[{"publisher":"Springer Nature","abstract":[{"lang":"eng","text":"Reproducible pattern and form generation during embryogenesis is poorly understood. Intestinal organoid morphogenesis involves a number of mechanochemical regulators such as cell-type-specific cytoskeletal forces and osmotically driven lumen volume changes. It is unclear how these forces are coordinated in time and space to ensure robust morphogenesis. Here we show how mechanosensitive feedback on cytoskeletal tension gives rise to morphological bistability in a minimal model of organoid morphogenesis. In the model, lumen volume changes can impact the epithelial shape via both direct mechanical and indirect mechanosensitive mechanisms. We find that both bulged and budded crypt states are possible and dependent on the history of volume changes. We test key modelling assumptions via biophysical and pharmacological experiments to demonstrate how bistability can explain experimental observations, such as the importance of the timing of lumen shrinkage and robustness of the final morphogenetic state to mechanical perturbations. This suggests that bistability arising from feedback between cellular tensions and fluid pressure could be a general mechanism that coordinates multicellular shape changes in developing systems."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"corr_author":"1","author":[{"id":"31D2C804-F248-11E8-B48F-1D18A9856A87","first_name":"Shi-lei","full_name":"Xue, Shi-lei","last_name":"Xue"},{"full_name":"Yang, Qiutan","first_name":"Qiutan","last_name":"Yang"},{"last_name":"Liberali","first_name":"Prisca","full_name":"Liberali, Prisca"},{"full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","last_name":"Hannezo"}],"oa":1,"file_date_updated":"2025-08-05T12:12:03Z","intvolume":"        21","quality_controlled":"1","status":"public","OA_type":"hybrid","ddc":["530"],"PlanS_conform":"1","pmid":1,"scopus_import":"1","publication":"Nature Physics","volume":21,"year":"2025","date_updated":"2025-09-30T10:47:36Z","file":[{"creator":"dernst","date_updated":"2025-08-05T12:12:03Z","access_level":"open_access","date_created":"2025-08-05T12:12:03Z","file_name":"2025_NaturePhysics_Xue.pdf","success":1,"checksum":"fb5e59be145b95f9851d3d7c9dbb85e6","content_type":"application/pdf","relation":"main_file","file_id":"20129","file_size":16302436}],"acknowledgement":"We thank all members of the Hannezo and Liberali groups for fruitful discussions, as well as C. Schwayer, G. Quintas, L. Capolupo, D. Bruckner and D. Pinheiro for reading the manuscript. We also thank Y. Wu and X. Wu from the Yang group for performing experiments in the last rounds of revision and the So group at the National Institute of Biological Sciences, Beijing, for helping with the light-sheet time-lapse experiments. This work received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme via grant agreement no. 758617 (to P.L.), Swiss National Foundation (SNF) (no. POOP3_157531 to P.L.), the ERC under the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 851288 (to E.H.) and the Austrian Science Fund (FWF) (no. P 31639 to E.H.). This work was supported by the National Natural Science Foundation of China via grant no.3247060387 (to Q.Y.) and the Strategic Priority Research Program of the Chinese Academy of Sciences (no. XDB0820000 to Q.Y.) . Open access funding provided by Institute of Science and Technology (IST Austria).","date_created":"2025-03-09T23:01:28Z","ec_funded":1,"department":[{"_id":"EdHa"}],"date_published":"2025-02-28T00:00:00Z","OA_place":"publisher","month":"02","isi":1,"arxiv":1,"article_type":"original","has_accepted_license":"1","project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"},{"grant_number":"P31639","name":"Active mechano-chemical description of the cell cytoskeleton","call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425"}],"type":"journal_article","article_processing_charge":"Yes (via OA deal)","doi":"10.1038/s41567-025-02792-1","fulldoi":"https://doi.org/10.1038/s41567-025-02792-1","language":[{"iso":"eng"}],"oa_version":"Published Version","_id":"19373","day":"28","citation":{"short":"S. Xue, Q. Yang, P. Liberali, E.B. Hannezo, Nature Physics 21 (2025).","ama":"Xue S, Yang Q, Liberali P, Hannezo EB. Mechanochemical bistability of intestinal organoids enables robust morphogenesis. <i>Nature Physics</i>. 2025;21. doi:<a href=\"https://doi.org/10.1038/s41567-025-02792-1\">10.1038/s41567-025-02792-1</a>","mla":"Xue, Shi-lei, et al. “Mechanochemical Bistability of Intestinal Organoids Enables Robust Morphogenesis.” <i>Nature Physics</i>, vol. 21, 078104, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41567-025-02792-1\">10.1038/s41567-025-02792-1</a>.","ieee":"S. Xue, Q. Yang, P. Liberali, and E. B. Hannezo, “Mechanochemical bistability of intestinal organoids enables robust morphogenesis,” <i>Nature Physics</i>, vol. 21. Springer Nature, 2025.","apa":"Xue, S., Yang, Q., Liberali, P., &#38; Hannezo, E. B. (2025). Mechanochemical bistability of intestinal organoids enables robust morphogenesis. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-02792-1\">https://doi.org/10.1038/s41567-025-02792-1</a>","chicago":"Xue, Shi-lei, Qiutan Yang, Prisca Liberali, and Edouard B Hannezo. “Mechanochemical Bistability of Intestinal Organoids Enables Robust Morphogenesis.” <i>Nature Physics</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41567-025-02792-1\">https://doi.org/10.1038/s41567-025-02792-1</a>.","ista":"Xue S, Yang Q, Liberali P, Hannezo EB. 2025. Mechanochemical bistability of intestinal organoids enables robust morphogenesis. Nature Physics. 21, 078104."},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"078104","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"publication_status":"published","external_id":{"arxiv":["2403.19900"],"isi":["001434072800001"],"pmid":["40248571"]},"title":"Mechanochemical bistability of intestinal organoids enables robust morphogenesis"},{"citation":{"ista":"Gu AA, Ucar MC, Tran P, Prindle A, Kamat NP, Steinkühler J. 2025. Remodeling of lipid-foam prototissues by network-wide tension fluctuations induced by active particles. Nature Communications. 16, 2026.","chicago":"Gu, Andre A., Mehmet C Ucar, Peter Tran, Arthur Prindle, Neha P. Kamat, and Jan Steinkühler. “Remodeling of Lipid-Foam Prototissues by Network-Wide Tension Fluctuations Induced by Active Particles.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-57178-x\">https://doi.org/10.1038/s41467-025-57178-x</a>.","mla":"Gu, Andre A., et al. “Remodeling of Lipid-Foam Prototissues by Network-Wide Tension Fluctuations Induced by Active Particles.” <i>Nature Communications</i>, vol. 16, 2026, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-57178-x\">10.1038/s41467-025-57178-x</a>.","ieee":"A. A. Gu, M. C. Ucar, P. Tran, A. Prindle, N. P. Kamat, and J. Steinkühler, “Remodeling of lipid-foam prototissues by network-wide tension fluctuations induced by active particles,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","short":"A.A. Gu, M.C. Ucar, P. Tran, A. Prindle, N.P. Kamat, J. Steinkühler, Nature Communications 16 (2025).","ama":"Gu AA, Ucar MC, Tran P, Prindle A, Kamat NP, Steinkühler J. Remodeling of lipid-foam prototissues by network-wide tension fluctuations induced by active particles. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-57178-x\">10.1038/s41467-025-57178-x</a>","apa":"Gu, A. A., Ucar, M. C., Tran, P., Prindle, A., Kamat, N. P., &#38; Steinkühler, J. (2025). Remodeling of lipid-foam prototissues by network-wide tension fluctuations induced by active particles. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-57178-x\">https://doi.org/10.1038/s41467-025-57178-x</a>"},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"2026","publication_status":"published","publication_identifier":{"eissn":["2041-1723"]},"external_id":{"isi":["001435269000002"],"pmid":["40016255"]},"title":"Remodeling of lipid-foam prototissues by network-wide tension fluctuations induced by active particles","isi":1,"article_type":"original","has_accepted_license":"1","type":"journal_article","article_processing_charge":"Yes (via OA deal)","doi":"10.1038/s41467-025-57178-x","fulldoi":"https://doi.org/10.1038/s41467-025-57178-x","oa_version":"Published Version","language":[{"iso":"eng"}],"day":"27","_id":"19402","date_updated":"2025-09-30T10:59:30Z","file":[{"checksum":"3bbae9b470c639005815342a39e96918","success":1,"date_created":"2025-03-17T09:43:27Z","file_name":"2025_NatureComm_Gu.pdf","creator":"dernst","access_level":"open_access","date_updated":"2025-03-17T09:43:27Z","file_size":2260791,"file_id":"19411","relation":"main_file","content_type":"application/pdf"}],"acknowledgement":"This research was supported in part by the National Science Foundation under Grant No. 1844336 (J.S.), 2239567 (A.P), and MRSEC DMR-2308691 (A.G., N.P.K.) and the National Institutes of Health under Grant No. 1R35GM147170-01 (A.P). J.S. thanks Reinhard Lipowsky for discussions on stability of foams.\r\nOpen Access funding enabled and organized by Projekt DEAL.","date_created":"2025-03-16T23:01:23Z","department":[{"_id":"EdHa"}],"date_published":"2025-02-27T00:00:00Z","OA_place":"publisher","month":"02","publisher":"Springer Nature","abstract":[{"lang":"eng","text":"Recent advances in the field of bottom-up synthetic biology have led to the development of synthetic cells that mimic some features of real cells, such as division, protein synthesis, or DNA replication. Larger assemblies of synthetic cells may be used to form prototissues. However, existing prototissues are limited by their relatively small lateral dimensions or their lack of remodeling ability. Here, we introduce a lipid-based tissue mimetic that can be easily prepared and functionalized, consisting of a millimeter-sized “lipid-foam” with individual micrometer-sized compartments bound by lipid bilayers. We characterize the structural and mechanical properties of the lipid-foam tissue mimetic, and we demonstrate self-healing capabilities enabled by the fluidity of the lipid bilayers. Upon inclusion of bacteria in the tissue compartments, we observe that the tissue mimetic exhibits network-wide tension fluctuations driven by membrane tension generation by the swimming bacteria. Active tension fluctuations facilitate the fluidization and reorganization of the prototissue, providing a versatile platform for understanding and mimicking biological tissues."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"author":[{"last_name":"Gu","first_name":"Andre A.","full_name":"Gu, Andre A."},{"first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425","orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C","last_name":"Ucar"},{"first_name":"Peter","full_name":"Tran, Peter","last_name":"Tran"},{"full_name":"Prindle, Arthur","first_name":"Arthur","last_name":"Prindle"},{"last_name":"Kamat","first_name":"Neha P.","full_name":"Kamat, Neha P."},{"last_name":"Steinkühler","full_name":"Steinkühler, Jan","first_name":"Jan"}],"oa":1,"file_date_updated":"2025-03-17T09:43:27Z","intvolume":"        16","OA_type":"gold","status":"public","quality_controlled":"1","ddc":["570"],"DOAJ_listed":"1","scopus_import":"1","pmid":1,"publication":"Nature Communications","year":"2025","volume":16},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance","publication_status":"published","publication_identifier":{"issn":["0022-202X"],"eissn":["1523-1747"]},"external_id":{"pmid":["40010488"],"isi":["001604396400001"]},"citation":{"apa":"Andersen, M. S., Ulyanchenko, S., Schweiger, P. J., Hannezo, E. B., Simons, B. D., &#38; Jensen, K. B. (2025). Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance. <i>Journal of Investigative Dermatology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">https://doi.org/10.1016/j.jid.2025.01.034</a>","short":"M.S. Andersen, S. Ulyanchenko, P.J. Schweiger, E.B. Hannezo, B.D. Simons, K.B. Jensen, Journal of Investigative Dermatology 145 (2025) 2191–2202.e5.","ama":"Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB. Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance. <i>Journal of Investigative Dermatology</i>. 2025;145(9):2191-2202.e5. doi:<a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">10.1016/j.jid.2025.01.034</a>","ieee":"M. S. Andersen, S. Ulyanchenko, P. J. Schweiger, E. B. Hannezo, B. D. Simons, and K. B. Jensen, “Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance,” <i>Journal of Investigative Dermatology</i>, vol. 145, no. 9. Elsevier, p. 2191–2202.e5, 2025.","mla":"Andersen, Marianne S., et al. “Spatiotemporal Switches in Progenitor Cell Fate Govern Upper Hair Follicle Growth and Maintenance.” <i>Journal of Investigative Dermatology</i>, vol. 145, no. 9, Elsevier, 2025, p. 2191–2202.e5, doi:<a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">10.1016/j.jid.2025.01.034</a>.","chicago":"Andersen, Marianne S., Svetlana Ulyanchenko, Pawel J. Schweiger, Edouard B Hannezo, Benjamin D. Simons, and Kim B. Jensen. “Spatiotemporal Switches in Progenitor Cell Fate Govern Upper Hair Follicle Growth and Maintenance.” <i>Journal of Investigative Dermatology</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">https://doi.org/10.1016/j.jid.2025.01.034</a>.","ista":"Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB. 2025. Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance. Journal of Investigative Dermatology. 145(9), 2191–2202.e5."},"article_processing_charge":"No","oa_version":"Published Version","fulldoi":"https://doi.org/10.1016/j.jid.2025.01.034","language":[{"iso":"eng"}],"day":"01","_id":"19507","doi":"10.1016/j.jid.2025.01.034","page":"2191-2202.e5","article_type":"original","issue":"9","isi":1,"has_accepted_license":"1","type":"journal_article","date_published":"2025-09-01T00:00:00Z","month":"09","OA_place":"publisher","file":[{"relation":"main_file","content_type":"application/pdf","file_id":"20874","file_size":7301679,"date_updated":"2025-12-29T14:13:01Z","access_level":"open_access","creator":"dernst","success":1,"date_created":"2025-12-29T14:13:01Z","file_name":"2025_JourInvestigativeDerma_Andersen.pdf","checksum":"a2b313de3cacb53f20f2b91c42612ad9"}],"date_updated":"2025-12-29T14:13:43Z","department":[{"_id":"EdHa"}],"date_created":"2025-04-06T22:01:32Z","acknowledgement":"We thank the members of the Jensen Laboratory for experimental and technical advice, the imaging facilities at reNEW, and animal caretakers for expert assistance. This work was supported by the Lundbeck Foundation (R105-A9755 to KBJ) and the Leo Pharma Foundation (LF-OC-20-000169). The Novo Nordisk Foundation Center for Stem Cell Medicine was supported by a Novo Nordisk Foundation grant (NNF21CC0073729). B.D.S. was supported by the Wellcome Trust (219478/Z/19/Z) and a Royal Society EP Abraham Research Professorship (RP/R1/180165 and RP\\R\\231004). Figure elements were adapted from Bio-Render. KBJ is the lead contact and guarantor of this study.","ddc":["570"],"OA_type":"hybrid","status":"public","quality_controlled":"1","pmid":1,"scopus_import":"1","intvolume":"       145","publication":"Journal of Investigative Dermatology","volume":145,"year":"2025","publisher":"Elsevier","author":[{"last_name":"Andersen","full_name":"Andersen, Marianne S.","first_name":"Marianne S."},{"first_name":"Svetlana","full_name":"Ulyanchenko, Svetlana","last_name":"Ulyanchenko"},{"first_name":"Pawel J.","full_name":"Schweiger, Pawel J.","last_name":"Schweiger"},{"orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"full_name":"Simons, Benjamin D.","first_name":"Benjamin D.","last_name":"Simons"},{"last_name":"Jensen","full_name":"Jensen, Kim B.","first_name":"Kim B."}],"oa":1,"file_date_updated":"2025-12-29T14:13:01Z","abstract":[{"lang":"eng","text":"The epidermis provides a protective barrier against hostile environments. However, our knowledge of how this barrier forms during development and is subsequently maintained remains incomplete. The infundibulum is a cylindrical epidermal tissue compartment that serves as an outlet for hair follicles protruding from the skin and the excretion of the sebaceous glands that are essential for proper skin function. In this study, we applied quantitative fate mapping to address how infundibulum are maintained during adulthood. We demonstrate that progenitors build and maintain tissues through stochastic cell fate choices. Long-term analysis identified a preferential transient contribution from cells initially located at the bottom of the structure to the maintenance of the tissue, with bursts of local progenitor expansion associated with the phases of hair growth. Beyond providing compartment-wide insights into progenitor cell dynamics in infundibulum, these findings demonstrate how spatiotemporal regulation controls transient progenitor dominance."}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"corr_author":"1"},{"has_accepted_license":"1","type":"journal_article","article_type":"original","issue":"4","related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/2025-ig-nobel-prize-for-perfect-pasta-sauce/","description":"News on ISTA"}]},"isi":1,"arxiv":1,"fulldoi":"https://doi.org/10.1063/5.0255841","language":[{"iso":"eng"}],"oa_version":"Published Version","day":"01","_id":"19670","doi":"10.1063/5.0255841","article_processing_charge":"Yes (in subscription journal)","citation":{"chicago":"Bartolucci, G., D. M. Busiello, M. Ciarchi, A. Corticelli, I. Di Terlizzi, Fabrizio Olmeda, D. Revignas, and V. M. Schimmenti. “Phase Behavior of Cacio e Pepe Sauce.” <i>Physics of Fluids</i>. AIP Publishing, 2025. <a href=\"https://doi.org/10.1063/5.0255841\">https://doi.org/10.1063/5.0255841</a>.","ista":"Bartolucci G, Busiello DM, Ciarchi M, Corticelli A, Di Terlizzi I, Olmeda F, Revignas D, Schimmenti VM. 2025. Phase behavior of Cacio e Pepe sauce. Physics of Fluids. 37(4), 044122.","apa":"Bartolucci, G., Busiello, D. M., Ciarchi, M., Corticelli, A., Di Terlizzi, I., Olmeda, F., … Schimmenti, V. M. (2025). Phase behavior of Cacio e Pepe sauce. <i>Physics of Fluids</i>. AIP Publishing. <a href=\"https://doi.org/10.1063/5.0255841\">https://doi.org/10.1063/5.0255841</a>","short":"G. Bartolucci, D.M. Busiello, M. Ciarchi, A. Corticelli, I. Di Terlizzi, F. Olmeda, D. Revignas, V.M. Schimmenti, Physics of Fluids 37 (2025).","ama":"Bartolucci G, Busiello DM, Ciarchi M, et al. Phase behavior of Cacio e Pepe sauce. <i>Physics of Fluids</i>. 2025;37(4). doi:<a href=\"https://doi.org/10.1063/5.0255841\">10.1063/5.0255841</a>","ieee":"G. Bartolucci <i>et al.</i>, “Phase behavior of Cacio e Pepe sauce,” <i>Physics of Fluids</i>, vol. 37, no. 4. AIP Publishing, 2025.","mla":"Bartolucci, G., et al. “Phase Behavior of Cacio e Pepe Sauce.” <i>Physics of Fluids</i>, vol. 37, no. 4, 044122, AIP Publishing, 2025, doi:<a href=\"https://doi.org/10.1063/5.0255841\">10.1063/5.0255841</a>."},"title":"Phase behavior of Cacio e Pepe sauce","publication_identifier":{"eissn":["1089-7666"],"issn":["1070-6631"]},"publication_status":"published","external_id":{"arxiv":["2501.00536"],"isi":["001482986200001"]},"article_number":"044122","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","author":[{"last_name":"Bartolucci","first_name":"G.","full_name":"Bartolucci, G."},{"full_name":"Busiello, D. M.","first_name":"D. M.","last_name":"Busiello"},{"last_name":"Ciarchi","first_name":"M.","full_name":"Ciarchi, M."},{"last_name":"Corticelli","first_name":"A.","full_name":"Corticelli, A."},{"full_name":"Di Terlizzi, I.","first_name":"I.","last_name":"Di Terlizzi"},{"full_name":"Olmeda, Fabrizio","first_name":"Fabrizio","id":"69dbf5fb-8a76-11ed-866b-fb486d8b5689","last_name":"Olmeda"},{"last_name":"Revignas","full_name":"Revignas, D.","first_name":"D."},{"full_name":"Schimmenti, V. M.","first_name":"V. M.","last_name":"Schimmenti"}],"file_date_updated":"2025-05-12T09:31:22Z","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"“Pasta alla Cacio e pepe” is a traditional Italian dish made with pasta, pecorino cheese, and pepper. Despite its simple ingredient list, achieving the perfect texture and creaminess of the sauce can be challenging. In this study, we systematically explore the phase behavior of Cacio e pepe sauce, focusing on its stability at increasing temperatures for various proportions of cheese, water, and starch. We identify starch concentration as the key factor influencing sauce stability, with direct implications for practical cooking. Specifically, we delineate a regime where starch concentrations below 1% (relative to cheese mass) lead to the formation of system-wide clumps, a condition determining what we term the “Mozzarella Phase” and corresponding to an unpleasant and separated sauce. Additionally, we examine the impact of cheese concentration relative to water at a fixed starch level, observing a lower critical solution temperature that we theoretically rationalized by means of a minimal effective free-energy model. We further analyze the effect of a less traditional stabilizer, trisodium citrate, and observe a sharp transition from the Mozzarella Phase to a completely smooth and stable sauce, in contrast to starch-stabilized mixtures, where the transition is more gradual. Finally, we present a scientifically optimized recipe based on our findings, enabling a consistently flawless execution of this classic dish."}],"publisher":"AIP Publishing","publication":"Physics of Fluids","year":"2025","volume":37,"quality_controlled":"1","status":"public","ddc":["530"],"OA_type":"hybrid","scopus_import":"1","intvolume":"        37","department":[{"_id":"EdHa"}],"acknowledgement":"he authors thank Frank Jülicher, for supporting the initiative and stimulating discussions. We thank Tetsuya Spippayashi for enlightening clarifications on the historical origins of Cacio e pepe and Giuseppe Ricchitelli for helping with the construction of the experimental apparatus. We further thank Martina Gaiba, Alessandro Gaiba, John D. Treado, Virginia Lepore, Eleonora Nanu, Julia Kirsch, Lara Koehler, Burak Budanur, Irina Pi-Jaumà, Elizabeth Brückner, M.J. Franco Oñate, Giorgio Nicoletti, and Marco Salvalaglio for their support and for eating up the sample leftovers. Finally, we thank Simone Frau for taking the photograph in Fig. 1(a).","date_created":"2025-05-11T22:02:40Z","file":[{"file_id":"19681","file_size":4926853,"content_type":"application/pdf","relation":"main_file","file_name":"2025_PhysicsFluids_Bartolucci.pdf","success":1,"date_created":"2025-05-12T09:31:22Z","checksum":"242d05898aa0a2348b9c108747adb5ce","creator":"dernst","date_updated":"2025-05-12T09:31:22Z","access_level":"open_access"}],"date_updated":"2026-04-28T13:24:53Z","month":"04","OA_place":"publisher","date_published":"2025-04-01T00:00:00Z"},{"citation":{"short":"S. Jouveshomme, M. Lizée, P. Robin, L. Bocquet, New Journal of Physics 27 (2025).","ama":"Jouveshomme S, Lizée M, Robin P, Bocquet L. Multiple ionic memories in asymmetric nanochannels revealed by mem-spectrometry. <i>New Journal of Physics</i>. 2025;27(6). doi:<a href=\"https://doi.org/10.1088/1367-2630/ade61b\">10.1088/1367-2630/ade61b</a>","ieee":"S. Jouveshomme, M. Lizée, P. Robin, and L. Bocquet, “Multiple ionic memories in asymmetric nanochannels revealed by mem-spectrometry,” <i>New Journal of Physics</i>, vol. 27, no. 6. IOP Publishing, 2025.","mla":"Jouveshomme, Simon, et al. “Multiple Ionic Memories in Asymmetric Nanochannels Revealed by Mem-Spectrometry.” <i>New Journal of Physics</i>, vol. 27, no. 6, 065001, IOP Publishing, 2025, doi:<a href=\"https://doi.org/10.1088/1367-2630/ade61b\">10.1088/1367-2630/ade61b</a>.","apa":"Jouveshomme, S., Lizée, M., Robin, P., &#38; Bocquet, L. (2025). Multiple ionic memories in asymmetric nanochannels revealed by mem-spectrometry. <i>New Journal of Physics</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/1367-2630/ade61b\">https://doi.org/10.1088/1367-2630/ade61b</a>","chicago":"Jouveshomme, Simon, Mathieu Lizée, Paul Robin, and Lydéric Bocquet. “Multiple Ionic Memories in Asymmetric Nanochannels Revealed by Mem-Spectrometry.” <i>New Journal of Physics</i>. IOP Publishing, 2025. <a href=\"https://doi.org/10.1088/1367-2630/ade61b\">https://doi.org/10.1088/1367-2630/ade61b</a>.","ista":"Jouveshomme S, Lizée M, Robin P, Bocquet L. 2025. Multiple ionic memories in asymmetric nanochannels revealed by mem-spectrometry. New Journal of Physics. 27(6), 065001."},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"065001","title":"Multiple ionic memories in asymmetric nanochannels revealed by mem-spectrometry","publication_identifier":{"eissn":["1367-2630"]},"publication_status":"published","external_id":{"isi":["001517731700001"]},"article_type":"original","issue":"6","isi":1,"has_accepted_license":"1","project":[{"name":"IST-BRIDGE: International postdoctoral program","grant_number":"101034413","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","call_identifier":"H2020"}],"type":"journal_article","article_processing_charge":"Yes","oa_version":"Published Version","fulldoi":"https://doi.org/10.1088/1367-2630/ade61b","language":[{"iso":"eng"}],"day":"01","_id":"19966","doi":"10.1088/1367-2630/ade61b","file":[{"success":1,"date_created":"2025-07-08T06:11:59Z","file_name":"2025_NewJourPhysics_Jouveshomme.pdf","checksum":"e0e11aa01c54b20ee6cdd1f6b999571f","date_updated":"2025-07-08T06:11:59Z","access_level":"open_access","creator":"dernst","file_id":"19973","file_size":1296141,"relation":"main_file","content_type":"application/pdf"}],"date_updated":"2025-09-30T13:47:45Z","department":[{"_id":"EdHa"}],"date_created":"2025-07-06T22:01:23Z","acknowledgement":"The authors acknowledge ERC n-AQUA for funding. S J acknowledges CNRS for funding. The authors thank Hummink for pipette supply and characterization. P R acknowledges funding from the European Union Horizon 2020 research and innovation program under the Marie Skodowska-Curie Grant Agreement No. 101034413.","ec_funded":1,"date_published":"2025-06-01T00:00:00Z","month":"06","OA_place":"publisher","publisher":"IOP Publishing","author":[{"last_name":"Jouveshomme","full_name":"Jouveshomme, Simon","first_name":"Simon"},{"first_name":"Mathieu","full_name":"Lizée, Mathieu","last_name":"Lizée"},{"last_name":"Robin","orcid":"0000-0002-5728-9189","first_name":"Paul","id":"48c58128-57b0-11ee-9095-dc28fd97fc1d","full_name":"Robin, Paul"},{"last_name":"Bocquet","first_name":"Lydéric","full_name":"Bocquet, Lydéric"}],"oa":1,"file_date_updated":"2025-07-08T06:11:59Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"Recently discovered nanofluidic memristors, have raised promises for the development of iontronics and neuromorphic computing with ions. Ionic memory effects are related to ion dynamics inside nanochannels, with timescales associated with the manifold physicochemical phenomena occurring at confined interfaces. Here, we explore experimentally the frequency-dependent current–voltage response of model nanochannels—namely glass nanopipettes—to investigate memory effects in ion transport. This characterisation, which we refer to as mem-spectrometry, highlights two characteristic frequencies, associated with short and long timescales of the order of 50 ms and 50 s in the present system. Whereas the former can be associated with ionic diffusion, very long timescales are difficult to explain with conventional transport phenomena. We develop a minimal model accounting for these mem-spectrometry results, pointing to surface charge regulation and ionic adsorption-desorption as possible origins for the long-term memory. Our work demonstrates the relevance of mem-spectrometry to highlight subtle ion transport properties in nanochannels, giving hereby new insights on the mechanisms governing ion transport and current rectification in charged conical nanopores."}],"OA_type":"gold","status":"public","quality_controlled":"1","ddc":["530"],"DOAJ_listed":"1","scopus_import":"1","intvolume":"        27","publication":"New Journal of Physics","volume":27,"year":"2025"},{"abstract":[{"lang":"eng","text":"Theoretical studies have shown that stochasticity can affect the dynamics of ecosystems in counterintuitive ways. However, without knowing the equations governing the dynamics of populations or ecosystems, it is difficult to ascertain the role of stochasticity in real datasets. Therefore, the inverse problem of inferring the governing stochastic equations from datasets is important. Here, we present an equation discovery methodology that takes time series data of state variables as input and outputs a stochastic differential equation. We achieve this by combining traditional approaches from stochastic calculus with the equation discovery techniques. We demonstrate the generality of the method via several applications. First, we deliberately choose various stochastic models with fundamentally different governing equations, yet they produce nearly identical steady-state distributions. We show that we can recover the correct underlying equations, and thus infer the structure of their stability, accurately from the analysis of time series data alone. We demonstrate our method on two real-world datasets—fish schooling and single-cell migration—that have vastly different spatiotemporal scales and dynamics. We illustrate various limitations and potential pitfalls of the method and how to overcome them via diagnostic measures. Finally, we provide our open-source code via a package named PyDaDDy (Python Library for Data-Driven Dynamics)."}],"author":[{"last_name":"Nabeel","first_name":"Arshed","full_name":"Nabeel, Arshed"},{"last_name":"Karichannavar","first_name":"Ashwin","full_name":"Karichannavar, Ashwin"},{"full_name":"Palathingal, Shuaib","first_name":"Shuaib","last_name":"Palathingal"},{"first_name":"Jitesh","full_name":"Jhawar, Jitesh","last_name":"Jhawar"},{"last_name":"Brückner","full_name":"Brückner, David","first_name":"David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","orcid":"0000-0001-7205-2975"},{"full_name":"Raj M, Danny","first_name":"Danny","last_name":"Raj M"},{"full_name":"Guttal, Vishwesha","first_name":"Vishwesha","last_name":"Guttal"}],"oa":1,"publisher":"University of Chicago Press","publication":"The American Naturalist","volume":205,"year":"2025","intvolume":"       205","status":"public","quality_controlled":"1","OA_type":"green","pmid":1,"acknowledgement":"V.G. acknowledges support from the Science and Engi-neering Research Board, Department of Biotechnology,and the Indo-French Centre for the Promotion of Ad-vanced Research (64T4-1). D.R.M. acknowledges supportfrom a Department of Science and Technology (DST) In-novation in Science Pursuit for Inspired Research (IN-SPIRE) Faculty Award. J.J. acknowledges support froma Humboldt postdoctoral fellowship and the Heidelber-ger Akademie der Wissenschaften, Heidelberg, Germany.D.B.B. acknowledges support from the NOMIS Founda-tion and an European Molecular Biology Organization(EMBO) postdoctoral fellowship (ALTF 343-2022). A.N.and S.P. acknowledge support from Ministry of Educa-tion (MoE) PhD fellowships. We thank Ashrit Mangal-wedhekar, Vivek Jadhav, Shikhara Bhat, Cassandre Aimon,and Harishankar Muppirala for comments on the manu-script and code. We thank Kollegala Sharma for his inputon the Kannada translation of the title and abstract.Data-Driven Model Discovery E115","date_created":"2025-07-21T08:37:27Z","department":[{"_id":"EdHa"}],"date_updated":"2025-09-30T14:14:43Z","OA_place":"repository","month":"04","date_published":"2025-04-01T00:00:00Z","project":[{"grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b"}],"type":"journal_article","issue":"4","related_material":{"record":[{"status":"public","id":"20121","relation":"software"}]},"isi":1,"arxiv":1,"page":"E100-E117","article_type":"original","doi":"10.1086/734083","oa_version":"Preprint","fulldoi":"https://doi.org/10.1086/734083","language":[{"iso":"eng"}],"_id":"20056","day":"01","article_processing_charge":"No","citation":{"apa":"Nabeel, A., Karichannavar, A., Palathingal, S., Jhawar, J., Brückner, D., Raj M, D., &#38; Guttal, V. (2025). Discovering stochastic dynamical equations from ecological time series data. <i>The American Naturalist</i>. University of Chicago Press. <a href=\"https://doi.org/10.1086/734083\">https://doi.org/10.1086/734083</a>","short":"A. Nabeel, A. Karichannavar, S. Palathingal, J. Jhawar, D. Brückner, D. Raj M, V. Guttal, The American Naturalist 205 (2025) E100–E117.","ama":"Nabeel A, Karichannavar A, Palathingal S, et al. Discovering stochastic dynamical equations from ecological time series data. <i>The American Naturalist</i>. 2025;205(4):E100-E117. doi:<a href=\"https://doi.org/10.1086/734083\">10.1086/734083</a>","ieee":"A. Nabeel <i>et al.</i>, “Discovering stochastic dynamical equations from ecological time series data,” <i>The American Naturalist</i>, vol. 205, no. 4. University of Chicago Press, pp. E100–E117, 2025.","mla":"Nabeel, Arshed, et al. “Discovering Stochastic Dynamical Equations from Ecological Time Series Data.” <i>The American Naturalist</i>, vol. 205, no. 4, University of Chicago Press, 2025, pp. E100–17, doi:<a href=\"https://doi.org/10.1086/734083\">10.1086/734083</a>.","chicago":"Nabeel, Arshed, Ashwin Karichannavar, Shuaib Palathingal, Jitesh Jhawar, David Brückner, Danny Raj M, and Vishwesha Guttal. “Discovering Stochastic Dynamical Equations from Ecological Time Series Data.” <i>The American Naturalist</i>. University of Chicago Press, 2025. <a href=\"https://doi.org/10.1086/734083\">https://doi.org/10.1086/734083</a>.","ista":"Nabeel A, Karichannavar A, Palathingal S, Jhawar J, Brückner D, Raj M D, Guttal V. 2025. Discovering stochastic dynamical equations from ecological time series data. The American Naturalist. 205(4), E100–E117."},"main_file_link":[{"url":"https://doi.org/10.48550/arXiv.2205.02645","open_access":"1"}],"publication_identifier":{"eissn":["1537-5323"],"issn":["0003-0147"]},"publication_status":"published","external_id":{"pmid":["40179429"],"arxiv":["2205.02645"],"isi":["001433250500001"]},"title":"Discovering stochastic dynamical equations from ecological time series data","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"APC_amount":"3642,79 EUR","volume":16,"year":"2025","publication":"Frontiers in Plant Science","intvolume":"        16","scopus_import":"1","pmid":1,"DOAJ_listed":"1","PlanS_conform":"1","quality_controlled":"1","status":"public","ddc":["580"],"OA_type":"gold","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"Introduction: Acid-growth theory has been postulated in the 70s to explain the rapid elongation of plant cells in response to the hormone auxin. More recently, it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting acidification of the apoplast is the principal mechanism by which auxin and other hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances, the impact of this acidification on the mechanical properties of the cell wall remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate hormone-induced tissue elongation and local changes in cell wall mechanical properties. Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase (PME), along with calcium chelators, we investigate the effect of pectin modification in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification of apoplast is necessary and sufficient to induce cell elongation through promoting cell wall softening. Moreover, we show that enhanced PME activity can induce both cell wall softening or stiffening in extracellular calcium dependent-manner and that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion: Our results confirm a dual role of PME in plant cell elongation. However, further investigation is needed to assess the status of pectin following short- or long-term PME treatments in order to determine if pectin methyl-esterification might promote its degradation as well as the role of PME inhibitors upon PME induction."}],"corr_author":"1","oa":1,"file_date_updated":"2025-07-31T07:28:54Z","author":[{"last_name":"Gallemi","orcid":"0000-0003-4675-6893","id":"460C6802-F248-11E8-B48F-1D18A9856A87","first_name":"Marçal","full_name":"Gallemi, Marçal"},{"orcid":"0000-0001-9179-6099","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C","full_name":"Montesinos López, Juan C","last_name":"Montesinos López"},{"first_name":"Nikola","id":"18e95355-e05a-11ea-a9c0-8fba1b89e83a","full_name":"Zarevski, Nikola","last_name":"Zarevski"},{"last_name":"Pribyl","first_name":"Jan","full_name":"Pribyl, Jan"},{"full_name":"Skládal, Petr","first_name":"Petr","last_name":"Skládal"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"last_name":"Benková","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","full_name":"Benková, Eva"}],"publisher":"Frontiers Media","OA_place":"publisher","month":"07","date_published":"2025-07-04T00:00:00Z","ec_funded":1,"date_created":"2025-07-27T22:01:26Z","acknowledgement":"The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127). We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler for the efforts to establish immunodetection method.","department":[{"_id":"EdHa"},{"_id":"EvBe"},{"_id":"CaGu"}],"date_updated":"2026-05-20T07:53:03Z","file":[{"relation":"main_file","content_type":"application/pdf","file_size":3665187,"file_id":"20093","access_level":"open_access","date_updated":"2025-07-31T07:28:54Z","creator":"dernst","checksum":"9e6b8b53ba56d4a24a9bd91cf6d2dc58","file_name":"2025_FrontiersPlantSc_Gallemi.pdf","date_created":"2025-07-31T07:28:54Z","success":1}],"doi":"10.3389/fpls.2025.1612366","day":"04","_id":"20080","oa_version":"Published Version","fulldoi":"https://doi.org/10.3389/fpls.2025.1612366","language":[{"iso":"eng"}],"article_processing_charge":"Yes","type":"journal_article","has_accepted_license":"1","project":[{"_id":"253FCA6A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis"},{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"}],"isi":1,"article_type":"original","external_id":{"isi":["001530690900001"],"pmid":["40688689"]},"publication_identifier":{"eissn":["1664-462X"]},"publication_status":"published","title":"Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_number":"1612366","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"}],"citation":{"chicago":"Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl, Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>. Frontiers Media, 2025. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>.","ista":"Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB, Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.","apa":"Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P., Hannezo, E. B., &#38; Benková, E. (2025). Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>","short":"M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B. Hannezo, E. Benková, Frontiers in Plant Science 16 (2025).","ama":"Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. 2025;16. doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>","mla":"Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>, vol. 16, 1612366, Frontiers Media, 2025, doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>.","ieee":"M. Gallemi <i>et al.</i>, “Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties,” <i>Frontiers in Plant Science</i>, vol. 16. Frontiers Media, 2025."}},{"date_updated":"2025-12-30T09:34:11Z","department":[{"_id":"EdHa"}],"date_created":"2025-08-31T22:01:33Z","acknowledgement":"We are grateful to members of S.G.’s laboratory for feedback and suggestions. We thank E. Hannezo, J. O. Rädler, M. Piel, O. du Roure and J. Heuvingh for inspiring discussions. Y.K. and S.G. acknowledge J. B. Braquenier from Nikon Instruments Belux and the Nikon BioImaging Lab in Leiden (the Netherlands) for their support with the Nikon Spatial Array Confocal enhanced-resolution confocal microscopy. We thank D. S. Herrador and M. Balland for their help in improving the microprinting method. D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). Y.K., M.L. and S.G. acknowledge funding from the University of Mons (FEDER Prostem Research Project no. 1510614, Wallonia DG06), the F.R.S.-FNRS (Epiforce Project no. T.0092.21, Cellsqueezer Project no. J.0061.23 and Optopattern Project no. U.NO26.22) and the Interreg projects ANTIRESI and MICROPLAITE, which are financially supported by Interreg France-Wallonie-Vlaanderen (Fonds Européen de Développement Régional). Y.K. and M.L. are financially supported by F.R.S.-FNRS as FRIA Grantee FNRS and Postdoctoral Fellow (Chargé de Recherches), respectively. Y.K. and S.G. acknowledge le Fonds pour la Recherche Médicale dans le Hainaut (FRMH). G.C. was supported by a grant from the Biotechnology and Biological Sciences Research Council (grant no. BB/V007483/1).","date_published":"2025-09-01T00:00:00Z","month":"09","publisher":"Springer Nature","author":[{"last_name":"Kalukula","first_name":"Yohalie","full_name":"Kalukula, Yohalie"},{"full_name":"Luciano, Marine","first_name":"Marine","last_name":"Luciano"},{"last_name":"Simanov","full_name":"Simanov, Gleb","first_name":"Gleb"},{"first_name":"Guillaume","full_name":"Charras, Guillaume","last_name":"Charras"},{"orcid":"0000-0001-7205-2975","id":"e1e86031-6537-11eb-953a-f7ab92be508d","first_name":"David","full_name":"Brückner, David","last_name":"Brückner"},{"first_name":"Sylvain","full_name":"Gabriele, Sylvain","last_name":"Gabriele"}],"abstract":[{"lang":"eng","text":"Cell migration in narrow microenvironments occurs in numerous physiological processes. It involves successive cycles of confinement and release that drive important morphological changes. However, it remains unclear whether migrating cells can retain a memory of their past morphological states that could potentially facilitate their navigation through confined spaces. We demonstrate that local geometry governs a switch between two cell morphologies, thereby facilitating cell passage through long and narrow gaps. We combined cell migration assays on standardized microsystems with biophysical modelling and biochemical perturbations to show that migrating cells have a long-term memory of past confinement events. The morphological cell states correlate across transitions through actin cortex remodelling. These findings indicate that mechanical memory in migrating cells plays an active role in their migratory potential in confined environments."}],"corr_author":"1","quality_controlled":"1","OA_type":"closed access","status":"public","scopus_import":"1","intvolume":"        21","publication":"Nature Physics","volume":21,"year":"2025","citation":{"ista":"Kalukula Y, Luciano M, Simanov G, Charras G, Brückner D, Gabriele S. 2025. The actin cortex acts as a mechanical memory of morphology in confined migrating cells. Nature Physics. 21, 1451–1461.","chicago":"Kalukula, Yohalie, Marine Luciano, Gleb Simanov, Guillaume Charras, David Brückner, and Sylvain Gabriele. “The Actin Cortex Acts as a Mechanical Memory of Morphology in Confined Migrating Cells.” <i>Nature Physics</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41567-025-02980-z\">https://doi.org/10.1038/s41567-025-02980-z</a>.","mla":"Kalukula, Yohalie, et al. “The Actin Cortex Acts as a Mechanical Memory of Morphology in Confined Migrating Cells.” <i>Nature Physics</i>, vol. 21, Springer Nature, 2025, pp. 1451–61, doi:<a href=\"https://doi.org/10.1038/s41567-025-02980-z\">10.1038/s41567-025-02980-z</a>.","ieee":"Y. Kalukula, M. Luciano, G. Simanov, G. Charras, D. Brückner, and S. Gabriele, “The actin cortex acts as a mechanical memory of morphology in confined migrating cells,” <i>Nature Physics</i>, vol. 21. Springer Nature, pp. 1451–1461, 2025.","ama":"Kalukula Y, Luciano M, Simanov G, Charras G, Brückner D, Gabriele S. The actin cortex acts as a mechanical memory of morphology in confined migrating cells. <i>Nature Physics</i>. 2025;21:1451-1461. doi:<a href=\"https://doi.org/10.1038/s41567-025-02980-z\">10.1038/s41567-025-02980-z</a>","short":"Y. Kalukula, M. Luciano, G. Simanov, G. Charras, D. Brückner, S. Gabriele, Nature Physics 21 (2025) 1451–1461.","apa":"Kalukula, Y., Luciano, M., Simanov, G., Charras, G., Brückner, D., &#38; Gabriele, S. (2025). The actin cortex acts as a mechanical memory of morphology in confined migrating cells. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-02980-z\">https://doi.org/10.1038/s41567-025-02980-z</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"The actin cortex acts as a mechanical memory of morphology in confined migrating cells","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"publication_status":"published","external_id":{"isi":["001556019400001"]},"page":"1451-1461","article_type":"original","isi":1,"project":[{"grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b"}],"type":"journal_article","article_processing_charge":"No","oa_version":"None","fulldoi":"https://doi.org/10.1038/s41567-025-02980-z","language":[{"iso":"eng"}],"_id":"20259","day":"01","doi":"10.1038/s41567-025-02980-z"},{"publisher":"National Academy of Sciences","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"Cell and tissue movement in development, cancer invasion, and immune response relies on chemical or mechanical guidance cues. In many systems, this behavior is locally directed by self-generated signaling gradients rather than long-range, prepatterned cues. However, how heterogeneous mixtures of cells interact nonreciprocally and navigate through self-generated gradients remains largely unexplored. Here, we introduce a theoretical framework for the self-organized chemotaxis of heterogeneous cell populations. We find that the relative chemotactic sensitivities of different cell populations control their long-time coupling and comigration dynamics, with boundary conditions such as external cell and attractant reservoirs substantially influencing the migration patterns. Our model predicts an optimal parameter regime that enables robust and colocalized migration. We test our theoretical predictions with in vitro experiments demonstrating the comigration of distinct immune cell populations, and quantitatively reproduce observed migration patterns under wild-type and perturbed conditions. Interestingly, immune cell comigration occurs close to the predicted optimal regime. Finally, we incorporate mechanical interactions into our framework, revealing a nontrivial interplay between chemotactic and mechanical nonreciprocity in driving collective migration. Together, our findings suggest that self-generated chemotaxis is a robust strategy for the navigation of mixed cell populations."}],"corr_author":"1","author":[{"id":"50B2A802-6007-11E9-A42B-EB23E6697425","first_name":"Mehmet C","orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C","last_name":"Ucar"},{"last_name":"Zane","full_name":"Zane, Alsberga","orcid":"0009-0003-0415-7603","first_name":"Alsberga","id":"60f7509a-f652-11ea-9d86-b963d6490d7c"},{"last_name":"Alanko","orcid":"0000-0002-7698-3061","first_name":"Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","full_name":"Alanko, Jonna H"},{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt"},{"last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"}],"oa":1,"file_date_updated":"2025-09-08T07:23:29Z","intvolume":"       122","OA_type":"hybrid","quality_controlled":"1","status":"public","ddc":["570"],"PlanS_conform":"1","pmid":1,"scopus_import":"1","APC_amount":"5766,07 EUR","publication":"Proceedings of the National Academy of Sciences","year":"2025","volume":122,"date_updated":"2026-05-20T08:59:54Z","file":[{"file_id":"20307","file_size":16069140,"relation":"main_file","content_type":"application/pdf","success":1,"date_created":"2025-09-08T07:23:29Z","file_name":"2025_PNAS_Ucar.pdf","checksum":"b36abd92673b6d76376fc9434bad52cc","creator":"dernst","date_updated":"2025-09-08T07:23:29Z","access_level":"open_access"}],"date_created":"2025-09-07T22:01:32Z","acknowledgement":"We thank all members of the M.S. and E.H. groups for stimulating discussions.We thank the Imaging and Optics facility, the Pre-clinical and Lab Support facility of the Institute of Science and Technology Austria for their excellent support and provided resources for the experimental research. In particular, we thank Jack Merrin from the Nanofabrication facility who generated the microfabricated channel used in this study. This work received funding fromt he European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 851288 to E.H.). M.C.U.is funded by a University of Sheffield Strategic Research Fellowship in the Physics of Life and Quantitative Biology.","ec_funded":1,"department":[{"_id":"EdHa"},{"_id":"MiSi"}],"date_published":"2025-08-26T00:00:00Z","OA_place":"publisher","month":"08","issue":"34","related_material":{"link":[{"url":"https://github.com/mehmetcanucar/Self-generated-chemotaxis","relation":"software"}]},"isi":1,"article_type":"original","has_accepted_license":"1","project":[{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E"}],"type":"journal_article","article_processing_charge":"Yes (in subscription journal)","doi":"10.1073/pnas.2504064122","fulldoi":"https://doi.org/10.1073/pnas.2504064122","oa_version":"Published Version","language":[{"iso":"eng"}],"_id":"20289","day":"26","citation":{"mla":"Ucar, Mehmet C., et al. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34, e2504064122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>.","ieee":"M. C. Ucar, A. Zane, J. H. Alanko, M. K. Sixt, and E. B. Hannezo, “Self-generated chemotaxis of mixed cell populations,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34. National Academy of Sciences, 2025.","ama":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(34). doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>","short":"M.C. Ucar, A. Zane, J.H. Alanko, M.K. Sixt, E.B. Hannezo, Proceedings of the National Academy of Sciences 122 (2025).","apa":"Ucar, M. C., Zane, A., Alanko, J. H., Sixt, M. K., &#38; Hannezo, E. B. (2025). Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>","ista":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. 2025. Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. 122(34), e2504064122.","chicago":"Ucar, Mehmet C, Alsberga Zane, Jonna H Alanko, Michael K Sixt, and Edouard B Hannezo. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>."},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"NanoFab"}],"article_number":"e2504064122","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"publication_status":"published","external_id":{"isi":["001562181600001"],"pmid":["40838890"]},"title":"Self-generated chemotaxis of mixed cell populations"},{"_id":"20424","day":"26","fulldoi":"https://doi.org/10.1038/s41467-025-62882-9","language":[{"iso":"eng"}],"oa_version":"Published Version","doi":"10.1038/s41467-025-62882-9","article_processing_charge":"Yes","type":"journal_article","project":[{"_id":"628f3fb1-2b32-11ec-9570-83ce778803f7","grant_number":"ALTF 522-2021","name":"Biomechanics of stem cell fate determination"},{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis"}],"has_accepted_license":"1","article_type":"original","isi":1,"title":"Mechanical control of cell fate decisions in the skin epidermis","external_id":{"pmid":["41006218"],"isi":["001582555200011"]},"publication_identifier":{"eissn":["2041-1723"]},"publication_status":"published","article_number":"8440","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledged_ssus":[{"_id":"Bio"}],"citation":{"apa":"Sahu, P., Monteiro-Ferreira, S., Canato, S., Soares, R. M., Sánchez-Danés, A., &#38; Hannezo, E. B. (2025). Mechanical control of cell fate decisions in the skin epidermis. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-62882-9\">https://doi.org/10.1038/s41467-025-62882-9</a>","ama":"Sahu P, Monteiro-Ferreira S, Canato S, Soares RM, Sánchez-Danés A, Hannezo EB. Mechanical control of cell fate decisions in the skin epidermis. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-62882-9\">10.1038/s41467-025-62882-9</a>","short":"P. Sahu, S. Monteiro-Ferreira, S. Canato, R.M. Soares, A. Sánchez-Danés, E.B. Hannezo, Nature Communications 16 (2025).","ieee":"P. Sahu, S. Monteiro-Ferreira, S. Canato, R. M. Soares, A. Sánchez-Danés, and E. B. Hannezo, “Mechanical control of cell fate decisions in the skin epidermis,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","mla":"Sahu, Preeti, et al. “Mechanical Control of Cell Fate Decisions in the Skin Epidermis.” <i>Nature Communications</i>, vol. 16, 8440, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-62882-9\">10.1038/s41467-025-62882-9</a>.","chicago":"Sahu, Preeti, Sara Monteiro-Ferreira, Sara Canato, Raquel Maia Soares, Adriana Sánchez-Danés, and Edouard B Hannezo. “Mechanical Control of Cell Fate Decisions in the Skin Epidermis.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-62882-9\">https://doi.org/10.1038/s41467-025-62882-9</a>.","ista":"Sahu P, Monteiro-Ferreira S, Canato S, Soares RM, Sánchez-Danés A, Hannezo EB. 2025. Mechanical control of cell fate decisions in the skin epidermis. Nature Communications. 16, 8440."},"year":"2025","volume":16,"publication":"Nature Communications","APC_amount":"7068 EUR","DOAJ_listed":"1","pmid":1,"scopus_import":"1","status":"public","OA_type":"gold","ddc":["570"],"quality_controlled":"1","intvolume":"        16","file_date_updated":"2025-10-13T12:37:04Z","oa":1,"author":[{"id":"55BA52EE-A185-11EA-88FD-18AD3DDC885E","first_name":"Preeti","full_name":"Sahu, Preeti","last_name":"Sahu"},{"full_name":"Monteiro-Ferreira, Sara","first_name":"Sara","last_name":"Monteiro-Ferreira"},{"full_name":"Canato, Sara","first_name":"Sara","last_name":"Canato"},{"full_name":"Soares, Raquel Maia","first_name":"Raquel Maia","last_name":"Soares"},{"first_name":"Adriana","full_name":"Sánchez-Danés, Adriana","last_name":"Sánchez-Danés"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"}],"corr_author":"1","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"abstract":[{"text":"Homeostasis relies on a precise balance of fate choices between renewal and differentiation. Although progress has been done to characterize the dynamics of single-cell fate choices, their underlying mechanistic basis often remains unclear. Concentrating on skin epidermis as a paradigm for multilayered tissues with complex fate choices, we develop a 3D vertex-based model with proliferation in the basal layer, showing that mechanical competition for space naturally gives rise to homeostasis and neutral drift dynamics that are seen experimentally. We then explore the effect of introducing mechanical heterogeneities between cellular subpopulations. We uncover that relatively small tension heterogeneities, reflected by distinct morphological changes in single-cell shapes, can be sufficient to heavily tilt cellular dynamics towards exponential growth. We thus derive a master relationship between cell shape and long-term clonal dynamics, which we validated during basal cell carcinoma initiation in mouse epidermis. Altogether, we propose a theoretical framework to link mechanical forces, quantitative cellular morphologies and cellular fate outcomes in complex tissues.","lang":"eng"}],"publisher":"Springer Nature","month":"09","OA_place":"publisher","date_published":"2025-09-26T00:00:00Z","department":[{"_id":"EdHa"}],"ec_funded":1,"date_created":"2025-10-05T22:01:34Z","acknowledgement":"We thank Alois Schlögl, Paula Sanematsu, Susana Moreno Flores, Bernat Corominas-Murtra, Stefania Tavano, Gayathri Singharaju, and Hannezo group members for helpful discussions, the Bioimaging facility at ISTA, as well as Matthias Merkel and Lisa Manning for sharing the 3D Voronoi code. We also thank the Champalimaud animal facility, Anna Pezzarossa and the Champalimaud ABBE platform for the help with microscopy and image processing. This work was supported by EMBO (ALTF 522-2021), a Fundação para a Ciência e Tecnologia grant to A.S.D. (PTDC/MED-ONC/5553/2020), as well as the European Research Council (grant 851288 to EH). A.S.D., S.C., and R.M.S. are supported by QuantOCancer Project Horizon European Union’s Horizon 2020 program (grant agreement No 810653).","file":[{"file_id":"20464","file_size":2816813,"content_type":"application/pdf","relation":"main_file","file_name":"2025_NatureComm_Sahu.pdf","date_created":"2025-10-13T12:37:04Z","success":1,"checksum":"d1656576883b23902545328e2d640234","creator":"dernst","date_updated":"2025-10-13T12:37:04Z","access_level":"open_access"}],"date_updated":"2026-05-20T08:52:01Z"},{"date_updated":"2026-01-05T14:26:28Z","department":[{"_id":"EdHa"}],"date_created":"2025-10-05T22:01:36Z","acknowledgement":"We thank all the members of our groups for discussions and support. We thank A. Menéndez, S. Usieto, M. Purciolas and E. Coderch for technical assistance. We thank G. Charras (London Centre for Nanotechnology, UK) and M. Sheetz (Columbia University, USA) for sharing cells used in this work. We thank J. Ivaska (University of Turku, Finland) for sharing integrin α5-GFP DNA plasmid. We thank P. Guillamat for technical advice and A. Labernardie for providing the microfluidic channels. We thank M. Gómez-González for sharing the 2D traction microscopy algorithm. Finally, we thank P. Guillamat, J. Abenza, G. Ceada, L. Faure, E. Dalaka, M. Matejčić, A. Beedle, I. Granero, O. Baguer, A. Albajar and N. Chahare for discussions. This paper was funded by the Generalitat de Catalunya (Grant Nos. AGAUR SGR-2017-01602 to X.T. and 2021 SGR 00523 to R.S. and the CERCA Programme and ICREA Academia awards to P.R.-C.), the Spanish Ministry for Science and Innovation MICCINN/FEDER (Grant Nos. PID2021-128635NB-I00, MCIN/AEI/10.13039/501100011033 and ERDF-EU A way of making Europe to X.T., PID2021-128674OB-I00 and CNS2022-135533 to R.S. and PID2019-110298GB-I00 to P.R.-C.), the European Research Council (Grant Nos. 101097753 to P.R.-C. and Adv-883739 to X.T.), Fundació la Marató de TV3 (Project Award 201903-30-31-32 to X.T.), the European Commission (Grant No. H2020-FETPROACT-01-2016-731957 to P.R.-C. and X.T.) and La Caixa Foundation (Grant No. LCF/PR/HR20/52400004 to P.R.-C. and X.T.). R.S. is a Serra-Hunter fellow. D.B.B. was supported by the NOMIS foundation as a NOMIS fellow, by the European Molecular Biology Organization (Postdoctoral Fellowship ALTF 343-2022) and by the Austrian Academy of Sciences through an APART-MINT Fellowship. I.C.F. acknowledges support from the European Foundation for the Study of Chronic Liver Failure. IBEC is recipient of a Severo Ochoa Award of Excellence from MINECO.","date_published":"2025-10-01T00:00:00Z","month":"10","OA_place":"repository","publisher":"Springer Nature","oa":1,"author":[{"last_name":"Fortunato","full_name":"Fortunato, Isabela Corina","first_name":"Isabela Corina"},{"last_name":"Brückner","orcid":"0000-0001-7205-2975","first_name":"David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","full_name":"Brückner, David"},{"first_name":"Steffen","full_name":"Grosser, Steffen","last_name":"Grosser"},{"first_name":"Rohit","full_name":"Nautiyal, Rohit","last_name":"Nautiyal"},{"first_name":"Leone","full_name":"Rossetti, Leone","last_name":"Rossetti"},{"full_name":"Bosch-Padrós, Miquel","first_name":"Miquel","last_name":"Bosch-Padrós"},{"first_name":"Jonel","full_name":"Trebicka, Jonel","last_name":"Trebicka"},{"first_name":"Pere","full_name":"Roca-Cusachs, Pere","last_name":"Roca-Cusachs"},{"full_name":"Sunyer, Raimon","first_name":"Raimon","last_name":"Sunyer"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Xavier","full_name":"Trepat, Xavier","last_name":"Trepat"}],"corr_author":"1","abstract":[{"lang":"eng","text":"Haptotaxis is the process of directed cell migration along gradients of extracellular matrix density and is central to morphogenesis, immune responses and cancer invasion. It is commonly assumed that cells respond to these gradients by migrating directionally towards the regions of highest ligand density. In contrast with this view, here we show that cells exposed to micropatterned fibronectin gradients exhibit a wide range of complex trajectories, including directed haptotactic migration up the gradient but also linear oscillations and circles with extended periods of migration down the gradient. To explain this behaviour, we developed a biophysical model of haptotactic cell migration based on a coarse-grained molecular clutch model coupled to persistent stochastic polarity dynamics. Although initial haptotactic migration is explained by the differential friction at the front and back of the cell, the observed complex trajectories over longer timescales arise from the interplay between differential friction, persistence and physical confinement. Overall, our study reveals that confinement and persistence modulate the ability of cells to sense and respond to haptotactic cues and provides a framework for understanding how cells navigate complex environments."}],"scopus_import":"1","status":"public","OA_type":"green","quality_controlled":"1","intvolume":"        21","year":"2025","volume":21,"publication":"Nature Physics","main_file_link":[{"url":"https://doi.org/10.1101/2024.12.02.626413","open_access":"1"}],"citation":{"ieee":"I. C. Fortunato <i>et al.</i>, “Single-cell migration along and against confined haptotactic gradients,” <i>Nature Physics</i>, vol. 21. Springer Nature, pp. 1638–1647, 2025.","mla":"Fortunato, Isabela Corina, et al. “Single-Cell Migration along and against Confined Haptotactic Gradients.” <i>Nature Physics</i>, vol. 21, Springer Nature, 2025, pp. 1638–47, doi:<a href=\"https://doi.org/10.1038/s41567-025-03015-3\">10.1038/s41567-025-03015-3</a>.","short":"I.C. Fortunato, D. Brückner, S. Grosser, R. Nautiyal, L. Rossetti, M. Bosch-Padrós, J. Trebicka, P. Roca-Cusachs, R. Sunyer, E.B. Hannezo, X. Trepat, Nature Physics 21 (2025) 1638–1647.","ama":"Fortunato IC, Brückner D, Grosser S, et al. Single-cell migration along and against confined haptotactic gradients. <i>Nature Physics</i>. 2025;21:1638-1647. doi:<a href=\"https://doi.org/10.1038/s41567-025-03015-3\">10.1038/s41567-025-03015-3</a>","apa":"Fortunato, I. C., Brückner, D., Grosser, S., Nautiyal, R., Rossetti, L., Bosch-Padrós, M., … Trepat, X. (2025). Single-cell migration along and against confined haptotactic gradients. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-03015-3\">https://doi.org/10.1038/s41567-025-03015-3</a>","ista":"Fortunato IC, Brückner D, Grosser S, Nautiyal R, Rossetti L, Bosch-Padrós M, Trebicka J, Roca-Cusachs P, Sunyer R, Hannezo EB, Trepat X. 2025. Single-cell migration along and against confined haptotactic gradients. Nature Physics. 21, 1638–1647.","chicago":"Fortunato, Isabela Corina, David Brückner, Steffen Grosser, Rohit Nautiyal, Leone Rossetti, Miquel Bosch-Padrós, Jonel Trebicka, et al. “Single-Cell Migration along and against Confined Haptotactic Gradients.” <i>Nature Physics</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41567-025-03015-3\">https://doi.org/10.1038/s41567-025-03015-3</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Single-cell migration along and against confined haptotactic gradients","external_id":{"isi":["001581659900001"]},"publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"publication_status":"published","article_type":"original","page":"1638-1647","isi":1,"type":"journal_article","project":[{"_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development"}],"article_processing_charge":"No","day":"01","_id":"20431","fulldoi":"https://doi.org/10.1038/s41567-025-03015-3","oa_version":"Preprint","language":[{"iso":"eng"}],"doi":"10.1038/s41567-025-03015-3"},{"publication":"Physical Review X","volume":15,"year":"2025","APC_amount":"4695,11 EUR","ddc":["530"],"OA_type":"gold","quality_controlled":"1","status":"public","DOAJ_listed":"1","PlanS_conform":"1","scopus_import":"1","intvolume":"        15","author":[{"last_name":"Martinet","id":"b37485a8-d343-11eb-a0e9-df8c484ef8ab","first_name":"Quentin","orcid":"0000-0002-2916-6632","full_name":"Martinet, Quentin"},{"first_name":"Yuting I","id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","full_name":"Li, Yuting I","last_name":"Li"},{"full_name":"Aubret, A.","first_name":"A.","last_name":"Aubret"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo"},{"last_name":"Palacci","full_name":"Palacci, Jérémie A","orcid":"0000-0002-7253-9465","id":"8fb92548-2b22-11eb-b7c1-a3f0d08d7c7d","first_name":"Jérémie A"}],"file_date_updated":"2025-12-01T07:30:00Z","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"abstract":[{"lang":"eng","text":"In equilibrium, the physical properties of matter are set by the interactions between the constituents. In contrast, the energy input of the individual components controls the behavior of synthetic or living active matter. Great progress has been made in understanding the emergent phenomena in active fluids, though their inability to resist shear forces hinders their practical use. This motivates the exploration of active solids as shape-shifting materials, yet, we lack controlled synthetic systems to devise active solids with unconventional properties. Here we build active elastic beams from dozens of active colloids and unveil complex emergent behaviors such as self-oscillations or persistent rotations. Developing tensile tests at the microscale, we show that the active beams are ultrasoft materials, with large (nonequilibrium) fluctuations. Combining experiments, theory, and stochastic inference, we show that the dynamics of the active beams can be mapped on different phase transitions which are tuned by boundary conditions. More quantitatively, we assess all relevant parameters by independent measurements or first-principles calculations, and find that our theoretical description agrees with the experimental observations. Our results demonstrate that the simple addition of activity to an elastic beam unveils novel physics and can inspire design strategies for active solids and functional microscopic machines."}],"corr_author":"1","publisher":"American Physical Society","month":"10","OA_place":"publisher","date_published":"2025-10-31T00:00:00Z","department":[{"_id":"EdHa"},{"_id":"JePa"}],"acknowledgement":"The authors thank Andela Saric, Christoph Zechner, and Paul Robin for helpful discussions. J. P. acknowledges support by ERC grant (VULCAN, 101086998) and U.S. ARO under Award No. W911NF2310008. Y. I. L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 101034413.","date_created":"2025-11-30T23:02:08Z","ec_funded":1,"file":[{"checksum":"bb64ea9f2c400205fd89e9bdd15cc850","date_created":"2025-12-01T07:30:00Z","file_name":"2025_PhysicalReviewX_Martinet.pdf","success":1,"access_level":"open_access","date_updated":"2025-12-01T07:30:00Z","creator":"dernst","file_size":5902259,"file_id":"20714","relation":"main_file","content_type":"application/pdf"}],"date_updated":"2026-05-20T08:58:06Z","fulldoi":"https://doi.org/10.1103/rjk2-q2wh","oa_version":"Published Version","language":[{"iso":"eng"}],"day":"31","_id":"20708","doi":"10.1103/rjk2-q2wh","article_processing_charge":"Yes","has_accepted_license":"1","project":[{"_id":"bdac72da-d553-11ed-ba76-eae56e802b74","grant_number":"101086998","name":"VULCAN: matter, powered from within"},{"grant_number":"101034413","name":"IST-BRIDGE: International postdoctoral program","call_identifier":"H2020","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c"}],"type":"journal_article","article_type":"original","issue":"4","arxiv":1,"title":"Emergent dynamics of active elastic microbeams","publication_identifier":{"eissn":["2160-3308"]},"publication_status":"published","external_id":{"arxiv":["2508.20642"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_number":"041017","citation":{"ista":"Martinet Q, Li YI, Aubret A, Hannezo EB, Palacci JA. 2025. Emergent dynamics of active elastic microbeams. Physical Review X. 15(4), 041017.","chicago":"Martinet, Quentin, Yuting I Li, A. Aubret, Edouard B Hannezo, and Jérémie A Palacci. “Emergent Dynamics of Active Elastic Microbeams.” <i>Physical Review X</i>. American Physical Society, 2025. <a href=\"https://doi.org/10.1103/rjk2-q2wh\">https://doi.org/10.1103/rjk2-q2wh</a>.","mla":"Martinet, Quentin, et al. “Emergent Dynamics of Active Elastic Microbeams.” <i>Physical Review X</i>, vol. 15, no. 4, 041017, American Physical Society, 2025, doi:<a href=\"https://doi.org/10.1103/rjk2-q2wh\">10.1103/rjk2-q2wh</a>.","ieee":"Q. Martinet, Y. I. Li, A. Aubret, E. B. Hannezo, and J. A. Palacci, “Emergent dynamics of active elastic microbeams,” <i>Physical Review X</i>, vol. 15, no. 4. American Physical Society, 2025.","short":"Q. Martinet, Y.I. Li, A. Aubret, E.B. Hannezo, J.A. Palacci, Physical Review X 15 (2025).","ama":"Martinet Q, Li YI, Aubret A, Hannezo EB, Palacci JA. Emergent dynamics of active elastic microbeams. <i>Physical Review X</i>. 2025;15(4). doi:<a href=\"https://doi.org/10.1103/rjk2-q2wh\">10.1103/rjk2-q2wh</a>","apa":"Martinet, Q., Li, Y. I., Aubret, A., Hannezo, E. B., &#38; Palacci, J. A. (2025). Emergent dynamics of active elastic microbeams. <i>Physical Review X</i>. American Physical Society. <a href=\"https://doi.org/10.1103/rjk2-q2wh\">https://doi.org/10.1103/rjk2-q2wh</a>"}},{"related_material":{"record":[{"status":"public","id":"20465","relation":"part_of_dissertation"}]},"page":"105","has_accepted_license":"1","project":[{"name":"Keratins in epithelial tissue spreading","grant_number":"PAT 5044023","_id":"8f060199-16d5-11f0-9cad-f3253b266c46"},{"_id":"25AA5F24-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Nano-Analytics of Cellular Systems","grant_number":"W 1250-B20"}],"type":"dissertation","article_processing_charge":"No","doi":"10.15479/AT-ISTA-20441","fulldoi":"https://doi.org/10.15479/AT-ISTA-20441","language":[{"iso":"eng"}],"oa_version":"Published Version","_id":"20441","day":"12","citation":{"chicago":"Naik, Suyash. “Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20441\">https://doi.org/10.15479/AT-ISTA-20441</a>.","ista":"Naik S. 2025. Keratins act as global coordinators of tissue spreading through mechanosensitive feedback. Institute of Science and Technology Austria.","short":"S. Naik, Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback, Institute of Science and Technology Austria, 2025.","ama":"Naik S. Keratins act as global coordinators of tissue spreading through mechanosensitive feedback. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20441\">10.15479/AT-ISTA-20441</a>","ieee":"S. Naik, “Keratins act as global coordinators of tissue spreading through mechanosensitive feedback,” Institute of Science and Technology Austria, 2025.","mla":"Naik, Suyash. <i>Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20441\">10.15479/AT-ISTA-20441</a>.","apa":"Naik, S. (2025). <i>Keratins act as global coordinators of tissue spreading through mechanosensitive feedback</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20441\">https://doi.org/10.15479/AT-ISTA-20441</a>"},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-069-5"]},"publication_status":"published","supervisor":[{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"},{"orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","last_name":"Hannezo"}],"title":"Keratins act as global coordinators of tissue spreading through mechanosensitive feedback","publisher":"Institute of Science and Technology Austria","alternative_title":["ISTA Thesis"],"abstract":[{"text":"Epithelial spreading plays a pivotal role in the development of organisms especially those\r\nsuch as zebrafish which require the epithelial enveloping layer (EVL) to spread to cover the\r\nsubstantial yolk surface during gastrulation. Epiboly requires the transition of the epithelium\r\nwith cuboidal cells to form a thin, flat squamous epithelial sheet. During this transition, the\r\ncells show tissue-scale mechanosensation with mechanisms such as direct mechanical control\r\nover the axis of cell division.\r\nCytoskeletal intermediate filaments play a crucial role in vertebrate cells, not only facilitating\r\nmechanical stability but also helping facilitate the mechanosensitive response of the cell.\r\nMechanosenstivity displayed by intermediate filaments is due not just to their interesting\r\nphysical properties but also to their interactions with other cytoskeletal elements such as actin\r\nand microtubules. Keratin is the predominant intermediate filament expressed in the EVL.\r\nIt expresses concomitantly with the gastrulation movements of the developing embryo. Our\r\nwork focuses on understanding the role and dynamics of the keratin cytoskeletal network in\r\nmodulating the physical aspects of EVL spreading. We demonstrated with the combination of\r\nphysical characterisation and manipulations of the EVL, utilising a variety of biophysical tools\r\nand microscopy, the mechanistic role of keratin in tissue spreading.\r\nGenerating novel genetic morphants and mutants, we probe the effect that the loss of the\r\nkeratin network has on the physiology of the epithelium and the developing embryo. We\r\nshow that the changing organisation of the keratin network is important for changing EVL\r\nphysical properties as the stress imposed on the EVL increases during epiboly. By modelling\r\nthe epithelium, we study how the mechanical heterogeneity in an epithelium can feed back into\r\na mechanical loop to the maturation of the keratin network and hence affect the mechanics\r\nof the epithelium. However, unlike what would be predicted by the effect of intermediate\r\nfilaments in acting as a security belt and increasing the resistance of the epithelium, we observe\r\nthat loss of keratin leads to a delay in the EVL movement. Using both local aspirations of the\r\nYSL and EVL ablations, we demonstrate the mechanistic facilitation of actin mechanosensation\r\nin a keratin-dependent manner.\r\nFurthermore, using chemical inhibitors of microtubule polymerisation, we provide insight into\r\nthe mechanisms underlying the organisation and distribution of keratin. Interestingly, the\r\nphenotype observed upon this loss of microtubules shows that keratins interact with the nucleus\r\nthrough microtubular interactions. Together with these diverse observations, we describe\r\nthe mechanosensory feedback between resilience and that is critical for uniform and robust\r\nspreading of the epithelium.","lang":"eng"}],"tmp":{"name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","short":"CC BY-SA (4.0)","image":"/images/cc_by_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode"},"corr_author":"1","author":[{"last_name":"Naik","full_name":"Naik, Suyash","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","first_name":"Suyash","orcid":"0000-0001-8421-5508"}],"file_date_updated":"2025-10-28T13:10:26Z","oa":1,"status":"public","ddc":["596","597","532"],"year":"2025","date_updated":"2026-07-06T12:51:41Z","file":[{"date_created":"2025-10-28T13:10:08Z","file_name":"Thesis_PDFA_.pdf","success":1,"checksum":"2892f04d4a5c18677871c3e06ac1244a","date_updated":"2025-10-28T13:10:08Z","access_level":"open_access","creator":"snaik","file_id":"20567","file_size":6846189,"relation":"main_file","content_type":"application/pdf"},{"file_id":"20568","file_size":8839300,"relation":"source_file","content_type":"application/zip","file_name":"Thesis.zip","date_created":"2025-10-28T13:10:26Z","checksum":"15934d4465cd0e9b7c32678da9a33a2f","date_updated":"2025-10-28T13:10:26Z","access_level":"open_access","creator":"snaik"}],"degree_awarded":"PhD","acknowledgement":"I would also like to thank the LSF and Cryo facility at ISTA, which have been helpful in my\r\nexperiments. I would also like to acknowledge FWF, grant DOI 10.55776/PAT5044023 and JKU Nanocell grant DOI \r\n10.55776/W1250 for providing funding for my PhD research. EMBO and FWF for providing funding for travel grants to attend conferences.","date_created":"2025-10-10T14:58:30Z","department":[{"_id":"GradSch"},{"_id":"CaHe"},{"_id":"EdHa"}],"date_published":"2025-10-12T00:00:00Z","OA_place":"publisher","month":"10","license":"https://creativecommons.org/licenses/by-sa/4.0/"},{"article_processing_charge":"No","status":"public","doi":"10.1101/2025.02.14.638262","fulldoi":"https://doi.org/10.1101/2025.02.14.638262","oa_version":"Preprint","publication":"bioRxiv","language":[{"iso":"eng"}],"year":"2025","_id":"20465","day":"17","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"20441"},{"relation":"later_version","status":"public","id":"22608"}]},"abstract":[{"text":"For tissues to spread, they must be deformable while maintaining their structural integrity. How these opposing requirements are balanced within spreading tissues is not yet well understood. Here, we show that keratin intermediate filaments function in epithelial spreading by adapting tissue mechanical resilience to the stresses arising in the tissue during the spreading process. By analysing the expansion of the enveloping cell layer (EVL) over the large yolk cell in early zebrafish embryos in vivo, we found that keratin network maturation in EVL cells is promoted by stresses building up within the spreading tissue. Through genetic interference and tissue rheology experiments, complemented by a vertex model with mechanochemical feedback, we demonstrate that stress-induced keratin network maturation in the EVL increases tissue viscosity, which is essential for preventing tissue rupture. Interestingly, keratins are also required in the yolk cell for mechanosensitive actomyosin network contraction and flow, the force-generating processes pulling the EVL. These dual mechanosensitive functions of keratins enable a balance between pulling force production in the yolk cell and the mechanical resilience of the EVL against stresses generated by these pulling forces, thereby ensuring uniform and robust tissue spreading.","lang":"eng"}],"tmp":{"name":"Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0)","short":"CC BY-ND (4.0)","image":"/image/cc_by_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nd/4.0/legalcode"},"corr_author":"1","author":[{"full_name":"Naik, Suyash","orcid":"0000-0001-8421-5508","first_name":"Suyash","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","last_name":"Naik"},{"first_name":"Yann-Edwin","full_name":"Keta, Yann-Edwin","last_name":"Keta"},{"last_name":"Pranjic-Ferscha","first_name":"Kornelija","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","full_name":"Pranjic-Ferscha, Kornelija"},{"last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"last_name":"Henkes","first_name":"Silke","full_name":"Henkes, Silke"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"type":"preprint","oa":1,"date_published":"2025-02-17T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_place":"repository","publication_status":"draft","title":"Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties","month":"02","license":"https://creativecommons.org/licenses/by-nd/4.0/","date_updated":"2026-07-29T10:33:31Z","das_tickbox":"1","citation":{"apa":"Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &#38; Heisenberg, C.-P. J. (n.d.). Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>","ama":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>","short":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J. Heisenberg, BioRxiv (n.d.).","ieee":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P. J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties,” <i>bioRxiv</i>. .","mla":"Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>.","chicago":"Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo, Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>.","ista":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. bioRxiv, <a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>."},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.02.14.638262"}],"date_created":"2025-10-14T07:25:27Z","department":[{"_id":"CaHe"},{"_id":"EdHa"}]},{"intvolume":"        17","pmid":1,"scopus_import":"1","quality_controlled":"1","OA_type":"closed access","status":"public","researchdata_availability":"no","volume":17,"year":"2025","publication":"Cold Spring Harbor Perspectives in Biology","publisher":"Cold Spring Harbor Laboratory Press","corr_author":"1","abstract":[{"text":"The importance of physical forces in the morphogenesis, homeostatic function, and pathological dysfunction of multicellular tissues is being increasingly characterized, both theoretically and experimentally. Analogies between biological systems and inert materials such as foams, gels, and liquid crystals have provided striking insights into the core design principles underlying multicellular organization. However, these connections can seem surprising given that a key feature of multicellular systems is their ability to constantly consume energy, providing an active origin for the forces that they produce. Key emerging questions are, therefore, to understand whether and how this activity grants tissues novel properties that do not have counterparts in classical materials, as well as their consequences for biological function. Here, we review recent discoveries at the intersection of active matter and tissue biology, with an emphasis on how modeling and experiments can be combined to understand the dynamics of multicellular systems. These approaches suggest that a number of key biological tissue-scale phenomena, such as morphogenetic shape changes, collective migration, or fate decisions, share unifying design principles that can be described by physical models of tissue active matter.","lang":"eng"}],"author":[{"last_name":"Brückner","orcid":"0000-0001-7205-2975","id":"e1e86031-6537-11eb-953a-f7ab92be508d","first_name":"David","full_name":"Brückner, David"},{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"}],"date_published":"2025-04-01T00:00:00Z","supplementarymaterial":"no","month":"04","das_tickbox":"0","date_updated":"2026-10-01T11:03:02Z","ec_funded":1,"acknowledgement":"We thank Fridtjof Brauns, Anna Kicheva, and Carl-Philipp Heisenberg for a critical reading of the manuscript and Claudia Flandoli for the artwork in the figures. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). This work received funding from the European Research Council (ERC) under the European Union\\u2019s Horizon 2020 Research and Innovation Programme Grant Agreement no. 851288.","date_created":"2025-01-29T13:33:47Z","department":[{"_id":"EdHa"}],"article_processing_charge":"No","doi":"10.1101/cshperspect.a041653","_id":"18960","day":"01","oa_version":"None","fulldoi":"https://doi.org/10.1101/cshperspect.a041653","language":[{"iso":"eng"}],"isi":1,"issue":"4","article_type":"original","type":"journal_article","project":[{"grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b"},{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"article_number":"a041653","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","external_id":{"pmid":["38951023"],"isi":["001456660400001"]},"publication_status":"published","publication_identifier":{"issn":["1943-0264"]},"title":"Tissue active matter: Integrating mechanics and signaling into dynamical models","citation":{"chicago":"Brückner, David, and Edouard B Hannezo. “Tissue Active Matter: Integrating Mechanics and Signaling into Dynamical Models.” <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press, 2025. <a href=\"https://doi.org/10.1101/cshperspect.a041653\">https://doi.org/10.1101/cshperspect.a041653</a>.","ista":"Brückner D, Hannezo EB. 2025. Tissue active matter: Integrating mechanics and signaling into dynamical models. Cold Spring Harbor Perspectives in Biology. 17(4), a041653.","apa":"Brückner, D., &#38; Hannezo, E. B. (2025). Tissue active matter: Integrating mechanics and signaling into dynamical models. <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press. <a href=\"https://doi.org/10.1101/cshperspect.a041653\">https://doi.org/10.1101/cshperspect.a041653</a>","short":"D. Brückner, E.B. Hannezo, Cold Spring Harbor Perspectives in Biology 17 (2025).","ama":"Brückner D, Hannezo EB. Tissue active matter: Integrating mechanics and signaling into dynamical models. <i>Cold Spring Harbor Perspectives in Biology</i>. 2025;17(4). doi:<a href=\"https://doi.org/10.1101/cshperspect.a041653\">10.1101/cshperspect.a041653</a>","ieee":"D. Brückner and E. B. Hannezo, “Tissue active matter: Integrating mechanics and signaling into dynamical models,” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 17, no. 4. Cold Spring Harbor Laboratory Press, 2025.","mla":"Brückner, David, and Edouard B. Hannezo. “Tissue Active Matter: Integrating Mechanics and Signaling into Dynamical Models.” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 17, no. 4, a041653, Cold Spring Harbor Laboratory Press, 2025, doi:<a href=\"https://doi.org/10.1101/cshperspect.a041653\">10.1101/cshperspect.a041653</a>."}},{"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"22804"}]},"project":[{"_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","call_identifier":"H2020","name":"IST-BRIDGE: International postdoctoral program","grant_number":"101034413"},{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"},{"_id":"9B861AAC-BA93-11EA-9121-9846C619BF3A","name":"NOMIS Fellowship Program"}],"type":"preprint","article_processing_charge":"No","fulldoi":"https://doi.org/10.1101/2025.10.15.682552","oa_version":"Preprint","language":[{"iso":"eng"}],"day":"15","_id":"22807","doi":"10.1101/2025.10.15.682552","main_file_link":[{"url":"https://doi.org/10.1101/2025.10.15.682552","open_access":"1"}],"citation":{"apa":"Tong, X., Li, Y. I., Schelle, J., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (n.d.). Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.10.15.682552\">https://doi.org/10.1101/2025.10.15.682552</a>","short":"X. Tong, Y.I. Li, J. Schelle, E.B. Hannezo, C.-P.J. Heisenberg, BioRxiv (n.d.).","ama":"Tong X, Li YI, Schelle J, Hannezo EB, Heisenberg C-PJ. Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.10.15.682552\">10.1101/2025.10.15.682552</a>","mla":"Tong, Xin, et al. “Non-Canonical Cytokinesis Driven by Mechanical Uncoupling via Nematic Flows and Adhesion-Based Invagination.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.10.15.682552\">10.1101/2025.10.15.682552</a>.","ieee":"X. Tong, Y. I. Li, J. Schelle, E. B. Hannezo, and C.-P. J. Heisenberg, “Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination,” <i>bioRxiv</i>. .","chicago":"Tong, Xin, Yuting I Li, Joséphine Schelle, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Non-Canonical Cytokinesis Driven by Mechanical Uncoupling via Nematic Flows and Adhesion-Based Invagination.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.10.15.682552\">https://doi.org/10.1101/2025.10.15.682552</a>.","ista":"Tong X, Li YI, Schelle J, Hannezo EB, Heisenberg C-PJ. Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination. bioRxiv, <a href=\"https://doi.org/10.1101/2025.10.15.682552\">10.1101/2025.10.15.682552</a>."},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","title":"Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination","publication_status":"draft","external_id":{"biorxivid":["2025.10.15.682552"]},"author":[{"id":"50F65CDC-AA30-11E9-A72B-8A12E6697425","first_name":"Xin","full_name":"Tong, Xin","last_name":"Tong"},{"id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","first_name":"Yuting I","full_name":"Li, Yuting I","last_name":"Li"},{"full_name":"Schelle, Joséphine","first_name":"Joséphine","last_name":"Schelle"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"oa":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png"},"abstract":[{"lang":"eng","text":"Cleavage - the series of rapid cell divisions that follow fertilization - marks the onset of metazoan development and represents a deeply conserved evolutionary process. Across animals, two principal modes exist: complete (holoblastic) and incomplete (meroblastic) cleavage. While holoblastic cleavage resembles conventional cytokinesis both in vitro and in vivo, the mechanisms underlying meroblastic cleavage have remained poorly understood. Using zebrafish embryos as a model, we show that meroblastic cleavage proceeds through a distinct two-step mechanism. The process begins with the assembly and contraction of a large, arc-shaped actomyosin cable. However, this contractile event alone is insufficient to complete division. A second phase, driven by cadherin-mediated membrane adhesion, is required to invaginate the furrow ridge. Strikingly, this transition depends on mechanical uncoupling of the contractile cable from the surrounding cortex. We demonstrate that such uncoupling arises from an active nematic instability, which both enhances contractility along the cable and generates actin depletion zones that relieve lateral connections. Together, these findings reveal that meroblastic cleavage is governed not by a single actomyosin-based event but by a sequential interplay between cytoskeletal contraction and cadherin-dependent adhesion, highlighting a mechanism fundamentally distinct from canonical cytokinesis."}],"corr_author":"1","status":"public","OA_type":"green","publication":"bioRxiv","year":"2025","date_updated":"2026-10-05T08:56:09Z","biorxivid":1,"department":[{"_id":"EdHa"},{"_id":"CaHe"}],"date_created":"2026-09-04T12:32:21Z","acknowledgement":"We are grateful to the members of the Hannezo and Heisenberg groups for discussions and technical advice. We also thank the Imaging and Optics Facility and the Lab Support Facility at ISTA for their continuous support. Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skodowska-Curie Grant Agreement No. 101034413. The research was supported by funding to C.-P.H. from the NOMIS Foundation (Project ID 1.844) and to E.H. from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 851288).","ec_funded":1,"date_published":"2025-10-15T00:00:00Z","month":"10","license":"https://creativecommons.org/licenses/by-nc/4.0/","OA_place":"repository"},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"citation":{"chicago":"Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>.","ista":"Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R, Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. Cell Reports. 44(3), 115387.","apa":"Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A., … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>","short":"S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild, C.-P.J. Heisenberg, Cell Reports 44 (2025).","ama":"Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>","mla":"Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>, vol. 44, no. 3, 115387, Cell Press, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>.","ieee":"S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Cell Press, 2025."},"external_id":{"isi":["001443652700001"],"pmid":["40057955"]},"publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"publication_status":"published","title":"BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation","article_number":"115387","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","has_accepted_license":"1","project":[{"_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development"},{"_id":"269CD5C4-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 1159-2018","name":"Mechanosensation in cell migration: the role of friction forces in cell polarization and directed migration"}],"isi":1,"issue":"3","article_type":"original","doi":"10.1016/j.celrep.2025.115387","day":"25","_id":"19404","fulldoi":"https://doi.org/10.1016/j.celrep.2025.115387","oa_version":"Published Version","language":[{"iso":"eng"}],"article_processing_charge":"Yes","acknowledgement":"We are grateful to the colleagues who contributed to this work with discussions, technical advice, and feedback on the manuscript: Irene Steccari, David Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship (ALTF 1159-2018).","date_created":"2025-03-16T23:01:24Z","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"Bio"}],"date_updated":"2026-10-20T08:20:45Z","file":[{"file_name":"2025_CellReports_Tavano.pdf","success":1,"date_created":"2025-03-17T10:26:54Z","checksum":"57e05dd1598c807af0afdb32cec039d3","creator":"dernst","date_updated":"2025-03-17T10:26:54Z","access_level":"open_access","file_id":"19413","file_size":9067797,"content_type":"application/pdf","relation":"main_file"}],"OA_place":"publisher","month":"03","date_published":"2025-03-25T00:00:00Z","abstract":[{"text":"Cell migration is a fundamental process during embryonic development. Most studies in vivo have focused on the migration of cells using the extracellular matrix (ECM) as their substrate for migration. In contrast, much less is known about how cells migrate on other cells, as found in early embryos when the ECM has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the early zebrafish gastrula use the ectoderm as their substrate for migration. We show that the lateral ectoderm is permissive for the animal-pole-directed migration of LME cells, while the ectoderm at the animal pole halts it. These differences in permissiveness depend on the lateral ectoderm being more cohesive than the animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion as one critical factor in regulating LME migration during zebrafish gastrulation.","lang":"eng"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"corr_author":"1","oa":1,"file_date_updated":"2025-03-17T10:26:54Z","author":[{"last_name":"Tavano","id":"2F162F0C-F248-11E8-B48F-1D18A9856A87","first_name":"Ste","orcid":"0000-0001-9970-7804","full_name":"Tavano, Ste"},{"first_name":"David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","orcid":"0000-0001-7205-2975","full_name":"Brückner, David","last_name":"Brückner"},{"full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","first_name":"Saren","last_name":"Tasciyan"},{"last_name":"Tong","id":"50F65CDC-AA30-11E9-A72B-8A12E6697425","first_name":"Xin","full_name":"Tong, Xin"},{"full_name":"Kardos, Roland","first_name":"Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87","last_name":"Kardos"},{"full_name":"Schauer, Alexandra","orcid":"0000-0001-7659-9142","first_name":"Alexandra","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","last_name":"Schauer"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg"}],"publisher":"Cell Press","year":"2025","volume":44,"publication":"Cell Reports","intvolume":"        44","scopus_import":"1","DOAJ_listed":"1","pmid":1,"status":"public","OA_type":"gold","ddc":["570"],"quality_controlled":"1"},{"intvolume":"        60","pmid":1,"PlanS_conform":"1","scopus_import":"1","OA_type":"hybrid","status":"public","ddc":["570"],"quality_controlled":"1","volume":60,"year":"2025","publication":"Developmental Cell","publisher":"Cell Press","abstract":[{"lang":"eng","text":"An enlarged brain underlies the complex central nervous system of vertebrates. The dramatic expansion of the brain that diverges its shape from the spinal cord follows neural tube closure during embryonic development. Here, we show that this differential deformation is encoded by a pre-pattern of tissue material properties in chicken embryos. Using magnetic droplets and atomic force microscopy, we demonstrate that the dorsal hindbrain is more fluid than the dorsal spinal cord, resulting in a thinning versus a resisting response to increasing lumen pressure, respectively. The dorsal hindbrain exhibits reduced apical actin and a disorganized laminin matrix consistent with tissue fluidization. Blocking the activity of neural-crest-associated matrix metalloproteinases inhibits hindbrain expansion. Transplanting dorsal hindbrain cells to the spinal cord can locally create an expanded brain-like morphology in some cases. Our findings raise questions in vertebrate head evolution and suggest a general role of mechanical pre-patterning in sculpting epithelial tubes."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"oa":1,"file_date_updated":"2025-12-29T13:45:05Z","author":[{"last_name":"Mclaren","first_name":"Susannah B.P.","full_name":"Mclaren, Susannah B.P."},{"first_name":"Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","full_name":"Xue, Shi-lei","last_name":"Xue"},{"full_name":"Ding, Siyuan","first_name":"Siyuan","last_name":"Ding"},{"last_name":"Winkel","full_name":"Winkel, Alexander K.","first_name":"Alexander K."},{"full_name":"Baldwin, Oscar","first_name":"Oscar","last_name":"Baldwin"},{"first_name":"Shreya","full_name":"Dwarakacherla, Shreya","last_name":"Dwarakacherla"},{"first_name":"Kristian","full_name":"Franze, Kristian","last_name":"Franze"},{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"first_name":"Fengzhu","full_name":"Xiong, Fengzhu","last_name":"Xiong"}],"date_published":"2025-09-08T00:00:00Z","OA_place":"publisher","month":"09","date_updated":"2026-10-20T08:25:00Z","file":[{"creator":"dernst","date_updated":"2025-12-29T13:45:05Z","access_level":"open_access","date_created":"2025-12-29T13:45:05Z","success":1,"file_name":"2025_DevelopmentalCell_McLaren.pdf","checksum":"1ca6f0822c1cbd430686d5e2a4f96401","content_type":"application/pdf","relation":"main_file","file_id":"20872","file_size":12564806}],"ec_funded":1,"date_created":"2025-05-18T22:02:50Z","acknowledgement":"We thank A. Dimitracopoulos, K. Kawaguchi, J. Vidigueira, B. Baum, I. McLaren, D. St Johnston, and members of the Buckley, Scarpa, Steventon, Kawaguchi, and Xiong labs for technical assistance and constructive feedback. We thank Ryan Greenhalgh for methods developed to obtain fluidity values from AFM data. We thank Nicola Lawrence, Alex Sossick, and Sargon Gross-Thebing from the Gurdon Institute Imaging Facility for microscopy support. Funding: this work was supported by a Wellcome Trust/Royal Society Sir Henry Dale Fellowship (215439/Z/19/Z) and UKRI-EPSRC Frontier Research Grant (EP/X023761/1, originally selected as an ERC Starting Grant) to F.X.; an ERC Consolidator Grant (772426), ERC Synergy Grant 101118729 UNFOLD, and Alexander von Humboldt Professorship ( Alexander von Humboldt Foundation) to K.F.; and an ERC Starting Grant (851288) to E.H.","department":[{"_id":"EdHa"}],"article_processing_charge":"Yes (in subscription journal)","doi":"10.1016/j.devcel.2025.04.010","_id":"19703","day":"08","fulldoi":"https://doi.org/10.1016/j.devcel.2025.04.010","language":[{"iso":"eng"}],"oa_version":"Published Version","isi":1,"issue":"17","article_type":"original","page":"2237-2247.e4","type":"journal_article","project":[{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"has_accepted_license":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"pmid":["40347948"],"isi":["001570502100005"]},"publication_status":"published","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"title":"Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord","citation":{"apa":"Mclaren, S. B. P., Xue, S., Ding, S., Winkel, A. K., Baldwin, O., Dwarakacherla, S., … Xiong, F. (2025). Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">https://doi.org/10.1016/j.devcel.2025.04.010</a>","ama":"Mclaren SBP, Xue S, Ding S, et al. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. <i>Developmental Cell</i>. 2025;60(17):2237-2247.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">10.1016/j.devcel.2025.04.010</a>","short":"S.B.P. Mclaren, S. Xue, S. Ding, A.K. Winkel, O. Baldwin, S. Dwarakacherla, K. Franze, E.B. Hannezo, F. Xiong, Developmental Cell 60 (2025) 2237–2247.e4.","mla":"Mclaren, Susannah B. P., et al. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” <i>Developmental Cell</i>, vol. 60, no. 17, Cell Press, 2025, p. 2237–2247.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">10.1016/j.devcel.2025.04.010</a>.","ieee":"S. B. P. Mclaren <i>et al.</i>, “Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord,” <i>Developmental Cell</i>, vol. 60, no. 17. Cell Press, p. 2237–2247.e4, 2025.","chicago":"Mclaren, Susannah B.P., Shi-lei Xue, Siyuan Ding, Alexander K. Winkel, Oscar Baldwin, Shreya Dwarakacherla, Kristian Franze, Edouard B Hannezo, and Fengzhu Xiong. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” <i>Developmental Cell</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">https://doi.org/10.1016/j.devcel.2025.04.010</a>.","ista":"Mclaren SBP, Xue S, Ding S, Winkel AK, Baldwin O, Dwarakacherla S, Franze K, Hannezo EB, Xiong F. 2025. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. Developmental Cell. 60(17), 2237–2247.e4."}},{"date_published":"2025-09-25T00:00:00Z","OA_place":"repository","month":"09","date_updated":"2026-10-21T22:31:29Z","das_tickbox":"1","date_created":"2026-03-11T08:40:06Z","acknowledgement":"European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian Academy of Sciences, 26360","department":[{"_id":"GradSch"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"AnSa"}],"status":"public","ddc":["539","570"],"publication":"bioRxiv","year":"2025","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"abstract":[{"text":"While tumor malignancy has been extensively studied under the prism of genetic and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal interactions with the microenvironment. This raises the hitherto untested possibility that heterogeneity of the untransformed tumor stroma can actively fuel malignant progression. As biological heterogeneity is inherently difficult to control, we adopted a reductionist approach and let tumor cells invade micro-engineered environments harboring obstacles with precision-controlled geometry. We find that not only the presence of obstacles, but more surprisingly their spatial disorder, causes a drastic shift from a collective to a single-cell mode of invasion – comparable in strength to cadherin loss. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell detachments result both from local geometrical constraints and a global integration of spatial disorder over time. We show that different types of microenvironments map onto different universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang (KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with quenched disorder (KPZq). Our findings highlight generic physical principles for how the mode of cancer cell invasion depends on environmental heterogeneity, with potential implications to understand tumor evolution in vivo.","lang":"eng"}],"corr_author":"1","author":[{"last_name":"Dunajova","full_name":"Dunajova, Zuzana","first_name":"Zuzana","id":"4B39F286-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Tasciyan","first_name":"Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren"},{"full_name":"Majek, Juraj","first_name":"Juraj","id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1","last_name":"Majek"},{"full_name":"Merrin, Jack","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","last_name":"Merrin"},{"first_name":"Erik","full_name":"Sahai, Erik","last_name":"Sahai"},{"last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"}],"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"draft","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening","citation":{"apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K., &#38; Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>","ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening,” <i>bioRxiv</i>. .","mla":"Dunajova, Zuzana, et al. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B. Hannezo, BioRxiv (n.d.).","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. bioRxiv, <a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>."},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.05.20.655037"}],"article_processing_charge":"No","doi":"10.1101/2025.05.20.655037","language":[{"iso":"eng"}],"fulldoi":"https://doi.org/10.1101/2025.05.20.655037","oa_version":"Preprint","day":"25","_id":"21427","related_material":{"record":[{"id":"21439","relation":"research_data","status":"public"},{"relation":"later_version","status":"public","id":"22963"},{"relation":"dissertation_contains","id":"21423","status":"public"}]},"has_accepted_license":"1","project":[{"grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"},{"name":"Motile active matter models of migrating cells and chiral filaments","grant_number":"26360","_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d"}],"type":"preprint"},{"doi_confirm":"1","date_published":"2025-09-24T00:00:00Z","month":"09","OA_place":"publisher","file":[{"file_size":41847994,"file_id":"20413","relation":"source_file","content_type":"application/x-zip-compressed","checksum":"6bb5a7ce318dc3f7bd165f2523e77b89","date_created":"2025-09-30T14:33:17Z","embargo_to":"open_access","file_name":"2025-Kishi-Kasumi-Thesis.zip","creator":"kkishi","access_level":"closed","date_updated":"2026-09-30T22:30:04Z"},{"checksum":"88349b9177e1dcbe1242cd3884b36fdb","date_created":"2025-09-30T14:33:22Z","file_name":"2025-Kishi-Kasumi-Thesis.pdf","creator":"kkishi","access_level":"open_access","date_updated":"2026-09-30T22:30:04Z","file_size":55747072,"file_id":"20414","content_type":"application/pdf","relation":"main_file","embargo":"2026-09-30"}],"date_updated":"2026-10-16T08:31:16Z","department":[{"_id":"GradSch"},{"_id":"AnKi"},{"_id":"EdHa"}],"date_created":"2025-09-25T10:08:10Z","degree_awarded":"PhD","ddc":["570"],"status":"public","year":"2025","alternative_title":["ISTA Thesis"],"publisher":"Institute of Science and Technology Austria","file_date_updated":"2026-09-30T22:30:04Z","oa":1,"author":[{"full_name":"Kishi, Kasumi","orcid":"0000-0001-6060-4795","id":"3065DFC4-F248-11E8-B48F-1D18A9856A87","first_name":"Kasumi","last_name":"Kishi"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"abstract":[{"lang":"eng","text":"During vertebrate development, the morphogen Sonic Hedgehog (Shh) plays a key role in \r\npatterning the tissues of the extending body. This requires precise coordination between the \r\nsize of the source of Shh production and the embryo body. Shh is produced by the notochord, \r\nas well as floorplate of the neural tube. In this thesis, we investigated how the sizes of the \r\nnotochord and floorplate are regulated and how they impact the developing mouse embryo.  \r\nTo measure the size and shape of the developing mouse notochord and the cell behaviours \r\nwithin this organ, we performed high-resolution measurements in cleared embryos between \r\nE8.5 and E10.5 of development. Strikingly, our data revealed that the notochord maintains a \r\nconstant cross-sectional area throughout the trunk over time as it lengthens by cell \r\nproliferation. We show that this is achieved by a feedback between the notochord cell \r\nproliferation rate and both the cross-sectional area of the trunk notochord and elongation of \r\nthe embryo. Using live imaging, we further show that notochord cells undergo posteriorly\r\ndirected active cell migration and that this cell migration contributes to maintaining the \r\nstructural integrity of the notochord. Our results show a complex coordination of cell \r\nbehaviours that allow for robust notochord morphogenesis during mouse development and \r\nthe maintenance of a defined Shh source size.  \r\nShh produced by the notochord induces a secondary Shh-producing domain, the floor plate. \r\nHow the notochord and floor plate contribute to the formation of the Shh gradient in the \r\nneural tube and to the regulation of the floor plate size is poorly understood. Our theoretical \r\nand experimental analysis indicates that only the initial levels of notochord-derived Shh \r\ninfluence the size of the floor plate, and the floor plate is insensitive to its own production of \r\nShh. After its initial establishment, the floor plate expands by tissue growth, leading to scaling \r\nof the Shh gradient amplitude with tissue size.   \r\nAltogether, we show that the mechanisms regulating the sizes of the notochord and \r\nfloorplate in mouse development help to achieve size robustness of these tissues. Our data \r\ndemonstrate that the initial establishment of the Shh gradient in the neural tube is strongly \r\ndependent on the notochord and suggest that the robustness of notochord morphogenesis \r\nis essential for pattern formation. Later in development, the floor plate size and the Shh \r\ngradient are coupled to neural tube growth. Our findings provide new insights on how pattern \r\nformation, tissue growth and morphogenesis interact to ensure reproducible developmental \r\noutcomes. "}],"corr_author":"1","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","title":"Regulation of notochord and floor plate size during mouse development","publication_identifier":{"issn":["2663-337X"]},"publication_status":"published","supervisor":[{"last_name":"Kicheva","orcid":"0000-0003-4509-4998","first_name":"Anna","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","full_name":"Kicheva, Anna"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B"}],"citation":{"ista":"Kishi K. 2025. Regulation of notochord and floor plate size during mouse development. Institute of Science and Technology Austria.","chicago":"Kishi, Kasumi. “Regulation of Notochord and Floor Plate Size during Mouse Development.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20393\">https://doi.org/10.15479/AT-ISTA-20393</a>.","apa":"Kishi, K. (2025). <i>Regulation of notochord and floor plate size during mouse development</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20393\">https://doi.org/10.15479/AT-ISTA-20393</a>","ieee":"K. Kishi, “Regulation of notochord and floor plate size during mouse development,” Institute of Science and Technology Austria, 2025.","mla":"Kishi, Kasumi. <i>Regulation of Notochord and Floor Plate Size during Mouse Development</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20393\">10.15479/AT-ISTA-20393</a>.","ama":"Kishi K. Regulation of notochord and floor plate size during mouse development. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20393\">10.15479/AT-ISTA-20393</a>","short":"K. Kishi, Regulation of Notochord and Floor Plate Size during Mouse Development, Institute of Science and Technology Austria, 2025."},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"}],"article_processing_charge":"No","day":"24","_id":"20393","oa_version":"Published Version","fulldoi":"https://doi.org/10.15479/AT-ISTA-20393","language":[{"iso":"eng"}],"doi":"10.15479/AT-ISTA-20393","page":"102","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"18481"}]},"type":"dissertation","has_accepted_license":"1"}]