@article{1985, abstract = { In Escherichia coli, the pole-to-pole oscillation of the Min proteins directs septum formation to midcell, which is required for symmetric cell division. In vitro, protein waves emerge from the self-organization of MinD, a membrane-binding ATPase, and its activator MinE. For wave propagation, the proteins need to cycle through states of collective membrane binding and unbinding. Although MinD presumably undergoes cooperative membrane attachment, it is unclear how synchronous detachment is coordinated. We used confocal and single-molecule microscopy to elucidate the order of events during Min wave propagation. We propose that protein detachment at the rear of the wave, and the formation of the E-ring, are accomplished by two complementary processes: first, local accumulation of MinE due to rapid rebinding, leading to dynamic instability; and second, a structural change induced by membrane-interaction of MinE in an equimolar MinD-MinE (MinDE) complex, which supports the robustness of pattern formation.}, author = {Martin Loose and Fischer-Friedrich, Elisabeth and Herold, Christoph and Kruse, Karsten and Schwille, Petra }, journal = {Nature Structural and Molecular Biology}, number = {5}, pages = {577 -- 583}, publisher = {Nature Publishing Group}, title = {{Min protein patterns emerge from rapid rebinding and membrane interaction of MinE}}, doi = {10.1038/nsmb.2037}, volume = {18}, year = {2011}, } @article{1986, abstract = {One of the most fundamental features of biological systems is probably their ability to self-organize in space and time on different scales. Despite many elaborate theoretical models of how molecular self-organization can come about, only a few experimental systems of biological origin have so far been rigorously described, due mostly to their inherent complexity. The most promising strategy of modern biophysics is thus to identify minimal biological systems showing self-organized emergent behavior. One of the best-understood examples of protein self-organization, which has recently been successfully reconstituted in vitro, is represented by the oscillations of the Min proteins in Escherichia coli. In this review, we summarize the current understanding of the mechanism of Min protein self-organization in vivo and in vitro. We discuss the potential of the Min oscillations to sense the geometry of the cell and suggest that spontaneous protein waves could be a general means of intracellular organization. We hypothesize that cooperative membrane binding and unbinding, e.g., as an energy-dependent switch, may act as an important regulatory mechanism for protein oscillations and pattern formation in the cell.}, author = {Martin Loose and Kruse, Karsten and Schwille, Petra }, journal = {Annual Review of Biophysics}, number = {1}, pages = {315 -- 336}, publisher = {Annual Reviews}, title = {{Protein self-organization: Lessons from the min system}}, doi = {10.1146/annurev-biophys-042910-155332}, volume = {40}, year = {2011}, } @article{2072, abstract = {Many species have morphologically and genetically differentiated sex chromosomes, such as the XY pair of mammals. Y chromosomes are often highly degenerated and carry few functional genes, so that XY males have only one copy of most Xlinked genes (whereas females have two). As a result, chromosome-wide mechanisms of dosage compensation, such as the mammalian X-inactivation, often evolve to reestablish expression balance. A similar phenomenon is expected in femaleheterogametic species, where ZW females should suffer from imbalances due to W-chromosome degeneration. However, no global dosage compensation mechanisms have been detected in the two independent ZW systems that have been studied systematically (birds and silkworm), leading to the suggestion that lack of global dosage compensation may be a general feature of female-heterogametic species. However, analyses of other independently evolved ZW systems are required to test if this is the case. In this study, we use published genomic and expression data to test for the presence of global dosage compensation in Schistosoma mansoni, a trematode parasite that causes schistosomiasis in humans. We find that Z-linked expression is reduced relative to autosomal expression in females but not males, consistent with incomplete or localized dosage compensation. This gives further support to the theory that female-heterogametic species may not require global mechanisms of dosage compensation.}, author = {Vicoso, Beatriz and Bachtrog, Doris}, journal = {Genome Biology and Evolution}, number = {1}, pages = {230 -- 235}, publisher = {Oxford University Press}, title = {{Lack of global dosage compensation in Schistosoma mansoni, a female-heterogametic parasite}}, doi = {10.1093/gbe/evr010}, volume = {3}, year = {2011}, } @article{1973, abstract = {Complex I is the first and largest enzyme of the respiratory chain, coupling electron transfer between NADH and ubiquinone to the translocation of four protons across the membrane. It has a central role in cellular energy production and has been implicated in many human neurodegenerative diseases. The L-shaped enzyme consists of hydrophilic and membrane domains. Previously, we determined the structure of the hydrophilic domain. Here we report the crystal structure of the Esherichia coli complex I membrane domain at 3.0 Ã. resolution. It includes six subunits, NuoL, NuoM, NuoN, NuoA, NuoJ and NuoK, with 55 transmembrane helices. The fold of the homologous antiporter-like subunits L, M and N is novel, with two inverted structural repeats of five transmembrane helices arranged, unusually, face-to-back. Each repeat includes a discontinuous transmembrane helix and forms half of a channel across the membrane. A network of conserved polar residues connects the two half-channels, completing the proton translocation pathway. Unexpectedly, lysines rather than carboxylate residues act as the main elements of the proton pump in these subunits. The fourth probable proton-translocation channel is at the interface of subunits N, K, J and A. The structure indicates that proton translocation in complex I, uniquely, involves coordinated conformational changes in six symmetrical structural elements.}, author = {Efremov, Rouslan G and Leonid Sazanov}, journal = {Nature}, number = {7361}, pages = {414 -- 421}, publisher = {Nature Publishing Group}, title = {{Structure of the membrane domain of respiratory complex i}}, doi = {10.1038/nature10330}, volume = {476}, year = {2011}, } @article{1974, abstract = {Complex I is the first enzyme of the respiratory chain and plays a central role in cellular energy production. It has been implicated in many human neurodegenerative diseases, as well as in ageing. One of the biggest membrane protein complexes, it is an L-shaped assembly consisting of hydrophilic and membrane domains. Previously, we have determined structures of the hydrophilic domain in several redox states. Last year was marked by fascinating breakthroughs in the understanding of the complete structure. We described the architecture of the membrane domain and of the entire bacterial complex I. X-ray analysis of the larger mitochondrial enzyme has also been published. The core subunits of the bacterial and mitochondrial enzymes have remarkably similar structures. The proposed mechanism of coupling between electron transfer and proton translocation involves long-range conformational changes, coordinated in part by a long α-helix, akin to the coupling rod of a steam engine.}, author = {Efremov, Rouslan G and Leonid Sazanov}, journal = {Current Opinion in Structural Biology}, number = {4}, pages = {532 -- 540}, publisher = {Elsevier}, title = {{Respiratory complex I: 'steam engine' of the cell?}}, doi = {10.1016/j.sbi.2011.07.002}, volume = {21}, year = {2011}, } @article{1975, abstract = {Modern α-proteobacteria are thought to be closely related to the ancient symbiont of eukaryotes, an ancestor of mitochondria. Respiratory complex I from α-proteobacteria and mitochondria is well conserved at the level of the 14 "core" subunits, consistent with that notion. Mitochondrial complex I contains the core subunits, present in all species, and up to 31 "supernumerary" subunits, generally thought to have originated only within eukaryotic lineages. However, the full protein composition of an α-proteobacterial complex I has not been established previously. Here, we report the first purification and characterization of complex I from the α-proteobacterium Paracoccus denitrificans. Single particle electron microscopy shows that the complex has a well defined L-shape. Unexpectedly, in addition to the 14 core subunits, the enzyme also contains homologues of three supernumerary mitochondrial subunits as follows: B17.2, AQDQ/18, and 13 kDa (bovine nomenclature). This finding suggests that evolution of complex I via addition of supernumerary or "accessory" subunits started before the original endosymbiotic event that led to the creation of the eukaryotic cell. It also provides further confirmation that α-proteobacteria are the closest extant relatives of mitochondria.}, author = {Yip, Chui Y and Harbour, Michael E and Jayawardena, Kamburapola G and Fearnley, Ian M and Leonid Sazanov}, journal = {Journal of Biological Chemistry}, number = {7}, pages = {5023 -- 5033}, publisher = {American Society for Biochemistry and Molecular Biology}, title = {{Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme}}, doi = {10.1074/jbc.M110.194993}, volume = {286}, year = {2011}, } @inproceedings{10907, abstract = {This paper presents a method to create a model of an articulated object using the planar motion in an initialization video. The model consists of rigid parts connected by points of articulation. The rigid parts are described by the positions of salient feature-points tracked throughout the video. Following a filtering step that identifies points that belong to different objects, rigid parts are found by a grouping process in a graph pyramid. Valid articulation points are selected by verifying multiple hypotheses for each pair of parts.}, author = {Artner, Nicole M. and Ion, Adrian and Kropatsch, Walter G.}, booktitle = {Graph-Based Representations in Pattern Recognition}, editor = {Jiang, Xiaoyi and Ferrer, Miquel and Torsello, Andrea}, isbn = {9783642208430}, issn = {1611-3349}, location = {Münster, Germany}, pages = {215--224}, publisher = {Springer}, title = {{Spatio-temporal extraction of articulated models in a graph pyramid}}, doi = {10.1007/978-3-642-20844-7_22}, volume = {6658}, year = {2011}, } @article{11094, abstract = {Nuclear pore complexes (NPCs) assemble at the end of mitosis during nuclear envelope (NE) reformation and into an intact NE as cells progress through interphase. Although recent studies have shown that NPC formation occurs by two different molecular mechanisms at two distinct cell cycle stages, little is known about the molecular players that mediate the fusion of the outer and inner nuclear membranes to form pores. In this paper, we provide evidence that the transmembrane nucleoporin (Nup), POM121, but not the Nup107–160 complex, is present at new pore assembly sites at a time that coincides with inner nuclear membrane (INM) and outer nuclear membrane (ONM) fusion. Overexpression of POM121 resulted in juxtaposition of the INM and ONM. Additionally, Sun1, an INM protein that is known to interact with the cytoskeleton, was specifically required for interphase assembly and localized with POM121 at forming pores. We propose a model in which POM121 and Sun1 interact transiently to promote early steps of interphase NPC assembly.}, author = {Talamas, Jessica A. and HETZER, Martin W}, issn = {1540-8140}, journal = {Journal of Cell Biology}, keywords = {Cell Biology}, number = {1}, pages = {27--37}, publisher = {Rockefeller University Press}, title = {{POM121 and Sun1 play a role in early steps of interphase NPC assembly}}, doi = {10.1083/jcb.201012154}, volume = {194}, year = {2011}, } @article{11095, author = {HETZER, Martin W and Cavalli, Giacomo}, issn = {0955-0674}, journal = {Current Opinion in Cell Biology}, keywords = {Cell Biology}, number = {3}, pages = {255--257}, publisher = {Elsevier}, title = {{Editorial overview}}, doi = {10.1016/j.ceb.2011.04.013}, volume = {23}, year = {2011}, } @article{11096, abstract = {As the gatekeepers of the eukaryotic cell nucleus, nuclear pore complexes (NPCs) mediate all molecular trafficking between the nucleoplasm and the cytoplasm. In recent years, transport-independent functions of NPC components, nucleoporins, have been identified including roles in chromatin organization and gene regulation. Here, we summarize our current view of the NPC as a dynamic hub for the integration of chromatin regulation and nuclear trafficking and discuss the functional interplay between nucleoporins and the nuclear genome.}, author = {Liang, Yun and HETZER, Martin W}, issn = {0955-0674}, journal = {Current Opinion in Cell Biology}, keywords = {Cell Biology}, number = {1}, pages = {65--70}, publisher = {Elsevier}, title = {{Functional interactions between nucleoporins and chromatin}}, doi = {10.1016/j.ceb.2010.09.008}, volume = {23}, year = {2011}, } @article{11100, abstract = {Eukaryotic cell function depends on the physical separation of nucleoplasmic and cytoplasmic components by the nuclear envelope (NE). Molecular communication between the two compartments involves active, signal-mediated trafficking, a function that is exclusively performed by nuclear pore complexes (NPCs). The individual NPC components and the mechanisms that are involved in nuclear trafficking are well documented and have become textbook knowledge. However, in addition to their roles as nuclear gatekeepers, NPC components-nucleoporins-have been shown to have critical roles in chromatin organization and gene regulation. These findings have sparked new enthusiasm to study the roles of this multiprotein complex in nuclear organization and explore novel functions that in some cases appear to go beyond a role in transport. Here, we discuss our present view of NPC biogenesis, which is tightly linked to proper cell cycle progression and cell differentiation. In addition, we summarize new data suggesting that NPCs represent dynamic hubs for the integration of gene regulation and nuclear transport processes.}, author = {Capelson, M. and Doucet, C. and HETZER, Martin W}, isbn = {9781936113071}, issn = {0091-7451}, journal = {Cold Spring Harbor Symposia on Quantitative Biology}, keywords = {Genetics, Molecular Biology, Biochemistry}, pages = {585--597}, publisher = {Cold Spring Harbor Laboratory Press}, title = {{Nuclear pore complexes: Guardians of the nuclear genome}}, doi = {10.1101/sqb.2010.75.059}, volume = {75}, year = {2011}, } @article{112, abstract = {Particle beams are important tools for probing atomic and molecular interactions. Here we demonstrate that particle beams also offer a unique opportunity to investigate interactions in macroscopic systems, such as granular media. Motivated by recent experiments on streams of grains that exhibit liquid-like breakup into droplets, we use molecular dynamics simulations to investigate the evolution of a dense stream of macroscopic spheres accelerating out of an opening at the bottom of a reservoir. We show how nanoscale details associated with energy dissipation during collisions modify the stream\'s macroscopic behavior. We find that inelastic collisions collimate the stream, while the presence of short-range attractive interactions drives structure formation. Parameterizing the collision dynamics by the coefficient of restitution (i.e., the ratio of relative velocities before and after impact) and the strength of the cohesive interaction, we map out a spectrum of behaviors that ranges from gaslike jets in which all grains drift apart to liquid-like streams that break into large droplets containing hundreds of grains. We also find a new, intermediate regime in which small aggregates form by capture from the gas phase, similar to what can be observed in molecular beams. Our results show that nearly all aspects of stream behavior are closely related to the velocity gradient associated with vertical free fall. Led by this observation, we propose a simple energy balance model to explain the droplet formation process. The qualitative as well as many quantitative features of the simulations and the model compare well with available experimental data and provide a first quantitative measure of the role of attractions in freely cooling granular streams.}, author = {Waitukaitis, Scott R and Grütjen, Helge and Royer, John and Jaeger, Heinrich}, journal = {Physical Review E}, number = {5}, publisher = {American Physical Society}, title = {{Droplet and cluster formation in freely falling granular streams}}, doi = {10.1103/PhysRevE.83.051302}, volume = {83}, year = {2011}, } @article{10389, abstract = {We perform numerical simulations to study self-assembly of nanoparticles mediated by an elastic planar surface. We show how the nontrivial elastic response to deformations of these surfaces leads to anisotropic interactions between the particles resulting in aggregates having different geometrical features. The morphology of the patterns can be controlled by the mechanical properties of the surface and the strength of the particle adhesion. We use simple scaling arguments to understand the formation of the different structures, and we show how the adhering particles can cause the underlying elastic substrate to wrinkle if two of its opposite edges are clamped. Finally, we discuss the implications of our results and suggest how elastic surfaces could be used in nanofabrication.}, author = {Šarić, Anđela and Cacciuto, Angelo}, issn = {1744-6848}, journal = {Soft Matter}, keywords = {condensed matter physics, general chemistry}, number = {18}, publisher = {Royal Society of Chemistry}, title = {{Soft elastic surfaces as a platform for particle self-assembly}}, doi = {10.1039/c1sm05773a}, volume = {7}, year = {2011}, } @article{1048, abstract = {We produce an ultracold and dense sample of rovibronic ground state Cs 2 molecules close to the regime of quantum degeneracy, in a single hyperfine level, in the presence of an optical lattice. The molecules are individually trapped, in the motional ground state of an optical lattice well, with a lifetime of 8 s. For preparation, we start with a zero-temperature atomic Mott-insulator state with optimized double-site occupancy and efficiently associate weakly-bound dimer molecules on a Feshbach resonance. Despite extremely weak Franck-Condon wavefunction overlap, the molecules are subsequently transferred with >50% efficiency to the rovibronic ground state by a stimulated four-photon process. Our results present a crucial step towards the generation of Bose-Einstein condensates of ground-state molecules and, when suitably generalized to polar heteronuclear molecules such as RbCs, the realization of dipolar many-body quantum-gas phases in periodic potentials.}, author = {Nägerl, Hanns and Mark, Manfred and Haller, Elmar and Gustavsson, Mattias and Hart, Russell and Danzl, Johann G}, journal = {Journal of Physics: Conference Series}, number = {1}, publisher = {IOP Publishing Ltd.}, title = {{Ultracold and dense samples of ground-state molecules in lattice potentials}}, doi = {10.1088/1742-6596/264/1/012015}, volume = {264}, year = {2011}, } @article{1050, abstract = {We present experimentally derived potential curves 1?and spin-orbit interaction functions for the strongly perturbed AΣu+ 3?and bΠu states of the cesium dimer. The results are based on data from several sources. Laser-induced fluorescence Fourier transform spectroscopy (LIF FTS) was used some time ago in the Laboratoire Aimé Cotton primarily to study the XΣg+ state. More recent work at Tsinghua University provides information from moderate 3?resolution spectroscopy on the lowest levels of the bΠ0u± state as well as additional high-resolution data. From Innsbruck University, we have precision data obtained with cold Cs2 molecules. Recent data from Temple University was obtained using the optical-optical double resonance polarization spectroscopy technique, and finally, a group at the University of Latvia has added additional LIF FTS data. In the Hamiltonian matrix, we have used analytic potentials (the expanded Morse oscillator form) with both finite-difference (FD) coupled-channel and discrete variable representation (DVR) calculations of the term values. Fitted diagonal and off-diagonal spin-orbit functions are obtained and compared with ab initio results from Temple and Moscow State universities.}, author = {Bai, Jianmei and Ahmed, Ergin and Beser, Bediha and Guan, Yafei and Kotochigova, Svetlana and Lyyra, Marjatta and Ashman, Seth and Wolfe, Christopher and Huennekens, John and Xie, Feng and Li, Dan and Li, Li and Tamanis, Maris and Ferber, Ruvin and Drozdova, Anastasia and Pazyuk, Elena and Stolyarov, Andrey and Danzl, Johann G and Nägerl, Hanns and Bouloufa, Nadia and Dulieu, Olivier and Amiot, Claude and Salami, Houssam and Bergeman, Thomas}, journal = { Physical Review A - Atomic, Molecular, and Optical Physics}, number = {3}, publisher = {American Physical Society}, title = {{Global analysis of data on the spin-orbit-coupled A 1Σu+ and b 3Πu inf states of Cs2}}, doi = {10.1103/PhysRevA.83.032514}, volume = {83}, year = {2011}, } @article{1051, abstract = {We demonstrate the temporal Talbot effect for trapped matter waves using ultracold atoms in an optical lattice. We investigate the phase evolution of an array of essentially non-interacting matter waves and observe matter-wave collapse and revival in the form of a Talbot interference pattern. By using long expansion times, we image momentum space with sub-recoil resolution, allowing us to observe fractional Talbot fringes up to tenth order.}, author = {Mark, Manfred and Haller, Elmar and Danzl, Johann G and Lauber, Katharina and Gustavsson, Mattias and Nägerl, Hanns}, journal = {New Journal of Physics}, publisher = {IOP Publishing Ltd.}, title = {{Demonstration of the temporal matter-wave Talbot effect for trapped matter waves}}, doi = {10.1088/1367-2630/13/8/085008}, volume = {13}, year = {2011}, } @article{1052, abstract = {The present paper aims at finding optimal parameters for trapping of Cs 2 molecules in optical lattices, with the perspective of creating a quantum degenerate gas of ground-state molecules. We have calculated dynamic polarizabilities of Cs 2 molecules subject to an oscillating electric field, using accurate potential curves and electronic transition dipole moments. We show that for some particular wavelengths of the optical lattice, called "magic wavelengths", the polarizability of the ground-state molecules is equal to the one of a Feshbach molecule. As the creation of the sample of ground-state molecules relies on an adiabatic population transfer from weakly-bound molecules created on a Feshbach resonance, such a coincidence ensures that both the initial and final states are favorably trapped by the lattice light, allowing optimized transfer in agreement with the experimental observation.}, author = {Vexiau, Romain and Bouloufa, Nadia and Aymar, Mireille and Danzl, Johann G and Mark, Manfred and Nägerl, Hanns and Dulieu, Olivier}, journal = {European Physical Journal D}, number = {1-2}, pages = {243 -- 250}, publisher = {Springer}, title = {{Optimal trapping wavelengths of Cs 2 molecules in an optical lattice}}, doi = {10.1140/epjd/e2011-20085-4}, volume = {65}, year = {2011}, } @article{1053, abstract = {We perform precision measurements on a Mott-insulator quantum state of ultracold atoms with tunable interactions. We probe the dependence of the superfluid-to-Mott-insulator transition on the interaction strength and explore the limits of the standard Bose-Hubbard model description. By tuning the on-site interaction energies to values comparable to the interband separation, we are able to quantitatively measure number-dependent shifts in the excitation spectrum caused by effective multibody interactions.}, author = {Mark, Manfred and Haller, Elmar and Lauber, Katharina and Danzl, Johann G and Daley, Andrew and Nägerl, Hanns}, journal = {Physical Review Letters}, number = {17}, publisher = {American Physical Society}, title = {{Precision measurements on a tunable Mott insulator of ultracold atoms}}, doi = {10.1103/PhysRevLett.107.175301}, volume = {107}, year = {2011}, } @article{1054, abstract = {We investigate local three-body correlations for bosonic particles in three dimensions and one dimension as a function of the interaction strength. The three-body correlation function g(3) is determined by measuring the three-body recombination rate in an ultracold gas of Cs atoms. In three dimensions, we measure the dependence of g(3) on the gas parameter in a BEC, finding good agreement with the theoretical prediction accounting for beyond-mean-field effects. In one dimension, we observe a reduction of g( 3) by several orders of magnitude upon increasing interactions from the weakly interacting BEC to the strongly interacting Tonks-Girardeau regime, in good agreement with predictions from the Lieb-Liniger model for all strengths of interaction.}, author = {Haller, Elmar and Rabie, Mahmoud and Mark, Manfred and Danzl, Johann G and Hart, Russell and Lauber, Katharina and Pupillo, Guido and Nägerl, Hanns}, journal = {Physical Review Letters}, number = {23}, publisher = {American Physical Society}, title = {{Three-body correlation functions and recombination rates for bosons in three dimensions and one dimension}}, doi = {10.1103/PhysRevLett.107.230404}, volume = {107}, year = {2011}, } @inbook{2098, abstract = {This chapter presents a method for real-time animation of highly detailed facial expressions based on sparse motion captures data and a limited set of static example poses. The method for real-time animation of highly detailed facial expressions decomposes geometry into large-scale motion and fine-scale details, such as expression wrinkles. Both large- and fine-scale deformation algorithms run entirely on the GPU, and our implementation based on CUDA achieves an overall performance of about 30 fps. The face conveys the most relevant visual characteristics of human identity and expression. Hence, realistic facial animations or interactions with virtual avatars are important for storytelling and gameplay. However, current approaches are either computationally expensive, require very specialized capture hardware, or are extremely labor intensive. At runtime, given an arbitrary facial expression, the algorithm computes the skin strain from the relative distance between marker points and derives fine-scale corrections for the largescale deformation. During gameplay only the sparse set of marker-point positions is transmitted to the GPU. The face animation is entirely computed on the GPU where the resulting mesh can directly be used as input for the rendering stages. This data can be easily obtained by traditional capture hardware. The proposed in-game algorithm is fast. It also is easy to implement and maps well onto programmable GPUs.}, author = {Bernd Bickel and Lang, Manuel}, booktitle = {GPU Computing Gems Emerald Edition}, pages = {413 -- 426}, publisher = {Science Direct}, title = {{From sparse mocap to highly detailed facial animation}}, doi = {10.1016/B978-0-12-384988-5.00027-9}, year = {2011}, }