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Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20964\">10.15479/AT-ISTA-20964</a>","ieee":"D. Vladimirtsev, “Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels,” Institute of Science and Technology Austria, 2026.","chicago":"Vladimirtsev, Dmitrii. “Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels.” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-20964\">https://doi.org/10.15479/AT-ISTA-20964</a>.","mla":"Vladimirtsev, Dmitrii. <i>Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels</i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20964\">10.15479/AT-ISTA-20964</a>.","apa":"Vladimirtsev, D. (2026). <i>Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20964\">https://doi.org/10.15479/AT-ISTA-20964</a>","ista":"Vladimirtsev D. 2026. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. Institute of Science and Technology Austria.","short":"D. Vladimirtsev, Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels, Institute of Science and Technology Austria, 2026."},"day":"14","page":"22","month":"01"},{"external_id":{"oaworkid":["W7118187193"]},"ec_funded":1,"OA_type":"hybrid","date_published":"2026-01-05T00:00:00Z","date_created":"2026-01-20T10:12:19Z","file":[{"creator":"dernst","relation":"main_file","file_name":"2026_NaturePhysics_Mishra.pdf","file_size":7335694,"success":1,"date_created":"2026-01-21T08:21:11Z","file_id":"21026","date_updated":"2026-01-21T08:21:11Z","access_level":"open_access","content_type":"application/pdf","checksum":"0ab7ac2fbcb61a364dba57152db64ed7"}],"publication":"Nature Physics","publication_identifier":{"issnl":[" 1745-2473"],"eissn":["1745-2481"],"issn":["1745-2473"]},"author":[{"id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E","full_name":"Mishra, Nikhil","last_name":"Mishra","first_name":"Nikhil","orcid":"0000-0002-6425-5788"},{"id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","full_name":"Li, Yuting I","first_name":"Yuting I","last_name":"Li"},{"orcid":"0000-0001-6005-1561","first_name":"Edouard B","full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"year":"2026","date_updated":"2026-04-28T12:55:30Z","language":[{"iso":"eng"}],"status":"public","oa_version":"Published Version","doi":"10.1038/s41567-025-03122-1","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"quality_controlled":"1","oaworkid":1,"has_accepted_license":"1","related_material":{"link":[{"relation":"research_data","description":"News on ISTA website","url":"https://ista.ac.at/en/news/geometry-shapes-life/"}]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"corr_author":"1","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","title":"Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo","_id":"21015","volume":22,"type":"journal_article","acknowledgement":"We thank N. Petridou (EMBL) for sharing results before publication. N.M. was supported by funding from the European Union’s Horizon 2020 programme under the Marie Skłodowska-Curie COFUND Actions ISTplus grant agreement number 754411. Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement number 101034413. The research was supported by funding to C.-P.H. from the NOMIS Foundation, Project ID 1.844. We would like to thank past and present members of the Heisenberg and Hannezo groups for discussions, particularly S. Shamipour, V. Doddihal, M. Jovic, N. Hino, F. N. Arslan, R. Kobylinska and C. Camelo for feedback on the draft manuscript. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria through resources provided by the Aquatics Facility, Imaging & Optics Facility (IOF), Scientific Computing (SciComp) facility and Lab Support Facility (LSF). Open access funding provided by Institute of Science and Technology (IST Austria).","project":[{"call_identifier":"H2020","_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships"},{"call_identifier":"H2020","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413","name":"IST-BRIDGE: International postdoctoral program"},{"name":"Cytoplasmic self-organization into cell-like compartments as a common guiding principle in early animal development","_id":"917c023a-16d5-11f0-9cad-eb5cafc52090"}],"PlanS_conform":"1","publisher":"Springer Nature","intvolume":"        22","file_date_updated":"2026-01-21T08:21:11Z","publication_status":"published","OA_place":"publisher","department":[{"_id":"EdHa"},{"_id":"CaHe"}],"article_processing_charge":"Yes (via OA deal)","scopus_import":"1","abstract":[{"lang":"eng","text":"Early embryo geometry is one of the most invariant species-specific traits, yet its role in ensuring developmental reproducibility and robustness remains underexplored. Here we show that in zebrafish, the geometry of the fertilized egg—specifically its curvature and volume—serves as a critical initial condition triggering a cascade of events that influence development. The embryo geometry guides patterned asymmetric cell divisions in the blastoderm, generating radial gradients of cell volume and nucleocytoplasmic ratio. These gradients generate mitotic phase waves, with the nucleocytoplasmic ratio determining individual cell cycle periods independently of other cells. We demonstrate that reducing cell autonomy reshapes these waves, emphasizing the instructive role of geometry-derived volume patterns in setting the intrinsic period of the cell cycle oscillator. In addition to organizing cell cycles, early embryo geometry spatially patterns zygotic genome activation at the midblastula transition, a key step in establishing embryonic autonomy. Disrupting the embryo shape alters the zygotic genome activation pattern and causes ectopic germ layer specification, underscoring the developmental significance of geometry. Together, our findings reveal a symmetry-breaking function of early embryo geometry in coordinating cell cycle and transcriptional patterning."}],"citation":{"chicago":"Mishra, Nikhil, Yuting I Li, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>.","ieee":"N. Mishra, Y. I. Li, E. B. Hannezo, and C.-P. J. Heisenberg, “Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo,” <i>Nature Physics</i>, vol. 22. Springer Nature, pp. 139–150, 2026.","ama":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. 2026;22:139-150. doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>","mla":"Mishra, Nikhil, et al. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>, vol. 22, Springer Nature, 2026, pp. 139–50, doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>.","short":"N. Mishra, Y.I. Li, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 22 (2026) 139–150.","ista":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. 2026. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. Nature Physics. 22, 139–150.","apa":"Mishra, N., Li, Y. I., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2026). Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>"},"article_type":"original","ddc":["570"],"page":"139-150","day":"05","month":"01","oa":1},{"has_accepted_license":"1","tmp":{"short":"CC BY-SA (4.0)","image":"/images/cc_by_sa.png","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode"},"corr_author":"1","user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","title":"Data associated with Keratins coordinate tissue spreading ","_id":"21137","type":"research_data","acknowledgement":"We thank all members of the Heisenberg, Henkes, and Hannezo groups for their support. We are also grateful to the Imaging and Optics, Scientific Computing, Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their technical assistance and support. Numerical simulations were performed using the computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020 research and innovation programme (grant agreement No. 665385). This work was supported by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to C.-P.H.","project":[{"call_identifier":"H2020","name":"International IST Doctoral Program","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"name":"Keratins in epithelial tissue spreading","grant_number":"PAT 5044023","_id":"8f060199-16d5-11f0-9cad-f3253b266c46"},{"call_identifier":"FWF","name":"Nano-Analytics of Cellular Systems","_id":"252C3B08-B435-11E9-9278-68D0E5697425","grant_number":"W1250-B20"}],"license":"https://creativecommons.org/licenses/by-sa/4.0/","ec_funded":1,"date_published":"2026-03-24T00:00:00Z","file":[{"access_level":"open_access","content_type":"application/zip","date_updated":"2026-03-16T11:51:10Z","file_id":"21461","checksum":"5d1fda7e410f24c311fcf6bcf725698f","file_size":725916,"description":"Python3 library written in C++20 to integrate vertex models. Please read the readme at https://github.com/yketa/cells/blob/main/README.md for detailed instructions for installation and usage of the code in this repository. 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Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>.","ama":"Naik S. Data associated with Keratins coordinate tissue spreading . 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>","chicago":"Naik, Suyash. “Data Associated with Keratins Coordinate Tissue Spreading .” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">https://doi.org/10.15479/AT-ISTA-21137</a>.","ieee":"S. Naik, “Data associated with Keratins coordinate tissue spreading .” Institute of Science and Technology Austria, 2026.","apa":"Naik, S. (2026). Data associated with Keratins coordinate tissue spreading . Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">https://doi.org/10.15479/AT-ISTA-21137</a>","ista":"Naik S. 2026. Data associated with Keratins coordinate tissue spreading , Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>.","short":"S. Naik, (2026)."},"day":"24","month":"3","oa":1,"contributor":[{"contributor_type":"researcher","first_name":"Yann-Edwin","last_name":"Keta"},{"contributor_type":"supervisor","last_name":"Henkes","first_name":"Silke "},{"contributor_type":"supervisor","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"},{"last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","contributor_type":"supervisor","orcid":"0000-0001-6005-1561"}],"publisher":"Institute of Science and Technology Austria","file_date_updated":"2026-03-24T07:21:43Z","OA_place":"repository","department":[{"_id":"GradSch"},{"_id":"CaHe"},{"_id":"EdHa"}],"article_processing_charge":"No"},{"article_processing_charge":"No","OA_place":"repository","publication_status":"submitted","department":[{"_id":"SiHi"},{"_id":"LoSw"}],"oa":1,"month":"02","main_file_link":[{"url":"https://doi.org/10.64898/2026.02.12.705305","open_access":"1"}],"day":"16","ddc":["570"],"citation":{"ista":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, Smits MG, Streicher C, Cheung GT, Hippenmeyer S, Sweeney LB. Lineage origin of spinal cord cell type diversity. bioRxiv, <a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>.","apa":"Gobeil, S. A., Da Silveira Neto, F., Silvestrelli, G., Smits, M. G., Streicher, C., Cheung, G. T., … Sweeney, L. B. (n.d.). Lineage origin of spinal cord cell type diversity. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.02.12.705305\">https://doi.org/10.64898/2026.02.12.705305</a>","short":"S.A. Gobeil, F. Da Silveira Neto, G. Silvestrelli, M.G. Smits, C. Streicher, G.T. Cheung, S. Hippenmeyer, L.B. Sweeney, BioRxiv (n.d.).","ieee":"S. A. Gobeil <i>et al.</i>, “Lineage origin of spinal cord cell type diversity,” <i>bioRxiv</i>. .","ama":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, et al. Lineage origin of spinal cord cell type diversity. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>","chicago":"Gobeil, Sophie A, Francisco Da Silveira Neto, Giulia Silvestrelli, Matthijs Geert Smits, Carmen Streicher, Giselle T Cheung, Simon Hippenmeyer, and Lora B. Sweeney. “Lineage Origin of Spinal Cord Cell Type Diversity.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.02.12.705305\">https://doi.org/10.64898/2026.02.12.705305</a>.","mla":"Gobeil, Sophie A., et al. “Lineage Origin of Spinal Cord Cell Type Diversity.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>."},"abstract":[{"text":"The complexity and specificity of movement in vertebrates is driven by a rich diversity of spinal motor and interneuron cell types. During development, eleven spinal cord progenitor domains generate an equivalent number of cardinal neuron types. How progenitor domains, individual progenitors, and post-mitotic diversity relate is still unknown. We performed high-resolution, single-progenitor cell lineage tracing in the embryonic mouse spinal cord using mosaic analysis with double markers (MADM). Our quantitative study of lineage progression revealed that spinal cord progenitors undergo highly variable numbers of proliferative, neurogenic, and gliogenic cell divisions. The nascent clonally-related neurons migrate radially over large distances, span the dorsoventral axis, and even cross the midline, demonstrating striking bilaterality. Molecular and morphometric analysis indicate high levels of progenitor multipotency, with an individual progenitor capable of producing several molecularly and morphologically distinct neuron types, as well as astrocytes. These findings redefine spinal cord development as a process in which lineage variability — rather than rigid progenitor identity — drives the generation of cellular diversity.","lang":"eng"}],"oa_version":"Preprint","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"doi":"10.64898/2026.02.12.705305","status":"public","language":[{"iso":"eng"}],"date_updated":"2026-04-14T08:16:55Z","year":"2026","author":[{"first_name":"Sophie A","full_name":"Gobeil, Sophie A","id":"2f3e9efb-eb24-11ec-86b2-88efb11d59fa","last_name":"Gobeil"},{"full_name":"Da Silveira Neto, Francisco","id":"8cfb7412-10a7-11f1-add1-82b44e6418f2","first_name":"Francisco","last_name":"Da Silveira Neto"},{"first_name":"Giulia","id":"12632ae8-799e-11ef-94a2-e5a3b5ef49e9","last_name":"Silvestrelli","full_name":"Silvestrelli, Giulia"},{"first_name":"Matthijs Geert","id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","last_name":"Smits","full_name":"Smits, Matthijs Geert"},{"full_name":"Streicher, Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher","first_name":"Carmen"},{"orcid":"0000-0001-8457-2572","first_name":"Giselle T","last_name":"Cheung","full_name":"Cheung, Giselle T","id":"471195F6-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-2279-1061","full_name":"Hippenmeyer, Simon","last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","first_name":"Simon"},{"orcid":"0000-0001-9242-5601","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney"}],"publication":"bioRxiv","date_created":"2026-02-17T11:36:20Z","date_published":"2026-02-16T00:00:00Z","OA_type":"green","project":[{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551","name":"Development and Evolution of Tetrapod Motor Circuits"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","grant_number":"F7814","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e"},{"grant_number":"F7805","_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression"}],"acknowledgement":"We would like to thank Elizabeth Marin, Anna Kicheva, Igor Adameyko, and James Briscoe as\r\nwell as members of the Sweeney and Hippemeyer labs and SFB consortium for comments on\r\nthe manuscript. We are also grateful for the technical support of the Preclinical and Imaging and\r\nOptics Facilities support teams (ISTA). In addition, we thank our funding sources for providing\r\nthe resources to do these experiments: Horizon Europe ERC Starting Grant Number 101041551\r\n(M.S.; L.B.S.); Special Research Program (SFB) of the Austrian Science Fund (FWF)\r\nNeuroStem Modulation Project numbers F7814-B (S.A.G.; M.S.; G.S.; and L.B.S.) and F7805\r\n(G.C. and S.H.). S.A.G is supported by a Boehringer Ingelheim Fonds PhD Fellowship, F.D.S.N.\r\nby an Institute of Science and Technology Austria (ISTA) GROW fellowship, and G.C. by an\r\nISTA Plus postdoctoral fellowship from the European Commission. S.H./L.B.S. and G.C. were\r\nadditionally supported by institutional funds from the ISTA and the University of Exeter,\r\nrespectively. ","type":"preprint","_id":"21291","title":"Lineage origin of spinal cord cell type diversity","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"has_accepted_license":"1"},{"oa":1,"month":"02","day":"16","pmid":1,"article_type":"original","ddc":["530"],"citation":{"chicago":"Rak, Dmytro, Dusan Lorenc, Daniel Balazs, Ayan A. Zhumekenov, Osman M. Bakr, and Zhanybek Alpichshev. “Flexoelectric Domain Walls Enable Charge Separation and Transport in Cubic Perovskites.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-68660-5\">https://doi.org/10.1038/s41467-026-68660-5</a>.","ama":"Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. Flexoelectric domain walls enable charge separation and transport in cubic perovskites. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-68660-5\">10.1038/s41467-026-68660-5</a>","ieee":"D. Rak, D. Lorenc, D. Balazs, A. A. Zhumekenov, O. M. Bakr, and Z. Alpichshev, “Flexoelectric domain walls enable charge separation and transport in cubic perovskites,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026.","mla":"Rak, Dmytro, et al. “Flexoelectric Domain Walls Enable Charge Separation and Transport in Cubic Perovskites.” <i>Nature Communications</i>, vol. 17, 946, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-68660-5\">10.1038/s41467-026-68660-5</a>.","short":"D. Rak, D. Lorenc, D. Balazs, A.A. Zhumekenov, O.M. Bakr, Z. Alpichshev, Nature Communications 17 (2026).","ista":"Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. 2026. Flexoelectric domain walls enable charge separation and transport in cubic perovskites. Nature Communications. 17, 946.","apa":"Rak, D., Lorenc, D., Balazs, D., Zhumekenov, A. A., Bakr, O. M., &#38; Alpichshev, Z. (2026). Flexoelectric domain walls enable charge separation and transport in cubic perovskites. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-68660-5\">https://doi.org/10.1038/s41467-026-68660-5</a>"},"abstract":[{"lang":"eng","text":"The exceptional energy-harvesting efficiency of lead-halide perovskites arises from unusually long photocarrier diffusion lengths and recombination lifetimes that persist even in defect-rich, solution-grown samples. Paradoxically, perovskites are also known for having very short exciton decay times. Here, we resolve this apparent contradiction by showing that key optoelectronic properties of perovskites can be explained by localized flexoelectric polarization confined to interfaces between domains of spontaneous strain. Using birefringence imaging, electrochemical staining, and zero-bias photocurrent measurements, we visualize the domain structure and directly probe the associated internal fields in nominally cubic single crystals of methylammonium lead bromide. We demonstrate that localized flexoelectric fields spatially separate electrons and holes to opposite sides of domain walls, exponentially suppressing recombination. Domain walls thus act as efficient mesoscopic transport channels for long-lived photocarriers, microscopically linking structural heterogeneity to charge transport and offering mechanistically informed design principles for perovskite solar-energy technologies."}],"article_number":"946","scopus_import":"1","article_processing_charge":"Yes","publication_status":"published","department":[{"_id":"ZhAl"},{"_id":"LifeSc"}],"OA_place":"publisher","file_date_updated":"2026-03-02T14:27:56Z","DOAJ_listed":"1","intvolume":"        17","publisher":"Springer Nature","PlanS_conform":"1","acknowledgement":"We are grateful to A. G. Volosniev for the valuable discussions. We thank D. Milius for the assistance with microscopy. D. R. would like to thank F. Filakovský and T. Čuchráč for the valuable discussions. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Miba Machine Shop Facility (MS).","volume":17,"type":"journal_article","title":"Flexoelectric domain walls enable charge separation and transport in cubic perovskites","_id":"21382","corr_author":"1","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","related_material":{"link":[{"url":"https://ista.ac.at/en/news/explaining-next-generation-solar-cells/","relation":"press_release","description":"News on ISTA website"}]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","oa_version":"Published Version","doi":"10.1038/s41467-026-68660-5","acknowledged_ssus":[{"_id":"Bio"},{"_id":"M-Shop"}],"quality_controlled":"1","language":[{"iso":"eng"}],"status":"public","date_updated":"2026-04-28T12:12:46Z","year":"2026","publication":"Nature Communications","publication_identifier":{"eissn":["2041-1723"]},"author":[{"full_name":"Rak, Dmytro","id":"70313b46-47c2-11ec-9e88-cd79101918fe","first_name":"Dmytro","last_name":"Rak"},{"id":"40D8A3E6-F248-11E8-B48F-1D18A9856A87","full_name":"Lorenc, Dusan","first_name":"Dusan","last_name":"Lorenc"},{"first_name":"Daniel","full_name":"Balazs, Daniel","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","last_name":"Balazs","orcid":"0000-0001-7597-043X"},{"last_name":"Zhumekenov","full_name":"Zhumekenov, Ayan A.","first_name":"Ayan A."},{"first_name":"Osman M.","last_name":"Bakr","full_name":"Bakr, Osman M."},{"orcid":"0000-0002-7183-5203","id":"45E67A2A-F248-11E8-B48F-1D18A9856A87","full_name":"Alpichshev, Zhanybek","last_name":"Alpichshev","first_name":"Zhanybek"}],"date_created":"2026-03-02T10:06:58Z","file":[{"success":1,"date_created":"2026-03-02T14:27:56Z","file_size":2570918,"file_name":"2026_NatureComm_Rak.pdf","creator":"dernst","relation":"main_file","checksum":"dd7a98de892d0b5abefca7e290ca0f77","file_id":"21390","content_type":"application/pdf","access_level":"open_access","date_updated":"2026-03-02T14:27:56Z"}],"OA_type":"gold","date_published":"2026-02-16T00:00:00Z","external_id":{"pmid":["41698893"]}},{"publisher":"Elsevier","intvolume":"        36","file_date_updated":"2026-03-24T08:34:37Z","publication_status":"published","department":[{"_id":"JiFr"}],"OA_place":"publisher","article_processing_charge":"Yes (via OA deal)","abstract":[{"lang":"eng","text":"Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development."}],"citation":{"apa":"Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., &#38; Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>","ista":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6.","short":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6.","mla":"Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>, vol. 36, no. 6, Elsevier, 2026, p. 1468–1480.e6, doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>.","chicago":"Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>.","ieee":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” <i>Current Biology</i>, vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026.","ama":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. 2026;36(6):1468-1480.e6. doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>"},"article_type":"original","ddc":["580"],"pmid":1,"page":"1468-1480.e6","day":"23","month":"03","oa":1,"external_id":{"pmid":["41831441"]},"OA_type":"hybrid","date_published":"2026-03-23T00:00:00Z","file":[{"file_id":"21496","content_type":"application/pdf","access_level":"open_access","date_updated":"2026-03-24T08:34:37Z","checksum":"fe6c41fdab58a55df5f2a5860c02acdc","file_size":12986894,"file_name":"2026_CurrentBiology_Li.pdf","relation":"main_file","creator":"dernst","success":1,"date_created":"2026-03-24T08:34:37Z"}],"date_created":"2026-03-23T15:11:16Z","publication_identifier":{"issn":["0960-9822"]},"publication":"Current Biology","author":[{"first_name":"Mingyue","last_name":"Li","id":"01f96916-0235-11eb-9379-a323192643b7","full_name":"Li, Mingyue"},{"first_name":"Nikola","full_name":"Rydza, Nikola","last_name":"Rydza"},{"first_name":"Ewa","last_name":"Mazur","full_name":"Mazur, Ewa"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","full_name":"Molnar, Gergely","last_name":"Molnar","first_name":"Gergely"},{"last_name":"Nodzyński","first_name":"Tomasz","full_name":"Nodzyński, Tomasz"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří"}],"date_updated":"2026-03-24T08:36:40Z","year":"2026","language":[{"iso":"eng"}],"status":"public","issue":"6","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"Bio"},{"_id":"LifeSc"}],"oa_version":"Published Version","doi":"10.1016/j.cub.2026.02.023","quality_controlled":"1","has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization","_id":"21490","type":"journal_article","acknowledgement":"We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.","volume":36,"project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"name":"Identification of a novel regulator in auxin canalization","_id":"bd906599-d553-11ed-ba76-abf8547645d7","grant_number":"E271"}],"PlanS_conform":"1"},{"has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","_id":"21746","title":"Multifold increase in spinal inhibitory cell types with emergence of limb movement","type":"journal_article","acknowledgement":"We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).","volume":45,"project":[{"grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814"},{"name":"Tools for automation and feedback microscopy","grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473"},{"grant_number":"FTI21-D-046","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"}],"PlanS_conform":"1","external_id":{"pmid":["41964955 "]},"date_published":"2026-04-28T00:00:00Z","OA_type":"gold","file":[{"file_size":14925958,"file_name":"2026_CellReports_Vijatovic.pdf","relation":"main_file","creator":"dernst","success":1,"date_created":"2026-05-04T12:20:10Z","file_id":"21795","access_level":"open_access","content_type":"application/pdf","date_updated":"2026-05-04T12:20:10Z","checksum":"0d26cdb5b8d8dec3a911d8261a65cdef"}],"date_created":"2026-04-19T22:07:43Z","author":[{"first_name":"David","last_name":"Vijatovic","full_name":"Vijatovic, David","id":"cf391e77-ec3c-11ea-a124-d69323410b58"},{"id":"2f73f876-f128-11eb-9611-b96b5a30cb0e","full_name":"Toma, Florina Alexandra ","last_name":"Toma","first_name":"Florina Alexandra "},{"first_name":"Y","full_name":"Ignatyev, Y","last_name":"Ignatyev"},{"first_name":"Zoe P","id":"a8144562-32c9-11ee-b5ce-d9800628bda2","last_name":"Harrington","full_name":"Harrington, Zoe P","orcid":"0009-0008-0158-4032"},{"orcid":"0000-0003-1216-9105","first_name":"Christoph M","full_name":"Sommer, Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert"},{"id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","full_name":"Smits, Matthijs Geert","first_name":"Matthijs Geert","last_name":"Smits"},{"full_name":"Dalla Vecchia, Marco","first_name":"Marco","last_name":"Dalla Vecchia","id":"02a7a869-ff06-11ed-a87f-86649d6077e5"},{"last_name":"Trevisan","full_name":"Trevisan, Alexandra J.","first_name":"Alexandra J."},{"full_name":"Chapman, Phillip","last_name":"Chapman","first_name":"Phillip"},{"full_name":"Julseth, Mara","last_name":"Julseth","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1","first_name":"Mara"},{"first_name":"Susan","full_name":"Brenner-Morton, Susan","last_name":"Brenner-Morton"},{"first_name":"Mariano I.","last_name":"Gabitto","full_name":"Gabitto, Mariano I."},{"last_name":"Dasen","full_name":"Dasen, Jeremy S.","first_name":"Jeremy S."},{"full_name":"Bikoff, Jay B.","first_name":"Jay B.","last_name":"Bikoff"},{"orcid":"0000-0001-9242-5601","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"}],"publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"publication":"Cell Reports","year":"2026","date_updated":"2026-05-04T12:27:06Z","status":"public","issue":"4","language":[{"iso":"eng"}],"quality_controlled":"1","oa_version":"Published Version","doi":"10.1016/j.celrep.2026.117227","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"citation":{"short":"D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).","apa":"Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>","ista":"Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227.","ama":"Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. 2026;45(4). doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>","chicago":"Vijatovic, David, Florina Alexandra  Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>.","ieee":"D. Vijatovic <i>et al.</i>, “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” <i>Cell Reports</i>, vol. 45, no. 4. Elsevier, 2026.","mla":"Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>, vol. 45, no. 4, 117227, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>."},"abstract":[{"lang":"eng","text":"As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution."}],"ddc":["570"],"article_type":"original","pmid":1,"day":"28","month":"04","oa":1,"publisher":"Elsevier","intvolume":"        45","DOAJ_listed":"1","file_date_updated":"2026-05-04T12:20:10Z","publication_status":"published","OA_place":"publisher","department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"article_processing_charge":"Yes","scopus_import":"1","article_number":"117227"},{"external_id":{"pmid":["41990175"]},"date_created":"2026-04-26T22:01:46Z","date_published":"2026-04-16T00:00:00Z","ec_funded":1,"OA_type":"closed access","date_updated":"2026-04-28T13:29:05Z","year":"2026","author":[{"orcid":"0000-0002-3461-5391","full_name":"Springstein, Benjamin L","id":"b4eb62ef-ac72-11ed-9503-ed3b4d66c083","first_name":"Benjamin L","last_name":"Springstein"},{"last_name":"Javoor","first_name":"Manjunath","id":"305ab18b-dc7d-11ea-9b2f-b58195228ea2","full_name":"Javoor, Manjunath","orcid":"0000-0003-2311-2112"},{"full_name":"Megrian, Daniela","last_name":"Megrian","first_name":"Daniela"},{"last_name":"Hajdu","full_name":"Hajdu, Roman","first_name":"Roman","id":"ffab949d-133f-11ed-8f02-94de21ace503"},{"full_name":"Hanke, Dustin M.","last_name":"Hanke","first_name":"Dustin M."},{"orcid":"0000-0002-9561-1239","last_name":"Zens","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","first_name":"Bettina","full_name":"Zens, Bettina"},{"first_name":"Gregor L.","full_name":"Weiss, Gregor L.","last_name":"Weiss"},{"orcid":"0000-0003-4790-8078","full_name":"Schur, Florian Km","first_name":"Florian Km","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Martin","last_name":"Loose","id":"462D4284-F248-11E8-B48F-1D18A9856A87","full_name":"Loose, Martin","orcid":"0000-0001-7309-9724"}],"publication_identifier":{"eissn":["1095-9203"],"issn":["0036-8075"]},"publication":"Science","quality_controlled":"1","doi":"10.1126/science.aea6343","acknowledged_ssus":[{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"oa_version":"None","issue":"6795","status":"public","language":[{"iso":"eng"}],"_id":"21762","title":"Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","type":"journal_article","volume":392,"acknowledgement":"We thank all members of the Loose lab at ISTA for helpful discussions; M. Kojic for critical reading of the manuscript; A. Herrero (Sevilla University) for sharing her extensive BACTH plasmid library and other plasmids, as well as cyanobacterial strains; T. Dagan and F. Nies (both Kiel University) for sharing cyanobacterial strains and plasmids and for valuable discussions; N. Sapay and A. Michon for providing the Amphipaseek code, which enabled us to perform our large-scale amphipathic helix screen of cyanobacterial CorR proteins; V.-V. Hodirnau for support in cryo-ET data collection; and J. Hansen for advice about cryo-EM data processing.\r\nThis work was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF), the Scientific Computing (SciComp), the Electron Microscopy Facility (EMF), and the Lab Support Facility (LSF). This work was funded by the European Union’s Horizon 2020 research and innovation program (Marie Skłodowska-Curie grant 101034413 to B.L.S.); the European Research Council (ERC) of the European Union (grant ActinID 101076260 to F.K.M.S.); the Swiss National Science Foundation (starting grant TMSGI3_226208 to G.L.W.); and the Jean-Jacques et Letitia Lopez-Loreta Foundation (G.L.W.).","project":[{"name":"IST-BRIDGE: International postdoctoral program","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413","call_identifier":"H2020"},{"grant_number":"101076260","_id":"bd980d18-d553-11ed-ba76-ceaa645c97eb","name":"A molecular atlas of Actin filament IDentities in the cell motility machinery"}],"intvolume":"       392","publisher":"AAAS","publication_status":"published","department":[{"_id":"MaLo"},{"_id":"FlSc"},{"_id":"GradSch"},{"_id":"EM-Fac"}],"scopus_import":"1","article_number":"eaea6343","article_processing_charge":"No","article_type":"original","abstract":[{"text":"Bacteria, like eukaryotes, use conserved cytoskeletal systems for intracellular organization. The plasmid-encoded ParMRC system forms actin-like filaments that segregate low–copy number plasmids. In multicellular cyanobacteria such as Anabaena sp., we found that a chromosomally encoded ParMR system has evolved into a cytoskeletal system named CorMR with a function in cell shape control rather than DNA segregation. Live-cell imaging, in vitro reconstitution, and cryo–electron microscopy revealed that CorM formed dynamically unstable, antiparallel double-stranded filaments that were recruited to the membrane by CorR through an amphipathic helix conserved in multicellular cyanobacteria. CorMR filaments were regulated by MinC, which excluded them from the poles and division plane. Comparative genomics indicated that the repurposing of ParMR and Min systems coevolved with cyanobacterial multicellularity, highlighting the evolutionary plasticity of cytoskeletal systems in bacteria.","lang":"eng"}],"citation":{"apa":"Springstein, B. L., Javoor, M., Megrian, D., Hajdu, R., Hanke, D. M., Zens, B., … Loose, M. (2026). Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.aea6343\">https://doi.org/10.1126/science.aea6343</a>","ista":"Springstein BL, Javoor M, Megrian D, Hajdu R, Hanke DM, Zens B, Weiss GL, Schur FK, Loose M. 2026. Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape. Science. 392(6795), eaea6343.","short":"B.L. Springstein, M. Javoor, D. Megrian, R. Hajdu, D.M. Hanke, B. Zens, G.L. Weiss, F.K. Schur, M. Loose, Science 392 (2026).","mla":"Springstein, Benjamin L., et al. “Repurposing of a DNA Segregation Machinery into a Cytoskeletal System Controlling Cell Shape.” <i>Science</i>, vol. 392, no. 6795, eaea6343, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/science.aea6343\">10.1126/science.aea6343</a>.","ama":"Springstein BL, Javoor M, Megrian D, et al. Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape. <i>Science</i>. 2026;392(6795). doi:<a href=\"https://doi.org/10.1126/science.aea6343\">10.1126/science.aea6343</a>","ieee":"B. L. Springstein <i>et al.</i>, “Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape,” <i>Science</i>, vol. 392, no. 6795. AAAS, 2026.","chicago":"Springstein, Benjamin L, Manjunath Javoor, Daniela Megrian, Roman Hajdu, Dustin M. Hanke, Bettina Zens, Gregor L. Weiss, Florian KM Schur, and Martin Loose. “Repurposing of a DNA Segregation Machinery into a Cytoskeletal System Controlling Cell Shape.” <i>Science</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/science.aea6343\">https://doi.org/10.1126/science.aea6343</a>."},"day":"16","pmid":1,"month":"04"},{"publisher":"AAAS","intvolume":"       392","article_processing_charge":"No","scopus_import":"1","file_date_updated":"2026-05-07T05:54:43Z","OA_place":"repository","publication_status":"published","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"page":"296-300","pmid":1,"day":"16","abstract":[{"lang":"eng","text":"Reactive oxygen species (ROS) have been implicated in multiple signaling processes in plants, but the underlying mechanisms and roles remain enigmatic. In this study, we developed a method of live imaging of apoplastic ROS at the root surface. Distinct signals, including auxin, extracellular adenosine triphosphate, and rapid alkalinization factor 1 peptide, induce cytosolic calcium transients and apoplastic ROS bursts. Genetic and optogenetic manipulations of Arabidopsis identified calcium transients as necessary and sufficient for ROS bursts through activation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases RBOHC and RBOHF. Apoplastic ROS bursts are not required, but they do limit gravity-induced root bending. Root bending is sensed by the stretch-activated calcium channel MCA1, leading to NADPH oxidase activation. The resulting ROS production stiffens cell walls to facilitate soil penetration. Apoplastic ROS thus provides a means to balance tissue flexibility and stiffness to navigate soil."}],"citation":{"short":"I. Kulich, D. Vladimirtsev, M. Randuch, S. Gao, M. Citterico, K.R. Konrad, G. Nagel, M. Wrzaczek, L. Cascaro, P. Vinet, P. Durand, A. Asnacios, L. Verma, M.J. Bennett, B.K. Pandey, J. Friml, Science 392 (2026) 296–300.","ista":"Kulich I, Vladimirtsev D, Randuch M, Gao S, Citterico M, Konrad KR, Nagel G, Wrzaczek M, Cascaro L, Vinet P, Durand P, Asnacios A, Verma L, Bennett MJ, Pandey BK, Friml J. 2026. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 392(6795), 296–300.","apa":"Kulich, I., Vladimirtsev, D., Randuch, M., Gao, S., Citterico, M., Konrad, K. R., … Friml, J. (2026). Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>","mla":"Kulich, Ivan, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>, vol. 392, no. 6795, AAAS, 2026, pp. 296–300, doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>.","ieee":"I. Kulich <i>et al.</i>, “Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation,” <i>Science</i>, vol. 392, no. 6795. AAAS, pp. 296–300, 2026.","ama":"Kulich I, Vladimirtsev D, Randuch M, et al. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. 2026;392(6795):296-300. doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>","chicago":"Kulich, Ivan, Dmitrii Vladimirtsev, Marek Randuch, Shiqiang Gao, Matteo Citterico, Kai R. Konrad, Georg Nagel, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>."},"ddc":["580"],"article_type":"original","oa":1,"month":"04","date_published":"2026-04-16T00:00:00Z","OA_type":"green","file":[{"date_created":"2026-05-07T05:54:43Z","success":1,"file_size":6150733,"file_name":"2026_Science_Kulich_accepted.pdf","creator":"dernst","relation":"main_file","checksum":"eb5b29247832ecdc53c8146da0509bbe","content_type":"application/pdf","access_level":"open_access","date_updated":"2026-05-07T05:54:43Z","file_id":"21832"}],"date_created":"2026-04-26T22:01:47Z","external_id":{"pmid":["41990180"]},"issue":"6795","status":"public","language":[{"iso":"eng"}],"quality_controlled":"1","doi":"10.1126/science.adu8197","oa_version":"Accepted Version","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"author":[{"id":"57a1567c-8314-11eb-9063-c9ddc3451a54","full_name":"Kulich, Ivan","last_name":"Kulich","first_name":"Ivan"},{"full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii","last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d"},{"full_name":"Randuch, Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","first_name":"Marek"},{"first_name":"Shiqiang","full_name":"Gao, Shiqiang","last_name":"Gao"},{"last_name":"Citterico","first_name":"Matteo","full_name":"Citterico, Matteo"},{"first_name":"Kai R.","full_name":"Konrad, Kai R.","last_name":"Konrad"},{"full_name":"Nagel, Georg","first_name":"Georg","last_name":"Nagel"},{"last_name":"Wrzaczek","first_name":"Michael","full_name":"Wrzaczek, Michael"},{"full_name":"Cascaro, Léa","first_name":"Léa","last_name":"Cascaro"},{"full_name":"Vinet, Pauline","first_name":"Pauline","last_name":"Vinet"},{"last_name":"Durand","full_name":"Durand, Pauline","first_name":"Pauline"},{"full_name":"Asnacios, Atef","first_name":"Atef","last_name":"Asnacios"},{"first_name":"Lokesh","last_name":"Verma","full_name":"Verma, Lokesh"},{"last_name":"Bennett","full_name":"Bennett, Malcolm J.","first_name":"Malcolm J."},{"last_name":"Pandey","full_name":"Pandey, Bipin K.","first_name":"Bipin K."},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"publication":"Science","publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"date_updated":"2026-05-07T06:20:07Z","year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","_id":"21763","title":"Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation","has_accepted_license":"1","project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"type":"journal_article","acknowledgement":"We gratefully acknowledge the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) (both of ISTA) and the Hounsfield CT Facility (University of Nottingham) for support with imaging and the Growth Facility (IPMB) for plant cultivation. We thank M. Fendrych and his team for help with the microfluidics upgrades and J. Atkinson at the University of Nottingham MakerSpace for 3D printing of Arabidopsis mini-soil columns.\r\nThis project received funding from the European Research Council (ERC; 101142681 CYNIPS) and the Austrian Science Fund (FWF; P 37051-B). I.K. was cofunded by the European Union, Horizon Europe, project MOLIPEC, ID 101087030 and CSF project 25-16449S. L.V. and B.K.P. acknowledge funding from UK Research and Innovation (UKRI) Frontiers Research (EP/Y036697/1). M.J.B. acknowledges funding from ERC SYNERGY (grant 101118769 HYDROSENSING). The study was partially supported by the Université Paris Cité, Idex ANR-18-IDEX-0001, funded by the French Government through its “Investments for the Future” program and also by the projects “Mecha-Nuc” ANR-20-CE13-0025-03 and “scEm-bryoMech” ANR-21-CE13-0046. P.D. acknowledges support by Human Frontier Science Program Organization grant 2022-RG107. P.V. acknowledges support provided by “Programme blanc” of the Graduate School BIOSPHERA, Université Paris-Saclay. Phytohormonal analysis was performed using the service laboratory funded by Toward Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the European Regional Development Fund (ERDF) program Johannes Amos Comenius. This research was funded in whole or in part by the Austrian Science Fund (P 37051-B) and UK Research and Innovation (EP/Y036697/1), cOAlition S organizations, and by the European Research Council (101142681 CYNIPS, 101118769 HYDROSENSING); as required, the author will make the Author Accepted Manuscript (AAM) version available under a CC BY public copyright license.","volume":392},{"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)"},"has_accepted_license":"1","_id":"21962","title":"Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"We thank A. Heger (IST Austria Preclinical Facility), A. Sommer (VBCF GmbH, NGS Unit), and A.\r\nNicolas (IST Austria Lab Support Facility / Mass Spectrometry Facility) for technical support; K. Ferencak,\r\nI. Aykara, P. Hirschfeld, E. Fisher, S. Laukoter, L. Andersen for initial experiments and/or assistance; and\r\nall members of the Hippenmeyer lab for discussion. This research was supported by the Scientific Service\r\nUnits (SSU) of IST Austria through resources provided by the Imaging and Optics- (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). R.B. received support from FWF Meitner-Programm (M 2416). This\r\nwork was also supported by IST Austria institutional funds; the People Programme (Marie Curie Actions)\r\nof the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement\r\nNo 618444 to S.H., and the European Research Council (ERC) under the European Union’s Horizon 2020\r\nresearch and innovation programme (grant agreement No 725780 LinPro) to S.H.","type":"preprint","project":[{"call_identifier":"FWF","name":"Molecular Mechanisms Regulating Gliogenesis in the Neocortex","_id":"264E56E2-B435-11E9-9278-68D0E5697425","grant_number":"M02416"},{"_id":"25D61E48-B435-11E9-9278-68D0E5697425","grant_number":"618444","name":"Molecular Mechanisms of Cerebral Cortex Development","call_identifier":"FP7"},{"name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","_id":"260018B0-B435-11E9-9278-68D0E5697425","grant_number":"725780","call_identifier":"H2020"}],"date_created":"2026-06-09T08:08:18Z","date_published":"2026-05-05T00:00:00Z","ec_funded":1,"OA_type":"green","date_updated":"2026-06-16T08:45:25Z","year":"2026","author":[{"first_name":"Ana","last_name":"Villalba Requena","full_name":"Villalba Requena, Ana","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","orcid":"0000-0002-5615-5277"},{"id":"2E26DF60-F248-11E8-B48F-1D18A9856A87","full_name":"Beattie, Robert J","last_name":"Beattie","first_name":"Robert J","orcid":"0000-0002-8483-8753"},{"orcid":"0000-0002-7462-0048","id":"48EA0138-F248-11E8-B48F-1D18A9856A87","full_name":"Pauler, Florian","last_name":"Pauler","first_name":"Florian"},{"last_name":"Streicher","first_name":"Carmen","full_name":"Streicher, Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-6618-6889","full_name":"Miranda, Osvaldo","last_name":"Miranda","first_name":"Osvaldo","id":"862A3C56-A8BF-11E9-B4FA-D9E3E5697425"},{"last_name":"Krausgruber","first_name":"Thomas","full_name":"Krausgruber, Thomas"},{"last_name":"Senekowitsch","full_name":"Senekowitsch, Martin","first_name":"Martin"},{"last_name":"Farlik","first_name":"Matthias","full_name":"Farlik, Matthias"},{"last_name":"Bock","full_name":"Bock, Christoph","first_name":"Christoph"},{"last_name":"Rülicke","full_name":"Rülicke, Thomas","first_name":"Thomas"},{"last_name":"Hippenmeyer","full_name":"Hippenmeyer, Simon","first_name":"Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2279-1061"}],"publication":"bioRxiv","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"MassSpec"},{"_id":"Bio"}],"oa_version":"Preprint","doi":"10.64898/2026.05.01.722172","status":"public","language":[{"iso":"eng"}],"ddc":["570"],"citation":{"chicago":"Villalba Requena, Ana, Robert J Beattie, Florian Pauler, Carmen Streicher, Osvaldo Miranda, Thomas Krausgruber, Martin Senekowitsch, et al. “Mtor/Rptor Function Globally Prevents Cortical Microcephaly and Cell-Autonomously Promotes Postnatal Neuron Survival in Cell Type Specific Manner.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.05.01.722172\">https://doi.org/10.64898/2026.05.01.722172</a>.","ama":"Villalba Requena A, Beattie RJ, Pauler F, et al. Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>","ieee":"A. Villalba Requena <i>et al.</i>, “Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner,” <i>bioRxiv</i>. .","mla":"Villalba Requena, Ana, et al. “Mtor/Rptor Function Globally Prevents Cortical Microcephaly and Cell-Autonomously Promotes Postnatal Neuron Survival in Cell Type Specific Manner.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>.","short":"A. Villalba Requena, R.J. Beattie, F. Pauler, C. Streicher, O. Miranda, T. Krausgruber, M. Senekowitsch, M. Farlik, C. Bock, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.).","ista":"Villalba Requena A, Beattie RJ, Pauler F, Streicher C, Miranda O, Krausgruber T, Senekowitsch M, Farlik M, Bock C, Rülicke T, Hippenmeyer S. Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. bioRxiv, <a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>.","apa":"Villalba Requena, A., Beattie, R. J., Pauler, F., Streicher, C., Miranda, O., Krausgruber, T., … Hippenmeyer, S. (n.d.). Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.05.01.722172\">https://doi.org/10.64898/2026.05.01.722172</a>"},"abstract":[{"text":"The generation of faithful cell-type diversity and correct projection neuron numbers is essential for cerebral cortex development. Corticogenesis is however susceptible to genetic interference of critical signaling pathways, including mutations in Mtor/Rptor that lead to microcephaly. How the loss of Rptor/mTORC1 function affects cortical developmental programs, at single cell level, is still unknown. Here, we utilized Mosaic Analysis with Double Markers (MADM) technology to probe Rptor gene function upon sparse single cell- or global tissue-wide ablation. We found that tissue-wide effects drive the etiology of cortical microcephaly upon loss of Rptor, rather than deficits in projection neuron genesis. Conversely, Rptor function is cell-autonomously required for postnatal projection neuron survival in a highly cell-type-specific manner. Collectively, our results suggest that the fine balance of precise cell-type-specific cell-autonomous Rptor/mTORC1 function in concert with non-cell-autonomous tissue-wide effects is essential for the development of a properly-sized cerebral cortex with accurate projection neuron diversity.","lang":"eng"}],"day":"05","month":"05","main_file_link":[{"open_access":"1","url":"https://doi.org/10.64898/2026.05.01.722172"}],"oa":1,"publication_status":"submitted","OA_place":"repository","department":[{"_id":"SiHi"}],"article_processing_charge":"No"},{"date_created":"2026-06-09T08:08:53Z","ec_funded":1,"OA_type":"green","date_published":"2026-05-05T00:00:00Z","oa_version":"Preprint","doi":"10.64898/2026.05.01.722191","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"language":[{"iso":"eng"}],"status":"public","year":"2026","date_updated":"2026-06-16T08:57:20Z","publication":"bioRxiv","author":[{"orcid":"0000-0001-6618-6889","full_name":"Miranda, Osvaldo","id":"862A3C56-A8BF-11E9-B4FA-D9E3E5697425","first_name":"Osvaldo","last_name":"Miranda"},{"last_name":"Contreras","first_name":"Ximena","full_name":"Contreras, Ximena","id":"475990FE-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Pauler, Florian","last_name":"Pauler","first_name":"Florian","id":"48EA0138-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7462-0048"},{"full_name":"Davaatseren, Amarbayasgalan","first_name":"Amarbayasgalan","id":"70ADC922-B424-11E9-99E3-BA18E6697425","last_name":"Davaatseren"},{"orcid":"0000-0002-3183-8207","last_name":"Amberg","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","full_name":"Amberg, Nicole","first_name":"Nicole"},{"full_name":"Streicher, Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher","first_name":"Carmen"},{"orcid":"0000-0002-5615-5277","first_name":"Ana","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","full_name":"Villalba Requena, Ana","last_name":"Villalba Requena"},{"full_name":"Heger, Anna-Magdalena","id":"4B76FFD2-F248-11E8-B48F-1D18A9856A87","last_name":"Heger","first_name":"Anna-Magdalena"},{"full_name":"Marie, Corentine","first_name":"Corentine","last_name":"Marie"},{"first_name":"Bassem A.","full_name":"Hassan, Bassem A.","last_name":"Hassan"},{"last_name":"Rülicke","first_name":"Thomas","full_name":"Rülicke, Thomas"},{"id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","last_name":"Hippenmeyer","first_name":"Simon","orcid":"0000-0003-2279-1061"}],"title":"Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production","_id":"21963","corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)"},"has_accepted_license":"1","project":[{"_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","grant_number":"F7805","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression"},{"grant_number":"725780","_id":"260018B0-B435-11E9-9278-68D0E5697425","name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","call_identifier":"H2020"}],"type":"preprint","acknowledgement":"We thank Kay-Uwe Wagner (Wayne State University) for generously sharing Jak1/2–flox mouse lines; A.\r\nSommer (VBCF GmbH, NGS Unit) for technical support; N. Kim, V. Mick, S. Schnabl, S. Gobeil, and L.\r\nAndersen for technical assistance; all members of the Hippenmeyer lab for discussion and B. Novitch for\r\ncomments on earlier versions of the manuscript. This research was supported by the Scientific Service Units\r\n(SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). O.A.M received support from the Austrian Academy of Sciences\r\nÖAW (DOC 186584), and N.A. from FWF Elise Richter Program (Grant V1041T). This work was also\r\nsupported by IST Austria institutional funds; FWF SFB F78 (Neuro Stem Modulation) to S.H., and the\r\nEuropean Research Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogramme (grant agreement No 725780 LinPro) to S.H.","article_processing_charge":"No","department":[{"_id":"SiHi"},{"_id":"PreCl"},{"_id":"GradSch"}],"publication_status":"submitted","OA_place":"repository","day":"05","ddc":["570"],"citation":{"apa":"Miranda, O., Contreras, X., Pauler, F., Davaatseren, A., Amberg, N., Streicher, C., … Hippenmeyer, S. (n.d.). Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.05.01.722191\">https://doi.org/10.64898/2026.05.01.722191</a>","ista":"Miranda O, Contreras X, Pauler F, Davaatseren A, Amberg N, Streicher C, Villalba Requena A, Heger A-M, Marie C, Hassan BA, Rülicke T, Hippenmeyer S. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv, <a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>.","short":"O. Miranda, X. Contreras, F. Pauler, A. Davaatseren, N. Amberg, C. Streicher, A. Villalba Requena, A.-M. Heger, C. Marie, B.A. Hassan, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.).","ama":"Miranda O, Contreras X, Pauler F, et al. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>","ieee":"O. Miranda <i>et al.</i>, “Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production,” <i>bioRxiv</i>. .","chicago":"Miranda, Osvaldo, Ximena Contreras, Florian Pauler, Amarbayasgalan Davaatseren, Nicole Amberg, Carmen Streicher, Ana Villalba Requena, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.05.01.722191\">https://doi.org/10.64898/2026.05.01.722191</a>.","mla":"Miranda, Osvaldo, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>."},"abstract":[{"text":"The cerebral cortex consists of immense numbers of neuronal and glial cell-types derived from radial glial progenitor (RGP) cells. How RGPs generate appropriate quantities of distinct cortical cell-types to safeguard a brain of correct size, is not well understood. However, genetic aberration in human, including mutations in PTEN, lead to cortical malformation such as macrocephaly, albeit with unknown etiology. Here we utilized Mosaic Analysis with Double Markers (MADM)-based clonal analysis and single cell phenotyping to decipher the role of Pten in neurogenic and gliogenic RGP lineage progression during cortical ontogeny. While neurogenic RGP lineage progression and projection neuron production was moderately altered in the absence of Pten, cortical astrocyte production was drastically increased. Through genetic epistasis experiments we show that the loss of Pten uncouples astrocyte generation from essential growth factor signaling hubs, funneling into MAPK. Collectively, our results suggest that Pten regulates RGP lineage progression with distinct sequential functions in cortical projection neurogenesis and astrocyte production to ensure the emergence of a correctly-sized cerebral cortex.","lang":"eng"}],"oa":1,"month":"05","main_file_link":[{"open_access":"1","url":"https://doi.org/10.64898/2026.05.01.722191"}]},{"scopus_import":"1","article_number":"5540","article_processing_charge":"Yes","publication_status":"published","OA_place":"publisher","department":[{"_id":"PeJo"},{"_id":"ScienComp"}],"file_date_updated":"2026-07-01T06:46:06Z","researchdata_availability":"yes","DOAJ_listed":"1","das_tickbox":"1","intvolume":"        17","publisher":"Springer Nature","oa":1,"month":"06","supplementarymaterial":"yes","day":"23","pmid":1,"ddc":["570"],"article_type":"original","abstract":[{"text":"Hippocampal CA3 pyramidal neurons (PNs) form the largest autoassociative network in the mammalian brain. Whether CA3–CA3 recurrent connectivity is genetically preconfigured or environmentally shaped during ongoing memory storage is currently unknown. To address this question, we performed multicellular patch-clamp-based circuit mapping of up to eight CA3 PNs in the mouse hippocampus at multiple postnatal time points (P7–8, P18–25, and P45–50). Here, we show that the hippocampal CA3 network undergoes a developmental transformation from local, dense, and random connectivity to a distributed, sparse, and structured configuration. Thus, sparse and structured connectivity may emerge via experience-dependent mechanisms. In parallel, the strength of single synapses is downregulated; single synaptic events are sufficient to trigger postsynaptic spiking early in development, whereas spatial summation of several inputs is required at later time points. Biologically inspired models of memory storage by Hebbian synaptic plasticity and retrieval via pattern completion suggest that developmental changes improve specific aspects of memory storage and retrieval. Our results imply a developmental transformation of the neuronal code and the memory functions in the hippocampal CA3 network.</jats:p>","lang":"eng"}],"citation":{"ieee":"V. M. Vargas Barroso, J. Watson, A. C. Navas Olivé, A. Schlögl, and P. M. Jonas, “Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026.","ama":"Vargas Barroso VM, Watson J, Navas Olivé AC, Schlögl A, Jonas PM. Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-71914-x\">10.1038/s41467-026-71914-x</a>","chicago":"Vargas Barroso, Victor M, Jake Watson, Andrea C Navas Olivé, Alois Schlögl, and Peter M Jonas. “Developmental Emergence of Sparse and Structured Synaptic Connectivity in the Hippocampal CA3 Memory Circuit.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-71914-x\">https://doi.org/10.1038/s41467-026-71914-x</a>.","mla":"Vargas Barroso, Victor M., et al. “Developmental Emergence of Sparse and Structured Synaptic Connectivity in the Hippocampal CA3 Memory Circuit.” <i>Nature Communications</i>, vol. 17, 5540, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-71914-x\">10.1038/s41467-026-71914-x</a>.","apa":"Vargas Barroso, V. M., Watson, J., Navas Olivé, A. C., Schlögl, A., &#38; Jonas, P. M. (2026). Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-71914-x\">https://doi.org/10.1038/s41467-026-71914-x</a>","ista":"Vargas Barroso VM, Watson J, Navas Olivé AC, Schlögl A, Jonas PM. 2026. Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. Nature Communications. 17, 5540.","short":"V.M. Vargas Barroso, J. Watson, A.C. Navas Olivé, A. Schlögl, P.M. Jonas, Nature Communications 17 (2026)."},"quality_controlled":"1","oa_version":"Published Version","doi":"10.1038/s41467-026-71914-x","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"M-Shop"},{"_id":"ScienComp"}],"status":"public","language":[{"iso":"eng"}],"year":"2026","date_updated":"2026-07-01T06:47:49Z","author":[{"first_name":"Victor M","full_name":"Vargas Barroso, Victor M","last_name":"Vargas Barroso","id":"2F55A9DE-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-8698-3823","id":"63836096-4690-11EA-BD4E-32803DDC885E","last_name":"Watson","first_name":"Jake","full_name":"Watson, Jake"},{"id":"739d26c9-52e8-11ee-8d72-f14d3893b4ce","first_name":"Andrea C","full_name":"Navas Olivé, Andrea C","last_name":"Navas Olivé","orcid":"0000-0002-9280-8597"},{"id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","first_name":"Alois","last_name":"Schlögl","full_name":"Schlögl, Alois","orcid":"0000-0002-5621-8100"},{"last_name":"Jonas","full_name":"Jonas, Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M","orcid":"0000-0001-5001-4804"}],"publication":"Nature Communications","publication_identifier":{"eissn":["2041-1723"]},"date_created":"2026-06-30T13:05:52Z","file":[{"date_created":"2026-07-01T06:46:06Z","success":1,"creator":"dernst","relation":"main_file","file_name":"2026_NatureComm_VargasBarroso.pdf","file_size":18304997,"checksum":"d0b0093493926985b4c268662ff4d556","date_updated":"2026-07-01T06:46:06Z","access_level":"open_access","content_type":"application/pdf","file_id":"22231"}],"date_published":"2026-06-23T00:00:00Z","ec_funded":1,"OA_type":"gold","external_id":{"pmid":["42014695"]},"PlanS_conform":"1","project":[{"call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse"},{"name":"Synaptic mechanisms of engram storage and retrieval in CA3 hippocampal microcircuits","grant_number":"101199096","_id":"e62b56fe-ab3c-11f0-94c7-d181dd352b3b"},{"call_identifier":"H2020","_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships"},{"grant_number":"101026635","_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","name":"Synaptic computations of the hippocampal CA3 circuitry","call_identifier":"H2020"},{"_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","grant_number":"P36232","name":"Mechanisms of GABA release in hippocampal circuits"},{"grant_number":"PAT 4178023","_id":"8d9195e9-16d5-11f0-9cad-d075be887a1e","name":"Synaptic networks of human brain"},{"name":"Reglas de Conectividad funcional en el hipocampo","_id":"26366136-B435-11E9-9278-68D0E5697425"}],"dataavailabilitystatement":"Source data are provided with this paper. Additional original data are available from the corresponding author upon request. Code is available from https://doi.org/10.15479/AT-ISTA-21442 under the link https://research-explorer.ista.ac.at/download/21442/21443/ca3simu-vargas2026v1.tar.gz","acknowledgement":"We thank Jose Guzman, Simon Hippenmeyer, and Tim Vogels for critically reading the manuscript, Jozsef Csicsvari for useful discussions, Florian Marr for technical assistance, and Eleftheria Kralli-Beller for manuscript editing. This research was supported by the Scientific Services Units (SSUs) of ISTA: the preclinical facility (PCF) provided housing and breeding of the animals, the imaging and optics facility (IOF) offered technical training and state of the art equipment, the Miba machine shop contributed to the construction and maintenance of multicellular recording setups, and the scientific computing unit helped with the large-scale simulations. The project received funding from the European Union’s Horizon 2020 research and innovation programme (ERC Advanced Grants No 692692 GIANTSYN and 101199096 CA3-SYNGRAM to P.J.; Marie Skłodowska-Curie Grant 754411 to V.V.B.; Marie Skłodowska-Curie Grant 101026635 to J.F.W.), the Fond zur Förderung der Wissenschaftlichen Forschung (P 36232-B, PAT4178023, and 10.55776/CoE16 to P.J.), and the Nomis Foundation (fellowship to A.N.-O.). V.V.B. received funding from a CONACyT fellowship (289638).","type":"journal_article","volume":17,"_id":"22229","title":"Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"related_material":{"record":[{"relation":"research_data","id":"21442","status":"public"}]},"has_accepted_license":"1"},{"publisher":"Institute of Science and Technology Austria","article_processing_charge":"No","file_date_updated":"2026-03-11T20:52:39Z","department":[{"_id":"GradSch"},{"_id":"EdHa"}],"OA_place":"repository","day":"12","citation":{"mla":"Dunajova, Zuzana. <i>Supplementary Movies to PhD Thesis “Geometry-Driven Self-Organization of Migrating Cells and Chiral Filaments.”</i> Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21439\">10.15479/AT-ISTA-21439</a>.","ieee":"Z. Dunajova, “Supplementary movies to PhD thesis ‘Geometry-driven self-organization of migrating cells and chiral filaments.’” Institute of Science and Technology Austria, 2026.","chicago":"Dunajova, Zuzana. “Supplementary Movies to PhD Thesis ‘Geometry-Driven Self-Organization of Migrating Cells and Chiral Filaments.’” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-21439\">https://doi.org/10.15479/AT-ISTA-21439</a>.","ama":"Dunajova Z. Supplementary movies to PhD thesis “Geometry-driven self-organization of migrating cells and chiral filaments.” 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21439\">10.15479/AT-ISTA-21439</a>","apa":"Dunajova, Z. (2026). Supplementary movies to PhD thesis “Geometry-driven self-organization of migrating cells and chiral filaments.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-21439\">https://doi.org/10.15479/AT-ISTA-21439</a>","ista":"Dunajova Z. 2026. Supplementary movies to PhD thesis “Geometry-driven self-organization of migrating cells and chiral filaments”, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT-ISTA-21439\">10.15479/AT-ISTA-21439</a>.","short":"Z. Dunajova, (2026)."},"abstract":[{"text":"These files contain supplementary movies accompanying the PhD thesis “Geometry-driven self-organization of migrating cells and chiral filaments” by Zuzana Dunajova (2026). The videos provide additional visual material supporting the experiments and results described in the thesis.","lang":"eng"}],"ddc":["570"],"contributor":[{"contributor_type":"researcher","first_name":"Saren","last_name":"Tasciyan","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1671-393X"},{"first_name":"Philipp","id":"40136C2A-F248-11E8-B48F-1D18A9856A87","last_name":"Radler","contributor_type":"researcher","orcid":"0000-0001-9198-2182 "}],"oa":1,"month":"03","date_published":"2026-03-12T00:00:00Z","OA_type":"free access","date_created":"2026-03-11T21:05:20Z","file":[{"file_size":154465214,"file_name":"Supplementary_movies_Thesis_Dunajova.zip","creator":"zdunajov","relation":"main_file","success":1,"date_created":"2026-03-11T20:41:28Z","file_id":"21440","access_level":"open_access","content_type":"application/zip","date_updated":"2026-03-11T20:41:28Z","checksum":"47809a9a31b748b16e21e92d11ddc87f"},{"access_level":"open_access","content_type":"text/plain","date_updated":"2026-03-11T20:52:39Z","file_id":"21441","checksum":"a64a174bc6abf0a5e77631e4fd121f1f","file_name":"readme.txt","file_size":2289,"creator":"zdunajov","relation":"main_file","date_created":"2026-03-11T20:52:39Z","success":1}],"license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","status":"public","doi":"10.15479/AT-ISTA-21439","acknowledged_ssus":[{"_id":"Bio"},{"_id":"ScienComp"}],"oa_version":"Published Version","author":[{"id":"4B39F286-F248-11E8-B48F-1D18A9856A87","last_name":"Dunajova","full_name":"Dunajova, Zuzana","first_name":"Zuzana"}],"year":"2026","date_updated":"2026-07-06T12:38:16Z","user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","corr_author":"1","_id":"21439","title":"Supplementary movies to PhD thesis “Geometry-driven self-organization of migrating cells and chiral filaments”","has_accepted_license":"1","tmp":{"short":"CC BY-NC-SA (4.0)","image":"/images/cc_by_nc_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)"},"related_material":{"record":[{"relation":"used_in_publication","id":"13314","status":"public"},{"id":"21423","relation":"used_in_publication","status":"public"},{"id":"21427","relation":"used_in_publication","status":"public"}]},"project":[{"name":"Motile active matter models of migrating cells and chiral filaments","_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","grant_number":"26360"}],"type":"research_data"},{"pmid":1,"day":"28","abstract":[{"text":"In many species, sex-biased expression is widespread and thought to contribute to sexual dimorphism. While bulk RNA-sequencing has been instrumental in identifying strongly sex-biased genes, it lacks resolution to assess variation across cell-types and tissue compartments. Using single-nucleus expression data from the Fly Cell Atlas, we investigate sex differences in adult Drosophila melanogaster. We find that differences in cell-type composition between the sexes are not a major source of sex-bias, as for the vast majority of genes, the degree of sex-bias is similar regardless of whether sex differences in cell-type composition are controlled for or not. Our analysis confirms a deficit of X-linked male-biased genes in the body’s somatic tissues that is widespread across cell-types. We also find the excess of X-linked female-biased genes to be associated with nervous system cells in the head but with epithelial cells in the body’s somatic tissues, showing that single-nucleus data crucially resolves sex-bias at the cell-type level. We investigate dosage compensation (DC) across 15 tissues and 17 cell-types. We observe that it varies throughout the body. Surprisingly, we observe a lack of DC in a cluster of main cells within the male accessory glands. This result highlights the importance of understanding context-dependent DC.","lang":"eng"}],"citation":{"mla":"de Castro Barbosa Rodrigues Barata, Carolina, and Beatriz Vicoso. “Single-Nucleus Resolution of Sex-Biased Expression and Dosage Compensation in Drosophila Melanogaster.” <i>Proceedings of the Royal Society B Biological Sciences</i>, vol. 293, no. 2063, 20252471, Royal Society of London, 2026, doi:<a href=\"https://doi.org/10.1098/rspb.2025.2471\">10.1098/rspb.2025.2471</a>.","ieee":"C. de Castro Barbosa Rodrigues Barata and B. Vicoso, “Single-nucleus resolution of sex-biased expression and dosage compensation in Drosophila melanogaster,” <i>Proceedings of the Royal Society B Biological Sciences</i>, vol. 293, no. 2063. Royal Society of London, 2026.","ama":"de Castro Barbosa Rodrigues Barata C, Vicoso B. Single-nucleus resolution of sex-biased expression and dosage compensation in Drosophila melanogaster. <i>Proceedings of the Royal Society B Biological Sciences</i>. 2026;293(2063). doi:<a href=\"https://doi.org/10.1098/rspb.2025.2471\">10.1098/rspb.2025.2471</a>","chicago":"Castro Barbosa Rodrigues Barata, Carolina de, and Beatriz Vicoso. “Single-Nucleus Resolution of Sex-Biased Expression and Dosage Compensation in Drosophila Melanogaster.” <i>Proceedings of the Royal Society B Biological Sciences</i>. Royal Society of London, 2026. <a href=\"https://doi.org/10.1098/rspb.2025.2471\">https://doi.org/10.1098/rspb.2025.2471</a>.","short":"C. de Castro Barbosa Rodrigues Barata, B. Vicoso, Proceedings of the Royal Society B Biological Sciences 293 (2026).","apa":"de Castro Barbosa Rodrigues Barata, C., &#38; Vicoso, B. (2026). Single-nucleus resolution of sex-biased expression and dosage compensation in Drosophila melanogaster. <i>Proceedings of the Royal Society B Biological Sciences</i>. Royal Society of London. <a href=\"https://doi.org/10.1098/rspb.2025.2471\">https://doi.org/10.1098/rspb.2025.2471</a>","ista":"de Castro Barbosa Rodrigues Barata C, Vicoso B. 2026. Single-nucleus resolution of sex-biased expression and dosage compensation in Drosophila melanogaster. Proceedings of the Royal Society B Biological Sciences. 293(2063), 20252471."},"ddc":["570"],"article_type":"original","oa":1,"month":"01","das_tickbox":"1","publisher":"Royal Society of London","intvolume":"       293","article_processing_charge":"Yes (via OA deal)","scopus_import":"1","article_number":"20252471","file_date_updated":"2026-02-16T09:26:02Z","department":[{"_id":"BeVi"}],"OA_place":"publisher","publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","_id":"21161","title":"Single-nucleus resolution of sex-biased expression and dosage compensation in Drosophila melanogaster","has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"project":[{"_id":"90ef7108-16d5-11f0-9cad-e6e116913473","grant_number":"ESP 6331524","name":"Does genetic drift set a limit on the adaptive evolution of sex-biased expression?"}],"PlanS_conform":"1","type":"journal_article","volume":293,"acknowledgement":"This work was partly funded by an Austrian Science Foundation FWF ESPRIT fellowship (10.55776/ESP6331524) to C.B. We would like to thank the Vicoso group for their invaluable input and discussions throughout this work. We thank Filip Ruzicka for his insightful comments on the manuscript. All computational resources were provided by the Scientific Computing Unit at ISTA. This research was also supported through resources provided by the Imaging & Optics Facility (IOF) at ISTA.","date_published":"2026-01-28T00:00:00Z","OA_type":"hybrid","file":[{"relation":"main_file","creator":"dernst","file_size":2230841,"file_name":"2026_RoyalSocPubProceedingsB_Barata.pdf","success":1,"date_created":"2026-02-16T09:26:02Z","file_id":"21226","date_updated":"2026-02-16T09:26:02Z","content_type":"application/pdf","access_level":"open_access","checksum":"d76afebca0a6f112df0146ae2d929f36"}],"date_created":"2026-02-08T23:02:49Z","external_id":{"pmid":["41592777"]},"issue":"2063","status":"public","language":[{"iso":"eng"}],"quality_controlled":"1","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"Bio"}],"doi":"10.1098/rspb.2025.2471","oa_version":"Published Version","author":[{"first_name":"Carolina","full_name":"De Castro Barbosa Rodrigues Barata, Carolina","id":"20565186-803f-11ed-ab7e-96a4ff7694ef","last_name":"De Castro Barbosa Rodrigues Barata","orcid":"0000-0003-1945-2245"},{"orcid":"0000-0002-4579-8306","last_name":"Vicoso","first_name":"Beatriz","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","full_name":"Vicoso, Beatriz"}],"publication_identifier":{"eissn":["1471-2954"]},"publication":"Proceedings of the Royal Society B Biological Sciences","date_updated":"2026-07-08T09:17:41Z","year":"2026"},{"supplementarymaterial":"yes","month":"06","main_file_link":[{"url":"https://doi.org/10.1038/s41586-026-10679-1","open_access":"1"}],"oa":1,"article_type":"original","ddc":["570"],"citation":{"ista":"Schwarz LA, Dotter C, Isaev S, Lisi M, Malzl D, Büschl C, Ladstätter S, Oliveira B, Barel M, Basilico B, Chintaluri C, Gorkiewicz S, Goudarzi M, Belinova T, Reichl S, Sendžikaitė G, Arcot Jayaram S, Koppensteiner P, Sommer CM, Vogels TP, Menche J, Adameyko I, Kharchenko PV, Bock C, Novarino G. 2026. Cortical development dynamics across autism spectrum disorder mouse models. Nature.","apa":"Schwarz, L. A., Dotter, C., Isaev, S., Lisi, M., Malzl, D., Büschl, C., … Novarino, G. (2026). Cortical development dynamics across autism spectrum disorder mouse models. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-026-10679-1\">https://doi.org/10.1038/s41586-026-10679-1</a>","short":"L.A. Schwarz, C. Dotter, S. Isaev, M. Lisi, D. Malzl, C. Büschl, S. Ladstätter, B. Oliveira, M. Barel, B. Basilico, C. Chintaluri, S. Gorkiewicz, M. Goudarzi, T. Belinova, S. Reichl, G. Sendžikaitė, S. Arcot Jayaram, P. Koppensteiner, C.M. Sommer, T.P. Vogels, J. Menche, I. Adameyko, P.V. Kharchenko, C. Bock, G. Novarino, Nature (2026).","ieee":"L. A. Schwarz <i>et al.</i>, “Cortical development dynamics across autism spectrum disorder mouse models,” <i>Nature</i>. Springer Nature, 2026.","chicago":"Schwarz, Lena A, Christoph Dotter, Sergey Isaev, Michela Lisi, Daniel Malzl, Christoph Büschl, Sabrina Ladstätter, et al. “Cortical Development Dynamics across Autism Spectrum Disorder Mouse Models.” <i>Nature</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41586-026-10679-1\">https://doi.org/10.1038/s41586-026-10679-1</a>.","ama":"Schwarz LA, Dotter C, Isaev S, et al. Cortical development dynamics across autism spectrum disorder mouse models. <i>Nature</i>. 2026. doi:<a href=\"https://doi.org/10.1038/s41586-026-10679-1\">10.1038/s41586-026-10679-1</a>","mla":"Schwarz, Lena A., et al. “Cortical Development Dynamics across Autism Spectrum Disorder Mouse Models.” <i>Nature</i>, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41586-026-10679-1\">10.1038/s41586-026-10679-1</a>."},"abstract":[{"text":"Despite the functional diversity of over 100 causal genes1,2,3, phenotypic convergence across models may reveal common neurobiological processes in autism spectrum disorder (ASD). Here we profiled 251 samples from 11 monogenic mouse models of ASD using single-nucleus multi-omic sequencing across three developmental stages, both sexes and two brain regions. Despite genetic heterogeneity, ASD-linked mutations converged on perturbations of the radial glial cell lineage. These alterations reflect a transient developmental delay rather than lasting lineage misspecification and resolve by postnatal stages. Molecularly, the largest transcriptional differences emerged in neurons at early postnatal stages. These changes included downregulation of synaptic and ion channel-related genes, consistent with homeostatic adaptation or delayed maturation. Network analysis showed molecular convergence across models within each developmental stage, suggesting that diverse mutations linked to ASD impinge on common, stage-specific processes. Convergence becomes less pronounced by postnatal day 14, highlighting the dynamic nature of ASD-associated changes. Cross-genotype heterogeneity is superimposed on stage-specific effects. Electrophysiology corroborated this pattern: mutants generally showed altered neuronal excitability and synaptic properties with model-specific nuances. Our study also highlighted sex-specific gene expression alterations, with female mice often displaying larger effect sizes than male mice. Together, our findings provide a comprehensive view of developmental cellular and molecular dynamics across models of ASD.","lang":"eng"}],"day":"17","pmid":1,"OA_place":"publisher","publication_status":"epub_ahead","department":[{"_id":"AnKi"},{"_id":"GaNo"},{"_id":"TiVo"},{"_id":"ScienComp"},{"_id":"GradSch"},{"_id":"Bio"},{"_id":"PreCl"}],"scopus_import":"1","article_processing_charge":"Yes (via OA deal)","publisher":"Springer Nature","researchdata_availability":"yes","type":"journal_article","acknowledgement":"We thank F. Freeman, V. Voronin and M. Ladron de Guevara for technical assistance; A. Stichelberger and S. Liegenfeld for the management of our animal colony; M. Schunn, C. Gold and the Preclinical Facility team for technical assistance; C. Jansen and the Scientific Computing Facility for bioinformatics support and technical assistance; the Biomedical Sequencing Facility at CeMM for assistance with next-generation sequencing; and J. Lin and T. Krausgruber in the laboratory of C. Bock for support with flow cytometry; J. Kirchner for illustrating the multi-omics approach depicted in Fig. 1; and all members of the laboratory of G.N. for their support and discussions. This study was supported by the Scientific Service Units of ISTA through resources provided by the Imaging & Optics Facility and the Laboratory Support Facility. Bulk RNA-seq was performed by the Next Generation Sequencing Facility at Vienna BioCenter Core Facilities, member of the Vienna BioCenter. This work was supported by a European Research Council Consolidator Grant (PR1028ERC02), by SFARI (PR1028SIM02) and by the Austrian Science Fund (PE1028W1232 and PR1028FG1803) to G.N. Open access funding provided by Institute of Science and Technology (IST Austria).","PlanS_conform":"1","dataavailabilitystatement":"Single-nucleus multiomics data are available from the Gene Expression Omnibus (GSE328363). The mm10 reference genome was used for the alignment (refdata-cellranger-arc-mm10-2020-A-2.0.0, obtained from https://cf.10xgenomics.com/supp/cell-arc/refdata-cellranger-arc-mm10-2020-A-2.0.0.tar.gz). Single-cell data can be accessed and visualized through a CELLxGENE database (https://adameykolab.hifo.meduniwien.ac.at/cellxgene_public/filecrawl/.2026_Nature_Schwarz). Source data are provided with this paper. Scripts and analyses that support the main findings of this study are accessible in a GitHub repository (https://git.ista.ac.at/research-sofware/mouseome).","project":[{"grant_number":"101044865","_id":"34ba8964-11ca-11ed-8bc3-e15864e7e9a6","name":"Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders"},{"name":"Critical windows and reversibility of ASD associated with mutations in chromatin remodelers","grant_number":"707964","_id":"9B91375C-BA93-11EA-9121-9846C619BF3A"},{"call_identifier":"FWF","grant_number":"W1232","_id":"2548AE96-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets"},{"name":"Neurobiology of anxiety in autism spectrum disorders","_id":"ebb38b5d-77a9-11ec-83b8-a42e08120a88","grant_number":"FG1803 49015"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","title":"Cortical development dynamics across autism spectrum disorder mouse models","_id":"22295","corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","date_updated":"2026-07-13T12:58:19Z","publication":"Nature","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"author":[{"full_name":"Schwarz, Lena A","first_name":"Lena A","id":"29A8453C-F248-11E8-B48F-1D18A9856A87","last_name":"Schwarz"},{"orcid":"0000-0002-9033-9096","first_name":"Christoph","last_name":"Dotter","id":"4C66542E-F248-11E8-B48F-1D18A9856A87","full_name":"Dotter, Christoph"},{"full_name":"Isaev, Sergey","first_name":"Sergey","last_name":"Isaev"},{"last_name":"Lisi","first_name":"Michela","id":"39383c1b-d3eb-11ef-8d6c-c8cdf4e10c8c","full_name":"Lisi, Michela"},{"full_name":"Malzl, Daniel","first_name":"Daniel","last_name":"Malzl"},{"first_name":"Christoph","last_name":"Büschl","full_name":"Büschl, Christoph","id":"2a8c054c-0913-11ee-9159-f8ef515809ed"},{"full_name":"Ladstätter, Sabrina","last_name":"Ladstätter","first_name":"Sabrina"},{"full_name":"Oliveira, Bárbara","id":"3B03AA1A-F248-11E8-B48F-1D18A9856A87","first_name":"Bárbara","last_name":"Oliveira"},{"full_name":"Barel, Matteo","last_name":"Barel","id":"8959927b-2236-11ed-bd6e-ea83d94ade0e","first_name":"Matteo"},{"full_name":"Basilico, Bernadette","first_name":"Bernadette","last_name":"Basilico","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","orcid":"0000-0003-1843-3173"},{"last_name":"Chintaluri","full_name":"Chintaluri, Chaitanya","id":"BA06AFEE-A4BA-11EA-AE5C-14673DDC885E","first_name":"Chaitanya","orcid":"0000-0003-4252-1608"},{"last_name":"Gorkiewicz","id":"f141a35d-15a9-11ec-9fb2-fef6becc7b6f","first_name":"Sarah","full_name":"Gorkiewicz, Sarah"},{"id":"3384113A-F248-11E8-B48F-1D18A9856A87","first_name":"Mohammad","full_name":"Goudarzi, Mohammad","last_name":"Goudarzi"},{"id":"0bf89b6a-d28b-11eb-8bd6-f43768e4d368","last_name":"Belinova","first_name":"Tereza","full_name":"Belinova, Tereza"},{"full_name":"Reichl, Stephan","last_name":"Reichl","first_name":"Stephan"},{"id":"dd6d52f2-c50d-11eb-9548-bcf0ff82b344","full_name":"Sendžikaitė, Gintarė","last_name":"Sendžikaitė","first_name":"Gintarė"},{"last_name":"Arcot Jayaram","full_name":"Arcot Jayaram, Satish","id":"b0bbee33-09f7-11eb-909c-8b358058d28a","first_name":"Satish","orcid":"0000-0002-2479-2669"},{"id":"3B8B25A8-F248-11E8-B48F-1D18A9856A87","last_name":"Koppensteiner","first_name":"Peter","full_name":"Koppensteiner, Peter","orcid":"0000-0002-3509-1948"},{"first_name":"Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M","orcid":"0000-0003-1216-9105"},{"last_name":"Vogels","id":"CB6FF8D2-008F-11EA-8E08-2637E6697425","first_name":"Tim P","full_name":"Vogels, Tim P","orcid":"0000-0003-3295-6181"},{"first_name":"Jörg","full_name":"Menche, Jörg","last_name":"Menche"},{"last_name":"Adameyko","full_name":"Adameyko, Igor","first_name":"Igor"},{"first_name":"Peter Vasili","last_name":"Kharchenko","full_name":"Kharchenko, Peter Vasili","id":"0095641e-7eb7-11f1-8665-aec51a2ab5e0"},{"first_name":"Christoph","full_name":"Bock, Christoph","last_name":"Bock"},{"first_name":"Gaia","last_name":"Novarino","full_name":"Novarino, Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178"}],"oa_version":"Published Version","doi":"10.1038/s41586-026-10679-1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"quality_controlled":"1","language":[{"iso":"eng"}],"status":"public","external_id":{"pmid":["42310454"]},"date_created":"2026-07-13T09:47:21Z","OA_type":"hybrid","date_published":"2026-06-17T00:00:00Z"},{"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","title":"Auxin response and PIN‐mediated transport in chlorophyte algae","_id":"22301","corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","acknowledgement":"Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria.","PlanS_conform":"1","project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"external_id":{"pmid":["42271607"]},"date_created":"2026-07-13T10:44:55Z","OA_type":"hybrid","date_published":"2026-06-10T00:00:00Z","date_updated":"2026-07-13T14:26:31Z","year":"2026","publication":"Journal of Integrative Plant Biology","publication_identifier":{"eissn":["1744-7909"],"issn":["1672-9072"]},"author":[{"full_name":"Smoljan, Adrijana","last_name":"Smoljan","first_name":"Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b"},{"full_name":"Koutnik‐Abele, Sarah","last_name":"Koutnik‐Abele","first_name":"Sarah"},{"full_name":"Vladimirtsev, Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","first_name":"Dmitrii","last_name":"Vladimirtsev"},{"last_name":"Klíma","full_name":"Klíma, Petr","first_name":"Petr"},{"full_name":"Bírošíková, Anita","last_name":"Bírošíková","first_name":"Anita"},{"full_name":"Zhang, Yuzhou","last_name":"Zhang","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","first_name":"Yuzhou","orcid":"0000-0003-2627-6956"},{"orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack"},{"full_name":"Schuster, Maximilian","id":"37e65def-d415-11eb-ae59-a7b67be103db","last_name":"Schuster","first_name":"Maximilian"},{"last_name":"Kurtović","first_name":"Katarina","full_name":"Kurtović, Katarina"},{"last_name":"Hammes","first_name":"Ulrich Z.","full_name":"Hammes, Ulrich Z."},{"last_name":"Petrášek","full_name":"Petrášek, Jan","first_name":"Jan"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"doi":"10.1111/jipb.70309","acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"oa_version":"Published Version","quality_controlled":"1","language":[{"iso":"eng"}],"status":"public","article_type":"original","ddc":["580"],"abstract":[{"lang":"eng","text":"Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage."}],"citation":{"ista":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309.","apa":"Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>","short":"A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026).","ama":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. 2026. doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>","chicago":"Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>.","ieee":"A. Smoljan <i>et al.</i>, “Auxin response and PIN‐mediated transport in chlorophyte algae,” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026.","mla":"Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>, jipb. 70309, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>."},"day":"10","pmid":1,"supplementarymaterial":"yes","month":"06","main_file_link":[{"url":"https://doi.org/10.1111/jipb.70309","open_access":"1"}],"oa":1,"publisher":"Wiley","researchdata_availability":"no","das_tickbox":"0","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"NanoFab"},{"_id":"Bio"}],"OA_place":"publisher","publication_status":"epub_ahead","article_number":"jipb.70309","scopus_import":"1","article_processing_charge":"Yes (via OA deal)"},{"type":"preprint","acknowledgement":"We thank all members of the Heisenberg group for discussion and feedback on the manuscript, and the Imaging and Optics Facility, the Life Science Support Facility and the Electron Microscopy Facility of the Institute of Science and Technology Austria (ISTA) for their continued support. We are grateful to M. Sonawane (Tata Institute of Fundamental Research, India) for providing the pCS2-HA-aPKC (PKCι)-V260F (DN) and pCS2-HA-aPKC (PKCι)-A122E (CA) plasmids, and to I. Mayer for the discussion. Molecular graphics and analyses were performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases. This research was funded in whole or in part by the Austrian Science Fund (FWF; grant no. PAT5044023) to C.-P.H., and by a JSPS Overseas Research Fellowship and an EMBO Postdoctoral Fellowship (ALTF 16-2022) to N.H.","dataavailabilitystatement":"The MATLAB code for image analysis, and the full model code, including all parameter values\r\nand condition-specific settings, are available on GitHub at https://github.com/uday2607/EVL-tension-homeostasis.git.","project":[{"name":"Keratins in epithelial tissue spreading","_id":"8f060199-16d5-11f0-9cad-f3253b266c46","grant_number":"PAT 5044023"},{"name":"Mechanosensitive signaling activation in the crosstalk between mechanical force and tissuefluidity","_id":"34dd7f3b-11ca-11ed-8bc3-856f2c87f5da","grant_number":"LTF 16-2022"}],"has_accepted_license":"1","related_material":{"record":[{"status":"public","relation":"earlier_version","id":"21864"}]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Apical domain mechanosensation regulates tissue tension homeostasis","_id":"22276","author":[{"last_name":"Hino","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","full_name":"Hino, Naoya","first_name":"Naoya"},{"first_name":"Tushna","id":"e3b3eda7-fd4d-11eb-8fd8-c40af7a478b1","full_name":"Kapoor, Tushna","last_name":"Kapoor"},{"full_name":"Gubbala, Uday R","id":"bb4a0dc4-32c9-11ee-b5ce-a97ceedd5924","last_name":"Gubbala","first_name":"Uday R"},{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"date_updated":"2026-07-14T07:07:41Z","year":"2026","language":[{"iso":"eng"}],"status":"public","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"oa_version":"Preprint","OA_type":"green","date_published":"2026-07-14T00:00:00Z","date_created":"2026-07-13T09:03:26Z","file":[{"date_created":"2026-07-13T09:16:20Z","success":1,"creator":"nhino","relation":"main_file","file_name":"Main_text_and_figures.pdf","file_size":12477675,"checksum":"66444afd243dce7d383d52d44e8d34a4","date_updated":"2026-07-13T09:16:20Z","content_type":"application/pdf","access_level":"open_access","file_id":"22283"},{"file_name":"Supplementary_figures.pdf","file_size":4545901,"creator":"nhino","relation":"main_file","date_created":"2026-07-13T09:16:25Z","success":1,"access_level":"open_access","content_type":"application/pdf","date_updated":"2026-07-13T09:16:25Z","file_id":"22284","checksum":"90bceb34de64ec792c5de117f0890d05"},{"success":1,"date_created":"2026-07-13T09:16:28Z","file_size":10349451,"file_name":"Supplementary_Video1.mp4","creator":"nhino","relation":"main_file","checksum":"9d9ab89c372142f2ffb6c8c625334d7f","file_id":"22285","content_type":"video/mp4","access_level":"open_access","date_updated":"2026-07-13T09:16:28Z"}],"supplementarymaterial":"yes","month":"07","oa":1,"citation":{"mla":"Hino, Naoya, et al. <i>Apical Domain Mechanosensation Regulates Tissue Tension Homeostasis</i>. Institute of Science and Technology Austria.","ieee":"N. Hino, T. Kapoor, U. R. Gubbala, E. B. Hannezo, and C.-P. J. Heisenberg, “Apical domain mechanosensation regulates tissue tension homeostasis.” Institute of Science and Technology Austria.","ama":"Hino N, Kapoor T, Gubbala UR, Hannezo EB, Heisenberg C-PJ. Apical domain mechanosensation regulates tissue tension homeostasis.","chicago":"Hino, Naoya, Tushna Kapoor, Uday R Gubbala, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Apical Domain Mechanosensation Regulates Tissue Tension Homeostasis.” Institute of Science and Technology Austria, n.d.","short":"N. Hino, T. Kapoor, U.R. Gubbala, E.B. Hannezo, C.-P.J. Heisenberg, (n.d.).","ista":"Hino N, Kapoor T, Gubbala UR, Hannezo EB, Heisenberg C-PJ. Apical domain mechanosensation regulates tissue tension homeostasis.","apa":"Hino, N., Kapoor, T., Gubbala, U. R., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (n.d.). Apical domain mechanosensation regulates tissue tension homeostasis. Institute of Science and Technology Austria."},"abstract":[{"text":"Tissue tension is a key determinant of tissue shape, and its regulation is essential for both morphogenesis and the maintenance of tissue integrity. During zebrafish embryogenesis, the enveloping layer (EVL) – an epithelial monolayer covering the blastoderm – undergoes extensive spreading that is driven by pulling forces exerted at its margin and more than doubles its surface area. Yet whether and how the EVL actively regulates its tissue tension during this process remains unclear. Here, we show that the EVL maintains constant tissue tension while spreading, and that it achieves this by reducing apical cell contractility in response to the same pulling forces that drive its spreading. We identify a mechanosensitive pathway underlying this response, mediated by the scaffold/adaptor protein Kibra regulating the activity of atypical protein kinase C (aPKC) at the apical domain of EVL cells. Under low mechanical stretch, Kibra forms condensates at the base of actin-based apical projections, where it activates Myosin II to increase apical contractility through aPKC downregulation. As mechanical stretch increases, apical projections disassemble, Kibra condensates dissolve, and aPKC activity rises. Elevated aPKC activity in turn reduces apical contractility by reducing Myosin II activity, thereby maintaining constant tissue tension despite increased mechanical stretch. Together, these findings reveal a mechanosensitive mechanism that enables robust adaptation of tissue tension to changing mechanical stretch, ensuring efficient tissue spreading and morphogenesis.","lang":"eng"}],"ddc":["570"],"day":"14","file_date_updated":"2026-07-13T09:16:28Z","publication_status":"draft","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"GradSch"}],"OA_place":"publisher","article_processing_charge":"No","publisher":"Institute of Science and Technology Austria","das_tickbox":"1","keyword":["Epithelial spreading","tissue tension","mechanosensation","aPKC","Kibra","zebrafish"],"researchdata_availability":"yes"},{"department":[{"_id":"MiSi"}],"publication_status":"epub_ahead","OA_place":"publisher","article_processing_charge":"Yes (via OA deal)","scopus_import":"1","publisher":"Springer Nature","DOAJ_listed":"1","das_tickbox":"1","researchdata_availability":"yes","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s44319-026-00861-x"}],"month":"07","supplementarymaterial":"yes","oa":1,"citation":{"chicago":"Dehio, Philippe G, Céline Michard, Juan Carlos Yam-Puc, Adrià Arnau Martí I Líndez, Anett Jandke, Gunhild Unterstab, Lucien Fabre, et al. “A Conserved VPS34-PIKfyve-TRPML1-Myosin II Axis Regulates the Speed of Amoeboid Cell Migration.” <i>EMBO Reports</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s44319-026-00861-x\">https://doi.org/10.1038/s44319-026-00861-x</a>.","ieee":"P. G. Dehio <i>et al.</i>, “A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration,” <i>EMBO Reports</i>. Springer Nature, 2026.","ama":"Dehio PG, Michard C, Yam-Puc JC, et al. A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration. <i>EMBO Reports</i>. 2026. doi:<a href=\"https://doi.org/10.1038/s44319-026-00861-x\">10.1038/s44319-026-00861-x</a>","mla":"Dehio, Philippe G., et al. “A Conserved VPS34-PIKfyve-TRPML1-Myosin II Axis Regulates the Speed of Amoeboid Cell Migration.” <i>EMBO Reports</i>, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s44319-026-00861-x\">10.1038/s44319-026-00861-x</a>.","apa":"Dehio, P. G., Michard, C., Yam-Puc, J. C., Martí I Líndez, A. A., Jandke, A., Unterstab, G., … Hess, C. (2026). A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration. <i>EMBO Reports</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s44319-026-00861-x\">https://doi.org/10.1038/s44319-026-00861-x</a>","ista":"Dehio PG, Michard C, Yam-Puc JC, Martí I Líndez AA, Jandke A, Unterstab G, Fabre L, Sauteur L, Artinger M, Legler DF, Sixt MK, Schaefer T, Wymann MP, Okkenhaug K, Soldati T, Mehling M, Hess C. 2026. A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration. EMBO Reports.","short":"P.G. Dehio, C. Michard, J.C. Yam-Puc, A.A. Martí I Líndez, A. Jandke, G. Unterstab, L. Fabre, L. Sauteur, M. Artinger, D.F. Legler, M.K. Sixt, T. Schaefer, M.P. Wymann, K. Okkenhaug, T. Soldati, M. Mehling, C. Hess, EMBO Reports (2026)."},"abstract":[{"text":"Amoeboid cell migration is key to efficient T cell immunity. Spatial polarization of organelles within cells, including endo-lysosomes, is a prerequisite of migration. However, how ultrastructural polarization is linked to the signaling requirements governing T cell migration remains unknown. Here we show that signaling molecules generated by endo-lysosome-localized kinases regulate velocity of amoeboid migration. Specifically, imaging of T cells identifies accumulation of endo-lysosomes decorated with the lipid kinases VPS34–PIKfyve at the uropod of polarized cells. Activity of VPS34 and PIKfyve regulates speed, but not directedness, of migrating T cells. Mechanistically, PI(3,5)P2 generated by the sequential action of VPS34 and PIKfyve, mediates Ca2+ efflux from lysosomes via the mucolipin TRP cation channel 1 (TRPML1), thus controlling activity of myosin IIA and hence the generation of propulsive force through retrograde actin flow. The VPS34–PIKfyve kinases also regulate velocity of myeloid cells, as well as of the amoeba Dictyostelium discoideum – establishing the axis as an evolutionarily conserved speed control system of amoeboid cell migration.","lang":"eng"}],"article_type":"original","pmid":1,"day":"07","author":[{"full_name":"Dehio, Philippe G","first_name":"Philippe G","id":"b769738e-a003-11ee-b1b8-9030316e0d59","last_name":"Dehio"},{"first_name":"Céline","full_name":"Michard, Céline","last_name":"Michard"},{"last_name":"Yam-Puc","first_name":"Juan Carlos","full_name":"Yam-Puc, Juan Carlos"},{"first_name":"Adrià Arnau","full_name":"Martí I Líndez, Adrià Arnau","last_name":"Martí I Líndez"},{"first_name":"Anett","last_name":"Jandke","full_name":"Jandke, Anett"},{"full_name":"Unterstab, Gunhild","last_name":"Unterstab","first_name":"Gunhild"},{"first_name":"Lucien","last_name":"Fabre","full_name":"Fabre, Lucien"},{"first_name":"Loïc","full_name":"Sauteur, Loïc","last_name":"Sauteur"},{"full_name":"Artinger, Marc","last_name":"Artinger","first_name":"Marc"},{"last_name":"Legler","first_name":"Daniel F.","full_name":"Legler, Daniel F."},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Thorsten","last_name":"Schaefer","full_name":"Schaefer, Thorsten"},{"last_name":"Wymann","first_name":"Matthias P.","full_name":"Wymann, Matthias P."},{"last_name":"Okkenhaug","full_name":"Okkenhaug, Klaus","first_name":"Klaus"},{"full_name":"Soldati, Thierry","first_name":"Thierry","last_name":"Soldati"},{"last_name":"Mehling","id":"3C23B994-F248-11E8-B48F-1D18A9856A87","first_name":"Matthias","full_name":"Mehling, Matthias","orcid":"0000-0001-8599-1226"},{"full_name":"Hess, Christoph","last_name":"Hess","first_name":"Christoph"}],"publication_identifier":{"eissn":["1469-3178"]},"publication":"EMBO Reports","date_updated":"2026-07-20T14:28:59Z","year":"2026","status":"public","language":[{"iso":"eng"}],"quality_controlled":"1","doi":"10.1038/s44319-026-00861-x","oa_version":"Published Version","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"external_id":{"pmid":["42414599"]},"date_published":"2026-07-07T00:00:00Z","OA_type":"gold","date_created":"2026-07-19T22:01:48Z","acknowledgement":"We thank the microscopy core facility of the Department of Biomedicine at the University and University Hospital of Basel for their technical support. This research was technically supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). CH was supported by the Swiss National Science Foundation (SNSF) (310030B_201277; 310030_192677; FZEB-0-180487), the ZBF Program Award 2025 (Hans Zäslin Bustany Foundation), and the Novartis Foundation for Medical-Biological Research (NFMBR) (#23A070). PD was supported by the Swiss Academy for Medical Sciences (SAMW) and SNSF (183980, 225441), the NFMBR (#23A070), AlumniMedizin Basel, and the Freiwillige Akademische Gesellschaft Basel. DFL was supported by the SNSF (220205). Open access funding provided by University of Basel.","type":"journal_article","dataavailabilitystatement":"The analysis workflow to quantify vesicle localization can be accessed on GitHub (https://github.com/loicsauteur/vesicle-analysis, version 0.1.1).\r\n\r\nThe source data of this paper are collected in the following database record: biostudies:S-SCDT-10_1038-S44319-026-00861-x.","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"22371","title":"A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration"},{"supplementarymaterial":"no","month":"06","oa":1,"article_type":"original","ddc":["600"],"citation":{"mla":"Goudarzi, Mohammad, et al. “3D Printing in Core Facilities – Low Pain, High Gain.” <i>Journal of Microscopy</i>, vol. 302, no. 3, Wiley, 2026, pp. 382–95, doi:<a href=\"https://doi.org/10.1111/jmi.70106\">10.1111/jmi.70106</a>.","ama":"Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 3D printing in core facilities – Low pain, high gain. <i>Journal of Microscopy</i>. 2026;302(3):382-395. doi:<a href=\"https://doi.org/10.1111/jmi.70106\">10.1111/jmi.70106</a>","ieee":"M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, and G. Krens, “3D printing in core facilities – Low pain, high gain,” <i>Journal of Microscopy</i>, vol. 302, no. 3. Wiley, pp. 382–395, 2026.","chicago":"Goudarzi, Mohammad, Maximilian Schuster, Arthur Milberger, Manuel Gunkel, Stefan Terjung, and Gabriel Krens. “3D Printing in Core Facilities – Low Pain, High Gain.” <i>Journal of Microscopy</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/jmi.70106\">https://doi.org/10.1111/jmi.70106</a>.","short":"M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, G. Krens, Journal of Microscopy 302 (2026) 382–395.","apa":"Goudarzi, M., Schuster, M., Milberger, A., Gunkel, M., Terjung, S., &#38; Krens, G. (2026). 3D printing in core facilities – Low pain, high gain. <i>Journal of Microscopy</i>. Wiley. <a href=\"https://doi.org/10.1111/jmi.70106\">https://doi.org/10.1111/jmi.70106</a>","ista":"Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 2026. 3D printing in core facilities – Low pain, high gain. Journal of Microscopy. 302(3), 382–395."},"abstract":[{"text":"Three-dimensional (3D) printing has rapidly developed from a niche hobbyist activity into a widely accessible and indispensable technology across multiple scientific disciplines. Within microscopy, optical engineering laboratories and imaging core facilities, 3D printing enables creating customised solutions for sample holders, optical components and everyday laboratory tools that traditionally required specialised machining. By providing rapid prototyping, low-cost production and reproducibility, 3D printing facilitates innovation and efficiency in facility operations. This article provides a perspective on the possibilities, challenges, and practical aspects of implementing 3D printing within microscopy core facilities. Instead of providing technical review about 3D printing, we focus on service organisation, user engagement, resource management and community-driven repositories for design dissemination. Our aim is to share insights with those considering the implementation of 3D printing as a service for developing add-on components to ease the operation of different aspects of the machine-park driven services and those who are managing advanced instrumentation within research groups.","lang":"eng"}],"day":"01","pmid":1,"page":"382-395","OA_place":"publisher","publication_status":"published","department":[{"_id":"Bio"}],"file_date_updated":"2026-07-27T14:01:34Z","scopus_import":"1","article_processing_charge":"Yes (via OA deal)","intvolume":"       302","publisher":"Wiley","researchdata_availability":"no","das_tickbox":"0","type":"journal_article","acknowledgement":"This work was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Imaging & Optics Facility (IOF) and the MiBa Machine Shop. Specifically; Robert Hauschild (IOF), sharing designs, insights and pioneering 3D printing activities at the Imaging and Optics Facility; Bernhard Hochreiter (IOF), for support and testing of anoxic chamber. We also thank Ana Rita Carvalho Faria and Oliver Biehlmaier (Biozentrum University of Basel, Imaging Core Facility) for sharing the design of the adopted power meter.\r\nOpen Access funding provided by Institute of Science and Technology Austria.","volume":302,"PlanS_conform":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","title":"3D printing in core facilities – Low pain, high gain","_id":"21883","corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","date_updated":"2026-07-27T14:02:46Z","publication":"Journal of Microscopy","publication_identifier":{"eissn":["1365-2818"],"issn":["0022-2720"]},"author":[{"first_name":"Mohammad","id":"3384113A-F248-11E8-B48F-1D18A9856A87","last_name":"Goudarzi","full_name":"Goudarzi, Mohammad"},{"last_name":"Schuster","full_name":"Schuster, Maximilian","first_name":"Maximilian","id":"37e65def-d415-11eb-ae59-a7b67be103db"},{"first_name":"Arthur","full_name":"Milberger, Arthur","last_name":"Milberger"},{"first_name":"Manuel","full_name":"Gunkel, Manuel","last_name":"Gunkel"},{"last_name":"Terjung","full_name":"Terjung, Stefan","first_name":"Stefan"},{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","last_name":"Krens","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87"}],"oa_version":"Published Version","doi":"10.1111/jmi.70106","acknowledged_ssus":[{"_id":"Bio"},{"_id":"M-Shop"}],"quality_controlled":"1","language":[{"iso":"eng"}],"status":"public","issue":"3","external_id":{"pmid":["42104760"]},"file":[{"creator":"dernst","relation":"main_file","file_name":"2026_JourMicroscopy_Goudarzi.pdf","file_size":4625767,"date_created":"2026-07-27T14:01:34Z","success":1,"date_updated":"2026-07-27T14:01:34Z","access_level":"open_access","content_type":"application/pdf","file_id":"22593","checksum":"06dfad92b1465ed614a1201b4129960a"}],"date_created":"2026-05-17T22:02:11Z","OA_type":"hybrid","date_published":"2026-06-01T00:00:00Z"},{"das_tickbox":"1","publisher":"Institute of Science and Technology Austria","article_processing_charge":"No","file_date_updated":"2026-03-02T10:59:50Z","publication_status":"published","department":[{"_id":"GradSch"},{"_id":"EvBe"}],"OA_place":"repository","page":"185","day":"26","citation":{"short":"S. Riegler, Root System Plasticity under Nutrient Limitation: Investigating Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea  Species, Institute of Science and Technology Austria, 2026.","apa":"Riegler, S. (2026). <i>Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-21360\">https://doi.org/10.15479/AT-ISTA-21360</a>","ista":"Riegler S. 2026. Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species. Institute of Science and Technology Austria.","mla":"Riegler, Stefan. <i>Root System Plasticity under Nutrient Limitation: Investigating Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea  Species</i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21360\">10.15479/AT-ISTA-21360</a>.","chicago":"Riegler, Stefan. “Root System Plasticity under Nutrient Limitation: Investigating Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea  Species.” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-21360\">https://doi.org/10.15479/AT-ISTA-21360</a>.","ieee":"S. Riegler, “Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species,” Institute of Science and Technology Austria, 2026.","ama":"Riegler S. Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species. 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21360\">10.15479/AT-ISTA-21360</a>"},"doi_confirm":"1","ddc":["570","575","583"],"month":"02","date_published":"2026-02-26T00:00:00Z","file":[{"date_created":"2026-03-02T10:59:50Z","creator":"sriegler","relation":"source_file","file_size":31430022,"file_name":"2026_Riegler_Stefan_Thesis.zip","checksum":"2f1f44e8536c2538f94a440217452c9f","date_updated":"2026-03-02T10:59:50Z","access_level":"closed","content_type":"application/x-zip-compressed","file_id":"21386"},{"checksum":"2e8dc39640bc26ae5684c944c619719b","file_id":"21387","date_updated":"2026-03-02T10:59:49Z","access_level":"closed","content_type":"application/pdf","date_created":"2026-03-02T10:59:49Z","relation":"main_file","embargo_to":"open_access","embargo":"2027-02-27","creator":"sriegler","file_size":11635090,"file_name":"2026_Riegler_Stefan_Thesis.pdf"}],"date_created":"2026-02-27T09:08:14Z","alternative_title":["ISTA Thesis"],"language":[{"iso":"eng"}],"status":"public","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"oa_version":"Published Version","doi":"10.15479/AT-ISTA-21360","publication_identifier":{"issn":["2663-337X"]},"author":[{"orcid":"0000-0003-3413-1343","full_name":"Riegler, Stefan","id":"FF6018E0-D806-11E9-8E43-0B14E6697425","last_name":"Riegler","first_name":"Stefan"}],"date_updated":"2026-07-27T14:30:08Z","year":"2026","supervisor":[{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"}],"corr_author":"1","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","title":"Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species","degree_awarded":"PhD","_id":"21360","has_accepted_license":"1","related_material":{"record":[{"status":"public","relation":"research_data","id":"21363"}]},"tmp":{"short":"CC BY-SA (4.0)","image":"/images/cc_by_sa.png","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode"},"project":[{"name":"Breeding for coffee and cocoa root resilience in low input farming systems based on improved rootstocks","_id":"34afa094-11ca-11ed-8bc3-a375845a59fb","grant_number":"101060393"}],"acknowledgement":"I would like to acknowledge the Austrian Academy of Sciences (ÖAW) and European\r\nResearch Executive Agency (REA) for funding my research (DOC ÖAW Fellowship\r\n26130, Horizon Europe BOLERO Project 101060393). ","type":"dissertation"}]