[{"volume":5,"oa_version":"Submitted Version","project":[{"call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"}],"ec_funded":1,"date_updated":"2025-04-15T06:50:24Z","title":"A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis","day":"08","author":[{"full_name":"Yoshida, Saiko","last_name":"Yoshida","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko"},{"last_name":"Van Der Schuren","full_name":"Van Der Schuren, Alja","first_name":"Alja"},{"full_name":"Van Dop, Maritza","last_name":"Van Dop","first_name":"Maritza"},{"last_name":"Van Galen","full_name":"Van Galen, Luc","first_name":"Luc"},{"full_name":"Saiga, Shunsuke","last_name":"Saiga","first_name":"Shunsuke"},{"last_name":"Adibi","full_name":"Adibi, Milad","first_name":"Milad"},{"first_name":"Barbara","last_name":"Möller","full_name":"Möller, Barbara"},{"full_name":"Ten Hove, Colette A.","last_name":"Ten Hove","first_name":"Colette A."},{"id":"3F45B078-F248-11E8-B48F-1D18A9856A87","last_name":"Marhavy","full_name":"Marhavy, Peter","orcid":"0000-0001-5227-5741","first_name":"Peter"},{"first_name":"Richard","last_name":"Smith","full_name":"Smith, Richard"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"},{"first_name":"Dolf","full_name":"Weijers, Dolf","last_name":"Weijers"}],"doi":"10.1038/s41477-019-0363-6","scopus_import":"1","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"language":[{"iso":"eng"}],"external_id":{"isi":["000460479600014"]},"status":"public","oa":1,"issue":"2","article_processing_charge":"No","publisher":"Springer Nature","_id":"6023","date_published":"2019-02-08T00:00:00Z","publication_status":"published","intvolume":"         5","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","isi":1,"type":"journal_article","publication":"Nature Plants","main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/479113v1.abstract","open_access":"1"}],"page":"160-166","year":"2019","quality_controlled":"1","citation":{"ieee":"S. Yoshida <i>et al.</i>, “A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis,” <i>Nature Plants</i>, vol. 5, no. 2. Springer Nature, pp. 160–166, 2019.","chicago":"Yoshida, Saiko, Alja Van Der Schuren, Maritza Van Dop, Luc Van Galen, Shunsuke Saiga, Milad Adibi, Barbara Möller, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” <i>Nature Plants</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41477-019-0363-6\">https://doi.org/10.1038/s41477-019-0363-6</a>.","short":"S. Yoshida, A. Van Der Schuren, M. Van Dop, L. Van Galen, S. Saiga, M. Adibi, B. Möller, C.A. Ten Hove, P. Marhavý, R. Smith, J. Friml, D. Weijers, Nature Plants 5 (2019) 160–166.","apa":"Yoshida, S., Van Der Schuren, A., Van Dop, M., Van Galen, L., Saiga, S., Adibi, M., … Weijers, D. (2019). A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-019-0363-6\">https://doi.org/10.1038/s41477-019-0363-6</a>","mla":"Yoshida, Saiko, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” <i>Nature Plants</i>, vol. 5, no. 2, Springer Nature, 2019, pp. 160–66, doi:<a href=\"https://doi.org/10.1038/s41477-019-0363-6\">10.1038/s41477-019-0363-6</a>.","ama":"Yoshida S, Van Der Schuren A, Van Dop M, et al. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. <i>Nature Plants</i>. 2019;5(2):160-166. doi:<a href=\"https://doi.org/10.1038/s41477-019-0363-6\">10.1038/s41477-019-0363-6</a>","ista":"Yoshida S, Van Der Schuren A, Van Dop M, Van Galen L, Saiga S, Adibi M, Möller B, Ten Hove CA, Marhavý P, Smith R, Friml J, Weijers D. 2019. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. Nature Plants. 5(2), 160–166."},"abstract":[{"text":"Multicellular development requires coordinated cell polarization relative to body axes, and translation to oriented cell division 1–3 . In plants, it is unknown how cell polarities are connected to organismal axes and translated to division. Here, we identify Arabidopsis SOSEKI proteins that integrate apical–basal and radial organismal axes to localize to polar cell edges. Localization does not depend on tissue context, requires cell wall integrity and is defined by a transferrable, protein-specific motif. A Domain of Unknown Function in SOSEKI proteins resembles the DIX oligomerization domain in the animal Dishevelled polarity regulator. The DIX-like domain self-interacts and is required for edge localization and for influencing division orientation, together with a second domain that defines the polar membrane domain. Our work shows that SOSEKI proteins locally interpret global polarity cues and can influence cell division orientation. Furthermore, this work reveals that, despite fundamental differences, cell polarity mechanisms in plants and animals converge on a similar protein domain.","lang":"eng"}],"date_created":"2019-02-17T22:59:21Z","month":"02"},{"publication_identifier":{"eissn":["1674-2052"]},"publication_status":"published","intvolume":"        12","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","isi":1,"page":"1312-1314","type":"journal_article","publication":"Molecular Plant","date_created":"2019-09-30T10:00:40Z","citation":{"chicago":"Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners in Root Growth Regulation.” <i>Molecular Plant</i>. Cell Press, 2019. <a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">https://doi.org/10.1016/j.molp.2019.09.003</a>.","ieee":"C. Artner and E. Benková, “Ethylene and cytokinin - partners in root growth regulation,” <i>Molecular Plant</i>, vol. 12, no. 10. Cell Press, pp. 1312–1314, 2019.","ista":"Artner C, Benková E. 2019. Ethylene and cytokinin - partners in root growth regulation. Molecular Plant. 12(10), 1312–1314.","ama":"Artner C, Benková E. Ethylene and cytokinin - partners in root growth regulation. <i>Molecular Plant</i>. 2019;12(10):1312-1314. doi:<a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">10.1016/j.molp.2019.09.003</a>","short":"C. Artner, E. Benková, Molecular Plant 12 (2019) 1312–1314.","mla":"Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners in Root Growth Regulation.” <i>Molecular Plant</i>, vol. 12, no. 10, Cell Press, 2019, pp. 1312–14, doi:<a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">10.1016/j.molp.2019.09.003</a>.","apa":"Artner, C., &#38; Benková, E. (2019). Ethylene and cytokinin - partners in root growth regulation. <i>Molecular Plant</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">https://doi.org/10.1016/j.molp.2019.09.003</a>"},"year":"2019","quality_controlled":"1","month":"10","oa_version":"None","volume":12,"article_type":"original","title":"Ethylene and cytokinin - partners in root growth regulation","date_updated":"2025-07-10T11:54:01Z","project":[{"name":"Hormonal regulation of plant adaptive responses to environmental signals","_id":"2685A872-B435-11E9-9278-68D0E5697425"}],"doi":"10.1016/j.molp.2019.09.003","scopus_import":"1","author":[{"first_name":"Christina","id":"45DF286A-F248-11E8-B48F-1D18A9856A87","last_name":"Artner","full_name":"Artner, Christina"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739"}],"day":"07","language":[{"iso":"eng"}],"department":[{"_id":"EvBe"}],"status":"public","external_id":{"pmid":["31541740"],"isi":["000489132500002"]},"pmid":1,"publisher":"Cell Press","article_processing_charge":"No","issue":"10","date_published":"2019-10-07T00:00:00Z","_id":"6920"},{"isi":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","intvolume":"        52","publication_identifier":{"issn":["1369-5266"]},"publication_status":"published","month":"12","date_created":"2020-01-29T16:00:07Z","quality_controlled":"1","citation":{"ista":"Benková E, Dagdas Y. 2019. Editorial overview: Cell biology in the era of omics? Current Opinion in Plant Biology. 52(12), A1–A2.","apa":"Benková, E., &#38; Dagdas, Y. (2019). Editorial overview: Cell biology in the era of omics? <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">https://doi.org/10.1016/j.pbi.2019.11.002</a>","mla":"Benková, Eva, and Yasin Dagdas. “Editorial Overview: Cell Biology in the Era of Omics?” <i>Current Opinion in Plant Biology</i>, vol. 52, no. 12, Elsevier, 2019, pp. A1–2, doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">10.1016/j.pbi.2019.11.002</a>.","short":"E. Benková, Y. Dagdas, Current Opinion in Plant Biology 52 (2019) A1–A2.","ama":"Benková E, Dagdas Y. Editorial overview: Cell biology in the era of omics? <i>Current Opinion in Plant Biology</i>. 2019;52(12):A1-A2. doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">10.1016/j.pbi.2019.11.002</a>","chicago":"Benková, Eva, and Yasin Dagdas. “Editorial Overview: Cell Biology in the Era of Omics?” <i>Current Opinion in Plant Biology</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">https://doi.org/10.1016/j.pbi.2019.11.002</a>.","ieee":"E. Benková and Y. Dagdas, “Editorial overview: Cell biology in the era of omics?,” <i>Current Opinion in Plant Biology</i>, vol. 52, no. 12. Elsevier, pp. A1–A2, 2019."},"year":"2019","page":"A1-A2","type":"journal_article","publication":"Current Opinion in Plant Biology","language":[{"iso":"eng"}],"department":[{"_id":"EvBe"}],"scopus_import":"1","doi":"10.1016/j.pbi.2019.11.002","author":[{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739"},{"full_name":"Dagdas, Yasin","last_name":"Dagdas","first_name":"Yasin"}],"day":"01","date_updated":"2023-09-07T14:56:55Z","title":"Editorial overview: Cell biology in the era of omics?","oa_version":"None","volume":52,"article_type":"letter_note","date_published":"2019-12-01T00:00:00Z","_id":"7394","publisher":"Elsevier","article_processing_charge":"No","issue":"12","external_id":{"pmid":["31787165"],"isi":["000502890600001"]},"status":"public","pmid":1},{"type":"journal_article","publication":"Development","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1242/dev.175919"}],"year":"2019","quality_controlled":"1","citation":{"ista":"Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. 2019. Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. Development. 146(17), dev175919.","ama":"Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. <i>Development</i>. 2019;146(17). doi:<a href=\"https://doi.org/10.1242/dev.175919\">10.1242/dev.175919</a>","apa":"Zhu, Q., Gallemi, M., Pospíšil, J., Žádníková, P., Strnad, M., &#38; Benková, E. (2019). Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.175919\">https://doi.org/10.1242/dev.175919</a>","short":"Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, E. Benková, Development 146 (2019).","mla":"Zhu, Qiang, et al. “Root Gravity Response Module Guides Differential Growth Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” <i>Development</i>, vol. 146, no. 17, dev175919, Company of Biologists, 2019, doi:<a href=\"https://doi.org/10.1242/dev.175919\">10.1242/dev.175919</a>.","chicago":"Zhu, Qiang, Marçal Gallemi, Jiří Pospíšil, Petra Žádníková, Miroslav Strnad, and Eva Benková. “Root Gravity Response Module Guides Differential Growth Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” <i>Development</i>. Company of Biologists, 2019. <a href=\"https://doi.org/10.1242/dev.175919\">https://doi.org/10.1242/dev.175919</a>.","ieee":"Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, and E. Benková, “Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis,” <i>Development</i>, vol. 146, no. 17. Company of Biologists, 2019."},"date_created":"2019-09-22T22:00:36Z","abstract":[{"text":"The apical hook is a transiently formed structure that plays a protective role when the germinating seedling penetrates through the soil towards the surface. Crucial for proper bending is the local auxin maxima, which defines the concave (inner) side of the hook curvature. As no sign of asymmetric auxin distribution has been reported in embryonic hypocotyls prior to hook formation, the question of how auxin asymmetry is established in the early phases of seedling germination remains largely unanswered. Here, we analyzed the auxin distribution and expression of PIN auxin efflux carriers from early phases of germination, and show that bending of the root in response to gravity is the crucial initial cue that governs the hypocotyl bending required for apical hook formation. Importantly, polar auxin transport machinery is established gradually after germination starts as a result of tight root-hypocotyl interaction and a proper balance between abscisic acid and gibberellins.","lang":"eng"}],"month":"09","publication_status":"published","publication_identifier":{"eissn":["1477-9129"]},"intvolume":"       146","isi":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","external_id":{"isi":["000486297400011"],"pmid":["31391194"]},"pmid":1,"article_processing_charge":"No","acknowledgement":"We thank Jiri Friml and Phillip Brewer for inspiring discussion and for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility\r\n(BIF), the Life Science Facility (LSF).\r\nThis work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to E.B.). J.P. and M.S. received funds from European Regional Development Fund-Project ‘Centre for Experimental Plant Biology’ (No. CZ.02.1.01/0.0/0.0/16_019/0000738).","oa":1,"issue":"17","publisher":"Company of Biologists","_id":"6897","date_published":"2019-09-12T00:00:00Z","article_type":"original","volume":146,"oa_version":"Published Version","article_number":"dev175919","ec_funded":1,"project":[{"grant_number":"207362","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","name":"Hormonal cross-talk in plant organogenesis","call_identifier":"FP7"}],"date_updated":"2026-08-12T09:57:36Z","title":"Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis","author":[{"last_name":"Zhu","full_name":"Zhu, Qiang","id":"40A4B9E6-F248-11E8-B48F-1D18A9856A87","first_name":"Qiang"},{"id":"460C6802-F248-11E8-B48F-1D18A9856A87","last_name":"Gallemi","full_name":"Gallemi, Marçal","orcid":"0000-0003-4675-6893","first_name":"Marçal"},{"first_name":"Jiří","last_name":"Pospíšil","full_name":"Pospíšil, Jiří"},{"full_name":"Žádníková, Petra","last_name":"Žádníková","first_name":"Petra"},{"first_name":"Miroslav","last_name":"Strnad","full_name":"Strnad, Miroslav"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"day":"12","ddc":["580"],"doi":"10.1242/dev.175919","scopus_import":"1","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}]},{"pmid":1,"external_id":{"pmid":["31051107"],"isi":["000466843000015"]},"file":[{"checksum":"4ceba04a96a74f5092ec3ce2c579a0c7","creator":"dernst","content_type":"application/pdf","access_level":"open_access","file_size":10272032,"file_name":"2019_Cell_Marhava.pdf","relation":"main_file","date_updated":"2020-07-14T12:47:28Z","date_created":"2019-05-13T06:12:45Z","file_id":"6411"}],"status":"public","publisher":"Elsevier","oa":1,"issue":"4","article_processing_charge":"No","corr_author":"1","date_published":"2019-05-02T00:00:00Z","_id":"6351","oa_version":"Published Version","volume":177,"file_date_updated":"2020-07-14T12:47:28Z","date_updated":"2026-09-01T22:30:26Z","title":"Re-activation of stem cell pathways for pattern restoration in plant wound healing","ec_funded":1,"project":[{"call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"has_accepted_license":"1","scopus_import":"1","doi":"10.1016/j.cell.2019.04.015","ddc":["570"],"day":"02","author":[{"last_name":"Marhavá","full_name":"Marhavá, Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87","first_name":"Petra"},{"id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","last_name":"Hörmayer","full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926","first_name":"Lukas"},{"last_name":"Yoshida","full_name":"Yoshida, Saiko","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko","orcid":"0000-0001-6111-9353"},{"orcid":"0000-0001-5227-5741","first_name":"Peter","last_name":"Marhavy","full_name":"Marhavy, Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml"}],"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"related_material":{"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/","relation":"press_release"}],"record":[{"relation":"dissertation_contains","status":"public","id":"9992"}]},"acknowledged_ssus":[{"_id":"Bio"}],"page":"957-969.e13","publication":"Cell","type":"journal_article","abstract":[{"text":"A process of restorative patterning in plant roots correctly replaces eliminated cells to heal local injuries despite the absence of cell migration, which underpins wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell divisions and acquisition of specific cell identities. Plants regularly endure wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary abilities to restore their tissues after injuries. Here, we provide insight into a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted elimination of different cells in Arabidopsis root combined with live-imaging tracking during vertical growth allowed analysis of the regeneration processes in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated their stem cell transcriptional programs. They accelerated their progression through cell cycle, coordinately changed the cell division orientation, and ultimately acquired de novo the correct cell fates to replace missing cells. These observations highlight existence of unknown intercellular positional signaling and demonstrate the capability of specified cells to re-acquire stem cell programs as a crucial part of the plant-specific mechanism of wound healing.","lang":"eng"}],"date_created":"2019-04-28T21:59:14Z","citation":{"chicago":"Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková, and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” <i>Cell</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">https://doi.org/10.1016/j.cell.2019.04.015</a>.","ieee":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml, “Re-activation of stem cell pathways for pattern restoration in plant wound healing,” <i>Cell</i>, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.","ista":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4), 957–969.e13.","ama":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation of stem cell pathways for pattern restoration in plant wound healing. <i>Cell</i>. 2019;177(4):957-969.e13. doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">10.1016/j.cell.2019.04.015</a>","short":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell 177 (2019) 957–969.e13.","apa":"Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., &#38; Friml, J. (2019). Re-activation of stem cell pathways for pattern restoration in plant wound healing. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">https://doi.org/10.1016/j.cell.2019.04.015</a>","mla":"Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” <i>Cell</i>, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13, doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">10.1016/j.cell.2019.04.015</a>."},"year":"2019","quality_controlled":"1","month":"05","publication_status":"published","publication_identifier":{"issn":["0092-8674"],"eissn":["1097-4172"]},"intvolume":"       177","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}},{"date_created":"2018-12-11T11:45:34Z","publist_id":"7625","abstract":[{"lang":"eng","text":"Arabidopsis and human ARM protein interact with telomerase. Deregulated mRNA levels of DNA repair and ribosomal protein genes in an Arabidopsis arm mutant suggest non-telomeric ARM function. The human homolog ARMC6 interacts with hTRF2. Abstract: Telomerase maintains telomeres and has proposed non-telomeric functions. We previously identified interaction of the C-terminal domain of Arabidopsis telomerase reverse transcriptase (AtTERT) with an armadillo/β-catenin-like repeat (ARM) containing protein. Here we explore protein–protein interactions of the ARM protein, AtTERT domains, POT1a, TRF-like family and SMH family proteins, and the chromatin remodeling protein CHR19 using bimolecular fluorescence complementation (BiFC), yeast two-hybrid (Y2H) analysis, and co-immunoprecipitation. The ARM protein interacts with both the N- and C-terminal domains of AtTERT in different cellular compartments. ARM interacts with CHR19 and TRF-like I family proteins that also bind AtTERT directly or through interaction with POT1a. The putative human ARM homolog co-precipitates telomerase activity and interacts with hTRF2 protein in vitro. Analysis of Arabidopsis arm mutants shows no obvious changes in telomere length or telomerase activity, suggesting that ARM is not essential for telomere maintenance. The observed interactions with telomerase and Myb-like domain proteins (TRF-like family I) may therefore reflect possible non-telomeric functions. Transcript levels of several DNA repair and ribosomal genes are affected in arm mutants, and ARM, likely in association with other proteins, suppressed expression of XRCC3 and RPSAA promoter constructs in luciferase reporter assays. In conclusion, ARM can participate in non-telomeric functions of telomerase, and can also perform its own telomerase-independent functions."}],"citation":{"chicago":"Dokládal, Ladislav, Eva Benková, David Honys, Nikoleta Dupláková, Lan Lee, Stanton Gelvin, and Eva Sýkorová. “An Armadillo-Domain Protein Participates in a Telomerase Interaction Network.” <i>Plant Molecular Biology</i>. Springer, 2018. <a href=\"https://doi.org/10.1007/s11103-018-0747-4\">https://doi.org/10.1007/s11103-018-0747-4</a>.","ieee":"L. Dokládal <i>et al.</i>, “An armadillo-domain protein participates in a telomerase interaction network,” <i>Plant Molecular Biology</i>, vol. 97, no. 5. Springer, pp. 407–420, 2018.","ista":"Dokládal L, Benková E, Honys D, Dupláková N, Lee L, Gelvin S, Sýkorová E. 2018. An armadillo-domain protein participates in a telomerase interaction network. Plant Molecular Biology. 97(5), 407–420.","apa":"Dokládal, L., Benková, E., Honys, D., Dupláková, N., Lee, L., Gelvin, S., &#38; Sýkorová, E. (2018). An armadillo-domain protein participates in a telomerase interaction network. <i>Plant Molecular Biology</i>. Springer. <a href=\"https://doi.org/10.1007/s11103-018-0747-4\">https://doi.org/10.1007/s11103-018-0747-4</a>","short":"L. Dokládal, E. Benková, D. Honys, N. Dupláková, L. Lee, S. Gelvin, E. Sýkorová, Plant Molecular Biology 97 (2018) 407–420.","mla":"Dokládal, Ladislav, et al. “An Armadillo-Domain Protein Participates in a Telomerase Interaction Network.” <i>Plant Molecular Biology</i>, vol. 97, no. 5, Springer, 2018, pp. 407–20, doi:<a href=\"https://doi.org/10.1007/s11103-018-0747-4\">10.1007/s11103-018-0747-4</a>.","ama":"Dokládal L, Benková E, Honys D, et al. An armadillo-domain protein participates in a telomerase interaction network. <i>Plant Molecular Biology</i>. 2018;97(5):407-420. doi:<a href=\"https://doi.org/10.1007/s11103-018-0747-4\">10.1007/s11103-018-0747-4</a>"},"year":"2018","quality_controlled":"1","page":"407 - 420","publication":"Plant Molecular Biology","type":"journal_article","month":"06","intvolume":"        97","publication_status":"published","isi":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Springer","article_processing_charge":"No","issue":"5","oa":1,"status":"public","file":[{"date_created":"2020-05-14T12:23:08Z","date_updated":"2020-07-14T12:45:45Z","relation":"main_file","file_name":"2018_PlantMolecBio_Dokladal.pdf","file_id":"7834","file_size":1150679,"content_type":"application/pdf","access_level":"open_access","creator":"dernst","checksum":"451ae47616e6af2533099f596b2a47fb"}],"external_id":{"isi":["000438981700009"]},"date_published":"2018-06-12T00:00:00Z","_id":"277","date_updated":"2023-09-08T13:21:05Z","title":"An armadillo-domain protein participates in a telomerase interaction network","oa_version":"Submitted Version","volume":97,"article_type":"original","file_date_updated":"2020-07-14T12:45:45Z","language":[{"iso":"eng"}],"department":[{"_id":"EvBe"}],"doi":"10.1007/s11103-018-0747-4","scopus_import":"1","has_accepted_license":"1","author":[{"full_name":"Dokládal, Ladislav","last_name":"Dokládal","first_name":"Ladislav"},{"full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739"},{"first_name":"David","full_name":"Honys, David","last_name":"Honys"},{"first_name":"Nikoleta","full_name":"Dupláková, Nikoleta","last_name":"Dupláková"},{"full_name":"Lee, Lan","last_name":"Lee","first_name":"Lan"},{"full_name":"Gelvin, Stanton","last_name":"Gelvin","first_name":"Stanton"},{"first_name":"Eva","last_name":"Sýkorová","full_name":"Sýkorová, Eva"}],"ddc":["580"],"day":"12"},{"article_processing_charge":"No","issue":"1","oa":1,"publisher":"Nature Publishing Group","external_id":{"isi":["000434640800008"]},"file":[{"creator":"dernst","access_level":"open_access","content_type":"application/pdf","checksum":"9c3942d772f84f3df032ffde0ed9a8ea","file_id":"5707","file_name":"2018_ScientificReports_Ceinos.pdf","relation":"main_file","date_created":"2018-12-17T13:04:46Z","date_updated":"2020-07-14T12:45:49Z","file_size":1855324}],"status":"public","_id":"283","date_published":"2018-06-08T00:00:00Z","date_updated":"2023-09-13T08:59:27Z","title":"Mutations in blind cavefish target the light regulated circadian clock gene period 2","file_date_updated":"2020-07-14T12:45:49Z","oa_version":"Published Version","volume":8,"article_number":"8754","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}],"author":[{"last_name":"Ceinos","full_name":"Ceinos, Rosa Maria","first_name":"Rosa Maria"},{"last_name":"Frigato","full_name":"Frigato, Elena","first_name":"Elena"},{"full_name":"Pagano, Cristina","last_name":"Pagano","first_name":"Cristina"},{"first_name":"Nadine","last_name":"Frohlich","full_name":"Frohlich, Nadine"},{"first_name":"Pietro","full_name":"Negrini, Pietro","last_name":"Negrini"},{"first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","last_name":"Cavallari","full_name":"Cavallari, Nicola"},{"last_name":"Vallone","full_name":"Vallone, Daniela","first_name":"Daniela"},{"first_name":"Silvia","last_name":"Fuselli","full_name":"Fuselli, Silvia"},{"first_name":"Cristiano","full_name":"Bertolucci, Cristiano","last_name":"Bertolucci"},{"last_name":"Foulkes","full_name":"Foulkes, Nicholas S","first_name":"Nicholas S"}],"day":"08","ddc":["570"],"doi":"10.1038/s41598-018-27080-2","scopus_import":"1","has_accepted_license":"1","quality_controlled":"1","citation":{"ieee":"R. M. Ceinos <i>et al.</i>, “Mutations in blind cavefish target the light regulated circadian clock gene period 2,” <i>Scientific Reports</i>, vol. 8, no. 1. Nature Publishing Group, 2018.","chicago":"Ceinos, Rosa Maria, Elena Frigato, Cristina Pagano, Nadine Frohlich, Pietro Negrini, Nicola Cavallari, Daniela Vallone, Silvia Fuselli, Cristiano Bertolucci, and Nicholas S Foulkes. “Mutations in Blind Cavefish Target the Light Regulated Circadian Clock Gene Period 2.” <i>Scientific Reports</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41598-018-27080-2\">https://doi.org/10.1038/s41598-018-27080-2</a>.","mla":"Ceinos, Rosa Maria, et al. “Mutations in Blind Cavefish Target the Light Regulated Circadian Clock Gene Period 2.” <i>Scientific Reports</i>, vol. 8, no. 1, 8754, Nature Publishing Group, 2018, doi:<a href=\"https://doi.org/10.1038/s41598-018-27080-2\">10.1038/s41598-018-27080-2</a>.","apa":"Ceinos, R. M., Frigato, E., Pagano, C., Frohlich, N., Negrini, P., Cavallari, N., … Foulkes, N. S. (2018). Mutations in blind cavefish target the light regulated circadian clock gene period 2. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41598-018-27080-2\">https://doi.org/10.1038/s41598-018-27080-2</a>","short":"R.M. Ceinos, E. Frigato, C. Pagano, N. Frohlich, P. Negrini, N. Cavallari, D. Vallone, S. Fuselli, C. Bertolucci, N.S. Foulkes, Scientific Reports 8 (2018).","ama":"Ceinos RM, Frigato E, Pagano C, et al. Mutations in blind cavefish target the light regulated circadian clock gene period 2. <i>Scientific Reports</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.1038/s41598-018-27080-2\">10.1038/s41598-018-27080-2</a>","ista":"Ceinos RM, Frigato E, Pagano C, Frohlich N, Negrini P, Cavallari N, Vallone D, Fuselli S, Bertolucci C, Foulkes NS. 2018. Mutations in blind cavefish target the light regulated circadian clock gene period 2. Scientific Reports. 8(1), 8754."},"year":"2018","date_created":"2018-12-11T11:45:36Z","abstract":[{"lang":"eng","text":"Light represents the principal signal driving circadian clock entrainment. However, how light influences the evolution of the clock remains poorly understood. The cavefish Phreatichthys andruzzii represents a fascinating model to explore how evolution under extreme aphotic conditions shapes the circadian clock, since in this species the clock is unresponsive to light. We have previously demonstrated that loss-of-function mutations targeting non-visual opsins contribute in part to this blind clock phenotype. Here, we have compared orthologs of two core clock genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii per2 transcript. The most abundant transcript encodes a truncated protein lacking the C-terminal Cry binding domain and incorporating an intronic, transposon-derived coding sequence. We demonstrate that the transposon insertion leads to a predominantly cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems that during evolution in complete darkness, the photic entrainment pathway of the circadian clock has been subject to mutation at multiple levels, extending from opsin photoreceptors to nuclear effectors."}],"publist_id":"7616","publication":"Scientific Reports","type":"journal_article","month":"06","intvolume":"         8","publication_status":"published","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","isi":1},{"file_date_updated":"2020-07-14T12:46:22Z","oa_version":"Published Version","volume":94,"title":"The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A","date_updated":"2024-10-09T20:58:42Z","author":[{"first_name":"Nicola","last_name":"Cavallari","full_name":"Cavallari, Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Nibau","full_name":"Nibau, Candida","first_name":"Candida"},{"first_name":"Armin","full_name":"Fuchs, Armin","last_name":"Fuchs"},{"first_name":"Despoina","full_name":"Dadarou, Despoina","last_name":"Dadarou"},{"full_name":"Barta, Andrea","last_name":"Barta","first_name":"Andrea"},{"full_name":"Doonan, John","last_name":"Doonan","first_name":"John"}],"ddc":["580"],"day":"01","doi":"10.1111/tpj.13914","scopus_import":"1","has_accepted_license":"1","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}],"file":[{"creator":"dernst","content_type":"application/pdf","access_level":"open_access","checksum":"d9d3ad3215ac0e581731443fca312266","file_id":"5934","file_name":"2018_PlantJourn_Cavallari.pdf","date_created":"2019-02-06T11:40:54Z","relation":"main_file","date_updated":"2020-07-14T12:46:22Z","file_size":1543354}],"status":"public","external_id":{"isi":["000434365500008"]},"acknowledgement":"CN, DD and JHD were funded by the BBSRC (grant number BB/M009459/1). NC was funded by the VIPS Program of the Austrian Federal Ministry of Science and Research and the City of Vienna. AB and AF were supported by the Austrian Science Fund (FWF) [DK W1207; SFB RNAreg F43-P10]","article_processing_charge":"No","issue":"6","oa":1,"publisher":"Wiley","corr_author":"1","_id":"403","date_published":"2018-06-01T00:00:00Z","publication_status":"published","intvolume":"        94","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","isi":1,"publication":"The Plant Journal","type":"journal_article","page":"1010 - 1022","quality_controlled":"1","citation":{"chicago":"Cavallari, Nicola, Candida Nibau, Armin Fuchs, Despoina Dadarou, Andrea Barta, and John Doonan. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF 65A.” <i>The Plant Journal</i>. Wiley, 2018. <a href=\"https://doi.org/10.1111/tpj.13914\">https://doi.org/10.1111/tpj.13914</a>.","ieee":"N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, and J. Doonan, “The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A,” <i>The Plant Journal</i>, vol. 94, no. 6. Wiley, pp. 1010–1022, 2018.","ista":"Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. 2018. The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. The Plant Journal. 94(6), 1010–1022.","apa":"Cavallari, N., Nibau, C., Fuchs, A., Dadarou, D., Barta, A., &#38; Doonan, J. (2018). The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. <i>The Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.13914\">https://doi.org/10.1111/tpj.13914</a>","mla":"Cavallari, Nicola, et al. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF 65A.” <i>The Plant Journal</i>, vol. 94, no. 6, Wiley, 2018, pp. 1010–22, doi:<a href=\"https://doi.org/10.1111/tpj.13914\">10.1111/tpj.13914</a>.","short":"N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, J. Doonan, The Plant Journal 94 (2018) 1010–1022.","ama":"Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. <i>The Plant Journal</i>. 2018;94(6):1010-1022. doi:<a href=\"https://doi.org/10.1111/tpj.13914\">10.1111/tpj.13914</a>"},"year":"2018","date_created":"2018-12-11T11:46:17Z","publist_id":"7426","abstract":[{"text":"The ability to adapt growth and development to temperature variations is crucial to generate plant varieties resilient to predicted temperature changes. However, the mechanisms underlying plant response to progressive increases in temperature have just started to be elucidated. Here, we report that the Cyclin-dependent Kinase G1 (CDKG1) is a central element in a thermo-sensitive mRNA splicing cascade that transduces changes in ambient temperature into differential expression of the fundamental spliceosome component, ATU2AF65A. CDKG1 is alternatively spliced in a temperature-dependent manner. We found that this process is partly dependent on both the Cyclin-dependent Kinase G2 (CDKG2) and the interacting co-factor CYCLIN L1 resulting in two distinct messenger RNAs. Relative abundance of both CDKG1 transcripts correlates with ambient temperature and possibly with different expression levels of the associated protein isoforms. Both CDKG1 alternative transcripts are necessary to fully complement the expression of ATU2AF65A across the temperature range. Our data support a previously unidentified temperature-dependent mechanism based on the alternative splicing of CDKG1 and regulated by CDKG2 and CYCLIN L1. We propose that changes in ambient temperature affect the relative abundance of CDKG1 transcripts and this in turn translates into differential CDKG1 protein expression coordinating the alternative splicing of ATU2AF65A. This article is protected by copyright. All rights reserved.","lang":"eng"}],"month":"06"},{"month":"06","citation":{"ista":"Kubiasová K, Mik V, Nisler J, Hönig M, Husičková A, Spíchal L, Pěkná Z, Šamajová O, Doležal K, Plíhal O, Benková E, Strnad M, Plíhalová L. 2018. Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. Phytochemistry. 150, 1–11.","ama":"Kubiasová K, Mik V, Nisler J, et al. Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. <i>Phytochemistry</i>. 2018;150:1-11. doi:<a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">10.1016/j.phytochem.2018.02.015</a>","mla":"Kubiasová, Karolina, et al. “Design, Synthesis and Perception of Fluorescently Labeled Isoprenoid Cytokinins.” <i>Phytochemistry</i>, vol. 150, Elsevier, 2018, pp. 1–11, doi:<a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">10.1016/j.phytochem.2018.02.015</a>.","short":"K. Kubiasová, V. Mik, J. Nisler, M. Hönig, A. Husičková, L. Spíchal, Z. Pěkná, O. Šamajová, K. Doležal, O. Plíhal, E. Benková, M. Strnad, L. Plíhalová, Phytochemistry 150 (2018) 1–11.","apa":"Kubiasová, K., Mik, V., Nisler, J., Hönig, M., Husičková, A., Spíchal, L., … Plíhalová, L. (2018). Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. <i>Phytochemistry</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">https://doi.org/10.1016/j.phytochem.2018.02.015</a>","chicago":"Kubiasová, Karolina, Václav Mik, Jaroslav Nisler, Martin Hönig, Alexandra Husičková, Lukáš Spíchal, Zuzana Pěkná, et al. “Design, Synthesis and Perception of Fluorescently Labeled Isoprenoid Cytokinins.” <i>Phytochemistry</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">https://doi.org/10.1016/j.phytochem.2018.02.015</a>.","ieee":"K. Kubiasová <i>et al.</i>, “Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins,” <i>Phytochemistry</i>, vol. 150. Elsevier, pp. 1–11, 2018."},"quality_controlled":"1","year":"2018","date_created":"2018-12-11T11:46:18Z","abstract":[{"lang":"eng","text":"Isoprenoid cytokinins play a number of crucial roles in the regulation of plant growth and development. To study cytokinin receptor properties in plants, we designed and prepared fluorescent derivatives of 6-[(3-methylbut-2-en-1-yl)amino]purine (N6-isopentenyladenine, iP) with several fluorescent labels attached to the C2 or N9 atom of the purine moiety via a 2- or 6-carbon linker. The fluorescent labels included dansyl (DS), fluorescein (FC), 7-nitrobenzofurazan (NBD), rhodamine B (RhoB), coumarin (Cou), 7-(diethylamino)coumarin (DEAC) and cyanine 5 dye (Cy5). All prepared compounds were screened for affinity for the Arabidopsis thaliana cytokinin receptor (CRE1/AHK4). Although the attachment of the fluorescent labels to iP via the linkers mostly disrupted binding to the receptor, several fluorescent derivatives interacted well. For this reason, three derivatives, two rhodamine B and one 4-chloro-7-nitrobenzofurazan labeled iP were tested for their interaction with CRE1/AHK4 and Zea mays cytokinin receptors in detail. We further showed that the three derivatives were able to activate transcription of cytokinin response regulator ARR5 in Arabidopsis seedlings. The activity of fluorescently labeled cytokinins was compared with corresponding 6-dimethylaminopurine fluorescently labeled negative controls. Selected rhodamine B C2-labeled compounds 17, 18 and 4-chloro-7-nitrobenzofurazan N9-labeled compound 28 and their respective negative controls (19, 20 and 29, respectively) were used for in planta staining experiments in Arabidopsis thaliana cell suspension culture using live cell confocal microscopy."}],"publist_id":"7422","type":"journal_article","publication":"Phytochemistry","page":"1-11","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","isi":1,"intvolume":"       150","publication_status":"published","_id":"407","date_published":"2018-06-01T00:00:00Z","article_processing_charge":"No","acknowledgement":"This work was supported by the Ministry of Education Youth and Sports, Czech Republic (grant LO1204 from the National Program of Sustainability I and Agricultural Research ) and by Czech Science Foundation grants 16-04184S , 501/10/1450 and 13-39982S and by IGA projects IGA_PrF_2018_033 and IGA_PrF_2018_023 . We would like to thank Jarmila Balonová, Olga Hustáková and Miroslava Šubová for their skillful technical assistance and Mgr. Tomáš Pospíšil, Ph.D. for his measurement of 1 H NMR and analysis of some 2D NMR spectral data. \r\n","publisher":"Elsevier","external_id":{"isi":["000435623400001"]},"status":"public","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}],"author":[{"full_name":"Kubiasová, Karolina","last_name":"Kubiasová","first_name":"Karolina"},{"last_name":"Mik","full_name":"Mik, Václav","first_name":"Václav"},{"last_name":"Nisler","full_name":"Nisler, Jaroslav","first_name":"Jaroslav"},{"full_name":"Hönig, Martin","last_name":"Hönig","first_name":"Martin"},{"full_name":"Husičková, Alexandra","last_name":"Husičková","first_name":"Alexandra"},{"first_name":"Lukáš","full_name":"Spíchal, Lukáš","last_name":"Spíchal"},{"first_name":"Zuzana","last_name":"Pěkná","full_name":"Pěkná, Zuzana"},{"last_name":"Šamajová","full_name":"Šamajová, Olga","first_name":"Olga"},{"full_name":"Doležal, Karel","last_name":"Doležal","first_name":"Karel"},{"full_name":"Plíhal, Ondřej","last_name":"Plíhal","first_name":"Ondřej"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Strnad, Miroslav","last_name":"Strnad","first_name":"Miroslav"},{"last_name":"Plíhalová","full_name":"Plíhalová, Lucie","first_name":"Lucie"}],"day":"01","doi":"10.1016/j.phytochem.2018.02.015","scopus_import":"1","title":"Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins","date_updated":"2023-09-11T12:53:11Z","oa_version":"None","volume":150},{"intvolume":"        69","publication_status":"published","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","isi":1,"citation":{"ieee":"M. Cucinotta, S. Manrique, C. Cuesta, E. Benková, O. Novák, and L. Colombo, “Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis,” <i>Journal of Experimental Botany</i>, vol. 69, no. 21. Oxford University Press, pp. 5169–5176, 2018.","chicago":"Cucinotta, Mara, Silvia Manrique, Candela Cuesta, Eva Benková, Ondřej Novák, and Lucia Colombo. “Cup-Shaped Cotyledon1 (CUC1) and CU2 Regulate Cytokinin Homeostasis to Determine Ovule Number in Arabidopsis.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2018. <a href=\"https://doi.org/10.1093/jxb/ery281\">https://doi.org/10.1093/jxb/ery281</a>.","mla":"Cucinotta, Mara, et al. “Cup-Shaped Cotyledon1 (CUC1) and CU2 Regulate Cytokinin Homeostasis to Determine Ovule Number in Arabidopsis.” <i>Journal of Experimental Botany</i>, vol. 69, no. 21, Oxford University Press, 2018, pp. 5169–76, doi:<a href=\"https://doi.org/10.1093/jxb/ery281\">10.1093/jxb/ery281</a>.","apa":"Cucinotta, M., Manrique, S., Cuesta, C., Benková, E., Novák, O., &#38; Colombo, L. (2018). Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/ery281\">https://doi.org/10.1093/jxb/ery281</a>","short":"M. Cucinotta, S. Manrique, C. Cuesta, E. Benková, O. Novák, L. Colombo, Journal of Experimental Botany 69 (2018) 5169–5176.","ama":"Cucinotta M, Manrique S, Cuesta C, Benková E, Novák O, Colombo L. Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis. <i>Journal of Experimental Botany</i>. 2018;69(21):5169-5176. doi:<a href=\"https://doi.org/10.1093/jxb/ery281\">10.1093/jxb/ery281</a>","ista":"Cucinotta M, Manrique S, Cuesta C, Benková E, Novák O, Colombo L. 2018. Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis. Journal of Experimental Botany. 69(21), 5169–5176."},"quality_controlled":"1","year":"2018","publist_id":"8012","abstract":[{"text":"Seeds derive from ovules upon fertilization and therefore the total number of ovules determines the final seed yield, a fundamental trait in crop plants. Among the factors that co-ordinate the process of ovule formation, the transcription factors CUP-SHAPED COTYLEDON 1 (CUC1) and CUC2 and the hormone cytokinin (CK) have a particularly prominent role. Indeed, the absence of both CUC1 and CUC2 causes a severe reduction in ovule number, a phenotype that can be rescued by CK treatment. In this study, we combined CK quantification with an integrative genome-wide target identification approach to select Arabidopsis genes regulated by CUCs that are also involved in CK metabolism. We focused our attention on the functional characterization of UDP-GLUCOSYL TRANSFERASE 85A3 (UGT85A3) and UGT73C1, which are up-regulated in the absence of CUC1 and CUC2 and encode enzymes able to catalyse CK inactivation by O-glucosylation. Our results demonstrate a role for these UGTs as a link between CUCs and CK homeostasis, and highlight the importance of CUCs and CKs in the determination of seed yield.","lang":"eng"}],"date_created":"2018-12-11T11:44:19Z","type":"journal_article","publication":"Journal of Experimental Botany","page":"5169 - 5176","month":"07","title":"Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis","date_updated":"2023-09-11T12:52:03Z","file_date_updated":"2020-07-14T12:46:25Z","volume":69,"oa_version":"Published Version","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}],"day":"26","ddc":["575"],"author":[{"last_name":"Cucinotta","full_name":"Cucinotta, Mara","first_name":"Mara"},{"first_name":"Silvia","full_name":"Manrique, Silvia","last_name":"Manrique"},{"first_name":"Candela","orcid":"0000-0003-1923-2410","last_name":"Cuesta","full_name":"Cuesta, Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739"},{"first_name":"Ondřej","full_name":"Novák, Ondřej","last_name":"Novák"},{"full_name":"Colombo, Lucia","last_name":"Colombo","first_name":"Lucia"}],"has_accepted_license":"1","doi":"10.1093/jxb/ery281","scopus_import":"1","issue":"21","oa":1,"article_processing_charge":"No","acknowledgement":"This work was funded by the Ministry of Education, Youth and Sports of the Czech Republic through the National Program of Sustainability (grant no. LO1204).","publisher":"Oxford University Press","status":"public","file":[{"checksum":"ca3b6711040b1662488aeb3d1f961f13","creator":"dernst","content_type":"application/pdf","access_level":"open_access","file_size":1292128,"file_name":"2018_JournalExperimBotany_Cucinotta.pdf","date_updated":"2020-07-14T12:46:25Z","relation":"main_file","date_created":"2018-12-17T10:44:16Z","file_id":"5691"}],"external_id":{"isi":["000448163900015"]},"_id":"42","date_published":"2018-07-26T00:00:00Z"},{"scopus_import":"1","doi":"10.1016/bs.abr.2018.09.007","author":[{"last_name":"Abualia","full_name":"Abualia, Rashed","id":"4827E134-F248-11E8-B48F-1D18A9856A87","first_name":"Rashed","orcid":"0000-0002-9357-9415"},{"last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739"},{"full_name":"Lacombe, Benoît","last_name":"Lacombe","first_name":"Benoît"}],"day":"01","language":[{"iso":"eng"}],"related_material":{"record":[{"relation":"dissertation_contains","id":"10303","status":"public"}]},"department":[{"_id":"EvBe"}],"volume":87,"oa_version":"None","date_updated":"2026-09-01T22:30:53Z","title":"Transporters and mechanisms of hormone transport in arabidopsis","date_published":"2018-01-01T00:00:00Z","_id":"47","external_id":{"isi":["000453657800006"]},"status":"public","publisher":"Elsevier","article_processing_charge":"No","isi":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_status":"published","intvolume":"        87","month":"01","page":"115 - 138","type":"journal_article","publication":"Advances in Botanical Research","date_created":"2018-12-11T11:44:20Z","abstract":[{"text":"Plant hormones as signalling molecules play an essential role in the control of plant growth and development. Typically, sites of hormonal action are usually distant from the site of biosynthesis thus relying on efficient transport mechanisms. Over the last decades, molecular identification of proteins and protein complexes involved in hormonal transport has started. Advanced screens for genes involved in hormonal transport in combination with transport assays using heterologous systems such as yeast, insect, or tobacco BY2 cells or Xenopus oocytes provided important insights into mechanisms underlying distribution of hormones in plant body and led to identification of principal transporters for each hormone. This review gives a short overview of the mechanisms of hormonal transport and transporters identified in Arabidopsis thaliana.","lang":"eng"}],"publist_id":"8007","year":"2018","quality_controlled":"1","citation":{"ieee":"R. Abualia, E. Benková, and B. Lacombe, “Transporters and mechanisms of hormone transport in arabidopsis,” <i>Advances in Botanical Research</i>, vol. 87. Elsevier, pp. 115–138, 2018.","chicago":"Abualia, Rashed, Eva Benková, and Benoît Lacombe. “Transporters and Mechanisms of Hormone Transport in Arabidopsis.” <i>Advances in Botanical Research</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">https://doi.org/10.1016/bs.abr.2018.09.007</a>.","apa":"Abualia, R., Benková, E., &#38; Lacombe, B. (2018). Transporters and mechanisms of hormone transport in arabidopsis. <i>Advances in Botanical Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">https://doi.org/10.1016/bs.abr.2018.09.007</a>","short":"R. Abualia, E. Benková, B. Lacombe, Advances in Botanical Research 87 (2018) 115–138.","mla":"Abualia, Rashed, et al. “Transporters and Mechanisms of Hormone Transport in Arabidopsis.” <i>Advances in Botanical Research</i>, vol. 87, Elsevier, 2018, pp. 115–38, doi:<a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">10.1016/bs.abr.2018.09.007</a>.","ama":"Abualia R, Benková E, Lacombe B. Transporters and mechanisms of hormone transport in arabidopsis. <i>Advances in Botanical Research</i>. 2018;87:115-138. doi:<a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">10.1016/bs.abr.2018.09.007</a>","ista":"Abualia R, Benková E, Lacombe B. 2018. Transporters and mechanisms of hormone transport in arabidopsis. Advances in Botanical Research. 87, 115–138."}},{"date_created":"2018-12-11T11:45:06Z","abstract":[{"text":"Intercellular distribution of the plant hormone auxin largely depends on the polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters. PIN polarity switches in response to different developmental and environmental signals have been shown to redirect auxin fluxes mediating certain developmental responses. PIN phosphorylation at different sites and by different kinases is crucial for PIN function. Here we investigate the role of PIN phosphorylation during gravitropic response. Loss- and gain-of-function mutants in PINOID and related kinases but not in D6PK kinase as well as mutations mimicking constitutive dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements in response to gravity and during feed-back regulation by auxin itself. Thus PIN phosphorylation, besides regulating transport activity and apical-basal targeting, is also important for the rapid polarity switches in response to environmental and endogenous signals.","lang":"eng"}],"publist_id":"7729","year":"2018","quality_controlled":"1","citation":{"ieee":"P. Grones <i>et al.</i>, “PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism,” <i>Scientific Reports</i>, vol. 8, no. 1. Springer, 2018.","chicago":"Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann, Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific Reports</i>. Springer, 2018. <a href=\"https://doi.org/10.1038/s41598-018-28188-1\">https://doi.org/10.1038/s41598-018-28188-1</a>.","ama":"Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. <i>Scientific Reports</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.1038/s41598-018-28188-1\">10.1038/s41598-018-28188-1</a>","short":"P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J. Friml, Scientific Reports 8 (2018).","mla":"Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific Reports</i>, vol. 8, no. 1, 10279, Springer, 2018, doi:<a href=\"https://doi.org/10.1038/s41598-018-28188-1\">10.1038/s41598-018-28188-1</a>.","apa":"Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J., &#38; Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. <i>Scientific Reports</i>. Springer. <a href=\"https://doi.org/10.1038/s41598-018-28188-1\">https://doi.org/10.1038/s41598-018-28188-1</a>","ista":"Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. Scientific Reports. 8(1), 10279."},"type":"journal_article","publication":"Scientific Reports","month":"07","intvolume":"         8","publication_status":"published","isi":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Springer","article_processing_charge":"No","issue":"1","oa":1,"external_id":{"isi":["000437673200053"]},"status":"public","file":[{"access_level":"open_access","content_type":"application/pdf","creator":"dernst","checksum":"266b03f4fb8198e83141617aaa99dcab","relation":"main_file","date_updated":"2020-07-14T12:45:20Z","date_created":"2018-12-17T15:38:56Z","file_name":"2018_ScientificReports_Grones.pdf","file_id":"5714","file_size":2413876}],"date_published":"2018-07-06T00:00:00Z","_id":"191","date_updated":"2026-09-01T22:30:58Z","title":"PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism","project":[{"call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300"},{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"ec_funded":1,"volume":8,"oa_version":"Published Version","article_number":"10279","file_date_updated":"2020-07-14T12:45:20Z","language":[{"iso":"eng"}],"related_material":{"record":[{"relation":"dissertation_contains","id":"8822","status":"public"}]},"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"scopus_import":"1","doi":"10.1038/s41598-018-28188-1","has_accepted_license":"1","author":[{"first_name":"Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87","last_name":"Grones","full_name":"Grones, Peter"},{"first_name":"Melinda F","full_name":"Abas, Melinda F","last_name":"Abas","id":"3CFB3B1C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jakub","orcid":"0000-0003-2140-7195","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","full_name":"Hajny, Jakub","last_name":"Hajny"},{"full_name":"Jones, Angharad","last_name":"Jones","first_name":"Angharad"},{"first_name":"Sascha","full_name":"Waidmann, Sascha","last_name":"Waidmann"},{"full_name":"Kleine Vehn, Jürgen","last_name":"Kleine Vehn","first_name":"Jürgen"},{"full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí"}],"day":"06","ddc":["581"]},{"date_updated":"2026-07-29T13:30:01Z","title":"Identification and characterization of novel auxin-cytokinin cross-talk components","file_date_updated":"2020-12-02T23:30:08Z","oa_version":"Published Version","related_material":{"record":[{"status":"public","id":"1024","relation":"part_of_dissertation"}]},"department":[{"_id":"EvBe"},{"_id":"GradSch"}],"language":[{"iso":"eng"}],"author":[{"id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87","full_name":"Hurny, Andrej","last_name":"Hurny","orcid":"0000-0003-3638-1426","first_name":"Andrej"}],"day":"01","ddc":["570"],"doi":"10.15479/AT:ISTA:th_930","has_accepted_license":"1","article_processing_charge":"No","oa":1,"publisher":"Institute of Science and Technology Austria","status":"public","file":[{"embargo_to":"open_access","checksum":"0c9d6d1c80d9857e6e545213467bbcb2","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","access_level":"closed","creator":"dernst","file_size":28112114,"date_updated":"2020-12-02T23:30:08Z","relation":"source_file","date_created":"2019-04-05T09:37:56Z","file_name":"2018_Hurny_thesis_source.docx","file_id":"6226"},{"access_level":"open_access","content_type":"application/pdf","creator":"dernst","checksum":"ecbe481a1413d270bd501b872c7ed54f","embargo":"2019-07-10","file_id":"6227","date_updated":"2020-12-02T09:52:16Z","date_created":"2019-04-05T09:37:55Z","relation":"main_file","file_name":"2018_Hurny_thesis.pdf","file_size":12524427}],"_id":"539","date_published":"2018-01-01T00:00:00Z","corr_author":"1","alternative_title":["ISTA Thesis"],"doi_confirm":"1","publication_identifier":{"issn":["2663-337X"]},"publication_status":"published","supervisor":[{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","first_name":"Eva"}],"pubrep_id":"930","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","year":"2018","citation":{"mla":"Hurny, Andrej. <i>Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components</i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">10.15479/AT:ISTA:th_930</a>.","short":"A. Hurny, Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components, Institute of Science and Technology Austria, 2018.","apa":"Hurny, A. (2018). <i>Identification and characterization of novel auxin-cytokinin cross-talk components</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">https://doi.org/10.15479/AT:ISTA:th_930</a>","ama":"Hurny A. Identification and characterization of novel auxin-cytokinin cross-talk components. 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">10.15479/AT:ISTA:th_930</a>","ista":"Hurny A. 2018. Identification and characterization of novel auxin-cytokinin cross-talk components. Institute of Science and Technology Austria.","ieee":"A. Hurny, “Identification and characterization of novel auxin-cytokinin cross-talk components,” Institute of Science and Technology Austria, 2018.","chicago":"Hurny, Andrej. “Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components.” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">https://doi.org/10.15479/AT:ISTA:th_930</a>."},"date_created":"2018-12-11T11:47:03Z","abstract":[{"text":"The whole life cycle of plants as well as their responses to environmental stimuli is governed by a complex network of hormonal regulations. A number of studies have demonstrated an essential role of both auxin and cytokinin in the regulation of many aspects of plant growth and development including embryogenesis, postembryonic organogenic processes such as root, and shoot branching, root and shoot apical meristem activity and phyllotaxis. Over the last decades essential knowledge on the key molecular factors and pathways that spatio-temporally define auxin and cytokinin activities in the plant body has accumulated. However, how both hormonal pathways are interconnected by a complex network of interactions and feedback circuits that determines the final outcome of the individual hormone actions is still largely unknown. Root system architecture establishment and in particular formation of lateral organs is prime example of developmental process at whose regulation both auxin and cytokinin pathways converge. To dissect convergence points and pathways that tightly balance auxin - cytokinin antagonistic activities that determine the root branching pattern transcriptome profiling was applied. Genome wide expression analyses of the xylem pole pericycle, a tissue giving rise to lateral roots, led to identification of genes that are highly responsive to combinatorial auxin and cytokinin treatments and play an essential function in the auxin-cytokinin regulated root branching. SYNERGISTIC AUXIN CYTOKININ 1 (SYAC1) gene, which encodes for a protein of unknown function, was detected among the top candidate genes of which expression was synergistically up-regulated by simultaneous hormonal treatment. Plants with modulated SYAC1 activity exhibit severe defects in the root system establishment and attenuate developmental responses to both auxin and cytokinin. To explore the biological function of the SYAC1, we employed different strategies including expression pattern analysis, subcellular localization and phenotypic analyses of the syac1 loss-of-function and gain-of-function transgenic lines along with the identification of the SYAC1 interaction partners. Detailed functional characterization revealed that SYAC1 acts as a developmentally specific regulator of the secretory pathway to control deposition of cell wall components and thereby rapidly fine tune elongation growth.","lang":"eng"}],"publist_id":"7277","type":"dissertation","degree_awarded":"PhD","page":"147","OA_place":"publisher","month":"01"},{"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"isi":1,"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","pubrep_id":"1017","publication_status":"published","publication_identifier":{"issn":["0959-437X"]},"intvolume":"        45","month":"08","type":"journal_article","publication":"Current Opinion in Genetics & Development","page":"82 - 89","year":"2017","citation":{"ista":"Ötvös K, Benková E. 2017. Spatiotemporal mechanisms of root branching. Current Opinion in Genetics &#38; Development. 45, 82–89.","short":"K. Ötvös, E. Benková, Current Opinion in Genetics &#38; Development 45 (2017) 82–89.","apa":"Ötvös, K., &#38; Benková, E. (2017). Spatiotemporal mechanisms of root branching. <i>Current Opinion in Genetics &#38; Development</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">https://doi.org/10.1016/j.gde.2017.03.010</a>","mla":"Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.” <i>Current Opinion in Genetics &#38; Development</i>, vol. 45, Elsevier, 2017, pp. 82–89, doi:<a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">10.1016/j.gde.2017.03.010</a>.","ama":"Ötvös K, Benková E. Spatiotemporal mechanisms of root branching. <i>Current Opinion in Genetics &#38; Development</i>. 2017;45:82-89. doi:<a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">10.1016/j.gde.2017.03.010</a>","chicago":"Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.” <i>Current Opinion in Genetics &#38; Development</i>. Elsevier, 2017. <a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">https://doi.org/10.1016/j.gde.2017.03.010</a>.","ieee":"K. Ötvös and E. Benková, “Spatiotemporal mechanisms of root branching,” <i>Current Opinion in Genetics &#38; Development</i>, vol. 45. Elsevier, pp. 82–89, 2017."},"quality_controlled":"1","publist_id":"6394","abstract":[{"text":"The fundamental tasks of the root system are, besides anchoring, mediating interactions between plant and soil and providing the plant with water and nutrients. The architecture of the root system is controlled by endogenous mechanisms that constantly integrate environmental signals, such as availability of nutrients and water. Extremely important for efficient soil exploitation and survival under less favorable conditions is the developmental flexibility of the root system that is largely determined by its postembryonic branching capacity. Modulation of initiation and outgrowth of lateral roots provides roots with an exceptional plasticity, allows optimal adjustment to underground heterogeneity, and enables effective soil exploitation and use of resources. Here we discuss recent advances in understanding the molecular mechanisms that shape the plant root system and integrate external cues to adapt to the changing environment.","lang":"eng"}],"date_created":"2018-12-11T11:49:38Z","ddc":["575"],"day":"01","author":[{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","full_name":"Ötvös, Krisztina","last_name":"Ötvös","orcid":"0000-0002-5503-4983","first_name":"Krisztina"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková"}],"has_accepted_license":"1","doi":"10.1016/j.gde.2017.03.010","scopus_import":"1","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}],"file_date_updated":"2019-04-17T08:00:36Z","volume":45,"oa_version":"Submitted Version","project":[{"call_identifier":"FWF","_id":"2542D156-B435-11E9-9278-68D0E5697425","grant_number":"I 1774-B16","name":"Hormone cross-talk drives nutrient dependent plant development"}],"date_updated":"2026-04-16T09:56:36Z","title":"Spatiotemporal mechanisms of root branching","_id":"1004","date_published":"2017-08-01T00:00:00Z","pmid":1,"file":[{"file_id":"6336","file_name":"Otvos_Benkova_CurOpDevBiol_2017.pdf","date_updated":"2019-04-17T08:00:36Z","relation":"main_file","date_created":"2019-04-17T08:00:36Z","file_size":364133,"success":1,"creator":"dernst","content_type":"application/pdf","access_level":"open_access"}],"status":"public","external_id":{"isi":["000404880400013"],"pmid":["28391060"]},"oa":1,"article_processing_charge":"No","publisher":"Elsevier"},{"month":"05","page":"387 - 404","type":"journal_article","publication":"Plant Physiology","abstract":[{"lang":"eng","text":"In plants, the multistep phosphorelay (MSP) pathway mediates a range of regulatory processes, including those activated by cytokinins. The crosstalk between cytokinin response and light is known for a long time. However, the molecular mechanism underlying the interactionbetween light and cytokinin signaling remains elusive. In the screen for upstream regulators we identified a LONG PALE HYPOCOTYL (LPH) gene whose activity is indispensable for spatiotemporally correct expression of CYTOKININ INDEPENDENT-1 (CKI1), encoding the constitutively active sensor histidine kinase that activates MSP signaling. lph is a new allele of HEME OXYGENASE 1 (HY1) which encodes the key protein in the biosynthesis of phytochromobilin, a cofactor of photoconvertiblephytochromes. Our analysis confirmed the light-dependent regulation oftheCKI1 expression pattern. We show that CKI1 expression is under the control of phytochrome A (phyA), functioning as a dual (both positive and negative) regulator of CKI1 expression, presumably via the phyA-regulated transcription factors PHYTOCHROME INTERACTING FACTOR 3 (PIF3) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1). Changes in CKI1 expression observed in lph/hy1-7 and phy mutants correlatewithmisregulation of MSP signaling, changedcytokinin sensitivity and developmental aberrations,previously shown to be associated with cytokinin and/or CKI1 action. Besides that, we demonstrate novel role of phyA-dependent CKI1 expression in the hypocotyl elongation and hook development during skotomorphogenesis. Based on these results, we propose that the light-dependent regulation of CKI1 provides a plausible mechanistic link underlying the well-known interaction between light- and cytokinin-controlled plant development."}],"publist_id":"6375","date_created":"2018-12-11T11:49:43Z","quality_controlled":"1","year":"2017","citation":{"ieee":"T. Dobisova <i>et al.</i>, “Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development,” <i>Plant Physiology</i>, vol. 174, no. 1. American Society of Plant Biologists, pp. 387–404, 2017.","chicago":"Dobisova, Tereza, Vendula Hrdinova, Candela Cuesta, Sarka Michlickova, Ivana Urbankova, Romana Hejatkova, Petra Zadnikova, Markéta Pernisová, Eva Benková, and Jan Hejátko. “Light Regulated Expression of Sensor Histidine Kinase CKI1 Controls Cytokinin Related Development.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2017. <a href=\"https://doi.org/10.1104/pp.16.01964\">https://doi.org/10.1104/pp.16.01964</a>.","ama":"Dobisova T, Hrdinova V, Cuesta C, et al. Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development. <i>Plant Physiology</i>. 2017;174(1):387-404. doi:<a href=\"https://doi.org/10.1104/pp.16.01964\">10.1104/pp.16.01964</a>","mla":"Dobisova, Tereza, et al. “Light Regulated Expression of Sensor Histidine Kinase CKI1 Controls Cytokinin Related Development.” <i>Plant Physiology</i>, vol. 174, no. 1, American Society of Plant Biologists, 2017, pp. 387–404, doi:<a href=\"https://doi.org/10.1104/pp.16.01964\">10.1104/pp.16.01964</a>.","short":"T. Dobisova, V. Hrdinova, C. Cuesta, S. Michlickova, I. Urbankova, R. Hejatkova, P. Zadnikova, M. Pernisová, E. Benková, J. Hejátko, Plant Physiology 174 (2017) 387–404.","apa":"Dobisova, T., Hrdinova, V., Cuesta, C., Michlickova, S., Urbankova, I., Hejatkova, R., … Hejátko, J. (2017). Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.16.01964\">https://doi.org/10.1104/pp.16.01964</a>","ista":"Dobisova T, Hrdinova V, Cuesta C, Michlickova S, Urbankova I, Hejatkova R, Zadnikova P, Pernisová M, Benková E, Hejátko J. 2017. Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development. Plant Physiology. 174(1), 387–404."},"isi":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_status":"published","intvolume":"       174","date_published":"2017-05-17T00:00:00Z","_id":"1018","external_id":{"isi":["000402057200028"]},"status":"public","publisher":"American Society of Plant Biologists","issue":"1","article_processing_charge":"No","scopus_import":"1","doi":"10.1104/pp.16.01964","day":"17","author":[{"first_name":"Tereza","last_name":"Dobisova","full_name":"Dobisova, Tereza"},{"first_name":"Vendula","full_name":"Hrdinova, Vendula","last_name":"Hrdinova"},{"id":"33A3C818-F248-11E8-B48F-1D18A9856A87","last_name":"Cuesta","full_name":"Cuesta, Candela","orcid":"0000-0003-1923-2410","first_name":"Candela"},{"first_name":"Sarka","full_name":"Michlickova, Sarka","last_name":"Michlickova"},{"first_name":"Ivana","full_name":"Urbankova, Ivana","last_name":"Urbankova"},{"first_name":"Romana","last_name":"Hejatkova","full_name":"Hejatkova, Romana"},{"first_name":"Petra","last_name":"Zadnikova","full_name":"Zadnikova, Petra"},{"first_name":"Markéta","last_name":"Pernisová","full_name":"Pernisová, Markéta"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva"},{"first_name":"Jan","full_name":"Hejátko, Jan","last_name":"Hejátko"}],"language":[{"iso":"eng"}],"department":[{"_id":"EvBe"}],"oa_version":"None","volume":174,"title":"Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development","date_updated":"2023-09-22T09:41:48Z"},{"_id":"946","date_published":"2017-06-19T00:00:00Z","acknowledgement":"Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013 no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop at IST Austria for their contribution to the microscope setup and to Yvonne Kemper for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility","article_processing_charge":"Yes","oa":1,"publisher":"eLife Sciences Publications","file":[{"file_id":"5315","relation":"main_file","date_created":"2018-12-12T10:17:57Z","date_updated":"2020-07-14T12:48:15Z","file_name":"IST-2017-847-v1+1_elife-26792-v2.pdf","file_size":19581847,"content_type":"application/pdf","access_level":"open_access","creator":"system","checksum":"9af3398cb0d81f99d79016a616df22e9"}],"external_id":{"isi":["000404728300001"]},"status":"public","related_material":{"record":[{"relation":"popular_science","id":"5566","status":"public"}]},"department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EvBe"}],"language":[{"iso":"eng"}],"author":[{"last_name":"Von Wangenheim","full_name":"Von Wangenheim, Daniel","id":"49E91952-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6862-1247","first_name":"Daniel"},{"first_name":"Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-9767-8699","first_name":"Matyas","full_name":"Fendrych, Matyas","last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Vanessa","orcid":"0000-0003-2676-3367","last_name":"Barone","full_name":"Barone, Vanessa","id":"419EECCC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jirí"}],"day":"19","ddc":["570"],"doi":"10.7554/eLife.26792","scopus_import":"1","has_accepted_license":"1","ec_funded":1,"project":[{"call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"},{"call_identifier":"FWF","grant_number":"M02128","_id":"2572ED28-B435-11E9-9278-68D0E5697425","name":"Molecular basis of root growth inhibition by auxin"},{"name":"Hormone cross-talk drives nutrient dependent plant development","_id":"2542D156-B435-11E9-9278-68D0E5697425","grant_number":"I 1774-B16","call_identifier":"FWF"},{"grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7"}],"date_updated":"2025-04-15T06:37:26Z","title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","file_date_updated":"2020-07-14T12:48:15Z","volume":6,"oa_version":"Published Version","article_number":"e26792","month":"06","year":"2017","citation":{"ieee":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J. Friml, “Live tracking of moving samples in confocal microscopy for vertically grown roots,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017.","chicago":"Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone, Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>.","ama":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>","short":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml, ELife 6 (2017).","mla":"von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>, vol. 6, e26792, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>.","apa":"von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &#38; Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>","ista":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 6, e26792."},"quality_controlled":"1","date_created":"2018-12-11T11:49:21Z","publist_id":"6471","abstract":[{"text":"Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies. Live imaging greatly aids these efforts, but the horizontal sample position and continuous root tip displacement present significant difficulties. Here, we develop a confocal microscope setup for vertical sample mounting and integrated directional illumination. We present TipTracker – a custom software for automatic tracking of diverse moving objects usable on various microscope setups. Combined, this enables observation of root tips growing along the natural gravity vector over prolonged periods of time, as well as the ability to induce rapid gravity or light stimulation. We also track migrating cells in the developing zebrafish embryo, demonstrating the utility of this system in the acquisition of high-resolution data sets of dynamic samples. We provide detailed descriptions of the tools enabling the easy implementation on other microscopes.","lang":"eng"}],"type":"journal_article","publication":"eLife","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"pubrep_id":"847","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","isi":1,"intvolume":"         6","publication_status":"published"},{"status":"public","file":[{"file_size":840646,"file_id":"5068","file_name":"IST-2018-1019-v1+1_Hurny_MethodsMolBiol_2017.pdf","relation":"main_file","date_created":"2018-12-12T10:14:18Z","date_updated":"2019-10-15T07:47:05Z","creator":"system","access_level":"open_access","content_type":"application/pdf"}],"publisher":"Springer","article_processing_charge":"No","oa":1,"corr_author":"1","date_published":"2017-03-17T00:00:00Z","_id":"1024","oa_version":"Submitted Version","volume":1569,"file_date_updated":"2019-10-15T07:47:05Z","title":"Methodological advances in auxin and cytokinin biology","date_updated":"2026-09-01T22:31:14Z","project":[{"name":"Hormone cross-talk drives nutrient dependent plant development","grant_number":"I 1774-B16","_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"doi":"10.1007/978-1-4939-6831-2_1","scopus_import":"1","has_accepted_license":"1","author":[{"first_name":"Andrej","orcid":"0000-0003-3638-1426","full_name":"Hurny, Andrej","last_name":"Hurny","id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva"}],"day":"17","ddc":["575"],"language":[{"iso":"eng"}],"related_material":{"record":[{"id":"539","status":"public","relation":"dissertation_contains"}]},"department":[{"_id":"EvBe"}],"page":"1 - 29","type":"journal_article","publication":"Auxins and Cytokinins in Plant Biology","date_created":"2018-12-11T11:49:45Z","abstract":[{"text":"The history of auxin and cytokinin biology including the initial discoveries by father–son duo Charles Darwin and Francis Darwin (1880), and Gottlieb Haberlandt (1919) is a beautiful demonstration of unceasing continuity of research. Novel findings are integrated into existing hypotheses and models and deepen our understanding of biological principles. At the same time new questions are triggered and hand to hand with this new methodologies are developed to address these new challenges.","lang":"eng"}],"publist_id":"6369","quality_controlled":"1","year":"2017","citation":{"chicago":"Hurny, Andrej, and Eva Benková. “Methodological Advances in Auxin and Cytokinin Biology.” <i>Auxins and Cytokinins in Plant Biology</i>. Springer, 2017. <a href=\"https://doi.org/10.1007/978-1-4939-6831-2_1\">https://doi.org/10.1007/978-1-4939-6831-2_1</a>.","ieee":"A. Hurny and E. Benková, “Methodological advances in auxin and cytokinin biology,” <i>Auxins and Cytokinins in Plant Biology</i>, vol. 1569. Springer, pp. 1–29, 2017.","ista":"Hurny A, Benková E. 2017. Methodological advances in auxin and cytokinin biology. Auxins and Cytokinins in Plant Biology. 1569, 1–29.","ama":"Hurny A, Benková E. Methodological advances in auxin and cytokinin biology. <i>Auxins and Cytokinins in Plant Biology</i>. 2017;1569:1-29. doi:<a href=\"https://doi.org/10.1007/978-1-4939-6831-2_1\">10.1007/978-1-4939-6831-2_1</a>","apa":"Hurny, A., &#38; Benková, E. (2017). Methodological advances in auxin and cytokinin biology. <i>Auxins and Cytokinins in Plant Biology</i>. Springer. <a href=\"https://doi.org/10.1007/978-1-4939-6831-2_1\">https://doi.org/10.1007/978-1-4939-6831-2_1</a>","mla":"Hurny, Andrej, and Eva Benková. “Methodological Advances in Auxin and Cytokinin Biology.” <i>Auxins and Cytokinins in Plant Biology</i>, vol. 1569, Springer, 2017, pp. 1–29, doi:<a href=\"https://doi.org/10.1007/978-1-4939-6831-2_1\">10.1007/978-1-4939-6831-2_1</a>.","short":"A. Hurny, E. Benková, Auxins and Cytokinins in Plant Biology 1569 (2017) 1–29."},"month":"03","publication_identifier":{"issn":["1064-3745"]},"publication_status":"published","alternative_title":["Methods in Molecular Biology"],"intvolume":"      1569","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pubrep_id":"1019"},{"external_id":{"isi":["000390135400013"]},"status":"public","publisher":"American Society of Plant Biologists","article_processing_charge":"No","acknowledgement":"We thank Martine De Cock and Annick Bleys for help in preparing the manuscript, Daniel Van Damme for sharing material and stimulating discussion, and Rudiger Simon for support during revision of the manuscript.\r\nThis work was supported by grants from the European Research Council (StartingIndependentResearchGrantERC-2007-Stg-207362-HCPO)and the Czech Science Foundation (GACR CZ.1.07/2.3.00/20.0043) to E.B.\r\nand Natural Sciences and Engineering Research Council of Canada Discovery Grant 2014-05325 to P.P. K.W. acknowledges funding from a Human Frontier Science Program Long-Term Fellowship (LT-000209-2014).","issue":"10","oa":1,"corr_author":"1","date_published":"2016-10-01T00:00:00Z","_id":"1153","volume":28,"oa_version":"Submitted Version","title":"A model of differential growth guided apical hook formation in plants","date_updated":"2025-09-22T09:56:45Z","ec_funded":1,"project":[{"call_identifier":"FP7","grant_number":"207362","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","name":"Hormonal cross-talk in plant organogenesis"}],"doi":"10.1105/tpc.15.00569","scopus_import":"1","author":[{"first_name":"Petra","full_name":"Žádníková, Petra","last_name":"Žádníková"},{"last_name":"Wabnik","full_name":"Wabnik, Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7263-0560","first_name":"Krzysztof T"},{"first_name":"Anas","last_name":"Abuzeineh","full_name":"Abuzeineh, Anas"},{"last_name":"Gallemí","full_name":"Gallemí, Marçal","first_name":"Marçal"},{"first_name":"Dominique","full_name":"Van Der Straeten, Dominique","last_name":"Van Der Straeten"},{"first_name":"Richard","last_name":"Smith","full_name":"Smith, Richard"},{"last_name":"Inze","full_name":"Inze, Dirk","first_name":"Dirk"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí"},{"last_name":"Prusinkiewicz","full_name":"Prusinkiewicz, Przemysław","first_name":"Przemysław"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková"}],"day":"01","language":[{"iso":"eng"}],"department":[{"_id":"EvBe"},{"_id":"JiFr"}],"page":"2464 - 2477","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134968/"}],"publication":"Plant Cell","type":"journal_article","date_created":"2018-12-11T11:50:26Z","abstract":[{"lang":"eng","text":"Differential cell growth enables flexible organ bending in the presence of environmental signals such as light or gravity. A prominent example of the developmental processes based on differential cell growth is the formation of the apical hook that protects the fragile shoot apical meristem when it breaks through the soil during germination. Here, we combined in silico and in vivo approaches to identify a minimal mechanism producing auxin gradient-guided differential growth during the establishment of the apical hook in the model plant Arabidopsis thaliana. Computer simulation models based on experimental data demonstrate that asymmetric expression of the PIN-FORMED auxin efflux carrier at the concave (inner) versus convex (outer) side of the hook suffices to establish an auxin maximum in the epidermis at the concave side of the apical hook. Furthermore, we propose a mechanism that translates this maximum into differential growth, and thus curvature, of the apical hook. Through a combination of experimental and in silico computational approaches, we have identified the individual contributions of differential cell elongation and proliferation to defining the apical hook and reveal the role of auxin-ethylene crosstalk in balancing these two processes. © 2016 American Society of Plant Biologists. All rights reserved."}],"publist_id":"6205","quality_controlled":"1","year":"2016","citation":{"ista":"Žádníková P, Wabnik KT, Abuzeineh A, Gallemí M, Van Der Straeten D, Smith R, Inze D, Friml J, Prusinkiewicz P, Benková E. 2016. A model of differential growth guided apical hook formation in plants. Plant Cell. 28(10), 2464–2477.","ama":"Žádníková P, Wabnik KT, Abuzeineh A, et al. A model of differential growth guided apical hook formation in plants. <i>Plant Cell</i>. 2016;28(10):2464-2477. doi:<a href=\"https://doi.org/10.1105/tpc.15.00569\">10.1105/tpc.15.00569</a>","apa":"Žádníková, P., Wabnik, K. T., Abuzeineh, A., Gallemí, M., Van Der Straeten, D., Smith, R., … Benková, E. (2016). A model of differential growth guided apical hook formation in plants. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.15.00569\">https://doi.org/10.1105/tpc.15.00569</a>","short":"P. Žádníková, K.T. Wabnik, A. Abuzeineh, M. Gallemí, D. Van Der Straeten, R. Smith, D. Inze, J. Friml, P. Prusinkiewicz, E. Benková, Plant Cell 28 (2016) 2464–2477.","mla":"Žádníková, Petra, et al. “A Model of Differential Growth Guided Apical Hook Formation in Plants.” <i>Plant Cell</i>, vol. 28, no. 10, American Society of Plant Biologists, 2016, pp. 2464–77, doi:<a href=\"https://doi.org/10.1105/tpc.15.00569\">10.1105/tpc.15.00569</a>.","chicago":"Žádníková, Petra, Krzysztof T Wabnik, Anas Abuzeineh, Marçal Gallemí, Dominique Van Der Straeten, Richard Smith, Dirk Inze, Jiří Friml, Przemysław Prusinkiewicz, and Eva Benková. “A Model of Differential Growth Guided Apical Hook Formation in Plants.” <i>Plant Cell</i>. American Society of Plant Biologists, 2016. <a href=\"https://doi.org/10.1105/tpc.15.00569\">https://doi.org/10.1105/tpc.15.00569</a>.","ieee":"P. Žádníková <i>et al.</i>, “A model of differential growth guided apical hook formation in plants,” <i>Plant Cell</i>, vol. 28, no. 10. American Society of Plant Biologists, pp. 2464–2477, 2016."},"month":"10","publication_status":"published","intvolume":"        28","isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"publication_status":"published","intvolume":"       143","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"type":"journal_article","publication":"Development","page":"4419 - 4424","citation":{"ieee":"M. Cucinotta <i>et al.</i>, “Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development,” <i>Development</i>, vol. 143, no. 23. Company of Biologists, pp. 4419–4424, 2016.","chicago":"Cucinotta, Mara, Silvia Manrique, Andrea Guazzotti, Nadia Quadrelli, Marta Mendes, Eva Benková, and Lucia Colombo. “Cytokinin Response Factors Integrate Auxin and Cytokinin Pathways for Female Reproductive Organ Development.” <i>Development</i>. Company of Biologists, 2016. <a href=\"https://doi.org/10.1242/dev.143545\">https://doi.org/10.1242/dev.143545</a>.","ama":"Cucinotta M, Manrique S, Guazzotti A, et al. Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development. <i>Development</i>. 2016;143(23):4419-4424. doi:<a href=\"https://doi.org/10.1242/dev.143545\">10.1242/dev.143545</a>","mla":"Cucinotta, Mara, et al. “Cytokinin Response Factors Integrate Auxin and Cytokinin Pathways for Female Reproductive Organ Development.” <i>Development</i>, vol. 143, no. 23, Company of Biologists, 2016, pp. 4419–24, doi:<a href=\"https://doi.org/10.1242/dev.143545\">10.1242/dev.143545</a>.","apa":"Cucinotta, M., Manrique, S., Guazzotti, A., Quadrelli, N., Mendes, M., Benková, E., &#38; Colombo, L. (2016). Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.143545\">https://doi.org/10.1242/dev.143545</a>","short":"M. Cucinotta, S. Manrique, A. Guazzotti, N. Quadrelli, M. Mendes, E. Benková, L. Colombo, Development 143 (2016) 4419–4424.","ista":"Cucinotta M, Manrique S, Guazzotti A, Quadrelli N, Mendes M, Benková E, Colombo L. 2016. Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development. Development. 143(23), 4419–4424."},"year":"2016","quality_controlled":"1","date_created":"2018-12-11T11:50:36Z","publist_id":"6168","abstract":[{"lang":"eng","text":"The developmental programme of the pistil is under the control of both auxin and cytokinin. Crosstalk between these factors converges on regulation of the auxin carrier PIN-FORMED 1 (PIN1). Here, we show that in the triple transcription factor mutant cytokinin response factor 2 (crf2) crf3 crf6 both pistil length and ovule number were reduced. PIN1 expression was also lower in the triple mutant and the phenotypes could not be rescued by exogenous cytokinin application. pin1 complementation studies using genomic PIN1 constructs showed that the pistil phenotypes were only rescued when the PCRE1 domain, to which CRFs bind, was present. Without this domain, pin mutants resemble the crf2 crf3 crf6 triple mutant, indicating the pivotal role of CRFs in auxin-cytokinin crosstalk."}],"month":"12","volume":143,"oa_version":"None","title":"Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development","date_updated":"2025-09-22T09:46:43Z","author":[{"full_name":"Cucinotta, Mara","last_name":"Cucinotta","first_name":"Mara"},{"full_name":"Manrique, Silvia","last_name":"Manrique","first_name":"Silvia"},{"first_name":"Andrea","last_name":"Guazzotti","full_name":"Guazzotti, Andrea"},{"last_name":"Quadrelli","full_name":"Quadrelli, Nadia","first_name":"Nadia"},{"first_name":"Marta","full_name":"Mendes, Marta","last_name":"Mendes"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Lucia","full_name":"Colombo, Lucia","last_name":"Colombo"}],"day":"01","scopus_import":"1","doi":"10.1242/dev.143545","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}],"external_id":{"isi":["000393454100012"]},"status":"public","article_processing_charge":"No","acknowledgement":"M.C. was funded by a PhD fellowship from the Università degli Studi di Milano-Bicocca and from Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR) [MIUR-PRIN 2012]. L.C. is also supported by MIUR [MIUR-PRIN 2012]. We would like to thank Andrew MacCabe and Edward Kiegle for editing the paper.","issue":"23","publisher":"Company of Biologists","_id":"1185","date_published":"2016-12-01T00:00:00Z"},{"language":[{"iso":"eng"}],"department":[{"_id":"EvBe"}],"doi":"10.1007/978-1-4939-6469-7_1","scopus_import":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","day":"19","author":[{"first_name":"Qiang","id":"40A4B9E6-F248-11E8-B48F-1D18A9856A87","last_name":"Zhu","full_name":"Zhu, Qiang"},{"full_name":"Žádníková, Petra","last_name":"Žádníková","first_name":"Petra"},{"first_name":"Dajo","full_name":"Smet, Dajo","last_name":"Smet"},{"first_name":"Dominique","full_name":"Van Der Straeten, Dominique","last_name":"Van Der Straeten"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"date_updated":"2021-01-12T06:49:07Z","alternative_title":["Methods in Molecular Biology"],"title":"Real time analysis of the apical hook development","intvolume":"      1497","publication_status":"published","oa_version":"None","volume":1497,"date_published":"2016-11-19T00:00:00Z","_id":"1210","month":"11","abstract":[{"text":"Mechanisms for cell protection are essential for survival of multicellular organisms. In plants, the apical hook, which is transiently formed in darkness when the germinating seedling penetrates towards the soil surface, plays such protective role and shields the vitally important shoot apical meristem and cotyledons from damage. The apical hook is formed by bending of the upper hypocotyl soon after germination, and it is maintained in a closed stage while the hypocotyl continues to penetrate through the soil and rapidly opens when exposed to light in proximity of the soil surface. To uncover the complex molecular network orchestrating this spatiotemporally tightly coordinated process, monitoring of the apical hook development in real time is indispensable. Here we describe an imaging platform that enables high-resolution kinetic analysis of this dynamic developmental process. © Springer Science+Business Media New York 2017.","lang":"eng"}],"publisher":"Humana Press","publist_id":"6135","date_created":"2018-12-11T11:50:44Z","quality_controlled":"1","citation":{"ieee":"Q. Zhu, P. Žádníková, D. Smet, D. Van Der Straeten, and E. Benková, “Real time analysis of the apical hook development,” in <i>Plant Hormones</i>, vol. 1497, Humana Press, 2016, pp. 1–8.","chicago":"Zhu, Qiang, Petra Žádníková, Dajo Smet, Dominique Van Der Straeten, and Eva Benková. “Real Time Analysis of the Apical Hook Development.” In <i>Plant Hormones</i>, 1497:1–8. Humana Press, 2016. <a href=\"https://doi.org/10.1007/978-1-4939-6469-7_1\">https://doi.org/10.1007/978-1-4939-6469-7_1</a>.","mla":"Zhu, Qiang, et al. “Real Time Analysis of the Apical Hook Development.” <i>Plant Hormones</i>, vol. 1497, Humana Press, 2016, pp. 1–8, doi:<a href=\"https://doi.org/10.1007/978-1-4939-6469-7_1\">10.1007/978-1-4939-6469-7_1</a>.","short":"Q. Zhu, P. Žádníková, D. Smet, D. Van Der Straeten, E. Benková, in:, Plant Hormones, Humana Press, 2016, pp. 1–8.","apa":"Zhu, Q., Žádníková, P., Smet, D., Van Der Straeten, D., &#38; Benková, E. (2016). Real time analysis of the apical hook development. In <i>Plant Hormones</i> (Vol. 1497, pp. 1–8). Humana Press. <a href=\"https://doi.org/10.1007/978-1-4939-6469-7_1\">https://doi.org/10.1007/978-1-4939-6469-7_1</a>","ama":"Zhu Q, Žádníková P, Smet D, Van Der Straeten D, Benková E. Real time analysis of the apical hook development. In: <i>Plant Hormones</i>. Vol 1497. Humana Press; 2016:1-8. doi:<a href=\"https://doi.org/10.1007/978-1-4939-6469-7_1\">10.1007/978-1-4939-6469-7_1</a>","ista":"Zhu Q, Žádníková P, Smet D, Van Der Straeten D, Benková E. 2016.Real time analysis of the apical hook development. In: Plant Hormones. Methods in Molecular Biology, vol. 1497, 1–8."},"year":"2016","acknowledgement":"We thank Herman  \r\nHöfte \r\n, Todor Asenov, Robert Hauschield, and \r\nMarcal  Gallemi  for  help  with  the  establishment  of  the  real-time  \r\nimaging platform and technical support. This work was supported \r\nby the Czech Science Foundation (GA13-39982S) to Eva Benková. \r\nDominique   Van   Der   Straeten   acknowledges   the   Research   \r\nFoundation  Flanders  for  fi\r\n  nancial  support  (G.0656.13N).  Dajo  \r\nSmet holds a PhD fellowship of the Research Foundation Flanders. ","page":"1 - 8","publication":"Plant Hormones","status":"public","type":"book_chapter"}]