[{"oa_version":"Submitted Version","month":"11","publisher":"Springer Nature","external_id":{"isi":["000720204300004"]},"acknowledgement":"S.G. acknowledges funding from FEDER Prostem Research Project no. 1510614 (Wallonia DG06), F.R.S.-FNRS Epiforce Research Project no. T.0092.21 and Interreg MAT(T)ISSE project, which is financially supported by Interreg France-Wallonie-Vlaanderen (Fonds Européen de Développement Régional, FEDER-ERDF). This project was supported by the European Research Council under the European Union’s Horizon 2020 Research and Innovation Programme grant agreement 851288 (to E.H.), and by the Austrian Science Fund (FWF) (P 31639; to E.H.). L.R.M. acknowledges funding from the Agence National de la Recherche (ANR), as part of the ‘Investments d’Avenir’ Programme (I-SITE ULNE/ANR-16-IDEX-0004 ULNE). This work benefited from ANR-10-EQPX-04-01 and FEDER 12001407 grants to F.L. W.D.V. is supported by the Research Foundation Flanders (FWO 1516619N, FWO GOO5819N, FWO I003420N, FWO IRI I000321N) and is member of the Research Excellence Consortium µNEURO at the University of Antwerp. M.L. is financially supported by FRIA (F.R.S.-FNRS). M.S. is a Senior Research Associate of the Fund for Scientific Research (F.R.S.-FNRS) and acknowledges EOS grant no. 30650939 (PRECISION). Sketches in Figs. 1a and 5e and Extended Data Fig. 9 were drawn by C. Levicek.","_id":"10365","department":[{"_id":"EdHa"}],"quality_controlled":"1","issue":"12","intvolume":"        17","volume":17,"article_type":"original","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"author":[{"first_name":"Marine","last_name":"Luciano","full_name":"Luciano, Marine"},{"first_name":"Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","last_name":"Xue","full_name":"Xue, Shi-lei"},{"full_name":"De Vos, Winnok H.","first_name":"Winnok H.","last_name":"De Vos"},{"full_name":"Redondo-Morata, Lorena","first_name":"Lorena","last_name":"Redondo-Morata"},{"full_name":"Surin, Mathieu","last_name":"Surin","first_name":"Mathieu"},{"last_name":"Lafont","first_name":"Frank","full_name":"Lafont, Frank"},{"last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"first_name":"Sylvain","last_name":"Gabriele","full_name":"Gabriele, Sylvain"}],"date_updated":"2025-04-14T07:52:26Z","date_created":"2021-11-28T23:01:29Z","year":"2021","citation":{"apa":"Luciano, M., Xue, S., De Vos, W. H., Redondo-Morata, L., Surin, M., Lafont, F., … Gabriele, S. (2021). Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-021-01374-1\">https://doi.org/10.1038/s41567-021-01374-1</a>","ista":"Luciano M, Xue S, De Vos WH, Redondo-Morata L, Surin M, Lafont F, Hannezo EB, Gabriele S. 2021. Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation. Nature Physics. 17(12), 1382–1390.","ama":"Luciano M, Xue S, De Vos WH, et al. Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation. <i>Nature Physics</i>. 2021;17(12):1382–1390. doi:<a href=\"https://doi.org/10.1038/s41567-021-01374-1\">10.1038/s41567-021-01374-1</a>","ieee":"M. Luciano <i>et al.</i>, “Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation,” <i>Nature Physics</i>, vol. 17, no. 12. Springer Nature, pp. 1382–1390, 2021.","short":"M. Luciano, S. Xue, W.H. De Vos, L. Redondo-Morata, M. Surin, F. Lafont, E.B. Hannezo, S. Gabriele, Nature Physics 17 (2021) 1382–1390.","mla":"Luciano, Marine, et al. “Cell Monolayers Sense Curvature by Exploiting Active Mechanics and Nuclear Mechanoadaptation.” <i>Nature Physics</i>, vol. 17, no. 12, Springer Nature, 2021, pp. 1382–1390, doi:<a href=\"https://doi.org/10.1038/s41567-021-01374-1\">10.1038/s41567-021-01374-1</a>.","chicago":"Luciano, Marine, Shi-lei Xue, Winnok H. De Vos, Lorena Redondo-Morata, Mathieu Surin, Frank Lafont, Edouard B Hannezo, and Sylvain Gabriele. “Cell Monolayers Sense Curvature by Exploiting Active Mechanics and Nuclear Mechanoadaptation.” <i>Nature Physics</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41567-021-01374-1\">https://doi.org/10.1038/s41567-021-01374-1</a>."},"publication":"Nature Physics","abstract":[{"lang":"eng","text":"The early development of many organisms involves the folding of cell monolayers, but this behaviour is difficult to reproduce in vitro; therefore, both mechanistic causes and effects of local curvature remain unclear. Here we study epithelial cell monolayers on corrugated hydrogels engineered into wavy patterns, examining how concave and convex curvatures affect cellular and nuclear shape. We find that substrate curvature affects monolayer thickness, which is larger in valleys than crests. We show that this feature generically arises in a vertex model, leading to the hypothesis that cells may sense curvature by modifying the thickness of the tissue. We find that local curvature also affects nuclear morphology and positioning, which we explain by extending the vertex model to take into account membrane–nucleus interactions, encoding thickness modulation in changes to nuclear deformation and position. We propose that curvature governs the spatial distribution of yes-associated proteins via nuclear shape and density changes. We show that curvature also induces significant variations in lamins, chromatin condensation and cell proliferation rate in folded epithelial tissues. Together, this work identifies active cell mechanics and nuclear mechanoadaptation as the key players of the mechanistic regulation of epithelia to substrate curvature."}],"scopus_import":"1","doi":"10.1038/s41567-021-01374-1","day":"18","date_published":"2021-11-18T00:00:00Z","oa":1,"related_material":{"link":[{"url":"https://ist.ac.at/en/news/how-cells-feel-curvature/","relation":"press_release","description":"News on IST Webpage"}]},"article_processing_charge":"No","file_date_updated":"2023-10-11T09:31:43Z","has_accepted_license":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"1382–1390","language":[{"iso":"eng"}],"corr_author":"1","status":"public","ddc":["530"],"ec_funded":1,"type":"journal_article","file":[{"relation":"main_file","success":1,"date_updated":"2023-10-11T09:31:43Z","file_name":"50145_4_merged_1630498627.pdf","access_level":"open_access","date_created":"2023-10-11T09:31:43Z","content_type":"application/pdf","file_id":"14420","file_size":40285498,"checksum":"5d6d76750a71d7cb632bb15417c38ef7","creator":"channezo"}],"project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis"},{"call_identifier":"FWF","name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639","_id":"268294B6-B435-11E9-9278-68D0E5697425"}],"title":"Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation","publication_status":"published","isi":1,"fulldoi":"https://doi.org/10.1038/s41567-021-01374-1"},{"pmid":1,"article_number":"6830","quality_controlled":"1","publication_identifier":{"eissn":["2041-1723"]},"article_type":"original","volume":12,"intvolume":"        12","external_id":{"pmid":["34819507"],"isi":["000722322900020"]},"publisher":"Springer Nature","oa_version":"Published Version","month":"11","department":[{"_id":"EdHa"}],"_id":"10402","acknowledgement":"We thank all members of our respective groups for helpful discussion on the paper. The authors are also grateful to Prof. Abdel El. Manira for support and sharing Tg(HUC:Gal4;UAS:Synaptohysin-GFP), to Haohao Wu for discussion, and thank Elena Zabalueva for the zebrafish schematic. The authors also acknowledge Zebrafish core facility, Genome Engineering Zebrafish and Biomedicum Imaging Core from the Karolinska Institutet for technical support. This work received funding from the ERC under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 851288 to E.H.) and under the Marie Skłodowska-Curie grant agreement No. 754411 (to M.C.U.); Swedish Research Council (to F.L., I.A. and S.H.); Knut and Alice Wallenberg Foundation (F.L. and I.A.); Swedish Brain Foundation (F.L. and S.H.); Ming Wai Lau Foundation (to F.L.); StratRegen (to F.L.); ERC Consolidator grant STEMMING-FROM-NERVE and ERC Synergy Grant KILL-OR-DIFFERENTIATE (to I.A.); Bertil Hallsten Research Foundation (to I.A.); Cancerfonden (to I.A.); the Paradifference Foundation (to I.A.); Austrian Science Fund (to I.A.); and StratNeuro (to S.H.).","day":"24","date_published":"2021-11-24T00:00:00Z","doi":"10.1038/s41467-021-27135-5","abstract":[{"text":"Branching morphogenesis governs the formation of many organs such as lung, kidney, and the neurovascular system. Many studies have explored system-specific molecular and cellular regulatory mechanisms, as well as self-organizing rules underlying branching morphogenesis. However, in addition to local cues, branched tissue growth can also be influenced by global guidance. Here, we develop a theoretical framework for a stochastic self-organized branching process in the presence of external cues. Combining analytical theory with numerical simulations, we predict differential signatures of global vs. local regulatory mechanisms on the branching pattern, such as angle distributions, domain size, and space-filling efficiency. We find that branch alignment follows a generic scaling law determined by the strength of global guidance, while local interactions influence the tissue density but not its overall territory. Finally, using zebrafish innervation as a model system, we test these key features of the model experimentally. Our work thus provides quantitative predictions to disentangle the role of different types of cues in shaping branched structures across scales.","lang":"eng"}],"scopus_import":"1","date_updated":"2025-04-14T07:43:47Z","author":[{"orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C","last_name":"Ucar","first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425"},{"full_name":"Kamenev, Dmitrii","last_name":"Kamenev","first_name":"Dmitrii"},{"last_name":"Sunadome","first_name":"Kazunori","full_name":"Sunadome, Kazunori"},{"first_name":"Dominik C","id":"14FDD550-AA41-11E9-A0E5-1ACCE5697425","last_name":"Fachet","full_name":"Fachet, Dominik C"},{"last_name":"Lallemend","first_name":"Francois","full_name":"Lallemend, Francois"},{"full_name":"Adameyko, Igor","first_name":"Igor","last_name":"Adameyko"},{"first_name":"Saida","last_name":"Hadjab","full_name":"Hadjab, Saida"},{"last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"}],"publication":"Nature Communications","citation":{"mla":"Ucar, Mehmet C., et al. “Theory of Branching Morphogenesis by Local Interactions and Global Guidance.” <i>Nature Communications</i>, vol. 12, 6830, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-27135-5\">10.1038/s41467-021-27135-5</a>.","short":"M.C. Ucar, D. Kamenev, K. Sunadome, D.C. Fachet, F. Lallemend, I. Adameyko, S. Hadjab, E.B. Hannezo, Nature Communications 12 (2021).","ieee":"M. C. Ucar <i>et al.</i>, “Theory of branching morphogenesis by local interactions and global guidance,” <i>Nature Communications</i>, vol. 12. Springer Nature, 2021.","chicago":"Ucar, Mehmet C, Dmitrii Kamenev, Kazunori Sunadome, Dominik C Fachet, Francois Lallemend, Igor Adameyko, Saida Hadjab, and Edouard B Hannezo. “Theory of Branching Morphogenesis by Local Interactions and Global Guidance.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-27135-5\">https://doi.org/10.1038/s41467-021-27135-5</a>.","apa":"Ucar, M. C., Kamenev, D., Sunadome, K., Fachet, D. C., Lallemend, F., Adameyko, I., … Hannezo, E. B. (2021). Theory of branching morphogenesis by local interactions and global guidance. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-27135-5\">https://doi.org/10.1038/s41467-021-27135-5</a>","ama":"Ucar MC, Kamenev D, Sunadome K, et al. Theory of branching morphogenesis by local interactions and global guidance. <i>Nature Communications</i>. 2021;12. doi:<a href=\"https://doi.org/10.1038/s41467-021-27135-5\">10.1038/s41467-021-27135-5</a>","ista":"Ucar MC, Kamenev D, Sunadome K, Fachet DC, Lallemend F, Adameyko I, Hadjab S, Hannezo EB. 2021. Theory of branching morphogenesis by local interactions and global guidance. Nature Communications. 12, 6830."},"year":"2021","date_created":"2021-12-05T23:01:40Z","license":"https://creativecommons.org/licenses/by/4.0/","file_date_updated":"2021-12-10T08:54:09Z","has_accepted_license":"1","article_processing_charge":"No","related_material":{"record":[{"relation":"research_data","id":"13058","status":"public"}]},"oa":1,"language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","file":[{"file_name":"2021_NatComm_Ucar.pdf","access_level":"open_access","content_type":"application/pdf","date_created":"2021-12-10T08:54:09Z","success":1,"date_updated":"2021-12-10T08:54:09Z","relation":"main_file","checksum":"63c56ec75314a71e63e7dd2920b3c5b5","creator":"cchlebak","file_size":2303405,"file_id":"10529"}],"project":[{"call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E"},{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425"}],"title":"Theory of branching morphogenesis by local interactions and global guidance","fulldoi":"https://doi.org/10.1038/s41467-021-27135-5","isi":1,"status":"public","type":"journal_article","ddc":["573"],"ec_funded":1},{"issue":"26","quality_controlled":"1","publication_identifier":{"issn":["0092-8674"],"eissn":["1097-4172"]},"article_type":"original","volume":184,"intvolume":"       184","external_id":{"isi":["000735387500002"]},"publisher":"Elsevier","oa_version":"Preprint","month":"12","department":[{"_id":"EdHa"}],"_id":"10573","acknowledgement":"We thank Ian Swinburne, Sandy Nandagopal, and Toru Kawanishi for support, discussions, and reagents. We thank Vanessa Barone, Joseph Nasser, and members of the Megason lab for useful comments on the manuscript and general feedback. We are grateful to the Heisenberg and Knaut labs for transgenic fish. Diagrams on the right in the graphical abstract were created using BioRender. This work was supported by NIH R01DC015478 and NIH R01GM107733 to S.G.M. A.M. was supported by Human Frontiers Science Program LTF and NIH K99HD098918.","day":"22","date_published":"2021-12-22T00:00:00Z","doi":"10.1016/j.cell.2021.11.025","abstract":[{"text":"How tissues acquire complex shapes is a fundamental question in biology and regenerative medicine. Zebrafish semicircular canals form from invaginations in the otic epithelium (buds) that extend and fuse to form the hubs of each canal. We find that conventional actomyosin-driven behaviors are not required. Instead, local secretion of hyaluronan, made by the enzymes uridine 5′-diphosphate dehydrogenase (ugdh) and hyaluronan synthase 3 (has3), drives canal morphogenesis. Charged hyaluronate polymers osmotically swell with water and generate isotropic extracellular pressure to deform the overlying epithelium into buds. The mechanical anisotropy needed to shape buds into tubes is conferred by a polarized distribution of actomyosin and E-cadherin-rich membrane tethers, which we term cytocinches. Most work on tissue morphogenesis ascribes actomyosin contractility as the driving force, while the extracellular matrix shapes tissues through differential stiffness. Our work inverts this expectation. Hyaluronate pressure shaped by anisotropic tissue stiffness may be a widespread mechanism for powering morphological change in organogenesis and tissue engineering.","lang":"eng"}],"scopus_import":"1","date_updated":"2025-05-14T11:26:20Z","author":[{"last_name":"Munjal","first_name":"Akankshi","full_name":"Munjal, Akankshi"},{"last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"full_name":"Tsai, Tony Y.C.","first_name":"Tony Y.C.","last_name":"Tsai"},{"full_name":"Mitchison, Timothy J.","first_name":"Timothy J.","last_name":"Mitchison"},{"full_name":"Megason, Sean G.","first_name":"Sean G.","last_name":"Megason"}],"publication":"Cell","main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/2020.09.28.316042"}],"citation":{"chicago":"Munjal, Akankshi, Edouard B Hannezo, Tony Y.C. Tsai, Timothy J. Mitchison, and Sean G. Megason. “Extracellular Hyaluronate Pressure Shaped by Cellular Tethers Drives Tissue Morphogenesis.” <i>Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.cell.2021.11.025\">https://doi.org/10.1016/j.cell.2021.11.025</a>.","short":"A. Munjal, E.B. Hannezo, T.Y.C. Tsai, T.J. Mitchison, S.G. Megason, Cell 184 (2021) 6313–6325.e18.","ieee":"A. Munjal, E. B. Hannezo, T. Y. C. Tsai, T. J. Mitchison, and S. G. Megason, “Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis,” <i>Cell</i>, vol. 184, no. 26. Elsevier, p. 6313–6325.e18, 2021.","mla":"Munjal, Akankshi, et al. “Extracellular Hyaluronate Pressure Shaped by Cellular Tethers Drives Tissue Morphogenesis.” <i>Cell</i>, vol. 184, no. 26, Elsevier, 2021, p. 6313–6325.e18, doi:<a href=\"https://doi.org/10.1016/j.cell.2021.11.025\">10.1016/j.cell.2021.11.025</a>.","ama":"Munjal A, Hannezo EB, Tsai TYC, Mitchison TJ, Megason SG. Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis. <i>Cell</i>. 2021;184(26):6313-6325.e18. doi:<a href=\"https://doi.org/10.1016/j.cell.2021.11.025\">10.1016/j.cell.2021.11.025</a>","ista":"Munjal A, Hannezo EB, Tsai TYC, Mitchison TJ, Megason SG. 2021. Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis. Cell. 184(26), 6313–6325.e18.","apa":"Munjal, A., Hannezo, E. B., Tsai, T. Y. C., Mitchison, T. J., &#38; Megason, S. G. (2021). Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2021.11.025\">https://doi.org/10.1016/j.cell.2021.11.025</a>"},"date_created":"2021-12-26T23:01:26Z","year":"2021","article_processing_charge":"No","oa":1,"language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"6313-6325.e18","publication_status":"published","title":"Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis","fulldoi":"https://doi.org/10.1016/j.cell.2021.11.025","isi":1,"status":"public","type":"journal_article"},{"oa":1,"has_accepted_license":"1","file_date_updated":"2021-03-22T08:50:33Z","article_processing_charge":"No","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","status":"public","ec_funded":1,"ddc":["570"],"type":"journal_article","publication_status":"published","file":[{"checksum":"20ccf4dfe46c48cf986794c8bf4fd1cb","creator":"dernst","file_size":9259690,"file_id":"9271","file_name":"2021_eLife_Hankeova.pdf","access_level":"open_access","date_created":"2021-03-22T08:50:33Z","content_type":"application/pdf","success":1,"date_updated":"2021-03-22T08:50:33Z","relation":"main_file"}],"title":"DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome","project":[{"name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","call_identifier":"H2020"}],"isi":1,"fulldoi":"https://doi.org/10.7554/eLife.60916","publisher":"eLife Sciences Publications","oa_version":"Published Version","month":"02","external_id":{"pmid":["33635272"],"isi":["000625357100001"]},"acknowledgement":"Work in ERA lab is supported by the Swedish Research Council, the Center of Innovative Medicine (CIMED) Grant, Karolinska Institutet, and the Heart and Lung Foundation, and\r\nthe Daniel Alagille Award from the European Association for the Study of the Liver. One project in ERA lab is funded by ModeRNA, unrelated to this project. The funders have no role in the design or interpretation of the work. SH has been supported by a KI-MU PhD student program, and by a Wera Ekstro¨m Foundation Scholarship. We are grateful for support from Tornspiran foundation to NVH. JK: This research was carried out under the project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II and CzechNanoLab Research Infrastructure supported by MEYS CR (LM2018110) . UL: The financial support from the Swedish Research Council and ICMC (Integrated CardioMetabolic Center) is acknowledged. JJ: The work was supported by the Grant Agency of Masaryk University (project no. MUNI/A/1565/2018). We thank Kari Huppert and Stacey Huppert for their expertise and help regarding bile duct cannulation and their laboratory hospitality. We also thank Nadja Schultz and Charlotte L Mattsson for their help with common bile duct cannulation. We thank Daniel Holl for his help with trachea cannulation. We thank Nikos Papadogiannakis for his assistance with mild Alagille biopsy samples and discussion. We thank Karolinska Biomedicum Imaging Core, especially Shigeaki Kanatani for his help with image analysis. We thank Jan Masek and Carolina Gutierrez for their scientific input in manuscript writing. We thank Peter Ranefall and the BioImage Informatics (SciLife national facility) for their help writing parts of the MATLAB pipeline.\r\nThe TROMA-III antibody developed by Rolf Kemler was obtained from the Developmental Studies Hybridoma (DSHB) Bank developed under the auspices of NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA52242. We thank Goncalo M Brito for all illustrations. This work was supported by the European Union (European Research Council Starting grant 851288 to E.H.).","department":[{"_id":"EdHa"}],"_id":"9244","quality_controlled":"1","article_number":"e60916","pmid":1,"intvolume":"        10","publication_identifier":{"eissn":["2050-084X"]},"article_type":"original","volume":10,"author":[{"last_name":"Hankeova","first_name":"Simona","full_name":"Hankeova, Simona"},{"last_name":"Salplachta","first_name":"Jakub","full_name":"Salplachta, Jakub"},{"first_name":"Tomas","last_name":"Zikmund","full_name":"Zikmund, Tomas"},{"full_name":"Kavkova, Michaela","last_name":"Kavkova","first_name":"Michaela"},{"first_name":"Noémi","last_name":"Van Hul","full_name":"Van Hul, Noémi"},{"first_name":"Adam","last_name":"Brinek","full_name":"Brinek, Adam"},{"full_name":"Smekalova, Veronika","first_name":"Veronika","last_name":"Smekalova"},{"full_name":"Laznovsky, Jakub","last_name":"Laznovsky","first_name":"Jakub"},{"full_name":"Dawit, Feven","first_name":"Feven","last_name":"Dawit"},{"full_name":"Jaros, Josef","last_name":"Jaros","first_name":"Josef"},{"last_name":"Bryja","first_name":"Vítězslav","full_name":"Bryja, Vítězslav"},{"first_name":"Urban","last_name":"Lendahl","full_name":"Lendahl, Urban"},{"full_name":"Ellis, Ewa","last_name":"Ellis","first_name":"Ewa"},{"last_name":"Nemeth","first_name":"Antal","full_name":"Nemeth, Antal"},{"full_name":"Fischler, Björn","first_name":"Björn","last_name":"Fischler"},{"last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"full_name":"Kaiser, Jozef","first_name":"Jozef","last_name":"Kaiser"},{"last_name":"Andersson","first_name":"Emma Rachel","full_name":"Andersson, Emma Rachel"}],"date_updated":"2026-04-02T14:00:00Z","citation":{"apa":"Hankeova, S., Salplachta, J., Zikmund, T., Kavkova, M., Van Hul, N., Brinek, A., … Andersson, E. R. (2021). DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.60916\">https://doi.org/10.7554/eLife.60916</a>","ama":"Hankeova S, Salplachta J, Zikmund T, et al. DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome. <i>eLife</i>. 2021;10. doi:<a href=\"https://doi.org/10.7554/eLife.60916\">10.7554/eLife.60916</a>","ista":"Hankeova S, Salplachta J, Zikmund T, Kavkova M, Van Hul N, Brinek A, Smekalova V, Laznovsky J, Dawit F, Jaros J, Bryja V, Lendahl U, Ellis E, Nemeth A, Fischler B, Hannezo EB, Kaiser J, Andersson ER. 2021. DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome. eLife. 10, e60916.","short":"S. Hankeova, J. Salplachta, T. Zikmund, M. Kavkova, N. Van Hul, A. Brinek, V. Smekalova, J. Laznovsky, F. Dawit, J. Jaros, V. Bryja, U. Lendahl, E. Ellis, A. Nemeth, B. Fischler, E.B. Hannezo, J. Kaiser, E.R. Andersson, ELife 10 (2021).","ieee":"S. Hankeova <i>et al.</i>, “DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome,” <i>eLife</i>, vol. 10. eLife Sciences Publications, 2021.","mla":"Hankeova, Simona, et al. “DUCT Reveals Architectural Mechanisms Contributing to Bile Duct Recovery in a Mouse Model for Alagille Syndrome.” <i>ELife</i>, vol. 10, e60916, eLife Sciences Publications, 2021, doi:<a href=\"https://doi.org/10.7554/eLife.60916\">10.7554/eLife.60916</a>.","chicago":"Hankeova, Simona, Jakub Salplachta, Tomas Zikmund, Michaela Kavkova, Noémi Van Hul, Adam Brinek, Veronika Smekalova, et al. “DUCT Reveals Architectural Mechanisms Contributing to Bile Duct Recovery in a Mouse Model for Alagille Syndrome.” <i>ELife</i>. eLife Sciences Publications, 2021. <a href=\"https://doi.org/10.7554/eLife.60916\">https://doi.org/10.7554/eLife.60916</a>."},"year":"2021","date_created":"2021-03-14T23:01:34Z","publication":"eLife","scopus_import":"1","abstract":[{"lang":"eng","text":"Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models."}],"date_published":"2021-02-26T00:00:00Z","day":"26","doi":"10.7554/eLife.60916"},{"publication":"Journal of Cell Biology","date_created":"2021-04-04T22:01:21Z","year":"2021","citation":{"ista":"Dobramysl U, Jarsch IK, Inoue Y, Shimo H, Richier B, Gadsby JR, Mason J, Szałapak A, Ioannou PS, Correia GP, Walrant A, Butler R, Hannezo EB, Simons BD, Gallop JL. 2021. Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation. Journal of Cell Biology. 220(4), e202003052.","ama":"Dobramysl U, Jarsch IK, Inoue Y, et al. Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation. <i>Journal of Cell Biology</i>. 2021;220(4). doi:<a href=\"https://doi.org/10.1083/jcb.202003052\">10.1083/jcb.202003052</a>","apa":"Dobramysl, U., Jarsch, I. K., Inoue, Y., Shimo, H., Richier, B., Gadsby, J. R., … Gallop, J. L. (2021). Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202003052\">https://doi.org/10.1083/jcb.202003052</a>","chicago":"Dobramysl, Ulrich, Iris Katharina Jarsch, Yoshiko Inoue, Hanae Shimo, Benjamin Richier, Jonathan R. Gadsby, Julia Mason, et al. “Stochastic Combinations of Actin Regulatory Proteins Are Sufficient to Drive Filopodia Formation.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2021. <a href=\"https://doi.org/10.1083/jcb.202003052\">https://doi.org/10.1083/jcb.202003052</a>.","mla":"Dobramysl, Ulrich, et al. “Stochastic Combinations of Actin Regulatory Proteins Are Sufficient to Drive Filopodia Formation.” <i>Journal of Cell Biology</i>, vol. 220, no. 4, e202003052, Rockefeller University Press, 2021, doi:<a href=\"https://doi.org/10.1083/jcb.202003052\">10.1083/jcb.202003052</a>.","ieee":"U. Dobramysl <i>et al.</i>, “Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation,” <i>Journal of Cell Biology</i>, vol. 220, no. 4. Rockefeller University Press, 2021.","short":"U. Dobramysl, I.K. Jarsch, Y. Inoue, H. Shimo, B. Richier, J.R. Gadsby, J. Mason, A. Szałapak, P.S. Ioannou, G.P. Correia, A. Walrant, R. Butler, E.B. Hannezo, B.D. Simons, J.L. Gallop, Journal of Cell Biology 220 (2021)."},"date_updated":"2026-04-02T13:59:43Z","author":[{"first_name":"Ulrich","last_name":"Dobramysl","full_name":"Dobramysl, Ulrich"},{"full_name":"Jarsch, Iris Katharina","last_name":"Jarsch","first_name":"Iris Katharina"},{"first_name":"Yoshiko","last_name":"Inoue","full_name":"Inoue, Yoshiko"},{"full_name":"Shimo, Hanae","last_name":"Shimo","first_name":"Hanae"},{"last_name":"Richier","first_name":"Benjamin","full_name":"Richier, Benjamin"},{"full_name":"Gadsby, Jonathan R.","last_name":"Gadsby","first_name":"Jonathan R."},{"last_name":"Mason","first_name":"Julia","full_name":"Mason, Julia"},{"first_name":"Alicja","last_name":"Szałapak","full_name":"Szałapak, Alicja"},{"last_name":"Ioannou","first_name":"Pantelis Savvas","full_name":"Ioannou, Pantelis Savvas"},{"full_name":"Correia, Guilherme Pereira","first_name":"Guilherme Pereira","last_name":"Correia"},{"last_name":"Walrant","first_name":"Astrid","full_name":"Walrant, Astrid"},{"full_name":"Butler, Richard","first_name":"Richard","last_name":"Butler"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"last_name":"Simons","first_name":"Benjamin D.","full_name":"Simons, Benjamin D."},{"full_name":"Gallop, Jennifer L.","last_name":"Gallop","first_name":"Jennifer L."}],"doi":"10.1083/jcb.202003052","date_published":"2021-03-19T00:00:00Z","day":"19","abstract":[{"lang":"eng","text":"Assemblies of actin and its regulators underlie the dynamic morphology of all eukaryotic cells. To understand how actin regulatory proteins work together to generate actin-rich structures such as filopodia, we analyzed the localization of diverse actin regulators within filopodia in Drosophila embryos and in a complementary in vitro system of filopodia-like structures (FLSs). We found that the composition of the regulatory protein complex where actin is incorporated (the filopodial tip complex) is remarkably heterogeneous both in vivo and in vitro. Our data reveal that different pairs of proteins correlate with each other and with actin bundle length, suggesting the presence of functional subcomplexes. This is consistent with a theoretical framework where three or more redundant subcomplexes join the tip complex stochastically, with any two being sufficient to drive filopodia formation. We provide an explanation for the observed heterogeneity and suggest that a mechanism based on multiple components allows stereotypical filopodial dynamics to arise from diverse upstream signaling pathways."}],"scopus_import":"1","_id":"9306","department":[{"_id":"EdHa"}],"acknowledgement":"This work was supported by European Research Council grant 281971, Wellcome Trust Research Career Development Fellowship WT095829AIA and Wellcome Trust Senior Research\r\nFellowship 219482/Z/19/Z to J.L. Gallop, a Wellcome Trust Senior Investigator Award 098357 to B.D. Simons, and an Austrian Science Fund grant (P31639) to E. Hannezo. We acknowledge\r\ncore funding by the Wellcome Trust (092096) and Cancer Research UK (C6946/A14492). U. Dobramysl was supported by a Wellcome Trust Junior Interdisciplinary Fellowship grant\r\n(105602/Z/14/Z) and a Herchel Smith Postdoctoral Fellowship. H. Shimo was supported by a Funai Foundation Overseas scholarship.","external_id":{"pmid":["33740033"],"isi":["000663160600002"]},"month":"03","oa_version":"Published Version","publisher":"Rockefeller University Press","volume":220,"publication_identifier":{"eissn":["1540-8140"]},"article_type":"original","intvolume":"       220","issue":"4","article_number":"e202003052","pmid":1,"quality_controlled":"1","type":"journal_article","ddc":["576"],"status":"public","fulldoi":"https://doi.org/10.1083/jcb.202003052","isi":1,"file":[{"creator":"dernst","checksum":"4739ffd90f2c7e05ac5b00f057c58aa2","file_id":"9310","file_size":9019720,"date_created":"2021-04-06T10:39:08Z","content_type":"application/pdf","access_level":"open_access","file_name":"2021_JCB_Dobramysl.pdf","relation":"main_file","date_updated":"2021-04-06T10:39:08Z","success":1}],"title":"Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation","project":[{"name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639","_id":"268294B6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"publication_status":"published","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"language":[{"iso":"eng"}],"article_processing_charge":"No","has_accepted_license":"1","file_date_updated":"2021-04-06T10:39:08Z","oa":1},{"acknowledgement":"We thank Carl Goodrich and the members of the Heisenberg and Hannezo groups, in particular Reka Korei, for help, technical advice, and discussions; and the Bioimaging and zebrafish facilities of the IST Austria for continuous support. This work was supported by the Elise Richter Program of Austrian Science Fund (FWF) to N.I.P. ( V 736-B26 ) and the European Union (European Research Council Advanced Grant 742573 to C.-P.H. and European Research Council Starting Grant 851288 to E.H.).","_id":"9316","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"month":"04","oa_version":"Published Version","publisher":"Elsevier","external_id":{"pmid":["33730596"],"isi":["000636734000022"]},"intvolume":"       184","volume":184,"article_type":"original","publication_identifier":{"eissn":["1097-4172"],"issn":["0092-8674"]},"quality_controlled":"1","pmid":1,"issue":"7","date_created":"2021-04-11T22:01:14Z","year":"2021","citation":{"chicago":"Petridou, Nicoletta, Bernat Corominas-Murtra, Carl-Philipp J Heisenberg, and Edouard B Hannezo. “Rigidity Percolation Uncovers a Structural Basis for Embryonic Tissue Phase Transitions.” <i>Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.cell.2021.02.017\">https://doi.org/10.1016/j.cell.2021.02.017</a>.","mla":"Petridou, Nicoletta, et al. “Rigidity Percolation Uncovers a Structural Basis for Embryonic Tissue Phase Transitions.” <i>Cell</i>, vol. 184, no. 7, Elsevier, 2021, p. 1914–1928.e19, doi:<a href=\"https://doi.org/10.1016/j.cell.2021.02.017\">10.1016/j.cell.2021.02.017</a>.","short":"N. Petridou, B. Corominas-Murtra, C.-P.J. Heisenberg, E.B. Hannezo, Cell 184 (2021) 1914–1928.e19.","ieee":"N. Petridou, B. Corominas-Murtra, C.-P. J. Heisenberg, and E. B. Hannezo, “Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions,” <i>Cell</i>, vol. 184, no. 7. Elsevier, p. 1914–1928.e19, 2021.","ista":"Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. 2021. Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. Cell. 184(7), 1914–1928.e19.","ama":"Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. <i>Cell</i>. 2021;184(7):1914-1928.e19. doi:<a href=\"https://doi.org/10.1016/j.cell.2021.02.017\">10.1016/j.cell.2021.02.017</a>","apa":"Petridou, N., Corominas-Murtra, B., Heisenberg, C.-P. J., &#38; Hannezo, E. B. (2021). Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2021.02.017\">https://doi.org/10.1016/j.cell.2021.02.017</a>"},"publication":"Cell","author":[{"last_name":"Petridou","first_name":"Nicoletta","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8451-1195","full_name":"Petridou, Nicoletta"},{"last_name":"Corominas-Murtra","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","first_name":"Bernat","orcid":"0000-0001-9806-5643","full_name":"Corominas-Murtra, Bernat"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"}],"date_updated":"2025-07-10T12:01:42Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"abstract":[{"text":"Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural PTs of embryonic tissues from local cell connectivity. By using percolation theory, combined with directly monitoring dynamic changes in tissue rheology and cell contact mechanics, we demonstrate that the zebrafish blastoderm undergoes a genuine rigidity PT, brought about by a small reduction in adhesion-dependent cell connectivity below a critical value. We quantitatively predict and experimentally verify hallmarks of PTs, including power-law exponents and associated discontinuities of macroscopic observables. Finally, we show that this uniform PT depends on blastoderm cells undergoing meta-synchronous divisions causing random and, consequently, uniform changes in cell connectivity. Collectively, our theoretical and experimental findings reveal the structural basis of material PTs in an organismal context.","lang":"eng"}],"scopus_import":"1","doi":"10.1016/j.cell.2021.02.017","day":"01","date_published":"2021-04-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"1914-1928.e19","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"language":[{"iso":"eng"}],"oa":1,"related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/embryonic-tissue-undergoes-phase-transition/"}]},"article_processing_charge":"No","has_accepted_license":"1","file_date_updated":"2021-06-08T10:04:10Z","corr_author":"1","ec_funded":1,"ddc":["570"],"type":"journal_article","status":"public","isi":1,"fulldoi":"https://doi.org/10.1016/j.cell.2021.02.017","file":[{"creator":"cziletti","checksum":"1e5295fbd9c2a459173ec45a0e8a7c2e","file_id":"9534","file_size":11405875,"date_created":"2021-06-08T10:04:10Z","content_type":"application/pdf","file_name":"2021_Cell_Petridou.pdf","access_level":"open_access","relation":"main_file","success":1,"date_updated":"2021-06-08T10:04:10Z"}],"project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","call_identifier":"H2020"},{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020"},{"call_identifier":"FWF","name":"Tissue material properties in embryonic development","_id":"2693FD8C-B435-11E9-9278-68D0E5697425","grant_number":"V00736"}],"title":"Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions","publication_status":"published"},{"corr_author":"1","article_processing_charge":"No","oa":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","page":"733–744","language":[{"iso":"eng"}],"title":"Cell fate coordinates mechano-osmotic forces in intestinal crypt formation","project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis"},{"name":"Active mechano-chemical description of the cell cytoskeleton","_id":"268294B6-B435-11E9-9278-68D0E5697425","grant_number":"P31639","call_identifier":"FWF"}],"publication_status":"published","fulldoi":"https://doi.org/10.1038/s41556-021-00700-2","isi":1,"status":"public","type":"journal_article","ec_funded":1,"pmid":1,"quality_controlled":"1","volume":23,"article_type":"original","publication_identifier":{"issn":["1465-7392"],"eissn":["1476-4679"]},"intvolume":"        23","external_id":{"isi":["000664016300003"],"pmid":["34155381"]},"oa_version":"Preprint","month":"06","publisher":"Springer Nature","_id":"9629","department":[{"_id":"EdHa"}],"acknowledgement":"We acknowledge the members of the Lennon-Duménil laboratory for sharing the mouse line of Myh9-GFP. We are grateful to the members of the Liberali laboratory and the FMI facilities for their support. We thank E. Tagliavini for IT support; L. Gelman for assistance and training; S. Bichet and A. Bogucki for helping with histology of mouse tissues; H. Kohler for fluorescence-activated cell sorting; G. Q. G. de Medeiros for maintenance of light-sheet microscopy; M. G. Stadler for scRNA-seq analysis; G. Gay for discussions on the 3D vertex model; the members of the Liberali laboratory, C. P. Heisenberg and C. Tsiairis for reading and providing feedback on the manuscript. Funding: Q.Y. is supported by a Postdoc fellowship from Peter und Taul Engelhorn Stiftung (PTES). This work received funding from the European Research Council (ERC) under the EU Horizon 2020 research and Innovation Programme Grant Agreement no. 758617 (to P.L.), the Swiss National Foundation (SNF) (POOP3_157531, to P.L.) and from the ERC under the EU Horizon 2020 Research and Innovation Program Grant Agreements 851288 (to E.H.) and the Austrian Science Fund (FWF) (P31639, to E.H.).","doi":"10.1038/s41556-021-00700-2","date_published":"2021-06-21T00:00:00Z","day":"21","scopus_import":"1","abstract":[{"lang":"eng","text":"Intestinal organoids derived from single cells undergo complex crypt–villus patterning and morphogenesis. However, the nature and coordination of the underlying forces remains poorly characterized. Here, using light-sheet microscopy and large-scale imaging quantification, we demonstrate that crypt formation coincides with a stark reduction in lumen volume. We develop a 3D biophysical model to computationally screen different mechanical scenarios of crypt morphogenesis. Combining this with live-imaging data and multiple mechanical perturbations, we show that actomyosin-driven crypt apical contraction and villus basal tension work synergistically with lumen volume reduction to drive crypt morphogenesis, and demonstrate the existence of a critical point in differential tensions above which crypt morphology becomes robust to volume changes. Finally, we identified a sodium/glucose cotransporter that is specific to differentiated enterocytes that modulates lumen volume reduction through cell swelling in the villus region. Together, our study uncovers the cellular basis of how cell fate modulates osmotic and actomyosin forces to coordinate robust morphogenesis."}],"date_updated":"2025-04-14T07:52:26Z","author":[{"last_name":"Yang","first_name":"Qiutan","full_name":"Yang, Qiutan"},{"full_name":"Xue, Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","first_name":"Shi-lei","last_name":"Xue"},{"full_name":"Chan, Chii Jou","last_name":"Chan","first_name":"Chii Jou"},{"full_name":"Rempfler, Markus","first_name":"Markus","last_name":"Rempfler"},{"full_name":"Vischi, Dario","first_name":"Dario","last_name":"Vischi"},{"last_name":"Maurer-Gutierrez","first_name":"Francisca","full_name":"Maurer-Gutierrez, Francisca"},{"full_name":"Hiiragi, Takashi","first_name":"Takashi","last_name":"Hiiragi"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Prisca","last_name":"Liberali","full_name":"Liberali, Prisca"}],"publication":"Nature Cell Biology","main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/2020.05.13.094359"}],"date_created":"2021-07-04T22:01:25Z","year":"2021","citation":{"apa":"Yang, Q., Xue, S., Chan, C. J., Rempfler, M., Vischi, D., Maurer-Gutierrez, F., … Liberali, P. (2021). Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41556-021-00700-2\">https://doi.org/10.1038/s41556-021-00700-2</a>","ama":"Yang Q, Xue S, Chan CJ, et al. Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. <i>Nature Cell Biology</i>. 2021;23:733–744. doi:<a href=\"https://doi.org/10.1038/s41556-021-00700-2\">10.1038/s41556-021-00700-2</a>","ista":"Yang Q, Xue S, Chan CJ, Rempfler M, Vischi D, Maurer-Gutierrez F, Hiiragi T, Hannezo EB, Liberali P. 2021. Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. Nature Cell Biology. 23, 733–744.","short":"Q. Yang, S. Xue, C.J. Chan, M. Rempfler, D. Vischi, F. Maurer-Gutierrez, T. Hiiragi, E.B. Hannezo, P. Liberali, Nature Cell Biology 23 (2021) 733–744.","ieee":"Q. Yang <i>et al.</i>, “Cell fate coordinates mechano-osmotic forces in intestinal crypt formation,” <i>Nature Cell Biology</i>, vol. 23. Springer Nature, pp. 733–744, 2021.","mla":"Yang, Qiutan, et al. “Cell Fate Coordinates Mechano-Osmotic Forces in Intestinal Crypt Formation.” <i>Nature Cell Biology</i>, vol. 23, Springer Nature, 2021, pp. 733–744, doi:<a href=\"https://doi.org/10.1038/s41556-021-00700-2\">10.1038/s41556-021-00700-2</a>.","chicago":"Yang, Qiutan, Shi-lei Xue, Chii Jou Chan, Markus Rempfler, Dario Vischi, Francisca Maurer-Gutierrez, Takashi Hiiragi, Edouard B Hannezo, and Prisca Liberali. “Cell Fate Coordinates Mechano-Osmotic Forces in Intestinal Crypt Formation.” <i>Nature Cell Biology</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41556-021-00700-2\">https://doi.org/10.1038/s41556-021-00700-2</a>."}},{"article_number":"jcs255018","issue":"14","quality_controlled":"1","volume":134,"article_type":"original","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"intvolume":"       134","external_id":{"isi":["000681395800008"]},"month":"07","oa_version":"Published Version","publisher":"The Company of Biologists","_id":"9952","department":[{"_id":"EdHa"}],"acknowledgement":"We would like to thank the entire Paluch and Baum laboratories at the MRC-LMCB and the Chalut lab at the Cambridge SCI for discussions and feedback throughout the project, and the MRC-LMCB microscopy platform, in particular Andrew Vaughan, for technical support.","doi":"10.1242/jcs.255018","date_published":"2021-07-01T00:00:00Z","day":"01","abstract":[{"lang":"eng","text":"Proper control of division orientation and symmetry, largely determined by spindle positioning, is essential to development and homeostasis. Spindle positioning has been extensively studied in cells dividing in two-dimensional (2D) environments and in epithelial tissues, where proteins such as NuMA (also known as NUMA1) orient division along the interphase long axis of the cell. However, little is known about how cells control spindle positioning in three-dimensional (3D) environments, such as early mammalian embryos and a variety of adult tissues. Here, we use mouse embryonic stem cells (ESCs), which grow in 3D colonies, as a model to investigate division in 3D. We observe that, at the periphery of 3D colonies, ESCs display high spindle mobility and divide asymmetrically. Our data suggest that enhanced spindle movements are due to unequal distribution of the cell–cell junction protein E-cadherin between future daughter cells. Interestingly, when cells progress towards differentiation, division becomes more symmetric, with more elongated shapes in metaphase and enhanced cortical NuMA recruitment in anaphase. Altogether, this study suggests that in 3D contexts, the geometry of the cell and its contacts with neighbors control division orientation and symmetry."}],"scopus_import":"1","date_updated":"2025-07-10T12:02:07Z","author":[{"last_name":"Chaigne","first_name":"Agathe","full_name":"Chaigne, Agathe"},{"first_name":"Matthew B.","last_name":"Smith","full_name":"Smith, Matthew B."},{"full_name":"Cavestany, R. L.","last_name":"Cavestany","first_name":"R. L."},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Chalut","first_name":"Kevin J.","full_name":"Chalut, Kevin J."},{"full_name":"Paluch, Ewa K.","last_name":"Paluch","first_name":"Ewa K."}],"publication":"Journal of Cell Science","year":"2021","date_created":"2021-08-22T22:01:20Z","citation":{"ieee":"A. Chaigne, M. B. Smith, R. L. Cavestany, E. B. Hannezo, K. J. Chalut, and E. K. Paluch, “Three-dimensional geometry controls division symmetry in stem cell colonies,” <i>Journal of Cell Science</i>, vol. 134, no. 14. The Company of Biologists, 2021.","short":"A. Chaigne, M.B. Smith, R.L. Cavestany, E.B. Hannezo, K.J. Chalut, E.K. Paluch, Journal of Cell Science 134 (2021).","mla":"Chaigne, Agathe, et al. “Three-Dimensional Geometry Controls Division Symmetry in Stem Cell Colonies.” <i>Journal of Cell Science</i>, vol. 134, no. 14, jcs255018, The Company of Biologists, 2021, doi:<a href=\"https://doi.org/10.1242/jcs.255018\">10.1242/jcs.255018</a>.","chicago":"Chaigne, Agathe, Matthew B. Smith, R. L. Cavestany, Edouard B Hannezo, Kevin J. Chalut, and Ewa K. Paluch. “Three-Dimensional Geometry Controls Division Symmetry in Stem Cell Colonies.” <i>Journal of Cell Science</i>. The Company of Biologists, 2021. <a href=\"https://doi.org/10.1242/jcs.255018\">https://doi.org/10.1242/jcs.255018</a>.","apa":"Chaigne, A., Smith, M. B., Cavestany, R. L., Hannezo, E. B., Chalut, K. J., &#38; Paluch, E. K. (2021). Three-dimensional geometry controls division symmetry in stem cell colonies. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.255018\">https://doi.org/10.1242/jcs.255018</a>","ista":"Chaigne A, Smith MB, Cavestany RL, Hannezo EB, Chalut KJ, Paluch EK. 2021. Three-dimensional geometry controls division symmetry in stem cell colonies. Journal of Cell Science. 134(14), jcs255018.","ama":"Chaigne A, Smith MB, Cavestany RL, Hannezo EB, Chalut KJ, Paluch EK. Three-dimensional geometry controls division symmetry in stem cell colonies. <i>Journal of Cell Science</i>. 2021;134(14). doi:<a href=\"https://doi.org/10.1242/jcs.255018\">10.1242/jcs.255018</a>"},"file_date_updated":"2021-08-23T07:32:20Z","has_accepted_license":"1","article_processing_charge":"Yes (in subscription journal)","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"language":[{"iso":"eng"}],"file":[{"file_id":"9954","file_size":8651724,"checksum":"f086f9d7cb63b2474c01921cb060c513","creator":"asandaue","date_updated":"2021-08-23T07:32:20Z","success":1,"relation":"main_file","file_name":"2021_JournalOfCellScience_Chaigne.pdf","access_level":"open_access","date_created":"2021-08-23T07:32:20Z","content_type":"application/pdf"}],"title":"Three-dimensional geometry controls division symmetry in stem cell colonies","publication_status":"published","fulldoi":"https://doi.org/10.1242/jcs.255018","isi":1,"status":"public","type":"journal_article","ddc":["570"]},{"type":"journal_article","ec_funded":1,"status":"public","fulldoi":"https://doi.org/10.1038/s41567-020-01037-7","isi":1,"title":"Theory of mechanochemical patterning and optimal migration in cell monolayers","project":[{"call_identifier":"FWF","name":"Active mechano-chemical description of the cell cytoskeleton","_id":"268294B6-B435-11E9-9278-68D0E5697425","grant_number":"P31639"},{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020"},{"call_identifier":"H2020","name":"International IST Doctoral Program","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"}],"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"267-274","language":[{"iso":"eng"}],"article_processing_charge":"No","oa":1,"related_material":{"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/wound-healing-waves/","relation":"press_release"}],"record":[{"relation":"dissertation_contains","status":"public","id":"12964"}]},"corr_author":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2020.05.15.096479"}],"publication":"Nature Physics","date_created":"2020-10-04T22:01:37Z","year":"2021","citation":{"chicago":"Boocock, Daniel R, Naoya Hino, Natalia Ruzickova, Tsuyoshi Hirashima, and Edouard B Hannezo. “Theory of Mechanochemical Patterning and Optimal Migration in Cell Monolayers.” <i>Nature Physics</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41567-020-01037-7\">https://doi.org/10.1038/s41567-020-01037-7</a>.","short":"D.R. Boocock, N. Hino, N. Ruzickova, T. Hirashima, E.B. Hannezo, Nature Physics 17 (2021) 267–274.","ieee":"D. R. Boocock, N. Hino, N. Ruzickova, T. Hirashima, and E. B. Hannezo, “Theory of mechanochemical patterning and optimal migration in cell monolayers,” <i>Nature Physics</i>, vol. 17. Springer Nature, pp. 267–274, 2021.","mla":"Boocock, Daniel R., et al. “Theory of Mechanochemical Patterning and Optimal Migration in Cell Monolayers.” <i>Nature Physics</i>, vol. 17, Springer Nature, 2021, pp. 267–74, doi:<a href=\"https://doi.org/10.1038/s41567-020-01037-7\">10.1038/s41567-020-01037-7</a>.","ama":"Boocock DR, Hino N, Ruzickova N, Hirashima T, Hannezo EB. Theory of mechanochemical patterning and optimal migration in cell monolayers. <i>Nature Physics</i>. 2021;17:267-274. doi:<a href=\"https://doi.org/10.1038/s41567-020-01037-7\">10.1038/s41567-020-01037-7</a>","ista":"Boocock DR, Hino N, Ruzickova N, Hirashima T, Hannezo EB. 2021. Theory of mechanochemical patterning and optimal migration in cell monolayers. Nature Physics. 17, 267–274.","apa":"Boocock, D. R., Hino, N., Ruzickova, N., Hirashima, T., &#38; Hannezo, E. B. (2021). Theory of mechanochemical patterning and optimal migration in cell monolayers. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-020-01037-7\">https://doi.org/10.1038/s41567-020-01037-7</a>"},"date_updated":"2026-10-20T22:30:04Z","author":[{"full_name":"Boocock, Daniel R","orcid":"0000-0002-1585-2631","first_name":"Daniel R","id":"453AF628-F248-11E8-B48F-1D18A9856A87","last_name":"Boocock"},{"full_name":"Hino, Naoya","last_name":"Hino","first_name":"Naoya"},{"id":"D2761128-D73D-11E9-A1BF-BA0DE6697425","first_name":"Natalia","last_name":"Ruzickova","full_name":"Ruzickova, Natalia"},{"first_name":"Tsuyoshi","last_name":"Hirashima","full_name":"Hirashima, Tsuyoshi"},{"last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"}],"doi":"10.1038/s41567-020-01037-7","day":"01","date_published":"2021-02-01T00:00:00Z","scopus_import":"1","abstract":[{"lang":"eng","text":"Collective cell migration offers a rich field of study for non-equilibrium physics and cellular biology, revealing phenomena such as glassy dynamics, pattern formation and active turbulence. However, how mechanical and chemical signalling are integrated at the cellular level to give rise to such collective behaviours remains unclear. We address this by focusing on the highly conserved phenomenon of spatiotemporal waves of density and extracellular signal-regulated kinase (ERK) activation, which appear both in vitro and in vivo during collective cell migration and wound healing. First, we propose a biophysical theory, backed by mechanical and optogenetic perturbation experiments, showing that patterns can be quantitatively explained by a mechanochemical coupling between active cellular tensions and the mechanosensitive ERK pathway. Next, we demonstrate how this biophysical mechanism can robustly induce long-ranged order and migration in a desired orientation, and we determine the theoretically optimal wavelength and period for inducing maximal migration towards free edges, which fits well with experimentally observed dynamics. We thereby provide a bridge between the biophysical origin of spatiotemporal instabilities and the design principles of robust and efficient long-ranged migration."}],"_id":"8602","department":[{"_id":"EdHa"}],"acknowledgement":"We would like to thank G. Tkacik and all of the members of the Hannezo and Hirashima groups for useful discussions, X. Trepat for help on traction force microscopy and M. Matsuda for use of the lab facility. E.H. acknowledges grants from the Austrian Science Fund (FWF) (P 31639) and the European Research Council (851288). T.H. acknowledges a grant from JST, PRESTO (JPMJPR1949). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 665385 (to D.B.), from JSPS KAKENHI grant no. 17J02107 (to N.H.) and from the SPIRITS 2018 of Kyoto University (to E.H. and T.H.).","external_id":{"isi":["000573519500002"]},"month":"02","oa_version":"Preprint","publisher":"Springer Nature","volume":17,"publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"article_type":"original","intvolume":"        17","quality_controlled":"1"},{"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"file_date_updated":"2021-04-27T08:38:35Z","article_processing_charge":"No","has_accepted_license":"1","oa":1,"related_material":{"record":[{"relation":"dissertation_contains","id":"13081","status":"public"}]},"fulldoi":"https://doi.org/10.1088/1478-3975/abd0db","isi":1,"file":[{"creator":"cziletti","checksum":"4f52082549d3561c4c15d4d8d84ca5d8","file_id":"9355","file_size":6296324,"content_type":"application/pdf","date_created":"2021-04-27T08:38:35Z","file_name":"2021_PhysBio_Lenne.pdf","access_level":"open_access","date_updated":"2021-04-27T08:38:35Z","relation":"main_file","success":1}],"title":"Roadmap for the multiscale coupling of biochemical and mechanical signals during development","project":[{"call_identifier":"H2020","_id":"B6FC0238-B512-11E9-945C-1524E6697425","grant_number":"680037","name":"Coordination of Patterning And Growth In the Spinal Cord"},{"call_identifier":"FWF","name":"Active mechano-chemical description of the cell cytoskeleton","_id":"268294B6-B435-11E9-9278-68D0E5697425","grant_number":"P31639"},{"call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E"}],"publication_status":"published","type":"journal_article","ec_funded":1,"ddc":["570"],"status":"public","volume":18,"publication_identifier":{"eissn":["1478-3975"]},"article_type":"original","intvolume":"        18","issue":"4","pmid":1,"article_number":"041501","quality_controlled":"1","_id":"9349","department":[{"_id":"AnKi"},{"_id":"EdHa"}],"acknowledgement":"The AK group is supported by IST Austria and by the ERC under European Union Horizon 2020 research and innovation programme Grant 680037. Apologies to those whose work could not be mentioned due to limited space. We thank all my lab members, both past and present, for stimulating discussion. This work was funded by a Singapore Ministry of Education Tier 3 Grant, MOE2016-T3-1-005. We thank Francis Corson for continuous discussion and collaboration contributing to these views and for figure 4(A). PC is sponsored by the Institut Pasteur and the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Grant Agreement No. 665807. Research in JG's laboratory is funded by the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement No. 337635, Institut Pasteur, CNRS, Cercle FSER, Fondation pour la Recherche Medicale, the Vallee Foundation and the ANR-19-CE-13-0024 Grant. We thank Erez Braun and Alex Mogilner for comments on the manuscript and Niv Ierushalmi for help with figure 5. This project has received funding from the European Union's Horizon 2020 research and innovation programme under Grant Agreement No. ERC-2018-COG Grant 819174-HydraMechanics awarded to KK. EH thanks all lab members, as well as Pierre Recho, Tsuyoshi Hirashima, Diana Pinheiro and Carl-Philip Heisenberg, for fruitful discussions on these topics—and apologize for not being able to cite many very relevant publications due to the strict 10-reference limit. EH acknowledges the support of Austrian Science Fund (FWF) (P 31639) and the European Research Council under the European Union's Horizon 2020 Research and Innovation Programme Grant Agreements (851288). The authors acknowledge the inspiring scientists whose work could not be cited in this perspective due to space constraints; the members of the Gartner Lab for helpful discussions; the Barbara and Gerson Bakar Foundation, the Chan Zuckerberg Biohub Investigators Programme, the National Institute of Health, and the Centre for Cellular Construction, an NSF Science and Technology Centre. The Minc laboratory is currently funded by the CNRS and the European Research Council (CoG Forcaster No. 647073). Research in the lab of J-LM is supported by the Institut Curie, the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé Et de la Recherche Médicale (INSERM), and is funded by grants from the ATIP-Avenir programme, the Fondation Schlumberger pour l'Éducation et la Recherche via the Fondation pour la Recherche Médicale, the European Research Council Starting Grant ERC-2017-StG 757557, the European Molecular Biology Organization Young Investigator programme (EMBO YIP), the INSERM transversal programme Human Development Cell Atlas (HuDeCA), Paris Sciences Lettres (PSL) 'nouvelle équipe' and QLife (17-CONV-0005) grants and Labex DEEP (ANR-11-LABX-0044) which are part of the IDEX PSL (ANR-10-IDEX-0001-02). We acknowledge useful discussions with Massimo Vergassola, Sebastian Streichan and my lab members. Work in my laboratory on Drosophila embryogenesis is partly supported by NIH-R01GM122936. The authors acknowledge the support by a grant from the European Research Council (Grant No. 682161). Lenne group is funded by a grant from the 'Investissements d'Avenir' French Government programme managed by the French National Research Agency (ANR-16-CONV-0001) and by the Excellence Initiative of Aix-Marseille University—A*MIDEX, and ANR projects MechaResp (ANR-17-CE13-0032) and AdGastrulo (ANR-19-CE13-0022).","external_id":{"isi":["000640396400001"],"pmid":["33276350"]},"month":"04","oa_version":"Published Version","publisher":"IOP Publishing","doi":"10.1088/1478-3975/abd0db","day":"14","date_published":"2021-04-14T00:00:00Z","scopus_import":"1","abstract":[{"text":"The way in which interactions between mechanics and biochemistry lead to the emergence of complex cell and tissue organization is an old question that has recently attracted renewed interest from biologists, physicists, mathematicians and computer scientists. Rapid advances in optical physics, microscopy and computational image analysis have greatly enhanced our ability to observe and quantify spatiotemporal patterns of signalling, force generation, deformation, and flow in living cells and tissues. Powerful new tools for genetic, biophysical and optogenetic manipulation are allowing us to perturb the underlying machinery that generates these patterns in increasingly sophisticated ways. Rapid advances in theory and computing have made it possible to construct predictive models that describe how cell and tissue organization and dynamics emerge from the local coupling of biochemistry and mechanics. Together, these advances have opened up a wealth of new opportunities to explore how mechanochemical patterning shapes organismal development. In this roadmap, we present a series of forward-looking case studies on mechanochemical patterning in development, written by scientists working at the interface between the physical and biological sciences, and covering a wide range of spatial and temporal scales, organisms, and modes of development. Together, these contributions highlight the many ways in which the dynamic coupling of mechanics and biochemistry shapes biological dynamics: from mechanoenzymes that sense force to tune their activity and motor output, to collectives of cells in tissues that flow and redistribute biochemical signals during development.","lang":"eng"}],"publication":"Physical biology","date_created":"2021-04-25T22:01:29Z","year":"2021","citation":{"mla":"Lenne, Pierre François, et al. “Roadmap for the Multiscale Coupling of Biochemical and Mechanical Signals during Development.” <i>Physical Biology</i>, vol. 18, no. 4, 041501, IOP Publishing, 2021, doi:<a href=\"https://doi.org/10.1088/1478-3975/abd0db\">10.1088/1478-3975/abd0db</a>.","ieee":"P. F. Lenne <i>et al.</i>, “Roadmap for the multiscale coupling of biochemical and mechanical signals during development,” <i>Physical biology</i>, vol. 18, no. 4. IOP Publishing, 2021.","short":"P.F. Lenne, E. Munro, I. Heemskerk, A. Warmflash, L. Bocanegra, K. Kishi, A. Kicheva, Y. Long, A. Fruleux, A. Boudaoud, T.E. Saunders, P. Caldarelli, A. Michaut, J. Gros, Y. Maroudas-Sacks, K. Keren, E.B. Hannezo, Z.J. Gartner, B. Stormo, A. Gladfelter, A. Rodrigues, A. Shyer, N. Minc, J.L. Maître, S. Di Talia, B. Khamaisi, D. Sprinzak, S. Tlili, Physical Biology 18 (2021).","chicago":"Lenne, Pierre François, Edwin Munro, Idse Heemskerk, Aryeh Warmflash, Laura Bocanegra, Kasumi Kishi, Anna Kicheva, et al. “Roadmap for the Multiscale Coupling of Biochemical and Mechanical Signals during Development.” <i>Physical Biology</i>. IOP Publishing, 2021. <a href=\"https://doi.org/10.1088/1478-3975/abd0db\">https://doi.org/10.1088/1478-3975/abd0db</a>.","apa":"Lenne, P. F., Munro, E., Heemskerk, I., Warmflash, A., Bocanegra, L., Kishi, K., … Tlili, S. (2021). Roadmap for the multiscale coupling of biochemical and mechanical signals during development. <i>Physical Biology</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/1478-3975/abd0db\">https://doi.org/10.1088/1478-3975/abd0db</a>","ista":"Lenne PF, Munro E, Heemskerk I, Warmflash A, Bocanegra L, Kishi K, Kicheva A, Long Y, Fruleux A, Boudaoud A, Saunders TE, Caldarelli P, Michaut A, Gros J, Maroudas-Sacks Y, Keren K, Hannezo EB, Gartner ZJ, Stormo B, Gladfelter A, Rodrigues A, Shyer A, Minc N, Maître JL, Di Talia S, Khamaisi B, Sprinzak D, Tlili S. 2021. Roadmap for the multiscale coupling of biochemical and mechanical signals during development. Physical biology. 18(4), 041501.","ama":"Lenne PF, Munro E, Heemskerk I, et al. Roadmap for the multiscale coupling of biochemical and mechanical signals during development. <i>Physical biology</i>. 2021;18(4). doi:<a href=\"https://doi.org/10.1088/1478-3975/abd0db\">10.1088/1478-3975/abd0db</a>"},"date_updated":"2026-10-20T22:30:20Z","author":[{"first_name":"Pierre François","last_name":"Lenne","full_name":"Lenne, Pierre François"},{"full_name":"Munro, Edwin","first_name":"Edwin","last_name":"Munro"},{"full_name":"Heemskerk, Idse","first_name":"Idse","last_name":"Heemskerk"},{"full_name":"Warmflash, Aryeh","first_name":"Aryeh","last_name":"Warmflash"},{"full_name":"Bocanegra, Laura","last_name":"Bocanegra","first_name":"Laura","id":"4896F754-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kishi","id":"3065DFC4-F248-11E8-B48F-1D18A9856A87","first_name":"Kasumi","orcid":"0000-0001-6060-4795","full_name":"Kishi, Kasumi"},{"last_name":"Kicheva","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","first_name":"Anna","orcid":"0000-0003-4509-4998","full_name":"Kicheva, Anna"},{"full_name":"Long, Yuchen","first_name":"Yuchen","last_name":"Long"},{"full_name":"Fruleux, Antoine","last_name":"Fruleux","first_name":"Antoine"},{"first_name":"Arezki","last_name":"Boudaoud","full_name":"Boudaoud, Arezki"},{"last_name":"Saunders","first_name":"Timothy E.","full_name":"Saunders, Timothy E."},{"full_name":"Caldarelli, Paolo","first_name":"Paolo","last_name":"Caldarelli"},{"full_name":"Michaut, Arthur","last_name":"Michaut","first_name":"Arthur"},{"full_name":"Gros, Jerome","first_name":"Jerome","last_name":"Gros"},{"full_name":"Maroudas-Sacks, Yonit","last_name":"Maroudas-Sacks","first_name":"Yonit"},{"full_name":"Keren, Kinneret","last_name":"Keren","first_name":"Kinneret"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"full_name":"Gartner, Zev J.","first_name":"Zev J.","last_name":"Gartner"},{"first_name":"Benjamin","last_name":"Stormo","full_name":"Stormo, Benjamin"},{"first_name":"Amy","last_name":"Gladfelter","full_name":"Gladfelter, Amy"},{"full_name":"Rodrigues, Alan","last_name":"Rodrigues","first_name":"Alan"},{"last_name":"Shyer","first_name":"Amy","full_name":"Shyer, Amy"},{"last_name":"Minc","first_name":"Nicolas","full_name":"Minc, Nicolas"},{"last_name":"Maître","first_name":"Jean Léon","full_name":"Maître, Jean Léon"},{"full_name":"Di Talia, Stefano","last_name":"Di Talia","first_name":"Stefano"},{"last_name":"Khamaisi","first_name":"Bassma","full_name":"Khamaisi, Bassma"},{"full_name":"Sprinzak, David","first_name":"David","last_name":"Sprinzak"},{"first_name":"Sham","last_name":"Tlili","full_name":"Tlili, Sham"}]},{"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","oa":1,"file_date_updated":"2020-07-14T12:48:03Z","has_accepted_license":"1","article_processing_charge":"No","language":[{"iso":"eng"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"page":"604-620.e22","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","file":[{"file_size":17992888,"file_id":"7795","creator":"dernst","checksum":"e2114902f4e9d75a752e9efb5ae06011","date_updated":"2020-07-14T12:48:03Z","relation":"main_file","content_type":"application/pdf","date_created":"2020-05-04T10:20:55Z","access_level":"open_access","file_name":"2020_Cell_Dekoninck.pdf"}],"title":"Defining the design principles of skin epidermis postnatal growth","isi":1,"fulldoi":"https://doi.org/10.1016/j.cell.2020.03.015","status":"public","ddc":["570"],"type":"journal_article","quality_controlled":"1","pmid":1,"issue":"3","intvolume":"       181","article_type":"original","publication_identifier":{"eissn":["1097-4172"],"issn":["0092-8674"]},"volume":181,"publisher":"Elsevier","month":"04","oa_version":"Published Version","external_id":{"pmid":["32259486"],"isi":["000530708400016"]},"department":[{"_id":"EdHa"}],"_id":"7789","scopus_import":"1","abstract":[{"text":"During embryonic and postnatal development, organs and tissues grow steadily to achieve their final size at the end of puberty. However, little is known about the cellular dynamics that mediate postnatal growth. By combining in vivo clonal lineage tracing, proliferation kinetics, single-cell transcriptomics, andin vitro micro-pattern experiments, we resolved the cellular dynamics taking place during postnatal skin epidermis expansion. Our data revealed that harmonious growth is engineered by a single population of developmental progenitors presenting a fixed fate imbalance of self-renewing divisions with an ever-decreasing proliferation rate. Single-cell RNA sequencing revealed that epidermal developmental progenitors form a more uniform population compared with adult stem and progenitor cells. Finally, we found that the spatial pattern of cell division orientation is dictated locally by the underlying collagen fiber orientation. Our results uncover a simple design principle of organ growth where progenitors and differentiated cells expand in harmony with their surrounding tissues.","lang":"eng"}],"day":"30","date_published":"2020-04-30T00:00:00Z","doi":"10.1016/j.cell.2020.03.015","author":[{"full_name":"Dekoninck, Sophie","first_name":"Sophie","last_name":"Dekoninck"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo"},{"first_name":"Alejandro","last_name":"Sifrim","full_name":"Sifrim, Alejandro"},{"full_name":"Miroshnikova, Yekaterina A.","last_name":"Miroshnikova","first_name":"Yekaterina A."},{"full_name":"Aragona, Mariaceleste","first_name":"Mariaceleste","last_name":"Aragona"},{"first_name":"Milan","last_name":"Malfait","full_name":"Malfait, Milan"},{"last_name":"Gargouri","first_name":"Souhir","full_name":"Gargouri, Souhir"},{"last_name":"De Neunheuser","first_name":"Charlotte","full_name":"De Neunheuser, Charlotte"},{"first_name":"Christine","last_name":"Dubois","full_name":"Dubois, Christine"},{"full_name":"Voet, Thierry","first_name":"Thierry","last_name":"Voet"},{"first_name":"Sara A.","last_name":"Wickström","full_name":"Wickström, Sara A."},{"full_name":"Simons, Benjamin D.","first_name":"Benjamin D.","last_name":"Simons"},{"last_name":"Blanpain","first_name":"Cédric","full_name":"Blanpain, Cédric"}],"date_updated":"2025-07-10T11:54:47Z","citation":{"mla":"Dekoninck, Sophie, et al. “Defining the Design Principles of Skin Epidermis Postnatal Growth.” <i>Cell</i>, vol. 181, no. 3, Elsevier, 2020, p. 604–620.e22, doi:<a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">10.1016/j.cell.2020.03.015</a>.","short":"S. Dekoninck, E.B. Hannezo, A. Sifrim, Y.A. Miroshnikova, M. Aragona, M. Malfait, S. Gargouri, C. De Neunheuser, C. Dubois, T. Voet, S.A. Wickström, B.D. Simons, C. Blanpain, Cell 181 (2020) 604–620.e22.","ieee":"S. Dekoninck <i>et al.</i>, “Defining the design principles of skin epidermis postnatal growth,” <i>Cell</i>, vol. 181, no. 3. Elsevier, p. 604–620.e22, 2020.","chicago":"Dekoninck, Sophie, Edouard B Hannezo, Alejandro Sifrim, Yekaterina A. Miroshnikova, Mariaceleste Aragona, Milan Malfait, Souhir Gargouri, et al. “Defining the Design Principles of Skin Epidermis Postnatal Growth.” <i>Cell</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">https://doi.org/10.1016/j.cell.2020.03.015</a>.","apa":"Dekoninck, S., Hannezo, E. B., Sifrim, A., Miroshnikova, Y. A., Aragona, M., Malfait, M., … Blanpain, C. (2020). Defining the design principles of skin epidermis postnatal growth. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">https://doi.org/10.1016/j.cell.2020.03.015</a>","ama":"Dekoninck S, Hannezo EB, Sifrim A, et al. Defining the design principles of skin epidermis postnatal growth. <i>Cell</i>. 2020;181(3):604-620.e22. doi:<a href=\"https://doi.org/10.1016/j.cell.2020.03.015\">10.1016/j.cell.2020.03.015</a>","ista":"Dekoninck S, Hannezo EB, Sifrim A, Miroshnikova YA, Aragona M, Malfait M, Gargouri S, De Neunheuser C, Dubois C, Voet T, Wickström SA, Simons BD, Blanpain C. 2020. Defining the design principles of skin epidermis postnatal growth. Cell. 181(3), 604–620.e22."},"year":"2020","date_created":"2020-05-03T22:00:48Z","publication":"Cell"},{"scopus_import":"1","abstract":[{"text":"Understanding to what extent stem cell potential is a cell-intrinsic property or an emergent behavior coming from global tissue dynamics and geometry is a key outstanding question of systems and stem cell biology. Here, we propose a theory of stem cell dynamics as a stochastic competition for access to a spatially localized niche, giving rise to a stochastic conveyor-belt model. Cell divisions produce a steady cellular stream which advects cells away from the niche, while random rearrangements enable cells away from the niche to be favorably repositioned. Importantly, even when assuming that all cells in a tissue are molecularly equivalent, we predict a common (“universal”) functional dependence of the long-term clonal survival probability on distance from the niche, as well as the emergence of a well-defined number of functional stem cells, dependent only on the rate of random movements vs. mitosis-driven advection. We test the predictions of this theory on datasets of pubertal mammary gland tips and embryonic kidney tips, as well as homeostatic intestinal crypts. Importantly, we find good agreement for the predicted functional dependency of the competition as a function of position, and thus functional stem cell number in each organ. This argues for a key role of positional fluctuations in dictating stem cell number and dynamics, and we discuss the applicability of this theory to other settings.","lang":"eng"}],"date_published":"2020-07-21T00:00:00Z","day":"21","doi":"10.1073/pnas.1921205117","citation":{"ama":"Corominas-Murtra B, Scheele CLGJ, Kishi K, et al. Stem cell lineage survival as a noisy competition for niche access. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2020;117(29):16969-16975. doi:<a href=\"https://doi.org/10.1073/pnas.1921205117\">10.1073/pnas.1921205117</a>","ista":"Corominas-Murtra B, Scheele CLGJ, Kishi K, Ellenbroek SIJ, Simons BD, Van Rheenen J, Hannezo EB. 2020. Stem cell lineage survival as a noisy competition for niche access. Proceedings of the National Academy of Sciences of the United States of America. 117(29), 16969–16975.","apa":"Corominas-Murtra, B., Scheele, C. L. G. J., Kishi, K., Ellenbroek, S. I. J., Simons, B. D., Van Rheenen, J., &#38; Hannezo, E. B. (2020). Stem cell lineage survival as a noisy competition for niche access. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1921205117\">https://doi.org/10.1073/pnas.1921205117</a>","chicago":"Corominas-Murtra, Bernat, Colinda L.G.J. Scheele, Kasumi Kishi, Saskia I.J. Ellenbroek, Benjamin D. Simons, Jacco Van Rheenen, and Edouard B Hannezo. “Stem Cell Lineage Survival as a Noisy Competition for Niche Access.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2020. <a href=\"https://doi.org/10.1073/pnas.1921205117\">https://doi.org/10.1073/pnas.1921205117</a>.","ieee":"B. Corominas-Murtra <i>et al.</i>, “Stem cell lineage survival as a noisy competition for niche access,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 117, no. 29. National Academy of Sciences, pp. 16969–16975, 2020.","short":"B. Corominas-Murtra, C.L.G.J. Scheele, K. Kishi, S.I.J. Ellenbroek, B.D. Simons, J. Van Rheenen, E.B. Hannezo, Proceedings of the National Academy of Sciences of the United States of America 117 (2020) 16969–16975.","mla":"Corominas-Murtra, Bernat, et al. “Stem Cell Lineage Survival as a Noisy Competition for Niche Access.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 117, no. 29, National Academy of Sciences, 2020, pp. 16969–75, doi:<a href=\"https://doi.org/10.1073/pnas.1921205117\">10.1073/pnas.1921205117</a>."},"date_created":"2020-08-09T22:00:52Z","year":"2020","publication":"Proceedings of the National Academy of Sciences of the United States of America","author":[{"first_name":"Bernat","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","last_name":"Corominas-Murtra","full_name":"Corominas-Murtra, Bernat","orcid":"0000-0001-9806-5643"},{"full_name":"Scheele, Colinda L.G.J.","last_name":"Scheele","first_name":"Colinda L.G.J."},{"id":"3065DFC4-F248-11E8-B48F-1D18A9856A87","first_name":"Kasumi","last_name":"Kishi","full_name":"Kishi, Kasumi","orcid":"0000-0001-6060-4795"},{"last_name":"Ellenbroek","first_name":"Saskia I.J.","full_name":"Ellenbroek, Saskia I.J."},{"first_name":"Benjamin D.","last_name":"Simons","full_name":"Simons, Benjamin D."},{"last_name":"Van Rheenen","first_name":"Jacco","full_name":"Van Rheenen, Jacco"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"}],"date_updated":"2026-04-03T09:29:04Z","intvolume":"       117","publication_identifier":{"eissn":["1091-6490"]},"article_type":"original","volume":117,"quality_controlled":"1","pmid":1,"issue":"29","acknowledgement":"We thank all members of the E.H., B.D.S., and J.v.R. groups for stimulating discussions. This project was supported by\r\nthe European Research Council (648804 to J.v.R. and 851288 to E.H.). It has also received support from the CancerGenomics.nl (Netherlands Organization for Scientific Research) program (J.v.R.) and the Doctor Josef Steiner Foundation (J.v.R). B.D.S. was supported by Royal Society E. P. Abraham Research Professorship RP/R1/180165 and Wellcome Trust Grant 098357/Z/12/Z.","department":[{"_id":"EdHa"}],"_id":"8220","publisher":"National Academy of Sciences","oa_version":"Published Version","month":"07","external_id":{"pmid":["32611816"],"isi":["000553292900014"]},"isi":1,"fulldoi":"https://doi.org/10.1073/pnas.1921205117","publication_status":"published","title":"Stem cell lineage survival as a noisy competition for niche access","file":[{"date_created":"2020-08-10T06:50:28Z","content_type":"application/pdf","access_level":"open_access","file_name":"2020_PNAS_Corominas.pdf","date_updated":"2020-08-10T06:50:28Z","relation":"main_file","success":1,"creator":"dernst","file_id":"8223","file_size":1111604}],"project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis"}],"ec_funded":1,"ddc":["570"],"type":"journal_article","status":"public","corr_author":"1","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"page":"16969-16975","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/order-from-noise/"}]},"oa":1,"file_date_updated":"2020-08-10T06:50:28Z","article_processing_charge":"No","has_accepted_license":"1"},{"department":[{"_id":"EdHa"}],"_id":"8669","external_id":{"isi":["000577244600003"],"pmid":["33028844"]},"publisher":"Springer Nature","month":"10","oa_version":"Published Version","article_type":"original","publication_identifier":{"eissn":["2041-1723"]},"volume":11,"intvolume":"        11","pmid":1,"article_number":"5037","quality_controlled":"1","publication":"Nature Communications","citation":{"apa":"Sznurkowska, M. K., Hannezo, E. B., Azzarelli, R., Chatzeli, L., Ikeda, T., Yoshida, S., … Simons, B. D. (2020). Tracing the cellular basis of islet specification in mouse pancreas. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-18837-3\">https://doi.org/10.1038/s41467-020-18837-3</a>","ama":"Sznurkowska MK, Hannezo EB, Azzarelli R, et al. Tracing the cellular basis of islet specification in mouse pancreas. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-18837-3\">10.1038/s41467-020-18837-3</a>","ista":"Sznurkowska MK, Hannezo EB, Azzarelli R, Chatzeli L, Ikeda T, Yoshida S, Philpott A, Simons BD. 2020. Tracing the cellular basis of islet specification in mouse pancreas. Nature Communications. 11, 5037.","mla":"Sznurkowska, Magdalena K., et al. “Tracing the Cellular Basis of Islet Specification in Mouse Pancreas.” <i>Nature Communications</i>, vol. 11, 5037, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-18837-3\">10.1038/s41467-020-18837-3</a>.","short":"M.K. Sznurkowska, E.B. Hannezo, R. Azzarelli, L. Chatzeli, T. Ikeda, S. Yoshida, A. Philpott, B.D. Simons, Nature Communications 11 (2020).","ieee":"M. K. Sznurkowska <i>et al.</i>, “Tracing the cellular basis of islet specification in mouse pancreas,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","chicago":"Sznurkowska, Magdalena K., Edouard B Hannezo, Roberta Azzarelli, Lemonia Chatzeli, Tatsuro Ikeda, Shosei Yoshida, Anna Philpott, and Benjamin D Simons. “Tracing the Cellular Basis of Islet Specification in Mouse Pancreas.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-18837-3\">https://doi.org/10.1038/s41467-020-18837-3</a>."},"date_created":"2020-10-18T22:01:35Z","year":"2020","date_updated":"2026-04-02T14:29:58Z","author":[{"full_name":"Sznurkowska, Magdalena K.","first_name":"Magdalena K.","last_name":"Sznurkowska"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"full_name":"Azzarelli, Roberta","first_name":"Roberta","last_name":"Azzarelli"},{"full_name":"Chatzeli, Lemonia","last_name":"Chatzeli","first_name":"Lemonia"},{"full_name":"Ikeda, Tatsuro","last_name":"Ikeda","first_name":"Tatsuro"},{"first_name":"Shosei","last_name":"Yoshida","full_name":"Yoshida, Shosei"},{"full_name":"Philpott, Anna","last_name":"Philpott","first_name":"Anna"},{"last_name":"Simons","first_name":"Benjamin D","full_name":"Simons, Benjamin D"}],"day":"07","date_published":"2020-10-07T00:00:00Z","doi":"10.1038/s41467-020-18837-3","abstract":[{"text":"Pancreatic islets play an essential role in regulating blood glucose level. Although the molecular pathways underlying islet cell differentiation are beginning to be resolved, the cellular basis of islet morphogenesis and fate allocation remain unclear. By combining unbiased and targeted lineage tracing, we address the events leading to islet formation in the mouse. From the statistical analysis of clones induced at multiple embryonic timepoints, here we show that, during the secondary transition, islet formation involves the aggregation of multiple equipotent endocrine progenitors that transition from a phase of stochastic amplification by cell division into a phase of sublineage restriction and limited islet fission. Together, these results explain quantitatively the heterogeneous size distribution and degree of polyclonality of maturing islets, as well as dispersion of progenitors within and between islets. Further, our results show that, during the secondary transition, α- and β-cells are generated in a contemporary manner. Together, these findings provide insight into the cellular basis of islet development.","lang":"eng"}],"scopus_import":"1","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_processing_charge":"No","has_accepted_license":"1","file_date_updated":"2020-10-19T11:27:46Z","oa":1,"type":"journal_article","ddc":["570"],"status":"public","fulldoi":"https://doi.org/10.1038/s41467-020-18837-3","isi":1,"publication_status":"published","file":[{"file_name":"2020_NatureComm_Sznurkowska.pdf","access_level":"open_access","content_type":"application/pdf","date_created":"2020-10-19T11:27:46Z","date_updated":"2020-10-19T11:27:46Z","relation":"main_file","success":1,"checksum":"0ecc0eab72d2d50694852579611a6624","creator":"dernst","file_id":"8677","file_size":5540540}],"title":"Tracing the cellular basis of islet specification in mouse pancreas"},{"status":"public","type":"journal_article","ddc":["570"],"file":[{"date_updated":"2021-02-04T10:20:02Z","success":1,"relation":"main_file","access_level":"open_access","file_name":"2020_DevelopmCell_Chaigne.pdf","content_type":"application/pdf","date_created":"2021-02-04T10:20:02Z","file_size":6929686,"file_id":"9086","checksum":"88e1a031a61689165d19a19c2f16d795","creator":"dernst"}],"title":"Abscission couples cell division to embryonic stem cell fate","publication_status":"published","fulldoi":"https://doi.org/10.1016/j.devcel.2020.09.001","isi":1,"file_date_updated":"2021-02-04T10:20:02Z","article_processing_charge":"No","has_accepted_license":"1","oa":1,"page":"195-208","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"date_updated":"2025-07-10T11:57:15Z","author":[{"full_name":"Chaigne, Agathe","first_name":"Agathe","last_name":"Chaigne"},{"last_name":"Labouesse","first_name":"Céline","full_name":"Labouesse, Céline"},{"full_name":"White, Ian J.","first_name":"Ian J.","last_name":"White"},{"last_name":"Agnew","first_name":"Meghan","full_name":"Agnew, Meghan"},{"last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"last_name":"Chalut","first_name":"Kevin J.","full_name":"Chalut, Kevin J."},{"full_name":"Paluch, Ewa K.","first_name":"Ewa K.","last_name":"Paluch"}],"publication":"Developmental Cell","date_created":"2020-10-18T22:01:37Z","year":"2020","citation":{"ama":"Chaigne A, Labouesse C, White IJ, et al. Abscission couples cell division to embryonic stem cell fate. <i>Developmental Cell</i>. 2020;55(2):195-208. doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">10.1016/j.devcel.2020.09.001</a>","ista":"Chaigne A, Labouesse C, White IJ, Agnew M, Hannezo EB, Chalut KJ, Paluch EK. 2020. Abscission couples cell division to embryonic stem cell fate. Developmental Cell. 55(2), 195–208.","apa":"Chaigne, A., Labouesse, C., White, I. J., Agnew, M., Hannezo, E. B., Chalut, K. J., &#38; Paluch, E. K. (2020). Abscission couples cell division to embryonic stem cell fate. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">https://doi.org/10.1016/j.devcel.2020.09.001</a>","chicago":"Chaigne, Agathe, Céline Labouesse, Ian J. White, Meghan Agnew, Edouard B Hannezo, Kevin J. Chalut, and Ewa K. Paluch. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” <i>Developmental Cell</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">https://doi.org/10.1016/j.devcel.2020.09.001</a>.","mla":"Chaigne, Agathe, et al. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” <i>Developmental Cell</i>, vol. 55, no. 2, Elsevier, 2020, pp. 195–208, doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">10.1016/j.devcel.2020.09.001</a>.","short":"A. Chaigne, C. Labouesse, I.J. White, M. Agnew, E.B. Hannezo, K.J. Chalut, E.K. Paluch, Developmental Cell 55 (2020) 195–208.","ieee":"A. Chaigne <i>et al.</i>, “Abscission couples cell division to embryonic stem cell fate,” <i>Developmental Cell</i>, vol. 55, no. 2. Elsevier, pp. 195–208, 2020."},"doi":"10.1016/j.devcel.2020.09.001","day":"26","date_published":"2020-10-26T00:00:00Z","scopus_import":"1","abstract":[{"lang":"eng","text":"Cell fate transitions are key to development and homeostasis. It is thus essential to understand the cellular mechanisms controlling fate transitions. Cell division has been implicated in fate decisions in many stem cell types, including neuronal and epithelial progenitors. In other stem cells, such as embryonic stem (ES) cells, the role of division remains unclear. Here, we show that exit from naive pluripotency in mouse ES cells generally occurs after a division. We further show that exit timing is strongly correlated between sister cells, which remain connected by cytoplasmic bridges long after division, and that bridge abscission progressively accelerates as cells exit naive pluripotency. Finally, interfering with abscission impairs naive pluripotency exit, and artificially inducing abscission accelerates it. Altogether, our data indicate that a switch in the division machinery leading to faster abscission regulates pluripotency exit. Our study identifies abscission as a key cellular process coupling cell division to fate transitions."}],"external_id":{"pmid":["32979313"],"isi":["000582501100012"]},"oa_version":"Published Version","month":"10","publisher":"Elsevier","_id":"8672","department":[{"_id":"EdHa"}],"acknowledgement":"This work was supported by the Medical Research Council UK (MRC Program award MC_UU_12018/5 ), the European Research Council (starting grant 311637 -MorphoCorDiv and consolidator grant 820188 -NanoMechShape to E.K.P.), and the Leverhulme Trust (Leverhulme Prize in Biological Sciences to E.K.P.). K.J.C. acknowledges support from the Royal Society (Royal Society Research Fellowship). A.C. acknowledges support from EMBO ( ALTF 2015-563 ), the Wellcome Trust ( 201334/Z/16/Z ), and the Fondation Bettencourt-Schueller (Prix Jeune Chercheur, 2015).","pmid":1,"issue":"2","quality_controlled":"1","volume":55,"article_type":"original","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"intvolume":"        55"},{"type":"journal_article","ddc":["570"],"status":"public","fulldoi":"https://doi.org/10.1021/acs.nanolett.9b04445","isi":1,"title":"Collective force generation by molecular motors is determined by strain-induced unbinding","publication_status":"published","page":"669-676","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"article_processing_charge":"No","oa":1,"related_material":{"record":[{"relation":"research_data","id":"9726","status":"public"},{"relation":"research_data","id":"9885","status":"public"}]},"corr_author":"1","main_file_link":[{"url":"https://doi.org/10.1021/acs.nanolett.9b04445","open_access":"1"}],"publication":"Nano Letters","date_created":"2019-12-10T15:36:05Z","year":"2020","citation":{"apa":"Ucar, M. C., &#38; Lipowsky, R. (2020). Collective force generation by molecular motors is determined by strain-induced unbinding. <i>Nano Letters</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445\">https://doi.org/10.1021/acs.nanolett.9b04445</a>","ista":"Ucar MC, Lipowsky R. 2020. Collective force generation by molecular motors is determined by strain-induced unbinding. Nano Letters. 20(1), 669–676.","ama":"Ucar MC, Lipowsky R. Collective force generation by molecular motors is determined by strain-induced unbinding. <i>Nano Letters</i>. 2020;20(1):669-676. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445\">10.1021/acs.nanolett.9b04445</a>","mla":"Ucar, Mehmet C., and Reinhard Lipowsky. “Collective Force Generation by Molecular Motors Is Determined by Strain-Induced Unbinding.” <i>Nano Letters</i>, vol. 20, no. 1, American Chemical Society, 2020, pp. 669–76, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445\">10.1021/acs.nanolett.9b04445</a>.","short":"M.C. Ucar, R. Lipowsky, Nano Letters 20 (2020) 669–676.","ieee":"M. C. Ucar and R. Lipowsky, “Collective force generation by molecular motors is determined by strain-induced unbinding,” <i>Nano Letters</i>, vol. 20, no. 1. American Chemical Society, pp. 669–676, 2020.","chicago":"Ucar, Mehmet C, and Reinhard Lipowsky. “Collective Force Generation by Molecular Motors Is Determined by Strain-Induced Unbinding.” <i>Nano Letters</i>. American Chemical Society, 2020. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445\">https://doi.org/10.1021/acs.nanolett.9b04445</a>."},"date_updated":"2026-07-06T12:14:33Z","author":[{"last_name":"Ucar","first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425","orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C"},{"full_name":"Lipowsky, Reinhard","first_name":"Reinhard","last_name":"Lipowsky"}],"doi":"10.1021/acs.nanolett.9b04445","date_published":"2020-01-08T00:00:00Z","day":"08","abstract":[{"text":"In the living cell, we encounter a large variety of motile processes such as organelle transport and cytoskeleton remodeling. These processes are driven by motor proteins that generate force by transducing chemical free energy into mechanical work. In many cases, the molecular motors work in teams to collectively generate larger forces. Recent optical trapping experiments on small teams of cytoskeletal motors indicated that the collectively generated force increases with the size of the motor team but that this increase depends on the motor type and on whether the motors are studied in vitro or in vivo. Here, we use the theory of stochastic processes to describe the motion of N motors in a stationary optical trap and to compute the N-dependence of the collectively generated forces. We consider six distinct motor types, two kinesins, two dyneins, and two myosins. We show that the force increases always linearly with N but with a prefactor that depends on the performance of the single motor. Surprisingly, this prefactor increases for weaker motors with a lower stall force. This counter-intuitive behavior reflects the increased probability with which stronger motors detach from the filament during strain generation. Our theoretical results are in quantitative agreement with experimental data on small teams of kinesin-1 motors.","lang":"eng"}],"scopus_import":"1","_id":"7166","department":[{"_id":"EdHa"}],"external_id":{"isi":["000507151600087"],"pmid":["31797672"]},"month":"01","oa_version":"Published Version","publisher":"American Chemical Society","volume":20,"article_type":"letter_note","publication_identifier":{"issn":["1530-6984"],"eissn":["1530-6992"]},"intvolume":"        20","issue":"1","pmid":1,"quality_controlled":"1"},{"_id":"9885","department":[{"_id":"EdHa"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","oa_version":"Published Version","month":"01","related_material":{"record":[{"status":"public","id":"7166","relation":"used_in_publication"}]},"publisher":"American Chemical Society","type":"research_data_reference","year":"2020","date_created":"2021-08-11T13:16:03Z","citation":{"ista":"Ucar MC, Lipowsky R. 2020. MURL_Dataz, American Chemical Society, <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">10.1021/acs.nanolett.9b04445.s002</a>.","ama":"Ucar MC, Lipowsky R. MURL_Dataz. 2020. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">10.1021/acs.nanolett.9b04445.s002</a>","apa":"Ucar, M. C., &#38; Lipowsky, R. (2020). MURL_Dataz. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">https://doi.org/10.1021/acs.nanolett.9b04445.s002</a>","chicago":"Ucar, Mehmet C, and Reinhard Lipowsky. “MURL_Dataz.” American Chemical Society, 2020. <a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">https://doi.org/10.1021/acs.nanolett.9b04445.s002</a>.","short":"M.C. Ucar, R. Lipowsky, (2020).","ieee":"M. C. Ucar and R. Lipowsky, “MURL_Dataz.” American Chemical Society, 2020.","mla":"Ucar, Mehmet C., and Reinhard Lipowsky. <i>MURL_Dataz</i>. American Chemical Society, 2020, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.9b04445.s002\">10.1021/acs.nanolett.9b04445.s002</a>."},"date_updated":"2026-07-06T12:14:33Z","status":"public","author":[{"full_name":"Ucar, Mehmet C","orcid":"0000-0003-0506-4217","first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425","last_name":"Ucar"},{"first_name":"Reinhard","last_name":"Lipowsky","full_name":"Lipowsky, Reinhard"}],"fulldoi":"https://doi.org/10.1021/acs.nanolett.9b04445.s002","doi":"10.1021/acs.nanolett.9b04445.s002","day":"08","date_published":"2020-01-08T00:00:00Z","title":"MURL_Dataz","abstract":[{"lang":"eng","text":"Data obtained from the fine-grained simulations used in Figures 2-5, data obtained from the coarse-grained numerical calculations used in Figure 6, and a sample script for the fine-grained simulation as a Jupyter notebook (ZIP)"}]},{"isi":1,"arxiv":1,"fulldoi":"https://doi.org/10.1098/rsif.2019.0623","publication_status":"published","title":"Decomposing information into copying versus transformation","type":"journal_article","status":"public","language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"article_processing_charge":"No","abstract":[{"lang":"eng","text":"In many real-world systems, information can be transmitted in two qualitatively different ways: by copying or by transformation. Copying occurs when messages are transmitted without modification, e.g. when an offspring receives an unaltered copy of a gene from its parent. Transformation occurs when messages are modified systematically during transmission, e.g. when mutational biases occur during genetic replication. Standard information-theoretic measures do not distinguish these two modes of information transfer, although they may reflect different mechanisms and have different functional consequences. Starting from a few simple axioms, we derive a decomposition of mutual information into the information transmitted by copying versus the information transmitted by transformation. We begin with a decomposition that applies when the source and destination of the channel have the same set of messages and a notion of message identity exists. We then generalize our decomposition to other kinds of channels, which can involve different source and destination sets and broader notions of similarity. In addition, we show that copy information can be interpreted as the minimal work needed by a physical copying process, which is relevant for understanding the physics of replication. We use the proposed decomposition to explore a model of amino acid substitution rates. Our results apply to any system in which the fidelity of copying, rather than simple predictability, is of critical relevance."}],"scopus_import":"1","date_published":"2020-01-29T00:00:00Z","day":"29","doi":"10.1098/rsif.2019.0623","citation":{"chicago":"Kolchinsky, Artemy, and Bernat Corominas-Murtra. “Decomposing Information into Copying versus Transformation.” <i>Journal of the Royal Society Interface</i>. Royal Society, 2020. <a href=\"https://doi.org/10.1098/rsif.2019.0623\">https://doi.org/10.1098/rsif.2019.0623</a>.","ieee":"A. Kolchinsky and B. Corominas-Murtra, “Decomposing information into copying versus transformation,” <i>Journal of the Royal Society Interface</i>, vol. 17, no. 162. Royal Society, 2020.","short":"A. Kolchinsky, B. Corominas-Murtra, Journal of the Royal Society Interface 17 (2020).","mla":"Kolchinsky, Artemy, and Bernat Corominas-Murtra. “Decomposing Information into Copying versus Transformation.” <i>Journal of the Royal Society Interface</i>, vol. 17, no. 162, 0623, Royal Society, 2020, doi:<a href=\"https://doi.org/10.1098/rsif.2019.0623\">10.1098/rsif.2019.0623</a>.","ista":"Kolchinsky A, Corominas-Murtra B. 2020. Decomposing information into copying versus transformation. Journal of the Royal Society Interface. 17(162), 0623.","ama":"Kolchinsky A, Corominas-Murtra B. Decomposing information into copying versus transformation. <i>Journal of the Royal Society Interface</i>. 2020;17(162). doi:<a href=\"https://doi.org/10.1098/rsif.2019.0623\">10.1098/rsif.2019.0623</a>","apa":"Kolchinsky, A., &#38; Corominas-Murtra, B. (2020). Decomposing information into copying versus transformation. <i>Journal of the Royal Society Interface</i>. Royal Society. <a href=\"https://doi.org/10.1098/rsif.2019.0623\">https://doi.org/10.1098/rsif.2019.0623</a>"},"date_created":"2020-02-02T23:01:03Z","year":"2020","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1903.10693"}],"publication":"Journal of the Royal Society Interface","author":[{"last_name":"Kolchinsky","first_name":"Artemy","full_name":"Kolchinsky, Artemy"},{"last_name":"Corominas-Murtra","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","first_name":"Bernat","orcid":"0000-0001-9806-5643","full_name":"Corominas-Murtra, Bernat"}],"date_updated":"2026-08-12T14:08:47Z","intvolume":"        17","publication_identifier":{"eissn":["1742-5662"]},"article_type":"original","volume":17,"quality_controlled":"1","issue":"162","article_number":"0623","pmid":1,"acknowledgement":"AK was supported by Grant No. FQXi-RFP-1622 from the FQXi foundation, and Grant No. CHE-1648973 from the U.S.\r\nNational Science Foundation. AK would like to thank the Santa Fe Institute for supporting this research. The authors\r\nthank Jordi Fortuny, Rudolf Hanel, Joshua Garland, and Blai Vidiella for helpful discussions, as well as the anonymous\r\nreviewers for their insightful suggestions. ","department":[{"_id":"EdHa"}],"_id":"7431","publisher":"Royal Society","oa_version":"Preprint","month":"01","external_id":{"pmid":["31964273"],"isi":["000538369800002"],"arxiv":["1903.10693"]}},{"article_type":"original","publication_identifier":{"issn":["1465-7392"]},"volume":21,"intvolume":"        21","pmid":1,"quality_controlled":"1","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"_id":"5789","external_id":{"isi":["000457468300011"],"pmid":["30559456"]},"publisher":"Nature Publishing Group","oa_version":"Submitted Version","month":"02","acknowledged_ssus":[{"_id":"Bio"}],"date_published":"2019-02-01T00:00:00Z","day":"01","doi":"10.1038/s41556-018-0247-4","abstract":[{"text":"Tissue morphogenesis is driven by mechanical forces that elicit changes in cell size, shape and motion. The extent by which forces deform tissues critically depends on the rheological properties of the recipient tissue. Yet, whether and how dynamic changes in tissue rheology affect tissue morphogenesis and how they are regulated within the developing organism remain unclear. Here, we show that blastoderm spreading at the onset of zebrafish morphogenesis relies on a rapid, pronounced and spatially patterned tissue fluidization. Blastoderm fluidization is temporally controlled by mitotic cell rounding-dependent cell–cell contact disassembly during the last rounds of cell cleavages. Moreover, fluidization is spatially restricted to the central blastoderm by local activation of non-canonical Wnt signalling within the blastoderm margin, increasing cell cohesion and thereby counteracting the effect of mitotic rounding on contact disassembly. Overall, our results identify a fluidity transition mediated by loss of cell cohesion as a critical regulator of embryo morphogenesis.","lang":"eng"}],"scopus_import":"1","publication":"Nature Cell Biology","citation":{"ieee":"N. Petridou, S. Grigolon, G. Salbreux, E. B. Hannezo, and C.-P. J. Heisenberg, “Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling,” <i>Nature Cell Biology</i>, vol. 21. Nature Publishing Group, pp. 169–178, 2019.","short":"N. Petridou, S. Grigolon, G. Salbreux, E.B. Hannezo, C.-P.J. Heisenberg, Nature Cell Biology 21 (2019) 169–178.","mla":"Petridou, Nicoletta, et al. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” <i>Nature Cell Biology</i>, vol. 21, Nature Publishing Group, 2019, pp. 169–178, doi:<a href=\"https://doi.org/10.1038/s41556-018-0247-4\">10.1038/s41556-018-0247-4</a>.","chicago":"Petridou, Nicoletta, Silvia Grigolon, Guillaume Salbreux, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2019. <a href=\"https://doi.org/10.1038/s41556-018-0247-4\">https://doi.org/10.1038/s41556-018-0247-4</a>.","apa":"Petridou, N., Grigolon, S., Salbreux, G., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2019). Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41556-018-0247-4\">https://doi.org/10.1038/s41556-018-0247-4</a>","ama":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. <i>Nature Cell Biology</i>. 2019;21:169–178. doi:<a href=\"https://doi.org/10.1038/s41556-018-0247-4\">10.1038/s41556-018-0247-4</a>","ista":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. 2019. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. 21, 169–178."},"date_created":"2018-12-30T22:59:15Z","year":"2019","date_updated":"2025-07-10T11:52:59Z","author":[{"first_name":"Nicoletta","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","last_name":"Petridou","full_name":"Petridou, Nicoletta","orcid":"0000-0002-8451-1195"},{"full_name":"Grigolon, Silvia","first_name":"Silvia","last_name":"Grigolon"},{"full_name":"Salbreux, Guillaume","first_name":"Guillaume","last_name":"Salbreux"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"169–178","file_date_updated":"2020-10-21T07:18:35Z","has_accepted_license":"1","article_processing_charge":"No","related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/when-a-fish-becomes-fluid/"}]},"oa":1,"fulldoi":"https://doi.org/10.1038/s41556-018-0247-4","isi":1,"publication_status":"published","file":[{"file_size":71590590,"file_id":"8685","checksum":"e38523787b3bc84006f2793de99ad70f","creator":"dernst","success":1,"date_updated":"2020-10-21T07:18:35Z","relation":"main_file","access_level":"open_access","file_name":"2018_NatureCellBio_Petridou_accepted.pdf","content_type":"application/pdf","date_created":"2020-10-21T07:18:35Z"}],"title":"Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","call_identifier":"H2020"},{"name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants","_id":"253E54C8-B435-11E9-9278-68D0E5697425","grant_number":"ALTF710-2016"}],"type":"journal_article","ddc":["570"],"ec_funded":1,"status":"public"},{"publication_status":"published","title":"Thermodynamics of duplication thresholds in synthetic protocell systems","file":[{"date_created":"2019-02-11T10:45:27Z","content_type":"application/pdf","file_name":"2019_Life_Corominas.pdf","access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:47:13Z","creator":"dernst","checksum":"7d2322cd96ace41959909b66702d5cf4","file_id":"5951","file_size":963454}],"isi":1,"fulldoi":"https://doi.org/10.3390/life9010009","status":"public","ddc":["570"],"type":"journal_article","oa":1,"article_processing_charge":"No","has_accepted_license":"1","file_date_updated":"2020-07-14T12:47:13Z","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","scopus_import":"1","abstract":[{"lang":"eng","text":"Understanding the thermodynamics of the duplication process is a fundamental step towards a comprehensive physical theory of biological systems. However, the immense complexity of real cells obscures the fundamental tensions between energy gradients and entropic contributions that underlie duplication. The study of synthetic, feasible systems reproducing part of the key ingredients of living entities but overcoming major sources of biological complexity is of great relevance to deepen the comprehension of the fundamental thermodynamic processes underlying life and its prevalence. In this paper an abstract—yet realistic—synthetic system made of small synthetic protocell aggregates is studied in detail. A fundamental relation between free energy and entropic gradients is derived for a general, non-equilibrium scenario, setting the thermodynamic conditions for the occurrence and prevalence of duplication phenomena. This relation sets explicitly how the energy gradients invested in creating and maintaining structural—and eventually, functional—elements of the system must always compensate the entropic gradients, whose contributions come from changes in the translational, configurational, and macrostate entropies, as well as from dissipation due to irreversible transitions. Work/energy relations are also derived, defining lower bounds on the energy required for the duplication event to take place. A specific example including real ternary emulsions is provided in order to grasp the orders of magnitude involved in the problem. It is found that the minimal work invested over the system to trigger a duplication event is around ~ 10−13J , which results, in the case of duplication of all the vesicles contained in a liter of emulsion, in an amount of energy around ~ 1kJ . Without aiming to describe a truly biological process of duplication, this theoretical contribution seeks to explicitly define and identify the key actors that participate in it."}],"day":"15","date_published":"2019-01-15T00:00:00Z","doi":"10.3390/life9010009","author":[{"id":"43BE2298-F248-11E8-B48F-1D18A9856A87","first_name":"Bernat","last_name":"Corominas-Murtra","full_name":"Corominas-Murtra, Bernat","orcid":"0000-0001-9806-5643"}],"date_updated":"2026-04-03T09:37:52Z","citation":{"ista":"Corominas-Murtra B. 2019. Thermodynamics of duplication thresholds in synthetic protocell systems. Life. 9(1), 9.","ama":"Corominas-Murtra B. Thermodynamics of duplication thresholds in synthetic protocell systems. <i>Life</i>. 2019;9(1). doi:<a href=\"https://doi.org/10.3390/life9010009\">10.3390/life9010009</a>","apa":"Corominas-Murtra, B. (2019). Thermodynamics of duplication thresholds in synthetic protocell systems. <i>Life</i>. MDPI. <a href=\"https://doi.org/10.3390/life9010009\">https://doi.org/10.3390/life9010009</a>","chicago":"Corominas-Murtra, Bernat. “Thermodynamics of Duplication Thresholds in Synthetic Protocell Systems.” <i>Life</i>. MDPI, 2019. <a href=\"https://doi.org/10.3390/life9010009\">https://doi.org/10.3390/life9010009</a>.","ieee":"B. Corominas-Murtra, “Thermodynamics of duplication thresholds in synthetic protocell systems,” <i>Life</i>, vol. 9, no. 1. MDPI, 2019.","short":"B. Corominas-Murtra, Life 9 (2019).","mla":"Corominas-Murtra, Bernat. “Thermodynamics of Duplication Thresholds in Synthetic Protocell Systems.” <i>Life</i>, vol. 9, no. 1, 9, MDPI, 2019, doi:<a href=\"https://doi.org/10.3390/life9010009\">10.3390/life9010009</a>."},"year":"2019","date_created":"2019-02-10T22:59:15Z","publication":"Life","quality_controlled":"1","issue":"1","article_number":"9","intvolume":"         9","publication_identifier":{"eissn":["2075-1729"]},"volume":9,"publisher":"MDPI","month":"01","oa_version":"Published Version","external_id":{"isi":["000464125500001"]},"department":[{"_id":"EdHa"}],"_id":"5944"},{"type":"journal_article","ddc":["570"],"status":"public","fulldoi":"https://doi.org/10.1073/pnas.1813255116","isi":1,"file":[{"creator":"dernst","checksum":"8b67eee0ea8e5db61583e4d485215258","file_id":"6193","file_size":3456045,"date_created":"2019-04-03T14:10:30Z","content_type":"application/pdf","file_name":"2019_PNAS_Recho.pdf","access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:47:23Z"}],"project":[{"call_identifier":"FWF","grant_number":"P31639","_id":"268294B6-B435-11E9-9278-68D0E5697425","name":"Active mechano-chemical description of the cell cytoskeleton"}],"title":"Theory of mechanochemical patterning in biphasic biological tissues","publication_status":"published","page":"5344-5349","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"language":[{"iso":"eng"}],"article_processing_charge":"No","has_accepted_license":"1","file_date_updated":"2020-07-14T12:47:23Z","oa":1,"related_material":{"link":[{"relation":"supplementary_material","url":"www.pnas.org/lookup/suppl/doi:10.1073/pnas.1813255116/-/DCSupplemental"}]},"corr_author":"1","publication":"Proceedings of the National Academy of Sciences of the United States of America","date_created":"2019-03-31T21:59:13Z","year":"2019","citation":{"mla":"Recho, Pierre, et al. “Theory of Mechanochemical Patterning in Biphasic Biological Tissues.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 116, no. 12, National Academy of Sciences, 2019, pp. 5344–49, doi:<a href=\"https://doi.org/10.1073/pnas.1813255116\">10.1073/pnas.1813255116</a>.","ieee":"P. Recho, A. Hallou, and E. B. Hannezo, “Theory of mechanochemical patterning in biphasic biological tissues,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 116, no. 12. National Academy of Sciences, pp. 5344–5349, 2019.","short":"P. Recho, A. Hallou, E.B. Hannezo, Proceedings of the National Academy of Sciences of the United States of America 116 (2019) 5344–5349.","chicago":"Recho, Pierre, Adrien Hallou, and Edouard B Hannezo. “Theory of Mechanochemical Patterning in Biphasic Biological Tissues.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2019. <a href=\"https://doi.org/10.1073/pnas.1813255116\">https://doi.org/10.1073/pnas.1813255116</a>.","apa":"Recho, P., Hallou, A., &#38; Hannezo, E. B. (2019). Theory of mechanochemical patterning in biphasic biological tissues. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1813255116\">https://doi.org/10.1073/pnas.1813255116</a>","ista":"Recho P, Hallou A, Hannezo EB. 2019. Theory of mechanochemical patterning in biphasic biological tissues. Proceedings of the National Academy of Sciences of the United States of America. 116(12), 5344–5349.","ama":"Recho P, Hallou A, Hannezo EB. Theory of mechanochemical patterning in biphasic biological tissues. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2019;116(12):5344-5349. doi:<a href=\"https://doi.org/10.1073/pnas.1813255116\">10.1073/pnas.1813255116</a>"},"date_updated":"2025-07-10T11:53:14Z","author":[{"full_name":"Recho, Pierre","first_name":"Pierre","last_name":"Recho"},{"full_name":"Hallou, Adrien","last_name":"Hallou","first_name":"Adrien"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo"}],"doi":"10.1073/pnas.1813255116","day":"19","date_published":"2019-03-19T00:00:00Z","abstract":[{"lang":"eng","text":"The formation of self-organized patterns is key to the morphogenesis of multicellular organisms, although a comprehensive theory of biological pattern formation is still lacking. Here, we propose a minimal model combining tissue mechanics with morphogen turnover and transport to explore routes to patterning. Our active description couples morphogen reaction and diffusion, which impact cell differentiation and tissue mechanics, to a two-phase poroelastic rheology, where one tissue phase consists of a poroelastic cell network and the other one of a permeating extracellular fluid, which provides a feedback by actively transporting morphogens. While this model encompasses previous theories approximating tissues to inert monophasic media, such as Turing’s reaction–diffusion model, it overcomes some of their key limitations permitting pattern formation via any two-species biochemical kinetics due to mechanically induced cross-diffusion flows. Moreover, we describe a qualitatively different advection-driven Keller–Segel instability which allows for the formation of patterns with a single morphogen and whose fundamental mode pattern robustly scales with tissue size. We discuss the potential relevance of these findings for tissue morphogenesis."}],"scopus_import":"1","_id":"6191","department":[{"_id":"EdHa"}],"external_id":{"pmid":["30819884"],"isi":["000461679000027"]},"oa_version":"Published Version","month":"03","publisher":"National Academy of Sciences","volume":116,"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"intvolume":"       116","issue":"12","pmid":1,"quality_controlled":"1"}]