[{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2022-03-04T08:17:25Z","doi":"10.1111/cga.12039","acknowledgement":"The authors thank all the members of the Division of Morphogenesis, National Institute for Basic Biology, for their contributions to the research, their encouragement, and helpful discussions, particularly Dr M. Suzuki for his critical reading of the manuscript. We also thank the Model Animal Research and Spectrography and Bioimaging Facilities, NIBB Core Research Facilities, for technical support. M.H. was supported by a research fellowship from the Japan Society for the Promotion of Science (JSPS). Our work introduced in this review was supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan, to N.U.","keyword":["Developmental Biology","Embryology","General Medicine","Pediatrics","Perinatology","and Child Health"],"year":"2014","publication_identifier":{"issn":["0914-3505"]},"publication_status":"published","article_type":"original","title":"Molecular and cellular mechanisms of development underlying congenital diseases","type":"journal_article","isi":1,"language":[{"iso":"eng"}],"pmid":1,"oa_version":"None","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/cga.12039"}],"issue":"1","author":[{"first_name":"Masakazu","full_name":"Hashimoto, Masakazu","last_name":"Hashimoto"},{"full_name":"Morita, Hitoshi","last_name":"Morita","first_name":"Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Naoto","last_name":"Ueno","full_name":"Ueno, Naoto"}],"day":"01","publication":"Congenital Anomalies","date_published":"2014-02-01T00:00:00Z","external_id":{"pmid":["24666178"],"isi":["000331769200001"]},"abstract":[{"lang":"eng","text":"In the last several decades, developmental biology has clarified the molecular mechanisms of embryogenesis and organogenesis. In particular, it has demonstrated that the “tool-kit genes” essential for regulating developmental processes are not only highly conserved among species, but are also used as systems at various times and places in an organism to control distinct developmental events. Therefore, mutations in many of these tool-kit genes may cause congenital diseases involving morphological abnormalities. This link between genes and abnormal morphological phenotypes underscores the importance of understanding how cells behave and contribute to morphogenesis as a result of gene function. Recent improvements in live imaging and in quantitative analyses of cellular dynamics will advance our understanding of the cellular pathogenesis of congenital diseases associated with aberrant morphologies. In these studies, it is critical to select an appropriate model organism for the particular phenomenon of interest."}],"oa":1,"date_updated":"2025-09-29T13:21:58Z","status":"public","_id":"10815","scopus_import":"1","department":[{"_id":"CaHe"}],"quality_controlled":"1","volume":54,"citation":{"mla":"Hashimoto, Masakazu, et al. “Molecular and Cellular Mechanisms of Development Underlying Congenital Diseases.” <i>Congenital Anomalies</i>, vol. 54, no. 1, Wiley, 2014, pp. 1–7, doi:<a href=\"https://doi.org/10.1111/cga.12039\">10.1111/cga.12039</a>.","apa":"Hashimoto, M., Morita, H., &#38; Ueno, N. (2014). Molecular and cellular mechanisms of development underlying congenital diseases. <i>Congenital Anomalies</i>. Wiley. <a href=\"https://doi.org/10.1111/cga.12039\">https://doi.org/10.1111/cga.12039</a>","short":"M. Hashimoto, H. Morita, N. Ueno, Congenital Anomalies 54 (2014) 1–7.","ista":"Hashimoto M, Morita H, Ueno N. 2014. Molecular and cellular mechanisms of development underlying congenital diseases. Congenital Anomalies. 54(1), 1–7.","ama":"Hashimoto M, Morita H, Ueno N. Molecular and cellular mechanisms of development underlying congenital diseases. <i>Congenital Anomalies</i>. 2014;54(1):1-7. doi:<a href=\"https://doi.org/10.1111/cga.12039\">10.1111/cga.12039</a>","chicago":"Hashimoto, Masakazu, Hitoshi Morita, and Naoto Ueno. “Molecular and Cellular Mechanisms of Development Underlying Congenital Diseases.” <i>Congenital Anomalies</i>. Wiley, 2014. <a href=\"https://doi.org/10.1111/cga.12039\">https://doi.org/10.1111/cga.12039</a>.","ieee":"M. Hashimoto, H. Morita, and N. Ueno, “Molecular and cellular mechanisms of development underlying congenital diseases,” <i>Congenital Anomalies</i>, vol. 54, no. 1. Wiley, pp. 1–7, 2014."},"page":"1-7","article_processing_charge":"No","month":"02","intvolume":"        54","publisher":"Wiley"},{"publist_id":"4701","date_updated":"2025-09-29T11:18:02Z","status":"public","_id":"2248","scopus_import":"1","department":[{"_id":"CaHe"}],"quality_controlled":"1","volume":322,"citation":{"short":"D. Capek, B. Metscher, G. Müller, Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 322 (2014) 1–12.","ista":"Capek D, Metscher B, Müller G. 2014. Thumbs down: A molecular-morphogenetic approach to avian digit homology. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 322(1), 1–12.","apa":"Capek, D., Metscher, B., &#38; Müller, G. (2014). Thumbs down: A molecular-morphogenetic approach to avian digit homology. <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/jez.b.22545\">https://doi.org/10.1002/jez.b.22545</a>","mla":"Capek, Daniel, et al. “Thumbs down: A Molecular-Morphogenetic Approach to Avian Digit Homology.” <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>, vol. 322, no. 1, Wiley-Blackwell, 2014, pp. 1–12, doi:<a href=\"https://doi.org/10.1002/jez.b.22545\">10.1002/jez.b.22545</a>.","ieee":"D. Capek, B. Metscher, and G. Müller, “Thumbs down: A molecular-morphogenetic approach to avian digit homology,” <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>, vol. 322, no. 1. Wiley-Blackwell, pp. 1–12, 2014.","chicago":"Capek, Daniel, Brian Metscher, and Gerd Müller. “Thumbs down: A Molecular-Morphogenetic Approach to Avian Digit Homology.” <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1002/jez.b.22545\">https://doi.org/10.1002/jez.b.22545</a>.","ama":"Capek D, Metscher B, Müller G. Thumbs down: A molecular-morphogenetic approach to avian digit homology. <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>. 2014;322(1):1-12. doi:<a href=\"https://doi.org/10.1002/jez.b.22545\">10.1002/jez.b.22545</a>"},"page":"1 - 12","article_processing_charge":"No","month":"01","intvolume":"       322","publisher":"Wiley-Blackwell","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:56:33Z","doi":"10.1002/jez.b.22545","year":"2014","publication_identifier":{"issn":["1552-5007"]},"publication_status":"published","type":"journal_article","title":"Thumbs down: A molecular-morphogenetic approach to avian digit homology","isi":1,"language":[{"iso":"eng"}],"oa_version":"None","issue":"1","author":[{"id":"31C42484-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel","last_name":"Capek","full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940"},{"full_name":"Metscher, Brian","last_name":"Metscher","first_name":"Brian"},{"last_name":"Müller","full_name":"Müller, Gerd","first_name":"Gerd"}],"day":"01","publication":"Journal of Experimental Zoology Part B: Molecular and Developmental Evolution","external_id":{"isi":["000335377400001"]},"date_published":"2014-01-01T00:00:00Z","abstract":[{"lang":"eng","text":"Avian forelimb digit homology remains one of the standard themes in comparative biology and EvoDevo research. In order to resolve the apparent contradictions between embryological and paleontological evidence a variety of hypotheses have been presented in recent years. The proposals range from excluding birds from the dinosaur clade, to assignments of homology by different criteria, or even assuming a hexadactyl tetrapod limb ground state. At present two approaches prevail: the frame shift hypothesis and the pyramid reduction hypothesis. While the former postulates a homeotic shift of digit identities, the latter argues for a gradual bilateral reduction of phalanges and digits. Here we present a new model that integrates elements from both hypotheses with the existing experimental and fossil evidence. We start from the main feature common to both earlier concepts, the initiating ontogenetic event: reduction and loss of the anterior-most digit. It is proposed that a concerted mechanism of molecular regulation and developmental mechanics is capable of shifting the boundaries of hoxD expression in embryonic forelimb buds as well as changing the digit phenotypes. Based on a distinction between positional (topological) and compositional (phenotypic) homology criteria, we argue that the identity of the avian digits is II, III, IV, despite a partially altered phenotype. Finally, we introduce an alternative digit reduction scheme that reconciles the current fossil evidence with the presented molecular-morphogenetic model. Our approach identifies specific experiments that allow to test whether gene expression can be shifted and digit phenotypes can be altered by induced digit loss or digit gain."}]},{"author":[{"id":"2E3E0988-F248-11E8-B48F-1D18A9856A87","first_name":"Julien","last_name":"Compagnon","full_name":"Compagnon, Julien"},{"orcid":"0000-0003-2676-3367","full_name":"Barone, Vanessa","last_name":"Barone","first_name":"Vanessa","id":"419EECCC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Srivarsha","last_name":"Rajshekar","full_name":"Rajshekar, Srivarsha"},{"first_name":"Rita","last_name":"Kottmeier","full_name":"Kottmeier, Rita"},{"id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","first_name":"Kornelija","last_name":"Pranjic-Ferscha","full_name":"Pranjic-Ferscha, Kornelija"},{"last_name":"Behrndt","full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"day":"22","pmid":1,"oa_version":"Published Version","issue":"6","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/25535919","open_access":"1"}],"abstract":[{"lang":"eng","text":"Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the embryo along its left-right (LR) axis. Regional differences in cell shape within the lumen-lining KV epithelium are essential for its LR patterning function. However, the processes by which KV cells acquire their characteristic shapes are largely unknown. Here, we show that the notochord induces regional differences in cell shape within KV by triggering extracellular matrix (ECM) accumulation adjacent to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen growth. Our study provides mechanistic insight into the processes by which KV translates global embryonic patterning into regional cell shape differences required for its LR symmetry-breaking function."}],"publication":"Developmental Cell","date_published":"2014-12-22T00:00:00Z","external_id":{"isi":["000346742900012"],"pmid":["25535919"]},"doi":"10.1016/j.devcel.2014.11.003","acknowledgement":"We are grateful to members of the C.-P.H. lab, M. Concha, D. Siekhaus, and J. Vermot for comments on the manuscript and to M. Furutani-Seiki for sharing reagents. This work was supported by the Institute of Science and Technology Austria and an Alexander von Humboldt Foundation fellowship to J.C.","year":"2014","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2018-12-11T11:54:41Z","type":"journal_article","title":"The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ","language":[{"iso":"eng"}],"isi":1,"publication_status":"published","page":"774 - 783","article_processing_charge":"No","quality_controlled":"1","volume":31,"citation":{"apa":"Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K., Behrndt, M., &#38; Heisenberg, C.-P. J. (2014). The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">https://doi.org/10.1016/j.devcel.2014.11.003</a>","mla":"Compagnon, Julien, et al. “The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ.” <i>Developmental Cell</i>, vol. 31, no. 6, Cell Press, 2014, pp. 774–83, doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">10.1016/j.devcel.2014.11.003</a>.","short":"J. Compagnon, V. Barone, S. Rajshekar, R. Kottmeier, K. Pranjic-Ferscha, M. Behrndt, C.-P.J. Heisenberg, Developmental Cell 31 (2014) 774–783.","ista":"Compagnon J, Barone V, Rajshekar S, Kottmeier R, Pranjic-Ferscha K, Behrndt M, Heisenberg C-PJ. 2014. The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. Developmental Cell. 31(6), 774–783.","ama":"Compagnon J, Barone V, Rajshekar S, et al. The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental Cell</i>. 2014;31(6):774-783. doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">10.1016/j.devcel.2014.11.003</a>","chicago":"Compagnon, Julien, Vanessa Barone, Srivarsha Rajshekar, Rita Kottmeier, Kornelija Pranjic-Ferscha, Martin Behrndt, and Carl-Philipp J Heisenberg. “The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ.” <i>Developmental Cell</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">https://doi.org/10.1016/j.devcel.2014.11.003</a>.","ieee":"J. Compagnon <i>et al.</i>, “The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ,” <i>Developmental Cell</i>, vol. 31, no. 6. Cell Press, pp. 774–783, 2014."},"intvolume":"        31","publisher":"Cell Press","month":"12","date_updated":"2026-06-18T18:12:41Z","publist_id":"5182","oa":1,"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"961"}]},"scopus_import":"1","department":[{"_id":"CaHe"}],"ddc":["570"],"status":"public","_id":"1912","corr_author":"1"},{"article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"has_accepted_license":"1","citation":{"ista":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. 2014. Active elastic thin shell theory for cellular deformations. New Journal of Physics. 16, 065005.","short":"H. Berthoumieux, J.-L. Maître, C.-P.J. Heisenberg, E. Paluch, F. Julicher, G. Salbreux, New Journal of Physics 16 (2014).","apa":"Berthoumieux, H., Maître, J.-L., Heisenberg, C.-P. J., Paluch, E., Julicher, F., &#38; Salbreux, G. (2014). Active elastic thin shell theory for cellular deformations. <i>New Journal of Physics</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">https://doi.org/10.1088/1367-2630/16/6/065005</a>","mla":"Berthoumieux, Hélène, et al. “Active Elastic Thin Shell Theory for Cellular Deformations.” <i>New Journal of Physics</i>, vol. 16, 065005, IOP Publishing, 2014, doi:<a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">10.1088/1367-2630/16/6/065005</a>.","ieee":"H. Berthoumieux, J.-L. Maître, C.-P. J. Heisenberg, E. Paluch, F. Julicher, and G. Salbreux, “Active elastic thin shell theory for cellular deformations,” <i>New Journal of Physics</i>, vol. 16. IOP Publishing, 2014.","ama":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. Active elastic thin shell theory for cellular deformations. <i>New Journal of Physics</i>. 2014;16. doi:<a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">10.1088/1367-2630/16/6/065005</a>","chicago":"Berthoumieux, Hélène, Jean-Léon Maître, Carl-Philipp J Heisenberg, Ewa Paluch, Frank Julicher, and Guillaume Salbreux. “Active Elastic Thin Shell Theory for Cellular Deformations.” <i>New Journal of Physics</i>. IOP Publishing, 2014. <a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">https://doi.org/10.1088/1367-2630/16/6/065005</a>."},"pubrep_id":"429","volume":16,"quality_controlled":"1","publisher":"IOP Publishing","intvolume":"        16","month":"06","date_updated":"2025-09-29T12:16:30Z","oa":1,"publist_id":"5171","ddc":["570"],"department":[{"_id":"CaHe"}],"file_date_updated":"2020-07-14T12:45:21Z","scopus_import":"1","_id":"1923","status":"public","day":"01","author":[{"first_name":"Hélène","full_name":"Berthoumieux, Hélène","last_name":"Berthoumieux"},{"full_name":"Maître, Jean-Léon","last_name":"Maître","first_name":"Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3688-1474"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"},{"first_name":"Ewa","last_name":"Paluch","full_name":"Paluch, Ewa"},{"first_name":"Frank","last_name":"Julicher","full_name":"Julicher, Frank"},{"last_name":"Salbreux","full_name":"Salbreux, Guillaume","first_name":"Guillaume"}],"oa_version":"Published Version","abstract":[{"text":"We derive the equations for a thin, axisymmetric elastic shell subjected to an internal active stress giving rise to active tension and moments within the shell. We discuss the stability of a cylindrical elastic shell and its response to a localized change in internal active stress. This description is relevant to describe the cellular actomyosin cortex, a thin shell at the cell surface behaving elastically at a short timescale and subjected to active internal forces arising from myosin molecular motor activity. We show that the recent observations of cell deformation following detachment of adherent cells (Maître J-L et al 2012 Science 338 253-6) are well accounted for by this mechanical description. The actin cortex elastic and bending moduli can be obtained from a quantitative analysis of cell shapes observed in these experiments. Our approach thus provides a non-invasive, imaging-based method for the extraction of cellular physical parameters.","lang":"eng"}],"external_id":{"isi":["000339083700003"]},"file":[{"checksum":"8dbe81ec656bf1264d8889bda9b2b985","access_level":"open_access","relation":"main_file","file_name":"IST-2016-429-v1+1_document.pdf","file_id":"5202","date_updated":"2020-07-14T12:45:21Z","creator":"system","file_size":941387,"date_created":"2018-12-12T10:16:16Z","content_type":"application/pdf"}],"date_published":"2014-06-01T00:00:00Z","publication":"New Journal of Physics","article_number":"065005","year":"2014","doi":"10.1088/1367-2630/16/6/065005","date_created":"2018-12-11T11:54:44Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"isi":1,"title":"Active elastic thin shell theory for cellular deformations","type":"journal_article","publication_status":"published"},{"abstract":[{"text":"In the past decade carbon nanotubes (CNTs) have been widely studied as a potential drug-delivery system, especially with functionality for cellular targeting. Yet, little is known about the actual process of docking to cell receptors and transport dynamics after internalization. Here we performed single-particle studies of folic acid (FA) mediated CNT binding to human carcinoma cells and their transport inside the cytosol. In particular, we employed molecular recognition force spectroscopy, an atomic force microscopy based method, to visualize and quantify docking of FA functionalized CNTs to FA binding receptors in terms of binding probability and binding force. We then traced individual fluorescently labeled, FA functionalized CNTs after specific uptake, and created a dynamic 'roadmap' that clearly showed trajectories of directed diffusion and areas of nanotube confinement in the cytosol. Our results demonstrate the potential of a single-molecule approach for investigation of drug-delivery vehicles and their targeting capacity.","lang":"eng"}],"publication":"Nanotechnology","external_id":{"isi":["000332669300017"]},"file":[{"relation":"main_file","access_level":"open_access","file_name":"2014_Nanotechnology_Lamprecht.pdf","file_id":"7856","checksum":"df4e03d225a19179e7790f6d87a12332","file_size":3804152,"date_created":"2020-05-15T09:21:19Z","content_type":"application/pdf","date_updated":"2020-07-14T12:45:21Z","creator":"dernst"}],"date_published":"2014-03-28T00:00:00Z","author":[{"full_name":"Lamprecht, Constanze","last_name":"Lamprecht","first_name":"Constanze"},{"last_name":"Plochberger","full_name":"Plochberger, Birgit","first_name":"Birgit"},{"orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","first_name":"Verena","last_name":"Ruprecht","full_name":"Ruprecht, Verena"},{"orcid":"0000-0002-2670-2217","last_name":"Wieser","full_name":"Wieser, Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","first_name":"Stefan"},{"first_name":"Christian","full_name":"Rankl, Christian","last_name":"Rankl"},{"last_name":"Heister","full_name":"Heister, Elena","first_name":"Elena"},{"last_name":"Unterauer","full_name":"Unterauer, Barbara","first_name":"Barbara"},{"full_name":"Brameshuber, Mario","last_name":"Brameshuber","first_name":"Mario"},{"first_name":"Jürgen","last_name":"Danzberger","full_name":"Danzberger, Jürgen"},{"first_name":"Petar","last_name":"Lukanov","full_name":"Lukanov, Petar"},{"first_name":"Emmanuel","last_name":"Flahaut","full_name":"Flahaut, Emmanuel"},{"last_name":"Schütz","full_name":"Schütz, Gerhard","first_name":"Gerhard"},{"full_name":"Hinterdorfer, Peter","last_name":"Hinterdorfer","first_name":"Peter"},{"first_name":"Andreas","last_name":"Ebner","full_name":"Ebner, Andreas"}],"day":"28","oa_version":"Submitted Version","issue":"12","type":"journal_article","title":"A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes","isi":1,"language":[{"iso":"eng"}],"publication_status":"published","article_type":"original","acknowledgement":"This work was supported by EC grant Marie Curie RTN-CT-2006-035616, CARBIO 'Carbon nanotubes for biomedical applications' and Austrian FFG grant mnt-era.net 823980, 'IntelliTip'.\r\n","doi":"10.1088/0957-4484/25/12/125704","year":"2014","article_number":"125704","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:54:45Z","intvolume":"        25","publisher":"IOP Publishing","month":"03","article_processing_charge":"No","volume":25,"has_accepted_license":"1","citation":{"ieee":"C. Lamprecht <i>et al.</i>, “A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes,” <i>Nanotechnology</i>, vol. 25, no. 12. IOP Publishing, 2014.","ama":"Lamprecht C, Plochberger B, Ruprecht V, et al. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. 2014;25(12). doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>","chicago":"Lamprecht, Constanze, Birgit Plochberger, Verena Ruprecht, Stefan Wieser, Christian Rankl, Elena Heister, Barbara Unterauer, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>. IOP Publishing, 2014. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>.","ista":"Lamprecht C, Plochberger B, Ruprecht V, Wieser S, Rankl C, Heister E, Unterauer B, Brameshuber M, Danzberger J, Lukanov P, Flahaut E, Schütz G, Hinterdorfer P, Ebner A. 2014. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. Nanotechnology. 25(12), 125704.","short":"C. Lamprecht, B. Plochberger, V. Ruprecht, S. Wieser, C. Rankl, E. Heister, B. Unterauer, M. Brameshuber, J. Danzberger, P. Lukanov, E. Flahaut, G. Schütz, P. Hinterdorfer, A. Ebner, Nanotechnology 25 (2014).","mla":"Lamprecht, Constanze, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>, vol. 25, no. 12, 125704, IOP Publishing, 2014, doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>.","apa":"Lamprecht, C., Plochberger, B., Ruprecht, V., Wieser, S., Rankl, C., Heister, E., … Ebner, A. (2014). A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>"},"scopus_import":"1","ddc":["570"],"department":[{"_id":"CaHe"},{"_id":"MiSi"}],"file_date_updated":"2020-07-14T12:45:21Z","status":"public","_id":"1925","date_updated":"2025-09-29T12:14:47Z","publist_id":"5169","oa":1},{"intvolume":"        82","publisher":"Wiley-Blackwell","month":"05","page":"717 - 726","article_processing_charge":"No","volume":82,"citation":{"short":"M. Chwastyk, A. Galera Prat, M.K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez, M. Cieplak, Proteins: Structure, Function and Bioinformatics 82 (2014) 717–726.","ista":"Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M, Cieplak M. 2014. Theoretical tests of the mechanical protection strategy in protein nanomechanics. Proteins: Structure, Function and Bioinformatics. 82(5), 717–726.","apa":"Chwastyk, M., Galera Prat, A., Sikora, M. K., Gómez Sicilia, À., Carrión Vázquez, M., &#38; Cieplak, M. (2014). Theoretical tests of the mechanical protection strategy in protein nanomechanics. <i>Proteins: Structure, Function and Bioinformatics</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/prot.24436\">https://doi.org/10.1002/prot.24436</a>","mla":"Chwastyk, Mateusz, et al. “Theoretical Tests of the Mechanical Protection Strategy in Protein Nanomechanics.” <i>Proteins: Structure, Function and Bioinformatics</i>, vol. 82, no. 5, Wiley-Blackwell, 2014, pp. 717–26, doi:<a href=\"https://doi.org/10.1002/prot.24436\">10.1002/prot.24436</a>.","ieee":"M. Chwastyk, A. Galera Prat, M. K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez, and M. Cieplak, “Theoretical tests of the mechanical protection strategy in protein nanomechanics,” <i>Proteins: Structure, Function and Bioinformatics</i>, vol. 82, no. 5. Wiley-Blackwell, pp. 717–726, 2014.","chicago":"Chwastyk, Mateusz, Albert Galera Prat, Mateusz K Sikora, Àngel Gómez Sicilia, Mariano Carrión Vázquez, and Marek Cieplak. “Theoretical Tests of the Mechanical Protection Strategy in Protein Nanomechanics.” <i>Proteins: Structure, Function and Bioinformatics</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1002/prot.24436\">https://doi.org/10.1002/prot.24436</a>.","ama":"Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M, Cieplak M. Theoretical tests of the mechanical protection strategy in protein nanomechanics. <i>Proteins: Structure, Function and Bioinformatics</i>. 2014;82(5):717-726. doi:<a href=\"https://doi.org/10.1002/prot.24436\">10.1002/prot.24436</a>"},"scopus_import":"1","department":[{"_id":"CaHe"}],"status":"public","_id":"1891","date_updated":"2025-09-29T13:06:49Z","publist_id":"5204","abstract":[{"lang":"eng","text":"We provide theoretical tests of a novel experimental technique to determine mechanostability of proteins based on stretching a mechanically protected protein by single-molecule force spectroscopy. This technique involves stretching a homogeneous or heterogeneous chain of reference proteins (single-molecule markers) in which one of them acts as host to the guest protein under study. The guest protein is grafted into the host through genetic engineering. It is expected that unraveling of the host precedes the unraveling of the guest removing ambiguities in the reading of the force-extension patterns of the guest protein. We study examples of such systems within a coarse-grained structure-based model. We consider systems with various ratios of mechanostability for the host and guest molecules and compare them to experimental results involving cohesin I as the guest molecule. For a comparison, we also study the force-displacement patterns in proteins that are linked in a serial fashion. We find that the mechanostability of the guest is similar to that of the isolated or serially linked protein. We also demonstrate that the ideal configuration of this strategy would be one in which the host is much more mechanostable than the single-molecule markers. We finally show that it is troublesome to use the highly stable cystine knot proteins as a host to graft a guest in stretching studies because this would involve a cleaving procedure."}],"publication":"Proteins: Structure, Function and Bioinformatics","external_id":{"isi":["000337280400003"]},"date_published":"2014-05-01T00:00:00Z","author":[{"full_name":"Chwastyk, Mateusz","last_name":"Chwastyk","first_name":"Mateusz"},{"last_name":"Galera Prat","full_name":"Galera Prat, Albert","first_name":"Albert"},{"first_name":"Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","full_name":"Sikora, Mateusz K","last_name":"Sikora"},{"first_name":"Àngel","last_name":"Gómez Sicilia","full_name":"Gómez Sicilia, Àngel"},{"first_name":"Mariano","last_name":"Carrión Vázquez","full_name":"Carrión Vázquez, Mariano"},{"first_name":"Marek","full_name":"Cieplak, Marek","last_name":"Cieplak"}],"day":"01","oa_version":"None","issue":"5","title":"Theoretical tests of the mechanical protection strategy in protein nanomechanics","type":"journal_article","language":[{"iso":"eng"}],"isi":1,"publication_status":"published","doi":"10.1002/prot.24436","acknowledgement":"Grant Nr. 2011/01/N/ST3/02475","year":"2014","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:54:34Z"},{"type":"journal_article","title":"Lateral junction dynamics lead the way out","language":[{"iso":"eng"}],"isi":1,"publication_status":"published","doi":"10.1038/ncb2913","year":"2014","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:54:37Z","abstract":[{"lang":"eng","text":"Epithelial cell layers need to be tightly regulated to maintain their integrity and correct function. Cell integration into epithelial sheets is now shown to depend on the N-WASP-regulated stabilization of cortical F-actin, which generates distinct patterns of apical-lateral contractility at E-cadherin-based cell-cell junctions."}],"publication":"Nature Cell Biology","date_published":"2014-01-31T00:00:00Z","external_id":{"isi":["000331161400001"]},"author":[{"last_name":"Behrndt","full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"day":"31","oa_version":"None","issue":"2","scopus_import":"1","department":[{"_id":"CaHe"}],"status":"public","_id":"1900","corr_author":"1","date_updated":"2025-09-29T12:31:29Z","publist_id":"5195","intvolume":"        16","publisher":"Nature Publishing Group","month":"01","page":"127 - 129","article_processing_charge":"No","volume":16,"quality_controlled":"1","citation":{"ieee":"M. Behrndt and C.-P. J. Heisenberg, “Lateral junction dynamics lead the way out,” <i>Nature Cell Biology</i>, vol. 16, no. 2. Nature Publishing Group, pp. 127–129, 2014.","ama":"Behrndt M, Heisenberg C-PJ. Lateral junction dynamics lead the way out. <i>Nature Cell Biology</i>. 2014;16(2):127-129. doi:<a href=\"https://doi.org/10.1038/ncb2913\">10.1038/ncb2913</a>","chicago":"Behrndt, Martin, and Carl-Philipp J Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/ncb2913\">https://doi.org/10.1038/ncb2913</a>.","ista":"Behrndt M, Heisenberg C-PJ. 2014. Lateral junction dynamics lead the way out. Nature Cell Biology. 16(2), 127–129.","short":"M. Behrndt, C.-P.J. Heisenberg, Nature Cell Biology 16 (2014) 127–129.","mla":"Behrndt, Martin, and Carl-Philipp J. Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” <i>Nature Cell Biology</i>, vol. 16, no. 2, Nature Publishing Group, 2014, pp. 127–29, doi:<a href=\"https://doi.org/10.1038/ncb2913\">10.1038/ncb2913</a>.","apa":"Behrndt, M., &#38; Heisenberg, C.-P. J. (2014). Lateral junction dynamics lead the way out. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2913\">https://doi.org/10.1038/ncb2913</a>"}},{"oa_version":"None","day":"01","author":[{"full_name":"Behrndt, Martin","last_name":"Behrndt","first_name":"Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"}],"file":[{"file_size":24842978,"date_created":"2026-03-09T14:53:14Z","content_type":"application/pdf","date_updated":"2026-03-09T14:53:14Z","creator":"cchlebak","relation":"main_file","access_level":"closed","file_name":"2014 Behrnd final.pdf","file_id":"21421","checksum":"67df5501b1b5b313c3bf9a360d713693"}],"date_published":"2014-08-01T00:00:00Z","abstract":[{"text":"A variety of developmental and disease related processes depend on epithelial cell sheet spreading. In order to gain insight into the biophysical mechanism(s) underlying the tissue morphogenesis we studied the spreading of an epithelium during the early development of the zebrafish embryo. In zebrafish epiboly the enveloping cell layer (EVL), a simple squamous epithelium, spreads over the yolk cell to completely engulf it at the end of gastrulation. Previous studies have proposed that an actomyosin ring forming within the yolk syncytial layer (YSL) acts as purse string that through constriction along its circumference pulls on the margin of the EVL. Direct biophysical evidence for this hypothesis has however been missing. The aim of the thesis was to understand how the actomyosin ring may generate pulling forces onto the EVL and what cellular mechanism(s) may facilitate the spreading of the epithelium. Using laser ablation to measure cortical tension within the actomyosin ring we found an anisotropic tension distribution, which was highest along the circumference of the ring. However the low degree of anisotropy was incompatible with the actomyosin ring functioning as a purse string only. Additionally, we observed retrograde cortical flow from vegetal parts of the ring into the EVL margin. Interpreting the experimental data using a theoretical distribution that models  the tissues as active viscous gels led us to proposen that the actomyosin ring has a twofold contribution to EVL epiboly. It not only acts as a purse string through constriction along its circumference, but in addition constriction along the width of the ring generates pulling forces through friction-resisted cortical flow. Moreover, when rendering the purse string mechanism unproductive EVL epiboly proceeded normally indicating that the flow-friction mechanism is sufficient to drive the process. Aiming to understand what cellular mechanism(s) may facilitate the spreading of the epithelium we found that tension-oriented EVL cell divisions limit tissue anisotropy by releasing tension along the division axis and promote epithelial spreading. Notably, EVL cells undergo ectopic cell fusion in conditions in which oriented-cell division is impaired or the epithelium is mechanically challenged. Taken together our study of EVL epiboly suggests a novel mechanism of force generation for actomyosin rings through friction-resisted cortical flow and highlights the importance of tension-oriented cell divisions in epithelial morphogenesis.","lang":"eng"}],"alternative_title":["IST Austria Thesis"],"date_created":"2018-12-11T11:51:49Z","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","year":"2014","publication_status":"published","language":[{"iso":"eng"}],"type":"dissertation","title":"Forces driving epithelial spreading in zebrafish epiboly","citation":{"apa":"Behrndt, M. (2014). <i>Forces driving epithelial spreading in zebrafish epiboly</i>. IST Austria.","mla":"Behrndt, Martin. <i>Forces Driving Epithelial Spreading in Zebrafish Epiboly</i>. IST Austria, 2014.","ista":"Behrndt M. 2014. Forces driving epithelial spreading in zebrafish epiboly. IST Austria.","short":"M. Behrndt, Forces Driving Epithelial Spreading in Zebrafish Epiboly, IST Austria, 2014.","ama":"Behrndt M. Forces driving epithelial spreading in zebrafish epiboly. 2014.","chicago":"Behrndt, Martin. “Forces Driving Epithelial Spreading in Zebrafish Epiboly.” IST Austria, 2014.","ieee":"M. Behrndt, “Forces driving epithelial spreading in zebrafish epiboly,” IST Austria, 2014."},"has_accepted_license":"1","article_processing_charge":"No","acknowledged_ssus":[{"_id":"SSU"}],"page":"91","month":"08","OA_place":"repository","supervisor":[{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"publisher":"IST Austria","related_material":{"record":[{"id":"2282","status":"public","relation":"part_of_dissertation"},{"id":"2950","relation":"part_of_dissertation","status":"public"},{"id":"3373","relation":"part_of_dissertation","status":"public"}]},"publist_id":"5804","date_updated":"2026-06-18T18:45:01Z","corr_author":"1","_id":"1403","status":"public","file_date_updated":"2026-03-09T14:53:14Z","department":[{"_id":"CaHe"}],"ddc":["590"],"degree_awarded":"PhD"},{"volume":153,"quality_controlled":"1","citation":{"chicago":"Heisenberg, Carl-Philipp J, and Yohanns Bellaïche. “Forces in Tissue Morphogenesis and Patterning.” <i>Cell</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">https://doi.org/10.1016/j.cell.2013.05.008</a>.","ama":"Heisenberg C-PJ, Bellaïche Y. Forces in tissue morphogenesis and patterning. <i>Cell</i>. 2013;153(5):948-962. doi:<a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">10.1016/j.cell.2013.05.008</a>","ieee":"C.-P. J. Heisenberg and Y. Bellaïche, “Forces in tissue morphogenesis and patterning,” <i>Cell</i>, vol. 153, no. 5. Cell Press, pp. 948–962, 2013.","mla":"Heisenberg, Carl-Philipp J., and Yohanns Bellaïche. “Forces in Tissue Morphogenesis and Patterning.” <i>Cell</i>, vol. 153, no. 5, Cell Press, 2013, pp. 948–62, doi:<a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">10.1016/j.cell.2013.05.008</a>.","apa":"Heisenberg, C.-P. J., &#38; Bellaïche, Y. (2013). Forces in tissue morphogenesis and patterning. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">https://doi.org/10.1016/j.cell.2013.05.008</a>","short":"C.-P.J. Heisenberg, Y. Bellaïche, Cell 153 (2013) 948–962.","ista":"Heisenberg C-PJ, Bellaïche Y. 2013. Forces in tissue morphogenesis and patterning. Cell. 153(5), 948–962."},"page":"948 - 962","article_processing_charge":"No","month":"05","intvolume":"       153","publisher":"Cell Press","publist_id":"3966","date_updated":"2025-09-29T13:49:30Z","status":"public","_id":"2833","corr_author":"1","scopus_import":"1","department":[{"_id":"CaHe"}],"oa_version":"None","issue":"5","author":[{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"},{"first_name":"Yohanns","last_name":"Bellaïche","full_name":"Bellaïche, Yohanns"}],"day":"23","publication":"Cell","external_id":{"isi":["000319456800005"]},"date_published":"2013-05-23T00:00:00Z","abstract":[{"text":"During development, mechanical forces cause changes in size, shape, number, position, and gene expression of cells. They are therefore integral to any morphogenetic processes. Force generation by actin-myosin networks and force transmission through adhesive complexes are two self-organizing phenomena driving tissue morphogenesis. Coordination and integration of forces by long-range force transmission and mechanosensing of cells within tissues produce large-scale tissue shape changes. Extrinsic mechanical forces also control tissue patterning by modulating cell fate specification and differentiation. Thus, the interplay between tissue mechanics and biochemical signaling orchestrates tissue morphogenesis and patterning in development.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:59:50Z","acknowledgement":"C.-P.H. is supported by the Institute of Science and Technology Austria and grants from the Deutsche Forschungsgemeinschaft (DFG) and Fonds zur Förderung der wissenschaftlichen Forschung (FWF).","doi":"10.1016/j.cell.2013.05.008","year":"2013","publication_status":"published","title":"Forces in tissue morphogenesis and patterning","type":"journal_article","isi":1,"language":[{"iso":"eng"}]},{"date_created":"2018-12-11T11:59:52Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2013","doi":"10.1016/j.devcel.2013.03.007","publication_status":"published","isi":1,"language":[{"iso":"eng"}],"type":"journal_article","title":"Holding on and letting go: Cadherin turnover in cell intercalation","issue":"6","oa_version":"None","day":"25","author":[{"last_name":"Morita","full_name":"Morita, Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","first_name":"Hitoshi"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"external_id":{"isi":["000317634500003"]},"date_published":"2013-05-25T00:00:00Z","publication":"Developmental Cell","abstract":[{"lang":"eng","text":"In zebrafish early development, blastoderm cells undergo extensive radial intercalations, triggering the spreading of the blastoderm over the yolk cell and thereby initiating embryonic body axis formation. Now reporting in Developmental Cell, Song et al. (2013) demonstrate a critical function for EGF-dependent E-cadherin endocytosis in promoting blastoderm cell intercalations."}],"publist_id":"3956","date_updated":"2025-09-29T13:44:50Z","corr_author":"1","_id":"2841","status":"public","department":[{"_id":"CaHe"}],"scopus_import":"1","citation":{"ieee":"H. Morita and C.-P. J. Heisenberg, “Holding on and letting go: Cadherin turnover in cell intercalation,” <i>Developmental Cell</i>, vol. 24, no. 6. Cell Press, pp. 567–569, 2013.","chicago":"Morita, Hitoshi, and Carl-Philipp J Heisenberg. “Holding on and Letting Go: Cadherin Turnover in Cell Intercalation.” <i>Developmental Cell</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">https://doi.org/10.1016/j.devcel.2013.03.007</a>.","ama":"Morita H, Heisenberg C-PJ. Holding on and letting go: Cadherin turnover in cell intercalation. <i>Developmental Cell</i>. 2013;24(6):567-569. doi:<a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">10.1016/j.devcel.2013.03.007</a>","short":"H. Morita, C.-P.J. Heisenberg, Developmental Cell 24 (2013) 567–569.","ista":"Morita H, Heisenberg C-PJ. 2013. Holding on and letting go: Cadherin turnover in cell intercalation. Developmental Cell. 24(6), 567–569.","apa":"Morita, H., &#38; Heisenberg, C.-P. J. (2013). Holding on and letting go: Cadherin turnover in cell intercalation. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">https://doi.org/10.1016/j.devcel.2013.03.007</a>","mla":"Morita, Hitoshi, and Carl-Philipp J. Heisenberg. “Holding on and Letting Go: Cadherin Turnover in Cell Intercalation.” <i>Developmental Cell</i>, vol. 24, no. 6, Cell Press, 2013, pp. 567–69, doi:<a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">10.1016/j.devcel.2013.03.007</a>."},"volume":24,"quality_controlled":"1","article_processing_charge":"No","page":"567 - 569","month":"05","publisher":"Cell Press","intvolume":"        24"},{"pmid":1,"oa_version":"Submitted Version","issue":"7","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596994/","open_access":"1"}],"author":[{"full_name":"Tay, Hwee","last_name":"Tay","first_name":"Hwee"},{"first_name":"Sabrina","full_name":"Schulze, Sabrina","last_name":"Schulze"},{"last_name":"Compagnon","full_name":"Compagnon, Julien","id":"2E3E0988-F248-11E8-B48F-1D18A9856A87","first_name":"Julien"},{"first_name":"Fiona","last_name":"Foley","full_name":"Foley, Fiona"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"},{"full_name":"Yost, H Joseph","last_name":"Yost","first_name":"H Joseph"},{"last_name":"Abdelilah Seyfried","full_name":"Abdelilah Seyfried, Salim","first_name":"Salim"},{"last_name":"Amack","full_name":"Amack, Jeffrey","first_name":"Jeffrey"}],"day":"01","publication":"Development","date_published":"2013-04-01T00:00:00Z","external_id":{"isi":["000316096400018"],"pmid":["23482490"]},"abstract":[{"lang":"eng","text":"Motile cilia perform crucial functions during embryonic development and throughout adult life. Development of organs containing motile cilia involves regulation of cilia formation (ciliogenesis) and formation of a luminal space (lumenogenesis) in which cilia generate fluid flows. Control of ciliogenesis and lumenogenesis is not yet fully understood, and it remains unclear whether these processes are coupled. In the zebrafish embryo, lethal giant larvae 2 (lgl2) is expressed prominently in ciliated organs. Lgl proteins are involved in establishing cell polarity and have been implicated in vesicle trafficking. Here, we identified a role for Lgl2 in development of ciliated epithelia in Kupffer's vesicle, which directs left-right asymmetry of the embryo; the otic vesicles, which give rise to the inner ear; and the pronephric ducts of the kidney. Using Kupffer's vesicle as a model ciliated organ, we found that depletion of Lgl2 disrupted lumen formation and reduced cilia number and length. Immunofluorescence and time-lapse imaging of Kupffer's vesicle morphogenesis in Lgl2-deficient embryos suggested cell adhesion defects and revealed loss of the adherens junction component E-cadherin at lateral membranes. Genetic interaction experiments indicate that Lgl2 interacts with Rab11a to regulate E-cadherin and mediate lumen formation that is uncoupled from cilia formation. These results uncover new roles and interactions for Lgl2 that are crucial for both lumenogenesis and ciliogenesis and indicate that these processes are genetically separable in zebrafish."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:59:59Z","doi":"10.1242/dev.087130","acknowledgement":"Deposited in PMC for release after 12 months. We thank members of the Amack lab for helpful discussions and Mahendra Sonawane for donating reagents.","year":"2013","publication_status":"published","type":"journal_article","title":"Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle","language":[{"iso":"eng"}],"isi":1,"volume":140,"quality_controlled":"1","citation":{"ieee":"H. Tay <i>et al.</i>, “Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle,” <i>Development</i>, vol. 140, no. 7. Company of Biologists, pp. 1550–1559, 2013.","ama":"Tay H, Schulze S, Compagnon J, et al. Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle. <i>Development</i>. 2013;140(7):1550-1559. doi:<a href=\"https://doi.org/10.1242/dev.087130\">10.1242/dev.087130</a>","chicago":"Tay, Hwee, Sabrina Schulze, Julien Compagnon, Fiona Foley, Carl-Philipp J Heisenberg, H Joseph Yost, Salim Abdelilah Seyfried, and Jeffrey Amack. “Lethal Giant Larvae 2 Regulates Development of the Ciliated Organ Kupffer’s Vesicle.” <i>Development</i>. Company of Biologists, 2013. <a href=\"https://doi.org/10.1242/dev.087130\">https://doi.org/10.1242/dev.087130</a>.","ista":"Tay H, Schulze S, Compagnon J, Foley F, Heisenberg C-PJ, Yost HJ, Abdelilah Seyfried S, Amack J. 2013. Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle. Development. 140(7), 1550–1559.","short":"H. Tay, S. Schulze, J. Compagnon, F. Foley, C.-P.J. Heisenberg, H.J. Yost, S. Abdelilah Seyfried, J. Amack, Development 140 (2013) 1550–1559.","apa":"Tay, H., Schulze, S., Compagnon, J., Foley, F., Heisenberg, C.-P. J., Yost, H. J., … Amack, J. (2013). Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.087130\">https://doi.org/10.1242/dev.087130</a>","mla":"Tay, Hwee, et al. “Lethal Giant Larvae 2 Regulates Development of the Ciliated Organ Kupffer’s Vesicle.” <i>Development</i>, vol. 140, no. 7, Company of Biologists, 2013, pp. 1550–59, doi:<a href=\"https://doi.org/10.1242/dev.087130\">10.1242/dev.087130</a>."},"page":"1550 - 1559","article_processing_charge":"No","month":"04","intvolume":"       140","publisher":"Company of Biologists","publist_id":"3927","oa":1,"date_updated":"2025-09-29T13:36:20Z","status":"public","_id":"2862","scopus_import":"1","department":[{"_id":"CaHe"}]},{"oa_version":"None","issue":"2","author":[{"orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","first_name":"Jean-Léon","last_name":"Maître","full_name":"Maître, Jean-Léon"},{"last_name":"Berthoumieux","full_name":"Berthoumieux, Hélène","first_name":"Hélène"},{"last_name":"Krens","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","first_name":"Gabriel","orcid":"0000-0003-4761-5996"},{"first_name":"Guillaume","full_name":"Salbreux, Guillaume","last_name":"Salbreux"},{"first_name":"Frank","full_name":"Julicher, Frank","last_name":"Julicher"},{"full_name":"Paluch, Ewa","last_name":"Paluch","first_name":"Ewa"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}],"day":"01","publication":"Medecine Sciences","date_published":"2013-02-01T00:00:00Z","external_id":{"isi":["000315749700011"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T12:00:08Z","doi":"10.1051/medsci/2013292011","year":"2013","publication_status":"published","project":[{"_id":"252064B8-B435-11E9-9278-68D0E5697425","grant_number":"HE_3231/6-1","name":"Analysis of the Formation and Function of Different Cell Protusion Types During Cell Migration in Vivo"},{"grant_number":"I812-B12","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation","_id":"2527D5CC-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"title":"Cell adhesion mechanics of zebrafish gastrulation","type":"journal_article","isi":1,"language":[{"iso":"eng"}],"volume":29,"quality_controlled":"1","citation":{"ieee":"J.-L. Maître <i>et al.</i>, “Cell adhesion mechanics of zebrafish gastrulation,” <i>Medecine Sciences</i>, vol. 29, no. 2. Éditions Médicales et Scientifiques, pp. 147–150, 2013.","ama":"Maître J-L, Berthoumieux H, Krens G, et al. Cell adhesion mechanics of zebrafish gastrulation. <i>Medecine Sciences</i>. 2013;29(2):147-150. doi:<a href=\"https://doi.org/10.1051/medsci/2013292011\">10.1051/medsci/2013292011</a>","chicago":"Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux, Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Cell Adhesion Mechanics of Zebrafish Gastrulation.” <i>Medecine Sciences</i>. Éditions Médicales et Scientifiques, 2013. <a href=\"https://doi.org/10.1051/medsci/2013292011\">https://doi.org/10.1051/medsci/2013292011</a>.","ista":"Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg C-PJ. 2013. Cell adhesion mechanics of zebrafish gastrulation. Medecine Sciences. 29(2), 147–150.","short":"J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch, C.-P.J. Heisenberg, Medecine Sciences 29 (2013) 147–150.","mla":"Maître, Jean-Léon, et al. “Cell Adhesion Mechanics of Zebrafish Gastrulation.” <i>Medecine Sciences</i>, vol. 29, no. 2, Éditions Médicales et Scientifiques, 2013, pp. 147–50, doi:<a href=\"https://doi.org/10.1051/medsci/2013292011\">10.1051/medsci/2013292011</a>.","apa":"Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch, E., &#38; Heisenberg, C.-P. J. (2013). Cell adhesion mechanics of zebrafish gastrulation. <i>Medecine Sciences</i>. Éditions Médicales et Scientifiques. <a href=\"https://doi.org/10.1051/medsci/2013292011\">https://doi.org/10.1051/medsci/2013292011</a>"},"page":"147 - 150","article_processing_charge":"No","month":"02","intvolume":"        29","publisher":"Éditions Médicales et Scientifiques","publist_id":"3877","date_updated":"2025-09-29T13:33:31Z","status":"public","_id":"2884","scopus_import":"1","department":[{"_id":"CaHe"}]},{"publication_status":"published","language":[{"iso":"eng"}],"isi":1,"type":"journal_article","title":"Anthrax toxin receptor 2a controls mitotic spindle positioning","date_created":"2018-12-11T12:00:20Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2013","acknowledgement":"This work was supported by the SNSF, the Swiss SystemsX.ch initiative and LipidX-2008/011 (M.G-G. and F.G.v.d.G.), by the Fondation SANTE-Vaduz/Aide au Soutien des Nouvelles Thérapies (F.G.v.d.G.) and by the ERC, the NCCR Frontiers in Genetics and Chemical Biology programmes and the Polish–Swiss research program (M.G-G.).","doi":"10.1038/ncb2632","date_published":"2013-01-01T00:00:00Z","external_id":{"isi":["000312841300007"]},"publication":"Nature Cell Biology","abstract":[{"text":"Oriented mitosis is essential during tissue morphogenesis. The Wnt/planar cell polarity (Wnt/PCP) pathway orients mitosis in a number of developmental systems, including dorsal epiblast cell divisions along the animal-vegetal (A-V) axis during zebrafish gastrulation. How Wnt signalling orients the mitotic plane is, however, unknown. Here we show that, in dorsal epiblast cells, anthrax toxin receptor 2a (Antxr2a) accumulates in a polarized cortical cap, which is aligned with the embryonic A-V axis and forecasts the division plane. Filamentous actin (F-actin) also forms an A-V polarized cap, which depends on Wnt/PCP and its effectors RhoA and Rock2. Antxr2a is recruited to the cap by interacting with actin. Antxr2a also interacts with RhoA and together they activate the diaphanous-related formin zDia2. Mechanistically, Antxr2a functions as a Wnt-dependent polarized determinant, which, through the action of RhoA and zDia2, exerts torque on the spindle to align it with the A-V axis.\r\n","lang":"eng"}],"issue":"1","oa_version":"None","day":"01","author":[{"full_name":"Castanon, Irinka","last_name":"Castanon","first_name":"Irinka"},{"first_name":"Laurence","last_name":"Abrami","full_name":"Abrami, Laurence"},{"last_name":"Holtzer","full_name":"Holtzer, Laurent","first_name":"Laurent"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"},{"last_name":"Van Der Goot","full_name":"Van Der Goot, Françoise","first_name":"Françoise"},{"last_name":"González Gaitán","full_name":"González Gaitán, Marcos","first_name":"Marcos"}],"_id":"2918","status":"public","department":[{"_id":"CaHe"}],"scopus_import":"1","publist_id":"3819","date_updated":"2025-09-30T08:16:16Z","month":"01","publisher":"Nature Publishing Group","intvolume":"        15","citation":{"mla":"Castanon, Irinka, et al. “Anthrax Toxin Receptor 2a Controls Mitotic Spindle Positioning.” <i>Nature Cell Biology</i>, vol. 15, no. 1, Nature Publishing Group, 2013, pp. 28–39, doi:<a href=\"https://doi.org/10.1038/ncb2632\">10.1038/ncb2632</a>.","apa":"Castanon, I., Abrami, L., Holtzer, L., Heisenberg, C.-P. J., Van Der Goot, F., &#38; González Gaitán, M. (2013). Anthrax toxin receptor 2a controls mitotic spindle positioning. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2632\">https://doi.org/10.1038/ncb2632</a>","ista":"Castanon I, Abrami L, Holtzer L, Heisenberg C-PJ, Van Der Goot F, González Gaitán M. 2013. Anthrax toxin receptor 2a controls mitotic spindle positioning. Nature Cell Biology. 15(1), 28–39.","short":"I. Castanon, L. Abrami, L. Holtzer, C.-P.J. Heisenberg, F. Van Der Goot, M. González Gaitán, Nature Cell Biology 15 (2013) 28–39.","chicago":"Castanon, Irinka, Laurence Abrami, Laurent Holtzer, Carl-Philipp J Heisenberg, Françoise Van Der Goot, and Marcos González Gaitán. “Anthrax Toxin Receptor 2a Controls Mitotic Spindle Positioning.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2013. <a href=\"https://doi.org/10.1038/ncb2632\">https://doi.org/10.1038/ncb2632</a>.","ama":"Castanon I, Abrami L, Holtzer L, Heisenberg C-PJ, Van Der Goot F, González Gaitán M. Anthrax toxin receptor 2a controls mitotic spindle positioning. <i>Nature Cell Biology</i>. 2013;15(1):28-39. doi:<a href=\"https://doi.org/10.1038/ncb2632\">10.1038/ncb2632</a>","ieee":"I. Castanon, L. Abrami, L. Holtzer, C.-P. J. Heisenberg, F. Van Der Goot, and M. González Gaitán, “Anthrax toxin receptor 2a controls mitotic spindle positioning,” <i>Nature Cell Biology</i>, vol. 15, no. 1. Nature Publishing Group, pp. 28–39, 2013."},"volume":15,"quality_controlled":"1","article_processing_charge":"No","page":"28 - 39"},{"page":"1 - 3","article_processing_charge":"No","volume":32,"quality_controlled":"1","citation":{"mla":"Compagnon, Julien, and Carl-Philipp J. Heisenberg. “Neurulation Coordinating Cell Polarisation and Lumen Formation.” <i>EMBO Journal</i>, vol. 32, no. 1, Wiley-Blackwell, 2013, pp. 1–3, doi:<a href=\"https://doi.org/10.1038/emboj.2012.325\">10.1038/emboj.2012.325</a>.","apa":"Compagnon, J., &#38; Heisenberg, C.-P. J. (2013). Neurulation coordinating cell polarisation and lumen formation. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2012.325\">https://doi.org/10.1038/emboj.2012.325</a>","ista":"Compagnon J, Heisenberg C-PJ. 2013. Neurulation coordinating cell polarisation and lumen formation. EMBO Journal. 32(1), 1–3.","short":"J. Compagnon, C.-P.J. Heisenberg, EMBO Journal 32 (2013) 1–3.","ama":"Compagnon J, Heisenberg C-PJ. Neurulation coordinating cell polarisation and lumen formation. <i>EMBO Journal</i>. 2013;32(1):1-3. doi:<a href=\"https://doi.org/10.1038/emboj.2012.325\">10.1038/emboj.2012.325</a>","chicago":"Compagnon, Julien, and Carl-Philipp J Heisenberg. “Neurulation Coordinating Cell Polarisation and Lumen Formation.” <i>EMBO Journal</i>. Wiley-Blackwell, 2013. <a href=\"https://doi.org/10.1038/emboj.2012.325\">https://doi.org/10.1038/emboj.2012.325</a>.","ieee":"J. Compagnon and C.-P. J. Heisenberg, “Neurulation coordinating cell polarisation and lumen formation,” <i>EMBO Journal</i>, vol. 32, no. 1. Wiley-Blackwell, pp. 1–3, 2013."},"intvolume":"        32","publisher":"Wiley-Blackwell","month":"01","date_updated":"2025-09-29T13:27:27Z","publist_id":"3817","oa":1,"scopus_import":"1","department":[{"_id":"CaHe"}],"status":"public","corr_author":"1","_id":"2920","author":[{"first_name":"Julien","id":"2E3E0988-F248-11E8-B48F-1D18A9856A87","full_name":"Compagnon, Julien","last_name":"Compagnon"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"day":"09","oa_version":"Submitted Version","pmid":1,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545307/"}],"issue":"1","abstract":[{"lang":"eng","text":"Cell polarisation in development is a common and fundamental process underlying embryo patterning and morphogenesis, and has been extensively studied over the past years. Our current knowledge of cell polarisation in development is predominantly based on studies that have analysed polarisation of single cells, such as eggs, or cellular aggregates with a stable polarising interface, such as cultured epithelial cells (St Johnston and Ahringer, 2010). However, in embryonic development, particularly of vertebrates, cell polarisation processes often encompass large numbers of cells that are placed within moving and proliferating tissues, and undergo mesenchymal-to-epithelial transitions with a highly complex spatiotemporal choreography. How such intricate cell polarisation processes in embryonic development are achieved has only started to be analysed. By using live imaging of neurulation in the transparent zebrafish embryo, Buckley et al (2012) now describe a novel polarisation strategy by which cells assemble an apical domain in the part of their cell body that intersects with the midline of the forming neural rod. This mechanism, along with the previously described mirror-symmetric divisions (Tawk et al, 2007), is thought to trigger formation of both neural rod midline and lumen."}],"publication":"EMBO Journal","date_published":"2013-01-09T00:00:00Z","external_id":{"isi":["000314141900001"],"pmid":["23211745"]},"doi":"10.1038/emboj.2012.325","year":"2013","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T12:00:20Z","title":"Neurulation coordinating cell polarisation and lumen formation","type":"journal_article","isi":1,"language":[{"iso":"eng"}],"publication_status":"published"},{"publication":"Current Biology","external_id":{"isi":["000313383700026"]},"date_published":"2013-01-07T00:00:00Z","abstract":[{"text":"To fight infectious diseases, host immune defenses are employed at multiple levels. Sanitary behavior, such as pathogen avoidance and removal, acts as a first line of defense to prevent infection [1] before activation of the physiological immune system. Insect societies have evolved a wide range of collective hygiene measures and intensive health care toward pathogen-exposed group members [2]. One of the most common behaviors is allogrooming, in which nestmates remove infectious particles from the body surfaces of exposed individuals [3]. Here we show that, in invasive garden ants, grooming of fungus-exposed brood is effective beyond the sheer mechanical removal of fungal conidiospores; it also includes chemical disinfection through the application of poison produced by the ants themselves. Formic acid is the main active component of the poison. It inhibits fungal growth of conidiospores remaining on the brood surface after grooming and also those collected in the mouth of the grooming ant. This dual function is achieved by uptake of the poison droplet into the mouth through acidopore self-grooming and subsequent application onto the infectious brood via brood grooming. This extraordinary behavior extends the current understanding of grooming and the establishment of social immunity in insect societies.","lang":"eng"}],"oa_version":"None","issue":"1","author":[{"full_name":"Tragust, Simon","last_name":"Tragust","first_name":"Simon","id":"35A7A418-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Barbara","id":"479DDAAC-E9CD-11E9-9B5F-82450873F7A1","full_name":"Mitteregger, Barbara","last_name":"Mitteregger"},{"full_name":"Barone, Vanessa","last_name":"Barone","first_name":"Vanessa","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367"},{"full_name":"Konrad, Matthias","last_name":"Konrad","first_name":"Matthias","id":"46528076-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ugelvig, Line V","last_name":"Ugelvig","first_name":"Line V","id":"3DC97C8E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1832-8883"},{"full_name":"Cremer, Sylvia","last_name":"Cremer","first_name":"Sylvia","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2193-3868"}],"day":"07","publication_status":"published","title":"Ants disinfect fungus-exposed brood by oral uptake and spread of their poison","type":"journal_article","project":[{"_id":"25DAF0B2-B435-11E9-9278-68D0E5697425","grant_number":"CR-118/3-1","name":"Host-Parasite Coevolution"},{"_id":"25DC711C-B435-11E9-9278-68D0E5697425","name":"Social Vaccination in Ant Colonies: from Individual Mechanisms to Society Effects","grant_number":"243071","call_identifier":"FP7"},{"grant_number":"302004","name":"Collective disease defence and pathogen detection abilities in ant societies: a chemo-neuro-immunological approach","_id":"25DDF0F0-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"language":[{"iso":"eng"}],"isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T12:00:23Z","acknowledgement":"Funding for this project was obtained by the German Research Foundation (DFG, to S.C.) and the European Research Council (ERC, through an ERC-Starting Grant to S.C. and an Individual Marie Curie IEF fellowship to L.V.U.).\r\nWe thank Jørgen Eilenberg, Bernhardt Steinwender, Miriam Stock, and Meghan L. Vyleta for the fungal strain and its characterization; Volker Witte for chemical information; Eva Sixt for ant drawings; and Robert Hauschild for help with image analysis. We further thank Martin Kaltenpoth, Michael Sixt, Jürgen Heinze, and Joachim Ruther for discussion and Daria Siekhaus, Sophie A.O. Armitage, and Leila Masri for comments on the manuscript. \r\n","doi":"10.1016/j.cub.2012.11.034","year":"2013","month":"01","intvolume":"        23","publisher":"Cell Press","volume":23,"quality_controlled":"1","citation":{"short":"S. Tragust, B. Mitteregger, V. Barone, M. Konrad, L.V. Ugelvig, S. Cremer, Current Biology 23 (2013) 76–82.","ista":"Tragust S, Mitteregger B, Barone V, Konrad M, Ugelvig LV, Cremer S. 2013. Ants disinfect fungus-exposed brood by oral uptake and spread of their poison. Current Biology. 23(1), 76–82.","mla":"Tragust, Simon, et al. “Ants Disinfect Fungus-Exposed Brood by Oral Uptake and Spread of Their Poison.” <i>Current Biology</i>, vol. 23, no. 1, Cell Press, 2013, pp. 76–82, doi:<a href=\"https://doi.org/10.1016/j.cub.2012.11.034\">10.1016/j.cub.2012.11.034</a>.","apa":"Tragust, S., Mitteregger, B., Barone, V., Konrad, M., Ugelvig, L. V., &#38; Cremer, S. (2013). Ants disinfect fungus-exposed brood by oral uptake and spread of their poison. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2012.11.034\">https://doi.org/10.1016/j.cub.2012.11.034</a>","ieee":"S. Tragust, B. Mitteregger, V. Barone, M. Konrad, L. V. Ugelvig, and S. Cremer, “Ants disinfect fungus-exposed brood by oral uptake and spread of their poison,” <i>Current Biology</i>, vol. 23, no. 1. Cell Press, pp. 76–82, 2013.","chicago":"Tragust, Simon, Barbara Mitteregger, Vanessa Barone, Matthias Konrad, Line V Ugelvig, and Sylvia Cremer. “Ants Disinfect Fungus-Exposed Brood by Oral Uptake and Spread of Their Poison.” <i>Current Biology</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cub.2012.11.034\">https://doi.org/10.1016/j.cub.2012.11.034</a>.","ama":"Tragust S, Mitteregger B, Barone V, Konrad M, Ugelvig LV, Cremer S. Ants disinfect fungus-exposed brood by oral uptake and spread of their poison. <i>Current Biology</i>. 2013;23(1):76-82. doi:<a href=\"https://doi.org/10.1016/j.cub.2012.11.034\">10.1016/j.cub.2012.11.034</a>"},"page":"76 - 82","article_processing_charge":"No","ec_funded":1,"status":"public","_id":"2926","corr_author":"1","scopus_import":"1","department":[{"_id":"SyCr"},{"_id":"CaHe"}],"publist_id":"3811","related_material":{"record":[{"id":"9757","status":"public","relation":"research_data"},{"id":"961","status":"public","relation":"dissertation_contains"}]},"date_updated":"2026-04-08T14:22:39Z"},{"date_published":"2013-11-01T00:00:00Z","external_id":{"isi":["000326392500009"]},"publication":"Journal of Cell Science","abstract":[{"lang":"eng","text":"It is firmly established that interactions between neurons and glia are fundamental across species for the correct establishment of a functional brain. Here, we found that the glia of the Drosophila larval brain display an essential non-autonomous role during the development of the optic lobe. The optic lobe develops from neuroepithelial cells that proliferate by dividing symmetrically until they switch to asymmetric/differentiative divisions that generate neuroblasts. The proneural gene lethal of scute (l9sc) is transiently activated by the epidermal growth factor receptor (EGFR)-Ras signal transduction pathway at the leading edge of a proneural wave that sweeps from medial to lateral neuroepithelium, promoting this switch. This process is tightly regulated by the tissue-autonomous function within the neuroepithelium of multiple signaling pathways, including EGFR-Ras and Notch. This study shows that the Notch ligand Serrate (Ser) is expressed in the glia and it forms a complex in vivo with Notch and Canoe, which colocalize at the adherens junctions of neuroepithelial cells. This complex is crucial for interactions between glia and neuroepithelial cells during optic lobe development. Ser is tissue-autonomously required in the glia where it activates Notch to regulate its proliferation, and non-autonomously in the neuroepithelium where Ser induces Notch signaling to avoid the premature activation of the EGFR-Ras pathway and hence of L9sc. Interestingly, different Notch activity reporters showed very different expression patterns in the glia and in the neuroepithelium, suggesting the existence of tissue-specific factors that promote the expression of particular Notch target genes or/and a reporter response dependent on different thresholds of Notch signaling."}],"issue":"21","oa_version":"None","day":"01","author":[{"full_name":"Pérez Gómez, Raquel","last_name":"Pérez Gómez","first_name":"Raquel"},{"id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87","first_name":"Jana","last_name":"Slovakova","full_name":"Slovakova, Jana"},{"first_name":"Noemí","last_name":"Rives Quinto","full_name":"Rives Quinto, Noemí"},{"last_name":"Krejčí","full_name":"Krejčí, Alena","first_name":"Alena"},{"full_name":"Carmena, Ana","last_name":"Carmena","first_name":"Ana"}],"publication_status":"published","isi":1,"language":[{"iso":"eng"}],"type":"journal_article","title":"A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development","date_created":"2018-12-11T11:56:43Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2013","doi":"10.1242/jcs.125617","month":"11","publisher":"Company of Biologists","intvolume":"       126","citation":{"ieee":"R. Pérez Gómez, J. Slovakova, N. Rives Quinto, A. Krejčí, and A. Carmena, “A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development,” <i>Journal of Cell Science</i>, vol. 126, no. 21. Company of Biologists, pp. 4873–4884, 2013.","chicago":"Pérez Gómez, Raquel, Jana Slovakova, Noemí Rives Quinto, Alena Krejčí, and Ana Carmena. “A Serrate-Notch-Canoe Complex Mediates Essential Interactions between Glia and Neuroepithelial Cells during Drosophila Optic Lobe Development.” <i>Journal of Cell Science</i>. Company of Biologists, 2013. <a href=\"https://doi.org/10.1242/jcs.125617\">https://doi.org/10.1242/jcs.125617</a>.","ama":"Pérez Gómez R, Slovakova J, Rives Quinto N, Krejčí A, Carmena A. A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development. <i>Journal of Cell Science</i>. 2013;126(21):4873-4884. doi:<a href=\"https://doi.org/10.1242/jcs.125617\">10.1242/jcs.125617</a>","ista":"Pérez Gómez R, Slovakova J, Rives Quinto N, Krejčí A, Carmena A. 2013. A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development. Journal of Cell Science. 126(21), 4873–4884.","short":"R. Pérez Gómez, J. Slovakova, N. Rives Quinto, A. Krejčí, A. Carmena, Journal of Cell Science 126 (2013) 4873–4884.","apa":"Pérez Gómez, R., Slovakova, J., Rives Quinto, N., Krejčí, A., &#38; Carmena, A. (2013). A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development. <i>Journal of Cell Science</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.125617\">https://doi.org/10.1242/jcs.125617</a>","mla":"Pérez Gómez, Raquel, et al. “A Serrate-Notch-Canoe Complex Mediates Essential Interactions between Glia and Neuroepithelial Cells during Drosophila Optic Lobe Development.” <i>Journal of Cell Science</i>, vol. 126, no. 21, Company of Biologists, 2013, pp. 4873–84, doi:<a href=\"https://doi.org/10.1242/jcs.125617\">10.1242/jcs.125617</a>."},"volume":126,"quality_controlled":"1","article_processing_charge":"No","page":"4873 - 4884","_id":"2278","status":"public","department":[{"_id":"CaHe"}],"scopus_import":"1","publist_id":"4658","date_updated":"2025-09-29T14:26:53Z"},{"date_updated":"2026-03-09T14:56:18Z","publist_id":"4652","oa":1,"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"1403"}]},"scopus_import":"1","department":[{"_id":"CaHe"}],"status":"public","_id":"2282","corr_author":"1","page":"1405 - 1414","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"article_processing_charge":"No","quality_controlled":"1","volume":15,"citation":{"short":"P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, C.-P.J. Heisenberg, Nature Cell Biology 15 (2013) 1405–1414.","ista":"Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. 2013. Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. Nature Cell Biology. 15, 1405–1414.","mla":"Campinho, Pedro, et al. “Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell Biology</i>, vol. 15, Nature Publishing Group, 2013, pp. 1405–14, doi:<a href=\"https://doi.org/10.1038/ncb2869\">10.1038/ncb2869</a>.","apa":"Campinho, P., Behrndt, M., Ranft, J., Risler, T., Minc, N., &#38; Heisenberg, C.-P. J. (2013). Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2869\">https://doi.org/10.1038/ncb2869</a>","ieee":"P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, and C.-P. J. Heisenberg, “Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly,” <i>Nature Cell Biology</i>, vol. 15. Nature Publishing Group, pp. 1405–1414, 2013.","ama":"Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. <i>Nature Cell Biology</i>. 2013;15:1405-1414. doi:<a href=\"https://doi.org/10.1038/ncb2869\">10.1038/ncb2869</a>","chicago":"Campinho, Pedro, Martin Behrndt, Jonas Ranft, Thomas Risler, Nicolas Minc, and Carl-Philipp J Heisenberg. “Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2013. <a href=\"https://doi.org/10.1038/ncb2869\">https://doi.org/10.1038/ncb2869</a>."},"intvolume":"        15","publisher":"Nature Publishing Group","month":"11","acknowledgement":"This work was supported by the IST Austria and MPI-CBG ","doi":"10.1038/ncb2869","year":"2013","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:56:45Z","project":[{"grant_number":"I930-B20","name":"Control of Epithelial Cell Layer Spreading in Zebrafish","_id":"252ABD0A-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"type":"journal_article","title":"Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly","language":[{"iso":"eng"}],"isi":1,"publication_status":"published","author":[{"orcid":"0000-0002-8526-5416","full_name":"Campinho, Pedro","last_name":"Campinho","first_name":"Pedro","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Behrndt, Martin","last_name":"Behrndt","first_name":"Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jonas","full_name":"Ranft, Jonas","last_name":"Ranft"},{"full_name":"Risler, Thomas","last_name":"Risler","first_name":"Thomas"},{"first_name":"Nicolas","last_name":"Minc","full_name":"Minc, Nicolas"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"day":"10","oa_version":"Submitted Version","main_file_link":[{"url":"http://hal.upmc.fr/hal-00983313/","open_access":"1"}],"abstract":[{"lang":"eng","text":"Epithelial spreading is a common and fundamental aspect of various developmental and disease-related processes such as epithelial closure and wound healing. A key challenge for epithelial tissues undergoing spreading is to increase their surface area without disrupting epithelial integrity. Here we show that orienting cell divisions by tension constitutes an efficient mechanism by which the enveloping cell layer (EVL) releases anisotropic tension while undergoing spreading during zebrafish epiboly. The control of EVL cell-division orientation by tension involves cell elongation and requires myosin II activity to align the mitotic spindle with the main tension axis. We also found that in the absence of tension-oriented cell divisions and in the presence of increased tissue tension, EVL cells undergo ectopic fusions, suggesting that the reduction of tension anisotropy by oriented cell divisions is required to prevent EVL cells from fusing. We conclude that cell-division orientation by tension constitutes a key mechanism for limiting tension anisotropy and thus promoting tissue spreading during EVL epiboly."}],"publication":"Nature Cell Biology","date_published":"2013-11-10T00:00:00Z","external_id":{"isi":["000327944200005"]}},{"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817470/"}],"issue":"21","pmid":1,"oa_version":"Submitted Version","day":"04","author":[{"orcid":"0000-0002-8526-5416","full_name":"Campinho, Pedro","last_name":"Campinho","first_name":"Pedro","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"date_published":"2013-10-04T00:00:00Z","external_id":{"isi":["000326504900001"],"pmid":["24097062"]},"publication":"EMBO Journal","abstract":[{"lang":"eng","text":"The spatiotemporal control of cell divisions is a key factor in epithelial morphogenesis and patterning. Mao et al (2013) now describe how differential rates of proliferation within the Drosophila wing disc epithelium give rise to anisotropic tissue tension in peripheral/proximal regions of the disc. Such global tissue tension anisotropy in turn determines the orientation of cell divisions by controlling epithelial cell elongation."}],"date_created":"2018-12-11T11:56:46Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2013","doi":"10.1038/emboj.2013.225","publication_status":"published","isi":1,"language":[{"iso":"eng"}],"title":"The force and effect of cell proliferation","type":"journal_article","citation":{"ieee":"P. Campinho and C.-P. J. Heisenberg, “The force and effect of cell proliferation,” <i>EMBO Journal</i>, vol. 32, no. 21. Wiley-Blackwell, pp. 2783–2784, 2013.","ama":"Campinho P, Heisenberg C-PJ. The force and effect of cell proliferation. <i>EMBO Journal</i>. 2013;32(21):2783-2784. doi:<a href=\"https://doi.org/10.1038/emboj.2013.225\">10.1038/emboj.2013.225</a>","chicago":"Campinho, Pedro, and Carl-Philipp J Heisenberg. “The Force and Effect of Cell Proliferation.” <i>EMBO Journal</i>. Wiley-Blackwell, 2013. <a href=\"https://doi.org/10.1038/emboj.2013.225\">https://doi.org/10.1038/emboj.2013.225</a>.","short":"P. Campinho, C.-P.J. Heisenberg, EMBO Journal 32 (2013) 2783–2784.","ista":"Campinho P, Heisenberg C-PJ. 2013. The force and effect of cell proliferation. EMBO Journal. 32(21), 2783–2784.","mla":"Campinho, Pedro, and Carl-Philipp J. Heisenberg. “The Force and Effect of Cell Proliferation.” <i>EMBO Journal</i>, vol. 32, no. 21, Wiley-Blackwell, 2013, pp. 2783–84, doi:<a href=\"https://doi.org/10.1038/emboj.2013.225\">10.1038/emboj.2013.225</a>.","apa":"Campinho, P., &#38; Heisenberg, C.-P. J. (2013). The force and effect of cell proliferation. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2013.225\">https://doi.org/10.1038/emboj.2013.225</a>"},"volume":32,"quality_controlled":"1","article_processing_charge":"No","page":"2783 - 2784","month":"10","publisher":"Wiley-Blackwell","intvolume":"        32","oa":1,"publist_id":"4645","date_updated":"2025-09-29T14:23:43Z","corr_author":"1","_id":"2286","status":"public","department":[{"_id":"CaHe"}],"scopus_import":"1"},{"publication_status":"published","title":"Three functions of cadherins in cell adhesion","type":"journal_article","isi":1,"language":[{"iso":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:57:51Z","doi":"10.1016/j.cub.2013.06.019","year":"2013","publication":"Current Biology","external_id":{"pmid":["23885883"],"isi":["000322414200018"]},"date_published":"2013-07-22T00:00:00Z","file":[{"creator":"dernst","date_updated":"2020-07-14T12:45:41Z","date_created":"2019-01-24T15:40:22Z","content_type":"application/pdf","file_size":247320,"checksum":"6a424b2f007b41d4955a9135793b2162","file_id":"5881","file_name":"2013_CurrentBiology_Maitre.pdf","relation":"main_file","access_level":"open_access"}],"abstract":[{"lang":"eng","text":"Cadherins are transmembrane proteins that mediate cell–cell adhesion in animals. By regulating contact formation and stability, cadherins play a crucial role in tissue morphogenesis and homeostasis. Here, we review the three major  unctions of cadherins in cell–cell contact formation and stability. Two of those functions lead to a decrease in interfacial ension at the forming cell–cell contact, thereby promoting contact expansion — first, by providing adhesion tension that lowers interfacial tension at the cell–cell contact, and second, by signaling to the actomyosin cytoskeleton in order to reduce cortex tension and thus interfacial tension at the contact. The third function of cadherins in cell–cell contact formation is to stabilize the contact by resisting mechanical forces that pull on the contact."}],"pmid":1,"oa_version":"Published Version","issue":"14","author":[{"orcid":"0000-0002-3688-1474","first_name":"Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon","last_name":"Maître"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"day":"22","status":"public","corr_author":"1","_id":"2469","scopus_import":"1","department":[{"_id":"CaHe"}],"file_date_updated":"2020-07-14T12:45:41Z","ddc":["570"],"publist_id":"4433","oa":1,"date_updated":"2025-09-29T14:12:25Z","month":"07","intvolume":"        23","publisher":"Cell Press","quality_controlled":"1","volume":23,"citation":{"mla":"Maître, Jean-Léon, and Carl-Philipp J. Heisenberg. “Three Functions of Cadherins in Cell Adhesion.” <i>Current Biology</i>, vol. 23, no. 14, Cell Press, 2013, pp. R626–33, doi:<a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">10.1016/j.cub.2013.06.019</a>.","apa":"Maître, J.-L., &#38; Heisenberg, C.-P. J. (2013). Three functions of cadherins in cell adhesion. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">https://doi.org/10.1016/j.cub.2013.06.019</a>","short":"J.-L. Maître, C.-P.J. Heisenberg, Current Biology 23 (2013) R626–R633.","ista":"Maître J-L, Heisenberg C-PJ. 2013. Three functions of cadherins in cell adhesion. Current Biology. 23(14), R626–R633.","ama":"Maître J-L, Heisenberg C-PJ. Three functions of cadherins in cell adhesion. <i>Current Biology</i>. 2013;23(14):R626-R633. doi:<a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">10.1016/j.cub.2013.06.019</a>","chicago":"Maître, Jean-Léon, and Carl-Philipp J Heisenberg. “Three Functions of Cadherins in Cell Adhesion.” <i>Current Biology</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">https://doi.org/10.1016/j.cub.2013.06.019</a>.","ieee":"J.-L. Maître and C.-P. J. Heisenberg, “Three functions of cadherins in cell adhesion,” <i>Current Biology</i>, vol. 23, no. 14. Cell Press, pp. R626–R633, 2013."},"has_accepted_license":"1","page":"R626 - R633","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No"},{"publication_identifier":{"issn":["2663-337X"]},"year":"2013","date_updated":"2026-04-09T14:34:43Z","alternative_title":["ISTA Thesis"],"date_created":"2018-12-11T11:51:50Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publist_id":"5801","department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"title":"Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading","type":"dissertation","degree_awarded":"PhD","_id":"1406","corr_author":"1","publication_status":"published","status":"public","day":"01","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"article_processing_charge":"No","page":"123","author":[{"orcid":"0000-0002-8526-5416","full_name":"Campinho, Pedro","last_name":"Campinho","first_name":"Pedro","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87"}],"citation":{"ieee":"P. Campinho, “Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading,” Institute of Science and Technology Austria, 2013.","ama":"Campinho P. Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading. 2013.","chicago":"Campinho, Pedro. “Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading.” Institute of Science and Technology Austria, 2013.","ista":"Campinho P. 2013. Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading. Institute of Science and Technology Austria.","short":"P. Campinho, Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading, Institute of Science and Technology Austria, 2013.","mla":"Campinho, Pedro. <i>Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading</i>. Institute of Science and Technology Austria, 2013.","apa":"Campinho, P. (2013). <i>Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading</i>. Institute of Science and Technology Austria."},"oa_version":"None","publisher":"Institute of Science and Technology Austria","supervisor":[{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"abstract":[{"text":"Epithelial spreading is a critical part of various developmental and wound repair processes. Here we use zebrafish epiboly as a model system to study the cellular and molecular mechanisms underlying the spreading of epithelial sheets. During zebrafish epiboly the enveloping cell layer (EVL), a simple squamous epithelium, spreads over the embryo to eventually cover the entire yolk cell by the end of gastrulation. The EVL leading edge is anchored through tight junctions to the yolk syncytial layer (YSL), where directly adjacent to the EVL margin a contractile actomyosin ring is formed that is thought to drive EVL epiboly. The prevalent view in the field was that the contractile ring exerts a pulling force on the EVL margin, which pulls the EVL towards the vegetal pole. However, how this force is generated and how it affects EVL morphology still remains elusive. Moreover, the cellular mechanisms mediating the increase in EVL surface area, while maintaining tissue integrity and function are still unclear. Here we show that the YSL actomyosin ring pulls on the EVL margin by two distinct force-generating mechanisms. One mechanism is based on contraction of the ring around its circumference, as previously proposed. The second mechanism is based on actomyosin retrogade flows, generating force through resistance against the substrate. The latter can function at any epiboly stage even in situations where the contraction-based mechanism is unproductive. Additionally, we demonstrate that during epiboly the EVL is subjected to anisotropic tension, which guides the orientation of EVL cell division along the main axis (animal-vegetal) of tension. The influence of tension in cell division orientation involves cell elongation and requires myosin-2 activity for proper spindle alignment. Strikingly, we reveal that tension-oriented cell divisions release anisotropic tension within the EVL and that in the absence of such divisions, EVL cells undergo ectopic fusions. We conclude that forces applied to the EVL by the action of the YSL actomyosin ring generate a tension anisotropy in the EVL that orients cell divisions, which in turn limit tissue tension increase thereby facilitating tissue spreading.","lang":"eng"}],"date_published":"2013-10-01T00:00:00Z","month":"10","OA_place":"publisher"}]