[{"publication_status":"published","department":[{"_id":"GradSch"},{"_id":"SaSi"}],"abstract":[{"text":"Prenatal immune challenges pose significant risks to human embryonic brain and eye development. However, we still lack knowledge about the safe usage of anti-inflammatory drugs during pregnancy. Human induced pluripotent stem cell (hIPSC)-derived brain organoid models provide a unique opportunity to investigate neuronal development and have started to explore functional consequences upon viral infection. However, brain organoids usually lack microglia, the brain-resident immune cells. They are present in the early human embryonic brain and actively participate in neuronal circuit development. At the same time, microglia are known for their immune-sensing properties and will influence viral-mediated effects. In my thesis, I was interested to study the multifunctional role of human microglia during retinal development. \r\nIn chapter 1, I characterize the innate occurrence of IBA1+-microglia-like cells within the retinal organoid differentiation (Bartalska et al., 2022). Therefore, we differentiate hIPSC using an unguided retinal organoid differentiation protocol and observe the presence of IBA1+-microglia-like cells alongside retinal cups between week 3 and 4 in 2.5D culture. However, instead of infiltrating the neuroectodermal sides, they enrich within non-pigmented, 3D-cystic compartments that develop in low numbers parallel to 3D-retinal organoids. To enrich for IBA1+-microglia precursors (preMG), we guided the differentiation with a low-dosed BMP4 application, which prevents retinal cup development and enhances microglia and 3D-cysts formation. We characterize the differentiated preMG for their microglia-like identity and validated their functionality. In parallel, mass spectrometry identifies the 3D-cysts to express mesenchymal and epithelial markers. We confirm that comparable 3D-cysts are also the preferential environment for IBA1+-microglia-like cells within the unguided retinal organoid differentiation. \r\nIn chapter 2, I investigate how microglia influence retinal development and whether they contribute to viral-mediated consequences (Schmied et al., 2025). Here, we assemble preMG, which we have characterized in chapter 1, into 3D-retinal organoids. Once the outer plexiform layer forms, microglia-like cells (iMG) populate them and interact with retinal cell types. However, at this developmental stage, the ganglion cell number decreases in 3D-retinal organoids. Thus, we adapted the model into 2D which promotes their survival. Integrated iMG engulf ganglion cells and control their cell number. In parallel, we apply the immunostimulant POLY(I:C) to mimic a fetal viral infection. Although POLY(I:C) stimulation affects iMG phenotype, it does not influence their interaction with ganglion cells. Furthermore, iMG presence significantly contributes to the supernatant’s inflammatory secretome and increases retinal cell proliferation. Simultaneous exposure to the non-steroidal anti-inflammatory drug (NSAID) ibuprofen dampens POLY(I:C)-mediated consequences of the iMG phenotype and ameliorates cell proliferation. Remarkably, while POLY(I:C) disrupts neuronal calcium dynamics independent of iMG presence, ibuprofen rescues this effect only in the presence of iMG. Mechanistically, ibuprofen blocks the enzymes cyclooxygenase 1 and 2 (COX1/ PTGS1 and COX2/ PTGS2) simultaneously, from which iMG predominantly express COX1. Selective inhibition of COX1 does not restore the calcium peak amplitude upon POLY(I:C) stimulation, indicating ibuprofen’s effect depends on the presence and interplay of both, COX1 and COX2. \r\nIn summary, we characterized the 3D-retinal organoid model for the occurrence of IBA1+-microglia like cells. As the innately developing IBA1+-cells enrich in mesenchymal over retinal structures, we optimized a protocol to differentiate IBA1+-microglia precursors. By combining these two models we generate microglia-assembled retinal organoids. Our results underscore the importance of microglia during neurodevelopment, in the context of prenatal immune challenges and provide insight into the mechanisms by which ibuprofen exerts its protective effects during embryonic development.","lang":"eng"}],"doi":"10.15479/AT-ISTA-20074","degree_awarded":"PhD","alternative_title":["ISTA Thesis"],"file_date_updated":"2025-07-30T09:29:09Z","file":[{"content_type":"application/x-zip-compressed","date_created":"2025-07-30T08:47:53Z","relation":"source_file","checksum":"d09f9984002353ad7442358394919bf3","file_id":"20086","file_size":43566093,"file_name":"PhD_Thesis_Schmied.zip","date_updated":"2025-07-30T08:47:53Z","access_level":"closed","creator":"vhuebsch"},{"date_updated":"2025-07-30T09:29:09Z","file_size":13120922,"file_name":"PhD_Thesis_Schmied.pdf","access_level":"open_access","creator":"vhuebsch","content_type":"application/pdf","date_created":"2025-07-30T08:47:46Z","relation":"main_file","checksum":"4833690d7283c587f518ba98eeb2c946","file_id":"20087"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","year":"2025","date_created":"2025-07-24T12:37:22Z","date_published":"2025-07-24T00:00:00Z","project":[{"name":"How human microglia shape developing neurons during health and inflammation","grant_number":"SC19-017","_id":"9B99D380-BA93-11EA-9121-9846C619BF3A"}],"ddc":["570"],"date_updated":"2026-05-06T12:49:36Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"title":" Human microglia impact neuronal development in retinal organoids","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"11478"},{"relation":"part_of_dissertation","status":"public","id":"19593"}]},"oa_version":"Published Version","citation":{"chicago":"Schmied, Verena. “ Human Microglia Impact Neuronal Development in Retinal Organoids.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20074\">https://doi.org/10.15479/AT-ISTA-20074</a>.","mla":"Schmied, Verena. <i> Human Microglia Impact Neuronal Development in Retinal Organoids</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20074\">10.15479/AT-ISTA-20074</a>.","apa":"Schmied, V. (2025). <i> Human microglia impact neuronal development in retinal organoids</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20074\">https://doi.org/10.15479/AT-ISTA-20074</a>","ista":"Schmied V. 2025.  Human microglia impact neuronal development in retinal organoids. Institute of Science and Technology Austria.","short":"V. Schmied,  Human Microglia Impact Neuronal Development in Retinal Organoids, Institute of Science and Technology Austria, 2025.","ama":"Schmied V.  Human microglia impact neuronal development in retinal organoids. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20074\">10.15479/AT-ISTA-20074</a>","ieee":"V. Schmied, “ Human microglia impact neuronal development in retinal organoids,” Institute of Science and Technology Austria, 2025."},"has_accepted_license":"1","day":"24","type":"dissertation","OA_place":"publisher","status":"public","page":"151","month":"07","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-060-2"]},"publisher":"Institute of Science and Technology Austria","article_processing_charge":"No","corr_author":"1","_id":"20074","oa":1,"language":[{"iso":"eng"}],"author":[{"first_name":"Verena","full_name":"Hübschmann, Verena","last_name":"Hübschmann","id":"32B7C918-F248-11E8-B48F-1D18A9856A87"}],"supervisor":[{"full_name":"Siegert, Sandra","orcid":"0000-0001-8635-0877","last_name":"Siegert","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","first_name":"Sandra"}]},{"title":"Ancestral origin and functional expression of a hyaluronic acid pathway complement in mussels","isi":1,"OA_type":"gold","acknowledgement":"This research was funded by the Italian Ministry of University and Research (MIUR), grant ID: P2022JEEMT (Developing a tool for the study of haplotype diversity in Mytilus galloprovincialis (HAMIGA)).","oa_version":"Published Version","has_accepted_license":"1","citation":{"chicago":"Rosani, Umberto, Nehir Altan, Paola Venier, Enrico Bortoletto, Nicola Volpi, and Carrie Bernecky. “Ancestral Origin and Functional Expression of a Hyaluronic Acid Pathway Complement in Mussels.” <i>Biology</i>. MDPI, 2025. <a href=\"https://doi.org/10.3390/biology14080930\">https://doi.org/10.3390/biology14080930</a>.","mla":"Rosani, Umberto, et al. “Ancestral Origin and Functional Expression of a Hyaluronic Acid Pathway Complement in Mussels.” <i>Biology</i>, vol. 14, no. 8, 930, MDPI, 2025, doi:<a href=\"https://doi.org/10.3390/biology14080930\">10.3390/biology14080930</a>.","short":"U. Rosani, N. Altan, P. Venier, E. Bortoletto, N. Volpi, C. Bernecky, Biology 14 (2025).","ama":"Rosani U, Altan N, Venier P, Bortoletto E, Volpi N, Bernecky C. Ancestral origin and functional expression of a hyaluronic acid pathway complement in mussels. <i>Biology</i>. 2025;14(8). doi:<a href=\"https://doi.org/10.3390/biology14080930\">10.3390/biology14080930</a>","ieee":"U. Rosani, N. Altan, P. Venier, E. Bortoletto, N. Volpi, and C. Bernecky, “Ancestral origin and functional expression of a hyaluronic acid pathway complement in mussels,” <i>Biology</i>, vol. 14, no. 8. MDPI, 2025.","ista":"Rosani U, Altan N, Venier P, Bortoletto E, Volpi N, Bernecky C. 2025. Ancestral origin and functional expression of a hyaluronic acid pathway complement in mussels. Biology. 14(8), 930.","apa":"Rosani, U., Altan, N., Venier, P., Bortoletto, E., Volpi, N., &#38; Bernecky, C. (2025). Ancestral origin and functional expression of a hyaluronic acid pathway complement in mussels. <i>Biology</i>. MDPI. <a href=\"https://doi.org/10.3390/biology14080930\">https://doi.org/10.3390/biology14080930</a>"},"OA_place":"publisher","status":"public","day":"24","type":"journal_article","publisher":"MDPI","article_number":"930","publication_identifier":{"issn":["2079-7737"]},"quality_controlled":"1","month":"07","PlanS_conform":"1","issue":"8","_id":"20077","DOAJ_listed":"1","article_processing_charge":"Yes","article_type":"original","author":[{"full_name":"Rosani, Umberto","last_name":"Rosani","first_name":"Umberto"},{"last_name":"Altan","full_name":"Altan, Nehir","first_name":"Nehir"},{"full_name":"Venier, Paola","last_name":"Venier","first_name":"Paola"},{"first_name":"Enrico","full_name":"Bortoletto, Enrico","last_name":"Bortoletto"},{"first_name":"Nicola","full_name":"Volpi, Nicola","last_name":"Volpi"},{"last_name":"Bernecky","full_name":"Bernecky, Carrie A","orcid":"0000-0003-0893-7036","id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","first_name":"Carrie A"}],"oa":1,"language":[{"iso":"eng"}],"department":[{"_id":"CaBe"}],"external_id":{"isi":["001557922100001"]},"publication_status":"published","doi":"10.3390/biology14080930","abstract":[{"lang":"eng","text":"Hyaluronic acid (HA) is a key extracellular matrix component of vertebrates, where it mediates cell adhesion, immune regulation, and tissue remodeling through its interaction with specific receptors. Although HA has been detected in a few invertebrate species, the lack of fundamental components of the molecular HA pathway poses relevant objections about its functional role in these species. Mining genomic and transcriptomic data, we considered the conservation of the gene locus encoding for the extracellular link protein (XLINK) in marine mussels as well as its expression patterns. Structural and phylogenetic analyses were undertaken to evaluate possible similarities with vertebrate orthologs and to infer the origin of this gene in invertebrates. Biochemical analysis was used to quantify HA in tissues of Mytilus galloprovincialis. As a result, we confirm that the mussel can produce HA (up to 1.02 ng/mg in mantle) and that its genome encodes two XLINK gene loci. These loci are conserved in Mytilidae species and show a complex evolutionary path. Mussel XLINK genes appeared to be expressed during developmental stages in three mussel species, ranking in the top 100 expressed genes in M. trossulus at 17 h post-fertilization. In conclusion, the presence of HA and an active gene with the potential to bind HA suggests that mussels have the potential to synthesize and use HA and are among the few invertebrates encoding this gene."}],"file_date_updated":"2025-07-31T09:11:09Z","publication":"Biology","file":[{"file_size":1885781,"file_name":"2025_Biology_Rosani.pdf","date_updated":"2025-07-31T09:11:09Z","access_level":"open_access","creator":"dernst","content_type":"application/pdf","success":1,"date_created":"2025-07-31T09:11:09Z","relation":"main_file","checksum":"f5e059e66803fa54249c1db029aef0f6","file_id":"20097"}],"intvolume":"        14","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2025-07-25T08:28:26Z","year":"2025","date_published":"2025-07-24T00:00:00Z","ddc":["570"],"date_updated":"2025-09-30T14:10:07Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"volume":14},{"intvolume":"        51","publication":"Reproductive Biomedicine Online","external_id":{"pmid":["40680553"],"isi":["001549819000002"]},"department":[{"_id":"SiHi"}],"publication_status":"published","doi":"10.1016/j.rbmo.2025.104942","abstract":[{"lang":"eng","text":"Research question: Is LINC01638 involved in regulation of epithelial-to-mesenchymal transition (EMT) in endometriosis?\r\nDesign: A prospective patient cohort study was combined with functional experiments in the 12Z endometriosis epithelial cell line to investigate the role of LINC01638 in endometriosis. Eutopic endometrial samples were collected by curettage, and ectopic endometrial lesion samples were collected by laparoscopic surgery from 24 control patients and 41 patients with endometriosis. The phenotype of 12Z cells was assessed following LINC01638 knockdown using siRNA, performing proliferation, adhesion, migration and invasion assays, as well as assessing apoptosis and cell cycle changes with flow cytometry assays. In order to assess the relationship between LINC01638 and histone deacetylase class 1 enzyme (HDAC1), LINC01638 knockdown was combined with HDAC inhibition with the specific HDAC inhibitor romidepsin.\r\nResults: LINC01638 was up-regulated in the epithelial layer of endometriotic lesions, and LINC01638 knockdown in 12Z cells led to reduced proliferation, adhesion, migration and invasion. The reduction in proliferation was associated with increased p21 and p27 expression, and G1 phase arrest. Further analysis of LINC01638 control and knockdown cells revealed that a number of transcription factors associated with EMT are down-regulated in knockdown cells, along with the cytoskeleton regulatory gene RHOB, while HDAC1 was up-regulated. Chromatin immunoprecipitation analysis and HDAC1 inhibitory treatment combined with LINC01638 knockdown indicated that LINC01638 regulates RHOB expression via HDAC1-mediated promoter deacetylation. RHOB is up-regulated in the epithelial layer of endometriotic lesions compared with eutopic endometrium, supporting a role in the disease.\r\nConclusions: LINC01638 is an epigenetic regulator of the pathogenesis of endometriosis, promoting proliferation and EMT of endometriotic lesions."}],"date_published":"2025-07-17T00:00:00Z","volume":51,"date_updated":"2025-09-30T14:10:46Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2025-07-27T22:01:25Z","year":"2025","pmid":1,"citation":{"chicago":"Yotova, Iveta, Katharina Proestling, Florian Pauler, Lisa Rainer, Leonie Kaup, Jana Heine, Lejla Sandrieser, René Wenzl, and Quanah J. Hudson. “LINC01638 Promotes Epithelial-to-Mesenchymal Transition in Endometriosis Epithelial Cells by up-Regulating RHOB via HDAC1 Suppression.” <i>Reproductive Biomedicine Online</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.rbmo.2025.104942\">https://doi.org/10.1016/j.rbmo.2025.104942</a>.","mla":"Yotova, Iveta, et al. “LINC01638 Promotes Epithelial-to-Mesenchymal Transition in Endometriosis Epithelial Cells by up-Regulating RHOB via HDAC1 Suppression.” <i>Reproductive Biomedicine Online</i>, vol. 51, no. 3, 104942, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.rbmo.2025.104942\">10.1016/j.rbmo.2025.104942</a>.","ista":"Yotova I, Proestling K, Pauler F, Rainer L, Kaup L, Heine J, Sandrieser L, Wenzl R, Hudson QJ. 2025. LINC01638 promotes epithelial-to-mesenchymal transition in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression. Reproductive Biomedicine Online. 51(3), 104942.","apa":"Yotova, I., Proestling, K., Pauler, F., Rainer, L., Kaup, L., Heine, J., … Hudson, Q. J. (2025). LINC01638 promotes epithelial-to-mesenchymal transition in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression. <i>Reproductive Biomedicine Online</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.rbmo.2025.104942\">https://doi.org/10.1016/j.rbmo.2025.104942</a>","ama":"Yotova I, Proestling K, Pauler F, et al. LINC01638 promotes epithelial-to-mesenchymal transition in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression. <i>Reproductive Biomedicine Online</i>. 2025;51(3). doi:<a href=\"https://doi.org/10.1016/j.rbmo.2025.104942\">10.1016/j.rbmo.2025.104942</a>","short":"I. Yotova, K. Proestling, F. Pauler, L. Rainer, L. Kaup, J. Heine, L. Sandrieser, R. Wenzl, Q.J. Hudson, Reproductive Biomedicine Online 51 (2025).","ieee":"I. Yotova <i>et al.</i>, “LINC01638 promotes epithelial-to-mesenchymal transition in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression,” <i>Reproductive Biomedicine Online</i>, vol. 51, no. 3. Elsevier, 2025."},"title":"LINC01638 promotes epithelial-to-mesenchymal transition in endometriosis epithelial cells by up-regulating RHOB via HDAC1 suppression","OA_type":"closed access","isi":1,"oa_version":"None","acknowledgement":"The authors wish to thank all the participants and health professionals involved in this study. In addition, the authors wish to thank technical assistants Barbara Widmar, Matthias Witzmann-Stern and Isabella Haslinger for their work assisting with this study; and Simon Hippenmeyer for access to bioinformatic infrastructure and resources.\r\nOpen access funding was provided by the Medical University of Vienna.","scopus_import":"1","_id":"20079","issue":"3","article_processing_charge":"No","article_type":"original","author":[{"first_name":"Iveta","full_name":"Yotova, Iveta","last_name":"Yotova"},{"first_name":"Katharina","last_name":"Proestling","full_name":"Proestling, Katharina"},{"first_name":"Florian","id":"48EA0138-F248-11E8-B48F-1D18A9856A87","full_name":"Pauler, Florian","orcid":"0000-0002-7462-0048","last_name":"Pauler"},{"first_name":"Lisa","full_name":"Rainer, Lisa","last_name":"Rainer"},{"first_name":"Leonie","last_name":"Kaup","full_name":"Kaup, Leonie"},{"last_name":"Heine","full_name":"Heine, Jana","first_name":"Jana"},{"full_name":"Sandrieser, Lejla","last_name":"Sandrieser","first_name":"Lejla"},{"full_name":"Wenzl, René","last_name":"Wenzl","first_name":"René"},{"first_name":"Quanah J.","full_name":"Hudson, Quanah J.","last_name":"Hudson"}],"language":[{"iso":"eng"}],"status":"public","type":"journal_article","day":"17","publication_identifier":{"eissn":["1472-6491"],"issn":["1472-6483"]},"publisher":"Elsevier","article_number":"104942","quality_controlled":"1","month":"07"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","date_created":"2025-07-27T22:01:26Z","project":[{"name":"Hormonal cross-talk in plant organogenesis","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","grant_number":"207362","call_identifier":"FP7"},{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"}],"ddc":["580"],"date_published":"2025-07-04T00:00:00Z","volume":16,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"APC_amount":"3642,79 EUR","date_updated":"2026-05-20T07:53:03Z","publication_status":"published","external_id":{"isi":["001530690900001"],"pmid":["40688689"]},"department":[{"_id":"EdHa"},{"_id":"EvBe"},{"_id":"CaGu"}],"ec_funded":1,"abstract":[{"text":"Introduction: Acid-growth theory has been postulated in the 70s to explain the rapid elongation of plant cells in response to the hormone auxin. More recently, it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting acidification of the apoplast is the principal mechanism by which auxin and other hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances, the impact of this acidification on the mechanical properties of the cell wall remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate hormone-induced tissue elongation and local changes in cell wall mechanical properties. Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase (PME), along with calcium chelators, we investigate the effect of pectin modification in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification of apoplast is necessary and sufficient to induce cell elongation through promoting cell wall softening. Moreover, we show that enhanced PME activity can induce both cell wall softening or stiffening in extracellular calcium dependent-manner and that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion: Our results confirm a dual role of PME in plant cell elongation. However, further investigation is needed to assess the status of pectin following short- or long-term PME treatments in order to determine if pectin methyl-esterification might promote its degradation as well as the role of PME inhibitors upon PME induction.","lang":"eng"}],"doi":"10.3389/fpls.2025.1612366","file_date_updated":"2025-07-31T07:28:54Z","intvolume":"        16","publication":"Frontiers in Plant Science","file":[{"date_created":"2025-07-31T07:28:54Z","content_type":"application/pdf","success":1,"checksum":"9e6b8b53ba56d4a24a9bd91cf6d2dc58","file_id":"20093","relation":"main_file","access_level":"open_access","file_size":3665187,"file_name":"2025_FrontiersPlantSc_Gallemi.pdf","date_updated":"2025-07-31T07:28:54Z","creator":"dernst"}],"type":"journal_article","day":"04","status":"public","OA_place":"publisher","month":"07","quality_controlled":"1","publisher":"Frontiers Media","article_number":"1612366","publication_identifier":{"eissn":["1664-462X"]},"article_processing_charge":"Yes","article_type":"original","_id":"20080","DOAJ_listed":"1","corr_author":"1","PlanS_conform":"1","language":[{"iso":"eng"}],"oa":1,"author":[{"id":"460C6802-F248-11E8-B48F-1D18A9856A87","last_name":"Gallemi","orcid":"0000-0003-4675-6893","full_name":"Gallemi, Marçal","first_name":"Marçal"},{"id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","last_name":"Montesinos López","full_name":"Montesinos López, Juan C","orcid":"0000-0001-9179-6099","first_name":"Juan C"},{"id":"18e95355-e05a-11ea-a9c0-8fba1b89e83a","last_name":"Zarevski","full_name":"Zarevski, Nikola","first_name":"Nikola"},{"first_name":"Jan","last_name":"Pribyl","full_name":"Pribyl, Jan"},{"first_name":"Petr","last_name":"Skládal","full_name":"Skládal, Petr"},{"first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"}],"title":"Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"}],"acknowledgement":"The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127). We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler for the efforts to establish immunodetection method.","scopus_import":"1","oa_version":"Published Version","OA_type":"gold","isi":1,"pmid":1,"citation":{"ista":"Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB, Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.","apa":"Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P., Hannezo, E. B., &#38; Benková, E. (2025). Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>","ieee":"M. Gallemi <i>et al.</i>, “Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties,” <i>Frontiers in Plant Science</i>, vol. 16. Frontiers Media, 2025.","short":"M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B. Hannezo, E. Benková, Frontiers in Plant Science 16 (2025).","ama":"Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. 2025;16. doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>","mla":"Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>, vol. 16, 1612366, Frontiers Media, 2025, doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>.","chicago":"Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl, Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>. Frontiers Media, 2025. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>."},"has_accepted_license":"1"},{"acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","oa_version":"Published Version","scopus_import":"1","OA_type":"hybrid","isi":1,"related_material":{"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/bench-pressing-cells/"}],"record":[{"relation":"dissertation_contains","status":"public","id":"20149"}]},"title":"Migrating immune cells globally coordinate protrusive forces","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"citation":{"mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>.","chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>.","apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>","ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.","ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. 2025;26:1258–1266. doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>","ieee":"P. Dos Reis Rodrigues <i>et al.</i>, “Migrating immune cells globally coordinate protrusive forces,” <i>Nature Immunology</i>, vol. 26. Springer Nature, pp. 1258–1266, 2025."},"has_accepted_license":"1","pmid":1,"month":"08","quality_controlled":"1","publisher":"Springer Nature","publication_identifier":{"eissn":["1529-2916"],"issn":["1529-2908"]},"day":"01","type":"journal_article","OA_place":"publisher","status":"public","page":"1258–1266","oa":1,"language":[{"iso":"eng"}],"author":[{"id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","orcid":"0000-0003-1681-508X","full_name":"Dos Reis Rodrigues, Patricia","last_name":"Dos Reis Rodrigues","first_name":"Patricia"},{"first_name":"Mario","full_name":"Avellaneda Sarrió, Mario","orcid":"0000-0001-6406-524X","last_name":"Avellaneda Sarrió","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E"},{"first_name":"Nikola","orcid":"0000-0002-8518-5926","full_name":"Canigova, Nikola","last_name":"Canigova","id":"3795523E-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-6120-3723","full_name":"Gärtner, Florian R","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R"},{"first_name":"Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87","full_name":"Vaahtomeri, Kari","orcid":"0000-0001-7829-3518","last_name":"Vaahtomeri"},{"id":"3BE60946-F248-11E8-B48F-1D18A9856A87","last_name":"Riedl","full_name":"Riedl, Michael","orcid":"0000-0003-4844-6311","first_name":"Michael"},{"first_name":"Ingrid","full_name":"De Vries, Ingrid","last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","last_name":"Merrin"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild","first_name":"Robert"},{"first_name":"Yoshinori","last_name":"Fukui","full_name":"Fukui, Yoshinori"},{"orcid":"0000-0002-1009-9652","full_name":"Juanes Garcia, Alba","last_name":"Juanes Garcia","id":"40F05888-F248-11E8-B48F-1D18A9856A87","first_name":"Alba"},{"last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K"}],"article_processing_charge":"Yes (via OA deal)","article_type":"letter_note","PlanS_conform":"1","corr_author":"1","_id":"20082","abstract":[{"text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence.","lang":"eng"}],"doi":"10.1038/s41590-025-02211-w","publication_status":"published","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"external_id":{"isi":["001529134300001"],"pmid":["40664976"]},"publication":"Nature Immunology","file":[{"access_level":"open_access","file_name":"2025_NatureImmunology_ReisRodrigues.pdf","file_size":13514646,"date_updated":"2025-07-31T08:00:33Z","creator":"dernst","date_created":"2025-07-31T08:00:33Z","success":1,"content_type":"application/pdf","checksum":"0c725123dca7797c682609bff2c4c5ac","file_id":"20096","relation":"main_file"}],"intvolume":"        26","file_date_updated":"2025-07-31T08:00:33Z","year":"2025","date_created":"2025-07-27T22:01:26Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-04-28T13:26:50Z","volume":26,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"date_published":"2025-08-01T00:00:00Z","project":[{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","grant_number":"101071793","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"},{"_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8","grant_number":"944-2020","name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration"}],"ddc":["570"]},{"author":[{"last_name":"Hwong","orcid":"0000-0001-9281-3479","full_name":"Hwong, Yi-Ling","id":"1217aa61-4dd1-11ec-9ac3-f2ba3f17ee22","first_name":"Yi-Ling"},{"last_name":"Byers","full_name":"Byers, Edward","first_name":"Edward"},{"full_name":"Werning, Michaela","last_name":"Werning","first_name":"Michaela"},{"first_name":"Yann","last_name":"Quilcaille","full_name":"Quilcaille, Yann"}],"oa":1,"language":[{"iso":"eng"}],"DOAJ_listed":"1","_id":"20098","corr_author":"1","issue":"3","PlanS_conform":"1","article_type":"original","article_processing_charge":"Yes","publisher":"IOP Publishing","publication_identifier":{"eissn":["2752-5295"]},"article_number":"035005","month":"07","quality_controlled":"1","status":"public","OA_place":"publisher","type":"journal_article","day":"15","has_accepted_license":"1","citation":{"ama":"Hwong Y-L, Byers E, Werning M, Quilcaille Y. Sustainable development key to limiting climate change-driven wildfire damages. <i>Environmental Research: Climate</i>. 2025;4(3). doi:<a href=\"https://doi.org/10.1088/2752-5295/adec11\">10.1088/2752-5295/adec11</a>","short":"Y.-L. Hwong, E. Byers, M. Werning, Y. Quilcaille, Environmental Research: Climate 4 (2025).","ieee":"Y.-L. Hwong, E. Byers, M. Werning, and Y. Quilcaille, “Sustainable development key to limiting climate change-driven wildfire damages,” <i>Environmental Research: Climate</i>, vol. 4, no. 3. IOP Publishing, 2025.","apa":"Hwong, Y.-L., Byers, E., Werning, M., &#38; Quilcaille, Y. (2025). Sustainable development key to limiting climate change-driven wildfire damages. <i>Environmental Research: Climate</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/2752-5295/adec11\">https://doi.org/10.1088/2752-5295/adec11</a>","ista":"Hwong Y-L, Byers E, Werning M, Quilcaille Y. 2025. Sustainable development key to limiting climate change-driven wildfire damages. Environmental Research: Climate. 4(3), 035005.","mla":"Hwong, Yi-Ling, et al. “Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages.” <i>Environmental Research: Climate</i>, vol. 4, no. 3, 035005, IOP Publishing, 2025, doi:<a href=\"https://doi.org/10.1088/2752-5295/adec11\">10.1088/2752-5295/adec11</a>.","chicago":"Hwong, Yi-Ling, Edward Byers, Michaela Werning, and Yann Quilcaille. “Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages.” <i>Environmental Research: Climate</i>. IOP Publishing, 2025. <a href=\"https://doi.org/10.1088/2752-5295/adec11\">https://doi.org/10.1088/2752-5295/adec11</a>."},"OA_type":"gold","oa_version":"Published Version","acknowledgement":"We thank Marina Andrijevic, Giacomo Falchetta, Samuel Lüthi, Caroline Muller, Carl Schleussner, and Adriano Vinca for providing useful ideas and feedback for this work. YLH is supported by funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska‐Curie Grant No. 101034413. EB, MW, and YQ are supported by the European Union’s Horizon Europe research and innovation programme under Grant Agreement No. 101081369 (SPARCCLE). We also thank the two anonymous reviewers for providing helpful feedback that greatly improved this manuscript.","scopus_import":"1","related_material":{"record":[{"status":"public","relation":"research_data","id":"20107"}]},"title":"Sustainable development key to limiting climate change-driven wildfire damages","volume":4,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"date_updated":"2025-08-04T07:46:33Z","ddc":["550"],"project":[{"_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413","call_identifier":"H2020","name":"IST-BRIDGE: International postdoctoral program"}],"date_published":"2025-07-15T00:00:00Z","date_created":"2025-07-31T14:03:16Z","year":"2025","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"         4","file":[{"checksum":"ca679496767021e792b0378c48fdee8c","file_id":"20108","relation":"main_file","date_created":"2025-08-04T07:38:14Z","success":1,"content_type":"application/pdf","creator":"dernst","access_level":"open_access","file_size":2807041,"date_updated":"2025-08-04T07:38:14Z","file_name":"2025_EnvironResearchClimate_Hwong.pdf"}],"publication":"Environmental Research: Climate","file_date_updated":"2025-08-04T07:38:14Z","doi":"10.1088/2752-5295/adec11","abstract":[{"text":"Climate change is causing wildfires to become more frequent and intense. While predicting burned areas using bioclimatic and anthropogenic factors is an active research area, few studies have examined what drives the economic damages of wildfires. Our study aims to fill this gap by analyzing key factors influencing global economic wildfire damages and projecting future damages under three shared socioeconomic pathways (SSPs). We apply regression analyses to identify significant predictors of economic wildfire damages at country levels and use the fitted model to project future damages under SSP126, SSP245, and SSP370. Results show that the human vulnerability index (HVI), reflecting socioeconomic conditions, is the strongest predictor of historical wildfire damages, followed by water vapor pressure deficit during the fire season and population density around forested areas. We found high population density to be associated with lower damages. These findings contrast with studies of burned areas, where climate factors are more dominant. Our model projects that by 2070, average global economic wildfire damages will be three times higher under SSP370 than SSP126. Our model also shows that following SSP126 not only reduces wildfire damages but also lessens the inequalities in damage distribution across countries. This pathway’s dual focus on equitable socioeconomic progress and climate action potentially enhances a country’s resilience that helps mitigate wildfire damages. Our analyses also indicate that strong socioeconomic development can offset wildfire damages associated with climate hazards, although this is less certain under SSP370. SSP126’s integrated approach improves both socioeconomic conditions and limits global warming, providing substantial benefits to less developed countries while still reducing damages in developed nations, despite their already low HVI scores. Our work complements existing research on burned areas and underscores the importance of sustainable development and international collaboration in reducing the economic damages of wildfires.","lang":"eng"}],"ec_funded":1,"department":[{"_id":"CaMu"}],"publication_status":"published"},{"publisher":"Elsevier","article_number":"116080","publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"month":"08","quality_controlled":"1","OA_place":"publisher","status":"public","day":"01","type":"journal_article","author":[{"first_name":"Jake","id":"63836096-4690-11EA-BD4E-32803DDC885E","last_name":"Watson","full_name":"Watson, Jake","orcid":"0000-0002-8698-3823"},{"first_name":"Victor M","last_name":"Vargas Barroso","full_name":"Vargas Barroso, Victor M","id":"2F55A9DE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Peter M","orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"language":[{"iso":"eng"}],"oa":1,"PlanS_conform":"1","corr_author":"1","_id":"20099","DOAJ_listed":"1","issue":"8","article_processing_charge":"Yes","article_type":"original","OA_type":"gold","isi":1,"scopus_import":"1","oa_version":"Published Version","acknowledgement":"We thank Andrea Navas-Olive and Rebecca J. Morse-Mora for critically reading an earlier version of the manuscript. We also thank Florian Marr and Christina Altmutter for excellent technical assistance, Alois Schlögl for programming and data-handling assistance, Todor Asenov for technical support, and Eleftheria Kralli-Beller for manuscript editing. This research was supported by the Scientific Services Units (SSUs) of ISTA. We are particularly grateful for assistance from the Imaging and Optics Facility, Preclinical Facility, Lab Support Facility, and Miba Machine Shop. The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 692692 to P.J., Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635 to J.F.W., and an ISTplus Fellowship through Marie Skłodowska-Curie grant agreement no. 754411 to V.V.-B.), the Austrian Science Fund (P 36232-B, PAT 4178023, and Cluster of Excellence 10.55776/COE16 to P.J.), and a CONACyT fellowship (289638 to V.V.-B.) and was supported by a non-stipendiary EMBO fellowship (ALTF 756–2020 to J.F.W.).","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"title":"Cell-specific wiring routes information flow through hippocampal CA3","has_accepted_license":"1","citation":{"chicago":"Watson, Jake, Victor M Vargas Barroso, and Peter M Jonas. “Cell-Specific Wiring Routes Information Flow through Hippocampal CA3.” <i>Cell Reports</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.116080\">https://doi.org/10.1016/j.celrep.2025.116080</a>.","mla":"Watson, Jake, et al. “Cell-Specific Wiring Routes Information Flow through Hippocampal CA3.” <i>Cell Reports</i>, vol. 44, no. 8, 116080, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116080\">10.1016/j.celrep.2025.116080</a>.","short":"J. Watson, V.M. Vargas Barroso, P.M. Jonas, Cell Reports 44 (2025).","ieee":"J. Watson, V. M. Vargas Barroso, and P. M. Jonas, “Cell-specific wiring routes information flow through hippocampal CA3,” <i>Cell Reports</i>, vol. 44, no. 8. Elsevier, 2025.","ama":"Watson J, Vargas Barroso VM, Jonas PM. Cell-specific wiring routes information flow through hippocampal CA3. <i>Cell Reports</i>. 2025;44(8). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116080\">10.1016/j.celrep.2025.116080</a>","apa":"Watson, J., Vargas Barroso, V. M., &#38; Jonas, P. M. (2025). Cell-specific wiring routes information flow through hippocampal CA3. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.116080\">https://doi.org/10.1016/j.celrep.2025.116080</a>","ista":"Watson J, Vargas Barroso VM, Jonas PM. 2025. Cell-specific wiring routes information flow through hippocampal CA3. Cell Reports. 44(8), 116080."},"date_created":"2025-08-03T22:01:30Z","year":"2025","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2025-09-30T14:12:02Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"volume":44,"date_published":"2025-08-01T00:00:00Z","project":[{"name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","call_identifier":"H2020","grant_number":"692692","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"},{"grant_number":"101026635","_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","call_identifier":"H2020","name":"Synaptic computations of the hippocampal CA3 circuitry"},{"name":"Mechanisms of GABA release in hippocampal circuits","grant_number":"P36232","_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5"},{"name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"ddc":["570"],"doi":"10.1016/j.celrep.2025.116080","ec_funded":1,"abstract":[{"lang":"eng","text":"The hippocampus, critical for learning and memory, is dogmatically described as a trisynaptic circuit where dentate gyrus granule cells (GCs), CA3 pyramidal neurons (PNs), and CA1 PNs are serially connected. However, CA3 also forms an autoassociative network, and its PNs have diverse morphologies, intrinsic properties, and GC input levels. How PN subtypes compose this recurrent network is unknown. To determine the synaptic arrangement of identified CA3 PNs, we combine multicellular patch-clamp recording and post hoc morphological analysis in mouse hippocampal slices. PNs can be divided into distinct “superficial” and “deep” subclasses, the latter including previously reported “athorny” cells. Subclasses have distinct input-output transformations and asymmetric connectivity, which is more abundant from superficial to deep PNs, splitting CA3 locally into two parallel recurrent networks. Coincident spontaneous inhibition occurs frequently within but not between subclasses, implying subclass-specific inhibitory innervation. Our results suggest two separately controlled sublayers for parallel information processing in hippocampal CA3."}],"department":[{"_id":"PeJo"}],"external_id":{"isi":["001544472300002"]},"publication_status":"published","intvolume":"        44","file":[{"access_level":"open_access","date_updated":"2025-08-04T06:53:07Z","file_size":27695214,"file_name":"2025_CellReports_Watson.pdf","creator":"dernst","date_created":"2025-08-04T06:53:07Z","success":1,"content_type":"application/pdf","checksum":"556ff9760661ecd23949d75031043b1f","file_id":"20106","relation":"main_file"}],"publication":"Cell Reports","file_date_updated":"2025-08-04T06:53:07Z"},{"year":"2025","date_created":"2025-08-03T22:01:31Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"volume":27,"date_updated":"2025-11-27T14:09:59Z","ddc":["000","570"],"date_published":"2025-06-27T00:00:00Z","abstract":[{"text":"A key step in protein structure prediction involves the detection of co-evolving pairs of residues, a signal for spatial proximity. This information is gleaned from multiple sequence alignment and underscores Alphafold’s structure prediction for almost every known protein. A simple means to create proteins beyond those found in nature, is by unnaturally fusing together two known proteins or protein parts. Here we demonstrate that structured peptides are predicted with significantly reduced accuracy when added to the terminal ends of scaffold proteins. Appending the multiple sequence alignment for the individual peptide tags to that of the scaffold protein often restores prediction accuracy. This work suggests that this windowed multiple sequence alignment approach can be a useful tool for predicting the structure of fused, chimeric proteins.","lang":"eng"}],"doi":"10.1016/j.csbj.2025.07.039","publication_status":"published","external_id":{"isi":["001583543100001"]},"department":[{"_id":"AlBr"}],"intvolume":"        27","file":[{"creator":"dernst","date_updated":"2025-08-04T06:25:23Z","file_size":6609770,"file_name":"2025_CompStrucBiotechJour_Vedula.pdf","access_level":"open_access","relation":"main_file","file_id":"20104","checksum":"78d01f30fc1dc11dd2bd1d7bb7ac8a62","success":1,"content_type":"application/pdf","date_created":"2025-08-04T06:25:23Z"}],"publication":"Computational and Structural Biotechnology Journal","file_date_updated":"2025-08-04T06:25:23Z","month":"06","quality_controlled":"1","publication_identifier":{"eissn":["2001-0370"]},"publisher":"Elsevier","type":"journal_article","day":"27","page":"3292-3298","status":"public","OA_place":"publisher","oa":1,"language":[{"iso":"eng"}],"author":[{"id":"94f2fe44-70fa-11f0-b76b-92922c09452b","last_name":"Vedula","full_name":"Vedula, Sanketh","first_name":"Sanketh"},{"id":"58f3726e-7cba-11ef-ad8b-e6e8cb3904e6","last_name":"Bronstein","orcid":"0000-0001-9699-8730","full_name":"Bronstein, Alexander","first_name":"Alexander"},{"first_name":"Ailie","full_name":"Marx, Ailie","last_name":"Marx"}],"article_type":"original","article_processing_charge":"Yes","_id":"20100","DOAJ_listed":"1","PlanS_conform":"1","acknowledgement":"AM acknowledges the financial support of the Helmsley Fellowships Program for Sustainability and Health. AMB is supported by the Schmidt Chair in Artificial Intelligence.","oa_version":"Published Version","scopus_import":"1","OA_type":"gold","isi":1,"related_material":{"record":[{"relation":"software","status":"public","id":"20103"}],"link":[{"url":"https://github.com/sankethvedula/AFChimera","relation":"software"}]},"title":"Improving prediction accuracy in chimeric proteins with windowed multiple sequence alignment","citation":{"mla":"Vedula, Sanketh, et al. “Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment.” <i>Computational and Structural Biotechnology Journal</i>, vol. 27, Elsevier, 2025, pp. 3292–98, doi:<a href=\"https://doi.org/10.1016/j.csbj.2025.07.039\">10.1016/j.csbj.2025.07.039</a>.","chicago":"Vedula, Sanketh, Alex M. Bronstein, and Ailie Marx. “Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment.” <i>Computational and Structural Biotechnology Journal</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.csbj.2025.07.039\">https://doi.org/10.1016/j.csbj.2025.07.039</a>.","ista":"Vedula S, Bronstein AM, Marx A. 2025. Improving prediction accuracy in chimeric proteins with windowed multiple sequence alignment. Computational and Structural Biotechnology Journal. 27, 3292–3298.","apa":"Vedula, S., Bronstein, A. M., &#38; Marx, A. (2025). Improving prediction accuracy in chimeric proteins with windowed multiple sequence alignment. <i>Computational and Structural Biotechnology Journal</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.csbj.2025.07.039\">https://doi.org/10.1016/j.csbj.2025.07.039</a>","short":"S. Vedula, A.M. Bronstein, A. Marx, Computational and Structural Biotechnology Journal 27 (2025) 3292–3298.","ama":"Vedula S, Bronstein AM, Marx A. Improving prediction accuracy in chimeric proteins with windowed multiple sequence alignment. <i>Computational and Structural Biotechnology Journal</i>. 2025;27:3292-3298. doi:<a href=\"https://doi.org/10.1016/j.csbj.2025.07.039\">10.1016/j.csbj.2025.07.039</a>","ieee":"S. Vedula, A. M. Bronstein, and A. Marx, “Improving prediction accuracy in chimeric proteins with windowed multiple sequence alignment,” <i>Computational and Structural Biotechnology Journal</i>, vol. 27. Elsevier, pp. 3292–3298, 2025."},"has_accepted_license":"1"},{"article_type":"original","article_processing_charge":"Yes (in subscription journal)","_id":"20102","issue":"21","PlanS_conform":"1","language":[{"iso":"eng"}],"oa":1,"author":[{"first_name":"Francesca","last_name":"Raffini","full_name":"Raffini, Francesca"},{"last_name":"De Jode","full_name":"De Jode, Aurélien","first_name":"Aurélien"},{"first_name":"Kerstin","full_name":"Johannesson, Kerstin","last_name":"Johannesson"},{"first_name":"Rui","last_name":"Faria","full_name":"Faria, Rui"},{"last_name":"Zagrodzka","full_name":"Zagrodzka, Zuzanna B.","first_name":"Zuzanna B."},{"first_name":"Anja M","last_name":"Westram","full_name":"Westram, Anja M","orcid":"0000-0003-1050-4969","id":"3C147470-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Juan","full_name":"Galindo, Juan","last_name":"Galindo"},{"last_name":"Rolán-Alvarez","full_name":"Rolán-Alvarez, Emilio","first_name":"Emilio"},{"full_name":"Butlin, Roger K.","last_name":"Butlin","first_name":"Roger K."}],"type":"journal_article","day":"01","status":"public","OA_place":"publisher","month":"11","quality_controlled":"1","publisher":"Wiley","article_number":"e70025","publication_identifier":{"eissn":["1365-294X"],"issn":["0962-1083"]},"citation":{"chicago":"Raffini, Francesca, Aurélien De Jode, Kerstin Johannesson, Rui Faria, Zuzanna B. Zagrodzka, Anja M Westram, Juan Galindo, Emilio Rolán-Alvarez, and Roger K. Butlin. “Phenotypic Divergence and Genomic Architecture between Parallel Ecotypes at Two Different Points on the Speciation Continuum in a Marine Snail.” <i>Molecular Ecology</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/mec.70025\">https://doi.org/10.1111/mec.70025</a>.","mla":"Raffini, Francesca, et al. “Phenotypic Divergence and Genomic Architecture between Parallel Ecotypes at Two Different Points on the Speciation Continuum in a Marine Snail.” <i>Molecular Ecology</i>, vol. 34, no. 21, e70025, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/mec.70025\">10.1111/mec.70025</a>.","ama":"Raffini F, De Jode A, Johannesson K, et al. Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail. <i>Molecular Ecology</i>. 2025;34(21). doi:<a href=\"https://doi.org/10.1111/mec.70025\">10.1111/mec.70025</a>","short":"F. Raffini, A. De Jode, K. Johannesson, R. Faria, Z.B. Zagrodzka, A.M. Westram, J. Galindo, E. Rolán-Alvarez, R.K. Butlin, Molecular Ecology 34 (2025).","ieee":"F. Raffini <i>et al.</i>, “Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail,” <i>Molecular Ecology</i>, vol. 34, no. 21. Wiley, 2025.","apa":"Raffini, F., De Jode, A., Johannesson, K., Faria, R., Zagrodzka, Z. B., Westram, A. M., … Butlin, R. K. (2025). Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail. <i>Molecular Ecology</i>. Wiley. <a href=\"https://doi.org/10.1111/mec.70025\">https://doi.org/10.1111/mec.70025</a>","ista":"Raffini F, De Jode A, Johannesson K, Faria R, Zagrodzka ZB, Westram AM, Galindo J, Rolán-Alvarez E, Butlin RK. 2025. Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail. Molecular Ecology. 34(21), e70025."},"has_accepted_license":"1","title":"Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail","oa_version":"Published Version","scopus_import":"1","acknowledgement":"This study was supported by European Research Council grant 693030-BARRIERS to RKB; the Swedish Research Council (grant number 2021-04191) to KJ; the Portuguese Foundation for Science and Technology (FCT: 2020.00275.CEECIND and PTDC/BIA-EVL/1614/2021) to RF; grant PID2022-137935NB-I00 by MICIU/AEI/ 10.13039/501100011033/and ERDF/EU (ED431C 2020-05) to JG, grant PID2021-124930NB-I00 funded by MICIU/AEI/ 10.13039/501100011033/and ERDF/EU to ERA, Xunta de Galicia (ED431C 2024/22), Centro singular de Investigación de Galicia accreditation 2024-2027 (ED431G 2023/07), ‘ERDF A way of making Europe’ and Norwegian Research Council RCN, project 315287 to AMW.","OA_type":"hybrid","isi":1,"ddc":["570"],"date_published":"2025-11-01T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"volume":34,"date_updated":"2025-12-30T09:25:45Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","date_created":"2025-08-03T22:01:31Z","file_date_updated":"2025-12-30T09:25:17Z","publication":"Molecular Ecology","file":[{"date_created":"2025-12-30T09:25:17Z","content_type":"application/pdf","success":1,"checksum":"ec01edda64cfbc6cbc8adf300f719644","file_id":"20906","relation":"main_file","access_level":"open_access","file_size":2767745,"file_name":"2025_MolecEcology_Raffini.pdf","date_updated":"2025-12-30T09:25:17Z","creator":"dernst"}],"intvolume":"        34","publication_status":"published","external_id":{"isi":["001538172800001"]},"department":[{"_id":"NiBa"}],"abstract":[{"lang":"eng","text":"Speciation is rarely observable directly. A way forward is to compare pairs of ecotypes that evolved in parallel in similar contexts but have reached different degrees of reproductive isolation. Such comparisons are possible in the marine snail Littorina saxatilis by contrasting barriers to gene flow between parallel ecotypes in Spain and Sweden. In both countries, divergent ecotypes have evolved to withstand either crab predation or wave action. Here, we explore transects spanning contact zones between the Crab and the Wave ecotypes using low-coverage whole-genome sequencing, morphological and behavioural traits. Despite parallel phenotypic divergence, distinct patterns of differentiation between the ecotypes emerged: a continuous cline in Sweden indicating a weak barrier to gene flow, but two highly genetically and phenotypically divergent, and partly spatially overlapping clusters in Spain suggesting a much stronger barrier to gene flow. The absence of Spanish early-generation hybrids supported strong isolation, but a low level of gene flow is evident from molecular data. In both countries, highly differentiated loci were located in both shared and country-specific chromosomal inversions but were also present in collinear regions. Despite being considered the same species and showing similar levels of phenotypic divergence, the Spanish ecotypes are much closer to full reproductive isolation than the Swedish ones. Barriers to gene flow of very different strengths between ecotypes within the same species might be explained by dissimilarities in the spatial arrangement of habitats, the selection gradients or the ages of the systems."}],"doi":"10.1111/mec.70025"},{"abstract":[{"text":"Official implementation, windowed MSAs, and the predictions as reported in the manuscript titled \"Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment\". (2025-06-27)","lang":"eng"}],"oa_version":"Published Version","doi":"10.7910/DVN/DYEBVM","title":"Replication Data for: \"Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment\"","related_material":{"record":[{"id":"20100","relation":"used_for_analysis_in","status":"public"}]},"department":[{"_id":"AlBr"}],"citation":{"ieee":"S. Vedula, A. M. Bronstein, and A. Marx, “Replication Data for: ‘Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment.’” Harvard Dataverse, 2025.","short":"S. Vedula, A.M. Bronstein, A. Marx, (2025).","ama":"Vedula S, Bronstein AM, Marx A. Replication Data for: “Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment.” 2025. doi:<a href=\"https://doi.org/10.7910/DVN/DYEBVM\">10.7910/DVN/DYEBVM</a>","apa":"Vedula, S., Bronstein, A. M., &#38; Marx, A. (2025). Replication Data for: “Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment.” Harvard Dataverse. <a href=\"https://doi.org/10.7910/DVN/DYEBVM\">https://doi.org/10.7910/DVN/DYEBVM</a>","ista":"Vedula S, Bronstein AM, Marx A. 2025. Replication Data for: ‘Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment’, Harvard Dataverse, <a href=\"https://doi.org/10.7910/DVN/DYEBVM\">10.7910/DVN/DYEBVM</a>.","mla":"Vedula, Sanketh, et al. <i>Replication Data for: “Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment.”</i> Harvard Dataverse, 2025, doi:<a href=\"https://doi.org/10.7910/DVN/DYEBVM\">10.7910/DVN/DYEBVM</a>.","chicago":"Vedula, Sanketh, Alex M. Bronstein, and Ailie Marx. “Replication Data for: ‘Improving Prediction Accuracy in Chimeric Proteins with Windowed Multiple Sequence Alignment.’” Harvard Dataverse, 2025. <a href=\"https://doi.org/10.7910/DVN/DYEBVM\">https://doi.org/10.7910/DVN/DYEBVM</a>."},"has_accepted_license":"1","year":"2025","month":"06","main_file_link":[{"open_access":"1","url":"https://doi.org/10.7910/DVN/DYEBVM"}],"publisher":"Harvard Dataverse","date_created":"2025-08-04T06:18:55Z","type":"research_data_reference","day":"27","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","OA_place":"repository","tmp":{"legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","short":"CC0 (1.0)","name":"Creative Commons Public Domain Dedication (CC0 1.0)","image":"/images/cc_0.png"},"oa":1,"date_updated":"2025-11-27T14:09:58Z","author":[{"id":"94f2fe44-70fa-11f0-b76b-92922c09452b","last_name":"Vedula","full_name":"Vedula, Sanketh","first_name":"Sanketh"},{"first_name":"Alexander","last_name":"Bronstein","full_name":"Bronstein, Alexander","orcid":"0000-0001-9699-8730","id":"58f3726e-7cba-11ef-ad8b-e6e8cb3904e6"},{"first_name":"Ailie","full_name":"Marx, Ailie","last_name":"Marx"}],"ddc":["000"],"article_processing_charge":"No","date_published":"2025-06-27T00:00:00Z","_id":"20103"},{"corr_author":"1","_id":"20107","project":[{"name":"IST-BRIDGE: International postdoctoral program","call_identifier":"H2020","grant_number":"101034413","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c"}],"ddc":["550"],"article_processing_charge":"No","date_published":"2025-05-21T00:00:00Z","author":[{"first_name":"Yi-Ling","last_name":"Hwong","orcid":"0000-0001-9281-3479","full_name":"Hwong, Yi-Ling","id":"1217aa61-4dd1-11ec-9ac3-f2ba3f17ee22"},{"full_name":"Byers, Edward","last_name":"Byers","first_name":"Edward"},{"first_name":"Michaela","last_name":"Werning","full_name":"Werning, Michaela"},{"first_name":"Yann","full_name":"Quilcaille, Yann","last_name":"Quilcaille"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"oa":1,"date_updated":"2025-08-04T07:46:33Z","status":"public","OA_place":"repository","type":"research_data_reference","day":"21","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Zenodo","main_file_link":[{"open_access":"1","url":"https://doi.org/10.5281/zenodo.15409324"}],"date_created":"2025-08-04T07:34:39Z","year":"2025","month":"05","has_accepted_license":"1","citation":{"mla":"Hwong, Yi-Ling, et al. <i>Data - Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages</i>. Zenodo, 2025, doi:<a href=\"https://doi.org/10.5281/ZENODO.13988679\">10.5281/ZENODO.13988679</a>.","chicago":"Hwong, Yi-Ling, Edward Byers, Michaela Werning, and Yann Quilcaille. “Data - Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages.” Zenodo, 2025. <a href=\"https://doi.org/10.5281/ZENODO.13988679\">https://doi.org/10.5281/ZENODO.13988679</a>.","ama":"Hwong Y-L, Byers E, Werning M, Quilcaille Y. Data - Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages. 2025. doi:<a href=\"https://doi.org/10.5281/ZENODO.13988679\">10.5281/ZENODO.13988679</a>","ieee":"Y.-L. Hwong, E. Byers, M. Werning, and Y. Quilcaille, “Data - Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages.” Zenodo, 2025.","short":"Y.-L. Hwong, E. Byers, M. Werning, Y. Quilcaille, (2025).","apa":"Hwong, Y.-L., Byers, E., Werning, M., &#38; Quilcaille, Y. (2025). Data - Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages. Zenodo. <a href=\"https://doi.org/10.5281/ZENODO.13988679\">https://doi.org/10.5281/ZENODO.13988679</a>","ista":"Hwong Y-L, Byers E, Werning M, Quilcaille Y. 2025. Data - Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages, Zenodo, <a href=\"https://doi.org/10.5281/ZENODO.13988679\">10.5281/ZENODO.13988679</a>."},"department":[{"_id":"CaMu"}],"title":"Data - Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages","related_material":{"record":[{"id":"20098","status":"public","relation":"used_in_publication"}]},"OA_type":"green","doi":"10.5281/ZENODO.13988679","abstract":[{"text":"This repository contains the data and scripts required to reproduce the results of the manuscript \"Sustainable Development Key to Limiting Climate Change-Driven Wildfire Damages\" submitted to the Environmental Research Climate Journal (ERCL). ","lang":"eng"}],"ec_funded":1,"oa_version":"Published Version"},{"abstract":[{"lang":"eng","text":"Auxin regulates various aspects of plant growth and development by modulating the transcription of target genes through the degradation of auxin/indole-3-acetic acid (Aux/IAA) repressors via the 26S proteasome. Proteasome regulator 1 (PTRE1), a positive regulator of proteasome activity, has been implicated in auxin-mediated proteasome suppression; however, the mechanism by which auxin modulates PTRE1 function remains unclear. Here, we demonstrate that auxin promotes the interaction between germin-like protein 1 (GLP1) and PTRE1, facilitating PTRE1 retention at the plasma membrane. The relocation of PTRE1 results in reduced nuclear 26S proteasome activity, and thus the attenuated Aux/IAA degradation and altered Aux/IAA homeostasis, ultimately resulting in suppressed auxin-mediated transcriptional regulation. Our findings uncover a previously uncharacterized regulatory axis in auxin signaling that controls Aux/IAA protein stability, functioning alongside the TIR1- and TRANSMEMBRANE KINASE 1 (TMK1)-mediated pathways, and highlight the coordination of auxin signaling from the cell surface to the nucleus via auxin-induced PTRE1 relocation, which fine-tunes Aux/IAA protein homeostasis and auxin responses."}],"doi":"10.1016/j.celrep.2025.116056","publication_status":"published","external_id":{"isi":["001542038500001"],"pmid":["40714631"]},"department":[{"_id":"JiFr"}],"file":[{"file_name":"2025_CellReports_Xu.pdf","date_updated":"2025-08-05T06:15:09Z","file_size":24178018,"access_level":"open_access","creator":"dernst","content_type":"application/pdf","success":1,"date_created":"2025-08-05T06:15:09Z","relation":"main_file","checksum":"3c43e040a4a7a65ec67ae1d2bb81261a","file_id":"20120"}],"intvolume":"        44","publication":"Cell Reports","file_date_updated":"2025-08-05T06:15:09Z","year":"2025","date_created":"2025-08-04T13:39:11Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)"},"volume":44,"date_updated":"2025-09-30T14:13:45Z","ddc":["580"],"date_published":"2025-07-24T00:00:00Z","acknowledgement":"The study was supported by the National Natural Science Foundation of China (NSFC; 32230011, 91954206, and 31721001). We thank Dr. Deli Lin (Shanghai Jiao Tong University) for kind help with the laser confocal microscope observation and the Arabidopsis Biological Resource Center (ABRC) for providing T-DNA insertional mutants.","scopus_import":"1","oa_version":"Published Version","isi":1,"OA_type":"gold","title":"Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis","citation":{"chicago":"Xu, Faqing, Yongqiang Yu, Bin Guan, Tongda Xu, Zhihong Xu, and Hongwei Xue. “Germin-like Protein 1 Interacts with Proteasome Regulator 1 to Regulate Auxin Signaling by Controlling Aux/IAA Homeostasis.” <i>Cell Reports</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.116056\">https://doi.org/10.1016/j.celrep.2025.116056</a>.","mla":"Xu, Faqing, et al. “Germin-like Protein 1 Interacts with Proteasome Regulator 1 to Regulate Auxin Signaling by Controlling Aux/IAA Homeostasis.” <i>Cell Reports</i>, vol. 44, no. 8, 116056, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116056\">10.1016/j.celrep.2025.116056</a>.","short":"F. Xu, Y. Yu, B. Guan, T. Xu, Z. Xu, H. Xue, Cell Reports 44 (2025).","ieee":"F. Xu, Y. Yu, B. Guan, T. Xu, Z. Xu, and H. Xue, “Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis,” <i>Cell Reports</i>, vol. 44, no. 8. Elsevier, 2025.","ama":"Xu F, Yu Y, Guan B, Xu T, Xu Z, Xue H. Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis. <i>Cell Reports</i>. 2025;44(8). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116056\">10.1016/j.celrep.2025.116056</a>","ista":"Xu F, Yu Y, Guan B, Xu T, Xu Z, Xue H. 2025. Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis. Cell Reports. 44(8), 116056.","apa":"Xu, F., Yu, Y., Guan, B., Xu, T., Xu, Z., &#38; Xue, H. (2025). Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.116056\">https://doi.org/10.1016/j.celrep.2025.116056</a>"},"has_accepted_license":"1","pmid":1,"quality_controlled":"1","month":"07","publisher":"Elsevier","article_number":"116056","publication_identifier":{"eissn":["2211-1247"]},"type":"journal_article","day":"24","status":"public","OA_place":"publisher","language":[{"iso":"eng"}],"oa":1,"author":[{"first_name":"Faqing","full_name":"Xu, Faqing","last_name":"Xu"},{"full_name":"Yu, Yongqiang","last_name":"Yu","first_name":"Yongqiang"},{"id":"56aad729-cca2-11ed-a45a-9b4138991a48","last_name":"Guan","full_name":"Guan, Bin","first_name":"Bin"},{"first_name":"Tongda","last_name":"Xu","full_name":"Xu, Tongda"},{"first_name":"Zhihong","full_name":"Xu, Zhihong","last_name":"Xu"},{"first_name":"Hongwei","last_name":"Xue","full_name":"Xue, Hongwei"}],"article_processing_charge":"Yes","article_type":"original","issue":"8","_id":"20116","DOAJ_listed":"1"},{"supervisor":[{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva"}],"author":[{"first_name":"Yiqun","id":"82F537F2-B517-11E9-84D7-6433E6697425","last_name":"Wang","full_name":"Wang, Yiqun"}],"language":[{"iso":"eng"}],"_id":"20117","corr_author":"1","article_processing_charge":"No","publication_identifier":{"issn":["2663-337X"]},"publisher":"Institute of Science and Technology Austria","month":"08","status":"public","page":"108","OA_place":"publisher","type":"dissertation","day":"04","has_accepted_license":"1","citation":{"mla":"Wang, Yiqun. <i>The Role of Dynamin Related Protein 2A in Cytokinin Regulated Plant Growth and Development</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20117\">10.15479/AT-ISTA-20117</a>.","chicago":"Wang, Yiqun. “The Role of Dynamin Related Protein 2A in Cytokinin Regulated Plant Growth and Development.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20117\">https://doi.org/10.15479/AT-ISTA-20117</a>.","ama":"Wang Y. The role of dynamin related protein 2A in cytokinin regulated plant growth and development. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20117\">10.15479/AT-ISTA-20117</a>","short":"Y. Wang, The Role of Dynamin Related Protein 2A in Cytokinin Regulated Plant Growth and Development, Institute of Science and Technology Austria, 2025.","ieee":"Y. Wang, “The role of dynamin related protein 2A in cytokinin regulated plant growth and development,” Institute of Science and Technology Austria, 2025.","ista":"Wang Y. 2025. The role of dynamin related protein 2A in cytokinin regulated plant growth and development. Institute of Science and Technology Austria.","apa":"Wang, Y. (2025). <i>The role of dynamin related protein 2A in cytokinin regulated plant growth and development</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20117\">https://doi.org/10.15479/AT-ISTA-20117</a>"},"acknowledgement":"I would also like to acknowledge the invaluable assistance provided by the Plant\r\nFacility, Imaging & Optics Facility, and the Lab Support Facility. The technical support and\r\nresources offered by these facilities were indispensable to the successful completion of my\r\nexperiments.","oa_version":"Published Version","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"18063"}]},"title":"The role of dynamin related protein 2A in cytokinin regulated plant growth and development","date_updated":"2026-04-07T11:49:34Z","ddc":["580"],"date_published":"2025-08-04T00:00:00Z","date_created":"2025-08-04T15:24:21Z","year":"2025","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","file":[{"file_size":25798848,"file_name":"2025_Wang_Yiqun_Thesis.docx","date_updated":"2025-08-22T08:53:46Z","access_level":"closed","creator":"yiqwang","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2025-08-22T08:22:10Z","relation":"source_file","file_id":"20209","checksum":"36b87c17d12c7bf5955d6d812acb8d77"},{"file_id":"20211","checksum":"8d7a2383f66377da675d379ec30ea0fe","relation":"main_file","date_created":"2025-08-22T10:32:30Z","content_type":"application/pdf","creator":"yiqwang","embargo_to":"open_access","access_level":"closed","embargo":"2026-09-03","date_updated":"2025-09-03T09:36:52Z","file_name":"2025_Wang_Yiqun_Thesis.pdf","file_size":12628313}],"file_date_updated":"2025-09-03T09:36:52Z","alternative_title":["ISTA Thesis"],"doi":"10.15479/AT-ISTA-20117","degree_awarded":"PhD","department":[{"_id":"GradSch"},{"_id":"EvBe"}],"publication_status":"published"},{"has_accepted_license":"1","citation":{"chicago":"Zhang, Zhiying, Thomas C. Todeschini, Yi Wu, Roman Kogay, Ameena Naji, Joaquin Cardenas Rodriguez, Rupavidhya Mondi, et al. “Kiwa Is a Membrane-Embedded Defense Supercomplex Activated at Phage Attachment Sites.” <i>Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.cell.2025.07.002\">https://doi.org/10.1016/j.cell.2025.07.002</a>.","mla":"Zhang, Zhiying, et al. “Kiwa Is a Membrane-Embedded Defense Supercomplex Activated at Phage Attachment Sites.” <i>Cell</i>, vol. 188, no. 21, Elsevier, 2025, p. 5862–5877.e23, doi:<a href=\"https://doi.org/10.1016/j.cell.2025.07.002\">10.1016/j.cell.2025.07.002</a>.","apa":"Zhang, Z., Todeschini, T. C., Wu, Y., Kogay, R., Naji, A., Cardenas Rodriguez, J., … Nobrega, F. L. (2025). Kiwa is a membrane-embedded defense supercomplex activated at phage attachment sites. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2025.07.002\">https://doi.org/10.1016/j.cell.2025.07.002</a>","ista":"Zhang Z, Todeschini TC, Wu Y, Kogay R, Naji A, Cardenas Rodriguez J, Mondi R, Kaganovich D, Taylor DW, Bravo JPK, Teplova M, Amen T, Koonin E, Patel DJ, Nobrega FL. 2025. Kiwa is a membrane-embedded defense supercomplex activated at phage attachment sites. Cell. 188(21), 5862–5877.e23.","short":"Z. Zhang, T.C. Todeschini, Y. Wu, R. Kogay, A. Naji, J. Cardenas Rodriguez, R. Mondi, D. Kaganovich, D.W. Taylor, J.P.K. Bravo, M. Teplova, T. Amen, E. Koonin, D.J. Patel, F.L. Nobrega, Cell 188 (2025) 5862–5877.e23.","ieee":"Z. Zhang <i>et al.</i>, “Kiwa is a membrane-embedded defense supercomplex activated at phage attachment sites,” <i>Cell</i>, vol. 188, no. 21. Elsevier, p. 5862–5877.e23, 2025.","ama":"Zhang Z, Todeschini TC, Wu Y, et al. Kiwa is a membrane-embedded defense supercomplex activated at phage attachment sites. <i>Cell</i>. 2025;188(21):5862-5877.e23. doi:<a href=\"https://doi.org/10.1016/j.cell.2025.07.002\">10.1016/j.cell.2025.07.002</a>"},"pmid":1,"isi":1,"OA_type":"hybrid","oa_version":"Published Version","acknowledgement":"We thank Rotem Sorek (Weizmann Institute of Science) for the Lambda Gam mutant and Ian Molineux (University of Texas) for T4Δgp2. We thank You Yu (Zhejiang University-University of Edinburgh Institute) and J. De La Cruz (MSK) for assistance with cryo-EM data collection and Lyuqin Zheng (MSK) for discussions on structural analysis. We thank the Imaging and Microscopy Centre (IMC) at the University of Southampton. This work was supported by Royal Society grant RGS\\R2\\222312 to F.L.N.; Welch Foundation grant F-1938 and National Institutes of Health R35GM138348 to D.W.T.; Wessex Medical Research Innovation grant AE06 to T.A.; and NIH grant GM145888 and Maloris Foundation and Memorial Sloan-Kettering Core grant (P30-CA008748) to D.J.P. In addition to MSKCC cryo-EM resources, some of this work was performed at the National Center for CryoEM Access and Training (NCCAT) and the Simons Electron Microscopy Center located at the New York Structural Biology Center, supported by the NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program (U24 GM129539) and Simons Foundation (SF349247) and NY State Assembly grants. This research used NSLS-II MX X-ray User Resources (FMX) of the National Synchrotron Light Source II, operated for the DOE Office of Science by Brookhaven National Laboratory under contract no. DE-SC0012704. The Center for BioMolecular Structure (CBMS) is primarily supported by the NIH, the National Institute of General Medical Sciences (NIGMS) through a Center Core P30 Grant (P30GM133893), and by the DOE Office of Biological and Environmental Research (KP1605010). R.K. and E.V.K. are supported by the Intramural Research Program of the NIH (National Library of Medicine).","scopus_import":"1","title":"Kiwa is a membrane-embedded defense supercomplex activated at phage attachment sites","author":[{"last_name":"Zhang","full_name":"Zhang, Zhiying","first_name":"Zhiying"},{"last_name":"Todeschini","full_name":"Todeschini, Thomas C.","first_name":"Thomas C."},{"last_name":"Wu","full_name":"Wu, Yi","first_name":"Yi"},{"last_name":"Kogay","full_name":"Kogay, Roman","first_name":"Roman"},{"first_name":"Ameena","last_name":"Naji","full_name":"Naji, Ameena"},{"full_name":"Cardenas Rodriguez, Joaquin","last_name":"Cardenas Rodriguez","first_name":"Joaquin"},{"first_name":"Rupavidhya","last_name":"Mondi","full_name":"Mondi, Rupavidhya"},{"first_name":"Daniel","full_name":"Kaganovich, Daniel","last_name":"Kaganovich"},{"full_name":"Taylor, David W.","last_name":"Taylor","first_name":"David W."},{"last_name":"Bravo","full_name":"Bravo, Jack Peter Kelly","orcid":"0000-0003-0456-0753","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","first_name":"Jack Peter Kelly"},{"first_name":"Marianna","last_name":"Teplova","full_name":"Teplova, Marianna"},{"last_name":"Amen","full_name":"Amen, Triana","first_name":"Triana"},{"last_name":"Koonin","full_name":"Koonin, Eugene","first_name":"Eugene"},{"last_name":"Patel","full_name":"Patel, Dinshaw J.","first_name":"Dinshaw J."},{"full_name":"Nobrega, Franklin L.","last_name":"Nobrega","first_name":"Franklin L."}],"language":[{"iso":"eng"}],"oa":1,"issue":"21","_id":"20143","PlanS_conform":"1","article_type":"original","article_processing_charge":"Yes (in subscription journal)","publisher":"Elsevier","publication_identifier":{"issn":["0092-8674"],"eissn":["1097-4172"]},"quality_controlled":"1","month":"10","page":"5862-5877.e23","status":"public","OA_place":"publisher","type":"journal_article","day":"16","file":[{"date_updated":"2025-12-29T14:15:25Z","file_name":"2025_Cell_Zhang.pdf","file_size":32104588,"access_level":"open_access","creator":"dernst","success":1,"content_type":"application/pdf","date_created":"2025-12-29T14:15:25Z","relation":"main_file","checksum":"b944de5fbd7455f58e1ff338ad352239","file_id":"20875"}],"intvolume":"       188","publication":"Cell","file_date_updated":"2025-12-29T14:15:25Z","doi":"10.1016/j.cell.2025.07.002","abstract":[{"text":"Bacteria and archaea deploy diverse antiviral defense systems, many of which remain mechanistically uncharacterized. Here, we characterize Kiwa, a widespread two-component system composed of the transmembrane sensor KwaA and the DNA-binding effector KwaB. Cryogenic electron microscopy (cryo-EM) analysis reveals that KwaA and KwaB assemble into a large, membrane-associated supercomplex. Upon phage binding, KwaA senses infection at the membrane, leading to KwaB binding of ejected phage DNA and inhibition of replication and late transcription, without inducing host cell death. Although KwaB can bind DNA independently, its antiviral activity requires association with KwaA, suggesting spatial or conformational regulation. We show that the phage-encoded DNA-mimic protein Gam directly binds and inhibits KwaB but that co-expression with the Gam-targeted RecBCD system restores protection by Kiwa. Our findings support a model in which Kiwa coordinates membrane-associated detection of phage infection with downstream DNA binding by its effector, forming a spatially coordinated antiviral mechanism.","lang":"eng"}],"external_id":{"isi":["001603560700005"],"pmid":["40730155"]},"department":[{"_id":"JaBr"}],"publication_status":"published","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"volume":188,"date_updated":"2025-12-29T14:15:58Z","ddc":["570"],"date_published":"2025-10-16T00:00:00Z","date_created":"2025-08-07T05:00:04Z","year":"2025","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"OA_type":"gold","doi":"10.5281/zenodo.15269364","abstract":[{"lang":"eng","text":"This criteria catalogue and the accompanying assessment questions were developed by a working group of KEMÖ (Kooperation E-Medien Österreich, the Austrian Academic Library Consortium). They are intended to support research institutions and organisations in the evaluation of Open Science Infrastructures. The 20 criteria outlined in the catalogue provide a structured basis for making informed decisions regarding the financial support of these infrastructures.\r\n\r\nThe assessment questions are intended to be completed by Open Science Infrastructures and can be shared with them accordingly."}],"oa_version":"Published Version","department":[{"_id":"E-Lib"}],"title":"Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures","publication_status":"published","has_accepted_license":"1","citation":{"ieee":"P. Gredler <i>et al.</i>, <i>Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures</i>. Zenodo, 2025.","short":"P. Gredler, C. Kaier, P. Danowski, M. Zoyer, K. Rieck, A. Ferus, E. Rosenberger, A. Löffler, L. Hofer, L. Still, Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures, Zenodo, 2025.","ama":"Gredler P, Kaier C, Danowski P, et al. <i>Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures</i>. Zenodo; 2025. doi:<a href=\"https://doi.org/10.5281/zenodo.15269364\">10.5281/zenodo.15269364</a>","apa":"Gredler, P., Kaier, C., Danowski, P., Zoyer, M., Rieck, K., Ferus, A., … Still, L. (2025). <i>Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures</i>. Zenodo. <a href=\"https://doi.org/10.5281/zenodo.15269364\">https://doi.org/10.5281/zenodo.15269364</a>","ista":"Gredler P, Kaier C, Danowski P, Zoyer M, Rieck K, Ferus A, Rosenberger E, Löffler A, Hofer L, Still L. 2025. Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures, Zenodo,p.","mla":"Gredler, Paul, et al. <i>Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures</i>. Zenodo, 2025, doi:<a href=\"https://doi.org/10.5281/zenodo.15269364\">10.5281/zenodo.15269364</a>.","chicago":"Gredler, Paul, Christian Kaier, Patrick Danowski, Michael Zoyer, Katharina Rieck, Andreas Ferus, Elisabeth Rosenberger, Alexander Löffler, Lisa Hofer, and Laura Still. <i>Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures</i>. Zenodo, 2025. <a href=\"https://doi.org/10.5281/zenodo.15269364\">https://doi.org/10.5281/zenodo.15269364</a>."},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.5281/zenodo.15269364"}],"publisher":"Zenodo","date_created":"2025-08-07T11:10:14Z","year":"2025","month":"08","status":"public","OA_place":"publisher","type":"working_paper","day":"07","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Paul","last_name":"Gredler","full_name":"Gredler, Paul"},{"first_name":"Christian","last_name":"Kaier","full_name":"Kaier, Christian"},{"first_name":"Patrick","last_name":"Danowski","full_name":"Danowski, Patrick","orcid":"0000-0002-6026-4409","id":"2EBD1598-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Michael","last_name":"Zoyer","full_name":"Zoyer, Michael"},{"first_name":"Katharina","last_name":"Rieck","full_name":"Rieck, Katharina"},{"full_name":"Ferus, Andreas","last_name":"Ferus","first_name":"Andreas"},{"full_name":"Rosenberger, Elisabeth","last_name":"Rosenberger","first_name":"Elisabeth"},{"full_name":"Löffler, Alexander","last_name":"Löffler","first_name":"Alexander"},{"first_name":"Lisa","last_name":"Hofer","full_name":"Hofer, Lisa"},{"last_name":"Still","full_name":"Still, Laura","first_name":"Laura"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"language":[{"iso":"eng"}],"oa":1,"date_updated":"2025-08-11T07:20:03Z","_id":"20146","ddc":["020"],"article_processing_charge":"No","date_published":"2025-08-07T00:00:00Z"},{"citation":{"mla":"Dos Reis Rodrigues, Patricia. <i>Coordination of Protrusive Forces in Immune Cell Migration </i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20149\">10.15479/AT-ISTA-20149</a>.","chicago":"Dos Reis Rodrigues, Patricia. “Coordination of Protrusive Forces in Immune Cell Migration .” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20149\">https://doi.org/10.15479/AT-ISTA-20149</a>.","ista":"Dos Reis Rodrigues P. 2025. Coordination of protrusive forces in immune cell migration . Institute of Science and Technology Austria.","apa":"Dos Reis Rodrigues, P. (2025). <i>Coordination of protrusive forces in immune cell migration </i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20149\">https://doi.org/10.15479/AT-ISTA-20149</a>","short":"P. Dos Reis Rodrigues, Coordination of Protrusive Forces in Immune Cell Migration , Institute of Science and Technology Austria, 2025.","ieee":"P. Dos Reis Rodrigues, “Coordination of protrusive forces in immune cell migration ,” Institute of Science and Technology Austria, 2025.","ama":"Dos Reis Rodrigues P. Coordination of protrusive forces in immune cell migration . 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20149\">10.15479/AT-ISTA-20149</a>"},"has_accepted_license":"1","acknowledgement":"I would like to acknowledge the\r\nfinancial support of the European Research Council through the ERC-SyG grant “Pushing from\r\nwithin: Control of cell shape, integrity and motility by cytoskeletal pushing forces”\r\n(01071793), which made this research possible. ","oa_version":"Published Version","title":"Coordination of protrusive forces in immune cell migration ","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"10703"},{"relation":"part_of_dissertation","status":"public","id":"20082"}]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"NanoFab"}],"language":[{"iso":"eng"}],"oa":1,"supervisor":[{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"}],"author":[{"first_name":"Patricia","orcid":"0000-0003-1681-508X","full_name":"Dos Reis Rodrigues, Patricia","last_name":"Dos Reis Rodrigues","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F"}],"article_processing_charge":"No","_id":"20149","corr_author":"1","month":"08","publication_identifier":{"issn":["2663-337X"]},"publisher":"Institute of Science and Technology Austria","type":"dissertation","day":"08","page":"114","status":"public","OA_place":"publisher","file":[{"file_name":"2025_ReisRodrigues_Patricia_Thesis.pdf","file_size":63885565,"date_updated":"2025-08-27T12:59:10Z","access_level":"open_access","creator":"prodrigu","content_type":"application/pdf","success":1,"date_created":"2025-08-27T12:59:10Z","relation":"main_file","checksum":"fda8a1070667c3562263f4867609b41b","file_id":"20232"},{"date_updated":"2025-08-27T13:02:28Z","file_name":"2025_ReisRodrigues_Patricia_Thesis.docx","file_size":50483434,"access_level":"closed","creator":"prodrigu","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2025-08-27T13:00:30Z","relation":"source_file","checksum":"e8b65affcbce846a926454df4b2867b9","file_id":"20233"}],"file_date_updated":"2025-08-27T13:02:28Z","abstract":[{"lang":"eng","text":"Immune responses depend on the coordinated and efficient migration of leukocytes. These\r\ncells, which are embedded and tightly confined within tissues, must navigate and traverse\r\ndiverse and complex three-dimensional environments. Leukocytes adapt their locomotory\r\nbehavior to the mechanical, geometrical, and biochemical characteristics of their\r\nsurroundings. In low-density environments, where the pore size of the interstitial matrix\r\nallows free passage, these cells position the nucleus directly behind the lamellipodium, the\r\nprotrusive actin structure that forms the leading front of the cell. In this configuration, they\r\nuse the nucleus as a gauge to identify the path of least resistance.\r\nHere, we show that in high-density environments, where the pore size precludes free passage\r\nof the cell body, leukocytes reposition the microtubule-organizing center (MTOC) and\r\nassociated organelles in front of the nucleus. In this configuration, they use actin structures\r\nprotruding orthogonally to the direction of migration in order to open a path for the cell body.\r\nWe identify two distinct actin populations that serve this purpose at different subcellular\r\nlocalizations. At the leading edge, local indentation of the plasma membrane leads to\r\nrecruitment of the Wiskott-Aldrich syndrome protein (WASp), which, via Arp2/3, results in\r\nthe formation of individual actin foci. At the cell body, actin polymerization is triggered by\r\nDOCK8, a Cdc42 exchange factor, resulting in the formation of a central actin pool.\r\nWe demonstrate that the central and peripheral actin pools are functionally communicating\r\nand that depletion of the central actin pool leads to increased actin accumulation at the cell\r\nfront, resulting in excessive extension of the leading edge."}],"alternative_title":["ISTA Thesis"],"degree_awarded":"PhD","doi":"10.15479/AT-ISTA-20149","publication_status":"published","department":[{"_id":"GradSch"},{"_id":"MiSi"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"date_updated":"2026-04-28T13:26:50Z","project":[{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793"}],"ddc":["570"],"date_published":"2025-08-08T00:00:00Z","year":"2025","date_created":"2025-08-08T09:18:02Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd"},{"file":[{"file_id":"20873","checksum":"90942491b499f70b0bf48b8aec2e7387","relation":"main_file","date_created":"2025-12-29T13:47:27Z","content_type":"application/pdf","success":1,"creator":"dernst","access_level":"open_access","file_name":"2025_TrendsNeurosciences_Hetzer.pdf","date_updated":"2025-12-29T13:47:27Z","file_size":327847}],"publication":"Trends in Neurosciences","intvolume":"        48","file_date_updated":"2025-12-29T13:47:27Z","abstract":[{"text":"In long-lived mammals, including humans, brain cell homeostasis is critical for maintaining brain function throughout life. Most neurons are generated during development and must maintain their cellular identity and plasticity to preserve brain function. Although extensive studies indicate the importance of recycling and regenerating cellular molecules to maintain cellular homeostasis, recent evidence has shown that some proteins and RNAs do not turn over for months and even years. We propose that these long-lived cellular molecules may be the basis for maintaining brain function in the long term, but also a potential convergent target of brain aging. We highlight key discoveries and challenges, and propose potential directions to unravel the mystery of brain cell longevity.","lang":"eng"}],"doi":"10.1016/j.tins.2025.07.004","publication_status":"published","external_id":{"pmid":["40744775"],"isi":["001568965400001"]},"department":[{"_id":"MaHe"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"volume":48,"date_updated":"2025-12-29T13:47:58Z","ddc":["570"],"date_published":"2025-09-01T00:00:00Z","year":"2025","date_created":"2025-08-10T22:01:29Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ieee":"M. Hetzer and T. Toda, “Long-lived cellular molecules in the brain,” <i>Trends in Neurosciences</i>, vol. 48, no. 9. Elsevier, pp. 645–654, 2025.","ama":"Hetzer M, Toda T. Long-lived cellular molecules in the brain. <i>Trends in Neurosciences</i>. 2025;48(9):645-654. doi:<a href=\"https://doi.org/10.1016/j.tins.2025.07.004\">10.1016/j.tins.2025.07.004</a>","short":"M. Hetzer, T. Toda, Trends in Neurosciences 48 (2025) 645–654.","apa":"Hetzer, M., &#38; Toda, T. (2025). Long-lived cellular molecules in the brain. <i>Trends in Neurosciences</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.tins.2025.07.004\">https://doi.org/10.1016/j.tins.2025.07.004</a>","ista":"Hetzer M, Toda T. 2025. Long-lived cellular molecules in the brain. Trends in Neurosciences. 48(9), 645–654.","chicago":"Hetzer, Martin, and Tomohisa Toda. “Long-Lived Cellular Molecules in the Brain.” <i>Trends in Neurosciences</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.tins.2025.07.004\">https://doi.org/10.1016/j.tins.2025.07.004</a>.","mla":"Hetzer, Martin, and Tomohisa Toda. “Long-Lived Cellular Molecules in the Brain.” <i>Trends in Neurosciences</i>, vol. 48, no. 9, Elsevier, 2025, pp. 645–54, doi:<a href=\"https://doi.org/10.1016/j.tins.2025.07.004\">10.1016/j.tins.2025.07.004</a>."},"has_accepted_license":"1","pmid":1,"acknowledgement":"The work was supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) (470322152 – T1347/3-1; 497658532 – T1347/4-1; 507965872 – T1347/5-1; and 460333672 – CRC1540 Exploring Brain Mechanics) to T.T., the Schram Foundation (T.T.), the European Research Council (ERC-2018-STG, 804468 EAGER; ERC-2023-COG, 101125034 NEUTIME) to T.T., the Hans-Georg Geis und Xue Hong Dong-Geis Foundation and Forschungsstiftung Medizin am Universitätsklinikum Erlangen to T.T., and the Interdisciplinary Centre for Clinical Research Erlangen (Interdisziplinäres Zentrum für Klinische Forschung, Universitätsklinikum Erlangen; P162 to T.T.). We thank Dr Laura J. Harrison for editing assistance.","scopus_import":"1","oa_version":"Published Version","isi":1,"OA_type":"hybrid","title":"Long-lived cellular molecules in the brain","oa":1,"language":[{"iso":"eng"}],"author":[{"first_name":"Martin W","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","full_name":"Hetzer, Martin W","orcid":"0000-0002-2111-992X","last_name":"Hetzer"},{"last_name":"Toda","full_name":"Toda, Tomohisa","first_name":"Tomohisa"}],"article_type":"original","article_processing_charge":"Yes (in subscription journal)","_id":"20154","issue":"9","corr_author":"1","PlanS_conform":"1","quality_controlled":"1","month":"09","publication_identifier":{"issn":["0166-2236"],"eissn":["1878-108X"]},"publisher":"Elsevier","type":"journal_article","day":"01","status":"public","page":"645-654","OA_place":"publisher"},{"acknowledgement":"The first author was funded by the European Union's Horizon 2020 research andinnovation program under the Marie Sklodowska-Curie grant agreements 754362 and 101034413,and partially by Project EFI (ANR-17-CE40-0030) of the French National Research Agency (ANR).The work of the second author was partially funded by the European Research Council (ERC) underthe European Union's Horizon 2020 research and innovation programme (grant agreement 810367),and by the Agence Nationale de la Recherche under grants ANR-19-CE40-0010 (QuAMProcs) andANR-21-CE40-0006 (SINEQ).","scopus_import":"1","oa_version":"Preprint","OA_type":"green","isi":1,"title":"How to construct explicit decay rates for kinetic Fokker–Planck equations?","arxiv":1,"citation":{"ista":"Brigati G, Stoltz G. 2025. How to construct explicit decay rates for kinetic Fokker–Planck equations? SIAM Journal on Mathematical Analysis. 57(4), 3587–3622.","apa":"Brigati, G., &#38; Stoltz, G. (2025). How to construct explicit decay rates for kinetic Fokker–Planck equations? <i>SIAM Journal on Mathematical Analysis</i>. Society for Industrial and Applied Mathematics. <a href=\"https://doi.org/10.1137/24M1700351\">https://doi.org/10.1137/24M1700351</a>","short":"G. Brigati, G. Stoltz, SIAM Journal on Mathematical Analysis 57 (2025) 3587–3622.","ama":"Brigati G, Stoltz G. How to construct explicit decay rates for kinetic Fokker–Planck equations? <i>SIAM Journal on Mathematical Analysis</i>. 2025;57(4):3587-3622. doi:<a href=\"https://doi.org/10.1137/24M1700351\">10.1137/24M1700351</a>","ieee":"G. Brigati and G. Stoltz, “How to construct explicit decay rates for kinetic Fokker–Planck equations?,” <i>SIAM Journal on Mathematical Analysis</i>, vol. 57, no. 4. Society for Industrial and Applied Mathematics, pp. 3587–3622, 2025.","mla":"Brigati, Giovanni, and Gabriel Stoltz. “How to Construct Explicit Decay Rates for Kinetic Fokker–Planck Equations?” <i>SIAM Journal on Mathematical Analysis</i>, vol. 57, no. 4, Society for Industrial and Applied Mathematics, 2025, pp. 3587–622, doi:<a href=\"https://doi.org/10.1137/24M1700351\">10.1137/24M1700351</a>.","chicago":"Brigati, Giovanni, and Gabriel Stoltz. “How to Construct Explicit Decay Rates for Kinetic Fokker–Planck Equations?” <i>SIAM Journal on Mathematical Analysis</i>. Society for Industrial and Applied Mathematics, 2025. <a href=\"https://doi.org/10.1137/24M1700351\">https://doi.org/10.1137/24M1700351</a>."},"month":"08","quality_controlled":"1","publisher":"Society for Industrial and Applied Mathematics","publication_identifier":{"eissn":["1095-7154"],"issn":["0036-1410"]},"day":"01","type":"journal_article","OA_place":"repository","status":"public","page":"3587-3622","oa":1,"language":[{"iso":"eng"}],"author":[{"last_name":"Brigati","full_name":"Brigati, Giovanni","id":"63ff57e8-1fbb-11ee-88f2-f558ffc59cf1","first_name":"Giovanni"},{"last_name":"Stoltz","full_name":"Stoltz, Gabriel","first_name":"Gabriel"}],"article_processing_charge":"No","article_type":"original","corr_author":"1","_id":"20155","issue":"4","abstract":[{"text":"We study time averages for the norm of solutions to kinetic Fokker–Planck equations associated with general Hamiltonians. We provide fully explicit and constructive decay estimates for systems subject to a confining potential, allowing fat-tail, subexponential and (super-)exponential local equilibria, which also include the classic Maxwellian case. The key step in our estimates is a modified Poincaré inequality, obtained via a Lions–Poincaré inequality and an averaging lemma.","lang":"eng"}],"ec_funded":1,"doi":"10.1137/24M1700351","publication_status":"published","department":[{"_id":"JaMa"}],"external_id":{"arxiv":["2302.14506"],"isi":["001550830900006"]},"publication":"SIAM Journal on Mathematical Analysis","intvolume":"        57","year":"2025","date_created":"2025-08-10T22:01:29Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.48550/arXiv.2302.14506"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2025-11-05T13:51:40Z","volume":57,"date_published":"2025-08-01T00:00:00Z","project":[{"call_identifier":"H2020","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413","name":"IST-BRIDGE: International postdoctoral program"}]},{"has_accepted_license":"1","citation":{"ama":"Schön H. The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20167\">10.15479/AT-ISTA-20167</a>","short":"H. Schön, The ER Complex SUTU-7/MACO-1 Regulates the Fate of MRNAs Encoding GPCRs, Institute of Science and Technology Austria, 2025.","ieee":"H. Schön, “The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs,” Institute of Science and Technology Austria, 2025.","apa":"Schön, H. (2025). <i>The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20167\">https://doi.org/10.15479/AT-ISTA-20167</a>","ista":"Schön H. 2025. The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs. Institute of Science and Technology Austria.","chicago":"Schön, Hanna. “The ER Complex SUTU-7/MACO-1 Regulates the Fate of MRNAs Encoding GPCRs.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20167\">https://doi.org/10.15479/AT-ISTA-20167</a>.","mla":"Schön, Hanna. <i>The ER Complex SUTU-7/MACO-1 Regulates the Fate of MRNAs Encoding GPCRs</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20167\">10.15479/AT-ISTA-20167</a>."},"oa_version":"Published Version","acknowledgement":"This work was supported by EMBO (ALTF 302-2019 to Niko Amin-Wetzel), the FWF\r\n(ESPRIT PR1054E140 to Niko Amin-Wetzel), the European Research Council\r\n(Advanced Grant 269058 to Mario de Bono) and Wellcome (209504/A/17/Z\r\nInvestigator Award to Mario de Bono). ","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"title":"The ER complex SUTU-7/MACO-1 regulates the fate of mRNAs encoding GPCRs","supervisor":[{"id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8347-0443","full_name":"de Bono, Mario","last_name":"de Bono","first_name":"Mario"}],"author":[{"first_name":"Hanna","full_name":"Schön, Hanna","last_name":"Schön","id":"C8E17EDC-D7AA-11E9-B7B7-45ECE5697425"}],"language":[{"iso":"eng"}],"_id":"20167","corr_author":"1","article_processing_charge":"No","publisher":"Institute of Science and Technology Austria","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-061-9"]},"month":"08","OA_place":"publisher","page":"171","status":"public","day":"13","type":"dissertation","file":[{"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2025-09-08T14:33:50Z","relation":"source_file","checksum":"b40c74404b8d9593802dabf57bfdf10f","file_id":"20311","file_size":78812587,"date_updated":"2025-09-09T08:57:04Z","file_name":"2025_Schoen_Hanna_Thesis.docx","access_level":"closed","creator":"hschoen"},{"access_level":"closed","embargo_to":"open_access","file_size":9667057,"date_updated":"2025-09-18T14:12:29Z","file_name":"2025_Schoen_Hanna_Thesis.pdf","embargo":"2026-09-15","creator":"hschoen","date_created":"2025-09-11T14:20:59Z","content_type":"application/pdf","file_id":"20347","checksum":"16abc3ff66396ce2457fe07ffa8bed90","relation":"main_file"}],"file_date_updated":"2025-09-18T14:12:29Z","degree_awarded":"PhD","doi":"10.15479/AT-ISTA-20167","alternative_title":["ISTA Thesis"],"department":[{"_id":"GradSch"},{"_id":"MaDe"}],"publication_status":"published","date_updated":"2026-04-07T11:50:26Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"date_published":"2025-08-13T00:00:00Z","ddc":["570"],"project":[{"_id":"23870BE8-32DE-11EA-91FC-C7463DDC885E","grant_number":"209504/A/17/Z","name":"Molecular mechanisms of neural circuit function"},{"_id":"23813290-32DE-11EA-91FC-C7463DDC885E","grant_number":"ALTF 302-2019","name":"Control of gene expression at the endoplasmic reticulum"}],"date_created":"2025-08-13T11:13:13Z","year":"2025","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd"},{"scopus_import":"1","acknowledgement":"This work was supported by an Austrian Research Fund (FWF) grant to B.V. (PAT 8748323) and by the Louisiana Board of Regents Research Competitiveness Subprogram (LEQSF(2025-28)-RD-A-20) to MAT.","oa_version":"Published Version","OA_type":"hybrid","isi":1,"title":"Insect sex chromosome evolution: Conservation, turnover, and mechanisms of dosage compensation","citation":{"ista":"Toups MA, Vicoso B. 2025. Insect sex chromosome evolution: Conservation, turnover, and mechanisms of dosage compensation. Current Opinion in Insect Science. 72, 101411.","apa":"Toups, M. A., &#38; Vicoso, B. (2025). Insect sex chromosome evolution: Conservation, turnover, and mechanisms of dosage compensation. <i>Current Opinion in Insect Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cois.2025.101411\">https://doi.org/10.1016/j.cois.2025.101411</a>","short":"M.A. Toups, B. Vicoso, Current Opinion in Insect Science 72 (2025).","ama":"Toups MA, Vicoso B. Insect sex chromosome evolution: Conservation, turnover, and mechanisms of dosage compensation. <i>Current Opinion in Insect Science</i>. 2025;72. doi:<a href=\"https://doi.org/10.1016/j.cois.2025.101411\">10.1016/j.cois.2025.101411</a>","ieee":"M. A. Toups and B. Vicoso, “Insect sex chromosome evolution: Conservation, turnover, and mechanisms of dosage compensation,” <i>Current Opinion in Insect Science</i>, vol. 72. Elsevier, 2025.","mla":"Toups, Melissa A., and Beatriz Vicoso. “Insect Sex Chromosome Evolution: Conservation, Turnover, and Mechanisms of Dosage Compensation.” <i>Current Opinion in Insect Science</i>, vol. 72, 101411, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.cois.2025.101411\">10.1016/j.cois.2025.101411</a>.","chicago":"Toups, Melissa A, and Beatriz Vicoso. “Insect Sex Chromosome Evolution: Conservation, Turnover, and Mechanisms of Dosage Compensation.” <i>Current Opinion in Insect Science</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.cois.2025.101411\">https://doi.org/10.1016/j.cois.2025.101411</a>."},"has_accepted_license":"1","month":"12","quality_controlled":"1","article_number":"101411","publisher":"Elsevier","publication_identifier":{"eissn":["2214-5753"],"issn":["2214-5745"]},"type":"journal_article","day":"01","status":"public","OA_place":"publisher","language":[{"iso":"eng"}],"oa":1,"author":[{"id":"4E099E4E-F248-11E8-B48F-1D18A9856A87","last_name":"Toups","orcid":"0000-0002-9752-7380","full_name":"Toups, Melissa A","first_name":"Melissa A"},{"last_name":"Vicoso","full_name":"Vicoso, Beatriz","orcid":"0000-0002-4579-8306","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","first_name":"Beatriz"}],"article_type":"review","article_processing_charge":"Yes (via OA deal)","corr_author":"1","_id":"20182","PlanS_conform":"1","abstract":[{"lang":"eng","text":"Sex chromosomes have evolved many times throughout the tree of life, and understanding what has shaped their unusual morphological, sequence, and regulatory features has been a long-standing goal. Most early insights into insect sex chromosome biology came from a few model species, such as the fruit fly Drosophila melanogaster, which limited broad-scale evolutionary inferences. More recently, extensive comparative genomics studies have uncovered several unexpected patterns, which we highlight in this review. First, we describe the conservation of the ancestral X chromosome over 450 million years but also its recurrent turnover (i.e. its reversal to an autosome when a new X chromosome arose) in at least one order. We then summarize classical and more recent findings on how insects modulate the expression of X-linked genes following the degradation of the Y chromosome and how the diverse mechanisms of dosage compensation identified may elucidate important principles of sex chromosome regulatory evolution."}],"doi":"10.1016/j.cois.2025.101411","publication_status":"published","external_id":{"isi":["001582424100001"]},"department":[{"_id":"BeVi"}],"file":[{"file_size":897079,"date_updated":"2025-12-30T13:14:20Z","file_name":"2025_CurrOpinionInsectScience_Toups.pdf","access_level":"open_access","creator":"dernst","success":1,"content_type":"application/pdf","date_created":"2025-12-30T13:14:20Z","relation":"main_file","file_id":"20917","checksum":"262640abc34277686b56eb60102976f6"}],"intvolume":"        72","publication":"Current Opinion in Insect Science","file_date_updated":"2025-12-30T13:14:20Z","year":"2025","date_created":"2025-08-17T22:01:35Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":72,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"date_updated":"2025-12-30T13:14:38Z","ddc":["570"],"project":[{"grant_number":"PAT 8748323","_id":"8ed82125-16d5-11f0-9cad-fbcae312235b","name":"Sex chromosomes in evolution and development"}],"date_published":"2025-12-01T00:00:00Z"}]