[{"type":"journal_article","volume":326,"publication":"Cell and Tissue Research","date_updated":"2021-01-12T07:52:24Z","citation":{"ama":"Frotscher M, Gundelfinger E, Jonas PM, Neher E, Seeburg P. The most important recent advances in synapse research from my point of view--and what remains to be done. <i>Cell and Tissue Research</i>. 2006;326(2):203-204. doi:<a href=\"https://doi.org/10.1007/s00441-006-0325-y\">10.1007/s00441-006-0325-y</a>","short":"M. Frotscher, E. Gundelfinger, P.M. Jonas, E. Neher, P. Seeburg, Cell and Tissue Research 326 (2006) 203–4.","mla":"Frotscher, Michael, et al. “The Most Important Recent Advances in Synapse Research from My Point of View--and What Remains to Be Done.” <i>Cell and Tissue Research</i>, vol. 326, no. 2, Springer, 2006, pp. 203–04, doi:<a href=\"https://doi.org/10.1007/s00441-006-0325-y\">10.1007/s00441-006-0325-y</a>.","ista":"Frotscher M, Gundelfinger E, Jonas PM, Neher E, Seeburg P. 2006. The most important recent advances in synapse research from my point of view--and what remains to be done. Cell and Tissue Research. 326(2), 203–4.","ieee":"M. Frotscher, E. Gundelfinger, P. M. Jonas, E. Neher, and P. Seeburg, “The most important recent advances in synapse research from my point of view--and what remains to be done,” <i>Cell and Tissue Research</i>, vol. 326, no. 2. Springer, pp. 203–4, 2006.","chicago":"Frotscher, Michael, Eckart Gundelfinger, Peter M Jonas, Erwin Neher, and Peter Seeburg. “The Most Important Recent Advances in Synapse Research from My Point of View--and What Remains to Be Done.” <i>Cell and Tissue Research</i>. Springer, 2006. <a href=\"https://doi.org/10.1007/s00441-006-0325-y\">https://doi.org/10.1007/s00441-006-0325-y</a>.","apa":"Frotscher, M., Gundelfinger, E., Jonas, P. M., Neher, E., &#38; Seeburg, P. (2006). The most important recent advances in synapse research from my point of view--and what remains to be done. <i>Cell and Tissue Research</i>. Springer. <a href=\"https://doi.org/10.1007/s00441-006-0325-y\">https://doi.org/10.1007/s00441-006-0325-y</a>"},"doi":"10.1007/s00441-006-0325-y","title":"The most important recent advances in synapse research from my point of view--and what remains to be done","quality_controlled":0,"issue":"2","status":"public","month":"01","_id":"3817","year":"2006","author":[{"first_name":"Michael","last_name":"Frotscher","full_name":"Frotscher, Michael"},{"full_name":"Gundelfinger, Eckart","first_name":"Eckart","last_name":"Gundelfinger"},{"first_name":"Peter M","last_name":"Jonas","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Peter Jonas"},{"full_name":"Neher, Erwin","first_name":"Erwin","last_name":"Neher"},{"full_name":"Seeburg, Peter","last_name":"Seeburg","first_name":"Peter"}],"extern":1,"intvolume":"       326","publist_id":"2394","date_created":"2018-12-11T12:05:20Z","day":"01","publisher":"Springer","publication_status":"published","page":"203 - 4","date_published":"2006-01-01T00:00:00Z"},{"date_published":"2006-01-01T00:00:00Z","publication_status":"published","page":"2075 - 81","date_created":"2018-12-11T12:05:20Z","publisher":"Nature Publishing Group","day":"01","author":[{"first_name":"Josef","last_name":"Bischofberger","full_name":"Bischofberger, Josef"},{"full_name":"Engel, Dominique","last_name":"Engel","first_name":"Dominique"},{"full_name":"Li, Liyi","last_name":"Li","first_name":"Liyi"},{"last_name":"Geiger","first_name":"Jörg","full_name":"Geiger, Jörg R"},{"first_name":"Peter M","last_name":"Jonas","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Peter Jonas"}],"extern":1,"publist_id":"2392","intvolume":"         1","year":"2006","status":"public","month":"01","_id":"3818","issue":"4","title":"Patch-clamp recording from mossy fiber terminals in hippocampal slices","quality_controlled":0,"citation":{"mla":"Bischofberger, Josef, et al. “Patch-Clamp Recording from Mossy Fiber Terminals in Hippocampal Slices.” <i>Nature Protocols</i>, vol. 1, no. 4, Nature Publishing Group, 2006, pp. 2075–81, doi:<a href=\"https://doi.org/10.1038/nprot.2006.312 \">10.1038/nprot.2006.312 </a>.","ama":"Bischofberger J, Engel D, Li L, Geiger J, Jonas PM. Patch-clamp recording from mossy fiber terminals in hippocampal slices. <i>Nature Protocols</i>. 2006;1(4):2075-2081. doi:<a href=\"https://doi.org/10.1038/nprot.2006.312 \">10.1038/nprot.2006.312 </a>","short":"J. Bischofberger, D. Engel, L. Li, J. Geiger, P.M. Jonas, Nature Protocols 1 (2006) 2075–81.","ieee":"J. Bischofberger, D. Engel, L. Li, J. Geiger, and P. M. Jonas, “Patch-clamp recording from mossy fiber terminals in hippocampal slices,” <i>Nature Protocols</i>, vol. 1, no. 4. Nature Publishing Group, pp. 2075–81, 2006.","chicago":"Bischofberger, Josef, Dominique Engel, Liyi Li, Jörg Geiger, and Peter M Jonas. “Patch-Clamp Recording from Mossy Fiber Terminals in Hippocampal Slices.” <i>Nature Protocols</i>. Nature Publishing Group, 2006. <a href=\"https://doi.org/10.1038/nprot.2006.312 \">https://doi.org/10.1038/nprot.2006.312 </a>.","apa":"Bischofberger, J., Engel, D., Li, L., Geiger, J., &#38; Jonas, P. M. (2006). Patch-clamp recording from mossy fiber terminals in hippocampal slices. <i>Nature Protocols</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nprot.2006.312 \">https://doi.org/10.1038/nprot.2006.312 </a>","ista":"Bischofberger J, Engel D, Li L, Geiger J, Jonas PM. 2006. Patch-clamp recording from mossy fiber terminals in hippocampal slices. Nature Protocols. 1(4), 2075–81."},"doi":"10.1038/nprot.2006.312 ","date_updated":"2021-01-12T07:52:25Z","publication":"Nature Protocols","abstract":[{"text":"Rigorous analysis of synaptic transmission in the central nervous system requires access to presynaptic terminals. However, cortical terminals have been largely inaccessible to presynaptic patch-clamp recording, due to their small size. Using improved patch-clamp techniques in brain slices, we recorded from mossy fiber terminals in the CA3 region of the hippocampus, which have a diameter of 2-5 microm. The major steps of improvement were the enhanced visibility provided by high-numerical aperture objectives and infrared illumination, the development of vibratomes with minimal vertical blade vibrations and the use of sucrose-based solutions for storage and cutting. Based on these improvements, we describe a protocol that allows us to routinely record from hippocampal mossy fiber boutons. Presynaptic recordings can be obtained in slices from both rats and mice. Presynaptic recordings can be also obtained in slices from transgenic mice in which terminals are labeled with enhanced green fluorescent protein.","lang":"eng"}],"volume":1,"type":"journal_article"},{"publication_status":"published","page":"375 - 389","date_published":"2006-08-10T00:00:00Z","year":"2006","status":"public","month":"08","_id":"3888","date_created":"2018-12-11T12:05:43Z","publisher":"Schloss Dagstuhl - Leibniz-Zentrum für Informatik","day":"10","extern":1,"author":[{"orcid":"0000-0002-4561-241X","full_name":"Krishnendu Chatterjee","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","first_name":"Krishnendu","last_name":"Chatterjee"},{"last_name":"Henzinger","first_name":"Thomas A","id":"40876CD8-F248-11E8-B48F-1D18A9856A87","full_name":"Thomas Henzinger","orcid":"0000−0002−2985−7724"}],"intvolume":"      4137","publist_id":"2278","citation":{"mla":"Chatterjee, Krishnendu, and Thomas A. Henzinger. <i>Strategy Improvement for Stochastic Rabin and Streett Games</i>. Vol. 4137, Schloss Dagstuhl - Leibniz-Zentrum für Informatik, 2006, pp. 375–89, doi:<a href=\"https://doi.org/10.1007/11817949_25\">10.1007/11817949_25</a>.","short":"K. Chatterjee, T.A. Henzinger, in:, Schloss Dagstuhl - Leibniz-Zentrum für Informatik, 2006, pp. 375–389.","ama":"Chatterjee K, Henzinger TA. Strategy improvement for stochastic Rabin and Streett games. In: Vol 4137. Schloss Dagstuhl - Leibniz-Zentrum für Informatik; 2006:375-389. doi:<a href=\"https://doi.org/10.1007/11817949_25\">10.1007/11817949_25</a>","apa":"Chatterjee, K., &#38; Henzinger, T. A. (2006). Strategy improvement for stochastic Rabin and Streett games (Vol. 4137, pp. 375–389). Presented at the CONCUR: Concurrency Theory, Schloss Dagstuhl - Leibniz-Zentrum für Informatik. <a href=\"https://doi.org/10.1007/11817949_25\">https://doi.org/10.1007/11817949_25</a>","chicago":"Chatterjee, Krishnendu, and Thomas A Henzinger. “Strategy Improvement for Stochastic Rabin and Streett Games,” 4137:375–89. Schloss Dagstuhl - Leibniz-Zentrum für Informatik, 2006. <a href=\"https://doi.org/10.1007/11817949_25\">https://doi.org/10.1007/11817949_25</a>.","ieee":"K. Chatterjee and T. A. Henzinger, “Strategy improvement for stochastic Rabin and Streett games,” presented at the CONCUR: Concurrency Theory, 2006, vol. 4137, pp. 375–389.","ista":"Chatterjee K, Henzinger TA. 2006. Strategy improvement for stochastic Rabin and Streett games. CONCUR: Concurrency Theory, LNCS, vol. 4137, 375–389."},"doi":"10.1007/11817949_25","conference":{"name":"CONCUR: Concurrency Theory"},"alternative_title":["LNCS"],"title":"Strategy improvement for stochastic Rabin and Streett games","quality_controlled":0,"volume":4137,"type":"conference","date_updated":"2021-01-12T07:52:58Z","acknowledgement":"This research was supported in part by the NSF grants CCR-0225610 and CCR-0234690, and by the SNSF under the Indo-Swiss Joint Research Programme.","abstract":[{"lang":"eng","text":"A stochastic graph game is played by two players on a game graph with probabilistic transitions. We consider stochastic graph games with omega-regular winning conditions specified as Rabin or Streett objectives. These games are NP-complete and coNP-complete, respectively. The value of the game for a player at a state s given an objective Phi is the maximal probability with which the player can guarantee the satisfaction of Phi from s. We present a strategy-improvement algorithm to compute values in stochastic Rabin games, where an improvement step involves solving Markov decision processes (MDPs) and nonstochastic Rabin games. The algorithm also computes values for stochastic Streett games but does not directly yield an optimal strategy for Streett objectives. We then show how to obtain an optimal strategy for Streett objectives by solving certain nonstochastic Streett games."}]},{"quality_controlled":0,"title":"Algorithms for omega-regular games with imperfect information","alternative_title":["LNCS"],"conference":{"name":"CSL: Computer Science Logic"},"doi":"10.1007/11874683_19","citation":{"short":"K. Chatterjee, L. Doyen, T.A. Henzinger, J. Raskin, in:, Springer, 2006, pp. 287–302.","ama":"Chatterjee K, Doyen L, Henzinger TA, Raskin J. Algorithms for omega-regular games with imperfect information. In: Vol 4207. Springer; 2006:287-302. doi:<a href=\"https://doi.org/10.1007/11874683_19\">10.1007/11874683_19</a>","mla":"Chatterjee, Krishnendu, et al. <i>Algorithms for Omega-Regular Games with Imperfect Information</i>. Vol. 4207, Springer, 2006, pp. 287–302, doi:<a href=\"https://doi.org/10.1007/11874683_19\">10.1007/11874683_19</a>.","ista":"Chatterjee K, Doyen L, Henzinger TA, Raskin J. 2006. Algorithms for omega-regular games with imperfect information. CSL: Computer Science Logic, LNCS, vol. 4207, 287–302.","apa":"Chatterjee, K., Doyen, L., Henzinger, T. A., &#38; Raskin, J. (2006). Algorithms for omega-regular games with imperfect information (Vol. 4207, pp. 287–302). Presented at the CSL: Computer Science Logic, Springer. <a href=\"https://doi.org/10.1007/11874683_19\">https://doi.org/10.1007/11874683_19</a>","ieee":"K. Chatterjee, L. Doyen, T. A. Henzinger, and J. Raskin, “Algorithms for omega-regular games with imperfect information,” presented at the CSL: Computer Science Logic, 2006, vol. 4207, pp. 287–302.","chicago":"Chatterjee, Krishnendu, Laurent Doyen, Thomas A Henzinger, and Jean Raskin. “Algorithms for Omega-Regular Games with Imperfect Information,” 4207:287–302. Springer, 2006. <a href=\"https://doi.org/10.1007/11874683_19\">https://doi.org/10.1007/11874683_19</a>."},"abstract":[{"lang":"eng","text":"We study observation-based strategies for two-player turn-based games on graphs with omega-regular objectives. An observation-based strategy relies on imperfect information about the history of a play, namely, on the past sequence of observations. Such games occur in the synthesis of a controller that does not see the private state of the plant. Our main results are twofold. First, we give a fixed-point algorithm for computing the set of states from which a player can win with a deterministic observation-based strategy for any omega-regular objective. The fixed point is computed in the lattice of antichains of state sets. This algorithm has the advantages of being directed by the objective and of avoiding an explicit subset construction on the game graph. Second, we give an algorithm for computing the set of states from which a player can win with probability 1 with a randomized observation-based strategy for a Buchi objective. This set is of interest because in the absence of perfect information, randomized strategies are more powerful than deterministic ones. We show that our algorithms are optimal by proving matching lower bounds."}],"acknowledgement":"This research was supported in part by the NSF grants CCR-0225610 and CCR-0234690, by the SNSF under the Indo-Swiss Joint Research Programme, and by the FRFC project “Centre Fédéré en Vérification” funded by the FNRS under grant 2.4530.02.","date_updated":"2021-01-12T07:52:59Z","type":"conference","volume":4207,"date_published":"2006-11-13T00:00:00Z","page":"287 - 302","publication_status":"published","intvolume":"      4207","publist_id":"2276","author":[{"orcid":"0000-0002-4561-241X","full_name":"Krishnendu Chatterjee","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","first_name":"Krishnendu","last_name":"Chatterjee"},{"first_name":"Laurent","last_name":"Doyen","full_name":"Doyen, Laurent"},{"orcid":"0000−0002−2985−7724","full_name":"Thomas Henzinger","id":"40876CD8-F248-11E8-B48F-1D18A9856A87","first_name":"Thomas A","last_name":"Henzinger"},{"first_name":"Jean","last_name":"Raskin","full_name":"Raskin, Jean-François"}],"extern":1,"publisher":"Springer","day":"13","date_created":"2018-12-11T12:05:43Z","_id":"3889","month":"11","status":"public","year":"2006"},{"conference":{"name":"SODA: Symposium on Discrete Algorithms"},"title":"The complexity of quantitative concurrent parity games","quality_controlled":0,"citation":{"short":"K. Chatterjee, L. De Alfaro, T.A. Henzinger, in:, SIAM, 2006, pp. 678–687.","ama":"Chatterjee K, De Alfaro L, Henzinger TA. The complexity of quantitative concurrent parity games. In: SIAM; 2006:678-687. doi:<a href=\"https://doi.org/10.1145/1109557.1109631\">10.1145/1109557.1109631</a>","mla":"Chatterjee, Krishnendu, et al. <i>The Complexity of Quantitative Concurrent Parity Games</i>. SIAM, 2006, pp. 678–87, doi:<a href=\"https://doi.org/10.1145/1109557.1109631\">10.1145/1109557.1109631</a>.","ista":"Chatterjee K, De Alfaro L, Henzinger TA. 2006. The complexity of quantitative concurrent parity games. SODA: Symposium on Discrete Algorithms, 678–687.","apa":"Chatterjee, K., De Alfaro, L., &#38; Henzinger, T. A. (2006). The complexity of quantitative concurrent parity games (pp. 678–687). Presented at the SODA: Symposium on Discrete Algorithms, SIAM. <a href=\"https://doi.org/10.1145/1109557.1109631\">https://doi.org/10.1145/1109557.1109631</a>","ieee":"K. Chatterjee, L. De Alfaro, and T. A. Henzinger, “The complexity of quantitative concurrent parity games,” presented at the SODA: Symposium on Discrete Algorithms, 2006, pp. 678–687.","chicago":"Chatterjee, Krishnendu, Luca De Alfaro, and Thomas A Henzinger. “The Complexity of Quantitative Concurrent Parity Games,” 678–87. SIAM, 2006. <a href=\"https://doi.org/10.1145/1109557.1109631\">https://doi.org/10.1145/1109557.1109631</a>."},"doi":"10.1145/1109557.1109631","date_updated":"2021-01-12T07:52:59Z","acknowledgement":"This research was supported in part by the AFOSR MURI grant F49620-00-1-0327 and the NSF ITR grant CCR-0225610.","abstract":[{"lang":"eng","text":"We consider two-player infinite games played on graphs. The games are concurrent, in that at each state the players choose their moves simultaneously and independently, and stochastic, in that the moves determine a probability distribution for the successor state. The value of a game is the maximal probability with which a player can guarantee the satisfaction of her objective. We show that the values of concurrent games with w-regular objectives expressed as parity conditions can be decided in NP boolean AND coNP. This result substantially improves the best known previous bound of 3EXPTIME. It also shows that the full class of concurrent parity games is no harder than the special case of turn-based stochastic reachability games, for which NP boolean AND coNP is the best known bound. While the previous, more restricted NP boolean AND coNP results for graph games relied on the existence of particularly simple (pure memoryless) optimal strategies, in concurrent games with parity objectives optimal strategies may not exist, and epsilon-optimal strategies (which achieve the value of the game within a parameter epsilon &gt; 0) require in general both randomization and infinite memory. Hence our proof must rely on a more detailed analysis of strategies and, in addition to the main result, yields two results that are interesting on their own. First, we show that there exist epsilon-optimal strategies that in the limit coincide with memoryless strategies; this parallels the celebrated result of Mertens-Neyman for concurrent games with limit-average objectives. Second, we complete the characterization of the memory requirements for epsilon-optimal strategies for concurrent games with parity conditions, by showing that memoryless strategies suffice for epsilon-optimality for coBachi conditions."}],"type":"conference","date_published":"2006-01-01T00:00:00Z","page":"678 - 687","publication_status":"published","date_created":"2018-12-11T12:05:43Z","publisher":"SIAM","day":"01","extern":1,"author":[{"orcid":"0000-0002-4561-241X","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","full_name":"Krishnendu Chatterjee","first_name":"Krishnendu","last_name":"Chatterjee"},{"first_name":"Luca","last_name":"De Alfaro","full_name":"de Alfaro, Luca"},{"orcid":"0000−0002−2985−7724","full_name":"Thomas Henzinger","id":"40876CD8-F248-11E8-B48F-1D18A9856A87","first_name":"Thomas A","last_name":"Henzinger"}],"publist_id":"2273","year":"2006","status":"public","_id":"3890","month":"01"},{"type":"conference","volume":4207,"abstract":[{"text":"We study infinite stochastic games played by two-players over a finite state space, with objectives specified by sets of infinite traces. The games are concurrent (players make moves simultaneously and independently), stochastic (the next state is determined by a probability distribution that depends on the current state and chosen moves of the players) and infinite (proceeds for infinite number of rounds). The analysis of concurrent stochastic games can be classified into: quantitative analysis, analyzing the optimum value of the game; and qualitative analysis, analyzing the set of states with optimum value 1. We consider concurrent games with tail objectives, i.e., objectives that are independent of the finite-prefix of traces, and show that the class of tail objectives are strictly richer than the omega-regular objectives. We develop new proof techniques to extend several properties of concurrent games with omega-regular objectives to concurrent games with tail objectives. We prove the positive limit-one property for tail objectives, that states for all concurrent games if the optimum value for a player is positive for a tail objective Phi at some state, then there is a state where the optimum value is 1 for Phi, for the player. We also show that the optimum values of zero-sum (strictly conflicting objectives) games with tail objectives can be related to equilibrium values of nonzero-sum (not strictly conflicting objectives) games with simpler reachability objectives. A consequence of our analysis presents a polynomial time reduction of the quantitative analysis of tail objectives to the qualitative analysis for the sub-class of one-player stochastic games (Markov decision processes).","lang":"eng"}],"date_updated":"2021-01-12T07:53:00Z","citation":{"ista":"Chatterjee K. 2006. Concurrent games with tail objectives. CSL: Computer Science Logic, LNCS , vol. 4207, 256–270.","apa":"Chatterjee, K. (2006). Concurrent games with tail objectives (Vol. 4207, pp. 256–270). Presented at the CSL: Computer Science Logic, Springer. <a href=\"https://doi.org/10.1007/11874683_17\">https://doi.org/10.1007/11874683_17</a>","chicago":"Chatterjee, Krishnendu. “Concurrent Games with Tail Objectives,” 4207:256–70. Springer, 2006. <a href=\"https://doi.org/10.1007/11874683_17\">https://doi.org/10.1007/11874683_17</a>.","ieee":"K. Chatterjee, “Concurrent games with tail objectives,” presented at the CSL: Computer Science Logic, 2006, vol. 4207, pp. 256–270.","short":"K. Chatterjee, in:, Springer, 2006, pp. 256–270.","ama":"Chatterjee K. Concurrent games with tail objectives. In: Vol 4207. Springer; 2006:256-270. doi:<a href=\"https://doi.org/10.1007/11874683_17\">10.1007/11874683_17</a>","mla":"Chatterjee, Krishnendu. <i>Concurrent Games with Tail Objectives</i>. Vol. 4207, Springer, 2006, pp. 256–70, doi:<a href=\"https://doi.org/10.1007/11874683_17\">10.1007/11874683_17</a>."},"doi":"10.1007/11874683_17","title":"Concurrent games with tail objectives","quality_controlled":0,"alternative_title":["LNCS "],"conference":{"name":"CSL: Computer Science Logic"},"status":"public","_id":"3891","month":"09","year":"2006","author":[{"full_name":"Krishnendu Chatterjee","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4561-241X","last_name":"Chatterjee","first_name":"Krishnendu"}],"extern":1,"intvolume":"      4207","publist_id":"2272","date_created":"2018-12-11T12:05:44Z","day":"28","publisher":"Springer","page":"256 - 270","publication_status":"published","date_published":"2006-09-28T00:00:00Z"},{"year":"2006","status":"public","month":"02","_id":"3908","date_created":"2018-12-11T12:05:49Z","publisher":"Springer","day":"01","extern":"1","author":[{"full_name":"Ustinova, Jana","first_name":"Jana","last_name":"Ustinova"},{"full_name":"Achmann, Roland","last_name":"Achmann","first_name":"Roland"},{"full_name":"Cremer, Sylvia","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2193-3868","last_name":"Cremer","first_name":"Sylvia"},{"last_name":"Mayer","first_name":"Frieder","full_name":"Mayer, Frieder"}],"intvolume":"        62","publist_id":"2242","publication_status":"published","page":"158 - 167","date_published":"2006-02-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","volume":62,"date_updated":"2021-01-12T07:53:07Z","abstract":[{"lang":"eng","text":"It is commonly believed that both the average length and the frequency of microsatellites correlate with genome size. We have estimated the frequency and the average length for 69 perfect dinucleotide microsatellites in an insect with an exceptionally large genome: Chorthippus biguttulus (Orthoptera, Acrididae). Dinucleotide microsatellites are not more frequent in C. biguttulus, but repeat arrays are 1.4 to 2 times longer than in other insect species. The average repeat number in C. biguttulus lies in the range of higher vertebrates. Natural populations are highly variable. At least 30 alleles per locus were found and the expected heterozygosity is above 0.95 at all three loci studied. In contrast, the observed heterozygosity is much lower (≤0.51), which could be caused by long null alleles."}],"publication":"Journal of Molecular Evolution","language":[{"iso":"eng"}],"citation":{"ama":"Ustinova J, Achmann R, Cremer S, Mayer F. Long repeats in a huge gemome: microsatellite loci in the grasshopper Chorthippus biguttulus. <i>Journal of Molecular Evolution</i>. 2006;62(2):158-167. doi:<a href=\"https://doi.org/10.1007/s00239-005-0022-6\">10.1007/s00239-005-0022-6</a>","short":"J. Ustinova, R. Achmann, S. Cremer, F. Mayer, Journal of Molecular Evolution 62 (2006) 158–167.","mla":"Ustinova, Jana, et al. “Long Repeats in a Huge Gemome: Microsatellite Loci in the Grasshopper Chorthippus Biguttulus.” <i>Journal of Molecular Evolution</i>, vol. 62, no. 2, Springer, 2006, pp. 158–67, doi:<a href=\"https://doi.org/10.1007/s00239-005-0022-6\">10.1007/s00239-005-0022-6</a>.","ista":"Ustinova J, Achmann R, Cremer S, Mayer F. 2006. Long repeats in a huge gemome: microsatellite loci in the grasshopper Chorthippus biguttulus. Journal of Molecular Evolution. 62(2), 158–167.","ieee":"J. Ustinova, R. Achmann, S. Cremer, and F. Mayer, “Long repeats in a huge gemome: microsatellite loci in the grasshopper Chorthippus biguttulus,” <i>Journal of Molecular Evolution</i>, vol. 62, no. 2. Springer, pp. 158–167, 2006.","chicago":"Ustinova, Jana, Roland Achmann, Sylvia Cremer, and Frieder Mayer. “Long Repeats in a Huge Gemome: Microsatellite Loci in the Grasshopper Chorthippus Biguttulus.” <i>Journal of Molecular Evolution</i>. Springer, 2006. <a href=\"https://doi.org/10.1007/s00239-005-0022-6\">https://doi.org/10.1007/s00239-005-0022-6</a>.","apa":"Ustinova, J., Achmann, R., Cremer, S., &#38; Mayer, F. (2006). Long repeats in a huge gemome: microsatellite loci in the grasshopper Chorthippus biguttulus. <i>Journal of Molecular Evolution</i>. Springer. <a href=\"https://doi.org/10.1007/s00239-005-0022-6\">https://doi.org/10.1007/s00239-005-0022-6</a>"},"oa_version":"None","doi":"10.1007/s00239-005-0022-6","issue":"2","title":"Long repeats in a huge gemome: microsatellite loci in the grasshopper Chorthippus biguttulus"},{"title":"Attack of the invasive garden ant: aggression behaviour of Lasius neglectus (Hymenoptera: Formicidae) against native Lasius species in Spain","citation":{"apa":"Cremer, S., Ugelvig, L. V., Lommen, S., Petersen, K., &#38; Pedersen, J. (2006). Attack of the invasive garden ant: aggression behaviour of Lasius neglectus (Hymenoptera: Formicidae) against native Lasius species in Spain. <i>Myrmecological News</i>. Österreichische Gesellschaft für Entomofaunistik.","chicago":"Cremer, Sylvia, Line V Ugelvig, Suzanne Lommen, Klaus Petersen, and Jes Pedersen. “Attack of the Invasive Garden Ant: Aggression Behaviour of Lasius Neglectus (Hymenoptera: Formicidae) against Native Lasius Species in Spain.” <i>Myrmecological News</i>. Österreichische Gesellschaft für Entomofaunistik, 2006.","ieee":"S. Cremer, L. V. Ugelvig, S. Lommen, K. Petersen, and J. Pedersen, “Attack of the invasive garden ant: aggression behaviour of Lasius neglectus (Hymenoptera: Formicidae) against native Lasius species in Spain,” <i>Myrmecological News</i>, vol. 9. Österreichische Gesellschaft für Entomofaunistik, pp. 13–19, 2006.","ista":"Cremer S, Ugelvig LV, Lommen S, Petersen K, Pedersen J. 2006. Attack of the invasive garden ant: aggression behaviour of Lasius neglectus (Hymenoptera: Formicidae) against native Lasius species in Spain. Myrmecological News. 9, 13–19.","mla":"Cremer, Sylvia, et al. “Attack of the Invasive Garden Ant: Aggression Behaviour of Lasius Neglectus (Hymenoptera: Formicidae) against Native Lasius Species in Spain.” <i>Myrmecological News</i>, vol. 9, Österreichische Gesellschaft für Entomofaunistik, 2006, pp. 13–19.","short":"S. Cremer, L.V. Ugelvig, S. Lommen, K. Petersen, J. Pedersen, Myrmecological News 9 (2006) 13–19.","ama":"Cremer S, Ugelvig LV, Lommen S, Petersen K, Pedersen J. Attack of the invasive garden ant: aggression behaviour of Lasius neglectus (Hymenoptera: Formicidae) against native Lasius species in Spain. <i>Myrmecological News</i>. 2006;9:13-19."},"oa_version":"None","date_updated":"2021-01-12T07:53:09Z","abstract":[{"lang":"eng","text":"Invasive species often dramatically change native species communities by directly and indirectly out-competing native species. We studied the direct interference abilities of the invasive garden ant, Lasius neglectus VAN LOON, BOOMSMA &amp; ANDRÁSFALVY, 1990, by performing one-to-one aggression tests of L. neglectus workers towards three native Lasius ant species that occur at the edge of a L. neglectus supercolony in Seva, Spain. Our results show that L. neglectus is highly aggressive against all three native Lasius species tested (L. grandis FOREL, 1909, L. emarginatus (OLIVIER, 1792), and L. cinereus SEIFERT, 1992), expressed as a higher attack rate of L. neglectus and behavioural dominance throughout the aggressive encounters. Attacks of L. neglectus were performed fastest and most frequent against L. grandis, and also the highest antennation frequencies were observed in encounters between these two species. This could be due to the largest difference in body size, or due to a greater overlap in ecological niche between L. neglectus and L. grandis compared to the other two native species. There was only weak support for L. neglectus workers from the periphery of the supercolony to be more aggressive relative to workers from the centre, even though the former encounter native ant species on a daily basis at the edge of the supercolony."}],"publication":"Myrmecological News","language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":9,"type":"journal_article","date_published":"2006-12-01T00:00:00Z","page":"13 - 19","publication_status":"published","date_created":"2018-12-11T12:05:51Z","day":"01","publisher":"Österreichische Gesellschaft für Entomofaunistik","extern":"1","author":[{"full_name":"Cremer, Sylvia","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2193-3868","last_name":"Cremer","first_name":"Sylvia"},{"orcid":"0000-0003-1832-8883","full_name":"Ugelvig, Line V","id":"3DC97C8E-F248-11E8-B48F-1D18A9856A87","first_name":"Line V","last_name":"Ugelvig"},{"first_name":"Suzanne","last_name":"Lommen","full_name":"Lommen, Suzanne"},{"full_name":"Petersen, Klaus","last_name":"Petersen","first_name":"Klaus"},{"first_name":"Jes","last_name":"Pedersen","full_name":"Pedersen, Jes"}],"publist_id":"2239","intvolume":"         9","year":"2006","status":"public","_id":"3912","month":"12"},{"citation":{"mla":"Heinze, Jürgen, et al. “Stealthy Invaders: The Biology of Cardiocondyla Tramp Ants.” <i>Insectes Sociaux</i>, vol. 53, no. 1, Springer, 2006, pp. 1–7, doi:<a href=\"https://doi.org/10.1007/s00040-005-0847-4\">10.1007/s00040-005-0847-4</a>.","ama":"Heinze J, Cremer S, Eckl N, Schrempf A. Stealthy invaders: the biology of Cardiocondyla tramp ants. <i>Insectes Sociaux</i>. 2006;53(1):1-7. doi:<a href=\"https://doi.org/10.1007/s00040-005-0847-4\">10.1007/s00040-005-0847-4</a>","short":"J. Heinze, S. Cremer, N. Eckl, A. Schrempf, Insectes Sociaux 53 (2006) 1–7.","chicago":"Heinze, Jürgen, Sylvia Cremer, Norbert Eckl, and Alexandra Schrempf. “Stealthy Invaders: The Biology of Cardiocondyla Tramp Ants.” <i>Insectes Sociaux</i>. Springer, 2006. <a href=\"https://doi.org/10.1007/s00040-005-0847-4\">https://doi.org/10.1007/s00040-005-0847-4</a>.","ieee":"J. Heinze, S. Cremer, N. Eckl, and A. Schrempf, “Stealthy invaders: the biology of Cardiocondyla tramp ants,” <i>Insectes Sociaux</i>, vol. 53, no. 1. Springer, pp. 1–7, 2006.","apa":"Heinze, J., Cremer, S., Eckl, N., &#38; Schrempf, A. (2006). Stealthy invaders: the biology of Cardiocondyla tramp ants. <i>Insectes Sociaux</i>. Springer. <a href=\"https://doi.org/10.1007/s00040-005-0847-4\">https://doi.org/10.1007/s00040-005-0847-4</a>","ista":"Heinze J, Cremer S, Eckl N, Schrempf A. 2006. Stealthy invaders: the biology of Cardiocondyla tramp ants. Insectes Sociaux. 53(1), 1–7."},"doi":"10.1007/s00040-005-0847-4","oa_version":"None","issue":"1","title":"Stealthy invaders: the biology of Cardiocondyla tramp ants","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","volume":53,"date_updated":"2021-01-12T07:53:09Z","language":[{"iso":"eng"}],"abstract":[{"text":"Many invasive ant species, such as the Argentine ant or the red imported fire ant, have huge colonies with thousands of mass-foraging workers, which quickly monopolise resources and therefore represent a considerable threat to the native ant fauna. Cardiocondyla obscurior and several other species of this myrmicine genus have similarly been transferred throughout the tropics by human activities. However, because their colonies are tiny and workers forage solitarily, Cardiocondyla are often not recognized as successful invaders. Here, we document that the life history of Cardiocondyla closely resembles that of the more conspicuous tramp species, with polygyny, intranidal mating, budding, worker sterility, low genetic variability, and possibly also unicoloniality. Given that introduced Cardiocondyla may locally reach a very high population density, the effects of these stealthy invaders on the native arthropod fauna should receive more attention.","lang":"eng"}],"publication":"Insectes Sociaux","page":"1 - 7","publication_status":"published","date_published":"2006-02-01T00:00:00Z","year":"2006","_id":"3913","month":"02","status":"public","day":"01","publisher":"Springer","date_created":"2018-12-11T12:05:51Z","publist_id":"2240","intvolume":"        53","extern":"1","author":[{"full_name":"Heinze, Jürgen","last_name":"Heinze","first_name":"Jürgen"},{"first_name":"Sylvia","last_name":"Cremer","orcid":"0000-0002-2193-3868","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","full_name":"Cremer, Sylvia"},{"last_name":"Eckl","first_name":"Norbert","full_name":"Eckl, Norbert"},{"last_name":"Schrempf","first_name":"Alexandra","full_name":"Schrempf, Alexandra"}]},{"publication_status":"published","page":"82 - 87","date_published":"2006-08-29T00:00:00Z","status":"public","_id":"3914","month":"08","year":"2006","extern":"1","author":[{"full_name":"Moder, Karl","last_name":"Moder","first_name":"Karl"},{"full_name":"Schlick Steiner, Birgit","last_name":"Schlick Steiner","first_name":"Birgit"},{"full_name":"Steiner, Florian","first_name":"Florian","last_name":"Steiner"},{"first_name":"Sylvia","last_name":"Cremer","orcid":"0000-0002-2193-3868","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","full_name":"Cremer, Sylvia"},{"full_name":"Christian, Erhard","first_name":"Erhard","last_name":"Christian"},{"last_name":"Seifert","first_name":"Bernhard","full_name":"Seifert, Bernhard"}],"publist_id":"2241","intvolume":"        45","date_created":"2018-12-11T12:05:52Z","day":"29","publisher":"Wiley-Blackwell","citation":{"mla":"Moder, Karl, et al. “Optimal Species Distinction by Discriminant Analysis: Comparing Established Methods of Character Selection with a Combination Procedure Using Ant Morphometrics as a Case Study.” <i>Journal of Zoological Systematics and Evolutionary Research</i>, vol. 45, no. 1, Wiley-Blackwell, 2006, pp. 82–87, doi:<a href=\"https://doi.org/10.1111/j.1439-0469.2006.00372.x\">10.1111/j.1439-0469.2006.00372.x</a>.","short":"K. Moder, B. Schlick Steiner, F. Steiner, S. Cremer, E. Christian, B. Seifert, Journal of Zoological Systematics and Evolutionary Research 45 (2006) 82–87.","ama":"Moder K, Schlick Steiner B, Steiner F, Cremer S, Christian E, Seifert B. Optimal species distinction by discriminant analysis: comparing established methods of character selection with a combination procedure using ant morphometrics as a case study. <i>Journal of Zoological Systematics and Evolutionary Research</i>. 2006;45(1):82-87. doi:<a href=\"https://doi.org/10.1111/j.1439-0469.2006.00372.x\">10.1111/j.1439-0469.2006.00372.x</a>","apa":"Moder, K., Schlick Steiner, B., Steiner, F., Cremer, S., Christian, E., &#38; Seifert, B. (2006). Optimal species distinction by discriminant analysis: comparing established methods of character selection with a combination procedure using ant morphometrics as a case study. <i>Journal of Zoological Systematics and Evolutionary Research</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/j.1439-0469.2006.00372.x\">https://doi.org/10.1111/j.1439-0469.2006.00372.x</a>","ieee":"K. Moder, B. Schlick Steiner, F. Steiner, S. Cremer, E. Christian, and B. Seifert, “Optimal species distinction by discriminant analysis: comparing established methods of character selection with a combination procedure using ant morphometrics as a case study,” <i>Journal of Zoological Systematics and Evolutionary Research</i>, vol. 45, no. 1. Wiley-Blackwell, pp. 82–87, 2006.","chicago":"Moder, Karl, Birgit Schlick Steiner, Florian Steiner, Sylvia Cremer, Erhard Christian, and Bernhard Seifert. “Optimal Species Distinction by Discriminant Analysis: Comparing Established Methods of Character Selection with a Combination Procedure Using Ant Morphometrics as a Case Study.” <i>Journal of Zoological Systematics and Evolutionary Research</i>. Wiley-Blackwell, 2006. <a href=\"https://doi.org/10.1111/j.1439-0469.2006.00372.x\">https://doi.org/10.1111/j.1439-0469.2006.00372.x</a>.","ista":"Moder K, Schlick Steiner B, Steiner F, Cremer S, Christian E, Seifert B. 2006. Optimal species distinction by discriminant analysis: comparing established methods of character selection with a combination procedure using ant morphometrics as a case study. Journal of Zoological Systematics and Evolutionary Research. 45(1), 82–87."},"oa_version":"None","doi":"10.1111/j.1439-0469.2006.00372.x","title":"Optimal species distinction by discriminant analysis: comparing established methods of character selection with a combination procedure using ant morphometrics as a case study","issue":"1","type":"journal_article","volume":45,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"text":"We compare the performances of established means of character selection for discriminant analysis in species distinction with a combination procedure for finding the optimal character combination (minimum classification error, minimum number of required characters), using morphometric data sets from the ant genera Cardiocondyla, Lasius and Tetramorium. The established methods are empirical character selection as well as forward selection, backward elimination and stepwise selection of discriminant analysis. The combination procedure is clearly superior to the established methods of character selection, and is widely applicable.","lang":"eng"}],"publication":"Journal of Zoological Systematics and Evolutionary Research","language":[{"iso":"eng"}],"date_updated":"2021-01-12T07:53:10Z"},{"citation":{"chicago":"Henic, Emir, Michael K Sixt, Stefan Hansson, Gunilla Høyer Hansen, and Bertil Casslén. “EGF-Stimulated Migration in Ovarian Cancer Cells Is Associated with Decreased Internalization, Increased Surface Expression, and Increased Shedding of the Urokinase Plasminogen Activator Receptor.” <i>Gynecologic Oncology</i>. Elsevier, 2006. <a href=\"https://doi.org/10.1016/j.ygyno.2005.09.038\">https://doi.org/10.1016/j.ygyno.2005.09.038</a>.","ieee":"E. Henic, M. K. Sixt, S. Hansson, G. Høyer Hansen, and B. Casslén, “EGF-stimulated migration in ovarian cancer cells is associated with decreased internalization, increased surface expression, and increased shedding of the urokinase plasminogen activator receptor,” <i>Gynecologic Oncology</i>, vol. 101, no. 1. Elsevier, pp. 28–39, 2006.","apa":"Henic, E., Sixt, M. K., Hansson, S., Høyer Hansen, G., &#38; Casslén, B. (2006). EGF-stimulated migration in ovarian cancer cells is associated with decreased internalization, increased surface expression, and increased shedding of the urokinase plasminogen activator receptor. <i>Gynecologic Oncology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ygyno.2005.09.038\">https://doi.org/10.1016/j.ygyno.2005.09.038</a>","ista":"Henic E, Sixt MK, Hansson S, Høyer Hansen G, Casslén B. 2006. EGF-stimulated migration in ovarian cancer cells is associated with decreased internalization, increased surface expression, and increased shedding of the urokinase plasminogen activator receptor. Gynecologic Oncology. 101(1), 28–39.","mla":"Henic, Emir, et al. “EGF-Stimulated Migration in Ovarian Cancer Cells Is Associated with Decreased Internalization, Increased Surface Expression, and Increased Shedding of the Urokinase Plasminogen Activator Receptor.” <i>Gynecologic Oncology</i>, vol. 101, no. 1, Elsevier, 2006, pp. 28–39, doi:<a href=\"https://doi.org/10.1016/j.ygyno.2005.09.038\">10.1016/j.ygyno.2005.09.038</a>.","ama":"Henic E, Sixt MK, Hansson S, Høyer Hansen G, Casslén B. EGF-stimulated migration in ovarian cancer cells is associated with decreased internalization, increased surface expression, and increased shedding of the urokinase plasminogen activator receptor. <i>Gynecologic Oncology</i>. 2006;101(1):28-39. doi:<a href=\"https://doi.org/10.1016/j.ygyno.2005.09.038\">10.1016/j.ygyno.2005.09.038</a>","short":"E. Henic, M.K. Sixt, S. Hansson, G. Høyer Hansen, B. Casslén, Gynecologic Oncology 101 (2006) 28–39."},"doi":"10.1016/j.ygyno.2005.09.038","title":"EGF-stimulated migration in ovarian cancer cells is associated with decreased internalization, increased surface expression, and increased shedding of the urokinase plasminogen activator receptor","quality_controlled":0,"issue":"1","type":"journal_article","volume":101,"publication":"Gynecologic Oncology","abstract":[{"text":"OBJECTIVES: The EGFR is expressed in malignant ovarian tumor tissue, and tissue content of EGFR has been directly associated with poor prognosis in patients with ovarian cancer. The uPA system plays a role in pericellular proteolysis, cell migration, invasion, and is over-expressed in ovarian cancer. This study explored the effects of EGF on uPAR expression in the ovarian cancer cell line OVCAR-3. METHODS: We used OVCAR-3 cells and the following methods: cell migration assay, time-lapse video microscopy, real-time PCR, assays for cellular binding of 125I-uPA and cellular degradation of 125I-uPA:PAI-1 complex, biosynthetic labeling using 35S-methionin, Western blot, Northern blot, and ELISAs for uPA, PAI-1, and uPAR. RESULTS: EGF up-regulates both protein and mRNA not only for uPAR, but also for the ligand uPA and its inhibitor PAI-1. Cell surface uPAR, in control as well as EGF-stimulated cells, is present only in the intact, not the cleaved, form. Ligand binding experiments showed an increase of endogenously occupied uPAR, whereas non-occupied receptor sites were not increased. In addition, EGF treatment resulted in decreased degradation of radiolabeled uPA:PAI-1 complex. This suggests decreased internalization of uPAR, since the complex is internalized together with uPAR. Like EGF, colchicine, which inhibits endocytosis, increased cell surface expression of uPAR. In addition, we found an immediate increase of uPAR after exposing the cells to EGF and this was accompanied by a transient increase of cell migration. The increase of cell surface uPAR in response to EGF is accompanied by increased release of the soluble form of uPAR (suPAR) to the medium as well as by increased cell migration. Both uPAR and suPAR increased in cells treated with the endocytosis inhibitor colchicine even though cell migration was inhibited, suggesting that the mechanism of uPAR shedding is not related to cell migration. CONCLUSION: Increased cell surface uPAR in response to EGF stimulation results from mobilization of uPAR from detergent-resistant domains, increased expression of uPAR mRNA, and decreased internalization and degradation of uPAR. Both the anti-uPAR antibody R3, which inhibits binding of uPA, and the EGFR phosphorylation inhibitor Iressa inhibited cell migration in response to uPA as well as to EGF, suggesting that EGFR and uPAR are engaged in the same multiprotein assembly on the cell surface.","lang":"eng"}],"date_updated":"2021-01-12T07:53:17Z","publication_status":"published","page":"28 - 39","date_published":"2006-04-01T00:00:00Z","status":"public","_id":"3932","month":"04","year":"2006","extern":1,"author":[{"full_name":"Henic, Emir","last_name":"Henic","first_name":"Emir"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Michael Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Stefan","last_name":"Hansson","full_name":"Hansson, Stefan"},{"full_name":"Høyer-Hansen, Gunilla","last_name":"Høyer Hansen","first_name":"Gunilla"},{"full_name":"Casslén, Bertil","first_name":"Bertil","last_name":"Casslén"}],"publist_id":"2194","intvolume":"       101","date_created":"2018-12-11T12:05:57Z","day":"01","publisher":"Elsevier"},{"status":"public","month":"04","_id":"3934","year":"2006","extern":1,"author":[{"full_name":"Drumea-Mirancea, Mihaela","first_name":"Mihaela","last_name":"Drumea Mirancea"},{"last_name":"Wessels","first_name":"Johannes","full_name":"Wessels, Johannes T"},{"full_name":"Müller, Claudia A","last_name":"Müller","first_name":"Claudia"},{"full_name":"Essl, Mike","last_name":"Essl","first_name":"Mike"},{"first_name":"Johannes","last_name":"Eble","full_name":"Eble, Johannes A"},{"full_name":"Tolosa, Eva","last_name":"Tolosa","first_name":"Eva"},{"last_name":"Koch","first_name":"Manuel","full_name":"Koch, Manuel"},{"last_name":"Reinhardt","first_name":"Dieter","full_name":"Reinhardt, Dieter P"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Michael Sixt"},{"last_name":"Sorokin","first_name":"Lydia","full_name":"Sorokin, Lydia"},{"full_name":"Stierhof, York-Dieter","first_name":"York","last_name":"Stierhof"},{"last_name":"Schwarz","first_name":"Heinz","full_name":"Schwarz, Heinz"},{"last_name":"Klein","first_name":"Gerd","full_name":"Klein, Gerd"}],"intvolume":"       119","publist_id":"2192","date_created":"2018-12-11T12:05:58Z","publisher":"Company of Biologists","day":"01","publication_status":"published","page":"1396 - 1405","date_published":"2006-04-01T00:00:00Z","type":"journal_article","volume":119,"abstract":[{"text":"T cells develop in the thymus in a highly specialized cellular and extracellular microenvironment. The basement membrane molecule, laminin-5 (LN-5), is predominantly found in the medulla of the human thymic lobules. Using high-resolution light microscopy, we show here that LN-5 is localized in a bi-membranous conduit-like structure, together with other typical basement membrane components including collagen type IV, nidogen and perlecan. Other interstitial matrix components, such as fibrillin-1 or -2, tenascin-C or fibrillar collagen types, were also associated with these structures. Three-dimensional (3D) confocal microscopy suggested a tubular structure, whereas immunoelectron and transmission electron microscopy showed that the core of these tubes contained fibrillar collagens enwrapped by the LN-5-containing membrane. These medullary conduits are surrounded by thymic epithelial cells, which in vitro were found to bind LN-5, but also fibrillin and tenascin-C. Dendritic cells were also detected in close vicinity to the conduits. Both of these stromal cell types express major histocompatibility complex (MHC) class II molecules capable of antigen presentation. The conduits are connected to blood vessels but, with an average diameter of 2 mum, they are too small to transport cells. However, evidence is provided that smaller molecules such as a 10 kDa dextran, but not large molecules (&gt;500 kDa), can be transported in the conduits. These results clearly demonstrate that a conduit system, which is also known from secondary lymphatic organs such as lymph nodes and spleen, is present in the medulla of the human thymus, and that it might serve to transport small blood-borne molecules or chemokines to defined locations within the medulla.","lang":"eng"}],"publication":"Journal of Cell Science","date_updated":"2021-01-12T07:53:18Z","doi":"10.1242/​jcs.02840","citation":{"mla":"Drumea Mirancea, Mihaela, et al. “Characterization of a Conduit System Containing Laminin-5 in the Human Thymus: A Potential Transport System for Small Molecules.” <i>Journal of Cell Science</i>, vol. 119, no. Pt 7, Company of Biologists, 2006, pp. 1396–405, doi:<a href=\"https://doi.org/10.1242/​jcs.02840\">10.1242/​jcs.02840</a>.","ama":"Drumea Mirancea M, Wessels J, Müller C, et al. Characterization of a conduit system containing laminin-5 in the human thymus: a potential transport system for small molecules. <i>Journal of Cell Science</i>. 2006;119(Pt 7):1396-1405. doi:<a href=\"https://doi.org/10.1242/​jcs.02840\">10.1242/​jcs.02840</a>","short":"M. Drumea Mirancea, J. Wessels, C. Müller, M. Essl, J. Eble, E. Tolosa, M. Koch, D. Reinhardt, M.K. Sixt, L. Sorokin, Y. Stierhof, H. Schwarz, G. Klein, Journal of Cell Science 119 (2006) 1396–1405.","chicago":"Drumea Mirancea, Mihaela, Johannes Wessels, Claudia Müller, Mike Essl, Johannes Eble, Eva Tolosa, Manuel Koch, et al. “Characterization of a Conduit System Containing Laminin-5 in the Human Thymus: A Potential Transport System for Small Molecules.” <i>Journal of Cell Science</i>. Company of Biologists, 2006. <a href=\"https://doi.org/10.1242/​jcs.02840\">https://doi.org/10.1242/​jcs.02840</a>.","ieee":"M. Drumea Mirancea <i>et al.</i>, “Characterization of a conduit system containing laminin-5 in the human thymus: a potential transport system for small molecules,” <i>Journal of Cell Science</i>, vol. 119, no. Pt 7. Company of Biologists, pp. 1396–1405, 2006.","apa":"Drumea Mirancea, M., Wessels, J., Müller, C., Essl, M., Eble, J., Tolosa, E., … Klein, G. (2006). Characterization of a conduit system containing laminin-5 in the human thymus: a potential transport system for small molecules. <i>Journal of Cell Science</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/​jcs.02840\">https://doi.org/10.1242/​jcs.02840</a>","ista":"Drumea Mirancea M, Wessels J, Müller C, Essl M, Eble J, Tolosa E, Koch M, Reinhardt D, Sixt MK, Sorokin L, Stierhof Y, Schwarz H, Klein G. 2006. Characterization of a conduit system containing laminin-5 in the human thymus: a potential transport system for small molecules. Journal of Cell Science. 119(Pt 7), 1396–1405."},"title":"Characterization of a conduit system containing laminin-5 in the human thymus: a potential transport system for small molecules","quality_controlled":0,"issue":"Pt 7"},{"type":"journal_article","volume":26,"abstract":[{"lang":"eng","text":"Integrins regulate cell behavior through the assembly of multiprotein complexes at the site of cell adhesion. Parvins are components of such a multiprotein complex. They consist of three members (alpha-, beta-, and gamma-parvin), form a functional complex with integrin-linked kinase (ILK) and PINCH, and link integrins to the actin cytoskeleton. Whereas alpha- and beta-parvins are widely expressed, gamma-parvin has been reported to be expressed in hematopoietic organs. In the present study, we report the expression pattern of the parvins in hematopoietic cells and the phenotypic analysis of gamma-parvin-deficient mice. Whereas alpha-parvin is not expressed in hematopoietic cells, beta-parvin is only found in myeloid cells and gamma-parvin is present in both cells of the myeloid and lymphoid lineages, where it binds ILK. Surprisingly, loss of gamma-parvin expression had no effect on blood cell differentiation, proliferation, and survival and no consequence for the T-cell-dependent antibody response and lymphocyte and dendritic cell migration. These data indicate that despite the high expression of gamma-parvin in hematopoietic cells it must play a more subtle role for blood cell homeostasis."}],"publication":"Molecular and Cellular Biology","date_updated":"2021-01-12T07:53:18Z","citation":{"apa":"Chu, H., Thievessen, I., Sixt, M. K., Lämmermann, T., Waisman, A., Braun, A., … Fässler, R. (2006). γ-Parvin is dispensable for hematopoiesis, leukocyte trafficking, and T-cell-dependent antibody response. <i>Molecular and Cellular Biology</i>. American Society for Microbiology. <a href=\"https://doi.org/10.1128/MCB.26.5.1817-1825.2006\">https://doi.org/10.1128/MCB.26.5.1817-1825.2006</a>","chicago":"Chu, Haiyan, Ingo Thievessen, Michael K Sixt, Tim Lämmermann, Ari Waisman, Attila Braun, Angelika Noegel, and Reinhard Fässler. “γ-Parvin Is Dispensable for Hematopoiesis, Leukocyte Trafficking, and T-Cell-Dependent Antibody Response.” <i>Molecular and Cellular Biology</i>. American Society for Microbiology, 2006. <a href=\"https://doi.org/10.1128/MCB.26.5.1817-1825.2006\">https://doi.org/10.1128/MCB.26.5.1817-1825.2006</a>.","ieee":"H. Chu <i>et al.</i>, “γ-Parvin is dispensable for hematopoiesis, leukocyte trafficking, and T-cell-dependent antibody response,” <i>Molecular and Cellular Biology</i>, vol. 26, no. 5. American Society for Microbiology, pp. 1817–1825, 2006.","ista":"Chu H, Thievessen I, Sixt MK, Lämmermann T, Waisman A, Braun A, Noegel A, Fässler R. 2006. γ-Parvin is dispensable for hematopoiesis, leukocyte trafficking, and T-cell-dependent antibody response. Molecular and Cellular Biology. 26(5), 1817–1825.","mla":"Chu, Haiyan, et al. “γ-Parvin Is Dispensable for Hematopoiesis, Leukocyte Trafficking, and T-Cell-Dependent Antibody Response.” <i>Molecular and Cellular Biology</i>, vol. 26, no. 5, American Society for Microbiology, 2006, pp. 1817–25, doi:<a href=\"https://doi.org/10.1128/MCB.26.5.1817-1825.2006\">10.1128/MCB.26.5.1817-1825.2006</a>.","short":"H. Chu, I. Thievessen, M.K. Sixt, T. Lämmermann, A. Waisman, A. Braun, A. Noegel, R. Fässler, Molecular and Cellular Biology 26 (2006) 1817–1825.","ama":"Chu H, Thievessen I, Sixt MK, et al. γ-Parvin is dispensable for hematopoiesis, leukocyte trafficking, and T-cell-dependent antibody response. <i>Molecular and Cellular Biology</i>. 2006;26(5):1817-1825. doi:<a href=\"https://doi.org/10.1128/MCB.26.5.1817-1825.2006\">10.1128/MCB.26.5.1817-1825.2006</a>"},"doi":"10.1128/MCB.26.5.1817-1825.2006","quality_controlled":0,"title":"γ-Parvin is dispensable for hematopoiesis, leukocyte trafficking, and T-cell-dependent antibody response","issue":"5","month":"03","_id":"3935","status":"public","year":"2006","publist_id":"2193","intvolume":"        26","author":[{"full_name":"Chu, Haiyan","first_name":"Haiyan","last_name":"Chu"},{"last_name":"Thievessen","first_name":"Ingo","full_name":"Thievessen, Ingo"},{"orcid":"0000-0002-6620-9179","full_name":"Michael Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt"},{"full_name":"Lämmermann, Tim","last_name":"Lämmermann","first_name":"Tim"},{"last_name":"Waisman","first_name":"Ari","full_name":"Waisman, Ari"},{"full_name":"Braun, Attila","first_name":"Attila","last_name":"Braun"},{"full_name":"Noegel, Angelika A","last_name":"Noegel","first_name":"Angelika"},{"full_name":"Fässler, Reinhard","first_name":"Reinhard","last_name":"Fässler"}],"extern":1,"day":"01","publisher":"American Society for Microbiology","date_created":"2018-12-11T12:05:58Z","publication_status":"published","page":"1817 - 1825","date_published":"2006-03-01T00:00:00Z"},{"date_updated":"2021-01-12T07:53:19Z","publication":"Current Opinion in Cell Biology","abstract":[{"text":"At least eight of the twelve known members of the beta1 integrin family are expressed on hematopoietic cells. Among these, the VCAM-1 receptor alpha4beta1 has received most attention as a main factor mediating firm adhesion to the endothelium during blood cell extravasation. Therapeutic trials are ongoing into the use of antibodies and small molecule inhibitors to target this interaction and hence obtain anti-inflammatory effects. However, extravasation is only one possible process that is mediated by beta1 integrins and there is evidence that they also mediate leukocyte retention and positioning in the tissue, lymphocyte activation and possibly migration within the interstitium. Genetic mouse models where integrins are selectively deleted on blood cells have been used to investigate these functions and further studies will be invaluable to critically evaluate therapeutic trials.","lang":"eng"}],"type":"journal_article","volume":18,"issue":"5","title":"β1 integrins: zip codes and signaling relay for blood cells","quality_controlled":0,"citation":{"mla":"Sixt, Michael K., et al. “Β1 Integrins: Zip Codes and Signaling Relay for Blood Cells.” <i>Current Opinion in Cell Biology</i>, vol. 18, no. 5, Elsevier, 2006, pp. 482–90, doi:<a href=\"https://doi.org/10.1016/j.ceb.2006.08.007\">10.1016/j.ceb.2006.08.007</a>.","ama":"Sixt MK, Bauer M, Lämmermann T, Fässler R. β1 integrins: zip codes and signaling relay for blood cells. <i>Current Opinion in Cell Biology</i>. 2006;18(5):482-490. doi:<a href=\"https://doi.org/10.1016/j.ceb.2006.08.007\">10.1016/j.ceb.2006.08.007</a>","short":"M.K. Sixt, M. Bauer, T. Lämmermann, R. Fässler, Current Opinion in Cell Biology 18 (2006) 482–490.","chicago":"Sixt, Michael K, Martina Bauer, Tim Lämmermann, and Reinhard Fässler. “Β1 Integrins: Zip Codes and Signaling Relay for Blood Cells.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2006. <a href=\"https://doi.org/10.1016/j.ceb.2006.08.007\">https://doi.org/10.1016/j.ceb.2006.08.007</a>.","ieee":"M. K. Sixt, M. Bauer, T. Lämmermann, and R. Fässler, “β1 integrins: zip codes and signaling relay for blood cells,” <i>Current Opinion in Cell Biology</i>, vol. 18, no. 5. Elsevier, pp. 482–490, 2006.","apa":"Sixt, M. K., Bauer, M., Lämmermann, T., &#38; Fässler, R. (2006). β1 integrins: zip codes and signaling relay for blood cells. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2006.08.007\">https://doi.org/10.1016/j.ceb.2006.08.007</a>","ista":"Sixt MK, Bauer M, Lämmermann T, Fässler R. 2006. β1 integrins: zip codes and signaling relay for blood cells. Current Opinion in Cell Biology. 18(5), 482–490."},"doi":"10.1016/j.ceb.2006.08.007","date_created":"2018-12-11T12:05:59Z","day":"01","publisher":"Elsevier","author":[{"last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Michael Sixt","orcid":"0000-0002-6620-9179"},{"full_name":"Bauer, Martina","first_name":"Martina","last_name":"Bauer"},{"full_name":"Lämmermann, Tim","first_name":"Tim","last_name":"Lämmermann"},{"first_name":"Reinhard","last_name":"Fässler","full_name":"Fässler, Reinhard"}],"extern":1,"publist_id":"2191","intvolume":"        18","year":"2006","status":"public","_id":"3936","month":"10","date_published":"2006-10-01T00:00:00Z","page":"482 - 490","publication_status":"published"},{"publication_status":"published","page":"852 - 864","date_published":"2006-03-01T00:00:00Z","month":"03","_id":"3978","status":"public","year":"2006","intvolume":"        62","publist_id":"2146","extern":1,"author":[{"full_name":"Ban, Yih-En Andrew","last_name":"Ban","first_name":"Yih"},{"last_name":"Rudolph","first_name":"Johannes","full_name":"Rudolph, Johannes"},{"full_name":"Zhou, Pei","first_name":"Pei","last_name":"Zhou"},{"first_name":"Herbert","last_name":"Edelsbrunner","orcid":"0000-0002-9823-6833","id":"3FB178DA-F248-11E8-B48F-1D18A9856A87","full_name":"Herbert Edelsbrunner"}],"day":"01","publisher":"Wiley-Blackwell","date_created":"2018-12-11T12:06:14Z","citation":{"ama":"Ban Y, Rudolph J, Zhou P, Edelsbrunner H. Evaluating the quality of NMR structures by local density of protons. <i>Proteins: Structure, Function and Bioinformatics</i>. 2006;62(4):852-864. doi:<a href=\"https://doi.org/10.1002/prot.20811\">10.1002/prot.20811</a>","short":"Y. Ban, J. Rudolph, P. Zhou, H. Edelsbrunner, Proteins: Structure, Function and Bioinformatics 62 (2006) 852–864.","mla":"Ban, Yih, et al. “Evaluating the Quality of NMR Structures by Local Density of Protons.” <i>Proteins: Structure, Function and Bioinformatics</i>, vol. 62, no. 4, Wiley-Blackwell, 2006, pp. 852–64, doi:<a href=\"https://doi.org/10.1002/prot.20811\">10.1002/prot.20811</a>.","ista":"Ban Y, Rudolph J, Zhou P, Edelsbrunner H. 2006. Evaluating the quality of NMR structures by local density of protons. Proteins: Structure, Function and Bioinformatics. 62(4), 852–864.","chicago":"Ban, Yih, Johannes Rudolph, Pei Zhou, and Herbert Edelsbrunner. “Evaluating the Quality of NMR Structures by Local Density of Protons.” <i>Proteins: Structure, Function and Bioinformatics</i>. Wiley-Blackwell, 2006. <a href=\"https://doi.org/10.1002/prot.20811\">https://doi.org/10.1002/prot.20811</a>.","ieee":"Y. Ban, J. Rudolph, P. Zhou, and H. Edelsbrunner, “Evaluating the quality of NMR structures by local density of protons,” <i>Proteins: Structure, Function and Bioinformatics</i>, vol. 62, no. 4. Wiley-Blackwell, pp. 852–864, 2006.","apa":"Ban, Y., Rudolph, J., Zhou, P., &#38; Edelsbrunner, H. (2006). Evaluating the quality of NMR structures by local density of protons. <i>Proteins: Structure, Function and Bioinformatics</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/prot.20811\">https://doi.org/10.1002/prot.20811</a>"},"doi":"10.1002/prot.20811","quality_controlled":0,"title":"Evaluating the quality of NMR structures by local density of protons","issue":"4","type":"journal_article","volume":62,"abstract":[{"lang":"eng","text":"Evaluating the quality of experimentally determined protein structural models is an essential step toward identifying potential errors and guiding further structural refinement. Herein, we report the use of proton local density as a sensitive measure to assess the quality of nuclear magnetic resonance (NMR) structures. Using 256 high-resolution crystal structures with protons added and optimized, we show that the local density of different proton types display distinct distributions. These distributions can be characterized by statistical moments and are used to establish local density Z-scores for evaluating both global and local packing for individual protons. Analysis of 546 crystal structures at various resolutions shows that the local density Z-scores increase as the structural resolution decreases and correlate well with the ClashScore (Word et al. J Mol Biol 1999;285(4):1711-1733) generated by all atom contact analysis. Local density Z-scores for NMR structures exhibit a significantly wider range of values than for X-ray structures and demonstrate a combination of potentially problematic inflation and compression. Water-refined NMR structures show improved packing quality. Our analysis of a high-quality structural ensemble of ubiquitin refined against order parameters shows proton density distributions that correlate nearly perfectly with our standards derived from crystal structures, further validating our approach. We present an automated analysis and visualization tool for proton packing to evaluate the quality of NMR structures."}],"publication":"Proteins: Structure, Function and Bioinformatics","date_updated":"2021-01-12T07:53:36Z"},{"abstract":[{"lang":"eng","text":"Protein-protein interactions, which form the basis for most cellular processes, result in the formation of protein interfaces. Believing that the local shape of proteins is crucial, we take a geometric approach and present a definition of an interface surface formed by two or more proteins as a subset of their Voronoi diagram. The definition deals with the difficult and important problem of specifying interface boundaries by invoking methods used in the alpha shape representation of molecules, the discrete flow on Delaunay simplices to define pockets and reconstruct surfaces, and the assessment of the importance of topological features. We present an algorithm to construct the surface and define a hierarchy that distinguishes core and peripheral regions. This hierarchy is shown to have correlation with hot-spots in protein-protein interactions. Finally, we study the geometric and topological properties of interface surfaces and show their high degree of contortion."}],"publication":"Journal of the ACM","date_updated":"2021-01-12T07:53:37Z","type":"journal_article","volume":53,"title":"Interface surfaces for protein-protein complexes","quality_controlled":0,"issue":"3","citation":{"apa":"Ban, Y., Edelsbrunner, H., &#38; Rudolph, J. (2006). Interface surfaces for protein-protein complexes. <i>Journal of the ACM</i>. ACM. <a href=\"https://doi.org/10.1145/1147954.1147957\">https://doi.org/10.1145/1147954.1147957</a>","ieee":"Y. Ban, H. Edelsbrunner, and J. Rudolph, “Interface surfaces for protein-protein complexes,” <i>Journal of the ACM</i>, vol. 53, no. 3. ACM, pp. 361–378, 2006.","chicago":"Ban, Yih, Herbert Edelsbrunner, and Johannes Rudolph. “Interface Surfaces for Protein-Protein Complexes.” <i>Journal of the ACM</i>. ACM, 2006. <a href=\"https://doi.org/10.1145/1147954.1147957\">https://doi.org/10.1145/1147954.1147957</a>.","ista":"Ban Y, Edelsbrunner H, Rudolph J. 2006. Interface surfaces for protein-protein complexes. Journal of the ACM. 53(3), 361–378.","mla":"Ban, Yih, et al. “Interface Surfaces for Protein-Protein Complexes.” <i>Journal of the ACM</i>, vol. 53, no. 3, ACM, 2006, pp. 361–78, doi:<a href=\"https://doi.org/10.1145/1147954.1147957\">10.1145/1147954.1147957</a>.","short":"Y. Ban, H. Edelsbrunner, J. Rudolph, Journal of the ACM 53 (2006) 361–378.","ama":"Ban Y, Edelsbrunner H, Rudolph J. Interface surfaces for protein-protein complexes. <i>Journal of the ACM</i>. 2006;53(3):361-378. doi:<a href=\"https://doi.org/10.1145/1147954.1147957\">10.1145/1147954.1147957</a>"},"doi":"10.1145/1147954.1147957","extern":1,"author":[{"full_name":"Ban, Yih-En Andrew","first_name":"Yih","last_name":"Ban"},{"last_name":"Edelsbrunner","first_name":"Herbert","id":"3FB178DA-F248-11E8-B48F-1D18A9856A87","full_name":"Herbert Edelsbrunner","orcid":"0000-0002-9823-6833"},{"first_name":"Johannes","last_name":"Rudolph","full_name":"Rudolph, Johannes"}],"intvolume":"        53","publist_id":"2147","date_created":"2018-12-11T12:06:14Z","day":"01","publisher":"ACM","status":"public","month":"05","_id":"3979","year":"2006","date_published":"2006-05-01T00:00:00Z","page":"361 - 378","publication_status":"published"},{"year":"2006","status":"public","_id":"3980","month":"12","date_created":"2018-12-11T12:06:15Z","publisher":"Springer","day":"01","author":[{"full_name":"Agarwal, Pankaj K","first_name":"Pankaj","last_name":"Agarwal"},{"full_name":"Herbert Edelsbrunner","id":"3FB178DA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9823-6833","last_name":"Edelsbrunner","first_name":"Herbert"},{"full_name":"Harer, John","first_name":"John","last_name":"Harer"},{"last_name":"Wang","first_name":"Yusu","full_name":"Wang, Yusu"}],"extern":1,"publist_id":"2148","intvolume":"        36","page":"553 - 572","publication_status":"published","date_published":"2006-12-01T00:00:00Z","volume":36,"type":"journal_article","date_updated":"2021-01-12T07:53:38Z","abstract":[{"text":"Given a smoothly embedded 2-manifold in R-3, we define the elevation of a point as the height difference to a canonically defined second point on the same manifold. Our definition is invariant under rigid motions and can be used to define features such as lines of discontinuous or continuous but non-smooth elevation. We give an algorithm for finding points of locally maximum elevation, which we suggest mark cavities and protrusions and are useful in matching shapes as for example in protein docking.","lang":"eng"}],"publication":"Discrete & Computational Geometry","citation":{"ista":"Agarwal P, Edelsbrunner H, Harer J, Wang Y. 2006. Extreme elevation on a 2-manifold. Discrete &#38; Computational Geometry. 36(4), 553–572.","apa":"Agarwal, P., Edelsbrunner, H., Harer, J., &#38; Wang, Y. (2006). Extreme elevation on a 2-manifold. <i>Discrete &#38; Computational Geometry</i>. Springer. <a href=\"https://doi.org/10.1007/s00454-006-1265-8\">https://doi.org/10.1007/s00454-006-1265-8</a>","ieee":"P. Agarwal, H. Edelsbrunner, J. Harer, and Y. Wang, “Extreme elevation on a 2-manifold,” <i>Discrete &#38; Computational Geometry</i>, vol. 36, no. 4. Springer, pp. 553–572, 2006.","chicago":"Agarwal, Pankaj, Herbert Edelsbrunner, John Harer, and Yusu Wang. “Extreme Elevation on a 2-Manifold.” <i>Discrete &#38; Computational Geometry</i>. Springer, 2006. <a href=\"https://doi.org/10.1007/s00454-006-1265-8\">https://doi.org/10.1007/s00454-006-1265-8</a>.","short":"P. Agarwal, H. Edelsbrunner, J. Harer, Y. Wang, Discrete &#38; Computational Geometry 36 (2006) 553–572.","ama":"Agarwal P, Edelsbrunner H, Harer J, Wang Y. Extreme elevation on a 2-manifold. <i>Discrete &#38; Computational Geometry</i>. 2006;36(4):553-572. doi:<a href=\"https://doi.org/10.1007/s00454-006-1265-8\">10.1007/s00454-006-1265-8</a>","mla":"Agarwal, Pankaj, et al. “Extreme Elevation on a 2-Manifold.” <i>Discrete &#38; Computational Geometry</i>, vol. 36, no. 4, Springer, 2006, pp. 553–72, doi:<a href=\"https://doi.org/10.1007/s00454-006-1265-8\">10.1007/s00454-006-1265-8</a>."},"doi":"10.1007/s00454-006-1265-8","issue":"4","title":"Extreme elevation on a 2-manifold","quality_controlled":0},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":175,"type":"journal_article","article_processing_charge":"No","date_updated":"2021-01-12T07:54:48Z","publication":"Journal of Cell Biology","abstract":[{"lang":"eng","text":"Wnt11 is a key signal, determining cell polarization and migration during vertebrate gastrulation. It is known that Wnt11 functionally interacts with several signaling components, the homologues of which control planar cell polarity in Drosophila melanogaster. Although in D. melanogaster these components are thought to polarize cells by asymmetrically localizing at the plasma membrane, it is not yet clear whether their subcellular localization plays a similarly important role in vertebrates. We show that in zebrafish embryonic cells, Wnt11 locally functions at the plasma membrane by accumulating its receptor, Frizzled 7, on adjacent sites of cell contacts. Wnt11-induced Frizzled 7 accumulations recruit the intracellular Wnt signaling mediator Dishevelled, as well as Wnt11 itself, and locally increase cell contact persistence. This increase in cell contact persistence is mediated by the local interaction of Wnt11, Frizzled 7, and the atypical cadherin Flamingo at the plasma membrane, and it does not require the activity of further downstream effectors of Wnt11 signaling, such as RhoA and Rok2. We propose that Wnt11, by interacting with Frizzled 7 and Flamingo, modulates local cell contact persistence to coordinate cell movements during gastrulation."}],"language":[{"iso":"eng"}],"doi":"10.1083/jcb.200606017","citation":{"mla":"Witzel, Sabine, et al. “Wnt11 Controls Cell Contact Persistence by Local Accumulation of Frizzled 7 at the Plasma Membrane.” <i>Journal of Cell Biology</i>, vol. 175, no. 5, Rockefeller University Press, 2006, pp. 791–802, doi:<a href=\"https://doi.org/10.1083/jcb.200606017\">10.1083/jcb.200606017</a>.","ama":"Witzel S, Zimyanin V, Carreira Barbosa F, Tada M, Heisenberg C-PJ. Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane. <i>Journal of Cell Biology</i>. 2006;175(5):791-802. doi:<a href=\"https://doi.org/10.1083/jcb.200606017\">10.1083/jcb.200606017</a>","short":"S. Witzel, V. Zimyanin, F. Carreira Barbosa, M. Tada, C.-P.J. Heisenberg, Journal of Cell Biology 175 (2006) 791–802.","chicago":"Witzel, Sabine, Vitaly Zimyanin, Filipa Carreira Barbosa, Masazumi Tada, and Carl-Philipp J Heisenberg. “Wnt11 Controls Cell Contact Persistence by Local Accumulation of Frizzled 7 at the Plasma Membrane.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2006. <a href=\"https://doi.org/10.1083/jcb.200606017\">https://doi.org/10.1083/jcb.200606017</a>.","ieee":"S. Witzel, V. Zimyanin, F. Carreira Barbosa, M. Tada, and C.-P. J. Heisenberg, “Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane,” <i>Journal of Cell Biology</i>, vol. 175, no. 5. Rockefeller University Press, pp. 791–802, 2006.","apa":"Witzel, S., Zimyanin, V., Carreira Barbosa, F., Tada, M., &#38; Heisenberg, C.-P. J. (2006). Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.200606017\">https://doi.org/10.1083/jcb.200606017</a>","ista":"Witzel S, Zimyanin V, Carreira Barbosa F, Tada M, Heisenberg C-PJ. 2006. Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane. Journal of Cell Biology. 175(5), 791–802."},"oa_version":"None","issue":"5","title":"Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane","year":"2006","status":"public","month":"12","_id":"4140","date_created":"2018-12-11T12:07:11Z","day":"04","publisher":"Rockefeller University Press","author":[{"first_name":"Sabine","last_name":"Witzel","full_name":"Witzel, Sabine"},{"first_name":"Vitaly","last_name":"Zimyanin","full_name":"Zimyanin, Vitaly"},{"first_name":"Filipa","last_name":"Carreira Barbosa","full_name":"Carreira Barbosa, Filipa"},{"full_name":"Tada, Masazumi","last_name":"Tada","first_name":"Masazumi"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"extern":"1","intvolume":"       175","publist_id":"1980","publication_status":"published","page":"791 - 802","date_published":"2006-12-04T00:00:00Z"},{"date_updated":"2021-01-12T07:54:50Z","language":[{"iso":"eng"}],"abstract":[{"text":"The detection of microRNAs (miRNAs) at single-cell resolution is important for studying the role of these posttranscriptional regulators. Here, we use a dual-fluorescent green fluorescent protein (GFP)-reporter/monomeric red fluorescent protein (mRFP)-sensor (DFRS) plasmid, injected into zebrafish blastomeres or electroporated into defined tissues of mouse embryos in utero or ex utero, to monitor the dynamics of specific miRNAs in individual live cells. This approach reveals, for example, that in the developing mouse central nervous system,, miR-124a is expressed not only in postmitotic neurons but also in neuronal progenitor cells. Collectively, our results demonstrate that acute administration of DFRS plasmids.offers an alternative to previous in situ hybridization and transgenic approaches and allows the monitoring of miRNA appearance and disappearance in defined cell lineages during vertebrate development.","lang":"eng"}],"publication":"Biotechniques","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":41,"article_processing_charge":"No","type":"journal_article","issue":"6","title":"Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid","citation":{"short":"D. Tonelli, F. Calegari, J. Fei, T. Nomura, N. Osumi, C.-P.J. Heisenberg, W. Huttner, Biotechniques 41 (2006) 727–732.","ama":"Tonelli D, Calegari F, Fei J, et al. Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid. <i>Biotechniques</i>. 2006;41(6):727-732. doi:<a href=\"https://doi.org/10.2144/000112296\">10.2144/000112296</a>","mla":"Tonelli, Davide, et al. “Single-Cell Detection of MicroRNAs in Developing Vertebrate Embryos after Acute Administration of a Dual-Fluorescence Reporter/Sensor Plasmid.” <i>Biotechniques</i>, vol. 41, no. 6, Informa Healthcare, 2006, pp. 727–32, doi:<a href=\"https://doi.org/10.2144/000112296\">10.2144/000112296</a>.","ista":"Tonelli D, Calegari F, Fei J, Nomura T, Osumi N, Heisenberg C-PJ, Huttner W. 2006. Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid. Biotechniques. 41(6), 727–732.","apa":"Tonelli, D., Calegari, F., Fei, J., Nomura, T., Osumi, N., Heisenberg, C.-P. J., &#38; Huttner, W. (2006). Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid. <i>Biotechniques</i>. Informa Healthcare. <a href=\"https://doi.org/10.2144/000112296\">https://doi.org/10.2144/000112296</a>","chicago":"Tonelli, Davide, Frederico Calegari, Ji Fei, Tadashi Nomura, Noriko Osumi, Carl-Philipp J Heisenberg, and Wieland Huttner. “Single-Cell Detection of MicroRNAs in Developing Vertebrate Embryos after Acute Administration of a Dual-Fluorescence Reporter/Sensor Plasmid.” <i>Biotechniques</i>. Informa Healthcare, 2006. <a href=\"https://doi.org/10.2144/000112296\">https://doi.org/10.2144/000112296</a>.","ieee":"D. Tonelli <i>et al.</i>, “Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid,” <i>Biotechniques</i>, vol. 41, no. 6. Informa Healthcare, pp. 727–732, 2006."},"doi":"10.2144/000112296","oa_version":"None","day":"01","publisher":"Informa Healthcare","date_created":"2018-12-11T12:07:12Z","intvolume":"        41","publist_id":"1974","extern":"1","author":[{"first_name":"Davide","last_name":"Tonelli","full_name":"Tonelli, Davide"},{"full_name":"Calegari, Frederico","first_name":"Frederico","last_name":"Calegari"},{"full_name":"Fei, Ji","last_name":"Fei","first_name":"Ji"},{"full_name":"Nomura, Tadashi","last_name":"Nomura","first_name":"Tadashi"},{"last_name":"Osumi","first_name":"Noriko","full_name":"Osumi, Noriko"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"last_name":"Huttner","first_name":"Wieland","full_name":"Huttner, Wieland"}],"year":"2006","month":"12","_id":"4145","status":"public","date_published":"2006-12-01T00:00:00Z","page":"727 - 732","publication_status":"published"},{"status":"public","_id":"4173","month":"01","year":"2006","author":[{"first_name":"Vinzenz","last_name":"Link","full_name":"Link, Vinzenz"},{"first_name":"Andrej","last_name":"Shevchenko","full_name":"Shevchenko, Andrej"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"extern":"1","publist_id":"1945","intvolume":"         6","date_created":"2018-12-11T12:07:23Z","publisher":"BioMed Central","day":"13","page":"1 - 9","publication_status":"published","date_published":"2006-01-13T00:00:00Z","main_file_link":[{"open_access":"1","url":"http://www.biomedcentral.com/1471-213X/6/1"}],"article_processing_charge":"No","volume":6,"type":"journal_article","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"BMC Developmental Biology","abstract":[{"text":"Background: Zebrafish (D. rerio) has become a powerful and widely used model system for the analysis of vertebrate embryogenesis and organ development. While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed. Results: As a prerequisite to carry out proteomic experiments with early zebrafish embryos, we developed a method to efficiently remove the yolk from large batches of embryos. This method enabled high resolution 2D gel electrophoresis and improved Western blotting considerably. Here, we provide detailed protocols for proteomics in zebrafish from sample preparation to mass spectrometry (MS), including a comparison of databases for MS identification of zebrafish proteins. Conclusion: The provided protocols for proteomic analysis of early embryos enable research to be taken in novel directions in embryogenesis.","lang":"eng"}],"language":[{"iso":"eng"}],"date_updated":"2021-01-12T07:55:02Z","doi":"10.1186/1471-213X-6-1","citation":{"ama":"Link V, Shevchenko A, Heisenberg C-PJ. Proteomics of early zebrafish embryos. <i>BMC Developmental Biology</i>. 2006;6:1-9. doi:<a href=\"https://doi.org/10.1186/1471-213X-6-1\">10.1186/1471-213X-6-1</a>","short":"V. Link, A. Shevchenko, C.-P.J. Heisenberg, BMC Developmental Biology 6 (2006) 1–9.","mla":"Link, Vinzenz, et al. “Proteomics of Early Zebrafish Embryos.” <i>BMC Developmental Biology</i>, vol. 6, BioMed Central, 2006, pp. 1–9, doi:<a href=\"https://doi.org/10.1186/1471-213X-6-1\">10.1186/1471-213X-6-1</a>.","ista":"Link V, Shevchenko A, Heisenberg C-PJ. 2006. Proteomics of early zebrafish embryos. BMC Developmental Biology. 6, 1–9.","ieee":"V. Link, A. Shevchenko, and C.-P. J. Heisenberg, “Proteomics of early zebrafish embryos,” <i>BMC Developmental Biology</i>, vol. 6. BioMed Central, pp. 1–9, 2006.","chicago":"Link, Vinzenz, Andrej Shevchenko, and Carl-Philipp J Heisenberg. “Proteomics of Early Zebrafish Embryos.” <i>BMC Developmental Biology</i>. BioMed Central, 2006. <a href=\"https://doi.org/10.1186/1471-213X-6-1\">https://doi.org/10.1186/1471-213X-6-1</a>.","apa":"Link, V., Shevchenko, A., &#38; Heisenberg, C.-P. J. (2006). Proteomics of early zebrafish embryos. <i>BMC Developmental Biology</i>. BioMed Central. <a href=\"https://doi.org/10.1186/1471-213X-6-1\">https://doi.org/10.1186/1471-213X-6-1</a>"},"oa_version":"None","title":"Proteomics of early zebrafish embryos","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}}]