection is the most important factor in realizing the long mean free paths and highlights how unusual these delafossite metals are in comparison with the vast majority of other multicomponent oxides and alloys. We discuss the implications of our findings for future materials research.}, author = {Sunko, Veronika and McGuinness, P. H. and Chang, C. S. and Zhakina, E. and Khim, S. and Dreyer, C. E. and Konczykowski, M. and Borrmann, H. and Moll, P. J. W. and König, M. and Muller, D. A. and Mackenzie, A. P.}, issn = {2160-3308}, journal = {Physical Review X}, number = {2}, publisher = {American Physical Society}, title = {{Controlled introduction of defects to delafossite metals by electron irradiation}}, doi = {10.1103/physrevx.10.021018}, volume = {10}, year = {2020}, } @inbook{19986, abstract = {For non-probabilistic programs, a key question in static analysis is termination, which asks whether a given program terminates under a given initial condition. In the presence of probabilistic behaviour, there are two fundamental extensions of the termination question: (a) the almost-sure termination question, which asks whether the termination probability is 1; and (b) the bounded-time termination question, which asks whether the expected termination time is bounded. There are many active research directions to address these two questions; one important such direction is the use of martingale theory for termination analysis. In this chapter, we survey the main techniques of the martingale-based approach to the termination analysis of probabilistic programs.}, author = {Chatterjee, Krishnendu and Fu, Hongfei and Novotný, Petr}, booktitle = {Foundations of Probabilistic Programming}, isbn = {9781108488518}, pages = {221--258}, publisher = {Cambridge University Press}, title = {{Termination Analysis of Probabilistic Programs with Martingales}}, doi = {10.1017/9781108770750.008}, year = {2020}, } @article{20766, abstract = {Reversible catalytic reactions operate under thermodynamic control, and thus, establishing a selective catalytic system poses a considerable challenge. Herein, we report a reversible transfer hydrocyanation protocol that exhibits high selectivity for the thermodynamically less favorable branched isomer. Selectivity is achieved by exploiting the lower barrier for C–CN oxidative addition and reductive elimination at benzylic positions in the absence of a cocatalytic Lewis acid. Through the design of a novel type of HCN donor, a practical, branched-selective, HCN-free transfer hydrocyanation was realized. The synthetically useful resolution of a mixture of branched and linear nitrile isomers was also demonstrated to underline the value of reversible and selective transfer reactions. In a broader context, this work demonstrates that high kinetic selectivity can be achieved in reversible transfer reactions, thus opening new horizons for their synthetic applications.}, author = {Bhawal, Benjamin N. and Reisenbauer, Julia and Ehinger, Christian and Morandi, Bill}, issn = {1520-5126}, journal = {Journal of the American Chemical Society}, number = {25}, pages = {10914--10920}, publisher = {American Chemical Society}, title = {{Overcoming selectivity issues in reversible catalysis: A transfer hydrocyanation exhibiting high kinetic control}}, doi = {10.1021/jacs.0c03184}, volume = {142}, year = {2020}, } @article{20806, abstract = {Regulation of phosphatidylinositol phosphates plays a crucial role in signal transduction, membrane trafficking or autophagy. Members of the myotubularin family of lipid phosphatases contribute to phosphoinositide metabolism by counteracting the activity of phosphoinositide kinases. The mechanisms determining their subcellular localization and targeting to specific membrane compartments are still poorly understood. We show here that the inactive phosphatase MTMR9 localizes to the intermediate compartment and to the Golgi apparatus and is able to recruit its active phosphatase partners MTMR6 and MTMR8 to these locations. Furthermore, MTMR8 and MTMR9 co-localize with the small GTPase RAB1A and regulate its localization. Loss of MTMR9 expression compromises the integrity of the Golgi apparatus and results in altered distribution of RAB1A and actin nucleation-promoting factor WHAMM. Loss or overexpression of MTMR9 leads to decreased rate of protein secretion. We demonstrate that secretion of physiologically relevant cargo exemplified by the WNT3A protein is affected after perturbation of MTMR9 levels.}, author = {Doubravská, Lenka and Dostál, Vojtěch and Knop, Filip and Libusová, Lenka and Macůrková, Marie}, issn = {0014-4827}, journal = {Experimental Cell Research}, number = {1}, publisher = {Elsevier}, title = {{Human myotubularin-related protein 9 regulates ER-to-Golgi trafficking and modulates WNT3A secretion}}, doi = {10.1016/j.yexcr.2019.111709}, volume = {386}, year = {2020}, } @article{19306, author = {Kazatskaya, Anna and Yuan, Lisa and Amin-Wetzel, Niko Paresh and Philbrook, Alison and de Bono, Mario and Sengupta, Piali}, issn = {2578-9430}, journal = {microPublication Biology}, number = {9}, publisher = {Caltech Library}, title = {{The URX oxygen-sensing neurons in C. elegans are ciliated}}, doi = {10.17912/MICROPUB.BIOLOGY.000303}, volume = {2020}, year = {2020}, } @article{14891, abstract = {We give the first mathematically rigorous justification of the local density approximation in density functional theory. We provide a quantitative estimate on the difference between the grand-canonical Levy–Lieb energy of a given density (the lowest possible energy of all quantum states having this density) and the integral over the uniform electron gas energy of this density. The error involves gradient terms and justifies the use of the local density approximation in the situation where the density is very flat on sufficiently large regions in space.}, author = {Lewin, Mathieu and Lieb, Elliott H. and Seiringer, Robert}, issn = {2578-5885}, journal = {Pure and Applied Analysis}, number = {1}, pages = {35--73}, publisher = {Mathematical Sciences Publishers}, title = {{ The local density approximation in density functional theory}}, doi = {10.2140/paa.2020.2.35}, volume = {2}, year = {2020}, } @article{15036, abstract = {The assembly of a septin filament requires that homologous monomers must distinguish between one another in establishing appropriate interfaces with their neighbors. To understand this phenomenon at the molecular level, we present the first four crystal structures of heterodimeric septin complexes. We describe in detail the two distinct types of G-interface present within the octameric particles, which must polymerize to form filaments. These are formed between SEPT2 and SEPT6 and between SEPT7 and SEPT3, and their description permits an understanding of the structural basis for the selectivity necessary for correct filament assembly. By replacing SEPT6 by SEPT8 or SEPT11, it is possible to rationalize Kinoshita's postulate, which predicts the exchangeability of septins from within a subgroup. Switches I and II, which in classical small GTPases provide a mechanism for nucleotide-dependent conformational change, have been repurposed in septins to play a fundamental role in molecular recognition. Specifically, it is switch I which holds the key to discriminating between the two different G-interfaces. Moreover, residues which are characteristic for a given subgroup play subtle, but pivotal, roles in guaranteeing that the correct interfaces are formed.}, author = {Rosa, Higor Vinícius Dias and Leonardo, Diego Antonio and Brognara, Gabriel and Brandão-Neto, José and D'Muniz Pereira, Humberto and Araújo, Ana Paula Ulian and Garratt, Richard Charles}, issn = {0022-2836}, journal = {Journal of Molecular Biology}, keywords = {Molecular Biology, Structural Biology}, number = {21}, pages = {5784--5801}, publisher = {Elsevier}, title = {{Molecular recognition at septin interfaces: The switches hold the key}}, doi = {10.1016/j.jmb.2020.09.001}, volume = {432}, year = {2020}, } @article{15037, abstract = {Protein abundance and localization at the plasma membrane (PM) shapes plant development and mediates adaptation to changing environmental conditions. It is regulated by ubiquitination, a post-translational modification crucial for the proper sorting of endocytosed PM proteins to the vacuole for subsequent degradation. To understand the significance and the variety of roles played by this reversible modification, the function of ubiquitin receptors, which translate the ubiquitin signature into a cellular response, needs to be elucidated. In this study, we show that TOL (TOM1-like) proteins function in plants as multivalent ubiquitin receptors, governing ubiquitinated cargo delivery to the vacuole via the conserved Endosomal Sorting Complex Required for Transport (ESCRT) pathway. TOL2 and TOL6 interact with components of the ESCRT machinery and bind to K63-linked ubiquitin via two tandemly arranged conserved ubiquitin-binding domains. Mutation of these domains results not only in a loss of ubiquitin binding but also altered localization, abolishing TOL6 ubiquitin receptor activity. Function and localization of TOL6 is itself regulated by ubiquitination, whereby TOL6 ubiquitination potentially modulates degradation of PM-localized cargoes, assisting in the fine-tuning of the delicate interplay between protein recycling and downregulation. Taken together, our findings demonstrate the function and regulation of a ubiquitin receptor that mediates vacuolar degradation of PM proteins in higher plants.}, author = {Moulinier-Anzola, Jeanette and Schwihla, Maximilian and De-Araújo, Lucinda and Artner, Christina and Jörg, Lisa and Konstantinova, Nataliia and Luschnig, Christian and Korbei, Barbara}, issn = {1674-2052}, journal = {Molecular Plant}, keywords = {Plant Science, Molecular Biology}, number = {5}, pages = {717--731}, publisher = {Elsevier}, title = {{TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants}}, doi = {10.1016/j.molp.2020.02.012}, volume = {13}, year = {2020}, }