[{"author":[{"last_name":"Behrndt","full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin"},{"last_name":"Salbreux","first_name":"Guillaume","full_name":"Salbreux, Guillaume"},{"id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","full_name":"Campinho, Pedro","first_name":"Pedro","last_name":"Campinho","orcid":"0000-0002-8526-5416"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild"},{"last_name":"Oswald","first_name":"Felix","full_name":"Oswald, Felix"},{"last_name":"Roensch","full_name":"Roensch, Julia","id":"4220E59C-F248-11E8-B48F-1D18A9856A87","first_name":"Julia"},{"full_name":"Grill, Stephan","first_name":"Stephan","last_name":"Grill"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}],"article_type":"original","publication_status":"published","project":[{"name":"Control of Epithelial Cell Layer Spreading in Zebrafish","call_identifier":"FWF","_id":"252ABD0A-B435-11E9-9278-68D0E5697425","grant_number":"I930-B20"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"status":"public","date_published":"2012-10-12T00:00:00Z","scopus_import":"1","abstract":[{"lang":"eng","text":"Contractile actomyosin rings drive various fundamental morphogenetic processes ranging from cytokinesis to wound healing. Actomyosin rings are generally thought to function by circumferential contraction. Here, we show that the spreading of the enveloping cell layer (EVL) over the yolk cell during zebrafish gastrulation is driven by a contractile actomyosin ring. In contrast to previous suggestions, we find that this ring functions not only by circumferential contraction but also by a flow-friction mechanism. This generates a pulling force through resistance against retrograde actomyosin flow. EVL spreading proceeds normally in situations where circumferential contraction is unproductive, indicating that the flow-friction mechanism is sufficient. Thus, actomyosin rings can function in epithelial morphogenesis through a combination of cable-constriction and flow-friction mechanisms."}],"acknowledgement":"We are grateful to M. Sixt, T. Bollenbach, and E. Martin-Blanco for advice and the service facilities of the IST Austria and MPI-CBG for continuous help. M.B., G.S., S.W.G., and C.-P.H. synergistically and equally developed the presented ideas and the experimental and theoretical approaches. M.B. and P.C. performed the experiments; G.S. developed the theory; and R.H., F.O., and J.R. contributed to the experimental work. This work was supported by a grant from the Fonds zur Förderung der wissenschaftlichen Forschung (FWF) and the Deutsche Forschungsgemeinschaft (DFG) (I930-B20) to C.-P.H., S.W.G., and G.S.","OA_type":"closed access","article_processing_charge":"No","month":"10","pmid":1,"oa_version":"None","_id":"2950","issue":"6104","year":"2012","title":"Forces driving epithelial spreading in zebrafish gastrulation","department":[{"_id":"CaHe"},{"_id":"Bio"}],"date_created":"2018-12-11T12:00:30Z","acknowledged_ssus":[{"_id":"SSU"}],"language":[{"iso":"eng"}],"quality_controlled":"1","intvolume":"       338","corr_author":"1","external_id":{"isi":["000309712300046"],"pmid":["23066079"]},"date_updated":"2026-03-09T14:56:18Z","publication":"Science","publist_id":"3778","volume":338,"citation":{"ieee":"M. Behrndt <i>et al.</i>, “Forces driving epithelial spreading in zebrafish gastrulation,” <i>Science</i>, vol. 338, no. 6104. American Association for the Advancement of Science, pp. 257–260, 2012.","ista":"Behrndt M, Salbreux G, Campinho P, Hauschild R, Oswald F, Roensch J, Grill S, Heisenberg C-PJ. 2012. Forces driving epithelial spreading in zebrafish gastrulation. Science. 338(6104), 257–260.","apa":"Behrndt, M., Salbreux, G., Campinho, P., Hauschild, R., Oswald, F., Roensch, J., … Heisenberg, C.-P. J. (2012). Forces driving epithelial spreading in zebrafish gastrulation. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1224143\">https://doi.org/10.1126/science.1224143</a>","mla":"Behrndt, Martin, et al. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.” <i>Science</i>, vol. 338, no. 6104, American Association for the Advancement of Science, 2012, pp. 257–60, doi:<a href=\"https://doi.org/10.1126/science.1224143\">10.1126/science.1224143</a>.","short":"M. Behrndt, G. Salbreux, P. Campinho, R. Hauschild, F. Oswald, J. Roensch, S. Grill, C.-P.J. Heisenberg, Science 338 (2012) 257–260.","chicago":"Behrndt, Martin, Guillaume Salbreux, Pedro Campinho, Robert Hauschild, Felix Oswald, Julia Roensch, Stephan Grill, and Carl-Philipp J Heisenberg. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.” <i>Science</i>. American Association for the Advancement of Science, 2012. <a href=\"https://doi.org/10.1126/science.1224143\">https://doi.org/10.1126/science.1224143</a>.","ama":"Behrndt M, Salbreux G, Campinho P, et al. Forces driving epithelial spreading in zebrafish gastrulation. <i>Science</i>. 2012;338(6104):257-260. doi:<a href=\"https://doi.org/10.1126/science.1224143\">10.1126/science.1224143</a>"},"type":"journal_article","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"1403"}]},"doi":"10.1126/science.1224143","day":"12","publisher":"American Association for the Advancement of Science","page":"257 - 260"},{"oa_version":"None","issue":"6104","_id":"2951","year":"2012","title":"Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells","article_processing_charge":"No","month":"10","status":"public","abstract":[{"text":"Differential cell adhesion and cortex tension are thought to drive cell sorting by controlling cell-cell contact formation. Here, we show that cell adhesion and cortex tension have different mechanical functions in controlling progenitor cell-cell contact formation and sorting during zebrafish gastrulation. Cortex tension controls cell-cell contact expansion by modulating interfacial tension at the contact. By contrast, adhesion has little direct function in contact expansion, but instead is needed to mechanically couple the cortices of adhering cells at their contacts, allowing cortex tension to control contact expansion. The coupling function of adhesion is mediated by E-cadherin and limited by the mechanical anchoring of E-cadherin to the cortex. Thus, cell adhesion provides the mechanical scaffold for cell cortex tension to drive cell sorting during gastrulation.","lang":"eng"}],"scopus_import":"1","date_published":"2012-10-12T00:00:00Z","author":[{"orcid":"0000-0002-3688-1474","last_name":"Maître","first_name":"Jean-Léon","full_name":"Maître, Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Hélène","full_name":"Berthoumieux, Hélène","last_name":"Berthoumieux"},{"orcid":"0000-0003-4761-5996","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel","first_name":"Gabriel"},{"first_name":"Guillaume","full_name":"Salbreux, Guillaume","last_name":"Salbreux"},{"full_name":"Julicher, Frank","first_name":"Frank","last_name":"Julicher"},{"last_name":"Paluch","first_name":"Ewa","full_name":"Paluch, Ewa"},{"last_name":"Heisenberg","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"publication_status":"published","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","volume":338,"citation":{"chicago":"Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux, Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” <i>Science</i>. American Association for the Advancement of Science, 2012. <a href=\"https://doi.org/10.1126/science.1225399\">https://doi.org/10.1126/science.1225399</a>.","ama":"Maître J-L, Berthoumieux H, Krens G, et al. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. <i>Science</i>. 2012;338(6104):253-256. doi:<a href=\"https://doi.org/10.1126/science.1225399\">10.1126/science.1225399</a>","mla":"Maître, Jean-Léon, et al. “Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” <i>Science</i>, vol. 338, no. 6104, American Association for the Advancement of Science, 2012, pp. 253–56, doi:<a href=\"https://doi.org/10.1126/science.1225399\">10.1126/science.1225399</a>.","ista":"Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg C-PJ. 2012. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. Science. 338(6104), 253–256.","apa":"Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch, E., &#38; Heisenberg, C.-P. J. (2012). Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1225399\">https://doi.org/10.1126/science.1225399</a>","ieee":"J.-L. Maître <i>et al.</i>, “Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells,” <i>Science</i>, vol. 338, no. 6104. American Association for the Advancement of Science, pp. 253–256, 2012.","short":"J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch, C.-P.J. Heisenberg, Science 338 (2012) 253–256."},"type":"journal_article","doi":"10.1126/science.1225399","day":"12","publisher":"American Association for the Advancement of Science","page":"253 - 256","publication":"Science","publist_id":"3777","quality_controlled":"1","language":[{"iso":"eng"}],"intvolume":"       338","corr_author":"1","external_id":{"isi":["000309712300045"]},"date_updated":"2025-09-30T08:10:36Z","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T12:00:31Z","acknowledged_ssus":[{"_id":"SSU"}]},{"title":"Convergent extension Using collective cell migration and cell intercalation to shape embryos","oa_version":"None","_id":"2952","issue":"21","year":"2012","month":"11","article_processing_charge":"No","acknowledgement":"M.T. is supported by the UK Medical Research Council (MRC) and Royal Society and C.-P.H. by the Fonds zur Förderung der wissenschaftlichen Forschung (FWF), Deutsche Forschungsgemeinschaft (DFG) and Institute of Science and Technology Austria. ","status":"public","scopus_import":"1","date_published":"2012-11-01T00:00:00Z","abstract":[{"lang":"eng","text":"Body axis elongation represents a common and fundamental morphogenetic process in development. A key mechanism triggering body axis elongation without additional growth is convergent extension (CE), whereby a tissue undergoes simultaneous narrowing and extension. Both collective cell migration and cell intercalation are thought to drive CE and are used to different degrees in various species as they elongate their body axis. Here, we provide an overview of CE as a general strategy for body axis elongation and discuss conserved and divergent mechanisms underlying CE among different species."}],"isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"first_name":"Masazumi","full_name":"Tada, Masazumi","last_name":"Tada"},{"first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}],"publication_status":"published","type":"journal_article","doi":"10.1242/dev.073007","day":"01","publisher":"Company of Biologists","page":"3897 - 3904","volume":139,"citation":{"ama":"Tada M, Heisenberg C-PJ. Convergent extension Using collective cell migration and cell intercalation to shape embryos. <i>Development</i>. 2012;139(21):3897-3904. doi:<a href=\"https://doi.org/10.1242/dev.073007\">10.1242/dev.073007</a>","chicago":"Tada, Masazumi, and Carl-Philipp J Heisenberg. “Convergent Extension Using Collective Cell Migration and Cell Intercalation to Shape Embryos.” <i>Development</i>. Company of Biologists, 2012. <a href=\"https://doi.org/10.1242/dev.073007\">https://doi.org/10.1242/dev.073007</a>.","short":"M. Tada, C.-P.J. Heisenberg, Development 139 (2012) 3897–3904.","apa":"Tada, M., &#38; Heisenberg, C.-P. J. (2012). Convergent extension Using collective cell migration and cell intercalation to shape embryos. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.073007\">https://doi.org/10.1242/dev.073007</a>","ista":"Tada M, Heisenberg C-PJ. 2012. Convergent extension Using collective cell migration and cell intercalation to shape embryos. Development. 139(21), 3897–3904.","mla":"Tada, Masazumi, and Carl-Philipp J. Heisenberg. “Convergent Extension Using Collective Cell Migration and Cell Intercalation to Shape Embryos.” <i>Development</i>, vol. 139, no. 21, Company of Biologists, 2012, pp. 3897–904, doi:<a href=\"https://doi.org/10.1242/dev.073007\">10.1242/dev.073007</a>.","ieee":"M. Tada and C.-P. J. Heisenberg, “Convergent extension Using collective cell migration and cell intercalation to shape embryos,” <i>Development</i>, vol. 139, no. 21. Company of Biologists, pp. 3897–3904, 2012."},"publist_id":"3776","publication":"Development","corr_author":"1","external_id":{"isi":["000309701300002"]},"date_updated":"2025-09-30T08:10:08Z","language":[{"iso":"eng"}],"quality_controlled":"1","intvolume":"       139","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T12:00:31Z"},{"isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_status":"published","author":[{"last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"},{"last_name":"Fässler","full_name":"Fässler, Reinhard","first_name":"Reinhard"}],"date_published":"2012-10-01T00:00:00Z","scopus_import":"1","status":"public","month":"10","article_processing_charge":"No","title":"Cell-cell adhesion and extracellular matrix diversity counts","_id":"2953","issue":"5","year":"2012","oa_version":"None","date_created":"2018-12-11T12:00:31Z","department":[{"_id":"CaHe"}],"date_updated":"2025-09-30T08:09:10Z","corr_author":"1","external_id":{"isi":["000310943300001"]},"language":[{"iso":"eng"}],"quality_controlled":"1","intvolume":"        24","publist_id":"3773","publication":"Current Opinion in Cell Biology","publisher":"Elsevier","day":"01","doi":"10.1016/j.ceb.2012.09.002","page":"559 - 561","type":"journal_article","citation":{"apa":"Heisenberg, C.-P. J., &#38; Fässler, R. (2012). Cell-cell adhesion and extracellular matrix diversity counts. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2012.09.002\">https://doi.org/10.1016/j.ceb.2012.09.002</a>","ista":"Heisenberg C-PJ, Fässler R. 2012. Cell-cell adhesion and extracellular matrix diversity counts. Current Opinion in Cell Biology. 24(5), 559–561.","mla":"Heisenberg, Carl-Philipp J., and Reinhard Fässler. “Cell-Cell Adhesion and Extracellular Matrix Diversity Counts.” <i>Current Opinion in Cell Biology</i>, vol. 24, no. 5, Elsevier, 2012, pp. 559–61, doi:<a href=\"https://doi.org/10.1016/j.ceb.2012.09.002\">10.1016/j.ceb.2012.09.002</a>.","ieee":"C.-P. J. Heisenberg and R. Fässler, “Cell-cell adhesion and extracellular matrix diversity counts,” <i>Current Opinion in Cell Biology</i>, vol. 24, no. 5. Elsevier, pp. 559–561, 2012.","short":"C.-P.J. Heisenberg, R. Fässler, Current Opinion in Cell Biology 24 (2012) 559–561.","chicago":"Heisenberg, Carl-Philipp J, and Reinhard Fässler. “Cell-Cell Adhesion and Extracellular Matrix Diversity Counts.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2012. <a href=\"https://doi.org/10.1016/j.ceb.2012.09.002\">https://doi.org/10.1016/j.ceb.2012.09.002</a>.","ama":"Heisenberg C-PJ, Fässler R. Cell-cell adhesion and extracellular matrix diversity counts. <i>Current Opinion in Cell Biology</i>. 2012;24(5):559-561. doi:<a href=\"https://doi.org/10.1016/j.ceb.2012.09.002\">10.1016/j.ceb.2012.09.002</a>"},"volume":24},{"isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_status":"published","author":[{"last_name":"Behrndt","first_name":"Martin","full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"date_published":"2012-01-17T00:00:00Z","abstract":[{"text":"How cells orchestrate their behavior during collective migration is a long-standing question. Using magnetic tweezers to apply mechanical stimuli to Xenopus mesendoderm cells, Weber etal. (2012) now reveal, in this issue of Developmental Cell, a cadherin-mediated mechanosensitive response that promotes cell polarization and movement persistence during the collective mesendoderm migration in gastrulation.","lang":"eng"}],"scopus_import":"1","status":"public","month":"01","article_processing_charge":"No","title":"Spurred by resistance mechanosensation in collective migration","year":"2012","_id":"3245","issue":"1","oa_version":"None","date_created":"2018-12-11T12:02:14Z","department":[{"_id":"CaHe"}],"date_updated":"2025-09-30T07:48:41Z","external_id":{"isi":["000299365800002"]},"corr_author":"1","intvolume":"        22","language":[{"iso":"eng"}],"quality_controlled":"1","publist_id":"3426","publication":"Developmental Cell","page":"3 - 4","doi":"10.1016/j.devcel.2011.12.018","day":"17","publisher":"Cell Press","type":"journal_article","citation":{"short":"M. Behrndt, C.-P.J. Heisenberg, Developmental Cell 22 (2012) 3–4.","ieee":"M. Behrndt and C.-P. J. Heisenberg, “Spurred by resistance mechanosensation in collective migration,” <i>Developmental Cell</i>, vol. 22, no. 1. Cell Press, pp. 3–4, 2012.","ista":"Behrndt M, Heisenberg C-PJ. 2012. Spurred by resistance mechanosensation in collective migration. Developmental Cell. 22(1), 3–4.","apa":"Behrndt, M., &#38; Heisenberg, C.-P. J. (2012). Spurred by resistance mechanosensation in collective migration. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2011.12.018\">https://doi.org/10.1016/j.devcel.2011.12.018</a>","mla":"Behrndt, Martin, and Carl-Philipp J. Heisenberg. “Spurred by Resistance Mechanosensation in Collective Migration.” <i>Developmental Cell</i>, vol. 22, no. 1, Cell Press, 2012, pp. 3–4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2011.12.018\">10.1016/j.devcel.2011.12.018</a>.","ama":"Behrndt M, Heisenberg C-PJ. Spurred by resistance mechanosensation in collective migration. <i>Developmental Cell</i>. 2012;22(1):3-4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2011.12.018\">10.1016/j.devcel.2011.12.018</a>","chicago":"Behrndt, Martin, and Carl-Philipp J Heisenberg. “Spurred by Resistance Mechanosensation in Collective Migration.” <i>Developmental Cell</i>. Cell Press, 2012. <a href=\"https://doi.org/10.1016/j.devcel.2011.12.018\">https://doi.org/10.1016/j.devcel.2011.12.018</a>."},"volume":22},{"article_processing_charge":"No","month":"02","oa_version":"None","year":"2012","issue":"1","_id":"3246","title":"Cell adhesion in embryo morphogenesis","author":[{"full_name":"Barone, Vanessa","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","orcid":"0000-0003-2676-3367","last_name":"Barone"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"publication_status":"published","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","abstract":[{"text":"Visualizing and analyzing shape changes at various scales, ranging from single molecules to whole organisms, are essential for understanding complex morphogenetic processes, such as early embryonic development. Embryo morphogenesis relies on the interplay between different tissues, the properties of which are again determined by the interaction between their constituent cells. Cell interactions, on the other hand, are controlled by various molecules, such as signaling and adhesion molecules, which in order to exert their functions need to be spatiotemporally organized within and between the interacting cells. In this review, we will focus on the role of cell adhesion functioning at different scales to organize cell, tissue and embryo morphogenesis. We will specifically ask how the subcellular distribution of adhesion molecules controls the formation of cell-cell contacts, how cell-cell contacts determine tissue shape, and how tissue interactions regulate embryo morphogenesis.","lang":"eng"}],"date_published":"2012-02-01T00:00:00Z","scopus_import":"1","acknowledgement":"This review comes from a themed issue on Cell structure and dynamics Edited by Jason Swedlow and Gaudenz Danuser","publication":"Current Opinion in Cell Biology","publist_id":"3423","volume":24,"citation":{"short":"V. Barone, C.-P.J. Heisenberg, Current Opinion in Cell Biology 24 (2012) 148–153.","ista":"Barone V, Heisenberg C-PJ. 2012. Cell adhesion in embryo morphogenesis. Current Opinion in Cell Biology. 24(1), 148–153.","apa":"Barone, V., &#38; Heisenberg, C.-P. J. (2012). Cell adhesion in embryo morphogenesis. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2011.11.006\">https://doi.org/10.1016/j.ceb.2011.11.006</a>","mla":"Barone, Vanessa, and Carl-Philipp J. Heisenberg. “Cell Adhesion in Embryo Morphogenesis.” <i>Current Opinion in Cell Biology</i>, vol. 24, no. 1, Elsevier, 2012, pp. 148–53, doi:<a href=\"https://doi.org/10.1016/j.ceb.2011.11.006\">10.1016/j.ceb.2011.11.006</a>.","ieee":"V. Barone and C.-P. J. Heisenberg, “Cell adhesion in embryo morphogenesis,” <i>Current Opinion in Cell Biology</i>, vol. 24, no. 1. Elsevier, pp. 148–153, 2012.","ama":"Barone V, Heisenberg C-PJ. Cell adhesion in embryo morphogenesis. <i>Current Opinion in Cell Biology</i>. 2012;24(1):148-153. doi:<a href=\"https://doi.org/10.1016/j.ceb.2011.11.006\">10.1016/j.ceb.2011.11.006</a>","chicago":"Barone, Vanessa, and Carl-Philipp J Heisenberg. “Cell Adhesion in Embryo Morphogenesis.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2012. <a href=\"https://doi.org/10.1016/j.ceb.2011.11.006\">https://doi.org/10.1016/j.ceb.2011.11.006</a>."},"related_material":{"record":[{"id":"961","relation":"dissertation_contains","status":"public"}]},"type":"journal_article","page":"148 - 153","publisher":"Elsevier","doi":"10.1016/j.ceb.2011.11.006","day":"01","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T12:02:14Z","intvolume":"        24","language":[{"iso":"eng"}],"quality_controlled":"1","external_id":{"isi":["000302206900020"]},"corr_author":"1","date_updated":"2026-04-08T14:22:39Z"},{"page":"714 - 724","publisher":"Bentham Science Publishers","day":"01","doi":"10.2174/138920311798841753","type":"journal_article","citation":{"apa":"Ruprecht, V., Axmann, M., Wieser, S., &#38; Schuetz, G. (2011). What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>","ista":"Ruprecht V, Axmann M, Wieser S, Schuetz G. 2011. What can we learn from single molecule trajectories? Current Protein &#38; Peptide Science. 12(8), 714–724.","mla":"Ruprecht, Verena, et al. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8, Bentham Science Publishers, 2011, pp. 714–24, doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>.","ieee":"V. Ruprecht, M. Axmann, S. Wieser, and G. Schuetz, “What can we learn from single molecule trajectories?,” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8. Bentham Science Publishers, pp. 714–724, 2011.","short":"V. Ruprecht, M. Axmann, S. Wieser, G. Schuetz, Current Protein &#38; Peptide Science 12 (2011) 714–724.","chicago":"Ruprecht, Verena, Markus Axmann, Stefan Wieser, and Gerhard Schuetz. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers, 2011. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>.","ama":"Ruprecht V, Axmann M, Wieser S, Schuetz G. What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. 2011;12(8):714-724. doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>"},"volume":12,"publist_id":"3358","publication":"Current Protein & Peptide Science","date_updated":"2025-09-30T09:21:31Z","external_id":{"isi":["000299672600005"]},"intvolume":"        12","quality_controlled":"1","language":[{"iso":"eng"}],"date_created":"2018-12-11T12:02:28Z","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"title":"What can we learn from single molecule trajectories?","year":"2011","_id":"3287","issue":"8","oa_version":"None","month":"12","article_processing_charge":"No","scopus_import":"1","date_published":"2011-12-01T00:00:00Z","abstract":[{"text":"Diffusing membrane constituents are constantly exposed to a variety of forces that influence their stochastic path. Single molecule experiments allow for resolving trajectories at extremely high spatial and temporal accuracy, thereby offering insights into en route interactions of the tracer. In this review we discuss approaches to derive information about the underlying processes, based on single molecule tracking experiments. In particular, we focus on a new versatile way to analyze single molecule diffusion in the absence of a full analytical treatment. The method is based on comprehensive comparison of an experimental data set against the hypothetical outcome of multiple experiments performed on the computer. Since Monte Carlo simulations can be easily and rapidly performed even on state-of-the-art PCs, our method provides a simple way for testing various - even complicated - diffusion models. We describe the new method in detail, and show the applicability on two specific examples: firstly, kinetic rate constants can be derived for the transient interaction of mobile membrane proteins; secondly, residence time and corral size can be extracted for confined diffusion.","lang":"eng"}],"status":"public","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_status":"published","author":[{"orcid":"0000-0003-4088-8633","last_name":"Ruprecht","first_name":"Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena"},{"last_name":"Axmann","full_name":"Axmann, Markus","first_name":"Markus"},{"last_name":"Wieser","orcid":"0000-0002-2670-2217","first_name":"Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan"},{"first_name":"Gerhard","full_name":"Schuetz, Gerhard","last_name":"Schuetz"}]},{"type":"journal_article","doi":"10.1371/journal.pone.0022458","publisher":"Public Library of Science","day":"22","volume":6,"citation":{"ama":"Smutny M, Wu S, Gomez G, Mangold S, Yap A, Hamilton N. Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens. <i>PLoS One</i>. 2011;6(7). doi:<a href=\"https://doi.org/10.1371/journal.pone.0022458\">10.1371/journal.pone.0022458</a>","chicago":"Smutny, Michael, Selwin Wu, Guillermo Gomez, Sabine Mangold, Alpha Yap, and Nicholas Hamilton. “Multicomponent Analysis of Junctional Movements Regulated by Myosin II Isoforms at the Epithelial Zonula Adherens.” <i>PLoS One</i>. Public Library of Science, 2011. <a href=\"https://doi.org/10.1371/journal.pone.0022458\">https://doi.org/10.1371/journal.pone.0022458</a>.","short":"M. Smutny, S. Wu, G. Gomez, S. Mangold, A. Yap, N. Hamilton, PLoS One 6 (2011).","ieee":"M. Smutny, S. Wu, G. Gomez, S. Mangold, A. Yap, and N. Hamilton, “Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens,” <i>PLoS One</i>, vol. 6, no. 7. Public Library of Science, 2011.","apa":"Smutny, M., Wu, S., Gomez, G., Mangold, S., Yap, A., &#38; Hamilton, N. (2011). Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0022458\">https://doi.org/10.1371/journal.pone.0022458</a>","ista":"Smutny M, Wu S, Gomez G, Mangold S, Yap A, Hamilton N. 2011. Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens. PLoS One. 6(7).","mla":"Smutny, Michael, et al. “Multicomponent Analysis of Junctional Movements Regulated by Myosin II Isoforms at the Epithelial Zonula Adherens.” <i>PLoS One</i>, vol. 6, no. 7, Public Library of Science, 2011, doi:<a href=\"https://doi.org/10.1371/journal.pone.0022458\">10.1371/journal.pone.0022458</a>."},"publist_id":"3357","publication":"PLoS One","external_id":{"isi":["000293097300049"]},"date_updated":"2025-09-30T09:26:01Z","has_accepted_license":"1","intvolume":"         6","quality_controlled":"1","language":[{"iso":"eng"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"department":[{"_id":"CaHe"}],"file_date_updated":"2020-07-14T12:46:06Z","date_created":"2018-12-11T12:02:28Z","title":"Multicomponent analysis of junctional movements regulated by Myosin II isoforms at the epithelial zonula adherens","oa_version":"Published Version","year":"2011","issue":"7","_id":"3288","month":"07","article_processing_charge":"No","acknowledgement":"his work was funded by the National Health and Medical Research Council (NHMRC) of Australia. M.S. was an Erwin Schroedinger postdoctoral fellow of the Austrian Science Fund (FWF), S.K.W. is supported by a UQ International Research Tuition Award and Research Scholarship, S.M .by an ANZ Trustees PhD Scholarship. A.S.Y. is a Research Fellow of the NHMRC. Confocal imaging was performed at the Australian Cancer Research Foundation (ACRF) Cancer Biology Imaging Centre at the Institute for Molecular Bioscience, established with the generous support of the ACRF.","ddc":["570"],"status":"public","date_published":"2011-07-22T00:00:00Z","abstract":[{"lang":"eng","text":"The zonula adherens (ZA) of epithelial cells is a site of cell-cell adhesion where cellular forces are exerted and resisted. Increasing evidence indicates that E-cadherin adhesion molecules at the ZA serve to sense force applied on the junctions and coordinate cytoskeletal responses to those forces. Efforts to understand the role that cadherins play in mechanotransduction have been limited by the lack of assays to measure the impact of forces on the ZA. In this study we used 4D imaging of GFP-tagged E-cadherin to analyse the movement of the ZA. Junctions in confluent epithelial monolayers displayed prominent movements oriented orthogonal (perpendicular) to the ZA itself. Two components were identified in these movements: a relatively slow unidirectional (translational) component that could be readily fitted by least-squares regression analysis, upon which were superimposed more rapid oscillatory movements. Myosin IIB was a dominant factor responsible for driving the unilateral translational movements. In contrast, frequency spectrum analysis revealed that depletion of Myosin IIA increased the power of the oscillatory movements. This implies that Myosin IIA may serve to dampen oscillatory movements of the ZA. This extends our recent analysis of Myosin II at the ZA to demonstrate that Myosin IIA and Myosin IIB make distinct contributions to junctional movement at the ZA."}],"oa":1,"file":[{"date_updated":"2020-07-14T12:46:06Z","file_name":"2011_PLOS_Smutny.PDF","checksum":"57a5eb11dd05241c48c44f492b3ec3ac","relation":"main_file","file_size":1984567,"file_id":"6399","access_level":"open_access","date_created":"2019-05-10T10:51:43Z","creator":"dernst","content_type":"application/pdf"}],"isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"last_name":"Smutny","orcid":"0000-0002-5920-9090","first_name":"Michael","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","full_name":"Smutny, Michael"},{"full_name":"Wu, Selwin","first_name":"Selwin","last_name":"Wu"},{"first_name":"Guillermo","full_name":"Gomez, Guillermo","last_name":"Gomez"},{"first_name":"Sabine","full_name":"Mangold, Sabine","last_name":"Mangold"},{"last_name":"Yap","full_name":"Yap, Alpha","first_name":"Alpha"},{"last_name":"Hamilton","full_name":"Hamilton, Nicholas","first_name":"Nicholas"}],"publication_status":"published"},{"article_processing_charge":"No","month":"01","pmid":1,"oa_version":"Submitted Version","year":"2011","_id":"3368","issue":"3","title":"Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants","author":[{"first_name":"Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel","last_name":"Krens","orcid":"0000-0003-4761-5996"},{"last_name":"Möllmert","first_name":"Stephanie","id":"260FD49C-E911-11E9-B5EA-D9538404589B","full_name":"Möllmert, Stephanie"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}],"publication_status":"published","oa":1,"isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","scopus_import":"1","abstract":[{"lang":"eng","text":"Tissue surface tension (TST) is an important mechanical property influencing cell sorting and tissue envelopment. The study by Manning et al. (1) reported on a mathematical model describing TST on the basis of the balance between adhesive and tensile properties of the constituent cells. The model predicts that, in high-adhesion cell aggregates, surface cells will be stretched to maintain the same area of cell–cell contact as interior bulk cells, resulting in an elongated and flattened cell shape. The authors (1) observed flat and elongated cells at the surface of high-adhesion zebrafish germ-layer explants, which they argue are undifferentiated stretched germ-layer progenitor cells, and they use this observation as a validation of their model."}],"date_published":"2011-01-18T00:00:00Z","publication":"PNAS","publist_id":"3244","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024655"}],"volume":108,"citation":{"chicago":"Krens, Gabriel, Stephanie Möllmert, and Carl-Philipp J Heisenberg. “Enveloping Cell Layer Differentiation at the Surface of Zebrafish Germ Layer Tissue Explants.” <i>PNAS</i>. National Academy of Sciences, 2011. <a href=\"https://doi.org/10.1073/pnas.1010767108\">https://doi.org/10.1073/pnas.1010767108</a>.","ama":"Krens G, Möllmert S, Heisenberg C-PJ. Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. <i>PNAS</i>. 2011;108(3):E9-E10. doi:<a href=\"https://doi.org/10.1073/pnas.1010767108\">10.1073/pnas.1010767108</a>","ieee":"G. Krens, S. Möllmert, and C.-P. J. Heisenberg, “Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants,” <i>PNAS</i>, vol. 108, no. 3. National Academy of Sciences, pp. E9–E10, 2011.","apa":"Krens, G., Möllmert, S., &#38; Heisenberg, C.-P. J. (2011). Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1010767108\">https://doi.org/10.1073/pnas.1010767108</a>","mla":"Krens, Gabriel, et al. “Enveloping Cell Layer Differentiation at the Surface of Zebrafish Germ Layer Tissue Explants.” <i>PNAS</i>, vol. 108, no. 3, National Academy of Sciences, 2011, pp. E9–10, doi:<a href=\"https://doi.org/10.1073/pnas.1010767108\">10.1073/pnas.1010767108</a>.","ista":"Krens G, Möllmert S, Heisenberg C-PJ. 2011. Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. PNAS. 108(3), E9–E10.","short":"G. Krens, S. Möllmert, C.-P.J. Heisenberg, PNAS 108 (2011) E9–E10."},"type":"journal_article","page":"E9 - E10","day":"18","publisher":"National Academy of Sciences","doi":"10.1073/pnas.1010767108","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T12:02:56Z","intvolume":"       108","quality_controlled":"1","language":[{"iso":"eng"}],"external_id":{"isi":["000286310300003"],"pmid":["21212360"]},"corr_author":"1","date_updated":"2025-09-30T09:02:21Z"},{"publication":"Optics Letters","publist_id":"3234","main_file_link":[{"open_access":"1","url":"https://www.osapublishing.org/ol/abstract.cfm?uri=ol-36-7-1260"}],"volume":36,"citation":{"ama":"Jahnel M, Behrndt M, Jannasch A, Schaeffer E, Grill S. Measuring the complete force field of an optical trap. <i>Optics Letters</i>. 2011;36(7):1260-1262. doi:<a href=\"https://doi.org/10.1364/OL.36.001260\">10.1364/OL.36.001260</a>","chicago":"Jahnel, Marcus, Martin Behrndt, Anita Jannasch, Erik Schaeffer, and Stephan Grill. “Measuring the Complete Force Field of an Optical Trap.” <i>Optics Letters</i>. Optica Publishing Group, 2011. <a href=\"https://doi.org/10.1364/OL.36.001260\">https://doi.org/10.1364/OL.36.001260</a>.","short":"M. Jahnel, M. Behrndt, A. Jannasch, E. Schaeffer, S. Grill, Optics Letters 36 (2011) 1260–1262.","ieee":"M. Jahnel, M. Behrndt, A. Jannasch, E. Schaeffer, and S. Grill, “Measuring the complete force field of an optical trap,” <i>Optics Letters</i>, vol. 36, no. 7. Optica Publishing Group, pp. 1260–1262, 2011.","mla":"Jahnel, Marcus, et al. “Measuring the Complete Force Field of an Optical Trap.” <i>Optics Letters</i>, vol. 36, no. 7, Optica Publishing Group, 2011, pp. 1260–62, doi:<a href=\"https://doi.org/10.1364/OL.36.001260\">10.1364/OL.36.001260</a>.","ista":"Jahnel M, Behrndt M, Jannasch A, Schaeffer E, Grill S. 2011. Measuring the complete force field of an optical trap. Optics Letters. 36(7), 1260–1262.","apa":"Jahnel, M., Behrndt, M., Jannasch, A., Schaeffer, E., &#38; Grill, S. (2011). Measuring the complete force field of an optical trap. <i>Optics Letters</i>. Optica Publishing Group. <a href=\"https://doi.org/10.1364/OL.36.001260\">https://doi.org/10.1364/OL.36.001260</a>"},"related_material":{"record":[{"status":"public","id":"1403","relation":"dissertation_contains"}]},"type":"journal_article","page":"1260 - 1262","doi":"10.1364/OL.36.001260","day":"30","publisher":"Optica Publishing Group","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T12:02:58Z","intvolume":"        36","language":[{"iso":"eng"}],"quality_controlled":"1","external_id":{"isi":["000289251000080"]},"date_updated":"2026-03-09T14:56:18Z","article_processing_charge":"No","month":"03","oa_version":"Published Version","year":"2011","issue":"7","_id":"3373","title":"Measuring the complete force field of an optical trap","author":[{"last_name":"Jahnel","first_name":"Marcus","full_name":"Jahnel, Marcus"},{"last_name":"Behrndt","first_name":"Martin","full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Jannasch, Anita","first_name":"Anita","last_name":"Jannasch"},{"last_name":"Schaeffer","full_name":"Schaeffer, Erik","first_name":"Erik"},{"first_name":"Stephan","full_name":"Grill, Stephan","last_name":"Grill"}],"publication_status":"published","oa":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"status":"public","date_published":"2011-03-30T00:00:00Z","scopus_import":"1","abstract":[{"text":"The use of optical traps to measure or apply forces on the molecular level requires a precise knowledge of the trapping force field. Close to the trap center, this field is typically approximated as linear in the displacement of the trapped microsphere. However, applications demanding high forces at low laser intensities can probe the light-microsphere interaction beyond the linear regime. Here, we measured the full nonlinear force and displacement response of an optical trap in two dimensions using a dual-beam optical trap setup with back-focal-plane photodetection. We observed a substantial stiffening of the trap beyond the linear regime that depends on microsphere size, in agreement with Mie theory calculations. Surprisingly, we found that the linear detection range for forces exceeds the one for displacement by far. Our approach allows for a complete calibration of an optical trap.","lang":"eng"}]},{"volume":354,"citation":{"chicago":"Row, Richard, Jean-Léon Maître, Benjamin Martin, Petra Stockinger, Carl-Philipp J Heisenberg, and David Kimelman. “Completion of the Epithelial to Mesenchymal Transition in Zebrafish Mesoderm Requires Spadetail.” <i>Developmental Biology</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.ydbio.2011.03.025\">https://doi.org/10.1016/j.ydbio.2011.03.025</a>.","ama":"Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. <i>Developmental Biology</i>. 2011;354(1):102-110. doi:<a href=\"https://doi.org/10.1016/j.ydbio.2011.03.025\">10.1016/j.ydbio.2011.03.025</a>","ieee":"R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P. J. Heisenberg, and D. Kimelman, “Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail,” <i>Developmental Biology</i>, vol. 354, no. 1. Elsevier, pp. 102–110, 2011.","apa":"Row, R., Maître, J.-L., Martin, B., Stockinger, P., Heisenberg, C.-P. J., &#38; Kimelman, D. (2011). Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. <i>Developmental Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ydbio.2011.03.025\">https://doi.org/10.1016/j.ydbio.2011.03.025</a>","ista":"Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. 2011. Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. Developmental Biology. 354(1), 102–110.","mla":"Row, Richard, et al. “Completion of the Epithelial to Mesenchymal Transition in Zebrafish Mesoderm Requires Spadetail.” <i>Developmental Biology</i>, vol. 354, no. 1, Elsevier, 2011, pp. 102–10, doi:<a href=\"https://doi.org/10.1016/j.ydbio.2011.03.025\">10.1016/j.ydbio.2011.03.025</a>.","short":"R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P.J. Heisenberg, D. Kimelman, Developmental Biology 354 (2011) 102–110."},"type":"journal_article","publisher":"Elsevier","day":"01","doi":"10.1016/j.ydbio.2011.03.025","page":"102 - 110","publication":"Developmental Biology","publist_id":"3228","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090540/"}],"quality_controlled":"1","language":[{"iso":"eng"}],"intvolume":"       354","external_id":{"isi":["000290550500010"],"pmid":["1463614"]},"date_updated":"2025-09-30T08:51:06Z","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T12:03:00Z","oa_version":"Submitted Version","_id":"3379","issue":"1","year":"2011","title":"Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail","article_processing_charge":"No","month":"06","pmid":1,"status":"public","scopus_import":"1","date_published":"2011-06-01T00:00:00Z","abstract":[{"text":"The process of gastrulation is highly conserved across vertebrates on both the genetic and morphological levels, despite great variety in embryonic shape and speed of development. This mechanism spatially separates the germ layers and establishes the organizational foundation for future development. Mesodermal identity is specified in a superficial layer of cells, the epiblast, where cells maintain an epithelioid morphology. These cells involute to join the deeper hypoblast layer where they adopt a migratory, mesenchymal morphology. Expression of a cascade of related transcription factors orchestrates the parallel genetic transition from primitive to mature mesoderm. Although the early and late stages of this process are increasingly well understood, the transition between them has remained largely mysterious. We present here the first high resolution in vivo observations of the blebby transitional morphology of involuting mesodermal cells in a vertebrate embryo. We further demonstrate that the zebrafish spadetail mutation creates a reversible block in the maturation program, stalling cells in the transition state. This mutation creates an ideal system for dissecting the specific properties of cells undergoing the morphological transition of maturing mesoderm, as we demonstrate with a direct measurement of cell–cell adhesion.","lang":"eng"}],"author":[{"last_name":"Row","first_name":"Richard","full_name":"Row, Richard"},{"last_name":"Maître","orcid":"0000-0002-3688-1474","first_name":"Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon"},{"first_name":"Benjamin","full_name":"Martin, Benjamin","last_name":"Martin"},{"last_name":"Stockinger","full_name":"Stockinger, Petra","id":"261CB030-E90D-11E9-B182-F697D44B663C","first_name":"Petra"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg"},{"last_name":"Kimelman","first_name":"David","full_name":"Kimelman, David"}],"article_type":"original","publication_status":"published","oa":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1},{"type":"journal_article","title":"Invited Lectures ‐ Symposia Area","doi":"10.1111/j.1742-4658.2011.08136.x","publisher":"Wiley-Blackwell","day":"01","page":"24 - 24","oa_version":"None","volume":278,"issue":"S1","_id":"3383","citation":{"chicago":"Heisenberg, Carl-Philipp J. “Invited Lectures ‐ Symposia Area.” <i>FEBS Journal</i>. Wiley-Blackwell, 2011. <a href=\"https://doi.org/10.1111/j.1742-4658.2011.08136.x\">https://doi.org/10.1111/j.1742-4658.2011.08136.x</a>.","ama":"Heisenberg C-PJ. Invited Lectures ‐ Symposia Area. <i>FEBS Journal</i>. 2011;278(S1):24-24. doi:<a href=\"https://doi.org/10.1111/j.1742-4658.2011.08136.x\">10.1111/j.1742-4658.2011.08136.x</a>","ieee":"C.-P. J. Heisenberg, “Invited Lectures ‐ Symposia Area,” <i>FEBS Journal</i>, vol. 278, no. S1. Wiley-Blackwell, pp. 24–24, 2011.","apa":"Heisenberg, C.-P. J. (2011). Invited Lectures ‐ Symposia Area. <i>FEBS Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/j.1742-4658.2011.08136.x\">https://doi.org/10.1111/j.1742-4658.2011.08136.x</a>","mla":"Heisenberg, Carl-Philipp J. “Invited Lectures ‐ Symposia Area.” <i>FEBS Journal</i>, vol. 278, no. S1, Wiley-Blackwell, 2011, pp. 24–24, doi:<a href=\"https://doi.org/10.1111/j.1742-4658.2011.08136.x\">10.1111/j.1742-4658.2011.08136.x</a>.","ista":"Heisenberg C-PJ. 2011. Invited Lectures ‐ Symposia Area. FEBS Journal. 278(S1), 24–24.","short":"C.-P.J. Heisenberg, FEBS Journal 278 (2011) 24–24."},"year":"2011","publist_id":"3224","month":"07","publication":"FEBS Journal","date_updated":"2021-01-12T07:43:06Z","status":"public","language":[{"iso":"eng"}],"date_published":"2011-07-01T00:00:00Z","intvolume":"       278","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"CaHe"}],"author":[{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg"}],"date_created":"2018-12-11T12:03:01Z","publication_status":"published"},{"issue":"21","_id":"3396","year":"2011","oa_version":"Published Version","title":"Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube","article_processing_charge":"No","month":"09","date_published":"2011-09-28T00:00:00Z","abstract":[{"lang":"eng","text":"Facial branchiomotor neurons (FBMNs) in zebrafish and mouse embryonic hindbrain undergo a characteristic tangential migration from rhombomere (r) 4, where they are born, to r6/7. Cohesion among neuroepithelial cells (NCs) has been suggested to function in FBMN migration by inhibiting FBMNs positioned in the basal neuroepithelium such that they move apically between NCs towards the midline of the neuroepithelium instead of tangentially along the basal side of the neuroepithelium towards r6/7. However, direct experimental evaluation of this hypothesis is still lacking. Here, we have used a combination of biophysical cell adhesion measurements and high-resolution time-lapse microscopy to determine the role of NC cohesion in FBMN migration. We show that reducing NC cohesion by interfering with Cadherin 2 (Cdh2) activity results in FBMNs positioned at the basal side of the neuroepithelium moving apically towards the neural tube midline instead of tangentially towards r6/7. In embryos with strongly reduced NC cohesion, ectopic apical FBMN movement frequently results in fusion of the bilateral FBMN clusters over the apical midline of the neural tube. By contrast, reducing cohesion among FBMNs by interfering with Contactin 2 (Cntn2) expression in these cells has little effect on apical FBMN movement, but reduces the fusion of the bilateral FBMN clusters in embryos with strongly diminished NC cohesion. These data provide direct experimental evidence that NC cohesion functions in tangential FBMN migration by restricting their apical movement."}],"scopus_import":"1","status":"public","ddc":["570"],"publication_status":"published","article_type":"original","author":[{"last_name":"Stockinger","first_name":"Petra","full_name":"Stockinger, Petra","id":"261CB030-E90D-11E9-B182-F697D44B663C"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg"},{"id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon","first_name":"Jean-Léon","last_name":"Maître","orcid":"0000-0002-3688-1474"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"file":[{"checksum":"ca12b79e01ef36c1ef1aea31cf7e7139","date_updated":"2020-07-14T12:46:12Z","file_name":"2011_Development_Stockinger.pdf","access_level":"open_access","date_created":"2019-10-07T14:19:42Z","creator":"dernst","content_type":"application/pdf","relation":"main_file","file_size":4672439,"file_id":"6930"}],"oa":1,"citation":{"ieee":"P. Stockinger, C.-P. J. Heisenberg, and J.-L. Maître, “Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube,” <i>Development</i>, vol. 138, no. 21. Company of Biologists, pp. 4673–4683, 2011.","mla":"Stockinger, Petra, et al. “Defective Neuroepithelial Cell Cohesion Affects Tangential Branchiomotor Neuron Migration in the Zebrafish Neural Tube.” <i>Development</i>, vol. 138, no. 21, Company of Biologists, 2011, pp. 4673–83, doi:<a href=\"https://doi.org/10.1242/dev.071233\">10.1242/dev.071233</a>.","apa":"Stockinger, P., Heisenberg, C.-P. J., &#38; Maître, J.-L. (2011). Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.071233\">https://doi.org/10.1242/dev.071233</a>","ista":"Stockinger P, Heisenberg C-PJ, Maître J-L. 2011. Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. Development. 138(21), 4673–4683.","short":"P. Stockinger, C.-P.J. Heisenberg, J.-L. Maître, Development 138 (2011) 4673–4683.","chicago":"Stockinger, Petra, Carl-Philipp J Heisenberg, and Jean-Léon Maître. “Defective Neuroepithelial Cell Cohesion Affects Tangential Branchiomotor Neuron Migration in the Zebrafish Neural Tube.” <i>Development</i>. Company of Biologists, 2011. <a href=\"https://doi.org/10.1242/dev.071233\">https://doi.org/10.1242/dev.071233</a>.","ama":"Stockinger P, Heisenberg C-PJ, Maître J-L. Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. <i>Development</i>. 2011;138(21):4673-4683. doi:<a href=\"https://doi.org/10.1242/dev.071233\">10.1242/dev.071233</a>"},"volume":138,"doi":"10.1242/dev.071233","day":"28","publisher":"Company of Biologists","page":"4673 - 4683","type":"journal_article","publication":"Development","publist_id":"3210","language":[{"iso":"eng"}],"quality_controlled":"1","intvolume":"       138","has_accepted_license":"1","date_updated":"2025-09-30T08:41:19Z","corr_author":"1","external_id":{"isi":["000296060100011"]},"file_date_updated":"2020-07-14T12:46:12Z","date_created":"2018-12-11T12:03:06Z","department":[{"_id":"CaHe"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}]},{"status":"public","abstract":[{"lang":"eng","text":"Recent advances in microscopy techniques and biophysical measurements have provided novel insight into the molecular, cellular and biophysical basis of cell adhesion. However, comparably little is known about a core element of cell–cell adhesion—the energy of adhesion at the cell–cell contact. In this review, we discuss approaches to understand the nature and regulation of adhesion energy, and propose strategies to determine adhesion energy between cells in vitro and in vivo."}],"date_published":"2011-10-01T00:00:00Z","scopus_import":"1","oa":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"author":[{"orcid":"0000-0002-3688-1474","last_name":"Maître","first_name":"Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon"},{"first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}],"publication_status":"published","title":"The role of adhesion energy in controlling cell-cell contacts","oa_version":"Submitted Version","_id":"3397","issue":"5","year":"2011","month":"10","article_processing_charge":"No","corr_author":"1","external_id":{"isi":["000296040800002"]},"date_updated":"2025-09-30T08:42:02Z","quality_controlled":"1","language":[{"iso":"eng"}],"intvolume":"        23","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T12:03:06Z","type":"journal_article","day":"01","publisher":"Elsevier","doi":"10.1016/j.ceb.2011.07.004","page":"508 - 514","volume":23,"citation":{"chicago":"Maître, Jean-Léon, and Carl-Philipp J Heisenberg. “The Role of Adhesion Energy in Controlling Cell-Cell Contacts.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.ceb.2011.07.004\">https://doi.org/10.1016/j.ceb.2011.07.004</a>.","ama":"Maître J-L, Heisenberg C-PJ. The role of adhesion energy in controlling cell-cell contacts. <i>Current Opinion in Cell Biology</i>. 2011;23(5):508-514. doi:<a href=\"https://doi.org/10.1016/j.ceb.2011.07.004\">10.1016/j.ceb.2011.07.004</a>","mla":"Maître, Jean-Léon, and Carl-Philipp J. Heisenberg. “The Role of Adhesion Energy in Controlling Cell-Cell Contacts.” <i>Current Opinion in Cell Biology</i>, vol. 23, no. 5, Elsevier, 2011, pp. 508–14, doi:<a href=\"https://doi.org/10.1016/j.ceb.2011.07.004\">10.1016/j.ceb.2011.07.004</a>.","ista":"Maître J-L, Heisenberg C-PJ. 2011. The role of adhesion energy in controlling cell-cell contacts. Current Opinion in Cell Biology. 23(5), 508–514.","apa":"Maître, J.-L., &#38; Heisenberg, C.-P. J. (2011). The role of adhesion energy in controlling cell-cell contacts. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2011.07.004\">https://doi.org/10.1016/j.ceb.2011.07.004</a>","ieee":"J.-L. Maître and C.-P. J. Heisenberg, “The role of adhesion energy in controlling cell-cell contacts,” <i>Current Opinion in Cell Biology</i>, vol. 23, no. 5. Elsevier, pp. 508–514, 2011.","short":"J.-L. Maître, C.-P.J. Heisenberg, Current Opinion in Cell Biology 23 (2011) 508–514."},"publist_id":"3211","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188705/","open_access":"1"}],"publication":"Current Opinion in Cell Biology"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"publication_status":"published","author":[{"orcid":"0000-0003-4761-5996","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel","first_name":"Gabriel"},{"last_name":"Heisenberg","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"date_published":"2011-01-01T00:00:00Z","abstract":[{"text":"During the development of multicellular organisms, cell fate specification is followed by the sorting of different cell types into distinct domains from where the different tissues and organs are formed. Cell sorting involves both the segregation of a mixed population of cells with different fates and properties into distinct domains, and the active maintenance of their segregated state. Because of its biological importance and apparent resemblance to fluid segregation in physics, cell sorting was extensively studied by both biologists and physicists over the last decades. Different theories were developed that try to explain cell sorting on the basis of the physical properties of the constituent cells. However, only recently the molecular and cellular mechanisms that control the physical properties driving cell sorting, have begun to be unraveled. In this review, we will provide an overview of different cell-sorting processes in development and discuss how these processes can be explained by the different sorting theories, and how these theories in turn can be connected to the molecular and cellular mechanisms driving these processes.","lang":"eng"}],"scopus_import":"1","status":"public","month":"01","alternative_title":["Current Topics in Developmental Biology"],"article_processing_charge":"No","title":"Cell sorting in development","_id":"3791","year":"2011","oa_version":"None","date_created":"2018-12-11T12:05:11Z","department":[{"_id":"CaHe"}],"editor":[{"last_name":"Labouesse","first_name":"Michel","full_name":"Labouesse, Michel"}],"date_updated":"2025-09-30T08:37:44Z","corr_author":"1","external_id":{"isi":["000290454200007"]},"language":[{"iso":"eng"}],"quality_controlled":"1","intvolume":"        95","publist_id":"2436","publication":"Forces and Tension in Development","publisher":"Elsevier","day":"01","doi":"10.1016/B978-0-12-385065-2.00006-2","page":"189 - 213","type":"book_chapter","citation":{"apa":"Krens, G., &#38; Heisenberg, C.-P. J. (2011). Cell sorting in development. In M. Labouesse (Ed.), <i>Forces and Tension in Development</i> (Vol. 95, pp. 189–213). Elsevier. <a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">https://doi.org/10.1016/B978-0-12-385065-2.00006-2</a>","ista":"Krens G, Heisenberg C-PJ. 2011.Cell sorting in development. In: Forces and Tension in Development. Current Topics in Developmental Biology, vol. 95, 189–213.","mla":"Krens, Gabriel, and Carl-Philipp J. Heisenberg. “Cell Sorting in Development.” <i>Forces and Tension in Development</i>, edited by Michel Labouesse, vol. 95, Elsevier, 2011, pp. 189–213, doi:<a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">10.1016/B978-0-12-385065-2.00006-2</a>.","ieee":"G. Krens and C.-P. J. Heisenberg, “Cell sorting in development,” in <i>Forces and Tension in Development</i>, vol. 95, M. Labouesse, Ed. Elsevier, 2011, pp. 189–213.","short":"G. Krens, C.-P.J. Heisenberg, in:, M. Labouesse (Ed.), Forces and Tension in Development, Elsevier, 2011, pp. 189–213.","chicago":"Krens, Gabriel, and Carl-Philipp J Heisenberg. “Cell Sorting in Development.” In <i>Forces and Tension in Development</i>, edited by Michel Labouesse, 95:189–213. Elsevier, 2011. <a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">https://doi.org/10.1016/B978-0-12-385065-2.00006-2</a>.","ama":"Krens G, Heisenberg C-PJ. Cell sorting in development. In: Labouesse M, ed. <i>Forces and Tension in Development</i>. Vol 95. Elsevier; 2011:189-213. doi:<a href=\"https://doi.org/10.1016/B978-0-12-385065-2.00006-2\">10.1016/B978-0-12-385065-2.00006-2</a>"},"volume":95},{"type":"dissertation","title":"Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors","day":"12","publisher":"Institute of Science and Technology Austria","oa_version":"None","citation":{"ama":"Maître J-L. Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors. 2011.","chicago":"Maître, Jean-Léon. “Mechanics of Adhesion and De‐adhesion in Zebrafish Germ Layer Progenitors.” Institute of Science and Technology Austria, 2011.","short":"J.-L. Maître, Mechanics of Adhesion and De‐adhesion in Zebrafish Germ Layer Progenitors, Institute of Science and Technology Austria, 2011.","ista":"Maître J-L. 2011. Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors. Institute of Science and Technology Austria.","apa":"Maître, J.-L. (2011). <i>Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors</i>. Institute of Science and Technology Austria.","mla":"Maître, Jean-Léon. <i>Mechanics of Adhesion and De‐adhesion in Zebrafish Germ Layer Progenitors</i>. Institute of Science and Technology Austria, 2011.","ieee":"J.-L. Maître, “Mechanics of adhesion and de‐adhesion in zebrafish germ layer progenitors,” Institute of Science and Technology Austria, 2011."},"_id":"3273","year":"2011","month":"12","publist_id":"3373","article_processing_charge":"No","alternative_title":["ISTA Thesis"],"degree_awarded":"PhD","corr_author":"1","supervisor":[{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"OA_place":"publisher","date_updated":"2026-04-09T14:36:45Z","publication_identifier":{"issn":["2663-337X"]},"status":"public","language":[{"iso":"eng"}],"date_published":"2011-12-12T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","department":[{"_id":"CaHe"}],"author":[{"last_name":"Maître","orcid":"0000-0002-3688-1474","first_name":"Jean-Léon","full_name":"Maître, Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87"}],"publication_status":"published","date_created":"2018-12-11T12:02:23Z"},{"article_processing_charge":"No","month":"09","oa_version":"None","year":"2010","_id":"3788","issue":"2","title":"Finite-size corrections to scaling behavior in sorted cell aggregates","author":[{"last_name":"Klopper","full_name":"Klopper, Abigail","first_name":"Abigail"},{"full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","first_name":"Gabriel","orcid":"0000-0003-4761-5996","last_name":"Krens"},{"full_name":"Grill, Stephan","first_name":"Stephan","last_name":"Grill"},{"first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}],"publication_status":"published","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","scopus_import":"1","date_published":"2010-09-18T00:00:00Z","abstract":[{"lang":"eng","text":"Cell sorting is a widespread phenomenon pivotal to the early development of multicellular organisms. In vitro cell sorting studies have been instrumental in revealing the cellular properties driving this process. However, these studies have as yet been limited to two-dimensional analysis of three-dimensional cell sorting events. Here we describe a method to record the sorting of primary zebrafish ectoderm and mesoderm germ layer progenitor cells in three dimensions over time, and quantitatively analyze their sorting behavior using an order parameter related to heterotypic interface length. We investigate the cell population size dependence of sorted aggregates and find that the germ layer progenitor cells engulfed in the final configuration display a relationship between total interfacial length and system size according to a simple geometrical argument, subject to a finite-size effect."}],"publication":"The European Physical Journal E: Soft Matter and Biological Physics","publist_id":"2439","volume":33,"citation":{"ieee":"A. Klopper, G. Krens, S. Grill, and C.-P. J. Heisenberg, “Finite-size corrections to scaling behavior in sorted cell aggregates,” <i>The European Physical Journal E: Soft Matter and Biological Physics</i>, vol. 33, no. 2. Springer, pp. 99–103, 2010.","apa":"Klopper, A., Krens, G., Grill, S., &#38; Heisenberg, C.-P. J. (2010). Finite-size corrections to scaling behavior in sorted cell aggregates. <i>The European Physical Journal E: Soft Matter and Biological Physics</i>. Springer. <a href=\"https://doi.org/10.1140/epje/i2010-10642-y\">https://doi.org/10.1140/epje/i2010-10642-y</a>","ista":"Klopper A, Krens G, Grill S, Heisenberg C-PJ. 2010. Finite-size corrections to scaling behavior in sorted cell aggregates. The European Physical Journal E: Soft Matter and Biological Physics. 33(2), 99–103.","mla":"Klopper, Abigail, et al. “Finite-Size Corrections to Scaling Behavior in Sorted Cell Aggregates.” <i>The European Physical Journal E: Soft Matter and Biological Physics</i>, vol. 33, no. 2, Springer, 2010, pp. 99–103, doi:<a href=\"https://doi.org/10.1140/epje/i2010-10642-y\">10.1140/epje/i2010-10642-y</a>.","short":"A. Klopper, G. Krens, S. Grill, C.-P.J. Heisenberg, The European Physical Journal E: Soft Matter and Biological Physics 33 (2010) 99–103.","chicago":"Klopper, Abigail, Gabriel Krens, Stephan Grill, and Carl-Philipp J Heisenberg. “Finite-Size Corrections to Scaling Behavior in Sorted Cell Aggregates.” <i>The European Physical Journal E: Soft Matter and Biological Physics</i>. Springer, 2010. <a href=\"https://doi.org/10.1140/epje/i2010-10642-y\">https://doi.org/10.1140/epje/i2010-10642-y</a>.","ama":"Klopper A, Krens G, Grill S, Heisenberg C-PJ. Finite-size corrections to scaling behavior in sorted cell aggregates. <i>The European Physical Journal E: Soft Matter and Biological Physics</i>. 2010;33(2):99-103. doi:<a href=\"https://doi.org/10.1140/epje/i2010-10642-y\">10.1140/epje/i2010-10642-y</a>"},"type":"journal_article","page":"99 - 103","publisher":"Springer","day":"18","doi":"10.1140/epje/i2010-10642-y","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T12:05:10Z","intvolume":"        33","language":[{"iso":"eng"}],"external_id":{"isi":["000284841700002"]},"corr_author":"1","date_updated":"2025-09-30T09:40:53Z"},{"isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_status":"published","author":[{"full_name":"Quesada-Hernández, Elena","id":"EA35229E-E909-11E9-8DF8-C90C5D5AF86E","first_name":"Elena","last_name":"Quesada-Hernández"},{"first_name":"Luca","full_name":"Caneparo, Luca","last_name":"Caneparo"},{"id":"1FAC36B0-E90A-11E9-9D2F-EF31CE0C9C2F","full_name":"Schneider, Sylvia","first_name":"Sylvia","last_name":"Schneider"},{"last_name":"Winkler","full_name":"Winkler, Sylke","first_name":"Sylke"},{"full_name":"Liebling, Michael","first_name":"Michael","last_name":"Liebling"},{"last_name":"Fraser","full_name":"Fraser, Scott","first_name":"Scott"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}],"acknowledgement":"This work was supported by grants from the Fundacion Caja Madrid to E.Q.H. and the Institute of Science and Technology Austria, the Max-Planck-Society, and the Deutsche Forschungsgemeinschaft to C.P.H.\r\nWe are grateful to Jon Clarke, Andy Oates, and Garrett Greenan for reading earlier versions of this manuscript. We thank J. Peychl, H. Ibarra, and P. Pitrone for excellent assistance and advice in multi-photon microscopy and D. White for assistance during the image-processing steps. We also thank D. Panhans for technical assistance, the whole Heisenberg laboratory for useful comments and discussions, and E. Lehmann, J. Hückmann, and G. Junghans for excellent fish care. ","date_published":"2010-11-09T00:00:00Z","abstract":[{"text":"The development of multicellular organisms is dependent on the tight coordination between tissue growth and morphogenesis. The stereotypical orientation of cell divisions has been proposed to be a fundamental mechanism by which proliferating and growing tissues take shape. However, the actual contribution of stereotypical division orientation (SDO) to tissue morphogenesis is unclear. In zebrafish, cell divisions with stereotypical orientation have been implicated in both body-axis elongation and neural rod formation [1, 2], although there is little direct evidence for a critical function of SDO in either of these processes. Here we show that SDO is required for formation of the neural rod midline during neurulation but dispensable for elongation of the body axis during gastrulation. Our data indicate that SDO during both gastrulation and neurulation is dependent on the noncanonical Wnt receptor Frizzled 7 (Fz7) and that interfering with cell division orientation leads to severe defects in neural rod midline formation but not body-axis elongation. These findings suggest a novel function for Fz7-controlled cell division orientation in neural rod midline formation during neurulation. ","lang":"eng"}],"scopus_import":"1","status":"public","month":"11","article_processing_charge":"No","title":"Stereotypical cell division orientation controls neural rod midline formation in zebrafish","_id":"3789","issue":"21","year":"2010","oa_version":"None","date_created":"2018-12-11T12:05:11Z","department":[{"_id":"CaHe"}],"date_updated":"2025-09-30T09:40:27Z","corr_author":"1","external_id":{"isi":["000284193900032"]},"language":[{"iso":"eng"}],"quality_controlled":"1","intvolume":"        20","publist_id":"2438","publication":"Current Biology","publisher":"Cell Press","doi":"10.1016/j.cub.2010.10.009","day":"09","page":"1966 - 1972","type":"journal_article","citation":{"short":"E. Quesada-Hernández, L. Caneparo, S. Schneider, S. Winkler, M. Liebling, S. Fraser, C.-P.J. Heisenberg, Current Biology 20 (2010) 1966–1972.","apa":"Quesada-Hernández, E., Caneparo, L., Schneider, S., Winkler, S., Liebling, M., Fraser, S., &#38; Heisenberg, C.-P. J. (2010). Stereotypical cell division orientation controls neural rod midline formation in zebrafish. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2010.10.009\">https://doi.org/10.1016/j.cub.2010.10.009</a>","mla":"Quesada-Hernández, Elena, et al. “Stereotypical Cell Division Orientation Controls Neural Rod Midline Formation in Zebrafish.” <i>Current Biology</i>, vol. 20, no. 21, Cell Press, 2010, pp. 1966–72, doi:<a href=\"https://doi.org/10.1016/j.cub.2010.10.009\">10.1016/j.cub.2010.10.009</a>.","ista":"Quesada-Hernández E, Caneparo L, Schneider S, Winkler S, Liebling M, Fraser S, Heisenberg C-PJ. 2010. Stereotypical cell division orientation controls neural rod midline formation in zebrafish. Current Biology. 20(21), 1966–1972.","ieee":"E. Quesada-Hernández <i>et al.</i>, “Stereotypical cell division orientation controls neural rod midline formation in zebrafish,” <i>Current Biology</i>, vol. 20, no. 21. Cell Press, pp. 1966–1972, 2010.","ama":"Quesada-Hernández E, Caneparo L, Schneider S, et al. Stereotypical cell division orientation controls neural rod midline formation in zebrafish. <i>Current Biology</i>. 2010;20(21):1966-1972. doi:<a href=\"https://doi.org/10.1016/j.cub.2010.10.009\">10.1016/j.cub.2010.10.009</a>","chicago":"Quesada-Hernández, Elena, Luca Caneparo, Sylvia Schneider, Sylke Winkler, Michael Liebling, Scott Fraser, and Carl-Philipp J Heisenberg. “Stereotypical Cell Division Orientation Controls Neural Rod Midline Formation in Zebrafish.” <i>Current Biology</i>. Cell Press, 2010. <a href=\"https://doi.org/10.1016/j.cub.2010.10.009\">https://doi.org/10.1016/j.cub.2010.10.009</a>."},"volume":20},{"author":[{"last_name":"Diz Muñoz","full_name":"Diz Muñoz, Alba","first_name":"Alba"},{"first_name":"Michael","full_name":"Krieg, Michael","last_name":"Krieg"},{"full_name":"Bergert, Martin","first_name":"Martin","last_name":"Bergert"},{"last_name":"Ibarlucea Benitez","full_name":"Ibarlucea Benitez, Itziar","first_name":"Itziar"},{"first_name":"Daniel","full_name":"Müller, Daniel","last_name":"Müller"},{"last_name":"Paluch","full_name":"Paluch, Ewa","first_name":"Ewa"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"publication_status":"published","oa":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"file":[{"checksum":"52d18c90ca6b02234cea5e8b399b7f46","date_updated":"2020-07-14T12:46:16Z","file_name":"IST-2015-365-v1+1_journal.pbio.1000544.pdf","content_type":"application/pdf","access_level":"open_access","creator":"system","date_created":"2018-12-12T10:08:24Z","file_id":"4685","file_size":799506,"relation":"main_file"}],"status":"public","date_published":"2010-11-30T00:00:00Z","scopus_import":"1","abstract":[{"text":"Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to the underlying actomyosin cortex has been proposed to be important for cellular processes involving membrane deformation. However, little is known about the actual function of membrane-to-cortex attachment (MCA) in cell protrusion formation and migration, in particular in the context of the developing embryo. Here, we use a multidisciplinary approach to study MCA in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells, which migrate using a combination of different protrusion types, namely, lamellipodia, filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity of molecules linking the cortex to the membrane and measuring resulting changes in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors increases the proportion of cellular blebs and reduces the directionality of cell migration. We propose that MCA is a key parameter controlling the relative proportions of different cell protrusion types in mesendoderm progenitors, and thus is key in controlling directed migration during gastrulation.","lang":"eng"}],"ddc":["576"],"acknowledgement":"We would like to thank A. G. Clark, S. Grill, A. Oates, E. Raz, L. Rohde, and M. Zerial for reading earlier versions of the manuscript. We are grateful to W. Zachariae, Y. Arboleda-Estudillo, S. Schneider, P. Stockinger, D. Panhans, M. Biro, J. C. Olaya, and the BIOTEC/MPI-CBG zebrafish and imaging facilities for help and advice at various stages of this project and to J. Helenius for help with programming. This work was supported by grants from the Boehringer Ingelheim Fonds to MK, the Polish Ministry of Science and Higher Education to E. P., and the Deutsche Forschungsgemeinschaft (HE 3231/6-1 and PA 1590/1-1) to CPH and EP.\r\n","article_processing_charge":"No","month":"11","oa_version":"Published Version","_id":"3790","issue":"11","year":"2010","title":"Control of directed cell migration in vivo by membrane-to-cortex attachment","department":[{"_id":"CaHe"}],"article_number":"e1000544","file_date_updated":"2020-07-14T12:46:16Z","date_created":"2018-12-11T12:05:11Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"language":[{"iso":"eng"}],"quality_controlled":"1","intvolume":"         8","external_id":{"isi":["000284762300016"]},"has_accepted_license":"1","date_updated":"2025-09-30T09:39:58Z","publication":"PLoS Biology","publist_id":"2437","volume":8,"citation":{"ista":"Diz Muñoz A, Krieg M, Bergert M, Ibarlucea Benitez I, Müller D, Paluch E, Heisenberg C-PJ. 2010. Control of directed cell migration in vivo by membrane-to-cortex attachment. PLoS Biology. 8(11), e1000544.","mla":"Diz Muñoz, Alba, et al. “Control of Directed Cell Migration in Vivo by Membrane-to-Cortex Attachment.” <i>PLoS Biology</i>, vol. 8, no. 11, e1000544, Public Library of Science, 2010, doi:<a href=\"https://doi.org/10.1371/journal.pbio.1000544\">10.1371/journal.pbio.1000544</a>.","apa":"Diz Muñoz, A., Krieg, M., Bergert, M., Ibarlucea Benitez, I., Müller, D., Paluch, E., &#38; Heisenberg, C.-P. J. (2010). Control of directed cell migration in vivo by membrane-to-cortex attachment. <i>PLoS Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pbio.1000544\">https://doi.org/10.1371/journal.pbio.1000544</a>","ieee":"A. Diz Muñoz <i>et al.</i>, “Control of directed cell migration in vivo by membrane-to-cortex attachment,” <i>PLoS Biology</i>, vol. 8, no. 11. Public Library of Science, 2010.","short":"A. Diz Muñoz, M. Krieg, M. Bergert, I. Ibarlucea Benitez, D. Müller, E. Paluch, C.-P.J. Heisenberg, PLoS Biology 8 (2010).","chicago":"Diz Muñoz, Alba, Michael Krieg, Martin Bergert, Itziar Ibarlucea Benitez, Daniel Müller, Ewa Paluch, and Carl-Philipp J Heisenberg. “Control of Directed Cell Migration in Vivo by Membrane-to-Cortex Attachment.” <i>PLoS Biology</i>. Public Library of Science, 2010. <a href=\"https://doi.org/10.1371/journal.pbio.1000544\">https://doi.org/10.1371/journal.pbio.1000544</a>.","ama":"Diz Muñoz A, Krieg M, Bergert M, et al. Control of directed cell migration in vivo by membrane-to-cortex attachment. <i>PLoS Biology</i>. 2010;8(11). doi:<a href=\"https://doi.org/10.1371/journal.pbio.1000544\">10.1371/journal.pbio.1000544</a>"},"type":"journal_article","day":"30","publisher":"Public Library of Science","doi":"10.1371/journal.pbio.1000544","pubrep_id":"365"},{"page":"2753 - 2768","doi":"10.1038/emboj.2010.182","day":"18","publisher":"Wiley-Blackwell","type":"journal_article","citation":{"ama":"Papusheva E, Heisenberg C-PJ. Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis. <i>EMBO Journal</i>. 2010;29(16):2753-2768. doi:<a href=\"https://doi.org/10.1038/emboj.2010.182\">10.1038/emboj.2010.182</a>","chicago":"Papusheva, Ekaterina, and Carl-Philipp J Heisenberg. “Spatial Organization of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.” <i>EMBO Journal</i>. Wiley-Blackwell, 2010. <a href=\"https://doi.org/10.1038/emboj.2010.182\">https://doi.org/10.1038/emboj.2010.182</a>.","short":"E. Papusheva, C.-P.J. Heisenberg, EMBO Journal 29 (2010) 2753–2768.","apa":"Papusheva, E., &#38; Heisenberg, C.-P. J. (2010). Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2010.182\">https://doi.org/10.1038/emboj.2010.182</a>","ista":"Papusheva E, Heisenberg C-PJ. 2010. Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis. EMBO Journal. 29(16), 2753–2768.","mla":"Papusheva, Ekaterina, and Carl-Philipp J. Heisenberg. “Spatial Organization of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.” <i>EMBO Journal</i>, vol. 29, no. 16, Wiley-Blackwell, 2010, pp. 2753–68, doi:<a href=\"https://doi.org/10.1038/emboj.2010.182\">10.1038/emboj.2010.182</a>.","ieee":"E. Papusheva and C.-P. J. Heisenberg, “Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis,” <i>EMBO Journal</i>, vol. 29, no. 16. Wiley-Blackwell, pp. 2753–2768, 2010."},"volume":29,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924654/"}],"publist_id":"1962","publication":"EMBO Journal","date_updated":"2025-09-30T09:29:30Z","external_id":{"pmid":["20717145"],"isi":["000281006400009"]},"intvolume":"        29","quality_controlled":"1","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"}],"date_created":"2018-12-11T12:07:17Z","department":[{"_id":"Bio"},{"_id":"CaHe"}],"title":"Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis","year":"2010","_id":"4157","issue":"16","oa_version":"Submitted Version","pmid":1,"month":"08","article_processing_charge":"No","scopus_import":"1","date_published":"2010-08-18T00:00:00Z","abstract":[{"text":"Integrin- and cadherin-mediated adhesion is central for cell and tissue morphogenesis, allowing cells and tissues to change shape without loosing integrity. Studies predominantly in cell culture showed that mechanosensation through adhesion structures is achieved by force-mediated modulation of their molecular composition. The specific molecular composition of adhesion sites in turn determines their signalling activity and dynamic reorganization. Here, we will review how adhesion sites respond to mecanical stimuli, and how spatially and temporally regulated signalling from different adhesion sites controls cell migration and tissue morphogenesis.","lang":"eng"}],"status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"oa":1,"publication_status":"published","author":[{"last_name":"Papusheva","full_name":"Papusheva, Ekaterina","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","first_name":"Ekaterina"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}]}]