[{"author":[{"first_name":"Guifang","last_name":"Zeng","full_name":"Zeng, Guifang"},{"first_name":"Qing","full_name":"Sun, Qing","last_name":"Sun"},{"last_name":"Horta","full_name":"Horta, Sharona","id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc","first_name":"Sharona"},{"full_name":"Martínez-Alanis, Paulina R.","last_name":"Martínez-Alanis","first_name":"Paulina R."},{"last_name":"Wu","full_name":"Wu, Peng","first_name":"Peng"},{"first_name":"Jing","full_name":"Li, Jing","last_name":"Li"},{"first_name":"Shang","full_name":"Wang, Shang","last_name":"Wang"},{"last_name":"Ibáñez","full_name":"Ibáñez, Maria","id":"43C61214-F248-11E8-B48F-1D18A9856A87","first_name":"Maria","orcid":"0000-0001-5013-2843"},{"first_name":"Yanhong","last_name":"Tian","full_name":"Tian, Yanhong"},{"full_name":"Ci, Lijie","last_name":"Ci","first_name":"Lijie"},{"full_name":"Cabot, Andreu","last_name":"Cabot","first_name":"Andreu"}],"day":"21","oa_version":"None","issue":"4","abstract":[{"text":"Electrolyte additives are extensively validated effective in mitigating dendrite growth and parasitic reactions in aqueous zinc-ion batteries (AZIBs). Nonetheless, the mechanisms by which additives influence the formation and characteristics of the inorganic solid–electrolyte interphase (SEI) are not yet fully elucidated. Herein, we investigate how Zn(CF3COO)2 additives influence solvation structure and elucidate the mechanism by which these additives promote the dual reduction of anions. Through cryo-transmission electron microscopy analysis, we identified the SEI as a highly amorphous ZnS/ZnF2 phase. This amorphous hybrid SEI demonstrates exceptional stability, mechanical robustness, and high Zn2+ conductivity, effectively mitigating parasitic reactions and enhancing Zn plating/stripping reversibility. Even under elevated current densities, the Zn anode exhibits ultra-stable longevity and ultra-high reversibility. This study provides a comprehensive understanding of the intrinsic mechanisms governing solvation structure modulation that lead to the formation of amorphous hybrid SEI, underscoring their efficacy in enhancing the performance and durability of AZIBs.","lang":"eng"}],"publication":"Energy and Environmental Science","date_published":"2025-02-21T00:00:00Z","external_id":{"isi":["001389898000001"]},"doi":"10.1039/d4ee03750b","acknowledgement":"The authors acknowledge financial support from the Joint Fund of Henan Province Science and Technology R&D Program (235200810097) and the Generalitat de Catalunya (2021SGR01581). This research was supported by the Scientific Service Units (SSU) of ISTA Austria through resources provided by the Electron Microscopy Facility (EMF) and the Nanofabrication Facility (NFF). G. Z. and J. L. thank the China Scholarship Council (CSC) for the scholarship support.","publication_identifier":{"issn":["1754-5692"],"eissn":["1754-5706"]},"year":"2025","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2025-01-19T23:01:52Z","type":"journal_article","title":"Modulating the solvation structure to enhance amorphous solid electrolyte interface formation for ultra-stable aqueous zinc anode","language":[{"iso":"eng"}],"isi":1,"publication_status":"published","article_type":"original","page":"1683-1695","article_processing_charge":"No","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"NanoFab"}],"volume":18,"quality_controlled":"1","OA_type":"closed access","citation":{"short":"G. Zeng, Q. Sun, S. Horta, P.R. Martínez-Alanis, P. Wu, J. Li, S. Wang, M. Ibáñez, Y. Tian, L. Ci, A. Cabot, Energy and Environmental Science 18 (2025) 1683–1695.","ista":"Zeng G, Sun Q, Horta S, Martínez-Alanis PR, Wu P, Li J, Wang S, Ibáñez M, Tian Y, Ci L, Cabot A. 2025. Modulating the solvation structure to enhance amorphous solid electrolyte interface formation for ultra-stable aqueous zinc anode. Energy and Environmental Science. 18(4), 1683–1695.","mla":"Zeng, Guifang, et al. “Modulating the Solvation Structure to Enhance Amorphous Solid Electrolyte Interface Formation for Ultra-Stable Aqueous Zinc Anode.” <i>Energy and Environmental Science</i>, vol. 18, no. 4, Royal Society of Chemistry, 2025, pp. 1683–95, doi:<a href=\"https://doi.org/10.1039/d4ee03750b\">10.1039/d4ee03750b</a>.","apa":"Zeng, G., Sun, Q., Horta, S., Martínez-Alanis, P. R., Wu, P., Li, J., … Cabot, A. (2025). Modulating the solvation structure to enhance amorphous solid electrolyte interface formation for ultra-stable aqueous zinc anode. <i>Energy and Environmental Science</i>. Royal Society of Chemistry. <a href=\"https://doi.org/10.1039/d4ee03750b\">https://doi.org/10.1039/d4ee03750b</a>","ieee":"G. Zeng <i>et al.</i>, “Modulating the solvation structure to enhance amorphous solid electrolyte interface formation for ultra-stable aqueous zinc anode,” <i>Energy and Environmental Science</i>, vol. 18, no. 4. Royal Society of Chemistry, pp. 1683–1695, 2025.","chicago":"Zeng, Guifang, Qing Sun, Sharona Horta, Paulina R. Martínez-Alanis, Peng Wu, Jing Li, Shang Wang, et al. “Modulating the Solvation Structure to Enhance Amorphous Solid Electrolyte Interface Formation for Ultra-Stable Aqueous Zinc Anode.” <i>Energy and Environmental Science</i>. Royal Society of Chemistry, 2025. <a href=\"https://doi.org/10.1039/d4ee03750b\">https://doi.org/10.1039/d4ee03750b</a>.","ama":"Zeng G, Sun Q, Horta S, et al. Modulating the solvation structure to enhance amorphous solid electrolyte interface formation for ultra-stable aqueous zinc anode. <i>Energy and Environmental Science</i>. 2025;18(4):1683-1695. doi:<a href=\"https://doi.org/10.1039/d4ee03750b\">10.1039/d4ee03750b</a>"},"intvolume":"        18","publisher":"Royal Society of Chemistry","month":"02","date_updated":"2025-07-10T11:51:27Z","scopus_import":"1","department":[{"_id":"MaIb"}],"status":"public","_id":"18853"},{"quality_controlled":"1","volume":25,"OA_type":"hybrid","has_accepted_license":"1","citation":{"mla":"Hasler, Roger, et al. “Dual Electronic and Optical Monitoring of Biointerfaces by a Grating-Structured Coplanar-Gated Field-Effect Transistor.” <i>IEEE Sensors Journal</i>, vol. 25, no. 7, IEEE, 2025, pp. 10521–29, doi:<a href=\"https://doi.org/10.1109/jsen.2025.3533113\">10.1109/jsen.2025.3533113</a>.","apa":"Hasler, R., Livio, P. A., Bozdogan, A., Fossati, S., Hageneder, S., Montes-García, V., … Knoll, W. (2025). Dual electronic and optical monitoring of biointerfaces by a grating-structured coplanar-gated field-effect transistor. <i>IEEE Sensors Journal</i>. IEEE. <a href=\"https://doi.org/10.1109/jsen.2025.3533113\">https://doi.org/10.1109/jsen.2025.3533113</a>","short":"R. Hasler, P.A. Livio, A. Bozdogan, S. Fossati, S. Hageneder, V. Montes-García, J. Movilli, T. Moazzenzade, L. Loohuis, C. Reiner-Rozman, A. Tamayo, C. Fiedler, M. Ibáñez, C. Kleber, J. Huskens, J. Dostalek, P. Samorì, W. Knoll, IEEE Sensors Journal 25 (2025) 10521–10529.","ista":"Hasler R, Livio PA, Bozdogan A, Fossati S, Hageneder S, Montes-García V, Movilli J, Moazzenzade T, Loohuis L, Reiner-Rozman C, Tamayo A, Fiedler C, Ibáñez M, Kleber C, Huskens J, Dostalek J, Samorì P, Knoll W. 2025. Dual electronic and optical monitoring of biointerfaces by a grating-structured coplanar-gated field-effect transistor. IEEE Sensors Journal. 25(7), 10521–10529.","ama":"Hasler R, Livio PA, Bozdogan A, et al. Dual electronic and optical monitoring of biointerfaces by a grating-structured coplanar-gated field-effect transistor. <i>IEEE Sensors Journal</i>. 2025;25(7):10521-10529. doi:<a href=\"https://doi.org/10.1109/jsen.2025.3533113\">10.1109/jsen.2025.3533113</a>","chicago":"Hasler, Roger, Pietro A. Livio, Anil Bozdogan, Stefan Fossati, Simone Hageneder, Verónica Montes-García, Jacopo Movilli, et al. “Dual Electronic and Optical Monitoring of Biointerfaces by a Grating-Structured Coplanar-Gated Field-Effect Transistor.” <i>IEEE Sensors Journal</i>. IEEE, 2025. <a href=\"https://doi.org/10.1109/jsen.2025.3533113\">https://doi.org/10.1109/jsen.2025.3533113</a>.","ieee":"R. Hasler <i>et al.</i>, “Dual electronic and optical monitoring of biointerfaces by a grating-structured coplanar-gated field-effect transistor,” <i>IEEE Sensors Journal</i>, vol. 25, no. 7. IEEE, pp. 10521–10529, 2025."},"page":"10521-10529","article_processing_charge":"Yes (in subscription journal)","acknowledged_ssus":[{"_id":"EM-Fac"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"OA_place":"publisher","month":"04","intvolume":"        25","publisher":"IEEE","oa":1,"date_updated":"2026-02-16T11:50:01Z","status":"public","_id":"19037","scopus_import":"1","ddc":["540"],"department":[{"_id":"MaIb"}],"file_date_updated":"2025-12-30T07:59:13Z","oa_version":"Published Version","issue":"7","author":[{"last_name":"Hasler","full_name":"Hasler, Roger","first_name":"Roger"},{"first_name":"Pietro A.","last_name":"Livio","full_name":"Livio, Pietro A."},{"last_name":"Bozdogan","full_name":"Bozdogan, Anil","first_name":"Anil"},{"full_name":"Fossati, Stefan","last_name":"Fossati","first_name":"Stefan"},{"first_name":"Simone","full_name":"Hageneder, Simone","last_name":"Hageneder"},{"first_name":"Verónica","full_name":"Montes-García, Verónica","last_name":"Montes-García"},{"first_name":"Jacopo","full_name":"Movilli, Jacopo","last_name":"Movilli"},{"first_name":"Taghi","last_name":"Moazzenzade","full_name":"Moazzenzade, Taghi"},{"first_name":"Luna","last_name":"Loohuis","full_name":"Loohuis, Luna"},{"full_name":"Reiner-Rozman, Ciril","last_name":"Reiner-Rozman","first_name":"Ciril"},{"first_name":"Adrián","last_name":"Tamayo","full_name":"Tamayo, Adrián"},{"first_name":"Christine","id":"bd3fceba-dc74-11ea-a0a7-c17f71817366","full_name":"Fiedler, Christine","last_name":"Fiedler"},{"first_name":"Maria","id":"43C61214-F248-11E8-B48F-1D18A9856A87","full_name":"Ibáñez, Maria","last_name":"Ibáñez","orcid":"0000-0001-5013-2843"},{"first_name":"Christoph","full_name":"Kleber, Christoph","last_name":"Kleber"},{"first_name":"Jurriaan","last_name":"Huskens","full_name":"Huskens, Jurriaan"},{"full_name":"Dostalek, Jakub","last_name":"Dostalek","first_name":"Jakub"},{"full_name":"Samorì, Paolo","last_name":"Samorì","first_name":"Paolo"},{"first_name":"Wolfgang","last_name":"Knoll","full_name":"Knoll, Wolfgang"}],"PlanS_conform":"1","day":"01","publication":"IEEE Sensors Journal","file":[{"creator":"dernst","date_updated":"2025-12-30T07:59:13Z","success":1,"content_type":"application/pdf","date_created":"2025-12-30T07:59:13Z","file_size":2214584,"checksum":"9cdd4017025a3add6198ed84798319e8","file_name":"2025_IEEESensor_Hasler.pdf","file_id":"20887","relation":"main_file","access_level":"open_access"}],"external_id":{"isi":["001457747000001"]},"date_published":"2025-04-01T00:00:00Z","abstract":[{"lang":"eng","text":"We present a novel, portable sensor platform that enables concurrent monitoring of surface mass and charge density variations at thin biointerfaces. This platform combines a coplanar-gated field-effect transistor (FET) architecture with grating-coupled surface plasmon resonance (SPR), yielding an integrated disposable sensor chip prepared by nanoimprint and maskless photolithography techniques. The sensor chip design is suitable for scalable production and relies on reduced graphene oxide (rGO), serving as the FET’s semiconductor material for the electronic readout, and a metallic gate electrode surface that is corrugated with a multi-diffractive structure for optical probing with resonantly excited surface plasmons. Together with its integration in a compact instrumentation this results in a form factor optimized solution for dual-mode investigations without compromising the optical or electronic sensor performance. A poly-L-lysine (PLL) – based thin linker layer was deployed at the sensor surface to covalently attach azide-conjugated biomolecules by using incorporated “clickable” dibenzocyclooctyne (DBCO) moieties. Interestingly, the dual-mode measurements allow elucidating the role of the globular nature of the PLL chains when increasing the density of DBCO attached to their backbone, leading to PLL folding and internalization of DBCO moieties, and thus reducing the coupling yield for the used DNA oligomers. We envision that this platform can be employed to studying a range of other biointerface architectures and biomolecular interaction phenomena, which are inherently tied to mass and charge density variations."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2025-02-17T09:22:26Z","doi":"10.1109/jsen.2025.3533113","acknowledgement":"We thank the Electron Microscopy Facility at ISTA for their support with sputter coating the FO probes and NOSI GmbH for their support with 3D printing.","publication_identifier":{"issn":["1530-437X"],"eissn":["1558-1748"]},"year":"2025","publication_status":"published","article_type":"original","title":"Dual electronic and optical monitoring of biointerfaces by a grating-structured coplanar-gated field-effect transistor","type":"journal_article","isi":1,"language":[{"iso":"eng"}]},{"abstract":[{"text":"The practical implementation of aqueous zinc-ion batteries (AZIBs) is limited by uncontrolled zinc (Zn) dendrite growth during anode plating, compromising both safety and cycle life. Typically, Zn plating proceeds via 2D growth along the six equivalent prismatic [1010] directions of the hexagonal close-packed (HCP) Zn lattice, forming hexagonal platelets that promote dendrite formation. Here, an effective electrolyte engineering strategy is presented using rare-earth ions to regulate Zn plating. Combined multiscale experimental analyses and computational modeling reveal that these ions preferentially adsorb onto the prismatic {1010} facets, suppressing lateral epitaxial growth of the basal (0002) planes. This redirects Zn plating toward an apparent screw dislocation-driven growth along the [0001] axis. The resulting growth pathway, together with randomly oriented Zn nucleation, yields dense, uniform, and dendrite-free Zn layers with markedly improved cycling stability and high depth-of-discharge operation, thereby challenging the prevailing assumption that dendrite suppression requires (0002)-oriented growth parallel to the substrate. This work provides new mechanistic insights into Zn plating dynamics and establishes a scalable strategy for stable, dendrite-free Zn anodes in next-generation AZIBs.","lang":"eng"}],"publication":"Advanced Materials","external_id":{"pmid":["41025826"],"isi":["001583809400001"]},"date_published":"2025-09-30T00:00:00Z","author":[{"first_name":"Guifang","full_name":"Zeng, Guifang","last_name":"Zeng"},{"first_name":"Sharona","id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc","full_name":"Horta, Sharona","last_name":"Horta"},{"first_name":"Qing","last_name":"Sun","full_name":"Sun, Qing"},{"last_name":"Khan","full_name":"Khan, Malik Dilshad","first_name":"Malik Dilshad"},{"last_name":"Ibáñez","full_name":"Ibáñez, Maria","id":"43C61214-F248-11E8-B48F-1D18A9856A87","first_name":"Maria","orcid":"0000-0001-5013-2843"},{"first_name":"Yuhang","last_name":"Han","full_name":"Han, Yuhang"},{"first_name":"Shang","last_name":"Wang","full_name":"Wang, Shang"},{"full_name":"Li, Longqiu","last_name":"Li","first_name":"Longqiu"},{"full_name":"Ci, Lijie","last_name":"Ci","first_name":"Lijie"},{"last_name":"Tian","full_name":"Tian, Yanhong","first_name":"Yanhong"},{"first_name":"Andreu","last_name":"Cabot","full_name":"Cabot, Andreu"}],"day":"30","PlanS_conform":"1","pmid":1,"oa_version":"Published Version","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1002/adma.202510906"}],"title":"Crystal growth engineering for dendrite-free Zinc metal plating","type":"journal_article","project":[{"name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery","_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A"}],"isi":1,"language":[{"iso":"eng"}],"publication_status":"epub_ahead","article_type":"original","doi":"10.1002/adma.202510906","acknowledgement":"M.I. and S.H. acknowledge financial support from ISTA and the Werner Siemens Foundation. Q.S. acknowledges financial support from the European Union's Horizon Europe Research and Innovation Programme under the Marie Skłodowska-Curie Grant Agreement No. 101211154. This work was supported by the Generalitat de Catalunya (Grant No. 2021SGR01581), the National Natural Science Foundation of China (Grant Nos. 52125505 and 52475336), and the Joint Fund of Henan Province Science and Technology R&D Program (Grant No. 235200810097). Part of this research was carried out with support from the Scientific Service Units (SSU) of the Institute of Science and Technology Austria (ISTA), utilizing resources provided by the Electron Microscopy Facility (EMF) and the Nanofabrication Facility (NFF).","publication_identifier":{"issn":["0935-9648"],"eissn":["1521-4095"]},"article_number":"e10906","year":"2025","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2025-10-19T22:01:32Z","publisher":"Wiley","month":"09","OA_place":"publisher","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"acknowledged_ssus":[{"_id":"NanoFab"},{"_id":"EM-Fac"}],"article_processing_charge":"Yes (in subscription journal)","OA_type":"hybrid","quality_controlled":"1","citation":{"apa":"Zeng, G., Horta, S., Sun, Q., Khan, M. D., Ibáñez, M., Han, Y., … Cabot, A. (2025). Crystal growth engineering for dendrite-free Zinc metal plating. <i>Advanced Materials</i>. Wiley. <a href=\"https://doi.org/10.1002/adma.202510906\">https://doi.org/10.1002/adma.202510906</a>","mla":"Zeng, Guifang, et al. “Crystal Growth Engineering for Dendrite-Free Zinc Metal Plating.” <i>Advanced Materials</i>, e10906, Wiley, 2025, doi:<a href=\"https://doi.org/10.1002/adma.202510906\">10.1002/adma.202510906</a>.","short":"G. Zeng, S. Horta, Q. Sun, M.D. Khan, M. Ibáñez, Y. Han, S. Wang, L. Li, L. Ci, Y. Tian, A. Cabot, Advanced Materials (2025).","ista":"Zeng G, Horta S, Sun Q, Khan MD, Ibáñez M, Han Y, Wang S, Li L, Ci L, Tian Y, Cabot A. 2025. Crystal growth engineering for dendrite-free Zinc metal plating. Advanced Materials., e10906.","chicago":"Zeng, Guifang, Sharona Horta, Qing Sun, Malik Dilshad Khan, Maria Ibáñez, Yuhang Han, Shang Wang, et al. “Crystal Growth Engineering for Dendrite-Free Zinc Metal Plating.” <i>Advanced Materials</i>. Wiley, 2025. <a href=\"https://doi.org/10.1002/adma.202510906\">https://doi.org/10.1002/adma.202510906</a>.","ama":"Zeng G, Horta S, Sun Q, et al. Crystal growth engineering for dendrite-free Zinc metal plating. <i>Advanced Materials</i>. 2025. doi:<a href=\"https://doi.org/10.1002/adma.202510906\">10.1002/adma.202510906</a>","ieee":"G. Zeng <i>et al.</i>, “Crystal growth engineering for dendrite-free Zinc metal plating,” <i>Advanced Materials</i>. Wiley, 2025."},"has_accepted_license":"1","scopus_import":"1","department":[{"_id":"MaIb"}],"ddc":["530"],"status":"public","_id":"20496","date_updated":"2025-12-01T12:56:48Z","oa":1},{"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"EM-Fac"},{"_id":"ScienComp"},{"_id":"PreCl"}],"article_processing_charge":"No","day":"10","author":[{"id":"36062FEC-F248-11E8-B48F-1D18A9856A87","first_name":"Annamaria","last_name":"Hlavata","full_name":"Hlavata, Annamaria"},{"first_name":"Benjamin","full_name":"Neuditschko, Benjamin","last_name":"Neuditschko"},{"full_name":"Schellhaas, Ulla","last_name":"Schellhaas","first_name":"Ulla"},{"first_name":"Clemens","last_name":"Plaschka","full_name":"Plaschka, Clemens"},{"first_name":"Franz","last_name":"Herzog","full_name":"Herzog, Franz"},{"orcid":"0000-0003-0893-7036","id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","first_name":"Carrie A","last_name":"Bernecky","full_name":"Bernecky, Carrie A"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.64898/2025.12.10.692585"}],"citation":{"mla":"Hlavata, Annamaria, et al. <i>Structure of Cytoplasmic RNA Polymerase II</i>. bioRxiv, 2025, doi:<a href=\"https://doi.org/10.64898/2025.12.10.692585\">10.64898/2025.12.10.692585</a>.","apa":"Hlavata, A., Neuditschko, B., Schellhaas, U., Plaschka, C., Herzog, F., &#38; Bernecky, C. (2025). Structure of cytoplasmic RNA polymerase II. bioRxiv. <a href=\"https://doi.org/10.64898/2025.12.10.692585\">https://doi.org/10.64898/2025.12.10.692585</a>","ista":"Hlavata A, Neuditschko B, Schellhaas U, Plaschka C, Herzog F, Bernecky C. 2025. Structure of cytoplasmic RNA polymerase II. <a href=\"https://doi.org/10.64898/2025.12.10.692585\">10.64898/2025.12.10.692585</a>.","short":"A. Hlavata, B. Neuditschko, U. Schellhaas, C. Plaschka, F. Herzog, C. Bernecky, (2025).","ama":"Hlavata A, Neuditschko B, Schellhaas U, Plaschka C, Herzog F, Bernecky C. Structure of cytoplasmic RNA polymerase II. 2025. doi:<a href=\"https://doi.org/10.64898/2025.12.10.692585\">10.64898/2025.12.10.692585</a>","chicago":"Hlavata, Annamaria, Benjamin Neuditschko, Ulla Schellhaas, Clemens Plaschka, Franz Herzog, and Carrie Bernecky. “Structure of Cytoplasmic RNA Polymerase II.” bioRxiv, 2025. <a href=\"https://doi.org/10.64898/2025.12.10.692585\">https://doi.org/10.64898/2025.12.10.692585</a>.","ieee":"A. Hlavata, B. Neuditschko, U. Schellhaas, C. Plaschka, F. Herzog, and C. Bernecky, “Structure of cytoplasmic RNA polymerase II.” bioRxiv, 2025."},"oa_version":"None","abstract":[{"lang":"eng","text":"RNA polymerase II (Pol II) must be assembled in the cytoplasm before it enters the nucleus, where it transcribes protein-coding genes. Although transcription by Pol II is intensively studied, how this central multi-subunit enzyme is made and the role of dedicated factors remains unclear. Here, we report the integrative structural analysis of a native human Pol II from the cytoplasm captured near the end of biogenesis. The complex contained Gdown1 and three biogenesis factors – RPAP2 and the critical small GTPases GPN1 and GPN3. Cryo-EM analysis of the complex revealed how Gdown1 and RPAP2 associate with Pol II and prevent the premature association of transcription factors. Further biochemical and cryo-EM analysis revealed how RPAP2 recruits GPN1–GPN3 to the complex, and how the assembly of the RPAP2–GPN1–GPN3 complex is controlled by GTP hydrolysis. The combined results uncover a network of interactions that chaperone cytoplasmic Pol II to prevent aberrant interactions, reveal a GTP-controlled switch during the final stages of Pol II biogenesis, and suggest a general mechanism for the action of GPN-loop GTPase family of enzymes."}],"publisher":"bioRxiv","date_published":"2025-12-10T00:00:00Z","month":"12","year":"2025","date_updated":"2025-12-15T09:48:22Z","doi":"10.64898/2025.12.10.692585","acknowledgement":"We thank A. Salmazo for assistance with Pol II purification. We thank staff at the VBCF Proteomics facility for immunoprecipitation-mass spectrometry analysis, and J.A. Stopp for assistance with IP-MS data visualization. This research was further supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by the Lab Support Facility (LSF), Electron Microscopy (EMF), Scientific Computing (SciComp), and the Preclinical Facility (PCF).","oa":1,"date_created":"2025-12-11T13:33:27Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"department":[{"_id":"CaBe"}],"title":"Structure of cytoplasmic RNA polymerase II","type":"preprint","_id":"20804","corr_author":"1","status":"public","publication_status":"published"},{"ddc":["540"],"department":[{"_id":"MaIb"}],"scopus_import":"1","_id":"20851","status":"public","date_updated":"2025-12-29T10:15:43Z","publisher":"Wiley","OA_place":"publisher","month":"12","article_processing_charge":"Yes","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"NanoFab"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"has_accepted_license":"1","citation":{"mla":"Chang, Xingqi, et al. “Mitigating the Rock-Salt Phase Transformation in Disordered LNMO through Synergetic Solid-State AlF3/LiF Modifications.” <i>Advanced Science</i>, e15962, Wiley, 2025, doi:<a href=\"https://doi.org/10.1002/advs.202515962\">10.1002/advs.202515962</a>.","apa":"Chang, X., Escudero, C., Black, A. P., Horta, S., Martínez, E., Lu, X., … Cabot, A. (2025). Mitigating the rock-salt phase transformation in disordered LNMO through synergetic solid-state AlF3/LiF modifications. <i>Advanced Science</i>. Wiley. <a href=\"https://doi.org/10.1002/advs.202515962\">https://doi.org/10.1002/advs.202515962</a>","short":"X. Chang, C. Escudero, A.P. Black, S. Horta, E. Martínez, X. Lu, J. Llorca, M. Ibáñez, J.J. Biendicho, A. Cabot, Advanced Science (2025).","ista":"Chang X, Escudero C, Black AP, Horta S, Martínez E, Lu X, Llorca J, Ibáñez M, Biendicho JJ, Cabot A. 2025. Mitigating the rock-salt phase transformation in disordered LNMO through synergetic solid-state AlF3/LiF modifications. Advanced Science., e15962.","chicago":"Chang, Xingqi, Carlos Escudero, Ashley P. Black, Sharona Horta, Elías Martínez, Xuan Lu, Jordi Llorca, Maria Ibáñez, Jordi Jacas Biendicho, and Andreu Cabot. “Mitigating the Rock-Salt Phase Transformation in Disordered LNMO through Synergetic Solid-State AlF3/LiF Modifications.” <i>Advanced Science</i>. Wiley, 2025. <a href=\"https://doi.org/10.1002/advs.202515962\">https://doi.org/10.1002/advs.202515962</a>.","ama":"Chang X, Escudero C, Black AP, et al. Mitigating the rock-salt phase transformation in disordered LNMO through synergetic solid-state AlF3/LiF modifications. <i>Advanced Science</i>. 2025. doi:<a href=\"https://doi.org/10.1002/advs.202515962\">10.1002/advs.202515962</a>","ieee":"X. Chang <i>et al.</i>, “Mitigating the rock-salt phase transformation in disordered LNMO through synergetic solid-state AlF3/LiF modifications,” <i>Advanced Science</i>. Wiley, 2025."},"OA_type":"gold","quality_controlled":"1","language":[{"iso":"eng"}],"project":[{"_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A","name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery"}],"type":"journal_article","title":"Mitigating the rock-salt phase transformation in disordered LNMO through synergetic solid-state AlF3/LiF modifications","article_type":"original","DOAJ_listed":"1","publication_status":"epub_ahead","year":"2025","publication_identifier":{"eissn":["2198-3844"]},"article_number":"e15962","acknowledgement":"This work was supported by the European Commission-financed project IntelLigent (HORIZON-CL5-2021-D2-01-02) with project ID number 101069765. In collaboration with ALBA staff, the operando SXRD and XAS experiments were performed at BL-16-NOTOS beamline at ALBA Synchrotron Light Source (experiment number: 2023097765). This research was supported by the Scientific Service Units (SSU) of the Institute of Science and Technology Austria (ISTA) through resources provided by the Electron Microscopy Facility (EMF) and the Nanofabrication Facility (NFF), and M.I. and S.H. acknowledge financial support from ISTA and the Werner Siemens Foundation. Jordi Jacas Biendicho acknowledges the fellowship RYC2021-034994-I, funded by MICIU/AEI/10.13039/501100011033 and the European Union «NextGenerationEU»/PRTR». Jordi Llorca is a Serra Húnter Fellow and is grateful to projects MICIN/AEI/FEDER PID2021-124572OB-C31 and Maria de Maeztu Units of Excellence Programme CEX2023-001300-M, and GC 2021 SGR 01061.","doi":"10.1002/advs.202515962","date_created":"2025-12-21T23:01:35Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"High-voltage disordered spinel LiNi0.5Mn1.5O4 is a promising cathode material for high power density in lithium-ion batteries. However, it suffers from poor cycle life associated with the rock-salt phase transformation. This study presents a straightforward synthesis approach to enhance the electrochemical performance of LiNi0.5Mn1.5O4 through a synergistic solid-state modification with LiF and AlF3. This dual modification promotes rapid Li⁺ diffusion, enables near-complete delithiation/lithiation, approaching the theoretical capacity of disordered LiNi0.5Mn1.5O4, and, more importantly, effectively mitigates the formation of the rock-salt phase, thereby enhancing structural stability, as confirmed by operando X-ray absorption spectroscopy (XAS) and synchrotron X-ray diffraction (SXRD). As a result, the optimized LiNi0.5Mn1.5O4 (10 mg AlF3 + 30 mg LiF) delivers high reversible capacities of 142.1, 139.1, 129.2, 121.6, 110.3, 93.5, and 76.1 mAh∙g−1 at 0.2C, 0.5C, 1.0C, 2.0C, 3.0C, 4.0C, and 5.0C, respectively. Full cells using graphite as the anode and a high-loading cathode exhibit excellent cycling performance. They retain 80% of their capacity after 200 cycles at 0.5C within a voltage window of 3.5–4.9 V with cathode loading of 11 mg∙cm−2. The findings of this study will significantly advance high-power LiNi0.5Mn1.5O4 materials, offering improved battery life and thereby enhancing their potential for practical applications."}],"date_published":"2025-12-12T00:00:00Z","publication":"Advanced Science","PlanS_conform":"1","day":"12","author":[{"full_name":"Chang, Xingqi","last_name":"Chang","first_name":"Xingqi"},{"first_name":"Carlos","last_name":"Escudero","full_name":"Escudero, Carlos"},{"first_name":"Ashley P.","last_name":"Black","full_name":"Black, Ashley P."},{"full_name":"Horta, Sharona","last_name":"Horta","first_name":"Sharona","id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc"},{"full_name":"Martínez, Elías","last_name":"Martínez","first_name":"Elías"},{"last_name":"Lu","full_name":"Lu, Xuan","first_name":"Xuan"},{"first_name":"Jordi","full_name":"Llorca, Jordi","last_name":"Llorca"},{"full_name":"Ibáñez, Maria","last_name":"Ibáñez","first_name":"Maria","id":"43C61214-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5013-2843"},{"full_name":"Biendicho, Jordi Jacas","last_name":"Biendicho","first_name":"Jordi Jacas"},{"first_name":"Andreu","last_name":"Cabot","full_name":"Cabot, Andreu"}],"oa_version":"Published Version"},{"date_created":"2024-09-08T10:29:06Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","publication_identifier":{"eissn":["1545-9985"],"issn":["1545-9993"]},"doi":"10.1038/s41594-024-01390-8","oaworkid":1,"acknowledgement":"This work was funded by the Institute of Science and Technology Austria (ISTA) and the Austrian Science Fund (grant P31445 to F.K.M.S.). Access to high-resolution cryo-ET data acquisition at European Molecular Biology Laboratory (EMBL) Heidelberg was supported through the EMBL cryo-EM platform. We thank V.-V. Hodirnau at ISTA and W. Hagen and F. Weis at EMBL Heidelberg for support in cryo-ET data acquisition. This research was also supported by the scientific service units of ISTA through resources provided by Scientific Computing, the Life Science Facility, and the EM Facility. L.M.M. was supported by National Institutes of Health grants R01 GM151775 and R21 DE032878 and by the University of Minnesota Masonic Cancer Center. D.P. was supported by the DOC doctoral fellowship program of the Austrian Academy of Sciences. R.A.D was supported by the National Institute of Allergy and Infectious Diseases (grant R01AI147890). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. Specifically, we also want to thank A. Schlögl for computational support and J. Hansen and V. Vogt for critical comments on the manuscript. We also thank the other members of the Schur lab for helpful discussions and experimental advice.","article_type":"original","publication_status":"published","language":[{"iso":"eng"}],"isi":1,"project":[{"name":"Structural conservation and diversity in retroviral capsid","grant_number":"P31445","_id":"26736D6A-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"_id":"9B9C98E0-BA93-11EA-9121-9846C619BF3A","grant_number":"25762","name":"Structural characterization of spumavirus capsid assemblies to understand conserved Ortervirales assembly mechanisms"}],"title":"Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice","type":"journal_article","pmid":1,"oa_version":"Published Version","APC_amount":"12348 EUR","day":"01","author":[{"full_name":"Obr, Martin","last_name":"Obr","first_name":"Martin","id":"4741CA5A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1756-6564"},{"full_name":"Percipalle, Mathias","last_name":"Percipalle","first_name":"Mathias","id":"4986e21c-eb97-11eb-a6c2-a4ef0b629971"},{"last_name":"Chernikova","full_name":"Chernikova, Darya","id":"7dbaf460-fa9e-11eb-b0ca-bc7c7ff21ad0","first_name":"Darya"},{"first_name":"Huixin","full_name":"Yang, Huixin","last_name":"Yang"},{"first_name":"Andreas","id":"3A18A7B8-F248-11E8-B48F-1D18A9856A87","full_name":"Thader, Andreas","last_name":"Thader"},{"last_name":"Pinke","full_name":"Pinke, Gergely","id":"4D5303E6-F248-11E8-B48F-1D18A9856A87","first_name":"Gergely"},{"last_name":"Porley","full_name":"Porley, Dario J","id":"2FD6EA6C-F248-11E8-B48F-1D18A9856A87","first_name":"Dario J"},{"first_name":"Louis M.","last_name":"Mansky","full_name":"Mansky, Louis M."},{"first_name":"Robert A.","full_name":"Dick, Robert A.","last_name":"Dick"},{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM","last_name":"Schur","full_name":"Schur, Florian KM","orcid":"0000-0003-4790-8078"}],"file":[{"relation":"main_file","access_level":"open_access","file_name":"2025_NatureStrucBio_Obr.pdf","file_id":"19608","checksum":"c641ad94afb28917b20425db676fc3ee","file_size":13724041,"success":1,"content_type":"application/pdf","date_created":"2025-04-23T07:02:33Z","date_updated":"2025-04-23T07:02:33Z","creator":"dernst"}],"external_id":{"pmid":["39242978"],"isi":["001306564000001"],"oaworkid":["W4402316284"]},"date_published":"2025-02-01T00:00:00Z","publication":"Nature Structural & Molecular Biology","abstract":[{"text":"Human T cell leukemia virus type 1 (HTLV-1) immature particles differ in morphology from other retroviruses, suggesting a distinct way of assembly. Here we report the results of cryo-electron tomography studies of HTLV-1 virus-like particles assembled in vitro, as well as derived from cells. This work shows that HTLV-1 uses a distinct mechanism of Gag–Gag interactions to form the immature viral lattice. Analysis of high-resolution structural information from immature capsid (CA) tubular arrays reveals that the primary stabilizing component in HTLV-1 is the N-terminal domain of CA. Mutagenesis analysis supports this observation. This distinguishes HTLV-1 from other retroviruses, in which the stabilization is provided primarily by the C-terminal domain of CA. These results provide structural details of the quaternary arrangement of Gag for an immature deltaretrovirus and this helps explain why HTLV-1 particles are morphologically distinct.","lang":"eng"}],"oa":1,"date_updated":"2026-03-16T12:55:18Z","_id":"17884","corr_author":"1","status":"public","ddc":["570"],"file_date_updated":"2025-04-23T07:02:33Z","department":[{"_id":"FlSc"},{"_id":"LeSa"}],"scopus_import":"1","has_accepted_license":"1","citation":{"ista":"Obr M, Percipalle M, Chernikova D, Yang H, Thader A, Pinke G, Porley Esteves D, Mansky LM, Dick RA, Schur FK. 2025. Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice. Nature Structural &#38; Molecular Biology. 32, 268–276.","short":"M. Obr, M. Percipalle, D. Chernikova, H. Yang, A. Thader, G. Pinke, D. Porley Esteves, L.M. Mansky, R.A. Dick, F.K. Schur, Nature Structural &#38; Molecular Biology 32 (2025) 268–276.","mla":"Obr, Martin, et al. “Distinct Stabilization of the Human T Cell Leukemia Virus Type 1 Immature Gag Lattice.” <i>Nature Structural &#38; Molecular Biology</i>, vol. 32, Springer Nature, 2025, pp. 268–76, doi:<a href=\"https://doi.org/10.1038/s41594-024-01390-8\">10.1038/s41594-024-01390-8</a>.","apa":"Obr, M., Percipalle, M., Chernikova, D., Yang, H., Thader, A., Pinke, G., … Schur, F. K. (2025). Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice. <i>Nature Structural &#38; Molecular Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41594-024-01390-8\">https://doi.org/10.1038/s41594-024-01390-8</a>","ieee":"M. Obr <i>et al.</i>, “Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice,” <i>Nature Structural &#38; Molecular Biology</i>, vol. 32. Springer Nature, pp. 268–276, 2025.","ama":"Obr M, Percipalle M, Chernikova D, et al. Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice. <i>Nature Structural &#38; Molecular Biology</i>. 2025;32:268-276. doi:<a href=\"https://doi.org/10.1038/s41594-024-01390-8\">10.1038/s41594-024-01390-8</a>","chicago":"Obr, Martin, Mathias Percipalle, Darya Chernikova, Huixin Yang, Andreas Thader, Gergely Pinke, Darío Porley Esteves, Louis M. Mansky, Robert A. Dick, and Florian KM Schur. “Distinct Stabilization of the Human T Cell Leukemia Virus Type 1 Immature Gag Lattice.” <i>Nature Structural &#38; Molecular Biology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41594-024-01390-8\">https://doi.org/10.1038/s41594-024-01390-8</a>."},"quality_controlled":"1","volume":32,"OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"page":"268-276","OA_place":"publisher","month":"02","publisher":"Springer Nature","intvolume":"        32"},{"month":"08","place":"New York","publisher":"Springer Nature","citation":{"apa":"Kleindienst, D., Costanzo, T., &#38; Shigemoto, R. (2024). Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In J. H. R. Lübke &#38; A. Rollenhagen (Eds.), <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i> (1st ed., pp. 123–137). New York: Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">https://doi.org/10.1007/978-1-0716-4019-7_8</a>","mla":"Kleindienst, David, et al. “Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning.” <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>, edited by Joachim H.R.  Lübke and Astrid Rollenhagen, 1st ed., Springer Nature, 2024, pp. 123–37, doi:<a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">10.1007/978-1-0716-4019-7_8</a>.","ista":"Kleindienst D, Costanzo T, Shigemoto R. 2024.Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In: New Aspects in Analyzing the Synaptic Organization of the Brain. Neuromethods, , 123–137.","short":"D. Kleindienst, T. Costanzo, R. Shigemoto, in:, J.H.R. Lübke, A. Rollenhagen (Eds.), New Aspects in Analyzing the Synaptic Organization of the Brain, 1st ed., Springer Nature, New York, 2024, pp. 123–137.","chicago":"Kleindienst, David, Tommaso Costanzo, and Ryuichi Shigemoto. “Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning.” In <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>, edited by Joachim H.R.  Lübke and Astrid Rollenhagen, 1st ed., 123–37. New York: Springer Nature, 2024. <a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">https://doi.org/10.1007/978-1-0716-4019-7_8</a>.","ama":"Kleindienst D, Costanzo T, Shigemoto R. Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In: Lübke JHR, Rollenhagen A, eds. <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>. 1st ed. New York: Springer Nature; 2024:123-137. doi:<a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">10.1007/978-1-0716-4019-7_8</a>","ieee":"D. Kleindienst, T. Costanzo, and R. Shigemoto, “Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning,” in <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>, 1st ed., J. H. R. Lübke and A. Rollenhagen, Eds. New York: Springer Nature, 2024, pp. 123–137."},"quality_controlled":"1","article_processing_charge":"No","acknowledged_ssus":[{"_id":"EM-Fac"}],"ec_funded":1,"page":"123-137","_id":"18052","corr_author":"1","status":"public","department":[{"_id":"EM-Fac"},{"_id":"RySh"}],"scopus_import":"1","date_updated":"2025-04-14T07:27:15Z","date_published":"2024-08-27T00:00:00Z","publication":"New Aspects in Analyzing the Synaptic Organization of the Brain","abstract":[{"lang":"eng","text":"Sodium dodecyl sulfate-digested freeze-fracture replica labeling (SDS-FRL) is an electron microscope (EM) sample preparation technique which allows for high-resolution visualization of membrane proteins with high sensitivity. However, image acquisition of specific replica profiles such as synapses in a large field of EM view needs a valid experience and a long time for manual searching. Here, we describe how to utilize deep learning for automatizing image acquisition of specific profiles of interest in replica samples. This protocol facilitates the labor-intensive collection of EM images, in particular for rare profiles. We provide instructions for using SerialEM image acquisition software in conjunction with object detection by our newly developed deep learning software DarEM, to automatically acquire tilt series of all synapses in a selected region. We then show how to perform a mostly automated analysis of gold particle labeling in the acquired images by utilizing Darea software."}],"oa_version":"None","day":"27","author":[{"full_name":"Kleindienst, David","last_name":"Kleindienst","first_name":"David","id":"42E121A4-F248-11E8-B48F-1D18A9856A87"},{"id":"D93824F4-D9BA-11E9-BB12-F207E6697425","first_name":"Tommaso","last_name":"Costanzo","full_name":"Costanzo, Tommaso","orcid":"0000-0001-9732-3815"},{"full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444"}],"edition":"1","publication_status":"published","language":[{"iso":"eng"}],"title":"Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning","type":"book_chapter","project":[{"_id":"25CA28EA-B435-11E9-9278-68D0E5697425","grant_number":"694539","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour","call_identifier":"H2020"}],"alternative_title":["Neuromethods"],"date_created":"2024-09-10T12:32:38Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"eissn":["1940-6045"],"eisbn":["9781071640197"],"issn":["0893-2336"],"isbn":["9781071640180"]},"year":"2024","editor":[{"last_name":"Lübke","full_name":"Lübke, Joachim H.R. ","first_name":"Joachim H.R. "},{"full_name":"Rollenhagen, Astrid","last_name":"Rollenhagen","first_name":"Astrid"}],"acknowledgement":"This research was supported by the European Research Council Advanced Grant 694539 to RS and by the Scientific Service Units of IST Austria through resources provided by the Electron Microscopy Facility.","doi":"10.1007/978-1-0716-4019-7_8"},{"citation":{"short":"D. Porley Esteves, Structural Characterization of Spumavirus Capsid Assemblies, Institute of Science and Technology Austria, 2024.","ista":"Porley Esteves D. 2024. Structural characterization of spumavirus capsid assemblies. Institute of Science and Technology Austria.","mla":"Porley Esteves, Darío. <i>Structural Characterization of Spumavirus Capsid Assemblies</i>. Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/at:ista:18101\">10.15479/at:ista:18101</a>.","apa":"Porley Esteves, D. (2024). <i>Structural characterization of spumavirus capsid assemblies</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:18101\">https://doi.org/10.15479/at:ista:18101</a>","ieee":"D. Porley Esteves, “Structural characterization of spumavirus capsid assemblies,” Institute of Science and Technology Austria, 2024.","ama":"Porley Esteves D. Structural characterization of spumavirus capsid assemblies. 2024. doi:<a href=\"https://doi.org/10.15479/at:ista:18101\">10.15479/at:ista:18101</a>","chicago":"Porley Esteves, Darío. “Structural Characterization of Spumavirus Capsid Assemblies.” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/at:ista:18101\">https://doi.org/10.15479/at:ista:18101</a>."},"has_accepted_license":"1","article_processing_charge":"No","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"ec_funded":1,"page":"131","month":"09","OA_place":"publisher","publisher":"Institute of Science and Technology Austria","supervisor":[{"first_name":"Florian KM","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","full_name":"Schur, Florian KM","last_name":"Schur","orcid":"0000-0003-4790-8078"}],"oa":1,"date_updated":"2026-04-07T13:21:01Z","corr_author":"1","_id":"18101","status":"public","file_date_updated":"2025-03-25T23:30:03Z","department":[{"_id":"GradSch"},{"_id":"FlSc"}],"ddc":["570"],"degree_awarded":"PhD","oa_version":"Published Version","day":"26","author":[{"id":"2FD6EA6C-F248-11E8-B48F-1D18A9856A87","first_name":"Dario J","last_name":"Porley","full_name":"Porley, Dario J"}],"date_published":"2024-09-26T00:00:00Z","file":[{"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2024-09-26T13:40:33Z","file_size":14213128,"creator":"dporley","date_updated":"2025-03-25T23:30:03Z","file_id":"18149","file_name":"PhD_thesis_DPorley_final_20240919.docx","relation":"source_file","access_level":"closed","embargo_to":"open_access","checksum":"3b8b0bacfe61112f3852744f3170e468"},{"checksum":"6c3a652a8eede874118e11d66a63652f","file_name":"PhD_thesis_DPorley_final_20240926_pdfa1.pdf","file_id":"18150","access_level":"open_access","embargo":"2025-03-25","relation":"main_file","creator":"dporley","date_updated":"2025-03-25T23:30:03Z","content_type":"application/pdf","date_created":"2024-09-26T13:41:39Z","file_size":18583031}],"abstract":[{"text":"The Retroviridae family consists of two sub-families, the Orthoretrovirinae and the\r\nSpumaretrovirinae. The Orthoretroviruses contain important human pathogens, such as the\r\nhuman immunodeficiency virus 1 (HIV-1). They also harbor other retrovirus species which\r\nare regularly used as model systems to study the retroviral life cycle. The main structural\r\ncomponent of the retroviruses, is the Gag protein and its truncation derivatives occurring\r\nduring viral maturation. Orthoretroviral Gag assemblies have been extensively studied to\r\nunderstand the interactions that confer stability and morphology to viral particles.\r\nThe Spumaretrovirinae subfamily represent an early diverging branch of the Retroviridae.\r\nIts members, the Foamy viruses (FV), share most of the conventional features found in\r\nretroviruses. However, they also possess multiple characteristics that make them unique. In\r\nparticular, FV Gag does not get extensively cleaved as in orthoretroviruses. Hence, the Gag\r\narchitecture deviates from the canonical domain arrangement in FV. They also exhibit a\r\npeculiar particle morphology, having no apparent immature state and a seemingly\r\nicosahedral mature particle. Due to this, many fundamental questions on FV structural\r\nassembly mechanisms remain open. To answer these questions, was the main focus of this\r\nthesis.\r\nMainly, it is not known how FV assemble their core in a virus particle and what are the\r\nimportant assembly interaction sites within said core. What is the minimum assembly\r\ncompetent domain of FV Gag? Is there a morphological change in the assembly type of FVGag lattices? If so, what is defining these morphological shifts? Finally, it would be\r\ninteresting to know what is the evolutionary relationship between FV and the rest of the\r\nretrotranscribing elements, from a structural point of view?\r\nTo answer these questions, membrane-enveloped mammalian cell-derived FV virus-like\r\nparticles (VLPs) were produced. Cryo-electron tomography (cryo-ET) analysis suggested\r\nthese FV VLPs do not form a canonical retroviral Gag lattice structure, which is in line with\r\nearlier observations. To further evaluate FV Gag assembly competence and morphology,\r\nthe first bacterial cell-derived in vitro VLP assembly system was designed and optimized.\r\nUsing this system with different truncation variants, the minimum assembly competent\r\ndomain of FV Gag was found to be the putative CA300-477 domain. Varying VLP\r\nmorphologies were also observed and strongly suggested residues upstream of CA300-477\r\nplay a role in morphology determination. Finally, a combined cryo-electron microscopy (cryoEM) and cryo-ET approach was taken to analyze tubular assemblies from the minimal\r\nassembly competent domain. This revealed an unexpectedly unique non-canonical\r\nassembly architecture. Three novel lattice stabilizing interfaces were described which\r\nproved to be as unique as the lattice arrangement. Comparison to a newly published FV CA\r\ncore structure revealed the CA-CA interactions in the atypical assembly do not recapitulate\r\nwhat is described for the FV core lattice. However, the new in vitro VLP assembly system\r\nobtained in this thesis also provides an exciting opportunity to study still unresolved FV\r\nassembly features in a potentially facilitated approach compared to conventional methods.\r\nIn summary, this work provided a deeper understanding of the basic FV Gag assembly unit,\r\nas well as presenting the first FV Gag-derived in vitro VLP assembly system. This system\r\nreveals a novel and unique assembly architecture among retroviral in vitro assemblies.","lang":"eng"}],"alternative_title":["ISTA Thesis"],"date_created":"2024-09-20T10:21:03Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-041-1"]},"year":"2024","doi":"10.15479/at:ista:18101","publication_status":"published","language":[{"iso":"eng"}],"title":"Structural characterization of spumavirus capsid assemblies","type":"dissertation","project":[{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","name":"International IST Doctoral Program","call_identifier":"H2020"},{"_id":"9B9C98E0-BA93-11EA-9121-9846C619BF3A","grant_number":"25762","name":"Structural characterization of spumavirus capsid assemblies to understand conserved Ortervirales assembly mechanisms"}]},{"corr_author":"1","_id":"18477","status":"public","department":[{"_id":"GradSch"},{"_id":"CaBe"}],"file_date_updated":"2025-10-29T23:30:02Z","ddc":["572"],"degree_awarded":"PhD","oa":1,"date_updated":"2026-04-07T13:23:59Z","month":"10","OA_place":"publisher","publisher":"Institute of Science and Technology Austria","supervisor":[{"full_name":"Bernecky, Carrie A","last_name":"Bernecky","first_name":"Carrie A","id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0893-7036"}],"citation":{"chicago":"Kaczmarek, Beata M. “Biochemical and Structural Insights into ADAR1 RNA Editing.” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/at:ista:18477\">https://doi.org/10.15479/at:ista:18477</a>.","ama":"Kaczmarek BM. Biochemical and structural insights into ADAR1 RNA editing. 2024. doi:<a href=\"https://doi.org/10.15479/at:ista:18477\">10.15479/at:ista:18477</a>","ieee":"B. M. Kaczmarek, “Biochemical and structural insights into ADAR1 RNA editing,” Institute of Science and Technology Austria, 2024.","mla":"Kaczmarek, Beata M. <i>Biochemical and Structural Insights into ADAR1 RNA Editing</i>. Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/at:ista:18477\">10.15479/at:ista:18477</a>.","apa":"Kaczmarek, B. M. (2024). <i>Biochemical and structural insights into ADAR1 RNA editing</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:18477\">https://doi.org/10.15479/at:ista:18477</a>","short":"B.M. Kaczmarek, Biochemical and Structural Insights into ADAR1 RNA Editing, Institute of Science and Technology Austria, 2024.","ista":"Kaczmarek BM. 2024. Biochemical and structural insights into ADAR1 RNA editing. Institute of Science and Technology Austria."},"has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"}],"page":"124","publication_status":"published","language":[{"iso":"eng"}],"title":"Biochemical and structural insights into ADAR1 RNA editing","type":"dissertation","date_created":"2024-10-27T07:35:13Z","alternative_title":["ISTA Thesis"],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-045-9"]},"year":"2024","doi":"10.15479/at:ista:18477","file":[{"date_updated":"2025-10-29T23:30:02Z","creator":"bkaczmar","file_size":23136626,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2024-10-29T11:56:36Z","embargo_to":"open_access","checksum":"2053294ea4d770c495e4cc501e2a218b","access_level":"closed","relation":"source_file","file_name":"20241029_PhD_thesis_BKaczmarek.docx","file_id":"18485"},{"checksum":"8ce857a4cd44b776791eaf180ac9dbb3","file_id":"18486","file_name":"20241029_PhD_thesis_BKaczmarek.pdf","embargo":"2025-10-29","relation":"main_file","access_level":"open_access","creator":"bkaczmar","date_updated":"2025-10-29T23:30:02Z","date_created":"2024-10-29T11:56:44Z","content_type":"application/pdf","file_size":11707360}],"date_published":"2024-10-29T00:00:00Z","abstract":[{"lang":"eng","text":"ADAR1 is broadly expressed across various tissues and is vital in regulating pathways\r\nassociated with innate immune responses. ADAR1 marks double-stranded RNA as \"self\"\r\nthrough its A-to-I editing activity, effectively repressing autoimmunity and maintaining\r\nimmune tolerance. This editing process has been detected at millions of sites across the\r\nhuman genome. However, the mechanism underlying ADAR1's substrate selectivity\r\nproperties remains largely unclear, with much of the current knowledge derived from\r\ncomparisons to its more extensively studied homolog, ADAR2. By studying ADAR1 in complex\r\nwith its RNA substrates and applying a combination of biochemical techniques and structural\r\nstudies using CryoEM, we aim to gain a more comprehensive understanding of the substrate\r\nselectivity characteristics of ADAR1.\r\nIn this thesis, the purification protocol for ADAR1 was successfully optimized, resulting in the\r\nfirst report in the literature to achieve high protein purity and activity. This advancement\r\nenabled the investigation of complex formation between ADAR1 and various RNA substrates,\r\nleading to the identification of optimal conditions for preparing the cryoEM sample. However,\r\ndespite comprehensive optimization of the cryo-EM conditions, the resulting data lacked the\r\ndesired quality, highlighting the need for similar rigorous optimization of the RNA substrates\r\nto facilitate structural studies of the ADAR1-RNA complex. The study was complemented by\r\nAlphaFold predictions, which provided some insights into this mechanism.\r\nMoreover, during this project I established a collaboration with a research group focused on\r\nstudying ADAR homologs. Notably ADAR homologs were identified in bivalve species, and it\r\nwas further demonstrated that ADAR and its A-to-I editing activity are upregulated in Pacific\r\noysters during infections with Ostreid herpesvirus-1—a highly infectious virus that leads to\r\nsignificant losses in oyster populations globally. I successfully purified oyster ADAR and\r\nprepared in vitro edited RNA for nanopore sequencing—a direct sequencing technology\r\ncapable of detecting modified nucleotides without the need for reverse transcription. The\r\ncollaborators initiated optimization of this nanopore-based approach. However, current\r\ntechnological limitations still constrain the reliable detection of modified nucleotides.\r\nThe project also examined the impact of RNA editing on RNA binding and filament formation\r\nby MDA5, a key cytosolic dsRNA sensor that triggers an interferon response. A primary target\r\nof ADAR1's editing activity is RNA derived from repetitive elements present in the genome,\r\nparticularly Alu elements forming double-stranded RNA. When unedited, these RNA\r\nsequences are recognized by MDA5. However, the mechanisms by which MDA5 interacts with\r\nAlu RNAs, as well as the role of A-to-I editing in influencing this binding, are still not well\r\nunderstood.\r\nThe interaction between MDA5 and Alu elements, was successfully established. This was\r\nachieved through the testing of different RNA variants and the evaluation of filament\r\nformation using binding techniques and electron microscopy imaging. This groundwork has\r\nset the conditions for further evaluation using CryoEM. Furthermore, the effects of A-to-I\r\nediting on the binding properties of MDA5 with Alu RNA were investigated. Given the recent\r\nresearch that has provided new insights into MDA5's interaction with dsRNA, it is essential to\r\nrevise the experimental setup to integrate these findings before moving forward with the\r\nCryoEM sample analysis."}],"oa_version":"Published Version","day":"29","author":[{"last_name":"Kaczmarek","full_name":"Kaczmarek, Beata M","id":"36FA4AFA-F248-11E8-B48F-1D18A9856A87","first_name":"Beata M"}]},{"status":"public","_id":"18603","corr_author":"1","scopus_import":"1","department":[{"_id":"PeJo"},{"_id":"EM-Fac"},{"_id":"RySh"}],"file_date_updated":"2024-12-03T08:56:53Z","ddc":["570"],"oa":1,"related_material":{"record":[{"relation":"research_data","status":"public","id":"18296"}]},"date_updated":"2026-04-16T12:20:34Z","month":"11","OA_place":"publisher","intvolume":"        22","publisher":"Public Library of Science","quality_controlled":"1","volume":22,"OA_type":"gold","citation":{"ieee":"O. Kim, Y. Okamoto, W. Kaufmann, N. Brose, R. Shigemoto, and P. M. Jonas, “Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons,” <i>PLoS Biology</i>, vol. 22, no. 11. Public Library of Science, 2024.","ama":"Kim O, Okamoto Y, Kaufmann W, Brose N, Shigemoto R, Jonas PM. Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons. <i>PLoS Biology</i>. 2024;22(11). doi:<a href=\"https://doi.org/10.1371/journal.pbio.3002879\">10.1371/journal.pbio.3002879</a>","chicago":"Kim, Olena, Yuji Okamoto, Walter Kaufmann, Nils Brose, Ryuichi Shigemoto, and Peter M Jonas. “Presynaptic CAMP-PKA-Mediated Potentiation Induces Reconfiguration of Synaptic Vesicle Pools and Channel-Vesicle Coupling at Hippocampal Mossy Fiber Boutons.” <i>PLoS Biology</i>. Public Library of Science, 2024. <a href=\"https://doi.org/10.1371/journal.pbio.3002879\">https://doi.org/10.1371/journal.pbio.3002879</a>.","ista":"Kim O, Okamoto Y, Kaufmann W, Brose N, Shigemoto R, Jonas PM. 2024. Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons. PLoS Biology. 22(11), e3002879.","short":"O. Kim, Y. Okamoto, W. Kaufmann, N. Brose, R. Shigemoto, P.M. Jonas, PLoS Biology 22 (2024).","apa":"Kim, O., Okamoto, Y., Kaufmann, W., Brose, N., Shigemoto, R., &#38; Jonas, P. M. (2024). Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons. <i>PLoS Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pbio.3002879\">https://doi.org/10.1371/journal.pbio.3002879</a>","mla":"Kim, Olena, et al. “Presynaptic CAMP-PKA-Mediated Potentiation Induces Reconfiguration of Synaptic Vesicle Pools and Channel-Vesicle Coupling at Hippocampal Mossy Fiber Boutons.” <i>PLoS Biology</i>, vol. 22, no. 11, e3002879, Public Library of Science, 2024, doi:<a href=\"https://doi.org/10.1371/journal.pbio.3002879\">10.1371/journal.pbio.3002879</a>."},"has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"PreCl"}],"article_processing_charge":"Yes","ec_funded":1,"publication_status":"published","DOAJ_listed":"1","article_type":"original","title":"Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons","type":"journal_article","project":[{"call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse"},{"call_identifier":"FWF","_id":"25C5A090-B435-11E9-9278-68D0E5697425","name":"Synaptic communication in neuronal microcircuits","grant_number":"Z00312"},{"_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","name":"Mechanisms of GABA release in hippocampal circuits","grant_number":"P36232"},{"name":"Structural & functional basis of presynaptic plasticity","grant_number":"I06166","_id":"b1b85715-d554-11ed-a5ad-84a07fc9f18e"},{"_id":"25C3DBB6-B435-11E9-9278-68D0E5697425","name":"Zellkommunikation in Gesundheit und Krankheit","grant_number":"W01205","call_identifier":"FWF"},{"call_identifier":"FWF","_id":"3AC91DDA-15DF-11EA-824D-93A3E7B544D1","name":"FWF Open Access Fund"}],"isi":1,"language":[{"iso":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-12-01T23:01:54Z","acknowledgement":"We thank Carolina Borges-Merjane, Jing-Jing Chen, Katharina Lichter, and Samuel Young for critically reading the manuscript; the Electron Microscopy Facility of ISTA, in particular Vanessa Zheden, for extensive support, advice, and experimental assistance; the Preclinical Facility of ISTA, in particular Victoria Wimmer and Michael Schunn, for experimental assistance; Florian Marr and Christina Altmutter for technical support; Alois Schlögl for help with analysis; and Eleftheria Kralli-Beller for manuscript editing. We also thank Cordelia Imig for providing Munc13-1cKO-Munc13-2/3(−/−) mutant mice. Part of the work has been published in O.K.’s thesis in partial fulfillment of the requirements for the degree of Doctor of Philosophy.\r\nThis project received funding from the European Research Council and European Union’s Horizon 2020 research and innovation programme (ERC 692692 to P.J.; https://cordis.europa.eu/project/id/692692/de) and from the Fond zur Förderung der Wissenschaftlichen Forschung (Z312-B27 Wittgenstein award to P.J., https://www.fwf.ac.at/en/funding/portfolio/projects/fwf-wittgenstein-award; W1205-B09 and P36232-B to P.J., https://www.fwf.ac.at/en/funding; I6166-B to R.S.; https://www.fwf.ac.at/en/funding). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","doi":"10.1371/journal.pbio.3002879","year":"2024","article_number":"e3002879","publication_identifier":{"issn":["1544-9173"],"eissn":["1545-7885"]},"publication":"PLoS Biology","date_published":"2024-11-18T00:00:00Z","file":[{"access_level":"open_access","relation":"main_file","file_id":"18608","file_name":"2024_PloSBio_Kim.pdf","checksum":"7de2dcb50deb65dde05c80082bb85a82","file_size":3057631,"date_created":"2024-12-03T08:56:53Z","content_type":"application/pdf","success":1,"date_updated":"2024-12-03T08:56:53Z","creator":"dernst"}],"external_id":{"isi":["001358568700003"],"pmid":["39556620"]},"abstract":[{"lang":"eng","text":"It is widely believed that information storage in neuronal circuits involves nanoscopic structural changes at synapses, resulting in the formation of synaptic engrams. However, direct evidence for this hypothesis is lacking. To test this conjecture, we combined chemical potentiation, functional analysis by paired pre-postsynaptic recordings, and structural analysis by electron microscopy (EM) and freeze-fracture replica labeling (FRL) at the rodent hippocampal mossy fiber synapse, a key synapse in the trisynaptic circuit of the hippocampus. Biophysical analysis of synaptic transmission revealed that forskolin-induced chemical potentiation increased the readily releasable vesicle pool size and vesicular release probability by 146% and 49%, respectively. Structural analysis of mossy fiber synapses by EM and FRL demonstrated an increase in the number of vesicles close to the plasma membrane and the number of clusters of the priming protein Munc13-1, indicating an increase in the number of both docked and primed vesicles. Furthermore, FRL analysis revealed a significant reduction of the distance between Munc13-1 and CaV2.1 Ca2+ channels, suggesting reconfiguration of the channel-vesicle coupling nanotopography. Our results indicate that presynaptic plasticity is associated with structural reorganization of active zones. We propose that changes in potential nanoscopic organization at synaptic vesicle release sites may be correlates of learning and memory at a plastic central synapse."}],"pmid":1,"oa_version":"Published Version","issue":"11","author":[{"first_name":"Olena","id":"3F8ABDDA-F248-11E8-B48F-1D18A9856A87","full_name":"Kim, Olena","last_name":"Kim","orcid":"0000-0003-2344-1039"},{"last_name":"Okamoto","full_name":"Okamoto, Yuji","id":"3337E116-F248-11E8-B48F-1D18A9856A87","first_name":"Yuji","orcid":"0000-0003-0408-6094"},{"orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","last_name":"Kaufmann","first_name":"Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Brose, Nils","last_name":"Brose","first_name":"Nils"},{"last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi","orcid":"0000-0001-8761-9444"},{"orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M","last_name":"Jonas","first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"APC_amount":"6248,82 EUR","day":"18"},{"citation":{"ieee":"J. Datler, “Elucidating the structural determinants of the poxvirus core using multi-modal cryo-EM,” Institute of Science and Technology Austria, 2024.","ama":"Datler J. Elucidating the structural determinants of the poxvirus core using multi-modal cryo-EM. 2024. doi:<a href=\"https://doi.org/10.15479/at:ista:18766\">10.15479/at:ista:18766</a>","chicago":"Datler, Julia. “Elucidating the Structural Determinants of the Poxvirus Core Using Multi-Modal Cryo-EM.” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/at:ista:18766\">https://doi.org/10.15479/at:ista:18766</a>.","ista":"Datler J. 2024. Elucidating the structural determinants of the poxvirus core using multi-modal cryo-EM. Institute of Science and Technology Austria.","short":"J. Datler, Elucidating the Structural Determinants of the Poxvirus Core Using Multi-Modal Cryo-EM, Institute of Science and Technology Austria, 2024.","apa":"Datler, J. (2024). <i>Elucidating the structural determinants of the poxvirus core using multi-modal cryo-EM</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:18766\">https://doi.org/10.15479/at:ista:18766</a>","mla":"Datler, Julia. <i>Elucidating the Structural Determinants of the Poxvirus Core Using Multi-Modal Cryo-EM</i>. Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/at:ista:18766\">10.15479/at:ista:18766</a>."},"has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)"},"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"article_processing_charge":"No","page":"106","month":"12","OA_place":"publisher","supervisor":[{"orcid":"0000-0003-4790-8078","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM","last_name":"Schur","full_name":"Schur, Florian KM"}],"publisher":"Institute of Science and Technology Austria","oa":1,"related_material":{"record":[{"id":"12334","relation":"part_of_dissertation","status":"public"},{"id":"14979","status":"public","relation":"part_of_dissertation"}]},"date_updated":"2026-04-07T12:59:44Z","_id":"18766","corr_author":"1","status":"public","department":[{"_id":"GradSch"},{"_id":"FlSc"}],"file_date_updated":"2025-01-07T12:15:14Z","ddc":["570"],"degree_awarded":"PhD","oa_version":"Published Version","day":"30","author":[{"id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87","first_name":"Julia","last_name":"Datler","full_name":"Datler, Julia","orcid":"0000-0002-3616-8580"}],"file":[{"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2025-01-07T12:15:11Z","file_size":38814932,"creator":"jstanger","date_updated":"2025-01-07T12:15:11Z","file_name":"PhD_thesis_Julia_Datler.docx","file_id":"18769","relation":"source_file","access_level":"closed","checksum":"3e51cab327c754045c3d29c1a50cc9a9"},{"creator":"jstanger","date_updated":"2025-01-07T12:15:14Z","success":1,"content_type":"application/pdf","date_created":"2025-01-07T12:15:14Z","file_size":12044865,"checksum":"22fabe5b97950bf852212f6edb555173","file_name":"PhD_thesis_Julia_Datler.pdf","file_id":"18770","access_level":"open_access","relation":"main_file"}],"date_published":"2024-12-30T00:00:00Z","abstract":[{"lang":"eng","text":"Poxviruses are large pleomorphic double-stranded DNA viruses that include well known members such as variola virus, the causative agent of smallpox, Mpox virus, as well as Vaccinia virus (VACV), which serves as a vaccination strain for formerly mentioned viruses. VACV is a valuable model for studying large pleomorphic DNA viruses in general and poxviruses specifically, as many features, such as core morphology and structural proteins, are well conserved within this family. Despite decades of research, our understanding of the structural components and proteins that comprise the poxvirus core in mature virions remains limited. Although major core proteins were identified via indirect experimental evidence, the core's complexity, with its large size, structure and number of involved proteins, has hindered efforts to achieve high-resolution insights and to define the roles of the individual proteins. The specific protein composition of the core's individual layers, including the palisade layer and the inner core wall, has remained unclear. In this study, we have merged multiple approaches, including single particle cryo electron microscopy of purified virus cores, cryo-electron tomography and subtomogram averaging of mature virions and molecular modeling to elucidate the structural determinants of the VACV core. Due to the lack of experimentally derived structures, either in situ or reconstituted in vitro, we used Alphafold to predict models of the putative major core protein candidates, A10, 23k, A3, A4, and L4. Our results show that the VACV core is composed of several layers with varying local symmetries, forming more intricate interactions than observed previously. This allowed us to identify several molecular building blocks forming the viral core lattice. In particular, we identified trimers of protein A10 as a major core structure that forms the palisade layer of the viral core. Additionally, we revealed that six petals of a flower shaped core pore within the core wall are composed of A10 trimers. Furthermore, we obtained a cryo-EM density for the inner core wall that could potentially accommodate an A3 dimer. Integrating descriptions of protein interactions from previous studies enabled us to provide a detailed structural model of the poxvirus core wall, and our findings indicate that the interactions within A10 trimers are likely consistent across orthopox- and parapoxviruses. This combined application of cryo-SPA and cryo-ET can help overcome obstacles in studying complex virus structures in the future, including their key assembly proteins, interactions, and the formation into a core lattice. Our work provides important fundamental new insights into poxvirus core architecture, also considering the recent re-emergence of poxviruses."}],"alternative_title":["ISTA thesis"],"date_created":"2025-01-07T10:23:12Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-049-7"]},"year":"2024","acknowledgement":"This work was funded by the Austrian Science Fund (FWF) grant P31445 and ISTA. I\r\nwould like to express my gratitude to the Scientific Service Units, particularly the Lab\r\nSupport Facility, the Scientific Computing Facility and the Electron Microscopy Facility\r\nfor their tremendous support. I want to especially thank Alois for assisting me with the\r\ninstallation of countless new software and for troubleshooting cluster issues. A special\r\nthanks goes to Valentin for his outstanding support in cryo-EM data acquisition and\r\nhis ongoing help in improving the process to ensure that I obtained the best possible\r\ndata from my sample.","doi":"10.15479/at:ista:18766","keyword":["cryo-EM","cryo-ET","cryo-SPA","Structural Virology","Poxvirus","Vaccinia Virus","Structural Biology"],"publication_status":"published","language":[{"iso":"eng"}],"title":"Elucidating the structural determinants of the poxvirus core using multi-modal cryo-EM","type":"dissertation","project":[{"call_identifier":"FWF","_id":"26736D6A-B435-11E9-9278-68D0E5697425","grant_number":"P31445","name":"Structural conservation and diversity in retroviral capsid"}]},{"issue":"5","pmid":1,"oa_version":"Published Version","day":"06","PlanS_conform":"1","author":[{"full_name":"Chen, JingJing","last_name":"Chen","first_name":"JingJing","id":"2C4E65C8-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kaufmann, Walter","last_name":"Kaufmann","first_name":"Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9735-5315"},{"full_name":"Chen, Chong","last_name":"Chen","first_name":"Chong","id":"3DFD581A-F248-11E8-B48F-1D18A9856A87"},{"id":"32A73F6C-F248-11E8-B48F-1D18A9856A87","first_name":"Itaru","last_name":"Arai","full_name":"Arai, Itaru"},{"orcid":"0000-0003-2344-1039","first_name":"Olena","id":"3F8ABDDA-F248-11E8-B48F-1D18A9856A87","full_name":"Kim, Olena","last_name":"Kim"},{"orcid":"0000-0001-8761-9444","last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi"},{"first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Jonas, Peter M","last_name":"Jonas","orcid":"0000-0001-5001-4804"}],"external_id":{"isi":["001202925700001"],"pmid":["38215739"]},"date_published":"2024-03-06T00:00:00Z","file":[{"file_size":8192355,"content_type":"application/pdf","date_created":"2025-04-23T14:02:08Z","success":1,"date_updated":"2025-04-23T14:02:08Z","creator":"dernst","relation":"main_file","access_level":"open_access","file_id":"19614","file_name":"2024_Neuron_Chen.pdf","checksum":"30098b4f0209556ddfb3540a23d07ca5"}],"publication":"Neuron","abstract":[{"text":"The coupling between Ca2+ channels and release sensors is a key factor defining the signaling properties of a synapse. However, the coupling nanotopography at many synapses remains unknown, and it is unclear how it changes during development. To address these questions, we examined coupling at the cerebellar inhibitory basket cell (BC)-Purkinje cell (PC) synapse. Biophysical analysis of transmission by paired recording and intracellular pipette perfusion revealed that the effects of exogenous Ca2+ chelators decreased during development, despite constant reliance of release on P/Q-type Ca2+ channels. Structural analysis by freeze-fracture replica labeling (FRL) and transmission electron microscopy (EM) indicated that presynaptic P/Q-type Ca2+ channels formed nanoclusters throughout development, whereas docked vesicles were only clustered at later developmental stages. Modeling suggested a developmental transformation from a more random to a more clustered coupling nanotopography. Thus, presynaptic signaling developmentally approaches a point-to-point configuration, optimizing speed, reliability, and energy efficiency of synaptic transmission.","lang":"eng"}],"date_created":"2024-01-21T23:00:56Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"eissn":["1097-4199"],"issn":["0896-6273"]},"year":"2024","acknowledgement":"We thank Drs. David DiGregorio and Erwin Neher for critically reading an earlier version of the manuscript, Ralf Schneggenburger for helpful discussions, Benjamin Suter and Katharina Lichter for support with image analysis, Chris Wojtan for advice on numerical solution of partial differential equations, Maria Reva for help with Ripley analysis, Alois Schlögl for programming, and Akari Hagiwara and Toshihisa Ohtsuka for anti-ELKS antibody. We are grateful to Florian Marr, Christina Altmutter, and Vanessa Zheden for excellent technical assistance and to Eleftheria Kralli-Beller for manuscript editing. This research was supported by the Scientific Services Units (SSUs) of ISTA (Electron Microscopy Facility, Preclinical Facility, and Machine Shop). The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 692692), the Fonds zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award; P 36232-B), all to P.J., and a DOC fellowship of the Austrian Academy of Sciences to J.-J.C.","doi":"10.1016/j.neuron.2023.12.002","article_type":"original","publication_status":"published","isi":1,"language":[{"iso":"eng"}],"type":"journal_article","title":"Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse","project":[{"_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","grant_number":"692692","call_identifier":"H2020"},{"grant_number":"Z00312","name":"Synaptic communication in neuronal microcircuits","_id":"25C5A090-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","grant_number":"P36232","name":"Mechanisms of GABA release in hippocampal circuits"},{"name":"Development of nanodomain coupling between Ca2+ channels and release sensors at a central inhibitory synapse","grant_number":"25383","_id":"26B66A3E-B435-11E9-9278-68D0E5697425"}],"citation":{"mla":"Chen, JingJing, et al. “Developmental Transformation of Ca2+ Channel-Vesicle Nanotopography at a Central GABAergic Synapse.” <i>Neuron</i>, vol. 112, no. 5, Elsevier, 2024, p. 755–771.e9, doi:<a href=\"https://doi.org/10.1016/j.neuron.2023.12.002\">10.1016/j.neuron.2023.12.002</a>.","apa":"Chen, J., Kaufmann, W., Chen, C., Arai,  itaru, Kim, O., Shigemoto, R., &#38; Jonas, P. M. (2024). Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2023.12.002\">https://doi.org/10.1016/j.neuron.2023.12.002</a>","ista":"Chen J, Kaufmann W, Chen C, Arai  itaru, Kim O, Shigemoto R, Jonas PM. 2024. Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse. Neuron. 112(5), 755–771.e9.","short":"J. Chen, W. Kaufmann, C. Chen,  itaru Arai, O. Kim, R. Shigemoto, P.M. Jonas, Neuron 112 (2024) 755–771.e9.","ama":"Chen J, Kaufmann W, Chen C, et al. Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse. <i>Neuron</i>. 2024;112(5):755-771.e9. doi:<a href=\"https://doi.org/10.1016/j.neuron.2023.12.002\">10.1016/j.neuron.2023.12.002</a>","chicago":"Chen, JingJing, Walter Kaufmann, Chong Chen, itaru Arai, Olena Kim, Ryuichi Shigemoto, and Peter M Jonas. “Developmental Transformation of Ca2+ Channel-Vesicle Nanotopography at a Central GABAergic Synapse.” <i>Neuron</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.neuron.2023.12.002\">https://doi.org/10.1016/j.neuron.2023.12.002</a>.","ieee":"J. Chen <i>et al.</i>, “Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse,” <i>Neuron</i>, vol. 112, no. 5. Elsevier, p. 755–771.e9, 2024."},"has_accepted_license":"1","volume":112,"OA_type":"hybrid","quality_controlled":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"Yes (via OA deal)","ec_funded":1,"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"PreCl"},{"_id":"M-Shop"}],"page":"755-771.e9","month":"03","OA_place":"publisher","publisher":"Elsevier","intvolume":"       112","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"15101"}],"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/synapses-brought-to-the-point/","description":"News on ISTA Website"}]},"oa":1,"date_updated":"2026-06-21T22:30:29Z","_id":"14843","corr_author":"1","status":"public","file_date_updated":"2025-04-23T14:02:08Z","department":[{"_id":"PeJo"},{"_id":"EM-Fac"},{"_id":"RySh"}],"ddc":["570"],"scopus_import":"1"},{"external_id":{"pmid":["38370025"],"isi":["001138880800005"]},"date_published":"2024-02-01T00:00:00Z","file":[{"date_created":"2024-07-16T12:12:43Z","content_type":"application/pdf","success":1,"file_size":9897883,"creator":"dernst","date_updated":"2024-07-16T12:12:43Z","file_id":"17267","file_name":"2024_NaturePhysics_CaballeroMancebo.pdf","access_level":"open_access","relation":"main_file","checksum":"7891ebe7c900ae47469ab127031dd1ec"}],"publication":"Nature Physics","abstract":[{"lang":"eng","text":"Contraction and flow of the actin cell cortex have emerged as a common principle by which cells reorganize their cytoplasm and take shape. However, how these cortical flows interact with adjacent cytoplasmic components, changing their form and localization, and how this affects cytoplasmic organization and cell shape remains unclear. Here we show that in ascidian oocytes, the cooperative activities of cortical actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive oocyte cytoplasmic reorganization and shape changes following fertilization. We show that vegetal-directed cortical actomyosin flows, established upon oocyte fertilization, lead to both the accumulation of cortical actin at the vegetal pole of the zygote and compression and local buckling of the adjacent elastic solid-like myoplasm layer due to friction forces generated at their interface. Once cortical flows have ceased, the multiple myoplasm buckles resolve into one larger buckle, which again drives the formation of the contraction pole—a protuberance of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings reveal a mechanism where cortical actomyosin network flows determine cytoplasmic reorganization and cell shape by deforming adjacent cytoplasmic components through friction forces."}],"oa_version":"Published Version","pmid":1,"day":"01","author":[{"orcid":"0000-0002-5223-3346","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","first_name":"Silvia","last_name":"Caballero Mancebo","full_name":"Caballero Mancebo, Silvia"},{"first_name":"Rushikesh","full_name":"Shinde, Rushikesh","last_name":"Shinde"},{"full_name":"Bolger-Munro, Madison","last_name":"Bolger-Munro","first_name":"Madison","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","orcid":"0000-0002-8176-4824"},{"orcid":"0000-0002-3415-4628","id":"3F920B30-F248-11E8-B48F-1D18A9856A87","first_name":"Matilda","last_name":"Peruzzo","full_name":"Peruzzo, Matilda"},{"id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","first_name":"Gregory","last_name":"Szep","full_name":"Szep, Gregory"},{"id":"2705C766-9FE2-11EA-B224-C6773DDC885E","first_name":"Irene","last_name":"Steccari","full_name":"Steccari, Irene"},{"full_name":"Labrousse Arias, David","last_name":"Labrousse Arias","first_name":"David","id":"CD573DF4-9ED3-11E9-9D77-3223E6697425"},{"last_name":"Zheden","full_name":"Zheden, Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","orcid":"0000-0002-9438-4783"},{"full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609"},{"full_name":"Callan-Jones, Andrew","last_name":"Callan-Jones","first_name":"Andrew"},{"first_name":"Raphaël","full_name":"Voituriez, Raphaël","last_name":"Voituriez"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"article_type":"original","publication_status":"published","isi":1,"language":[{"iso":"eng"}],"project":[{"_id":"2646861A-B435-11E9-9278-68D0E5697425","grant_number":"I03601","name":"Control of embryonic cleavage pattern","call_identifier":"FWF"}],"type":"journal_article","title":"Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization","date_created":"2024-01-21T23:00:57Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"year":"2024","doi":"10.1038/s41567-023-02302-1","acknowledgement":"We would like to thank A. McDougall, E. Hannezo and the Heisenberg lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Electron Microscopy Facility, Imaging and Optics Facility and the Nanofabrication Facility. This work was supported by a Joint Project Grant from the FWF (I 3601-B27).","month":"02","publisher":"Springer Nature","intvolume":"        20","has_accepted_license":"1","citation":{"mla":"Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>, vol. 20, Springer Nature, 2024, pp. 310–21, doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>.","apa":"Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G., Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>","short":"S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I. Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez, C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321.","ista":"Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.","chicago":"Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>.","ama":"Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. 2024;20:310-321. doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>","ieee":"S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature Physics</i>, vol. 20. Springer Nature, pp. 310–321, 2024."},"volume":20,"quality_controlled":"1","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"NanoFab"}],"article_processing_charge":"Yes (in subscription journal)","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"page":"310-321","_id":"14846","corr_author":"1","status":"public","ddc":["530"],"file_date_updated":"2024-07-16T12:12:43Z","department":[{"_id":"CaHe"},{"_id":"JoFi"},{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"scopus_import":"1","related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/","description":"News on ISTA Website"}]},"oa":1,"date_updated":"2025-09-04T11:48:28Z"},{"volume":31,"OA_type":"hybrid","quality_controlled":"1","has_accepted_license":"1","citation":{"ama":"Datler J, Hansen J, Thader A, et al. Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. <i>Nature Structural &#38; Molecular Biology</i>. 2024;31:1114-1123. doi:<a href=\"https://doi.org/10.1038/s41594-023-01201-6\">10.1038/s41594-023-01201-6</a>","chicago":"Datler, Julia, Jesse Hansen, Andreas Thader, Alois Schlögl, Lukas W Bauer, Victor-Valentin Hodirnau, and Florian KM Schur. “Multi-Modal Cryo-EM Reveals Trimers of Protein A10 to Form the Palisade Layer in Poxvirus Cores.” <i>Nature Structural &#38; Molecular Biology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41594-023-01201-6\">https://doi.org/10.1038/s41594-023-01201-6</a>.","ieee":"J. Datler <i>et al.</i>, “Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores,” <i>Nature Structural &#38; Molecular Biology</i>, vol. 31. Springer Nature, pp. 1114–1123, 2024.","apa":"Datler, J., Hansen, J., Thader, A., Schlögl, A., Bauer, L. W., Hodirnau, V.-V., &#38; Schur, F. K. (2024). Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. <i>Nature Structural &#38; Molecular Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41594-023-01201-6\">https://doi.org/10.1038/s41594-023-01201-6</a>","mla":"Datler, Julia, et al. “Multi-Modal Cryo-EM Reveals Trimers of Protein A10 to Form the Palisade Layer in Poxvirus Cores.” <i>Nature Structural &#38; Molecular Biology</i>, vol. 31, Springer Nature, 2024, pp. 1114–23, doi:<a href=\"https://doi.org/10.1038/s41594-023-01201-6\">10.1038/s41594-023-01201-6</a>.","ista":"Datler J, Hansen J, Thader A, Schlögl A, Bauer LW, Hodirnau V-V, Schur FK. 2024. Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. Nature Structural &#38; Molecular Biology. 31, 1114–1123.","short":"J. Datler, J. Hansen, A. Thader, A. Schlögl, L.W. Bauer, V.-V. Hodirnau, F.K. Schur, Nature Structural &#38; Molecular Biology 31 (2024) 1114–1123."},"page":"1114-1123","article_processing_charge":"Yes (in subscription journal)","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"OA_place":"publisher","month":"07","intvolume":"        31","publisher":"Springer Nature","oa":1,"related_material":{"record":[{"id":"18766","status":"public","relation":"dissertation_contains"}],"link":[{"description":"News on ISTA Website","url":"https://ista.ac.at/en/news/down-to-the-core-of-poxviruses/","relation":"press_release"}]},"date_updated":"2026-04-07T12:59:44Z","status":"public","corr_author":"1","_id":"14979","scopus_import":"1","ddc":["570"],"department":[{"_id":"FlSc"},{"_id":"ScienComp"},{"_id":"EM-Fac"}],"file_date_updated":"2024-07-22T11:27:22Z","oa_version":"Published Version","pmid":1,"author":[{"orcid":"0000-0002-3616-8580","first_name":"Julia","id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87","full_name":"Datler, Julia","last_name":"Datler"},{"orcid":"0000-0001-7967-2085","id":"1063c618-6f9b-11ec-9123-f912fccded63","first_name":"Jesse","last_name":"Hansen","full_name":"Hansen, Jesse"},{"last_name":"Thader","full_name":"Thader, Andreas","id":"3A18A7B8-F248-11E8-B48F-1D18A9856A87","first_name":"Andreas"},{"last_name":"Schlögl","full_name":"Schlögl, Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","first_name":"Alois","orcid":"0000-0002-5621-8100"},{"last_name":"Bauer","full_name":"Bauer, Lukas W","id":"0c894dcf-897b-11ed-a09c-8186353224b0","first_name":"Lukas W"},{"full_name":"Hodirnau, Victor-Valentin","last_name":"Hodirnau","first_name":"Victor-Valentin","id":"3661B498-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3904-947X"},{"orcid":"0000-0003-4790-8078","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM","last_name":"Schur","full_name":"Schur, Florian KM"}],"APC_amount":"11700 EUR","day":"01","publication":"Nature Structural & Molecular Biology","date_published":"2024-07-01T00:00:00Z","external_id":{"pmid":["38316877"],"isi":["001158144600002"]},"file":[{"file_id":"17307","file_name":"2024_NatureStrucBio_Datler.pdf","access_level":"open_access","relation":"main_file","checksum":"bda7bf65d81455480efaed8ca293b0db","date_created":"2024-07-22T11:27:22Z","content_type":"application/pdf","success":1,"file_size":17485494,"creator":"dernst","date_updated":"2024-07-22T11:27:22Z"}],"abstract":[{"text":"Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remained sparse. While major core proteins have been annotated via indirect experimental evidence, their structures have remained elusive and they could not be assigned to individual core features. Hence, which proteins constitute which layers of the core, such as the palisade layer and the inner core wall, has remained enigmatic. Here we show, using a multi-modal cryo-electron microscopy (cryo-EM) approach in combination with AlphaFold molecular modeling, that trimers formed by the cleavage product of VACV protein A10 are the key component of the palisade layer. This allows us to place previously obtained descriptions of protein interactions within the core wall into perspective and to provide a detailed model of poxvirus core architecture. Importantly, we show that interactions within A10 trimers are likely generalizable over members of orthopox- and parapoxviruses.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-02-12T09:59:45Z","keyword":["Molecular Biology","Structural Biology"],"acknowledgement":"We thank A. Bergthaler (Research Center for Molecular Medicine of the Austrian Academy of Sciences) for providing VACV WR. We thank A. Nicholas and his team at the ISTA proteomics facility, and S. Elefante at the ISTA Scientific Computing facility for their support. We also thank F. Fäßler, D. Porley, T. Muthspiel and other members of the Schur group for support and helpful discussions. We also thank D. Castaño-Díez for support with Dynamo. We thank D. Farrell for his help optimizing the Rosetta protocol to refine the atomic model into the cryo-EM map with symmetry.\r\n\r\nF.K.M.S. acknowledges support from ISTA and EMBO. F.K.M.S. also received support from the Austrian Science Fund (FWF) grant P31445. This publication has been made possible in part by CZI grant DAF2021-234754 and grant https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (funder https://doi.org/10.13039/100014989) awarded to F.K.M.S.\r\n\r\nThis research was also supported by the Scientific Service Units (SSUs) of ISTA through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), and the Electron Microscopy Facility (EMF). We also acknowledge the use of COSMIC45 and Colabfold46.","doi":"10.1038/s41594-023-01201-6","publication_identifier":{"issn":["1545-9993"],"eissn":["1545-9985"]},"year":"2024","publication_status":"published","article_type":"original","project":[{"call_identifier":"FWF","name":"Structural conservation and diversity in retroviral capsid","grant_number":"P31445","_id":"26736D6A-B435-11E9-9278-68D0E5697425"}],"type":"journal_article","title":"Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores","language":[{"iso":"eng"}],"isi":1},{"article_processing_charge":"Yes (in subscription journal)","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"PreCl"},{"_id":"EM-Fac"}],"ec_funded":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)"},"quality_controlled":"1","OA_type":"hybrid","volume":121,"has_accepted_license":"1","citation":{"ieee":"P. Koppensteiner <i>et al.</i>, “GABAB receptors induce phasic release from medial habenula terminals through activity-dependent recruitment of release-ready vesicles,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 8. National Academy of Sciences, 2024.","chicago":"Koppensteiner, Peter, Pradeep Bhandari, Cihan Önal, Carolina Borges Merjane, Elodie Le Monnier, Utsa Roy, Yukihiro Nakamura, et al. “GABAB Receptors Induce Phasic Release from Medial Habenula Terminals through Activity-Dependent Recruitment of Release-Ready Vesicles.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2024. <a href=\"https://doi.org/10.1073/pnas.2301449121\">https://doi.org/10.1073/pnas.2301449121</a>.","ama":"Koppensteiner P, Bhandari P, Önal C, et al. GABAB receptors induce phasic release from medial habenula terminals through activity-dependent recruitment of release-ready vesicles. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2024;121(8). doi:<a href=\"https://doi.org/10.1073/pnas.2301449121\">10.1073/pnas.2301449121</a>","short":"P. Koppensteiner, P. Bhandari, C. Önal, C. Borges Merjane, E. Le Monnier, U. Roy, Y. Nakamura, T. Sadakata, M. Sanbo, M. Hirabayashi, J. Rhee, N. Brose, P.M. Jonas, R. Shigemoto, Proceedings of the National Academy of Sciences of the United States of America 121 (2024).","ista":"Koppensteiner P, Bhandari P, Önal C, Borges Merjane C, Le Monnier E, Roy U, Nakamura Y, Sadakata T, Sanbo M, Hirabayashi M, Rhee J, Brose N, Jonas PM, Shigemoto R. 2024. GABAB receptors induce phasic release from medial habenula terminals through activity-dependent recruitment of release-ready vesicles. Proceedings of the National Academy of Sciences of the United States of America. 121(8), e2301449121.","apa":"Koppensteiner, P., Bhandari, P., Önal, C., Borges Merjane, C., Le Monnier, E., Roy, U., … Shigemoto, R. (2024). GABAB receptors induce phasic release from medial habenula terminals through activity-dependent recruitment of release-ready vesicles. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2301449121\">https://doi.org/10.1073/pnas.2301449121</a>","mla":"Koppensteiner, Peter, et al. “GABAB Receptors Induce Phasic Release from Medial Habenula Terminals through Activity-Dependent Recruitment of Release-Ready Vesicles.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 8, e2301449121, National Academy of Sciences, 2024, doi:<a href=\"https://doi.org/10.1073/pnas.2301449121\">10.1073/pnas.2301449121</a>."},"intvolume":"       121","publisher":"National Academy of Sciences","OA_place":"publisher","month":"02","date_updated":"2026-06-21T22:30:30Z","related_material":{"record":[{"id":"13173","relation":"research_data","status":"public"},{"status":"public","relation":"dissertation_contains","id":"19271"}],"link":[{"relation":"press_release","description":"News on ISTA Website","url":"https://ista.ac.at/en/news/neuronal-insights-flash-and-freeze-fracture/"}]},"oa":1,"scopus_import":"1","ddc":["570"],"department":[{"_id":"RySh"},{"_id":"PeJo"}],"file_date_updated":"2024-03-12T13:42:42Z","status":"public","_id":"15084","corr_author":"1","author":[{"orcid":"0000-0002-3509-1948","last_name":"Koppensteiner","full_name":"Koppensteiner, Peter","id":"3B8B25A8-F248-11E8-B48F-1D18A9856A87","first_name":"Peter"},{"orcid":"0000-0003-0863-4481","last_name":"Bhandari","full_name":"Bhandari, Pradeep","id":"45EDD1BC-F248-11E8-B48F-1D18A9856A87","first_name":"Pradeep"},{"orcid":"0000-0002-2771-2011","last_name":"Önal","full_name":"Önal, Hüseyin C","id":"4659D740-F248-11E8-B48F-1D18A9856A87","first_name":"Hüseyin C"},{"orcid":"0000-0003-0005-401X","last_name":"Borges Merjane","full_name":"Borges Merjane, Carolina","id":"4305C450-F248-11E8-B48F-1D18A9856A87","first_name":"Carolina"},{"id":"3B59276A-F248-11E8-B48F-1D18A9856A87","first_name":"Elodie","last_name":"Le Monnier","full_name":"Le Monnier, Elodie"},{"last_name":"Roy","full_name":"Roy, Utsa","id":"4d26cf11-5355-11ee-ae5a-eb05e255b9b2","first_name":"Utsa"},{"first_name":"Yukihiro","full_name":"Nakamura, Yukihiro","last_name":"Nakamura"},{"first_name":"Tetsushi","last_name":"Sadakata","full_name":"Sadakata, Tetsushi"},{"first_name":"Makoto","last_name":"Sanbo","full_name":"Sanbo, Makoto"},{"first_name":"Masumi","last_name":"Hirabayashi","full_name":"Hirabayashi, Masumi"},{"first_name":"JeongSeop","last_name":"Rhee","full_name":"Rhee, JeongSeop"},{"full_name":"Brose, Nils","last_name":"Brose","first_name":"Nils"},{"first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Jonas, Peter M","last_name":"Jonas","orcid":"0000-0001-5001-4804"},{"first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","orcid":"0000-0001-8761-9444"}],"APC_amount":"5887,8 EUR","day":"20","pmid":1,"oa_version":"Published Version","issue":"8","abstract":[{"text":"GABAB receptor (GBR) activation inhibits neurotransmitter release in axon terminals in the brain, except in medial habenula (MHb) terminals, which show robust potentiation. However, mechanisms underlying this enigmatic potentiation remain elusive. Here, we report that GBR activation on MHb terminals induces an activity-dependent transition from a facilitating, tonic to a depressing, phasic neurotransmitter release mode. This transition is accompanied by a 4.1-fold increase in readily releasable vesicle pool (RRP) size and a 3.5-fold increase of docked synaptic vesicles (SVs) at the presynaptic active zone (AZ). Strikingly, the depressing phasic release exhibits looser coupling distance than the tonic release. Furthermore, the tonic and phasic release are selectively affected by deletion of synaptoporin (SPO) and Ca\r\n            <jats:sup>2+</jats:sup>\r\n            -dependent activator protein for secretion 2 (CAPS2), respectively. SPO modulates augmentation, the short-term plasticity associated with tonic release, and CAPS2 retains the increased RRP for initial responses in phasic response trains. The cytosolic protein CAPS2 showed a SV-associated distribution similar to the vesicular transmembrane protein SPO, and they were colocalized in the same terminals. We developed the “Flash and Freeze-fracture” method, and revealed the release of SPO-associated vesicles in both tonic and phasic modes and activity-dependent recruitment of CAPS2 to the AZ during phasic release, which lasted several minutes. Overall, these results indicate that GBR activation translocates CAPS2 to the AZ along with the fusion of CAPS2-associated SVs, contributing to persistency of the RRP increase. Thus, we identified structural and molecular mechanisms underlying tonic and phasic neurotransmitter release and their transition by GBR activation in MHb terminals.","lang":"eng"}],"publication":"Proceedings of the National Academy of Sciences of the United States of America","file":[{"file_name":"2024_PNAS_Koppensteiner.pdf","file_id":"15110","relation":"main_file","access_level":"open_access","checksum":"b25b2a057c266ff317a48b0d54d6fc8a","success":1,"content_type":"application/pdf","date_created":"2024-03-12T13:42:42Z","file_size":13648221,"creator":"dernst","date_updated":"2024-03-12T13:42:42Z"}],"external_id":{"pmid":["38346189"],"isi":["001208567300006"]},"date_published":"2024-02-20T00:00:00Z","acknowledgement":"We thank Erwin Neher and Ipe Ninan for critical comments on the manuscript. This project has received funding from the European Research Council (ERC) and European Commission, under the European Union’s Horizon 2020 research and innovation program (ERC grant agreement no. 694539 to R.S. and the Marie Skłodowska-Curie grant agreement no. 665385 to C.Ö.). This study was supported by the Cooperative Study Program of Center for Animal Resources and Collaborative Study of NINS. We thank Kohgaku Eguchi for statistical analysis, Yu Kasugai for additional EM imaging, Robert Beattie for the design of the slice recovery chamber for Flash and Freeze experiments, Todor Asenov from the ISTA machine shop for custom part preparations for high-pressure freezing, the ISTA preclinical facility for animal caretaking, and the ISTA EM facilities for technical support.","doi":"10.1073/pnas.2301449121","year":"2024","article_number":"e2301449121","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-03-05T09:23:55Z","project":[{"_id":"25CA28EA-B435-11E9-9278-68D0E5697425","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour","grant_number":"694539","call_identifier":"H2020"},{"call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","name":"International IST Doctoral Program"}],"type":"journal_article","title":"GABAB receptors induce phasic release from medial habenula terminals through activity-dependent recruitment of release-ready vesicles","isi":1,"language":[{"iso":"eng"}],"publication_status":"published","article_type":"original"},{"month":"03","OA_place":"publisher","supervisor":[{"last_name":"Jonas","full_name":"Jonas, Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M","orcid":"0000-0001-5001-4804"}],"publisher":"Institute of Science and Technology Austria","citation":{"ista":"Chen J. 2024. Developmental transformation of nanodomain coupling between Ca2+ channels and release sensors at a central GABAergic synapse. Institute of Science and Technology Austria.","short":"J. Chen, Developmental Transformation of Nanodomain Coupling between Ca2+ Channels and Release Sensors at a Central GABAergic Synapse, Institute of Science and Technology Austria, 2024.","mla":"Chen, JingJing. <i>Developmental Transformation of Nanodomain Coupling between Ca2+ Channels and Release Sensors at a Central GABAergic Synapse</i>. Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/at:ista:15101\">10.15479/at:ista:15101</a>.","apa":"Chen, J. (2024). <i>Developmental transformation of nanodomain coupling between Ca2+ channels and release sensors at a central GABAergic synapse</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:15101\">https://doi.org/10.15479/at:ista:15101</a>","ieee":"J. Chen, “Developmental transformation of nanodomain coupling between Ca2+ channels and release sensors at a central GABAergic synapse,” Institute of Science and Technology Austria, 2024.","ama":"Chen J. Developmental transformation of nanodomain coupling between Ca2+ channels and release sensors at a central GABAergic synapse. 2024. doi:<a href=\"https://doi.org/10.15479/at:ista:15101\">10.15479/at:ista:15101</a>","chicago":"Chen, JingJing. “Developmental Transformation of Nanodomain Coupling between Ca2+ Channels and Release Sensors at a Central GABAergic Synapse.” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/at:ista:15101\">https://doi.org/10.15479/at:ista:15101</a>."},"has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"ec_funded":1,"acknowledged_ssus":[{"_id":"EM-Fac"}],"article_processing_charge":"No","page":"84","_id":"15101","corr_author":"1","status":"public","department":[{"_id":"GradSch"},{"_id":"PeJo"}],"file_date_updated":"2024-04-02T22:30:03Z","ddc":["570"],"degree_awarded":"PhD","related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"14843"}]},"oa":1,"date_updated":"2026-04-07T13:24:22Z","date_published":"2024-03-11T00:00:00Z","file":[{"checksum":"db4947474ffa271e66c254b6fe876a55","embargo_to":"open_access","file_id":"15104","file_name":"Thesis_Jingjing CHEN.docx","access_level":"closed","relation":"source_file","creator":"jchen","date_updated":"2024-04-02T22:30:03Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2024-03-11T14:10:58Z","file_size":11271363},{"checksum":"a5eeae8b5702cd540f5d03469bc33dde","file_name":"Thesis_Jingjing CHEN_merged.pdf","file_id":"15105","embargo":"2024-04-01","access_level":"open_access","relation":"main_file","creator":"jchen","date_updated":"2024-04-02T22:30:03Z","content_type":"application/pdf","date_created":"2024-03-11T14:11:06Z","file_size":16627311}],"abstract":[{"lang":"eng","text":"The coupling between presynaptic Ca2+ channels and release sensors is a key factor that\r\ndetermines speed and efficacy of synapse transmission. At some excitatory synapses,\r\nchannel–sensor coupling becomes tighter during development, and tightening is often\r\nassociated with a switch in the reliance on different Ca2+ channel subtypes. However, the\r\ncoupling topography at many synapses remains unknown, and it is unclear how it changes\r\nduring development. To address this question, we analyzed the coupling configuration at the\r\ncerebellar basket cell (BC) to Purkinje cell (PC) synapse at different developmental stages,\r\ncombining biophysical analysis, structural analysis, and modeling.\r\nQuantal analysis of BC–PC indicated that release probability decreased, while the\r\nnumber of functional sites increased during development. Although transmitter release\r\npersistently relied on P/Q-type Ca2+ channels in the time period postnatal day 7–23, effects\r\nof the Ca2+ chelator EGTA and BAPTA applied by intracellular pipette perfusion decreased\r\nduring development, indicative of tightening of source-sensor coupling. Furthermore,\r\npresynaptic action potentials became shorter during development, suggesting reduced\r\nefficacy of Ca2+ channel activation.\r\nStructural analysis by freeze-fracture replica labeling (FRL) and transmission electron\r\nmicroscopy (EM) indicated that presynaptic P/Q-type Ca2+ channels formed nanoclusters\r\nthroughout development, whereas docked vesicles were only clustered at later\r\ndevelopmental stages. The number of functional release sites correlated better with the AZ\r\nnumber early in development, but match better with the Ca2+ channel cluster number at later\r\nstages.\r\nModeling suggested a developmental transformation from a more random to a more\r\nclustered coupling nanotopography. Thus, presynaptic signaling developmentally approaches\r\na point-to-point configuration, optimizing speed, reliability, and energy efficiency of synaptic\r\ntransmission."}],"oa_version":"Published Version","day":"11","author":[{"id":"2C4E65C8-F248-11E8-B48F-1D18A9856A87","first_name":"JingJing","last_name":"Chen","full_name":"Chen, JingJing"}],"publication_status":"published","language":[{"iso":"eng"}],"title":"Developmental transformation of nanodomain coupling between Ca2+ channels and release sensors at a central GABAergic synapse","type":"dissertation","project":[{"call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse"},{"call_identifier":"FWF","_id":"25C5A090-B435-11E9-9278-68D0E5697425","grant_number":"Z00312","name":"Synaptic communication in neuronal microcircuits"},{"_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","name":"Mechanisms of GABA release in hippocampal circuits","grant_number":"P36232"},{"_id":"26B66A3E-B435-11E9-9278-68D0E5697425","name":"Development of nanodomain coupling between Ca2+ channels and release sensors at a central inhibitory synapse","grant_number":"25383"}],"alternative_title":["ISTA Thesis"],"date_created":"2024-03-11T10:09:54Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_identifier":{"issn":["2663-337X"]},"year":"2024","doi":"10.15479/at:ista:15101"},{"abstract":[{"lang":"eng","text":"The extracellular matrix (ECM) serves as a scaffold for cells and plays an essential role in regulating numerous cellular processes, including cell migration and proliferation. Due to limitations in specimen preparation for conventional room-temperature electron microscopy, we lack structural knowledge on how ECM components are secreted, remodeled, and interact with surrounding cells. We have developed a 3D-ECM platform compatible with sample thinning by cryo-focused ion beam milling, the lift-out extraction procedure, and cryo-electron tomography. Our workflow implements cell-derived matrices (CDMs) grown on EM grids, resulting in a versatile tool closely mimicking ECM environments. This allows us to visualize ECM for the first time in its hydrated, native context. Our data reveal an intricate network of extracellular fibers, their positioning relative to matrix-secreting cells, and previously unresolved structural entities. Our workflow and results add to the structural atlas of the ECM, providing novel insights into its secretion and assembly."}],"publication":"Journal of Cell Biology","file":[{"file_size":11907016,"success":1,"content_type":"application/pdf","date_created":"2024-03-25T12:52:04Z","date_updated":"2024-03-25T12:52:04Z","creator":"dernst","access_level":"open_access","relation":"main_file","file_name":"2024_JCB_Zens.pdf","file_id":"15188","checksum":"90d1984a93660735e506c2a304bc3f73"}],"date_published":"2024-03-20T00:00:00Z","external_id":{"pmid":["38506714"],"isi":["001264190100001"]},"author":[{"orcid":"0000-0002-9561-1239","last_name":"Zens","full_name":"Zens, Bettina","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","first_name":"Bettina"},{"orcid":"0000-0001-7149-769X","last_name":"Fäßler","full_name":"Fäßler, Florian","id":"404F5528-F248-11E8-B48F-1D18A9856A87","first_name":"Florian"},{"orcid":"0000-0001-7967-2085","last_name":"Hansen","full_name":"Hansen, Jesse","id":"1063c618-6f9b-11ec-9123-f912fccded63","first_name":"Jesse"},{"full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"orcid":"0000-0002-3616-8580","full_name":"Datler, Julia","last_name":"Datler","first_name":"Julia","id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-3904-947X","full_name":"Hodirnau, Victor-Valentin","last_name":"Hodirnau","first_name":"Victor-Valentin","id":"3661B498-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-9438-4783","full_name":"Zheden, Vanessa","last_name":"Zheden","first_name":"Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-7698-3061","first_name":"Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","full_name":"Alanko, Jonna H","last_name":"Alanko"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K"},{"orcid":"0000-0003-4790-8078","last_name":"Schur","full_name":"Schur, Florian KM","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM"}],"day":"20","pmid":1,"oa_version":"Published Version","issue":"6","type":"journal_article","title":"Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix","project":[{"_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","grant_number":"P33367","name":"Structure and isoform diversity of the Arp2/3 complex"},{"_id":"7bd318a1-9f16-11ee-852c-cc9217763180","grant_number":"E435","name":"In Situ Actin Structures via Hybrid Cryo-electron Microscopy"},{"grant_number":"724373","name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"_id":"059B463C-7A3F-11EA-A408-12923DDC885E","name":"NÖ-Fonds Preis für die Jungforscherin des Jahres am IST Austria"},{"_id":"2615199A-B435-11E9-9278-68D0E5697425","name":"Spatiotemporal regulation of chemokine-induced signalling in leukocyte chemotaxis","grant_number":"21317"},{"_id":"62909c6f-2b32-11ec-9570-e1476aab5308","grant_number":"CZI01","name":"CryoMinflux-guided in-situ visual proteomics and structure determination"}],"language":[{"iso":"eng"}],"isi":1,"publication_status":"published","article_type":"original","acknowledgement":"Open Access funding provided by IST Austria. We thank Armel Nicolas and his team at the ISTA proteomics facility, Alois Schloegl, Stefano Elefante, and colleagues at the ISTA Scientific Computing facility, Tommaso Constanzo and Ludek Lovicar at the Electron Microsocpy Facility (EMF), and Thomas Menner at the Miba Machine shop for their support. We also thank Wanda Kukulski (University of Bern) as well as Darío Porley, Andreas Thader, and other members of the Schur group for helpful discussions. Matt Swulius and Jessica Heebner provided great support in using Dragonfly. We thank Dorotea Fracciolla (Art & Science) for support in figure illustration.\r\n\r\nThis research was supported by the Scientific Service Units of ISTA through resources provided by Scientific Computing, the Lab Support Facility, and the Electron Microscopy Facility. We acknowledge funding support from the following sources: Austrian Science Fund (FWF) grant P33367 (to F.K.M. Schur), the Federation of European Biochemical Societies (to F.K.M. Schur), Niederösterreich (NÖ) Fonds (to B. Zens), FWF grant E435 (to J.M. Hansen), European Research Council under the European Union’s Horizon 2020 research (grant agreement No. 724373) (to M. Sixt), and Jenny and Antti Wihuri Foundation (to J. Alanko). This publication has been made possible in part by CZI grant DAF2021-234754 and grant DOI https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (to F.K.M. Schur).","doi":"10.1083/jcb.202309125","year":"2024","article_number":"e202309125","publication_identifier":{"eissn":["1540-8140"],"issn":["0021-9525"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-03-21T06:45:51Z","intvolume":"       223","publisher":"Rockefeller University Press","month":"03","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"Yes (via OA deal)","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"M-Shop"}],"ec_funded":1,"quality_controlled":"1","volume":223,"citation":{"mla":"Zens, Bettina, et al. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” <i>Journal of Cell Biology</i>, vol. 223, no. 6, e202309125, Rockefeller University Press, 2024, doi:<a href=\"https://doi.org/10.1083/jcb.202309125\">10.1083/jcb.202309125</a>.","apa":"Zens, B., Fäßler, F., Hansen, J., Hauschild, R., Datler, J., Hodirnau, V.-V., … Schur, F. K. (2024). Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202309125\">https://doi.org/10.1083/jcb.202309125</a>","short":"B. Zens, F. Fäßler, J. Hansen, R. Hauschild, J. Datler, V.-V. Hodirnau, V. Zheden, J.H. Alanko, M.K. Sixt, F.K. Schur, Journal of Cell Biology 223 (2024).","ista":"Zens B, Fäßler F, Hansen J, Hauschild R, Datler J, Hodirnau V-V, Zheden V, Alanko JH, Sixt MK, Schur FK. 2024. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. Journal of Cell Biology. 223(6), e202309125.","chicago":"Zens, Bettina, Florian Fäßler, Jesse Hansen, Robert Hauschild, Julia Datler, Victor-Valentin Hodirnau, Vanessa Zheden, Jonna H Alanko, Michael K Sixt, and Florian KM Schur. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2024. <a href=\"https://doi.org/10.1083/jcb.202309125\">https://doi.org/10.1083/jcb.202309125</a>.","ama":"Zens B, Fäßler F, Hansen J, et al. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. <i>Journal of Cell Biology</i>. 2024;223(6). doi:<a href=\"https://doi.org/10.1083/jcb.202309125\">10.1083/jcb.202309125</a>","ieee":"B. Zens <i>et al.</i>, “Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix,” <i>Journal of Cell Biology</i>, vol. 223, no. 6. Rockefeller University Press, 2024."},"has_accepted_license":"1","scopus_import":"1","department":[{"_id":"FlSc"},{"_id":"MiSi"},{"_id":"Bio"},{"_id":"EM-Fac"}],"file_date_updated":"2024-03-25T12:52:04Z","ddc":["570"],"status":"public","corr_author":"1","_id":"15146","date_updated":"2025-09-04T13:17:16Z","oa":1},{"acknowledgement":"This work was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Electron Microscopy Facility (EMF), the Lab Support Facility (LSF), and the Nanofabrication Facility (NNF). This work was financially supported by ISTA and the Werner Siemens Foundation. The USTEM Service Unit of the Technical University of Vienna is acknowledged for EBSD sample preparation and analysis. R.L.B. acknowledges the National Science Foundation for funding the mass spectrometry analysis under award DMR 1904719. J.L. is a Serra Húnter Fellow and is grateful to the ICREA Academia program and projects MICINN/FEDER PID2021-124572OB-C31 and GC 2021 SGR 01061.","doi":"10.1002/aenm.202400408","year":"2024","article_number":"2400408","publication_identifier":{"eissn":["1614-6840"],"issn":["1614-6832"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-03-25T08:57:40Z","title":"A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se","type":"journal_article","project":[{"_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A","name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery"}],"language":[{"iso":"eng"}],"isi":1,"publication_status":"published","article_type":"original","author":[{"orcid":"0000-0003-1537-7436","first_name":"Tobias","id":"8BD9DE16-AB3C-11E9-9C8C-2A03E6697425","full_name":"Kleinhanns, Tobias","last_name":"Kleinhanns"},{"first_name":"Francesco","id":"38b830db-ea88-11ee-bf9b-929beaf79054","full_name":"Milillo, Francesco","last_name":"Milillo"},{"orcid":"0000-0003-4566-5877","last_name":"Calcabrini","full_name":"Calcabrini, Mariano","id":"45D7531A-F248-11E8-B48F-1D18A9856A87","first_name":"Mariano"},{"id":"bd3fceba-dc74-11ea-a0a7-c17f71817366","first_name":"Christine","last_name":"Fiedler","full_name":"Fiedler, Christine"},{"first_name":"Sharona","id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc","full_name":"Horta, Sharona","last_name":"Horta"},{"last_name":"Balazs","full_name":"Balazs, Daniel","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","first_name":"Daniel","orcid":"0000-0001-7597-043X"},{"first_name":"Marissa J.","last_name":"Strumolo","full_name":"Strumolo, Marissa J."},{"first_name":"Roger","last_name":"Hasler","full_name":"Hasler, Roger"},{"full_name":"Llorca, Jordi","last_name":"Llorca","first_name":"Jordi"},{"full_name":"Tkadletz, Michael","last_name":"Tkadletz","first_name":"Michael"},{"first_name":"Richard L.","full_name":"Brutchey, Richard L.","last_name":"Brutchey"},{"id":"43C61214-F248-11E8-B48F-1D18A9856A87","first_name":"Maria","last_name":"Ibáñez","full_name":"Ibáñez, Maria","orcid":"0000-0001-5013-2843"}],"day":"12","oa_version":"Published Version","issue":"22","abstract":[{"text":"Thermoelectric materials convert heat into electricity, with a broad range of applications near room temperature (RT). However, the library of RT high-performance materials is limited. Traditional high-temperature synthetic methods constrain the range of materials achievable, hindering the ability to surpass crystal structure limitations and engineer defects. Here, a solution-based synthetic approach is introduced, enabling RT synthesis of powders and exploration of densification at lower temperatures to influence the material's microstructure. The approach is exemplified by Ag2Se, an n-type alternative to bismuth telluride. It is demonstrated that the concentration of Ag interstitials, grain boundaries, and dislocations are directly correlated to the sintering temperature, and achieve a figure of merit of 1.1 from RT to 100 °C after optimization. Moreover, insights into and resolve Ag2Se's challenges are provided, including stoichiometry issues leading to irreproducible performances. This work highlights the potential of RT solution synthesis in expanding the repertoire of high-performance thermoelectric materials for practical applications.","lang":"eng"}],"publication":"Advanced Energy Materials","file":[{"file_size":8824301,"date_created":"2024-07-22T12:07:56Z","content_type":"application/pdf","success":1,"date_updated":"2024-07-22T12:07:56Z","creator":"dernst","access_level":"open_access","relation":"main_file","file_id":"17314","file_name":"2024_AdvancedEnergyMaterials_Kleinhanns.pdf","checksum":"86b26430e00d5f43ea19e9b610692ab7"}],"external_id":{"isi":["001184300200001"]},"date_published":"2024-06-12T00:00:00Z","date_updated":"2026-06-22T06:14:35Z","oa":1,"related_material":{"record":[{"id":"22017","status":"for_moderation","relation":"dissertation_contains"}]},"scopus_import":"1","department":[{"_id":"MaIb"},{"_id":"LifeSc"}],"file_date_updated":"2024-07-22T12:07:56Z","ddc":["530"],"status":"public","_id":"15182","corr_author":"1","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)"},"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"NanoFab"}],"article_processing_charge":"Yes (via OA deal)","volume":14,"quality_controlled":"1","citation":{"ama":"Kleinhanns T, Milillo F, Calcabrini M, et al. A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se. <i>Advanced Energy Materials</i>. 2024;14(22). doi:<a href=\"https://doi.org/10.1002/aenm.202400408\">10.1002/aenm.202400408</a>","chicago":"Kleinhanns, Tobias, Francesco Milillo, Mariano Calcabrini, Christine Fiedler, Sharona Horta, Daniel Balazs, Marissa J. Strumolo, et al. “A Route to High Thermoelectric Performance: Solution‐based Control of Microstructure and Composition in Ag2Se.” <i>Advanced Energy Materials</i>. Wiley, 2024. <a href=\"https://doi.org/10.1002/aenm.202400408\">https://doi.org/10.1002/aenm.202400408</a>.","ieee":"T. Kleinhanns <i>et al.</i>, “A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se,” <i>Advanced Energy Materials</i>, vol. 14, no. 22. Wiley, 2024.","apa":"Kleinhanns, T., Milillo, F., Calcabrini, M., Fiedler, C., Horta, S., Balazs, D., … Ibáñez, M. (2024). A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se. <i>Advanced Energy Materials</i>. Wiley. <a href=\"https://doi.org/10.1002/aenm.202400408\">https://doi.org/10.1002/aenm.202400408</a>","mla":"Kleinhanns, Tobias, et al. “A Route to High Thermoelectric Performance: Solution‐based Control of Microstructure and Composition in Ag2Se.” <i>Advanced Energy Materials</i>, vol. 14, no. 22, 2400408, Wiley, 2024, doi:<a href=\"https://doi.org/10.1002/aenm.202400408\">10.1002/aenm.202400408</a>.","short":"T. Kleinhanns, F. Milillo, M. Calcabrini, C. Fiedler, S. Horta, D. Balazs, M.J. Strumolo, R. Hasler, J. Llorca, M. Tkadletz, R.L. Brutchey, M. Ibáñez, Advanced Energy Materials 14 (2024).","ista":"Kleinhanns T, Milillo F, Calcabrini M, Fiedler C, Horta S, Balazs D, Strumolo MJ, Hasler R, Llorca J, Tkadletz M, Brutchey RL, Ibáñez M. 2024. A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se. Advanced Energy Materials. 14(22), 2400408."},"has_accepted_license":"1","intvolume":"        14","publisher":"Wiley","month":"06"},{"publication_identifier":{"eissn":["1545-9985"],"issn":["1545-9993"]},"year":"2024","doi":"10.1038/s41594-024-01255-0","acknowledgement":"Supercomplexes of the respiratory chain are established constituents of the oxidative phosphorylation system, but their role in mammalian metabolism has been hotly debated. Although recent studies have shown that different tissues/organs are equipped with specific sets of supercomplexes, depending on their metabolic needs, the notion that supercomplexes have a role in the regulation of metabolism has been challenged. However, irrespective of the mechanistic conclusions, the composition of various high molecular weight supercomplexes remains uncertain. Here, using cryogenic electron microscopy, we demonstrate that mammalian (mouse) tissues contain three defined types of ‘respirasome’, supercomplexes made of CI, CIII2 and CIV. The stoichiometry and position of CIV differs in the three respirasomes, of which only one contains the supercomplex-associated factor SCAF1, whose involvement in respirasome formation has long been contended. Our structures confirm that the ‘canonical’ respirasome (the C-respirasome, CICIII2CIV) does not contain SCAF1, which is instead associated to a different respirasome (the CS-respirasome), containing a second copy of CIV. We also identify an alternative respirasome (A-respirasome), with CIV bound to the ‘back’ of CI, instead of the ‘toe’. This structural characterization of mouse mitochondrial supercomplexes allows us to hypothesize a mechanistic basis for their specific role in different metabolic conditions.","date_created":"2024-04-14T22:01:03Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","isi":1,"language":[{"iso":"eng"}],"type":"journal_article","title":"SCAF1 drives the compositional diversity of mammalian respirasomes","project":[{"call_identifier":"H2020","grant_number":"101020697","name":"Structure and mechanism of respiratory chain molecular machines","_id":"627abdeb-2b32-11ec-9570-ec31a97243d3"}],"article_type":"original","publication_status":"published","day":"01","author":[{"orcid":"0000-0001-5618-3449","last_name":"Vercellino","full_name":"Vercellino, Irene","id":"3ED6AF16-F248-11E8-B48F-1D18A9856A87","first_name":"Irene"},{"full_name":"Sazanov, Leonid A","last_name":"Sazanov","first_name":"Leonid A","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0977-7989"}],"oa_version":"Submitted Version","pmid":1,"abstract":[{"lang":"eng","text":"Supercomplexes of the respiratory chain are established constituents of the oxidative phosphorylation system, but their role in mammalian metabolism has been hotly debated. Although recent studies have shown that different tissues/organs are equipped with specific sets of supercomplexes, depending on their metabolic needs, the notion that supercomplexes have a role in the regulation of metabolism has been challenged. However, irrespective of the mechanistic conclusions, the composition of various high molecular weight supercomplexes remains uncertain. Here, using cryogenic electron microscopy, we demonstrate that mammalian (mouse) tissues contain three defined types of ‘respirasome’, supercomplexes made of CI, CIII2 and CIV. The stoichiometry and position of CIV differs in the three respirasomes, of which only one contains the supercomplex-associated factor SCAF1, whose involvement in respirasome formation has long been contended. Our structures confirm that the ‘canonical’ respirasome (the C-respirasome, CICIII2CIV) does not contain SCAF1, which is instead associated to a different respirasome (the CS-respirasome), containing a second copy of CIV. We also identify an alternative respirasome (A-respirasome), with CIV bound to the ‘back’ of CI, instead of the ‘toe’. This structural characterization of mouse mitochondrial supercomplexes allows us to hypothesize a mechanistic basis for their specific role in different metabolic conditions."}],"external_id":{"pmid":["38575788"],"isi":["001196897300001"]},"date_published":"2024-07-01T00:00:00Z","file":[{"file_size":24424729,"content_type":"application/pdf","date_created":"2024-05-14T11:57:56Z","date_updated":"2025-01-01T23:30:03Z","creator":"lsazanov","embargo":"2025-01-01","relation":"main_file","access_level":"open_access","file_id":"15392","file_name":"megacomplex_submit_NSMB_withFigures.pdf","checksum":"21f05d188762acd7f49a97f3d09c8d9f"}],"publication":"Nature Structural and Molecular Biology","date_updated":"2025-11-24T08:35:04Z","oa":1,"related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1038/s41594-025-01721-3"}]},"department":[{"_id":"LeSa"}],"file_date_updated":"2025-01-01T23:30:03Z","ddc":["572"],"scopus_import":"1","corr_author":"1","_id":"15323","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"ec_funded":1,"article_processing_charge":"No","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"PreCl"},{"_id":"ScienComp"}],"page":"1061-1071","citation":{"ama":"Vercellino I, Sazanov LA. SCAF1 drives the compositional diversity of mammalian respirasomes. <i>Nature Structural and Molecular Biology</i>. 2024;31:1061-1071. doi:<a href=\"https://doi.org/10.1038/s41594-024-01255-0\">10.1038/s41594-024-01255-0</a>","chicago":"Vercellino, Irene, and Leonid A Sazanov. “SCAF1 Drives the Compositional Diversity of Mammalian Respirasomes.” <i>Nature Structural and Molecular Biology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41594-024-01255-0\">https://doi.org/10.1038/s41594-024-01255-0</a>.","ieee":"I. Vercellino and L. A. Sazanov, “SCAF1 drives the compositional diversity of mammalian respirasomes,” <i>Nature Structural and Molecular Biology</i>, vol. 31. Springer Nature, pp. 1061–1071, 2024.","mla":"Vercellino, Irene, and Leonid A. Sazanov. “SCAF1 Drives the Compositional Diversity of Mammalian Respirasomes.” <i>Nature Structural and Molecular Biology</i>, vol. 31, Springer Nature, 2024, pp. 1061–71, doi:<a href=\"https://doi.org/10.1038/s41594-024-01255-0\">10.1038/s41594-024-01255-0</a>.","apa":"Vercellino, I., &#38; Sazanov, L. A. (2024). SCAF1 drives the compositional diversity of mammalian respirasomes. <i>Nature Structural and Molecular Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41594-024-01255-0\">https://doi.org/10.1038/s41594-024-01255-0</a>","short":"I. Vercellino, L.A. Sazanov, Nature Structural and Molecular Biology 31 (2024) 1061–1071.","ista":"Vercellino I, Sazanov LA. 2024. SCAF1 drives the compositional diversity of mammalian respirasomes. Nature Structural and Molecular Biology. 31, 1061–1071."},"has_accepted_license":"1","volume":31,"quality_controlled":"1","publisher":"Springer Nature","intvolume":"        31","month":"07"},{"date_created":"2024-04-19T09:54:59Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"article_number":"jcs.261720","year":"2024","doi":"10.1242/jcs.261720","acknowledgement":"Nataliia Gnyliukh was partially funded by the European Union’s Horizon 2020 research and\r\ninnovation program (2018-2020) under the Marie Sklodowska-Curie Grant (agreement no.\r\n665385). Taif University Researchers Supporting Project: TURSP-HC2022/02. and Austrian\r\nScience Fund (FWF): I 6123-B.We thank Prof. Eileen Lafer and Liping Wang for their suggestions regarding the optimisation of protein expression and purification. We thank Prof. Sebastian Y. Bednarek for the useful comments and constructive criticism of the project. We thank Maciek Adamowski for providing genetic material. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Electron microscopy (EMF), Lab Support Facility (LSF) (particularly Dorota Jaworska) and the Bioimaging Facility (BIF).","article_type":"original","publication_status":"published","language":[{"iso":"eng"}],"isi":1,"project":[{"grant_number":"665385","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"}],"title":"Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana","type":"journal_article","issue":"8","pmid":1,"oa_version":"Published Version","day":"01","author":[{"full_name":"Gnyliukh, Nataliia","last_name":"Gnyliukh","first_name":"Nataliia","id":"390C1120-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2198-0509"},{"orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","last_name":"Johnson","full_name":"Johnson, Alexander J"},{"full_name":"Nagel, MK","last_name":"Nagel","first_name":"MK"},{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","first_name":"Aline","last_name":"Monzer","full_name":"Monzer, Aline"},{"id":"db566d23-f6e0-11ea-865d-e6f270e968e7","first_name":"David","last_name":"Babic","full_name":"Babic, David"},{"first_name":"Annamaria","id":"36062FEC-F248-11E8-B48F-1D18A9856A87","full_name":"Hlavata, Annamaria","last_name":"Hlavata"},{"full_name":"Alotaibi, SS","last_name":"Alotaibi","first_name":"SS"},{"first_name":"E","last_name":"Isono","full_name":"Isono, E"},{"orcid":"0000-0001-7309-9724","id":"462D4284-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","last_name":"Loose","full_name":"Loose, Martin"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"external_id":{"isi":["001266917100005"],"pmid":["38506228"]},"file":[{"file_id":"18792","file_name":"2024_JourCellScience_Gnyliukh.pdf","relation":"main_file","access_level":"open_access","checksum":"6dc023f0cc7052ad3cf0a42589d2e30f","content_type":"application/pdf","date_created":"2025-01-09T08:41:16Z","success":1,"file_size":25845948,"creator":"dernst","date_updated":"2025-01-09T08:41:16Z"}],"date_published":"2024-04-01T00:00:00Z","publication":"Journal of Cell Science","abstract":[{"text":"Clathrin-mediated endocytosis (CME) is vital for the regulation of plant growth and development by controlling plasma membrane protein composition and cargo uptake. CME relies on the precise recruitment of regulators for vesicle maturation and release. Homologues of components of mammalian vesicle scission are strong candidates to be part of the scission machinery in plants, but the precise roles of these proteins in this process are not fully understood. Here, we characterised the roles of Plant Dynamin-Related Proteins 2 (DRP2s) and SH3-domain containing protein 2 (SH3P2), the plant homologue to Dynamins’ recruiters, like Endophilin and Amphiphysin, in the CME by combining high-resolution imaging of endocytic events in vivo and characterisation of the purified proteins in vitro. Although DRP2s and SH3P2 arrive similarly late during CME and physically interact, genetic analysis of the sh3p123 triple-mutant and complementation assays with non-SH3P2-interacting DRP2 variants suggests that SH3P2 does not directly recruit DRP2s to the site of endocytosis. These observations imply that despite the presence of many well-conserved endocytic components, plants have acquired a distinct mechanism for CME.","lang":"eng"}],"related_material":{"record":[{"status":"public","relation":"earlier_version","id":"14591"}]},"oa":1,"date_updated":"2025-09-04T13:49:45Z","_id":"15330","corr_author":"1","status":"public","ddc":["570"],"department":[{"_id":"MaLo"},{"_id":"JiFr"},{"_id":"CaBe"}],"file_date_updated":"2025-01-09T08:41:16Z","scopus_import":"1","has_accepted_license":"1","citation":{"ama":"Gnyliukh N, Johnson AJ, Nagel M, et al. Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. <i>Journal of Cell Science</i>. 2024;137(8). doi:<a href=\"https://doi.org/10.1242/jcs.261720\">10.1242/jcs.261720</a>","chicago":"Gnyliukh, Nataliia, Alexander J Johnson, MK Nagel, Aline Monzer, David Babic, Annamaria Hlavata, SS Alotaibi, E Isono, Martin Loose, and Jiří Friml. “Role of Dynamin-Related Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Arabidopsis Thaliana.” <i>Journal of Cell Science</i>. The Company of Biologists, 2024. <a href=\"https://doi.org/10.1242/jcs.261720\">https://doi.org/10.1242/jcs.261720</a>.","ieee":"N. Gnyliukh <i>et al.</i>, “Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana,” <i>Journal of Cell Science</i>, vol. 137, no. 8. The Company of Biologists, 2024.","mla":"Gnyliukh, Nataliia, et al. “Role of Dynamin-Related Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Arabidopsis Thaliana.” <i>Journal of Cell Science</i>, vol. 137, no. 8, jcs. 261720, The Company of Biologists, 2024, doi:<a href=\"https://doi.org/10.1242/jcs.261720\">10.1242/jcs.261720</a>.","apa":"Gnyliukh, N., Johnson, A. J., Nagel, M., Monzer, A., Babic, D., Hlavata, A., … Friml, J. (2024). Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.261720\">https://doi.org/10.1242/jcs.261720</a>","ista":"Gnyliukh N, Johnson AJ, Nagel M, Monzer A, Babic D, Hlavata A, Alotaibi S, Isono E, Loose M, Friml J. 2024. Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. Journal of Cell Science. 137(8), jcs. 261720.","short":"N. Gnyliukh, A.J. Johnson, M. Nagel, A. Monzer, D. Babic, A. Hlavata, S. Alotaibi, E. Isono, M. Loose, J. Friml, Journal of Cell Science 137 (2024)."},"OA_type":"hybrid","quality_controlled":"1","volume":137,"article_processing_charge":"Yes (via OA deal)","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"Bio"}],"ec_funded":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"OA_place":"publisher","month":"04","publisher":"The Company of Biologists","intvolume":"       137"}]