---
_id: '7097'
abstract:
- lang: eng
text: Early endosomes, also called sorting endosomes, are known to mature into late
endosomesvia the Rab5-mediated endolysosomal trafficking pathway. Thus, early
endosome existence isthought to be maintained by the continual fusion of transport
vesicles from the plasmamembrane and thetrans-Golgi network (TGN). Here we show
instead that endocytosis isdispensable and post-Golgi vesicle transport is crucial
for the formation of endosomes andthe subsequent endolysosomal traffic regulated
by yeast Rab5 Vps21p. Fittingly, all threeproteins required for endosomal nucleotide
exchange on Vps21p arefirst recruited to theTGN before transport to the endosome, namely the GEF Vps9p
and the epsin-relatedadaptors Ent3/5p. The TGN recruitment of these components
is distinctly controlled, withVps9p appearing to require the Arf1p GTPase, and
the Rab11s, Ypt31p/32p. These resultsprovide a different view of endosome formation
and identify the TGN as a critical location forregulating progress through the
endolysosomal trafficking pathway.
article_number: '419'
article_processing_charge: No
article_type: original
author:
- first_name: Makoto
full_name: Nagano, Makoto
last_name: Nagano
- first_name: Junko Y.
full_name: Toshima, Junko Y.
last_name: Toshima
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Jiro
full_name: Toshima, Jiro
last_name: Toshima
citation:
ama: Nagano M, Toshima JY, Siekhaus DE, Toshima J. Rab5-mediated endosome formation
is regulated at the trans-Golgi network. Communications Biology. 2019;2(1).
doi:10.1038/s42003-019-0670-5
apa: Nagano, M., Toshima, J. Y., Siekhaus, D. E., & Toshima, J. (2019). Rab5-mediated
endosome formation is regulated at the trans-Golgi network. Communications
Biology. Springer Nature. https://doi.org/10.1038/s42003-019-0670-5
chicago: Nagano, Makoto, Junko Y. Toshima, Daria E Siekhaus, and Jiro Toshima. “Rab5-Mediated
Endosome Formation Is Regulated at the Trans-Golgi Network.” Communications
Biology. Springer Nature, 2019. https://doi.org/10.1038/s42003-019-0670-5.
ieee: M. Nagano, J. Y. Toshima, D. E. Siekhaus, and J. Toshima, “Rab5-mediated endosome
formation is regulated at the trans-Golgi network,” Communications Biology,
vol. 2, no. 1. Springer Nature, 2019.
ista: Nagano M, Toshima JY, Siekhaus DE, Toshima J. 2019. Rab5-mediated endosome
formation is regulated at the trans-Golgi network. Communications Biology. 2(1),
419.
mla: Nagano, Makoto, et al. “Rab5-Mediated Endosome Formation Is Regulated at the
Trans-Golgi Network.” Communications Biology, vol. 2, no. 1, 419, Springer
Nature, 2019, doi:10.1038/s42003-019-0670-5.
short: M. Nagano, J.Y. Toshima, D.E. Siekhaus, J. Toshima, Communications Biology
2 (2019).
date_created: 2019-11-25T07:55:01Z
date_published: 2019-11-15T00:00:00Z
date_updated: 2023-08-30T07:27:55Z
day: '15'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.1038/s42003-019-0670-5
external_id:
isi:
- '000496767800005'
file:
- access_level: open_access
checksum: c63c69a264fc8a0e52f2b0d482f3bdae
content_type: application/pdf
creator: dernst
date_created: 2019-11-25T07:58:05Z
date_updated: 2020-07-14T12:47:49Z
file_id: '7098'
file_name: 2019_CommunicBiology_Nagano.pdf
file_size: 2626069
relation: main_file
file_date_updated: 2020-07-14T12:47:49Z
has_accepted_license: '1'
intvolume: ' 2'
isi: 1
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
publication: Communications Biology
publication_identifier:
issn:
- 2399-3642
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Rab5-mediated endosome formation is regulated at the trans-Golgi network
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 2
year: '2019'
...
---
_id: '7180'
abstract:
- lang: eng
text: Arabidopsis PIN2 protein directs transport of the phytohormone auxin from
the root tip into the root elongation zone. Variation in hormone transport, which
depends on a delicate interplay between PIN2 sorting to and from polar plasma
membrane domains, determines root growth. By employing a constitutively degraded
version of PIN2, we identify brassinolides as antagonists of PIN2 endocytosis.
This response does not require de novo protein synthesis, but involves early events
in canonical brassinolide signaling. Brassinolide-controlled adjustments in PIN2
sorting and intracellular distribution governs formation of a lateral PIN2 gradient
in gravistimulated roots, coinciding with adjustments in auxin signaling and directional
root growth. Strikingly, simulations indicate that PIN2 gradient formation is
no prerequisite for root bending but rather dampens asymmetric auxin flow and
signaling. Crosstalk between brassinolide signaling and endocytic PIN2 sorting,
thus, appears essential for determining the rate of gravity-induced root curvature
via attenuation of differential cell elongation.
article_number: '5516'
article_processing_charge: No
article_type: original
author:
- first_name: Katarzyna
full_name: Retzer, Katarzyna
last_name: Retzer
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: Nataliia
full_name: Konstantinova, Nataliia
last_name: Konstantinova
- first_name: Kateřina
full_name: Malínská, Kateřina
last_name: Malínská
- first_name: Johannes
full_name: Leitner, Johannes
last_name: Leitner
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
citation:
ama: Retzer K, Akhmanova M, Konstantinova N, et al. Brassinosteroid signaling delimits
root gravitropism via sorting of the Arabidopsis PIN2 auxin transporter. Nature
Communications. 2019;10. doi:10.1038/s41467-019-13543-1
apa: Retzer, K., Akhmanova, M., Konstantinova, N., Malínská, K., Leitner, J., Petrášek,
J., & Luschnig, C. (2019). Brassinosteroid signaling delimits root gravitropism
via sorting of the Arabidopsis PIN2 auxin transporter. Nature Communications.
Springer Nature. https://doi.org/10.1038/s41467-019-13543-1
chicago: Retzer, Katarzyna, Maria Akhmanova, Nataliia Konstantinova, Kateřina Malínská,
Johannes Leitner, Jan Petrášek, and Christian Luschnig. “Brassinosteroid Signaling
Delimits Root Gravitropism via Sorting of the Arabidopsis PIN2 Auxin Transporter.”
Nature Communications. Springer Nature, 2019. https://doi.org/10.1038/s41467-019-13543-1.
ieee: K. Retzer et al., “Brassinosteroid signaling delimits root gravitropism
via sorting of the Arabidopsis PIN2 auxin transporter,” Nature Communications,
vol. 10. Springer Nature, 2019.
ista: Retzer K, Akhmanova M, Konstantinova N, Malínská K, Leitner J, Petrášek J,
Luschnig C. 2019. Brassinosteroid signaling delimits root gravitropism via sorting
of the Arabidopsis PIN2 auxin transporter. Nature Communications. 10, 5516.
mla: Retzer, Katarzyna, et al. “Brassinosteroid Signaling Delimits Root Gravitropism
via Sorting of the Arabidopsis PIN2 Auxin Transporter.” Nature Communications,
vol. 10, 5516, Springer Nature, 2019, doi:10.1038/s41467-019-13543-1.
short: K. Retzer, M. Akhmanova, N. Konstantinova, K. Malínská, J. Leitner, J. Petrášek,
C. Luschnig, Nature Communications 10 (2019).
date_created: 2019-12-15T23:00:43Z
date_published: 2019-12-01T00:00:00Z
date_updated: 2023-09-06T14:08:21Z
day: '01'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.1038/s41467-019-13543-1
external_id:
isi:
- '000500508100001'
pmid:
- '31797871'
file:
- access_level: open_access
checksum: 77e8720a8e0f3091b98159f85be40893
content_type: application/pdf
creator: dernst
date_created: 2019-12-16T07:37:50Z
date_updated: 2020-07-14T12:47:52Z
file_id: '7184'
file_name: 2019_NatureComm_Retzer.pdf
file_size: 5156533
relation: main_file
file_date_updated: 2020-07-14T12:47:52Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 264CBBAC-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: M02379
name: Modeling epithelial tissue mechanics during cell invasion
publication: Nature Communications
publication_identifier:
eissn:
- '20411723'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Brassinosteroid signaling delimits root gravitropism via sorting of the Arabidopsis
PIN2 auxin transporter
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 10
year: '2019'
...
---
_id: '8'
abstract:
- lang: eng
text: Despite their different origins, Drosophila glia and hemocytes are related
cell populations that provide an immune function. Drosophila hemocytes patrol
the body cavity and act as macrophages outside the nervous system whereas glia
originate from the neuroepithelium and provide the scavenger population of the
nervous system. Drosophila glia are hence the functional orthologs of vertebrate
microglia, even though the latter are cells of immune origin that subsequently
move into the brain during development. Interestingly, the Drosophila immune cells
within (glia) and outside the nervous system (hemocytes) require the same transcription
factor Glide/Gcm for their development. This raises the issue of how do glia specifically
differentiate in the nervous system and hemocytes in the procephalic mesoderm.
The Repo homeodomain transcription factor and pan-glial direct target of Glide/Gcm
is known to ensure glial terminal differentiation. Here we show that Repo also
takes center stage in the process that discriminates between glia and hemocytes.
First, Repo expression is repressed in the hemocyte anlagen by mesoderm-specific
factors. Second, Repo ectopic activation in the procephalic mesoderm is sufficient
to repress the expression of hemocyte-specific genes. Third, the lack of Repo
triggers the expression of hemocyte markers in glia. Thus, a complex network of
tissue-specific cues biases the potential of Glide/Gcm. These data allow us to
revise the concept of fate determinants and help us understand the bases of cell
specification. Both sexes were analyzed.SIGNIFICANCE STATEMENTDistinct cell types
often require the same pioneer transcription factor, raising the issue of how
does one factor trigger different fates. In Drosophila, glia and hemocytes provide
a scavenger activity within and outside the nervous system, respectively. While
they both require the Glide/Gcm transcription factor, glia originate from the
ectoderm, hemocytes from the mesoderm. Here we show that tissue-specific factors
inhibit the gliogenic potential of Glide/Gcm in the mesoderm by repressing the
expression of the homeodomain protein Repo, a major glial-specific target of Glide/Gcm.
Repo expression in turn inhibits the expression of hemocyte-specific genes in
the nervous system. These cell-specific networks secure the establishment of the
glial fate only in the nervous system and allow cell diversification.
acknowledgement: This work was supported by INSERM, CNRS, UDS, Ligue Régionale contre
le Cancer, Hôpital de Strasbourg, Association pour la Recherche sur le Cancer (ARC)
and Agence Nationale de la Recherche (ANR) grants. P.B.C. was funded by the ANR
and by the ARSEP (Fondation pour l'Aide à la Recherche sur la Sclérose en Plaques),
and G.T. by governmental and ARC fellowships. This work was also supported by grants
from the Ataxia UK (2491) and the NC3R (NC/L000199/1) awarded to M.F. The Institut
de Génétique et de Biologie Moléculaire et Cellulaire was also supported by a French
state fund through the ANR labex. D.E.S. was funded by Marie Curie Grant CIG 334077/IRTIM.
We thank B. Altenhein, K. Brückner, M. Crozatier, L. Waltzer, M. Logan, E. Kurant,
R. Reuter, E. Kurucz, J.L Dimarcq, J. Hoffmann, C. Goodman, the DHSB, and the BDSC
for reagents and flies. We also thank all of the laboratory members for comments
on the manuscript; C. Diebold, C. Delaporte, M. Pezze, the fly, and imaging and
antibody facilities for technical assistance; and D. Dembele for help with statistics.
In addition, we thank Alison Brewer for help with Luciferase assays.
article_processing_charge: No
article_type: original
author:
- first_name: Guillaume
full_name: Trébuchet, Guillaume
last_name: Trébuchet
- first_name: Pierre B
full_name: Cattenoz, Pierre B
last_name: Cattenoz
- first_name: János
full_name: Zsámboki, János
last_name: Zsámboki
- first_name: David
full_name: Mazaud, David
last_name: Mazaud
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Manolis
full_name: Fanto, Manolis
last_name: Fanto
- first_name: Angela
full_name: Giangrande, Angela
last_name: Giangrande
citation:
ama: Trébuchet G, Cattenoz PB, Zsámboki J, et al. The Repo homeodomain transcription
factor suppresses hematopoiesis in Drosophila and preserves the glial fate. Journal
of Neuroscience. 2019;39(2):238-255. doi:10.1523/JNEUROSCI.1059-18.2018
apa: Trébuchet, G., Cattenoz, P. B., Zsámboki, J., Mazaud, D., Siekhaus, D. E.,
Fanto, M., & Giangrande, A. (2019). The Repo homeodomain transcription factor
suppresses hematopoiesis in Drosophila and preserves the glial fate. Journal
of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.1059-18.2018
chicago: Trébuchet, Guillaume, Pierre B Cattenoz, János Zsámboki, David Mazaud,
Daria E Siekhaus, Manolis Fanto, and Angela Giangrande. “The Repo Homeodomain
Transcription Factor Suppresses Hematopoiesis in Drosophila and Preserves the
Glial Fate.” Journal of Neuroscience. Society for Neuroscience, 2019. https://doi.org/10.1523/JNEUROSCI.1059-18.2018.
ieee: G. Trébuchet et al., “The Repo homeodomain transcription factor suppresses
hematopoiesis in Drosophila and preserves the glial fate,” Journal of Neuroscience,
vol. 39, no. 2. Society for Neuroscience, pp. 238–255, 2019.
ista: Trébuchet G, Cattenoz PB, Zsámboki J, Mazaud D, Siekhaus DE, Fanto M, Giangrande
A. 2019. The Repo homeodomain transcription factor suppresses hematopoiesis in
Drosophila and preserves the glial fate. Journal of Neuroscience. 39(2), 238–255.
mla: Trébuchet, Guillaume, et al. “The Repo Homeodomain Transcription Factor Suppresses
Hematopoiesis in Drosophila and Preserves the Glial Fate.” Journal of Neuroscience,
vol. 39, no. 2, Society for Neuroscience, 2019, pp. 238–55, doi:10.1523/JNEUROSCI.1059-18.2018.
short: G. Trébuchet, P.B. Cattenoz, J. Zsámboki, D. Mazaud, D.E. Siekhaus, M. Fanto,
A. Giangrande, Journal of Neuroscience 39 (2019) 238–255.
date_created: 2018-12-11T11:44:07Z
date_published: 2019-01-09T00:00:00Z
date_updated: 2023-09-19T10:10:55Z
day: '09'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.1523/JNEUROSCI.1059-18.2018
ec_funded: 1
external_id:
isi:
- '000455189900006'
pmid:
- '30504274'
file:
- access_level: open_access
checksum: 8f6925eb4cd1e8747d8ea25929c68de6
content_type: application/pdf
creator: dernst
date_created: 2020-10-02T09:33:28Z
date_updated: 2020-10-02T09:33:28Z
file_id: '8596'
file_name: 2019_JournNeuroscience_Trebuchet.pdf
file_size: 9455414
relation: main_file
success: 1
file_date_updated: 2020-10-02T09:33:28Z
has_accepted_license: '1'
intvolume: ' 39'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 238-255
pmid: 1
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: Journal of Neuroscience
publication_status: published
publisher: Society for Neuroscience
publist_id: '8048'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The Repo homeodomain transcription factor suppresses hematopoiesis in Drosophila
and preserves the glial fate
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 39
year: '2019'
...
---
_id: '6187'
abstract:
- lang: eng
text: Aberrant display of the truncated core1 O-glycan T-antigen is a common feature
of human cancer cells that correlates with metastasis. Here we show that T-antigen
in Drosophila melanogaster macrophages is involved in their developmentally programmed
tissue invasion. Higher macrophage T-antigen levels require an atypical major
facilitator superfamily (MFS) member that we named Minerva which enables macrophage
dissemination and invasion. We characterize for the first time the T and Tn glycoform
O-glycoproteome of the Drosophila melanogaster embryo, and determine that Minerva
increases the presence of T-antigen on proteins in pathways previously linked
to cancer, most strongly on the sulfhydryl oxidase Qsox1 which we show is required
for macrophage tissue entry. Minerva’s vertebrate ortholog, MFSD1, rescues the
minerva mutant’s migration and T-antigen glycosylation defects. We thus identify
a key conserved regulator that orchestrates O-glycosylation on a protein subset
to activate a program governing migration steps important for both development
and cancer metastasis.
acknowledged_ssus:
- _id: LifeSc
article_number: e41801
article_processing_charge: No
author:
- first_name: Katarina
full_name: Valosková, Katarina
id: 46F146FC-F248-11E8-B48F-1D18A9856A87
last_name: Valosková
- first_name: Julia
full_name: Biebl, Julia
id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
last_name: Biebl
- first_name: Marko
full_name: Roblek, Marko
id: 3047D808-F248-11E8-B48F-1D18A9856A87
last_name: Roblek
orcid: 0000-0001-9588-1389
- first_name: Shamsi
full_name: Emtenani, Shamsi
id: 49D32318-F248-11E8-B48F-1D18A9856A87
last_name: Emtenani
orcid: 0000-0001-6981-6938
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Michaela
full_name: Misova, Michaela
id: 495A3C32-F248-11E8-B48F-1D18A9856A87
last_name: Misova
orcid: 0000-0003-2427-6856
- first_name: Aparna
full_name: Ratheesh, Aparna
id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
last_name: Ratheesh
orcid: 0000-0001-7190-0776
- first_name: Patricia
full_name: Rodrigues, Patricia
id: 2CE4065A-F248-11E8-B48F-1D18A9856A87
last_name: Rodrigues
- first_name: Katerina
full_name: Shkarina, Katerina
last_name: Shkarina
- first_name: Ida Signe Bohse
full_name: Larsen, Ida Signe Bohse
last_name: Larsen
- first_name: Sergey Y
full_name: Vakhrushev, Sergey Y
last_name: Vakhrushev
- first_name: Henrik
full_name: Clausen, Henrik
last_name: Clausen
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Valosková K, Bicher J, Roblek M, et al. A conserved major facilitator superfamily
member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion.
eLife. 2019;8. doi:10.7554/elife.41801
apa: Valosková, K., Bicher, J., Roblek, M., Emtenani, S., György, A., Misova, M.,
… Siekhaus, D. E. (2019). A conserved major facilitator superfamily member orchestrates
a subset of O-glycosylation to aid macrophage tissue invasion. ELife. eLife
Sciences Publications. https://doi.org/10.7554/elife.41801
chicago: Valosková, Katarina, Julia Bicher, Marko Roblek, Shamsi Emtenani, Attila
György, Michaela Misova, Aparna Ratheesh, et al. “A Conserved Major Facilitator
Superfamily Member Orchestrates a Subset of O-Glycosylation to Aid Macrophage
Tissue Invasion.” ELife. eLife Sciences Publications, 2019. https://doi.org/10.7554/elife.41801.
ieee: K. Valosková et al., “A conserved major facilitator superfamily member
orchestrates a subset of O-glycosylation to aid macrophage tissue invasion,” eLife,
vol. 8. eLife Sciences Publications, 2019.
ista: Valosková K, Bicher J, Roblek M, Emtenani S, György A, Misova M, Ratheesh
A, Rodrigues P, Shkarina K, Larsen ISB, Vakhrushev SY, Clausen H, Siekhaus DE.
2019. A conserved major facilitator superfamily member orchestrates a subset of
O-glycosylation to aid macrophage tissue invasion. eLife. 8, e41801.
mla: Valosková, Katarina, et al. “A Conserved Major Facilitator Superfamily Member
Orchestrates a Subset of O-Glycosylation to Aid Macrophage Tissue Invasion.” ELife,
vol. 8, e41801, eLife Sciences Publications, 2019, doi:10.7554/elife.41801.
short: K. Valosková, J. Bicher, M. Roblek, S. Emtenani, A. György, M. Misova, A.
Ratheesh, P. Rodrigues, K. Shkarina, I.S.B. Larsen, S.Y. Vakhrushev, H. Clausen,
D.E. Siekhaus, ELife 8 (2019).
date_created: 2019-03-28T13:37:45Z
date_published: 2019-03-26T00:00:00Z
date_updated: 2024-03-28T23:30:30Z
day: '26'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.7554/elife.41801
ec_funded: 1
external_id:
isi:
- '000462530200001'
file:
- access_level: open_access
checksum: cc0d1a512559d52e7e7cb0e9b9854b40
content_type: application/pdf
creator: dernst
date_created: 2019-03-28T14:00:41Z
date_updated: 2020-07-14T12:47:23Z
file_id: '6188'
file_name: 2019_eLife_Valoskova.pdf
file_size: 4496017
relation: main_file
file_date_updated: 2020-07-14T12:47:23Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
project:
- _id: 253CDE40-B435-11E9-9278-68D0E5697425
grant_number: '24283'
name: Examination of the role of a MFS transporter in the migration of Drosophila
immune cells
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: The role of Drosophila TNF alpha in immune cell invasion
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
- _id: 25388084-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '329540'
name: 'Breaking barriers: Investigating the junctional and mechanobiological changes
underlying the ability of Drosophila immune cells to invade an epithelium'
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/new-gene-potentially-involved-in-metastasis-identified/
record:
- id: '6530'
relation: dissertation_contains
- id: '8983'
relation: dissertation_contains
status: public
- id: '6546'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation
to aid macrophage tissue invasion
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 8
year: '2019'
...
---
_id: '6546'
abstract:
- lang: eng
text: "Invasive migration plays a crucial role not only during development and homeostasis
but also in pathological states, such as tumor metastasis. Drosophila macrophage
migration into the extended germband is an interesting system to study invasive
migration. It carries similarities to immune cell transmigration and cancer cell
invasion, therefore studying this process could also bring new understanding of
invasion in higher organisms. In our work, we uncover a highly conserved member
of the major facilitator family that plays a role in tissue invasion through regulation
of glycosylation on a subgroup of proteins and/or by aiding the precise timing
of DN-Cadherin downregulation. \r\n\r\nAberrant display of the truncated core1
O-glycan T-antigen is a common feature of human cancer cells that correlates with
metastasis. Here we show that T-antigen in Drosophila melanogaster macrophages
is involved in their developmentally programmed tissue invasion. Higher macrophage
T-antigen levels require an atypical major facilitator superfamily (MFS) member
that we named Minerva which enables macrophage dissemination and invasion. We
characterize for the first time the T and Tn glycoform O-glycoproteome of the
Drosophila melanogaster embryo, and determine that Minerva increases the presence
of T-antigen on proteins in pathways previously linked to cancer, most strongly
on the sulfhydryl oxidase Qsox1 which we show is required for macrophage tissue
entry. Minerva’s vertebrate ortholog, MFSD1, rescues the minerva mutant’s migration
and T-antigen glycosylation defects. We thus identify \r\na key conserved regulator
that orchestrates O-glycosylation on a protein subset to activate \r\na program
governing migration steps important for both development and cancer metastasis.
\r\n"
acknowledged_ssus:
- _id: Bio
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Katarina
full_name: Valosková, Katarina
id: 46F146FC-F248-11E8-B48F-1D18A9856A87
last_name: Valosková
citation:
ama: Valosková K. The role of a highly conserved major facilitator superfamily member
in Drosophila embryonic macrophage migration. 2019. doi:10.15479/AT:ISTA:6546
apa: Valosková, K. (2019). The role of a highly conserved major facilitator superfamily
member in Drosophila embryonic macrophage migration. Institute of Science
and Technology Austria. https://doi.org/10.15479/AT:ISTA:6546
chicago: Valosková, Katarina. “The Role of a Highly Conserved Major Facilitator
Superfamily Member in Drosophila Embryonic Macrophage Migration.” Institute of
Science and Technology Austria, 2019. https://doi.org/10.15479/AT:ISTA:6546.
ieee: K. Valosková, “The role of a highly conserved major facilitator superfamily
member in Drosophila embryonic macrophage migration,” Institute of Science and
Technology Austria, 2019.
ista: Valosková K. 2019. The role of a highly conserved major facilitator superfamily
member in Drosophila embryonic macrophage migration. Institute of Science and
Technology Austria.
mla: Valosková, Katarina. The Role of a Highly Conserved Major Facilitator Superfamily
Member in Drosophila Embryonic Macrophage Migration. Institute of Science
and Technology Austria, 2019, doi:10.15479/AT:ISTA:6546.
short: K. Valosková, The Role of a Highly Conserved Major Facilitator Superfamily
Member in Drosophila Embryonic Macrophage Migration, Institute of Science and
Technology Austria, 2019.
date_created: 2019-06-07T12:49:19Z
date_published: 2019-06-07T00:00:00Z
date_updated: 2023-09-19T10:15:54Z
day: '07'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: DaSi
doi: 10.15479/AT:ISTA:6546
file:
- access_level: closed
checksum: 68949c2d96210b45b981a23e9c9cd93c
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: khribikova
date_created: 2019-06-07T13:00:04Z
date_updated: 2020-07-14T12:47:33Z
embargo_to: open_access
file_id: '6549'
file_name: Katarina Valoskova_PhD thesis_final version.docx
file_size: 14110626
relation: source_file
- access_level: open_access
checksum: 555329cd76e196c96f5278c480ee2e6e
content_type: application/pdf
creator: khribikova
date_created: 2019-06-07T13:00:08Z
date_updated: 2021-02-11T11:17:14Z
embargo: 2020-06-07
file_id: '6550'
file_name: Katarina Valoskova_PhD thesis_final version.pdf
file_size: 10054156
relation: main_file
file_date_updated: 2021-02-11T11:17:14Z
has_accepted_license: '1'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: '141'
project:
- _id: 253CDE40-B435-11E9-9278-68D0E5697425
grant_number: '24283'
name: Examination of the role of a MFS transporter in the migration of Drosophila
immune cells
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '6187'
relation: part_of_dissertation
status: public
- id: '544'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
title: The role of a highly conserved major facilitator superfamily member in Drosophila
embryonic macrophage migration
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2019'
...
---
_id: '308'
abstract:
- lang: eng
text: Migrating cells penetrate tissue barriers during development, inflammatory
responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally
confined environments requires changes in the mechanical properties of the surrounding
cells using embryonic Drosophila melanogaster hemocytes, also called macrophages,
as a model. We find that macrophage invasion into the germband through transient
separation of the apposing ectoderm and mesoderm requires cell deformations and
reductions in apical tension in the ectoderm. Interestingly, the genetic pathway
governing these mechanical shifts acts downstream of the only known tumor necrosis
factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald.
Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal
cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated
tight junction protein). We therefore elucidate a distinct molecular pathway that
controls tissue tension and demonstrate the importance of such regulation for
invasive migration in vivo.
acknowledged_ssus:
- _id: SSU
article_processing_charge: No
article_type: original
author:
- first_name: Aparna
full_name: Ratheesh, Aparna
id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
last_name: Ratheesh
orcid: 0000-0001-7190-0776
- first_name: Julia
full_name: Biebl, Julia
id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
last_name: Biebl
- first_name: Michael
full_name: Smutny, Michael
last_name: Smutny
- first_name: Jana
full_name: Veselá, Jana
id: 433253EE-F248-11E8-B48F-1D18A9856A87
last_name: Veselá
- first_name: Ekaterina
full_name: Papusheva, Ekaterina
id: 41DB591E-F248-11E8-B48F-1D18A9856A87
last_name: Papusheva
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Alessandra M
full_name: Casano, Alessandra M
id: 3DBA3F4E-F248-11E8-B48F-1D18A9856A87
last_name: Casano
orcid: 0000-0002-6009-6804
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Ratheesh A, Bicher J, Smutny M, et al. Drosophila TNF modulates tissue tension
in the embryo to facilitate macrophage invasive migration. Developmental Cell.
2018;45(3):331-346. doi:10.1016/j.devcel.2018.04.002
apa: Ratheesh, A., Bicher, J., Smutny, M., Veselá, J., Papusheva, E., Krens, G.,
… Siekhaus, D. E. (2018). Drosophila TNF modulates tissue tension in the embryo
to facilitate macrophage invasive migration. Developmental Cell. Elsevier.
https://doi.org/10.1016/j.devcel.2018.04.002
chicago: Ratheesh, Aparna, Julia Bicher, Michael Smutny, Jana Veselá, Ekaterina
Papusheva, Gabriel Krens, Walter Kaufmann, Attila György, Alessandra M Casano,
and Daria E Siekhaus. “Drosophila TNF Modulates Tissue Tension in the Embryo to
Facilitate Macrophage Invasive Migration.” Developmental Cell. Elsevier,
2018. https://doi.org/10.1016/j.devcel.2018.04.002.
ieee: A. Ratheesh et al., “Drosophila TNF modulates tissue tension in the
embryo to facilitate macrophage invasive migration,” Developmental Cell,
vol. 45, no. 3. Elsevier, pp. 331–346, 2018.
ista: Ratheesh A, Bicher J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W,
György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension
in the embryo to facilitate macrophage invasive migration. Developmental Cell.
45(3), 331–346.
mla: Ratheesh, Aparna, et al. “Drosophila TNF Modulates Tissue Tension in the Embryo
to Facilitate Macrophage Invasive Migration.” Developmental Cell, vol.
45, no. 3, Elsevier, 2018, pp. 331–46, doi:10.1016/j.devcel.2018.04.002.
short: A. Ratheesh, J. Bicher, M. Smutny, J. Veselá, E. Papusheva, G. Krens, W.
Kaufmann, A. György, A.M. Casano, D.E. Siekhaus, Developmental Cell 45 (2018)
331–346.
date_created: 2018-12-11T11:45:44Z
date_published: 2018-05-07T00:00:00Z
date_updated: 2023-09-11T13:22:13Z
day: '07'
department:
- _id: DaSi
- _id: CaHe
- _id: Bio
- _id: EM-Fac
- _id: MiSi
doi: 10.1016/j.devcel.2018.04.002
ec_funded: 1
external_id:
isi:
- '000432461400009'
pmid:
- '29738712'
intvolume: ' 45'
isi: 1
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/j.devcel.2018.04.002
month: '05'
oa: 1
oa_version: Published Version
page: 331 - 346
pmid: 1
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: Developmental Cell
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/cells-change-tension-to-make-tissue-barriers-easier-to-get-through/
scopus_import: '1'
status: public
title: Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage
invasive migration
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 45
year: '2018'
...
---
_id: '620'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis requires the coordinated assembly of various
endocytic proteins and lipids at the plasma membrane. Accumulating evidence demonstrates
a crucial role for phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) in endocytosis,
but specific roles for PtdIns(4)P other than as the biosynthetic precursor of
PtdIns(4,5)P2 have not been clarified. In this study we investigated the role
of PtdIns(4)P or PtdIns(4,5)P2 in receptor-mediated endocytosis through the construction
of temperature-sensitive (ts) mutants for the PI 4-kinases Stt4p and Pik1p and
the PtdIns(4) 5-kinase Mss4p. Quantitative analyses of endocytosis revealed that
both the stt4(ts)pik1(ts) and mss4(ts) mutants have a severe defect in endocytic
internalization. Live-cell imaging of endocytic protein dynamics in stt4(ts)pik1(ts)
and mss4(ts) mutants revealed that PtdIns(4)P is required for the recruitment
of the alpha-factor receptor Ste2p to clathrin-coated pits whereas PtdIns(4,5)P2
is required for membrane internalization. We also found that the localization
to endocytic sites of the ENTH/ANTH domain-bearing clathrin adaptors, Ent1p/Ent2p
and Yap1801p/Yap1802p, is significantly impaired in the stt4(ts)pik1(ts) mutant,
but not in the mss4(ts) mutant. These results suggest distinct roles in successive
steps for PtdIns(4)P and PtdIns(4,5)P2 during receptor-mediated endocytosis.
article_number: jcs207696
article_processing_charge: No
author:
- first_name: Wataru
full_name: Yamamoto, Wataru
last_name: Yamamoto
- first_name: Suguru
full_name: Wada, Suguru
last_name: Wada
- first_name: Makoto
full_name: Nagano, Makoto
last_name: Nagano
- first_name: Kaito
full_name: Aoshima, Kaito
last_name: Aoshima
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Junko
full_name: Toshima, Junko
last_name: Toshima
- first_name: Jiro
full_name: Toshima, Jiro
last_name: Toshima
citation:
ama: Yamamoto W, Wada S, Nagano M, et al. Distinct roles for plasma membrane PtdIns
4 P and PtdIns 4 5 P2 during yeast receptor mediated endocytosis. Journal of
Cell Science. 2018;131(1). doi:10.1242/jcs.207696
apa: Yamamoto, W., Wada, S., Nagano, M., Aoshima, K., Siekhaus, D. E., Toshima,
J., & Toshima, J. (2018). Distinct roles for plasma membrane PtdIns 4 P and
PtdIns 4 5 P2 during yeast receptor mediated endocytosis. Journal of Cell Science.
Company of Biologists. https://doi.org/10.1242/jcs.207696
chicago: Yamamoto, Wataru, Suguru Wada, Makoto Nagano, Kaito Aoshima, Daria E Siekhaus,
Junko Toshima, and Jiro Toshima. “Distinct Roles for Plasma Membrane PtdIns 4
P and PtdIns 4 5 P2 during Yeast Receptor Mediated Endocytosis.” Journal of
Cell Science. Company of Biologists, 2018. https://doi.org/10.1242/jcs.207696.
ieee: W. Yamamoto et al., “Distinct roles for plasma membrane PtdIns 4 P
and PtdIns 4 5 P2 during yeast receptor mediated endocytosis,” Journal of Cell
Science, vol. 131, no. 1. Company of Biologists, 2018.
ista: Yamamoto W, Wada S, Nagano M, Aoshima K, Siekhaus DE, Toshima J, Toshima J.
2018. Distinct roles for plasma membrane PtdIns 4 P and PtdIns 4 5 P2 during yeast
receptor mediated endocytosis. Journal of Cell Science. 131(1), jcs207696.
mla: Yamamoto, Wataru, et al. “Distinct Roles for Plasma Membrane PtdIns 4 P and
PtdIns 4 5 P2 during Yeast Receptor Mediated Endocytosis.” Journal of Cell
Science, vol. 131, no. 1, jcs207696, Company of Biologists, 2018, doi:10.1242/jcs.207696.
short: W. Yamamoto, S. Wada, M. Nagano, K. Aoshima, D.E. Siekhaus, J. Toshima, J.
Toshima, Journal of Cell Science 131 (2018).
date_created: 2018-12-11T11:47:32Z
date_published: 2018-01-04T00:00:00Z
date_updated: 2023-09-11T12:57:13Z
day: '04'
department:
- _id: DaSi
doi: 10.1242/jcs.207696
external_id:
isi:
- '000424786900012'
pmid:
- '29192062'
intvolume: ' 131'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/29192062
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '7184'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distinct roles for plasma membrane PtdIns 4 P and PtdIns 4 5 P2 during yeast
receptor mediated endocytosis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 131
year: '2018'
...
---
_id: '192'
abstract:
- lang: eng
text: The phytohormone auxin is the information carrier in a plethora of developmental
and physiological processes in plants(1). It has been firmly established that
canonical, nuclear auxin signalling acts through regulation of gene transcription(2).
Here, we combined microfluidics, live imaging, genetic engineering and computational
modelling to reanalyse the classical case of root growth inhibition(3) by auxin.
We show that Arabidopsis roots react to addition and removal of auxin by extremely
rapid adaptation of growth rate. This process requires intracellular auxin perception
but not transcriptional reprogramming. The formation of the canonical TIR1/AFB-Aux/IAA
co-receptor complex is required for the growth regulation, hinting to a novel,
non-transcriptional branch of this signalling pathway. Our results challenge the
current understanding of root growth regulation by auxin and suggest another,
presumably non-transcriptional, signalling output of the canonical auxin pathway.
article_processing_charge: No
article_type: original
author:
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Matous
full_name: Glanc, Matous
last_name: Glanc
- first_name: Shinya
full_name: Hagihara, Shinya
last_name: Hagihara
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Naoyuki
full_name: Uchida, Naoyuki
last_name: Uchida
- first_name: Keiko U
full_name: Torii, Keiko U
last_name: Torii
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Fendrych M, Akhmanova M, Merrin J, et al. Rapid and reversible root growth
inhibition by TIR1 auxin signalling. Nature Plants. 2018;4(7):453-459.
doi:10.1038/s41477-018-0190-1
apa: Fendrych, M., Akhmanova, M., Merrin, J., Glanc, M., Hagihara, S., Takahashi,
K., … Friml, J. (2018). Rapid and reversible root growth inhibition by TIR1 auxin
signalling. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-018-0190-1
chicago: Fendrych, Matyas, Maria Akhmanova, Jack Merrin, Matous Glanc, Shinya Hagihara,
Koji Takahashi, Naoyuki Uchida, Keiko U Torii, and Jiří Friml. “Rapid and Reversible
Root Growth Inhibition by TIR1 Auxin Signalling.” Nature Plants. Springer
Nature, 2018. https://doi.org/10.1038/s41477-018-0190-1.
ieee: M. Fendrych et al., “Rapid and reversible root growth inhibition by
TIR1 auxin signalling,” Nature Plants, vol. 4, no. 7. Springer Nature,
pp. 453–459, 2018.
ista: Fendrych M, Akhmanova M, Merrin J, Glanc M, Hagihara S, Takahashi K, Uchida
N, Torii KU, Friml J. 2018. Rapid and reversible root growth inhibition by TIR1
auxin signalling. Nature Plants. 4(7), 453–459.
mla: Fendrych, Matyas, et al. “Rapid and Reversible Root Growth Inhibition by TIR1
Auxin Signalling.” Nature Plants, vol. 4, no. 7, Springer Nature, 2018,
pp. 453–59, doi:10.1038/s41477-018-0190-1.
short: M. Fendrych, M. Akhmanova, J. Merrin, M. Glanc, S. Hagihara, K. Takahashi,
N. Uchida, K.U. Torii, J. Friml, Nature Plants 4 (2018) 453–459.
date_created: 2018-12-11T11:45:07Z
date_published: 2018-06-25T00:00:00Z
date_updated: 2023-09-15T12:11:03Z
day: '25'
department:
- _id: JiFr
- _id: DaSi
- _id: NanoFab
doi: 10.1038/s41477-018-0190-1
external_id:
isi:
- '000443221200017'
pmid:
- '29942048'
intvolume: ' 4'
isi: 1
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/29942048
month: '06'
oa: 1
oa_version: Submitted Version
page: 453 - 459
pmid: 1
publication: Nature Plants
publication_status: published
publisher: Springer Nature
publist_id: '7728'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/new-mechanism-for-the-plant-hormone-auxin-discovered/
scopus_import: '1'
status: public
title: Rapid and reversible root growth inhibition by TIR1 auxin signalling
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '14'
abstract:
- lang: eng
text: The intercellular transport of auxin is driven by PIN-formed (PIN) auxin efflux
carriers. PINs are localized at the plasma membrane (PM) and on constitutively
recycling endomembrane vesicles. Therefore, PINs can mediate auxin transport either
by direct translocation across the PM or by pumping auxin into secretory vesicles
(SVs), leading to its secretory release upon fusion with the PM. Which of these
two mechanisms dominates is a matter of debate. Here, we addressed the issue with
a mathematical modeling approach. We demonstrate that the efficiency of secretory
transport depends on SV size, half-life of PINs on the PM, pH, exocytosis frequency
and PIN density. 3D structured illumination microscopy (SIM) was used to determine
PIN density on the PM. Combining this data with published values of the other
parameters, we show that the transport activity of PINs in SVs would have to be
at least 1000× greater than on the PM in order to produce a comparable macroscopic
auxin transport. If both transport mechanisms operated simultaneously and PINs
were equally active on SVs and PM, the contribution of secretion to the total
auxin flux would be negligible. In conclusion, while secretory vesicle-mediated
transport of auxin is an intriguing and theoretically possible model, it is unlikely
to be a major mechanism of auxin transport inplanta.
acknowledgement: 'European Research Council (ERC): 742985 to Jiri Friml; M.A. was
supported by the Austrian Science Fund (FWF) (M2379-B28); AJ was supported by the
Austria Science Fund (FWF): I03630 to Jiri Friml.'
article_processing_charge: No
article_type: original
author:
- first_name: Sander
full_name: Hille, Sander
last_name: Hille
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. Relative contribution
of PIN-containing secretory vesicles and plasma membrane PINs to the directed
auxin transport: Theoretical estimation. International Journal of Molecular
Sciences. 2018;19(11). doi:10.3390/ijms19113566'
apa: 'Hille, S., Akhmanova, M., Glanc, M., Johnson, A. J., & Friml, J. (2018).
Relative contribution of PIN-containing secretory vesicles and plasma membrane
PINs to the directed auxin transport: Theoretical estimation. International
Journal of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms19113566'
chicago: 'Hille, Sander, Maria Akhmanova, Matous Glanc, Alexander J Johnson, and
Jiří Friml. “Relative Contribution of PIN-Containing Secretory Vesicles and Plasma
Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.” International
Journal of Molecular Sciences. MDPI, 2018. https://doi.org/10.3390/ijms19113566.'
ieee: 'S. Hille, M. Akhmanova, M. Glanc, A. J. Johnson, and J. Friml, “Relative
contribution of PIN-containing secretory vesicles and plasma membrane PINs to
the directed auxin transport: Theoretical estimation,” International Journal
of Molecular Sciences, vol. 19, no. 11. MDPI, 2018.'
ista: 'Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. 2018. Relative contribution
of PIN-containing secretory vesicles and plasma membrane PINs to the directed
auxin transport: Theoretical estimation. International Journal of Molecular Sciences.
19(11).'
mla: 'Hille, Sander, et al. “Relative Contribution of PIN-Containing Secretory Vesicles
and Plasma Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.”
International Journal of Molecular Sciences, vol. 19, no. 11, MDPI, 2018,
doi:10.3390/ijms19113566.'
short: S. Hille, M. Akhmanova, M. Glanc, A.J. Johnson, J. Friml, International Journal
of Molecular Sciences 19 (2018).
date_created: 2018-12-11T11:44:09Z
date_published: 2018-11-12T00:00:00Z
date_updated: 2023-09-18T08:09:32Z
day: '12'
ddc:
- '580'
department:
- _id: DaSi
- _id: JiFr
doi: 10.3390/ijms19113566
ec_funded: 1
external_id:
isi:
- '000451528500282'
file:
- access_level: open_access
checksum: e4b59c2599b0ca26ebf5b8434bcde94a
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T16:04:11Z
date_updated: 2020-07-14T12:44:50Z
file_id: '5719'
file_name: 2018_IJMS_Hille.pdf
file_size: 2200593
relation: main_file
file_date_updated: 2020-07-14T12:44:50Z
has_accepted_license: '1'
intvolume: ' 19'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
publication_status: published
publisher: MDPI
publist_id: '8042'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Relative contribution of PIN-containing secretory vesicles and plasma membrane
PINs to the directed auxin transport: Theoretical estimation'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 19
year: '2018'
...
---
_id: '9'
abstract:
- lang: eng
text: 'Immune cells migrating to the sites of infection navigate through diverse
tissue architectures and switch their migratory mechanisms upon demand. However,
little is known about systemic regulators that could allow the acquisition of
these mechanisms. We performed a genetic screen in Drosophila melanogaster to
identify regulators of germband invasion by embryonic macrophages into the confined
space between the ectoderm and mesoderm. We have found that bZIP circadian transcription
factors (TFs) Kayak (dFos) and Vrille (dNFIL3) have opposite effects on macrophage
germband infiltration: Kayak facilitated and Vrille inhibited it. These TFs are
enriched in the macrophages during migration and genetically interact to control
it. Kayak sets a less coordinated mode of migration of the macrophage group and
increases the probability and length of Levy walks. Intriguingly, the motility
of kayak mutant macrophages was also strongly affected during initial germband
invasion but not along another less confined route. Inhibiting Rho1 signaling
within the tail ectoderm partially rescued the Kayak mutant phenotype, strongly
suggesting that migrating macrophages have to overcome a barrier imposed by the
stiffness of the ectoderm. Also, Kayak appeared to be important for the maintenance
of the round cell shape and the rear edge translocation of the macrophages invading
the germband. Complementary to this, the cortical actin cytoskeleton of Kayak-
deficient macrophages was strongly affected. RNA sequencing revealed the filamin
Cheerio and tetraspanin TM4SF to be downstream of Kayak. Chromatin immunoprecipitation
and immunostaining revealed that the formin Diaphanous is another downstream target
of Kayak. Immunostaining revealed that the formin Diaphanous is another downstream
target of Kayak. Indeed, Cheerio, TM4SF and Diaphanous are required within macrophages
for germband invasion, and expression of constitutively active Diaphanous in macrophages
was able to rescue the kayak mutant phenotype. Moreover, Cher and Diaphanous are
also reduced in the macrophages overexpressing Vrille. We hypothesize that Kayak,
through its targets, increases actin polymerization and cortical tension in macrophages
and thus allows extra force generation necessary for macrophage dissemination
and migration through confined stiff tissues, while Vrille counterbalances it.'
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Vera
full_name: Belyaeva, Vera
id: 47F080FE-F248-11E8-B48F-1D18A9856A87
last_name: Belyaeva
citation:
ama: Belyaeva V. Transcriptional regulation of macrophage migration in the Drosophila
melanogaster embryo . 2018. doi:10.15479/AT:ISTA:th1064
apa: Belyaeva, V. (2018). Transcriptional regulation of macrophage migration
in the Drosophila melanogaster embryo . Institute of Science and Technology
Austria. https://doi.org/10.15479/AT:ISTA:th1064
chicago: Belyaeva, Vera. “Transcriptional Regulation of Macrophage Migration in
the Drosophila Melanogaster Embryo .” Institute of Science and Technology Austria,
2018. https://doi.org/10.15479/AT:ISTA:th1064.
ieee: V. Belyaeva, “Transcriptional regulation of macrophage migration in the Drosophila
melanogaster embryo ,” Institute of Science and Technology Austria, 2018.
ista: Belyaeva V. 2018. Transcriptional regulation of macrophage migration in the
Drosophila melanogaster embryo . Institute of Science and Technology Austria.
mla: Belyaeva, Vera. Transcriptional Regulation of Macrophage Migration in the
Drosophila Melanogaster Embryo . Institute of Science and Technology Austria,
2018, doi:10.15479/AT:ISTA:th1064.
short: V. Belyaeva, Transcriptional Regulation of Macrophage Migration in the Drosophila
Melanogaster Embryo , Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:44:08Z
date_published: 2018-07-01T00:00:00Z
date_updated: 2023-09-07T12:43:10Z
day: '01'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: DaSi
doi: 10.15479/AT:ISTA:th1064
file:
- access_level: closed
checksum: d27b2465cb70d0c9678a0381b9b6ced1
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-08T14:13:12Z
date_updated: 2020-07-14T12:48:14Z
embargo_to: open_access
file_id: '6243'
file_name: 2018_Thesis_Belyaeva_source.docx
file_size: 102737483
relation: source_file
- access_level: open_access
checksum: a2939b61bde2de7b8ced77bbae0eaaed
content_type: application/pdf
creator: dernst
date_created: 2019-04-08T14:14:08Z
date_updated: 2021-02-11T11:17:16Z
embargo: 2019-11-19
file_id: '6244'
file_name: 2018_Thesis_Belyaeva.pdf
file_size: 88077843
relation: main_file
file_date_updated: 2021-02-11T11:17:16Z
has_accepted_license: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: '96'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '8047'
pubrep_id: '1064'
status: public
supervisor:
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
title: 'Transcriptional regulation of macrophage migration in the Drosophila melanogaster
embryo '
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '544'
abstract:
- lang: eng
text: Drosophila melanogaster plasmatocytes, the phagocytic cells among hemocytes,
are essential for immune responses, but also play key roles from early development
to death through their interactions with other cell types. They regulate homeostasis
and signaling during development, stem cell proliferation, metabolism, cancer,
wound responses and aging, displaying intriguing molecular and functional conservation
with vertebrate macrophages. Given the relative ease of genetics in Drosophila
compared to vertebrates, tools permitting visualization and genetic manipulation
of plasmatocytes and surrounding tissues independently at all stages would greatly
aid in fully understanding these processes, but are lacking. Here we describe
a comprehensive set of transgenic lines that allow this. These include extremely
brightly fluorescing mCherry-based lines that allow GAL4-independent visualization
of plasmatocyte nuclei, cytoplasm or actin cytoskeleton from embryonic Stage 8
through adulthood in both live and fixed samples even as heterozygotes, greatly
facilitating screening. These lines allow live visualization and tracking of embryonic
plasmatocytes, as well as larval plasmatocytes residing at the body wall or flowing
with the surrounding hemolymph. With confocal imaging, interactions of plasmatocytes
and inner tissues can be seen in live or fixed embryos, larvae and adults. They
permit efficient GAL4-independent FACS analysis/sorting of plasmatocytes throughout
life. To facilitate genetic analysis of reciprocal signaling, we have also made
a plasmatocyte-expressing QF2 line that in combination with extant GAL4 drivers
allows independent genetic manipulation of both plasmatocytes and surrounding
tissues, and a GAL80 line that blocks GAL4 drivers from affecting plasmatocytes,
both of which function from the early embryo to the adult.
acknowledged_ssus:
- _id: LifeSc
acknowledgement: ' A. Ratheesh also by Marie Curie IIF GA-2012-32950BB:DICJI, Marko
Roblek by the provincial government of Lower Austria, K. Valoskova and S. Wachner
by DOC Fellowships from the Austrian Academy of Sciences, '
article_processing_charge: No
author:
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Marko
full_name: Roblek, Marko
id: 3047D808-F248-11E8-B48F-1D18A9856A87
last_name: Roblek
orcid: 0000-0001-9588-1389
- first_name: Aparna
full_name: Ratheesh, Aparna
id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
last_name: Ratheesh
orcid: 0000-0001-7190-0776
- first_name: Katarina
full_name: Valosková, Katarina
id: 46F146FC-F248-11E8-B48F-1D18A9856A87
last_name: Valosková
- first_name: Vera
full_name: Belyaeva, Vera
id: 47F080FE-F248-11E8-B48F-1D18A9856A87
last_name: Belyaeva
- first_name: Stephanie
full_name: Wachner, Stephanie
id: 2A95E7B0-F248-11E8-B48F-1D18A9856A87
last_name: Wachner
- first_name: Yutaka
full_name: Matsubayashi, Yutaka
last_name: Matsubayashi
- first_name: Besaiz
full_name: Sanchez Sanchez, Besaiz
last_name: Sanchez Sanchez
- first_name: Brian
full_name: Stramer, Brian
last_name: Stramer
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: 'György A, Roblek M, Ratheesh A, et al. Tools allowing independent visualization
and genetic manipulation of Drosophila melanogaster macrophages and surrounding
tissues. G3: Genes, Genomes, Genetics. 2018;8(3):845-857. doi:10.1534/g3.117.300452'
apa: 'György, A., Roblek, M., Ratheesh, A., Valosková, K., Belyaeva, V., Wachner,
S., … Siekhaus, D. E. (2018). Tools allowing independent visualization and genetic
manipulation of Drosophila melanogaster macrophages and surrounding tissues. G3:
Genes, Genomes, Genetics. Genetics Society of America. https://doi.org/10.1534/g3.117.300452'
chicago: 'György, Attila, Marko Roblek, Aparna Ratheesh, Katarina Valosková, Vera
Belyaeva, Stephanie Wachner, Yutaka Matsubayashi, Besaiz Sanchez Sanchez, Brian
Stramer, and Daria E Siekhaus. “Tools Allowing Independent Visualization and Genetic
Manipulation of Drosophila Melanogaster Macrophages and Surrounding Tissues.”
G3: Genes, Genomes, Genetics. Genetics Society of America, 2018. https://doi.org/10.1534/g3.117.300452.'
ieee: 'A. György et al., “Tools allowing independent visualization and genetic
manipulation of Drosophila melanogaster macrophages and surrounding tissues,”
G3: Genes, Genomes, Genetics, vol. 8, no. 3. Genetics Society of America,
pp. 845–857, 2018.'
ista: 'György A, Roblek M, Ratheesh A, Valosková K, Belyaeva V, Wachner S, Matsubayashi
Y, Sanchez Sanchez B, Stramer B, Siekhaus DE. 2018. Tools allowing independent
visualization and genetic manipulation of Drosophila melanogaster macrophages
and surrounding tissues. G3: Genes, Genomes, Genetics. 8(3), 845–857.'
mla: 'György, Attila, et al. “Tools Allowing Independent Visualization and Genetic
Manipulation of Drosophila Melanogaster Macrophages and Surrounding Tissues.”
G3: Genes, Genomes, Genetics, vol. 8, no. 3, Genetics Society of America,
2018, pp. 845–57, doi:10.1534/g3.117.300452.'
short: 'A. György, M. Roblek, A. Ratheesh, K. Valosková, V. Belyaeva, S. Wachner,
Y. Matsubayashi, B. Sanchez Sanchez, B. Stramer, D.E. Siekhaus, G3: Genes, Genomes,
Genetics 8 (2018) 845–857.'
date_created: 2018-12-11T11:47:05Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2024-03-28T23:30:30Z
day: '01'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.1534/g3.117.300452
ec_funded: 1
external_id:
isi:
- '000426693300011'
file:
- access_level: open_access
checksum: 7d9d28b915159078a4ca7add568010e8
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:48Z
date_updated: 2020-07-14T12:46:56Z
file_id: '4905'
file_name: IST-2018-990-v1+1_2018_Gyoergy_Tools_allowing.pdf
file_size: 2251222
relation: main_file
file_date_updated: 2020-07-14T12:46:56Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 845 - 857
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: The role of Drosophila TNF alpha in immune cell invasion
- _id: 2637E9C0-B435-11E9-9278-68D0E5697425
grant_number: 'LSC16-021 '
name: Investigating the role of the novel major superfamily facilitator transporter
family member MFSD1 in metastasis
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: 'G3: Genes, Genomes, Genetics'
publication_status: published
publisher: Genetics Society of America
publist_id: '7271'
pubrep_id: '990'
quality_controlled: '1'
related_material:
record:
- id: '6530'
relation: research_paper
- id: '6543'
relation: research_paper
- id: '11193'
relation: dissertation_contains
status: public
- id: '6546'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Tools allowing independent visualization and genetic manipulation of Drosophila
melanogaster macrophages and surrounding tissues
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2018'
...
---
_id: '751'
abstract:
- lang: eng
text: The basement membrane (BM) is a thin layer of extracellular matrix (ECM) beneath
nearly all epithelial cell types that is critical for cellular and tissue function.
It is composed of numerous components conserved among all bilaterians [1]; however,
it is unknown how all of these components are generated and subsequently constructed
to form a fully mature BM in the living animal. Although BM formation is thought
to simply involve a process of self-assembly [2], this concept suffers from a
number of logistical issues when considering its construction in vivo. First,
incorporation of BM components appears to be hierarchical [3-5], yet it is unclear
whether their production during embryogenesis must also be regulated in a temporal
fashion. Second, many BM proteins are produced not only by the cells residing
on the BM but also by surrounding cell types [6-9], and it is unclear how large,
possibly insoluble protein complexes [10] are delivered into the matrix. Here
we exploit our ability to live image and genetically dissect de novo BM formation
during Drosophila development. This reveals that there is a temporal hierarchy
of BM protein production that is essential for proper component incorporation.
Furthermore, we show that BM components require secretion by migrating macrophages
(hemocytes) during their developmental dispersal, which is critical for embryogenesis.
Indeed, hemocyte migration is essential to deliver a subset of ECM components
evenly throughout the embryo. This reveals that de novo BM construction requires
a combination of both production and distribution logistics allowing for the timely
delivery of core components.
article_processing_charge: No
author:
- first_name: Yutaka
full_name: Matsubayashi, Yutaka
last_name: Matsubayashi
- first_name: Adam
full_name: Louani, Adam
last_name: Louani
- first_name: Anca
full_name: Dragu, Anca
last_name: Dragu
- first_name: Besaiz
full_name: Sanchez Sanchez, Besaiz
last_name: Sanchez Sanchez
- first_name: Eduardo
full_name: Serna Morales, Eduardo
last_name: Serna Morales
- first_name: Lawrence
full_name: Yolland, Lawrence
last_name: Yolland
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Gema
full_name: Vizcay, Gema
last_name: Vizcay
- first_name: Roland
full_name: Fleck, Roland
last_name: Fleck
- first_name: John
full_name: Heddleston, John
last_name: Heddleston
- first_name: Teng
full_name: Chew, Teng
last_name: Chew
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Brian
full_name: Stramer, Brian
last_name: Stramer
citation:
ama: Matsubayashi Y, Louani A, Dragu A, et al. A moving source of matrix components
is essential for De Novo basement membrane formation. Current Biology.
2017;27(22):3526-3534e.4. doi:10.1016/j.cub.2017.10.001
apa: Matsubayashi, Y., Louani, A., Dragu, A., Sanchez Sanchez, B., Serna Morales,
E., Yolland, L., … Stramer, B. (2017). A moving source of matrix components is
essential for De Novo basement membrane formation. Current Biology. Cell
Press. https://doi.org/10.1016/j.cub.2017.10.001
chicago: Matsubayashi, Yutaka, Adam Louani, Anca Dragu, Besaiz Sanchez Sanchez,
Eduardo Serna Morales, Lawrence Yolland, Attila György, et al. “A Moving Source
of Matrix Components Is Essential for De Novo Basement Membrane Formation.” Current
Biology. Cell Press, 2017. https://doi.org/10.1016/j.cub.2017.10.001.
ieee: Y. Matsubayashi et al., “A moving source of matrix components is essential
for De Novo basement membrane formation,” Current Biology, vol. 27, no.
22. Cell Press, p. 3526–3534e.4, 2017.
ista: Matsubayashi Y, Louani A, Dragu A, Sanchez Sanchez B, Serna Morales E, Yolland
L, György A, Vizcay G, Fleck R, Heddleston J, Chew T, Siekhaus DE, Stramer B.
2017. A moving source of matrix components is essential for De Novo basement membrane
formation. Current Biology. 27(22), 3526–3534e.4.
mla: Matsubayashi, Yutaka, et al. “A Moving Source of Matrix Components Is Essential
for De Novo Basement Membrane Formation.” Current Biology, vol. 27, no.
22, Cell Press, 2017, p. 3526–3534e.4, doi:10.1016/j.cub.2017.10.001.
short: Y. Matsubayashi, A. Louani, A. Dragu, B. Sanchez Sanchez, E. Serna Morales,
L. Yolland, A. György, G. Vizcay, R. Fleck, J. Heddleston, T. Chew, D.E. Siekhaus,
B. Stramer, Current Biology 27 (2017) 3526–3534e.4.
date_created: 2018-12-11T11:48:18Z
date_published: 2017-11-09T00:00:00Z
date_updated: 2023-09-27T12:25:31Z
day: '09'
ddc:
- '570'
- '576'
department:
- _id: DaSi
doi: 10.1016/j.cub.2017.10.001
external_id:
isi:
- '000415815800031'
file:
- access_level: open_access
checksum: 264cf6c6c3551486ba5ea786850e000a
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:45Z
date_updated: 2020-07-14T12:47:59Z
file_id: '4770'
file_name: IST-2017-875-v1+1_1-s2.0-S0960982217312691-main.pdf
file_size: 4770657
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 27'
isi: 1
issue: '22'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 3526 - 3534e.4
publication: Current Biology
publication_identifier:
issn:
- '09609822'
publication_status: published
publisher: Cell Press
publist_id: '6905'
pubrep_id: '875'
quality_controlled: '1'
scopus_import: '1'
status: public
title: A moving source of matrix components is essential for De Novo basement membrane
formation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 27
year: '2017'
...
---
_id: '1476'
abstract:
- lang: eng
text: The dynamic assembly and disassembly of actin filaments is essential for the
formation and transport of vesicles during endocytosis. In yeast, two types of
actin structures, namely cortical patches and cytoplasmic cables, play a direct
role in endocytosis, but how their interaction is regulated remains unclear. Here,
we show that Srv2/CAP, an evolutionarily conserved actin regulator, is required
for efficient endocytosis owing to its role in the formation of the actin patches
that aid initial vesicle invagination and of the actin cables that these move
along. Deletion of the SRV2 gene resulted in the appearance of aberrant fragmented
actin cables that frequently moved past actin patches, the sites of endocytosis.
We find that the C-terminal CARP domain of Srv2p is vitally important for the
proper assembly of actin patches and cables; we also demonstrate that the N-terminal
helical folded domain of Srv2 is required for its localization to actin patches,
specifically to the ADP-actin rich region through an interaction with cofilin.
These results demonstrate the in vivo roles of Srv2p in the regulation of the
actin cytoskeleton during clathrin-mediated endocytosis
acknowledgement: We are grateful to Anthony Bretscher (Cornell University, NY) for
providing the bni1-12 bnr1Δ (Y4135) strain. J.Y.T. was supported by a Japan Society
for the Promotion of Science (JSPS) KAKENHI grant [grant number 26440067]; the Takeda
Science Foundation; and the Novartis Foundation (Japan). J.T. was supported by a
JSPS KAKENHI grant [grant number 25440054]; the Takeda Science Foundation; and the
Kurata Memorial Hitachi Science and Technology Foundation. D.E.S. was supported
by the European Union [grant number PCIG12-GA-2012-334077].
author:
- first_name: Junko
full_name: Toshima, Junko
last_name: Toshima
- first_name: Chika
full_name: Horikomi, Chika
last_name: Horikomi
- first_name: Asuka
full_name: Okada, Asuka
last_name: Okada
- first_name: Makiko
full_name: Hatori, Makiko
last_name: Hatori
- first_name: Makoto
full_name: Nagano, Makoto
last_name: Nagano
- first_name: Atsushi
full_name: Masuda, Atsushi
last_name: Masuda
- first_name: Wataru
full_name: Yamamoto, Wataru
last_name: Yamamoto
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Jiro
full_name: Toshima, Jiro
last_name: Toshima
citation:
ama: Toshima J, Horikomi C, Okada A, et al. Srv2/CAP is required for polarized actin
cable assembly and patch internalization during clathrin-mediated endocytosis.
Journal of Cell Science. 2016;129(2):367-379. doi:10.1242/jcs.176651
apa: Toshima, J., Horikomi, C., Okada, A., Hatori, M., Nagano, M., Masuda, A., …
Toshima, J. (2016). Srv2/CAP is required for polarized actin cable assembly and
patch internalization during clathrin-mediated endocytosis. Journal of Cell
Science. Company of Biologists. https://doi.org/10.1242/jcs.176651
chicago: Toshima, Junko, Chika Horikomi, Asuka Okada, Makiko Hatori, Makoto Nagano,
Atsushi Masuda, Wataru Yamamoto, Daria E Siekhaus, and Jiro Toshima. “Srv2/CAP
Is Required for Polarized Actin Cable Assembly and Patch Internalization during
Clathrin-Mediated Endocytosis.” Journal of Cell Science. Company of Biologists,
2016. https://doi.org/10.1242/jcs.176651.
ieee: J. Toshima et al., “Srv2/CAP is required for polarized actin cable
assembly and patch internalization during clathrin-mediated endocytosis,” Journal
of Cell Science, vol. 129, no. 2. Company of Biologists, pp. 367–379, 2016.
ista: Toshima J, Horikomi C, Okada A, Hatori M, Nagano M, Masuda A, Yamamoto W,
Siekhaus DE, Toshima J. 2016. Srv2/CAP is required for polarized actin cable assembly
and patch internalization during clathrin-mediated endocytosis. Journal of Cell
Science. 129(2), 367–379.
mla: Toshima, Junko, et al. “Srv2/CAP Is Required for Polarized Actin Cable Assembly
and Patch Internalization during Clathrin-Mediated Endocytosis.” Journal of
Cell Science, vol. 129, no. 2, Company of Biologists, 2016, pp. 367–79, doi:10.1242/jcs.176651.
short: J. Toshima, C. Horikomi, A. Okada, M. Hatori, M. Nagano, A. Masuda, W. Yamamoto,
D.E. Siekhaus, J. Toshima, Journal of Cell Science 129 (2016) 367–379.
date_created: 2018-12-11T11:52:14Z
date_published: 2016-01-15T00:00:00Z
date_updated: 2021-01-12T06:51:00Z
day: '15'
ddc:
- '570'
- '576'
department:
- _id: DaSi
doi: 10.1242/jcs.176651
ec_funded: 1
file:
- access_level: open_access
checksum: 2da0a09149a9ed956cdf79a95c17f08a
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:08Z
date_updated: 2020-07-14T12:44:56Z
file_id: '4861'
file_name: IST-2017-767-v1+1_367.full.pdf
file_size: 7176912
relation: main_file
file_date_updated: 2020-07-14T12:44:56Z
has_accepted_license: '1'
intvolume: ' 129'
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 367 - 379
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '5720'
pubrep_id: '767'
quality_controlled: '1'
scopus_import: 1
status: public
title: Srv2/CAP is required for polarized actin cable assembly and patch internalization
during clathrin-mediated endocytosis
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 129
year: '2016'
...
---
_id: '1475'
abstract:
- lang: eng
text: The actin cytoskeleton plays important roles in the formation and internalization
of endocytic vesicles. In yeast, endocytic vesicles move towards early endosomes
along actin cables, however, the molecular machinery regulating interaction between
endocytic vesicles and actin cables is poorly understood. The Eps15-like protein
Pan1p plays a key role in actin-mediated endocytosis and is negatively regulated
by Ark1 and Prk1 kinases. Here we show that pan1 mutated to prevent phosphorylation
at all 18 threonines, pan1-18TA, displayed almost the same endocytic defect as
ark1Δ prk1Δ cells, and contained abnormal actin concentrations including several
endocytic compartments. Early endosomes were highly localized in the actin concentrations
and displayed movement along actin cables. The dephosphorylated form of Pan1p
also caused stable associations between endocytic vesicles and actin cables, and
between endocytic vesicles and endosomes. Thus Pan1 phosphorylation is part of
a novel mechanism that regulates endocytic compartment interactions with each
other and with actin cables.
article_number: e10276
author:
- first_name: Junko
full_name: Toshima, Junko
last_name: Toshima
- first_name: Eri
full_name: Furuya, Eri
last_name: Furuya
- first_name: Makoto
full_name: Nagano, Makoto
last_name: Nagano
- first_name: Chisa
full_name: Kanno, Chisa
last_name: Kanno
- first_name: Yuta
full_name: Sakamoto, Yuta
last_name: Sakamoto
- first_name: Masashi
full_name: Ebihara, Masashi
last_name: Ebihara
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Jiro
full_name: Toshima, Jiro
last_name: Toshima
citation:
ama: Toshima J, Furuya E, Nagano M, et al. Yeast Eps15-like endocytic protein Pan1p
regulates the interaction between endocytic vesicles, endosomes and the actin
cytoskeleton. eLife. 2016;5(February 2016). doi:10.7554/eLife.10276
apa: Toshima, J., Furuya, E., Nagano, M., Kanno, C., Sakamoto, Y., Ebihara, M.,
… Toshima, J. (2016). Yeast Eps15-like endocytic protein Pan1p regulates the interaction
between endocytic vesicles, endosomes and the actin cytoskeleton. ELife.
eLife Sciences Publications. https://doi.org/10.7554/eLife.10276
chicago: Toshima, Junko, Eri Furuya, Makoto Nagano, Chisa Kanno, Yuta Sakamoto,
Masashi Ebihara, Daria E Siekhaus, and Jiro Toshima. “Yeast Eps15-like Endocytic
Protein Pan1p Regulates the Interaction between Endocytic Vesicles, Endosomes
and the Actin Cytoskeleton.” ELife. eLife Sciences Publications, 2016.
https://doi.org/10.7554/eLife.10276.
ieee: J. Toshima et al., “Yeast Eps15-like endocytic protein Pan1p regulates
the interaction between endocytic vesicles, endosomes and the actin cytoskeleton,”
eLife, vol. 5, no. February 2016. eLife Sciences Publications, 2016.
ista: Toshima J, Furuya E, Nagano M, Kanno C, Sakamoto Y, Ebihara M, Siekhaus DE,
Toshima J. 2016. Yeast Eps15-like endocytic protein Pan1p regulates the interaction
between endocytic vesicles, endosomes and the actin cytoskeleton. eLife. 5(February
2016), e10276.
mla: Toshima, Junko, et al. “Yeast Eps15-like Endocytic Protein Pan1p Regulates
the Interaction between Endocytic Vesicles, Endosomes and the Actin Cytoskeleton.”
ELife, vol. 5, no. February 2016, e10276, eLife Sciences Publications,
2016, doi:10.7554/eLife.10276.
short: J. Toshima, E. Furuya, M. Nagano, C. Kanno, Y. Sakamoto, M. Ebihara, D.E.
Siekhaus, J. Toshima, ELife 5 (2016).
date_created: 2018-12-11T11:52:14Z
date_published: 2016-02-25T00:00:00Z
date_updated: 2021-01-12T06:50:59Z
day: '25'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.7554/eLife.10276
ec_funded: 1
file:
- access_level: open_access
checksum: d1cc44870580756ba8badd8e41adfdb5
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:10:08Z
date_updated: 2020-07-14T12:44:56Z
file_id: '4793'
file_name: IST-2016-529-v1+1_elife-10276-v1.pdf
file_size: 5198001
relation: main_file
file_date_updated: 2020-07-14T12:44:56Z
has_accepted_license: '1'
intvolume: ' 5'
issue: February 2016
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '5721'
pubrep_id: '529'
quality_controlled: '1'
scopus_import: 1
status: public
title: Yeast Eps15-like endocytic protein Pan1p regulates the interaction between
endocytic vesicles, endosomes and the actin cytoskeleton
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2016'
...
---
_id: '1712'
abstract:
- lang: eng
text: The majority of immune cells in Drosophila melanogaster are plasmatocytes;
they carry out similar functions to vertebrate macrophages, influencing development
as well as protecting against infection and cancer. Plasmatocytes, sometimes referred
to with the broader term of hemocytes, migrate widely during embryonic development
and cycle in the larvae between sessile and circulating positions. Here we discuss
the similarities of plasmatocyte developmental migration and its functions to
that of vertebrate macrophages, considering the recent controversy regarding the
functions of Drosophila PDGF/VEGF related ligands. We also examine recent findings
on the significance of adhesion for plasmatocyte migration in the embryo, as well
as proliferation, trans-differentiation, and tumor responses in the larva. We
spotlight parallels throughout to vertebrate immune responses.
author:
- first_name: Aparna
full_name: Ratheesh, Aparna
id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
last_name: Ratheesh
- first_name: Vera
full_name: Belyaeva, Vera
id: 47F080FE-F248-11E8-B48F-1D18A9856A87
last_name: Belyaeva
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Ratheesh A, Belyaeva V, Siekhaus DE. Drosophila immune cell migration and adhesion
during embryonic development and larval immune responses. Current Opinion in
Cell Biology. 2015;36(10):71-79. doi:10.1016/j.ceb.2015.07.003
apa: Ratheesh, A., Belyaeva, V., & Siekhaus, D. E. (2015). Drosophila immune
cell migration and adhesion during embryonic development and larval immune responses.
Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2015.07.003
chicago: Ratheesh, Aparna, Vera Belyaeva, and Daria E Siekhaus. “Drosophila Immune
Cell Migration and Adhesion during Embryonic Development and Larval Immune Responses.”
Current Opinion in Cell Biology. Elsevier, 2015. https://doi.org/10.1016/j.ceb.2015.07.003.
ieee: A. Ratheesh, V. Belyaeva, and D. E. Siekhaus, “Drosophila immune cell migration
and adhesion during embryonic development and larval immune responses,” Current
Opinion in Cell Biology, vol. 36, no. 10. Elsevier, pp. 71–79, 2015.
ista: Ratheesh A, Belyaeva V, Siekhaus DE. 2015. Drosophila immune cell migration
and adhesion during embryonic development and larval immune responses. Current
Opinion in Cell Biology. 36(10), 71–79.
mla: Ratheesh, Aparna, et al. “Drosophila Immune Cell Migration and Adhesion during
Embryonic Development and Larval Immune Responses.” Current Opinion in Cell
Biology, vol. 36, no. 10, Elsevier, 2015, pp. 71–79, doi:10.1016/j.ceb.2015.07.003.
short: A. Ratheesh, V. Belyaeva, D.E. Siekhaus, Current Opinion in Cell Biology
36 (2015) 71–79.
date_created: 2018-12-11T11:53:36Z
date_published: 2015-10-01T00:00:00Z
date_updated: 2021-01-12T06:52:41Z
day: '01'
ddc:
- '573'
department:
- _id: DaSi
doi: 10.1016/j.ceb.2015.07.003
ec_funded: 1
file:
- access_level: open_access
checksum: bbb1ee39ca52929aefe4f48752b166ee
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:44Z
date_updated: 2020-07-14T12:45:13Z
file_id: '5098'
file_name: IST-2015-346-v1+1_Current_Opinion_Review_Ratheesh_et_al_2015.pdf
file_size: 1023680
relation: main_file
file_date_updated: 2020-07-14T12:45:13Z
has_accepted_license: '1'
intvolume: ' 36'
issue: '10'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '10'
oa: 1
oa_version: Published Version
page: 71 - 79
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: Current Opinion in Cell Biology
publication_status: published
publisher: Elsevier
publist_id: '5421'
pubrep_id: '346'
quality_controlled: '1'
scopus_import: 1
status: public
title: Drosophila immune cell migration and adhesion during embryonic development
and larval immune responses
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2015'
...
---
_id: '2025'
abstract:
- lang: eng
text: Small GTP-binding proteins of the Ras superfamily play diverse roles in intracellular
trafficking. Among them, the Rab, Arf, and Rho families function in successive
steps of vesicle transport, in forming vesicles from donor membranes, directing
vesicle trafficking toward target membranes and docking vesicles onto target membranes.
These proteins act as molecular switches that are controlled by a cycle of GTP
binding and hydrolysis regulated by guanine nucleotide exchange factors (GEFs)
and GTPase-activating proteins (GAPs). In this study we explored the role of GAPs
in the regulation of the endocytic pathway using fluorescently labeled yeast mating
pheromone α-factor. Among 25 non-essential GAP mutants, we found that deletion
of the GLO3 gene, encoding Arf-GAP protein, caused defective internalization of
fluorescently labeled α-factor. Quantitative analysis revealed that glo3Δ cells
show defective α-factor binding to the cell surface. Interestingly, Ste2p, the
α-factor receptor, was mis-localized from the plasma membrane to the vacuole in
glo3Δ cells. Domain deletion mutants of Glo3p revealed that a GAP-independent
function, as well as the GAP activity, of Glo3p is important for both α-factor
binding and Ste2p localization at the cell surface. Additionally, we found that
deletion of the GLO3 gene affects the size and number of Arf1p-residing Golgi
compartments and causes a defect in transport from the TGN to the plasma membrane.
Furthermore, we demonstrated that glo3Δ cells were defective in the late endosome-to-TGN
transport pathway, but not in the early endosome-to-TGN transport pathway. These
findings suggest novel roles for Arf-GAP Glo3p in endocytic recycling of cell
surface proteins.
author:
- first_name: Daiki
full_name: Kawada, Daiki
last_name: Kawada
- first_name: Hiromu
full_name: Kobayashi, Hiromu
last_name: Kobayashi
- first_name: Tsuyoshi
full_name: Tomita, Tsuyoshi
last_name: Tomita
- first_name: Eisuke
full_name: Nakata, Eisuke
last_name: Nakata
- first_name: Makoto
full_name: Nagano, Makoto
last_name: Nagano
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Junko
full_name: Toshima, Junko
last_name: Toshima
- first_name: Jiro
full_name: Toshimaa, Jiro
last_name: Toshimaa
citation:
ama: Kawada D, Kobayashi H, Tomita T, et al. The yeast Arf-GAP Glo3p is required
for the endocytic recycling of cell surface proteins. Biochimica et Biophysica
Acta - Molecular Cell Research. 2015;1853(1):144-156. doi:10.1016/j.bbamcr.2014.10.009
apa: Kawada, D., Kobayashi, H., Tomita, T., Nakata, E., Nagano, M., Siekhaus, D.
E., … Toshimaa, J. (2015). The yeast Arf-GAP Glo3p is required for the endocytic
recycling of cell surface proteins. Biochimica et Biophysica Acta - Molecular
Cell Research. Elsevier. https://doi.org/10.1016/j.bbamcr.2014.10.009
chicago: Kawada, Daiki, Hiromu Kobayashi, Tsuyoshi Tomita, Eisuke Nakata, Makoto
Nagano, Daria E Siekhaus, Junko Toshima, and Jiro Toshimaa. “The Yeast Arf-GAP
Glo3p Is Required for the Endocytic Recycling of Cell Surface Proteins.” Biochimica
et Biophysica Acta - Molecular Cell Research. Elsevier, 2015. https://doi.org/10.1016/j.bbamcr.2014.10.009.
ieee: D. Kawada et al., “The yeast Arf-GAP Glo3p is required for the endocytic
recycling of cell surface proteins,” Biochimica et Biophysica Acta - Molecular
Cell Research, vol. 1853, no. 1. Elsevier, pp. 144–156, 2015.
ista: Kawada D, Kobayashi H, Tomita T, Nakata E, Nagano M, Siekhaus DE, Toshima
J, Toshimaa J. 2015. The yeast Arf-GAP Glo3p is required for the endocytic recycling
of cell surface proteins. Biochimica et Biophysica Acta - Molecular Cell Research.
1853(1), 144–156.
mla: Kawada, Daiki, et al. “The Yeast Arf-GAP Glo3p Is Required for the Endocytic
Recycling of Cell Surface Proteins.” Biochimica et Biophysica Acta - Molecular
Cell Research, vol. 1853, no. 1, Elsevier, 2015, pp. 144–56, doi:10.1016/j.bbamcr.2014.10.009.
short: D. Kawada, H. Kobayashi, T. Tomita, E. Nakata, M. Nagano, D.E. Siekhaus,
J. Toshima, J. Toshimaa, Biochimica et Biophysica Acta - Molecular Cell Research
1853 (2015) 144–156.
date_created: 2018-12-11T11:55:17Z
date_published: 2015-01-01T00:00:00Z
date_updated: 2021-01-12T06:54:48Z
day: '01'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.1016/j.bbamcr.2014.10.009
file:
- access_level: open_access
checksum: 5bb328edebb6a91337cadd7d63f961b7
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:18Z
date_updated: 2020-07-14T12:45:25Z
file_id: '4936'
file_name: IST-2016-615-v1+1_BBAMCR.pdf
file_size: 926685
relation: main_file
file_date_updated: 2020-07-14T12:45:25Z
has_accepted_license: '1'
intvolume: ' 1853'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
page: 144 - 156
publication: Biochimica et Biophysica Acta - Molecular Cell Research
publication_status: published
publisher: Elsevier
publist_id: '5047'
pubrep_id: '615'
quality_controlled: '1'
scopus_import: 1
status: public
title: The yeast Arf-GAP Glo3p is required for the endocytic recycling of cell surface
proteins
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1853
year: '2015'
...
---
_id: '2024'
abstract:
- lang: eng
text: 'The yeast Rab5 homologue, Vps21p, is known to be involved both in the vacuolar
protein sorting (VPS) pathway from the trans-Golgi network to the vacuole, and
in the endocytic pathway from the plasma membrane to the vacuole. However, the
intracellular location at which these two pathways converge remains unclear. In
addition, the endocytic pathway is not completely blocked in yeast cells lacking
all Rab5 genes, suggesting the existence of an unidentified route that bypasses
the Rab5-dependent endocytic pathway. Here we show that convergence of the endocytic
and VPS pathways occurs upstream of the requirement for Vps21p in these pathways.
We also identify a previously unidentified endocytic pathway mediated by the AP-3
complex. Importantly, the AP-3-mediated pathway appears mostly intact in Rab5-disrupted
cells, and thus works as an alternative route to the vacuole/lysosome. We propose
that the endocytic traffic branches into two routes to reach the vacuole: a Rab5-dependent
VPS pathway and a Rab5-independent AP-3-mediated pathway.'
article_number: '3498'
author:
- first_name: Junko
full_name: Toshima, Junko
last_name: Toshima
- first_name: Show
full_name: Nishinoaki, Show
last_name: Nishinoaki
- first_name: Yoshifumi
full_name: Sato, Yoshifumi
last_name: Sato
- first_name: Wataru
full_name: Yamamoto, Wataru
last_name: Yamamoto
- first_name: Daiki
full_name: Furukawa, Daiki
last_name: Furukawa
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Akira
full_name: Sawaguchi, Akira
last_name: Sawaguchi
- first_name: Jiro
full_name: Toshima, Jiro
last_name: Toshima
citation:
ama: Toshima J, Nishinoaki S, Sato Y, et al. Bifurcation of the endocytic pathway
into Rab5-dependent and -independent transport to the vacuole. Nature Communications.
2014;5. doi:10.1038/ncomms4498
apa: Toshima, J., Nishinoaki, S., Sato, Y., Yamamoto, W., Furukawa, D., Siekhaus,
D. E., … Toshima, J. (2014). Bifurcation of the endocytic pathway into Rab5-dependent
and -independent transport to the vacuole. Nature Communications. Nature
Publishing Group. https://doi.org/10.1038/ncomms4498
chicago: Toshima, Junko, Show Nishinoaki, Yoshifumi Sato, Wataru Yamamoto, Daiki
Furukawa, Daria E Siekhaus, Akira Sawaguchi, and Jiro Toshima. “Bifurcation of
the Endocytic Pathway into Rab5-Dependent and -Independent Transport to the Vacuole.”
Nature Communications. Nature Publishing Group, 2014. https://doi.org/10.1038/ncomms4498.
ieee: J. Toshima et al., “Bifurcation of the endocytic pathway into Rab5-dependent
and -independent transport to the vacuole,” Nature Communications, vol.
5. Nature Publishing Group, 2014.
ista: Toshima J, Nishinoaki S, Sato Y, Yamamoto W, Furukawa D, Siekhaus DE, Sawaguchi
A, Toshima J. 2014. Bifurcation of the endocytic pathway into Rab5-dependent and
-independent transport to the vacuole. Nature Communications. 5, 3498.
mla: Toshima, Junko, et al. “Bifurcation of the Endocytic Pathway into Rab5-Dependent
and -Independent Transport to the Vacuole.” Nature Communications, vol.
5, 3498, Nature Publishing Group, 2014, doi:10.1038/ncomms4498.
short: J. Toshima, S. Nishinoaki, Y. Sato, W. Yamamoto, D. Furukawa, D.E. Siekhaus,
A. Sawaguchi, J. Toshima, Nature Communications 5 (2014).
date_created: 2018-12-11T11:55:16Z
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title: Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport
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