[{"scopus_import":"1","ec_funded":1,"citation":{"apa":"Igler, C., Lagator, M., Tkačik, G., Bollback, J. P., &#38; Guet, C. C. (2018). Evolutionary potential of transcription factors for gene regulatory rewiring. <i>Nature Ecology and Evolution</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41559-018-0651-y\">https://doi.org/10.1038/s41559-018-0651-y</a>","ista":"Igler C, Lagator M, Tkačik G, Bollback JP, Guet CC. 2018. Evolutionary potential of transcription factors for gene regulatory rewiring. Nature Ecology and Evolution. 2(10), 1633–1643.","short":"C. Igler, M. Lagator, G. Tkačik, J.P. Bollback, C.C. Guet, Nature Ecology and Evolution 2 (2018) 1633–1643.","mla":"Igler, Claudia, et al. “Evolutionary Potential of Transcription Factors for Gene Regulatory Rewiring.” <i>Nature Ecology and Evolution</i>, vol. 2, no. 10, Nature Publishing Group, 2018, pp. 1633–43, doi:<a href=\"https://doi.org/10.1038/s41559-018-0651-y\">10.1038/s41559-018-0651-y</a>.","chicago":"Igler, Claudia, Mato Lagator, Gašper Tkačik, Jonathan P Bollback, and Calin C Guet. “Evolutionary Potential of Transcription Factors for Gene Regulatory Rewiring.” <i>Nature Ecology and Evolution</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41559-018-0651-y\">https://doi.org/10.1038/s41559-018-0651-y</a>.","ama":"Igler C, Lagator M, Tkačik G, Bollback JP, Guet CC. Evolutionary potential of transcription factors for gene regulatory rewiring. <i>Nature Ecology and Evolution</i>. 2018;2(10):1633-1643. doi:<a href=\"https://doi.org/10.1038/s41559-018-0651-y\">10.1038/s41559-018-0651-y</a>","ieee":"C. Igler, M. Lagator, G. Tkačik, J. P. Bollback, and C. C. Guet, “Evolutionary potential of transcription factors for gene regulatory rewiring,” <i>Nature Ecology and Evolution</i>, vol. 2, no. 10. Nature Publishing Group, pp. 1633–1643, 2018."},"file_date_updated":"2020-07-14T12:47:37Z","has_accepted_license":"1","date_published":"2018-09-10T00:00:00Z","external_id":{"isi":["000447947600021"]},"doi":"10.1038/s41559-018-0651-y","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","day":"10","article_type":"original","issue":"10","status":"public","month":"09","ddc":["570"],"isi":1,"author":[{"last_name":"Igler","first_name":"Claudia","full_name":"Igler, Claudia","orcid":"0000-0001-7777-546X","id":"46613666-F248-11E8-B48F-1D18A9856A87"},{"id":"345D25EC-F248-11E8-B48F-1D18A9856A87","full_name":"Lagator, Mato","first_name":"Mato","last_name":"Lagator"},{"id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","full_name":"Tkacik, Gasper","orcid":"0000-0002-6699-1455","first_name":"Gasper","last_name":"Tkacik"},{"last_name":"Bollback","first_name":"Jonathan P","orcid":"0000-0002-4624-4612","full_name":"Bollback, Jonathan P","id":"2C6FA9CC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052","last_name":"Guet"}],"quality_controlled":"1","related_material":{"record":[{"id":"5585","status":"public","relation":"popular_science"},{"relation":"dissertation_contains","status":"public","id":"6371"}]},"publication":"Nature Ecology and Evolution","file":[{"date_updated":"2020-07-14T12:47:37Z","relation":"main_file","access_level":"open_access","file_name":"2018_NatureEcology_Igler.pdf","file_id":"7830","checksum":"383a2e2c944a856e2e821ec8e7bf71b6","content_type":"application/pdf","creator":"dernst","date_created":"2020-05-14T11:28:52Z","file_size":1135973}],"date_updated":"2026-10-05T22:30:39Z","intvolume":"         2","volume":2,"page":"1633 - 1643","abstract":[{"lang":"eng","text":"Gene regulatory networks evolve through rewiring of individual components—that is, through changes in regulatory connections. However, the mechanistic basis of regulatory rewiring is poorly understood. Using a canonical gene regulatory system, we quantify the properties of transcription factors that determine the evolutionary potential for rewiring of regulatory connections: robustness, tunability and evolvability. In vivo repression measurements of two repressors at mutated operator sites reveal their contrasting evolutionary potential: while robustness and evolvability were positively correlated, both were in trade-off with tunability. Epistatic interactions between adjacent operators alleviated this trade-off. A thermodynamic model explains how the differences in robustness, tunability and evolvability arise from biophysical characteristics of repressor–DNA binding. The model also uncovers that the energy matrix, which describes how mutations affect repressor–DNA binding, encodes crucial information about the evolutionary potential of a repressor. The biophysical determinants of evolutionary potential for regulatory rewiring constitute a mechanistic framework for understanding network evolution."}],"project":[{"grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"},{"grant_number":"648440","name":"Selective Barriers to Horizontal Gene Transfer","_id":"2578D616-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"24573","_id":"251EE76E-B435-11E9-9278-68D0E5697425","name":"Design principles underlying genetic switch architecture"}],"year":"2018","language":[{"iso":"eng"}],"publisher":"Nature Publishing Group","type":"journal_article","publist_id":"7987","oa":1,"article_processing_charge":"No","publication_status":"published","oa_version":"Submitted Version","fulldoi":"https://doi.org/10.1038/s41559-018-0651-y","date_created":"2018-12-11T11:44:27Z","title":"Evolutionary potential of transcription factors for gene regulatory rewiring","_id":"67","department":[{"_id":"CaGu"},{"_id":"GaTk"},{"_id":"JoBo"}]},{"type":"research_data","publisher":"Institute of Science and Technology Austria","project":[{"grant_number":"291734","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"call_identifier":"H2020","name":"Selective Barriers to Horizontal Gene Transfer","_id":"2578D616-B435-11E9-9278-68D0E5697425","grant_number":"648440"},{"grant_number":"24573","_id":"251EE76E-B435-11E9-9278-68D0E5697425","name":"Design principles underlying genetic switch architecture"}],"year":"2018","abstract":[{"text":"Mean repression values and standard error of the mean are given for all operator mutant libraries.","lang":"eng"}],"date_updated":"2026-10-05T22:30:39Z","file":[{"access_level":"open_access","file_name":"IST-2018-108-v1+1_data_figures.xlsx","date_updated":"2020-07-14T12:47:07Z","relation":"main_file","creator":"system","checksum":"1435781526c77413802adee0d4583cce","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","file_id":"5611","file_size":16507,"date_created":"2018-12-12T13:02:45Z"}],"department":[{"_id":"CaGu"},{"_id":"GaTk"}],"_id":"5585","title":"Data for the paper Evolutionary potential of transcription factors for gene regulatory rewiring","date_created":"2018-12-12T12:31:40Z","fulldoi":"https://doi.org/10.15479/AT:ISTA:108","oa_version":"Published Version","article_processing_charge":"No","oa":1,"tmp":{"name":"Creative Commons Public Domain Dedication (CC0 1.0)","legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","short":"CC0 (1.0)","image":"/images/cc_0.png"},"date_published":"2018-07-20T00:00:00Z","has_accepted_license":"1","file_date_updated":"2020-07-14T12:47:07Z","citation":{"ieee":"C. Igler, M. Lagator, G. Tkačik, J. P. Bollback, and C. C. Guet, “Data for the paper Evolutionary potential of transcription factors for gene regulatory rewiring.” Institute of Science and Technology Austria, 2018.","ama":"Igler C, Lagator M, Tkačik G, Bollback JP, Guet CC. Data for the paper Evolutionary potential of transcription factors for gene regulatory rewiring. 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:108\">10.15479/AT:ISTA:108</a>","chicago":"Igler, Claudia, Mato Lagator, Gašper Tkačik, Jonathan P Bollback, and Calin C Guet. “Data for the Paper Evolutionary Potential of Transcription Factors for Gene Regulatory Rewiring.” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:108\">https://doi.org/10.15479/AT:ISTA:108</a>.","ista":"Igler C, Lagator M, Tkačik G, Bollback JP, Guet CC. 2018. Data for the paper Evolutionary potential of transcription factors for gene regulatory rewiring, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:108\">10.15479/AT:ISTA:108</a>.","short":"C. Igler, M. Lagator, G. Tkačik, J.P. Bollback, C.C. Guet, (2018).","apa":"Igler, C., Lagator, M., Tkačik, G., Bollback, J. P., &#38; Guet, C. C. (2018). Data for the paper Evolutionary potential of transcription factors for gene regulatory rewiring. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:108\">https://doi.org/10.15479/AT:ISTA:108</a>","mla":"Igler, Claudia, et al. <i>Data for the Paper Evolutionary Potential of Transcription Factors for Gene Regulatory Rewiring</i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:108\">10.15479/AT:ISTA:108</a>."},"ec_funded":1,"datarep_id":"108","related_material":{"record":[{"id":"67","status":"public","relation":"research_paper"},{"id":"6371","status":"public","relation":"research_paper"}]},"author":[{"first_name":"Claudia","full_name":"Igler, Claudia","orcid":"0000-0001-7777-546X","id":"46613666-F248-11E8-B48F-1D18A9856A87","last_name":"Igler"},{"last_name":"Lagator","first_name":"Mato","full_name":"Lagator, Mato","id":"345D25EC-F248-11E8-B48F-1D18A9856A87"},{"id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","full_name":"Tkacik, Gasper","orcid":"0000-0002-6699-1455","first_name":"Gasper","last_name":"Tkacik"},{"last_name":"Bollback","orcid":"0000-0002-4624-4612","full_name":"Bollback, Jonathan P","id":"2C6FA9CC-F248-11E8-B48F-1D18A9856A87","first_name":"Jonathan P"},{"id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052","first_name":"Calin C","last_name":"Guet"}],"month":"07","ddc":["576"],"status":"public","license":"https://creativecommons.org/publicdomain/zero/1.0/","day":"20","doi":"10.15479/AT:ISTA:108","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"publication":"Nature Communications","quality_controlled":"1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"6363"}],"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/lateral-inhibition-keeps-similar-memories-apart/"}]},"ddc":["570"],"status":"public","month":"11","isi":1,"author":[{"orcid":"0000-0003-4710-2082","full_name":"Espinoza Martinez, Claudia ","id":"31FFEE2E-F248-11E8-B48F-1D18A9856A87","first_name":"Claudia ","last_name":"Espinoza Martinez"},{"full_name":"Guzmán, José","orcid":"0000-0003-2209-5242","id":"30CC5506-F248-11E8-B48F-1D18A9856A87","first_name":"José","last_name":"Guzmán"},{"orcid":"0000-0003-0256-6529","full_name":"Zhang, Xiaomin","id":"423EC9C2-F248-11E8-B48F-1D18A9856A87","first_name":"Xiaomin","last_name":"Zhang"},{"full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M","last_name":"Jonas"}],"issue":"1","day":"02","article_type":"original","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","doi":"10.1038/s41467-018-06899-3","external_id":{"isi":["000449069700009"]},"date_published":"2018-11-02T00:00:00Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"file_date_updated":"2020-07-14T12:45:28Z","acknowledgement":"This project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 692692) and the Fond zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award), both to P.J..","has_accepted_license":"1","citation":{"ama":"Espinoza Martinez C, Guzmán J, Zhang X, Jonas PM. Parvalbumin+ interneurons obey unique connectivity rules and establish a powerful lateral-inhibition microcircuit in dentate gyrus. <i>Nature Communications</i>. 2018;9(1). doi:<a href=\"https://doi.org/10.1038/s41467-018-06899-3\">10.1038/s41467-018-06899-3</a>","ieee":"C. Espinoza Martinez, J. Guzmán, X. Zhang, and P. M. Jonas, “Parvalbumin+ interneurons obey unique connectivity rules and establish a powerful lateral-inhibition microcircuit in dentate gyrus,” <i>Nature Communications</i>, vol. 9, no. 1. Nature Publishing Group, 2018.","chicago":"Espinoza Martinez, Claudia , José Guzmán, Xiaomin Zhang, and Peter M Jonas. “Parvalbumin+ Interneurons Obey Unique Connectivity Rules and Establish a Powerful Lateral-Inhibition Microcircuit in Dentate Gyrus.” <i>Nature Communications</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41467-018-06899-3\">https://doi.org/10.1038/s41467-018-06899-3</a>.","apa":"Espinoza Martinez, C., Guzmán, J., Zhang, X., &#38; Jonas, P. M. (2018). Parvalbumin+ interneurons obey unique connectivity rules and establish a powerful lateral-inhibition microcircuit in dentate gyrus. <i>Nature Communications</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41467-018-06899-3\">https://doi.org/10.1038/s41467-018-06899-3</a>","ista":"Espinoza Martinez C, Guzmán J, Zhang X, Jonas PM. 2018. Parvalbumin+ interneurons obey unique connectivity rules and establish a powerful lateral-inhibition microcircuit in dentate gyrus. Nature Communications. 9(1), 4605.","short":"C. Espinoza Martinez, J. Guzmán, X. Zhang, P.M. Jonas, Nature Communications 9 (2018).","mla":"Espinoza Martinez, Claudia, et al. “Parvalbumin+ Interneurons Obey Unique Connectivity Rules and Establish a Powerful Lateral-Inhibition Microcircuit in Dentate Gyrus.” <i>Nature Communications</i>, vol. 9, no. 1, 4605, Nature Publishing Group, 2018, doi:<a href=\"https://doi.org/10.1038/s41467-018-06899-3\">10.1038/s41467-018-06899-3</a>."},"ec_funded":1,"scopus_import":"1","department":[{"_id":"PeJo"}],"title":"Parvalbumin+ interneurons obey unique connectivity rules and establish a powerful lateral-inhibition microcircuit in dentate gyrus","_id":"21","date_created":"2018-12-11T11:44:12Z","oa_version":"Published Version","fulldoi":"https://doi.org/10.1038/s41467-018-06899-3","publication_status":"published","article_processing_charge":"No","oa":1,"publist_id":"8034","publisher":"Nature Publishing Group","type":"journal_article","project":[{"grant_number":"692692","call_identifier":"H2020","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"},{"grant_number":"Z00312","call_identifier":"FWF","name":"Synaptic communication in neuronal microcircuits","_id":"25C5A090-B435-11E9-9278-68D0E5697425"}],"year":"2018","language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"Parvalbumin-positive (PV+) GABAergic interneurons in hippocampal microcircuits are thought to play a key role in several higher network functions, such as feedforward and feedback inhibition, network oscillations, and pattern separation. Fast lateral inhibition mediated by GABAergic interneurons may implement a winner-takes-all mechanism in the hippocampal input layer. However, it is not clear whether the functional connectivity rules of granule cells (GCs) and interneurons in the dentate gyrus are consistent with such a mechanism. Using simultaneous patch-clamp recordings from up to seven GCs and up to four PV+ interneurons in the dentate gyrus, we find that connectivity is structured in space, synapse-specific, and enriched in specific disynaptic motifs. In contrast to the neocortex, lateral inhibition in the dentate gyrus (in which a GC inhibits neighboring GCs via a PV+ interneuron) is ~ 10-times more abundant than recurrent inhibition (in which a GC inhibits itself). Thus, unique connectivity rules may enable the dentate gyrus to perform specific higher-order computations"}],"volume":9,"article_number":"4605","intvolume":"         9","date_updated":"2026-10-05T22:30:41Z","file":[{"file_size":4651930,"date_created":"2018-12-17T15:41:57Z","relation":"main_file","date_updated":"2020-07-14T12:45:28Z","access_level":"open_access","file_name":"2018_NatureComm_Espinoza.pdf","creator":"dernst","file_id":"5715","checksum":"9fe2a63bd95a5067d896c087d07998f3","content_type":"application/pdf"}]},{"date_created":"2018-12-11T11:44:21Z","publication_identifier":{"issn":["2663-337X"]},"_id":"50","title":"Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration","degree_awarded":"PhD","department":[{"_id":"CaHe"}],"oa":1,"article_processing_charge":"No","OA_place":"publisher","publication_status":"published","fulldoi":"https://doi.org/10.15479/AT:ISTA:TH_1031","oa_version":"Published Version","language":[{"iso":"eng"}],"year":"2018","type":"dissertation","publisher":"Institute of Science and Technology Austria","publist_id":"8004","file":[{"file_id":"6238","content_type":"application/pdf","checksum":"d3eca3dcacb67bffdde6e6609c31cdd0","creator":"dernst","relation":"main_file","date_updated":"2021-02-11T11:17:17Z","access_level":"open_access","file_name":"2018_Thesis_Capek.pdf","embargo":"2019-06-25","date_created":"2019-04-08T13:42:26Z","file_size":31576521},{"file_size":38992956,"date_created":"2019-04-08T13:42:27Z","creator":"dernst","embargo_to":"open_access","file_id":"6239","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"876deb14067e638aba65d209668bd821","date_updated":"2021-02-11T23:30:21Z","relation":"source_file","file_name":"2018_Thesis_Capek_source.docx","access_level":"closed"}],"date_updated":"2026-04-16T10:07:33Z","page":"95","pubrep_id":"1031","abstract":[{"text":"The Wnt/planar cell polarity (Wnt/PCP) pathway determines planar polarity of epithelial cells in both vertebrates and invertebrates. The role that Wnt/PCP signaling plays in mesenchymal contexts, however, is only poorly understood. While previous studies have demonstrated the capacity of Wnt/PCP signaling to polarize and guide directed migration of mesenchymal cells, it remains unclear whether endogenous Wnt/PCP signaling performs these functions instructively, as it does in epithelial cells. Here we developed a light-switchable version of the Wnt/PCP receptor Frizzled 7 (Fz7) to unambiguously distinguish between an instructive and a permissive role of Wnt/PCP signaling for the directional collective migration of mesendoderm progenitor cells during zebrafish gastrulation. We show that prechordal plate (ppl) cell migration is defective in maternal-zygotic fz7a and fz7b (MZ fz7a,b) double mutant embryos, and that Fz7 functions cell-autonomously in this process by promoting ppl cell protrusion formation and directed migration. We further show that local activation of Fz7 can direct ppl cell migration both in vitro and in vivo. Surprisingly, however, uniform Fz7 activation is sufficient to fully rescue the ppl cell migration defect in MZ fz7a,b mutant embryos, indicating that Wnt/PCP signaling functions permissively rather than instructively in directed mesendoderm cell migration during zebrafish gastrulation.","lang":"eng"}],"author":[{"first_name":"Daniel","id":"31C42484-F248-11E8-B48F-1D18A9856A87","full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940","last_name":"Capek"}],"status":"public","month":"06","ddc":["570","591","596"],"related_material":{"record":[{"id":"661","status":"public","relation":"part_of_dissertation"},{"status":"public","relation":"part_of_dissertation","id":"1100"},{"relation":"part_of_dissertation","status":"public","id":"676"}]},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","doi":"10.15479/AT:ISTA:TH_1031","alternative_title":["ISTA Thesis"],"supervisor":[{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"day":"22","file_date_updated":"2021-02-11T23:30:21Z","has_accepted_license":"1","date_published":"2018-06-22T00:00:00Z","corr_author":"1","citation":{"ista":"Capek D. 2018. Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration. Institute of Science and Technology Austria.","apa":"Capek, D. (2018). <i>Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:TH_1031\">https://doi.org/10.15479/AT:ISTA:TH_1031</a>","short":"D. Capek, Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP Signaling in Directed Mesenchymal Cell Migration, Institute of Science and Technology Austria, 2018.","mla":"Capek, Daniel. <i>Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP Signaling in Directed Mesenchymal Cell Migration</i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:TH_1031\">10.15479/AT:ISTA:TH_1031</a>.","chicago":"Capek, Daniel. “Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP Signaling in Directed Mesenchymal Cell Migration.” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:TH_1031\">https://doi.org/10.15479/AT:ISTA:TH_1031</a>.","ama":"Capek D. Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration. 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:TH_1031\">10.15479/AT:ISTA:TH_1031</a>","ieee":"D. Capek, “Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration,” Institute of Science and Technology Austria, 2018."}},{"publisher":"AIP Publishing","type":"journal_article","year":"2018","language":[{"iso":"eng"}],"intvolume":"        89","date_updated":"2026-10-05T22:30:43Z","abstract":[{"lang":"eng","text":"Solid-state qubit manipulation and read-out fidelities are reaching fault-tolerance, but quantum error correction requires millions of physical qubits and therefore a scalable quantum computer architecture. To solve signal-line bandwidth and fan-out problems, microwave sources required for qubit manipulation might be embedded close to the qubit chip, typically operating at temperatures below 4 K. Here, we perform the first low temperature measurements of a 130 nm BiCMOS based SiGe voltage controlled oscillator at cryogenic temperature. We determined the frequency and output power dependence on temperature and magnetic field up to 5 T and measured the temperature influence on its noise performance. The device maintains its full functionality from 300 K to 4 K. The carrier frequency at 4 K increases by 3% with respect to the carrier frequency at 300 K, and the output power at 4 K increases by 10 dB relative to the output power at 300 K. The frequency tuning range of approximately 20% remains unchanged between 300 K and 4 K. In an in-plane magnetic field of 5 T, the carrier frequency shifts by only 0.02% compared to the frequency at zero magnetic field."}],"arxiv":1,"volume":89,"article_number":"114701","publication_identifier":{"issn":["0034-6748"]},"date_created":"2019-01-10T14:22:23Z","department":[{"_id":"GeKa"}],"title":"30 GHz-voltage controlled oscillator operating at 4 K","_id":"5816","article_processing_charge":"No","oa":1,"oa_version":"Preprint","fulldoi":"https://doi.org/10.1063/1.5038258","publication_status":"published","date_published":"2018-11-01T00:00:00Z","external_id":{"arxiv":["1804.09522"],"isi":["000451735700054"]},"scopus_import":"1","citation":{"ieee":"A. Hollmann, D. Jirovec, M. Kucharski, D. Kissinger, G. Fischer, and L. R. Schreiber, “30 GHz-voltage controlled oscillator operating at 4 K,” <i>Review of Scientific Instruments</i>, vol. 89, no. 11. AIP Publishing, 2018.","ama":"Hollmann A, Jirovec D, Kucharski M, Kissinger D, Fischer G, Schreiber LR. 30 GHz-voltage controlled oscillator operating at 4 K. <i>Review of Scientific Instruments</i>. 2018;89(11). doi:<a href=\"https://doi.org/10.1063/1.5038258\">10.1063/1.5038258</a>","mla":"Hollmann, Arne, et al. “30 GHz-Voltage Controlled Oscillator Operating at 4 K.” <i>Review of Scientific Instruments</i>, vol. 89, no. 11, 114701, AIP Publishing, 2018, doi:<a href=\"https://doi.org/10.1063/1.5038258\">10.1063/1.5038258</a>.","apa":"Hollmann, A., Jirovec, D., Kucharski, M., Kissinger, D., Fischer, G., &#38; Schreiber, L. R. (2018). 30 GHz-voltage controlled oscillator operating at 4 K. <i>Review of Scientific Instruments</i>. AIP Publishing. <a href=\"https://doi.org/10.1063/1.5038258\">https://doi.org/10.1063/1.5038258</a>","short":"A. Hollmann, D. Jirovec, M. Kucharski, D. Kissinger, G. Fischer, L.R. Schreiber, Review of Scientific Instruments 89 (2018).","ista":"Hollmann A, Jirovec D, Kucharski M, Kissinger D, Fischer G, Schreiber LR. 2018. 30 GHz-voltage controlled oscillator operating at 4 K. Review of Scientific Instruments. 89(11), 114701.","chicago":"Hollmann, Arne, Daniel Jirovec, Maciej Kucharski, Dietmar Kissinger, Gunter Fischer, and Lars R. Schreiber. “30 GHz-Voltage Controlled Oscillator Operating at 4 K.” <i>Review of Scientific Instruments</i>. AIP Publishing, 2018. <a href=\"https://doi.org/10.1063/1.5038258\">https://doi.org/10.1063/1.5038258</a>."},"status":"public","isi":1,"month":"11","author":[{"last_name":"Hollmann","full_name":"Hollmann, Arne","first_name":"Arne"},{"first_name":"Daniel","id":"4C473F58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7197-4801","full_name":"Jirovec, Daniel","last_name":"Jirovec"},{"last_name":"Kucharski","first_name":"Maciej","full_name":"Kucharski, Maciej"},{"full_name":"Kissinger, Dietmar","first_name":"Dietmar","last_name":"Kissinger"},{"first_name":"Gunter","full_name":"Fischer, Gunter","last_name":"Fischer"},{"last_name":"Schreiber","full_name":"Schreiber, Lars R.","first_name":"Lars R."}],"issue":"11","main_file_link":[{"url":"https://arxiv.org/abs/1804.09522","open_access":"1"}],"publication":"Review of Scientific Instruments","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"10058"}]},"quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1063/1.5038258","day":"01"},{"article_type":"original","day":"05","doi":"10.21769/BioProtoc.2685","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Bio-protocol","quality_controlled":"1","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"10083"}]},"author":[{"first_name":"Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","full_name":"Li, Lanxin","orcid":"0000-0002-5607-272X","last_name":"Li"},{"first_name":"Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996","last_name":"Krens"},{"first_name":"Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87","full_name":"Fendrych, Matyas","orcid":"0000-0002-9767-8699","last_name":"Fendrych"},{"last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí"}],"ddc":["576","581"],"status":"public","month":"01","issue":"1","citation":{"ista":"Li L, Krens G, Fendrych M, Friml J. 2018. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol. 8(1).","apa":"Li, L., Krens, G., Fendrych, M., &#38; Friml, J. (2018). Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. <i>Bio-Protocol</i>. Bio-protocol. <a href=\"https://doi.org/10.21769/BioProtoc.2685\">https://doi.org/10.21769/BioProtoc.2685</a>","short":"L. Li, G. Krens, M. Fendrych, J. Friml, Bio-Protocol 8 (2018).","mla":"Li, Lanxin, et al. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” <i>Bio-Protocol</i>, vol. 8, no. 1, Bio-protocol, 2018, doi:<a href=\"https://doi.org/10.21769/BioProtoc.2685\">10.21769/BioProtoc.2685</a>.","chicago":"Li, Lanxin, Gabriel Krens, Matyas Fendrych, and Jiří Friml. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” <i>Bio-Protocol</i>. Bio-protocol, 2018. <a href=\"https://doi.org/10.21769/BioProtoc.2685\">https://doi.org/10.21769/BioProtoc.2685</a>.","ieee":"L. Li, G. Krens, M. Fendrych, and J. Friml, “Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls,” <i>Bio-protocol</i>, vol. 8, no. 1. Bio-protocol, 2018.","ama":"Li L, Krens G, Fendrych M, Friml J. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. <i>Bio-protocol</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.21769/BioProtoc.2685\">10.21769/BioProtoc.2685</a>"},"ec_funded":1,"corr_author":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_published":"2018-01-05T00:00:00Z","file_date_updated":"2020-07-14T12:46:29Z","acknowledgement":"This protocol was adapted from Fendrych et al., 2016. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385, and Austrian Science Fund (FWF) [M 2128-B21]. ","has_accepted_license":"1","fulldoi":"https://doi.org/10.21769/BioProtoc.2685","oa_version":"Published Version","publication_status":"published","article_processing_charge":"No","oa":1,"department":[{"_id":"JiFr"},{"_id":"Bio"}],"_id":"442","title":"Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls","publication_identifier":{"eissn":["2331-8325"]},"date_created":"2018-12-11T11:46:30Z","abstract":[{"lang":"eng","text":"The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH."}],"pubrep_id":"970","volume":8,"date_updated":"2026-10-05T22:30:44Z","intvolume":"         8","file":[{"file_size":11352389,"date_created":"2018-12-12T10:17:43Z","creator":"system","checksum":"6644ba698206eda32b0abf09128e63e3","content_type":"application/pdf","file_id":"5299","access_level":"open_access","file_name":"IST-2018-970-v1+1_2018_Lanxin_Real-time_analysis.pdf","relation":"main_file","date_updated":"2020-07-14T12:46:29Z"}],"publist_id":"7381","type":"journal_article","publisher":"Bio-protocol","language":[{"iso":"eng"}],"project":[{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program","call_identifier":"H2020","grant_number":"665385"}],"year":"2018"},{"quality_controlled":"1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"10293"}],"link":[{"url":"https://ist.ac.at/en/news/no-cooperation-without-open-communication/","relation":"press_release","description":"News on IST Homepage"}]},"publication":"PNAS","issue":"48","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pubmed/30429320"}],"author":[{"first_name":"Christian","orcid":"0000-0001-5116-955X","full_name":"Hilbe, Christian","id":"2FDF8F3C-F248-11E8-B48F-1D18A9856A87","last_name":"Hilbe"},{"last_name":"Schmid","id":"38B437DE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6978-7329","full_name":"Schmid, Laura","first_name":"Laura"},{"id":"3F24CCC8-F248-11E8-B48F-1D18A9856A87","full_name":"Tkadlec, Josef","orcid":"0000-0002-1097-9684","first_name":"Josef","last_name":"Tkadlec"},{"last_name":"Chatterjee","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4561-241X","full_name":"Chatterjee, Krishnendu","first_name":"Krishnendu"},{"last_name":"Nowak","full_name":"Nowak, Martin","first_name":"Martin"}],"month":"11","status":"public","isi":1,"day":"27","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","doi":"10.1073/pnas.1810565115","external_id":{"pmid":["30429320"],"isi":["000451351000063"]},"date_published":"2018-11-27T00:00:00Z","citation":{"ista":"Hilbe C, Schmid L, Tkadlec J, Chatterjee K, Nowak M. 2018. Indirect reciprocity with private, noisy, and incomplete information. PNAS. 115(48), 12241–12246.","short":"C. Hilbe, L. Schmid, J. Tkadlec, K. Chatterjee, M. Nowak, PNAS 115 (2018) 12241–12246.","apa":"Hilbe, C., Schmid, L., Tkadlec, J., Chatterjee, K., &#38; Nowak, M. (2018). Indirect reciprocity with private, noisy, and incomplete information. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1810565115\">https://doi.org/10.1073/pnas.1810565115</a>","mla":"Hilbe, Christian, et al. “Indirect Reciprocity with Private, Noisy, and Incomplete Information.” <i>PNAS</i>, vol. 115, no. 48, National Academy of Sciences, 2018, pp. 12241–46, doi:<a href=\"https://doi.org/10.1073/pnas.1810565115\">10.1073/pnas.1810565115</a>.","chicago":"Hilbe, Christian, Laura Schmid, Josef Tkadlec, Krishnendu Chatterjee, and Martin Nowak. “Indirect Reciprocity with Private, Noisy, and Incomplete Information.” <i>PNAS</i>. National Academy of Sciences, 2018. <a href=\"https://doi.org/10.1073/pnas.1810565115\">https://doi.org/10.1073/pnas.1810565115</a>.","ama":"Hilbe C, Schmid L, Tkadlec J, Chatterjee K, Nowak M. Indirect reciprocity with private, noisy, and incomplete information. <i>PNAS</i>. 2018;115(48):12241-12246. doi:<a href=\"https://doi.org/10.1073/pnas.1810565115\">10.1073/pnas.1810565115</a>","ieee":"C. Hilbe, L. Schmid, J. Tkadlec, K. Chatterjee, and M. Nowak, “Indirect reciprocity with private, noisy, and incomplete information,” <i>PNAS</i>, vol. 115, no. 48. National Academy of Sciences, pp. 12241–12246, 2018."},"scopus_import":"1","ec_funded":1,"_id":"2","title":"Indirect reciprocity with private, noisy, and incomplete information","department":[{"_id":"KrCh"}],"date_created":"2018-12-11T11:44:05Z","publication_status":"published","pmid":1,"fulldoi":"https://doi.org/10.1073/pnas.1810565115","oa_version":"Submitted Version","oa":1,"article_processing_charge":"No","language":[{"iso":"eng"}],"project":[{"grant_number":"279307","call_identifier":"FP7","_id":"2581B60A-B435-11E9-9278-68D0E5697425","name":"Quantitative Graph Games: Theory and Applications"},{"grant_number":"P 23499-N23","call_identifier":"FWF","_id":"2584A770-B435-11E9-9278-68D0E5697425","name":"Modern Graph Algorithmic Techniques in Formal Verification"},{"name":"Rigorous Systems Engineering","_id":"25832EC2-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"S 11407_N23"},{"_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734"}],"year":"2018","type":"journal_article","publisher":"National Academy of Sciences","page":"12241-12246","volume":115,"abstract":[{"text":"Indirect reciprocity explores how humans act when their reputation is at stake, and which social norms they use to assess the actions of others. A crucial question in indirect reciprocity is which social norms can maintain stable cooperation in a society. Past research has highlighted eight such norms, called “leading-eight” strategies. This past research, however, is based on the assumption that all relevant information about other population members is publicly available and that everyone agrees on who is good or bad. Instead, here we explore the reputation dynamics when information is private and noisy. We show that under these conditions, most leading-eight strategies fail to evolve. Those leading-eight strategies that do evolve are unable to sustain full cooperation.Indirect reciprocity is a mechanism for cooperation based on shared moral systems and individual reputations. It assumes that members of a community routinely observe and assess each other and that they use this information to decide who is good or bad, and who deserves cooperation. When information is transmitted publicly, such that all community members agree on each other’s reputation, previous research has highlighted eight crucial moral systems. These “leading-eight” strategies can maintain cooperation and resist invasion by defectors. However, in real populations individuals often hold their own private views of others. Once two individuals disagree about their opinion of some third party, they may also see its subsequent actions in a different light. Their opinions may further diverge over time. Herein, we explore indirect reciprocity when information transmission is private and noisy. We find that in the presence of perception errors, most leading-eight strategies cease to be stable. Even if a leading-eight strategy evolves, cooperation rates may drop considerably when errors are common. Our research highlights the role of reliable information and synchronized reputations to maintain stable moral systems.","lang":"eng"}],"intvolume":"       115","date_updated":"2026-10-05T22:30:46Z"},{"publisher":"Institute of Science and Technology Austria","type":"dissertation","year":"2018","project":[{"name":"Transmembrane Transporters in Health and Disease","_id":"25473368-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"F03523"}],"language":[{"iso":"eng"}],"publist_id":"7434","date_updated":"2026-04-08T14:15:20Z","file":[{"file_name":"2018_Thesis_Tarlungeanu_source.docx","access_level":"closed","relation":"source_file","date_updated":"2021-02-11T23:30:15Z","checksum":"9f5231c96e0ad945040841a8630232da","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_id":"6217","embargo_to":"open_access","creator":"dernst","date_created":"2019-04-05T09:19:17Z","file_size":43684035},{"file_name":"2018_Thesis_Tarlungeanu.pdf","access_level":"open_access","date_updated":"2021-02-11T11:17:16Z","relation":"main_file","content_type":"application/pdf","checksum":"0c33c370aa2010df5c552db57a6d01e9","file_id":"6218","creator":"dernst","date_created":"2019-04-05T09:19:17Z","file_size":30511532,"embargo":"2018-03-15"}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"EM-Fac"},{"_id":"Bio"}],"pubrep_id":"992","abstract":[{"text":"Autism spectrum disorders (ASD) are a group of genetic disorders often overlapping with other neurological conditions. Despite the remarkable number of scientific breakthroughs of the last 100 years, the treatment of neurodevelopmental disorders (e.g. autism spectrum disorder, intellectual disability, epilepsy) remains a great challenge. Recent advancements in geno mics, like whole-exome or whole-genome sequencing, have enabled scientists to identify numerous mutations underlying neurodevelopmental disorders. Given the few hundred risk genes that were discovered, the etiological variability and the heterogeneous phenotypic outcomes, the need for genotype -along with phenotype- based diagnosis of individual patients becomes a requisite. Driven by this rationale, in a previous study our group described mutations, identified via whole - exome sequencing, in the gene BCKDK – encoding for a key regulator of branched chain amin o acid (BCAA) catabolism - as a cause of ASD. Following up on the role of BCAAs, in the study described here we show that the solute carrier transporter 7a5 (SLC7A5), a large neutral amino acid transporter localized mainly at the blood brain barrier (BBB), has an essential role in maintaining normal levels of brain BCAAs. In mice, deletion of Slc7a5 from the endothelial cells of the BBB leads to atypical brain amino acid profile, abnormal mRNA translation and severe neurolo gical abnormalities. Additionally, deletion of Slc7a5 from the neural progenitor cell population leads to microcephaly. Interestingly, we demonstrate that BCAA intracerebroventricular administration ameliorates abnormal behaviors in adult mutant mice. Furthermore, whole - exome sequencing of patients diagnosed with neurological dis o r ders helped us identify several patients with autistic traits, microcephaly and motor delay carrying deleterious homozygous mutations in the SLC7A5 gene. In conclusion, our data elucidate a neurological syndrome defined by SLC7A5 mutations and support an essential role for t he BCAA s in human bra in function. Together with r ecent studies (described in chapter two) that have successfully made the transition into clinical practice, our findings on the role of B CAAs might have a crucial impact on the development of novel individualized therapeutic strategies for ASD. ","lang":"eng"}],"page":"88","publication_identifier":{"issn":["2663-337X"]},"date_created":"2018-12-11T11:46:14Z","department":[{"_id":"GaNo"}],"degree_awarded":"PhD","title":"The branched chain amino acids in autism spectrum disorders ","_id":"395","article_processing_charge":"No","oa":1,"oa_version":"Published Version","fulldoi":"https://doi.org/10.15479/AT:ISTA:th_992","publication_status":"published","OA_place":"publisher","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_published":"2018-03-01T00:00:00Z","file_date_updated":"2021-02-11T23:30:15Z","has_accepted_license":"1","corr_author":"1","citation":{"ista":"Tarlungeanu D-C. 2018. The branched chain amino acids in autism spectrum disorders . Institute of Science and Technology Austria.","apa":"Tarlungeanu, D.-C. (2018). <i>The branched chain amino acids in autism spectrum disorders </i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th_992\">https://doi.org/10.15479/AT:ISTA:th_992</a>","short":"D.-C. Tarlungeanu, The Branched Chain Amino Acids in Autism Spectrum Disorders , Institute of Science and Technology Austria, 2018.","mla":"Tarlungeanu, Dora-Clara. <i>The Branched Chain Amino Acids in Autism Spectrum Disorders </i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_992\">10.15479/AT:ISTA:th_992</a>.","chicago":"Tarlungeanu, Dora-Clara. “The Branched Chain Amino Acids in Autism Spectrum Disorders .” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:th_992\">https://doi.org/10.15479/AT:ISTA:th_992</a>.","ama":"Tarlungeanu D-C. The branched chain amino acids in autism spectrum disorders . 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_992\">10.15479/AT:ISTA:th_992</a>","ieee":"D.-C. Tarlungeanu, “The branched chain amino acids in autism spectrum disorders ,” Institute of Science and Technology Austria, 2018."},"month":"03","ddc":["570","616"],"status":"public","author":[{"last_name":"Tarlungeanu","full_name":"Tarlungeanu, Dora-Clara","id":"2ABCE612-F248-11E8-B48F-1D18A9856A87","first_name":"Dora-Clara"}],"related_material":{"record":[{"id":"1183","relation":"part_of_dissertation","status":"public"}]},"alternative_title":["ISTA Thesis"],"doi":"10.15479/AT:ISTA:th_992","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","day":"01","supervisor":[{"first_name":"Gaia","orcid":"0000-0002-7673-7178","full_name":"Novarino, Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","last_name":"Novarino"}]},{"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_published":"2018-04-12T00:00:00Z","acknowledgement":"First of all I would like to thank Michael Sixt for giving me the opportunity to work in \r\nhis group and for his support throughout the years. He is a truly inspiring person and \r\nthe  best  boss  one  can  imagine.  I  would  also  like  to  thank  all  current  and  past \r\nmembers of the Sixt group for their help and the great working atmosphere in the lab. \r\nIt is a true privilege to work with such a bright, funny and friendly group of people and \r\nI’m  proud  that  I  could  be  part  of  it.  Furthermore,  I  would  like  to  say  ‘thank  you’  to Daria Siekhaus for all the meetings and discussion we had throughout the years \r\nand to  Federica  Benvenuti  for  being  part  of  my  committee.  I  am  also  grateful  to  Jack \r\nMerrin  in  the  nanofabrication  facility  and  all  the  people  working  in  the  bioimaging-\r\n, the electron microscopy- and the preclinical facilities.","file_date_updated":"2021-02-11T23:30:17Z","has_accepted_license":"1","corr_author":"1","citation":{"ama":"Leithner AF. Branched actin networks in dendritic cell biology. 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_998\">10.15479/AT:ISTA:th_998</a>","ieee":"A. F. Leithner, “Branched actin networks in dendritic cell biology,” Institute of Science and Technology Austria, 2018.","mla":"Leithner, Alexander F. <i>Branched Actin Networks in Dendritic Cell Biology</i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_998\">10.15479/AT:ISTA:th_998</a>.","ista":"Leithner AF. 2018. Branched actin networks in dendritic cell biology. Institute of Science and Technology Austria.","short":"A.F. Leithner, Branched Actin Networks in Dendritic Cell Biology, Institute of Science and Technology Austria, 2018.","apa":"Leithner, A. F. (2018). <i>Branched actin networks in dendritic cell biology</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th_998\">https://doi.org/10.15479/AT:ISTA:th_998</a>","chicago":"Leithner, Alexander F. “Branched Actin Networks in Dendritic Cell Biology.” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:th_998\">https://doi.org/10.15479/AT:ISTA:th_998</a>."},"author":[{"id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1073-744X","full_name":"Leithner, Alexander F","first_name":"Alexander F","last_name":"Leithner"}],"month":"04","ddc":["571","599","610"],"status":"public","related_material":{"record":[{"id":"1321","status":"public","relation":"part_of_dissertation"}]},"alternative_title":["ISTA Thesis"],"doi":"10.15479/AT:ISTA:th_998","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","day":"12","supervisor":[{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt"}],"type":"dissertation","publisher":"Institute of Science and Technology Austria","language":[{"iso":"eng"}],"year":"2018","publist_id":"7542","date_updated":"2025-09-22T08:27:34Z","acknowledged_ssus":[{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"EM-Fac"}],"file":[{"file_name":"PhD_thesis_AlexLeithner_final_version.docx","access_level":"closed","relation":"source_file","date_updated":"2021-02-11T23:30:17Z","checksum":"d5e3edbac548c26c1fa43a4b37a54a4c","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_id":"6219","embargo_to":"open_access","creator":"dernst","date_created":"2019-04-05T09:23:11Z","file_size":29027671},{"creator":"dernst","file_id":"6220","content_type":"application/pdf","checksum":"071f7476db29e41146824ebd0697cb10","date_updated":"2021-02-11T11:17:16Z","relation":"main_file","file_name":"PhD_thesis_AlexLeithner.pdf","access_level":"open_access","embargo":"2019-04-15","file_size":66045341,"date_created":"2019-04-05T09:23:11Z"}],"pubrep_id":"998","abstract":[{"text":"In the here presented thesis, we explore the role of branched actin networks in cell migration and antigen presentation, the two most relevant processes in dendritic cell biology. Branched actin networks construct lamellipodial protrusions at the leading edge of migrating cells. These are typically seen as adhesive structures, which mediate force transduction to the extracellular matrix that leads to forward locomotion. We ablated Arp2/3 nucleation promoting factor WAVE in DCs and found that the resulting cells lack lamellipodial protrusions. Instead, depending on the maturation state, one or multiple filopodia were formed. By challenging these cells in a variety of migration assays we found that lamellipodial protrusions are dispensable for the locomotion of leukocytes and actually dampen the speed of migration. However, lamellipodia are critically required to negotiate complex environments that DCs experience while they travel to the next draining lymph node. Taken together our results suggest that leukocyte lamellipodia have rather a sensory- than a force transducing function. Furthermore, we show for the first time structure and dynamics of dendritic cell F-actin at the immunological synapse with naïve T cells. Dendritic cell F-actin appears as dynamic foci that are nucleated by the Arp2/3 complex. WAVE ablated dendritic cells show increased membrane tension, leading to an altered ultrastructure of the immunological synapse and severe T cell priming defects. These results point towards a previously unappreciated role of the cellular mechanics of dendritic cells in T cell activation. Additionally, we present a novel cell culture based system for the differentiation of dendritic cells from conditionally immortalized hematopoietic precursors. These precursor cells are genetically tractable via the CRISPR/Cas9 system while they retain their ability to differentiate into highly migratory dendritic cells and other immune cells. This will foster the study of all aspects of dendritic cell biology and beyond. ","lang":"eng"}],"page":"99","publication_identifier":{"issn":["2663-337X"]},"date_created":"2018-12-11T11:45:49Z","degree_awarded":"PhD","department":[{"_id":"MiSi"}],"_id":"323","title":"Branched actin networks in dendritic cell biology","article_processing_charge":"No","oa":1,"fulldoi":"https://doi.org/10.15479/AT:ISTA:th_998","oa_version":"Published Version","publication_status":"published"},{"intvolume":"        87","date_updated":"2026-10-05T22:30:49Z","abstract":[{"text":"Plant hormones as signalling molecules play an essential role in the control of plant growth and development. Typically, sites of hormonal action are usually distant from the site of biosynthesis thus relying on efficient transport mechanisms. Over the last decades, molecular identification of proteins and protein complexes involved in hormonal transport has started. Advanced screens for genes involved in hormonal transport in combination with transport assays using heterologous systems such as yeast, insect, or tobacco BY2 cells or Xenopus oocytes provided important insights into mechanisms underlying distribution of hormones in plant body and led to identification of principal transporters for each hormone. This review gives a short overview of the mechanisms of hormonal transport and transporters identified in Arabidopsis thaliana.","lang":"eng"}],"volume":87,"page":"115 - 138","publisher":"Elsevier","type":"journal_article","year":"2018","language":[{"iso":"eng"}],"publist_id":"8007","article_processing_charge":"No","oa_version":"None","fulldoi":"https://doi.org/10.1016/bs.abr.2018.09.007","publication_status":"published","date_created":"2018-12-11T11:44:20Z","department":[{"_id":"EvBe"}],"title":"Transporters and mechanisms of hormone transport in arabidopsis","_id":"47","scopus_import":"1","citation":{"ista":"Abualia R, Benková E, Lacombe B. 2018. Transporters and mechanisms of hormone transport in arabidopsis. Advances in Botanical Research. 87, 115–138.","apa":"Abualia, R., Benková, E., &#38; Lacombe, B. (2018). Transporters and mechanisms of hormone transport in arabidopsis. <i>Advances in Botanical Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">https://doi.org/10.1016/bs.abr.2018.09.007</a>","short":"R. Abualia, E. Benková, B. Lacombe, Advances in Botanical Research 87 (2018) 115–138.","mla":"Abualia, Rashed, et al. “Transporters and Mechanisms of Hormone Transport in Arabidopsis.” <i>Advances in Botanical Research</i>, vol. 87, Elsevier, 2018, pp. 115–38, doi:<a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">10.1016/bs.abr.2018.09.007</a>.","chicago":"Abualia, Rashed, Eva Benková, and Benoît Lacombe. “Transporters and Mechanisms of Hormone Transport in Arabidopsis.” <i>Advances in Botanical Research</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">https://doi.org/10.1016/bs.abr.2018.09.007</a>.","ama":"Abualia R, Benková E, Lacombe B. Transporters and mechanisms of hormone transport in arabidopsis. <i>Advances in Botanical Research</i>. 2018;87:115-138. doi:<a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">10.1016/bs.abr.2018.09.007</a>","ieee":"R. Abualia, E. Benková, and B. Lacombe, “Transporters and mechanisms of hormone transport in arabidopsis,” <i>Advances in Botanical Research</i>, vol. 87. Elsevier, pp. 115–138, 2018."},"date_published":"2018-01-01T00:00:00Z","external_id":{"isi":["000453657800006"]},"doi":"10.1016/bs.abr.2018.09.007","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","day":"01","isi":1,"month":"01","status":"public","author":[{"last_name":"Abualia","first_name":"Rashed","id":"4827E134-F248-11E8-B48F-1D18A9856A87","full_name":"Abualia, Rashed","orcid":"0000-0002-9357-9415"},{"last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"first_name":"Benoît","full_name":"Lacombe, Benoît","last_name":"Lacombe"}],"publication":"Advances in Botanical Research","quality_controlled":"1","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"10303"}]}},{"day":"29","doi":"10.1371/journal.pgen.1007177","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"7172"},{"id":"1127","relation":"dissertation_contains","status":"public"},{"id":"8822","status":"public","relation":"dissertation_contains"}]},"quality_controlled":"1","publication":"PLoS Genetics","issue":"1","status":"public","ddc":["581"],"isi":1,"month":"01","author":[{"last_name":"Prat","id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87","full_name":"Prat, Tomas","first_name":"Tomas"},{"last_name":"Hajny","full_name":"Hajny, Jakub","orcid":"0000-0003-2140-7195","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","first_name":"Jakub"},{"last_name":"Grunewald","full_name":"Grunewald, Wim","first_name":"Wim"},{"last_name":"Vasileva","first_name":"Mina K","full_name":"Vasileva, Mina K","id":"3407EB18-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Gergely","full_name":"Molnar, Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","last_name":"Molnar"},{"full_name":"Tejos, Ricardo","first_name":"Ricardo","last_name":"Tejos"},{"last_name":"Schmid","full_name":"Schmid, Markus","first_name":"Markus"},{"last_name":"Sauer","full_name":"Sauer, Michael","first_name":"Michael"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"citation":{"chicago":"Prat, Tomas, Jakub Hajny, Wim Grunewald, Mina K Vasileva, Gergely Molnar, Ricardo Tejos, Markus Schmid, Michael Sauer, and Jiří Friml. “WRKY23 Is a Component of the Transcriptional Network Mediating Auxin Feedback on PIN Polarity.” <i>PLoS Genetics</i>. Public Library of Science, 2018. <a href=\"https://doi.org/10.1371/journal.pgen.1007177\">https://doi.org/10.1371/journal.pgen.1007177</a>.","short":"T. Prat, J. Hajny, W. Grunewald, M.K. Vasileva, G. Molnar, R. Tejos, M. Schmid, M. Sauer, J. Friml, PLoS Genetics 14 (2018).","ista":"Prat T, Hajny J, Grunewald W, Vasileva MK, Molnar G, Tejos R, Schmid M, Sauer M, Friml J. 2018. WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity. PLoS Genetics. 14(1).","apa":"Prat, T., Hajny, J., Grunewald, W., Vasileva, M. K., Molnar, G., Tejos, R., … Friml, J. (2018). WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity. <i>PLoS Genetics</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pgen.1007177\">https://doi.org/10.1371/journal.pgen.1007177</a>","mla":"Prat, Tomas, et al. “WRKY23 Is a Component of the Transcriptional Network Mediating Auxin Feedback on PIN Polarity.” <i>PLoS Genetics</i>, vol. 14, no. 1, Public Library of Science, 2018, doi:<a href=\"https://doi.org/10.1371/journal.pgen.1007177\">10.1371/journal.pgen.1007177</a>.","ama":"Prat T, Hajny J, Grunewald W, et al. WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity. <i>PLoS Genetics</i>. 2018;14(1). doi:<a href=\"https://doi.org/10.1371/journal.pgen.1007177\">10.1371/journal.pgen.1007177</a>","ieee":"T. Prat <i>et al.</i>, “WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity,” <i>PLoS Genetics</i>, vol. 14, no. 1. Public Library of Science, 2018."},"scopus_import":"1","ec_funded":1,"external_id":{"isi":["000423718600034"]},"corr_author":"1","has_accepted_license":"1","file_date_updated":"2020-07-14T12:46:30Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_published":"2018-01-29T00:00:00Z","publication_status":"published","oa_version":"Published Version","fulldoi":"https://doi.org/10.1371/journal.pgen.1007177","oa":1,"article_processing_charge":"Yes","title":"WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity","_id":"449","department":[{"_id":"JiFr"}],"date_created":"2018-12-11T11:46:32Z","volume":14,"pubrep_id":"967","abstract":[{"lang":"eng","text":"Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17- and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain- and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development."}],"file":[{"file_size":24709062,"date_created":"2018-12-12T10:10:52Z","creator":"system","file_id":"4843","content_type":"application/pdf","checksum":"0276d66788ec076f4924164a39e6a712","relation":"main_file","date_updated":"2020-07-14T12:46:30Z","access_level":"open_access","file_name":"IST-2018-967-v1+1_journal.pgen.1007177.pdf"}],"intvolume":"        14","date_updated":"2026-10-05T22:30:53Z","publist_id":"7373","year":"2018","project":[{"grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7"}],"language":[{"iso":"eng"}],"publisher":"Public Library of Science","type":"journal_article"},{"citation":{"ieee":"P. Grones <i>et al.</i>, “PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism,” <i>Scientific Reports</i>, vol. 8, no. 1. Springer, 2018.","ama":"Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. <i>Scientific Reports</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.1038/s41598-018-28188-1\">10.1038/s41598-018-28188-1</a>","chicago":"Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann, Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific Reports</i>. Springer, 2018. <a href=\"https://doi.org/10.1038/s41598-018-28188-1\">https://doi.org/10.1038/s41598-018-28188-1</a>.","short":"P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J. Friml, Scientific Reports 8 (2018).","ista":"Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. Scientific Reports. 8(1), 10279.","apa":"Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J., &#38; Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. <i>Scientific Reports</i>. Springer. <a href=\"https://doi.org/10.1038/s41598-018-28188-1\">https://doi.org/10.1038/s41598-018-28188-1</a>","mla":"Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific Reports</i>, vol. 8, no. 1, 10279, Springer, 2018, doi:<a href=\"https://doi.org/10.1038/s41598-018-28188-1\">10.1038/s41598-018-28188-1</a>."},"scopus_import":"1","ec_funded":1,"external_id":{"isi":["000437673200053"]},"has_accepted_license":"1","file_date_updated":"2020-07-14T12:45:20Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_published":"2018-07-06T00:00:00Z","day":"06","doi":"10.1038/s41598-018-28188-1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"8822"}]},"quality_controlled":"1","publication":"Scientific Reports","issue":"1","author":[{"last_name":"Grones","first_name":"Peter","full_name":"Grones, Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Abas","first_name":"Melinda F","id":"3CFB3B1C-F248-11E8-B48F-1D18A9856A87","full_name":"Abas, Melinda F"},{"last_name":"Hajny","first_name":"Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2140-7195","full_name":"Hajny, Jakub"},{"first_name":"Angharad","full_name":"Jones, Angharad","last_name":"Jones"},{"last_name":"Waidmann","full_name":"Waidmann, Sascha","first_name":"Sascha"},{"last_name":"Kleine Vehn","full_name":"Kleine Vehn, Jürgen","first_name":"Jürgen"},{"last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"month":"07","isi":1,"status":"public","ddc":["581"],"article_number":"10279","volume":8,"abstract":[{"text":"Intercellular distribution of the plant hormone auxin largely depends on the polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters. PIN polarity switches in response to different developmental and environmental signals have been shown to redirect auxin fluxes mediating certain developmental responses. PIN phosphorylation at different sites and by different kinases is crucial for PIN function. Here we investigate the role of PIN phosphorylation during gravitropic response. Loss- and gain-of-function mutants in PINOID and related kinases but not in D6PK kinase as well as mutations mimicking constitutive dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements in response to gravity and during feed-back regulation by auxin itself. Thus PIN phosphorylation, besides regulating transport activity and apical-basal targeting, is also important for the rapid polarity switches in response to environmental and endogenous signals.","lang":"eng"}],"file":[{"relation":"main_file","date_updated":"2020-07-14T12:45:20Z","file_name":"2018_ScientificReports_Grones.pdf","access_level":"open_access","creator":"dernst","file_id":"5714","content_type":"application/pdf","checksum":"266b03f4fb8198e83141617aaa99dcab","file_size":2413876,"date_created":"2018-12-17T15:38:56Z"}],"date_updated":"2026-10-05T22:30:53Z","intvolume":"         8","publist_id":"7729","language":[{"iso":"eng"}],"project":[{"grant_number":"282300","call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425"},{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742985"}],"year":"2018","type":"journal_article","publisher":"Springer","publication_status":"published","fulldoi":"https://doi.org/10.1038/s41598-018-28188-1","oa_version":"Published Version","oa":1,"article_processing_charge":"No","_id":"191","title":"PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"date_created":"2018-12-11T11:45:06Z"},{"date_created":"2018-12-11T11:46:16Z","department":[{"_id":"MiSi"}],"_id":"402","title":"Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice","article_processing_charge":"No","oa":1,"pmid":1,"fulldoi":"https://doi.org/10.1126/science.aal3662","oa_version":"Published Version","publication_status":"published","type":"journal_article","publisher":"American Association for the Advancement of Science","language":[{"iso":"eng"}],"project":[{"call_identifier":"FWF","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","grant_number":"Y 564-B12"},{"grant_number":"281556","call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","_id":"25A603A2-B435-11E9-9278-68D0E5697425"}],"year":"2018","publist_id":"7428","date_updated":"2026-10-05T22:30:55Z","intvolume":"       359","acknowledged_ssus":[{"_id":"Bio"}],"abstract":[{"lang":"eng","text":"During metastasis, malignant cells escape the primary tumor, intravasate lymphatic vessels, and reach draining sentinel lymph nodes before they colonize distant organs via the blood circulation. Although lymph node metastasis in cancer patients correlates with poor prognosis, evidence is lacking as to whether and how tumor cells enter the bloodstream via lymph nodes. To investigate this question, we delivered carcinoma cells into the lymph nodes of mice by microinfusing the cells into afferent lymphatic vessels. We found that tumor cells rapidly infiltrated the lymph node parenchyma, invaded blood vessels, and seeded lung metastases without involvement of the thoracic duct. These results suggest that the lymph node blood vessels can serve as an exit route for systemic dissemination of cancer cells in experimental mouse models. Whether this form of tumor cell spreading occurs in cancer patients remains to be determined."}],"page":"1408 - 1411","volume":359,"author":[{"last_name":"Brown","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","full_name":"Brown, Markus","first_name":"Markus"},{"last_name":"Assen","orcid":"0000-0003-3470-6119","full_name":"Assen, Frank P","id":"3A8E7F24-F248-11E8-B48F-1D18A9856A87","first_name":"Frank P"},{"id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1073-744X","full_name":"Leithner, Alexander F","first_name":"Alexander F","last_name":"Leithner"},{"last_name":"Abe","first_name":"Jun","full_name":"Abe, Jun"},{"last_name":"Schachner","first_name":"Helga","full_name":"Schachner, Helga"},{"full_name":"Asfour, Gabriele","first_name":"Gabriele","last_name":"Asfour"},{"first_name":"Zsuzsanna","full_name":"Bagó Horváth, Zsuzsanna","last_name":"Bagó Horváth"},{"last_name":"Stein","first_name":"Jens","full_name":"Stein, Jens"},{"last_name":"Uhrin","full_name":"Uhrin, Pavel","first_name":"Pavel"},{"last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K"},{"full_name":"Kerjaschki, Dontscho","first_name":"Dontscho","last_name":"Kerjaschki"}],"month":"03","isi":1,"status":"public","ddc":["570"],"main_file_link":[{"url":"https://doi.org/10.1126/science.aal3662","open_access":"1"}],"issue":"6382","publication":"Science","quality_controlled":"1","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"6947"}]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1126/science.aal3662","article_type":"original","day":"23","date_published":"2018-03-23T00:00:00Z","acknowledgement":"M.B. was supported by the Cell Communication in Health and Disease graduate study program of the Austrian Science Fund (FWF) and the Medical University of Vienna. M.S. was supported by the European Research Council (grant ERC GA 281556) and an FWF START award.\r\nWe thank C. Moussion for establishing the intralymphatic injection at IST Austria and for providing anti-PNAd hybridoma supernatant, R. Förster and A. Braun for sharing the intralymphatic injection technology, K. Vaahtomeri for the lentiviral constructs, M. Hons for establishing in vivo multiphoton imaging, the Sixt lab for intellectual input, M. Schunn for help with the design of the in vivo experiments, F. Langer for technical assistance with the in vivo experiments, the bioimaging facility of IST Austria for support, and R. Efferl for providing the CT26 cell line.","corr_author":"1","external_id":{"isi":["000428043600047"],"pmid":["29567714"]},"ec_funded":1,"scopus_import":"1","citation":{"ieee":"M. Brown <i>et al.</i>, “Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice,” <i>Science</i>, vol. 359, no. 6382. American Association for the Advancement of Science, pp. 1408–1411, 2018.","ama":"Brown M, Assen FP, Leithner AF, et al. Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice. <i>Science</i>. 2018;359(6382):1408-1411. doi:<a href=\"https://doi.org/10.1126/science.aal3662\">10.1126/science.aal3662</a>","mla":"Brown, Markus, et al. “Lymph Node Blood Vessels Provide Exit Routes for Metastatic Tumor Cell Dissemination in Mice.” <i>Science</i>, vol. 359, no. 6382, American Association for the Advancement of Science, 2018, pp. 1408–11, doi:<a href=\"https://doi.org/10.1126/science.aal3662\">10.1126/science.aal3662</a>.","short":"M. Brown, F.P. Assen, A.F. Leithner, J. Abe, H. Schachner, G. Asfour, Z. Bagó Horváth, J. Stein, P. Uhrin, M.K. Sixt, D. Kerjaschki, Science 359 (2018) 1408–1411.","apa":"Brown, M., Assen, F. P., Leithner, A. F., Abe, J., Schachner, H., Asfour, G., … Kerjaschki, D. (2018). Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aal3662\">https://doi.org/10.1126/science.aal3662</a>","ista":"Brown M, Assen FP, Leithner AF, Abe J, Schachner H, Asfour G, Bagó Horváth Z, Stein J, Uhrin P, Sixt MK, Kerjaschki D. 2018. Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice. Science. 359(6382), 1408–1411.","chicago":"Brown, Markus, Frank P Assen, Alexander F Leithner, Jun Abe, Helga Schachner, Gabriele Asfour, Zsuzsanna Bagó Horváth, et al. “Lymph Node Blood Vessels Provide Exit Routes for Metastatic Tumor Cell Dissemination in Mice.” <i>Science</i>. American Association for the Advancement of Science, 2018. <a href=\"https://doi.org/10.1126/science.aal3662\">https://doi.org/10.1126/science.aal3662</a>."}},{"publist_id":"7988","publisher":"Schloss Dagstuhl - Leibniz-Zentrum für Informatik","type":"conference","year":"2018","project":[{"grant_number":"ICT15-003","name":"Efficient Algorithms for Computer Aided Verification","_id":"25892FC0-B435-11E9-9278-68D0E5697425"},{"grant_number":"279307","call_identifier":"FP7","_id":"2581B60A-B435-11E9-9278-68D0E5697425","name":"Quantitative Graph Games: Theory and Applications"},{"call_identifier":"FWF","name":"Rigorous Systems Engineering","_id":"25832EC2-B435-11E9-9278-68D0E5697425","grant_number":"S 11407_N23"},{"name":"Quantitative Game-theoretic Analysis of Blockchain Applications and Smart Contracts","_id":"266EEEC0-B435-11E9-9278-68D0E5697425"}],"language":[{"iso":"eng"}],"arxiv":1,"abstract":[{"lang":"eng","text":"Crypto-currencies are digital assets designed to work as a medium of exchange, e.g., Bitcoin, but they are susceptible to attacks (dishonest behavior of participants). A framework for the analysis of attacks in crypto-currencies requires (a) modeling of game-theoretic aspects to analyze incentives for deviation from honest behavior; (b) concurrent interactions between participants; and (c) analysis of long-term monetary gains. Traditional game-theoretic approaches for the analysis of security protocols consider either qualitative temporal properties such as safety and termination, or the very special class of one-shot (stateless) games. However, to analyze general attacks on protocols for crypto-currencies, both stateful analysis and quantitative objectives are necessary. In this work our main contributions are as follows: (a) we show how a class of concurrent mean-payo games, namely ergodic games, can model various attacks that arise naturally in crypto-currencies; (b) we present the first practical implementation of algorithms for ergodic games that scales to model realistic problems for crypto-currencies; and (c) we present experimental results showing that our framework can handle games with thousands of states and millions of transitions."}],"volume":118,"article_number":"11","intvolume":"       118","date_updated":"2026-10-05T22:30:58Z","conference":{"name":"CONCUR: Conference on Concurrency Theory","end_date":"2018-09-07","start_date":"2018-09-04","location":"Beijing, China"},"file":[{"file_size":1078309,"date_created":"2018-12-17T12:08:00Z","file_name":"2018_CONCUR_Chatterjee.pdf","access_level":"open_access","date_updated":"2020-07-14T12:47:34Z","relation":"main_file","creator":"dernst","checksum":"68a055b1aaa241cc38375083cf832a7d","content_type":"application/pdf","file_id":"5696"}],"department":[{"_id":"KrCh"}],"title":"Ergodic mean-payoff games for the analysis of attacks in crypto-currencies","_id":"66","publication_identifier":{"isbn":["978-3-95977-087-3"]},"date_created":"2018-12-11T11:44:27Z","oa_version":"Published Version","fulldoi":"https://doi.org/10.4230/LIPIcs.CONCUR.2018.11","publication_status":"published","article_processing_charge":"No","oa":1,"external_id":{"arxiv":["1806.03108"]},"date_published":"2018-09-01T00:00:00Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"file_date_updated":"2020-07-14T12:47:34Z","has_accepted_license":"1","citation":{"ieee":"K. Chatterjee, A. K. Goharshady, R. Ibsen-Jensen, and Y. Velner, “Ergodic mean-payoff games for the analysis of attacks in crypto-currencies,” presented at the CONCUR: Conference on Concurrency Theory, Beijing, China, 2018, vol. 118.","ama":"Chatterjee K, Goharshady AK, Ibsen-Jensen R, Velner Y. Ergodic mean-payoff games for the analysis of attacks in crypto-currencies. In: Vol 118. Schloss Dagstuhl - Leibniz-Zentrum für Informatik; 2018. doi:<a href=\"https://doi.org/10.4230/LIPIcs.CONCUR.2018.11\">10.4230/LIPIcs.CONCUR.2018.11</a>","chicago":"Chatterjee, Krishnendu, Amir Kafshdar Goharshady, Rasmus Ibsen-Jensen, and Yaron Velner. “Ergodic Mean-Payoff Games for the Analysis of Attacks in Crypto-Currencies,” Vol. 118. Schloss Dagstuhl - Leibniz-Zentrum für Informatik, 2018. <a href=\"https://doi.org/10.4230/LIPIcs.CONCUR.2018.11\">https://doi.org/10.4230/LIPIcs.CONCUR.2018.11</a>.","ista":"Chatterjee K, Goharshady AK, Ibsen-Jensen R, Velner Y. 2018. Ergodic mean-payoff games for the analysis of attacks in crypto-currencies. CONCUR: Conference on Concurrency Theory, LIPIcs, vol. 118, 11.","apa":"Chatterjee, K., Goharshady, A. K., Ibsen-Jensen, R., &#38; Velner, Y. (2018). Ergodic mean-payoff games for the analysis of attacks in crypto-currencies (Vol. 118). Presented at the CONCUR: Conference on Concurrency Theory, Beijing, China: Schloss Dagstuhl - Leibniz-Zentrum für Informatik. <a href=\"https://doi.org/10.4230/LIPIcs.CONCUR.2018.11\">https://doi.org/10.4230/LIPIcs.CONCUR.2018.11</a>","short":"K. Chatterjee, A.K. Goharshady, R. Ibsen-Jensen, Y. Velner, in:, Schloss Dagstuhl - Leibniz-Zentrum für Informatik, 2018.","mla":"Chatterjee, Krishnendu, et al. <i>Ergodic Mean-Payoff Games for the Analysis of Attacks in Crypto-Currencies</i>. Vol. 118, 11, Schloss Dagstuhl - Leibniz-Zentrum für Informatik, 2018, doi:<a href=\"https://doi.org/10.4230/LIPIcs.CONCUR.2018.11\">10.4230/LIPIcs.CONCUR.2018.11</a>."},"ec_funded":1,"scopus_import":"1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"8934"}]},"quality_controlled":"1","ddc":["000"],"month":"09","status":"public","author":[{"first_name":"Krishnendu","full_name":"Chatterjee, Krishnendu","orcid":"0000-0002-4561-241X","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","last_name":"Chatterjee"},{"first_name":"Amir","id":"391365CE-F248-11E8-B48F-1D18A9856A87","full_name":"Goharshady, Amir","orcid":"0000-0003-1702-6584","last_name":"Goharshady"},{"first_name":"Rasmus","orcid":"0000-0003-4783-0389","full_name":"Ibsen-Jensen, Rasmus","id":"3B699956-F248-11E8-B48F-1D18A9856A87","last_name":"Ibsen-Jensen"},{"full_name":"Velner, Yaron","first_name":"Yaron","last_name":"Velner"}],"day":"01","alternative_title":["LIPIcs"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.4230/LIPIcs.CONCUR.2018.11"},{"external_id":{"isi":["000481634500196"],"arxiv":["1805.09104"]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)"},"date_published":"2018-09-01T00:00:00Z","file_date_updated":"2020-07-14T12:47:27Z","has_accepted_license":"1","citation":{"chicago":"Goharshady, Amir Kafshdar, Ali Behrouz, and Krishnendu Chatterjee. “Secure Credit Reporting on the Blockchain.” In <i>Proceedings of the IEEE International Conference on Blockchain</i>, 1343–48. IEEE, 2018. <a href=\"https://doi.org/10.1109/Cybermatics_2018.2018.00231\">https://doi.org/10.1109/Cybermatics_2018.2018.00231</a>.","short":"A.K. Goharshady, A. Behrouz, K. Chatterjee, in:, Proceedings of the IEEE International Conference on Blockchain, IEEE, 2018, pp. 1343–1348.","apa":"Goharshady, A. K., Behrouz, A., &#38; Chatterjee, K. (2018). Secure Credit Reporting on the Blockchain. In <i>Proceedings of the IEEE International Conference on Blockchain</i> (pp. 1343–1348). Halifax, Canada: IEEE. <a href=\"https://doi.org/10.1109/Cybermatics_2018.2018.00231\">https://doi.org/10.1109/Cybermatics_2018.2018.00231</a>","ista":"Goharshady AK, Behrouz A, Chatterjee K. 2018. Secure Credit Reporting on the Blockchain. Proceedings of the IEEE International Conference on Blockchain. IEEE International Conference on Blockchain, 1343–1348.","mla":"Goharshady, Amir Kafshdar, et al. “Secure Credit Reporting on the Blockchain.” <i>Proceedings of the IEEE International Conference on Blockchain</i>, IEEE, 2018, pp. 1343–48, doi:<a href=\"https://doi.org/10.1109/Cybermatics_2018.2018.00231\">10.1109/Cybermatics_2018.2018.00231</a>.","ama":"Goharshady AK, Behrouz A, Chatterjee K. Secure Credit Reporting on the Blockchain. In: <i>Proceedings of the IEEE International Conference on Blockchain</i>. IEEE; 2018:1343-1348. doi:<a href=\"https://doi.org/10.1109/Cybermatics_2018.2018.00231\">10.1109/Cybermatics_2018.2018.00231</a>","ieee":"A. K. Goharshady, A. Behrouz, and K. Chatterjee, “Secure Credit Reporting on the Blockchain,” in <i>Proceedings of the IEEE International Conference on Blockchain</i>, Halifax, Canada, 2018, pp. 1343–1348."},"ec_funded":1,"scopus_import":"1","publication":"Proceedings of the IEEE International Conference on Blockchain","quality_controlled":"1","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"8934"}]},"author":[{"orcid":"0000-0003-1702-6584","full_name":"Goharshady, Amir Kafshdar","id":"391365CE-F248-11E8-B48F-1D18A9856A87","first_name":"Amir Kafshdar","last_name":"Goharshady"},{"last_name":"Behrouz","full_name":"Behrouz, Ali","first_name":"Ali"},{"last_name":"Chatterjee","first_name":"Krishnendu","full_name":"Chatterjee, Krishnendu","orcid":"0000-0002-4561-241X","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87"}],"status":"public","month":"09","isi":1,"ddc":["000"],"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","day":"01","doi":"10.1109/Cybermatics_2018.2018.00231","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","type":"conference","publisher":"IEEE","language":[{"iso":"eng"}],"project":[{"name":"Efficient Algorithms for Computer Aided Verification","_id":"25892FC0-B435-11E9-9278-68D0E5697425","grant_number":"ICT15-003"},{"_id":"266EEEC0-B435-11E9-9278-68D0E5697425","name":"Quantitative Game-theoretic Analysis of Blockchain Applications and Smart Contracts"},{"name":"Quantitative Graph Games: Theory and Applications","_id":"2581B60A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"279307"},{"name":"Rigorous Systems Engineering","_id":"25832EC2-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"S 11407_N23"}],"year":"2018","abstract":[{"text":"We  present  a  secure  approach  for  maintaining  andreporting  credit  history  records  on  the  Blockchain.  Our  ap-proach  removes  third-parties  such  as  credit  reporting  agen-cies  from  the  lending  process  and  replaces  them  with  smartcontracts.  This  allows  customers  to  interact  directly  with  thelenders  or  banks  while  ensuring  the  integrity,  unmalleabilityand  privacy  of  their  credit  data.  Additionally,  each  customerhas  full  control  over  complete  or  selective  disclosure  of  hercredit records, eliminating the risk of privacy violations or databreaches. Moreover, our approach provides strong guaranteesfor the lenders as well. A lender can check both correctness andcompleteness of the credit data disclosed to her. This is the firstapproach  that  can  perform  all  credit  reporting  tasks  withouta  central  authority  or  changing  the  financial  mechanisms*.","lang":"eng"}],"arxiv":1,"page":"1343-1348","conference":{"end_date":"2018-08-03","location":"Halifax, Canada","start_date":"2018-07-30","name":"IEEE International Conference on Blockchain"},"date_updated":"2026-10-05T22:30:59Z","file":[{"date_created":"2019-04-18T10:36:39Z","file_size":624338,"checksum":"b25c9bb7cf6e7e6634e692d26d41ead8","content_type":"application/pdf","file_id":"6341","creator":"akafshda","access_level":"open_access","file_name":"blockchain2018.pdf","date_updated":"2020-07-14T12:47:27Z","relation":"main_file"}],"department":[{"_id":"KrCh"}],"_id":"6340","title":"Secure Credit Reporting on the Blockchain","publication_identifier":{"isbn":["978-1-5386-7975-3 "]},"date_created":"2019-04-18T10:37:35Z","fulldoi":"https://doi.org/10.1109/Cybermatics_2018.2018.00231","oa_version":"Submitted Version","publication_status":"published","article_processing_charge":"No","oa":1},{"publist_id":"7554","publisher":"Springer","type":"conference","project":[{"name":"Efficient Algorithms for Computer Aided Verification","_id":"25892FC0-B435-11E9-9278-68D0E5697425","grant_number":"ICT15-003"},{"call_identifier":"FWF","_id":"25832EC2-B435-11E9-9278-68D0E5697425","name":"Rigorous Systems Engineering","grant_number":"S 11407_N23"},{"grant_number":"279307","call_identifier":"FP7","_id":"2581B60A-B435-11E9-9278-68D0E5697425","name":"Quantitative Graph Games: Theory and Applications"}],"year":"2018","language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"Smart contracts are computer programs that are executed by a network of mutually distrusting agents, without the need of an external trusted authority. Smart contracts handle and transfer assets of considerable value (in the form of crypto-currency like Bitcoin). Hence, it is crucial that their implementation is bug-free. We identify the utility (or expected payoff) of interacting with such smart contracts as the basic and canonical quantitative property for such contracts. We present a framework for such quantitative analysis of smart contracts. Such a formal framework poses new and novel research challenges in programming languages, as it requires modeling of game-theoretic aspects to analyze incentives for deviation from honest behavior and modeling utilities which are not specified as standard temporal properties such as safety and termination. While game-theoretic incentives have been analyzed in the security community, their analysis has been restricted to the very special case of stateless games. However, to analyze smart contracts, stateful analysis is required as it must account for the different program states of the protocol. Our main contributions are as follows: we present (i)~a simplified programming language for smart contracts; (ii)~an automatic translation of the programs to state-based games; (iii)~an abstraction-refinement approach to solve such games; and (iv)~experimental results on real-world-inspired smart contracts."}],"volume":10801,"page":"739 - 767","intvolume":"     10801","date_updated":"2026-10-05T22:30:58Z","conference":{"name":"ESOP: European Symposium on Programming","start_date":"2018-04-16","location":"Thessaloniki, Greece","end_date":"2018-04-19"},"file":[{"date_created":"2018-12-17T15:45:49Z","file_size":1394993,"date_updated":"2020-07-14T12:46:00Z","relation":"main_file","access_level":"open_access","file_name":"2018_ESOP_Chatterjee.pdf","file_id":"5716","content_type":"application/pdf","checksum":"9c8a8338c571903b599b6ca93abd2cce","creator":"dernst"}],"department":[{"_id":"KrCh"}],"title":"Quantitative analysis of smart contracts","_id":"311","date_created":"2018-12-11T11:45:45Z","oa_version":"Published Version","fulldoi":"https://doi.org/10.1007/978-3-319-89884-1_26","publication_status":"published","article_processing_charge":"No","oa":1,"date_published":"2018-04-01T00:00:00Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"file_date_updated":"2020-07-14T12:46:00Z","has_accepted_license":"1","acknowledgement":"The research was partially supported by Vienna Science and Technology Fund (WWTF) Project ICT15-003, Austrian Science Fund (FWF) NFN Grant No S11407-N23 (RiSE/SHiNE), and ERC Starting grant (279307: Graph Games).","citation":{"ama":"Chatterjee K, Goharshady AK, Velner Y. Quantitative analysis of smart contracts. In: Vol 10801. Springer; 2018:739-767. doi:<a href=\"https://doi.org/10.1007/978-3-319-89884-1_26\">10.1007/978-3-319-89884-1_26</a>","ieee":"K. Chatterjee, A. K. Goharshady, and Y. Velner, “Quantitative analysis of smart contracts,” presented at the ESOP: European Symposium on Programming, Thessaloniki, Greece, 2018, vol. 10801, pp. 739–767.","short":"K. Chatterjee, A.K. Goharshady, Y. Velner, in:, Springer, 2018, pp. 739–767.","ista":"Chatterjee K, Goharshady AK, Velner Y. 2018. Quantitative analysis of smart contracts. ESOP: European Symposium on Programming, LNCS, vol. 10801, 739–767.","apa":"Chatterjee, K., Goharshady, A. K., &#38; Velner, Y. (2018). Quantitative analysis of smart contracts (Vol. 10801, pp. 739–767). Presented at the ESOP: European Symposium on Programming, Thessaloniki, Greece: Springer. <a href=\"https://doi.org/10.1007/978-3-319-89884-1_26\">https://doi.org/10.1007/978-3-319-89884-1_26</a>","mla":"Chatterjee, Krishnendu, et al. <i>Quantitative Analysis of Smart Contracts</i>. Vol. 10801, Springer, 2018, pp. 739–67, doi:<a href=\"https://doi.org/10.1007/978-3-319-89884-1_26\">10.1007/978-3-319-89884-1_26</a>.","chicago":"Chatterjee, Krishnendu, Amir Kafshdar Goharshady, and Yaron Velner. “Quantitative Analysis of Smart Contracts,” 10801:739–67. Springer, 2018. <a href=\"https://doi.org/10.1007/978-3-319-89884-1_26\">https://doi.org/10.1007/978-3-319-89884-1_26</a>."},"ec_funded":1,"scopus_import":"1","quality_controlled":"1","related_material":{"record":[{"id":"8934","relation":"dissertation_contains","status":"public"}]},"ddc":["000"],"status":"public","month":"04","author":[{"last_name":"Chatterjee","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","full_name":"Chatterjee, Krishnendu","orcid":"0000-0002-4561-241X","first_name":"Krishnendu"},{"last_name":"Goharshady","id":"391365CE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1702-6584","full_name":"Goharshady, Amir","first_name":"Amir"},{"last_name":"Velner","full_name":"Velner, Yaron","first_name":"Yaron"}],"day":"01","alternative_title":["LNCS"],"doi":"10.1007/978-3-319-89884-1_26","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"date_published":"2018-08-01T00:00:00Z","corr_author":"1","external_id":{"isi":["000444694800001"],"arxiv":["1510.07565"]},"ec_funded":1,"scopus_import":"1","citation":{"ieee":"K. Chatterjee, R. Ibsen-Jensen, A. K. Goharshady, and A. Pavlogiannis, “Algorithms for algebraic path properties in concurrent systems of constant treewidth components,” <i>ACM Transactions on Programming Languages and Systems</i>, vol. 40, no. 3. Association for Computing Machinery, 2018.","ama":"Chatterjee K, Ibsen-Jensen R, Goharshady AK, Pavlogiannis A. Algorithms for algebraic path properties in concurrent systems of constant treewidth components. <i>ACM Transactions on Programming Languages and Systems</i>. 2018;40(3). doi:<a href=\"https://doi.org/10.1145/3210257\">10.1145/3210257</a>","chicago":"Chatterjee, Krishnendu, Rasmus Ibsen-Jensen, Amir Kafshdar Goharshady, and Andreas Pavlogiannis. “Algorithms for Algebraic Path Properties in Concurrent Systems of Constant Treewidth Components.” <i>ACM Transactions on Programming Languages and Systems</i>. Association for Computing Machinery, 2018. <a href=\"https://doi.org/10.1145/3210257\">https://doi.org/10.1145/3210257</a>.","ista":"Chatterjee K, Ibsen-Jensen R, Goharshady AK, Pavlogiannis A. 2018. Algorithms for algebraic path properties in concurrent systems of constant treewidth components. ACM Transactions on Programming Languages and Systems. 40(3), 9.","apa":"Chatterjee, K., Ibsen-Jensen, R., Goharshady, A. K., &#38; Pavlogiannis, A. (2018). Algorithms for algebraic path properties in concurrent systems of constant treewidth components. <i>ACM Transactions on Programming Languages and Systems</i>. Association for Computing Machinery. <a href=\"https://doi.org/10.1145/3210257\">https://doi.org/10.1145/3210257</a>","short":"K. Chatterjee, R. Ibsen-Jensen, A.K. Goharshady, A. Pavlogiannis, ACM Transactions on Programming Languages and Systems 40 (2018).","mla":"Chatterjee, Krishnendu, et al. “Algorithms for Algebraic Path Properties in Concurrent Systems of Constant Treewidth Components.” <i>ACM Transactions on Programming Languages and Systems</i>, vol. 40, no. 3, 9, Association for Computing Machinery, 2018, doi:<a href=\"https://doi.org/10.1145/3210257\">10.1145/3210257</a>."},"isi":1,"status":"public","month":"08","author":[{"orcid":"0000-0002-4561-241X","full_name":"Chatterjee, Krishnendu","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","first_name":"Krishnendu","last_name":"Chatterjee"},{"first_name":"Rasmus","id":"3B699956-F248-11E8-B48F-1D18A9856A87","full_name":"Ibsen-Jensen, Rasmus","orcid":"0000-0003-4783-0389","last_name":"Ibsen-Jensen"},{"first_name":"Amir Kafshdar","full_name":"Goharshady, Amir Kafshdar","orcid":"0000-0003-1702-6584","id":"391365CE-F248-11E8-B48F-1D18A9856A87","last_name":"Goharshady"},{"first_name":"Andreas","orcid":"0000-0002-8943-0722","full_name":"Pavlogiannis, Andreas","id":"49704004-F248-11E8-B48F-1D18A9856A87","last_name":"Pavlogiannis"}],"main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1510.07565"}],"issue":"3","publication":"ACM Transactions on Programming Languages and Systems","quality_controlled":"1","related_material":{"record":[{"status":"public","relation":"earlier_version","id":"5441"},{"id":"5442","status":"public","relation":"earlier_version"},{"relation":"earlier_version","status":"public","id":"1437"},{"id":"8934","relation":"dissertation_contains","status":"public"}]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1145/3210257","day":"01","publisher":"Association for Computing Machinery","type":"journal_article","project":[{"grant_number":"P 23499-N23","call_identifier":"FWF","_id":"2584A770-B435-11E9-9278-68D0E5697425","name":"Modern Graph Algorithmic Techniques in Formal Verification"},{"_id":"25832EC2-B435-11E9-9278-68D0E5697425","name":"Rigorous Systems Engineering","call_identifier":"FWF","grant_number":"S 11407_N23"},{"grant_number":"279307","name":"Quantitative Graph Games: Theory and Applications","_id":"2581B60A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"year":"2018","language":[{"iso":"eng"}],"intvolume":"        40","date_updated":"2026-10-05T22:30:59Z","arxiv":1,"abstract":[{"text":"We study algorithmic questions wrt algebraic path properties in concurrent systems, where the transitions of the system are labeled from a complete, closed semiring. The algebraic path properties can model dataflow analysis problems, the shortest path problem, and many other natural problems that arise in program analysis. We consider that each component of the concurrent system is a graph with constant treewidth, a property satisfied by the controlflow graphs of most programs. We allow for multiple possible queries, which arise naturally in demand driven dataflow analysis. The study of multiple queries allows us to consider the tradeoff between the resource usage of the one-time preprocessing and for each individual query. The traditional approach constructs the product graph of all components and applies the best-known graph algorithm on the product. In this approach, even the answer to a single query requires the transitive closure (i.e., the results of all possible queries), which provides no room for tradeoff between preprocessing and query time.\r\nOur main contributions are algorithms that significantly improve the worst-case running time of the traditional approach, and provide various tradeoffs depending on the number of queries. For example, in a concurrent system of two components, the traditional approach requires hexic time in the worst case for answering one query as well as computing the transitive closure, whereas we show that with one-time preprocessing in almost cubic time, each subsequent query can be answered in at most linear time, and even the transitive closure can be computed in almost quartic time. Furthermore, we establish conditional optimality results showing that the worst-case running time of our algorithms cannot be improved without achieving major breakthroughs in graph algorithms (i.e., improving the worst-case bound for the shortest path problem in general graphs). Preliminary experimental results show that our algorithms perform favorably on several benchmarks.\r\n","lang":"eng"}],"volume":40,"article_number":"9","publication_identifier":{"issn":["0164-0925"]},"date_created":"2019-02-14T14:31:52Z","department":[{"_id":"KrCh"}],"title":"Algorithms for algebraic path properties in concurrent systems of constant treewidth components","_id":"6009","article_processing_charge":"No","oa":1,"oa_version":"Preprint","fulldoi":"https://doi.org/10.1145/3210257","publication_status":"published"},{"intvolume":"      2018","date_updated":"2026-10-05T22:30:59Z","conference":{"end_date":"2018-07-19","location":"Stockholm, Sweden","start_date":"2018-07-13","name":"IJCAI: International Joint Conference on Artificial Intelligence"},"arxiv":1,"abstract":[{"text":"We consider the stochastic shortest path (SSP)problem for succinct Markov decision processes(MDPs), where the MDP consists of a set of vari-ables, and a set of nondeterministic rules that up-date the variables. First, we show that several ex-amples from the AI literature can be modeled assuccinct MDPs.  Then we present computationalapproaches for upper and lower bounds for theSSP problem: (a) for computing upper bounds, ourmethod is polynomial-time in the implicit descrip-tion of the MDP; (b) for lower bounds, we present apolynomial-time (in the size of the implicit descrip-tion) reduction to quadratic programming. Our ap-proach is applicable even to infinite-state MDPs.Finally, we present experimental results to demon-strate the effectiveness of our approach on severalclassical examples from the AI literature.","lang":"eng"}],"volume":2018,"page":"4700-4707","publisher":"IJCAI","type":"conference","project":[{"_id":"25892FC0-B435-11E9-9278-68D0E5697425","name":"Efficient Algorithms for Computer Aided Verification","grant_number":"ICT15-003"},{"call_identifier":"FWF","name":"Rigorous Systems Engineering","_id":"25832EC2-B435-11E9-9278-68D0E5697425","grant_number":"S 11407_N23"},{"grant_number":"279307","name":"Quantitative Graph Games: Theory and Applications","_id":"2581B60A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"year":"2018","language":[{"iso":"eng"}],"article_processing_charge":"No","oa":1,"oa_version":"Preprint","fulldoi":"https://doi.org/10.24963/ijcai.2018/653","publication_status":"published","publication_identifier":{"issn":["1045-0823"],"isbn":["9780999241127"]},"date_created":"2019-02-13T13:26:27Z","department":[{"_id":"KrCh"}],"title":"Computational approaches for stochastic shortest path on succinct MDPs","_id":"5977","ec_funded":1,"scopus_import":"1","citation":{"ama":"Chatterjee K, Fu H, Goharshady AK, Okati N. Computational approaches for stochastic shortest path on succinct MDPs. In: <i>Proceedings of the Twenty-Seventh International Joint Conference on Artificial Intelligence</i>. Vol 2018. IJCAI; 2018:4700-4707. doi:<a href=\"https://doi.org/10.24963/ijcai.2018/653\">10.24963/ijcai.2018/653</a>","ieee":"K. Chatterjee, H. Fu, A. K. Goharshady, and N. Okati, “Computational approaches for stochastic shortest path on succinct MDPs,” in <i>Proceedings of the Twenty-Seventh International Joint Conference on Artificial Intelligence</i>, Stockholm, Sweden, 2018, vol. 2018, pp. 4700–4707.","chicago":"Chatterjee, Krishnendu, Hongfei Fu, Amir Kafshdar Goharshady, and Nastaran Okati. “Computational Approaches for Stochastic Shortest Path on Succinct MDPs.” In <i>Proceedings of the Twenty-Seventh International Joint Conference on Artificial Intelligence</i>, 2018:4700–4707. IJCAI, 2018. <a href=\"https://doi.org/10.24963/ijcai.2018/653\">https://doi.org/10.24963/ijcai.2018/653</a>.","mla":"Chatterjee, Krishnendu, et al. “Computational Approaches for Stochastic Shortest Path on Succinct MDPs.” <i>Proceedings of the Twenty-Seventh International Joint Conference on Artificial Intelligence</i>, vol. 2018, IJCAI, 2018, pp. 4700–07, doi:<a href=\"https://doi.org/10.24963/ijcai.2018/653\">10.24963/ijcai.2018/653</a>.","apa":"Chatterjee, K., Fu, H., Goharshady, A. K., &#38; Okati, N. (2018). Computational approaches for stochastic shortest path on succinct MDPs. In <i>Proceedings of the Twenty-Seventh International Joint Conference on Artificial Intelligence</i> (Vol. 2018, pp. 4700–4707). Stockholm, Sweden: IJCAI. <a href=\"https://doi.org/10.24963/ijcai.2018/653\">https://doi.org/10.24963/ijcai.2018/653</a>","ista":"Chatterjee K, Fu H, Goharshady AK, Okati N. 2018. Computational approaches for stochastic shortest path on succinct MDPs. Proceedings of the Twenty-Seventh International Joint Conference on Artificial Intelligence. IJCAI: International Joint Conference on Artificial Intelligence vol. 2018, 4700–4707.","short":"K. Chatterjee, H. Fu, A.K. Goharshady, N. Okati, in:, Proceedings of the Twenty-Seventh International Joint Conference on Artificial Intelligence, IJCAI, 2018, pp. 4700–4707."},"date_published":"2018-07-17T00:00:00Z","external_id":{"arxiv":["1804.08984"],"isi":["000764175404118"]},"doi":"10.24963/ijcai.2018/653","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"17","status":"public","isi":1,"month":"07","author":[{"last_name":"Chatterjee","first_name":"Krishnendu","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","full_name":"Chatterjee, Krishnendu","orcid":"0000-0002-4561-241X"},{"last_name":"Fu","first_name":"Hongfei","full_name":"Fu, Hongfei","id":"3AAD03D6-F248-11E8-B48F-1D18A9856A87"},{"id":"391365CE-F248-11E8-B48F-1D18A9856A87","full_name":"Goharshady, Amir","orcid":"0000-0003-1702-6584","first_name":"Amir","last_name":"Goharshady"},{"first_name":"Nastaran","full_name":"Okati, Nastaran","last_name":"Okati"}],"main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1804.08984"}],"publication":"Proceedings of the Twenty-Seventh International Joint Conference on Artificial Intelligence","quality_controlled":"1","related_material":{"record":[{"id":"8934","status":"public","relation":"dissertation_contains"}]}},{"OA_place":"publisher","publication_status":"published","fulldoi":"https://doi.org/10.15479/AT:ISTA:th1072","oa_version":"Published Version","oa":1,"article_processing_charge":"No","_id":"6263","title":"Genetic determinants of antibiotic resistance evolution","degree_awarded":"PhD","department":[{"_id":"ToBo"},{"_id":"GradSch"}],"date_created":"2019-04-09T13:57:15Z","publication_identifier":{"issn":["2663-337X"]},"page":"91","abstract":[{"lang":"eng","text":"Antibiotic  resistance  can  emerge  spontaneously  through  genomic  mutation  and  render treatment   ineffective.   To   counteract   this process, in   addition   to   the   discovery   and description of resistance mechanisms,a deeper understanding of resistanceevolvabilityand its  determinantsis  needed. To address  this challenge,  this  thesisuncoversnew  genetic determinants   of   resistance   evolvability   using   a   customized   robotic   setup, exploressystematic   ways   in   which   resistance   evolution   is   perturbed   due   to dose-responsecharacteristics  of  drugs and  mutation  rate  differences,and  mathematically  investigates the evolutionary fate of one specific type of evolvability modifier -a stress-induced mutagenesis allele.We  find  severalgenes  which  strongly  inhibit  or  potentiate  resistance  evolution.  In  order to identify   them,   we   first developedan   automated   high-throughput   feedback-controlled protocol whichkeeps the population size and selection pressure approximately constant for hundreds  of  cultures  by  dynamically  re-diluting  the  cultures  and  adjusting  the  antibiotic concentration.  We  implementedthis  protocol  on  a  customized  liquid  handling  robot  and propagated  100  different  gene  deletion  strains  of Escherichia  coliin  triplicate  for  over  100 generations  in  tetracycline  and  in  chloramphenicol,  and  comparedtheir  adaptation  rates.We  find  a  diminishing  returns  pattern,  where  initially  sensitive  strains  adapted  more compared to less sensitive ones.  Our data uncover that deletions of certain genes which do not  affect  mutation  rate,including  efflux  pump  components,  a  chaperone  and severalstructural  and regulatory  genes  can strongly  and  reproducibly  alterresistance  evolution. Sequencing   analysis of   evolved   populations   indicates   that   epistasis   with   resistance mutations  is  the  most  likelyexplanation. This  work  could  inspire  treatment  strategies  in which  targeted  inhibitors  of  evolvability  mechanisms  will  be  given  alongside  antibiotics  to slow down resistance evolution and extend theefficacy of antibiotics.We implemented  astochasticpopulation  genetics  model, toverifyways  in  which  general properties,  namely,  dose-response  characteristics  of  drugs  and  mutation  rates,  influence evolutionary  dynamics.  In  particular,  under  the  exposure  to  antibiotics  with  shallow  dose-response  curves,bacteria  have  narrower  distributions  of  fitness  effects  of  new  mutations. We  show  that in  silicothis  also  leads  to  slower  resistance  evolution.  We see and  confirm with experiments that increased mutation rates, apart from speeding up evolution, also leadto high reproducibility of phenotypic adaptation in a context of continually strong selection pressure.Knowledge  of  these  patterns  can  aid  in  predicting  the  dynamics  of  antibiotic resistance evolutionand adapting treatment schemes accordingly.Focusing on   a   previously   described   type   of   evolvability   modifier –a   stress-induced mutagenesis  allele –we  find  conditions  under  which  it  can  persist  in  a  population  under periodic  selectionakin  to  clinical  treatment. We  set  up  a  deterministic infinite  populationcontinuous  time  model  tracking  the  frequencies  of  a  mutator  and  resistance  allele  and evaluate  various  treatment  schemes  in  how  well  they  maintain  a stress-induced mutator allele. In particular,a high diversity  of stresses  is  crucial  for  the  persistence of the  mutator allele. This leads to a general trade-off where exactly those diversifying treatment schemes which  are  likely  to  decrease  levels  of  resistance  could  lead  to  stronger  selection  of  highly evolvable genotypes.In  the  long  run,  this  work  will  lead  to  a  deeper  understanding  of  the  genetic  and  cellular mechanisms involved in antibiotic resistance evolution and could inspire new strategies for slowing down its rate. "}],"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"LifeSc"}],"file":[{"file_size":5656866,"date_created":"2019-04-09T13:49:24Z","embargo":"2020-01-25","file_name":"2018_Thesis_Lukacisinova.pdf","access_level":"open_access","relation":"main_file","date_updated":"2021-02-11T11:17:17Z","creator":"dernst","checksum":"fc60585c9eaad868ac007004ef130908","content_type":"application/pdf","file_id":"6264"},{"date_created":"2019-04-09T13:49:23Z","file_size":5168054,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"264057ec0a92ab348cc83b41f021ba92","file_id":"6265","embargo_to":"open_access","creator":"dernst","file_name":"2018_Thesis_Lukacisinova_source.docx","access_level":"closed","relation":"source_file","date_updated":"2020-07-14T12:47:25Z"}],"date_updated":"2026-07-30T14:47:59Z","doi_confirm":"1","language":[{"iso":"eng"}],"year":"2018","type":"dissertation","publisher":"Institute of Science and Technology Austria","supervisor":[{"last_name":"Bollenbach","first_name":"Tobias","orcid":"0000-0003-4398-476X","full_name":"Bollenbach, Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87"}],"day":"28","doi":"10.15479/AT:ISTA:th1072","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","alternative_title":["ISTA Thesis"],"related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"1027"},{"relation":"part_of_dissertation","status":"public","id":"696"},{"id":"1619","relation":"part_of_dissertation","status":"public"}]},"author":[{"first_name":"Marta","id":"4342E402-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2519-8004","full_name":"Lukacisinova, Marta","last_name":"Lukacisinova"}],"ddc":["570","576","579"],"status":"public","month":"12","citation":{"ista":"Lukacisinova M. 2018. Genetic determinants of antibiotic resistance evolution. Institute of Science and Technology Austria.","short":"M. Lukacisinova, Genetic Determinants of Antibiotic Resistance Evolution, Institute of Science and Technology Austria, 2018.","apa":"Lukacisinova, M. (2018). <i>Genetic determinants of antibiotic resistance evolution</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th1072\">https://doi.org/10.15479/AT:ISTA:th1072</a>","mla":"Lukacisinova, Marta. <i>Genetic Determinants of Antibiotic Resistance Evolution</i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th1072\">10.15479/AT:ISTA:th1072</a>.","chicago":"Lukacisinova, Marta. “Genetic Determinants of Antibiotic Resistance Evolution.” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:th1072\">https://doi.org/10.15479/AT:ISTA:th1072</a>.","ieee":"M. Lukacisinova, “Genetic determinants of antibiotic resistance evolution,” Institute of Science and Technology Austria, 2018.","ama":"Lukacisinova M. Genetic determinants of antibiotic resistance evolution. 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th1072\">10.15479/AT:ISTA:th1072</a>"},"corr_author":"1","has_accepted_license":"1","file_date_updated":"2021-02-11T11:17:17Z","date_published":"2018-12-28T00:00:00Z"},{"day":"01","article_type":"original","doi":"10.1007/s00429-017-1568-y","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication":"Brain Structure and Function","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"9562"}]},"quality_controlled":"1","ddc":["571"],"isi":1,"status":"public","month":"04","author":[{"last_name":"Luján","full_name":"Luján, Rafael","first_name":"Rafael"},{"last_name":"Aguado","first_name":"Carolina","full_name":"Aguado, Carolina"},{"full_name":"Ciruela, Francisco","first_name":"Francisco","last_name":"Ciruela"},{"last_name":"Cózar","full_name":"Cózar, Javier","first_name":"Javier"},{"first_name":"David","full_name":"Kleindienst, David","id":"42E121A4-F248-11E8-B48F-1D18A9856A87","last_name":"Kleindienst"},{"last_name":"De La Ossa","first_name":"Luis","full_name":"De La Ossa, Luis"},{"full_name":"Bettler, Bernhard","first_name":"Bernhard","last_name":"Bettler"},{"full_name":"Wickman, Kevin","first_name":"Kevin","last_name":"Wickman"},{"last_name":"Watanabe","full_name":"Watanabe, Masahiko","first_name":"Masahiko"},{"last_name":"Shigemoto","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444"},{"last_name":"Fukazawa","first_name":"Yugo","full_name":"Fukazawa, Yugo"}],"issue":"3","citation":{"apa":"Luján, R., Aguado, C., Ciruela, F., Cózar, J., Kleindienst, D., De La Ossa, L., … Fukazawa, Y. (2018). Differential association of GABAB receptors with their effector ion channels in Purkinje cells. <i>Brain Structure and Function</i>. Springer. <a href=\"https://doi.org/10.1007/s00429-017-1568-y\">https://doi.org/10.1007/s00429-017-1568-y</a>","short":"R. Luján, C. Aguado, F. Ciruela, J. Cózar, D. Kleindienst, L. De La Ossa, B. Bettler, K. Wickman, M. Watanabe, R. Shigemoto, Y. Fukazawa, Brain Structure and Function 223 (2018) 1565–1587.","ista":"Luján R, Aguado C, Ciruela F, Cózar J, Kleindienst D, De La Ossa L, Bettler B, Wickman K, Watanabe M, Shigemoto R, Fukazawa Y. 2018. Differential association of GABAB receptors with their effector ion channels in Purkinje cells. Brain Structure and Function. 223(3), 1565–1587.","mla":"Luján, Rafael, et al. “Differential Association of GABAB Receptors with Their Effector Ion Channels in Purkinje Cells.” <i>Brain Structure and Function</i>, vol. 223, no. 3, Springer, 2018, pp. 1565–87, doi:<a href=\"https://doi.org/10.1007/s00429-017-1568-y\">10.1007/s00429-017-1568-y</a>.","chicago":"Luján, Rafael, Carolina Aguado, Francisco Ciruela, Javier Cózar, David Kleindienst, Luis De La Ossa, Bernhard Bettler, et al. “Differential Association of GABAB Receptors with Their Effector Ion Channels in Purkinje Cells.” <i>Brain Structure and Function</i>. Springer, 2018. <a href=\"https://doi.org/10.1007/s00429-017-1568-y\">https://doi.org/10.1007/s00429-017-1568-y</a>.","ama":"Luján R, Aguado C, Ciruela F, et al. Differential association of GABAB receptors with their effector ion channels in Purkinje cells. <i>Brain Structure and Function</i>. 2018;223(3):1565-1587. doi:<a href=\"https://doi.org/10.1007/s00429-017-1568-y\">10.1007/s00429-017-1568-y</a>","ieee":"R. Luján <i>et al.</i>, “Differential association of GABAB receptors with their effector ion channels in Purkinje cells,” <i>Brain Structure and Function</i>, vol. 223, no. 3. Springer, pp. 1565–1587, 2018."},"ec_funded":1,"scopus_import":"1","external_id":{"isi":["000428419500030"]},"date_published":"2018-04-01T00:00:00Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"has_accepted_license":"1","file_date_updated":"2020-07-14T12:47:20Z","oa_version":"Published Version","fulldoi":"https://doi.org/10.1007/s00429-017-1568-y","publication_status":"published","article_processing_charge":"No","oa":1,"department":[{"_id":"RySh"}],"title":"Differential association of GABAB receptors with their effector ion channels in Purkinje cells","_id":"612","date_created":"2018-12-11T11:47:29Z","abstract":[{"lang":"eng","text":"Metabotropic GABAB receptors mediate slow inhibitory effects presynaptically and postsynaptically through the modulation of different effector signalling pathways. Here, we analysed the distribution of GABAB receptors using highly sensitive SDS-digested freeze-fracture replica labelling in mouse cerebellar Purkinje cells. Immunoreactivity for GABAB1 was observed on presynaptic and, more abundantly, on postsynaptic compartments, showing both scattered and clustered distribution patterns. Quantitative analysis of immunoparticles revealed a somato-dendritic gradient, with the density of immunoparticles increasing 26-fold from somata to dendritic spines. To understand the spatial relationship of GABAB receptors with two key effector ion channels, the G protein-gated inwardly rectifying K+ (GIRK/Kir3) channel and the voltage-dependent Ca2+ channel, biochemical and immunohistochemical approaches were performed. Co-immunoprecipitation analysis demonstrated that GABAB receptors co-assembled with GIRK and CaV2.1 channels in the cerebellum. Using double-labelling immunoelectron microscopic techniques, co-clustering between GABAB1 and GIRK2 was detected in dendritic spines, whereas they were mainly segregated in the dendritic shafts. In contrast, co-clustering of GABAB1 and CaV2.1 was detected in dendritic shafts but not spines. Presynaptically, although no significant co-clustering of GABAB1 and GIRK2 or CaV2.1 channels was detected, inter-cluster distance for GABAB1 and GIRK2 was significantly smaller in the active zone than in the dendritic shafts, and that for GABAB1 and CaV2.1 was significantly smaller in the active zone than in the dendritic shafts and spines. Thus, GABAB receptors are associated with GIRK and CaV2.1 channels in different subcellular compartments. These data provide a better framework for understanding the different roles played by GABAB receptors and their effector ion channels in the cerebellar network."}],"pubrep_id":"1013","volume":223,"page":"1565 - 1587","date_updated":"2026-10-05T22:31:03Z","intvolume":"       223","file":[{"file_size":5542926,"date_created":"2018-12-12T10:15:36Z","access_level":"open_access","file_name":"IST-2018-1013-v1+1_2018_Kleindienst_Differential.pdf","relation":"main_file","date_updated":"2020-07-14T12:47:20Z","creator":"system","content_type":"application/pdf","checksum":"a55b3103476ecb5f4f983d8801807e8b","file_id":"5157"}],"publist_id":"7192","publisher":"Springer","type":"journal_article","year":"2018","project":[{"grant_number":"720270","name":"Human Brain Project Specific Grant Agreement 1","_id":"25CBA828-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"291734","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"language":[{"iso":"eng"}]}]