---
_id: '2976'
abstract:
- lang: eng
text: |-
Side channel attacks on cryptographic systems exploit information
gained from physical implementations rather than theoretical
weaknesses of a scheme. In recent years, major achievements were made
for the class of so called access-driven cache attacks. Such attacks
exploit the leakage of the memory locations accessed by a victim
process.
In this paper we consider the AES block cipher and present an attack
which is capable of recovering the full secret key in almost realtime
for AES-128, requiring only a very limited number of observed
encryptions. Unlike previous attacks, we do not require any
information about the plaintext (such as its distribution, etc.).
Moreover, for the first time, we also show how the plaintext can be
recovered without having access to the ciphertext at all. It is the
first working attack on AES implementations using compressed
tables. There, no efficient techniques to identify the beginning
of AES rounds is known, which is the fundamental assumption underlying previous
attacks.
We have a fully working implementation of our attack which is able to
recover AES keys after observing as little as 100 encryptions. It
works against the OpenSSL 0.9.8n implementation of AES on Linux
systems. Our spy process does not require any special privileges
beyond those of a standard Linux user. A contribution of probably
independent interest is a denial of service attack on the task scheduler of
current Linux systems (CFS), which allows one to observe (on average)
every single memory access of a victim process.
acknowledgement: |-
This work was in part funded by the European Community’s Seventh Framework Programme (FP7) under grant agreement no. 216499 and the Swiss Hasler Foundation.
An extended abstract was also accepted for COSADE 2011.
author:
- first_name: David
full_name: Gullasch, David
last_name: Gullasch
- first_name: Endre
full_name: Bangerter, Endre
last_name: Bangerter
- first_name: Stephan
full_name: Stephan Krenn
id: 329FCCF0-F248-11E8-B48F-1D18A9856A87
last_name: Krenn
orcid: 0000-0003-2835-9093
citation:
ama: 'Gullasch D, Bangerter E, Krenn S. Cache Games - Bringing Access-Based Cache
Attacks on AES to Practice. In: IEEE; 2011:490-505. doi:10.1109/SP.2011.22'
apa: 'Gullasch, D., Bangerter, E., & Krenn, S. (2011). Cache Games - Bringing
Access-Based Cache Attacks on AES to Practice (pp. 490–505). Presented at the
S&P: IEEE Symposium on Security and Privacy, IEEE. https://doi.org/10.1109/SP.2011.22'
chicago: Gullasch, David, Endre Bangerter, and Stephan Krenn. “Cache Games - Bringing
Access-Based Cache Attacks on AES to Practice,” 490–505. IEEE, 2011. https://doi.org/10.1109/SP.2011.22.
ieee: 'D. Gullasch, E. Bangerter, and S. Krenn, “Cache Games - Bringing Access-Based
Cache Attacks on AES to Practice,” presented at the S&P: IEEE Symposium on
Security and Privacy, 2011, pp. 490–505.'
ista: 'Gullasch D, Bangerter E, Krenn S. 2011. Cache Games - Bringing Access-Based
Cache Attacks on AES to Practice. S&P: IEEE Symposium on Security and Privacy,
490–505.'
mla: Gullasch, David, et al. Cache Games - Bringing Access-Based Cache Attacks
on AES to Practice. IEEE, 2011, pp. 490–505, doi:10.1109/SP.2011.22.
short: D. Gullasch, E. Bangerter, S. Krenn, in:, IEEE, 2011, pp. 490–505.
conference:
name: 'S&P: IEEE Symposium on Security and Privacy'
date_created: 2018-12-11T12:00:39Z
date_published: 2011-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:11Z
day: '01'
doi: 10.1109/SP.2011.22
extern: 1
main_file_link:
- open_access: '0'
url: http://eprint.iacr.org/2010/594.pdf
month: '01'
page: 490 - 505
publication_status: published
publisher: IEEE
publist_id: '3727'
quality_controlled: 0
status: public
title: Cache Games - Bringing Access-Based Cache Attacks on AES to Practice
type: conference
year: '2011'
...
---
_id: '2977'
abstract:
- lang: eng
text: "Cryptographic two-party protocols are used ubiquitously in\n everyday
life. While some of these protocols are easy to\n understand and implement
(e.g., key exchange or transmission of\n encrypted data), many of them are
much more complex (e.g.,\n e-banking and e-voting applications, or anonymous
authentication\n and credential systems).\n\n For a software engineer without
appropriate cryptographic skills\n the implementation of such protocols is
often difficult, time\n consuming and error-prone. For this reason, a number
of compilers\n supporting programmers have been published in recent\n years.
However, they are either designed for very specific\n cryptographic primitives
(e.g., zero-knowledge proofs of\n knowledge), or they only offer a very low
level of abstraction and\n thus again demand substantial mathematical and cryptographic\n
\ skills from the programmer. Finally, some of the existing\n compilers do
not produce executable code, but only metacode which\n has to be instantiated
with mathematical libraries, encryption\n routines, etc. before it can actually
be used.\n \n In this paper we present a cryptographically aware compiler
which\n is equally useful to cryptographers who want to benchmark\n protocols
designed on paper, and to programmers who want to\n implement complex security
sensitive protocols without having to\n understand all subtleties. Our tool
offers a high level of\n abstraction and outputs well-structured and documented
Java\n code. We believe that our compiler can contribute to shortening\n the
development cycles of cryptographic applications and to\n reducing their error-proneness."
acknowledgement: This work was in part funded by the European Community’s Seventh
Framework Programme (FP7) under grant agreement no. 216499 and the Swiss Hasler
Foundation under projects no. 09037 and 10069.
author:
- first_name: Endre
full_name: Bangerter, Endre
last_name: Bangerter
- first_name: Stephan
full_name: Stephan Krenn
id: 329FCCF0-F248-11E8-B48F-1D18A9856A87
last_name: Krenn
orcid: 0000-0003-2835-9093
- first_name: Martial
full_name: Seifriz, Martial
last_name: Seifriz
- first_name: Ulrich
full_name: Ultes-Nitsche, Ulrich
last_name: Ultes Nitsche
citation:
ama: 'Bangerter E, Krenn S, Seifriz M, Ultes Nitsche U. cPLC - A Cryptographic Programming
Language and Compiler. In: Venter H, Coetzee M, Loock M, eds. IEEE; 2011. doi:10.1109/ISSA.2011.6027533'
apa: 'Bangerter, E., Krenn, S., Seifriz, M., & Ultes Nitsche, U. (2011). cPLC
- A Cryptographic Programming Language and Compiler. In H. Venter, M. Coetzee,
& M. Loock (Eds.). Presented at the ISSA: Information Security South Africa,
IEEE. https://doi.org/10.1109/ISSA.2011.6027533'
chicago: Bangerter, Endre, Stephan Krenn, Martial Seifriz, and Ulrich Ultes Nitsche.
“CPLC - A Cryptographic Programming Language and Compiler.” edited by Hein Venter,
Marijke Coetzee, and Marianne Loock. IEEE, 2011. https://doi.org/10.1109/ISSA.2011.6027533.
ieee: 'E. Bangerter, S. Krenn, M. Seifriz, and U. Ultes Nitsche, “cPLC - A Cryptographic
Programming Language and Compiler,” presented at the ISSA: Information Security
South Africa, 2011.'
ista: 'Bangerter E, Krenn S, Seifriz M, Ultes Nitsche U. 2011. cPLC - A Cryptographic
Programming Language and Compiler. ISSA: Information Security South Africa.'
mla: Bangerter, Endre, et al. CPLC - A Cryptographic Programming Language and
Compiler. Edited by Hein Venter et al., IEEE, 2011, doi:10.1109/ISSA.2011.6027533.
short: E. Bangerter, S. Krenn, M. Seifriz, U. Ultes Nitsche, in:, H. Venter, M.
Coetzee, M. Loock (Eds.), IEEE, 2011.
conference:
name: 'ISSA: Information Security South Africa'
date_created: 2018-12-11T12:00:39Z
date_published: 2011-08-01T00:00:00Z
date_updated: 2021-01-12T07:40:12Z
day: '01'
doi: 10.1109/ISSA.2011.6027533
editor:
- first_name: Hein
full_name: Venter, Hein S.
last_name: Venter
- first_name: Marijke
full_name: Coetzee, Marijke
last_name: Coetzee
- first_name: Marianne
full_name: Loock, Marianne
last_name: Loock
extern: 1
month: '08'
publication_status: published
publisher: IEEE
publist_id: '3726'
quality_controlled: 0
status: public
title: cPLC - A Cryptographic Programming Language and Compiler
type: conference
year: '2011'
...
---
_id: '3082'
abstract:
- lang: eng
text: Shoot branching is one of the major determinants of plant architecture. Polar
auxin transport in stems is necessary for the control of bud outgrowth by a dominant
apex. Here, we show that following decapitation in pea (Pisum sativum L.), the
axillary buds establish directional auxin export by subcellular polarization of
PIN auxin transporters. Apical auxin application on the decapitated stem prevents
this PIN polarization and canalization of laterally applied auxin. These results
support a model in which the apical and lateral auxin sources compete for primary
channels of auxin transport in the stem to control the outgrowth of axillary buds.
author:
- first_name: Jozef
full_name: Balla, Jozef
last_name: Balla
- first_name: Petr
full_name: Kalousek, Petr
last_name: Kalousek
- first_name: Vilém
full_name: Reinöhl, Vilém
last_name: Reinöhl
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Stanislav
full_name: Procházka, Stanislav
last_name: Procházka
citation:
ama: Balla J, Kalousek P, Reinöhl V, Friml J, Procházka S. Competitive canalization
of PIN dependent auxin flow from axillary buds controls pea bud outgrowth. Plant
Journal. 2011;65(4):571-577. doi:10.1111/j.1365-313X.2010.04443.x
apa: Balla, J., Kalousek, P., Reinöhl, V., Friml, J., & Procházka, S. (2011).
Competitive canalization of PIN dependent auxin flow from axillary buds controls
pea bud outgrowth. Plant Journal. Wiley-Blackwell. https://doi.org/10.1111/j.1365-313X.2010.04443.x
chicago: Balla, Jozef, Petr Kalousek, Vilém Reinöhl, Jiří Friml, and Stanislav Procházka.
“Competitive Canalization of PIN Dependent Auxin Flow from Axillary Buds Controls
Pea Bud Outgrowth.” Plant Journal. Wiley-Blackwell, 2011. https://doi.org/10.1111/j.1365-313X.2010.04443.x.
ieee: J. Balla, P. Kalousek, V. Reinöhl, J. Friml, and S. Procházka, “Competitive
canalization of PIN dependent auxin flow from axillary buds controls pea bud outgrowth,”
Plant Journal, vol. 65, no. 4. Wiley-Blackwell, pp. 571–577, 2011.
ista: Balla J, Kalousek P, Reinöhl V, Friml J, Procházka S. 2011. Competitive canalization
of PIN dependent auxin flow from axillary buds controls pea bud outgrowth. Plant
Journal. 65(4), 571–577.
mla: Balla, Jozef, et al. “Competitive Canalization of PIN Dependent Auxin Flow
from Axillary Buds Controls Pea Bud Outgrowth.” Plant Journal, vol. 65,
no. 4, Wiley-Blackwell, 2011, pp. 571–77, doi:10.1111/j.1365-313X.2010.04443.x.
short: J. Balla, P. Kalousek, V. Reinöhl, J. Friml, S. Procházka, Plant Journal
65 (2011) 571–577.
date_created: 2018-12-11T12:01:16Z
date_published: 2011-02-01T00:00:00Z
date_updated: 2021-01-12T07:40:56Z
day: '01'
doi: 10.1111/j.1365-313X.2010.04443.x
extern: 1
intvolume: ' 65'
issue: '4'
month: '02'
page: 571 - 577
publication: Plant Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3619'
quality_controlled: 0
status: public
title: Competitive canalization of PIN dependent auxin flow from axillary buds controls
pea bud outgrowth
type: journal_article
volume: 65
year: '2011'
...
---
_id: '3083'
author:
- first_name: David
full_name: Robinson, David G
last_name: Robinson
- first_name: David
full_name: Scheuring, David
last_name: Scheuring
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Robinson D, Scheuring D, Naramoto S, Friml J. ARF1 localizes to the golgi and
the trans Golgi network. Plant Cell. 2011;23(3):846-849. doi:10.1105/tpc.110.082099
apa: Robinson, D., Scheuring, D., Naramoto, S., & Friml, J. (2011). ARF1 localizes
to the golgi and the trans Golgi network. Plant Cell. American Society
of Plant Biologists. https://doi.org/10.1105/tpc.110.082099
chicago: Robinson, David, David Scheuring, Satoshi Naramoto, and Jiří Friml. “ARF1
Localizes to the Golgi and the Trans Golgi Network.” Plant Cell. American
Society of Plant Biologists, 2011. https://doi.org/10.1105/tpc.110.082099.
ieee: D. Robinson, D. Scheuring, S. Naramoto, and J. Friml, “ARF1 localizes to the
golgi and the trans Golgi network,” Plant Cell, vol. 23, no. 3. American
Society of Plant Biologists, pp. 846–849, 2011.
ista: Robinson D, Scheuring D, Naramoto S, Friml J. 2011. ARF1 localizes to the
golgi and the trans Golgi network. Plant Cell. 23(3), 846–849.
mla: Robinson, David, et al. “ARF1 Localizes to the Golgi and the Trans Golgi Network.”
Plant Cell, vol. 23, no. 3, American Society of Plant Biologists, 2011,
pp. 846–49, doi:10.1105/tpc.110.082099.
short: D. Robinson, D. Scheuring, S. Naramoto, J. Friml, Plant Cell 23 (2011) 846–849.
date_created: 2018-12-11T12:01:16Z
date_published: 2011-03-01T00:00:00Z
date_updated: 2021-01-12T07:40:56Z
day: '01'
doi: 10.1105/tpc.110.082099
extern: 1
intvolume: ' 23'
issue: '3'
month: '03'
page: 846 - 849
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3617'
quality_controlled: 0
status: public
title: ARF1 localizes to the golgi and the trans Golgi network
type: journal_article
volume: 23
year: '2011'
...
---
_id: '3084'
abstract:
- lang: eng
text: |2-
A central question in developmental biology concerns the mechanism of generation and maintenance of cell polarity, because these processes are essential for many cellular functions and multicellular development [1]. In plants, cell polarity has an additional role in mediating directional transport of the plant hormone auxin that is crucial for multiple developmental processes [2-4]. In addition, plant cells have a complex extracellular matrix, the cell wall [5, 6], whose role in regulating cellular processes, including cell polarity, is unexplored. We have found that polar distribution of PIN auxin transporters [7] in plant cells is maintained by connections between polar domains at the plasma membrane and the cell wall. Genetic and pharmacological interference with cellulose, the major component of the cell wall, or mechanical interference with the cell wall disrupts these connections and leads to increased lateral diffusion and loss of polar distribution of PIN transporters for the phytohormone auxin. Our results reveal a plant-specific mechanism for cell polarity maintenance and provide a conceptual framework for modulating cell polarity and plant development via endogenous and environmental manipulations of the cellulose-based extracellular matrix.
author:
- first_name: Elena
full_name: Feraru, Elena
last_name: Feraru
- first_name: Mugurel
full_name: Feraru, Mugurel I
last_name: Feraru
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Alexandre
full_name: Martinière, Alexandre
last_name: Martinière
- first_name: Grégory
full_name: Mouille, Grégory
last_name: Mouille
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Samantha
full_name: Vernhettes, Samantha
last_name: Vernhettes
- first_name: John
full_name: Runions, John
last_name: Runions
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Feraru E, Feraru M, Kleine Vehn J, et al. PIN polarity maintenance by the cell
wall in Arabidopsis. Current Biology. 2011;21(4):338-343. doi:10.1016/j.cub.2011.01.036
apa: Feraru, E., Feraru, M., Kleine Vehn, J., Martinière, A., Mouille, G., Vanneste,
S., … Friml, J. (2011). PIN polarity maintenance by the cell wall in Arabidopsis.
Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2011.01.036
chicago: Feraru, Elena, Mugurel Feraru, Jürgen Kleine Vehn, Alexandre Martinière,
Grégory Mouille, Steffen Vanneste, Samantha Vernhettes, John Runions, and Jiří
Friml. “PIN Polarity Maintenance by the Cell Wall in Arabidopsis.” Current
Biology. Cell Press, 2011. https://doi.org/10.1016/j.cub.2011.01.036.
ieee: E. Feraru et al., “PIN polarity maintenance by the cell wall in Arabidopsis,”
Current Biology, vol. 21, no. 4. Cell Press, pp. 338–343, 2011.
ista: Feraru E, Feraru M, Kleine Vehn J, Martinière A, Mouille G, Vanneste S, Vernhettes
S, Runions J, Friml J. 2011. PIN polarity maintenance by the cell wall in Arabidopsis.
Current Biology. 21(4), 338–343.
mla: Feraru, Elena, et al. “PIN Polarity Maintenance by the Cell Wall in Arabidopsis.”
Current Biology, vol. 21, no. 4, Cell Press, 2011, pp. 338–43, doi:10.1016/j.cub.2011.01.036.
short: E. Feraru, M. Feraru, J. Kleine Vehn, A. Martinière, G. Mouille, S. Vanneste,
S. Vernhettes, J. Runions, J. Friml, Current Biology 21 (2011) 338–343.
date_created: 2018-12-11T12:01:17Z
date_published: 2011-02-22T00:00:00Z
date_updated: 2021-01-12T07:40:56Z
day: '22'
doi: 10.1016/j.cub.2011.01.036
extern: 1
intvolume: ' 21'
issue: '4'
month: '02'
page: 338 - 343
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '3618'
quality_controlled: 0
status: public
title: PIN polarity maintenance by the cell wall in Arabidopsis
type: journal_article
volume: 21
year: '2011'
...
---
_id: '3085'
abstract:
- lang: eng
text: Phototropism is an adaptation response, through which plants grow towards
the light. It involves light perception and asymmetric distribution of the plant
hormone auxin. Here we identify a crucial part of the mechanism for phototropism,
revealing how light perception initiates auxin redistribution that leads to directional
growth. We show that light polarizes the cellular localization of the auxin efflux
carrier PIN3 in hypocotyl endodermis cells, resulting in changes in auxin distribution
and differential growth. In the dark, high expression and activity of the PINOID
(PID) kinase correlates with apolar targeting of PIN3 to all cell sides. Following
illumination, light represses PINOID transcription and PIN3 is polarized specifically
to the inner cell sides by GNOM ARF GTPase GEF (guanine nucleotide exchange factor)-dependent
trafficking. Thus, differential trafficking at the shaded and illuminated hypocotyl
side aligns PIN3 polarity with the light direction, and presumably redirects auxin
flow towards the shaded side, where auxin promotes growth, causing hypocotyls
to bend towards the light. Our results imply that PID phosphorylation-dependent
recruitment of PIN proteins into distinct trafficking pathways is a mechanism
to polarize auxin fluxes in response to different environmental and endogenous
cues.
author:
- first_name: Zhaojun
full_name: Ding, Zhaojun
last_name: Ding
- first_name: Carlos
full_name: Galván-Ampudia, Carlos S
last_name: Galván Ampudia
- first_name: Emilie
full_name: Demarsy, Emilie
last_name: Demarsy
- first_name: Łukasz
full_name: Łangowski, Łukasz
last_name: Łangowski
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Yuanwei
full_name: Fan, Yuanwei
last_name: Fan
- first_name: Miyo
full_name: Morita, Miyo T
last_name: Morita
- first_name: Masao
full_name: Tasaka, Masao
last_name: Tasaka
- first_name: Christian
full_name: Fankhauser, Christian
last_name: Fankhauser
- first_name: Remko
full_name: Offringa, Remko
last_name: Offringa
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Ding Z, Galván Ampudia C, Demarsy E, et al. Light-mediated polarization of
the PIN3 auxin transporter for the phototropic response in Arabidopsis. Nature
Cell Biology. 2011;13(4):447-453. doi:10.1038/ncb2208
apa: Ding, Z., Galván Ampudia, C., Demarsy, E., Łangowski, Ł., Kleine Vehn, J.,
Fan, Y., … Friml, J. (2011). Light-mediated polarization of the PIN3 auxin transporter
for the phototropic response in Arabidopsis. Nature Cell Biology. Nature
Publishing Group. https://doi.org/10.1038/ncb2208
chicago: Ding, Zhaojun, Carlos Galván Ampudia, Emilie Demarsy, Łukasz Łangowski,
Jürgen Kleine Vehn, Yuanwei Fan, Miyo Morita, et al. “Light-Mediated Polarization
of the PIN3 Auxin Transporter for the Phototropic Response in Arabidopsis.” Nature
Cell Biology. Nature Publishing Group, 2011. https://doi.org/10.1038/ncb2208.
ieee: Z. Ding et al., “Light-mediated polarization of the PIN3 auxin transporter
for the phototropic response in Arabidopsis,” Nature Cell Biology, vol.
13, no. 4. Nature Publishing Group, pp. 447–453, 2011.
ista: Ding Z, Galván Ampudia C, Demarsy E, Łangowski Ł, Kleine Vehn J, Fan Y, Morita
M, Tasaka M, Fankhauser C, Offringa R, Friml J. 2011. Light-mediated polarization
of the PIN3 auxin transporter for the phototropic response in Arabidopsis. Nature
Cell Biology. 13(4), 447–453.
mla: Ding, Zhaojun, et al. “Light-Mediated Polarization of the PIN3 Auxin Transporter
for the Phototropic Response in Arabidopsis.” Nature Cell Biology, vol.
13, no. 4, Nature Publishing Group, 2011, pp. 447–53, doi:10.1038/ncb2208.
short: Z. Ding, C. Galván Ampudia, E. Demarsy, Ł. Łangowski, J. Kleine Vehn, Y.
Fan, M. Morita, M. Tasaka, C. Fankhauser, R. Offringa, J. Friml, Nature Cell Biology
13 (2011) 447–453.
date_created: 2018-12-11T12:01:17Z
date_published: 2011-04-01T00:00:00Z
date_updated: 2021-01-12T07:40:57Z
day: '01'
doi: 10.1038/ncb2208
extern: 1
intvolume: ' 13'
issue: '4'
month: '04'
page: 447 - 453
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3616'
quality_controlled: 0
status: public
title: Light-mediated polarization of the PIN3 auxin transporter for the phototropic
response in Arabidopsis
type: journal_article
volume: 13
year: '2011'
...
---
_id: '3086'
abstract:
- lang: eng
text: PIN-FORMED (PIN)-dependent auxin transport is essential for plant development
and its modulation in response to the environment or endogenous signals. A NON-PHOTOTROPIC
HYPOCOTYL 3 (NPH3)-like protein, MACCHI-BOU 4 (MAB4), has been shown to control
PIN1 localization during organ formation, but its contribution is limited. The
Arabidopsis genome contains four genes, MAB4/ENP/NPY1-LIKE1 (MEL1), MEL2, MEL3
and MEL4, highly homologous to MAB4. Genetic analysis disclosed functional redundancy
between MAB4 and MEL genes in regulation of not only organ formation but also
of root gravitropism, revealing that NPH3 family proteins have a wider range of
functions than previously suspected. Multiple mutants showed severe reduction
in PIN abundance and PIN polar localization, leading to defective expression of
an auxin responsive marker DR5rev::GFP. Pharmacological analyses and fluorescence
recovery after photo-bleaching experiments showed that mel mutations increase
PIN2 internalization from the plasma membrane, but affect neither intracellular
PIN2 trafficking nor PIN2 lateral diffusion at the plasma membrane. Notably, all
MAB4 subfamily proteins show polar localization at the cell periphery in plants.
The MAB4 polarity was almost identical to PIN polarity. Our results suggest that
the MAB4 subfamily proteins specifically retain PIN proteins in a polarized manner
at the plasma membrane, thus controlling directional auxin transport and plant
development.
author:
- first_name: Masahiko
full_name: Furutani, Masahiko
last_name: Furutani
- first_name: Norihito
full_name: Sakamoto, Norihito
last_name: Sakamoto
- first_name: Shuhei
full_name: Yoshida, Shuhei
last_name: Yoshida
- first_name: Takahito
full_name: Kajiwara, Takahito
last_name: Kajiwara
- first_name: Hélène
full_name: Robert, Hélène S
last_name: Robert
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Masao
full_name: Tasaka, Masao
last_name: Tasaka
citation:
ama: Furutani M, Sakamoto N, Yoshida S, et al. Polar localized NPH3-like proteins
regulate polarity and endocytosis of PIN-FORMED auxin efflux carriers. Development.
2011;138(10):2069-2078. doi:10.1242/dev.057745
apa: Furutani, M., Sakamoto, N., Yoshida, S., Kajiwara, T., Robert, H., Friml, J.,
& Tasaka, M. (2011). Polar localized NPH3-like proteins regulate polarity
and endocytosis of PIN-FORMED auxin efflux carriers. Development. Company
of Biologists. https://doi.org/10.1242/dev.057745
chicago: Furutani, Masahiko, Norihito Sakamoto, Shuhei Yoshida, Takahito Kajiwara,
Hélène Robert, Jiří Friml, and Masao Tasaka. “Polar Localized NPH3-like Proteins
Regulate Polarity and Endocytosis of PIN-FORMED Auxin Efflux Carriers.” Development.
Company of Biologists, 2011. https://doi.org/10.1242/dev.057745.
ieee: M. Furutani et al., “Polar localized NPH3-like proteins regulate polarity
and endocytosis of PIN-FORMED auxin efflux carriers,” Development, vol.
138, no. 10. Company of Biologists, pp. 2069–2078, 2011.
ista: Furutani M, Sakamoto N, Yoshida S, Kajiwara T, Robert H, Friml J, Tasaka M.
2011. Polar localized NPH3-like proteins regulate polarity and endocytosis of
PIN-FORMED auxin efflux carriers. Development. 138(10), 2069–2078.
mla: Furutani, Masahiko, et al. “Polar Localized NPH3-like Proteins Regulate Polarity
and Endocytosis of PIN-FORMED Auxin Efflux Carriers.” Development, vol.
138, no. 10, Company of Biologists, 2011, pp. 2069–78, doi:10.1242/dev.057745.
short: M. Furutani, N. Sakamoto, S. Yoshida, T. Kajiwara, H. Robert, J. Friml, M.
Tasaka, Development 138 (2011) 2069–2078.
date_created: 2018-12-11T12:01:17Z
date_published: 2011-05-01T00:00:00Z
date_updated: 2021-01-12T07:40:57Z
day: '01'
doi: 10.1242/dev.057745
extern: 1
intvolume: ' 138'
issue: '10'
month: '05'
page: 2069 - 2078
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '3615'
quality_controlled: 0
status: public
title: Polar localized NPH3-like proteins regulate polarity and endocytosis of PIN-FORMED
auxin efflux carriers
type: journal_article
volume: 138
year: '2011'
...
---
_id: '3087'
abstract:
- lang: eng
text: Endocytosis is a crucial mechanism by which eukaryotic cells internalize extracellular
and plasma membrane material, and it is required for a multitude of cellular and
developmental processes in unicellular and multicellular organisms. In animals
and yeast, the best characterized pathway for endocytosis depends on the function
of the vesicle coat protein clathrin. Clathrinmediated endocytosis has recently
been demonstrated also in plant cells, but its physiological and developmental
roles remain unclear. Here, we assessed the roles of the clathrin-mediated mechanism
of endocytosis in plants by genetic means. We interfered with clathrin heavy chain
(CHC) function through mutants and dominant-negative approaches in Arabidopsis
thaliana and established tools to manipulate clathrin function in a cell type-specific
manner. The chc2 single mutants and dominant-negative CHC1 (HUB) transgenic lines
were defective in bulk endocytosis as well as in internalization of prominent
plasma membrane proteins. Interference with clathrin-mediated endocytosis led
to defects in constitutive endocytic recycling of PIN auxin transporters and their
polar distribution in embryos and roots. Consistent with this, these lines had
altered auxin distribution patterns and associated auxin transport-related phenotypes,
such as aberrant embryo patterning, imperfect cotyledon specification, agravitropic
growth, and impaired lateral root organogenesis. Together, these data demonstrate
a fundamental role for clathrin function in cell polarity, growth, patterning,
and organogenesis in plants.
author:
- first_name: Saeko
full_name: Kitakura, Saeko
last_name: Kitakura
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Christian
full_name: Löfke, Christian
last_name: Löfke
- first_name: Thomas
full_name: Teichmann, Thomas
last_name: Teichmann
- first_name: Hirokazu
full_name: Tanaka, Hirokazu
last_name: Tanaka
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kitakura S, Vanneste S, Robert S, et al. Clathrin mediates endocytosis and
polar distribution of PIN auxin transporters in Arabidopsis. Plant Cell.
2011;23(5):1920-1931. doi:10.1105/tpc.111.083030
apa: Kitakura, S., Vanneste, S., Robert, S., Löfke, C., Teichmann, T., Tanaka, H.,
& Friml, J. (2011). Clathrin mediates endocytosis and polar distribution of
PIN auxin transporters in Arabidopsis. Plant Cell. American Society of
Plant Biologists. https://doi.org/10.1105/tpc.111.083030
chicago: Kitakura, Saeko, Steffen Vanneste, Stéphanie Robert, Christian Löfke, Thomas
Teichmann, Hirokazu Tanaka, and Jiří Friml. “Clathrin Mediates Endocytosis and
Polar Distribution of PIN Auxin Transporters in Arabidopsis.” Plant Cell.
American Society of Plant Biologists, 2011. https://doi.org/10.1105/tpc.111.083030.
ieee: S. Kitakura et al., “Clathrin mediates endocytosis and polar distribution
of PIN auxin transporters in Arabidopsis,” Plant Cell, vol. 23, no. 5.
American Society of Plant Biologists, pp. 1920–1931, 2011.
ista: Kitakura S, Vanneste S, Robert S, Löfke C, Teichmann T, Tanaka H, Friml J.
2011. Clathrin mediates endocytosis and polar distribution of PIN auxin transporters
in Arabidopsis. Plant Cell. 23(5), 1920–1931.
mla: Kitakura, Saeko, et al. “Clathrin Mediates Endocytosis and Polar Distribution
of PIN Auxin Transporters in Arabidopsis.” Plant Cell, vol. 23, no. 5,
American Society of Plant Biologists, 2011, pp. 1920–31, doi:10.1105/tpc.111.083030.
short: S. Kitakura, S. Vanneste, S. Robert, C. Löfke, T. Teichmann, H. Tanaka, J.
Friml, Plant Cell 23 (2011) 1920–1931.
date_created: 2018-12-11T12:01:18Z
date_published: 2011-05-01T00:00:00Z
date_updated: 2021-01-12T07:40:57Z
day: '01'
doi: 10.1105/tpc.111.083030
extern: 1
intvolume: ' 23'
issue: '5'
month: '05'
page: 1920 - 1931
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3614'
quality_controlled: 0
status: public
title: Clathrin mediates endocytosis and polar distribution of PIN auxin transporters
in Arabidopsis
type: journal_article
volume: 23
year: '2011'
...
---
_id: '3088'
abstract:
- lang: eng
text: 'Background: Whereas the majority of animals develop toward a predetermined
body plan, plants show iterative growth and continually produce new organs and
structures from actively dividing meristems. This raises an intriguing question:
How are these newly developed organs patterned? In Arabidopsis embryos, radial
symmetry is broken by the bisymmetric specification of the cotyledons in the apical
domain. Subsequently, this bisymmetry is propagated to the root promeristem. Results:
Here we present a mutually inhibitory feedback loop between auxin and cytokinin
that sets distinct boundaries of hormonal output. Cytokinins promote the bisymmetric
distribution of the PIN-FORMED (PIN) auxin efflux proteins, which channel auxin
toward a central domain. High auxin promotes transcription of the cytokinin signaling
inhibitor AHP6, which closes the interaction loop. This bisymmetric auxin response
domain specifies the differentiation of protoxylem in a bisymmetric pattern. In
embryonic roots, cytokinin is required to translate a bisymmetric auxin response
in the cotyledons to a bisymmetric vascular pattern in the root promeristem. Conclusions:
Our results present an interactive feedback loop between hormonal signaling and
transport by which small biases in hormonal input are propagated into distinct
signaling domains to specify the vascular pattern in the root meristem. It is
an intriguing possibility that such a mechanism could transform radial patterns
and allow continuous vascular connections between other newly emerging organs.'
author:
- first_name: Anthony
full_name: Bishopp, Anthony
last_name: Bishopp
- first_name: Hanna
full_name: Help, Hanna
last_name: Help
- first_name: Sedeer
full_name: El-Showk, Sedeer
last_name: El Showk
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Ari
full_name: Mähönen, Ari Pekka
last_name: Mähönen
- first_name: Ykä
full_name: Helariutta, Ykä
last_name: Helariutta
citation:
ama: Bishopp A, Help H, El Showk S, et al. A mutually inhibitory interaction between
auxin and cytokinin specifies vascular pattern in roots. Current Biology.
2011;21(11):917-926. doi:10.1016/j.cub.2011.04.017
apa: Bishopp, A., Help, H., El Showk, S., Weijers, D., Scheres, B., Friml, J., …
Helariutta, Y. (2011). A mutually inhibitory interaction between auxin and cytokinin
specifies vascular pattern in roots. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2011.04.017
chicago: Bishopp, Anthony, Hanna Help, Sedeer El Showk, Dolf Weijers, Ben Scheres,
Jiří Friml, Eva Benková, Ari Mähönen, and Ykä Helariutta. “A Mutually Inhibitory
Interaction between Auxin and Cytokinin Specifies Vascular Pattern in Roots.”
Current Biology. Cell Press, 2011. https://doi.org/10.1016/j.cub.2011.04.017.
ieee: A. Bishopp et al., “A mutually inhibitory interaction between auxin
and cytokinin specifies vascular pattern in roots,” Current Biology, vol.
21, no. 11. Cell Press, pp. 917–926, 2011.
ista: Bishopp A, Help H, El Showk S, Weijers D, Scheres B, Friml J, Benková E, Mähönen
A, Helariutta Y. 2011. A mutually inhibitory interaction between auxin and cytokinin
specifies vascular pattern in roots. Current Biology. 21(11), 917–926.
mla: Bishopp, Anthony, et al. “A Mutually Inhibitory Interaction between Auxin and
Cytokinin Specifies Vascular Pattern in Roots.” Current Biology, vol. 21,
no. 11, Cell Press, 2011, pp. 917–26, doi:10.1016/j.cub.2011.04.017.
short: A. Bishopp, H. Help, S. El Showk, D. Weijers, B. Scheres, J. Friml, E. Benková,
A. Mähönen, Y. Helariutta, Current Biology 21 (2011) 917–926.
date_created: 2018-12-11T12:01:18Z
date_published: 2011-06-07T00:00:00Z
date_updated: 2021-01-12T07:40:58Z
day: '07'
doi: 10.1016/j.cub.2011.04.017
extern: 1
intvolume: ' 21'
issue: '11'
month: '06'
page: 917 - 926
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '3613'
quality_controlled: 0
status: public
title: A mutually inhibitory interaction between auxin and cytokinin specifies vascular
pattern in roots
type: journal_article
volume: 21
year: '2011'
...
---
_id: '3089'
abstract:
- lang: eng
text: The phytohormone auxin is an important determinant of plant development. Directional
auxin flow within tissues depends on polar localization of PIN auxin transporters.
To explore regulation of PIN-mediated auxin transport, we screened for suppressors
of PIN1 overexpression (supo) and identified an inositol polyphosphate 1-phosphatase
mutant (supo1), with elevated inositol trisphosphate (InsP 3) and cytosolic Ca
2+ levels. Pharmacological and genetic increases in InsP 3 or Ca 2+ levels also
suppressed the PIN1 gain-of-function phenotypes and caused defects in basal PIN
localization, auxin transport and auxin-mediated development. In contrast, the
reductions in InsP 3 levels and Ca 2+ signaling antagonized the effects of the
supo1 mutation and disrupted preferentially apical PIN localization. InsP 3 and
Ca 2+ are evolutionarily conserved second messengers involved in various cellular
functions, particularly stress responses. Our findings implicate them as modifiers
of cell polarity and polar auxin transport, and highlight a potential integration
point through which Ca 2+ signaling-related stimuli could influence auxin-mediated
development.
author:
- first_name: Jing
full_name: Zhang, Jing
last_name: Zhang
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Philip
full_name: Brewer, Philip B
last_name: Brewer
- first_name: Marta
full_name: Michniewicz, Marta
last_name: Michniewicz
- first_name: Peter
full_name: Peter Grones
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Christian
full_name: Löfke, Christian
last_name: Löfke
- first_name: Thomas
full_name: Teichmann, Thomas
last_name: Teichmann
- first_name: Agnieszka
full_name: Bielach, Agnieszka
last_name: Bielach
- first_name: Bernard
full_name: Cannoot, Bernard
last_name: Cannoot
- first_name: Klára
full_name: Hoyerová, Klára
last_name: Hoyerová
- first_name: Xu
full_name: Xu Chen
id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Hong
full_name: Xue, Hong-Wei
last_name: Xue
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang J, Vanneste S, Brewer P, et al. Inositol trisphosphate-induced ca^2+
signaling modulates auxin transport and pin polarity. Developmental Cell.
2011;20(6):855-866. doi:10.1016/j.devcel.2011.05.013
apa: Zhang, J., Vanneste, S., Brewer, P., Michniewicz, M., Grones, P., Kleine Vehn,
J., … Friml, J. (2011). Inositol trisphosphate-induced ca^2+ signaling modulates
auxin transport and pin polarity. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2011.05.013
chicago: Zhang, Jing, Steffen Vanneste, Philip Brewer, Marta Michniewicz, Peter
Grones, Jürgen Kleine Vehn, Christian Löfke, et al. “Inositol Trisphosphate-Induced
Ca^2+ Signaling Modulates Auxin Transport and Pin Polarity.” Developmental
Cell. Cell Press, 2011. https://doi.org/10.1016/j.devcel.2011.05.013.
ieee: J. Zhang et al., “Inositol trisphosphate-induced ca^2+ signaling modulates
auxin transport and pin polarity,” Developmental Cell, vol. 20, no. 6.
Cell Press, pp. 855–866, 2011.
ista: Zhang J, Vanneste S, Brewer P, Michniewicz M, Grones P, Kleine Vehn J, Löfke
C, Teichmann T, Bielach A, Cannoot B, Hoyerová K, Chen X, Xue H, Benková E, Zažímalová
E, Friml J. 2011. Inositol trisphosphate-induced ca^2+ signaling modulates auxin
transport and pin polarity. Developmental Cell. 20(6), 855–866.
mla: Zhang, Jing, et al. “Inositol Trisphosphate-Induced Ca^2+ Signaling Modulates
Auxin Transport and Pin Polarity.” Developmental Cell, vol. 20, no. 6,
Cell Press, 2011, pp. 855–66, doi:10.1016/j.devcel.2011.05.013.
short: J. Zhang, S. Vanneste, P. Brewer, M. Michniewicz, P. Grones, J. Kleine Vehn,
C. Löfke, T. Teichmann, A. Bielach, B. Cannoot, K. Hoyerová, X. Chen, H. Xue,
E. Benková, E. Zažímalová, J. Friml, Developmental Cell 20 (2011) 855–866.
date_created: 2018-12-11T12:01:18Z
date_published: 2011-06-14T00:00:00Z
date_updated: 2021-01-12T07:40:58Z
day: '14'
doi: 10.1016/j.devcel.2011.05.013
extern: 1
intvolume: ' 20'
issue: '6'
month: '06'
page: 855 - 866
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '3612'
quality_controlled: 0
status: public
title: Inositol trisphosphate-induced ca^2+ signaling modulates auxin transport and
pin polarity
type: journal_article
volume: 20
year: '2011'
...
---
_id: '3090'
abstract:
- lang: eng
text: The polarized transport of the phytohormone auxin [1], which is crucial for
the regulation of different stages of plant development [2, 3], depends on the
asymmetric plasma membrane distribution of the PIN-FORMED (PIN) auxin efflux carriers
[4, 5]. The PIN polar localization results from clathrin-mediated endocytosis
(CME) from the plasma membrane and subsequent polar recycling [6]. The Arabidopsis
genome encodes two groups of dynamin-related proteins (DRPs) that show homology
to mammalian dynamin - a protein required for fission of endocytic vesicles during
CME [7, 8]. Here we show by coimmunoprecipitation (coIP), bimolecular fluorescence
complementation (BiFC), and Förster resonance energy transfer (FRET) that members
of the DRP1 group closely associate with PIN proteins at the cell plate. Localization
and phenotypic analysis of novel drp1 mutants revealed a requirement for DRP1
function in correct PIN distribution and in auxin-mediated development. We propose
that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins
and the associated CME machinery from the cell plate membranes during cytokinesis
is an important mechanism for proper polar PIN positioning in interphase cells.
author:
- first_name: Jozef
full_name: Mravec, Jozef
last_name: Mravec
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Na
full_name: Li, Na
last_name: Li
- first_name: Sjef
full_name: Boeren, Sjef
last_name: Boeren
- first_name: Rumyana
full_name: Karlova, Rumyana
last_name: Karlova
- first_name: Saeko
full_name: Kitakura, Saeko
last_name: Kitakura
- first_name: Markéta
full_name: Pařezová, Markéta
last_name: Pařezová
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Thomasz
full_name: Nodzyński, Thomasz
last_name: Nodzyński
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Sebastian
full_name: Bednarek, Sebastian Y
last_name: Bednarek
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Sacco
full_name: De Vries, Sacco
last_name: De Vries
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mravec J, Petrášek J, Li N, et al. Cell plate restricted association of DRP1A
and PIN proteins is required for cell polarity establishment in arabidopsis. Current
Biology. 2011;21(12):1055-1060. doi:10.1016/j.cub.2011.05.018
apa: Mravec, J., Petrášek, J., Li, N., Boeren, S., Karlova, R., Kitakura, S., …
Friml, J. (2011). Cell plate restricted association of DRP1A and PIN proteins
is required for cell polarity establishment in arabidopsis. Current Biology.
Cell Press. https://doi.org/10.1016/j.cub.2011.05.018
chicago: Mravec, Jozef, Jan Petrášek, Na Li, Sjef Boeren, Rumyana Karlova, Saeko
Kitakura, Markéta Pařezová, et al. “Cell Plate Restricted Association of DRP1A
and PIN Proteins Is Required for Cell Polarity Establishment in Arabidopsis.”
Current Biology. Cell Press, 2011. https://doi.org/10.1016/j.cub.2011.05.018.
ieee: J. Mravec et al., “Cell plate restricted association of DRP1A and PIN
proteins is required for cell polarity establishment in arabidopsis,” Current
Biology, vol. 21, no. 12. Cell Press, pp. 1055–1060, 2011.
ista: Mravec J, Petrášek J, Li N, Boeren S, Karlova R, Kitakura S, Pařezová M, Naramoto
S, Nodzyński T, Dhonukshe P, Bednarek S, Zažímalová E, De Vries S, Friml J. 2011.
Cell plate restricted association of DRP1A and PIN proteins is required for cell
polarity establishment in arabidopsis. Current Biology. 21(12), 1055–1060.
mla: Mravec, Jozef, et al. “Cell Plate Restricted Association of DRP1A and PIN Proteins
Is Required for Cell Polarity Establishment in Arabidopsis.” Current Biology,
vol. 21, no. 12, Cell Press, 2011, pp. 1055–60, doi:10.1016/j.cub.2011.05.018.
short: J. Mravec, J. Petrášek, N. Li, S. Boeren, R. Karlova, S. Kitakura, M. Pařezová,
S. Naramoto, T. Nodzyński, P. Dhonukshe, S. Bednarek, E. Zažímalová, S. De Vries,
J. Friml, Current Biology 21 (2011) 1055–1060.
date_created: 2018-12-11T12:01:19Z
date_published: 2011-06-21T00:00:00Z
date_updated: 2021-01-12T07:40:59Z
day: '21'
doi: 10.1016/j.cub.2011.05.018
extern: 1
intvolume: ' 21'
issue: '12'
month: '06'
page: 1055 - 1060
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '3611'
quality_controlled: 0
status: public
title: Cell plate restricted association of DRP1A and PIN proteins is required for
cell polarity establishment in arabidopsis
type: journal_article
volume: 21
year: '2011'
...
---
_id: '3091'
author:
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Sauer M, Friml J. Fleeting hormone cues get stabilized for plant organogenesis.
Molecular Systems Biology. 2011;7. doi:10.1038/msb.2011.45
apa: Sauer, M., & Friml, J. (2011). Fleeting hormone cues get stabilized for
plant organogenesis. Molecular Systems Biology. Nature Publishing Group.
https://doi.org/10.1038/msb.2011.45
chicago: Sauer, Michael, and Jiří Friml. “Fleeting Hormone Cues Get Stabilized for
Plant Organogenesis.” Molecular Systems Biology. Nature Publishing Group,
2011. https://doi.org/10.1038/msb.2011.45.
ieee: M. Sauer and J. Friml, “Fleeting hormone cues get stabilized for plant organogenesis,”
Molecular Systems Biology, vol. 7. Nature Publishing Group, 2011.
ista: Sauer M, Friml J. 2011. Fleeting hormone cues get stabilized for plant organogenesis.
Molecular Systems Biology. 7.
mla: Sauer, Michael, and Jiří Friml. “Fleeting Hormone Cues Get Stabilized for Plant
Organogenesis.” Molecular Systems Biology, vol. 7, Nature Publishing Group,
2011, doi:10.1038/msb.2011.45.
short: M. Sauer, J. Friml, Molecular Systems Biology 7 (2011).
date_created: 2018-12-11T12:01:19Z
date_published: 2011-07-05T00:00:00Z
date_updated: 2021-01-12T07:41:00Z
day: '05'
doi: 10.1038/msb.2011.45
extern: '1'
external_id:
pmid:
- '21734646'
intvolume: ' 7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159970/
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
publication: Molecular Systems Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3610'
quality_controlled: '1'
status: public
title: Fleeting hormone cues get stabilized for plant organogenesis
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2011'
...
---
_id: '3092'
abstract:
- lang: eng
text: The phytohormone auxin is vital to plant growth and development. A unique
property of auxin among all other plant hormones is its cell-to-cell polar transport
that requires activity of polarly localized PIN-FORMED (PIN) auxin efflux transporters.
Despite the substantial molecular insight into the cellular PIN polarization,
the mechanistic understanding for developmentally and environmentally regulated
PIN polarization is scarce. The long-standing belief that auxin modulates its
own transport by means of a positive feedback mechanism has inspired both experimentalists
and theoreticians for more than two decades. Recently, theoretical models for
auxin-dependent patterning in plants include the feedback between auxin transport
and the PIN protein localization. These computer models aid to assess the complexity
of plant development by testing and predicting plausible scenarios for various
developmental processes that occur in planta. Although the majority of these models
rely on purely heuristic principles, the most recent mechanistic models tentatively
integrate biologically testable components into known cellular processes that
underlie the PIN polarity regulation. The existing and emerging computational
approaches to describe PIN polarization are presented and discussed in the light
of recent experimental data on the PIN polar targeting.
author:
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Willy
full_name: Govaerts, Willy
last_name: Govaerts
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
citation:
ama: 'Wabnik KT, Govaerts W, Friml J, Kleine Vehn J. Feedback models for polarized
auxin transport: An emerging trend. Molecular BioSystems. 2011;7(8):2352-2359.
doi:10.1039/c1mb05109a'
apa: 'Wabnik, K. T., Govaerts, W., Friml, J., & Kleine Vehn, J. (2011). Feedback
models for polarized auxin transport: An emerging trend. Molecular BioSystems.
Royal Society of Chemistry. https://doi.org/10.1039/c1mb05109a'
chicago: 'Wabnik, Krzysztof T, Willy Govaerts, Jiří Friml, and Jürgen Kleine Vehn.
“Feedback Models for Polarized Auxin Transport: An Emerging Trend.” Molecular
BioSystems. Royal Society of Chemistry, 2011. https://doi.org/10.1039/c1mb05109a.'
ieee: 'K. T. Wabnik, W. Govaerts, J. Friml, and J. Kleine Vehn, “Feedback models
for polarized auxin transport: An emerging trend,” Molecular BioSystems,
vol. 7, no. 8. Royal Society of Chemistry, pp. 2352–2359, 2011.'
ista: 'Wabnik KT, Govaerts W, Friml J, Kleine Vehn J. 2011. Feedback models for
polarized auxin transport: An emerging trend. Molecular BioSystems. 7(8), 2352–2359.'
mla: 'Wabnik, Krzysztof T., et al. “Feedback Models for Polarized Auxin Transport:
An Emerging Trend.” Molecular BioSystems, vol. 7, no. 8, Royal Society
of Chemistry, 2011, pp. 2352–59, doi:10.1039/c1mb05109a.'
short: K.T. Wabnik, W. Govaerts, J. Friml, J. Kleine Vehn, Molecular BioSystems
7 (2011) 2352–2359.
date_created: 2018-12-11T12:01:20Z
date_published: 2011-06-10T00:00:00Z
date_updated: 2021-01-12T07:41:00Z
day: '10'
doi: 10.1039/c1mb05109a
extern: '1'
external_id:
pmid:
- '21660355'
intvolume: ' 7'
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/21660355
month: '06'
oa: 1
oa_version: Published Version
page: 2352 - 2359
pmid: 1
publication: Molecular BioSystems
publication_status: published
publisher: Royal Society of Chemistry
publist_id: '3608'
quality_controlled: '1'
status: public
title: 'Feedback models for polarized auxin transport: An emerging trend'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2011'
...
---
_id: '3093'
abstract:
- lang: eng
text: |2-
Plants take up iron from the soil using the IRON-REGULATED TRANSPORTER 1 (IRT1) high-affinity iron transporter at the root surface. Sophisticated regulatory mechanisms allow plants to tightly control the levels of IRT1, ensuring optimal absorption of essential but toxic iron. Here, we demonstrate that overexpression of Arabidopsis thaliana IRT1 leads to constitutive IRT1 protein accumulation, metal overload, and oxidative stress. IRT1 is unexpectedly found in trans-Golgi network/early endosomes of root hair cells, and its levels and localization are unaffected by iron nutrition. Using pharmacological approaches, we show that IRT1 cycles to the plasma membrane to perform iron and metal uptake at the cell surface and is sent to the vacuole for proper turnover. We also prove that IRT1 is monoubiquitinated on several cytosol-exposed residues in vivo and that mutation of two putative monoubiquitination target residues in IRT1 triggers stabilization at the plasma membrane and leads to extreme lethality. Together, these data suggest a model in which monoubiquitin-dependent internalization/sorting and turnover keep the plasma membrane pool of IRT1 low to ensure proper iron uptake and to prevent metal toxicity. More generally, our work demonstrates the existence of monoubiquitin-dependent trafficking to lytic vacuoles in plants and points to proteasome-independent turnover of plasma membrane proteins.
author:
- first_name: Marie
full_name: Barberon, Marie
last_name: Barberon
- first_name: Enric
full_name: Zelazny, Enric
last_name: Zelazny
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Geneviève
full_name: Conéjéro, Geneviève
last_name: Conéjéro
- first_name: Cathy
full_name: Curie, Cathy
last_name: Curie
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Grégory
full_name: Vert, Grégory
last_name: Vert
citation:
ama: Barberon M, Zelazny E, Robert S, et al. Monoubiquitin dependent endocytosis
of the Iron Regulated Transporter 1 IRT1 transporter controls iron uptake in plants.
PNAS. 2011;108(32):E450-E458. doi:10.1073/pnas.1100659108
apa: Barberon, M., Zelazny, E., Robert, S., Conéjéro, G., Curie, C., Friml, J.,
& Vert, G. (2011). Monoubiquitin dependent endocytosis of the Iron Regulated
Transporter 1 IRT1 transporter controls iron uptake in plants. PNAS. National
Academy of Sciences. https://doi.org/10.1073/pnas.1100659108
chicago: Barberon, Marie, Enric Zelazny, Stéphanie Robert, Geneviève Conéjéro, Cathy
Curie, Jiří Friml, and Grégory Vert. “Monoubiquitin Dependent Endocytosis of the
Iron Regulated Transporter 1 IRT1 Transporter Controls Iron Uptake in Plants.”
PNAS. National Academy of Sciences, 2011. https://doi.org/10.1073/pnas.1100659108.
ieee: M. Barberon et al., “Monoubiquitin dependent endocytosis of the Iron
Regulated Transporter 1 IRT1 transporter controls iron uptake in plants,” PNAS,
vol. 108, no. 32. National Academy of Sciences, pp. E450–E458, 2011.
ista: Barberon M, Zelazny E, Robert S, Conéjéro G, Curie C, Friml J, Vert G. 2011.
Monoubiquitin dependent endocytosis of the Iron Regulated Transporter 1 IRT1 transporter
controls iron uptake in plants. PNAS. 108(32), E450–E458.
mla: Barberon, Marie, et al. “Monoubiquitin Dependent Endocytosis of the Iron Regulated
Transporter 1 IRT1 Transporter Controls Iron Uptake in Plants.” PNAS, vol.
108, no. 32, National Academy of Sciences, 2011, pp. E450–58, doi:10.1073/pnas.1100659108.
short: M. Barberon, E. Zelazny, S. Robert, G. Conéjéro, C. Curie, J. Friml, G. Vert,
PNAS 108 (2011) E450–E458.
date_created: 2018-12-11T12:01:20Z
date_published: 2011-08-09T00:00:00Z
date_updated: 2021-01-12T07:41:00Z
day: '09'
doi: 10.1073/pnas.1100659108
extern: 1
intvolume: ' 108'
issue: '32'
month: '08'
page: E450 - E458
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3607'
quality_controlled: 0
status: public
title: Monoubiquitin dependent endocytosis of the Iron Regulated Transporter 1 IRT1
transporter controls iron uptake in plants
type: journal_article
volume: 108
year: '2011'
...
---
_id: '3094'
abstract:
- lang: eng
text: Summary Gravitropism aligns plant growth with gravity. It involves gravity
perception and the asymmetric distribution of the phytohormone auxin. Here we
provide insights into the mechanism for hypocotyl gravitropic growth. We show
that the Arabidopsis thaliana PIN3 auxin transporter is required for the asymmetric
auxin distribution for the gravitropic response. Gravistimulation polarizes PIN3
to the bottom side of hypocotyl endodermal cells, which correlates with an increased
auxin response at the lower hypocotyl side. Both PIN3 polarization and hypocotyl
bending require the activity of the trafficking regulator GNOM and the protein
kinase PINOID. Our data suggest that gravity-induced PIN3 polarization diverts
the auxin flow to mediate the asymmetric distribution of auxin for gravitropic
shoot bending.
author:
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
- first_name: Javier
full_name: Gallego-Bartolomé, Javier
last_name: Gallego Bartolomé
- first_name: Marleen
full_name: Vanstraelen, Marleen
last_name: Vanstraelen
- first_name: Hélène
full_name: Robert, Hélène S
last_name: Robert
- first_name: David
full_name: Alabadí, David
last_name: Alabadí
- first_name: Miguel
full_name: Blázquez, Miguel A
last_name: Blázquez
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rakusová H, Gallego Bartolomé J, Vanstraelen M, et al. Polarization of PIN3
dependent auxin transport for hypocotyl gravitropic response in Arabidopsis thaliana.
Plant Journal. 2011;67(5):817-826. doi:10.1111/j.1365-313X.2011.04636.x
apa: Rakusová, H., Gallego Bartolomé, J., Vanstraelen, M., Robert, H., Alabadí,
D., Blázquez, M., … Friml, J. (2011). Polarization of PIN3 dependent auxin transport
for hypocotyl gravitropic response in Arabidopsis thaliana. Plant Journal.
Wiley-Blackwell. https://doi.org/10.1111/j.1365-313X.2011.04636.x
chicago: Rakusová, Hana, Javier Gallego Bartolomé, Marleen Vanstraelen, Hélène Robert,
David Alabadí, Miguel Blázquez, Eva Benková, and Jiří Friml. “Polarization of
PIN3 Dependent Auxin Transport for Hypocotyl Gravitropic Response in Arabidopsis
Thaliana.” Plant Journal. Wiley-Blackwell, 2011. https://doi.org/10.1111/j.1365-313X.2011.04636.x.
ieee: H. Rakusová et al., “Polarization of PIN3 dependent auxin transport
for hypocotyl gravitropic response in Arabidopsis thaliana,” Plant Journal,
vol. 67, no. 5. Wiley-Blackwell, pp. 817–826, 2011.
ista: Rakusová H, Gallego Bartolomé J, Vanstraelen M, Robert H, Alabadí D, Blázquez
M, Benková E, Friml J. 2011. Polarization of PIN3 dependent auxin transport for
hypocotyl gravitropic response in Arabidopsis thaliana. Plant Journal. 67(5),
817–826.
mla: Rakusová, Hana, et al. “Polarization of PIN3 Dependent Auxin Transport for
Hypocotyl Gravitropic Response in Arabidopsis Thaliana.” Plant Journal,
vol. 67, no. 5, Wiley-Blackwell, 2011, pp. 817–26, doi:10.1111/j.1365-313X.2011.04636.x.
short: H. Rakusová, J. Gallego Bartolomé, M. Vanstraelen, H. Robert, D. Alabadí,
M. Blázquez, E. Benková, J. Friml, Plant Journal 67 (2011) 817–826.
date_created: 2018-12-11T12:01:21Z
date_published: 2011-09-01T00:00:00Z
date_updated: 2021-01-12T07:41:01Z
day: '01'
doi: 10.1111/j.1365-313X.2011.04636.x
extern: 1
intvolume: ' 67'
issue: '5'
month: '09'
page: 817 - 826
publication: Plant Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3606'
quality_controlled: 0
status: public
title: Polarization of PIN3 dependent auxin transport for hypocotyl gravitropic response
in Arabidopsis thaliana
type: journal_article
volume: 67
year: '2011'
...
---
_id: '3095'
abstract:
- lang: eng
text: Root system architecture depends on lateral root (LR) initiation that takes
place in a relatively narrow developmental window (DW). Here, we analyzed the
role of auxin gradients established along the parent root in defining this DW
for LR initiation. Correlations between auxin distribution and response, and spatiotemporal
control of LR initiation were analyzed in Arabidopsis thaliana and tomato (Solanum
lycopersicum). In both Arabidopsis and tomato roots, a well defined zone, where
auxin content and response are minimal, demarcates the position of a DW for founder
cell specification and LR initiation. We show that in the zone of auxin minimum
pericycle cells have highest probability to become founder cells and that auxin
perception via the TIR1/AFB pathway, and polar auxin transport, are essential
for the establishment of this zone. Altogether, this study reveals that the same
morphogen-like molecule, auxin, can act simultaneously as a morphogenetic trigger
of LR founder cell identity and as a gradient-dependent signal defining positioning
of the founder cell specification. This auxin minimum zone might represent an
important control mechanism ensuring the LR initiation steadiness and the acropetal
LR initiation pattern. © 2011 The Authors. New Phytologist © 2011 New Phytologist
Trust.
author:
- first_name: Joseph
full_name: Dubrovsky, Joseph G
last_name: Dubrovsky
- first_name: Selene
full_name: Napsucialy-Mendivil, Selene
last_name: Napsucialy Mendivil
- first_name: Jérôme
full_name: Duclercq, Jérôme
last_name: Duclercq
- first_name: Yan
full_name: Cheng, Yan
last_name: Cheng
- first_name: Svetlana
full_name: Shishkova, Svetlana O
last_name: Shishkova
- first_name: Maria
full_name: Ivanchenko, Maria G
last_name: Ivanchenko
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Angus
full_name: Murphy, Angus S
last_name: Murphy
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Dubrovsky J, Napsucialy Mendivil S, Duclercq J, et al. Auxin minimum defines
a developmental window for lateral root initiation. New Phytologist. 2011;191(4):970-983.
doi: 10.1111/j.1469-8137.2011.03757.x
apa: Dubrovsky, J., Napsucialy Mendivil, S., Duclercq, J., Cheng, Y., Shishkova,
S., Ivanchenko, M., … Benková, E. (2011). Auxin minimum defines a developmental
window for lateral root initiation. New Phytologist. Wiley-Blackwell. https://doi.org/ 10.1111/j.1469-8137.2011.03757.x
chicago: Dubrovsky, Joseph, Selene Napsucialy Mendivil, Jérôme Duclercq, Yan Cheng,
Svetlana Shishkova, Maria Ivanchenko, Jiří Friml, Angus Murphy, and Eva Benková.
“Auxin Minimum Defines a Developmental Window for Lateral Root Initiation.” New
Phytologist. Wiley-Blackwell, 2011. https://doi.org/
10.1111/j.1469-8137.2011.03757.x.
ieee: J. Dubrovsky et al., “Auxin minimum defines a developmental window
for lateral root initiation,” New Phytologist, vol. 191, no. 4. Wiley-Blackwell,
pp. 970–983, 2011.
ista: Dubrovsky J, Napsucialy Mendivil S, Duclercq J, Cheng Y, Shishkova S, Ivanchenko
M, Friml J, Murphy A, Benková E. 2011. Auxin minimum defines a developmental window
for lateral root initiation. New Phytologist. 191(4), 970–983.
mla: Dubrovsky, Joseph, et al. “Auxin Minimum Defines a Developmental Window for
Lateral Root Initiation.” New Phytologist, vol. 191, no. 4, Wiley-Blackwell,
2011, pp. 970–83, doi:
10.1111/j.1469-8137.2011.03757.x.
short: J. Dubrovsky, S. Napsucialy Mendivil, J. Duclercq, Y. Cheng, S. Shishkova,
M. Ivanchenko, J. Friml, A. Murphy, E. Benková, New Phytologist 191 (2011) 970–983.
date_created: 2018-12-11T12:01:21Z
date_published: 2011-01-01T00:00:00Z
date_updated: 2021-01-12T07:41:01Z
day: '01'
doi: ' 10.1111/j.1469-8137.2011.03757.x'
extern: 1
intvolume: ' 191'
issue: '4'
month: '01'
page: 970 - 983
publication: New Phytologist
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3605'
quality_controlled: 0
status: public
title: Auxin minimum defines a developmental window for lateral root initiation
type: journal_article
volume: 191
year: '2011'
...
---
_id: '3096'
abstract:
- lang: eng
text: Carrier-dependent, intercellular auxin transport is central to the developmental
patterning of higher plants (tracheophytes). The evolution of this polar auxin
transport might be linked to the translocation of some PIN auxin efflux carriers
from their presumably ancestral localization at the endoplasmic reticulum (ER)
to the polar domains at the plasma membrane. Here we propose an eventually ancient
mechanism of intercellular auxin distribution by ER-localized auxin transporters
involving intracellular auxin retention and switch-like release from the ER. The
proposed model integrates feedback circuits utilizing the conserved nuclear auxin
signaling for the regulation of PIN transcription and a hypothetical ER-based
signaling for the regulation of PIN-dependent transport activity at the ER. Computer
simulations of the model revealed its plausibility for generating auxin channels
and localized auxin maxima highlighting the possibility of this alternative mechanism
for polar auxin transport.
author:
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Willy
full_name: Govaerts, Willy
last_name: Govaerts
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Wabnik KT, Kleine Vehn J, Govaerts W, Friml J. Prototype cell-to-cell auxin
transport mechanism by intracellular auxin compartmentalization. Trends in
Plant Science. 2011;16(9):468-475. doi:10.1016/j.tplants.2011.05.002
apa: Wabnik, K. T., Kleine Vehn, J., Govaerts, W., & Friml, J. (2011). Prototype
cell-to-cell auxin transport mechanism by intracellular auxin compartmentalization.
Trends in Plant Science. Cell Press. https://doi.org/10.1016/j.tplants.2011.05.002
chicago: Wabnik, Krzysztof T, Jürgen Kleine Vehn, Willy Govaerts, and Jiří Friml.
“Prototype Cell-to-Cell Auxin Transport Mechanism by Intracellular Auxin Compartmentalization.”
Trends in Plant Science. Cell Press, 2011. https://doi.org/10.1016/j.tplants.2011.05.002.
ieee: K. T. Wabnik, J. Kleine Vehn, W. Govaerts, and J. Friml, “Prototype cell-to-cell
auxin transport mechanism by intracellular auxin compartmentalization,” Trends
in Plant Science, vol. 16, no. 9. Cell Press, pp. 468–475, 2011.
ista: Wabnik KT, Kleine Vehn J, Govaerts W, Friml J. 2011. Prototype cell-to-cell
auxin transport mechanism by intracellular auxin compartmentalization. Trends
in Plant Science. 16(9), 468–475.
mla: Wabnik, Krzysztof T., et al. “Prototype Cell-to-Cell Auxin Transport Mechanism
by Intracellular Auxin Compartmentalization.” Trends in Plant Science,
vol. 16, no. 9, Cell Press, 2011, pp. 468–75, doi:10.1016/j.tplants.2011.05.002.
short: K.T. Wabnik, J. Kleine Vehn, W. Govaerts, J. Friml, Trends in Plant Science
16 (2011) 468–475.
date_created: 2018-12-11T12:01:21Z
date_published: 2011-09-01T00:00:00Z
date_updated: 2021-01-12T07:41:01Z
day: '01'
doi: 10.1016/j.tplants.2011.05.002
extern: '1'
intvolume: ' 16'
issue: '9'
language:
- iso: eng
month: '09'
oa_version: None
page: 468 - 475
publication: Trends in Plant Science
publication_status: published
publisher: Cell Press
publist_id: '3604'
quality_controlled: '1'
status: public
title: Prototype cell-to-cell auxin transport mechanism by intracellular auxin compartmentalization
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2011'
...
---
_id: '3097'
abstract:
- lang: eng
text: Cytokinin is an important regulator of plant growth and development. In Arabidopsis
thaliana, the two-component phosphorelay mediated through a family of histidine
kinases and response regulators is recognized as the principal cytokinin signal
transduction mechanism activating the complex transcriptional response to control
various developmental processes. Here, we identified an alternative mode of cytokinin
action that uses endocytic trafficking as a means to direct plant organogenesis.
This activity occurs downstream of known cytokinin receptors but through a branch
of the cytokinin signaling pathway that does not involve transcriptional regulation.
We show that cytokinin regulates endocytic recycling of the auxin efflux carrier
PINFORMED1 (PIN1) by redirecting it for lytic degradation in vacuoles. Stimulation
of the lytic PIN1 degradation is not a default effect for general downregulation
of proteins from plasma membranes, but a specific mechanism to rapidly modulate
the auxin distribution in cytokinin-mediated developmental processes.
author:
- first_name: Peter
full_name: Peter Marhavy
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Agnieszka
full_name: Bielach, Agnieszka
last_name: Bielach
- first_name: Lindy
full_name: Abas, Lindy
last_name: Abas
- first_name: Anas
full_name: Abuzeineh, Anas
last_name: Abuzeineh
- first_name: Jérôme
full_name: Duclercq, Jérôme
last_name: Duclercq
- first_name: Hirokazu
full_name: Tanaka, Hirokazu
last_name: Tanaka
- first_name: Markéta
full_name: Pařezová, Markéta
last_name: Pařezová
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Marhavý P, Bielach A, Abas L, et al. Cytokinin modulates endocytic trafficking
of PIN1 auxin efflux carrier to control plant organogenesis. Developmental
Cell. 2011;21(4):796-804. doi:10.1016/j.devcel.2011.08.014
apa: Marhavý, P., Bielach, A., Abas, L., Abuzeineh, A., Duclercq, J., Tanaka, H.,
… Benková, E. (2011). Cytokinin modulates endocytic trafficking of PIN1 auxin
efflux carrier to control plant organogenesis. Developmental Cell. Cell
Press. https://doi.org/10.1016/j.devcel.2011.08.014
chicago: Marhavý, Peter, Agnieszka Bielach, Lindy Abas, Anas Abuzeineh, Jérôme Duclercq,
Hirokazu Tanaka, Markéta Pařezová, et al. “Cytokinin Modulates Endocytic Trafficking
of PIN1 Auxin Efflux Carrier to Control Plant Organogenesis.” Developmental
Cell. Cell Press, 2011. https://doi.org/10.1016/j.devcel.2011.08.014.
ieee: P. Marhavý et al., “Cytokinin modulates endocytic trafficking of PIN1
auxin efflux carrier to control plant organogenesis,” Developmental Cell,
vol. 21, no. 4. Cell Press, pp. 796–804, 2011.
ista: Marhavý P, Bielach A, Abas L, Abuzeineh A, Duclercq J, Tanaka H, Pařezová
M, Petrášek J, Friml J, Kleine Vehn J, Benková E. 2011. Cytokinin modulates endocytic
trafficking of PIN1 auxin efflux carrier to control plant organogenesis. Developmental
Cell. 21(4), 796–804.
mla: Marhavý, Peter, et al. “Cytokinin Modulates Endocytic Trafficking of PIN1 Auxin
Efflux Carrier to Control Plant Organogenesis.” Developmental Cell, vol.
21, no. 4, Cell Press, 2011, pp. 796–804, doi:10.1016/j.devcel.2011.08.014.
short: P. Marhavý, A. Bielach, L. Abas, A. Abuzeineh, J. Duclercq, H. Tanaka, M.
Pařezová, J. Petrášek, J. Friml, J. Kleine Vehn, E. Benková, Developmental Cell
21 (2011) 796–804.
date_created: 2018-12-11T12:01:22Z
date_published: 2011-10-18T00:00:00Z
date_updated: 2021-01-12T07:41:02Z
day: '18'
doi: 10.1016/j.devcel.2011.08.014
extern: 1
intvolume: ' 21'
issue: '4'
month: '10'
page: 796 - 804
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '3603'
quality_controlled: 0
status: public
title: Cytokinin modulates endocytic trafficking of PIN1 auxin efflux carrier to control
plant organogenesis
type: journal_article
volume: 21
year: '2011'
...
---
_id: '3098'
abstract:
- lang: eng
text: Cell polarity reflected by asymmetric distribution of proteins at the plasma
membrane is a fundamental feature of unicellular and multicellular organisms.
It remains conceptually unclear how cell polarity is kept in cell wall-encapsulated
plant cells. We have used super-resolution and semi-quantitative live-cell imaging
in combination with pharmacological, genetic, and computational approaches to
reveal insights into the mechanism of cell polarity maintenance in Arabidopsis
thaliana. We show that polar-competent PIN transporters for the phytohormone auxin
are delivered to the center of polar domains by super-polar recycling. Within
the plasma membrane, PINs are recruited into non-mobile membrane clusters and
their lateral diffusion is dramatically reduced, which ensures longer polar retention.
At the circumventing edges of the polar domain, spatially defined internalization
of escaped cargos occurs by clathrin-dependent endocytosis. Computer simulations
confirm that the combination of these processes provides a robust mechanism for
polarity maintenance in plant cells. Moreover, our study suggests that the regulation
of lateral diffusion and spatially defined endocytosis, but not super-polar exocytosis
have primary importance for PIN polarity maintenance.
author:
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Krzysztof T
full_name: Krzysztof Wabnik
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Alexandre
full_name: Martinière, Alexandre
last_name: Martinière
- first_name: Łukasz
full_name: Łangowski, Łukasz
last_name: Łangowski
- first_name: Katrin
full_name: Willig, Katrin
last_name: Willig
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Johannes
full_name: Leitner, Johannes
last_name: Leitner
- first_name: Hirokazu
full_name: Tanaka, Hirokazu
last_name: Tanaka
- first_name: Stefan
full_name: Jakobs, Stefan
last_name: Jakobs
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Willy
full_name: Govaerts, Willy J
last_name: Govaerts
- first_name: Stefan
full_name: Hell, Stefan W
last_name: Hell
- first_name: John
full_name: Runions, John
last_name: Runions
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kleine Vehn J, Wabnik KT, Martinière A, et al. Recycling, clustering and endocytosis
jointly maintain PIN auxin carrier polarity at the plasma membrane. Molecular
Systems Biology. 2011;7. doi:10.1038/msb.2011.72
apa: Kleine Vehn, J., Wabnik, K. T., Martinière, A., Łangowski, Ł., Willig, K.,
Naramoto, S., … Friml, J. (2011). Recycling, clustering and endocytosis jointly
maintain PIN auxin carrier polarity at the plasma membrane. Molecular Systems
Biology. Nature Publishing Group. https://doi.org/10.1038/msb.2011.72
chicago: Kleine Vehn, Jürgen, Krzysztof T Wabnik, Alexandre Martinière, Łukasz Łangowski,
Katrin Willig, Satoshi Naramoto, Johannes Leitner, et al. “Recycling, Clustering
and Endocytosis Jointly Maintain PIN Auxin Carrier Polarity at the Plasma Membrane.”
Molecular Systems Biology. Nature Publishing Group, 2011. https://doi.org/10.1038/msb.2011.72.
ieee: J. Kleine Vehn et al., “Recycling, clustering and endocytosis jointly
maintain PIN auxin carrier polarity at the plasma membrane,” Molecular Systems
Biology, vol. 7. Nature Publishing Group, 2011.
ista: Kleine Vehn J, Wabnik KT, Martinière A, Łangowski Ł, Willig K, Naramoto S,
Leitner J, Tanaka H, Jakobs S, Robert S, Luschnig C, Govaerts W, Hell S, Runions
J, Friml J. 2011. Recycling, clustering and endocytosis jointly maintain PIN auxin
carrier polarity at the plasma membrane. Molecular Systems Biology. 7.
mla: Kleine Vehn, Jürgen, et al. “Recycling, Clustering and Endocytosis Jointly
Maintain PIN Auxin Carrier Polarity at the Plasma Membrane.” Molecular Systems
Biology, vol. 7, Nature Publishing Group, 2011, doi:10.1038/msb.2011.72.
short: J. Kleine Vehn, K.T. Wabnik, A. Martinière, Ł. Łangowski, K. Willig, S. Naramoto,
J. Leitner, H. Tanaka, S. Jakobs, S. Robert, C. Luschnig, W. Govaerts, S. Hell,
J. Runions, J. Friml, Molecular Systems Biology 7 (2011).
date_created: 2018-12-11T12:01:22Z
date_published: 2011-10-25T00:00:00Z
date_updated: 2021-01-12T07:41:02Z
day: '25'
doi: 10.1038/msb.2011.72
extern: 1
intvolume: ' 7'
month: '10'
publication: Molecular Systems Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3601'
quality_controlled: 0
status: public
title: Recycling, clustering and endocytosis jointly maintain PIN auxin carrier polarity
at the plasma membrane
type: journal_article
volume: 7
year: '2011'
...
---
_id: '3099'
abstract:
- lang: eng
text: Endomembrane trafficking relies on the coordination of a highly complex, dynamic
network of intracellular vesicles. Understanding the network will require a dissection
of cargo and vesicle dynamics at the cellular level in vivo. This is also a key
to establishing a link between vesicular networks and their functional roles in
development. We used a high-content intracellular screen to discover small molecules
targeting endomembrane trafficking in vivo in a complex eukaryote, Arabidopsis
thaliana. Tens of thousands of molecules were prescreened and a selected subset
was interrogated against a panel of plasma membrane (PM) and other endomembrane
compartment markers to identify molecules that altered vesicle trafficking. The
extensive image dataset was transformed by a flexible algorithm into a marker-by-phenotype-by-treatment
time matrix and revealed groups of molecules that induced similar subcellular
fingerprints (clusters). This matrix provides a platform for a systems view of
trafficking. Molecules from distinct clusters presented avenues and enabled an
entry point to dissect recycling at the PM, vacuolar sorting, and cell-plate maturation.
Bioactivity in human cells indicated the value of the approach to identifying
small molecules that are active in diverse organisms for biology and drug discovery.
author:
- first_name: Georgia
full_name: Drakakaki, Georgia
last_name: Drakakaki
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Anna
full_name: Szatmári, Anna-Maria
last_name: Szatmári
- first_name: Michelle
full_name: Brown, Michelle Q
last_name: Brown
- first_name: Shingo
full_name: Nagawa, Shingo
last_name: Nagawa
- first_name: Daniël
full_name: Van Damme, Daniël
last_name: Van Damme
- first_name: Marylin
full_name: Leonard, Marylin
last_name: Leonard
- first_name: Zhenbiao
full_name: Yang, Zhenbiao
last_name: Yang
- first_name: Thomas
full_name: Girke, Thomas
last_name: Girke
- first_name: Sandra
full_name: Schmid, Sandra L
last_name: Schmid
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Natasha
full_name: Raikhel, Natasha V
last_name: Raikhel
- first_name: Glen
full_name: Hicks, Glen R
last_name: Hicks
citation:
ama: Drakakaki G, Robert S, Szatmári A, et al. Clusters of bioactive compounds target
dynamic endomembrane networks in vivo. PNAS. 2011;108(43):17850-17855.
doi:10.1073/pnas.1108581108
apa: Drakakaki, G., Robert, S., Szatmári, A., Brown, M., Nagawa, S., Van Damme,
D., … Hicks, G. (2011). Clusters of bioactive compounds target dynamic endomembrane
networks in vivo. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1108581108
chicago: Drakakaki, Georgia, Stéphanie Robert, Anna Szatmári, Michelle Brown, Shingo
Nagawa, Daniël Van Damme, Marylin Leonard, et al. “Clusters of Bioactive Compounds
Target Dynamic Endomembrane Networks in Vivo.” PNAS. National Academy of
Sciences, 2011. https://doi.org/10.1073/pnas.1108581108.
ieee: G. Drakakaki et al., “Clusters of bioactive compounds target dynamic
endomembrane networks in vivo,” PNAS, vol. 108, no. 43. National Academy
of Sciences, pp. 17850–17855, 2011.
ista: Drakakaki G, Robert S, Szatmári A, Brown M, Nagawa S, Van Damme D, Leonard
M, Yang Z, Girke T, Schmid S, Russinova E, Friml J, Raikhel N, Hicks G. 2011.
Clusters of bioactive compounds target dynamic endomembrane networks in vivo.
PNAS. 108(43), 17850–17855.
mla: Drakakaki, Georgia, et al. “Clusters of Bioactive Compounds Target Dynamic
Endomembrane Networks in Vivo.” PNAS, vol. 108, no. 43, National Academy
of Sciences, 2011, pp. 17850–55, doi:10.1073/pnas.1108581108.
short: G. Drakakaki, S. Robert, A. Szatmári, M. Brown, S. Nagawa, D. Van Damme,
M. Leonard, Z. Yang, T. Girke, S. Schmid, E. Russinova, J. Friml, N. Raikhel,
G. Hicks, PNAS 108 (2011) 17850–17855.
date_created: 2018-12-11T12:01:23Z
date_published: 2011-10-25T00:00:00Z
date_updated: 2021-01-12T07:41:02Z
day: '25'
doi: 10.1073/pnas.1108581108
extern: 1
intvolume: ' 108'
issue: '43'
month: '10'
page: 17850 - 17855
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3602'
quality_controlled: 0
status: public
title: Clusters of bioactive compounds target dynamic endomembrane networks in vivo
type: journal_article
volume: 108
year: '2011'
...