[{"page":"267-274","type":"journal_article","isi":1,"ec_funded":1,"oa":1,"publication_status":"published","article_processing_charge":"No","year":"2021","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2021-02-01T00:00:00Z","abstract":[{"lang":"eng","text":"Collective cell migration offers a rich field of study for non-equilibrium physics and cellular biology, revealing phenomena such as glassy dynamics, pattern formation and active turbulence. However, how mechanical and chemical signalling are integrated at the cellular level to give rise to such collective behaviours remains unclear. We address this by focusing on the highly conserved phenomenon of spatiotemporal waves of density and extracellular signal-regulated kinase (ERK) activation, which appear both in vitro and in vivo during collective cell migration and wound healing. First, we propose a biophysical theory, backed by mechanical and optogenetic perturbation experiments, showing that patterns can be quantitatively explained by a mechanochemical coupling between active cellular tensions and the mechanosensitive ERK pathway. Next, we demonstrate how this biophysical mechanism can robustly induce long-ranged order and migration in a desired orientation, and we determine the theoretically optimal wavelength and period for inducing maximal migration towards free edges, which fits well with experimentally observed dynamics. We thereby provide a bridge between the biophysical origin of spatiotemporal instabilities and the design principles of robust and efficient long-ranged migration."}],"date_created":"2020-10-04T22:01:37Z","publisher":"Springer Nature","language":[{"iso":"eng"}],"intvolume":"        17","month":"02","corr_author":"1","status":"public","publication":"Nature Physics","day":"01","acknowledgement":"We would like to thank G. Tkacik and all of the members of the Hannezo and Hirashima groups for useful discussions, X. Trepat for help on traction force microscopy and M. Matsuda for use of the lab facility. E.H. acknowledges grants from the Austrian Science Fund (FWF) (P 31639) and the European Research Council (851288). T.H. acknowledges a grant from JST, PRESTO (JPMJPR1949). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 665385 (to D.B.), from JSPS KAKENHI grant no. 17J02107 (to N.H.) and from the SPIRITS 2018 of Kyoto University (to E.H. and T.H.).","article_type":"original","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"related_material":{"record":[{"status":"public","id":"12964","relation":"dissertation_contains"}],"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/wound-healing-waves/","relation":"press_release"}]},"external_id":{"isi":["000573519500002"]},"author":[{"first_name":"Daniel R","last_name":"Boocock","orcid":"0000-0002-1585-2631","full_name":"Boocock, Daniel R","id":"453AF628-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Hino, Naoya","first_name":"Naoya","last_name":"Hino"},{"id":"D2761128-D73D-11E9-A1BF-BA0DE6697425","full_name":"Ruzickova, Natalia","first_name":"Natalia","last_name":"Ruzickova"},{"full_name":"Hirashima, Tsuyoshi","last_name":"Hirashima","first_name":"Tsuyoshi"},{"first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"}],"title":"Theory of mechanochemical patterning and optimal migration in cell monolayers","date_updated":"2026-09-02T22:30:04Z","department":[{"_id":"EdHa"}],"scopus_import":"1","volume":17,"project":[{"name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639","call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425"},{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E"},{"name":"International IST Doctoral Program","grant_number":"665385","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"}],"doi":"10.1038/s41567-020-01037-7","citation":{"chicago":"Boocock, Daniel R, Naoya Hino, Natalia Ruzickova, Tsuyoshi Hirashima, and Edouard B Hannezo. “Theory of Mechanochemical Patterning and Optimal Migration in Cell Monolayers.” <i>Nature Physics</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41567-020-01037-7\">https://doi.org/10.1038/s41567-020-01037-7</a>.","short":"D.R. Boocock, N. Hino, N. Ruzickova, T. Hirashima, E.B. Hannezo, Nature Physics 17 (2021) 267–274.","ieee":"D. R. Boocock, N. Hino, N. Ruzickova, T. Hirashima, and E. B. Hannezo, “Theory of mechanochemical patterning and optimal migration in cell monolayers,” <i>Nature Physics</i>, vol. 17. Springer Nature, pp. 267–274, 2021.","mla":"Boocock, Daniel R., et al. “Theory of Mechanochemical Patterning and Optimal Migration in Cell Monolayers.” <i>Nature Physics</i>, vol. 17, Springer Nature, 2021, pp. 267–74, doi:<a href=\"https://doi.org/10.1038/s41567-020-01037-7\">10.1038/s41567-020-01037-7</a>.","apa":"Boocock, D. R., Hino, N., Ruzickova, N., Hirashima, T., &#38; Hannezo, E. B. (2021). Theory of mechanochemical patterning and optimal migration in cell monolayers. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-020-01037-7\">https://doi.org/10.1038/s41567-020-01037-7</a>","ama":"Boocock DR, Hino N, Ruzickova N, Hirashima T, Hannezo EB. Theory of mechanochemical patterning and optimal migration in cell monolayers. <i>Nature Physics</i>. 2021;17:267-274. doi:<a href=\"https://doi.org/10.1038/s41567-020-01037-7\">10.1038/s41567-020-01037-7</a>","ista":"Boocock DR, Hino N, Ruzickova N, Hirashima T, Hannezo EB. 2021. Theory of mechanochemical patterning and optimal migration in cell monolayers. Nature Physics. 17, 267–274."},"_id":"8602","oa_version":"Preprint","quality_controlled":"1","main_file_link":[{"url":"https://doi.org/10.1101/2020.05.15.096479","open_access":"1"}]},{"date_created":"2021-04-25T22:01:29Z","pmid":1,"abstract":[{"lang":"eng","text":"The way in which interactions between mechanics and biochemistry lead to the emergence of complex cell and tissue organization is an old question that has recently attracted renewed interest from biologists, physicists, mathematicians and computer scientists. Rapid advances in optical physics, microscopy and computational image analysis have greatly enhanced our ability to observe and quantify spatiotemporal patterns of signalling, force generation, deformation, and flow in living cells and tissues. Powerful new tools for genetic, biophysical and optogenetic manipulation are allowing us to perturb the underlying machinery that generates these patterns in increasingly sophisticated ways. Rapid advances in theory and computing have made it possible to construct predictive models that describe how cell and tissue organization and dynamics emerge from the local coupling of biochemistry and mechanics. Together, these advances have opened up a wealth of new opportunities to explore how mechanochemical patterning shapes organismal development. In this roadmap, we present a series of forward-looking case studies on mechanochemical patterning in development, written by scientists working at the interface between the physical and biological sciences, and covering a wide range of spatial and temporal scales, organisms, and modes of development. Together, these contributions highlight the many ways in which the dynamic coupling of mechanics and biochemistry shapes biological dynamics: from mechanoenzymes that sense force to tune their activity and motor output, to collectives of cells in tissues that flow and redistribute biochemical signals during development."}],"has_accepted_license":"1","publisher":"IOP Publishing","language":[{"iso":"eng"}],"intvolume":"        18","issue":"4","status":"public","month":"04","ddc":["570"],"publication":"Physical biology","type":"journal_article","isi":1,"ec_funded":1,"oa":1,"year":"2021","article_processing_charge":"No","publication_status":"published","file":[{"date_updated":"2021-04-27T08:38:35Z","success":1,"file_id":"9355","access_level":"open_access","content_type":"application/pdf","file_size":6296324,"creator":"cziletti","file_name":"2021_PhysBio_Lenne.pdf","checksum":"4f52082549d3561c4c15d4d8d84ca5d8","date_created":"2021-04-27T08:38:35Z","relation":"main_file"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_number":"041501","date_published":"2021-04-14T00:00:00Z","department":[{"_id":"AnKi"},{"_id":"EdHa"}],"scopus_import":"1","volume":18,"project":[{"call_identifier":"H2020","_id":"B6FC0238-B512-11E9-945C-1524E6697425","name":"Coordination of Patterning And Growth In the Spinal Cord","grant_number":"680037"},{"_id":"268294B6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"P31639","name":"Active mechano-chemical description of the cell cytoskeleton"},{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"doi":"10.1088/1478-3975/abd0db","citation":{"ieee":"P. F. Lenne <i>et al.</i>, “Roadmap for the multiscale coupling of biochemical and mechanical signals during development,” <i>Physical biology</i>, vol. 18, no. 4. IOP Publishing, 2021.","mla":"Lenne, Pierre François, et al. “Roadmap for the Multiscale Coupling of Biochemical and Mechanical Signals during Development.” <i>Physical Biology</i>, vol. 18, no. 4, 041501, IOP Publishing, 2021, doi:<a href=\"https://doi.org/10.1088/1478-3975/abd0db\">10.1088/1478-3975/abd0db</a>.","apa":"Lenne, P. F., Munro, E., Heemskerk, I., Warmflash, A., Bocanegra, L., Kishi, K., … Tlili, S. (2021). Roadmap for the multiscale coupling of biochemical and mechanical signals during development. <i>Physical Biology</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/1478-3975/abd0db\">https://doi.org/10.1088/1478-3975/abd0db</a>","ista":"Lenne PF, Munro E, Heemskerk I, Warmflash A, Bocanegra L, Kishi K, Kicheva A, Long Y, Fruleux A, Boudaoud A, Saunders TE, Caldarelli P, Michaut A, Gros J, Maroudas-Sacks Y, Keren K, Hannezo EB, Gartner ZJ, Stormo B, Gladfelter A, Rodrigues A, Shyer A, Minc N, Maître JL, Di Talia S, Khamaisi B, Sprinzak D, Tlili S. 2021. Roadmap for the multiscale coupling of biochemical and mechanical signals during development. Physical biology. 18(4), 041501.","ama":"Lenne PF, Munro E, Heemskerk I, et al. Roadmap for the multiscale coupling of biochemical and mechanical signals during development. <i>Physical biology</i>. 2021;18(4). doi:<a href=\"https://doi.org/10.1088/1478-3975/abd0db\">10.1088/1478-3975/abd0db</a>","chicago":"Lenne, Pierre François, Edwin Munro, Idse Heemskerk, Aryeh Warmflash, Laura Bocanegra, Kasumi Kishi, Anna Kicheva, et al. “Roadmap for the Multiscale Coupling of Biochemical and Mechanical Signals during Development.” <i>Physical Biology</i>. IOP Publishing, 2021. <a href=\"https://doi.org/10.1088/1478-3975/abd0db\">https://doi.org/10.1088/1478-3975/abd0db</a>.","short":"P.F. Lenne, E. Munro, I. Heemskerk, A. Warmflash, L. Bocanegra, K. Kishi, A. Kicheva, Y. Long, A. Fruleux, A. Boudaoud, T.E. Saunders, P. Caldarelli, A. Michaut, J. Gros, Y. Maroudas-Sacks, K. Keren, E.B. Hannezo, Z.J. Gartner, B. Stormo, A. Gladfelter, A. Rodrigues, A. Shyer, N. Minc, J.L. Maître, S. Di Talia, B. Khamaisi, D. Sprinzak, S. Tlili, Physical Biology 18 (2021)."},"_id":"9349","oa_version":"Published Version","quality_controlled":"1","article_type":"original","publication_identifier":{"eissn":["1478-3975"]},"related_material":{"record":[{"status":"public","id":"13081","relation":"dissertation_contains"}]},"file_date_updated":"2021-04-27T08:38:35Z","acknowledgement":"The AK group is supported by IST Austria and by the ERC under European Union Horizon 2020 research and innovation programme Grant 680037. Apologies to those whose work could not be mentioned due to limited space. We thank all my lab members, both past and present, for stimulating discussion. This work was funded by a Singapore Ministry of Education Tier 3 Grant, MOE2016-T3-1-005. We thank Francis Corson for continuous discussion and collaboration contributing to these views and for figure 4(A). PC is sponsored by the Institut Pasteur and the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Grant Agreement No. 665807. Research in JG's laboratory is funded by the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement No. 337635, Institut Pasteur, CNRS, Cercle FSER, Fondation pour la Recherche Medicale, the Vallee Foundation and the ANR-19-CE-13-0024 Grant. We thank Erez Braun and Alex Mogilner for comments on the manuscript and Niv Ierushalmi for help with figure 5. This project has received funding from the European Union's Horizon 2020 research and innovation programme under Grant Agreement No. ERC-2018-COG Grant 819174-HydraMechanics awarded to KK. EH thanks all lab members, as well as Pierre Recho, Tsuyoshi Hirashima, Diana Pinheiro and Carl-Philip Heisenberg, for fruitful discussions on these topics—and apologize for not being able to cite many very relevant publications due to the strict 10-reference limit. EH acknowledges the support of Austrian Science Fund (FWF) (P 31639) and the European Research Council under the European Union's Horizon 2020 Research and Innovation Programme Grant Agreements (851288). The authors acknowledge the inspiring scientists whose work could not be cited in this perspective due to space constraints; the members of the Gartner Lab for helpful discussions; the Barbara and Gerson Bakar Foundation, the Chan Zuckerberg Biohub Investigators Programme, the National Institute of Health, and the Centre for Cellular Construction, an NSF Science and Technology Centre. The Minc laboratory is currently funded by the CNRS and the European Research Council (CoG Forcaster No. 647073). Research in the lab of J-LM is supported by the Institut Curie, the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé Et de la Recherche Médicale (INSERM), and is funded by grants from the ATIP-Avenir programme, the Fondation Schlumberger pour l'Éducation et la Recherche via the Fondation pour la Recherche Médicale, the European Research Council Starting Grant ERC-2017-StG 757557, the European Molecular Biology Organization Young Investigator programme (EMBO YIP), the INSERM transversal programme Human Development Cell Atlas (HuDeCA), Paris Sciences Lettres (PSL) 'nouvelle équipe' and QLife (17-CONV-0005) grants and Labex DEEP (ANR-11-LABX-0044) which are part of the IDEX PSL (ANR-10-IDEX-0001-02). We acknowledge useful discussions with Massimo Vergassola, Sebastian Streichan and my lab members. Work in my laboratory on Drosophila embryogenesis is partly supported by NIH-R01GM122936. The authors acknowledge the support by a grant from the European Research Council (Grant No. 682161). Lenne group is funded by a grant from the 'Investissements d'Avenir' French Government programme managed by the French National Research Agency (ANR-16-CONV-0001) and by the Excellence Initiative of Aix-Marseille University—A*MIDEX, and ANR projects MechaResp (ANR-17-CE13-0032) and AdGastrulo (ANR-19-CE13-0022).","day":"14","external_id":{"isi":["000640396400001"],"pmid":["33276350"]},"author":[{"full_name":"Lenne, Pierre François","first_name":"Pierre François","last_name":"Lenne"},{"first_name":"Edwin","last_name":"Munro","full_name":"Munro, Edwin"},{"last_name":"Heemskerk","first_name":"Idse","full_name":"Heemskerk, Idse"},{"full_name":"Warmflash, Aryeh","last_name":"Warmflash","first_name":"Aryeh"},{"first_name":"Laura","last_name":"Bocanegra","id":"4896F754-F248-11E8-B48F-1D18A9856A87","full_name":"Bocanegra, Laura"},{"last_name":"Kishi","orcid":"0000-0001-6060-4795","first_name":"Kasumi","full_name":"Kishi, Kasumi","id":"3065DFC4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Anna","last_name":"Kicheva","orcid":"0000-0003-4509-4998","full_name":"Kicheva, Anna","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Yuchen","last_name":"Long","full_name":"Long, Yuchen"},{"full_name":"Fruleux, Antoine","first_name":"Antoine","last_name":"Fruleux"},{"full_name":"Boudaoud, Arezki","last_name":"Boudaoud","first_name":"Arezki"},{"full_name":"Saunders, Timothy E.","first_name":"Timothy E.","last_name":"Saunders"},{"last_name":"Caldarelli","first_name":"Paolo","full_name":"Caldarelli, Paolo"},{"first_name":"Arthur","last_name":"Michaut","full_name":"Michaut, Arthur"},{"full_name":"Gros, Jerome","first_name":"Jerome","last_name":"Gros"},{"full_name":"Maroudas-Sacks, Yonit","first_name":"Yonit","last_name":"Maroudas-Sacks"},{"full_name":"Keren, Kinneret","first_name":"Kinneret","last_name":"Keren"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"},{"full_name":"Gartner, Zev J.","first_name":"Zev J.","last_name":"Gartner"},{"full_name":"Stormo, Benjamin","first_name":"Benjamin","last_name":"Stormo"},{"full_name":"Gladfelter, Amy","first_name":"Amy","last_name":"Gladfelter"},{"full_name":"Rodrigues, Alan","first_name":"Alan","last_name":"Rodrigues"},{"first_name":"Amy","last_name":"Shyer","full_name":"Shyer, Amy"},{"first_name":"Nicolas","last_name":"Minc","full_name":"Minc, Nicolas"},{"full_name":"Maître, Jean Léon","first_name":"Jean Léon","last_name":"Maître"},{"first_name":"Stefano","last_name":"Di Talia","full_name":"Di Talia, Stefano"},{"full_name":"Khamaisi, Bassma","first_name":"Bassma","last_name":"Khamaisi"},{"full_name":"Sprinzak, David","last_name":"Sprinzak","first_name":"David"},{"last_name":"Tlili","first_name":"Sham","full_name":"Tlili, Sham"}],"date_updated":"2026-09-02T22:30:15Z","title":"Roadmap for the multiscale coupling of biochemical and mechanical signals during development"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2020-04-30T00:00:00Z","publication_status":"published","article_processing_charge":"No","year":"2020","file":[{"access_level":"open_access","content_type":"application/pdf","file_size":17992888,"creator":"dernst","checksum":"e2114902f4e9d75a752e9efb5ae06011","file_name":"2020_Cell_Dekoninck.pdf","date_created":"2020-05-04T10:20:55Z","relation":"main_file","date_updated":"2020-07-14T12:48:03Z","file_id":"7795"}],"isi":1,"type":"journal_article","oa":1,"page":"604-620.e22","month":"04","publication":"Cell","status":"public","ddc":["570"],"issue":"3","language":[{"iso":"eng"}],"intvolume":"       181","abstract":[{"lang":"eng","text":"During embryonic and postnatal development, organs and tissues grow steadily to achieve their final size at the end of puberty. However, little is known about the cellular dynamics that mediate postnatal growth. By combining in vivo clonal lineage tracing, proliferation kinetics, single-cell transcriptomics, andin vitro micro-pattern experiments, we resolved the cellular dynamics taking place during postnatal skin epidermis expansion. Our data revealed that harmonious growth is engineered by a single population of developmental progenitors presenting a fixed fate imbalance of self-renewing divisions with an ever-decreasing proliferation rate. Single-cell RNA sequencing revealed that epidermal developmental progenitors form a more uniform population compared with adult stem and progenitor cells. Finally, we found that the spatial pattern of cell division orientation is dictated locally by the underlying collagen fiber orientation. Our results uncover a simple design principle of organ growth where progenitors and differentiated cells expand in harmony with their surrounding tissues."}],"pmid":1,"date_created":"2020-05-03T22:00:48Z","has_accepted_license":"1","publisher":"Elsevier","title":"Defining the design principles of skin epidermis postnatal growth","date_updated":"2025-07-10T11:54:47Z","author":[{"full_name":"Dekoninck, Sophie","first_name":"Sophie","last_name":"Dekoninck"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"},{"full_name":"Sifrim, Alejandro","last_name":"Sifrim","first_name":"Alejandro"},{"last_name":"Miroshnikova","first_name":"Yekaterina A.","full_name":"Miroshnikova, Yekaterina A."},{"last_name":"Aragona","first_name":"Mariaceleste","full_name":"Aragona, Mariaceleste"},{"full_name":"Malfait, Milan","last_name":"Malfait","first_name":"Milan"},{"last_name":"Gargouri","first_name":"Souhir","full_name":"Gargouri, Souhir"},{"full_name":"De Neunheuser, Charlotte","first_name":"Charlotte","last_name":"De Neunheuser"},{"last_name":"Dubois","first_name":"Christine","full_name":"Dubois, Christine"},{"full_name":"Voet, Thierry","first_name":"Thierry","last_name":"Voet"},{"full_name":"Wickström, Sara A.","first_name":"Sara A.","last_name":"Wickström"},{"full_name":"Simons, Benjamin D.","first_name":"Benjamin D.","last_name":"Simons"},{"first_name":"Cédric","last_name":"Blanpain","full_name":"Blanpain, Cédric"}],"external_id":{"pmid":["32259486"],"isi":["000530708400016"]},"day":"30","file_date_updated":"2020-07-14T12:48:03Z","publication_identifier":{"issn":["0092-8674"],"eissn":["1097-4172"]},"article_type":"original","_id":"7789","oa_version":"Published Version","quality_controlled":"1","tmp":{"image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"citation":{"chicago":"Dekoninck, Sophie, Edouard B Hannezo, Alejandro Sifrim, Yekaterina A. Miroshnikova, Mariaceleste Aragona, Milan Malfait, Souhir Gargouri, et al. “Defining the Design Principles of Skin Epidermis Postnatal Growth.” <i>Cell</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">https://doi.org/10.1016/j.cell.2020.03.015</a>.","short":"S. Dekoninck, E.B. Hannezo, A. Sifrim, Y.A. Miroshnikova, M. Aragona, M. Malfait, S. Gargouri, C. De Neunheuser, C. Dubois, T. Voet, S.A. Wickström, B.D. Simons, C. Blanpain, Cell 181 (2020) 604–620.e22.","mla":"Dekoninck, Sophie, et al. “Defining the Design Principles of Skin Epidermis Postnatal Growth.” <i>Cell</i>, vol. 181, no. 3, Elsevier, 2020, p. 604–620.e22, doi:<a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">10.1016/j.cell.2020.03.015</a>.","ieee":"S. Dekoninck <i>et al.</i>, “Defining the design principles of skin epidermis postnatal growth,” <i>Cell</i>, vol. 181, no. 3. Elsevier, p. 604–620.e22, 2020.","ama":"Dekoninck S, Hannezo EB, Sifrim A, et al. Defining the design principles of skin epidermis postnatal growth. <i>Cell</i>. 2020;181(3):604-620.e22. doi:<a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">10.1016/j.cell.2020.03.015</a>","ista":"Dekoninck S, Hannezo EB, Sifrim A, Miroshnikova YA, Aragona M, Malfait M, Gargouri S, De Neunheuser C, Dubois C, Voet T, Wickström SA, Simons BD, Blanpain C. 2020. Defining the design principles of skin epidermis postnatal growth. Cell. 181(3), 604–620.e22.","apa":"Dekoninck, S., Hannezo, E. B., Sifrim, A., Miroshnikova, Y. A., Aragona, M., Malfait, M., … Blanpain, C. (2020). Defining the design principles of skin epidermis postnatal growth. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">https://doi.org/10.1016/j.cell.2020.03.015</a>"},"doi":"10.1016/j.cell.2020.03.015","scopus_import":"1","department":[{"_id":"EdHa"}],"volume":181},{"corr_author":"1","month":"07","status":"public","ddc":["570"],"publication":"Proceedings of the National Academy of Sciences of the United States of America","issue":"29","intvolume":"       117","language":[{"iso":"eng"}],"publisher":"National Academy of Sciences","has_accepted_license":"1","date_created":"2020-08-09T22:00:52Z","pmid":1,"abstract":[{"lang":"eng","text":"Understanding to what extent stem cell potential is a cell-intrinsic property or an emergent behavior coming from global tissue dynamics and geometry is a key outstanding question of systems and stem cell biology. Here, we propose a theory of stem cell dynamics as a stochastic competition for access to a spatially localized niche, giving rise to a stochastic conveyor-belt model. Cell divisions produce a steady cellular stream which advects cells away from the niche, while random rearrangements enable cells away from the niche to be favorably repositioned. Importantly, even when assuming that all cells in a tissue are molecularly equivalent, we predict a common (“universal”) functional dependence of the long-term clonal survival probability on distance from the niche, as well as the emergence of a well-defined number of functional stem cells, dependent only on the rate of random movements vs. mitosis-driven advection. We test the predictions of this theory on datasets of pubertal mammary gland tips and embryonic kidney tips, as well as homeostatic intestinal crypts. Importantly, we find good agreement for the predicted functional dependency of the competition as a function of position, and thus functional stem cell number in each organ. This argues for a key role of positional fluctuations in dictating stem cell number and dynamics, and we discuss the applicability of this theory to other settings."}],"date_published":"2020-07-21T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","file":[{"file_name":"2020_PNAS_Corominas.pdf","creator":"dernst","relation":"main_file","date_created":"2020-08-10T06:50:28Z","content_type":"application/pdf","access_level":"open_access","file_size":1111604,"file_id":"8223","success":1,"date_updated":"2020-08-10T06:50:28Z"}],"publication_status":"published","article_processing_charge":"No","year":"2020","oa":1,"type":"journal_article","ec_funded":1,"isi":1,"page":"16969-16975","quality_controlled":"1","oa_version":"Published Version","_id":"8220","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"citation":{"short":"B. Corominas-Murtra, C.L.G.J. Scheele, K. Kishi, S.I.J. Ellenbroek, B.D. Simons, J. Van Rheenen, E.B. Hannezo, Proceedings of the National Academy of Sciences of the United States of America 117 (2020) 16969–16975.","chicago":"Corominas-Murtra, Bernat, Colinda L.G.J. Scheele, Kasumi Kishi, Saskia I.J. Ellenbroek, Benjamin D. Simons, Jacco Van Rheenen, and Edouard B Hannezo. “Stem Cell Lineage Survival as a Noisy Competition for Niche Access.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2020. <a href=\"https://doi.org/10.1073/pnas.1921205117\">https://doi.org/10.1073/pnas.1921205117</a>.","ista":"Corominas-Murtra B, Scheele CLGJ, Kishi K, Ellenbroek SIJ, Simons BD, Van Rheenen J, Hannezo EB. 2020. Stem cell lineage survival as a noisy competition for niche access. Proceedings of the National Academy of Sciences of the United States of America. 117(29), 16969–16975.","apa":"Corominas-Murtra, B., Scheele, C. L. G. J., Kishi, K., Ellenbroek, S. I. J., Simons, B. D., Van Rheenen, J., &#38; Hannezo, E. B. (2020). Stem cell lineage survival as a noisy competition for niche access. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1921205117\">https://doi.org/10.1073/pnas.1921205117</a>","ama":"Corominas-Murtra B, Scheele CLGJ, Kishi K, et al. Stem cell lineage survival as a noisy competition for niche access. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2020;117(29):16969-16975. doi:<a href=\"https://doi.org/10.1073/pnas.1921205117\">10.1073/pnas.1921205117</a>","ieee":"B. Corominas-Murtra <i>et al.</i>, “Stem cell lineage survival as a noisy competition for niche access,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 117, no. 29. National Academy of Sciences, pp. 16969–16975, 2020.","mla":"Corominas-Murtra, Bernat, et al. “Stem Cell Lineage Survival as a Noisy Competition for Niche Access.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 117, no. 29, National Academy of Sciences, 2020, pp. 16969–75, doi:<a href=\"https://doi.org/10.1073/pnas.1921205117\">10.1073/pnas.1921205117</a>."},"doi":"10.1073/pnas.1921205117","project":[{"grant_number":"851288","name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"}],"volume":117,"scopus_import":"1","department":[{"_id":"EdHa"}],"title":"Stem cell lineage survival as a noisy competition for niche access","date_updated":"2026-04-03T09:29:04Z","author":[{"id":"43BE2298-F248-11E8-B48F-1D18A9856A87","full_name":"Corominas-Murtra, Bernat","first_name":"Bernat","orcid":"0000-0001-9806-5643","last_name":"Corominas-Murtra"},{"full_name":"Scheele, Colinda L.G.J.","first_name":"Colinda L.G.J.","last_name":"Scheele"},{"first_name":"Kasumi","last_name":"Kishi","orcid":"0000-0001-6060-4795","full_name":"Kishi, Kasumi","id":"3065DFC4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ellenbroek, Saskia I.J.","last_name":"Ellenbroek","first_name":"Saskia I.J."},{"full_name":"Simons, Benjamin D.","last_name":"Simons","first_name":"Benjamin D."},{"full_name":"Van Rheenen, Jacco","last_name":"Van Rheenen","first_name":"Jacco"},{"orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"}],"external_id":{"pmid":["32611816"],"isi":["000553292900014"]},"day":"21","file_date_updated":"2020-08-10T06:50:28Z","acknowledgement":"We thank all members of the E.H., B.D.S., and J.v.R. groups for stimulating discussions. This project was supported by\r\nthe European Research Council (648804 to J.v.R. and 851288 to E.H.). It has also received support from the CancerGenomics.nl (Netherlands Organization for Scientific Research) program (J.v.R.) and the Doctor Josef Steiner Foundation (J.v.R). B.D.S. was supported by Royal Society E. P. Abraham Research Professorship RP/R1/180165 and Wellcome Trust Grant 098357/Z/12/Z.","article_type":"original","related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/order-from-noise/"}]},"publication_identifier":{"eissn":["1091-6490"]}},{"oa":1,"type":"journal_article","isi":1,"file":[{"file_size":5540540,"content_type":"application/pdf","access_level":"open_access","relation":"main_file","date_created":"2020-10-19T11:27:46Z","checksum":"0ecc0eab72d2d50694852579611a6624","file_name":"2020_NatureComm_Sznurkowska.pdf","creator":"dernst","date_updated":"2020-10-19T11:27:46Z","success":1,"file_id":"8677"}],"article_processing_charge":"No","year":"2020","publication_status":"published","article_number":"5037","date_published":"2020-10-07T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publisher":"Springer Nature","has_accepted_license":"1","pmid":1,"date_created":"2020-10-18T22:01:35Z","abstract":[{"text":"Pancreatic islets play an essential role in regulating blood glucose level. Although the molecular pathways underlying islet cell differentiation are beginning to be resolved, the cellular basis of islet morphogenesis and fate allocation remain unclear. By combining unbiased and targeted lineage tracing, we address the events leading to islet formation in the mouse. From the statistical analysis of clones induced at multiple embryonic timepoints, here we show that, during the secondary transition, islet formation involves the aggregation of multiple equipotent endocrine progenitors that transition from a phase of stochastic amplification by cell division into a phase of sublineage restriction and limited islet fission. Together, these results explain quantitatively the heterogeneous size distribution and degree of polyclonality of maturing islets, as well as dispersion of progenitors within and between islets. Further, our results show that, during the secondary transition, α- and β-cells are generated in a contemporary manner. Together, these findings provide insight into the cellular basis of islet development.","lang":"eng"}],"intvolume":"        11","language":[{"iso":"eng"}],"ddc":["570"],"month":"10","status":"public","publication":"Nature Communications","publication_identifier":{"eissn":["2041-1723"]},"file_date_updated":"2020-10-19T11:27:46Z","article_type":"original","day":"07","external_id":{"pmid":["33028844"],"isi":["000577244600003"]},"author":[{"full_name":"Sznurkowska, Magdalena K.","first_name":"Magdalena K.","last_name":"Sznurkowska"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"},{"full_name":"Azzarelli, Roberta","last_name":"Azzarelli","first_name":"Roberta"},{"first_name":"Lemonia","last_name":"Chatzeli","full_name":"Chatzeli, Lemonia"},{"last_name":"Ikeda","first_name":"Tatsuro","full_name":"Ikeda, Tatsuro"},{"last_name":"Yoshida","first_name":"Shosei","full_name":"Yoshida, Shosei"},{"full_name":"Philpott, Anna","first_name":"Anna","last_name":"Philpott"},{"first_name":"Benjamin D","last_name":"Simons","full_name":"Simons, Benjamin D"}],"date_updated":"2026-04-02T14:29:58Z","title":"Tracing the cellular basis of islet specification in mouse pancreas","volume":11,"scopus_import":"1","department":[{"_id":"EdHa"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"citation":{"short":"M.K. Sznurkowska, E.B. Hannezo, R. Azzarelli, L. Chatzeli, T. Ikeda, S. Yoshida, A. Philpott, B.D. Simons, Nature Communications 11 (2020).","chicago":"Sznurkowska, Magdalena K., Edouard B Hannezo, Roberta Azzarelli, Lemonia Chatzeli, Tatsuro Ikeda, Shosei Yoshida, Anna Philpott, and Benjamin D Simons. “Tracing the Cellular Basis of Islet Specification in Mouse Pancreas.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-18837-3\">https://doi.org/10.1038/s41467-020-18837-3</a>.","apa":"Sznurkowska, M. K., Hannezo, E. B., Azzarelli, R., Chatzeli, L., Ikeda, T., Yoshida, S., … Simons, B. D. (2020). Tracing the cellular basis of islet specification in mouse pancreas. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-18837-3\">https://doi.org/10.1038/s41467-020-18837-3</a>","ista":"Sznurkowska MK, Hannezo EB, Azzarelli R, Chatzeli L, Ikeda T, Yoshida S, Philpott A, Simons BD. 2020. Tracing the cellular basis of islet specification in mouse pancreas. Nature Communications. 11, 5037.","ama":"Sznurkowska MK, Hannezo EB, Azzarelli R, et al. Tracing the cellular basis of islet specification in mouse pancreas. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-18837-3\">10.1038/s41467-020-18837-3</a>","ieee":"M. K. Sznurkowska <i>et al.</i>, “Tracing the cellular basis of islet specification in mouse pancreas,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","mla":"Sznurkowska, Magdalena K., et al. “Tracing the Cellular Basis of Islet Specification in Mouse Pancreas.” <i>Nature Communications</i>, vol. 11, 5037, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-18837-3\">10.1038/s41467-020-18837-3</a>."},"doi":"10.1038/s41467-020-18837-3","oa_version":"Published Version","quality_controlled":"1","_id":"8669"},{"file":[{"relation":"main_file","date_created":"2021-02-04T10:20:02Z","file_name":"2020_DevelopmCell_Chaigne.pdf","creator":"dernst","checksum":"88e1a031a61689165d19a19c2f16d795","file_size":6929686,"content_type":"application/pdf","access_level":"open_access","success":1,"file_id":"9086","date_updated":"2021-02-04T10:20:02Z"}],"article_processing_charge":"No","year":"2020","publication_status":"published","date_published":"2020-10-26T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"195-208","oa":1,"type":"journal_article","isi":1,"issue":"2","publication":"Developmental Cell","ddc":["570"],"status":"public","month":"10","publisher":"Elsevier","has_accepted_license":"1","abstract":[{"lang":"eng","text":"Cell fate transitions are key to development and homeostasis. It is thus essential to understand the cellular mechanisms controlling fate transitions. Cell division has been implicated in fate decisions in many stem cell types, including neuronal and epithelial progenitors. In other stem cells, such as embryonic stem (ES) cells, the role of division remains unclear. Here, we show that exit from naive pluripotency in mouse ES cells generally occurs after a division. We further show that exit timing is strongly correlated between sister cells, which remain connected by cytoplasmic bridges long after division, and that bridge abscission progressively accelerates as cells exit naive pluripotency. Finally, interfering with abscission impairs naive pluripotency exit, and artificially inducing abscission accelerates it. Altogether, our data indicate that a switch in the division machinery leading to faster abscission regulates pluripotency exit. Our study identifies abscission as a key cellular process coupling cell division to fate transitions."}],"date_created":"2020-10-18T22:01:37Z","pmid":1,"intvolume":"        55","language":[{"iso":"eng"}],"author":[{"full_name":"Chaigne, Agathe","last_name":"Chaigne","first_name":"Agathe"},{"full_name":"Labouesse, Céline","first_name":"Céline","last_name":"Labouesse"},{"full_name":"White, Ian J.","first_name":"Ian J.","last_name":"White"},{"first_name":"Meghan","last_name":"Agnew","full_name":"Agnew, Meghan"},{"first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"},{"full_name":"Chalut, Kevin J.","first_name":"Kevin J.","last_name":"Chalut"},{"full_name":"Paluch, Ewa K.","last_name":"Paluch","first_name":"Ewa K."}],"date_updated":"2025-07-10T11:57:15Z","title":"Abscission couples cell division to embryonic stem cell fate","file_date_updated":"2021-02-04T10:20:02Z","acknowledgement":"This work was supported by the Medical Research Council UK (MRC Program award MC_UU_12018/5 ), the European Research Council (starting grant 311637 -MorphoCorDiv and consolidator grant 820188 -NanoMechShape to E.K.P.), and the Leverhulme Trust (Leverhulme Prize in Biological Sciences to E.K.P.). K.J.C. acknowledges support from the Royal Society (Royal Society Research Fellowship). A.C. acknowledges support from EMBO ( ALTF 2015-563 ), the Wellcome Trust ( 201334/Z/16/Z ), and the Fondation Bettencourt-Schueller (Prix Jeune Chercheur, 2015).","article_type":"original","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"day":"26","external_id":{"isi":["000582501100012"],"pmid":["32979313"]},"quality_controlled":"1","oa_version":"Published Version","_id":"8672","volume":55,"department":[{"_id":"EdHa"}],"scopus_import":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"doi":"10.1016/j.devcel.2020.09.001","citation":{"chicago":"Chaigne, Agathe, Céline Labouesse, Ian J. White, Meghan Agnew, Edouard B Hannezo, Kevin J. Chalut, and Ewa K. Paluch. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” <i>Developmental Cell</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">https://doi.org/10.1016/j.devcel.2020.09.001</a>.","short":"A. Chaigne, C. Labouesse, I.J. White, M. Agnew, E.B. Hannezo, K.J. Chalut, E.K. Paluch, Developmental Cell 55 (2020) 195–208.","ieee":"A. Chaigne <i>et al.</i>, “Abscission couples cell division to embryonic stem cell fate,” <i>Developmental Cell</i>, vol. 55, no. 2. Elsevier, pp. 195–208, 2020.","mla":"Chaigne, Agathe, et al. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” <i>Developmental Cell</i>, vol. 55, no. 2, Elsevier, 2020, pp. 195–208, doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">10.1016/j.devcel.2020.09.001</a>.","apa":"Chaigne, A., Labouesse, C., White, I. J., Agnew, M., Hannezo, E. B., Chalut, K. J., &#38; Paluch, E. K. (2020). Abscission couples cell division to embryonic stem cell fate. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">https://doi.org/10.1016/j.devcel.2020.09.001</a>","ama":"Chaigne A, Labouesse C, White IJ, et al. Abscission couples cell division to embryonic stem cell fate. <i>Developmental Cell</i>. 2020;55(2):195-208. doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">10.1016/j.devcel.2020.09.001</a>","ista":"Chaigne A, Labouesse C, White IJ, Agnew M, Hannezo EB, Chalut KJ, Paluch EK. 2020. Abscission couples cell division to embryonic stem cell fate. Developmental Cell. 55(2), 195–208."}},{"citation":{"chicago":"Ucar, Mehmet C, and Reinhard Lipowsky. “Collective Force Generation by Molecular Motors Is Determined by Strain-Induced Unbinding.” <i>Nano Letters</i>. American Chemical Society, 2020. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445\">https://doi.org/10.1021/acs.nanolett.9b04445</a>.","short":"M.C. Ucar, R. Lipowsky, Nano Letters 20 (2020) 669–676.","mla":"Ucar, Mehmet C., and Reinhard Lipowsky. “Collective Force Generation by Molecular Motors Is Determined by Strain-Induced Unbinding.” <i>Nano Letters</i>, vol. 20, no. 1, American Chemical Society, 2020, pp. 669–76, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445\">10.1021/acs.nanolett.9b04445</a>.","ieee":"M. C. Ucar and R. Lipowsky, “Collective force generation by molecular motors is determined by strain-induced unbinding,” <i>Nano Letters</i>, vol. 20, no. 1. American Chemical Society, pp. 669–676, 2020.","ama":"Ucar MC, Lipowsky R. Collective force generation by molecular motors is determined by strain-induced unbinding. <i>Nano Letters</i>. 2020;20(1):669-676. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445\">10.1021/acs.nanolett.9b04445</a>","ista":"Ucar MC, Lipowsky R. 2020. Collective force generation by molecular motors is determined by strain-induced unbinding. Nano Letters. 20(1), 669–676.","apa":"Ucar, M. C., &#38; Lipowsky, R. (2020). Collective force generation by molecular motors is determined by strain-induced unbinding. <i>Nano Letters</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445\">https://doi.org/10.1021/acs.nanolett.9b04445</a>"},"doi":"10.1021/acs.nanolett.9b04445","scopus_import":"1","department":[{"_id":"EdHa"}],"volume":20,"_id":"7166","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1021/acs.nanolett.9b04445"}],"oa_version":"Published Version","quality_controlled":"1","external_id":{"pmid":["31797672"],"isi":["000507151600087"]},"publication_identifier":{"issn":["1530-6984"],"eissn":["1530-6992"]},"article_type":"letter_note","related_material":{"record":[{"relation":"research_data","id":"9726","status":"public"},{"relation":"research_data","id":"9885","status":"public"}]},"day":"08","date_updated":"2026-07-06T12:14:33Z","title":"Collective force generation by molecular motors is determined by strain-induced unbinding","author":[{"id":"50B2A802-6007-11E9-A42B-EB23E6697425","full_name":"Ucar, Mehmet C","orcid":"0000-0003-0506-4217","last_name":"Ucar","first_name":"Mehmet C"},{"last_name":"Lipowsky","first_name":"Reinhard","full_name":"Lipowsky, Reinhard"}],"language":[{"iso":"eng"}],"intvolume":"        20","pmid":1,"abstract":[{"lang":"eng","text":"In the living cell, we encounter a large variety of motile processes such as organelle transport and cytoskeleton remodeling. These processes are driven by motor proteins that generate force by transducing chemical free energy into mechanical work. In many cases, the molecular motors work in teams to collectively generate larger forces. Recent optical trapping experiments on small teams of cytoskeletal motors indicated that the collectively generated force increases with the size of the motor team but that this increase depends on the motor type and on whether the motors are studied in vitro or in vivo. Here, we use the theory of stochastic processes to describe the motion of N motors in a stationary optical trap and to compute the N-dependence of the collectively generated forces. We consider six distinct motor types, two kinesins, two dyneins, and two myosins. We show that the force increases always linearly with N but with a prefactor that depends on the performance of the single motor. Surprisingly, this prefactor increases for weaker motors with a lower stall force. This counter-intuitive behavior reflects the increased probability with which stronger motors detach from the filament during strain generation. Our theoretical results are in quantitative agreement with experimental data on small teams of kinesin-1 motors."}],"date_created":"2019-12-10T15:36:05Z","publisher":"American Chemical Society","month":"01","ddc":["570"],"status":"public","publication":"Nano Letters","corr_author":"1","issue":"1","type":"journal_article","isi":1,"oa":1,"page":"669-676","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2020-01-08T00:00:00Z","article_processing_charge":"No","year":"2020","publication_status":"published"},{"author":[{"id":"50B2A802-6007-11E9-A42B-EB23E6697425","full_name":"Ucar, Mehmet C","first_name":"Mehmet C","orcid":"0000-0003-0506-4217","last_name":"Ucar"},{"full_name":"Lipowsky, Reinhard","first_name":"Reinhard","last_name":"Lipowsky"}],"year":"2020","article_processing_charge":"No","title":"MURL_Dataz","date_updated":"2026-07-06T12:14:33Z","date_published":"2020-01-08T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"08","related_material":{"record":[{"relation":"used_in_publication","id":"7166","status":"public"}]},"type":"research_data_reference","oa_version":"Published Version","month":"01","status":"public","_id":"9885","publisher":"American Chemical Society","date_created":"2021-08-11T13:16:03Z","abstract":[{"lang":"eng","text":"Data obtained from the fine-grained simulations used in Figures 2-5, data obtained from the coarse-grained numerical calculations used in Figure 6, and a sample script for the fine-grained simulation as a Jupyter notebook (ZIP)"}],"department":[{"_id":"EdHa"}],"doi":"10.1021/acs.nanolett.9b04445.s002","citation":{"mla":"Ucar, Mehmet C., and Reinhard Lipowsky. <i>MURL_Dataz</i>. American Chemical Society, 2020, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">10.1021/acs.nanolett.9b04445.s002</a>.","ieee":"M. C. Ucar and R. Lipowsky, “MURL_Dataz.” American Chemical Society, 2020.","apa":"Ucar, M. C., &#38; Lipowsky, R. (2020). MURL_Dataz. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">https://doi.org/10.1021/acs.nanolett.9b04445.s002</a>","ista":"Ucar MC, Lipowsky R. 2020. MURL_Dataz, American Chemical Society, <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">10.1021/acs.nanolett.9b04445.s002</a>.","ama":"Ucar MC, Lipowsky R. MURL_Dataz. 2020. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">10.1021/acs.nanolett.9b04445.s002</a>","chicago":"Ucar, Mehmet C, and Reinhard Lipowsky. “MURL_Dataz.” American Chemical Society, 2020. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">https://doi.org/10.1021/acs.nanolett.9b04445.s002</a>.","short":"M.C. Ucar, R. Lipowsky, (2020)."}},{"intvolume":"        17","language":[{"iso":"eng"}],"publisher":"Royal Society","abstract":[{"text":"In many real-world systems, information can be transmitted in two qualitatively different ways: by copying or by transformation. Copying occurs when messages are transmitted without modification, e.g. when an offspring receives an unaltered copy of a gene from its parent. Transformation occurs when messages are modified systematically during transmission, e.g. when mutational biases occur during genetic replication. Standard information-theoretic measures do not distinguish these two modes of information transfer, although they may reflect different mechanisms and have different functional consequences. Starting from a few simple axioms, we derive a decomposition of mutual information into the information transmitted by copying versus the information transmitted by transformation. We begin with a decomposition that applies when the source and destination of the channel have the same set of messages and a notion of message identity exists. We then generalize our decomposition to other kinds of channels, which can involve different source and destination sets and broader notions of similarity. In addition, we show that copy information can be interpreted as the minimal work needed by a physical copying process, which is relevant for understanding the physics of replication. We use the proposed decomposition to explore a model of amino acid substitution rates. Our results apply to any system in which the fidelity of copying, rather than simple predictability, is of critical relevance.","lang":"eng"}],"pmid":1,"date_created":"2020-02-02T23:01:03Z","status":"public","month":"01","publication":"Journal of the Royal Society Interface","issue":"162","oa":1,"type":"journal_article","isi":1,"article_number":"0623","date_published":"2020-01-29T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2020","article_processing_charge":"No","publication_status":"published","citation":{"short":"A. Kolchinsky, B. Corominas-Murtra, Journal of the Royal Society Interface 17 (2020).","chicago":"Kolchinsky, Artemy, and Bernat Corominas-Murtra. “Decomposing Information into Copying versus Transformation.” <i>Journal of the Royal Society Interface</i>. Royal Society, 2020. <a href=\"https://doi.org/10.1098/rsif.2019.0623\">https://doi.org/10.1098/rsif.2019.0623</a>.","apa":"Kolchinsky, A., &#38; Corominas-Murtra, B. (2020). Decomposing information into copying versus transformation. <i>Journal of the Royal Society Interface</i>. Royal Society. <a href=\"https://doi.org/10.1098/rsif.2019.0623\">https://doi.org/10.1098/rsif.2019.0623</a>","ista":"Kolchinsky A, Corominas-Murtra B. 2020. Decomposing information into copying versus transformation. Journal of the Royal Society Interface. 17(162), 0623.","ama":"Kolchinsky A, Corominas-Murtra B. Decomposing information into copying versus transformation. <i>Journal of the Royal Society Interface</i>. 2020;17(162). doi:<a href=\"https://doi.org/10.1098/rsif.2019.0623\">10.1098/rsif.2019.0623</a>","ieee":"A. Kolchinsky and B. Corominas-Murtra, “Decomposing information into copying versus transformation,” <i>Journal of the Royal Society Interface</i>, vol. 17, no. 162. Royal Society, 2020.","mla":"Kolchinsky, Artemy, and Bernat Corominas-Murtra. “Decomposing Information into Copying versus Transformation.” <i>Journal of the Royal Society Interface</i>, vol. 17, no. 162, 0623, Royal Society, 2020, doi:<a href=\"https://doi.org/10.1098/rsif.2019.0623\">10.1098/rsif.2019.0623</a>."},"doi":"10.1098/rsif.2019.0623","volume":17,"scopus_import":"1","department":[{"_id":"EdHa"}],"main_file_link":[{"url":"https://arxiv.org/abs/1903.10693","open_access":"1"}],"oa_version":"Preprint","quality_controlled":"1","_id":"7431","external_id":{"arxiv":["1903.10693"],"pmid":["31964273"],"isi":["000538369800002"]},"arxiv":1,"article_type":"original","publication_identifier":{"eissn":["1742-5662"]},"acknowledgement":"AK was supported by Grant No. FQXi-RFP-1622 from the FQXi foundation, and Grant No. CHE-1648973 from the U.S.\r\nNational Science Foundation. AK would like to thank the Santa Fe Institute for supporting this research. The authors\r\nthank Jordi Fortuny, Rudolf Hanel, Joshua Garland, and Blai Vidiella for helpful discussions, as well as the anonymous\r\nreviewers for their insightful suggestions. ","day":"29","date_updated":"2026-08-12T14:08:47Z","title":"Decomposing information into copying versus transformation","author":[{"full_name":"Kolchinsky, Artemy","last_name":"Kolchinsky","first_name":"Artemy"},{"last_name":"Corominas-Murtra","orcid":"0000-0001-9806-5643","first_name":"Bernat","full_name":"Corominas-Murtra, Bernat","id":"43BE2298-F248-11E8-B48F-1D18A9856A87"}]},{"external_id":{"isi":["000457468300011"],"pmid":["30559456"]},"day":"01","related_material":{"link":[{"url":"https://ist.ac.at/en/news/when-a-fish-becomes-fluid/","relation":"press_release","description":"News on IST Homepage"}]},"article_type":"original","file_date_updated":"2020-10-21T07:18:35Z","publication_identifier":{"issn":["1465-7392"]},"title":"Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling","date_updated":"2025-07-10T11:52:59Z","author":[{"first_name":"Nicoletta","orcid":"0000-0002-8451-1195","last_name":"Petridou","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","full_name":"Petridou, Nicoletta"},{"full_name":"Grigolon, Silvia","first_name":"Silvia","last_name":"Grigolon"},{"first_name":"Guillaume","last_name":"Salbreux","full_name":"Salbreux, Guillaume"},{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561","first_name":"Edouard B"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"_id":"253E54C8-B435-11E9-9278-68D0E5697425","grant_number":"ALTF710-2016","name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants"}],"citation":{"ista":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. 2019. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. 21, 169–178.","apa":"Petridou, N., Grigolon, S., Salbreux, G., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2019). Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41556-018-0247-4\">https://doi.org/10.1038/s41556-018-0247-4</a>","ama":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. <i>Nature Cell Biology</i>. 2019;21:169–178. doi:<a href=\"https://doi.org/10.1038/s41556-018-0247-4\">10.1038/s41556-018-0247-4</a>","ieee":"N. Petridou, S. Grigolon, G. Salbreux, E. B. Hannezo, and C.-P. J. Heisenberg, “Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling,” <i>Nature Cell Biology</i>, vol. 21. Nature Publishing Group, pp. 169–178, 2019.","mla":"Petridou, Nicoletta, et al. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” <i>Nature Cell Biology</i>, vol. 21, Nature Publishing Group, 2019, pp. 169–178, doi:<a href=\"https://doi.org/10.1038/s41556-018-0247-4\">10.1038/s41556-018-0247-4</a>.","short":"N. Petridou, S. Grigolon, G. Salbreux, E.B. Hannezo, C.-P.J. Heisenberg, Nature Cell Biology 21 (2019) 169–178.","chicago":"Petridou, Nicoletta, Silvia Grigolon, Guillaume Salbreux, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2019. <a href=\"https://doi.org/10.1038/s41556-018-0247-4\">https://doi.org/10.1038/s41556-018-0247-4</a>."},"doi":"10.1038/s41556-018-0247-4","scopus_import":"1","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"volume":21,"_id":"5789","oa_version":"Submitted Version","quality_controlled":"1","type":"journal_article","ec_funded":1,"isi":1,"oa":1,"page":"169–178","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2019-02-01T00:00:00Z","publication_status":"published","year":"2019","article_processing_charge":"No","file":[{"relation":"main_file","date_created":"2020-10-21T07:18:35Z","checksum":"e38523787b3bc84006f2793de99ad70f","creator":"dernst","file_name":"2018_NatureCellBio_Petridou_accepted.pdf","file_size":71590590,"content_type":"application/pdf","access_level":"open_access","success":1,"file_id":"8685","date_updated":"2020-10-21T07:18:35Z"}],"language":[{"iso":"eng"}],"intvolume":"        21","date_created":"2018-12-30T22:59:15Z","pmid":1,"has_accepted_license":"1","abstract":[{"text":"Tissue morphogenesis is driven by mechanical forces that elicit changes in cell size, shape and motion. The extent by which forces deform tissues critically depends on the rheological properties of the recipient tissue. Yet, whether and how dynamic changes in tissue rheology affect tissue morphogenesis and how they are regulated within the developing organism remain unclear. Here, we show that blastoderm spreading at the onset of zebrafish morphogenesis relies on a rapid, pronounced and spatially patterned tissue fluidization. Blastoderm fluidization is temporally controlled by mitotic cell rounding-dependent cell–cell contact disassembly during the last rounds of cell cleavages. Moreover, fluidization is spatially restricted to the central blastoderm by local activation of non-canonical Wnt signalling within the blastoderm margin, increasing cell cohesion and thereby counteracting the effect of mitotic rounding on contact disassembly. Overall, our results identify a fluidity transition mediated by loss of cell cohesion as a critical regulator of embryo morphogenesis.","lang":"eng"}],"publisher":"Nature Publishing Group","ddc":["570"],"publication":"Nature Cell Biology","month":"02","status":"public","acknowledged_ssus":[{"_id":"Bio"}]},{"isi":1,"type":"journal_article","oa":1,"publication_status":"published","article_processing_charge":"No","year":"2019","file":[{"creator":"dernst","file_name":"2019_Life_Corominas.pdf","checksum":"7d2322cd96ace41959909b66702d5cf4","date_created":"2019-02-11T10:45:27Z","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_size":963454,"file_id":"5951","date_updated":"2020-07-14T12:47:13Z"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_published":"2019-01-15T00:00:00Z","article_number":"9","abstract":[{"text":"Understanding the thermodynamics of the duplication process is a fundamental step towards a comprehensive physical theory of biological systems. However, the immense complexity of real cells obscures the fundamental tensions between energy gradients and entropic contributions that underlie duplication. The study of synthetic, feasible systems reproducing part of the key ingredients of living entities but overcoming major sources of biological complexity is of great relevance to deepen the comprehension of the fundamental thermodynamic processes underlying life and its prevalence. In this paper an abstract—yet realistic—synthetic system made of small synthetic protocell aggregates is studied in detail. A fundamental relation between free energy and entropic gradients is derived for a general, non-equilibrium scenario, setting the thermodynamic conditions for the occurrence and prevalence of duplication phenomena. This relation sets explicitly how the energy gradients invested in creating and maintaining structural—and eventually, functional—elements of the system must always compensate the entropic gradients, whose contributions come from changes in the translational, configurational, and macrostate entropies, as well as from dissipation due to irreversible transitions. Work/energy relations are also derived, defining lower bounds on the energy required for the duplication event to take place. A specific example including real ternary emulsions is provided in order to grasp the orders of magnitude involved in the problem. It is found that the minimal work invested over the system to trigger a duplication event is around ~ 10−13J , which results, in the case of duplication of all the vesicles contained in a liter of emulsion, in an amount of energy around ~ 1kJ . Without aiming to describe a truly biological process of duplication, this theoretical contribution seeks to explicitly define and identify the key actors that participate in it.","lang":"eng"}],"has_accepted_license":"1","date_created":"2019-02-10T22:59:15Z","publisher":"MDPI","language":[{"iso":"eng"}],"intvolume":"         9","issue":"1","status":"public","ddc":["570"],"month":"01","publication":"Life","day":"15","publication_identifier":{"eissn":["2075-1729"]},"file_date_updated":"2020-07-14T12:47:13Z","external_id":{"isi":["000464125500001"]},"author":[{"orcid":"0000-0001-9806-5643","last_name":"Corominas-Murtra","first_name":"Bernat","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","full_name":"Corominas-Murtra, Bernat"}],"title":"Thermodynamics of duplication thresholds in synthetic protocell systems","date_updated":"2026-04-03T09:37:52Z","scopus_import":"1","department":[{"_id":"EdHa"}],"volume":9,"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"doi":"10.3390/life9010009","citation":{"short":"B. Corominas-Murtra, Life 9 (2019).","chicago":"Corominas-Murtra, Bernat. “Thermodynamics of Duplication Thresholds in Synthetic Protocell Systems.” <i>Life</i>. MDPI, 2019. <a href=\"https://doi.org/10.3390/life9010009\">https://doi.org/10.3390/life9010009</a>.","ama":"Corominas-Murtra B. Thermodynamics of duplication thresholds in synthetic protocell systems. <i>Life</i>. 2019;9(1). doi:<a href=\"https://doi.org/10.3390/life9010009\">10.3390/life9010009</a>","ista":"Corominas-Murtra B. 2019. Thermodynamics of duplication thresholds in synthetic protocell systems. Life. 9(1), 9.","apa":"Corominas-Murtra, B. (2019). Thermodynamics of duplication thresholds in synthetic protocell systems. <i>Life</i>. MDPI. <a href=\"https://doi.org/10.3390/life9010009\">https://doi.org/10.3390/life9010009</a>","ieee":"B. Corominas-Murtra, “Thermodynamics of duplication thresholds in synthetic protocell systems,” <i>Life</i>, vol. 9, no. 1. MDPI, 2019.","mla":"Corominas-Murtra, Bernat. “Thermodynamics of Duplication Thresholds in Synthetic Protocell Systems.” <i>Life</i>, vol. 9, no. 1, 9, MDPI, 2019, doi:<a href=\"https://doi.org/10.3390/life9010009\">10.3390/life9010009</a>."},"_id":"5944","quality_controlled":"1","oa_version":"Published Version"},{"language":[{"iso":"eng"}],"intvolume":"       116","has_accepted_license":"1","date_created":"2019-03-31T21:59:13Z","pmid":1,"abstract":[{"text":"The formation of self-organized patterns is key to the morphogenesis of multicellular organisms, although a comprehensive theory of biological pattern formation is still lacking. Here, we propose a minimal model combining tissue mechanics with morphogen turnover and transport to explore routes to patterning. Our active description couples morphogen reaction and diffusion, which impact cell differentiation and tissue mechanics, to a two-phase poroelastic rheology, where one tissue phase consists of a poroelastic cell network and the other one of a permeating extracellular fluid, which provides a feedback by actively transporting morphogens. While this model encompasses previous theories approximating tissues to inert monophasic media, such as Turing’s reaction–diffusion model, it overcomes some of their key limitations permitting pattern formation via any two-species biochemical kinetics due to mechanically induced cross-diffusion flows. Moreover, we describe a qualitatively different advection-driven Keller–Segel instability which allows for the formation of patterns with a single morphogen and whose fundamental mode pattern robustly scales with tissue size. We discuss the potential relevance of these findings for tissue morphogenesis.","lang":"eng"}],"publisher":"National Academy of Sciences","corr_author":"1","publication":"Proceedings of the National Academy of Sciences of the United States of America","status":"public","month":"03","ddc":["570"],"issue":"12","type":"journal_article","isi":1,"oa":1,"page":"5344-5349","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2019-03-19T00:00:00Z","publication_status":"published","article_processing_charge":"No","year":"2019","file":[{"content_type":"application/pdf","access_level":"open_access","file_size":3456045,"checksum":"8b67eee0ea8e5db61583e4d485215258","creator":"dernst","file_name":"2019_PNAS_Recho.pdf","relation":"main_file","date_created":"2019-04-03T14:10:30Z","date_updated":"2020-07-14T12:47:23Z","file_id":"6193"}],"project":[{"call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425","name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"citation":{"short":"P. Recho, A. Hallou, E.B. Hannezo, Proceedings of the National Academy of Sciences of the United States of America 116 (2019) 5344–5349.","chicago":"Recho, Pierre, Adrien Hallou, and Edouard B Hannezo. “Theory of Mechanochemical Patterning in Biphasic Biological Tissues.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2019. <a href=\"https://doi.org/10.1073/pnas.1813255116\">https://doi.org/10.1073/pnas.1813255116</a>.","ista":"Recho P, Hallou A, Hannezo EB. 2019. Theory of mechanochemical patterning in biphasic biological tissues. Proceedings of the National Academy of Sciences of the United States of America. 116(12), 5344–5349.","ama":"Recho P, Hallou A, Hannezo EB. Theory of mechanochemical patterning in biphasic biological tissues. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2019;116(12):5344-5349. doi:<a href=\"https://doi.org/10.1073/pnas.1813255116\">10.1073/pnas.1813255116</a>","apa":"Recho, P., Hallou, A., &#38; Hannezo, E. B. (2019). Theory of mechanochemical patterning in biphasic biological tissues. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1813255116\">https://doi.org/10.1073/pnas.1813255116</a>","mla":"Recho, Pierre, et al. “Theory of Mechanochemical Patterning in Biphasic Biological Tissues.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 116, no. 12, National Academy of Sciences, 2019, pp. 5344–49, doi:<a href=\"https://doi.org/10.1073/pnas.1813255116\">10.1073/pnas.1813255116</a>.","ieee":"P. Recho, A. Hallou, and E. B. Hannezo, “Theory of mechanochemical patterning in biphasic biological tissues,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 116, no. 12. National Academy of Sciences, pp. 5344–5349, 2019."},"doi":"10.1073/pnas.1813255116","department":[{"_id":"EdHa"}],"scopus_import":"1","volume":116,"_id":"6191","oa_version":"Published Version","quality_controlled":"1","external_id":{"isi":["000461679000027"],"pmid":["30819884"]},"day":"19","file_date_updated":"2020-07-14T12:47:23Z","related_material":{"link":[{"url":"www.pnas.org/lookup/suppl/doi:10.1073/pnas.1813255116/-/DCSupplemental","relation":"supplementary_material"}]},"publication_identifier":{"issn":["0027-8424"],"eissn":["1091-6490"]},"title":"Theory of mechanochemical patterning in biphasic biological tissues","date_updated":"2025-07-10T11:53:14Z","author":[{"last_name":"Recho","first_name":"Pierre","full_name":"Recho, Pierre"},{"full_name":"Hallou, Adrien","first_name":"Adrien","last_name":"Hallou"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"}]},{"citation":{"mla":"Guiu, Jordi, et al. “Tracing the Origin of Adult Intestinal Stem Cells.” <i>Nature</i>, vol. 570, Springer Nature, 2019, pp. 107–11, doi:<a href=\"https://doi.org/10.1038/s41586-019-1212-5\">10.1038/s41586-019-1212-5</a>.","ieee":"J. Guiu <i>et al.</i>, “Tracing the origin of adult intestinal stem cells,” <i>Nature</i>, vol. 570. Springer Nature, pp. 107–111, 2019.","ama":"Guiu J, Hannezo EB, Yui S, et al. Tracing the origin of adult intestinal stem cells. <i>Nature</i>. 2019;570:107-111. doi:<a href=\"https://doi.org/10.1038/s41586-019-1212-5\">10.1038/s41586-019-1212-5</a>","ista":"Guiu J, Hannezo EB, Yui S, Demharter S, Ulyanchenko S, Maimets M, Jørgensen A, Perlman S, Lundvall L, Mamsen LS, Larsen A, Olesen RH, Andersen CY, Thuesen LL, Hare KJ, Pers TH, Khodosevich K, Simons BD, Jensen KB. 2019. Tracing the origin of adult intestinal stem cells. Nature. 570, 107–111.","apa":"Guiu, J., Hannezo, E. B., Yui, S., Demharter, S., Ulyanchenko, S., Maimets, M., … Jensen, K. B. (2019). Tracing the origin of adult intestinal stem cells. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-019-1212-5\">https://doi.org/10.1038/s41586-019-1212-5</a>","chicago":"Guiu, Jordi, Edouard B Hannezo, Shiro Yui, Samuel Demharter, Svetlana Ulyanchenko, Martti Maimets, Anne Jørgensen, et al. “Tracing the Origin of Adult Intestinal Stem Cells.” <i>Nature</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41586-019-1212-5\">https://doi.org/10.1038/s41586-019-1212-5</a>.","short":"J. Guiu, E.B. Hannezo, S. Yui, S. Demharter, S. Ulyanchenko, M. Maimets, A. Jørgensen, S. Perlman, L. Lundvall, L.S. Mamsen, A. Larsen, R.H. Olesen, C.Y. Andersen, L.L. Thuesen, K.J. Hare, T.H. Pers, K. Khodosevich, B.D. Simons, K.B. Jensen, Nature 570 (2019) 107–111."},"doi":"10.1038/s41586-019-1212-5","department":[{"_id":"EdHa"}],"scopus_import":"1","volume":570,"_id":"6513","oa_version":"Submitted Version","quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986928"}],"external_id":{"isi":["000470149000048"],"pmid":["31092921"]},"day":"06","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"article_type":"original","title":"Tracing the origin of adult intestinal stem cells","date_updated":"2025-07-10T11:53:29Z","author":[{"full_name":"Guiu, Jordi","first_name":"Jordi","last_name":"Guiu"},{"orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"},{"last_name":"Yui","first_name":"Shiro","full_name":"Yui, Shiro"},{"last_name":"Demharter","first_name":"Samuel","full_name":"Demharter, Samuel"},{"last_name":"Ulyanchenko","first_name":"Svetlana","full_name":"Ulyanchenko, Svetlana"},{"full_name":"Maimets, Martti","last_name":"Maimets","first_name":"Martti"},{"last_name":"Jørgensen","first_name":"Anne","full_name":"Jørgensen, Anne"},{"first_name":"Signe","last_name":"Perlman","full_name":"Perlman, Signe"},{"first_name":"Lene","last_name":"Lundvall","full_name":"Lundvall, Lene"},{"full_name":"Mamsen, Linn Salto","first_name":"Linn Salto","last_name":"Mamsen"},{"full_name":"Larsen, Agnete","last_name":"Larsen","first_name":"Agnete"},{"full_name":"Olesen, Rasmus H.","first_name":"Rasmus H.","last_name":"Olesen"},{"last_name":"Andersen","first_name":"Claus Yding","full_name":"Andersen, Claus Yding"},{"full_name":"Thuesen, Lea Langhoff","first_name":"Lea Langhoff","last_name":"Thuesen"},{"last_name":"Hare","first_name":"Kristine Juul","full_name":"Hare, Kristine Juul"},{"full_name":"Pers, Tune H.","last_name":"Pers","first_name":"Tune H."},{"last_name":"Khodosevich","first_name":"Konstantin","full_name":"Khodosevich, Konstantin"},{"full_name":"Simons, Benjamin D.","last_name":"Simons","first_name":"Benjamin D."},{"first_name":"Kim B.","last_name":"Jensen","full_name":"Jensen, Kim B."}],"language":[{"iso":"eng"}],"intvolume":"       570","abstract":[{"lang":"eng","text":"Adult intestinal stem cells are located at the bottom of crypts of Lieberkühn, where they express markers such as LGR5 1,2 and fuel the constant replenishment of the intestinal epithelium1. Although fetal LGR5-expressing cells can give rise to adult intestinal stem cells3,4, it remains unclear whether this population in the patterned epithelium represents unique intestinal stem-cell precursors. Here we show, using unbiased quantitative lineage-tracing approaches, biophysical modelling and intestinal transplantation, that all cells of the mouse intestinal epithelium—irrespective of their location and pattern of LGR5 expression in the fetal gut tube—contribute actively to the adult intestinal stem cell pool. Using 3D imaging, we find that during fetal development the villus undergoes gross remodelling and fission. This brings epithelial cells from the non-proliferative villus into the proliferative intervillus region, which enables them to contribute to the adult stem-cell niche. Our results demonstrate that large-scale remodelling of the intestinal wall and cell-fate specification are closely linked. Moreover, these findings provide a direct link between the observed plasticity and cellular reprogramming of differentiating cells in adult tissues following damage5,6,7,8,9, revealing that stem-cell identity is an induced rather than a hardwired property."}],"pmid":1,"date_created":"2019-06-02T21:59:14Z","publisher":"Springer Nature","status":"public","month":"06","publication":"Nature","type":"journal_article","isi":1,"oa":1,"page":"107-111","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2019-06-06T00:00:00Z","publication_status":"published","year":"2019","article_processing_charge":"No"},{"intvolume":"        60","language":[{"iso":"eng"}],"publisher":"Elsevier","date_created":"2019-06-16T21:59:12Z","abstract":[{"text":"Branching morphogenesis is a prototypical example of complex three-dimensional organ sculpting, required in multiple developmental settings to maximize the area of exchange surfaces. It requires, in particular, the coordinated growth of different cell types together with complex patterning to lead to robust macroscopic outputs. In recent years, novel multiscale quantitative biology approaches, together with biophysical modelling, have begun to shed new light of this topic. Here, we wish to review some of these recent developments, highlighting the generic design principles that can be abstracted across different branched organs, as well as the implications for the broader fields of stem cell, developmental and systems biology.","lang":"eng"}],"pmid":1,"status":"public","month":"10","publication":"Current Opinion in Cell Biology","type":"journal_article","isi":1,"page":"99-105","date_published":"2019-10-01T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_status":"published","article_processing_charge":"No","year":"2019","doi":"10.1016/j.ceb.2019.04.008","citation":{"short":"E.B. Hannezo, B.D. Simons, Current Opinion in Cell Biology 60 (2019) 99–105.","chicago":"Hannezo, Edouard B, and Benjamin D. Simons. “Multiscale Dynamics of Branching Morphogenesis.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.ceb.2019.04.008\">https://doi.org/10.1016/j.ceb.2019.04.008</a>.","ama":"Hannezo EB, Simons BD. Multiscale dynamics of branching morphogenesis. <i>Current Opinion in Cell Biology</i>. 2019;60:99-105. doi:<a href=\"https://doi.org/10.1016/j.ceb.2019.04.008\">10.1016/j.ceb.2019.04.008</a>","apa":"Hannezo, E. B., &#38; Simons, B. D. (2019). Multiscale dynamics of branching morphogenesis. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2019.04.008\">https://doi.org/10.1016/j.ceb.2019.04.008</a>","ista":"Hannezo EB, Simons BD. 2019. Multiscale dynamics of branching morphogenesis. Current Opinion in Cell Biology. 60, 99–105.","mla":"Hannezo, Edouard B., and Benjamin D. Simons. “Multiscale Dynamics of Branching Morphogenesis.” <i>Current Opinion in Cell Biology</i>, vol. 60, Elsevier, 2019, pp. 99–105, doi:<a href=\"https://doi.org/10.1016/j.ceb.2019.04.008\">10.1016/j.ceb.2019.04.008</a>.","ieee":"E. B. Hannezo and B. D. Simons, “Multiscale dynamics of branching morphogenesis,” <i>Current Opinion in Cell Biology</i>, vol. 60. Elsevier, pp. 99–105, 2019."},"volume":60,"department":[{"_id":"EdHa"}],"scopus_import":"1","oa_version":"None","quality_controlled":"1","_id":"6559","external_id":{"isi":["000486545800014"],"pmid":["31181348"]},"day":"01","publication_identifier":{"eissn":["1879-0410"],"issn":["0955-0674"]},"article_type":"original","title":"Multiscale dynamics of branching morphogenesis","date_updated":"2026-04-03T09:38:46Z","author":[{"orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"},{"full_name":"Simons, Benjamin D.","last_name":"Simons","first_name":"Benjamin D."}]},{"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.cell.2019.05.052"}],"oa_version":"Published Version","quality_controlled":"1","_id":"6601","volume":178,"department":[{"_id":"CaHe"},{"_id":"EdHa"}],"scopus_import":"1","doi":"10.1016/j.cell.2019.05.052","citation":{"ama":"Hannezo EB, Heisenberg C-PJ. Mechanochemical feedback loops in development and disease. <i>Cell</i>. 2019;178(1):12-25. doi:<a href=\"https://doi.org/10.1016/j.cell.2019.05.052\">10.1016/j.cell.2019.05.052</a>","ista":"Hannezo EB, Heisenberg C-PJ. 2019. Mechanochemical feedback loops in development and disease. Cell. 178(1), 12–25.","apa":"Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2019). Mechanochemical feedback loops in development and disease. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2019.05.052\">https://doi.org/10.1016/j.cell.2019.05.052</a>","ieee":"E. B. Hannezo and C.-P. J. Heisenberg, “Mechanochemical feedback loops in development and disease,” <i>Cell</i>, vol. 178, no. 1. Elsevier, pp. 12–25, 2019.","mla":"Hannezo, Edouard B., and Carl-Philipp J. Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” <i>Cell</i>, vol. 178, no. 1, Elsevier, 2019, pp. 12–25, doi:<a href=\"https://doi.org/10.1016/j.cell.2019.05.052\">10.1016/j.cell.2019.05.052</a>.","short":"E.B. Hannezo, C.-P.J. Heisenberg, Cell 178 (2019) 12–25.","chicago":"Hannezo, Edouard B, and Carl-Philipp J Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” <i>Cell</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.cell.2019.05.052\">https://doi.org/10.1016/j.cell.2019.05.052</a>."},"project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425","name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639"}],"author":[{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"date_updated":"2026-06-18T19:09:33Z","title":"Mechanochemical feedback loops in development and disease","publication_identifier":{"issn":["0092-8674"]},"article_type":"review","day":"27","external_id":{"isi":["000473002700005"],"pmid":["31251912"]},"issue":"1","ddc":["570"],"month":"07","status":"public","publication":"Cell","publisher":"Elsevier","pmid":1,"date_created":"2019-06-30T21:59:11Z","abstract":[{"text":"There is increasing evidence that both mechanical and biochemical signals play important roles in development and disease. The development of complex organisms, in particular, has been proposed to rely on the feedback between mechanical and biochemical patterning events. This feedback occurs at the molecular level via mechanosensation but can also arise as an emergent property of the system at the cellular and tissue level. In recent years, dynamic changes in tissue geometry, flow, rheology, and cell fate specification have emerged as key platforms of mechanochemical feedback loops in multiple processes. Here, we review recent experimental and theoretical advances in understanding how these feedbacks function in development and disease.","lang":"eng"}],"intvolume":"       178","language":[{"iso":"eng"}],"year":"2019","article_processing_charge":"No","publication_status":"published","date_published":"2019-07-27T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"12-25","oa":1,"isi":1,"ec_funded":1,"type":"journal_article"},{"day":"16","external_id":{"pmid":["31416964"],"isi":["000481688700050"]},"author":[{"first_name":"Denis","last_name":"Krndija","full_name":"Krndija, Denis"},{"full_name":"Marjou, Fatima El","last_name":"Marjou","first_name":"Fatima El"},{"last_name":"Guirao","first_name":"Boris","full_name":"Guirao, Boris"},{"full_name":"Richon, Sophie","last_name":"Richon","first_name":"Sophie"},{"full_name":"Leroy, Olivier","last_name":"Leroy","first_name":"Olivier"},{"full_name":"Bellaiche, Yohanns","first_name":"Yohanns","last_name":"Bellaiche"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"},{"full_name":"Vignjevic, Danijela Matic","last_name":"Vignjevic","first_name":"Danijela Matic"}],"title":"Active cell migration is critical for steady-state epithelial turnover in the gut","date_updated":"2023-08-29T07:16:40Z","volume":365,"scopus_import":"1","department":[{"_id":"EdHa"}],"citation":{"apa":"Krndija, D., Marjou, F. E., Guirao, B., Richon, S., Leroy, O., Bellaiche, Y., … Vignjevic, D. M. (2019). Active cell migration is critical for steady-state epithelial turnover in the gut. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aau3429\">https://doi.org/10.1126/science.aau3429</a>","ama":"Krndija D, Marjou FE, Guirao B, et al. Active cell migration is critical for steady-state epithelial turnover in the gut. <i>Science</i>. 2019;365(6454):705-710. doi:<a href=\"https://doi.org/10.1126/science.aau3429\">10.1126/science.aau3429</a>","ista":"Krndija D, Marjou FE, Guirao B, Richon S, Leroy O, Bellaiche Y, Hannezo EB, Vignjevic DM. 2019. Active cell migration is critical for steady-state epithelial turnover in the gut. Science. 365(6454), 705–710.","mla":"Krndija, Denis, et al. “Active Cell Migration Is Critical for Steady-State Epithelial Turnover in the Gut.” <i>Science</i>, vol. 365, no. 6454, American Association for the Advancement of Science, 2019, pp. 705–10, doi:<a href=\"https://doi.org/10.1126/science.aau3429\">10.1126/science.aau3429</a>.","ieee":"D. Krndija <i>et al.</i>, “Active cell migration is critical for steady-state epithelial turnover in the gut,” <i>Science</i>, vol. 365, no. 6454. American Association for the Advancement of Science, pp. 705–710, 2019.","short":"D. Krndija, F.E. Marjou, B. Guirao, S. Richon, O. Leroy, Y. Bellaiche, E.B. Hannezo, D.M. Vignjevic, Science 365 (2019) 705–710.","chicago":"Krndija, Denis, Fatima El Marjou, Boris Guirao, Sophie Richon, Olivier Leroy, Yohanns Bellaiche, Edouard B Hannezo, and Danijela Matic Vignjevic. “Active Cell Migration Is Critical for Steady-State Epithelial Turnover in the Gut.” <i>Science</i>. American Association for the Advancement of Science, 2019. <a href=\"https://doi.org/10.1126/science.aau3429\">https://doi.org/10.1126/science.aau3429</a>."},"doi":"10.1126/science.aau3429","quality_controlled":"1","oa_version":"None","_id":"6832","page":"705-710","type":"journal_article","isi":1,"publication_status":"published","year":"2019","article_processing_charge":"No","date_published":"2019-08-16T00:00:00Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"American Association for the Advancement of Science","date_created":"2019-08-25T22:00:51Z","pmid":1,"abstract":[{"text":"Steady-state turnover is a hallmark of epithelial tissues throughout adult life. Intestinal epithelial turnover is marked by continuous cell migration, which is assumed to be driven by mitotic pressure from the crypts. However, the balance of forces in renewal remains ill-defined. Combining biophysical modeling and quantitative three-dimensional tissue imaging with genetic and physical manipulations, we revealed the existence of an actin-related protein 2/3 complex–dependent active migratory force, which explains quantitatively the profiles of cell speed, density, and tissue tension along the villi. Cells migrate collectively with minimal rearrangements while displaying dual—apicobasal and front-back—polarity characterized by actin-rich basal protrusions oriented in the direction of migration. We propose that active migration is a critical component of gut epithelial turnover.","lang":"eng"}],"intvolume":"       365","language":[{"iso":"eng"}],"issue":"6454","month":"08","status":"public","publication":"Science"},{"related_material":{"record":[{"status":"public","relation":"used_in_publication","id":"7166"}]},"day":"19","type":"research_data_reference","author":[{"orcid":"0000-0003-0506-4217","last_name":"Ucar","first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425","full_name":"Ucar, Mehmet C"},{"first_name":"Reinhard","last_name":"Lipowsky","full_name":"Lipowsky, Reinhard"}],"article_processing_charge":"No","year":"2019","date_published":"2019-12-19T00:00:00Z","date_updated":"2026-07-06T12:13:40Z","title":"Supplementary information - Collective force generation by molecular motors is determined by strain-induced unbinding","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"American Chemical Society","date_created":"2021-07-27T09:51:46Z","department":[{"_id":"EdHa"}],"abstract":[{"text":"A detailed description of the two stochastic models, table of parameters, supplementary data for Figures 4 and 5, parameter dependence of the results, and an analysis on motors with different force–velocity functions (PDF)","lang":"eng"}],"doi":"10.1021/acs.nanolett.9b04445.s001","citation":{"short":"M.C. Ucar, R. Lipowsky, (2019).","chicago":"Ucar, Mehmet C, and Reinhard Lipowsky. “Supplementary Information - Collective Force Generation by Molecular Motors Is Determined by Strain-Induced Unbinding.” American Chemical Society, 2019. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s001\">https://doi.org/10.1021/acs.nanolett.9b04445.s001</a>.","ama":"Ucar MC, Lipowsky R. Supplementary information - Collective force generation by molecular motors is determined by strain-induced unbinding. 2019. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s001\">10.1021/acs.nanolett.9b04445.s001</a>","apa":"Ucar, M. C., &#38; Lipowsky, R. (2019). Supplementary information - Collective force generation by molecular motors is determined by strain-induced unbinding. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s001\">https://doi.org/10.1021/acs.nanolett.9b04445.s001</a>","ista":"Ucar MC, Lipowsky R. 2019. Supplementary information - Collective force generation by molecular motors is determined by strain-induced unbinding, American Chemical Society, <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s001\">10.1021/acs.nanolett.9b04445.s001</a>.","mla":"Ucar, Mehmet C., and Reinhard Lipowsky. <i>Supplementary Information - Collective Force Generation by Molecular Motors Is Determined by Strain-Induced Unbinding</i>. American Chemical Society, 2019, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s001\">10.1021/acs.nanolett.9b04445.s001</a>.","ieee":"M. C. Ucar and R. Lipowsky, “Supplementary information - Collective force generation by molecular motors is determined by strain-induced unbinding.” American Chemical Society, 2019."},"oa_version":"Published Version","month":"12","status":"public","_id":"9726"},{"author":[{"last_name":"Shamipour","first_name":"Shayan","full_name":"Shamipour, Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Roland","last_name":"Kardos","id":"4039350E-F248-11E8-B48F-1D18A9856A87","full_name":"Kardos, Roland"},{"id":"31D2C804-F248-11E8-B48F-1D18A9856A87","full_name":"Xue, Shi-lei","first_name":"Shi-lei","last_name":"Xue"},{"first_name":"Björn","orcid":"0000-0003-2057-2754","last_name":"Hof","id":"3A374330-F248-11E8-B48F-1D18A9856A87","full_name":"Hof, Björn"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","first_name":"Edouard B","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"}],"date_updated":"2026-09-02T22:30:19Z","title":"Bulk actin dynamics drive phase segregation in zebrafish oocytes","article_type":"original","publication_identifier":{"eissn":["1097-4172"],"issn":["0092-8674"]},"acknowledgement":"We would like to thank Pierre Recho, Guillaume Salbreux, and Silvia Grigolon for advice on the theory, Lila Solnica-Krezel for kindly providing us with zebrafish dachsous mutants, members of the Heisenberg and Hannezo groups for fruitful discussions, and the Bioimaging and zebrafish facilities at IST Austria for their continuous support. This project has received funding from the European Union (European Research Council Advanced Grant 742573 to C.P.H.) and from the Austrian Science Fund (FWF) (P 31639 to E.H.).","file_date_updated":"2020-10-21T07:22:34Z","related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/how-the-cytoplasm-separates-from-the-yolk/","description":"News on IST Homepage"}],"record":[{"status":"public","relation":"dissertation_contains","id":"8350"}]},"day":"30","external_id":{"isi":["000469415100013"],"pmid":["31080065"]},"_id":"6508","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.cell.2019.04.030"}],"quality_controlled":"1","oa_version":"Published Version","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"BjHo"}],"scopus_import":"1","volume":177,"project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"},{"grant_number":"P31639","name":"Active mechano-chemical description of the cell cytoskeleton","_id":"268294B6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"citation":{"chicago":"Shamipour, Shayan, Roland Kardos, Shi-lei Xue, Björn Hof, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” <i>Cell</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.cell.2019.04.030\">https://doi.org/10.1016/j.cell.2019.04.030</a>.","short":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E.B. Hannezo, C.-P.J. Heisenberg, Cell 177 (2019) 1463–1479.e18.","mla":"Shamipour, Shayan, et al. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” <i>Cell</i>, vol. 177, no. 6, Elsevier, 2019, p. 1463–1479.e18, doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.030\">10.1016/j.cell.2019.04.030</a>.","ieee":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E. B. Hannezo, and C.-P. J. Heisenberg, “Bulk actin dynamics drive phase segregation in zebrafish oocytes,” <i>Cell</i>, vol. 177, no. 6. Elsevier, p. 1463–1479.e18, 2019.","ista":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. 2019. Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. 177(6), 1463–1479.e18.","ama":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. Bulk actin dynamics drive phase segregation in zebrafish oocytes. <i>Cell</i>. 2019;177(6):1463-1479.e18. doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.030\">10.1016/j.cell.2019.04.030</a>","apa":"Shamipour, S., Kardos, R., Xue, S., Hof, B., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2019). Bulk actin dynamics drive phase segregation in zebrafish oocytes. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2019.04.030\">https://doi.org/10.1016/j.cell.2019.04.030</a>"},"doi":"10.1016/j.cell.2019.04.030","article_processing_charge":"No","year":"2019","publication_status":"published","file":[{"creator":"dernst","file_name":"2019_Cell_Shamipour_accepted.pdf","checksum":"aea43726d80e35ce3885073a5f05c3e3","relation":"main_file","date_created":"2020-10-21T07:22:34Z","content_type":"application/pdf","access_level":"open_access","file_size":3356292,"file_id":"8686","success":1,"date_updated":"2020-10-21T07:22:34Z"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2019-05-30T00:00:00Z","page":"1463-1479.e18","type":"journal_article","ec_funded":1,"isi":1,"oa":1,"issue":"6","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"ddc":["570"],"status":"public","month":"05","publication":"Cell","abstract":[{"text":"Segregation of maternal determinants within the oocyte constitutes the first step in embryo patterning. In zebrafish oocytes, extensive ooplasmic streaming leads to the segregation of ooplasm from yolk granules along the animal-vegetal axis of the oocyte. Here, we show that this process does not rely on cortical actin reorganization, as previously thought, but instead on a cell-cycle-dependent bulk actin polymerization wave traveling from the animal to the vegetal pole of the oocyte. This wave functions in segregation by both pulling ooplasm animally and pushing yolk granules vegetally. Using biophysical experimentation and theory, we show that ooplasm pulling is mediated by bulk actin network flows exerting friction forces on the ooplasm, while yolk granule pushing is achieved by a mechanism closely resembling actin comet formation on yolk granules. Our study defines a novel role of cell-cycle-controlled bulk actin polymerization waves in oocyte polarization via ooplasmic segregation.","lang":"eng"}],"date_created":"2019-06-02T21:59:12Z","has_accepted_license":"1","pmid":1,"publisher":"Elsevier","language":[{"iso":"eng"}],"intvolume":"       177"},{"publication":"Nature Cell Biology","status":"public","month":"05","issue":"6","intvolume":"        20","language":[{"iso":"eng"}],"publisher":"Nature Publishing Group","pmid":1,"abstract":[{"lang":"eng","text":"Recent lineage tracing studies have revealed that mammary gland homeostasis relies on unipotent stem cells. However, whether and when lineage restriction occurs during embryonic mammary development, and which signals orchestrate cell fate specification, remain unknown. Using a combination of in vivo clonal analysis with whole mount immunofluorescence and mathematical modelling of clonal dynamics, we found that embryonic multipotent mammary cells become lineage-restricted surprisingly early in development, with evidence for unipotency as early as E12.5 and no statistically discernable bipotency after E15.5. To gain insights into the mechanisms governing the switch from multipotency to unipotency, we used gain-of-function Notch1 mice and demonstrated that Notch activation cell autonomously dictates luminal cell fate specification to both embryonic and basally committed mammary cells. These functional studies have important implications for understanding the signals underlying cell plasticity and serve to clarify how reactivation of embryonic programs in adult cells can lead to cancer."}],"date_created":"2018-12-11T11:45:38Z","date_published":"2018-05-21T00:00:00Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_status":"published","year":"2018","article_processing_charge":"No","oa":1,"type":"journal_article","isi":1,"page":"677 - 687","quality_controlled":"1","oa_version":"Submitted Version","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984964"}],"_id":"288","publist_id":"7594","doi":"10.1038/s41556-018-0108-1","citation":{"ama":"Lilja A, Rodilla V, Huyghe M, et al. Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland. <i>Nature Cell Biology</i>. 2018;20(6):677-687. doi:<a href=\"https://doi.org/10.1038/s41556-018-0108-1\">10.1038/s41556-018-0108-1</a>","ista":"Lilja A, Rodilla V, Huyghe M, Hannezo EB, Landragin C, Renaud O, Leroy O, Rulands S, Simons B, Fré S. 2018. Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland. Nature Cell Biology. 20(6), 677–687.","apa":"Lilja, A., Rodilla, V., Huyghe, M., Hannezo, E. B., Landragin, C., Renaud, O., … Fré, S. (2018). Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41556-018-0108-1\">https://doi.org/10.1038/s41556-018-0108-1</a>","mla":"Lilja, Anna, et al. “Clonal Analysis of Notch1-Expressing Cells Reveals the Existence of Unipotent Stem Cells That Retain Long-Term Plasticity in the Embryonic Mammary Gland.” <i>Nature Cell Biology</i>, vol. 20, no. 6, Nature Publishing Group, 2018, pp. 677–87, doi:<a href=\"https://doi.org/10.1038/s41556-018-0108-1\">10.1038/s41556-018-0108-1</a>.","ieee":"A. Lilja <i>et al.</i>, “Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland,” <i>Nature Cell Biology</i>, vol. 20, no. 6. Nature Publishing Group, pp. 677–687, 2018.","short":"A. Lilja, V. Rodilla, M. Huyghe, E.B. Hannezo, C. Landragin, O. Renaud, O. Leroy, S. Rulands, B. Simons, S. Fré, Nature Cell Biology 20 (2018) 677–687.","chicago":"Lilja, Anna, Veronica Rodilla, Mathilde Huyghe, Edouard B Hannezo, Camille Landragin, Olivier Renaud, Olivier Leroy, Steffen Rulands, Benjamin Simons, and Silvia Fré. “Clonal Analysis of Notch1-Expressing Cells Reveals the Existence of Unipotent Stem Cells That Retain Long-Term Plasticity in the Embryonic Mammary Gland.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41556-018-0108-1\">https://doi.org/10.1038/s41556-018-0108-1</a>."},"volume":20,"scopus_import":"1","department":[{"_id":"EdHa"}],"title":"Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland","date_updated":"2023-09-11T12:44:08Z","author":[{"full_name":"Lilja, Anna","last_name":"Lilja","first_name":"Anna"},{"full_name":"Rodilla, Veronica","last_name":"Rodilla","first_name":"Veronica"},{"full_name":"Huyghe, Mathilde","first_name":"Mathilde","last_name":"Huyghe"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"full_name":"Landragin, Camille","last_name":"Landragin","first_name":"Camille"},{"last_name":"Renaud","first_name":"Olivier","full_name":"Renaud, Olivier"},{"first_name":"Olivier","last_name":"Leroy","full_name":"Leroy, Olivier"},{"full_name":"Rulands, Steffen","last_name":"Rulands","first_name":"Steffen"},{"full_name":"Simons, Benjamin","first_name":"Benjamin","last_name":"Simons"},{"full_name":"Fré, Silvia","first_name":"Silvia","last_name":"Fré"}],"external_id":{"isi":["000433237300003"],"pmid":["29784917"]},"day":"21","article_type":"original"},{"oa":1,"type":"journal_article","isi":1,"page":"360 - 375","date_published":"2018-08-06T00:00:00Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file":[{"file_id":"5694","date_updated":"2020-07-14T12:44:43Z","file_name":"2018_DevelopmentalCell_Sznurkowska.pdf","creator":"dernst","checksum":"78d2062b9e3c3b90fe71545aeb6d2f65","relation":"main_file","date_created":"2018-12-17T10:49:49Z","content_type":"application/pdf","access_level":"open_access","file_size":8948384}],"publication_status":"published","article_processing_charge":"No","year":"2018","intvolume":"        46","language":[{"iso":"eng"}],"publisher":"Cell Press","abstract":[{"text":"Pancreas development involves a coordinated process in which an early phase of cell segregation is followed by a longer phase of lineage restriction, expansion, and tissue remodeling. By combining clonal tracing and whole-mount reconstruction with proliferation kinetics and single-cell transcriptional profiling, we define the functional basis of pancreas morphogenesis. We show that the large-scale organization of mouse pancreas can be traced to the activity of self-renewing precursors positioned at the termini of growing ducts, which act collectively to drive serial rounds of stochastic ductal bifurcation balanced by termination. During this phase of branching morphogenesis, multipotent precursors become progressively fate-restricted, giving rise to self-renewing acinar-committed precursors that are conveyed with growing ducts, as well as ductal progenitors that expand the trailing ducts and give rise to delaminating endocrine cells. These findings define quantitatively how the functional behavior and lineage progression of precursor pools determine the large-scale patterning of pancreatic sub-compartments.","lang":"eng"}],"has_accepted_license":"1","date_created":"2018-12-11T11:44:48Z","status":"public","ddc":["570"],"month":"08","publication":"Developmental Cell","issue":"3","external_id":{"isi":["000441327300012"]},"day":"06","article_type":"original","file_date_updated":"2020-07-14T12:44:43Z","acknowledgement":"E.H. is funded by a Junior Research Fellowship from Trinity College, Cam-bridge, a Sir Henry Wellcome Fellowship from the Wellcome Trust, and theBettencourt-Schueller Young Researcher Prize for support.","title":"Defining lineage potential and fate behavior of precursors during pancreas development","date_updated":"2023-09-11T12:52:41Z","author":[{"last_name":"Sznurkowska","first_name":"Magdalena","full_name":"Sznurkowska, Magdalena"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"full_name":"Azzarelli, Roberta","last_name":"Azzarelli","first_name":"Roberta"},{"full_name":"Rulands, Steffen","last_name":"Rulands","first_name":"Steffen"},{"full_name":"Nestorowa, Sonia","last_name":"Nestorowa","first_name":"Sonia"},{"first_name":"Christopher","last_name":"Hindley","full_name":"Hindley, Christopher"},{"full_name":"Nichols, Jennifer","first_name":"Jennifer","last_name":"Nichols"},{"first_name":"Berthold","last_name":"Göttgens","full_name":"Göttgens, Berthold"},{"last_name":"Huch","first_name":"Meritxell","full_name":"Huch, Meritxell"},{"last_name":"Philpott","first_name":"Anna","full_name":"Philpott, Anna"},{"full_name":"Simons, Benjamin","last_name":"Simons","first_name":"Benjamin"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"doi":"10.1016/j.devcel.2018.06.028","citation":{"mla":"Sznurkowska, Magdalena, et al. “Defining Lineage Potential and Fate Behavior of Precursors during Pancreas Development.” <i>Developmental Cell</i>, vol. 46, no. 3, Cell Press, 2018, pp. 360–75, doi:<a href=\"https://doi.org/10.1016/j.devcel.2018.06.028\">10.1016/j.devcel.2018.06.028</a>.","ieee":"M. Sznurkowska <i>et al.</i>, “Defining lineage potential and fate behavior of precursors during pancreas development,” <i>Developmental Cell</i>, vol. 46, no. 3. Cell Press, pp. 360–375, 2018.","ama":"Sznurkowska M, Hannezo EB, Azzarelli R, et al. Defining lineage potential and fate behavior of precursors during pancreas development. <i>Developmental Cell</i>. 2018;46(3):360-375. doi:<a href=\"https://doi.org/10.1016/j.devcel.2018.06.028\">10.1016/j.devcel.2018.06.028</a>","ista":"Sznurkowska M, Hannezo EB, Azzarelli R, Rulands S, Nestorowa S, Hindley C, Nichols J, Göttgens B, Huch M, Philpott A, Simons B. 2018. Defining lineage potential and fate behavior of precursors during pancreas development. Developmental Cell. 46(3), 360–375.","apa":"Sznurkowska, M., Hannezo, E. B., Azzarelli, R., Rulands, S., Nestorowa, S., Hindley, C., … Simons, B. (2018). Defining lineage potential and fate behavior of precursors during pancreas development. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2018.06.028\">https://doi.org/10.1016/j.devcel.2018.06.028</a>","chicago":"Sznurkowska, Magdalena, Edouard B Hannezo, Roberta Azzarelli, Steffen Rulands, Sonia Nestorowa, Christopher Hindley, Jennifer Nichols, et al. “Defining Lineage Potential and Fate Behavior of Precursors during Pancreas Development.” <i>Developmental Cell</i>. Cell Press, 2018. <a href=\"https://doi.org/10.1016/j.devcel.2018.06.028\">https://doi.org/10.1016/j.devcel.2018.06.028</a>.","short":"M. Sznurkowska, E.B. Hannezo, R. Azzarelli, S. Rulands, S. Nestorowa, C. Hindley, J. Nichols, B. Göttgens, M. Huch, A. Philpott, B. Simons, Developmental Cell 46 (2018) 360–375."},"volume":46,"department":[{"_id":"EdHa"}],"scopus_import":"1","quality_controlled":"1","oa_version":"Published Version","_id":"132","publist_id":"7791"}]