[{"issue":"1","article_type":"original","type":"journal_article","article_processing_charge":"No","doi":"10.1111/gcb.16995","day":"01","_id":"22511","keyword":["Aerodynamic effects","Bushfires Australia","Ecohydrological modeling","Eddy covariancemeasurements","Eucalyptus regnans","Forest evapotranspiration","Forest recovery","Mountain Ash","TERN OzFlux","Wildfires"],"fulldoi":"https://doi.org/10.1111/gcb.16995","language":[{"iso":"eng"}],"oa_version":"Published Version","citation":{"ista":"Meili N, Beringer J, Zhao J, Fatichi S. 2024. Aerodynamic effects cause higher forest evapotranspiration and water yield reductions after wildfires in tall forests. Global Change Biology. 30(1), e16995.","chicago":"Meili, Naika, Jason Beringer, Jiacheng Zhao, and Simone Fatichi. “Aerodynamic Effects Cause Higher Forest Evapotranspiration and Water Yield Reductions after Wildfires in Tall Forests.” <i>Global Change Biology</i>. Wiley, 2024. <a href=\"https://doi.org/10.1111/gcb.16995\">https://doi.org/10.1111/gcb.16995</a>.","mla":"Meili, Naika, et al. “Aerodynamic Effects Cause Higher Forest Evapotranspiration and Water Yield Reductions after Wildfires in Tall Forests.” <i>Global Change Biology</i>, vol. 30, no. 1, e16995, Wiley, 2024, doi:<a href=\"https://doi.org/10.1111/gcb.16995\">10.1111/gcb.16995</a>.","ieee":"N. Meili, J. Beringer, J. Zhao, and S. Fatichi, “Aerodynamic effects cause higher forest evapotranspiration and water yield reductions after wildfires in tall forests,” <i>Global Change Biology</i>, vol. 30, no. 1. Wiley, 2024.","ama":"Meili N, Beringer J, Zhao J, Fatichi S. Aerodynamic effects cause higher forest evapotranspiration and water yield reductions after wildfires in tall forests. <i>Global Change Biology</i>. 2024;30(1). doi:<a href=\"https://doi.org/10.1111/gcb.16995\">10.1111/gcb.16995</a>","short":"N. Meili, J. Beringer, J. Zhao, S. Fatichi, Global Change Biology 30 (2024).","apa":"Meili, N., Beringer, J., Zhao, J., &#38; Fatichi, S. (2024). Aerodynamic effects cause higher forest evapotranspiration and water yield reductions after wildfires in tall forests. <i>Global Change Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/gcb.16995\">https://doi.org/10.1111/gcb.16995</a>"},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/gcb.16995"}],"extern":"1","article_number":"e16995","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","external_id":{"pmid":["37916642"]},"publication_status":"published","publication_identifier":{"issn":["1354-1013"],"eissn":["1365-2486"]},"title":"Aerodynamic effects cause higher forest evapotranspiration and water yield reductions after wildfires in tall forests","publisher":"Wiley","abstract":[{"lang":"eng","text":"Wildfires are increasing in frequency, intensity, and extent globally due to climate change and they can alter forest composition, structure, and function. The destruction and subsequent regrowth of young vegetation can modify the ecosystem evapotranspiration and downstream water availability. However, the response of forest recovery on hydrology is not well known with even the sign of evapotranspiration and water yield changes following forest fires being uncertain across the globe. Here, we quantify the effects of forest regrowth after catastrophic wildfires on evapotranspiration and runoff in the world's tallest angiosperm forest (Eucalyptus regnans) in Australia. We combine eddy covariance measurements including pre- and post-fire periods, mechanistic ecohydrological modeling and then extend the analysis spatially to multiple fires in eucalypt-dominated forests in south-eastern Australia by utilizing remote sensing. We find a fast recovery of evapotranspiration which reaches and exceeds pre-fire values within 2 years after the bushfire, a result confirmed by eddy covariance data, remote sensing, and modeling. Such a fast evapotranspiration recovery is likely generalizable to tall eucalypt forests in south-eastern Australia as shown by remote sensing. Once climate variability is discounted, ecohydrological modeling shows evapotranspiration rates from the recovering forest which reach peak values of +20% evapotranspiration 3 years post-fire. As a result, modeled runoff decreases substantially. Contrary to previous research, we find that the increase in modeled evapotranspiration is largely caused by the aerodynamic effects of a much shorter forest height leading to higher surface temperature, higher humidity gradients and therefore increased transpiration. However, increases in evapotranspiration as well as decreases in runoff caused by the young forest are constrained by energy and water limitations. Our result of an increase in evapotranspiration due to aerodynamic warming in a shorter forest after wildfires could occur in many parts of the world experiencing forest disturbances."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"oa":1,"author":[{"full_name":"Meili, Naika","first_name":"Naika","last_name":"Meili"},{"last_name":"Beringer","first_name":"Jason","full_name":"Beringer, Jason"},{"first_name":"Jiacheng","full_name":"Zhao, Jiacheng","last_name":"Zhao"},{"full_name":"Fatichi, Simone","first_name":"Simone","id":"cf8e546b-a9b0-11f0-a43b-aa89ed1b56d6","last_name":"Fatichi"}],"intvolume":"        30","pmid":1,"scopus_import":"1","quality_controlled":"1","OA_type":"hybrid","status":"public","volume":30,"year":"2024","publication":"Global Change Biology","das_tickbox":"1","date_updated":"2026-08-11T05:51:07Z","date_created":"2026-07-27T12:30:24Z","date_published":"2024-01-01T00:00:00Z","OA_place":"publisher","month":"01"},{"date_updated":"2026-08-12T08:44:53Z","das_tickbox":"1","date_created":"2026-07-27T12:30:24Z","date_published":"2024-01-01T00:00:00Z","OA_place":"publisher","month":"01","publisher":"Wiley","abstract":[{"lang":"eng","text":"The ascent of water from the soil to the leaves of vascular plants, described by the study of plant hydraulics, regulates ecosystem responses to environmental forcing and recovery from stress periods. Several approaches to model plant hydraulics have been proposed. In this study, we introduce four different versions of plant hydraulics representations in the terrestrial biosphere model T&C to understand the significance of plant hydraulics to ecosystem functioning. We tested representations of plant hydraulics, investigating plant water capacitance, and long-term xylem damages following drought. The four models we tested were a combination of representations including or neglecting capacitance and including or neglecting xylem damage legacies. Using the models at six case studies spanning semiarid to tropical ecosystems, we quantify how plant xylem flow, plant water storage and long-term xylem damage can modulate overall water and carbon dynamics across multiple time scales. We show that as drought develops, models with plant hydraulics predict a slower onset of plant water stress, and a diurnal variability of water and carbon fluxes closer to observations. Plant water storage was found to be particularly important for the diurnal dynamics of water and carbon fluxes, with models that include plant water capacitance yielding better results. Models including permanent damage to conducting plant tissues show an additional significant drought legacy effect, limiting plant productivity during the recovery phase following major droughts. However, when considering ecosystem responses to the observed climate variability, plant hydraulic modules alone cannot significantly improve the overall model performance, even though they reproduce more realistic water and carbon dynamics. This opens new avenues for model development, explicitly linking plant hydraulics with additional ecosystem processes, such as plant phenology and improved carbon allocation algorithms."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"author":[{"full_name":"Paschalis, Athanasios","first_name":"Athanasios","last_name":"Paschalis"},{"last_name":"De Kauwe","full_name":"De Kauwe, Martin G.","first_name":"Martin G."},{"last_name":"Sabot","first_name":"Manon","full_name":"Sabot, Manon"},{"last_name":"Fatichi","full_name":"Fatichi, Simone","id":"cf8e546b-a9b0-11f0-a43b-aa89ed1b56d6","first_name":"Simone"}],"oa":1,"intvolume":"        30","OA_type":"hybrid","quality_controlled":"1","status":"public","scopus_import":"1","pmid":1,"publication":"Global Change Biology","volume":30,"year":"2024","citation":{"chicago":"Paschalis, Athanasios, Martin G. De Kauwe, Manon Sabot, and Simone Fatichi. “When Do Plant Hydraulics Matter in Terrestrial Biosphere Modelling?” <i>Global Change Biology</i>. Wiley, 2024. <a href=\"https://doi.org/10.1111/gcb.17022\">https://doi.org/10.1111/gcb.17022</a>.","ista":"Paschalis A, De Kauwe MG, Sabot M, Fatichi S. 2024. When do plant hydraulics matter in terrestrial biosphere modelling? Global Change Biology. 30(1), e17022.","apa":"Paschalis, A., De Kauwe, M. G., Sabot, M., &#38; Fatichi, S. (2024). When do plant hydraulics matter in terrestrial biosphere modelling? <i>Global Change Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/gcb.17022\">https://doi.org/10.1111/gcb.17022</a>","short":"A. Paschalis, M.G. De Kauwe, M. Sabot, S. Fatichi, Global Change Biology 30 (2024).","ama":"Paschalis A, De Kauwe MG, Sabot M, Fatichi S. When do plant hydraulics matter in terrestrial biosphere modelling? <i>Global Change Biology</i>. 2024;30(1). doi:<a href=\"https://doi.org/10.1111/gcb.17022\">10.1111/gcb.17022</a>","mla":"Paschalis, Athanasios, et al. “When Do Plant Hydraulics Matter in Terrestrial Biosphere Modelling?” <i>Global Change Biology</i>, vol. 30, no. 1, e17022, Wiley, 2024, doi:<a href=\"https://doi.org/10.1111/gcb.17022\">10.1111/gcb.17022</a>.","ieee":"A. Paschalis, M. G. De Kauwe, M. Sabot, and S. Fatichi, “When do plant hydraulics matter in terrestrial biosphere modelling?,” <i>Global Change Biology</i>, vol. 30, no. 1. Wiley, 2024."},"main_file_link":[{"url":" https://doi.org/10.1111/gcb.17022","open_access":"1"}],"extern":"1","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_number":"e17022","publication_identifier":{"eissn":["1365-2486"],"issn":["1354-1013"]},"publication_status":"published","external_id":{"pmid":["37962234 "]},"title":"When do plant hydraulics matter in terrestrial biosphere modelling?","issue":"1","article_type":"original","type":"journal_article","article_processing_charge":"No","doi":"10.1111/gcb.17022","fulldoi":"https://doi.org/10.1111/gcb.17022","language":[{"iso":"eng"}],"oa_version":"Published Version","keyword":["Ecosystem recovery","Ecosystem responses","Plant hydraulics","Terrestrial biosphere model","Water stress"],"_id":"22563","day":"01"},{"date_published":"2024-01-01T00:00:00Z","month":"01","OA_place":"publisher","das_tickbox":"1","date_updated":"2026-08-12T08:53:18Z","date_created":"2026-07-27T12:30:25Z","scopus_import":"1","OA_type":"hybrid","status":"public","quality_controlled":"1","intvolume":"       150","volume":150,"year":"2024","publication":"Quarterly Journal of the Royal Meteorological Society","publisher":"Wiley","oa":1,"author":[{"last_name":"Lipson","first_name":"Mathew J.","full_name":"Lipson, Mathew J."},{"last_name":"Grimmond","full_name":"Grimmond, Sue","first_name":"Sue"},{"first_name":"Martin","full_name":"Best, Martin","last_name":"Best"},{"first_name":"Gab","full_name":"Abramowitz, Gab","last_name":"Abramowitz"},{"first_name":"Andrew","full_name":"Coutts, Andrew","last_name":"Coutts"},{"full_name":"Tapper, Nigel","first_name":"Nigel","last_name":"Tapper"},{"full_name":"Baik, Jong‐Jin","first_name":"Jong‐Jin","last_name":"Baik"},{"first_name":"Meiring","full_name":"Beyers, Meiring","last_name":"Beyers"},{"first_name":"Lewis","full_name":"Blunn, Lewis","last_name":"Blunn"},{"first_name":"Souhail","full_name":"Boussetta, Souhail","last_name":"Boussetta"},{"last_name":"Bou‐Zeid","first_name":"Elie","full_name":"Bou‐Zeid, Elie"},{"full_name":"De Kauwe, Martin G.","first_name":"Martin G.","last_name":"De Kauwe"},{"last_name":"de Munck","full_name":"de Munck, Cécile","first_name":"Cécile"},{"last_name":"Demuzere","first_name":"Matthias","full_name":"Demuzere, Matthias"},{"last_name":"Fatichi","full_name":"Fatichi, Simone","first_name":"Simone","id":"cf8e546b-a9b0-11f0-a43b-aa89ed1b56d6"},{"full_name":"Fortuniak, Krzysztof","first_name":"Krzysztof","last_name":"Fortuniak"},{"last_name":"Han","first_name":"Beom‐Soon","full_name":"Han, Beom‐Soon"},{"last_name":"Hendry","first_name":"Margaret A.","full_name":"Hendry, Margaret A."},{"full_name":"Kikegawa, Yukihiro","first_name":"Yukihiro","last_name":"Kikegawa"},{"last_name":"Kondo","full_name":"Kondo, Hiroaki","first_name":"Hiroaki"},{"full_name":"Lee, Doo‐Il","first_name":"Doo‐Il","last_name":"Lee"},{"last_name":"Lee","full_name":"Lee, Sang‐Hyun","first_name":"Sang‐Hyun"},{"last_name":"Lemonsu","full_name":"Lemonsu, Aude","first_name":"Aude"},{"first_name":"Tiago","full_name":"Machado, Tiago","last_name":"Machado"},{"full_name":"Manoli, Gabriele","first_name":"Gabriele","last_name":"Manoli"},{"first_name":"Alberto","full_name":"Martilli, Alberto","last_name":"Martilli"},{"full_name":"Masson, Valéry","first_name":"Valéry","last_name":"Masson"},{"first_name":"Joe","full_name":"McNorton, Joe","last_name":"McNorton"},{"last_name":"Meili","full_name":"Meili, Naika","first_name":"Naika"},{"last_name":"Meyer","first_name":"David","full_name":"Meyer, David"},{"first_name":"Kerry A.","full_name":"Nice, Kerry A.","last_name":"Nice"},{"last_name":"Oleson","full_name":"Oleson, Keith W.","first_name":"Keith W."},{"last_name":"Park","first_name":"Seung‐Bu","full_name":"Park, Seung‐Bu"},{"last_name":"Roth","first_name":"Michael","full_name":"Roth, Michael"},{"last_name":"Schoetter","full_name":"Schoetter, Robert","first_name":"Robert"},{"full_name":"Simón‐Moral, Andrés","first_name":"Andrés","last_name":"Simón‐Moral"},{"first_name":"Gert‐Jan","full_name":"Steeneveld, Gert‐Jan","last_name":"Steeneveld"},{"full_name":"Sun, Ting","first_name":"Ting","last_name":"Sun"},{"last_name":"Takane","first_name":"Yuya","full_name":"Takane, Yuya"},{"full_name":"Thatcher, Marcus","first_name":"Marcus","last_name":"Thatcher"},{"first_name":"Aristofanis","full_name":"Tsiringakis, Aristofanis","last_name":"Tsiringakis"},{"full_name":"Varentsov, Mikhail","first_name":"Mikhail","last_name":"Varentsov"},{"full_name":"Wang, Chenghao","first_name":"Chenghao","last_name":"Wang"},{"full_name":"Wang, Zhi‐Hua","first_name":"Zhi‐Hua","last_name":"Wang"},{"last_name":"Pitman","full_name":"Pitman, Andy J.","first_name":"Andy J."}],"abstract":[{"text":"Accurately predicting weather and climate in cities is critical for safeguarding human health and strengthening urban resilience. Multimodel evaluations can lead to model improvements; however, there have been no major intercomparisons of urban-focussed land surface models in over a decade. Here, in Phase 1 of the Urban-PLUMBER project, we evaluate the ability of 30 land surface models to simulate surface energy fluxes critical to atmospheric meteorological and air quality simulations. We establish minimum and upper performance expectations for participating models using simple information-limited models as benchmarks. Compared with the last major model intercomparison at the same site, we find broad improvement in the current cohort's predictions of short-wave radiation, sensible and latent heat fluxes, but little or no improvement in long-wave radiation and momentum fluxes. Models with a simple urban representation (e.g., ‘slab’ schemes) generally perform well, particularly when combined with sophisticated hydrological/vegetation models. Some mid-complexity models (e.g., ‘canyon’ schemes) also perform well, indicating efforts to integrate vegetation and hydrology processes have paid dividends. The most complex models that resolve three-dimensional interactions between buildings in general did not perform as well as other categories. However, these models also tended to have the simplest representations of hydrology and vegetation. Models without any urban representation (i.e., vegetation-only land surface models) performed poorly for latent heat fluxes, and reasonably for other energy fluxes at this suburban site. Our analysis identified widespread human errors in initial submissions that substantially affected model performances. Although significant efforts are applied to correct these errors, we conclude that human factors are likely to influence results in this (or any) model intercomparison, particularly where participating scientists have varying experience and first languages. These initial results are for one suburban site, and future phases of Urban-PLUMBER will evaluate models across 20 sites in different urban and regional climate zones.","lang":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","title":"Evaluation of 30 urban land surface models in the Urban-PLUMBER project: Phase 1 results","publication_status":"published","publication_identifier":{"eissn":["1477-870X"],"issn":["0035-9009"]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1002/qj.4589"}],"extern":"1","citation":{"ieee":"M. J. Lipson <i>et al.</i>, “Evaluation of 30 urban land surface models in the Urban-PLUMBER project: Phase 1 results,” <i>Quarterly Journal of the Royal Meteorological Society</i>, vol. 150, no. 758. Wiley, pp. 126–169, 2024.","mla":"Lipson, Mathew J., et al. “Evaluation of 30 Urban Land Surface Models in the Urban-PLUMBER Project: Phase 1 Results.” <i>Quarterly Journal of the Royal Meteorological Society</i>, vol. 150, no. 758, Wiley, 2024, pp. 126–69, doi:<a href=\"https://doi.org/10.1002/qj.4589\">10.1002/qj.4589</a>.","short":"M.J. Lipson, S. Grimmond, M. Best, G. Abramowitz, A. Coutts, N. Tapper, J. Baik, M. Beyers, L. Blunn, S. Boussetta, E. Bou‐Zeid, M.G. De Kauwe, C. de Munck, M. Demuzere, S. Fatichi, K. Fortuniak, B. Han, M.A. Hendry, Y. Kikegawa, H. Kondo, D. Lee, S. Lee, A. Lemonsu, T. Machado, G. Manoli, A. Martilli, V. Masson, J. McNorton, N. Meili, D. Meyer, K.A. Nice, K.W. Oleson, S. Park, M. Roth, R. Schoetter, A. Simón‐Moral, G. Steeneveld, T. Sun, Y. Takane, M. Thatcher, A. Tsiringakis, M. Varentsov, C. Wang, Z. Wang, A.J. Pitman, Quarterly Journal of the Royal Meteorological Society 150 (2024) 126–169.","ama":"Lipson MJ, Grimmond S, Best M, et al. Evaluation of 30 urban land surface models in the Urban-PLUMBER project: Phase 1 results. <i>Quarterly Journal of the Royal Meteorological Society</i>. 2024;150(758):126-169. doi:<a href=\"https://doi.org/10.1002/qj.4589\">10.1002/qj.4589</a>","apa":"Lipson, M. J., Grimmond, S., Best, M., Abramowitz, G., Coutts, A., Tapper, N., … Pitman, A. J. (2024). Evaluation of 30 urban land surface models in the Urban-PLUMBER project: Phase 1 results. <i>Quarterly Journal of the Royal Meteorological Society</i>. Wiley. <a href=\"https://doi.org/10.1002/qj.4589\">https://doi.org/10.1002/qj.4589</a>","ista":"Lipson MJ, Grimmond S, Best M, Abramowitz G, Coutts A, Tapper N, Baik J, Beyers M, Blunn L, Boussetta S, Bou‐Zeid E, De Kauwe MG, de Munck C, Demuzere M, Fatichi S, Fortuniak K, Han B, Hendry MA, Kikegawa Y, Kondo H, Lee D, Lee S, Lemonsu A, Machado T, Manoli G, Martilli A, Masson V, McNorton J, Meili N, Meyer D, Nice KA, Oleson KW, Park S, Roth M, Schoetter R, Simón‐Moral A, Steeneveld G, Sun T, Takane Y, Thatcher M, Tsiringakis A, Varentsov M, Wang C, Wang Z, Pitman AJ. 2024. Evaluation of 30 urban land surface models in the Urban-PLUMBER project: Phase 1 results. Quarterly Journal of the Royal Meteorological Society. 150(758), 126–169.","chicago":"Lipson, Mathew J., Sue Grimmond, Martin Best, Gab Abramowitz, Andrew Coutts, Nigel Tapper, Jong‐Jin Baik, et al. “Evaluation of 30 Urban Land Surface Models in the Urban-PLUMBER Project: Phase 1 Results.” <i>Quarterly Journal of the Royal Meteorological Society</i>. Wiley, 2024. <a href=\"https://doi.org/10.1002/qj.4589\">https://doi.org/10.1002/qj.4589</a>."},"article_processing_charge":"No","day":"01","_id":"22583","keyword":["Benchmark","Energy balance","Intercomparison","Model evaluation","Urban climate","Urban meteorology"],"oa_version":"Published Version","fulldoi":"https://doi.org/10.1002/qj.4589","language":[{"iso":"eng"}],"doi":"10.1002/qj.4589","article_type":"original","page":"126-169","issue":"758","type":"journal_article"},{"date_published":"2024-07-22T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_place":"repository","month":"07","title":"Data and code for: Integral points on cubic surfaces: heuristics and numerics","date_updated":"2026-08-12T12:15:33Z","citation":{"ista":"Browning TD, Sutherland A. 2024. Data and code for: Integral points on cubic surfaces: heuristics and numerics, Göttingen Research Online Data, <a href=\"https://doi.org/10.25625/4FLFH8\">10.25625/4FLFH8</a>.","chicago":"Browning, Timothy D, and Andrew Sutherland. “Data and Code for: Integral Points on Cubic Surfaces: Heuristics and Numerics.” Göttingen Research Online Data, 2024. <a href=\"https://doi.org/10.25625/4FLFH8\">https://doi.org/10.25625/4FLFH8</a>.","apa":"Browning, T. D., &#38; Sutherland, A. (2024). Data and code for: Integral points on cubic surfaces: heuristics and numerics. Göttingen Research Online Data. <a href=\"https://doi.org/10.25625/4FLFH8\">https://doi.org/10.25625/4FLFH8</a>","ieee":"T. D. Browning and A. Sutherland, “Data and code for: Integral points on cubic surfaces: heuristics and numerics.” Göttingen Research Online Data, 2024.","mla":"Browning, Timothy D., and Andrew Sutherland. <i>Data and Code for: Integral Points on Cubic Surfaces: Heuristics and Numerics</i>. Göttingen Research Online Data, 2024, doi:<a href=\"https://doi.org/10.25625/4FLFH8\">10.25625/4FLFH8</a>.","short":"T.D. Browning, A. Sutherland, (2024).","ama":"Browning TD, Sutherland A. Data and code for: Integral points on cubic surfaces: heuristics and numerics. 2024. doi:<a href=\"https://doi.org/10.25625/4FLFH8\">10.25625/4FLFH8</a>"},"main_file_link":[{"url":"https://doi.org/10.25625/4FLFH8","open_access":"1"}],"date_created":"2026-07-02T10:38:53Z","department":[{"_id":"TiBr"}],"article_processing_charge":"No","OA_type":"green","status":"public","ddc":["500"],"doi":"10.25625/4FLFH8","fulldoi":"https://doi.org/10.25625/4FLFH8","oa_version":"Published Version","year":"2024","_id":"22234","contributor":[{"last_name":"Wilsch","first_name":"Florian Alexander","id":"560601DA-8D36-11E9-A136-7AC1E5697425","orcid":"0000-0001-7302-8256"}],"day":"22","publisher":"Göttingen Research Online Data","related_material":{"record":[{"relation":"used_in_publication","id":"20249","status":"public"}]},"corr_author":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"text":"Lists of nontrivial integral points on some (families of) cubic surfaces. (2024-07-18)","lang":"eng"}],"author":[{"last_name":"Browning","id":"35827D50-F248-11E8-B48F-1D18A9856A87","first_name":"Timothy D","orcid":"0000-0002-8314-0177","full_name":"Browning, Timothy D"},{"last_name":"Sutherland","first_name":"Andrew","full_name":"Sutherland, Andrew"}],"has_accepted_license":"1","type":"research_data_reference","oa":1},{"arxiv":1,"related_material":{"link":[{"url":" https://github.com/egozverev/Shold-It-Be-Executed-Or-Processed","relation":"software"}]},"type":"preprint","has_accepted_license":"1","article_processing_charge":"No","day":"01","_id":"19063","fulldoi":"https://doi.org/10.48550/arXiv.2403.06833","oa_version":"Preprint","language":[{"iso":"eng"}],"doi":"10.48550/arXiv.2403.06833","citation":{"apa":"Zverev, E., Abdelnabi, S., Tabesh, S., Fritz, M., &#38; Lampert, C. (n.d.). Can LLMs separate instructions from data? And what do we even mean by that? <i>arXiv</i>. <a href=\"https://doi.org/10.48550/arXiv.2403.06833\">https://doi.org/10.48550/arXiv.2403.06833</a>","ieee":"E. Zverev, S. Abdelnabi, S. Tabesh, M. Fritz, and C. Lampert, “Can LLMs separate instructions from data? And what do we even mean by that?,” <i>arXiv</i>. .","mla":"Zverev, Egor, et al. “Can LLMs Separate Instructions from Data? And What Do We Even Mean by That?” <i>ArXiv</i>, 2403.06833, doi:<a href=\"https://doi.org/10.48550/arXiv.2403.06833\">10.48550/arXiv.2403.06833</a>.","ama":"Zverev E, Abdelnabi S, Tabesh S, Fritz M, Lampert C. Can LLMs separate instructions from data? And what do we even mean by that? <i>arXiv</i>. doi:<a href=\"https://doi.org/10.48550/arXiv.2403.06833\">10.48550/arXiv.2403.06833</a>","short":"E. Zverev, S. Abdelnabi, S. Tabesh, M. Fritz, C. Lampert, ArXiv (n.d.).","ista":"Zverev E, Abdelnabi S, Tabesh S, Fritz M, Lampert C. Can LLMs separate instructions from data? And what do we even mean by that? arXiv, 2403.06833.","chicago":"Zverev, Egor, Sahar Abdelnabi, Soroush Tabesh, Mario Fritz, and Christoph Lampert. “Can LLMs Separate Instructions from Data? And What Do We Even Mean by That?” <i>ArXiv</i>, n.d. <a href=\"https://doi.org/10.48550/arXiv.2403.06833\">https://doi.org/10.48550/arXiv.2403.06833</a>."},"acknowledged_ssus":[{"_id":"ScienComp"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_number":"2403.06833","title":"Can LLMs separate instructions from data? And what do we even mean by that?","external_id":{"arxiv":["2403.06833"]},"publication_status":"submitted","oa":1,"file_date_updated":"2025-02-20T10:11:45Z","author":[{"full_name":"Zverev, Egor","first_name":"Egor","id":"05162b19-1340-11ed-8f02-fa94e0e8c3bc","last_name":"Zverev"},{"full_name":"Abdelnabi, Sahar","first_name":"Sahar","last_name":"Abdelnabi"},{"full_name":"Tabesh, Soroush","orcid":"0009-0003-4119-6281","id":"06000900-6068-11ef-8d61-c2472ef2e752","first_name":"Soroush","last_name":"Tabesh"},{"last_name":"Fritz","first_name":"Mario","full_name":"Fritz, Mario"},{"full_name":"Lampert, Christoph","id":"40C20FD2-F248-11E8-B48F-1D18A9856A87","first_name":"Christoph","orcid":"0000-0001-8622-7887","last_name":"Lampert"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"corr_author":"1","abstract":[{"text":"Instruction-tuned Large Language Models (LLMs) show impressive results in numerous practical applications, but they lack essential safety features that are common in other areas of computer science, particularly an explicit separation of instructions and data. This makes them vulnerable to manipulations such as indirect prompt injections and generally unsuitable for safety-critical tasks. Surprisingly, there is currently no established definition or benchmark to quantify this phenomenon. In this work, we close this gap by introducing a formal measure for instruction-data separation and an empirical variant that is calculable from a model's outputs. We also present a new dataset, SEP, that allows estimating the measure for real-world models. Our results on various LLMs show that the problem of instruction-data separation is real: all models fail to achieve high separation, and canonical mitigation techniques, such as prompt engineering and fine-tuning, either fail to substantially improve separation or reduce model utility. The source code and SEP dataset are openly accessible at https://github.com/egozverev/Shold-It-Be-Executed-Or-Processed.\r\n","lang":"eng"}],"OA_type":"green","ddc":["000"],"status":"public","year":"2024","publication":"arXiv","file":[{"access_level":"open_access","date_updated":"2025-02-20T10:11:45Z","creator":"ezverev","checksum":"35eb43968684b87be59144603ef10af0","success":1,"date_created":"2025-02-20T10:11:45Z","file_name":"2403.06833v3.pdf","content_type":"application/pdf","relation":"main_file","file_size":530972,"file_id":"19064"}],"date_updated":"2026-08-13T07:26:54Z","department":[{"_id":"GradSch"},{"_id":"ChLa"}],"acknowledgement":"The authors would like to sincerely thank Juan Rocamonde for valuable feedback to our manuscript. We acknowledge the support from the Scientific Service Units (SSU) of ISTA through resources provided by Scientific Computing (SciComp). We thank Dan Alistarh for providing us with computational resources. This work was partially funded by the German Federal Ministry of Education and Research (BMBF) under the grant AIgenCY (16KIS2012) and ELSA – European Lighthouse on Secure and Safe AI funded by the European Union under grant agreement No. 101070617. Views and opinions expressed are however those of the authors only and do not necessarily reflect those of the European Union or European Commission. Neither the European Union nor the European Commission can be held responsible for them.","date_created":"2025-02-20T10:13:42Z","date_published":"2024-03-01T00:00:00Z","month":"03","OA_place":"repository"},{"citation":{"chicago":"Aebischer, Kathrin, Lea Marie Becker, Paul Schanda, and Matthias Ernst. “Evaluating the Motional Timescales Contributing to Averaged Anisotropic Interactions in MAS Solid-State NMR.” <i>Magnetic Resonance</i>. Copernicus Publications, 2024. <a href=\"https://doi.org/10.5194/mr-5-69-2024\">https://doi.org/10.5194/mr-5-69-2024</a>.","ista":"Aebischer K, Becker LM, Schanda P, Ernst M. 2024. Evaluating the motional timescales contributing to averaged anisotropic interactions in MAS solid-state NMR. Magnetic Resonance. 5(1), 69–86.","apa":"Aebischer, K., Becker, L. M., Schanda, P., &#38; Ernst, M. (2024). Evaluating the motional timescales contributing to averaged anisotropic interactions in MAS solid-state NMR. <i>Magnetic Resonance</i>. Copernicus Publications. <a href=\"https://doi.org/10.5194/mr-5-69-2024\">https://doi.org/10.5194/mr-5-69-2024</a>","ama":"Aebischer K, Becker LM, Schanda P, Ernst M. Evaluating the motional timescales contributing to averaged anisotropic interactions in MAS solid-state NMR. <i>Magnetic Resonance</i>. 2024;5(1):69-86. doi:<a href=\"https://doi.org/10.5194/mr-5-69-2024\">10.5194/mr-5-69-2024</a>","short":"K. Aebischer, L.M. Becker, P. Schanda, M. Ernst, Magnetic Resonance 5 (2024) 69–86.","ieee":"K. Aebischer, L. M. Becker, P. Schanda, and M. Ernst, “Evaluating the motional timescales contributing to averaged anisotropic interactions in MAS solid-state NMR,” <i>Magnetic Resonance</i>, vol. 5, no. 1. Copernicus Publications, pp. 69–86, 2024.","mla":"Aebischer, Kathrin, et al. “Evaluating the Motional Timescales Contributing to Averaged Anisotropic Interactions in MAS Solid-State NMR.” <i>Magnetic Resonance</i>, vol. 5, no. 1, Copernicus Publications, 2024, pp. 69–86, doi:<a href=\"https://doi.org/10.5194/mr-5-69-2024\">10.5194/mr-5-69-2024</a>."},"title":"Evaluating the motional timescales contributing to averaged anisotropic interactions in MAS solid-state NMR","external_id":{"pmid":["40384772"]},"publication_identifier":{"eissn":["2699-0016"]},"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","has_accepted_license":"1","project":[{"_id":"7be609c4-9f16-11ee-852c-85015ce2b9b0","name":"Exploring protein dynamics by solid-state MAS NMR through specific labeling approaches","grant_number":"26777"}],"article_type":"original","page":"69-86","issue":"1","related_material":{"record":[{"id":"22906","relation":"research_data","status":"public"}]},"day":"11","_id":"17161","language":[{"iso":"eng"}],"fulldoi":"https://doi.org/10.5194/mr-5-69-2024","oa_version":"Published Version","doi":"10.5194/mr-5-69-2024","article_processing_charge":"Yes","department":[{"_id":"PaSc"}],"date_created":"2024-06-23T22:01:02Z","acknowledgement":"We would like to thank Kay Saalwächter for pointing out important aspects of the intermediate regime during the open review process. Lea Marie Becker is recipient of a DOC fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology Austria.\r\nThis research has been supported by the Österreichischen Akademie der Wissenschaften (grant no. PR10660EAW01) and the Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (grant nos. 200020_188988 and 200020_219375).","file":[{"file_size":6736194,"file_id":"17181","content_type":"application/pdf","relation":"main_file","checksum":"d01074f6919387fcaf8c9ebed320ccae","success":1,"date_created":"2024-06-27T06:42:55Z","file_name":"2024_MagneticResonance_Aebischer.pdf","creator":"dernst","access_level":"open_access","date_updated":"2024-06-27T06:42:55Z"}],"date_updated":"2026-09-11T08:49:55Z","month":"06","date_published":"2024-06-11T00:00:00Z","file_date_updated":"2024-06-27T06:42:55Z","oa":1,"author":[{"last_name":"Aebischer","first_name":"Kathrin","full_name":"Aebischer, Kathrin"},{"last_name":"Becker","id":"36336939-eb97-11eb-a6c2-c83f1214ca79","first_name":"Lea Marie","orcid":"0000-0002-6401-5151","full_name":"Becker, Lea Marie"},{"full_name":"Schanda, Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","first_name":"Paul","orcid":"0000-0002-9350-7606","last_name":"Schanda"},{"last_name":"Ernst","first_name":"Matthias","full_name":"Ernst, Matthias"}],"abstract":[{"text":"Dynamic processes in molecules can occur on a wide range of timescales, and it is important to understand which timescales of motion contribute to different parameters used in dynamics measurements. For spin relaxation, this can easily be understood from the sampling frequencies of the spectral-density function by different relaxation-rate constants. In addition to data from relaxation measurements, determining dynamically averaged anisotropic interactions in magic-angle spinning (MAS) solid-state NMR allows for better quantification of the amplitude of molecular motion. For partially averaged anisotropic interactions, the relevant timescales of motion are not so clearly defined. Whether the averaging depends on the experimental methods (e.g., pulse sequences) or conditions (e.g., MAS frequency, magnitude of anisotropic interaction, radio-frequency field amplitudes) is not fully understood. To investigate these questions, we performed numerical simulations of dynamic systems based on the stochastic Liouville equation using several experiments for recoupling the dipolar coupling, chemical-shift anisotropy or quadrupolar coupling. As described in the literature, the transition between slow motion, where parameters characterizing the anisotropic interaction are not averaged, and fast motion, where the tensors are averaged leading to a scaled anisotropic quantity, occurs over a window of motional rate constants that depends mainly on the strength of the interaction. This transition region can span 2 orders of magnitude in exchange-rate constants (typically in the microsecond range) but depends only marginally on the employed recoupling scheme or sample spinning frequency. The transition region often coincides with a fast relaxation of coherences, making precise quantitative measurements difficult. Residual couplings in off-magic-angle experiments, however, average over longer timescales of motion. While in principle one may gain information on the timescales of motion from the transition area, extracting such information is hampered by low signal-to-noise ratio in experimental spectra due to fast relaxation that occurs in the same region.","lang":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"publisher":"Copernicus Publications","year":"2024","volume":5,"publication":"Magnetic Resonance","scopus_import":"1","pmid":1,"status":"public","ddc":["530"],"quality_controlled":"1","intvolume":"         5"},{"doi":"10.3929/ethz-b-000666765","fulldoi":"https://doi.org/10.3929/ethz-b-000666765","oa_version":"None","_id":"22906","contributor":[{"last_name":"Ernst","contributor_type":"contact_person","first_name":"Matthias"},{"last_name":"Aebischer","contributor_type":"data_collector","first_name":"Kathrin"},{"contributor_type":"data_collector","last_name":"Ernst","first_name":"Matthias"},{"contributor_type":"data_collector","last_name":"Schanda","first_name":"Paul"},{"last_name":"Becker","contributor_type":"data_collector","first_name":"Lea Marie"},{"contributor_type":"producer","last_name":" Ernst","first_name":"Matthias"},{"contributor_type":"producer","last_name":" Aebischer","first_name":"Kathrin"},{"contributor_type":"producer","last_name":"Schanda","first_name":"Paul "},{"first_name":"Lea Marie","contributor_type":"producer","last_name":"Becker"}],"year":"2024","day":"03","article_processing_charge":"No","status":"public","ddc":["530"],"OA_type":"green","author":[{"full_name":"Aebischer, Kathrin","first_name":"Kathrin","last_name":"Aebischer"}],"oa":1,"type":"research_data_reference","publisher":"Repository","related_material":{"record":[{"status":"public","id":"17161","relation":"used_in_publication"}]},"OA_place":"repository","month":"04","title":"Evaluating the motional time scales contributing to averaged anisotropic interactions in MAS solid-state NMR","date_published":"2024-04-03T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2026-09-11T08:49:48Z","department":[{"_id":"PaSc"}],"date_updated":"2026-09-11T08:49:55Z","citation":{"chicago":"Aebischer, Kathrin. “Evaluating the Motional Time Scales Contributing to Averaged Anisotropic Interactions in MAS Solid-State NMR.” Repository, 2024. <a href=\"https://doi.org/10.3929/ethz-b-000666765\">https://doi.org/10.3929/ethz-b-000666765</a>.","ista":"Aebischer K. 2024. Evaluating the motional time scales contributing to averaged anisotropic interactions in MAS solid-state NMR, Repository, <a href=\"https://doi.org/10.3929/ethz-b-000666765\">10.3929/ethz-b-000666765</a>.","apa":"Aebischer, K. (2024). Evaluating the motional time scales contributing to averaged anisotropic interactions in MAS solid-state NMR. Repository. <a href=\"https://doi.org/10.3929/ethz-b-000666765\">https://doi.org/10.3929/ethz-b-000666765</a>","short":"K. Aebischer, (2024).","ama":"Aebischer K. Evaluating the motional time scales contributing to averaged anisotropic interactions in MAS solid-state NMR. 2024. doi:<a href=\"https://doi.org/10.3929/ethz-b-000666765\">10.3929/ethz-b-000666765</a>","ieee":"K. Aebischer, “Evaluating the motional time scales contributing to averaged anisotropic interactions in MAS solid-state NMR.” Repository, 2024.","mla":"Aebischer, Kathrin. <i>Evaluating the Motional Time Scales Contributing to Averaged Anisotropic Interactions in MAS Solid-State NMR</i>. Repository, 2024, doi:<a href=\"https://doi.org/10.3929/ethz-b-000666765\">10.3929/ethz-b-000666765</a>."},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.3929/ethz-b-000666765"}]},{"title":"Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons","publication_identifier":{"issn":["1544-9173"],"eissn":["1545-7885"]},"publication_status":"published","external_id":{"pmid":["39556620"],"isi":["001358568700003"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"e3002879","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"PreCl"}],"citation":{"ista":"Kim O, Okamoto Y, Kaufmann W, Brose N, Shigemoto R, Jonas PM. 2024. Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons. PLoS Biology. 22(11), e3002879.","chicago":"Kim, Olena, Yuji Okamoto, Walter Kaufmann, Nils Brose, Ryuichi Shigemoto, and Peter M Jonas. “Presynaptic CAMP-PKA-Mediated Potentiation Induces Reconfiguration of Synaptic Vesicle Pools and Channel-Vesicle Coupling at Hippocampal Mossy Fiber Boutons.” <i>PLoS Biology</i>. Public Library of Science, 2024. <a href=\"https://doi.org/10.1371/journal.pbio.3002879\">https://doi.org/10.1371/journal.pbio.3002879</a>.","ieee":"O. Kim, Y. Okamoto, W. Kaufmann, N. Brose, R. Shigemoto, and P. M. Jonas, “Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons,” <i>PLoS Biology</i>, vol. 22, no. 11. Public Library of Science, 2024.","mla":"Kim, Olena, et al. “Presynaptic CAMP-PKA-Mediated Potentiation Induces Reconfiguration of Synaptic Vesicle Pools and Channel-Vesicle Coupling at Hippocampal Mossy Fiber Boutons.” <i>PLoS Biology</i>, vol. 22, no. 11, e3002879, Public Library of Science, 2024, doi:<a href=\"https://doi.org/10.1371/journal.pbio.3002879\">10.1371/journal.pbio.3002879</a>.","ama":"Kim O, Okamoto Y, Kaufmann W, Brose N, Shigemoto R, Jonas PM. Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons. <i>PLoS Biology</i>. 2024;22(11). doi:<a href=\"https://doi.org/10.1371/journal.pbio.3002879\">10.1371/journal.pbio.3002879</a>","short":"O. Kim, Y. Okamoto, W. Kaufmann, N. Brose, R. Shigemoto, P.M. Jonas, PLoS Biology 22 (2024).","apa":"Kim, O., Okamoto, Y., Kaufmann, W., Brose, N., Shigemoto, R., &#38; Jonas, P. M. (2024). Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons. <i>PLoS Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pbio.3002879\">https://doi.org/10.1371/journal.pbio.3002879</a>"},"fulldoi":"https://doi.org/10.1371/journal.pbio.3002879","oa_version":"Published Version","language":[{"iso":"eng"}],"_id":"18603","day":"18","doi":"10.1371/journal.pbio.3002879","article_processing_charge":"Yes","has_accepted_license":"1","project":[{"grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"},{"grant_number":"Z00312","name":"Synaptic communication in neuronal microcircuits","call_identifier":"FWF","_id":"25C5A090-B435-11E9-9278-68D0E5697425"},{"_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","name":"Mechanisms of GABA release in hippocampal circuits","grant_number":"P36232"},{"name":"Structural & functional basis of presynaptic plasticity","grant_number":"I06166","_id":"b1b85715-d554-11ed-a5ad-84a07fc9f18e"},{"_id":"25C3DBB6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"W01205","name":"Zellkommunikation in Gesundheit und Krankheit"},{"name":"FWF Open Access Fund","_id":"3AC91DDA-15DF-11EA-824D-93A3E7B544D1","call_identifier":"FWF"}],"type":"journal_article","article_type":"original","issue":"11","related_material":{"record":[{"status":"public","relation":"research_data","id":"18296"}]},"isi":1,"month":"11","OA_place":"publisher","date_published":"2024-11-18T00:00:00Z","department":[{"_id":"PeJo"},{"_id":"EM-Fac"},{"_id":"RySh"}],"acknowledgement":"We thank Carolina Borges-Merjane, Jing-Jing Chen, Katharina Lichter, and Samuel Young for critically reading the manuscript; the Electron Microscopy Facility of ISTA, in particular Vanessa Zheden, for extensive support, advice, and experimental assistance; the Preclinical Facility of ISTA, in particular Victoria Wimmer and Michael Schunn, for experimental assistance; Florian Marr and Christina Altmutter for technical support; Alois Schlögl for help with analysis; and Eleftheria Kralli-Beller for manuscript editing. We also thank Cordelia Imig for providing Munc13-1cKO-Munc13-2/3(−/−) mutant mice. Part of the work has been published in O.K.’s thesis in partial fulfillment of the requirements for the degree of Doctor of Philosophy.\r\nThis project received funding from the European Research Council and European Union’s Horizon 2020 research and innovation programme (ERC 692692 to P.J.; https://cordis.europa.eu/project/id/692692/de) and from the Fond zur Förderung der Wissenschaftlichen Forschung (Z312-B27 Wittgenstein award to P.J., https://www.fwf.ac.at/en/funding/portfolio/projects/fwf-wittgenstein-award; W1205-B09 and P36232-B to P.J., https://www.fwf.ac.at/en/funding; I6166-B to R.S.; https://www.fwf.ac.at/en/funding). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","date_created":"2024-12-01T23:01:54Z","ec_funded":1,"file":[{"file_id":"18608","file_size":3057631,"content_type":"application/pdf","relation":"main_file","file_name":"2024_PloSBio_Kim.pdf","success":1,"date_created":"2024-12-03T08:56:53Z","checksum":"7de2dcb50deb65dde05c80082bb85a82","creator":"dernst","date_updated":"2024-12-03T08:56:53Z","access_level":"open_access"}],"date_updated":"2026-04-16T12:20:34Z","publication":"PLoS Biology","volume":22,"year":"2024","APC_amount":"6248,82 EUR","OA_type":"gold","ddc":["570"],"status":"public","quality_controlled":"1","DOAJ_listed":"1","pmid":1,"scopus_import":"1","intvolume":"        22","author":[{"last_name":"Kim","full_name":"Kim, Olena","orcid":"0000-0003-2344-1039","first_name":"Olena","id":"3F8ABDDA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Okamoto","orcid":"0000-0003-0408-6094","first_name":"Yuji","id":"3337E116-F248-11E8-B48F-1D18A9856A87","full_name":"Okamoto, Yuji"},{"orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","full_name":"Kaufmann, Walter","last_name":"Kaufmann"},{"last_name":"Brose","full_name":"Brose, Nils","first_name":"Nils"},{"full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto"},{"full_name":"Jonas, Peter M","first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804","last_name":"Jonas"}],"oa":1,"file_date_updated":"2024-12-03T08:56:53Z","corr_author":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"text":"It is widely believed that information storage in neuronal circuits involves nanoscopic structural changes at synapses, resulting in the formation of synaptic engrams. However, direct evidence for this hypothesis is lacking. To test this conjecture, we combined chemical potentiation, functional analysis by paired pre-postsynaptic recordings, and structural analysis by electron microscopy (EM) and freeze-fracture replica labeling (FRL) at the rodent hippocampal mossy fiber synapse, a key synapse in the trisynaptic circuit of the hippocampus. Biophysical analysis of synaptic transmission revealed that forskolin-induced chemical potentiation increased the readily releasable vesicle pool size and vesicular release probability by 146% and 49%, respectively. Structural analysis of mossy fiber synapses by EM and FRL demonstrated an increase in the number of vesicles close to the plasma membrane and the number of clusters of the priming protein Munc13-1, indicating an increase in the number of both docked and primed vesicles. Furthermore, FRL analysis revealed a significant reduction of the distance between Munc13-1 and CaV2.1 Ca2+ channels, suggesting reconfiguration of the channel-vesicle coupling nanotopography. Our results indicate that presynaptic plasticity is associated with structural reorganization of active zones. We propose that changes in potential nanoscopic organization at synaptic vesicle release sites may be correlates of learning and memory at a plastic central synapse.","lang":"eng"}],"publisher":"Public Library of Science"},{"title":"Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons","user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","citation":{"ista":"Kim O. 2024. Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:18296\">10.15479/AT:ISTA:18296</a>.","chicago":"Kim, Olena. “Presynaptic CAMP-PKA-Mediated Potentiation Induces Reconfiguration of Synaptic Vesicle Pools and Channel-Vesicle Coupling at Hippocampal Mossy Fiber Boutons.” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/AT:ISTA:18296\">https://doi.org/10.15479/AT:ISTA:18296</a>.","apa":"Kim, O. (2024). Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:18296\">https://doi.org/10.15479/AT:ISTA:18296</a>","ieee":"O. Kim, “Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons.” Institute of Science and Technology Austria, 2024.","mla":"Kim, Olena. <i>Presynaptic CAMP-PKA-Mediated Potentiation Induces Reconfiguration of Synaptic Vesicle Pools and Channel-Vesicle Coupling at Hippocampal Mossy Fiber Boutons</i>. Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:18296\">10.15479/AT:ISTA:18296</a>.","short":"O. Kim, (2024).","ama":"Kim O. Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons. 2024. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:18296\">10.15479/AT:ISTA:18296</a>"},"_id":"18296","day":"11","keyword":["Hippocampal mossy fiber synapses","short-term potentiation","long-term potentiation","presynaptic plasticity","electron microscopy","freeze-fracture replica labeling","paired recordings","forskolin","cyclic adenosine monophosphate (cAMP)","protein kinase A (PKA)","neuromodulation","synaptic vesicle pools","presynaptic Ca2+ channels","Munc13","docking","priming","active zone"],"oa_version":"Submitted Version","fulldoi":"https://doi.org/10.15479/AT:ISTA:18296","doi":"10.15479/AT:ISTA:18296","article_processing_charge":"No","type":"research_data","has_accepted_license":"1","project":[{"grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"}],"related_material":{"record":[{"relation":"used_in_publication","id":"18603","status":"public"}]},"month":"10","date_published":"2024-10-11T00:00:00Z","department":[{"_id":"PeJo"},{"_id":"RySh"},{"_id":"EM-Fac"}],"ec_funded":1,"date_created":"2024-10-11T10:12:17Z","file":[{"relation":"main_file","content_type":"application/zip","file_size":164382,"file_id":"18297","creator":"okim","access_level":"open_access","date_updated":"2024-10-11T10:04:19Z","checksum":"0a977e7df54c418251b10dfd3f8a015c","date_created":"2024-10-11T10:04:19Z","file_name":"Kim_et_al_2024_PlosBio_Source_data.zip","success":1},{"checksum":"5b9343d6b2035ac3185e390fad4d3830","date_created":"2024-10-11T10:04:23Z","file_name":"info.txt","success":1,"access_level":"open_access","date_updated":"2024-10-11T10:04:23Z","creator":"okim","file_size":654,"file_id":"18298","relation":"main_file","content_type":"text/plain"}],"date_updated":"2026-09-14T08:48:01Z","contributor":[{"first_name":"Olena","id":"3F8ABDDA-F248-11E8-B48F-1D18A9856A87","contributor_type":"researcher","last_name":"Kim"},{"contributor_type":"researcher","last_name":"Okamoto","first_name":"Yuji","id":"3337E116-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0408-6094"},{"contributor_type":"researcher","last_name":"Kaufmann","orcid":"0000-0001-9735-5315","first_name":"Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Nils ","contributor_type":"researcher","last_name":"Brose"},{"contributor_type":"researcher","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi","orcid":"0000-0001-8761-9444"},{"contributor_type":"supervisor","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M","orcid":"0000-0001-5001-4804"}],"year":"2024","status":"public","ddc":["570"],"file_date_updated":"2024-10-11T10:04:23Z","oa":1,"author":[{"orcid":"0000-0003-2344-1039","id":"3F8ABDDA-F248-11E8-B48F-1D18A9856A87","first_name":"Olena","full_name":"Kim, Olena","last_name":"Kim"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"It is widely believed that information storage in neuronal circuits involves nanoscopic structural changes at synapses, resulting in the formation of synaptic engrams. However, direct evidence for this hypothesis is lacking. To test this conjecture, we combined chemical potentiation, functional analysis by paired pre-postsynaptic recordings, and structural analysis by electron microscopy (EM) and freeze-fracture replica labeling (FRL) at the murine hippocampal mossy fiber synapse, a key synapse in the trisynaptic circuit of the hippocampus. Biophysical analysis of synaptic transmission revealed that forskolin-induced chemical potentiation increased the readily releasable vesicle pool size and vesicular release probability by 146% and 49%, respectively. Structural analysis of mossy fiber synapses by EM and FRL demonstrated an increase in the number of vesicles close to the plasma membrane and the number of clusters of the priming protein Munc13-1, indicating an increase in the number of both docked and primed vesicles. Furthermore, FRL analysis revealed a significant reduction of the distance between Munc13-1 and CaV2.1 Ca2+ channels, suggesting reconfiguration of the channel-vesicle coupling nanotopography. Our results indicate that presynaptic plasticity is associated with structural reorganization of active zones. We propose that changes in potential nanoscopic organization at synaptic vesicle release sites may be correlates of learning and memory at a plastic central synapse."}],"corr_author":"1","publisher":"Institute of Science and Technology Austria"},{"article_number":"A289","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","title":"Binarity at LOw Metallicity (BLOeM): A spectroscopic VLT monitoring survey of massive stars in the SMC","publication_identifier":{"eissn":["1432-0746"],"issn":["0004-6361"]},"publication_status":"published","external_id":{"arxiv":["2407.14593"],"isi":["001336770600014"]},"citation":{"apa":"Shenar, T., Bodensteiner, J., Sana, H., Crowther, P. A., Lennon, D. J., Abdul-Masih, M., … Willcox, R. (2024). Binarity at LOw Metallicity (BLOeM): A spectroscopic VLT monitoring survey of massive stars in the SMC. <i>Astronomy &#38; Astrophysics</i>. EDP Sciences. <a href=\"https://doi.org/10.1051/0004-6361/202451586\">https://doi.org/10.1051/0004-6361/202451586</a>","mla":"Shenar, T., et al. “Binarity at LOw Metallicity (BLOeM): A Spectroscopic VLT Monitoring Survey of Massive Stars in the SMC.” <i>Astronomy &#38; Astrophysics</i>, vol. 690, A289, EDP Sciences, 2024, doi:<a href=\"https://doi.org/10.1051/0004-6361/202451586\">10.1051/0004-6361/202451586</a>.","ieee":"T. Shenar <i>et al.</i>, “Binarity at LOw Metallicity (BLOeM): A spectroscopic VLT monitoring survey of massive stars in the SMC,” <i>Astronomy &#38; Astrophysics</i>, vol. 690. EDP Sciences, 2024.","short":"T. Shenar, J. Bodensteiner, H. Sana, P.A. Crowther, D.J. Lennon, M. Abdul-Masih, L.A. Almeida, F. Backs, S.R. Berlanas, M. Bernini-Peron, J.M. Bestenlehner, D.M. Bowman, V.A. Bronner, N. Britavskiy, A. De Koter, S.E. De Mink, K. Deshmukh, C.J. Evans, M. Fabry, M. Gieles, A. Gilkis, G. González-Torà, G. Gräfener, Y.L.L. Götberg, C. Hawcroft, V. Hénault-Brunet, A. Herrero, G. Holgado, S. Janssens, C. Johnston, J. Josiek, S. Justham, V.M. Kalari, Z.Z. Katabi, Z. Keszthelyi, J. Klencki, J. Kubát, B. Kubátová, N. Langer, R.R. Lefever, B. Ludwig, J. Mackey, L. Mahy, J. Maíz Apellániz, I. Mandel, G. Maravelias, P. Marchant, A. Menon, F. Najarro, L.M. Oskinova, A.J.G. O’Grady, R. Ovadia, L.R. Patrick, D. Pauli, M. Pawlak, V. Ramachandran, M. Renzo, D.F. Rocha, A.A.C. Sander, T. Sayada, F.R.N. Schneider, A. Schootemeijer, E.C. Schösser, C. Schürmann, K. Sen, S. Shahaf, S. Simón-Díaz, M. Stoop, S. Toonen, F. Tramper, J.T. Van Loon, R. Valli, L.A.C. Van Son, A. Vigna-Gómez, J.I. Villaseñor, J.S. Vink, C. Wang, R. Willcox, Astronomy &#38; Astrophysics 690 (2024).","ama":"Shenar T, Bodensteiner J, Sana H, et al. Binarity at LOw Metallicity (BLOeM): A spectroscopic VLT monitoring survey of massive stars in the SMC. <i>Astronomy &#38; Astrophysics</i>. 2024;690. doi:<a href=\"https://doi.org/10.1051/0004-6361/202451586\">10.1051/0004-6361/202451586</a>","ista":"Shenar T, Bodensteiner J, Sana H, Crowther PA, Lennon DJ, Abdul-Masih M, Almeida LA, Backs F, Berlanas SR, Bernini-Peron M, Bestenlehner JM, Bowman DM, Bronner VA, Britavskiy N, De Koter A, De Mink SE, Deshmukh K, Evans CJ, Fabry M, Gieles M, Gilkis A, González-Torà G, Gräfener G, Götberg YLL, Hawcroft C, Hénault-Brunet V, Herrero A, Holgado G, Janssens S, Johnston C, Josiek J, Justham S, Kalari VM, Katabi ZZ, Keszthelyi Z, Klencki J, Kubát J, Kubátová B, Langer N, Lefever RR, Ludwig B, Mackey J, Mahy L, Maíz Apellániz J, Mandel I, Maravelias G, Marchant P, Menon A, Najarro F, Oskinova LM, O’Grady AJG, Ovadia R, Patrick LR, Pauli D, Pawlak M, Ramachandran V, Renzo M, Rocha DF, Sander AAC, Sayada T, Schneider FRN, Schootemeijer A, Schösser EC, Schürmann C, Sen K, Shahaf S, Simón-Díaz S, Stoop M, Toonen S, Tramper F, Van Loon JT, Valli R, Van Son LAC, Vigna-Gómez A, Villaseñor JI, Vink JS, Wang C, Willcox R. 2024. Binarity at LOw Metallicity (BLOeM): A spectroscopic VLT monitoring survey of massive stars in the SMC. Astronomy &#38; Astrophysics. 690, A289.","chicago":"Shenar, T., J. Bodensteiner, H. Sana, P. A. Crowther, D. J. Lennon, M. Abdul-Masih, L. A. Almeida, et al. “Binarity at LOw Metallicity (BLOeM): A Spectroscopic VLT Monitoring Survey of Massive Stars in the SMC.” <i>Astronomy &#38; Astrophysics</i>. EDP Sciences, 2024. <a href=\"https://doi.org/10.1051/0004-6361/202451586\">https://doi.org/10.1051/0004-6361/202451586</a>."},"article_processing_charge":"Yes (in subscription journal)","fulldoi":"https://doi.org/10.1051/0004-6361/202451586","language":[{"iso":"eng"}],"oa_version":"Published Version","day":"01","_id":"18492","doi":"10.1051/0004-6361/202451586","article_type":"original","isi":1,"arxiv":1,"has_accepted_license":"1","type":"journal_article","date_published":"2024-10-01T00:00:00Z","month":"10","OA_place":"publisher","supplementarymaterial":"no","file":[{"file_id":"18500","file_size":4267349,"content_type":"application/pdf","relation":"main_file","file_name":"2024_AstronomyAstrophysics_Shenar.pdf","date_created":"2024-11-04T09:52:26Z","success":1,"checksum":"b378b36726591f3479a927d924ab8e77","date_updated":"2024-11-04T09:52:26Z","access_level":"open_access","creator":"dernst"}],"date_updated":"2026-09-16T10:50:17Z","das_tickbox":"1","department":[{"_id":"YlGo"}],"date_created":"2024-11-03T23:01:44Z","acknowledgement":"The research leading to these results has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement numbers 772225: MULTIPLES). PAC and JMB are supported by the Science and Technology Facilities Council research grant ST/V000853/1 (PI. V. Dhillon). DMB gratefully acknowledges support from UK Research and Innovation (UKRI) in the form of a Frontier Research grant under the UK government’s ERC Horizon Europe funding guarantee (SYMPHONY; PI Bowman; grant number: EP/Y031059/1), and a Royal Society University Research Fellowship (PI Bowman; grant number: URF\\R1\\231631). ZK acknowledges support from JSPS Kakenhi Grant-in-Aid for Scientific Research (23K19071). IM acknowledges support from the Australian Research Council (ARC) Centre of Excellence for Gravitational Wave Discovery (OzGrav), through project number CE230100016. AACS, VR, RRL, and MBP are funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) in the form of an Emmy Noether Research Group – Project-ID 445674056 (SA4064/1-1, PI Sander). GGT and JJ are supported by the German Deutsche Forschungsgemeinschaft (DFG) under Project-ID 496854903 (SA4064/2-1, PI Sander) VR, GGT, and AACS further acknowledge support from the Federal Ministry of Education and Research (BMBF) and the Baden-Württemberg Ministry of Science as part of the Excellence Strategy of the German Federal and State Governments. ECS acknowledges financial support by the Federal Ministry for Economic Affairs and Climate Action (BMWK) via the German Aerospace Center (Deutsches Zentrum für Luft- und Raumfahrt, DLR) grant 50 OR 2306 (PI: Ramachandran/Sander). This work has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement No. 945806) and is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy EXC 2181/1-390900948 (the Heidelberg STRUCTURES Excellence Cluster). LMO is thankful for the funding provided by the DFG grant 443790621. This paper benefited from discussions at the International Space Science Institute (ISSI) in Bern through ISSI International Team project 512 (Multiwavelength View on Massive Stars in the Era of Multimessenger Astronomy). DP acknowledges financial support by the Deutsches Zentrum für Luft und Raumfahrt (DLR) grant FKZ 50OR2005. JIV acknowledges the European Research Council for support from the ERC Advanced grant ERC-2021-ADG101054731. JSV is supported by STFC (Science and Technology Facilities Council) funding under grant number ST/V000233/1. GH, SS-D, SRB and AH acknowledge support from the State Research Agency (AEI) of the Spanish Ministry of Science and Innovation (MICIN) and the European Regional Development Fund, FEDER under grants PID2021-122397NB-C21 and CEX2019-000920-S. SRB also acknowledges financial support by NextGeneration EU/PRTR and MIU (UNI/551/2021) through grant Margarita Salas-ULL. DFR is thankful for the support of the CAPES-Br and FAPERJ/DSC-10 (SEI-260003/001630/2023). F.N., and L.R.P. acknowledge support by grants PID2019-105552RB-C41 and PID2022-137779OB-C41 funded by MCIN/AEI/10.13039/501100011033 by “ERDF A way of making Europe”. MG acknowledges financial support from the grants PID2021-125485NB-C22, CEX2019-000918-M funded by MCIN/AEI/10.13039/501100011033 (State Agency for Research of the Spanish Ministry of Science and Innovation) and SGR-2021-01069 (AGAUR). GM acknowledges funding support from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement No. 772086). JMA acknowledges support from the Spanish Government Ministerio de Ciencia e Innovación and Agencia Estatal de Investigación (10.13 039/501 100 011 033) through grant PID2022-136640 NB-C22 and from the Consejo Superior de Investigaciones Científicas (CSIC) through grant 2022-AEP 005. MP is supported by the BEKKER fellowship BPN/BEK/2022/1/00106 from the Polish National Agency for Academic Exchange. KS is funded by the National Science Center (NCN), Poland, under grant number OPUS 2021/41/B/ST9/00757. JM acknowledges support from a Royal Society-Science Foundation Ireland University Research Fellowship. SJ acknowledges support from the FWO PhD fellowship under project 11E1721N. FB acknowledges the support of the European Research Council (ERC) Horizon Europe under grant agreement number 101044048.","status":"public","OA_type":"hybrid","ddc":["520"],"quality_controlled":"1","scopus_import":"1","intvolume":"       690","publication":"Astronomy & Astrophysics","year":"2024","volume":690,"researchdata_availability":"no","publisher":"EDP Sciences","author":[{"last_name":"Shenar","first_name":"T.","full_name":"Shenar, T."},{"first_name":"J.","full_name":"Bodensteiner, J.","last_name":"Bodensteiner"},{"first_name":"H.","full_name":"Sana, H.","last_name":"Sana"},{"first_name":"P. A.","full_name":"Crowther, P. A.","last_name":"Crowther"},{"full_name":"Lennon, D. J.","first_name":"D. J.","last_name":"Lennon"},{"first_name":"M.","full_name":"Abdul-Masih, M.","last_name":"Abdul-Masih"},{"last_name":"Almeida","full_name":"Almeida, L. A.","first_name":"L. A."},{"full_name":"Backs, F.","first_name":"F.","last_name":"Backs"},{"first_name":"S. R.","full_name":"Berlanas, S. R.","last_name":"Berlanas"},{"last_name":"Bernini-Peron","full_name":"Bernini-Peron, M.","first_name":"M."},{"full_name":"Bestenlehner, J. M.","first_name":"J. M.","last_name":"Bestenlehner"},{"last_name":"Bowman","full_name":"Bowman, D. M.","first_name":"D. M."},{"full_name":"Bronner, V. A.","first_name":"V. A.","last_name":"Bronner"},{"first_name":"N.","full_name":"Britavskiy, N.","last_name":"Britavskiy"},{"last_name":"De Koter","first_name":"A.","full_name":"De Koter, A."},{"first_name":"S. E.","full_name":"De Mink, S. E.","last_name":"De Mink"},{"last_name":"Deshmukh","first_name":"K.","full_name":"Deshmukh, K."},{"last_name":"Evans","first_name":"C. J.","full_name":"Evans, C. J."},{"last_name":"Fabry","first_name":"M.","full_name":"Fabry, M."},{"last_name":"Gieles","first_name":"M.","full_name":"Gieles, M."},{"full_name":"Gilkis, A.","first_name":"A.","last_name":"Gilkis"},{"last_name":"González-Torà","full_name":"González-Torà, G.","first_name":"G."},{"last_name":"Gräfener","first_name":"G.","full_name":"Gräfener, G."},{"full_name":"Götberg, Ylva Louise Linsdotter","orcid":"0000-0002-6960-6911","id":"d0648d0c-0f64-11ee-a2e0-dd0faa2e4f7d","first_name":"Ylva Louise Linsdotter","last_name":"Götberg"},{"last_name":"Hawcroft","full_name":"Hawcroft, C.","first_name":"C."},{"full_name":"Hénault-Brunet, V.","first_name":"V.","last_name":"Hénault-Brunet"},{"full_name":"Herrero, A.","first_name":"A.","last_name":"Herrero"},{"last_name":"Holgado","full_name":"Holgado, G.","first_name":"G."},{"full_name":"Janssens, S.","first_name":"S.","last_name":"Janssens"},{"last_name":"Johnston","first_name":"C.","full_name":"Johnston, C."},{"first_name":"J.","full_name":"Josiek, J.","last_name":"Josiek"},{"last_name":"Justham","full_name":"Justham, S.","first_name":"S."},{"first_name":"V. M.","full_name":"Kalari, V. M.","last_name":"Kalari"},{"last_name":"Katabi","first_name":"Z. Z.","full_name":"Katabi, Z. Z."},{"last_name":"Keszthelyi","first_name":"Z.","full_name":"Keszthelyi, Z."},{"last_name":"Klencki","first_name":"J.","full_name":"Klencki, J."},{"last_name":"Kubát","full_name":"Kubát, J.","first_name":"J."},{"last_name":"Kubátová","full_name":"Kubátová, B.","first_name":"B."},{"last_name":"Langer","full_name":"Langer, N.","first_name":"N."},{"first_name":"R. R.","full_name":"Lefever, R. R.","last_name":"Lefever"},{"full_name":"Ludwig, B.","first_name":"B.","last_name":"Ludwig"},{"first_name":"J.","full_name":"Mackey, J.","last_name":"Mackey"},{"full_name":"Mahy, L.","first_name":"L.","last_name":"Mahy"},{"last_name":"Maíz Apellániz","first_name":"J.","full_name":"Maíz Apellániz, J."},{"last_name":"Mandel","full_name":"Mandel, I.","first_name":"I."},{"last_name":"Maravelias","first_name":"G.","full_name":"Maravelias, G."},{"last_name":"Marchant","full_name":"Marchant, P.","first_name":"P."},{"full_name":"Menon, A.","first_name":"A.","last_name":"Menon"},{"last_name":"Najarro","full_name":"Najarro, F.","first_name":"F."},{"first_name":"L. M.","full_name":"Oskinova, L. M.","last_name":"Oskinova"},{"full_name":"O'Grady, A. J.G.","first_name":"A. J.G.","last_name":"O'Grady"},{"first_name":"R.","full_name":"Ovadia, R.","last_name":"Ovadia"},{"last_name":"Patrick","full_name":"Patrick, L. R.","first_name":"L. R."},{"last_name":"Pauli","full_name":"Pauli, D.","first_name":"D."},{"last_name":"Pawlak","full_name":"Pawlak, M.","first_name":"M."},{"first_name":"V.","full_name":"Ramachandran, V.","last_name":"Ramachandran"},{"full_name":"Renzo, M.","first_name":"M.","last_name":"Renzo"},{"first_name":"D. F.","full_name":"Rocha, D. F.","last_name":"Rocha"},{"full_name":"Sander, A. A.C.","first_name":"A. A.C.","last_name":"Sander"},{"last_name":"Sayada","full_name":"Sayada, T.","first_name":"T."},{"last_name":"Schneider","full_name":"Schneider, F. R.N.","first_name":"F. R.N."},{"first_name":"A.","full_name":"Schootemeijer, A.","last_name":"Schootemeijer"},{"last_name":"Schösser","full_name":"Schösser, E. C.","first_name":"E. C."},{"last_name":"Schürmann","full_name":"Schürmann, C.","first_name":"C."},{"last_name":"Sen","full_name":"Sen, K.","first_name":"K."},{"first_name":"S.","full_name":"Shahaf, S.","last_name":"Shahaf"},{"first_name":"S.","full_name":"Simón-Díaz, S.","last_name":"Simón-Díaz"},{"last_name":"Stoop","first_name":"M.","full_name":"Stoop, M."},{"full_name":"Toonen, S.","first_name":"S.","last_name":"Toonen"},{"first_name":"F.","full_name":"Tramper, F.","last_name":"Tramper"},{"last_name":"Van Loon","full_name":"Van Loon, J. Th","first_name":"J. Th"},{"last_name":"Valli","first_name":"R.","full_name":"Valli, R."},{"full_name":"Van Son, L. A.C.","first_name":"L. A.C.","last_name":"Van Son"},{"first_name":"A.","full_name":"Vigna-Gómez, A.","last_name":"Vigna-Gómez"},{"last_name":"Villaseñor","first_name":"J. I.","full_name":"Villaseñor, J. I."},{"full_name":"Vink, J. S.","first_name":"J. S.","last_name":"Vink"},{"first_name":"C.","full_name":"Wang, C.","last_name":"Wang"},{"last_name":"Willcox","full_name":"Willcox, R.","first_name":"R."}],"file_date_updated":"2024-11-04T09:52:26Z","oa":1,"abstract":[{"lang":"eng","text":"Surveys in the Milky Way and Large Magellanic Cloud have revealed that the majority of massive stars will interact with companions during their lives. However, knowledge of the binary properties of massive stars at low metallicity, and therefore in conditions approaching those of the Early Universe, remain sparse. We present the Binarity at LOw Metallicity (BLOeM) campaign, an ESO large programme designed to obtain 25 epochs of spectroscopy for 929 massive stars in the Small Magellanic Cloud, allowing us to probe multiplicity in the lowest-metallicity conditions to date (Z = 0.2 Z⊙). BLOeM will provide (i) the binary fraction, (ii) the orbital configurations of systems with periods of P ≲ 3 yr, (iii) dormant black-hole binary candidates (OB+BH), and (iv) a legacy database of physical parameters of massive stars at low metallicity. Main sequence (OB-type) and evolved (OBAF-type) massive stars are observed with the LR02 setup of the GIRAFFE instrument of the Very Large Telescope (3960–4570 Å resolving power R = 6200; typical signal-to-noise ratio(S/N) ≈70–100). This paper utilises the first nine epochs obtained over a three-month time period. We describe the survey and data reduction, perform a spectral classification of the stacked spectra, and construct a Hertzsprung-Russell diagram of the sample via spectral-type and photometric calibrations. Our detailed classification reveals that the sample covers spectral types from O4 to F5, spanning the effective temperature and luminosity ranges 6.5 ≲ Teff/kK ≲ 45 and 3.7 < log L/L⊙ < 6.1 and initial masses of 8 ≲ Mini ≲ 80 M⊙. The sample comprises 159 O-type stars, 331 early B-type (B0–3) dwarfs and giants (luminosity classes V–III), 303 early B-type supergiants (II–I), and 136 late-type BAF supergiants. At least 82 stars are OBe stars: 20 O-type and 62 B-type (13% and 11% of the respective samples). In addition, the sample includes 4 high-mass X-ray binaries, 3 stars resembling luminous blue variables, 2 bloated stripped-star candidates, 2 candidate magnetic stars, and 74 eclipsing binaries."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"}},{"intvolume":"         6","ddc":["530"],"status":"public","quality_controlled":"1","OA_type":"gold","DOAJ_listed":"1","scopus_import":"1","researchdata_availability":"no","APC_amount":"2933,65 EUR","publication":"Physical Review Research","year":"2024","volume":6,"publisher":"American Physical Society","abstract":[{"lang":"eng","text":"Precision sensing and manipulation of milligram-scale mechanical oscillators has attracted growing interest in the fields of table-top explorations of gravity and tests of quantum mechanics at macroscopic scales. Torsional oscillators present an opportunity in this regard due to their remarked isolation from environmental noise. For torsional motion, an effective employment of optical cavities to enhance optomechanical interactions—as already established for linear oscillators—so far faced certain challenges. Here, we propose a concept for sensing and manipulating torsional motion, where exclusively the torsional rotations of a pendulum are mapped onto the path length of a single two-mirror optical cavity. The concept inherently alleviates many limitations of previous approaches. A proof-of-principle experiment is conducted with a rigidly controlled pendulum to explore the sensing aspects of the concept and to identify practical limitations in a potential state-of-the art setup. Based on this study, we anticipate development of precision torque sensors utilizing torsional pendulums that can support sensitivities below 10−19Nm/√Hz, while the motion of the pendulums are dominated by quantum radiation pressure noise at sub-microwatts of incoming laser power. These developments will provide horizons for experiments at the interface of quantum mechanics and gravity."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"corr_author":"1","author":[{"last_name":"Agafonova","orcid":"0000-0003-0582-2946","first_name":"Sofya","id":"09501ff6-dca7-11ea-a8ae-b3e0b9166e80","full_name":"Agafonova, Sofya"},{"id":"4328fa4c-f128-11eb-9611-c107b0fe4d51","first_name":"Umang","full_name":"Mishra, Umang","last_name":"Mishra"},{"orcid":"0000-0002-4947-8924","id":"2E054C4C-F248-11E8-B48F-1D18A9856A87","first_name":"Fritz R","full_name":"Diorico, Fritz R","last_name":"Diorico"},{"last_name":"Hosten","orcid":"0000-0002-2031-204X","first_name":"Onur","id":"4C02D85E-F248-11E8-B48F-1D18A9856A87","full_name":"Hosten, Onur"}],"oa":1,"file_date_updated":"2024-02-12T11:46:50Z","date_published":"2024-02-05T00:00:00Z","OA_place":"publisher","supplementarymaterial":"no","month":"02","date_updated":"2026-09-17T09:13:08Z","das_tickbox":"0","file":[{"content_type":"application/pdf","relation":"main_file","file_size":1437167,"file_id":"14981","access_level":"open_access","date_updated":"2024-02-12T11:46:50Z","creator":"dernst","checksum":"3a39ebffb24c1cc1dd0b547a726dc52d","date_created":"2024-02-12T11:46:50Z","success":1,"file_name":"2024_PhysicalRevResearch_Agafonova.pdf"}],"date_created":"2024-02-12T11:42:18Z","acknowledgement":"We thank Pere Rosselló for his contributions to the initial modeling of the presented sensing technique. This work was supported by Institute of Science and Technology Austria, and\r\nthe European Research Council under Grant No. 101087907 (ERC CoG QuHAMP).","department":[{"_id":"OnHo"}],"article_processing_charge":"Yes","doi":"10.1103/physrevresearch.6.013141","language":[{"iso":"eng"}],"fulldoi":"https://doi.org/10.1103/physrevresearch.6.013141","oa_version":"Published Version","day":"05","_id":"14980","issue":"1","arxiv":1,"article_type":"original","project":[{"grant_number":"101087907","name":"A quantum hybrid of atoms and milligram-scale pendulums: towards gravitational quantum mechanics","_id":"bdb2a702-d553-11ed-ba76-f12e3e5a3bc6"}],"has_accepted_license":"1","type":"journal_article","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"013141","publication_identifier":{"eissn":["2643-1564"]},"publication_status":"published","external_id":{"arxiv":["2306.12804"]},"title":"Zigzag optical cavity for sensing and controlling torsional motion","citation":{"mla":"Agafonova, Sofia, et al. “Zigzag Optical Cavity for Sensing and Controlling Torsional Motion.” <i>Physical Review Research</i>, vol. 6, no. 1, 013141, American Physical Society, 2024, doi:<a href=\"https://doi.org/10.1103/physrevresearch.6.013141\">10.1103/physrevresearch.6.013141</a>.","ieee":"S. Agafonova, U. Mishra, F. R. Diorico, and O. Hosten, “Zigzag optical cavity for sensing and controlling torsional motion,” <i>Physical Review Research</i>, vol. 6, no. 1. American Physical Society, 2024.","ama":"Agafonova S, Mishra U, Diorico FR, Hosten O. Zigzag optical cavity for sensing and controlling torsional motion. <i>Physical Review Research</i>. 2024;6(1). doi:<a href=\"https://doi.org/10.1103/physrevresearch.6.013141\">10.1103/physrevresearch.6.013141</a>","short":"S. Agafonova, U. Mishra, F.R. Diorico, O. Hosten, Physical Review Research 6 (2024).","apa":"Agafonova, S., Mishra, U., Diorico, F. R., &#38; Hosten, O. (2024). Zigzag optical cavity for sensing and controlling torsional motion. <i>Physical Review Research</i>. American Physical Society. <a href=\"https://doi.org/10.1103/physrevresearch.6.013141\">https://doi.org/10.1103/physrevresearch.6.013141</a>","ista":"Agafonova S, Mishra U, Diorico FR, Hosten O. 2024. Zigzag optical cavity for sensing and controlling torsional motion. Physical Review Research. 6(1), 013141.","chicago":"Agafonova, Sofia, Umang Mishra, Fritz R Diorico, and Onur Hosten. “Zigzag Optical Cavity for Sensing and Controlling Torsional Motion.” <i>Physical Review Research</i>. American Physical Society, 2024. <a href=\"https://doi.org/10.1103/physrevresearch.6.013141\">https://doi.org/10.1103/physrevresearch.6.013141</a>."}},{"month":"07","supplementarymaterial":"no","OA_place":"publisher","date_published":"2024-07-01T00:00:00Z","department":[{"_id":"PaSc"}],"acknowledgement":"G.H. is the incumbent of the Hilda Pomeraniec Memorial Professorial Chair. He has been partially funded by the European Research Council under the European Union's Horizon 2020 research and innovation program (grant 742637, SMALLOSTERY), by National Science Foundation–US-Israel Binational Science Foundation grant 2021700, and by an Israel Science Foundation Breakthrough grant (1924/22). P.S. acknowledges funding from the Austrian Science Fund (project “AlloSpace,” I05812) and intramural funding from the Institute of Science and Technology Austria.","date_created":"2025-01-27T13:40:34Z","file":[{"date_created":"2025-01-27T13:44:59Z","success":1,"file_name":"2024_AnnualReviews_Schanda.pdf","checksum":"c90861542ae3f9147939030d5bafed3c","creator":"dernst","date_updated":"2025-01-27T13:44:59Z","access_level":"open_access","file_id":"18911","file_size":3025589,"content_type":"application/pdf","relation":"main_file"}],"das_tickbox":"0","date_updated":"2026-09-17T09:44:33Z","volume":53,"year":"2024","publication":"Annual Review of Biophysics","researchdata_availability":"no","pmid":1,"scopus_import":"1","quality_controlled":"1","ddc":["570"],"OA_type":"hybrid","status":"public","intvolume":"        53","oa":1,"file_date_updated":"2025-01-27T13:44:59Z","author":[{"full_name":"Schanda, Paul","orcid":"0000-0002-9350-7606","first_name":"Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","last_name":"Schanda"},{"last_name":"Haran","full_name":"Haran, Gilad","first_name":"Gilad"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"corr_author":"1","abstract":[{"lang":"eng","text":"Proteins often undergo large-scale conformational transitions, in which secondary and tertiary structure elements (loops, helices, and domains) change their structures or their positions with respect to each other. Simple considerations suggest that such dynamics should be relatively fast, but the functional cycles of many proteins are often relatively slow. Sophisticated experimental methods are starting to tackle this dichotomy and shed light on the contribution of large-scale conformational dynamics to protein function. In this review, we focus on the contribution of single-molecule Förster resonance energy transfer and nuclear magnetic resonance (NMR) spectroscopies to the study of conformational dynamics. We briefly describe the state of the art in each of these techniques and then point out their similarities and differences, as well as the relative strengths and weaknesses of each. Several case studies, in which the connection between fast conformational dynamics and slower function has been demonstrated, are then introduced and discussed. These examples include both enzymes and large protein machines, some of which have been studied by both NMR and fluorescence spectroscopies."}],"publisher":"Annual Reviews","title":"NMR and single-molecule FRET insights into fast protein motions and their relation to function","external_id":{"isi":["001278237500012"],"pmid":["38346243"]},"publication_status":"published","publication_identifier":{"issn":["1936-122X"],"eissn":["1936-1238"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"short":"P. Schanda, G. Haran, Annual Review of Biophysics 53 (2024) 247–273.","ama":"Schanda P, Haran G. NMR and single-molecule FRET insights into fast protein motions and their relation to function. <i>Annual Review of Biophysics</i>. 2024;53:247-273. doi:<a href=\"https://doi.org/10.1146/annurev-biophys-070323-022428\">10.1146/annurev-biophys-070323-022428</a>","ieee":"P. Schanda and G. Haran, “NMR and single-molecule FRET insights into fast protein motions and their relation to function,” <i>Annual Review of Biophysics</i>, vol. 53. Annual Reviews, pp. 247–273, 2024.","mla":"Schanda, Paul, and Gilad Haran. “NMR and Single-Molecule FRET Insights into Fast Protein Motions and Their Relation to Function.” <i>Annual Review of Biophysics</i>, vol. 53, Annual Reviews, 2024, pp. 247–73, doi:<a href=\"https://doi.org/10.1146/annurev-biophys-070323-022428\">10.1146/annurev-biophys-070323-022428</a>.","apa":"Schanda, P., &#38; Haran, G. (2024). NMR and single-molecule FRET insights into fast protein motions and their relation to function. <i>Annual Review of Biophysics</i>. Annual Reviews. <a href=\"https://doi.org/10.1146/annurev-biophys-070323-022428\">https://doi.org/10.1146/annurev-biophys-070323-022428</a>","chicago":"Schanda, Paul, and Gilad Haran. “NMR and Single-Molecule FRET Insights into Fast Protein Motions and Their Relation to Function.” <i>Annual Review of Biophysics</i>. Annual Reviews, 2024. <a href=\"https://doi.org/10.1146/annurev-biophys-070323-022428\">https://doi.org/10.1146/annurev-biophys-070323-022428</a>.","ista":"Schanda P, Haran G. 2024. NMR and single-molecule FRET insights into fast protein motions and their relation to function. Annual Review of Biophysics. 53, 247–273."},"_id":"18910","day":"01","language":[{"iso":"eng"}],"fulldoi":"https://doi.org/10.1146/annurev-biophys-070323-022428","oa_version":"Published Version","doi":"10.1146/annurev-biophys-070323-022428","article_processing_charge":"No","type":"journal_article","project":[{"_id":"eb9c82eb-77a9-11ec-83b8-aadd536561cf","name":"AlloSpace. The emergence and mechanisms of allostery","grant_number":"I05812"}],"has_accepted_license":"1","article_type":"original","page":"247-273","isi":1},{"date_published":"2024-03-08T00:00:00Z","supplementarymaterial":"no","month":"03","das_tickbox":"0","date_updated":"2026-09-17T09:49:03Z","ec_funded":1,"acknowledgement":"We thank previous students, postdocs, and collaborators, particularly J. Geiger, and (in alphabetical order) H. Alle, J. Bischofberger, C. Borges-Merjane, D. Engel, M. Frotscher, S. Hallermann, M. Heckmann, S. Jamrichova, O. Kim, L. Li, K. Lichter, P. Lin, J. Lübke, Y. Okamoto, C. Pawlu, C. Schmidt-Hieber, N. Spruston, and N. Vyleta for their outstanding experimental contributions. We also thank P. Castillo, J. Geiger, T. Sakaba, S. Siegert, T. Vogels, and J. Watson for critically reading the manuscript, E. Kralli-Beller for text editing, and J. Malikovic and L. Slomianka for useful discussions. We apologize that, due to space constraints, not all relevant papers could be cited.\r\nThis project was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement 692692, AdG “GIANTSYN”) and the Fonds zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein Award; P 36232-B, stand-alone grant), both to P.J.","date_created":"2024-03-17T23:00:57Z","department":[{"_id":"PeJo"}],"intvolume":"       383","scopus_import":"1","pmid":1,"status":"public","quality_controlled":"1","researchdata_availability":"no","volume":383,"year":"2024","publication":"Science","publisher":"AAAS","abstract":[{"lang":"eng","text":"The hippocampal mossy fiber synapse, formed between axons of dentate gyrus granule cells and dendrites of CA3 pyramidal neurons, is a key synapse in the trisynaptic circuitry of the hippocampus. Because of its comparatively large size, this synapse is accessible to direct presynaptic recording, allowing a rigorous investigation of the biophysical mechanisms of synaptic transmission and plasticity. Furthermore, because of its placement in the very center of the hippocampal memory circuit, this synapse seems to be critically involved in several higher network functions, such as learning, memory, pattern separation, and pattern completion. Recent work based on new technologies in both nanoanatomy and nanophysiology, including presynaptic patch-clamp recording, paired recording, super-resolution light microscopy, and freeze-fracture and “flash-and-freeze” electron microscopy, has provided new insights into the structure, biophysics, and network function of this intriguing synapse. This brings us one step closer to answering a fundamental question in neuroscience: how basic synaptic properties shape higher network computations."}],"corr_author":"1","author":[{"orcid":"0000-0001-7577-1676","id":"3AE48E0A-F248-11E8-B48F-1D18A9856A87","first_name":"David H","full_name":"Vandael, David H","last_name":"Vandael"},{"last_name":"Jonas","full_name":"Jonas, Peter M","first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","external_id":{"isi":["001216996700015"],"pmid":["38452088"]},"publication_status":"published","publication_identifier":{"eissn":["1095-9203"]},"title":"Structure, biophysics, and circuit function of a \"giant\" cortical presynaptic terminal","citation":{"chicago":"Vandael, David H, and Peter M Jonas. “Structure, Biophysics, and Circuit Function of a ‘Giant’ Cortical Presynaptic Terminal.” <i>Science</i>. AAAS, 2024. <a href=\"https://doi.org/10.1126/science.adg6757\">https://doi.org/10.1126/science.adg6757</a>.","ista":"Vandael DH, Jonas PM. 2024. Structure, biophysics, and circuit function of a ‘giant’ cortical presynaptic terminal. Science. 383(6687), eadg6757.","ama":"Vandael DH, Jonas PM. Structure, biophysics, and circuit function of a “giant” cortical presynaptic terminal. <i>Science</i>. 2024;383(6687):eadg6757. doi:<a href=\"https://doi.org/10.1126/science.adg6757\">10.1126/science.adg6757</a>","short":"D.H. Vandael, P.M. Jonas, Science 383 (2024) eadg6757.","ieee":"D. H. Vandael and P. M. Jonas, “Structure, biophysics, and circuit function of a ‘giant’ cortical presynaptic terminal,” <i>Science</i>, vol. 383, no. 6687. AAAS, p. eadg6757, 2024.","mla":"Vandael, David H., and Peter M. Jonas. “Structure, Biophysics, and Circuit Function of a ‘Giant’ Cortical Presynaptic Terminal.” <i>Science</i>, vol. 383, no. 6687, AAAS, 2024, p. eadg6757, doi:<a href=\"https://doi.org/10.1126/science.adg6757\">10.1126/science.adg6757</a>.","apa":"Vandael, D. H., &#38; Jonas, P. M. (2024). Structure, biophysics, and circuit function of a “giant” cortical presynaptic terminal. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adg6757\">https://doi.org/10.1126/science.adg6757</a>"},"article_processing_charge":"No","doi":"10.1126/science.adg6757","day":"08","_id":"15117","language":[{"iso":"eng"}],"fulldoi":"https://doi.org/10.1126/science.adg6757","oa_version":"None","isi":1,"issue":"6687","article_type":"review","page":"eadg6757","type":"journal_article","project":[{"name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","grant_number":"692692","call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"},{"name":"Synaptic communication in neuronal microcircuits","grant_number":"Z00312","_id":"25C5A090-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"grant_number":"P36232","name":"Mechanisms of GABA release in hippocampal circuits","_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5"}]},{"citation":{"ista":"Watson J, Arroyo-Urea S, García-Nafría J. 2024.DNA Cloning. In: Handbook of Molecular Biotechnology. , 66–72.","chicago":"Watson, Jake, Sandra Arroyo-Urea, and Javier García-Nafría. “DNA Cloning.” In <i>Handbook of Molecular Biotechnology</i>, edited by Dongyou Liu, 1st ed., 66–72. Boca Raton: CRC Press, 2024. <a href=\"https://doi.org/10.1201/9781003055211-8\">https://doi.org/10.1201/9781003055211-8</a>.","apa":"Watson, J., Arroyo-Urea, S., &#38; García-Nafría, J. (2024). DNA Cloning. In D. Liu (Ed.), <i>Handbook of Molecular Biotechnology</i> (1st ed., pp. 66–72). Boca Raton: CRC Press. <a href=\"https://doi.org/10.1201/9781003055211-8\">https://doi.org/10.1201/9781003055211-8</a>","mla":"Watson, Jake, et al. “DNA Cloning.” <i>Handbook of Molecular Biotechnology</i>, edited by Dongyou Liu, 1st ed., CRC Press, 2024, pp. 66–72, doi:<a href=\"https://doi.org/10.1201/9781003055211-8\">10.1201/9781003055211-8</a>.","ieee":"J. Watson, S. Arroyo-Urea, and J. García-Nafría, “DNA Cloning,” in <i>Handbook of Molecular Biotechnology</i>, 1st ed., D. Liu, Ed. Boca Raton: CRC Press, 2024, pp. 66–72.","short":"J. Watson, S. Arroyo-Urea, J. García-Nafría, in:, D. Liu (Ed.), Handbook of Molecular Biotechnology, 1st ed., CRC Press, Boca Raton, 2024, pp. 66–72.","ama":"Watson J, Arroyo-Urea S, García-Nafría J. DNA Cloning. In: Liu D, ed. <i>Handbook of Molecular Biotechnology</i>. 1st ed. Boca Raton: CRC Press; 2024:66-72. doi:<a href=\"https://doi.org/10.1201/9781003055211-8\">10.1201/9781003055211-8</a>"},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_identifier":{"eisbn":["9781003055211"]},"publication_status":"published","title":"DNA Cloning","page":"66-72","type":"book_chapter","article_processing_charge":"No","doi":"10.1201/9781003055211-8","_id":"18058","day":"05","language":[{"iso":"eng"}],"fulldoi":"https://doi.org/10.1201/9781003055211-8","oa_version":"None","das_tickbox":"0","date_updated":"2026-09-17T09:58:21Z","date_created":"2024-09-11T10:40:36Z","editor":[{"last_name":"Liu","first_name":"Dongyou","full_name":"Liu, Dongyou"}],"department":[{"_id":"PeJo"}],"date_published":"2024-09-05T00:00:00Z","edition":"1","supplementarymaterial":"no","month":"09","publisher":"CRC Press","abstract":[{"text":"DNA cloning is a core technique in biomedical and biotechnological research and is used to assemble and modify DNA fragments at will. While DNA cloning has traditionally relied on restriction enzymes, recent homology-based methods offer improved protocols together with seamless and directional assembly of desired products, overcoming the main disadvantages of restriction enzyme DNA cloning. This chapter provides a historical perspective on DNA cloning, presents a detailed discussion on state-of-the-art in vitro and in vivo homology-based methodologies, covering the basics of how to perform all major plasmid modifications (sub-cloning, site-directed mutagenesis, insertions, and deletions), and gives examples of how to apply these techniques for complex DNA cloning projects.","lang":"eng"}],"author":[{"first_name":"Jake","id":"63836096-4690-11EA-BD4E-32803DDC885E","orcid":"0000-0002-8698-3823","full_name":"Watson, Jake","last_name":"Watson"},{"last_name":"Arroyo-Urea","full_name":"Arroyo-Urea, Sandra","first_name":"Sandra"},{"last_name":"García-Nafría","first_name":"Javier","full_name":"García-Nafría, Javier"}],"scopus_import":"1","quality_controlled":"1","status":"public","researchdata_availability":"no","year":"2024","place":"Boca Raton","publication":"Handbook of Molecular Biotechnology"},{"oa":1,"file_date_updated":"2024-12-03T14:07:04Z","author":[{"last_name":"Lauwereyns","first_name":"Jan","full_name":"Lauwereyns, Jan"},{"full_name":"Bajramovic, Jeffrey","first_name":"Jeffrey","last_name":"Bajramovic"},{"first_name":"Bettina","full_name":"Bert, Bettina","last_name":"Bert"},{"last_name":"Camenzind","full_name":"Camenzind, Samuel","first_name":"Samuel"},{"last_name":"De Kock","first_name":"Joery","full_name":"De Kock, Joery"},{"full_name":"Elezović, Alisa","first_name":"Alisa","last_name":"Elezović"},{"full_name":"Erden, Sevilay","first_name":"Sevilay","last_name":"Erden"},{"first_name":"Fernando","full_name":"Gonzalez-Uarquin, Fernando","last_name":"Gonzalez-Uarquin"},{"full_name":"Ulman, Yesim Isil","first_name":"Yesim Isil","last_name":"Ulman"},{"last_name":"Hoffmann","full_name":"Hoffmann, Orsolya Ivett","first_name":"Orsolya Ivett"},{"last_name":"Kitsara","full_name":"Kitsara, Maria","first_name":"Maria"},{"full_name":"Kostomitsopoulos, Nikolaos","first_name":"Nikolaos","last_name":"Kostomitsopoulos"},{"first_name":"Winfried","full_name":"Neuhaus, Winfried","last_name":"Neuhaus"},{"last_name":"Petit-Demouliere","first_name":"Benoit","full_name":"Petit-Demouliere, Benoit"},{"last_name":"Pollo","first_name":"Simone","full_name":"Pollo, Simone"},{"last_name":"Riso","full_name":"Riso, Brígida","first_name":"Brígida"},{"last_name":"Schober","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","first_name":"Sophie","full_name":"Schober, Sophie"},{"first_name":"Athanassia","full_name":"Sotiropoulos, Athanassia","last_name":"Sotiropoulos"},{"last_name":"Thomas","full_name":"Thomas, Aurélie","first_name":"Aurélie"},{"first_name":"Augusto","full_name":"Vitale, Augusto","last_name":"Vitale"},{"full_name":"Wilflingseder, Doris","first_name":"Doris","last_name":"Wilflingseder"},{"full_name":"Ahluwalia, Arti","first_name":"Arti","last_name":"Ahluwalia"}],"abstract":[{"text":"Many scientific breakthroughs have depended on animal research, yet the ethical concerns surrounding the use of animals in experimentation have long prompted discussions about humane treatment and responsible scientific practice. First articulated by Russell and Burch, the 3Rs Principles of Replacement, Reduction, and Refinement have gained widespread recognition as basic guidelines for animal research. Over time, the 3Rs have transcended the research community, influencing policy decisions, animal welfare advocacy and public perception of animal experimentation. Despite their broad acceptance, interpretations of the 3Rs vary substantially, shaping statutory frameworks at various levels, with both technical and practical impacts.","lang":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"publisher":"Springer Nature","volume":53,"year":"2024","publication":"Lab Animal","researchdata_availability":"no","scopus_import":"1","pmid":1,"OA_type":"hybrid","status":"public","quality_controlled":"1","ddc":["570"],"intvolume":"        53","department":[{"_id":"PreCl"}],"acknowledgement":"This publication is based upon work from the Ethics Crossover Group within the COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”), CA21139, supported by COST (European Cooperation in Science and Technology). We acknowledge the input and advice from Dr. Susanna Louhimies.","date_created":"2024-11-24T23:01:49Z","file":[{"file_id":"18614","file_size":967252,"content_type":"application/pdf","relation":"main_file","file_name":"2024_LabAnimal_Lauwereyns.pdf","success":1,"date_created":"2024-12-03T14:07:04Z","checksum":"67fc140f761581a291591f075e49b88d","creator":"dernst","date_updated":"2024-12-03T14:07:04Z","access_level":"open_access"}],"das_tickbox":"0","date_updated":"2026-09-17T10:29:32Z","month":"12","supplementarymaterial":"no","OA_place":"publisher","date_published":"2024-12-01T00:00:00Z","type":"journal_article","has_accepted_license":"1","article_type":"letter_note","page":"347-350","isi":1,"day":"01","_id":"18587","fulldoi":"https://doi.org/10.1038/s41684-024-01476-2","oa_version":"Published Version","language":[{"iso":"eng"}],"doi":"10.1038/s41684-024-01476-2","article_processing_charge":"No","citation":{"short":"J. Lauwereyns, J. Bajramovic, B. Bert, S. Camenzind, J. De Kock, A. Elezović, S. Erden, F. Gonzalez-Uarquin, Y.I. Ulman, O.I. Hoffmann, M. Kitsara, N. Kostomitsopoulos, W. Neuhaus, B. Petit-Demouliere, S. Pollo, B. Riso, S. Schober, A. Sotiropoulos, A. Thomas, A. Vitale, D. Wilflingseder, A. Ahluwalia, Lab Animal 53 (2024) 347–350.","ama":"Lauwereyns J, Bajramovic J, Bert B, et al. Toward a common interpretation of the 3Rs principles in animal research. <i>Lab Animal</i>. 2024;53:347-350. doi:<a href=\"https://doi.org/10.1038/s41684-024-01476-2\">10.1038/s41684-024-01476-2</a>","mla":"Lauwereyns, Jan, et al. “Toward a Common Interpretation of the 3Rs Principles in Animal Research.” <i>Lab Animal</i>, vol. 53, Springer Nature, 2024, pp. 347–50, doi:<a href=\"https://doi.org/10.1038/s41684-024-01476-2\">10.1038/s41684-024-01476-2</a>.","ieee":"J. Lauwereyns <i>et al.</i>, “Toward a common interpretation of the 3Rs principles in animal research,” <i>Lab Animal</i>, vol. 53. Springer Nature, pp. 347–350, 2024.","apa":"Lauwereyns, J., Bajramovic, J., Bert, B., Camenzind, S., De Kock, J., Elezović, A., … Ahluwalia, A. (2024). Toward a common interpretation of the 3Rs principles in animal research. <i>Lab Animal</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41684-024-01476-2\">https://doi.org/10.1038/s41684-024-01476-2</a>","chicago":"Lauwereyns, Jan, Jeffrey Bajramovic, Bettina Bert, Samuel Camenzind, Joery De Kock, Alisa Elezović, Sevilay Erden, et al. “Toward a Common Interpretation of the 3Rs Principles in Animal Research.” <i>Lab Animal</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41684-024-01476-2\">https://doi.org/10.1038/s41684-024-01476-2</a>.","ista":"Lauwereyns J, Bajramovic J, Bert B, Camenzind S, De Kock J, Elezović A, Erden S, Gonzalez-Uarquin F, Ulman YI, Hoffmann OI, Kitsara M, Kostomitsopoulos N, Neuhaus W, Petit-Demouliere B, Pollo S, Riso B, Schober S, Sotiropoulos A, Thomas A, Vitale A, Wilflingseder D, Ahluwalia A. 2024. Toward a common interpretation of the 3Rs principles in animal research. Lab Animal. 53, 347–350."},"title":"Toward a common interpretation of the 3Rs principles in animal research","external_id":{"pmid":["39548348"],"isi":["001355264100001"]},"publication_status":"published","publication_identifier":{"issn":["0093-7355"],"eissn":["1548-4475"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"doi":"10.1038/s41467-024-50587-4","fulldoi":"https://doi.org/10.1038/s41467-024-50587-4","oa_version":"Published Version","language":[{"iso":"eng"}],"day":"31","_id":"17409","article_processing_charge":"Yes","has_accepted_license":"1","type":"journal_article","isi":1,"article_type":"original","publication_status":"published","publication_identifier":{"eissn":["2041-1723"]},"external_id":{"pmid":["39085193"],"isi":["001282384300020"]},"title":"All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors","article_number":"6461","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"ama":"Van Vliet S, Sheng J, Stindt CN, Feringa BL. All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors. <i>Nature Communications</i>. 2024;15. doi:<a href=\"https://doi.org/10.1038/s41467-024-50587-4\">10.1038/s41467-024-50587-4</a>","short":"S. Van Vliet, J. Sheng, C.N. Stindt, B.L. Feringa, Nature Communications 15 (2024).","mla":"Van Vliet, Sven, et al. “All-Visible-Light-Driven Salicylidene Schiff-Base-Functionalized Artificial Molecular Motors.” <i>Nature Communications</i>, vol. 15, 6461, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1038/s41467-024-50587-4\">10.1038/s41467-024-50587-4</a>.","ieee":"S. Van Vliet, J. Sheng, C. N. Stindt, and B. L. Feringa, “All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors,” <i>Nature Communications</i>, vol. 15. Springer Nature, 2024.","apa":"Van Vliet, S., Sheng, J., Stindt, C. N., &#38; Feringa, B. L. (2024). All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-024-50587-4\">https://doi.org/10.1038/s41467-024-50587-4</a>","chicago":"Van Vliet, Sven, Jinyu Sheng, Charlotte N. Stindt, and Ben L. Feringa. “All-Visible-Light-Driven Salicylidene Schiff-Base-Functionalized Artificial Molecular Motors.” <i>Nature Communications</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41467-024-50587-4\">https://doi.org/10.1038/s41467-024-50587-4</a>.","ista":"Van Vliet S, Sheng J, Stindt CN, Feringa BL. 2024. All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors. Nature Communications. 15, 6461."},"researchdata_availability":"yes","publication":"Nature Communications","volume":15,"year":"2024","intvolume":"        15","quality_controlled":"1","ddc":["540"],"status":"public","scopus_import":"1","pmid":1,"DOAJ_listed":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"text":"Light-driven rotary molecular motors are among the most promising classes of responsive molecular machines and take advantage of their intrinsic chirality which governs unidirectional rotation. As a consequence of their dynamic function, they receive considerable interest in the areas of supramolecular chemistry, asymmetric catalysis and responsive materials. Among the emerging classes of responsive photochromic molecules, multistate first-generation molecular motors driven by benign visible light remain unexplored, which limits the exploitation of the full potential of these mechanical light-powered systems. Herein, we describe a series of all-visible-light-driven first-generation molecular motors based on the salicylidene Schiff base functionality. Remarkable redshifts up to 100 nm in absorption are achieved compared to conventional first-generation motor structures. Taking advantage of all-visible-light-driven multistate motor scaffolds, adaptive behaviour is found as well, and potential application in multistate photoluminescence is demonstrated. These functional visible-light-responsive motors will likely stimulate the design and synthesis of more sophisticated nanomachinery with a myriad of future applications in powering dynamic systems.","lang":"eng"}],"author":[{"last_name":"Van Vliet","first_name":"Sven","full_name":"Van Vliet, Sven"},{"last_name":"Sheng","full_name":"Sheng, Jinyu","first_name":"Jinyu","id":"639f0526-27c9-11ee-95a6-966cd7f102d8"},{"first_name":"Charlotte N.","full_name":"Stindt, Charlotte N.","last_name":"Stindt"},{"last_name":"Feringa","first_name":"Ben L.","full_name":"Feringa, Ben L."}],"oa":1,"file_date_updated":"2024-08-12T08:23:10Z","publisher":"Springer Nature","supplementarymaterial":"yes","month":"07","date_published":"2024-07-31T00:00:00Z","dataavailabilitystatement":"Data that support the findings of this study are available within the Supplementary Information. All data are available from the corresponding author upon request. The XYZ coordinates of the DFT-optimized structures are available as source data. Source data are provided with this paper.","acknowledgement":"Financial support from the Netherlands Organization for Scientific Research (NWO-CW), the Dutch Ministry of Education, Culture and Science (Gravitation program No.024.001.035), the China Scholarship Council (CSC PhD Fellowship No.201808330459 to J. S.). We thank Dr. Youxin Fu, Dr. Alexander Ryabchun, Dr. Wojciech Danowski, Dr. Jianyu Zhang and Yahan Shan from University of Groningen for their help to this project and fruitful discussions.","date_created":"2024-08-11T22:01:11Z","department":[{"_id":"RaKl"}],"date_updated":"2026-09-17T10:46:04Z","das_tickbox":"1","file":[{"file_id":"17418","file_size":1355696,"relation":"main_file","content_type":"application/pdf","file_name":"2024_NatureComm_vanVliet.pdf","success":1,"date_created":"2024-08-12T08:23:10Z","checksum":"4aca2ea30fe529947cb2f4c7fc2af2df","date_updated":"2024-08-12T08:23:10Z","access_level":"open_access","creator":"dernst"}]},{"citation":{"apa":"Sheng, J., Perego, J., Bracco, S., Cieciórski, P., Danowski, W., Comotti, A., &#38; Feringa, B. L. (2024). Orthogonal photoswitching in a porous organic framework. <i>Angewandte Chemie International Edition</i>. Wiley. <a href=\"https://doi.org/10.1002/anie.202404878\">https://doi.org/10.1002/anie.202404878</a>","ama":"Sheng J, Perego J, Bracco S, et al. Orthogonal photoswitching in a porous organic framework. <i>Angewandte Chemie International Edition</i>. 2024;63(23). doi:<a href=\"https://doi.org/10.1002/anie.202404878\">10.1002/anie.202404878</a>","short":"J. Sheng, J. Perego, S. Bracco, P. Cieciórski, W. Danowski, A. Comotti, B.L. Feringa, Angewandte Chemie International Edition 63 (2024).","ieee":"J. Sheng <i>et al.</i>, “Orthogonal photoswitching in a porous organic framework,” <i>Angewandte Chemie International Edition</i>, vol. 63, no. 23. Wiley, 2024.","mla":"Sheng, Jinyu, et al. “Orthogonal Photoswitching in a Porous Organic Framework.” <i>Angewandte Chemie International Edition</i>, vol. 63, no. 23, e202404878, Wiley, 2024, doi:<a href=\"https://doi.org/10.1002/anie.202404878\">10.1002/anie.202404878</a>.","chicago":"Sheng, Jinyu, Jacopo Perego, Silvia Bracco, Piotr Cieciórski, Wojciech Danowski, Angiolina Comotti, and Ben L. Feringa. “Orthogonal Photoswitching in a Porous Organic Framework.” <i>Angewandte Chemie International Edition</i>. Wiley, 2024. <a href=\"https://doi.org/10.1002/anie.202404878\">https://doi.org/10.1002/anie.202404878</a>.","ista":"Sheng J, Perego J, Bracco S, Cieciórski P, Danowski W, Comotti A, Feringa BL. 2024. Orthogonal photoswitching in a porous organic framework. Angewandte Chemie International Edition. 63(23), e202404878."},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"e202404878","publication_identifier":{"eissn":["1521-3773"],"issn":["1433-7851"]},"publication_status":"published","external_id":{"pmid":["38530132"]},"title":"Orthogonal photoswitching in a porous organic framework","issue":"23","article_type":"original","has_accepted_license":"1","type":"journal_article","article_processing_charge":"Yes (in subscription journal)","doi":"10.1002/anie.202404878","fulldoi":"https://doi.org/10.1002/anie.202404878","oa_version":"Published Version","language":[{"iso":"eng"}],"_id":"17105","day":"26","date_updated":"2026-09-17T10:32:51Z","das_tickbox":"1","file":[{"file_size":2363206,"file_id":"17107","content_type":"application/pdf","relation":"main_file","checksum":"7ecb0892051f6ed8f6a6f25baa47543a","file_name":"2024_AngChemieInt_Sheng.pdf","date_created":"2024-06-03T10:33:17Z","success":1,"creator":"dernst","access_level":"open_access","date_updated":"2024-06-03T10:33:17Z"}],"acknowledgement":"This work was supported from the following sources: China Scholarship Council (CSC PhD Fellowship No. 201808330459 to J.S.), the Ministry of Education, Culture and Science of the Netherlands (Gravitation Program No. 024.001.035 to BLF), Financial support from The Netherlands Organization for Scientific Research (NWO-CW), the European Research Council (ERC; advanced Grant No. 694345 to B.L.F.). PRIN (SHERPA 2020 No. H45F21003430001) and PRIN (HySTAR 2022 No. H53D23004720006) and Lombardy Region for “Enhancing Photosynthesis” grant (2021-2023 No. H45F21002830007). W.D. is grateful for financial support from Marie Skłodowska-Curie Actions (Individual Fellowship No. 101027639). P.C. is grateful for the financial support provided by the PRELUDIUM grant from the National Science Center Poland (Reg. No: 2023/49/N/ST5/01864).","date_created":"2024-06-03T09:00:01Z","department":[{"_id":"RaKl"}],"date_published":"2024-03-26T00:00:00Z","dataavailabilitystatement":"The data that support the findings of this study are available in the supplementary material of this article.","supplementarymaterial":"yes","month":"03","publisher":"Wiley","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"The development of photoresponsive systems with non-invasive orthogonal control by distinct wavelengths of light is still in its infancy. In particular, the design of photochemically triggered-orthogonal systems integrated into solid materials that enable multiple dynamic control over their properties remains a longstanding challenge. Here, we report the orthogonal and reversible control of two types of photoswitches in an integrated solid porous framework, that is, visible-light responsive o-fluoroazobenzene and nitro-spiropyran motifs. The properties of the constructed material can be selectively controlled by different wavelengths of light thus generating four distinct states providing a basis for dynamic multifunctional materials. Solid-state NMR spectroscopy demonstrated the selective transformation of the azobenzene switch in the bulk, which in turn modulates N2 and CO2 adsorption."}],"author":[{"last_name":"Sheng","full_name":"Sheng, Jinyu","first_name":"Jinyu","id":"639f0526-27c9-11ee-95a6-966cd7f102d8"},{"full_name":"Perego, Jacopo","first_name":"Jacopo","last_name":"Perego"},{"last_name":"Bracco","first_name":"Silvia","full_name":"Bracco, Silvia"},{"first_name":"Piotr","full_name":"Cieciórski, Piotr","last_name":"Cieciórski"},{"last_name":"Danowski","first_name":"Wojciech","full_name":"Danowski, Wojciech"},{"last_name":"Comotti","first_name":"Angiolina","full_name":"Comotti, Angiolina"},{"first_name":"Ben L.","full_name":"Feringa, Ben L.","last_name":"Feringa"}],"oa":1,"file_date_updated":"2024-06-03T10:33:17Z","intvolume":"        63","status":"public","quality_controlled":"1","ddc":["540"],"pmid":1,"scopus_import":"1","researchdata_availability":"yes","publication":"Angewandte Chemie International Edition","year":"2024","volume":63},{"file_date_updated":"2025-01-09T09:38:51Z","oa":1,"author":[{"last_name":"Shan","first_name":"Yahan","full_name":"Shan, Yahan"},{"last_name":"Sheng","id":"639f0526-27c9-11ee-95a6-966cd7f102d8","first_name":"Jinyu","full_name":"Sheng, Jinyu"},{"first_name":"Qi","full_name":"Zhang, Qi","last_name":"Zhang"},{"last_name":"Stuart","full_name":"Stuart, Marc C.A.","first_name":"Marc C.A."},{"last_name":"Qu","full_name":"Qu, Da Hui","first_name":"Da Hui"},{"full_name":"Feringa, Ben L.","first_name":"Ben L.","last_name":"Feringa"}],"abstract":[{"lang":"eng","text":"Photoisomerization and photoluminescence are two distinct energy dissipation pathways in light-driven molecular motors. The photoisomerization properties of discrete molecular motors have been well established in solution, but their photoluminescent properties have been rarely reported—especially in aggregates. Here, it is shown that an overcrowded alkene-based molecular motor exhibits distinct dynamic properties in solution and aggregate states, for example, gel and solid states. Despite the poor emissive properties of molecular motors in solution, a bright emission is observed in the aggregate states, including in gel and the crystalline solid. The emission wavelength is highly dependent on the nature of the supramolecular packing and order in the aggregates. As a result, the fluorescent color can be readily tuned reversibly via mechanical grinding and vapor fuming, which provides a new platform for developing multi-stimuli functional materials."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"publisher":"Wiley","volume":5,"year":"2024","publication":"Aggregate","researchdata_availability":"no","scopus_import":"1","OA_type":"gold","status":"public","quality_controlled":"1","ddc":["540"],"intvolume":"         5","department":[{"_id":"RaKl"}],"date_created":"2024-05-26T22:00:58Z","acknowledgement":"This work was supported by the National Natural Science Foundation of China (grant nos. 22220102004, 22025503), Shanghai Municipal Science and Technology Major Project (grant no. 2018SHZDZX03), the Innovation Program of Shanghai Municipal Education Commission (2023ZKZD40), the Fundamental Research Funds for the Central Universities, the Program of Introducing Talents of Discipline to Universities (grant no. B16017), Science and Technology Commission of Shanghai Municipality (grant no. 21JC1401700), and the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study (grant no. SN-ZJU-SIAS-006), China Scholarship Council (CSC PhD Fellowship No. 202006745016 to Yahan Shan). The authors gratefully acknowledge financial support from the Dutch Ministry of Education, Culture and Science (gravitation program no. 024.001.035 to Ben L. Feringa). The authors thank Dr. Youxin Fu and Dr. Alexander Ryabchun for the help with fluorescence quantum yield measurement, Cristina Nitu for the help with photoisomerzation quantum yield measurement, Prof. Wesley R. Browne for the help with fluorescence lifetime measurement, and Dr. Jianyu Zhang for fruitful discussion and revising the manuscript.","file":[{"creator":"dernst","date_updated":"2025-01-09T09:38:51Z","access_level":"open_access","date_created":"2025-01-09T09:38:51Z","file_name":"2024_Aggregate_Shan.pdf","success":1,"checksum":"1e79dc81d0edf0b441ef661a1890bbf5","content_type":"application/pdf","relation":"main_file","file_id":"18804","file_size":2299084}],"das_tickbox":"0","date_updated":"2026-09-17T11:01:40Z","month":"10","supplementarymaterial":"yes","OA_place":"publisher","date_published":"2024-10-01T00:00:00Z","type":"journal_article","has_accepted_license":"1","article_type":"original","issue":"5","_id":"17054","day":"01","fulldoi":"https://doi.org/10.1002/agt2.584","oa_version":"Published Version","language":[{"iso":"eng"}],"doi":"10.1002/agt2.584","article_processing_charge":"Yes","citation":{"ista":"Shan Y, Sheng J, Zhang Q, Stuart MCA, Qu DH, Feringa BL. 2024. Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates. Aggregate. 5(5), e584.","chicago":"Shan, Yahan, Jinyu Sheng, Qi Zhang, Marc C.A. Stuart, Da Hui Qu, and Ben L. Feringa. “Multi-State Photoluminescent Properties of an Overcrowded Alkene-Based Molecular Motor in Aggregates.” <i>Aggregate</i>. Wiley, 2024. <a href=\"https://doi.org/10.1002/agt2.584\">https://doi.org/10.1002/agt2.584</a>.","ieee":"Y. Shan, J. Sheng, Q. Zhang, M. C. A. Stuart, D. H. Qu, and B. L. Feringa, “Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates,” <i>Aggregate</i>, vol. 5, no. 5. Wiley, 2024.","mla":"Shan, Yahan, et al. “Multi-State Photoluminescent Properties of an Overcrowded Alkene-Based Molecular Motor in Aggregates.” <i>Aggregate</i>, vol. 5, no. 5, e584, Wiley, 2024, doi:<a href=\"https://doi.org/10.1002/agt2.584\">10.1002/agt2.584</a>.","ama":"Shan Y, Sheng J, Zhang Q, Stuart MCA, Qu DH, Feringa BL. Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates. <i>Aggregate</i>. 2024;5(5). doi:<a href=\"https://doi.org/10.1002/agt2.584\">10.1002/agt2.584</a>","short":"Y. Shan, J. Sheng, Q. Zhang, M.C.A. Stuart, D.H. Qu, B.L. Feringa, Aggregate 5 (2024).","apa":"Shan, Y., Sheng, J., Zhang, Q., Stuart, M. C. A., Qu, D. H., &#38; Feringa, B. L. (2024). Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates. <i>Aggregate</i>. Wiley. <a href=\"https://doi.org/10.1002/agt2.584\">https://doi.org/10.1002/agt2.584</a>"},"title":"Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates","publication_status":"published","publication_identifier":{"issn":["2766-8541"],"eissn":["2692-4560"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"e584"},{"year":"2024","volume":52,"publication":"Alternatives to Laboratory Animals","researchdata_availability":"no","scopus_import":"1","pmid":1,"quality_controlled":"1","OA_type":"closed access","status":"public","intvolume":"        52","author":[{"full_name":"Kitsara, Maria","first_name":"Maria","last_name":"Kitsara"},{"full_name":"Smajlhodžić-Deljo, Merima","first_name":"Merima","last_name":"Smajlhodžić-Deljo"},{"first_name":"Lejla","full_name":"Gurbeta Pokvic, Lejla","last_name":"Gurbeta Pokvic"},{"first_name":"Bettina","full_name":"Bert, Bettina","last_name":"Bert"},{"last_name":"Bubalo","full_name":"Bubalo, Nataliia","first_name":"Nataliia"},{"last_name":"Erden","first_name":"Sevilay","full_name":"Erden, Sevilay"},{"last_name":"Franco","full_name":"Franco, Nuno Henrique","first_name":"Nuno Henrique"},{"full_name":"Chirico, Giuseppe","first_name":"Giuseppe","last_name":"Chirico"},{"full_name":"Gómez Raja, Jonathan","first_name":"Jonathan","last_name":"Gómez Raja"},{"last_name":"Gonzalez-Uarquin","full_name":"Gonzalez-Uarquin, Fernando","first_name":"Fernando"},{"last_name":"Lang","first_name":"Annemarie","full_name":"Lang, Annemarie"},{"first_name":"Nicole","full_name":"Linklater, Nicole","last_name":"Linklater"},{"last_name":"Mojsova","first_name":"Sandra","full_name":"Mojsova, Sandra"},{"last_name":"Olsson","full_name":"Olsson, I. Anna S.","first_name":"I. Anna S."},{"last_name":"Sandvig","full_name":"Sandvig, Ioanna","first_name":"Ioanna"},{"last_name":"Schaffert","first_name":"Alexandra","full_name":"Schaffert, Alexandra"},{"full_name":"Schmit, Marthe","first_name":"Marthe","last_name":"Schmit"},{"last_name":"Schober","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","first_name":"Sophie","full_name":"Schober, Sophie"},{"first_name":"Bogdan","full_name":"Sevastre, Bogdan","last_name":"Sevastre"},{"last_name":"Wilflingseder","first_name":"Doris","full_name":"Wilflingseder, Doris"},{"last_name":"Ahluwalia","full_name":"Ahluwalia, Arti","first_name":"Arti"},{"first_name":"Winfried","full_name":"Neuhaus, Winfried","last_name":"Neuhaus"}],"publisher":"SAGE Publications","month":"11","supplementarymaterial":"no","date_published":"2024-11-01T00:00:00Z","department":[{"_id":"PreCl"}],"date_created":"2024-10-13T22:01:51Z","das_tickbox":"0","date_updated":"2026-09-17T10:30:08Z","_id":"18310","day":"01","language":[{"iso":"eng"}],"fulldoi":"https://doi.org/10.1177/02611929241286024","oa_version":"None","doi":"10.1177/02611929241286024","article_processing_charge":"No","type":"journal_article","article_type":"original","page":"326-333","isi":1,"issue":"6","title":"Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE)","external_id":{"isi":["001348633700007"],"pmid":["39333027"]},"publication_status":"published","publication_identifier":{"issn":["0261-1929"],"eissn":["2632-3559"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"chicago":"Kitsara, Maria, Merima Smajlhodžić-Deljo, Lejla Gurbeta Pokvic, Bettina Bert, Nataliia Bubalo, Sevilay Erden, Nuno Henrique Franco, et al. “Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE).” <i>Alternatives to Laboratory Animals</i>. SAGE Publications, 2024. <a href=\"https://doi.org/10.1177/02611929241286024\">https://doi.org/10.1177/02611929241286024</a>.","ista":"Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, Bert B, Bubalo N, Erden S, Franco NH, Chirico G, Gómez Raja J, Gonzalez-Uarquin F, Lang A, Linklater N, Mojsova S, Olsson IAS, Sandvig I, Schaffert A, Schmit M, Schober S, Sevastre B, Wilflingseder D, Ahluwalia A, Neuhaus W. 2024. Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). Alternatives to Laboratory Animals. 52(6), 326–333.","ama":"Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, et al. Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). <i>Alternatives to Laboratory Animals</i>. 2024;52(6):326-333. doi:<a href=\"https://doi.org/10.1177/02611929241286024\">10.1177/02611929241286024</a>","short":"M. Kitsara, M. Smajlhodžić-Deljo, L. Gurbeta Pokvic, B. Bert, N. Bubalo, S. Erden, N.H. Franco, G. Chirico, J. Gómez Raja, F. Gonzalez-Uarquin, A. Lang, N. Linklater, S. Mojsova, I.A.S. Olsson, I. Sandvig, A. Schaffert, M. Schmit, S. Schober, B. Sevastre, D. Wilflingseder, A. Ahluwalia, W. Neuhaus, Alternatives to Laboratory Animals 52 (2024) 326–333.","ieee":"M. Kitsara <i>et al.</i>, “Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE),” <i>Alternatives to Laboratory Animals</i>, vol. 52, no. 6. SAGE Publications, pp. 326–333, 2024.","mla":"Kitsara, Maria, et al. “Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE).” <i>Alternatives to Laboratory Animals</i>, vol. 52, no. 6, SAGE Publications, 2024, pp. 326–33, doi:<a href=\"https://doi.org/10.1177/02611929241286024\">10.1177/02611929241286024</a>.","apa":"Kitsara, M., Smajlhodžić-Deljo, M., Gurbeta Pokvic, L., Bert, B., Bubalo, N., Erden, S., … Neuhaus, W. (2024). Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). <i>Alternatives to Laboratory Animals</i>. SAGE Publications. <a href=\"https://doi.org/10.1177/02611929241286024\">https://doi.org/10.1177/02611929241286024</a>"}},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"48","title":"A note on the binding energy for Bosons in the mean-field limit","publication_identifier":{"issn":["0022-4715"],"eissn":["1572-9613"]},"publication_status":"published","external_id":{"isi":["001197663100002"],"arxiv":["2307.13115"]},"citation":{"ista":"Bossmann L, Leopold NK, Mitrouskas DJ, Petrat SP. 2024. A note on the binding energy for Bosons in the mean-field limit. Journal of Statistical Physics. 191(4), 48.","chicago":"Bossmann, Lea, Nikolai K Leopold, David Johannes Mitrouskas, and Sören P Petrat. “A Note on the Binding Energy for Bosons in the Mean-Field Limit.” <i>Journal of Statistical Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1007/s10955-024-03260-5\">https://doi.org/10.1007/s10955-024-03260-5</a>.","apa":"Bossmann, L., Leopold, N. K., Mitrouskas, D. J., &#38; Petrat, S. P. (2024). A note on the binding energy for Bosons in the mean-field limit. <i>Journal of Statistical Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1007/s10955-024-03260-5\">https://doi.org/10.1007/s10955-024-03260-5</a>","mla":"Bossmann, Lea, et al. “A Note on the Binding Energy for Bosons in the Mean-Field Limit.” <i>Journal of Statistical Physics</i>, vol. 191, no. 4, 48, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1007/s10955-024-03260-5\">10.1007/s10955-024-03260-5</a>.","ieee":"L. Bossmann, N. K. Leopold, D. J. Mitrouskas, and S. P. Petrat, “A note on the binding energy for Bosons in the mean-field limit,” <i>Journal of Statistical Physics</i>, vol. 191, no. 4. Springer Nature, 2024.","short":"L. Bossmann, N.K. Leopold, D.J. Mitrouskas, S.P. Petrat, Journal of Statistical Physics 191 (2024).","ama":"Bossmann L, Leopold NK, Mitrouskas DJ, Petrat SP. A note on the binding energy for Bosons in the mean-field limit. <i>Journal of Statistical Physics</i>. 2024;191(4). doi:<a href=\"https://doi.org/10.1007/s10955-024-03260-5\">10.1007/s10955-024-03260-5</a>"},"article_processing_charge":"Yes (via OA deal)","fulldoi":"https://doi.org/10.1007/s10955-024-03260-5","language":[{"iso":"eng"}],"oa_version":"Published Version","day":"06","_id":"15318","doi":"10.1007/s10955-024-03260-5","article_type":"original","issue":"4","arxiv":1,"isi":1,"has_accepted_license":"1","type":"journal_article","dataavailabilitystatement":"Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.","date_published":"2024-04-06T00:00:00Z","month":"04","supplementarymaterial":"no","file":[{"file_size":398665,"file_id":"15325","relation":"main_file","content_type":"application/pdf","checksum":"839242a9ec1c01158112de25f196e60d","file_name":"2024_JourStatPhysics_Bossmann.pdf","success":1,"date_created":"2024-04-16T11:09:37Z","creator":"dernst","access_level":"open_access","date_updated":"2024-04-16T11:09:37Z"}],"date_updated":"2026-09-17T11:05:18Z","das_tickbox":"1","department":[{"_id":"RoSe"}],"acknowledgement":"It is a pleasure to thank Phan Thành Nam for helpful discussions on bosonic atoms. L.B. was supported by the German Research Foundation within the Munich Center of Quantum Science and Technology (EXC 2111). N.L. gratefully acknowledges support from the Swiss National Science Foundation through the NCCR SwissMap and funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant agreement No 101024712. S.P. acknowledges funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—Project number 512258249.\r\nOpen Access funding enabled and organized by Projekt DEAL.","date_created":"2024-04-14T22:01:02Z","ddc":["510"],"status":"public","quality_controlled":"1","scopus_import":"1","intvolume":"       191","publication":"Journal of Statistical Physics","year":"2024","volume":191,"researchdata_availability":"not applicable","publisher":"Springer Nature","author":[{"last_name":"Bossmann","full_name":"Bossmann, Lea","id":"A2E3BCBE-5FCC-11E9-AA4B-76F3E5697425","first_name":"Lea","orcid":"0000-0002-6854-1343"},{"last_name":"Leopold","orcid":"0000-0002-0495-6822","id":"4BC40BEC-F248-11E8-B48F-1D18A9856A87","first_name":"Nikolai K","full_name":"Leopold, Nikolai K"},{"full_name":"Mitrouskas, David Johannes","id":"cbddacee-2b11-11eb-a02e-a2e14d04e52d","first_name":"David Johannes","last_name":"Mitrouskas"},{"last_name":"Petrat","id":"40AC02DC-F248-11E8-B48F-1D18A9856A87","first_name":"Sören P","orcid":"0000-0002-9166-5889","full_name":"Petrat, Sören P"}],"oa":1,"file_date_updated":"2024-04-16T11:09:37Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"We consider a gas of N weakly interacting bosons in the ground state. Such gases exhibit Bose–Einstein condensation. The binding energy is defined as the energy it takes to remove one particle from the gas. In this article, we prove an asymptotic expansion for the binding energy, and compute the first orders explicitly for the homogeneous gas. Our result addresses in particular a conjecture by Nam (Lett Math Phys 108(1):141–159, 2018), and provides an asymptotic expansion of the ionization energy of bosonic atoms."}]}]