---
_id: '15179'
abstract:
- lang: eng
text: The fungal bioluminescence pathway can be reconstituted in other organisms
allowing luminescence imaging without exogenously supplied substrate. The pathway
starts from hispidin biosynthesis—a step catalyzed by a large fungal polyketide
synthase that requires a posttranslational modification for activity. Here, we
report identification of alternative compact hispidin synthases encoded by a phylogenetically
diverse group of plants. A hybrid bioluminescence pathway that combines plant
and fungal genes is more compact, not dependent on availability of machinery for
posttranslational modifications, and confers autonomous bioluminescence in yeast,
mammalian, and plant hosts. The compact size of plant hispidin synthases enables
additional modes of delivery of autoluminescence, such as delivery with viral
vectors.
acknowledgement: "We thank Milaboratory (milaboratory.com) for the access to computing
and storage infrastructure. We thank J. Petrasek for providing the BY-2 cell culture
line. We thank Konstantin Lukyanov laboratory and Sergey Deyev laboratory for assistance
with experiments.\r\nThis study was partially funded by Light Bio and Planta. The
Synthetic biology Group is funded by the MRC London Institute of Medical Sciences
(UKRI MC-A658-5QEA0). Cloning and luminescent assays performed in BY-2 were partially
supported by RSF, project number 22-14-00400, https://rscf.ru/project/22-14-00400/.
Plant transformations were funded by RFBR and MOST, project number 21-54-52004.
Plant imaging experiments were funded by RSF, project number 22-74-00124, https://rscf.ru/project/22-74-00124/.
Viral delivery experiments were funded by the grant PID2019-108203RB-I00 Plan Nacional
I + D from the Ministerio de Ciencia e Innovación (Spain) through the Agencia Estatal
de Investigación (cofinanced by the European Regional Development Fund)."
article_number: adk1992
article_processing_charge: Yes
article_type: original
author:
- first_name: Kseniia A.
full_name: Palkina, Kseniia A.
last_name: Palkina
- first_name: Tatiana A.
full_name: Karataeva, Tatiana A.
last_name: Karataeva
- first_name: Maxim M.
full_name: Perfilov, Maxim M.
last_name: Perfilov
- first_name: Liliia I.
full_name: Fakhranurova, Liliia I.
last_name: Fakhranurova
- first_name: Nadezhda M.
full_name: Markina, Nadezhda M.
last_name: Markina
- first_name: Louisa
full_name: Gonzalez Somermeyer, Louisa
id: 4720D23C-F248-11E8-B48F-1D18A9856A87
last_name: Gonzalez Somermeyer
orcid: 0000-0001-9139-5383
- first_name: Elena
full_name: Garcia-Perez, Elena
last_name: Garcia-Perez
- first_name: Marta
full_name: Vazquez-Vilar, Marta
last_name: Vazquez-Vilar
- first_name: Marta
full_name: Rodriguez-Rodriguez, Marta
last_name: Rodriguez-Rodriguez
- first_name: Victor
full_name: Vazquez-Vilriales, Victor
last_name: Vazquez-Vilriales
- first_name: Ekaterina S.
full_name: Shakhova, Ekaterina S.
last_name: Shakhova
- first_name: Tatiana
full_name: Mitiouchkina, Tatiana
last_name: Mitiouchkina
- first_name: Olga A.
full_name: Belozerova, Olga A.
last_name: Belozerova
- first_name: Sergey I.
full_name: Kovalchuk, Sergey I.
last_name: Kovalchuk
- first_name: Anna
full_name: Alekberova, Anna
last_name: Alekberova
- first_name: Alena K.
full_name: Malyshevskaia, Alena K.
last_name: Malyshevskaia
- first_name: Evgenia N.
full_name: Bugaeva, Evgenia N.
last_name: Bugaeva
- first_name: Elena B.
full_name: Guglya, Elena B.
last_name: Guglya
- first_name: Anastasia
full_name: Balakireva, Anastasia
last_name: Balakireva
- first_name: Nikita
full_name: Sytov, Nikita
last_name: Sytov
- first_name: Anastasia
full_name: Bezlikhotnova, Anastasia
last_name: Bezlikhotnova
- first_name: Daria I.
full_name: Boldyreva, Daria I.
last_name: Boldyreva
- first_name: Vladislav V.
full_name: Babenko, Vladislav V.
last_name: Babenko
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Vladimir V.
full_name: Choob, Vladimir V.
last_name: Choob
- first_name: Diego
full_name: Orzaez, Diego
last_name: Orzaez
- first_name: Ilia V.
full_name: Yampolsky, Ilia V.
last_name: Yampolsky
- first_name: Alexander S.
full_name: Mishin, Alexander S.
last_name: Mishin
- first_name: Karen S.
full_name: Sarkisyan, Karen S.
last_name: Sarkisyan
citation:
ama: Palkina KA, Karataeva TA, Perfilov MM, et al. A hybrid pathway for self-sustained
luminescence. Science Advances. 2024;10(10). doi:10.1126/sciadv.adk1992
apa: Palkina, K. A., Karataeva, T. A., Perfilov, M. M., Fakhranurova, L. I., Markina,
N. M., Gonzalez Somermeyer, L., … Sarkisyan, K. S. (2024). A hybrid pathway for
self-sustained luminescence. Science Advances. American Association for
the Advancement of Science. https://doi.org/10.1126/sciadv.adk1992
chicago: Palkina, Kseniia A., Tatiana A. Karataeva, Maxim M. Perfilov, Liliia I.
Fakhranurova, Nadezhda M. Markina, Louisa Gonzalez Somermeyer, Elena Garcia-Perez,
et al. “A Hybrid Pathway for Self-Sustained Luminescence.” Science Advances.
American Association for the Advancement of Science, 2024. https://doi.org/10.1126/sciadv.adk1992.
ieee: K. A. Palkina et al., “A hybrid pathway for self-sustained luminescence,”
Science Advances, vol. 10, no. 10. American Association for the Advancement
of Science, 2024.
ista: Palkina KA, Karataeva TA, Perfilov MM, Fakhranurova LI, Markina NM, Gonzalez
Somermeyer L, Garcia-Perez E, Vazquez-Vilar M, Rodriguez-Rodriguez M, Vazquez-Vilriales
V, Shakhova ES, Mitiouchkina T, Belozerova OA, Kovalchuk SI, Alekberova A, Malyshevskaia
AK, Bugaeva EN, Guglya EB, Balakireva A, Sytov N, Bezlikhotnova A, Boldyreva DI,
Babenko VV, Kondrashov F, Choob VV, Orzaez D, Yampolsky IV, Mishin AS, Sarkisyan
KS. 2024. A hybrid pathway for self-sustained luminescence. Science Advances.
10(10), adk1992.
mla: Palkina, Kseniia A., et al. “A Hybrid Pathway for Self-Sustained Luminescence.”
Science Advances, vol. 10, no. 10, adk1992, American Association for the
Advancement of Science, 2024, doi:10.1126/sciadv.adk1992.
short: K.A. Palkina, T.A. Karataeva, M.M. Perfilov, L.I. Fakhranurova, N.M. Markina,
L. Gonzalez Somermeyer, E. Garcia-Perez, M. Vazquez-Vilar, M. Rodriguez-Rodriguez,
V. Vazquez-Vilriales, E.S. Shakhova, T. Mitiouchkina, O.A. Belozerova, S.I. Kovalchuk,
A. Alekberova, A.K. Malyshevskaia, E.N. Bugaeva, E.B. Guglya, A. Balakireva, N.
Sytov, A. Bezlikhotnova, D.I. Boldyreva, V.V. Babenko, F. Kondrashov, V.V. Choob,
D. Orzaez, I.V. Yampolsky, A.S. Mishin, K.S. Sarkisyan, Science Advances 10 (2024).
date_created: 2024-03-25T08:54:33Z
date_published: 2024-03-01T00:00:00Z
date_updated: 2024-03-25T09:44:53Z
day: '01'
ddc:
- '580'
department:
- _id: FyKo
doi: 10.1126/sciadv.adk1992
file:
- access_level: open_access
checksum: a19c43b260ea0bbaf895a29712e3153c
content_type: application/pdf
creator: dernst
date_created: 2024-03-25T09:42:10Z
date_updated: 2024-03-25T09:42:10Z
file_id: '15185'
file_name: 2024_ScienceAdv_Palkina.pdf
file_size: 1499302
relation: main_file
success: 1
file_date_updated: 2024-03-25T09:42:10Z
has_accepted_license: '1'
intvolume: ' 10'
issue: '10'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
publication: Science Advances
publication_identifier:
issn:
- 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: A hybrid pathway for self-sustained luminescence
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 10
year: '2024'
...
---
_id: '12758'
abstract:
- lang: eng
text: AlphaFold changed the field of structural biology by achieving three-dimensional
(3D) structure prediction from protein sequence at experimental quality. The astounding
success even led to claims that the protein folding problem is “solved”. However,
protein folding problem is more than just structure prediction from sequence.
Presently, it is unknown if the AlphaFold-triggered revolution could help to solve
other problems related to protein folding. Here we assay the ability of AlphaFold
to predict the impact of single mutations on protein stability (ΔΔG) and function.
To study the question we extracted the pLDDT and metrics from AlphaFold
predictions before and after single mutation in a protein and correlated the predicted
change with the experimentally known ΔΔG values. Additionally, we correlated the
same AlphaFold pLDDT metrics with the impact of a single mutation on structure
using a large scale dataset of single mutations in GFP with the experimentally
assayed levels of fluorescence. We found a very weak or no correlation between
AlphaFold output metrics and change of protein stability or fluorescence. Our
results imply that AlphaFold may not be immediately applied to other problems
or applications in protein folding.
acknowledgement: The authors acknowledge the use of Zhores supercomputer [28] for
obtaining the results presented in this paper.The authors thank Zimin Foundation
and Petrovax for support of the presented study at the School of Molecular and Theoretical
Biology 2021.
article_number: e0282689
article_processing_charge: No
article_type: original
author:
- first_name: Marina A.
full_name: Pak, Marina A.
last_name: Pak
- first_name: Karina A.
full_name: Markhieva, Karina A.
last_name: Markhieva
- first_name: Mariia S.
full_name: Novikova, Mariia S.
last_name: Novikova
- first_name: Dmitry S.
full_name: Petrov, Dmitry S.
last_name: Petrov
- first_name: Ilya S.
full_name: Vorobyev, Ilya S.
last_name: Vorobyev
- first_name: Ekaterina
full_name: Maksimova, Ekaterina
id: 2FBE0DE4-F248-11E8-B48F-1D18A9856A87
last_name: Maksimova
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Dmitry N.
full_name: Ivankov, Dmitry N.
last_name: Ivankov
citation:
ama: Pak MA, Markhieva KA, Novikova MS, et al. Using AlphaFold to predict the impact
of single mutations on protein stability and function. PLoS ONE. 2023;18(3).
doi:10.1371/journal.pone.0282689
apa: Pak, M. A., Markhieva, K. A., Novikova, M. S., Petrov, D. S., Vorobyev, I.
S., Maksimova, E., … Ivankov, D. N. (2023). Using AlphaFold to predict the impact
of single mutations on protein stability and function. PLoS ONE. Public
Library of Science. https://doi.org/10.1371/journal.pone.0282689
chicago: Pak, Marina A., Karina A. Markhieva, Mariia S. Novikova, Dmitry S. Petrov,
Ilya S. Vorobyev, Ekaterina Maksimova, Fyodor Kondrashov, and Dmitry N. Ivankov.
“Using AlphaFold to Predict the Impact of Single Mutations on Protein Stability
and Function.” PLoS ONE. Public Library of Science, 2023. https://doi.org/10.1371/journal.pone.0282689.
ieee: M. A. Pak et al., “Using AlphaFold to predict the impact of single
mutations on protein stability and function,” PLoS ONE, vol. 18, no. 3.
Public Library of Science, 2023.
ista: Pak MA, Markhieva KA, Novikova MS, Petrov DS, Vorobyev IS, Maksimova E, Kondrashov
F, Ivankov DN. 2023. Using AlphaFold to predict the impact of single mutations
on protein stability and function. PLoS ONE. 18(3), e0282689.
mla: Pak, Marina A., et al. “Using AlphaFold to Predict the Impact of Single Mutations
on Protein Stability and Function.” PLoS ONE, vol. 18, no. 3, e0282689,
Public Library of Science, 2023, doi:10.1371/journal.pone.0282689.
short: M.A. Pak, K.A. Markhieva, M.S. Novikova, D.S. Petrov, I.S. Vorobyev, E. Maksimova,
F. Kondrashov, D.N. Ivankov, PLoS ONE 18 (2023).
date_created: 2023-03-26T22:01:07Z
date_published: 2023-03-16T00:00:00Z
date_updated: 2023-08-01T13:47:14Z
day: '16'
ddc:
- '570'
department:
- _id: FyKo
- _id: MaRo
doi: 10.1371/journal.pone.0282689
external_id:
isi:
- '000985134400106'
file:
- access_level: open_access
checksum: 0281bdfccf8d76c4e08dd011c603f6b6
content_type: application/pdf
creator: dernst
date_created: 2023-03-27T07:09:08Z
date_updated: 2023-03-27T07:09:08Z
file_id: '12771'
file_name: 2023_PLoSOne_Pak.pdf
file_size: 856625
relation: main_file
success: 1
file_date_updated: 2023-03-27T07:09:08Z
has_accepted_license: '1'
intvolume: ' 18'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
publication: PLoS ONE
publication_identifier:
eissn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Using AlphaFold to predict the impact of single mutations on protein stability
and function
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 18
year: '2023'
...
---
_id: '13164'
abstract:
- lang: eng
text: Molecular compatibility between gametes is a prerequisite for successful fertilization.
As long as a sperm and egg can recognize and bind each other via their surface
proteins, gamete fusion may occur even between members of separate species, resulting
in hybrids that can impact speciation. The egg membrane protein Bouncer confers
species specificity to gamete interactions between medaka and zebrafish, preventing
their cross-fertilization. Here, we leverage this specificity to uncover distinct
amino acid residues and N-glycosylation patterns that differentially influence
the function of medaka and zebrafish Bouncer and contribute to cross-species incompatibility.
Curiously, in contrast to the specificity observed for medaka and zebrafish Bouncer,
seahorse and fugu Bouncer are compatible with both zebrafish and medaka sperm,
in line with the pervasive purifying selection that dominates Bouncer’s evolution.
The Bouncer-sperm interaction is therefore the product of seemingly opposing evolutionary
forces that, for some species, restrict fertilization to closely related fish,
and for others, allow broad gamete compatibility that enables hybridization.
acknowledgement: We thank Manfred Schartl for sharing RNA-seq data from medaka ovaries
and testes prior to publication; Maria Novatchkova for help with RNA-seq analysis;
Katharina Lust for advice on medaka techniques; Milan Malinsky for input on Lake
Malawi cichlid Bouncer sequences; Felicia Spitzer, Mirjam Binner, and Anna Bandura
for help with genotyping; Friedrich Puhl, Kerstin Rattner, Julia Koenig, and Dijana
Sunjic for taking care of zebrafish and medaka; and the Pauli lab for helpful discussions
about the project and feedback on the manuscript. K.R.B.G. was supported by a DOC
Fellowship from the Austrian Academy of Sciences. Work in the Pauli lab was supported
by the FWF START program (Y 1031-B28 to A.P.), the ERC CoG 101044495/GaMe, the HFSP
Career Development Award (CDA00066/2015 to A.P.), a HFSP Young Investigator Award
(RGY0079/2020 to A.P.) and the FWF SFB RNA-Deco (project number F80). The IMP receives
institutional funding from Boehringer Ingelheim and the Austrian Research Promotion
Agency (Headquarter grant FFG-852936). Work by J.S. and Y.M. in this project was
supported by the Israel Science Foundation grant 636/21 to Y.M. Work by L.J. was
supported by the Swedish Research Council grant 2020-04936 and the Knut and Alice
Wallenberg Foundation grant 2018.0042. For the purpose of Open Access, the author
has applied a CC BY public copyright license to any Author Accepted Manuscript (AAM)
version arising from this submission.
article_number: '3506'
article_processing_charge: No
article_type: original
author:
- first_name: Krista R.B.
full_name: Gert, Krista R.B.
last_name: Gert
- first_name: Karin
full_name: Panser, Karin
last_name: Panser
- first_name: Joachim
full_name: Surm, Joachim
last_name: Surm
- first_name: Benjamin S.
full_name: Steinmetz, Benjamin S.
last_name: Steinmetz
- first_name: Alexander
full_name: Schleiffer, Alexander
last_name: Schleiffer
- first_name: Luca
full_name: Jovine, Luca
last_name: Jovine
- first_name: Yehu
full_name: Moran, Yehu
last_name: Moran
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Andrea
full_name: Pauli, Andrea
last_name: Pauli
citation:
ama: Gert KRB, Panser K, Surm J, et al. Divergent molecular signatures in fish Bouncer
proteins define cross-fertilization boundaries. Nature Communications.
2023;14. doi:10.1038/s41467-023-39317-4
apa: Gert, K. R. B., Panser, K., Surm, J., Steinmetz, B. S., Schleiffer, A., Jovine,
L., … Pauli, A. (2023). Divergent molecular signatures in fish Bouncer proteins
define cross-fertilization boundaries. Nature Communications. Springer
Nature. https://doi.org/10.1038/s41467-023-39317-4
chicago: Gert, Krista R.B., Karin Panser, Joachim Surm, Benjamin S. Steinmetz, Alexander
Schleiffer, Luca Jovine, Yehu Moran, Fyodor Kondrashov, and Andrea Pauli. “Divergent
Molecular Signatures in Fish Bouncer Proteins Define Cross-Fertilization Boundaries.”
Nature Communications. Springer Nature, 2023. https://doi.org/10.1038/s41467-023-39317-4.
ieee: K. R. B. Gert et al., “Divergent molecular signatures in fish Bouncer
proteins define cross-fertilization boundaries,” Nature Communications,
vol. 14. Springer Nature, 2023.
ista: Gert KRB, Panser K, Surm J, Steinmetz BS, Schleiffer A, Jovine L, Moran Y,
Kondrashov F, Pauli A. 2023. Divergent molecular signatures in fish Bouncer proteins
define cross-fertilization boundaries. Nature Communications. 14, 3506.
mla: Gert, Krista R. B., et al. “Divergent Molecular Signatures in Fish Bouncer
Proteins Define Cross-Fertilization Boundaries.” Nature Communications,
vol. 14, 3506, Springer Nature, 2023, doi:10.1038/s41467-023-39317-4.
short: K.R.B. Gert, K. Panser, J. Surm, B.S. Steinmetz, A. Schleiffer, L. Jovine,
Y. Moran, F. Kondrashov, A. Pauli, Nature Communications 14 (2023).
date_created: 2023-06-25T22:00:45Z
date_published: 2023-06-14T00:00:00Z
date_updated: 2023-12-13T11:26:34Z
day: '14'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1038/s41467-023-39317-4
external_id:
isi:
- '001048208600023'
file:
- access_level: open_access
checksum: d6165f41c7f1c2c04b04256ec9f003fb
content_type: application/pdf
creator: dernst
date_created: 2023-06-26T10:26:04Z
date_updated: 2023-06-26T10:26:04Z
file_id: '13172'
file_name: 2023_NatureComm_Gert.pdf
file_size: 1555006
relation: main_file
success: 1
file_date_updated: 2023-06-26T10:26:04Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Divergent molecular signatures in fish Bouncer proteins define cross-fertilization
boundaries
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2023'
...
---
_id: '13976'
abstract:
- lang: eng
text: Conflicts and natural disasters affect entire populations of the countries
involved and, in addition to the thousands of lives destroyed, have a substantial
negative impact on the scientific advances these countries provide. The unprovoked
invasion of Ukraine by Russia, the devastating earthquake in Turkey and Syria,
and the ongoing conflicts in the Middle East are just a few examples. Millions
of people have been killed or displaced, their futures uncertain. These events
have resulted in extensive infrastructure collapse, with loss of electricity,
transportation, and access to services. Schools, universities, and research centers
have been destroyed along with decades’ worth of data, samples, and findings.
Scholars in disaster areas face short- and long-term problems in terms of what
they can accomplish now for obtaining grants and for employment in the long run.
In our interconnected world, conflicts and disasters are no longer a local problem
but have wide-ranging impacts on the entire world, both now and in the future.
Here, we focus on the current and ongoing impact of war on the scientific community
within Ukraine and from this draw lessons that can be applied to all affected
countries where scientists at risk are facing hardship. We present and classify
examples of effective and feasible mechanisms used to support researchers in countries
facing hardship and discuss how these can be implemented with help from the international
scientific community and what more is desperately needed. Reaching out, providing
accessible training opportunities, and developing collaborations should increase
inclusion and connectivity, support scientific advancements within affected communities,
and expedite postwar and disaster recovery.
acknowledgement: "Our article is dedicated to all freedom-loving people around the
world and to the people of Ukraine who fight for our freedom. Special thanks to
Anita Bandrowski, Oleksandra V. Ivashchenko, and Sanita Reinsone for the helpful
review, valuable criticism, and useful suggestions while preparing this manuscript,
and to Tetiana Yes'kova for helping with Ukrainian translation.\r\nAll authors volunteered
their time. No funding supported work on this article."
article_processing_charge: Yes
article_type: original
author:
- first_name: Walter
full_name: Wolfsberger, Walter
last_name: Wolfsberger
- first_name: Karishma
full_name: Chhugani, Karishma
last_name: Chhugani
- first_name: Khrystyna
full_name: Shchubelka, Khrystyna
last_name: Shchubelka
- first_name: Alina
full_name: Frolova, Alina
last_name: Frolova
- first_name: Yuriy
full_name: Salyha, Yuriy
last_name: Salyha
- first_name: Oksana
full_name: Zlenko, Oksana
last_name: Zlenko
- first_name: Mykhailo
full_name: Arych, Mykhailo
last_name: Arych
- first_name: Dmytro
full_name: Dziuba, Dmytro
last_name: Dziuba
- first_name: Andrii
full_name: Parkhomenko, Andrii
last_name: Parkhomenko
- first_name: Volodymyr
full_name: Smolanka, Volodymyr
last_name: Smolanka
- first_name: Zeynep H.
full_name: Gümüş, Zeynep H.
last_name: Gümüş
- first_name: Efe
full_name: Sezgin, Efe
last_name: Sezgin
- first_name: Alondra
full_name: Diaz-Lameiro, Alondra
last_name: Diaz-Lameiro
- first_name: Viktor R.
full_name: Toth, Viktor R.
last_name: Toth
- first_name: Megi
full_name: Maci, Megi
last_name: Maci
- first_name: Eric
full_name: Bortz, Eric
last_name: Bortz
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Patricia M.
full_name: Morton, Patricia M.
last_name: Morton
- first_name: Paweł P.
full_name: Łabaj, Paweł P.
last_name: Łabaj
- first_name: Veronika
full_name: Romero, Veronika
last_name: Romero
- first_name: Jakub
full_name: Hlávka, Jakub
last_name: Hlávka
- first_name: Serghei
full_name: Mangul, Serghei
last_name: Mangul
- first_name: Taras K.
full_name: Oleksyk, Taras K.
last_name: Oleksyk
citation:
ama: 'Wolfsberger W, Chhugani K, Shchubelka K, et al. Scientists without borders:
Lessons from Ukraine. GigaScience. 2023;12. doi:10.1093/gigascience/giad045'
apa: 'Wolfsberger, W., Chhugani, K., Shchubelka, K., Frolova, A., Salyha, Y., Zlenko,
O., … Oleksyk, T. K. (2023). Scientists without borders: Lessons from Ukraine.
GigaScience. Oxford Academic. https://doi.org/10.1093/gigascience/giad045'
chicago: 'Wolfsberger, Walter, Karishma Chhugani, Khrystyna Shchubelka, Alina Frolova,
Yuriy Salyha, Oksana Zlenko, Mykhailo Arych, et al. “Scientists without Borders:
Lessons from Ukraine.” GigaScience. Oxford Academic, 2023. https://doi.org/10.1093/gigascience/giad045.'
ieee: 'W. Wolfsberger et al., “Scientists without borders: Lessons from Ukraine,”
GigaScience, vol. 12. Oxford Academic, 2023.'
ista: 'Wolfsberger W, Chhugani K, Shchubelka K, Frolova A, Salyha Y, Zlenko O, Arych
M, Dziuba D, Parkhomenko A, Smolanka V, Gümüş ZH, Sezgin E, Diaz-Lameiro A, Toth
VR, Maci M, Bortz E, Kondrashov F, Morton PM, Łabaj PP, Romero V, Hlávka J, Mangul
S, Oleksyk TK. 2023. Scientists without borders: Lessons from Ukraine. GigaScience.
12.'
mla: 'Wolfsberger, Walter, et al. “Scientists without Borders: Lessons from Ukraine.”
GigaScience, vol. 12, Oxford Academic, 2023, doi:10.1093/gigascience/giad045.'
short: W. Wolfsberger, K. Chhugani, K. Shchubelka, A. Frolova, Y. Salyha, O. Zlenko,
M. Arych, D. Dziuba, A. Parkhomenko, V. Smolanka, Z.H. Gümüş, E. Sezgin, A. Diaz-Lameiro,
V.R. Toth, M. Maci, E. Bortz, F. Kondrashov, P.M. Morton, P.P. Łabaj, V. Romero,
J. Hlávka, S. Mangul, T.K. Oleksyk, GigaScience 12 (2023).
date_created: 2023-08-06T22:01:13Z
date_published: 2023-07-27T00:00:00Z
date_updated: 2023-12-13T12:01:46Z
day: '27'
department:
- _id: FyKo
doi: 10.1093/gigascience/giad045
external_id:
isi:
- '001081086100001'
pmid:
- '37496156'
intvolume: ' 12'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1093/gigascience/giad045
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
publication: GigaScience
publication_identifier:
eissn:
- 2047-217X
publication_status: epub_ahead
publisher: Oxford Academic
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Scientists without borders: Lessons from Ukraine'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2023'
...
---
_id: '14716'
abstract:
- lang: eng
text: "Background: Antimicrobial resistance (AMR) poses a significant global health
threat, and an accurate prediction of bacterial resistance patterns is critical
for effective treatment and control strategies. In recent years, machine learning
(ML) approaches have emerged as powerful tools for analyzing large-scale bacterial
AMR data. However, ML methods often ignore evolutionary relationships among bacterial
strains, which can greatly impact performance of the ML methods, especially if
resistance-associated features are attempted to be detected. Genome-wide association
studies (GWAS) methods like linear mixed models accounts for the evolutionary
relationships in bacteria, but they uncover only highly significant variants which
have already been reported in literature.\r\n\r\nResults: In this work, we introduce
a novel phylogeny-related parallelism score (PRPS), which measures whether a certain
feature is correlated with the population structure of a set of samples. We demonstrate
that PRPS can be used, in combination with SVM- and random forest-based models,
to reduce the number of features in the analysis, while simultaneously increasing
models’ performance. We applied our pipeline to publicly available AMR data from
PATRIC database for Mycobacterium tuberculosis against six common antibiotics.\r\n\r\nConclusions:
Using our pipeline, we re-discovered known resistance-associated mutations as
well as new candidate mutations which can be related to resistance and not previously
reported in the literature. We demonstrated that taking into account phylogenetic
relationships not only improves the model performance, but also yields more biologically
relevant predicted most contributing resistance markers."
acknowledgement: Open Access funding enabled and organized by Projekt DEAL. A.Y. and
O.V.K. acknowledge financial support from the Klaus Faber Foundation. A.A.A. was
funded by the Helmholtz AI project AMR-XAI. The work of O.O.B. is funded by Fonds
zur Förderung der Wissenschaftlichen Forschung (FWF), Grant ESP 253-B.
article_number: '404'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alper
full_name: Yurtseven, Alper
last_name: Yurtseven
- first_name: Sofia
full_name: Buyanova, Sofia
id: 2F54A7BC-3902-11EA-AC87-BC9F3DDC885E
last_name: Buyanova
- first_name: Amay Ajaykumar A.
full_name: Agrawal, Amay Ajaykumar A.
last_name: Agrawal
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Olga V V.
full_name: Kalinina, Olga V V.
last_name: Kalinina
citation:
ama: Yurtseven A, Buyanova S, Agrawal AAA, Bochkareva O, Kalinina OVV. Machine learning
and phylogenetic analysis allow for predicting antibiotic resistance in M. tuberculosis.
BMC Microbiology. 2023;23(1). doi:10.1186/s12866-023-03147-7
apa: Yurtseven, A., Buyanova, S., Agrawal, A. A. A., Bochkareva, O., & Kalinina,
O. V. V. (2023). Machine learning and phylogenetic analysis allow for predicting
antibiotic resistance in M. tuberculosis. BMC Microbiology. Springer Nature.
https://doi.org/10.1186/s12866-023-03147-7
chicago: Yurtseven, Alper, Sofia Buyanova, Amay Ajaykumar A. Agrawal, Olga Bochkareva,
and Olga V V. Kalinina. “Machine Learning and Phylogenetic Analysis Allow for
Predicting Antibiotic Resistance in M. Tuberculosis.” BMC Microbiology.
Springer Nature, 2023. https://doi.org/10.1186/s12866-023-03147-7.
ieee: A. Yurtseven, S. Buyanova, A. A. A. Agrawal, O. Bochkareva, and O. V. V. Kalinina,
“Machine learning and phylogenetic analysis allow for predicting antibiotic resistance
in M. tuberculosis,” BMC Microbiology, vol. 23, no. 1. Springer Nature,
2023.
ista: Yurtseven A, Buyanova S, Agrawal AAA, Bochkareva O, Kalinina OVV. 2023. Machine
learning and phylogenetic analysis allow for predicting antibiotic resistance
in M. tuberculosis. BMC Microbiology. 23(1), 404.
mla: Yurtseven, Alper, et al. “Machine Learning and Phylogenetic Analysis Allow
for Predicting Antibiotic Resistance in M. Tuberculosis.” BMC Microbiology,
vol. 23, no. 1, 404, Springer Nature, 2023, doi:10.1186/s12866-023-03147-7.
short: A. Yurtseven, S. Buyanova, A.A.A. Agrawal, O. Bochkareva, O.V.V. Kalinina,
BMC Microbiology 23 (2023).
date_created: 2023-12-31T23:01:02Z
date_published: 2023-12-01T00:00:00Z
date_updated: 2024-01-02T09:20:57Z
day: '01'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1186/s12866-023-03147-7
external_id:
pmid:
- '38124060'
file:
- access_level: open_access
checksum: 7ff5e95f3496ff663301eb4a13a316d5
content_type: application/pdf
creator: dernst
date_created: 2024-01-02T09:09:32Z
date_updated: 2024-01-02T09:09:32Z
file_id: '14723'
file_name: 2023_BMCMicrobiology_Yurtseven.pdf
file_size: 1979922
relation: main_file
success: 1
file_date_updated: 2024-01-02T09:09:32Z
has_accepted_license: '1'
intvolume: ' 23'
issue: '1'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
publication: BMC Microbiology
publication_identifier:
eissn:
- 1471-2180
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Machine learning and phylogenetic analysis allow for predicting antibiotic
resistance in M. tuberculosis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2023'
...
---
_id: '10927'
abstract:
- lang: eng
text: "Motivation\r\nHigh plasticity of bacterial genomes is provided by numerous
mechanisms including horizontal gene transfer and recombination via numerous flanking
repeats. Genome rearrangements such as inversions, deletions, insertions and duplications
may independently occur in different strains, providing parallel adaptation or
phenotypic diversity. Specifically, such rearrangements might be responsible for
virulence, antibiotic resistance and antigenic variation. However, identification
of such events requires laborious manual inspection and verification of phyletic
pattern consistency.\r\nResults\r\nHere, we define the term ‘parallel rearrangements’
as events that occur independently in phylogenetically distant bacterial strains
and present a formalization of the problem of parallel rearrangements calling.
We implement an algorithmic solution for the identification of parallel rearrangements
in bacterial populations as a tool PaReBrick. The tool takes a collection of strains
represented as a sequence of oriented synteny blocks and a phylogenetic tree as
input data. It identifies rearrangements, tests them for consistency with a tree,
and sorts the events by their parallelism score. The tool provides diagrams of
the neighbors for each block of interest, allowing the detection of horizontally
transferred blocks or their extra copies and the inversions in which copied blocks
are involved. We demonstrated PaReBrick’s efficiency and accuracy and showed its
potential to detect genome rearrangements responsible for pathogenicity and adaptation
in bacterial genomes."
acknowledgement: "The authors thank the 2020 student class of the Bioinformatics Institute,
who\r\nused the first versions of the tool and provided many valuable suggestions
to\r\nimprove usability. They also thank Louisa Gonzalez Somermeyer for manuscript
proofreading\r\nThis work was supported by the National Center for Cognitive Research
of\r\nITMO University and JetBrains Research [to A.Z and N.A.]; and the European\r\nUnion’s
Horizon 2020 Research and Innovation Programme under the Marie\r\nSkłodowska-Curie
[754411 to O.B.].\r\nPaReBrick is written in Python and is available on GitHub:
https://github.com/ctlab/parallel-rearrangements."
article_processing_charge: No
article_type: original
author:
- first_name: Alexey
full_name: Zabelkin, Alexey
last_name: Zabelkin
- first_name: Yulia
full_name: Yakovleva, Yulia
last_name: Yakovleva
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Nikita
full_name: Alexeev, Nikita
last_name: Alexeev
citation:
ama: 'Zabelkin A, Yakovleva Y, Bochkareva O, Alexeev N. PaReBrick: PArallel REarrangements
and BReaks identification toolkit. Bioinformatics. 2022;38(2):357-363.
doi:10.1093/bioinformatics/btab691'
apa: 'Zabelkin, A., Yakovleva, Y., Bochkareva, O., & Alexeev, N. (2022). PaReBrick:
PArallel REarrangements and BReaks identification toolkit. Bioinformatics.
Oxford Academic. https://doi.org/10.1093/bioinformatics/btab691'
chicago: 'Zabelkin, Alexey, Yulia Yakovleva, Olga Bochkareva, and Nikita Alexeev.
“PaReBrick: PArallel REarrangements and BReaks Identification Toolkit.” Bioinformatics.
Oxford Academic, 2022. https://doi.org/10.1093/bioinformatics/btab691.'
ieee: 'A. Zabelkin, Y. Yakovleva, O. Bochkareva, and N. Alexeev, “PaReBrick: PArallel
REarrangements and BReaks identification toolkit,” Bioinformatics, vol.
38, no. 2. Oxford Academic, pp. 357–363, 2022.'
ista: 'Zabelkin A, Yakovleva Y, Bochkareva O, Alexeev N. 2022. PaReBrick: PArallel
REarrangements and BReaks identification toolkit. Bioinformatics. 38(2), 357–363.'
mla: 'Zabelkin, Alexey, et al. “PaReBrick: PArallel REarrangements and BReaks Identification
Toolkit.” Bioinformatics, vol. 38, no. 2, Oxford Academic, 2022, pp. 357–63,
doi:10.1093/bioinformatics/btab691.'
short: A. Zabelkin, Y. Yakovleva, O. Bochkareva, N. Alexeev, Bioinformatics 38 (2022)
357–363.
date_created: 2022-03-27T22:01:46Z
date_published: 2022-01-15T00:00:00Z
date_updated: 2023-08-03T06:21:46Z
day: '15'
ddc:
- '000'
department:
- _id: FyKo
doi: 10.1093/bioinformatics/btab691
ec_funded: 1
external_id:
isi:
- '000743380100008'
file:
- access_level: open_access
checksum: 4b5688ff9ac86180ccdf7f82fa33d926
content_type: application/pdf
creator: dernst
date_created: 2022-03-28T08:07:46Z
date_updated: 2022-03-28T08:07:46Z
file_id: '10930'
file_name: 2022_Bioinformatics_Zabelkin.pdf
file_size: 3425744
relation: main_file
success: 1
file_date_updated: 2022-03-28T08:07:46Z
has_accepted_license: '1'
intvolume: ' 38'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 357-363
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Bioinformatics
publication_identifier:
eissn:
- 1460-2059
issn:
- 1367-4803
publication_status: published
publisher: Oxford Academic
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/ctlab/parallel-rearrangements
scopus_import: '1'
status: public
title: 'PaReBrick: PArallel REarrangements and BReaks identification toolkit'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 38
year: '2022'
...
---
_id: '11187'
abstract:
- lang: eng
text: During the COVID-19 pandemic, genomics and bioinformatics have emerged as
essential public health tools. The genomic data acquired using these methods have
supported the global health response, facilitated the development of testing methods
and allowed the timely tracking of novel SARS-CoV-2 variants. Yet the virtually
unlimited potential for rapid generation and analysis of genomic data is also
coupled with unique technical, scientific and organizational challenges. Here,
we discuss the application of genomic and computational methods for efficient
data-driven COVID-19 response, the advantages of the democratization of viral
sequencing around the world and the challenges associated with viral genome data
collection and processing.
acknowledgement: 'Our paper is dedicated to all freedom-loving people around the world,
and to the people of Ukraine who fight for our freedom. We thank William M. Switzer
and Ellsworth M. Campbell from the Division of HIV/AIDS Prevention, Centers for
Disease Control and Prevention (CDC), Atlanta, GA, USA, for discussions and suggestions.
We thank Jason Ladner from the Pathogen and Microbiome Institute, Northern Arizona
University, Flagstaff, AZ, for providing suggestions and feedback. S.M. was partially
supported by National Science Foundation grants 2041984. T.L. is supported by the
NSFC Excellent Young Scientists Fund (Hong Kong and Macau; 31922087), Research Grants
Council (RGC) Collaborative Research Fund (C7144-20GF), RGC Research Impact Fund
(R7021-20), Innovation and Technology Commission’s InnoHK funding (D24H) and Health
and Medical Research Fund (COVID190223). P.S. was supported by US National Institutes
of Health (NIH) grant 1R01EB025022 and National Science Foundation (NSF) grant 2047828.
M.A. acknowledges King Abdulaziz City for Science and Technology and the Saudi Human
Genome Project for technical and financial support (https://shgp.kacst.edu.sa) N.W.
was supported by US NIH grants R00 AI139445, DP2 AT011966 and R01 AI167910. A.S.
acknowledge funding from NSF grant no. 2029025. A.Z. has been partially supported
by NIH grants 1R01EB025022-01 and 1R21CA241044-01A1. S. Knyazev has been partly
supported by Molecular Basis of Disease at Georgia State University and NIH awards
R01 HG009120, R01 MH115676, R01 AI153827 and U01 HG011715. A.W. has been supported
by the CAMS Innovation Fund for Medical Sciences (2021-I2M-1-061). R.K. was supported
by NSF project 2038509, RAPID: Improving QIIME 2 and UniFrac for Viruses to Respond
to COVID-19, CDC project 30055281 with Scripps led by Kristian Andersen, Genomic
sequencing of SARS-CoV-2 to investigate local and cross-border emergence and spread.
J.O.W. was supported by NIH–National Institute of Allergy and Infectious Diseases
(NIAID) R01 AI135992 and receives funding from the CDC unrelated to this work. T.I.V.
is supported by the Branco Weiss Fellowship. Y.P. was supported by the Ministry
of Science and Higher Education of the Russian Federation within the framework of
state support for the creation and development of World-Class Research Centers “Digital
biodesign and personalized healthcare” N◦075-15-2020-926. E.B. was supported by
a US National Institute of General Medical Sciences IDeA Alaska INBRE (P20GM103395)
and NIAID CEIRR (75N93019R00028). C.E.M. thanks Testing for America (501c3), OpenCovidScreen
Foundation, Igor Tulchinsky and the WorldQuant Foundation, Bill Ackman and Olivia
Flatto and the Pershing Square Foundation, Ken Griffin and Citadel, the US National
Institutes of Health (R01AI125416, R01AI151059, R21AI129851, U01DA053941), and the
Alfred P. Sloan Foundation (G-2015-13964). C.Y.C. is supported by US CDC Epidemiology
and Laboratory Capacity (ELC) for Infectious Diseases grant 6NU50CK000539 to the
California Department of Public Health, the Innovative Genomics Institute (IGI)
at the University of California, Berkeley, and University of California, San Francisco,
NIH grant R33AI12945 and US CDC contract 75D30121C10991. A.K. was partly supported
by RFBR grant 20-515-80017. P.L. acknowledges support from the European Research
Council (ERC) under the European Union’s Horizon 2020 research and innovation program
(grant agreement no. ~725422 - ReservoirDOCS), the Wellcome Trust through project
206298/Z/17/Z (Artic Network) and NIH grants R01 AI153044 and U19 AI135995. K.C.
acknowledges support from the US NSF award EEID-IOS-2109688. F.K.’s work was supported
by an ERC Consolidator grant to F.K. (771209–CharFL).'
article_processing_charge: No
article_type: letter_note
author:
- first_name: Sergey
full_name: Knyazev, Sergey
last_name: Knyazev
- first_name: Karishma
full_name: Chhugani, Karishma
last_name: Chhugani
- first_name: Varuni
full_name: Sarwal, Varuni
last_name: Sarwal
- first_name: Ram
full_name: Ayyala, Ram
last_name: Ayyala
- first_name: Harman
full_name: Singh, Harman
last_name: Singh
- first_name: Smruthi
full_name: Karthikeyan, Smruthi
last_name: Karthikeyan
- first_name: Dhrithi
full_name: Deshpande, Dhrithi
last_name: Deshpande
- first_name: Pelin Icer
full_name: Baykal, Pelin Icer
last_name: Baykal
- first_name: Zoia
full_name: Comarova, Zoia
last_name: Comarova
- first_name: Angela
full_name: Lu, Angela
last_name: Lu
- first_name: Yuri
full_name: Porozov, Yuri
last_name: Porozov
- first_name: Tetyana I.
full_name: Vasylyeva, Tetyana I.
last_name: Vasylyeva
- first_name: Joel O.
full_name: Wertheim, Joel O.
last_name: Wertheim
- first_name: Braden T.
full_name: Tierney, Braden T.
last_name: Tierney
- first_name: Charles Y.
full_name: Chiu, Charles Y.
last_name: Chiu
- first_name: Ren
full_name: Sun, Ren
last_name: Sun
- first_name: Aiping
full_name: Wu, Aiping
last_name: Wu
- first_name: Malak S.
full_name: Abedalthagafi, Malak S.
last_name: Abedalthagafi
- first_name: Victoria M.
full_name: Pak, Victoria M.
last_name: Pak
- first_name: Shivashankar H.
full_name: Nagaraj, Shivashankar H.
last_name: Nagaraj
- first_name: Adam L.
full_name: Smith, Adam L.
last_name: Smith
- first_name: Pavel
full_name: Skums, Pavel
last_name: Skums
- first_name: Bogdan
full_name: Pasaniuc, Bogdan
last_name: Pasaniuc
- first_name: Andrey
full_name: Komissarov, Andrey
last_name: Komissarov
- first_name: Christopher E.
full_name: Mason, Christopher E.
last_name: Mason
- first_name: Eric
full_name: Bortz, Eric
last_name: Bortz
- first_name: Philippe
full_name: Lemey, Philippe
last_name: Lemey
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Niko
full_name: Beerenwinkel, Niko
last_name: Beerenwinkel
- first_name: Tommy Tsan Yuk
full_name: Lam, Tommy Tsan Yuk
last_name: Lam
- first_name: Nicholas C.
full_name: Wu, Nicholas C.
last_name: Wu
- first_name: Alex
full_name: Zelikovsky, Alex
last_name: Zelikovsky
- first_name: Rob
full_name: Knight, Rob
last_name: Knight
- first_name: Keith A.
full_name: Crandall, Keith A.
last_name: Crandall
- first_name: Serghei
full_name: Mangul, Serghei
last_name: Mangul
citation:
ama: Knyazev S, Chhugani K, Sarwal V, et al. Unlocking capacities of genomics for
the COVID-19 response and future pandemics. Nature Methods. 2022;19(4):374-380.
doi:10.1038/s41592-022-01444-z
apa: Knyazev, S., Chhugani, K., Sarwal, V., Ayyala, R., Singh, H., Karthikeyan,
S., … Mangul, S. (2022). Unlocking capacities of genomics for the COVID-19 response
and future pandemics. Nature Methods. Springer Nature. https://doi.org/10.1038/s41592-022-01444-z
chicago: Knyazev, Sergey, Karishma Chhugani, Varuni Sarwal, Ram Ayyala, Harman Singh,
Smruthi Karthikeyan, Dhrithi Deshpande, et al. “Unlocking Capacities of Genomics
for the COVID-19 Response and Future Pandemics.” Nature Methods. Springer
Nature, 2022. https://doi.org/10.1038/s41592-022-01444-z.
ieee: S. Knyazev et al., “Unlocking capacities of genomics for the COVID-19
response and future pandemics,” Nature Methods, vol. 19, no. 4. Springer
Nature, pp. 374–380, 2022.
ista: Knyazev S, Chhugani K, Sarwal V, Ayyala R, Singh H, Karthikeyan S, Deshpande
D, Baykal PI, Comarova Z, Lu A, Porozov Y, Vasylyeva TI, Wertheim JO, Tierney
BT, Chiu CY, Sun R, Wu A, Abedalthagafi MS, Pak VM, Nagaraj SH, Smith AL, Skums
P, Pasaniuc B, Komissarov A, Mason CE, Bortz E, Lemey P, Kondrashov F, Beerenwinkel
N, Lam TTY, Wu NC, Zelikovsky A, Knight R, Crandall KA, Mangul S. 2022. Unlocking
capacities of genomics for the COVID-19 response and future pandemics. Nature
Methods. 19(4), 374–380.
mla: Knyazev, Sergey, et al. “Unlocking Capacities of Genomics for the COVID-19
Response and Future Pandemics.” Nature Methods, vol. 19, no. 4, Springer
Nature, 2022, pp. 374–80, doi:10.1038/s41592-022-01444-z.
short: S. Knyazev, K. Chhugani, V. Sarwal, R. Ayyala, H. Singh, S. Karthikeyan,
D. Deshpande, P.I. Baykal, Z. Comarova, A. Lu, Y. Porozov, T.I. Vasylyeva, J.O.
Wertheim, B.T. Tierney, C.Y. Chiu, R. Sun, A. Wu, M.S. Abedalthagafi, V.M. Pak,
S.H. Nagaraj, A.L. Smith, P. Skums, B. Pasaniuc, A. Komissarov, C.E. Mason, E.
Bortz, P. Lemey, F. Kondrashov, N. Beerenwinkel, T.T.Y. Lam, N.C. Wu, A. Zelikovsky,
R. Knight, K.A. Crandall, S. Mangul, Nature Methods 19 (2022) 374–380.
date_created: 2022-04-17T22:01:48Z
date_published: 2022-04-08T00:00:00Z
date_updated: 2023-08-03T06:46:09Z
day: '08'
department:
- _id: FyKo
doi: 10.1038/s41592-022-01444-z
ec_funded: 1
external_id:
isi:
- '000781199600011'
pmid:
- '35396471'
intvolume: ' 19'
isi: 1
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41592-022-01444-z
month: '04'
oa: 1
oa_version: Published Version
page: 374-380
pmid: 1
project:
- _id: 26580278-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '771209'
name: Characterizing the fitness landscape on population and global scales
publication: Nature Methods
publication_identifier:
eissn:
- 1548-7105
issn:
- 1548-7091
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Unlocking capacities of genomics for the COVID-19 response and future pandemics
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 19
year: '2022'
...
---
_id: '11344'
abstract:
- lang: eng
text: Until recently, Shigella and enteroinvasive Escherichia coli were thought
to be primate-restricted pathogens. The base of their pathogenicity is the type
3 secretion system (T3SS) encoded by the pINV virulence plasmid, which facilitates
host cell invasion and subsequent proliferation. A large family of T3SS effectors,
E3 ubiquitin-ligases encoded by the ipaH genes, have a key role in the Shigella
pathogenicity through the modulation of cellular ubiquitination that degrades
host proteins. However, recent genomic studies identified ipaH genes in the genomes
of Escherichia marmotae, a potential marmot pathogen, and an E. coli extracted
from fecal samples of bovine calves, suggesting that non-human hosts may also
be infected by these strains, potentially pathogenic to humans. We performed a
comparative genomic study of the functional repertoires in the ipaH gene family
in Shigella and enteroinvasive Escherichia from human and predicted non-human
hosts. We found that fewer than half of Shigella genomes had a complete set of
ipaH genes, with frequent gene losses and duplications that were not consistent
with the species tree and nomenclature. Non-human host IpaH proteins had a diverse
set of substrate-binding domains and, in contrast to the Shigella proteins, two
variants of the NEL C-terminal domain. Inconsistencies between strains phylogeny
and composition of effectors indicate horizontal gene transfer between E. coli
adapted to different hosts. These results provide a framework for understanding
of ipaH-mediated host-pathogens interactions and suggest a need for a genomic
study of fecal samples from diseased animals.
acknowledgement: 'The project was initiated with Aygul Minnegalieva and Yulia Yakovleva
at the Summer School of Molecular and Theoretical Biology (SMTB-2020), supported
by the Zimin Foundation. We thank Inna Shapovalenko, Daria Abuzova, Elizaveta Kaminskaya,
and Dmitriy Zvezdin for their contribution to the project during SMTB-2020. We also
thank Peter Vlasov for fruitful discussions.This study was supported by the Russian
Foundation for Basic Research (RFBR), Grant # 20-54-14005 and Fonds zur Förderung
der wissenschaftlichen Forschung (FWF), Grant # I5127-B. The work of OB is supported
by the European Union’s Horizon 2020 Research and Innovation Programme under the
Marie Skłodowska-Curie Grant Agreement No. 754411. '
article_number: '6868'
article_processing_charge: No
article_type: original
author:
- first_name: NO
full_name: Dranenko, NO
last_name: Dranenko
- first_name: MN
full_name: Tutukina, MN
last_name: Tutukina
- first_name: MS
full_name: Gelfand, MS
last_name: Gelfand
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
citation:
ama: Dranenko N, Tutukina M, Gelfand M, Kondrashov F, Bochkareva O. Chromosome-encoded
IpaH ubiquitin ligases indicate non-human enteroinvasive Escherichia. Scientific
Reports. 2022;12. doi:10.1038/s41598-022-10827-3
apa: Dranenko, N., Tutukina, M., Gelfand, M., Kondrashov, F., & Bochkareva,
O. (2022). Chromosome-encoded IpaH ubiquitin ligases indicate non-human enteroinvasive
Escherichia. Scientific Reports. Springer Nature. https://doi.org/10.1038/s41598-022-10827-3
chicago: Dranenko, NO, MN Tutukina, MS Gelfand, Fyodor Kondrashov, and Olga Bochkareva.
“Chromosome-Encoded IpaH Ubiquitin Ligases Indicate Non-Human Enteroinvasive Escherichia.”
Scientific Reports. Springer Nature, 2022. https://doi.org/10.1038/s41598-022-10827-3.
ieee: N. Dranenko, M. Tutukina, M. Gelfand, F. Kondrashov, and O. Bochkareva, “Chromosome-encoded
IpaH ubiquitin ligases indicate non-human enteroinvasive Escherichia,” Scientific
Reports, vol. 12. Springer Nature, 2022.
ista: Dranenko N, Tutukina M, Gelfand M, Kondrashov F, Bochkareva O. 2022. Chromosome-encoded
IpaH ubiquitin ligases indicate non-human enteroinvasive Escherichia. Scientific
Reports. 12, 6868.
mla: Dranenko, NO, et al. “Chromosome-Encoded IpaH Ubiquitin Ligases Indicate Non-Human
Enteroinvasive Escherichia.” Scientific Reports, vol. 12, 6868, Springer
Nature, 2022, doi:10.1038/s41598-022-10827-3.
short: N. Dranenko, M. Tutukina, M. Gelfand, F. Kondrashov, O. Bochkareva, Scientific
Reports 12 (2022).
date_created: 2022-05-02T07:08:42Z
date_published: 2022-04-27T00:00:00Z
date_updated: 2023-08-03T06:59:49Z
day: '27'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1038/s41598-022-10827-3
ec_funded: 1
external_id:
isi:
- '000788639400032'
pmid:
- '35477739'
file:
- access_level: open_access
checksum: 12601b8a5c6b83bb618f92bcb963ecc9
content_type: application/pdf
creator: dernst
date_created: 2022-05-02T09:05:20Z
date_updated: 2022-05-02T09:05:20Z
file_id: '11349'
file_name: 2022_ScientificReports_Dranenko.pdf
file_size: 3564155
relation: main_file
success: 1
file_date_updated: 2022-05-02T09:05:20Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: c098eddd-5a5b-11eb-8a69-abe27170a68f
grant_number: I05127
name: Evolutionary analysis of gene regulation
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Scientific Reports
publication_identifier:
issn:
- 2045-2322
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Chromosome-encoded IpaH ubiquitin ligases indicate non-human enteroinvasive
Escherichia
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2022'
...
---
_id: '11448'
abstract:
- lang: eng
text: Studies of protein fitness landscapes reveal biophysical constraints guiding
protein evolution and empower prediction of functional proteins. However, generalisation
of these findings is limited due to scarceness of systematic data on fitness landscapes
of proteins with a defined evolutionary relationship. We characterized the fitness
peaks of four orthologous fluorescent proteins with a broad range of sequence
divergence. While two of the four studied fitness peaks were sharp, the other
two were considerably flatter, being almost entirely free of epistatic interactions.
Mutationally robust proteins, characterized by a flat fitness peak, were not optimal
templates for machine-learning-driven protein design – instead, predictions were
more accurate for fragile proteins with epistatic landscapes. Our work paves insights
for practical application of fitness landscape heterogeneity in protein engineering.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: "We thank Ondřej Draganov, Rodrigo Redondo, Bor Kavčič, Mia Juračić
and Andrea Pauli for discussion and technical advice. We thank Anita Testa Salmazo
for advice on resin protein purification, Dmitry Bolotin and the Milaboratory (milaboratory.com)
for access to computing and storage infrastructure, and Josef Houser and Eva Fujdiarova
for technical assistance and data interpretation. Core facility Biomolecular Interactions
and Crystallization of CEITEC Masaryk University is gratefully acknowledged for
the obtaining of the scientific data presented in this paper. This research was
supported by the Scientific Service Units (SSU) of IST-Austria\r\nthrough resources
provided by the Bioimaging Facility (BIF), and the Life Science Facility (LSF).
MiSeq and HiSeq NGS sequencing was performed by the Next Generation Sequencing Facility
at Vienna BioCenter Core Facilities (VBCF), member of the Vienna BioCenter (VBC),
Austria. FACS was performed at the BioOptics Facility of the Institute of Molecular
Pathology (IMP), Austria. We also thank the Biomolecular Crystallography Facility
in the Vanderbilt University Center for Structural Biology. We are grateful to Joel
M Harp for help with X-ray data collection. This work was supported by the ERC Consolidator
grant to FAK (771209—CharFL). KSS acknowledges support by President’s Grant МК–5405.2021.1.4,
the Imperial College Research Fellowship and the MRC London Institute of Medical
Sciences (UKRI MC-A658-5QEA0).\r\nAF is supported by the Marie Skłodowska-Curie
Fellowship (H2020-MSCA-IF-2019, Grant Agreement No. 898203, Project acronym \"FLINDIP\").
Experiments were partially carried out using equipment provided by the Institute
of Bioorganic Chemistry of the Russian Academy of Sciences Сore Facility (CKP IBCH).
This work was supported by a Russian Science Foundation grant 19-74-10102.This project
has received funding from the European Union’s Horizon 2020 research and innovation
programme under the Marie Skłodowska-Curie Grant Agreement No. 665,385."
article_number: '75842'
article_processing_charge: No
article_type: original
author:
- first_name: Louisa
full_name: Gonzalez Somermeyer, Louisa
id: 4720D23C-F248-11E8-B48F-1D18A9856A87
last_name: Gonzalez Somermeyer
orcid: 0000-0001-9139-5383
- first_name: Aubin
full_name: Fleiss, Aubin
last_name: Fleiss
- first_name: Alexander S
full_name: Mishin, Alexander S
last_name: Mishin
- first_name: Nina G
full_name: Bozhanova, Nina G
last_name: Bozhanova
- first_name: Anna A
full_name: Igolkina, Anna A
last_name: Igolkina
- first_name: Jens
full_name: Meiler, Jens
last_name: Meiler
- first_name: Maria-Elisenda
full_name: Alaball Pujol, Maria-Elisenda
last_name: Alaball Pujol
- first_name: Ekaterina V
full_name: Putintseva, Ekaterina V
last_name: Putintseva
- first_name: Karen S
full_name: Sarkisyan, Karen S
last_name: Sarkisyan
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
citation:
ama: Gonzalez Somermeyer L, Fleiss A, Mishin AS, et al. Heterogeneity of the GFP
fitness landscape and data-driven protein design. eLife. 2022;11. doi:10.7554/elife.75842
apa: Gonzalez Somermeyer, L., Fleiss, A., Mishin, A. S., Bozhanova, N. G., Igolkina,
A. A., Meiler, J., … Kondrashov, F. (2022). Heterogeneity of the GFP fitness landscape
and data-driven protein design. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.75842
chicago: Gonzalez Somermeyer, Louisa, Aubin Fleiss, Alexander S Mishin, Nina G Bozhanova,
Anna A Igolkina, Jens Meiler, Maria-Elisenda Alaball Pujol, Ekaterina V Putintseva,
Karen S Sarkisyan, and Fyodor Kondrashov. “Heterogeneity of the GFP Fitness Landscape
and Data-Driven Protein Design.” ELife. eLife Sciences Publications, 2022.
https://doi.org/10.7554/elife.75842.
ieee: L. Gonzalez Somermeyer et al., “Heterogeneity of the GFP fitness landscape
and data-driven protein design,” eLife, vol. 11. eLife Sciences Publications,
2022.
ista: Gonzalez Somermeyer L, Fleiss A, Mishin AS, Bozhanova NG, Igolkina AA, Meiler
J, Alaball Pujol M-E, Putintseva EV, Sarkisyan KS, Kondrashov F. 2022. Heterogeneity
of the GFP fitness landscape and data-driven protein design. eLife. 11, 75842.
mla: Gonzalez Somermeyer, Louisa, et al. “Heterogeneity of the GFP Fitness Landscape
and Data-Driven Protein Design.” ELife, vol. 11, 75842, eLife Sciences
Publications, 2022, doi:10.7554/elife.75842.
short: L. Gonzalez Somermeyer, A. Fleiss, A.S. Mishin, N.G. Bozhanova, A.A. Igolkina,
J. Meiler, M.-E. Alaball Pujol, E.V. Putintseva, K.S. Sarkisyan, F. Kondrashov,
ELife 11 (2022).
date_created: 2022-06-18T09:06:59Z
date_published: 2022-05-05T00:00:00Z
date_updated: 2023-08-03T07:20:15Z
day: '05'
ddc:
- '570'
department:
- _id: GradSch
- _id: FyKo
doi: 10.7554/elife.75842
ec_funded: 1
external_id:
isi:
- '000799197200001'
file:
- access_level: open_access
checksum: 7573c28f44028ab0cc81faef30039e44
content_type: application/pdf
creator: dernst
date_created: 2022-06-20T07:44:19Z
date_updated: 2022-06-20T07:44:19Z
file_id: '11454'
file_name: 2022_eLife_Somermeyer.pdf
file_size: 5297213
relation: main_file
success: 1
file_date_updated: 2022-06-20T07:44:19Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
project:
- _id: 26580278-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '771209'
name: Characterizing the fitness landscape on population and global scales
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Heterogeneity of the GFP fitness landscape and data-driven protein design
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2022'
...
---
_id: '11587'
abstract:
- lang: eng
text: "Background: Accurate and comprehensive annotation of transcript sequences
is essential for transcript quantification and differential gene and transcript
expression analysis. Single-molecule long-read sequencing technologies provide
improved integrity of transcript structures including alternative splicing, and
transcription start and polyadenylation sites. However, accuracy is significantly
affected by sequencing errors, mRNA degradation, or incomplete cDNA synthesis.\r\nResults:
We present a new and comprehensive Arabidopsis thaliana Reference Transcript Dataset
3 (AtRTD3). AtRTD3 contains over 169,000 transcripts—twice that of the best current
Arabidopsis transcriptome and including over 1500 novel genes. Seventy-eight percent
of transcripts are from Iso-seq with accurately defined splice junctions and transcription
start and end sites. We develop novel methods to determine splice junctions and
transcription start and end sites accurately. Mismatch profiles around splice
junctions provide a powerful feature to distinguish correct splice junctions and
remove false splice junctions. Stratified approaches identify high-confidence
transcription start and end sites and remove fragmentary transcripts due to degradation.
AtRTD3 is a major improvement over existing transcriptomes as demonstrated by
analysis of an Arabidopsis cold response RNA-seq time-series. AtRTD3 provides
higher resolution of transcript expression profiling and identifies cold-induced
differential transcription start and polyadenylation site usage.\r\nConclusions:
AtRTD3 is the most comprehensive Arabidopsis transcriptome currently. It improves
the precision of differential gene and transcript expression, differential alternative
splicing, and transcription start/end site usage analysis from RNA-seq data. The
novel methods for identifying accurate splice junctions and transcription start/end
sites are widely applicable and will improve single-molecule sequencing analysis
from any species."
acknowledgement: "This work was jointly supported by funding from the Biotechnology
and Biological Sciences Research Council (BBSRC) BB/P009751/1 to JB; BB/R014582/1
to RW and RZ; BB/S020160/1 to RZ; BB/S004610/1 (16 ERA-CAPS BARN) to RW; the Scottish
Government Rural and Environment Science and Analytical Services division (RESAS)
[to RZ, RW, and JB]; the\r\nNational Science Foundation (MCB-2014408) and the National
Institute of Health (NIH) (GM-114297) to E.H.; S. H. was supported by funding to
K.D. from the University of York; the Austrian Science Fund (FWF) SFB F43 to AB
and MJ and [P26333] to MK; The French Agence Nationale de la Recherche grant ANR-16-CE12-0032
to MC; the Japan Science and\r\nTechnology Agency (JST), the Core Research for Evolutionary
Science and Technology (CREST; Grant Number JPMJCR13B4) to M.S.; the National Science
Foundation (Grant No. DBI1949036 to A.b.H and A.S.N.R, and Grant No. MCB 2014542
to E.H. and A.S.N.R.); and the DOE Office of Science, Office of Biological and Environmental
Research (Grant\r\nNo. DE-SC0010733) to A.S.N.R and A.b.H.; the Deutsche Forschungsgemeinschaft
(DFG) STA653/14-1 and STA653/15-1 to DS; the National Science Foundation grant (IOS-154173)
to Q.Q.L.; the German Research Foundation (DFG) WA2167/8-1 to AW and SFB1101/C03
to AW and TWK; the Research Grants Council (RGC) of Hong Kong (GRF 12103020) to
LX. NSF grant IOS-1849708 and NSF EPSCoR grant 1826836 to RS; the Academia Sinica
to S.-L. T."
article_number: '149'
article_processing_charge: No
article_type: original
author:
- first_name: Runxuan
full_name: Zhang, Runxuan
last_name: Zhang
- first_name: Richard
full_name: Kuo, Richard
last_name: Kuo
- first_name: Max
full_name: Coulter, Max
last_name: Coulter
- first_name: Cristiane P.G.
full_name: Calixto, Cristiane P.G.
last_name: Calixto
- first_name: Juan Carlos
full_name: Entizne, Juan Carlos
last_name: Entizne
- first_name: Wenbin
full_name: Guo, Wenbin
last_name: Guo
- first_name: Yamile
full_name: Marquez, Yamile
last_name: Marquez
- first_name: Linda
full_name: Milne, Linda
last_name: Milne
- first_name: Stefan
full_name: Riegler, Stefan
id: FF6018E0-D806-11E9-8E43-0B14E6697425
last_name: Riegler
orcid: 0000-0003-3413-1343
- first_name: Akihiro
full_name: Matsui, Akihiro
last_name: Matsui
- first_name: Maho
full_name: Tanaka, Maho
last_name: Tanaka
- first_name: Sarah
full_name: Harvey, Sarah
last_name: Harvey
- first_name: Yubang
full_name: Gao, Yubang
last_name: Gao
- first_name: Theresa
full_name: Wießner-Kroh, Theresa
last_name: Wießner-Kroh
- first_name: Alejandro
full_name: Paniagua, Alejandro
last_name: Paniagua
- first_name: Martin
full_name: Crespi, Martin
last_name: Crespi
- first_name: Katherine
full_name: Denby, Katherine
last_name: Denby
- first_name: Asa Ben
full_name: Hur, Asa Ben
last_name: Hur
- first_name: Enamul
full_name: Huq, Enamul
last_name: Huq
- first_name: Michael
full_name: Jantsch, Michael
last_name: Jantsch
- first_name: Artur
full_name: Jarmolowski, Artur
last_name: Jarmolowski
- first_name: Tino
full_name: Koester, Tino
last_name: Koester
- first_name: Sascha
full_name: Laubinger, Sascha
last_name: Laubinger
- first_name: Qingshun Quinn
full_name: Li, Qingshun Quinn
last_name: Li
- first_name: Lianfeng
full_name: Gu, Lianfeng
last_name: Gu
- first_name: Motoaki
full_name: Seki, Motoaki
last_name: Seki
- first_name: Dorothee
full_name: Staiger, Dorothee
last_name: Staiger
- first_name: Ramanjulu
full_name: Sunkar, Ramanjulu
last_name: Sunkar
- first_name: Zofia
full_name: Szweykowska-Kulinska, Zofia
last_name: Szweykowska-Kulinska
- first_name: Shih Long
full_name: Tu, Shih Long
last_name: Tu
- first_name: Andreas
full_name: Wachter, Andreas
last_name: Wachter
- first_name: Robbie
full_name: Waugh, Robbie
last_name: Waugh
- first_name: Liming
full_name: Xiong, Liming
last_name: Xiong
- first_name: Xiao Ning
full_name: Zhang, Xiao Ning
last_name: Zhang
- first_name: Ana
full_name: Conesa, Ana
last_name: Conesa
- first_name: Anireddy S.N.
full_name: Reddy, Anireddy S.N.
last_name: Reddy
- first_name: Andrea
full_name: Barta, Andrea
last_name: Barta
- first_name: Maria
full_name: Kalyna, Maria
last_name: Kalyna
- first_name: John W.S.
full_name: Brown, John W.S.
last_name: Brown
citation:
ama: Zhang R, Kuo R, Coulter M, et al. A high-resolution single-molecule sequencing-based
Arabidopsis transcriptome using novel methods of Iso-seq analysis. Genome Biology.
2022;23. doi:10.1186/s13059-022-02711-0
apa: Zhang, R., Kuo, R., Coulter, M., Calixto, C. P. G., Entizne, J. C., Guo, W.,
… Brown, J. W. S. (2022). A high-resolution single-molecule sequencing-based Arabidopsis
transcriptome using novel methods of Iso-seq analysis. Genome Biology.
BioMed Central. https://doi.org/10.1186/s13059-022-02711-0
chicago: Zhang, Runxuan, Richard Kuo, Max Coulter, Cristiane P.G. Calixto, Juan
Carlos Entizne, Wenbin Guo, Yamile Marquez, et al. “A High-Resolution Single-Molecule
Sequencing-Based Arabidopsis Transcriptome Using Novel Methods of Iso-Seq Analysis.”
Genome Biology. BioMed Central, 2022. https://doi.org/10.1186/s13059-022-02711-0.
ieee: R. Zhang et al., “A high-resolution single-molecule sequencing-based
Arabidopsis transcriptome using novel methods of Iso-seq analysis,” Genome
Biology, vol. 23. BioMed Central, 2022.
ista: Zhang R, Kuo R, Coulter M, Calixto CPG, Entizne JC, Guo W, Marquez Y, Milne
L, Riegler S, Matsui A, Tanaka M, Harvey S, Gao Y, Wießner-Kroh T, Paniagua A,
Crespi M, Denby K, Hur AB, Huq E, Jantsch M, Jarmolowski A, Koester T, Laubinger
S, Li QQ, Gu L, Seki M, Staiger D, Sunkar R, Szweykowska-Kulinska Z, Tu SL, Wachter
A, Waugh R, Xiong L, Zhang XN, Conesa A, Reddy ASN, Barta A, Kalyna M, Brown JWS.
2022. A high-resolution single-molecule sequencing-based Arabidopsis transcriptome
using novel methods of Iso-seq analysis. Genome Biology. 23, 149.
mla: Zhang, Runxuan, et al. “A High-Resolution Single-Molecule Sequencing-Based
Arabidopsis Transcriptome Using Novel Methods of Iso-Seq Analysis.” Genome
Biology, vol. 23, 149, BioMed Central, 2022, doi:10.1186/s13059-022-02711-0.
short: R. Zhang, R. Kuo, M. Coulter, C.P.G. Calixto, J.C. Entizne, W. Guo, Y. Marquez,
L. Milne, S. Riegler, A. Matsui, M. Tanaka, S. Harvey, Y. Gao, T. Wießner-Kroh,
A. Paniagua, M. Crespi, K. Denby, A.B. Hur, E. Huq, M. Jantsch, A. Jarmolowski,
T. Koester, S. Laubinger, Q.Q. Li, L. Gu, M. Seki, D. Staiger, R. Sunkar, Z. Szweykowska-Kulinska,
S.L. Tu, A. Wachter, R. Waugh, L. Xiong, X.N. Zhang, A. Conesa, A.S.N. Reddy,
A. Barta, M. Kalyna, J.W.S. Brown, Genome Biology 23 (2022).
date_created: 2022-07-17T22:01:53Z
date_published: 2022-07-07T00:00:00Z
date_updated: 2023-08-03T12:04:18Z
day: '07'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1186/s13059-022-02711-0
external_id:
isi:
- '000821915500002'
file:
- access_level: open_access
checksum: 2c30ef84151d257a6b835b4e069b70ac
content_type: application/pdf
creator: dernst
date_created: 2022-07-18T08:15:24Z
date_updated: 2022-07-18T08:15:24Z
file_id: '11597'
file_name: 2022_GenomeBiology_Zhang.pdf
file_size: 3146207
relation: main_file
success: 1
file_date_updated: 2022-07-18T08:15:24Z
has_accepted_license: '1'
intvolume: ' 23'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
publication: Genome Biology
publication_identifier:
eissn:
- 1474-760X
publication_status: published
publisher: BioMed Central
quality_controlled: '1'
scopus_import: '1'
status: public
title: A high-resolution single-molecule sequencing-based Arabidopsis transcriptome
using novel methods of Iso-seq analysis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 23
year: '2022'
...
---
_id: '12131'
abstract:
- lang: eng
text: Replication-incompetent adenoviral vectors have been extensively used as a
platform for vaccine design, with at least four anti-COVID-19 vaccines authorized
to date. These vaccines elicit neutralizing antibody responses directed against
SARS-CoV-2 Spike protein and confer significant level of protection against SARS-CoV-2
infection. Immunization with adenovirus-vectored vaccines is known to be accompanied
by the production of anti-vector antibodies, which may translate into reduced
efficacy of booster or repeated rounds of revaccination. Here, we used blood samples
from patients who received an adenovirus-based Gam-COVID-Vac vaccine to address
the question of whether anti-vector antibodies may influence the magnitude of
SARS-CoV-2-specific humoral response after booster vaccination. We observed that
rAd26-based prime vaccination with Gam-COVID-Vac induced the development of Ad26-neutralizing
antibodies, which persisted in circulation for at least 9 months. Our analysis
further indicates that high pre-boost Ad26 neutralizing antibody titers do not
appear to affect the humoral immunogenicity of the Gam-COVID-Vac boost. The titers
of anti-SARS-CoV-2 RBD IgGs and antibodies, which neutralized both the wild type
and the circulating variants of concern of SARS-CoV-2 such as Delta and Omicron,
were independent of the pre-boost levels of Ad26-neutralizing antibodies. Thus,
our results support the development of repeated immunization schedule with adenovirus-based
COVID-19 vaccines.
acknowledgement: We thank Sergey Kulemzin, Grigory Efimov, Yuri Lebedin, Alexander
Taranin and Rudolf Valenta for providing reagents. Figures were created with the
help of BioRender.com. This work was supported by the Russian Science Foundation
(Project 21-15-00286). Byazrova M.G. was supported by the RUDN University Strategic
Academic Leadership Program.
article_number: '145'
article_processing_charge: No
article_type: original
author:
- first_name: Maria G.
full_name: Byazrova, Maria G.
last_name: Byazrova
- first_name: Ekaterina A.
full_name: Astakhova, Ekaterina A.
last_name: Astakhova
- first_name: Aygul
full_name: Minnegalieva, Aygul
id: 87DF77F0-1D9A-11EA-B6AE-CE443DDC885E
last_name: Minnegalieva
- first_name: Maria M.
full_name: Sukhova, Maria M.
last_name: Sukhova
- first_name: Artem A.
full_name: Mikhailov, Artem A.
last_name: Mikhailov
- first_name: Alexey G.
full_name: Prilipov, Alexey G.
last_name: Prilipov
- first_name: Andrey A.
full_name: Gorchakov, Andrey A.
last_name: Gorchakov
- first_name: Alexander V.
full_name: Filatov, Alexander V.
last_name: Filatov
citation:
ama: Byazrova MG, Astakhova EA, Minnegalieva A, et al. Anti-Ad26 humoral immunity
does not compromise SARS-COV-2 neutralizing antibody responses following Gam-COVID-Vac
booster vaccination. npj Vaccines. 2022;7. doi:10.1038/s41541-022-00566-x
apa: Byazrova, M. G., Astakhova, E. A., Minnegalieva, A., Sukhova, M. M., Mikhailov,
A. A., Prilipov, A. G., … Filatov, A. V. (2022). Anti-Ad26 humoral immunity does
not compromise SARS-COV-2 neutralizing antibody responses following Gam-COVID-Vac
booster vaccination. Npj Vaccines. Springer Nature. https://doi.org/10.1038/s41541-022-00566-x
chicago: Byazrova, Maria G., Ekaterina A. Astakhova, Aygul Minnegalieva, Maria M.
Sukhova, Artem A. Mikhailov, Alexey G. Prilipov, Andrey A. Gorchakov, and Alexander
V. Filatov. “Anti-Ad26 Humoral Immunity Does Not Compromise SARS-COV-2 Neutralizing
Antibody Responses Following Gam-COVID-Vac Booster Vaccination.” Npj Vaccines.
Springer Nature, 2022. https://doi.org/10.1038/s41541-022-00566-x.
ieee: M. G. Byazrova et al., “Anti-Ad26 humoral immunity does not compromise
SARS-COV-2 neutralizing antibody responses following Gam-COVID-Vac booster vaccination,”
npj Vaccines, vol. 7. Springer Nature, 2022.
ista: Byazrova MG, Astakhova EA, Minnegalieva A, Sukhova MM, Mikhailov AA, Prilipov
AG, Gorchakov AA, Filatov AV. 2022. Anti-Ad26 humoral immunity does not compromise
SARS-COV-2 neutralizing antibody responses following Gam-COVID-Vac booster vaccination.
npj Vaccines. 7, 145.
mla: Byazrova, Maria G., et al. “Anti-Ad26 Humoral Immunity Does Not Compromise
SARS-COV-2 Neutralizing Antibody Responses Following Gam-COVID-Vac Booster Vaccination.”
Npj Vaccines, vol. 7, 145, Springer Nature, 2022, doi:10.1038/s41541-022-00566-x.
short: M.G. Byazrova, E.A. Astakhova, A. Minnegalieva, M.M. Sukhova, A.A. Mikhailov,
A.G. Prilipov, A.A. Gorchakov, A.V. Filatov, Npj Vaccines 7 (2022).
date_created: 2023-01-12T12:02:54Z
date_published: 2022-11-15T00:00:00Z
date_updated: 2023-08-04T08:52:40Z
day: '15'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1038/s41541-022-00566-x
external_id:
isi:
- '000884278600004'
pmid:
- '36379998'
file:
- access_level: open_access
checksum: ddaac096381565b2b4b7dcc34cdbc4ee
content_type: application/pdf
creator: dernst
date_created: 2023-01-23T11:22:09Z
date_updated: 2023-01-23T11:22:09Z
file_id: '12347'
file_name: 2022_njpVaccines_Byazrova.pdf
file_size: 1856046
relation: main_file
success: 1
file_date_updated: 2023-01-23T11:22:09Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
keyword:
- Pharmacology (medical)
- Infectious Diseases
- Pharmacology
- Immunology
- SARS-COV-2
- COVID
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: npj Vaccines
publication_identifier:
issn:
- 2059-0105
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Anti-Ad26 humoral immunity does not compromise SARS-COV-2 neutralizing antibody
responses following Gam-COVID-Vac booster vaccination
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 7
year: '2022'
...
---
_id: '12173'
abstract:
- lang: eng
text: With increasing urbanization and industrialization, the prevalence of inflammatory
bowel diseases (IBDs) has steadily been rising over the past two decades. IBD
involves flares of gastrointestinal (GI) inflammation accompanied by microbiota
perturbations. However, microbial mechanisms that trigger such flares remain elusive.
Here, we analyzed the association of the emerging pathogen atypical enteropathogenic
E. coli (aEPEC) with IBD disease activity. The presence of diarrheagenic E. coli
was assessed in stool samples from 630 IBD patients and 234 age- and sex-matched
controls without GI symptoms. Microbiota was analyzed with 16S ribosomal RNA gene
amplicon sequencing, and 57 clinical aEPEC isolates were subjected to whole-genome
sequencing and in vitro pathogenicity experiments including biofilm formation,
epithelial barrier function and the ability to induce pro-inflammatory signaling.
The presence of aEPEC correlated with laboratory, clinical and endoscopic disease
activity in ulcerative colitis (UC), as well as microbiota dysbiosis. In vitro,
aEPEC strains induce epithelial p21-activated kinases, disrupt the epithelial
barrier and display potent biofilm formation. The effector proteins espV and espG2
distinguish aEPEC cultured from UC and Crohn’s disease patients, respectively.
EspV-positive aEPEC harbor more virulence factors and have a higher pro-inflammatory
potential, which is counteracted by 5-ASA. aEPEC may tip a fragile immune–microbiota
homeostasis and thereby contribute to flares in UC. aEPEC isolates from UC patients
display properties to disrupt the epithelial barrier and to induce pro-inflammatory
signaling in vitro.
acknowledgement: "We would like to acknowledge Anita Krnjic, Christina Gmainer, Marion
Nehr, Helga Mock, and Sena Ecin for technical support in conducting the experiments.\r\nThis
study was supported by the Austrian Science Fund (P 32302) and the Vienna Science
and Technology Fund (LS18- 053; Austrian Science Fund (FWF)) [P 32302]."
article_number: e2143218
article_processing_charge: No
article_type: original
author:
- first_name: Maximilian
full_name: Baumgartner, Maximilian
last_name: Baumgartner
- first_name: Rebecca
full_name: Zirnbauer, Rebecca
last_name: Zirnbauer
- first_name: Sabine
full_name: Schlager, Sabine
last_name: Schlager
- first_name: Daniel
full_name: Mertens, Daniel
last_name: Mertens
- first_name: Nikolaus
full_name: Gasche, Nikolaus
last_name: Gasche
- first_name: Barbara
full_name: Sladek, Barbara
last_name: Sladek
- first_name: Craig
full_name: Herbold, Craig
last_name: Herbold
- first_name: Olga
full_name: Bochkareva, Olga
last_name: Bochkareva
- first_name: Vera
full_name: Emelianenko, Vera
id: 20152b9d-927a-11ed-8107-be36d740812d
last_name: Emelianenko
- first_name: Harald
full_name: Vogelsang, Harald
last_name: Vogelsang
- first_name: Michaela
full_name: Lang, Michaela
last_name: Lang
- first_name: Anton
full_name: Klotz, Anton
last_name: Klotz
- first_name: Birgit
full_name: Moik, Birgit
last_name: Moik
- first_name: Athanasios
full_name: Makristathis, Athanasios
last_name: Makristathis
- first_name: David
full_name: Berry, David
last_name: Berry
- first_name: Stefanie
full_name: Dabsch, Stefanie
last_name: Dabsch
- first_name: Vineeta
full_name: Khare, Vineeta
last_name: Khare
- first_name: Christoph
full_name: Gasche, Christoph
last_name: Gasche
citation:
ama: Baumgartner M, Zirnbauer R, Schlager S, et al. Atypical enteropathogenic E.
coli are associated with disease activity in ulcerative colitis. Gut Microbes.
2022;14(1). doi:10.1080/19490976.2022.2143218
apa: Baumgartner, M., Zirnbauer, R., Schlager, S., Mertens, D., Gasche, N., Sladek,
B., … Gasche, C. (2022). Atypical enteropathogenic E. coli are associated with
disease activity in ulcerative colitis. Gut Microbes. Taylor & Francis.
https://doi.org/10.1080/19490976.2022.2143218
chicago: Baumgartner, Maximilian, Rebecca Zirnbauer, Sabine Schlager, Daniel Mertens,
Nikolaus Gasche, Barbara Sladek, Craig Herbold, et al. “Atypical Enteropathogenic
E. Coli Are Associated with Disease Activity in Ulcerative Colitis.” Gut Microbes.
Taylor & Francis, 2022. https://doi.org/10.1080/19490976.2022.2143218.
ieee: M. Baumgartner et al., “Atypical enteropathogenic E. coli are associated
with disease activity in ulcerative colitis,” Gut Microbes, vol. 14, no.
1. Taylor & Francis, 2022.
ista: Baumgartner M, Zirnbauer R, Schlager S, Mertens D, Gasche N, Sladek B, Herbold
C, Bochkareva O, Emelianenko V, Vogelsang H, Lang M, Klotz A, Moik B, Makristathis
A, Berry D, Dabsch S, Khare V, Gasche C. 2022. Atypical enteropathogenic E. coli
are associated with disease activity in ulcerative colitis. Gut Microbes. 14(1),
e2143218.
mla: Baumgartner, Maximilian, et al. “Atypical Enteropathogenic E. Coli Are Associated
with Disease Activity in Ulcerative Colitis.” Gut Microbes, vol. 14, no.
1, e2143218, Taylor & Francis, 2022, doi:10.1080/19490976.2022.2143218.
short: M. Baumgartner, R. Zirnbauer, S. Schlager, D. Mertens, N. Gasche, B. Sladek,
C. Herbold, O. Bochkareva, V. Emelianenko, H. Vogelsang, M. Lang, A. Klotz, B.
Moik, A. Makristathis, D. Berry, S. Dabsch, V. Khare, C. Gasche, Gut Microbes
14 (2022).
date_created: 2023-01-12T12:11:36Z
date_published: 2022-11-22T00:00:00Z
date_updated: 2023-08-04T09:10:18Z
day: '22'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1080/19490976.2022.2143218
external_id:
isi:
- '000889180100001'
file:
- access_level: open_access
checksum: ee7681a17ae27645e9b5c1df61c15429
content_type: application/pdf
creator: dernst
date_created: 2023-01-26T10:56:51Z
date_updated: 2023-01-26T10:56:51Z
file_id: '12400'
file_name: 2022_GutMicrobes_Baumgartner.pdf
file_size: 4075251
relation: main_file
success: 1
file_date_updated: 2023-01-26T10:56:51Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
issue: '1'
keyword:
- Infectious Diseases
- Microbiology (medical)
- Gastroenterology
- Microbiology
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Gut Microbes
publication_identifier:
eissn:
- 1949-0984
issn:
- 1949-0976
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
scopus_import: '1'
status: public
title: Atypical enteropathogenic E. coli are associated with disease activity in ulcerative
colitis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 14
year: '2022'
...
---
_id: '12116'
abstract:
- lang: eng
text: Russia’s unprovoked attack on Ukraine has destroyed civilian infrastructure,
including universities, research centers, and other academic infrastructure (1).
Many Ukrainian scholars and researchers remain in Ukraine, and their work has
suffered from major setbacks (2–4). We call on international scientists and institutions
to support them.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Karishma
full_name: Chhugani, Karishma
last_name: Chhugani
- first_name: Alina
full_name: Frolova, Alina
last_name: Frolova
- first_name: Yuriy
full_name: Salyha, Yuriy
last_name: Salyha
- first_name: Andrada
full_name: Fiscutean, Andrada
last_name: Fiscutean
- first_name: Oksana
full_name: Zlenko, Oksana
last_name: Zlenko
- first_name: Sanita
full_name: Reinsone, Sanita
last_name: Reinsone
- first_name: Walter W.
full_name: Wolfsberger, Walter W.
last_name: Wolfsberger
- first_name: Oleksandra V.
full_name: Ivashchenko, Oleksandra V.
last_name: Ivashchenko
- first_name: Megi
full_name: Maci, Megi
last_name: Maci
- first_name: Dmytro
full_name: Dziuba, Dmytro
last_name: Dziuba
- first_name: Andrii
full_name: Parkhomenko, Andrii
last_name: Parkhomenko
- first_name: Eric
full_name: Bortz, Eric
last_name: Bortz
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Paweł P.
full_name: Łabaj, Paweł P.
last_name: Łabaj
- first_name: Veronika
full_name: Romero, Veronika
last_name: Romero
- first_name: Jakub
full_name: Hlávka, Jakub
last_name: Hlávka
- first_name: Taras K.
full_name: Oleksyk, Taras K.
last_name: Oleksyk
- first_name: Serghei
full_name: Mangul, Serghei
last_name: Mangul
citation:
ama: Chhugani K, Frolova A, Salyha Y, et al. Remote opportunities for scholars in
Ukraine. Science. 2022;378(6626):1285-1286. doi:10.1126/science.adg0797
apa: Chhugani, K., Frolova, A., Salyha, Y., Fiscutean, A., Zlenko, O., Reinsone,
S., … Mangul, S. (2022). Remote opportunities for scholars in Ukraine. Science.
American Association for the Advancement of Science. https://doi.org/10.1126/science.adg0797
chicago: Chhugani, Karishma, Alina Frolova, Yuriy Salyha, Andrada Fiscutean, Oksana
Zlenko, Sanita Reinsone, Walter W. Wolfsberger, et al. “Remote Opportunities for
Scholars in Ukraine.” Science. American Association for the Advancement
of Science, 2022. https://doi.org/10.1126/science.adg0797.
ieee: K. Chhugani et al., “Remote opportunities for scholars in Ukraine,”
Science, vol. 378, no. 6626. American Association for the Advancement of
Science, pp. 1285–1286, 2022.
ista: Chhugani K, Frolova A, Salyha Y, Fiscutean A, Zlenko O, Reinsone S, Wolfsberger
WW, Ivashchenko OV, Maci M, Dziuba D, Parkhomenko A, Bortz E, Kondrashov F, Łabaj
PP, Romero V, Hlávka J, Oleksyk TK, Mangul S. 2022. Remote opportunities for scholars
in Ukraine. Science. 378(6626), 1285–1286.
mla: Chhugani, Karishma, et al. “Remote Opportunities for Scholars in Ukraine.”
Science, vol. 378, no. 6626, American Association for the Advancement of
Science, 2022, pp. 1285–86, doi:10.1126/science.adg0797.
short: K. Chhugani, A. Frolova, Y. Salyha, A. Fiscutean, O. Zlenko, S. Reinsone,
W.W. Wolfsberger, O.V. Ivashchenko, M. Maci, D. Dziuba, A. Parkhomenko, E. Bortz,
F. Kondrashov, P.P. Łabaj, V. Romero, J. Hlávka, T.K. Oleksyk, S. Mangul, Science
378 (2022) 1285–1286.
date_created: 2023-01-12T11:56:30Z
date_published: 2022-12-22T00:00:00Z
date_updated: 2023-10-03T11:01:06Z
day: '22'
department:
- _id: FyKo
doi: 10.1126/science.adg0797
external_id:
isi:
- '000963463700023'
intvolume: ' 378'
isi: 1
issue: '6626'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1126/science.adg0797
month: '12'
oa: 1
oa_version: Published Version
page: 1285-1286
publication: Science
publication_identifier:
eissn:
- 1095-9203
issn:
- 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Remote opportunities for scholars in Ukraine
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 378
year: '2022'
...
---
_id: '9255'
abstract:
- lang: eng
text: Our ability to trust that a random number is truly random is essential for
fields as diverse as cryptography and fundamental tests of quantum mechanics.
Existing solutions both come with drawbacks—device-independent quantum random
number generators (QRNGs) are highly impractical and standard semi-device-independent
QRNGs are limited to a specific physical implementation and level of trust. Here
we propose a framework for semi-device-independent randomness certification, using
a source of trusted vacuum in the form of a signal shutter. It employs a flexible
set of assumptions and levels of trust, allowing it to be applied in a wide range
of physical scenarios involving both quantum and classical entropy sources. We
experimentally demonstrate our protocol with a photonic setup and generate secure
random bits under three different assumptions with varying degrees of security
and resulting data rates.
acknowledgement: We would like to thank Robert Fickler for discussions about the experimental
realization and Marek Sýs for running the NIST randomness test on the data we acquired
in the experiment. We would like to thank Ugo Zanforlin, Gerald Buller, Daniel White,
and Cristian Bonato for their help with the experiment. M. Pivoluska, M. Plesch,
and M.M. acknowledge Czech-Austrian project MultiQUEST (I3053-N27 and GF17-33780L).
M. Pivoluska and M. Plesch additionally acknowledge the support of VEGA project
2/0136/19. M.F. acknowledges support from the Polish NCN grant Sonata UMO-2014/14/E/ST2/00020,
the European Research Council (ERC) under the European Union’s Horizon 2020 research
and innovation program ERC AdG CERQUTE (grant agreement No 834266), the State Research
Agency (AEI) TRANQI (PID2019-106888GB-I00/10.13039/501100011033), the Government
of Spain (FIS2020-TRANQI; Severo Ochoa CEX2019-000910-S), Fundació Cellex, Fundació
Mir-Puig, and Generalitat de Catalunya (CERCA, AGAUR). M.M., W.M., N.H.V., and C.F.
acknowledge support from the QuantERA ERA-NET Co-fund (FWF Project I3773-N36) and
the UK Engineering and Physical Sciences Research Council (EPSRC) (EP/P024114/1).
article_number: '50'
article_processing_charge: No
article_type: original
author:
- first_name: Matej
full_name: Pivoluska, Matej
last_name: Pivoluska
- first_name: Martin
full_name: Plesch, Martin
last_name: Plesch
- first_name: Máté
full_name: Farkas, Máté
last_name: Farkas
- first_name: Natalia
full_name: Ruzickova, Natalia
id: D2761128-D73D-11E9-A1BF-BA0DE6697425
last_name: Ruzickova
- first_name: Clara
full_name: Flegel, Clara
last_name: Flegel
- first_name: Natalia Herrera
full_name: Valencia, Natalia Herrera
last_name: Valencia
- first_name: Will
full_name: Mccutcheon, Will
last_name: Mccutcheon
- first_name: Mehul
full_name: Malik, Mehul
last_name: Malik
- first_name: Edgar A.
full_name: Aguilar, Edgar A.
last_name: Aguilar
citation:
ama: Pivoluska M, Plesch M, Farkas M, et al. Semi-device-independent random number
generation with flexible assumptions. npj Quantum Information. 2021;7.
doi:10.1038/s41534-021-00387-1
apa: Pivoluska, M., Plesch, M., Farkas, M., Ruzickova, N., Flegel, C., Valencia,
N. H., … Aguilar, E. A. (2021). Semi-device-independent random number generation
with flexible assumptions. Npj Quantum Information. Springer Nature. https://doi.org/10.1038/s41534-021-00387-1
chicago: Pivoluska, Matej, Martin Plesch, Máté Farkas, Natalia Ruzickova, Clara
Flegel, Natalia Herrera Valencia, Will Mccutcheon, Mehul Malik, and Edgar A. Aguilar.
“Semi-Device-Independent Random Number Generation with Flexible Assumptions.”
Npj Quantum Information. Springer Nature, 2021. https://doi.org/10.1038/s41534-021-00387-1.
ieee: M. Pivoluska et al., “Semi-device-independent random number generation
with flexible assumptions,” npj Quantum Information, vol. 7. Springer Nature,
2021.
ista: Pivoluska M, Plesch M, Farkas M, Ruzickova N, Flegel C, Valencia NH, Mccutcheon
W, Malik M, Aguilar EA. 2021. Semi-device-independent random number generation
with flexible assumptions. npj Quantum Information. 7, 50.
mla: Pivoluska, Matej, et al. “Semi-Device-Independent Random Number Generation
with Flexible Assumptions.” Npj Quantum Information, vol. 7, 50, Springer
Nature, 2021, doi:10.1038/s41534-021-00387-1.
short: M. Pivoluska, M. Plesch, M. Farkas, N. Ruzickova, C. Flegel, N.H. Valencia,
W. Mccutcheon, M. Malik, E.A. Aguilar, Npj Quantum Information 7 (2021).
date_created: 2021-03-21T23:01:19Z
date_published: 2021-03-15T00:00:00Z
date_updated: 2023-08-07T14:17:26Z
day: '15'
ddc:
- '530'
department:
- _id: FyKo
doi: 10.1038/s41534-021-00387-1
external_id:
isi:
- '000629173100001'
file:
- access_level: open_access
checksum: 26d3f2a2c8c8fa8c1002028326b45f64
content_type: application/pdf
creator: dernst
date_created: 2021-03-22T11:09:34Z
date_updated: 2021-03-22T11:09:34Z
file_id: '9274'
file_name: 2021_NPJQuantumInformation_Pivoluska.pdf
file_size: 1360271
relation: main_file
success: 1
file_date_updated: 2021-03-22T11:09:34Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
publication: npj Quantum Information
publication_identifier:
eissn:
- 2056-6387
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Semi-device-independent random number generation with flexible assumptions
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 7
year: '2021'
...
---
_id: '9380'
abstract:
- lang: eng
text: Shigella are pathogens originating within the Escherichia lineage but frequently
classified as a separate genus. Shigella genomes contain numerous insertion sequences
(ISs) that lead to pseudogenisation of affected genes and an increase of non-homologous
recombination. Here, we study 414 genomes of E. coli and Shigella strains to assess
the contribution of genomic rearrangements to Shigella evolution. We found that
Shigella experienced exceptionally high rates of intragenomic rearrangements and
had a decreased rate of homologous recombination compared to pathogenic and non-pathogenic
E. coli. The high rearrangement rate resulted in independent disruption of syntenic
regions and parallel rearrangements in different Shigella lineages. Specifically,
we identified two types of chromosomally encoded E3 ubiquitin-protein ligases
acquired independently by all Shigella strains that also showed a high level of
sequence conservation in the promoter and further in the 5′-intergenic region.
In the only available enteroinvasive E. coli (EIEC) strain, which is a pathogenic
E. coli with a phenotype intermediate between Shigella and non-pathogenic E. coli,
we found a rate of genome rearrangements comparable to those in other E. coli
and no functional copies of the two Shigella-specific E3 ubiquitin ligases. These
data indicate that the accumulation of ISs influenced many aspects of genome evolution
and played an important role in the evolution of intracellular pathogens. Our
research demonstrates the power of comparative genomics-based on synteny block
composition and an important role of non-coding regions in the evolution of genomic
islands.
acknowledgement: We thank Fyodor Kondrashov for valuable advice and manuscript proofreading.
We also thank Alla Mikheenko for assistance with Circos.
article_number: '628622'
article_processing_charge: No
article_type: original
author:
- first_name: Zaira
full_name: Seferbekova, Zaira
last_name: Seferbekova
- first_name: Alexey
full_name: Zabelkin, Alexey
last_name: Zabelkin
- first_name: Yulia
full_name: Yakovleva, Yulia
last_name: Yakovleva
- first_name: Robert
full_name: Afasizhev, Robert
last_name: Afasizhev
- first_name: Natalia O.
full_name: Dranenko, Natalia O.
last_name: Dranenko
- first_name: Nikita
full_name: Alexeev, Nikita
last_name: Alexeev
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
citation:
ama: Seferbekova Z, Zabelkin A, Yakovleva Y, et al. High rates of genome rearrangements
and pathogenicity of Shigella spp. Frontiers in Microbiology. 2021;12.
doi:10.3389/fmicb.2021.628622
apa: Seferbekova, Z., Zabelkin, A., Yakovleva, Y., Afasizhev, R., Dranenko, N. O.,
Alexeev, N., … Bochkareva, O. (2021). High rates of genome rearrangements and
pathogenicity of Shigella spp. Frontiers in Microbiology. Frontiers. https://doi.org/10.3389/fmicb.2021.628622
chicago: Seferbekova, Zaira, Alexey Zabelkin, Yulia Yakovleva, Robert Afasizhev,
Natalia O. Dranenko, Nikita Alexeev, Mikhail S. Gelfand, and Olga Bochkareva.
“High Rates of Genome Rearrangements and Pathogenicity of Shigella Spp.” Frontiers
in Microbiology. Frontiers, 2021. https://doi.org/10.3389/fmicb.2021.628622.
ieee: Z. Seferbekova et al., “High rates of genome rearrangements and pathogenicity
of Shigella spp,” Frontiers in Microbiology, vol. 12. Frontiers, 2021.
ista: Seferbekova Z, Zabelkin A, Yakovleva Y, Afasizhev R, Dranenko NO, Alexeev
N, Gelfand MS, Bochkareva O. 2021. High rates of genome rearrangements and pathogenicity
of Shigella spp. Frontiers in Microbiology. 12, 628622.
mla: Seferbekova, Zaira, et al. “High Rates of Genome Rearrangements and Pathogenicity
of Shigella Spp.” Frontiers in Microbiology, vol. 12, 628622, Frontiers,
2021, doi:10.3389/fmicb.2021.628622.
short: Z. Seferbekova, A. Zabelkin, Y. Yakovleva, R. Afasizhev, N.O. Dranenko, N.
Alexeev, M.S. Gelfand, O. Bochkareva, Frontiers in Microbiology 12 (2021).
date_created: 2021-05-09T22:01:38Z
date_published: 2021-04-12T00:00:00Z
date_updated: 2023-08-08T13:30:39Z
day: '12'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.3389/fmicb.2021.628622
ec_funded: 1
external_id:
isi:
- '000643713300001'
file:
- access_level: open_access
checksum: 2f856543add59273a482a7f326fc0400
content_type: application/pdf
creator: kschuh
date_created: 2021-05-11T13:05:52Z
date_updated: 2021-05-11T13:05:52Z
file_id: '9384'
file_name: 2021_Frontiers_Microbiology_Seferbekova.pdf
file_size: 14362316
relation: main_file
success: 1
file_date_updated: 2021-05-11T13:05:52Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Frontiers in Microbiology
publication_identifier:
eissn:
- 1664-302X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: High rates of genome rearrangements and pathogenicity of Shigella spp
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2021'
...
---
_id: '9910'
abstract:
- lang: eng
text: Adult height inspired the first biometrical and quantitative genetic studies
and is a test-case trait for understanding heritability. The studies of height
led to formulation of the classical polygenic model, that has a profound influence
on the way we view and analyse complex traits. An essential part of the classical
model is an assumption of additivity of effects and normality of the distribution
of the residuals. However, it may be expected that the normal approximation will
become insufficient in bigger studies. Here, we demonstrate that when the height
of hundreds of thousands of individuals is analysed, the model complexity needs
to be increased to include non-additive interactions between sex, environment
and genes. Alternatively, the use of log-normal approximation allowed us to still
use the additive effects model. These findings are important for future genetic
and methodologic studies that make use of adult height as an exemplar trait.
acknowledgement: "We are grateful to Marianna Bevova and Pavel Borodin for fruitful
discussion and help with conceptualising our findings and to Lennart C. Karssen
for help with handling the UK Biobank data.\r\n\r\nFunding\r\nThis research has
been conducted using the UK Biobank Resource (project # 41601, “Non-additive effects
in control of complex human traits”). The work of SAS, IAK, and TIS were supported
by Russian Ministry of Science and Education under the 5–100 Excellence Programme.
The work of YSA and TIA was supported by the Ministry of Education and Science of
the RF via the Institute of Cytology and Genetics SB RAS (project number 0324-2019-0040-C-01/AAAA-A17-117092070032-4).
FAK is supported by the ERC Consolidator Grant (ChrFL: 771209)."
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Sergei A.
full_name: Slavskii, Sergei A.
last_name: Slavskii
- first_name: Ivan A.
full_name: Kuznetsov, Ivan A.
last_name: Kuznetsov
- first_name: Tatiana I.
full_name: Shashkova, Tatiana I.
last_name: Shashkova
- first_name: Georgii A.
full_name: Bazykin, Georgii A.
last_name: Bazykin
- first_name: Tatiana I.
full_name: Axenovich, Tatiana I.
last_name: Axenovich
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Yurii S.
full_name: Aulchenko, Yurii S.
last_name: Aulchenko
citation:
ama: Slavskii SA, Kuznetsov IA, Shashkova TI, et al. The limits of normal approximation
for adult height. European Journal of Human Genetics. 2021;29(7):1082-1091.
doi:10.1038/s41431-021-00836-7
apa: Slavskii, S. A., Kuznetsov, I. A., Shashkova, T. I., Bazykin, G. A., Axenovich,
T. I., Kondrashov, F., & Aulchenko, Y. S. (2021). The limits of normal approximation
for adult height. European Journal of Human Genetics. Springer Nature.
https://doi.org/10.1038/s41431-021-00836-7
chicago: Slavskii, Sergei A., Ivan A. Kuznetsov, Tatiana I. Shashkova, Georgii A.
Bazykin, Tatiana I. Axenovich, Fyodor Kondrashov, and Yurii S. Aulchenko. “The
Limits of Normal Approximation for Adult Height.” European Journal of Human
Genetics. Springer Nature, 2021. https://doi.org/10.1038/s41431-021-00836-7.
ieee: S. A. Slavskii et al., “The limits of normal approximation for adult
height,” European Journal of Human Genetics, vol. 29, no. 7. Springer Nature,
pp. 1082–1091, 2021.
ista: Slavskii SA, Kuznetsov IA, Shashkova TI, Bazykin GA, Axenovich TI, Kondrashov
F, Aulchenko YS. 2021. The limits of normal approximation for adult height. European
Journal of Human Genetics. 29(7), 1082–1091.
mla: Slavskii, Sergei A., et al. “The Limits of Normal Approximation for Adult Height.”
European Journal of Human Genetics, vol. 29, no. 7, Springer Nature, 2021,
pp. 1082–91, doi:10.1038/s41431-021-00836-7.
short: S.A. Slavskii, I.A. Kuznetsov, T.I. Shashkova, G.A. Bazykin, T.I. Axenovich,
F. Kondrashov, Y.S. Aulchenko, European Journal of Human Genetics 29 (2021) 1082–1091.
date_created: 2021-08-15T22:01:28Z
date_published: 2021-07-01T00:00:00Z
date_updated: 2023-08-11T10:33:42Z
day: '01'
ddc:
- '576'
department:
- _id: FyKo
doi: 10.1038/s41431-021-00836-7
ec_funded: 1
external_id:
isi:
- '000625853200001'
pmid:
- '33664501'
file:
- access_level: open_access
checksum: a676d76f91b0dbe0504c63e469129c2a
content_type: application/pdf
creator: asandaue
date_created: 2021-08-16T09:14:36Z
date_updated: 2021-08-16T09:14:36Z
file_id: '9921'
file_name: 2021_EuropeanJournalOfHumanGenetics_Slavskii.pdf
file_size: 1079395
relation: main_file
success: 1
file_date_updated: 2021-08-16T09:14:36Z
has_accepted_license: '1'
intvolume: ' 29'
isi: 1
issue: '7'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 1082-1091
pmid: 1
project:
- _id: 26580278-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '771209'
name: Characterizing the fitness landscape on population and global scales
publication: European Journal of Human Genetics
publication_identifier:
eissn:
- '14765438'
issn:
- '10184813'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: The limits of normal approximation for adult height
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 29
year: '2021'
...
---
_id: '9905'
abstract:
- lang: eng
text: Vaccines are thought to be the best available solution for controlling the
ongoing SARS-CoV-2 pandemic. However, the emergence of vaccine-resistant strains
may come too rapidly for current vaccine developments to alleviate the health,
economic and social consequences of the pandemic. To quantify and characterize
the risk of such a scenario, we created a SIR-derived model with initial stochastic
dynamics of the vaccine-resistant strain to study the probability of its emergence
and establishment. Using parameters realistically resembling SARS-CoV-2 transmission,
we model a wave-like pattern of the pandemic and consider the impact of the rate
of vaccination and the strength of non-pharmaceutical intervention measures on
the probability of emergence of a resistant strain. As expected, we found that
a fast rate of vaccination decreases the probability of emergence of a resistant
strain. Counterintuitively, when a relaxation of non-pharmaceutical interventions
happened at a time when most individuals of the population have already been vaccinated
the probability of emergence of a resistant strain was greatly increased. Consequently,
we show that a period of transmission reduction close to the end of the vaccination
campaign can substantially reduce the probability of resistant strain establishment.
Our results suggest that policymakers and individuals should consider maintaining
non-pharmaceutical interventions and transmission-reducing behaviours throughout
the entire vaccination period.
acknowledgement: We thank Alexey Kondrashov, Nick Machnik, Raimundo Julian Saona Urmeneta,
Gasper Tkacik and Nick Barton for fruitful discussions. We also thank participants
of EvoLunch seminar at IST Austria and the internal seminar at the Banco de España
for useful comments. The opinions expressed in this document are exclusively of
the authors and, therefore, do not necessarily coincide with those of the Banco
de España or the Eurosystem. ETD is supported by the Swiss National Science and
Louis Jeantet Foundation. The work of FAK was in part supported by the ERC Consolidator
Grant (771209-CharFL).
article_number: '15729'
article_processing_charge: Yes
article_type: original
author:
- first_name: Simon
full_name: Rella, Simon
id: B4765ACA-AA38-11E9-AC9A-0930E6697425
last_name: Rella
- first_name: Yuliya A.
full_name: Kulikova, Yuliya A.
last_name: Kulikova
- first_name: Emmanouil T.
full_name: Dermitzakis, Emmanouil T.
last_name: Dermitzakis
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
citation:
ama: Rella S, Kulikova YA, Dermitzakis ET, Kondrashov F. Rates of SARS-CoV-2 transmission
and vaccination impact the fate of vaccine-resistant strains. Scientific Reports.
2021;11(1). doi:10.1038/s41598-021-95025-3
apa: Rella, S., Kulikova, Y. A., Dermitzakis, E. T., & Kondrashov, F. (2021).
Rates of SARS-CoV-2 transmission and vaccination impact the fate of vaccine-resistant
strains. Scientific Reports. Springer Nature. https://doi.org/10.1038/s41598-021-95025-3
chicago: Rella, Simon, Yuliya A. Kulikova, Emmanouil T. Dermitzakis, and Fyodor
Kondrashov. “Rates of SARS-CoV-2 Transmission and Vaccination Impact the Fate
of Vaccine-Resistant Strains.” Scientific Reports. Springer Nature, 2021.
https://doi.org/10.1038/s41598-021-95025-3.
ieee: S. Rella, Y. A. Kulikova, E. T. Dermitzakis, and F. Kondrashov, “Rates of
SARS-CoV-2 transmission and vaccination impact the fate of vaccine-resistant strains,”
Scientific Reports, vol. 11, no. 1. Springer Nature, 2021.
ista: Rella S, Kulikova YA, Dermitzakis ET, Kondrashov F. 2021. Rates of SARS-CoV-2
transmission and vaccination impact the fate of vaccine-resistant strains. Scientific
Reports. 11(1), 15729.
mla: Rella, Simon, et al. “Rates of SARS-CoV-2 Transmission and Vaccination Impact
the Fate of Vaccine-Resistant Strains.” Scientific Reports, vol. 11, no.
1, 15729, Springer Nature, 2021, doi:10.1038/s41598-021-95025-3.
short: S. Rella, Y.A. Kulikova, E.T. Dermitzakis, F. Kondrashov, Scientific Reports
11 (2021).
date_created: 2021-08-15T22:01:26Z
date_published: 2021-07-30T00:00:00Z
date_updated: 2023-08-11T10:42:58Z
day: '30'
ddc:
- '570'
- '610'
department:
- _id: FyKo
doi: 10.1038/s41598-021-95025-3
ec_funded: 1
external_id:
isi:
- '000683329100001'
pmid:
- '34330988'
file:
- access_level: open_access
checksum: ac86892ed17e6724c7251844da5cef5c
content_type: application/pdf
creator: asandaue
date_created: 2021-08-16T11:36:49Z
date_updated: 2021-08-16T11:36:49Z
file_id: '9927'
file_name: 2021_ScientificReports_Rella.pdf
file_size: 3432001
relation: main_file
success: 1
file_date_updated: 2021-08-16T11:36:49Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26580278-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '771209'
name: Characterizing the fitness landscape on population and global scales
publication: Scientific Reports
publication_identifier:
eissn:
- '20452322'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Website
relation: press_release
url: https://ist.ac.at/en/news/counterintuitive-dynamics-threaten-the-end-of-the-pandemic/
scopus_import: '1'
status: public
title: Rates of SARS-CoV-2 transmission and vaccination impact the fate of vaccine-resistant
strains
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2021'
...
---
_id: '7603'
abstract:
- lang: eng
text: Plants are exposed to a variety of abiotic and biotic stresses that may result
in DNA damage. Endogenous processes - such as DNA replication, DNA recombination,
respiration, or photosynthesis - are also a threat to DNA integrity. It is therefore
essential to understand the strategies plants have developed for DNA damage detection,
signaling, and repair. Alternative splicing (AS) is a key post-transcriptional
process with a role in regulation of gene expression. Recent studies demonstrate
that the majority of intron-containing genes in plants are alternatively spliced,
highlighting the importance of AS in plant development and stress response. Not
only does AS ensure a versatile proteome and influence the abundance and availability
of proteins greatly, it has also emerged as an important player in the DNA damage
response (DDR) in animals. Despite extensive studies of DDR carried out in plants,
its regulation at the level of AS has not been comprehensively addressed. Here,
we provide some insights into the interplay between AS and DDR in plants.
article_number: '91'
article_processing_charge: No
article_type: original
author:
- first_name: Barbara Anna
full_name: Nimeth, Barbara Anna
last_name: Nimeth
- first_name: Stefan
full_name: Riegler, Stefan
id: FF6018E0-D806-11E9-8E43-0B14E6697425
last_name: Riegler
orcid: 0000-0003-3413-1343
- first_name: Maria
full_name: Kalyna, Maria
last_name: Kalyna
citation:
ama: Nimeth BA, Riegler S, Kalyna M. Alternative splicing and DNA damage response
in plants. Frontiers in Plant Science. 2020;11. doi:10.3389/fpls.2020.00091
apa: Nimeth, B. A., Riegler, S., & Kalyna, M. (2020). Alternative splicing and
DNA damage response in plants. Frontiers in Plant Science. Frontiers. https://doi.org/10.3389/fpls.2020.00091
chicago: Nimeth, Barbara Anna, Stefan Riegler, and Maria Kalyna. “Alternative Splicing
and DNA Damage Response in Plants.” Frontiers in Plant Science. Frontiers,
2020. https://doi.org/10.3389/fpls.2020.00091.
ieee: B. A. Nimeth, S. Riegler, and M. Kalyna, “Alternative splicing and DNA damage
response in plants,” Frontiers in Plant Science, vol. 11. Frontiers, 2020.
ista: Nimeth BA, Riegler S, Kalyna M. 2020. Alternative splicing and DNA damage
response in plants. Frontiers in Plant Science. 11, 91.
mla: Nimeth, Barbara Anna, et al. “Alternative Splicing and DNA Damage Response
in Plants.” Frontiers in Plant Science, vol. 11, 91, Frontiers, 2020, doi:10.3389/fpls.2020.00091.
short: B.A. Nimeth, S. Riegler, M. Kalyna, Frontiers in Plant Science 11 (2020).
date_created: 2020-03-22T23:00:46Z
date_published: 2020-02-19T00:00:00Z
date_updated: 2023-08-18T07:05:18Z
day: '19'
ddc:
- '580'
department:
- _id: FyKo
doi: 10.3389/fpls.2020.00091
external_id:
isi:
- '000518903600001'
file:
- access_level: open_access
checksum: 57c37209f7b6712ced86c0f11b2be74e
content_type: application/pdf
creator: dernst
date_created: 2020-03-23T09:03:40Z
date_updated: 2020-07-14T12:48:01Z
file_id: '7607'
file_name: 2020_FrontiersPlants_Nimeth.pdf
file_size: 507414
relation: main_file
file_date_updated: 2020-07-14T12:48:01Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: Alternative splicing and DNA damage response in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '7622'
abstract:
- lang: eng
text: The International Young Physicists' Tournament (IYPT) continued in 2018 in
Beijing, China and 2019 in Warsaw, Poland with its 31st and 32nd editions. The
IYPT is a modern scientific competition for teams of high school students, also
known as the Physics World Cup. It involves long-term theoretical and experimental
work focused on solving 17 publicly announced open-ended problems in teams of
five. On top of that, teams have to present their solutions in front of other
teams and a scientific jury, and get opposed and reviewed by their peers. Here
we present a brief information about the competition with a specific focus on
one of the IYPT 2018 tasks, the 'Ring Oiler'. This seemingly simple mechanical
problem appeared to be of such a complexity that even the dozens of participating
teams and jurying scientists were not able to solve all of its subtleties.
article_number: '034001'
article_processing_charge: No
article_type: original
author:
- first_name: Martin
full_name: Plesch, Martin
last_name: Plesch
- first_name: Samuel
full_name: Plesník, Samuel
last_name: Plesník
- first_name: Natalia
full_name: Ruzickova, Natalia
id: D2761128-D73D-11E9-A1BF-BA0DE6697425
last_name: Ruzickova
citation:
ama: Plesch M, Plesník S, Ruzickova N. The IYPT and the “Ring Oiler” problem. European
Journal of Physics. 2020;41(3). doi:10.1088/1361-6404/ab6414
apa: Plesch, M., Plesník, S., & Ruzickova, N. (2020). The IYPT and the “Ring
Oiler” problem. European Journal of Physics. IOP Publishing. https://doi.org/10.1088/1361-6404/ab6414
chicago: Plesch, Martin, Samuel Plesník, and Natalia Ruzickova. “The IYPT and the
‘Ring Oiler’ Problem.” European Journal of Physics. IOP Publishing, 2020.
https://doi.org/10.1088/1361-6404/ab6414.
ieee: M. Plesch, S. Plesník, and N. Ruzickova, “The IYPT and the ‘Ring Oiler’ problem,”
European Journal of Physics, vol. 41, no. 3. IOP Publishing, 2020.
ista: Plesch M, Plesník S, Ruzickova N. 2020. The IYPT and the ‘Ring Oiler’ problem.
European Journal of Physics. 41(3), 034001.
mla: Plesch, Martin, et al. “The IYPT and the ‘Ring Oiler’ Problem.” European
Journal of Physics, vol. 41, no. 3, 034001, IOP Publishing, 2020, doi:10.1088/1361-6404/ab6414.
short: M. Plesch, S. Plesník, N. Ruzickova, European Journal of Physics 41 (2020).
date_created: 2020-03-31T11:25:04Z
date_published: 2020-02-24T00:00:00Z
date_updated: 2023-08-18T10:18:29Z
day: '24'
ddc:
- '530'
department:
- _id: FyKo
doi: 10.1088/1361-6404/ab6414
external_id:
arxiv:
- '1910.03290'
isi:
- '000537425400001'
file:
- access_level: open_access
checksum: 47dda164e33b6c0c6c3ed14aad298376
content_type: application/pdf
creator: dernst
date_created: 2020-04-06T08:53:53Z
date_updated: 2020-07-14T12:48:01Z
file_id: '7641'
file_name: 2020_EuropJourPhysics_Plesch.pdf
file_size: 1533672
relation: main_file
file_date_updated: 2020-07-14T12:48:01Z
has_accepted_license: '1'
intvolume: ' 41'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
publication: European Journal of Physics
publication_identifier:
eissn:
- '13616404'
issn:
- '01430807'
publication_status: published
publisher: IOP Publishing
quality_controlled: '1'
scopus_import: '1'
status: public
title: The IYPT and the 'Ring Oiler' problem
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 41
year: '2020'
...
---
_id: '7931'
abstract:
- lang: eng
text: In the course of sample preparation for Next Generation Sequencing (NGS),
DNA is fragmented by various methods. Fragmentation shows a persistent bias with
regard to the cleavage rates of various dinucleotides. With the exception of CpG
dinucleotides the previously described biases were consistent with results of
the DNA cleavage in solution. Here we computed cleavage rates of all dinucleotides
including the methylated CpG and unmethylated CpG dinucleotides using data of
the Whole Genome Sequencing datasets of the 1000 Genomes project. We found that
the cleavage rate of CpG is significantly higher for the methylated CpG dinucleotides.
Using this information, we developed a classifier for distinguishing cancer and
healthy tissues based on their CpG islands statuses of the fragmentation. A simple
Support Vector Machine classifier based on this algorithm shows an accuracy of
84%. The proposed method allows the detection of epigenetic markers purely based
on mechanochemical DNA fragmentation, which can be detected by a simple analysis
of the NGS sequencing data.
article_number: '8635'
article_processing_charge: No
article_type: original
author:
- first_name: Leonid A.
full_name: Uroshlev, Leonid A.
last_name: Uroshlev
- first_name: Eldar T.
full_name: Abdullaev, Eldar T.
last_name: Abdullaev
- first_name: Iren R.
full_name: Umarova, Iren R.
last_name: Umarova
- first_name: Irina A.
full_name: Il’Icheva, Irina A.
last_name: Il’Icheva
- first_name: Larisa A.
full_name: Panchenko, Larisa A.
last_name: Panchenko
- first_name: Robert V.
full_name: Polozov, Robert V.
last_name: Polozov
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Yury D.
full_name: Nechipurenko, Yury D.
last_name: Nechipurenko
- first_name: Sergei L.
full_name: Grokhovsky, Sergei L.
last_name: Grokhovsky
citation:
ama: Uroshlev LA, Abdullaev ET, Umarova IR, et al. A method for identification of
the methylation level of CpG islands from NGS data. Scientific Reports.
2020;10. doi:10.1038/s41598-020-65406-1
apa: Uroshlev, L. A., Abdullaev, E. T., Umarova, I. R., Il’Icheva, I. A., Panchenko,
L. A., Polozov, R. V., … Grokhovsky, S. L. (2020). A method for identification
of the methylation level of CpG islands from NGS data. Scientific Reports.
Springer Nature. https://doi.org/10.1038/s41598-020-65406-1
chicago: Uroshlev, Leonid A., Eldar T. Abdullaev, Iren R. Umarova, Irina A. Il’Icheva,
Larisa A. Panchenko, Robert V. Polozov, Fyodor Kondrashov, Yury D. Nechipurenko,
and Sergei L. Grokhovsky. “A Method for Identification of the Methylation Level
of CpG Islands from NGS Data.” Scientific Reports. Springer Nature, 2020.
https://doi.org/10.1038/s41598-020-65406-1.
ieee: L. A. Uroshlev et al., “A method for identification of the methylation
level of CpG islands from NGS data,” Scientific Reports, vol. 10. Springer
Nature, 2020.
ista: Uroshlev LA, Abdullaev ET, Umarova IR, Il’Icheva IA, Panchenko LA, Polozov
RV, Kondrashov F, Nechipurenko YD, Grokhovsky SL. 2020. A method for identification
of the methylation level of CpG islands from NGS data. Scientific Reports. 10,
8635.
mla: Uroshlev, Leonid A., et al. “A Method for Identification of the Methylation
Level of CpG Islands from NGS Data.” Scientific Reports, vol. 10, 8635,
Springer Nature, 2020, doi:10.1038/s41598-020-65406-1.
short: L.A. Uroshlev, E.T. Abdullaev, I.R. Umarova, I.A. Il’Icheva, L.A. Panchenko,
R.V. Polozov, F. Kondrashov, Y.D. Nechipurenko, S.L. Grokhovsky, Scientific Reports
10 (2020).
date_created: 2020-06-07T22:00:51Z
date_published: 2020-05-25T00:00:00Z
date_updated: 2023-08-21T07:00:17Z
day: '25'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1038/s41598-020-65406-1
external_id:
isi:
- '000560774200007'
file:
- access_level: open_access
checksum: 099e51611a5b7ca04244d03b2faddf33
content_type: application/pdf
creator: dernst
date_created: 2020-06-08T06:27:32Z
date_updated: 2020-07-14T12:48:05Z
file_id: '7947'
file_name: 2020_ScientificReports_Uroshlev.pdf
file_size: 1001724
relation: main_file
file_date_updated: 2020-07-14T12:48:05Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
publication: Scientific Reports
publication_identifier:
eissn:
- '20452322'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: A method for identification of the methylation level of CpG islands from NGS
data
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2020'
...
---
_id: '8320'
abstract:
- lang: eng
text: The genetic code is considered to use five nucleic bases (adenine, guanine,
cytosine, thymine and uracil), which form two pairs for encoding information in
DNA and two pairs for encoding information in RNA. Nevertheless, in recent years
several artificial base pairs have been developed in attempts to expand the genetic
code. Employment of these additional base pairs increases the information capacity
and variety of DNA sequences, and provides a platform for the site-specific, enzymatic
incorporation of extra functional components into DNA and RNA. As a result, of
the development of such expanded systems, many artificial base pairs have been
synthesized and tested under various conditions. Following many stages of enhancement,
unnatural base pairs have been modified to eliminate their weak points, qualifying
them for specific research needs. Moreover, the first attempts to create a semi-synthetic
organism containing DNA with unnatural base pairs seem to have been successful.
This further extends the possible applications of these kinds of pairs. Herein,
we describe the most significant qualities of unnatural base pairs and their actual
applications.
acknowledgement: We would like to thank our co-workers and members of the Alkalaeva
lab for participating in discussions about the topics covered in this essay.
article_processing_charge: No
article_type: original
author:
- first_name: S. A.
full_name: Mukba, S. A.
last_name: Mukba
- first_name: Petr
full_name: Vlasov, Petr
id: 38BB9AC4-F248-11E8-B48F-1D18A9856A87
last_name: Vlasov
- first_name: P. M.
full_name: Kolosov, P. M.
last_name: Kolosov
- first_name: E. Y.
full_name: Shuvalova, E. Y.
last_name: Shuvalova
- first_name: T. V.
full_name: Egorova, T. V.
last_name: Egorova
- first_name: E. Z.
full_name: Alkalaeva, E. Z.
last_name: Alkalaeva
citation:
ama: 'Mukba SA, Vlasov P, Kolosov PM, Shuvalova EY, Egorova TV, Alkalaeva EZ. Expanding
the genetic code: Unnatural base pairs in biological systems. Molecular Biology.
2020;54(4):475-484. doi:10.1134/S0026893320040111'
apa: 'Mukba, S. A., Vlasov, P., Kolosov, P. M., Shuvalova, E. Y., Egorova, T. V.,
& Alkalaeva, E. Z. (2020). Expanding the genetic code: Unnatural base pairs
in biological systems. Molecular Biology. Springer Nature. https://doi.org/10.1134/S0026893320040111'
chicago: 'Mukba, S. A., Petr Vlasov, P. M. Kolosov, E. Y. Shuvalova, T. V. Egorova,
and E. Z. Alkalaeva. “Expanding the Genetic Code: Unnatural Base Pairs in Biological
Systems.” Molecular Biology. Springer Nature, 2020. https://doi.org/10.1134/S0026893320040111.'
ieee: 'S. A. Mukba, P. Vlasov, P. M. Kolosov, E. Y. Shuvalova, T. V. Egorova, and
E. Z. Alkalaeva, “Expanding the genetic code: Unnatural base pairs in biological
systems,” Molecular Biology, vol. 54, no. 4. Springer Nature, pp. 475–484,
2020.'
ista: 'Mukba SA, Vlasov P, Kolosov PM, Shuvalova EY, Egorova TV, Alkalaeva EZ. 2020.
Expanding the genetic code: Unnatural base pairs in biological systems. Molecular
Biology. 54(4), 475–484.'
mla: 'Mukba, S. A., et al. “Expanding the Genetic Code: Unnatural Base Pairs in
Biological Systems.” Molecular Biology, vol. 54, no. 4, Springer Nature,
2020, pp. 475–84, doi:10.1134/S0026893320040111.'
short: S.A. Mukba, P. Vlasov, P.M. Kolosov, E.Y. Shuvalova, T.V. Egorova, E.Z. Alkalaeva,
Molecular Biology 54 (2020) 475–484.
date_created: 2020-08-30T22:01:11Z
date_published: 2020-08-19T00:00:00Z
date_updated: 2023-08-22T09:01:03Z
day: '19'
department:
- _id: FyKo
doi: 10.1134/S0026893320040111
external_id:
isi:
- '000562110300001'
intvolume: ' 54'
isi: 1
issue: '4'
language:
- iso: eng
month: '08'
oa_version: None
page: 475-484
publication: Molecular Biology
publication_identifier:
eissn:
- '16083245'
issn:
- '00268933'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '8321'
relation: original
status: public
scopus_import: '1'
status: public
title: 'Expanding the genetic code: Unnatural base pairs in biological systems'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 54
year: '2020'
...
---
_id: '8321'
abstract:
- lang: eng
text: The genetic code is considered to use five nucleic bases (adenine, guanine,
cytosine, thymine and uracil), which form two pairs for encoding information in
DNA and two pairs for encoding information in RNA. Nevertheless, in recent years
several artificial base pairs have been developed in attempts to expand the genetic
code. Employment of these additional base pairs increases the information capacity
and variety of DNA sequences, and provides a platform for the site-specific, enzymatic
incorporation of extra functional components into DNA and RNA. As a result, of
the development of such expanded systems, many artificial base pairs have been
synthesized and tested under various conditions. Following many stages of enhancement,
unnatural base pairs have been modified to eliminate their weak points, qualifying
them for specific research needs. Moreover, the first attempts to create a semi-synthetic
organism containing DNA with unnatural base pairs seem to have been successful.
This further extends the possible applications of these kinds of pairs. Herein,
we describe the most significant qualities of unnatural base pairs and their actual
applications.
article_processing_charge: No
article_type: original
author:
- first_name: S. A.
full_name: Mukba, S. A.
last_name: Mukba
- first_name: Petr
full_name: Vlasov, Petr
id: 38BB9AC4-F248-11E8-B48F-1D18A9856A87
last_name: Vlasov
- first_name: P. M.
full_name: Kolosov, P. M.
last_name: Kolosov
- first_name: E. Y.
full_name: Shuvalova, E. Y.
last_name: Shuvalova
- first_name: T. V.
full_name: Egorova, T. V.
last_name: Egorova
- first_name: E. Z.
full_name: Alkalaeva, E. Z.
last_name: Alkalaeva
citation:
ama: 'Mukba SA, Vlasov P, Kolosov PM, Shuvalova EY, Egorova TV, Alkalaeva EZ. Expanding
the genetic code: Unnatural base pairs in biological systems. Molekuliarnaia
biologiia. 2020;54(4):531-541. doi:10.31857/S0026898420040126'
apa: 'Mukba, S. A., Vlasov, P., Kolosov, P. M., Shuvalova, E. Y., Egorova, T. V.,
& Alkalaeva, E. Z. (2020). Expanding the genetic code: Unnatural base pairs
in biological systems. Molekuliarnaia biologiia. Russian Academy of Sciences.
https://doi.org/10.31857/S0026898420040126'
chicago: 'Mukba, S. A., Petr Vlasov, P. M. Kolosov, E. Y. Shuvalova, T. V. Egorova,
and E. Z. Alkalaeva. “Expanding the genetic code: Unnatural base pairs in biological
systems.” Molekuliarnaia biologiia. Russian Academy of Sciences, 2020.
https://doi.org/10.31857/S0026898420040126.'
ieee: 'S. A. Mukba, P. Vlasov, P. M. Kolosov, E. Y. Shuvalova, T. V. Egorova, and
E. Z. Alkalaeva, “Expanding the genetic code: Unnatural base pairs in biological
systems,” Molekuliarnaia biologiia, vol. 54, no. 4. Russian Academy of
Sciences, pp. 531–541, 2020.'
ista: 'Mukba SA, Vlasov P, Kolosov PM, Shuvalova EY, Egorova TV, Alkalaeva EZ. 2020.
Expanding the genetic code: Unnatural base pairs in biological systems. Molekuliarnaia
biologiia. 54(4), 531–541.'
mla: 'Mukba, S. A., et al. “Expanding the genetic code: Unnatural base pairs in
biological systems.” Molekuliarnaia biologiia, vol. 54, no. 4, Russian
Academy of Sciences, 2020, pp. 531–41, doi:10.31857/S0026898420040126.'
short: S.A. Mukba, P. Vlasov, P.M. Kolosov, E.Y. Shuvalova, T.V. Egorova, E.Z. Alkalaeva,
Molekuliarnaia biologiia 54 (2020) 531–541.
date_created: 2020-08-30T22:01:11Z
date_published: 2020-07-01T00:00:00Z
date_updated: 2023-08-22T09:01:02Z
day: '01'
department:
- _id: FyKo
doi: 10.31857/S0026898420040126
external_id:
pmid:
- '32799218'
intvolume: ' 54'
issue: '4'
language:
- iso: rus
month: '07'
oa_version: None
page: 531-541
pmid: 1
publication: Molekuliarnaia biologiia
publication_identifier:
issn:
- '00268984'
publication_status: published
publisher: Russian Academy of Sciences
quality_controlled: '1'
related_material:
record:
- id: '8320'
relation: translation
status: public
scopus_import: '1'
status: public
title: 'Expanding the genetic code: Unnatural base pairs in biological systems'
type: journal_article
user_id: D865714E-FA4E-11E9-B85B-F5C5E5697425
volume: 54
year: '2020'
...
---
_id: '8645'
abstract:
- lang: eng
text: 'Epistasis, the context-dependence of the contribution of an amino acid substitution
to fitness, is common in evolution. To detect epistasis, fitness must be measured
for at least four genotypes: the reference genotype, two different single mutants
and a double mutant with both of the single mutations. For higher-order epistasis
of the order n, fitness has to be measured for all 2n genotypes of an n-dimensional
hypercube in genotype space forming a ‘combinatorially complete dataset’. So far,
only a handful of such datasets have been produced by manual curation. Concurrently,
random mutagenesis experiments have produced measurements of fitness and other
phenotypes in a high-throughput manner, potentially containing a number of combinatorially
complete datasets. We present an effective recursive algorithm for finding all
hypercube structures in random mutagenesis experimental data. To test the algorithm,
we applied it to the data from a recent HIS3 protein dataset and found all 199
847 053 unique combinatorially complete genotype combinations of dimensionality
ranging from 2 to 12. The algorithm may be useful for researchers looking for
higher-order epistasis in their high-throughput experimental data.'
acknowledgement: 'This work was supported by the European Research Council under the
European Union’s Seventh Framework Programme (FP7/2007-2013, ERC grant agreement
335980_EinME) and Startup package to the Ivankov laboratory at Skolkovo Institute
of Science and Technology. The work was started at the School of Molecular and Theoretical
Biology 2017 supported by the Zimin Foundation. N.S.B. was supported by the Woman
Scientists Support Grant in Centre for Genomic Regulation (CRG). '
article_processing_charge: No
article_type: original
author:
- first_name: Laura A
full_name: Esteban, Laura A
last_name: Esteban
- first_name: Lyubov R
full_name: Lonishin, Lyubov R
last_name: Lonishin
- first_name: Daniil M
full_name: Bobrovskiy, Daniil M
last_name: Bobrovskiy
- first_name: Gregory
full_name: Leleytner, Gregory
last_name: Leleytner
- first_name: Natalya S
full_name: Bogatyreva, Natalya S
last_name: Bogatyreva
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: 'Dmitry N '
full_name: 'Ivankov, Dmitry N '
last_name: Ivankov
citation:
ama: 'Esteban LA, Lonishin LR, Bobrovskiy DM, et al. HypercubeME: Two hundred million
combinatorially complete datasets from a single experiment. Bioinformatics.
2020;36(6):1960-1962. doi:10.1093/bioinformatics/btz841'
apa: 'Esteban, L. A., Lonishin, L. R., Bobrovskiy, D. M., Leleytner, G., Bogatyreva,
N. S., Kondrashov, F., & Ivankov, D. N. (2020). HypercubeME: Two hundred million
combinatorially complete datasets from a single experiment. Bioinformatics.
Oxford Academic. https://doi.org/10.1093/bioinformatics/btz841'
chicago: 'Esteban, Laura A, Lyubov R Lonishin, Daniil M Bobrovskiy, Gregory Leleytner,
Natalya S Bogatyreva, Fyodor Kondrashov, and Dmitry N Ivankov. “HypercubeME:
Two Hundred Million Combinatorially Complete Datasets from a Single Experiment.”
Bioinformatics. Oxford Academic, 2020. https://doi.org/10.1093/bioinformatics/btz841.'
ieee: 'L. A. Esteban et al., “HypercubeME: Two hundred million combinatorially
complete datasets from a single experiment,” Bioinformatics, vol. 36, no.
6. Oxford Academic, pp. 1960–1962, 2020.'
ista: 'Esteban LA, Lonishin LR, Bobrovskiy DM, Leleytner G, Bogatyreva NS, Kondrashov
F, Ivankov DN. 2020. HypercubeME: Two hundred million combinatorially complete
datasets from a single experiment. Bioinformatics. 36(6), 1960–1962.'
mla: 'Esteban, Laura A., et al. “HypercubeME: Two Hundred Million Combinatorially
Complete Datasets from a Single Experiment.” Bioinformatics, vol. 36, no.
6, Oxford Academic, 2020, pp. 1960–62, doi:10.1093/bioinformatics/btz841.'
short: L.A. Esteban, L.R. Lonishin, D.M. Bobrovskiy, G. Leleytner, N.S. Bogatyreva,
F. Kondrashov, D.N. Ivankov, Bioinformatics 36 (2020) 1960–1962.
date_created: 2020-10-11T22:01:14Z
date_published: 2020-03-15T00:00:00Z
date_updated: 2023-08-22T09:57:29Z
day: '15'
ddc:
- '000'
- '570'
department:
- _id: FyKo
doi: 10.1093/bioinformatics/btz841
ec_funded: 1
external_id:
isi:
- '000538696800054'
pmid:
- '31742320'
file:
- access_level: open_access
checksum: 21d6f71839deb3b83e4a356193f72767
content_type: application/pdf
creator: dernst
date_created: 2020-10-12T12:02:09Z
date_updated: 2020-10-12T12:02:09Z
file_id: '8649'
file_name: 2020_Bioinformatics_Esteban.pdf
file_size: 308341
relation: main_file
success: 1
file_date_updated: 2020-10-12T12:02:09Z
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intvolume: ' 36'
isi: 1
issue: '6'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '03'
oa: 1
oa_version: Published Version
page: 1960-1962
pmid: 1
project:
- _id: 26120F5C-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '335980'
name: Systematic investigation of epistasis in molecular evolution
publication: Bioinformatics
publication_identifier:
eissn:
- 1460-2059
issn:
- 1367-4803
publication_status: published
publisher: Oxford Academic
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'HypercubeME: Two hundred million combinatorially complete datasets from a
single experiment'
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 36
year: '2020'
...
---
_id: '8707'
abstract:
- lang: eng
text: Dynamic changes in the three-dimensional (3D) organization of chromatin are
associated with central biological processes, such as transcription, replication
and development. Therefore, the comprehensive identification and quantification
of these changes is fundamental to understanding of evolutionary and regulatory
mechanisms. Here, we present Comparison of Hi-C Experiments using Structural Similarity
(CHESS), an algorithm for the comparison of chromatin contact maps and automatic
differential feature extraction. We demonstrate the robustness of CHESS to experimental
variability and showcase its biological applications on (1) interspecies comparisons
of syntenic regions in human and mouse models; (2) intraspecies identification
of conformational changes in Zelda-depleted Drosophila embryos; (3) patient-specific
aberrant chromatin conformation in a diffuse large B-cell lymphoma sample; and
(4) the systematic identification of chromatin contact differences in high-resolution
Capture-C data. In summary, CHESS is a computationally efficient method for the
comparison and classification of changes in chromatin contact data.
acknowledgement: 'Work in the Vaquerizas laboratory is funded by the Max Planck Society,
the Deutsche Forschungsgemeinschaft (DFG) Priority Programme SPP 2202 ‘Spatial Genome
Architecture in Development and Disease’ (project no. 422857230 to J.M.V.), the
DFG Clinical Research Unit CRU326 ‘Male Germ Cells: from Genes to Function’ (project
no. 329621271 to J.M.V.), the European Union’s Horizon 2020 research and innovation
programme under the Marie Skłodowska-Curie grant agreement no. 643062—ZENCODE-ITN
to J.M.V.) and the Medical Research Council in the UK. This research was partially
funded by the European Union’s H2020 Framework Programme through the European Research
Council (grant no. 609989 to M.A.M.-R.). We thank the support of the Spanish Ministerio
de Ciencia, Innovación y Universidades through grant no. BFU2017-85926-P to M.A.M.-R.
The Centre for Genomic Regulation thanks the support of the Ministerio de Ciencia,
Innovación y Universidades to the European Molecular Biology Laboratory partnership,
the ‘Centro de Excelencia Severo Ochoa 2013–2017’, agreement no. SEV-2012-0208,
the CERCA Programme/Generalitat de Catalunya, Spanish Ministerio de Ciencia, Innovación
y Universidades through the Instituto de Salud Carlos III, the Generalitat de Catalunya
through the Departament de Salut and Departament d’Empresa i Coneixement and cofinancing
by the Spanish Ministerio de Ciencia, Innovación y Universidades with funds from
the European Regional Development Fund corresponding to the 2014–2020 Smart Growth
Operating Program. S.G. thanks the support from the Company of Biologists (grant
no. JCSTF181158) and the European Molecular Biology Organization Short-Term Fellowship
programme.'
article_processing_charge: No
article_type: original
author:
- first_name: Silvia
full_name: ' Galan, Silvia'
last_name: ' Galan'
- first_name: Nick N
full_name: Machnik, Nick N
id: 3591A0AA-F248-11E8-B48F-1D18A9856A87
last_name: Machnik
orcid: 0000-0001-6617-9742
- first_name: Kai
full_name: Kruse, Kai
last_name: Kruse
- first_name: Noelia
full_name: Díaz, Noelia
last_name: Díaz
- first_name: Marc A
full_name: Marti-Renom, Marc A
last_name: Marti-Renom
- first_name: Juan M
full_name: Vaquerizas, Juan M
last_name: Vaquerizas
citation:
ama: Galan S, Machnik NN, Kruse K, Díaz N, Marti-Renom MA, Vaquerizas JM. CHESS
enables quantitative comparison of chromatin contact data and automatic feature
extraction. Nature Genetics. 2020;52:1247-1255. doi:10.1038/s41588-020-00712-y
apa: Galan, S., Machnik, N. N., Kruse, K., Díaz, N., Marti-Renom, M. A., & Vaquerizas,
J. M. (2020). CHESS enables quantitative comparison of chromatin contact data
and automatic feature extraction. Nature Genetics. Springer Nature. https://doi.org/10.1038/s41588-020-00712-y
chicago: Galan, Silvia, Nick N Machnik, Kai Kruse, Noelia Díaz, Marc A Marti-Renom,
and Juan M Vaquerizas. “CHESS Enables Quantitative Comparison of Chromatin Contact
Data and Automatic Feature Extraction.” Nature Genetics. Springer Nature,
2020. https://doi.org/10.1038/s41588-020-00712-y.
ieee: S. Galan, N. N. Machnik, K. Kruse, N. Díaz, M. A. Marti-Renom, and J. M.
Vaquerizas, “CHESS enables quantitative comparison of chromatin contact data and
automatic feature extraction,” Nature Genetics, vol. 52. Springer Nature,
pp. 1247–1255, 2020.
ista: Galan S, Machnik NN, Kruse K, Díaz N, Marti-Renom MA, Vaquerizas JM. 2020.
CHESS enables quantitative comparison of chromatin contact data and automatic
feature extraction. Nature Genetics. 52, 1247–1255.
mla: Galan, Silvia, et al. “CHESS Enables Quantitative Comparison of Chromatin Contact
Data and Automatic Feature Extraction.” Nature Genetics, vol. 52, Springer
Nature, 2020, pp. 1247–55, doi:10.1038/s41588-020-00712-y.
short: S. Galan, N.N. Machnik, K. Kruse, N. Díaz, M.A. Marti-Renom, J.M. Vaquerizas,
Nature Genetics 52 (2020) 1247–1255.
date_created: 2020-10-25T23:01:20Z
date_published: 2020-10-19T00:00:00Z
date_updated: 2023-08-22T10:37:10Z
day: '19'
department:
- _id: FyKo
doi: 10.1038/s41588-020-00712-y
external_id:
isi:
- '000579693500004'
pmid:
- '33077914'
intvolume: ' 52'
isi: 1
language:
- iso: eng
month: '10'
oa_version: None
page: 1247-1255
pmid: 1
publication: Nature Genetics
publication_identifier:
eissn:
- '15461718'
issn:
- '10614036'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: CHESS enables quantitative comparison of chromatin contact data and automatic
feature extraction
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 52
year: '2020'
...
---
_id: '8700'
abstract:
- lang: eng
text: Translation termination is a finishing step of protein biosynthesis. The significant
role in this process belongs not only to protein factors of translation termination
but also to the nearest nucleotide environment of stop codons. There are numerous
descriptions of stop codons readthrough, which is due to specific nucleotide sequences
behind them. However, represented data are segmental and don’t explain the mechanism
of the nucleotide context influence on translation termination. It is well known
that stop codon UAA usage is preferential for A/T-rich genes, and UAG, UGA—for
G/C-rich genes, which is related to an expression level of these genes. We investigated
the connection between a frequency of nucleotides occurrence in 3' area of stop
codons in the human genome and their influence on translation termination efficiency.
We found that 3' context motif, which is cognate to the sequence of a stop codon,
stimulates translation termination. At the same time, the nucleotide composition
of 3' sequence that differs from stop codon, decreases translation termination
efficiency.
acknowledgement: We would like to thank the staff of CCU Genome for sequencing, Tat’yana
Pestova, Christopher Helen, and Lyudmila Yur’evna Frolova for the plasmids provided,
as well as the laboratory staff for productive discussion of the results. We also
thank former laboratory employees Yuliya Vladimirovna Bocharova and Polina Nikolaevna
Kryuchkova for the exceptional contribution to the present work.
article_processing_charge: No
article_type: original
author:
- first_name: E. E.
full_name: Sokolova, E. E.
last_name: Sokolova
- first_name: Petr
full_name: Vlasov, Petr
id: 38BB9AC4-F248-11E8-B48F-1D18A9856A87
last_name: Vlasov
- first_name: T. V.
full_name: Egorova, T. V.
last_name: Egorova
- first_name: A. V.
full_name: Shuvalov, A. V.
last_name: Shuvalov
- first_name: E. Z.
full_name: Alkalaeva, E. Z.
last_name: Alkalaeva
citation:
ama: Sokolova EE, Vlasov P, Egorova TV, Shuvalov AV, Alkalaeva EZ. The influence
of A/G composition of 3’ stop codon contexts on translation termination efficiency
in eukaryotes. Molecular Biology. 2020;54(5):739-748. doi:10.1134/S0026893320050088
apa: Sokolova, E. E., Vlasov, P., Egorova, T. V., Shuvalov, A. V., & Alkalaeva,
E. Z. (2020). The influence of A/G composition of 3’ stop codon contexts on translation
termination efficiency in eukaryotes. Molecular Biology. Springer Nature.
https://doi.org/10.1134/S0026893320050088
chicago: Sokolova, E. E., Petr Vlasov, T. V. Egorova, A. V. Shuvalov, and E. Z.
Alkalaeva. “The Influence of A/G Composition of 3’ Stop Codon Contexts on Translation
Termination Efficiency in Eukaryotes.” Molecular Biology. Springer Nature,
2020. https://doi.org/10.1134/S0026893320050088.
ieee: E. E. Sokolova, P. Vlasov, T. V. Egorova, A. V. Shuvalov, and E. Z. Alkalaeva,
“The influence of A/G composition of 3’ stop codon contexts on translation termination
efficiency in eukaryotes,” Molecular Biology, vol. 54, no. 5. Springer
Nature, pp. 739–748, 2020.
ista: Sokolova EE, Vlasov P, Egorova TV, Shuvalov AV, Alkalaeva EZ. 2020. The influence
of A/G composition of 3’ stop codon contexts on translation termination efficiency
in eukaryotes. Molecular Biology. 54(5), 739–748.
mla: Sokolova, E. E., et al. “The Influence of A/G Composition of 3’ Stop Codon
Contexts on Translation Termination Efficiency in Eukaryotes.” Molecular Biology,
vol. 54, no. 5, Springer Nature, 2020, pp. 739–48, doi:10.1134/S0026893320050088.
short: E.E. Sokolova, P. Vlasov, T.V. Egorova, A.V. Shuvalov, E.Z. Alkalaeva, Molecular
Biology 54 (2020) 739–748.
date_created: 2020-10-25T23:01:17Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2023-08-22T10:39:38Z
day: '01'
department:
- _id: FyKo
doi: 10.1134/S0026893320050088
external_id:
isi:
- '000579441200009'
intvolume: ' 54'
isi: 1
issue: '5'
language:
- iso: eng
month: '09'
oa_version: None
page: 739-748
publication: Molecular Biology
publication_identifier:
eissn:
- '16083245'
issn:
- '00268933'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '8701'
relation: original
status: public
scopus_import: '1'
status: public
title: The influence of A/G composition of 3' stop codon contexts on translation termination
efficiency in eukaryotes
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 54
year: '2020'
...
---
_id: '8701'
abstract:
- lang: eng
text: Translation termination is a finishing step of protein biosynthesis. The significant
role in this process belongs not only to protein factors of translation termination
but also to the nearest nucleotide environment of stop codons. There are numerous
descriptions of stop codons readthrough, which is due to specific nucleotide sequences
behind them. However, represented data are segmental and don’t explain the mechanism
of the nucleotide context influence on translation termination. It is well known
that stop codon UAA usage is preferential for A/T-rich genes, and UAG, UGA—for
G/C-rich genes, which is related to an expression level of these genes. We investigated
the connection between a frequency of nucleotides occurrence in 3' area of stop
codons in the human genome and their influence on translation termination efficiency.
We found that 3' context motif, which is cognate to the sequence of a stop codon,
stimulates translation termination. At the same time, the nucleotide composition
of 3' sequence that differs from stop codon, decreases translation termination
efficiency.
article_processing_charge: No
article_type: original
author:
- first_name: E. E.
full_name: Sokolova, E. E.
last_name: Sokolova
- first_name: Petr
full_name: Vlasov, Petr
id: 38BB9AC4-F248-11E8-B48F-1D18A9856A87
last_name: Vlasov
- first_name: T. V.
full_name: Egorova, T. V.
last_name: Egorova
- first_name: A. V.
full_name: Shuvalov, A. V.
last_name: Shuvalov
- first_name: E. Z.
full_name: Alkalaeva, E. Z.
last_name: Alkalaeva
citation:
ama: Sokolova EE, Vlasov P, Egorova TV, Shuvalov AV, Alkalaeva EZ. The influence
of A/G composition of 3’ stop codon contexts on translation termination efficiency
in eukaryotes. Molekuliarnaia biologiia. 2020;54(5):837-848. doi:10.31857/S0026898420050080
apa: Sokolova, E. E., Vlasov, P., Egorova, T. V., Shuvalov, A. V., & Alkalaeva,
E. Z. (2020). The influence of A/G composition of 3’ stop codon contexts on translation
termination efficiency in eukaryotes. Molekuliarnaia biologiia. Russian
Academy of Sciences. https://doi.org/10.31857/S0026898420050080
chicago: Sokolova, E. E., Petr Vlasov, T. V. Egorova, A. V. Shuvalov, and E. Z.
Alkalaeva. “The influence of A/G composition of 3’ stop codon contexts on translation
termination efficiency in eukaryotes.” Molekuliarnaia biologiia. Russian
Academy of Sciences, 2020. https://doi.org/10.31857/S0026898420050080.
ieee: E. E. Sokolova, P. Vlasov, T. V. Egorova, A. V. Shuvalov, and E. Z. Alkalaeva,
“The influence of A/G composition of 3’ stop codon contexts on translation termination
efficiency in eukaryotes,” Molekuliarnaia biologiia, vol. 54, no. 5. Russian
Academy of Sciences, pp. 837–848, 2020.
ista: Sokolova EE, Vlasov P, Egorova TV, Shuvalov AV, Alkalaeva EZ. 2020. The influence
of A/G composition of 3’ stop codon contexts on translation termination efficiency
in eukaryotes. Molekuliarnaia biologiia. 54(5), 837–848.
mla: Sokolova, E. E., et al. “The influence of A/G composition of 3’ stop codon
contexts on translation termination efficiency in eukaryotes.” Molekuliarnaia
biologiia, vol. 54, no. 5, Russian Academy of Sciences, 2020, pp. 837–48,
doi:10.31857/S0026898420050080.
short: E.E. Sokolova, P. Vlasov, T.V. Egorova, A.V. Shuvalov, E.Z. Alkalaeva, Molekuliarnaia
biologiia 54 (2020) 837–848.
date_created: 2020-10-25T23:01:17Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2023-08-22T10:39:37Z
day: '01'
department:
- _id: FyKo
doi: 10.31857/S0026898420050080
external_id:
pmid:
- '33009793'
intvolume: ' 54'
issue: '5'
language:
- iso: rus
month: '09'
oa_version: None
page: 837-848
pmid: 1
publication: Molekuliarnaia biologiia
publication_identifier:
issn:
- '00268984'
publication_status: published
publisher: Russian Academy of Sciences
quality_controlled: '1'
related_material:
record:
- id: '8700'
relation: translation
status: public
scopus_import: '1'
status: public
title: The influence of A/G composition of 3' stop codon contexts on translation termination
efficiency in eukaryotes
type: journal_article
user_id: D865714E-FA4E-11E9-B85B-F5C5E5697425
volume: 54
year: '2020'
...
---
_id: '15071'
abstract:
- lang: eng
text: "A mesophilic methanogenic culture, designated JL01, was isolated from Holocene
permafrost in the Russian Arctic [1]. After long-term extensive cultivation at
15°C it turned out to be a tied binary culture of archaeal (JL01) and bacterial
(Sphaerochaeta associata GLS2) strains.\r\nStrain JL01 was a strict anaerobe and
grew on methanol, acetate and methylamines as energy and carbon sources. Cells
were irregular coccoid, non-motile, non-spore-forming, and Gram-stainpositive.
Optimum conditions for growth were 24-28 oC, pH 6.8–7.3 and 0.075-0.1 M NaCl.\r\nPhylogenetic
tree reconstructions based on 16S rRNA and concatenated alignment of broadly\r\nconserved
protein-coding genes revealed its close relation to Methanosarcina mazei S-6\r\nT
(similarity 99.5%). The comparison of whole genomic sequences (ANI) of the isolate
and the type strain of M.mazei was 98.5%, which is higher than the values recommended
for new species. Thus strain JL01 (=VKM B-2370=JCM 31898) represents the first
M. mazei isolated from permanently subzero Arcticsediments. The long-term co-cultivation
of JL01 with S. associata GLS2T showed the methane production without any additional
carbon and energy sources. Genome analysis of S. associata GLS2T revealed putative
genes involved in methanochondroithin catabolism."
acknowledgement: "The work was supported by of Russian Foundation of Basic Research:
grant № 19-04-00831 for Viktoria Shcherbakova and Olga Troshina, grant № 18-34-00334
for Viktoriia Oshurkova and Vladimir Trubitsyn. \r\nWe thank Dr Natalia Suzina (IBPM
RAS, Federal Research Center Pushchino Center for\r\nBiological Research RAS) for
the help with the microscopic studies, respectively; Dr. Margarita Meyer (Division
of Genetics, Department of Medicine, BWH and HMS, USA) and Dr Fedor Kondrashov (IST,
Austria) for their help in obtaining the genomic sequence of strain JL01. "
article_processing_charge: Yes
author:
- first_name: Viktoriia
full_name: Oshurkova, Viktoriia
last_name: Oshurkova
- first_name: Olga
full_name: Troshina, Olga
last_name: Troshina
- first_name: Vladimir
full_name: Trubitsyn, Vladimir
last_name: Trubitsyn
- first_name: Yana
full_name: Ryzhmanova, Yana
last_name: Ryzhmanova
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Viktoria
full_name: Shcherbakova, Viktoria
last_name: Shcherbakova
citation:
ama: 'Oshurkova V, Troshina O, Trubitsyn V, Ryzhmanova Y, Bochkareva O, Shcherbakova
V. Characterization of methanosarcina mazei JL01 isolated from holocene arctic
permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta
associata GLS2T. In: Proceedings of 1st International Electronic Conference
on Microbiology. MDPI; 2020. doi:10.3390/ecm2020-07116'
apa: 'Oshurkova, V., Troshina, O., Trubitsyn, V., Ryzhmanova, Y., Bochkareva, O.,
& Shcherbakova, V. (2020). Characterization of methanosarcina mazei JL01 isolated
from holocene arctic permafrost and study of the archaeon cooperation with bacterium
Sphaerochaeta associata GLS2T. In Proceedings of 1st International Electronic
Conference on Microbiology. Virtual: MDPI. https://doi.org/10.3390/ecm2020-07116'
chicago: Oshurkova, Viktoriia, Olga Troshina, Vladimir Trubitsyn, Yana Ryzhmanova,
Olga Bochkareva, and Viktoria Shcherbakova. “Characterization of Methanosarcina
Mazei JL01 Isolated from Holocene Arctic Permafrost and Study of the Archaeon
Cooperation with Bacterium Sphaerochaeta Associata GLS2T.” In Proceedings of
1st International Electronic Conference on Microbiology. MDPI, 2020. https://doi.org/10.3390/ecm2020-07116.
ieee: V. Oshurkova, O. Troshina, V. Trubitsyn, Y. Ryzhmanova, O. Bochkareva, and
V. Shcherbakova, “Characterization of methanosarcina mazei JL01 isolated from
holocene arctic permafrost and study of the archaeon cooperation with bacterium
Sphaerochaeta associata GLS2T,” in Proceedings of 1st International Electronic
Conference on Microbiology, Virtual, 2020.
ista: 'Oshurkova V, Troshina O, Trubitsyn V, Ryzhmanova Y, Bochkareva O, Shcherbakova
V. 2020. Characterization of methanosarcina mazei JL01 isolated from holocene
arctic permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta
associata GLS2T. Proceedings of 1st International Electronic Conference on Microbiology.
ECM: Electronic Conference on Microbiology.'
mla: Oshurkova, Viktoriia, et al. “Characterization of Methanosarcina Mazei JL01
Isolated from Holocene Arctic Permafrost and Study of the Archaeon Cooperation
with Bacterium Sphaerochaeta Associata GLS2T.” Proceedings of 1st International
Electronic Conference on Microbiology, MDPI, 2020, doi:10.3390/ecm2020-07116.
short: V. Oshurkova, O. Troshina, V. Trubitsyn, Y. Ryzhmanova, O. Bochkareva, V.
Shcherbakova, in:, Proceedings of 1st International Electronic Conference on Microbiology,
MDPI, 2020.
conference:
end_date: 2020-11-30
location: Virtual
name: 'ECM: Electronic Conference on Microbiology'
start_date: 2020-11-02
date_created: 2024-03-04T11:41:31Z
date_published: 2020-11-02T00:00:00Z
date_updated: 2024-03-20T08:06:22Z
day: '02'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.3390/ecm2020-07116
file:
- access_level: open_access
checksum: d1914af7811a21a4b2744eb51b5834e3
content_type: application/pdf
creator: dernst
date_created: 2024-03-20T08:05:46Z
date_updated: 2024-03-20T08:05:46Z
file_id: '15127'
file_name: 2020_ECM_Oshurkova.pdf
file_size: 595543
relation: main_file
success: 1
file_date_updated: 2024-03-20T08:05:46Z
has_accepted_license: '1'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Proceedings of 1st International Electronic Conference on Microbiology
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Characterization of methanosarcina mazei JL01 isolated from holocene arctic
permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta associata
GLS2T
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '7889'
abstract:
- lang: eng
text: Autoluminescent plants engineered to express a bacterial bioluminescence gene
cluster in plastids have not been widely adopted because of low light output.
We engineered tobacco plants with a fungal bioluminescence system that converts
caffeic acid (present in all plants) into luciferin and report self-sustained
luminescence that is visible to the naked eye. Our findings could underpin development
of a suite of imaging tools for plants.
acknowledgement: "This study was designed, performed and funded by Planta LLC. We
thank K. Wood for assisting in manuscript development. Planta acknowledges support
from the Skolkovo Innovation Centre. We thank D. Bolotin and the Milaboratory (milaboratory.com)
for access to computing and storage infrastructure. We thank S. Shakhov for providing\r\nphotography
equipment. The Synthetic Biology Group is funded by the MRC London Institute of
Medical Sciences (UKRI MC-A658-5QEA0, K.S.S.). K.S.S. is supported by an Imperial
College Research Fellowship. Experiments were partially carried out using equipment
provided by the Institute of Bioorganic Chemistry of the Russian Academy\r\nof Sciences
Сore Facility (CKP IBCH; supported by the Russian Ministry of Education and Science
Grant RFMEFI62117X0018). The F.A.K. lab is supported by ERC grant agreement 771209—CharFL.
This project received funding from the European Union’s Horizon 2020 Research and
Innovation Programme under Marie Skłodowska-Curie\r\nGrant Agreement 665385. K.S.S.
acknowledges support by President’s Grant 075-15-2019-411. Design and assembly of
some of the plasmids was supported by Russian Science Foundation grant 19-74-10102.
Imaging experiments were partially supported by Russian Science Foundation grant
17-14-01169p. LC-MS/MS analyses of extracts were\r\nsupported by Russian Science
Foundation grant 16-14-00052p. Design and assembly of plasmids was partially supported
by grant 075-15-2019-1789 from the Ministry of Science and Higher Education of the
Russian Federation allocated to the Center for Precision Genome Editing and Genetic
Technologies for Biomedicine. The authors\r\nwould like to acknowledge the work
of Genomics Core Facility of the Skolkovo Institute of Science and Technology, which
performed the sequencing and bioinformatic analysis."
article_processing_charge: No
article_type: original
author:
- first_name: Tatiana
full_name: Mitiouchkina, Tatiana
last_name: Mitiouchkina
- first_name: Alexander S.
full_name: Mishin, Alexander S.
last_name: Mishin
- first_name: Louisa
full_name: Gonzalez Somermeyer, Louisa
id: 4720D23C-F248-11E8-B48F-1D18A9856A87
last_name: Gonzalez Somermeyer
orcid: 0000-0001-9139-5383
- first_name: Nadezhda M.
full_name: Markina, Nadezhda M.
last_name: Markina
- first_name: Tatiana V.
full_name: Chepurnyh, Tatiana V.
last_name: Chepurnyh
- first_name: Elena B.
full_name: Guglya, Elena B.
last_name: Guglya
- first_name: Tatiana A.
full_name: Karataeva, Tatiana A.
last_name: Karataeva
- first_name: Kseniia A.
full_name: Palkina, Kseniia A.
last_name: Palkina
- first_name: Ekaterina S.
full_name: Shakhova, Ekaterina S.
last_name: Shakhova
- first_name: Liliia I.
full_name: Fakhranurova, Liliia I.
last_name: Fakhranurova
- first_name: Sofia V.
full_name: Chekova, Sofia V.
last_name: Chekova
- first_name: Aleksandra S.
full_name: Tsarkova, Aleksandra S.
last_name: Tsarkova
- first_name: Yaroslav V.
full_name: Golubev, Yaroslav V.
last_name: Golubev
- first_name: Vadim V.
full_name: Negrebetsky, Vadim V.
last_name: Negrebetsky
- first_name: Sergey A.
full_name: Dolgushin, Sergey A.
last_name: Dolgushin
- first_name: Pavel V.
full_name: Shalaev, Pavel V.
last_name: Shalaev
- first_name: Dmitry
full_name: Shlykov, Dmitry
last_name: Shlykov
- first_name: Olesya A.
full_name: Melnik, Olesya A.
last_name: Melnik
- first_name: Victoria O.
full_name: Shipunova, Victoria O.
last_name: Shipunova
- first_name: Sergey M.
full_name: Deyev, Sergey M.
last_name: Deyev
- first_name: Andrey I.
full_name: Bubyrev, Andrey I.
last_name: Bubyrev
- first_name: Alexander S.
full_name: Pushin, Alexander S.
last_name: Pushin
- first_name: Vladimir V.
full_name: Choob, Vladimir V.
last_name: Choob
- first_name: Sergey V.
full_name: Dolgov, Sergey V.
last_name: Dolgov
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Ilia V.
full_name: Yampolsky, Ilia V.
last_name: Yampolsky
- first_name: Karen S.
full_name: Sarkisyan, Karen S.
last_name: Sarkisyan
citation:
ama: Mitiouchkina T, Mishin AS, Gonzalez Somermeyer L, et al. Plants with genetically
encoded autoluminescence. Nature Biotechnology. 2020;38:944-946. doi:10.1038/s41587-020-0500-9
apa: Mitiouchkina, T., Mishin, A. S., Gonzalez Somermeyer, L., Markina, N. M., Chepurnyh,
T. V., Guglya, E. B., … Sarkisyan, K. S. (2020). Plants with genetically encoded
autoluminescence. Nature Biotechnology. Springer Nature. https://doi.org/10.1038/s41587-020-0500-9
chicago: Mitiouchkina, Tatiana, Alexander S. Mishin, Louisa Gonzalez Somermeyer,
Nadezhda M. Markina, Tatiana V. Chepurnyh, Elena B. Guglya, Tatiana A. Karataeva,
et al. “Plants with Genetically Encoded Autoluminescence.” Nature Biotechnology.
Springer Nature, 2020. https://doi.org/10.1038/s41587-020-0500-9.
ieee: T. Mitiouchkina et al., “Plants with genetically encoded autoluminescence,”
Nature Biotechnology, vol. 38. Springer Nature, pp. 944–946, 2020.
ista: Mitiouchkina T, Mishin AS, Gonzalez Somermeyer L, Markina NM, Chepurnyh TV,
Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, Chekova SV,
Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, Shalaev PV, Shlykov D,
Melnik OA, Shipunova VO, Deyev SM, Bubyrev AI, Pushin AS, Choob VV, Dolgov SV,
Kondrashov F, Yampolsky IV, Sarkisyan KS. 2020. Plants with genetically encoded
autoluminescence. Nature Biotechnology. 38, 944–946.
mla: Mitiouchkina, Tatiana, et al. “Plants with Genetically Encoded Autoluminescence.”
Nature Biotechnology, vol. 38, Springer Nature, 2020, pp. 944–46, doi:10.1038/s41587-020-0500-9.
short: T. Mitiouchkina, A.S. Mishin, L. Gonzalez Somermeyer, N.M. Markina, T.V.
Chepurnyh, E.B. Guglya, T.A. Karataeva, K.A. Palkina, E.S. Shakhova, L.I. Fakhranurova,
S.V. Chekova, A.S. Tsarkova, Y.V. Golubev, V.V. Negrebetsky, S.A. Dolgushin, P.V.
Shalaev, D. Shlykov, O.A. Melnik, V.O. Shipunova, S.M. Deyev, A.I. Bubyrev, A.S.
Pushin, V.V. Choob, S.V. Dolgov, F. Kondrashov, I.V. Yampolsky, K.S. Sarkisyan,
Nature Biotechnology 38 (2020) 944–946.
date_created: 2020-05-25T15:02:00Z
date_published: 2020-04-27T00:00:00Z
date_updated: 2023-09-05T15:30:34Z
day: '27'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1038/s41587-020-0500-9
ec_funded: 1
external_id:
isi:
- '000529298800003'
pmid:
- '32341562'
file:
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checksum: 1b30467500ec6277229a875b06e196d0
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creator: dernst
date_created: 2020-08-28T08:57:07Z
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oa_version: Submitted Version
page: 944-946
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call_identifier: H2020
grant_number: '771209'
name: Characterizing the fitness landscape on population and global scales
publication: Nature Biotechnology
publication_identifier:
eissn:
- 1546-1696
issn:
- 1087-0156
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41587-020-0578-0
scopus_import: '1'
status: public
title: Plants with genetically encoded autoluminescence
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 38
year: '2020'
...
---
_id: '6419'
abstract:
- lang: eng
text: Characterizing the fitness landscape, a representation of fitness for a large
set of genotypes, is key to understanding how genetic information is interpreted
to create functional organisms. Here we determined the evolutionarily-relevant
segment of the fitness landscape of His3, a gene coding for an enzyme in the histidine
synthesis pathway, focusing on combinations of amino acid states found at orthologous
sites of extant species. Just 15% of amino acids found in yeast His3 orthologues
were always neutral while the impact on fitness of the remaining 85% depended
on the genetic background. Furthermore, at 67% of sites, amino acid replacements
were under sign epistasis, having both strongly positive and negative effect in
different genetic backgrounds. 46% of sites were under reciprocal sign epistasis.
The fitness impact of amino acid replacements was influenced by only a few genetic
backgrounds but involved interaction of multiple sites, shaping a rugged fitness
landscape in which many of the shortest paths between highly fit genotypes are
inaccessible.
article_number: e1008079
article_processing_charge: No
author:
- first_name: Victoria
full_name: Pokusaeva, Victoria
id: 3184041C-F248-11E8-B48F-1D18A9856A87
last_name: Pokusaeva
orcid: 0000-0001-7660-444X
- first_name: Dinara R.
full_name: Usmanova, Dinara R.
last_name: Usmanova
- first_name: Ekaterina V.
full_name: Putintseva, Ekaterina V.
last_name: Putintseva
- first_name: Lorena
full_name: Espinar, Lorena
last_name: Espinar
- first_name: Karen
full_name: Sarkisyan, Karen
id: 39A7BF80-F248-11E8-B48F-1D18A9856A87
last_name: Sarkisyan
orcid: 0000-0002-5375-6341
- first_name: Alexander S.
full_name: Mishin, Alexander S.
last_name: Mishin
- first_name: Natalya S.
full_name: Bogatyreva, Natalya S.
last_name: Bogatyreva
- first_name: Dmitry
full_name: Ivankov, Dmitry
id: 49FF1036-F248-11E8-B48F-1D18A9856A87
last_name: Ivankov
- first_name: Arseniy
full_name: Akopyan, Arseniy
id: 430D2C90-F248-11E8-B48F-1D18A9856A87
last_name: Akopyan
orcid: 0000-0002-2548-617X
- first_name: Sergey
full_name: Avvakumov, Sergey
id: 3827DAC8-F248-11E8-B48F-1D18A9856A87
last_name: Avvakumov
- first_name: Inna S.
full_name: Povolotskaya, Inna S.
last_name: Povolotskaya
- first_name: Guillaume J.
full_name: Filion, Guillaume J.
last_name: Filion
- first_name: Lucas B.
full_name: Carey, Lucas B.
last_name: Carey
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
citation:
ama: Pokusaeva V, Usmanova DR, Putintseva EV, et al. An experimental assay of the
interactions of amino acids from orthologous sequences shaping a complex fitness
landscape. PLoS Genetics. 2019;15(4). doi:10.1371/journal.pgen.1008079
apa: Pokusaeva, V., Usmanova, D. R., Putintseva, E. V., Espinar, L., Sarkisyan,
K., Mishin, A. S., … Kondrashov, F. (2019). An experimental assay of the interactions
of amino acids from orthologous sequences shaping a complex fitness landscape.
PLoS Genetics. Public Library of Science. https://doi.org/10.1371/journal.pgen.1008079
chicago: Pokusaeva, Victoria, Dinara R. Usmanova, Ekaterina V. Putintseva, Lorena
Espinar, Karen Sarkisyan, Alexander S. Mishin, Natalya S. Bogatyreva, et al. “An
Experimental Assay of the Interactions of Amino Acids from Orthologous Sequences
Shaping a Complex Fitness Landscape.” PLoS Genetics. Public Library of
Science, 2019. https://doi.org/10.1371/journal.pgen.1008079.
ieee: V. Pokusaeva et al., “An experimental assay of the interactions of
amino acids from orthologous sequences shaping a complex fitness landscape,” PLoS
Genetics, vol. 15, no. 4. Public Library of Science, 2019.
ista: Pokusaeva V, Usmanova DR, Putintseva EV, Espinar L, Sarkisyan K, Mishin AS,
Bogatyreva NS, Ivankov D, Akopyan A, Avvakumov S, Povolotskaya IS, Filion GJ,
Carey LB, Kondrashov F. 2019. An experimental assay of the interactions of amino
acids from orthologous sequences shaping a complex fitness landscape. PLoS Genetics.
15(4), e1008079.
mla: Pokusaeva, Victoria, et al. “An Experimental Assay of the Interactions of Amino
Acids from Orthologous Sequences Shaping a Complex Fitness Landscape.” PLoS
Genetics, vol. 15, no. 4, e1008079, Public Library of Science, 2019, doi:10.1371/journal.pgen.1008079.
short: V. Pokusaeva, D.R. Usmanova, E.V. Putintseva, L. Espinar, K. Sarkisyan, A.S.
Mishin, N.S. Bogatyreva, D. Ivankov, A. Akopyan, S. Avvakumov, I.S. Povolotskaya,
G.J. Filion, L.B. Carey, F. Kondrashov, PLoS Genetics 15 (2019).
date_created: 2019-05-13T07:58:38Z
date_published: 2019-04-10T00:00:00Z
date_updated: 2023-08-25T10:30:37Z
day: '10'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1371/journal.pgen.1008079
ec_funded: 1
external_id:
isi:
- '000466866000029'
file:
- access_level: open_access
checksum: cf3889c8a8a16053dacf9c3776cbe217
content_type: application/pdf
creator: dernst
date_created: 2019-05-14T08:26:08Z
date_updated: 2020-07-14T12:47:30Z
file_id: '6445'
file_name: 2019_PLOSGenetics_Pokusaeva.pdf
file_size: 3726017
relation: main_file
file_date_updated: 2020-07-14T12:47:30Z
has_accepted_license: '1'
intvolume: ' 15'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: PLoS Genetics
publication_identifier:
eissn:
- '15537404'
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
record:
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relation: research_data
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relation: research_data
status: public
- id: '9797'
relation: research_data
status: public
scopus_import: '1'
status: public
title: An experimental assay of the interactions of amino acids from orthologous sequences
shaping a complex fitness landscape
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 15
year: '2019'
...
---
_id: '9790'
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author:
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full_name: Pokusaeva, Victoria
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last_name: Pokusaeva
orcid: 0000-0001-7660-444X
- first_name: Dinara R.
full_name: Usmanova, Dinara R.
last_name: Usmanova
- first_name: Ekaterina V.
full_name: Putintseva, Ekaterina V.
last_name: Putintseva
- first_name: Lorena
full_name: Espinar, Lorena
last_name: Espinar
- first_name: Karen
full_name: Sarkisyan, Karen
id: 39A7BF80-F248-11E8-B48F-1D18A9856A87
last_name: Sarkisyan
orcid: 0000-0002-5375-6341
- first_name: Alexander S.
full_name: Mishin, Alexander S.
last_name: Mishin
- first_name: Natalya S.
full_name: Bogatyreva, Natalya S.
last_name: Bogatyreva
- first_name: Dmitry
full_name: Ivankov, Dmitry
id: 49FF1036-F248-11E8-B48F-1D18A9856A87
last_name: Ivankov
- first_name: Arseniy
full_name: Akopyan, Arseniy
id: 430D2C90-F248-11E8-B48F-1D18A9856A87
last_name: Akopyan
orcid: 0000-0002-2548-617X
- first_name: Sergey
full_name: Avvakumov, Sergey
id: 3827DAC8-F248-11E8-B48F-1D18A9856A87
last_name: Avvakumov
- first_name: Inna S.
full_name: Povolotskaya, Inna S.
last_name: Povolotskaya
- first_name: Guillaume J.
full_name: Filion, Guillaume J.
last_name: Filion
- first_name: Lucas B.
full_name: Carey, Lucas B.
last_name: Carey
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
citation:
ama: Pokusaeva V, Usmanova DR, Putintseva EV, et al. A statistical summary of segment
libraries and sequencing results. 2019. doi:10.1371/journal.pgen.1008079.s011
apa: Pokusaeva, V., Usmanova, D. R., Putintseva, E. V., Espinar, L., Sarkisyan,
K., Mishin, A. S., … Kondrashov, F. (2019). A statistical summary of segment libraries
and sequencing results. Public Library of Science. https://doi.org/10.1371/journal.pgen.1008079.s011
chicago: Pokusaeva, Victoria, Dinara R. Usmanova, Ekaterina V. Putintseva, Lorena
Espinar, Karen Sarkisyan, Alexander S. Mishin, Natalya S. Bogatyreva, et al. “A
Statistical Summary of Segment Libraries and Sequencing Results.” Public Library
of Science, 2019. https://doi.org/10.1371/journal.pgen.1008079.s011.
ieee: V. Pokusaeva et al., “A statistical summary of segment libraries and
sequencing results.” Public Library of Science, 2019.
ista: Pokusaeva V, Usmanova DR, Putintseva EV, Espinar L, Sarkisyan K, Mishin AS,
Bogatyreva NS, Ivankov D, Akopyan A, Avvakumov S, Povolotskaya IS, Filion GJ,
Carey LB, Kondrashov F. 2019. A statistical summary of segment libraries and sequencing
results, Public Library of Science, 10.1371/journal.pgen.1008079.s011.
mla: Pokusaeva, Victoria, et al. A Statistical Summary of Segment Libraries and
Sequencing Results. Public Library of Science, 2019, doi:10.1371/journal.pgen.1008079.s011.
short: V. Pokusaeva, D.R. Usmanova, E.V. Putintseva, L. Espinar, K. Sarkisyan, A.S.
Mishin, N.S. Bogatyreva, D. Ivankov, A. Akopyan, S. Avvakumov, I.S. Povolotskaya,
G.J. Filion, L.B. Carey, F. Kondrashov, (2019).
date_created: 2021-08-06T08:50:15Z
date_published: 2019-04-10T00:00:00Z
date_updated: 2023-08-25T10:30:36Z
day: '10'
department:
- _id: FyKo
doi: 10.1371/journal.pgen.1008079.s011
month: '04'
oa_version: Published Version
publisher: Public Library of Science
related_material:
record:
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relation: used_in_publication
status: public
status: public
title: A statistical summary of segment libraries and sequencing results
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
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...
---
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author:
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full_name: Pokusaeva, Victoria
id: 3184041C-F248-11E8-B48F-1D18A9856A87
last_name: Pokusaeva
orcid: 0000-0001-7660-444X
- first_name: Dinara R.
full_name: Usmanova, Dinara R.
last_name: Usmanova
- first_name: Ekaterina V.
full_name: Putintseva, Ekaterina V.
last_name: Putintseva
- first_name: Lorena
full_name: Espinar, Lorena
last_name: Espinar
- first_name: Karen
full_name: Sarkisyan, Karen
id: 39A7BF80-F248-11E8-B48F-1D18A9856A87
last_name: Sarkisyan
orcid: 0000-0002-5375-6341
- first_name: Alexander S.
full_name: Mishin, Alexander S.
last_name: Mishin
- first_name: Natalya S.
full_name: Bogatyreva, Natalya S.
last_name: Bogatyreva
- first_name: Dmitry
full_name: Ivankov, Dmitry
id: 49FF1036-F248-11E8-B48F-1D18A9856A87
last_name: Ivankov
- first_name: Arseniy
full_name: Akopyan, Arseniy
id: 430D2C90-F248-11E8-B48F-1D18A9856A87
last_name: Akopyan
orcid: 0000-0002-2548-617X
- first_name: Inna S.
full_name: Povolotskaya, Inna S.
last_name: Povolotskaya
- first_name: Guillaume J.
full_name: Filion, Guillaume J.
last_name: Filion
- first_name: Lucas B.
full_name: Carey, Lucas B.
last_name: Carey
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
citation:
ama: Pokusaeva V, Usmanova DR, Putintseva EV, et al. A statistical summary of segment
libraries and sequencing results. 2019. doi:10.1371/journal.pgen.1008079.s011
apa: Pokusaeva, V., Usmanova, D. R., Putintseva, E. V., Espinar, L., Sarkisyan,
K., Mishin, A. S., … Kondrashov, F. (2019). A statistical summary of segment libraries
and sequencing results. Public Library of Science. https://doi.org/10.1371/journal.pgen.1008079.s011
chicago: Pokusaeva, Victoria, Dinara R. Usmanova, Ekaterina V. Putintseva, Lorena
Espinar, Karen Sarkisyan, Alexander S. Mishin, Natalya S. Bogatyreva, et al. “A
Statistical Summary of Segment Libraries and Sequencing Results.” Public Library
of Science, 2019. https://doi.org/10.1371/journal.pgen.1008079.s011.
ieee: V. Pokusaeva et al., “A statistical summary of segment libraries and
sequencing results.” Public Library of Science, 2019.
ista: Pokusaeva V, Usmanova DR, Putintseva EV, Espinar L, Sarkisyan K, Mishin AS,
Bogatyreva NS, Ivankov D, Akopyan A, Povolotskaya IS, Filion GJ, Carey LB, Kondrashov
F. 2019. A statistical summary of segment libraries and sequencing results, Public
Library of Science, 10.1371/journal.pgen.1008079.s011.
mla: Pokusaeva, Victoria, et al. A Statistical Summary of Segment Libraries and
Sequencing Results. Public Library of Science, 2019, doi:10.1371/journal.pgen.1008079.s011.
short: V. Pokusaeva, D.R. Usmanova, E.V. Putintseva, L. Espinar, K. Sarkisyan, A.S.
Mishin, N.S. Bogatyreva, D. Ivankov, A. Akopyan, I.S. Povolotskaya, G.J. Filion,
L.B. Carey, F. Kondrashov, (2019).
date_created: 2021-08-06T11:08:20Z
date_published: 2019-04-10T00:00:00Z
date_updated: 2023-08-25T10:30:36Z
day: '10'
department:
- _id: FyKo
doi: 10.1371/journal.pgen.1008079.s011
month: '04'
oa_version: Published Version
publisher: Public Library of Science
related_material:
record:
- id: '6419'
relation: used_in_publication
status: public
status: public
title: A statistical summary of segment libraries and sequencing results
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9789'
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author:
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full_name: Pokusaeva, Victoria
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last_name: Pokusaeva
orcid: 0000-0001-7660-444X
- first_name: Dinara R.
full_name: Usmanova, Dinara R.
last_name: Usmanova
- first_name: Ekaterina V.
full_name: Putintseva, Ekaterina V.
last_name: Putintseva
- first_name: Lorena
full_name: Espinar, Lorena
last_name: Espinar
- first_name: Karen
full_name: Sarkisyan, Karen
id: 39A7BF80-F248-11E8-B48F-1D18A9856A87
last_name: Sarkisyan
orcid: 0000-0002-5375-6341
- first_name: Alexander S.
full_name: Mishin, Alexander S.
last_name: Mishin
- first_name: Natalya S.
full_name: Bogatyreva, Natalya S.
last_name: Bogatyreva
- first_name: Dmitry
full_name: Ivankov, Dmitry
id: 49FF1036-F248-11E8-B48F-1D18A9856A87
last_name: Ivankov
- first_name: Arseniy
full_name: Akopyan, Arseniy
id: 430D2C90-F248-11E8-B48F-1D18A9856A87
last_name: Akopyan
orcid: 0000-0002-2548-617X
- first_name: Sergey
full_name: Avvakumov, Sergey
id: 3827DAC8-F248-11E8-B48F-1D18A9856A87
last_name: Avvakumov
- first_name: Inna S.
full_name: Povolotskaya, Inna S.
last_name: Povolotskaya
- first_name: Guillaume J.
full_name: Filion, Guillaume J.
last_name: Filion
- first_name: Lucas B.
full_name: Carey, Lucas B.
last_name: Carey
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
citation:
ama: Pokusaeva V, Usmanova DR, Putintseva EV, et al. Multiple alignment of His3
orthologues. 2019. doi:10.1371/journal.pgen.1008079.s010
apa: Pokusaeva, V., Usmanova, D. R., Putintseva, E. V., Espinar, L., Sarkisyan,
K., Mishin, A. S., … Kondrashov, F. (2019). Multiple alignment of His3 orthologues.
Public Library of Science. https://doi.org/10.1371/journal.pgen.1008079.s010
chicago: Pokusaeva, Victoria, Dinara R. Usmanova, Ekaterina V. Putintseva, Lorena
Espinar, Karen Sarkisyan, Alexander S. Mishin, Natalya S. Bogatyreva, et al. “Multiple
Alignment of His3 Orthologues.” Public Library of Science, 2019. https://doi.org/10.1371/journal.pgen.1008079.s010.
ieee: V. Pokusaeva et al., “Multiple alignment of His3 orthologues.” Public
Library of Science, 2019.
ista: Pokusaeva V, Usmanova DR, Putintseva EV, Espinar L, Sarkisyan K, Mishin AS,
Bogatyreva NS, Ivankov D, Akopyan A, Avvakumov S, Povolotskaya IS, Filion GJ,
Carey LB, Kondrashov F. 2019. Multiple alignment of His3 orthologues, Public Library
of Science, 10.1371/journal.pgen.1008079.s010.
mla: Pokusaeva, Victoria, et al. Multiple Alignment of His3 Orthologues.
Public Library of Science, 2019, doi:10.1371/journal.pgen.1008079.s010.
short: V. Pokusaeva, D.R. Usmanova, E.V. Putintseva, L. Espinar, K. Sarkisyan, A.S.
Mishin, N.S. Bogatyreva, D. Ivankov, A. Akopyan, S. Avvakumov, I.S. Povolotskaya,
G.J. Filion, L.B. Carey, F. Kondrashov, (2019).
date_created: 2021-08-06T08:38:50Z
date_published: 2019-04-10T00:00:00Z
date_updated: 2023-08-25T10:30:36Z
day: '10'
department:
- _id: FyKo
doi: 10.1371/journal.pgen.1008079.s010
month: '04'
oa_version: Published Version
publisher: Public Library of Science
related_material:
record:
- id: '6419'
relation: used_in_publication
status: public
status: public
title: Multiple alignment of His3 orthologues
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '6506'
abstract:
- lang: eng
text: How does environmental complexity affect the evolution of single genes? Here,
we measured the effects of a set of Bacillus subtilis glutamate dehydrogenase
mutants across 19 different environments—from phenotypically homogeneous single-cell
populations in liquid media to heterogeneous biofilms, plant roots and soil populations.
The effects of individual gene mutations on organismal fitness were highly reproducible
in liquid cultures. However, 84% of the tested alleles showed opposing fitness
effects under different growth conditions (sign environmental pleiotropy). In
colony biofilms and soil samples, different alleles dominated in parallel replica
experiments. Accordingly, we found that in these heterogeneous cell populations
the fate of mutations was dictated by a combination of selection and drift. The
latter relates to programmed prophage excisions that occurred during biofilm development.
Overall, for each condition, a wide range of glutamate dehydrogenase mutations
persisted and sometimes fixated as a result of the combined action of selection,
pleiotropy and chance. However, over longer periods and in multiple environments,
nearly all of this diversity would be lost—across all the environments and conditions
that we tested, the wild type was the fittest allele.
article_processing_charge: No
article_type: original
author:
- first_name: Lianet
full_name: Noda-García, Lianet
last_name: Noda-García
- first_name: Dan
full_name: Davidi, Dan
last_name: Davidi
- first_name: Elisa
full_name: Korenblum, Elisa
last_name: Korenblum
- first_name: Assaf
full_name: Elazar, Assaf
last_name: Elazar
- first_name: Ekaterina
full_name: Putintseva, Ekaterina
id: 2EF67C84-F248-11E8-B48F-1D18A9856A87
last_name: Putintseva
- first_name: Asaph
full_name: Aharoni, Asaph
last_name: Aharoni
- first_name: Dan S.
full_name: Tawfik, Dan S.
last_name: Tawfik
citation:
ama: Noda-García L, Davidi D, Korenblum E, et al. Chance and pleiotropy dominate
genetic diversity in complex bacterial environments. Nature Microbiology.
2019;4(7):1221–1230. doi:10.1038/s41564-019-0412-y
apa: Noda-García, L., Davidi, D., Korenblum, E., Elazar, A., Putintseva, E., Aharoni,
A., & Tawfik, D. S. (2019). Chance and pleiotropy dominate genetic diversity
in complex bacterial environments. Nature Microbiology. Springer Nature.
https://doi.org/10.1038/s41564-019-0412-y
chicago: Noda-García, Lianet, Dan Davidi, Elisa Korenblum, Assaf Elazar, Ekaterina
Putintseva, Asaph Aharoni, and Dan S. Tawfik. “Chance and Pleiotropy Dominate
Genetic Diversity in Complex Bacterial Environments.” Nature Microbiology.
Springer Nature, 2019. https://doi.org/10.1038/s41564-019-0412-y.
ieee: L. Noda-García et al., “Chance and pleiotropy dominate genetic diversity
in complex bacterial environments,” Nature Microbiology, vol. 4, no. 7.
Springer Nature, pp. 1221–1230, 2019.
ista: Noda-García L, Davidi D, Korenblum E, Elazar A, Putintseva E, Aharoni A, Tawfik
DS. 2019. Chance and pleiotropy dominate genetic diversity in complex bacterial
environments. Nature Microbiology. 4(7), 1221–1230.
mla: Noda-García, Lianet, et al. “Chance and Pleiotropy Dominate Genetic Diversity
in Complex Bacterial Environments.” Nature Microbiology, vol. 4, no. 7,
Springer Nature, 2019, pp. 1221–1230, doi:10.1038/s41564-019-0412-y.
short: L. Noda-García, D. Davidi, E. Korenblum, A. Elazar, E. Putintseva, A. Aharoni,
D.S. Tawfik, Nature Microbiology 4 (2019) 1221–1230.
date_created: 2019-05-29T13:03:30Z
date_published: 2019-07-01T00:00:00Z
date_updated: 2023-08-28T08:39:47Z
day: '01'
department:
- _id: FyKo
doi: 10.1038/s41564-019-0412-y
external_id:
isi:
- '000480348200017'
intvolume: ' 4'
isi: 1
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.biorxiv.org/content/10.1101/340828v2
month: '07'
oa: 1
oa_version: Preprint
page: 1221–1230
publication: Nature Microbiology
publication_identifier:
issn:
- 2058-5276
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Chance and pleiotropy dominate genetic diversity in complex bacterial environments
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 4
year: '2019'
...
---
_id: '9731'
abstract:
- lang: eng
text: OGs with putative pseudogenes by the number of affected genomes in different
chlamydial species. Frameshift and nonsense mutations located less than 60 bp
upstreamof the gene end or present in a single genome from the corresponding OG
were excluded. (CSV 31 kb)
article_processing_charge: No
author:
- first_name: Olga
full_name: Sigalova, Olga
last_name: Sigalova
- first_name: Andrei
full_name: Chaplin, Andrei
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel
full_name: Shelyakin, Pavel
last_name: Shelyakin
- first_name: Vsevolod
full_name: Filaretov, Vsevolod
last_name: Filaretov
- first_name: Evgeny
full_name: Akkuratov, Evgeny
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova O, Chaplin A, Bochkareva O, et al. Additional file 11 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808772.v1
apa: Sigalova, O., Chaplin, A., Bochkareva, O., Shelyakin, P., Filaretov, V., Akkuratov,
E., … Gelfand, M. S. (2019). Additional file 11 of Chlamydia pan-genomic analysis
reveals balance between host adaptation and selective pressure to genome reduction.
Springer Nature. https://doi.org/10.6084/m9.figshare.9808772.v1
chicago: Sigalova, Olga, Andrei Chaplin, Olga Bochkareva, Pavel Shelyakin, Vsevolod
Filaretov, Evgeny Akkuratov, Valentina Burskaia, and Mikhail S. Gelfand. “Additional
File 11 of Chlamydia Pan-Genomic Analysis Reveals Balance between Host Adaptation
and Selective Pressure to Genome Reduction.” Springer Nature, 2019. https://doi.org/10.6084/m9.figshare.9808772.v1.
ieee: O. Sigalova et al., “Additional file 11 of Chlamydia pan-genomic analysis
reveals balance between host adaptation and selective pressure to genome reduction.”
Springer Nature, 2019.
ista: Sigalova O, Chaplin A, Bochkareva O, Shelyakin P, Filaretov V, Akkuratov E,
Burskaia V, Gelfand MS. 2019. Additional file 11 of Chlamydia pan-genomic analysis
reveals balance between host adaptation and selective pressure to genome reduction,
Springer Nature, 10.6084/m9.figshare.9808772.v1.
mla: Sigalova, Olga, et al. Additional File 11 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808772.v1.
short: O. Sigalova, A. Chaplin, O. Bochkareva, P. Shelyakin, V. Filaretov, E. Akkuratov,
V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-07-27T14:09:11Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:21Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808772.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808772.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 11 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9783'
abstract:
- lang: eng
text: Predicted frameshift and nonsense mutations in Chlamydial pan-genome. For
the analysis of putative pseudogenes, events located less than 60 bp. away from
gene end or present in a single genome from the corresponding OG were excluded.
(CSV 600 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 10 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808760.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 10 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808760.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 10 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808760.v1.
ieee: O. M. Sigalova et al., “Additional file 10 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 10 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction, Springer Nature, 10.6084/m9.figshare.9808760.v1.
mla: Sigalova, Olga M., et al. Additional File 10 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808760.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-06T07:59:56Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:21Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808760.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808760.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 10 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9897'
abstract:
- lang: eng
text: Frameshift and nonsense mutations near homopolymeric tracts of OG1 genes.
Only 374 genes with typical length and domain composition were considered. (CSV
6 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 20 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808850.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 20 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808850.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 20 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808850.v1.
ieee: O. M. Sigalova et al., “Additional file 20 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 20 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction, Springer Nature, 10.6084/m9.figshare.9808850.v1.
mla: Sigalova, Olga M., et al. Additional File 20 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808850.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T07:58:15Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:21Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808850.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808850.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 20 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9890'
abstract:
- lang: eng
text: Distribution of OGs with mosaic phyletic patterns across species (complete
genomes only). (CSV 7 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 15 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808802.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 15 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808802.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 15 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808802.v1.
ieee: O. M. Sigalova et al., “Additional file 15 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 15 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction, Springer Nature, 10.6084/m9.figshare.9808802.v1.
mla: Sigalova, Olga M., et al. Additional File 15 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808802.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-11T14:26:40Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:21Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808802.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808802.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 15 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9892'
abstract:
- lang: eng
text: Distribution of OGs with mosaic phyletic patterns across species (all genomes).
(CSV 10 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V
full_name: Chaplin, Andrei V
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 16 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808814.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 16 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808814.v1
chicago: Sigalova, Olga M., Andrei V Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 16 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808814.v1.
ieee: O. M. Sigalova et al., “Additional file 16 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 16 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction, Springer Nature, 10.6084/m9.figshare.9808814.v1.
mla: Sigalova, Olga M., et al. Additional File 16 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808814.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T07:11:53Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:21Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808814.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808814.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 16 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9893'
abstract:
- lang: eng
text: Summary of peripheral genesa phyletic patterns and tree concordance. (CSV
26 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 17 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808820.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 17 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808820.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 17 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808820.v1.
ieee: O. M. Sigalova et al., “Additional file 17 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 17 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction, Springer Nature, 10.6084/m9.figshare.9808820.v1.
mla: Sigalova, Olga M., et al. Additional File 17 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808820.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T07:20:10Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:21Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808820.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808820.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 17 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9894'
abstract:
- lang: eng
text: Orthologous families (OFs) derived by MCL clustering of OGs. (CSV 189 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 18 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808826.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 18 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808826.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 18 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808826.v1.
ieee: O. M. Sigalova et al., “Additional file 18 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 18 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction, Springer Nature, 10.6084/m9.figshare.9808826.v1.
mla: Sigalova, Olga M., et al. Additional File 18 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808826.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T07:25:07Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:21Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808826.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808826.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 18 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9895'
abstract:
- lang: eng
text: Additional information on proteins from OG1. (CSV 30 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 19 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808835.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 19 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808835.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 19 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808835.v1.
ieee: O. M. Sigalova et al., “Additional file 19 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 19 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction, Springer Nature, 10.6084/m9.figshare.9808835.v1.
mla: Sigalova, Olga M., et al. Additional File 19 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808835.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T07:44:52Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:21Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808835.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808835.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 19 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9896'
abstract:
- lang: eng
text: Summary of the analysed genomes. (CSV 24 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 1 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808841.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 1 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808841.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 1 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808841.v1.
ieee: O. M. Sigalova et al., “Additional file 1 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 1 of Chlamydia pan-genomic analysis
reveals balance between host adaptation and selective pressure to genome reduction,
Springer Nature, 10.6084/m9.figshare.9808841.v1.
mla: Sigalova, Olga M., et al. Additional File 1 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808841.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T07:50:53Z
date_published: 2019-09-02T00:00:00Z
date_updated: 2023-08-30T06:20:21Z
day: '02'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808841.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808841.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 1 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '6898'
abstract:
- lang: eng
text: "Background\r\n\r\nChlamydia are ancient intracellular pathogens with reduced,
though strikingly conserved genome. Despite their parasitic lifestyle and isolated
intracellular environment, these bacteria managed to avoid accumulation of deleterious
mutations leading to subsequent genome degradation characteristic for many parasitic
bacteria.\r\nResults\r\n\r\nWe report pan-genomic analysis of sixteen species
from genus Chlamydia including identification and functional annotation of orthologous
genes, and characterization of gene gains, losses, and rearrangements. We demonstrate
the overall genome stability of these bacteria as indicated by a large fraction
of common genes with conserved genomic locations. On the other hand, extreme evolvability
is confined to several paralogous gene families such as polymorphic membrane proteins
and phospholipase D, and likely is caused by the pressure from the host immune
system.\r\nConclusions\r\n\r\nThis combination of a large, conserved core genome
and a small, evolvable periphery likely reflect the balance between the selective
pressure towards genome reduction and the need to adapt to escape from the host
immunity."
article_number: '710'
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Chlamydia pan-genomic analysis
reveals balance between host adaptation and selective pressure to genome reduction.
BMC Genomics. 2019;20(1). doi:10.1186/s12864-019-6059-5
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Chlamydia pan-genomic analysis
reveals balance between host adaptation and selective pressure to genome reduction.
BMC Genomics. BioMed Central. https://doi.org/10.1186/s12864-019-6059-5
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Chlamydia Pan-Genomic Analysis Reveals Balance between Host Adaptation
and Selective Pressure to Genome Reduction.” BMC Genomics. BioMed Central,
2019. https://doi.org/10.1186/s12864-019-6059-5.
ieee: O. M. Sigalova et al., “Chlamydia pan-genomic analysis reveals balance
between host adaptation and selective pressure to genome reduction,” BMC Genomics,
vol. 20, no. 1. BioMed Central, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Chlamydia pan-genomic analysis reveals balance
between host adaptation and selective pressure to genome reduction. BMC Genomics.
20(1), 710.
mla: Sigalova, Olga M., et al. “Chlamydia Pan-Genomic Analysis Reveals Balance between
Host Adaptation and Selective Pressure to Genome Reduction.” BMC Genomics,
vol. 20, no. 1, 710, BioMed Central, 2019, doi:10.1186/s12864-019-6059-5.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, BMC Genomics 20 (2019).
date_created: 2019-09-22T22:00:36Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:22Z
day: '12'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1186/s12864-019-6059-5
external_id:
isi:
- '000485256100001'
file:
- access_level: open_access
checksum: b798773c5823012d31c812c9f7975da2
content_type: application/pdf
creator: kschuh
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date_updated: 2020-07-14T12:47:44Z
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file_name: 2019_BioMed_Sigalova.pdf
file_size: 4157175
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has_accepted_license: '1'
intvolume: ' 20'
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issue: '1'
language:
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month: '09'
oa: 1
oa_version: Published Version
publication: BMC Genomics
publication_identifier:
eissn:
- '14712164'
publication_status: published
publisher: BioMed Central
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- id: '9893'
relation: research_data
status: public
- id: '9894'
relation: research_data
status: public
- id: '9895'
relation: research_data
status: public
- id: '9896'
relation: research_data
status: public
- id: '9897'
relation: research_data
status: public
- id: '9898'
relation: research_data
status: public
- id: '9899'
relation: research_data
status: public
- id: '9900'
relation: research_data
status: public
- id: '9901'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Chlamydia pan-genomic analysis reveals balance between host adaptation and
selective pressure to genome reduction
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 20
year: '2019'
...
---
_id: '9898'
abstract:
- lang: eng
text: All polyN tracts of length 5 or more nucleotides in sequences of genes from
OG1. Sequences were extracted and scanned prior to automatic correction for frameshifts
implemented in the RAST pipeline. (CSV 133 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 21 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808859.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 21 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808859.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 21 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808859.v1.
ieee: O. M. Sigalova et al., “Additional file 21 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 21 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction, Springer Nature, 10.6084/m9.figshare.9808859.v1.
mla: Sigalova, Olga M., et al. Additional File 21 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808859.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T08:10:23Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:22Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808859.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808859.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 21 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9901'
abstract:
- lang: eng
text: Clusters of Orthologous Genes (COGs) and corresponding functional categories
assigned to OGs. (CSV 117 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 9 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808907.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 9 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808907.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 9 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808907.v1.
ieee: O. M. Sigalova et al., “Additional file 9 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 9 of Chlamydia pan-genomic analysis
reveals balance between host adaptation and selective pressure to genome reduction,
Springer Nature, 10.6084/m9.figshare.9808907.v1.
mla: Sigalova, Olga M., et al. Additional File 9 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808907.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T10:54:03Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:22Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808907.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808907.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 9 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9899'
abstract:
- lang: eng
text: Summary of orthologous groups (OGs) for 227 genomes of genus Chlamydia. (CSV
362 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 2 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808865.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 2 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808865.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 2 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808865.v1.
ieee: O. M. Sigalova et al., “Additional file 2 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 2 of Chlamydia pan-genomic analysis
reveals balance between host adaptation and selective pressure to genome reduction,
Springer Nature, 10.6084/m9.figshare.9808865.v1.
mla: Sigalova, Olga M., et al. Additional File 2 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808865.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T08:18:09Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:22Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808865.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808865.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 2 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '9900'
abstract:
- lang: eng
text: Pan-genome statistics by species. (CSV 3 kb)
article_processing_charge: No
author:
- first_name: Olga M.
full_name: Sigalova, Olga M.
last_name: Sigalova
- first_name: Andrei V.
full_name: Chaplin, Andrei V.
last_name: Chaplin
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Pavel V.
full_name: Shelyakin, Pavel V.
last_name: Shelyakin
- first_name: Vsevolod A.
full_name: Filaretov, Vsevolod A.
last_name: Filaretov
- first_name: Evgeny E.
full_name: Akkuratov, Evgeny E.
last_name: Akkuratov
- first_name: Valentina
full_name: Burskaia, Valentina
last_name: Burskaia
- first_name: Mikhail S.
full_name: Gelfand, Mikhail S.
last_name: Gelfand
citation:
ama: Sigalova OM, Chaplin AV, Bochkareva O, et al. Additional file 5 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. 2019. doi:10.6084/m9.figshare.9808886.v1
apa: Sigalova, O. M., Chaplin, A. V., Bochkareva, O., Shelyakin, P. V., Filaretov,
V. A., Akkuratov, E. E., … Gelfand, M. S. (2019). Additional file 5 of Chlamydia
pan-genomic analysis reveals balance between host adaptation and selective pressure
to genome reduction. Springer Nature. https://doi.org/10.6084/m9.figshare.9808886.v1
chicago: Sigalova, Olga M., Andrei V. Chaplin, Olga Bochkareva, Pavel V. Shelyakin,
Vsevolod A. Filaretov, Evgeny E. Akkuratov, Valentina Burskaia, and Mikhail S.
Gelfand. “Additional File 5 of Chlamydia Pan-Genomic Analysis Reveals Balance
between Host Adaptation and Selective Pressure to Genome Reduction.” Springer
Nature, 2019. https://doi.org/10.6084/m9.figshare.9808886.v1.
ieee: O. M. Sigalova et al., “Additional file 5 of Chlamydia pan-genomic
analysis reveals balance between host adaptation and selective pressure to genome
reduction.” Springer Nature, 2019.
ista: Sigalova OM, Chaplin AV, Bochkareva O, Shelyakin PV, Filaretov VA, Akkuratov
EE, Burskaia V, Gelfand MS. 2019. Additional file 5 of Chlamydia pan-genomic analysis
reveals balance between host adaptation and selective pressure to genome reduction,
Springer Nature, 10.6084/m9.figshare.9808886.v1.
mla: Sigalova, Olga M., et al. Additional File 5 of Chlamydia Pan-Genomic Analysis
Reveals Balance between Host Adaptation and Selective Pressure to Genome Reduction.
Springer Nature, 2019, doi:10.6084/m9.figshare.9808886.v1.
short: O.M. Sigalova, A.V. Chaplin, O. Bochkareva, P.V. Shelyakin, V.A. Filaretov,
E.E. Akkuratov, V. Burskaia, M.S. Gelfand, (2019).
date_created: 2021-08-12T08:44:49Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2023-08-30T06:20:22Z
day: '12'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.9808886.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9808886.v1
month: '09'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6898'
relation: used_in_publication
status: public
status: public
title: Additional file 5 of Chlamydia pan-genomic analysis reveals balance between
host adaptation and selective pressure to genome reduction
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '7181'
abstract:
- lang: eng
text: Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary
predictions1,2, but the complexity of aligning large datasets requires the use
of approximate solutions3, including the progressive algorithm4. Progressive MSA
methods start by aligning the most similar sequences and subsequently incorporate
the remaining sequences, from leaf-to-root, based on a guide-tree. Their accuracy
declines substantially as the number of sequences is scaled up5. We introduce
a regressive algorithm that enables MSA of up to 1.4 million sequences on a standard
workstation and substantially improves accuracy on datasets larger than 10,000
sequences. Our regressive algorithm works the other way around to the progressive
algorithm and begins by aligning the most dissimilar sequences. It uses an efficient
divide-and-conquer strategy to run third-party alignment methods in linear time,
regardless of their original complexity. Our approach will enable analyses of
extremely large genomic datasets such as the recently announced Earth BioGenome
Project, which comprises 1.5 million eukaryotic genomes6.
article_processing_charge: No
article_type: original
author:
- first_name: Edgar
full_name: Garriga, Edgar
last_name: Garriga
- first_name: Paolo
full_name: Di Tommaso, Paolo
last_name: Di Tommaso
- first_name: Cedrik
full_name: Magis, Cedrik
last_name: Magis
- first_name: Ionas
full_name: Erb, Ionas
last_name: Erb
- first_name: Leila
full_name: Mansouri, Leila
last_name: Mansouri
- first_name: Athanasios
full_name: Baltzis, Athanasios
last_name: Baltzis
- first_name: Hafid
full_name: Laayouni, Hafid
last_name: Laayouni
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Evan
full_name: Floden, Evan
last_name: Floden
- first_name: Cedric
full_name: Notredame, Cedric
last_name: Notredame
citation:
ama: Garriga E, Di Tommaso P, Magis C, et al. Large multiple sequence alignments
with a root-to-leaf regressive method. Nature Biotechnology. 2019;37(12):1466-1470.
doi:10.1038/s41587-019-0333-6
apa: Garriga, E., Di Tommaso, P., Magis, C., Erb, I., Mansouri, L., Baltzis, A.,
… Notredame, C. (2019). Large multiple sequence alignments with a root-to-leaf
regressive method. Nature Biotechnology. Springer Nature. https://doi.org/10.1038/s41587-019-0333-6
chicago: Garriga, Edgar, Paolo Di Tommaso, Cedrik Magis, Ionas Erb, Leila Mansouri,
Athanasios Baltzis, Hafid Laayouni, Fyodor Kondrashov, Evan Floden, and Cedric
Notredame. “Large Multiple Sequence Alignments with a Root-to-Leaf Regressive
Method.” Nature Biotechnology. Springer Nature, 2019. https://doi.org/10.1038/s41587-019-0333-6.
ieee: E. Garriga et al., “Large multiple sequence alignments with a root-to-leaf
regressive method,” Nature Biotechnology, vol. 37, no. 12. Springer Nature,
pp. 1466–1470, 2019.
ista: Garriga E, Di Tommaso P, Magis C, Erb I, Mansouri L, Baltzis A, Laayouni H,
Kondrashov F, Floden E, Notredame C. 2019. Large multiple sequence alignments
with a root-to-leaf regressive method. Nature Biotechnology. 37(12), 1466–1470.
mla: Garriga, Edgar, et al. “Large Multiple Sequence Alignments with a Root-to-Leaf
Regressive Method.” Nature Biotechnology, vol. 37, no. 12, Springer Nature,
2019, pp. 1466–70, doi:10.1038/s41587-019-0333-6.
short: E. Garriga, P. Di Tommaso, C. Magis, I. Erb, L. Mansouri, A. Baltzis, H.
Laayouni, F. Kondrashov, E. Floden, C. Notredame, Nature Biotechnology 37 (2019)
1466–1470.
date_created: 2019-12-15T23:00:43Z
date_published: 2019-12-01T00:00:00Z
date_updated: 2023-09-06T14:32:52Z
day: '01'
department:
- _id: FyKo
doi: 10.1038/s41587-019-0333-6
ec_funded: 1
external_id:
isi:
- '000500748900021'
pmid:
- '31792410'
intvolume: ' 37'
isi: 1
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894943/
month: '12'
oa: 1
oa_version: Submitted Version
page: 1466-1470
pmid: 1
project:
- _id: 26580278-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '771209'
name: Characterizing the fitness landscape on population and global scales
publication: Nature Biotechnology
publication_identifier:
eissn:
- '15461696'
issn:
- '10870156'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '13059'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Large multiple sequence alignments with a root-to-leaf regressive method
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 37
year: '2019'
...
---
_id: '13059'
abstract:
- lang: eng
text: "This dataset contains a GitHub repository containing all the data, analysis,
Nextflow workflows and Jupyter notebooks to replicate the manuscript titled \"Fast
and accurate large multiple sequence alignments with a root-to-leaf regressive
method\".\r\nIt also contains the Multiple Sequence Alignments (MSAs) generated
and well as the main figures and tables from the manuscript.\r\nThe repository
is also available at GitHub (https://github.com/cbcrg/dpa-analysis) release `v1.2`.\r\nFor
details on how to use the regressive alignment algorithm, see the T-Coffee software
suite (https://github.com/cbcrg/tcoffee)."
article_processing_charge: No
author:
- first_name: Edgar
full_name: Garriga, Edgar
last_name: Garriga
- first_name: Paolo
full_name: di Tommaso, Paolo
last_name: di Tommaso
- first_name: Cedrik
full_name: Magis, Cedrik
last_name: Magis
- first_name: Ionas
full_name: Erb, Ionas
last_name: Erb
- first_name: Leila
full_name: Mansouri, Leila
last_name: Mansouri
- first_name: Athanasios
full_name: Baltzis, Athanasios
last_name: Baltzis
- first_name: Hafid
full_name: Laayouni, Hafid
last_name: Laayouni
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Evan
full_name: Floden, Evan
last_name: Floden
- first_name: Cedric
full_name: Notredame, Cedric
last_name: Notredame
citation:
ama: Garriga E, di Tommaso P, Magis C, et al. Fast and accurate large multiple sequence
alignments with a root-to-leaf regressive method. 2018. doi:10.5281/ZENODO.2025846
apa: Garriga, E., di Tommaso, P., Magis, C., Erb, I., Mansouri, L., Baltzis, A.,
… Notredame, C. (2018). Fast and accurate large multiple sequence alignments with
a root-to-leaf regressive method. Zenodo. https://doi.org/10.5281/ZENODO.2025846
chicago: Garriga, Edgar, Paolo di Tommaso, Cedrik Magis, Ionas Erb, Leila Mansouri,
Athanasios Baltzis, Hafid Laayouni, Fyodor Kondrashov, Evan Floden, and Cedric
Notredame. “Fast and Accurate Large Multiple Sequence Alignments with a Root-to-Leaf
Regressive Method.” Zenodo, 2018. https://doi.org/10.5281/ZENODO.2025846.
ieee: E. Garriga et al., “Fast and accurate large multiple sequence alignments
with a root-to-leaf regressive method.” Zenodo, 2018.
ista: Garriga E, di Tommaso P, Magis C, Erb I, Mansouri L, Baltzis A, Laayouni H,
Kondrashov F, Floden E, Notredame C. 2018. Fast and accurate large multiple sequence
alignments with a root-to-leaf regressive method, Zenodo, 10.5281/ZENODO.2025846.
mla: Garriga, Edgar, et al. Fast and Accurate Large Multiple Sequence Alignments
with a Root-to-Leaf Regressive Method. Zenodo, 2018, doi:10.5281/ZENODO.2025846.
short: E. Garriga, P. di Tommaso, C. Magis, I. Erb, L. Mansouri, A. Baltzis, H.
Laayouni, F. Kondrashov, E. Floden, C. Notredame, (2018).
date_created: 2023-05-23T16:08:20Z
date_published: 2018-12-07T00:00:00Z
date_updated: 2023-09-06T14:32:51Z
day: '07'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.5281/ZENODO.2025846
main_file_link:
- open_access: '1'
url: https://doi.org/10.5281/zenodo.3271452
month: '12'
oa: 1
oa_version: Published Version
publisher: Zenodo
related_material:
record:
- id: '7181'
relation: used_in_publication
status: public
status: public
title: Fast and accurate large multiple sequence alignments with a root-to-leaf regressive
method
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data_reference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2018'
...
---
_id: '384'
abstract:
- lang: eng
text: Can orthologous proteins differ in terms of their ability to be secreted?
To answer this question, we investigated the distribution of signal peptides within
the orthologous groups of Enterobacterales. Parsimony analysis and sequence comparisons
revealed a large number of signal peptide gain and loss events, in which signal
peptides emerge or disappear in the course of evolution. Signal peptide losses
prevail over gains, an effect which is especially pronounced in the transition
from the free-living or commensal to the endosymbiotic lifestyle. The disproportionate
decline in the number of signal peptide-containing proteins in endosymbionts cannot
be explained by the overall reduction of their genomes. Signal peptides can be
gained and lost either by acquisition/elimination of the corresponding N-terminal
regions or by gradual accumulation of mutations. The evolutionary dynamics of
signal peptides in bacterial proteins represents a powerful mechanism of functional
diversification.
acknowledgement: "his work was supported by the Deutsche Forschungsgemeinschaft (grant
\ number FR 1411/9-1). This work was supported by the German Research Foundation
(DFG) and the Technical University of Munich within the fund- ing programme Open
Access Publish\r\nWe thank Goar Frishman for help with the annotation of the\r\nsymbiont
status of the organisms and Michael Galperin for\r\nuseful comments. T"
article_processing_charge: No
author:
- first_name: Peter
full_name: Hönigschmid, Peter
last_name: Hönigschmid
- first_name: Nadya
full_name: Bykova, Nadya
last_name: Bykova
- first_name: René
full_name: Schneider, René
last_name: Schneider
- first_name: Dmitry
full_name: Ivankov, Dmitry
id: 49FF1036-F248-11E8-B48F-1D18A9856A87
last_name: Ivankov
- first_name: Dmitrij
full_name: Frishman, Dmitrij
last_name: Frishman
citation:
ama: Hönigschmid P, Bykova N, Schneider R, Ivankov D, Frishman D. Evolutionary interplay
between symbiotic relationships and patterns of signal peptide gain and loss.
Genome Biology and Evolution. 2018;10(3):928-938. doi:10.1093/gbe/evy049
apa: Hönigschmid, P., Bykova, N., Schneider, R., Ivankov, D., & Frishman, D.
(2018). Evolutionary interplay between symbiotic relationships and patterns of
signal peptide gain and loss. Genome Biology and Evolution. Oxford University
Press. https://doi.org/10.1093/gbe/evy049
chicago: Hönigschmid, Peter, Nadya Bykova, René Schneider, Dmitry Ivankov, and Dmitrij
Frishman. “Evolutionary Interplay between Symbiotic Relationships and Patterns
of Signal Peptide Gain and Loss.” Genome Biology and Evolution. Oxford
University Press, 2018. https://doi.org/10.1093/gbe/evy049.
ieee: P. Hönigschmid, N. Bykova, R. Schneider, D. Ivankov, and D. Frishman, “Evolutionary
interplay between symbiotic relationships and patterns of signal peptide gain
and loss,” Genome Biology and Evolution, vol. 10, no. 3. Oxford University
Press, pp. 928–938, 2018.
ista: Hönigschmid P, Bykova N, Schneider R, Ivankov D, Frishman D. 2018. Evolutionary
interplay between symbiotic relationships and patterns of signal peptide gain
and loss. Genome Biology and Evolution. 10(3), 928–938.
mla: Hönigschmid, Peter, et al. “Evolutionary Interplay between Symbiotic Relationships
and Patterns of Signal Peptide Gain and Loss.” Genome Biology and Evolution,
vol. 10, no. 3, Oxford University Press, 2018, pp. 928–38, doi:10.1093/gbe/evy049.
short: P. Hönigschmid, N. Bykova, R. Schneider, D. Ivankov, D. Frishman, Genome
Biology and Evolution 10 (2018) 928–938.
date_created: 2018-12-11T11:46:10Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2023-09-11T13:56:52Z
day: '01'
ddc:
- '576'
department:
- _id: FyKo
doi: 10.1093/gbe/evy049
external_id:
isi:
- '000429483700022'
file:
- access_level: open_access
checksum: 458a7c2c2e79528567edfeb0f326cbe0
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:08:07Z
date_updated: 2020-07-14T12:46:16Z
file_id: '4667'
file_name: IST-2018-999-v1+1_2018_Ivankov_Evolutionary_interplay.pdf
file_size: 691602
relation: main_file
file_date_updated: 2020-07-14T12:46:16Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 928 - 938
publication: Genome Biology and Evolution
publication_status: published
publisher: Oxford University Press
publist_id: '7445'
pubrep_id: '999'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Evolutionary interplay between symbiotic relationships and patterns of signal
peptide gain and loss
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 10
year: '2018'
...
---
_id: '5780'
abstract:
- lang: eng
text: Bioluminescence is found across the entire tree of life, conferring a spectacular
set of visually oriented functions from attracting mates to scaring off predators.
Half a dozen different luciferins, molecules that emit light when enzymatically
oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis
has been described in full, which is found only in bacteria. Here, we report identification
of the fungal luciferase and three other key enzymes that together form the biosynthetic
cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite.
Introduction of the identified genes into the genome of the yeast Pichia pastoris
along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent
in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis
cycle and found that fungal bioluminescence emerged through a series of events
that included two independent gene duplications. The retention of the duplicated
enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication
was followed by functional sequence divergence of enzymes of at least one gene
in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence
proceeded through several closely related stepping stone nonluminescent biochemical
reactions with adaptive roles. The availability of a complete eukaryotic luciferin
biosynthesis pathway provides several applications in biomedicine and bioengineering.
article_processing_charge: No
author:
- first_name: Alexey A.
full_name: Kotlobay, Alexey A.
last_name: Kotlobay
- first_name: Karen
full_name: Sarkisyan, Karen
id: 39A7BF80-F248-11E8-B48F-1D18A9856A87
last_name: Sarkisyan
orcid: 0000-0002-5375-6341
- first_name: Yuliana A.
full_name: Mokrushina, Yuliana A.
last_name: Mokrushina
- first_name: Marina
full_name: Marcet-Houben, Marina
last_name: Marcet-Houben
- first_name: Ekaterina O.
full_name: Serebrovskaya, Ekaterina O.
last_name: Serebrovskaya
- first_name: Nadezhda M.
full_name: Markina, Nadezhda M.
last_name: Markina
- first_name: Louisa
full_name: Gonzalez Somermeyer, Louisa
id: 4720D23C-F248-11E8-B48F-1D18A9856A87
last_name: Gonzalez Somermeyer
orcid: 0000-0001-9139-5383
- first_name: Andrey Y.
full_name: Gorokhovatsky, Andrey Y.
last_name: Gorokhovatsky
- first_name: Andrey
full_name: Vvedensky, Andrey
last_name: Vvedensky
- first_name: Konstantin V.
full_name: Purtov, Konstantin V.
last_name: Purtov
- first_name: Valentin N.
full_name: Petushkov, Valentin N.
last_name: Petushkov
- first_name: Natalja S.
full_name: Rodionova, Natalja S.
last_name: Rodionova
- first_name: Tatiana V.
full_name: Chepurnyh, Tatiana V.
last_name: Chepurnyh
- first_name: Liliia
full_name: Fakhranurova, Liliia
last_name: Fakhranurova
- first_name: Elena B.
full_name: Guglya, Elena B.
last_name: Guglya
- first_name: Rustam
full_name: Ziganshin, Rustam
last_name: Ziganshin
- first_name: Aleksandra S.
full_name: Tsarkova, Aleksandra S.
last_name: Tsarkova
- first_name: Zinaida M.
full_name: Kaskova, Zinaida M.
last_name: Kaskova
- first_name: Victoria
full_name: Shender, Victoria
last_name: Shender
- first_name: Maxim
full_name: Abakumov, Maxim
last_name: Abakumov
- first_name: Tatiana O.
full_name: Abakumova, Tatiana O.
last_name: Abakumova
- first_name: Inna S.
full_name: Povolotskaya, Inna S.
last_name: Povolotskaya
- first_name: Fedor M.
full_name: Eroshkin, Fedor M.
last_name: Eroshkin
- first_name: Andrey G.
full_name: Zaraisky, Andrey G.
last_name: Zaraisky
- first_name: Alexander S.
full_name: Mishin, Alexander S.
last_name: Mishin
- first_name: Sergey V.
full_name: Dolgov, Sergey V.
last_name: Dolgov
- first_name: Tatiana Y.
full_name: Mitiouchkina, Tatiana Y.
last_name: Mitiouchkina
- first_name: Eugene P.
full_name: Kopantzev, Eugene P.
last_name: Kopantzev
- first_name: Hans E.
full_name: Waldenmaier, Hans E.
last_name: Waldenmaier
- first_name: Anderson G.
full_name: Oliveira, Anderson G.
last_name: Oliveira
- first_name: Yuichi
full_name: Oba, Yuichi
last_name: Oba
- first_name: Ekaterina
full_name: Barsova, Ekaterina
last_name: Barsova
- first_name: Ekaterina A.
full_name: Bogdanova, Ekaterina A.
last_name: Bogdanova
- first_name: Toni
full_name: Gabaldón, Toni
last_name: Gabaldón
- first_name: Cassius V.
full_name: Stevani, Cassius V.
last_name: Stevani
- first_name: Sergey
full_name: Lukyanov, Sergey
last_name: Lukyanov
- first_name: Ivan V.
full_name: Smirnov, Ivan V.
last_name: Smirnov
- first_name: Josef I.
full_name: Gitelson, Josef I.
last_name: Gitelson
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Ilia V.
full_name: Yampolsky, Ilia V.
last_name: Yampolsky
citation:
ama: Kotlobay AA, Sarkisyan K, Mokrushina YA, et al. Genetically encodable bioluminescent
system from fungi. Proceedings of the National Academy of Sciences of the United
States of America. 2018;115(50):12728-12732. doi:10.1073/pnas.1803615115
apa: Kotlobay, A. A., Sarkisyan, K., Mokrushina, Y. A., Marcet-Houben, M., Serebrovskaya,
E. O., Markina, N. M., … Yampolsky, I. V. (2018). Genetically encodable bioluminescent
system from fungi. Proceedings of the National Academy of Sciences of the United
States of America. National Academy of Sciences. https://doi.org/10.1073/pnas.1803615115
chicago: Kotlobay, Alexey A., Karen Sarkisyan, Yuliana A. Mokrushina, Marina Marcet-Houben,
Ekaterina O. Serebrovskaya, Nadezhda M. Markina, Louisa Gonzalez Somermeyer, et
al. “Genetically Encodable Bioluminescent System from Fungi.” Proceedings of
the National Academy of Sciences of the United States of America. National
Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1803615115.
ieee: A. A. Kotlobay et al., “Genetically encodable bioluminescent system
from fungi,” Proceedings of the National Academy of Sciences of the United
States of America, vol. 115, no. 50. National Academy of Sciences, pp. 12728–12732,
2018.
ista: Kotlobay AA, Sarkisyan K, Mokrushina YA, Marcet-Houben M, Serebrovskaya EO,
Markina NM, Gonzalez Somermeyer L, Gorokhovatsky AY, Vvedensky A, Purtov KV, Petushkov
VN, Rodionova NS, Chepurnyh TV, Fakhranurova L, Guglya EB, Ziganshin R, Tsarkova
AS, Kaskova ZM, Shender V, Abakumov M, Abakumova TO, Povolotskaya IS, Eroshkin
FM, Zaraisky AG, Mishin AS, Dolgov SV, Mitiouchkina TY, Kopantzev EP, Waldenmaier
HE, Oliveira AG, Oba Y, Barsova E, Bogdanova EA, Gabaldón T, Stevani CV, Lukyanov
S, Smirnov IV, Gitelson JI, Kondrashov F, Yampolsky IV. 2018. Genetically encodable
bioluminescent system from fungi. Proceedings of the National Academy of Sciences
of the United States of America. 115(50), 12728–12732.
mla: Kotlobay, Alexey A., et al. “Genetically Encodable Bioluminescent System from
Fungi.” Proceedings of the National Academy of Sciences of the United States
of America, vol. 115, no. 50, National Academy of Sciences, 2018, pp. 12728–32,
doi:10.1073/pnas.1803615115.
short: A.A. Kotlobay, K. Sarkisyan, Y.A. Mokrushina, M. Marcet-Houben, E.O. Serebrovskaya,
N.M. Markina, L. Gonzalez Somermeyer, A.Y. Gorokhovatsky, A. Vvedensky, K.V. Purtov,
V.N. Petushkov, N.S. Rodionova, T.V. Chepurnyh, L. Fakhranurova, E.B. Guglya,
R. Ziganshin, A.S. Tsarkova, Z.M. Kaskova, V. Shender, M. Abakumov, T.O. Abakumova,
I.S. Povolotskaya, F.M. Eroshkin, A.G. Zaraisky, A.S. Mishin, S.V. Dolgov, T.Y.
Mitiouchkina, E.P. Kopantzev, H.E. Waldenmaier, A.G. Oliveira, Y. Oba, E. Barsova,
E.A. Bogdanova, T. Gabaldón, C.V. Stevani, S. Lukyanov, I.V. Smirnov, J.I. Gitelson,
F. Kondrashov, I.V. Yampolsky, Proceedings of the National Academy of Sciences
of the United States of America 115 (2018) 12728–12732.
date_created: 2018-12-23T22:59:18Z
date_published: 2018-12-11T00:00:00Z
date_updated: 2023-09-11T14:04:05Z
day: '11'
ddc:
- '580'
department:
- _id: FyKo
doi: 10.1073/pnas.1803615115
external_id:
isi:
- '000452866000068'
file:
- access_level: open_access
checksum: 46b2c12185eb2ddb598f4c7b4bd267bf
content_type: application/pdf
creator: dernst
date_created: 2019-02-05T15:21:40Z
date_updated: 2020-07-14T12:47:11Z
file_id: '5926'
file_name: 2018_PNAS_Kotlobay.pdf
file_size: 1271988
relation: main_file
file_date_updated: 2020-07-14T12:47:11Z
has_accepted_license: '1'
intvolume: ' 115'
isi: 1
issue: '50'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '12'
oa: 1
oa_version: Published Version
page: 12728-12732
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
issn:
- '00278424'
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genetically encodable bioluminescent system from fungi
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 115
year: '2018'
...
---
_id: '279'
abstract:
- lang: eng
text: 'Background: Natural selection shapes cancer genomes. Previous studies used
signatures of positive selection to identify genes driving malignant transformation.
However, the contribution of negative selection against somatic mutations that
affect essential tumor functions or specific domains remains a controversial topic.
Results: Here, we analyze 7546 individual exomes from 26 tumor types from TCGA
data to explore the portion of the cancer exome under negative selection. Although
we find most of the genes neutrally evolving in a pan-cancer framework, we identify
essential cancer genes and immune-exposed protein regions under significant negative
selection. Moreover, our simulations suggest that the amount of negative selection
is underestimated. We therefore choose an empirical approach to identify genes,
functions, and protein regions under negative selection. We find that expression
and mutation status of negatively selected genes is indicative of patient survival.
Processes that are most strongly conserved are those that play fundamental cellular
roles such as protein synthesis, glucose metabolism, and molecular transport.
Intriguingly, we observe strong signals of selection in the immunopeptidome and
proteins controlling peptide exposition, highlighting the importance of immune
surveillance evasion. Additionally, tumor type-specific immune activity correlates
with the strength of negative selection on human epitopes. Conclusions: In summary,
our results show that negative selection is a hallmark of cell essentiality and
immune response in cancer. The functional domains identified could be exploited
therapeutically, ultimately allowing for the development of novel cancer treatments.'
article_number: '67'
article_processing_charge: No
author:
- first_name: Luis
full_name: Zapata, Luis
last_name: Zapata
- first_name: Oriol
full_name: Pich, Oriol
last_name: Pich
- first_name: Luis
full_name: Serrano, Luis
last_name: Serrano
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Stephan
full_name: Ossowski, Stephan
last_name: Ossowski
- first_name: Martin
full_name: Schaefer, Martin
last_name: Schaefer
citation:
ama: Zapata L, Pich O, Serrano L, Kondrashov F, Ossowski S, Schaefer M. Negative
selection in tumor genome evolution acts on essential cellular functions and the
immunopeptidome. Genome Biology. 2018;19. doi:10.1186/s13059-018-1434-0
apa: Zapata, L., Pich, O., Serrano, L., Kondrashov, F., Ossowski, S., & Schaefer,
M. (2018). Negative selection in tumor genome evolution acts on essential cellular
functions and the immunopeptidome. Genome Biology. BioMed Central. https://doi.org/10.1186/s13059-018-1434-0
chicago: Zapata, Luis, Oriol Pich, Luis Serrano, Fyodor Kondrashov, Stephan Ossowski,
and Martin Schaefer. “Negative Selection in Tumor Genome Evolution Acts on Essential
Cellular Functions and the Immunopeptidome.” Genome Biology. BioMed Central,
2018. https://doi.org/10.1186/s13059-018-1434-0.
ieee: L. Zapata, O. Pich, L. Serrano, F. Kondrashov, S. Ossowski, and M. Schaefer,
“Negative selection in tumor genome evolution acts on essential cellular functions
and the immunopeptidome,” Genome Biology, vol. 19. BioMed Central, 2018.
ista: Zapata L, Pich O, Serrano L, Kondrashov F, Ossowski S, Schaefer M. 2018. Negative
selection in tumor genome evolution acts on essential cellular functions and the
immunopeptidome. Genome Biology. 19, 67.
mla: Zapata, Luis, et al. “Negative Selection in Tumor Genome Evolution Acts on
Essential Cellular Functions and the Immunopeptidome.” Genome Biology,
vol. 19, 67, BioMed Central, 2018, doi:10.1186/s13059-018-1434-0.
short: L. Zapata, O. Pich, L. Serrano, F. Kondrashov, S. Ossowski, M. Schaefer,
Genome Biology 19 (2018).
date_created: 2018-12-11T11:45:35Z
date_published: 2018-05-31T00:00:00Z
date_updated: 2023-09-13T09:01:32Z
day: '31'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1186/s13059-018-1434-0
ec_funded: 1
external_id:
isi:
- '000433986200001'
file:
- access_level: open_access
checksum: f3e4922486bd9bf1483271bdbed394a7
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T14:05:01Z
date_updated: 2020-07-14T12:45:47Z
file_id: '5708'
file_name: 2018_GenomeBiology_Zapata.pdf
file_size: 1414722
relation: main_file
file_date_updated: 2020-07-14T12:45:47Z
has_accepted_license: '1'
intvolume: ' 19'
isi: 1
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
project:
- _id: 26120F5C-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '335980'
name: Systematic investigation of epistasis in molecular evolution
publication: Genome Biology
publication_status: published
publisher: BioMed Central
publist_id: '7620'
quality_controlled: '1'
related_material:
record:
- id: '9811'
relation: research_data
status: public
- id: '9812'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Negative selection in tumor genome evolution acts on essential cellular functions
and the immunopeptidome
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 19
year: '2018'
...
---
_id: '9812'
abstract:
- lang: eng
text: This document contains the full list of genes with their respective significance
and dN/dS values. (TXT 4499Â kb)
article_processing_charge: No
author:
- first_name: Luis
full_name: Zapata, Luis
last_name: Zapata
- first_name: Oriol
full_name: Pich, Oriol
last_name: Pich
- first_name: Luis
full_name: Serrano, Luis
last_name: Serrano
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Stephan
full_name: Ossowski, Stephan
last_name: Ossowski
- first_name: Martin
full_name: Schaefer, Martin
last_name: Schaefer
citation:
ama: 'Zapata L, Pich O, Serrano L, Kondrashov F, Ossowski S, Schaefer M. Additional
file 2: Of negative selection in tumor genome evolution acts on essential cellular
functions and the immunopeptidome. 2018. doi:10.6084/m9.figshare.6401414.v1'
apa: 'Zapata, L., Pich, O., Serrano, L., Kondrashov, F., Ossowski, S., & Schaefer,
M. (2018). Additional file 2: Of negative selection in tumor genome evolution
acts on essential cellular functions and the immunopeptidome. Springer Nature.
https://doi.org/10.6084/m9.figshare.6401414.v1'
chicago: 'Zapata, Luis, Oriol Pich, Luis Serrano, Fyodor Kondrashov, Stephan Ossowski,
and Martin Schaefer. “Additional File 2: Of Negative Selection in Tumor Genome
Evolution Acts on Essential Cellular Functions and the Immunopeptidome.” Springer
Nature, 2018. https://doi.org/10.6084/m9.figshare.6401414.v1.'
ieee: 'L. Zapata, O. Pich, L. Serrano, F. Kondrashov, S. Ossowski, and M. Schaefer,
“Additional file 2: Of negative selection in tumor genome evolution acts on essential
cellular functions and the immunopeptidome.” Springer Nature, 2018.'
ista: 'Zapata L, Pich O, Serrano L, Kondrashov F, Ossowski S, Schaefer M. 2018.
Additional file 2: Of negative selection in tumor genome evolution acts on essential
cellular functions and the immunopeptidome, Springer Nature, 10.6084/m9.figshare.6401414.v1.'
mla: 'Zapata, Luis, et al. Additional File 2: Of Negative Selection in Tumor
Genome Evolution Acts on Essential Cellular Functions and the Immunopeptidome.
Springer Nature, 2018, doi:10.6084/m9.figshare.6401414.v1.'
short: L. Zapata, O. Pich, L. Serrano, F. Kondrashov, S. Ossowski, M. Schaefer,
(2018).
date_created: 2021-08-06T12:58:25Z
date_published: 2018-05-31T00:00:00Z
date_updated: 2023-09-13T09:01:31Z
day: '31'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.6401414.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.6401414.v1
month: '05'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '279'
relation: used_in_publication
status: public
status: public
title: 'Additional file 2: Of negative selection in tumor genome evolution acts on
essential cellular functions and the immunopeptidome'
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2018'
...
---
_id: '9811'
abstract:
- lang: eng
text: This document contains additional supporting evidence presented as supplemental
tables. (XLSX 50Â kb)
article_processing_charge: No
author:
- first_name: Luis
full_name: Zapata, Luis
last_name: Zapata
- first_name: Oriol
full_name: Pich, Oriol
last_name: Pich
- first_name: Luis
full_name: Serrano, Luis
last_name: Serrano
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Stephan
full_name: Ossowski, Stephan
last_name: Ossowski
- first_name: Martin
full_name: Schaefer, Martin
last_name: Schaefer
citation:
ama: 'Zapata L, Pich O, Serrano L, Kondrashov F, Ossowski S, Schaefer M. Additional
file 1: Of negative selection in tumor genome evolution acts on essential cellular
functions and the immunopeptidome. 2018. doi:10.6084/m9.figshare.6401390.v1'
apa: 'Zapata, L., Pich, O., Serrano, L., Kondrashov, F., Ossowski, S., & Schaefer,
M. (2018). Additional file 1: Of negative selection in tumor genome evolution
acts on essential cellular functions and the immunopeptidome. Springer Nature.
https://doi.org/10.6084/m9.figshare.6401390.v1'
chicago: 'Zapata, Luis, Oriol Pich, Luis Serrano, Fyodor Kondrashov, Stephan Ossowski,
and Martin Schaefer. “Additional File 1: Of Negative Selection in Tumor Genome
Evolution Acts on Essential Cellular Functions and the Immunopeptidome.” Springer
Nature, 2018. https://doi.org/10.6084/m9.figshare.6401390.v1.'
ieee: 'L. Zapata, O. Pich, L. Serrano, F. Kondrashov, S. Ossowski, and M. Schaefer,
“Additional file 1: Of negative selection in tumor genome evolution acts on essential
cellular functions and the immunopeptidome.” Springer Nature, 2018.'
ista: 'Zapata L, Pich O, Serrano L, Kondrashov F, Ossowski S, Schaefer M. 2018.
Additional file 1: Of negative selection in tumor genome evolution acts on essential
cellular functions and the immunopeptidome, Springer Nature, 10.6084/m9.figshare.6401390.v1.'
mla: 'Zapata, Luis, et al. Additional File 1: Of Negative Selection in Tumor
Genome Evolution Acts on Essential Cellular Functions and the Immunopeptidome.
Springer Nature, 2018, doi:10.6084/m9.figshare.6401390.v1.'
short: L. Zapata, O. Pich, L. Serrano, F. Kondrashov, S. Ossowski, M. Schaefer,
(2018).
date_created: 2021-08-06T12:53:49Z
date_published: 2018-05-31T00:00:00Z
date_updated: 2023-09-13T09:01:31Z
day: '31'
department:
- _id: FyKo
doi: 10.6084/m9.figshare.6401390.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.6401390.v1
month: '05'
oa: 1
oa_version: Preprint
publisher: Springer Nature
related_material:
record:
- id: '279'
relation: used_in_publication
status: public
status: public
title: 'Additional file 1: Of negative selection in tumor genome evolution acts on
essential cellular functions and the immunopeptidome'
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2018'
...
---
_id: '5995'
abstract:
- lang: eng
text: "Motivation\r\nComputational prediction of the effect of mutations on protein
stability is used by researchers in many fields. The utility of the prediction
methods is affected by their accuracy and bias. Bias, a systematic shift of the
predicted change of stability, has been noted as an issue for several methods,
but has not been investigated systematically. Presence of the bias may lead to
misleading results especially when exploring the effects of combination of different
mutations.\r\n\r\nResults\r\nHere we use a protocol to measure the bias as a function
of the number of introduced mutations. It is based on a self-consistency test
of the reciprocity the effect of a mutation. An advantage of the used approach
is that it relies solely on crystal structures without experimentally measured
stability values. We applied the protocol to four popular algorithms predicting
change of protein stability upon mutation, FoldX, Eris, Rosetta and I-Mutant,
and found an inherent bias. For one program, FoldX, we manage to substantially
reduce the bias using additional relaxation by Modeller. Authors using algorithms
for predicting effects of mutations should be aware of the bias described here."
article_processing_charge: No
author:
- first_name: Dinara R
full_name: Usmanova, Dinara R
last_name: Usmanova
- first_name: Natalya S
full_name: Bogatyreva, Natalya S
last_name: Bogatyreva
- first_name: Joan
full_name: Ariño Bernad, Joan
last_name: Ariño Bernad
- first_name: Aleksandra A
full_name: Eremina, Aleksandra A
last_name: Eremina
- first_name: Anastasiya A
full_name: Gorshkova, Anastasiya A
last_name: Gorshkova
- first_name: German M
full_name: Kanevskiy, German M
last_name: Kanevskiy
- first_name: Lyubov R
full_name: Lonishin, Lyubov R
last_name: Lonishin
- first_name: Alexander V
full_name: Meister, Alexander V
last_name: Meister
- first_name: Alisa G
full_name: Yakupova, Alisa G
last_name: Yakupova
- first_name: Fyodor
full_name: Kondrashov, Fyodor
id: 44FDEF62-F248-11E8-B48F-1D18A9856A87
last_name: Kondrashov
orcid: 0000-0001-8243-4694
- first_name: Dmitry
full_name: Ivankov, Dmitry
id: 49FF1036-F248-11E8-B48F-1D18A9856A87
last_name: Ivankov
citation:
ama: Usmanova DR, Bogatyreva NS, Ariño Bernad J, et al. Self-consistency test reveals
systematic bias in programs for prediction change of stability upon mutation.
Bioinformatics. 2018;34(21):3653-3658. doi:10.1093/bioinformatics/bty340
apa: Usmanova, D. R., Bogatyreva, N. S., Ariño Bernad, J., Eremina, A. A., Gorshkova,
A. A., Kanevskiy, G. M., … Ivankov, D. (2018). Self-consistency test reveals systematic
bias in programs for prediction change of stability upon mutation. Bioinformatics.
Oxford University Press . https://doi.org/10.1093/bioinformatics/bty340
chicago: Usmanova, Dinara R, Natalya S Bogatyreva, Joan Ariño Bernad, Aleksandra
A Eremina, Anastasiya A Gorshkova, German M Kanevskiy, Lyubov R Lonishin, et al.
“Self-Consistency Test Reveals Systematic Bias in Programs for Prediction Change
of Stability upon Mutation.” Bioinformatics. Oxford University Press ,
2018. https://doi.org/10.1093/bioinformatics/bty340.
ieee: D. R. Usmanova et al., “Self-consistency test reveals systematic bias
in programs for prediction change of stability upon mutation,” Bioinformatics,
vol. 34, no. 21. Oxford University Press , pp. 3653–3658, 2018.
ista: Usmanova DR, Bogatyreva NS, Ariño Bernad J, Eremina AA, Gorshkova AA, Kanevskiy
GM, Lonishin LR, Meister AV, Yakupova AG, Kondrashov F, Ivankov D. 2018. Self-consistency
test reveals systematic bias in programs for prediction change of stability upon
mutation. Bioinformatics. 34(21), 3653–3658.
mla: Usmanova, Dinara R., et al. “Self-Consistency Test Reveals Systematic Bias
in Programs for Prediction Change of Stability upon Mutation.” Bioinformatics,
vol. 34, no. 21, Oxford University Press , 2018, pp. 3653–58, doi:10.1093/bioinformatics/bty340.
short: D.R. Usmanova, N.S. Bogatyreva, J. Ariño Bernad, A.A. Eremina, A.A. Gorshkova,
G.M. Kanevskiy, L.R. Lonishin, A.V. Meister, A.G. Yakupova, F. Kondrashov, D.
Ivankov, Bioinformatics 34 (2018) 3653–3658.
date_created: 2019-02-14T12:48:00Z
date_published: 2018-11-01T00:00:00Z
date_updated: 2023-09-19T14:31:13Z
day: '01'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1093/bioinformatics/bty340
ec_funded: 1
external_id:
isi:
- '000450038900008'
pmid:
- '29722803'
file:
- access_level: open_access
checksum: 7e0495153f44211479674601d7f6ee03
content_type: application/pdf
creator: kschuh
date_created: 2019-02-14T13:00:55Z
date_updated: 2020-07-14T12:47:15Z
file_id: '5997'
file_name: 2018_Oxford_Usmanova.pdf
file_size: 291969
relation: main_file
file_date_updated: 2020-07-14T12:47:15Z
has_accepted_license: '1'
intvolume: ' 34'
isi: 1
issue: '21'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 3653-3658
pmid: 1
project:
- _id: 26120F5C-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '335980'
name: Systematic investigation of epistasis in molecular evolution
publication: Bioinformatics
publication_identifier:
issn:
- 1367-4803
- 1460-2059
publication_status: published
publisher: 'Oxford University Press '
quality_controlled: '1'
scopus_import: '1'
status: public
title: Self-consistency test reveals systematic bias in programs for prediction change
of stability upon mutation
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 34
year: '2018'
...
---
_id: '3771'
abstract:
- lang: eng
text: The small-sized frugivorous bat Carollia perspicillata is an understory specialist
and occurs in a wide range of lowland habitats, tending to be more common in tropical
dry or moist forests of South and Central America. Its sister species, Carollia
brevicauda, occurs almost exclusively in the Amazon rainforest. A recent phylogeographic
study proposed a hypothesis of origin and subsequent diversification for C. perspicillata
along the Atlantic coastal forest of Brazil. Additionally, it also found two allopatric
clades for C. brevicauda separated by the Amazon Basin. We used cytochrome b gene
sequences and a more extensive sampling to test hypotheses related to the origin
and diversification of C. perspicillata plus C. brevicauda clade in South America.
The results obtained indicate that there are two sympatric evolutionary lineages
within each species. In C. perspicillata, one lineage is limited to the Southern
Atlantic Forest, whereas the other is widely distributed. Coalescent analysis
points to a simultaneous origin for C. perspicillata and C. brevicauda, although
no place for the diversification of each species can be firmly suggested. The
phylogeographic pattern shown by C. perspicillata is also congruent with the Pleistocene
refugia hypothesis as a likely vicariant phenomenon shaping the present distribution
of its intraspecific lineages.
author:
- first_name: Ana
full_name: Pavan, Ana
last_name: Pavan
- first_name: Felipe
full_name: Martins, Felipe
last_name: Martins
- first_name: Fabrício
full_name: Santos, Fabrício
last_name: Santos
- first_name: Albert
full_name: Ditchfield, Albert
last_name: Ditchfield
- first_name: Rodrigo A
full_name: Fernandes Redondo, Rodrigo A
id: 409D5C96-F248-11E8-B48F-1D18A9856A87
last_name: Fernandes Redondo
orcid: 0000-0002-5837-2793
citation:
ama: 'Pavan A, Martins F, Santos F, Ditchfield A, Fernandes Redondo RA. Patterns
of diversification in two species of short-tailed bats (Carollia Gray, 1838):
the effects of historical fragmentation of Brazilian rainforests. Biological
Journal of the Linnean Society. 2011;102(3):527-539. doi:10.1111/j.1095-8312.2010.01601.x'
apa: 'Pavan, A., Martins, F., Santos, F., Ditchfield, A., & Fernandes Redondo,
R. A. (2011). Patterns of diversification in two species of short-tailed bats
(Carollia Gray, 1838): the effects of historical fragmentation of Brazilian rainforests.
Biological Journal of the Linnean Society. Wiley-Blackwell. https://doi.org/10.1111/j.1095-8312.2010.01601.x'
chicago: 'Pavan, Ana, Felipe Martins, Fabrício Santos, Albert Ditchfield, and Rodrigo
A Fernandes Redondo. “Patterns of Diversification in Two Species of Short-Tailed
Bats (Carollia Gray, 1838): The Effects of Historical Fragmentation of Brazilian
Rainforests.” Biological Journal of the Linnean Society. Wiley-Blackwell,
2011. https://doi.org/10.1111/j.1095-8312.2010.01601.x.'
ieee: 'A. Pavan, F. Martins, F. Santos, A. Ditchfield, and R. A. Fernandes Redondo,
“Patterns of diversification in two species of short-tailed bats (Carollia Gray,
1838): the effects of historical fragmentation of Brazilian rainforests.,” Biological
Journal of the Linnean Society, vol. 102, no. 3. Wiley-Blackwell, pp. 527–539,
2011.'
ista: 'Pavan A, Martins F, Santos F, Ditchfield A, Fernandes Redondo RA. 2011. Patterns
of diversification in two species of short-tailed bats (Carollia Gray, 1838):
the effects of historical fragmentation of Brazilian rainforests. Biological Journal
of the Linnean Society. 102(3), 527–539.'
mla: 'Pavan, Ana, et al. “Patterns of Diversification in Two Species of Short-Tailed
Bats (Carollia Gray, 1838): The Effects of Historical Fragmentation of Brazilian
Rainforests.” Biological Journal of the Linnean Society, vol. 102, no.
3, Wiley-Blackwell, 2011, pp. 527–39, doi:10.1111/j.1095-8312.2010.01601.x.'
short: A. Pavan, F. Martins, F. Santos, A. Ditchfield, R.A. Fernandes Redondo, Biological
Journal of the Linnean Society 102 (2011) 527–539.
date_created: 2018-12-11T12:05:05Z
date_published: 2011-02-10T00:00:00Z
date_updated: 2021-01-12T07:52:05Z
day: '10'
department:
- _id: FyKo
doi: 10.1111/j.1095-8312.2010.01601.x
intvolume: ' 102'
issue: '3'
language:
- iso: eng
month: '02'
oa_version: None
page: 527 - 539
publication: Biological Journal of the Linnean Society
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2456'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Patterns of diversification in two species of short-tailed bats (Carollia
Gray, 1838): the effects of historical fragmentation of Brazilian rainforests.'
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 102
year: '2011'
...