---
_id: '13967'
abstract:
- lang: eng
text: 'A classic solution technique for Markov decision processes (MDP) and stochastic
games (SG) is value iteration (VI). Due to its good practical performance, this
approximative approach is typically preferred over exact techniques, even though
no practical bounds on the imprecision of the result could be given until recently.
As a consequence, even the most used model checkers could return arbitrarily wrong
results. Over the past decade, different works derived stopping criteria, indicating
when the precision reaches the desired level, for various settings, in particular
MDP with reachability, total reward, and mean payoff, and SG with reachability.In
this paper, we provide the first stopping criteria for VI on SG with total reward
and mean payoff, yielding the first anytime algorithms in these settings. To this
end, we provide the solution in two flavours: First through a reduction to the
MDP case and second directly on SG. The former is simpler and automatically utilizes
any advances on MDP. The latter allows for more local computations, heading towards
better practical efficiency.Our solution unifies the previously mentioned approaches
for MDP and SG and their underlying ideas. To achieve this, we isolate objective-specific
subroutines as well as identify objective-independent concepts. These structural
concepts, while surprisingly simple, form the very essence of the unified solution.'
acknowledgement: This research was funded in part by DFG projects 383882557 “SUV”
and 427755713 “GOPro”.
article_processing_charge: No
arxiv: 1
author:
- first_name: Jan
full_name: Kretinsky, Jan
id: 44CEF464-F248-11E8-B48F-1D18A9856A87
last_name: Kretinsky
orcid: 0000-0002-8122-2881
- first_name: Tobias
full_name: Meggendorfer, Tobias
id: b21b0c15-30a2-11eb-80dc-f13ca25802e1
last_name: Meggendorfer
orcid: 0000-0002-1712-2165
- first_name: Maximilian
full_name: Weininger, Maximilian
id: 02ab0197-cc70-11ed-ab61-918e71f56881
last_name: Weininger
orcid: 0000-0002-0163-2152
citation:
ama: 'Kretinsky J, Meggendorfer T, Weininger M. Stopping criteria for value iteration
on stochastic games with quantitative objectives. In: 38th Annual ACM/IEEE
Symposium on Logic in Computer Science. Vol 2023. IEEE; 2023. doi:10.1109/LICS56636.2023.10175771'
apa: 'Kretinsky, J., Meggendorfer, T., & Weininger, M. (2023). Stopping criteria
for value iteration on stochastic games with quantitative objectives. In 38th
Annual ACM/IEEE Symposium on Logic in Computer Science (Vol. 2023). Boston,
MA, United States: IEEE. https://doi.org/10.1109/LICS56636.2023.10175771'
chicago: Kretinsky, Jan, Tobias Meggendorfer, and Maximilian Weininger. “Stopping
Criteria for Value Iteration on Stochastic Games with Quantitative Objectives.”
In 38th Annual ACM/IEEE Symposium on Logic in Computer Science, Vol. 2023.
IEEE, 2023. https://doi.org/10.1109/LICS56636.2023.10175771.
ieee: J. Kretinsky, T. Meggendorfer, and M. Weininger, “Stopping criteria for value
iteration on stochastic games with quantitative objectives,” in 38th Annual
ACM/IEEE Symposium on Logic in Computer Science, Boston, MA, United States,
2023, vol. 2023.
ista: 'Kretinsky J, Meggendorfer T, Weininger M. 2023. Stopping criteria for value
iteration on stochastic games with quantitative objectives. 38th Annual ACM/IEEE
Symposium on Logic in Computer Science. LICS: Logic in Computer Science vol. 2023.'
mla: Kretinsky, Jan, et al. “Stopping Criteria for Value Iteration on Stochastic
Games with Quantitative Objectives.” 38th Annual ACM/IEEE Symposium on Logic
in Computer Science, vol. 2023, IEEE, 2023, doi:10.1109/LICS56636.2023.10175771.
short: J. Kretinsky, T. Meggendorfer, M. Weininger, in:, 38th Annual ACM/IEEE Symposium
on Logic in Computer Science, IEEE, 2023.
conference:
end_date: 2023-06-29
location: Boston, MA, United States
name: 'LICS: Logic in Computer Science'
start_date: 2023-06-26
corr_author: '1'
date_created: 2023-08-06T22:01:10Z
date_published: 2023-07-01T00:00:00Z
date_updated: 2026-08-12T06:39:58Z
day: '01'
department:
- _id: KrCh
doi: 10.1109/LICS56636.2023.10175771
external_id:
arxiv:
- '2304.09930'
isi:
- '001036707700042'
intvolume: ' 2023'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.48550/arXiv.2304.09930
month: '07'
oa: 1
oa_version: Preprint
publication: 38th Annual ACM/IEEE Symposium on Logic in Computer Science
publication_identifier:
isbn:
- '9798350335873'
issn:
- 1043-6871
publication_status: published
publisher: IEEE
quality_controlled: '1'
scopus_import: '1'
status: public
title: Stopping criteria for value iteration on stochastic games with quantitative
objectives
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2023
year: '2023'
...
---
_id: '14751'
abstract:
- lang: eng
text: 'We consider zero-error communication over a two-transmitter deterministic
adversarial multiple access channel (MAC) governed by an adversary who has access
to the transmissions of both senders (hence called omniscient ) and aims to maliciously
corrupt the communication. None of the encoders, jammer and decoder is allowed
to randomize using private or public randomness. This enforces a combinatorial
nature of the problem. Our model covers a large family of channels studied in
the literature, including all deterministic discrete memoryless noisy or noiseless
MACs. In this work, given an arbitrary two-transmitter deterministic omniscient
adversarial MAC, we characterize when the capacity region: 1) has nonempty interior
(in particular, is two-dimensional); 2) consists of two line segments (in particular,
has empty interior); 3) consists of one line segment (in particular, is one-dimensional);
4) or only contains (0,0) (in particular, is zero-dimensional). This extends a
recent result by Wang et al. (201 9) from the point-to-point setting to the multiple
access setting. Indeed, our converse arguments build upon their generalized Plotkin
bound and involve delicate case analysis. One of the technical challenges is to
take care of both “joint confusability” and “marginal confusability”. In particular,
the treatment of marginal confusability does not follow from the point-to-point
results by Wang et al. Our achievability results follow from random coding with
expurgation.'
acknowledgement: "The author would like to thank Amitalok J. Budkuley and Sidharth
Jaggi for many helpful discussions at the early stage of this work. He would also
like to thank Nir Ailon, Qi Cao, and Chandra Nair for discussions on a related problem
regarding zero-error binary adder MACs.\r\nThe work of Yihan Zhang was supported
by the European Union’s Horizon 2020 Research and Innovation Programme under Grant
682203-ERC-[Inf-Speed-Tradeoff]"
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Yihan
full_name: Zhang, Yihan
id: 2ce5da42-b2ea-11eb-bba5-9f264e9d002c
last_name: Zhang
orcid: 0000-0002-6465-6258
citation:
ama: Zhang Y. Zero-error communication over adversarial MACs. IEEE Transactions
on Information Theory. 2023;69(7):4093-4127. doi:10.1109/tit.2023.3257239
apa: Zhang, Y. (2023). Zero-error communication over adversarial MACs. IEEE Transactions
on Information Theory. IEEE. https://doi.org/10.1109/tit.2023.3257239
chicago: Zhang, Yihan. “Zero-Error Communication over Adversarial MACs.” IEEE
Transactions on Information Theory. IEEE, 2023. https://doi.org/10.1109/tit.2023.3257239.
ieee: Y. Zhang, “Zero-error communication over adversarial MACs,” IEEE Transactions
on Information Theory, vol. 69, no. 7. IEEE, pp. 4093–4127, 2023.
ista: Zhang Y. 2023. Zero-error communication over adversarial MACs. IEEE Transactions
on Information Theory. 69(7), 4093–4127.
mla: Zhang, Yihan. “Zero-Error Communication over Adversarial MACs.” IEEE Transactions
on Information Theory, vol. 69, no. 7, IEEE, 2023, pp. 4093–127, doi:10.1109/tit.2023.3257239.
short: Y. Zhang, IEEE Transactions on Information Theory 69 (2023) 4093–4127.
corr_author: '1'
date_created: 2024-01-08T13:04:54Z
date_published: 2023-07-01T00:00:00Z
date_updated: 2026-08-12T06:41:37Z
day: '01'
department:
- _id: MaMo
doi: 10.1109/tit.2023.3257239
external_id:
arxiv:
- '2101.12426'
isi:
- '001017307000001'
intvolume: ' 69'
isi: 1
issue: '7'
keyword:
- Computer Science Applications
- Information Systems
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.48550/arXiv.2101.12426
month: '07'
oa: 1
oa_version: Preprint
page: 4093-4127
publication: IEEE Transactions on Information Theory
publication_identifier:
eissn:
- 1557-9654
issn:
- 0018-9448
publication_status: published
publisher: IEEE
quality_controlled: '1'
scopus_import: '1'
status: public
title: Zero-error communication over adversarial MACs
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 69
year: '2023'
...
---
_id: '13269'
abstract:
- lang: eng
text: This paper is a collection of results on combinatorial properties of codes
for the Z-channel . A Z-channel with error fraction τ takes as input a length-
n binary codeword and injects in an adversarial manner up to n τ asymmetric errors,
i.e., errors that only zero out bits but do not flip 0’s to 1’s. It is known that
the largest ( L - 1)-list-decodable code for the Z-channel with error fraction
τ has exponential size (in n ) if τ is less than a critical value that we call
the ( L - 1)- list-decoding Plotkin point and has constant size if τ is larger
than the threshold. The ( L -1)-list-decoding Plotkin point is known to be L -1/L-1
– L -L/ L-1 , which equals 1/4 for unique-decoding with L -1 = 1. In this paper,
we derive various results for the size of the largest codes above and below the
list-decoding Plotkin point. In particular, we show that the largest ( L -1)-list-decodable
code ε-above the Plotkin point, for any given sufficiently small positive constant
ε > 0, has size Θ L (ε -3/2 ) for any L - 1 ≥ 1. We also devise upper and lower
bounds on the exponential size of codes below the list-decoding Plotkin point.
acknowledgement: "Nikita Polyanskii’s research was conducted in part during October
2020 - December 2021 with the Technical University of Munich and the Skolkovo Institute
of Science and Technology. His work was supported by the German Research Foundation
(Deutsche Forschungsgemeinschaft, DFG) under Grant No. WA3907/1-1 and the Russian
Foundation for Basic Research (RFBR)\r\nunder Grant No. 20-01-00559.\r\nYihan Zhang
is supported by funding from the European Union’s Horizon 2020 research and innovation
programme under grant agreement No 682203-ERC-[Inf-Speed-Tradeoff]."
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Nikita
full_name: Polyanskii, Nikita
last_name: Polyanskii
- first_name: Yihan
full_name: Zhang, Yihan
id: 2ce5da42-b2ea-11eb-bba5-9f264e9d002c
last_name: Zhang
orcid: 0000-0002-6465-6258
citation:
ama: Polyanskii N, Zhang Y. Codes for the Z-channel. IEEE Transactions on Information
Theory. 2023;69(10):6340-6357. doi:10.1109/TIT.2023.3292219
apa: Polyanskii, N., & Zhang, Y. (2023). Codes for the Z-channel. IEEE Transactions
on Information Theory. IEEE. https://doi.org/10.1109/TIT.2023.3292219
chicago: Polyanskii, Nikita, and Yihan Zhang. “Codes for the Z-Channel.” IEEE
Transactions on Information Theory. IEEE, 2023. https://doi.org/10.1109/TIT.2023.3292219.
ieee: N. Polyanskii and Y. Zhang, “Codes for the Z-channel,” IEEE Transactions
on Information Theory, vol. 69, no. 10. IEEE, pp. 6340–6357, 2023.
ista: Polyanskii N, Zhang Y. 2023. Codes for the Z-channel. IEEE Transactions on
Information Theory. 69(10), 6340–6357.
mla: Polyanskii, Nikita, and Yihan Zhang. “Codes for the Z-Channel.” IEEE Transactions
on Information Theory, vol. 69, no. 10, IEEE, 2023, pp. 6340–57, doi:10.1109/TIT.2023.3292219.
short: N. Polyanskii, Y. Zhang, IEEE Transactions on Information Theory 69 (2023)
6340–6357.
corr_author: '1'
date_created: 2023-07-23T22:01:14Z
date_published: 2023-07-04T00:00:00Z
date_updated: 2026-08-12T06:41:54Z
day: '04'
department:
- _id: MaMo
doi: 10.1109/TIT.2023.3292219
external_id:
arxiv:
- '2105.01427'
isi:
- '001069680100011'
intvolume: ' 69'
isi: 1
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.48550/arXiv.2105.01427
month: '07'
oa: 1
oa_version: Preprint
page: 6340-6357
publication: IEEE Transactions on Information Theory
publication_identifier:
eissn:
- 1557-9654
issn:
- 0018-9448
publication_status: published
publisher: IEEE
quality_controlled: '1'
scopus_import: '1'
status: public
title: Codes for the Z-channel
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 69
year: '2023'
...
---
_id: '13221'
abstract:
- lang: eng
text: The safety-liveness dichotomy is a fundamental concept in formal languages
which plays a key role in verification. Recently, this dichotomy has been lifted
to quantitative properties, which are arbitrary functions from infinite words
to partially-ordered domains. We look into harnessing the dichotomy for the specific
classes of quantitative properties expressed by quantitative automata. These automata
contain finitely many states and rational-valued transition weights, and their
common value functions Inf, Sup, LimInf, LimSup, LimInfAvg, LimSupAvg, and DSum
map infinite words into the totallyordered domain of real numbers. In this automata-theoretic
setting, we establish a connection between quantitative safety and topological
continuity and provide an alternative characterization of quantitative safety
and liveness in terms of their boolean counterparts. For all common value functions,
we show how the safety closure of a quantitative automaton can be constructed
in PTime, and we provide PSpace-complete checks of whether a given quantitative
automaton is safe or live, with the exception of LimInfAvg and LimSupAvg automata,
for which the safety check is in ExpSpace. Moreover, for deterministic Sup, LimInf,
and LimSup automata, we give PTime decompositions into safe and live automata.
These decompositions enable the separation of techniques for safety and liveness
verification for quantitative specifications.
acknowledgement: We thank Christof Löding for pointing us to some results on PSpace-hardess
of universality problems and the anonymous reviewers for their helpful comments.
This work was supported in part by the ERC-2020-AdG 101020093 and the Israel Science
Foundation grant 2410/22.
alternative_title:
- LIPIcs
article_number: '17'
article_processing_charge: No
arxiv: 1
author:
- first_name: Udi
full_name: Boker, Udi
id: 31E297B6-F248-11E8-B48F-1D18A9856A87
last_name: Boker
- first_name: Thomas A
full_name: Henzinger, Thomas A
id: 40876CD8-F248-11E8-B48F-1D18A9856A87
last_name: Henzinger
orcid: 0000-0002-2985-7724
- first_name: Nicolas Adrien
full_name: Mazzocchi, Nicolas Adrien
id: b26baa86-3308-11ec-87b0-8990f34baa85
last_name: Mazzocchi
- first_name: Naci E
full_name: Sarac, Naci E
id: 8C6B42F8-C8E6-11E9-A03A-F2DCE5697425
last_name: Sarac
citation:
ama: 'Boker U, Henzinger TA, Mazzocchi NA, Sarac NE. Safety and liveness of quantitative
automata. In: 34th International Conference on Concurrency Theory. Vol
279. Schloss Dagstuhl - Leibniz-Zentrum für Informatik; 2023. doi:10.4230/LIPIcs.CONCUR.2023.17'
apa: 'Boker, U., Henzinger, T. A., Mazzocchi, N. A., & Sarac, N. E. (2023).
Safety and liveness of quantitative automata. In 34th International Conference
on Concurrency Theory (Vol. 279). Antwerp, Belgium: Schloss Dagstuhl - Leibniz-Zentrum
für Informatik. https://doi.org/10.4230/LIPIcs.CONCUR.2023.17'
chicago: Boker, Udi, Thomas A Henzinger, Nicolas Adrien Mazzocchi, and Naci E Sarac.
“Safety and Liveness of Quantitative Automata.” In 34th International Conference
on Concurrency Theory, Vol. 279. Schloss Dagstuhl - Leibniz-Zentrum für Informatik,
2023. https://doi.org/10.4230/LIPIcs.CONCUR.2023.17.
ieee: U. Boker, T. A. Henzinger, N. A. Mazzocchi, and N. E. Sarac, “Safety and liveness
of quantitative automata,” in 34th International Conference on Concurrency
Theory, Antwerp, Belgium, 2023, vol. 279.
ista: 'Boker U, Henzinger TA, Mazzocchi NA, Sarac NE. 2023. Safety and liveness
of quantitative automata. 34th International Conference on Concurrency Theory.
CONCUR: Conference on Concurrency Theory, LIPIcs, vol. 279, 17.'
mla: Boker, Udi, et al. “Safety and Liveness of Quantitative Automata.” 34th
International Conference on Concurrency Theory, vol. 279, 17, Schloss Dagstuhl
- Leibniz-Zentrum für Informatik, 2023, doi:10.4230/LIPIcs.CONCUR.2023.17.
short: U. Boker, T.A. Henzinger, N.A. Mazzocchi, N.E. Sarac, in:, 34th International
Conference on Concurrency Theory, Schloss Dagstuhl - Leibniz-Zentrum für Informatik,
2023.
conference:
end_date: 2023-09-23
location: Antwerp, Belgium
name: 'CONCUR: Conference on Concurrency Theory'
start_date: 2023-09-18
corr_author: '1'
date_created: 2023-07-14T10:00:15Z
date_published: 2023-09-01T00:00:00Z
date_updated: 2026-08-12T08:46:04Z
day: '01'
ddc:
- '000'
department:
- _id: GradSch
- _id: ToHe
doi: 10.4230/LIPIcs.CONCUR.2023.17
ec_funded: 1
external_id:
arxiv:
- '2307.06016'
isi:
- '001570542500017'
file:
- access_level: open_access
checksum: d40e57a04448ea5c77d7e1cfb9590a81
content_type: application/pdf
creator: esarac
date_created: 2023-07-14T12:03:48Z
date_updated: 2023-07-14T12:03:48Z
file_id: '13224'
file_name: CONCUR23.pdf
file_size: 755529
relation: main_file
success: 1
file_date_updated: 2023-07-14T12:03:48Z
has_accepted_license: '1'
intvolume: ' 279'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 62781420-2b32-11ec-9570-8d9b63373d4d
call_identifier: H2020
grant_number: '101020093'
name: Vigilant Algorithmic Monitoring of Software
publication: 34th International Conference on Concurrency Theory
publication_identifier:
eissn:
- 1868-8969
isbn:
- '9783959772990'
publication_status: published
publisher: Schloss Dagstuhl - Leibniz-Zentrum für Informatik
quality_controlled: '1'
related_material:
record:
- id: '20147'
relation: dissertation_contains
status: public
- id: '20342'
relation: later_version
status: public
scopus_import: '1'
status: public
title: Safety and liveness of quantitative automata
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 279
year: '2023'
...
---
_id: '13965'
abstract:
- lang: eng
text: Many modes and mechanisms of epigenetic inheritance have been elucidated in
eukaryotes. Most of them are relatively short-term, generally not exceeding one
or a few organismal generations. However, emerging evidence indicates that one
mechanism, cytosine DNA methylation, can mediate epigenetic inheritance over much
longer timescales, which are mostly or completely inaccessible in the laboratory.
Here we discuss the evidence for, and mechanisms and implications of, such long-term
epigenetic inheritance. We argue that compelling evidence supports the long-term
epigenetic inheritance of gene body methylation, at least in the model angiosperm
Arabidopsis thaliana, and that variation in such methylation can therefore serve
as an epigenetic basis for phenotypic variation in natural populations.
article_number: '102087'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Elizabeth
full_name: Hollwey, Elizabeth
id: b8c4f54b-e484-11eb-8fdc-a54df64ef6dd
last_name: Hollwey
- first_name: Amy
full_name: Briffa, Amy
last_name: Briffa
- first_name: Martin
full_name: Howard, Martin
last_name: Howard
- first_name: Daniel
full_name: Zilberman, Daniel
id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1
last_name: Zilberman
orcid: 0000-0002-0123-8649
citation:
ama: Hollwey E, Briffa A, Howard M, Zilberman D. Concepts, mechanisms and implications
of long-term epigenetic inheritance. Current Opinion in Genetics & Development.
2023;81(8). doi:10.1016/j.gde.2023.102087
apa: Hollwey, E., Briffa, A., Howard, M., & Zilberman, D. (2023). Concepts,
mechanisms and implications of long-term epigenetic inheritance. Current Opinion
in Genetics & Development. Elsevier. https://doi.org/10.1016/j.gde.2023.102087
chicago: Hollwey, Elizabeth, Amy Briffa, Martin Howard, and Daniel Zilberman. “Concepts,
Mechanisms and Implications of Long-Term Epigenetic Inheritance.” Current Opinion
in Genetics & Development. Elsevier, 2023. https://doi.org/10.1016/j.gde.2023.102087.
ieee: E. Hollwey, A. Briffa, M. Howard, and D. Zilberman, “Concepts, mechanisms
and implications of long-term epigenetic inheritance,” Current Opinion in Genetics
& Development, vol. 81, no. 8. Elsevier, 2023.
ista: Hollwey E, Briffa A, Howard M, Zilberman D. 2023. Concepts, mechanisms and
implications of long-term epigenetic inheritance. Current Opinion in Genetics
& Development. 81(8), 102087.
mla: Hollwey, Elizabeth, et al. “Concepts, Mechanisms and Implications of Long-Term
Epigenetic Inheritance.” Current Opinion in Genetics & Development,
vol. 81, no. 8, 102087, Elsevier, 2023, doi:10.1016/j.gde.2023.102087.
short: E. Hollwey, A. Briffa, M. Howard, D. Zilberman, Current Opinion in Genetics
& Development 81 (2023).
corr_author: '1'
date_created: 2023-08-06T22:01:10Z
date_published: 2023-08-01T00:00:00Z
date_updated: 2026-08-12T09:55:32Z
day: '01'
ddc:
- '570'
department:
- _id: DaZi
doi: 10.1016/j.gde.2023.102087
external_id:
isi:
- '001047020200001'
pmid:
- '37441873'
file:
- access_level: open_access
checksum: a294cd9506b80ed6ef218ef44ed32765
content_type: application/pdf
creator: dernst
date_created: 2023-08-07T08:32:26Z
date_updated: 2023-08-07T08:32:26Z
file_id: '13980'
file_name: 2023_CurrentOpinionGenetics_Hollwey.pdf
file_size: 2568632
relation: main_file
success: 1
file_date_updated: 2023-08-07T08:32:26Z
has_accepted_license: '1'
intvolume: ' 81'
isi: 1
issue: '8'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: Current Opinion in Genetics & Development
publication_identifier:
eissn:
- 1879-0380
issn:
- 0959-437X
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Concepts, mechanisms and implications of long-term epigenetic inheritance
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 81
year: '2023'
...
---
_id: '14774'
abstract:
- lang: eng
text: Morphogen gradients impart positional information to cells in a homogenous
tissue field. Fgf8a, a highly conserved growth factor, has been proposed to act
as a morphogen during zebrafish gastrulation. However, technical limitations have
so far prevented direct visualization of the endogenous Fgf8a gradient and confirmation
of its morphogenic activity. Here, we monitor Fgf8a propagation in the developing
neural plate using a CRISPR/Cas9-mediated EGFP knock-in at the endogenous fgf8a
locus. By combining sensitive imaging with single-molecule fluorescence correlation
spectroscopy, we demonstrate that Fgf8a, which is produced at the embryonic margin,
propagates by diffusion through the extracellular space and forms a graded distribution
towards the animal pole. Overlaying the Fgf8a gradient curve with expression profiles
of its downstream targets determines the precise input-output relationship of
Fgf8a-mediated patterning. Manipulation of the extracellular Fgf8a levels alters
the signaling outcome, thus establishing Fgf8a as a bona fide morphogen during
zebrafish gastrulation. Furthermore, by hindering Fgf8a diffusion, we demonstrate
that extracellular diffusion of the protein from the source is crucial for it
to achieve its morphogenic potential.
acknowledgement: "We thank members of the Brand lab, as well as Justina Stark (Ivo
Sbalzarini group, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden,
Germany) for project-related discussions; Darren Gilmour (University of Zurich),
Karuna Sampath (University of Warwick) and Gokul Kesavan (Vowels Lifesciences Private
Limited, Bangalore) for comments on the manuscript; personnel of the CMCB technology
platform, TU Dresden for imaging and image analysis-related support; and Maurizio
Abbate (Technical support, Arivis) for help with image analysis. We are also grateful
to Stapornwongkul and Briscoe for commenting on a preprint version of our work (Stapornwongkul
and Briscoe, 2022).\r\nThis work was supported by the Deutsche Forschungsgemeinschaft
(BR 1746/6-2, BR 1746/11-1 and BR 1746/3 to M.B.), by a Cluster of Excellence ‘Physics
of Life’ seed grant and by institutional funds from Technische Universitat Dresden
(to M.B.). Open Access funding provided by Technische Universitat Dresden. Deposited
in PMC for immediate release."
article_number: dev201559
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Rohit K
full_name: Harish, Rohit K
id: 1bae78aa-ee0e-11ec-9b76-bc42990f409d
last_name: Harish
- first_name: Mansi
full_name: Gupta, Mansi
last_name: Gupta
- first_name: Daniela
full_name: Zöller, Daniela
last_name: Zöller
- first_name: Hella
full_name: Hartmann, Hella
last_name: Hartmann
- first_name: Ali
full_name: Gheisari, Ali
last_name: Gheisari
- first_name: Anja
full_name: Machate, Anja
last_name: Machate
- first_name: Stefan
full_name: Hans, Stefan
last_name: Hans
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
citation:
ama: Harish RK, Gupta M, Zöller D, et al. Real-time monitoring of an endogenous
Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation.
Development. 2023;150(19). doi:10.1242/dev.201559
apa: Harish, R. K., Gupta, M., Zöller, D., Hartmann, H., Gheisari, A., Machate,
A., … Brand, M. (2023). Real-time monitoring of an endogenous Fgf8a gradient attests
to its role as a morphogen during zebrafish gastrulation. Development.
Company of Biologists. https://doi.org/10.1242/dev.201559
chicago: Harish, Rohit K, Mansi Gupta, Daniela Zöller, Hella Hartmann, Ali Gheisari,
Anja Machate, Stefan Hans, and Michael Brand. “Real-Time Monitoring of an Endogenous
Fgf8a Gradient Attests to Its Role as a Morphogen during Zebrafish Gastrulation.”
Development. Company of Biologists, 2023. https://doi.org/10.1242/dev.201559.
ieee: R. K. Harish et al., “Real-time monitoring of an endogenous Fgf8a gradient
attests to its role as a morphogen during zebrafish gastrulation,” Development,
vol. 150, no. 19. Company of Biologists, 2023.
ista: Harish RK, Gupta M, Zöller D, Hartmann H, Gheisari A, Machate A, Hans S, Brand
M. 2023. Real-time monitoring of an endogenous Fgf8a gradient attests to its role
as a morphogen during zebrafish gastrulation. Development. 150(19), dev201559.
mla: Harish, Rohit K., et al. “Real-Time Monitoring of an Endogenous Fgf8a Gradient
Attests to Its Role as a Morphogen during Zebrafish Gastrulation.” Development,
vol. 150, no. 19, dev201559, Company of Biologists, 2023, doi:10.1242/dev.201559.
short: R.K. Harish, M. Gupta, D. Zöller, H. Hartmann, A. Gheisari, A. Machate, S.
Hans, M. Brand, Development 150 (2023).
date_created: 2024-01-10T09:18:54Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2026-08-12T09:59:59Z
day: '01'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1242/dev.201559
external_id:
isi:
- '001097449100002'
pmid:
- '37665167'
file:
- access_level: open_access
checksum: 2d6f52dc33260a9b2352b8f28374ba5f
content_type: application/pdf
creator: dernst
date_created: 2024-01-10T12:41:13Z
date_updated: 2024-01-10T12:41:13Z
file_id: '14790'
file_name: 2023_Development_Harish.pdf
file_size: 12836306
relation: main_file
success: 1
file_date_updated: 2024-01-10T12:41:13Z
has_accepted_license: '1'
intvolume: ' 150'
isi: 1
issue: '19'
keyword:
- Developmental Biology
- Molecular Biology
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
eissn:
- 1477-9129
issn:
- 0950-1991
publication_status: published
publisher: Company of Biologists
quality_controlled: '1'
status: public
title: Real-time monitoring of an endogenous Fgf8a gradient attests to its role as
a morphogen during zebrafish gastrulation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 150
year: '2023'
...
---
_id: '14657'
abstract:
- lang: eng
text: 'Natural selection is usually studied between mutants that differ in reproductive
rate, but are subject to the same population structure. Here we explore how natural
selection acts on mutants that have the same reproductive rate, but different
population structures. In our framework, population structure is given by a graph
that specifies where offspring can disperse. The invading mutant disperses offspring
on a different graph than the resident wild-type. We find that more densely connected
dispersal graphs tend to increase the invader’s fixation probability, but the
exact relationship between structure and fixation probability is subtle. We present
three main results. First, we prove that if both invader and resident are on complete
dispersal graphs, then removing a single edge in the invader’s dispersal graph
reduces its fixation probability. Second, we show that for certain island models
higher invader’s connectivity increases its fixation probability, but the magnitude
of the effect depends on the exact layout of the connections. Third, we show that
for lattices the effect of different connectivity is comparable to that of different
fitness: for large population size, the invader’s fixation probability is either
constant or exponentially small, depending on whether it is more or less connected
than the resident.'
acknowledgement: K.C. acknowledges support from the ERC CoG 863818(ForM-SMArt). J.T.
is supported by Center for Foundations ofModern Computer Science (Charles Univ.
project UNCE/SCI/004).
article_number: '20230355'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Josef
full_name: Tkadlec, Josef
id: 3F24CCC8-F248-11E8-B48F-1D18A9856A87
last_name: Tkadlec
orcid: 0000-0002-1097-9684
- first_name: Kamran
full_name: Kaveh, Kamran
last_name: Kaveh
- first_name: Krishnendu
full_name: Chatterjee, Krishnendu
id: 2E5DCA20-F248-11E8-B48F-1D18A9856A87
last_name: Chatterjee
orcid: 0000-0002-4561-241X
- first_name: Martin A.
full_name: Nowak, Martin A.
last_name: Nowak
citation:
ama: Tkadlec J, Kaveh K, Chatterjee K, Nowak MA. Evolutionary dynamics of mutants
that modify population structure. Journal of the Royal Society Interface.
2023;20(208). doi:10.1098/rsif.2023.0355
apa: Tkadlec, J., Kaveh, K., Chatterjee, K., & Nowak, M. A. (2023). Evolutionary
dynamics of mutants that modify population structure. Journal of the Royal
Society Interface. Royal Society. https://doi.org/10.1098/rsif.2023.0355
chicago: Tkadlec, Josef, Kamran Kaveh, Krishnendu Chatterjee, and Martin A. Nowak.
“Evolutionary Dynamics of Mutants That Modify Population Structure.” Journal
of the Royal Society Interface. Royal Society, 2023. https://doi.org/10.1098/rsif.2023.0355.
ieee: J. Tkadlec, K. Kaveh, K. Chatterjee, and M. A. Nowak, “Evolutionary dynamics
of mutants that modify population structure,” Journal of the Royal Society
Interface, vol. 20, no. 208. Royal Society, 2023.
ista: Tkadlec J, Kaveh K, Chatterjee K, Nowak MA. 2023. Evolutionary dynamics of
mutants that modify population structure. Journal of the Royal Society Interface.
20(208), 20230355.
mla: Tkadlec, Josef, et al. “Evolutionary Dynamics of Mutants That Modify Population
Structure.” Journal of the Royal Society Interface, vol. 20, no. 208, 20230355,
Royal Society, 2023, doi:10.1098/rsif.2023.0355.
short: J. Tkadlec, K. Kaveh, K. Chatterjee, M.A. Nowak, Journal of the Royal Society
Interface 20 (2023).
date_created: 2023-12-10T23:00:58Z
date_published: 2023-11-29T00:00:00Z
date_updated: 2026-08-12T14:05:25Z
day: '29'
ddc:
- '000'
- '570'
department:
- _id: KrCh
doi: 10.1098/rsif.2023.0355
ec_funded: 1
external_id:
isi:
- '001124419300002'
pmid:
- '38016637'
file:
- access_level: open_access
checksum: 2eefab13127c7786dbd33303c482a004
content_type: application/pdf
creator: dernst
date_created: 2023-12-11T11:10:32Z
date_updated: 2023-12-11T11:10:32Z
file_id: '14673'
file_name: 2023_RoyalInterface_Tkadlec.pdf
file_size: 1720243
relation: main_file
success: 1
file_date_updated: 2023-12-11T11:10:32Z
has_accepted_license: '1'
intvolume: ' 20'
isi: 1
issue: '208'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 0599E47C-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '863818'
name: 'Formal Methods for Stochastic Models: Algorithms and Applications'
publication: Journal of the Royal Society Interface
publication_identifier:
eissn:
- 1742-5662
publication_status: published
publisher: Royal Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Evolutionary dynamics of mutants that modify population structure
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 20
year: '2023'
...
---
OA_place: publisher
OA_type: gold
_id: '14953'
abstract:
- lang: eng
text: "This paper provides statistical sample complexity bounds for score-matching
and\r\nits applications in causal discovery. We demonstrate that accurate estimation
of the\r\nscore function is achievable by training a standard deep ReLU neural
network using\r\nstochastic gradient descent. We establish bounds on the error
rate of recovering\r\ncausal relationships using the score-matching-based causal
discovery method of\r\nRolland et al. [2022], assuming a sufficiently good estimation
of the score function.\r\nFinally, we analyze the upper bound of score-matching
estimation within the scorebased generative modeling, which has been applied for
causal discovery but is also\r\nof independent interest within the domain of generative
models.y"
acknowledgement: "We are thankful to the reviewers for providing constructive feedback
and Kun Zhang and Dominik\r\nJanzing for helpful discussion on the special case
of deterministic children. This work was supported\r\nby Hasler Foundation Program:
Hasler Responsible AI (project number 21043). This work was\r\nsupported by the
Swiss National Science Foundation (SNSF) under grant number 200021_205011.\r\nFrancesco
Locatello did not contribute to this work at Amazon. "
alternative_title:
- Advances in Neural Information Processing Systems
article_processing_charge: No
arxiv: 1
author:
- first_name: Zhenyu
full_name: Zhu, Zhenyu
last_name: Zhu
- first_name: Francesco
full_name: Locatello, Francesco
id: 26cfd52f-2483-11ee-8040-88983bcc06d4
last_name: Locatello
orcid: 0000-0002-4850-0683
- first_name: Volkan
full_name: Cevher, Volkan
last_name: Cevher
citation:
ama: 'Zhu Z, Locatello F, Cevher V. Sample complexity bounds for score-matching:
Causal discovery and generative modeling. In: 37th Conference on Neural Information
Processing Systems. Vol 36. Neural Information Processing Systems Foundation;
2023:3325-3337. doi:10.52202/075280-0147'
apa: 'Zhu, Z., Locatello, F., & Cevher, V. (2023). Sample complexity bounds
for score-matching: Causal discovery and generative modeling. In 37th Conference
on Neural Information Processing Systems (Vol. 36, pp. 3325–3337). New Orleans,
LO, United States: Neural Information Processing Systems Foundation. https://doi.org/10.52202/075280-0147'
chicago: 'Zhu, Zhenyu, Francesco Locatello, and Volkan Cevher. “Sample Complexity
Bounds for Score-Matching: Causal Discovery and Generative Modeling.” In 37th
Conference on Neural Information Processing Systems, 36:3325–37. Neural Information
Processing Systems Foundation, 2023. https://doi.org/10.52202/075280-0147.'
ieee: 'Z. Zhu, F. Locatello, and V. Cevher, “Sample complexity bounds for score-matching:
Causal discovery and generative modeling,” in 37th Conference on Neural Information
Processing Systems, New Orleans, LO, United States, 2023, vol. 36, pp. 3325–3337.'
ista: 'Zhu Z, Locatello F, Cevher V. 2023. Sample complexity bounds for score-matching:
Causal discovery and generative modeling. 37th Conference on Neural Information
Processing Systems. NeurIPS: Neural Information Processing Systems, Advances in
Neural Information Processing Systems, vol. 36, 3325–3337.'
mla: 'Zhu, Zhenyu, et al. “Sample Complexity Bounds for Score-Matching: Causal Discovery
and Generative Modeling.” 37th Conference on Neural Information Processing
Systems, vol. 36, Neural Information Processing Systems Foundation, 2023,
pp. 3325–37, doi:10.52202/075280-0147.'
short: Z. Zhu, F. Locatello, V. Cevher, in:, 37th Conference on Neural Information
Processing Systems, Neural Information Processing Systems Foundation, 2023, pp.
3325–3337.
conference:
end_date: 2023-12-16
location: New Orleans, LO, United States
name: 'NeurIPS: Neural Information Processing Systems'
start_date: 2023-12-12
corr_author: '1'
das_tickbox: '0'
date_created: 2024-02-07T15:11:11Z
date_published: 2023-10-27T00:00:00Z
date_updated: 2026-08-13T07:09:06Z
day: '27'
ddc:
- '000'
department:
- _id: FrLo
doi: 10.52202/075280-0147
external_id:
arxiv:
- '2310.18123'
file:
- access_level: open_access
checksum: 6f10adadefceb3bb50ad0b2637493381
content_type: application/pdf
creator: dernst
date_created: 2026-08-13T07:02:51Z
date_updated: 2026-08-13T07:02:51Z
file_id: '22700'
file_name: 2023_Neurips_Zhu.pdf
file_size: 305362
relation: main_file
success: 1
file_date_updated: 2026-08-13T07:02:51Z
has_accepted_license: '1'
intvolume: ' 36'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 3325-3337
publication: 37th Conference on Neural Information Processing Systems
publication_identifier:
eissn:
- 1049-5258
isbn:
- '9781713899921'
publication_status: published
publisher: Neural Information Processing Systems Foundation
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: yes
title: 'Sample complexity bounds for score-matching: Causal discovery and generative
modeling'
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2023'
...
---
OA_place: publisher
OA_type: gold
_id: '14954'
abstract:
- lang: eng
text: "When domain knowledge is limited and experimentation is restricted by ethical,\r\nfinancial,
or time constraints, practitioners turn to observational causal discovery\r\nmethods
to recover the causal structure, exploiting the statistical properties of their\r\ndata.
Because causal discovery without further assumptions is an ill-posed problem,\r\neach
algorithm comes with its own set of usually untestable assumptions, some\r\nof
which are hard to meet in real datasets. Motivated by these considerations, this\r\npaper
extensively benchmarks the empirical performance of recent causal discovery\r\nmethods
on observational iid data generated under different background conditions,\r\nallowing
for violations of the critical assumptions required by each selected approach.
Our experimental findings show that score matching-based methods demonstrate surprising
performance in the false positive and false negative rate of the\r\ninferred graph
in these challenging scenarios, and we provide theoretical insights\r\ninto their
performance. This work is also the first effort to benchmark the stability of\r\ncausal
discovery algorithms with respect to the values of their hyperparameters. Finally,
we hope this paper will set a new standard for the evaluation of causal discovery
methods and can serve as an accessible entry point for practitioners interested\r\nin
the field, highlighting the empirical implications of different algorithm choices."
acknowledgement: "We thank Kun Zhang and Carl-Johann Simon-Gabriel for the insightful
discussions. This work\r\nhas been supported by AFOSR, grant n. FA8655-20-1-7035.
FM is supported by Programma\r\nOperativo Nazionale ricerca e innovazione 2014-2020.
FM partially contributed to this work during an internship at Amazon Web Services
with FL. FL partially contributed while at AWS."
alternative_title:
- Advances in Neural Information Processing Systems
article_processing_charge: No
arxiv: 1
author:
- first_name: Francesco
full_name: Montagna, Francesco
last_name: Montagna
- first_name: Atalanti A.
full_name: Mastakouri, Atalanti A.
last_name: Mastakouri
- first_name: Elias
full_name: Eulig, Elias
last_name: Eulig
- first_name: Nicoletta
full_name: Noceti, Nicoletta
last_name: Noceti
- first_name: Lorenzo
full_name: Rosasco, Lorenzo
last_name: Rosasco
- first_name: Dominik
full_name: Janzing, Dominik
last_name: Janzing
- first_name: Bryon
full_name: Aragam, Bryon
last_name: Aragam
- first_name: Francesco
full_name: Locatello, Francesco
id: 26cfd52f-2483-11ee-8040-88983bcc06d4
last_name: Locatello
orcid: 0000-0002-4850-0683
citation:
ama: 'Montagna F, Mastakouri AA, Eulig E, et al. Assumption violations in causal
discovery and the robustness of score matching. In: 37th Conference on Neural
Information Processing Systems. Vol 36. Neural Information Processing Systems
Foundation; 2023. doi:10.52202/075280-2050'
apa: 'Montagna, F., Mastakouri, A. A., Eulig, E., Noceti, N., Rosasco, L., Janzing,
D., … Locatello, F. (2023). Assumption violations in causal discovery and the
robustness of score matching. In 37th Conference on Neural Information Processing
Systems (Vol. 36). New Orleans, LO, United States: Neural Information Processing
Systems Foundation. https://doi.org/10.52202/075280-2050'
chicago: Montagna, Francesco, Atalanti A. Mastakouri, Elias Eulig, Nicoletta Noceti,
Lorenzo Rosasco, Dominik Janzing, Bryon Aragam, and Francesco Locatello. “Assumption
Violations in Causal Discovery and the Robustness of Score Matching.” In 37th
Conference on Neural Information Processing Systems, Vol. 36. Neural Information
Processing Systems Foundation, 2023. https://doi.org/10.52202/075280-2050.
ieee: F. Montagna et al., “Assumption violations in causal discovery and
the robustness of score matching,” in 37th Conference on Neural Information
Processing Systems, New Orleans, LO, United States, 2023, vol. 36.
ista: 'Montagna F, Mastakouri AA, Eulig E, Noceti N, Rosasco L, Janzing D, Aragam
B, Locatello F. 2023. Assumption violations in causal discovery and the robustness
of score matching. 37th Conference on Neural Information Processing Systems. NeurIPS:
Neural Information Processing Systems, Advances in Neural Information Processing
Systems, vol. 36.'
mla: Montagna, Francesco, et al. “Assumption Violations in Causal Discovery and
the Robustness of Score Matching.” 37th Conference on Neural Information Processing
Systems, vol. 36, Neural Information Processing Systems Foundation, 2023,
doi:10.52202/075280-2050.
short: F. Montagna, A.A. Mastakouri, E. Eulig, N. Noceti, L. Rosasco, D. Janzing,
B. Aragam, F. Locatello, in:, 37th Conference on Neural Information Processing
Systems, Neural Information Processing Systems Foundation, 2023.
conference:
end_date: 2023-12-16
location: New Orleans, LO, United States
name: 'NeurIPS: Neural Information Processing Systems'
start_date: 2023-12-12
das_tickbox: '0'
date_created: 2024-02-07T15:11:56Z
date_published: 2023-12-20T00:00:00Z
date_updated: 2026-08-13T07:51:13Z
day: '20'
ddc:
- '000'
department:
- _id: FrLo
doi: 10.52202/075280-2050
external_id:
arxiv:
- '2310.13387'
file:
- access_level: open_access
checksum: 71d38402b4edef4c084b39f3e2f04526
content_type: application/pdf
creator: dernst
date_created: 2026-08-13T07:49:38Z
date_updated: 2026-08-13T07:49:38Z
file_id: '22701'
file_name: 2023_Neurips_Montagna.pdf
file_size: 7640984
relation: main_file
success: 1
file_date_updated: 2026-08-13T07:49:38Z
has_accepted_license: '1'
intvolume: ' 36'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
publication: 37th Conference on Neural Information Processing Systems
publication_identifier:
eissn:
- 1049-5258
publication_status: published
publisher: Neural Information Processing Systems Foundation
quality_controlled: '1'
researchdata_availability: no
status: public
supplementarymaterial: yes
title: Assumption violations in causal discovery and the robustness of score matching
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2023'
...
---
_id: '14036'
abstract:
- lang: eng
text: Magic-angle spinning (MAS) nuclear magnetic resonance (NMR) is establishing
itself as a powerful method for the characterization of protein dynamics at the
atomic scale. We discuss here how R1ρ MAS relaxation dispersion NMR can explore
microsecond-to-millisecond motions. Progress in instrumentation, isotope labeling,
and pulse sequence design has paved the way for quantitative analyses of even
rare structural fluctuations. In addition to isotropic chemical-shift fluctuations
exploited in solution-state NMR relaxation dispersion experiments, MAS NMR has
a wider arsenal of observables, allowing to see motions even if the exchanging
states do not differ in their chemical shifts. We demonstrate the potential of
the technique for probing motions in challenging large enzymes, membrane proteins,
and protein assemblies.
acknowledgement: We thank Petra Rovó for critical reading of this manuscript. We acknowledge
the Austrian Science Foundation FWF (project AlloSpace, number I5812–B) and funding
by the Institute of Science and Technology Austria.
article_number: '102660'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Federico
full_name: Napoli, Federico
id: d42e08e7-f4fc-11eb-af0a-d71e26138f1b
last_name: Napoli
orcid: 0000-0002-9043-136X
- first_name: Lea Marie
full_name: Becker, Lea Marie
id: 36336939-eb97-11eb-a6c2-c83f1214ca79
last_name: Becker
orcid: 0000-0002-6401-5151
- first_name: Paul
full_name: Schanda, Paul
id: 7B541462-FAF6-11E9-A490-E8DFE5697425
last_name: Schanda
orcid: 0000-0002-9350-7606
citation:
ama: Napoli F, Becker LM, Schanda P. Protein dynamics detected by magic-angle spinning
relaxation dispersion NMR. Current Opinion in Structural Biology. 2023;82(10).
doi:10.1016/j.sbi.2023.102660
apa: Napoli, F., Becker, L. M., & Schanda, P. (2023). Protein dynamics detected
by magic-angle spinning relaxation dispersion NMR. Current Opinion in Structural
Biology. Elsevier. https://doi.org/10.1016/j.sbi.2023.102660
chicago: Napoli, Federico, Lea Marie Becker, and Paul Schanda. “Protein Dynamics
Detected by Magic-Angle Spinning Relaxation Dispersion NMR.” Current Opinion
in Structural Biology. Elsevier, 2023. https://doi.org/10.1016/j.sbi.2023.102660.
ieee: F. Napoli, L. M. Becker, and P. Schanda, “Protein dynamics detected by magic-angle
spinning relaxation dispersion NMR,” Current Opinion in Structural Biology,
vol. 82, no. 10. Elsevier, 2023.
ista: Napoli F, Becker LM, Schanda P. 2023. Protein dynamics detected by magic-angle
spinning relaxation dispersion NMR. Current Opinion in Structural Biology. 82(10),
102660.
mla: Napoli, Federico, et al. “Protein Dynamics Detected by Magic-Angle Spinning
Relaxation Dispersion NMR.” Current Opinion in Structural Biology, vol.
82, no. 10, 102660, Elsevier, 2023, doi:10.1016/j.sbi.2023.102660.
short: F. Napoli, L.M. Becker, P. Schanda, Current Opinion in Structural Biology
82 (2023).
corr_author: '1'
date_created: 2023-08-13T22:01:11Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2025-04-14T09:10:17Z
day: '01'
ddc:
- '570'
department:
- _id: PaSc
doi: 10.1016/j.sbi.2023.102660
external_id:
isi:
- '001053616200001'
pmid:
- '37536064'
file:
- access_level: open_access
checksum: c850f7ac8a4234319755b672c1df69ae
content_type: application/pdf
creator: dernst
date_created: 2024-01-30T12:36:39Z
date_updated: 2024-01-30T12:36:39Z
file_id: '14907'
file_name: 2023_CurrentOpinionStrucBio_Napoli.pdf
file_size: 1231998
relation: main_file
success: 1
file_date_updated: 2024-01-30T12:36:39Z
intvolume: ' 82'
isi: 1
issue: '10'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: eb9c82eb-77a9-11ec-83b8-aadd536561cf
grant_number: I05812
name: AlloSpace. The emergence and mechanisms of allostery
publication: Current Opinion in Structural Biology
publication_identifier:
eissn:
- 1879-033X
issn:
- 0959-440X
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Protein dynamics detected by magic-angle spinning relaxation dispersion NMR
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 82
year: '2023'
...
---
_id: '14042'
abstract:
- lang: eng
text: Long-time and large-data existence of weak solutions for initial- and boundary-value
problems concerning three-dimensional flows of incompressible fluids is nowadays
available not only for Navier–Stokes fluids but also for various fluid models
where the relation between the Cauchy stress tensor and the symmetric part of
the velocity gradient is nonlinear. The majority of such studies however concerns
models where such a dependence is explicit (the stress is a function of the velocity
gradient), which makes the class of studied models unduly restrictive. The same
concerns boundary conditions, or more precisely the slipping mechanisms on the
boundary, where the no-slip is still the most preferred condition considered in
the literature. Our main objective is to develop a robust mathematical theory
for unsteady internal flows of implicitly constituted incompressible fluids with
implicit relations between the tangential projections of the velocity and the
normal traction on the boundary. The theory covers numerous rheological models
used in chemistry, biorheology, polymer and food industry as well as in geomechanics.
It also includes, as special cases, nonlinear slip as well as stick–slip boundary
conditions. Unlike earlier studies, the conditions characterizing admissible classes
of constitutive equations are expressed by means of tools of elementary calculus.
In addition, a fully constructive proof (approximation scheme) is incorporated.
Finally, we focus on the question of uniqueness of such weak solutions.
acknowledgement: "M. Bulíček and J. Málek acknowledge the support of the project No.
20-11027X financed by the Czech Science foundation (GAČR). M. Bulíček and J. Málek
are members of the Nečas Center for Mathematical Modelling.\r\nOpen access publishing
supported by the National Technical Library in Prague."
article_number: '72'
article_processing_charge: Yes (via OA deal)
article_type: original
arxiv: 1
author:
- first_name: Miroslav
full_name: Bulíček, Miroslav
last_name: Bulíček
- first_name: Josef
full_name: Málek, Josef
last_name: Málek
- first_name: Erika
full_name: Maringová, Erika
id: dbabca31-66eb-11eb-963a-fb9c22c880b4
last_name: Maringová
citation:
ama: Bulíček M, Málek J, Maringová E. On unsteady internal flows of incompressible
fluids characterized by implicit constitutive equations in the bulk and on the
boundary. Journal of Mathematical Fluid Mechanics. 2023;25(3). doi:10.1007/s00021-023-00803-w
apa: Bulíček, M., Málek, J., & Maringová, E. (2023). On unsteady internal flows
of incompressible fluids characterized by implicit constitutive equations in the
bulk and on the boundary. Journal of Mathematical Fluid Mechanics. Springer
Nature. https://doi.org/10.1007/s00021-023-00803-w
chicago: Bulíček, Miroslav, Josef Málek, and Erika Maringová. “On Unsteady Internal
Flows of Incompressible Fluids Characterized by Implicit Constitutive Equations
in the Bulk and on the Boundary.” Journal of Mathematical Fluid Mechanics.
Springer Nature, 2023. https://doi.org/10.1007/s00021-023-00803-w.
ieee: M. Bulíček, J. Málek, and E. Maringová, “On unsteady internal flows of incompressible
fluids characterized by implicit constitutive equations in the bulk and on the
boundary,” Journal of Mathematical Fluid Mechanics, vol. 25, no. 3. Springer
Nature, 2023.
ista: Bulíček M, Málek J, Maringová E. 2023. On unsteady internal flows of incompressible
fluids characterized by implicit constitutive equations in the bulk and on the
boundary. Journal of Mathematical Fluid Mechanics. 25(3), 72.
mla: Bulíček, Miroslav, et al. “On Unsteady Internal Flows of Incompressible Fluids
Characterized by Implicit Constitutive Equations in the Bulk and on the Boundary.”
Journal of Mathematical Fluid Mechanics, vol. 25, no. 3, 72, Springer Nature,
2023, doi:10.1007/s00021-023-00803-w.
short: M. Bulíček, J. Málek, E. Maringová, Journal of Mathematical Fluid Mechanics
25 (2023).
date_created: 2023-08-13T22:01:13Z
date_published: 2023-08-01T00:00:00Z
date_updated: 2026-08-18T07:47:38Z
day: '01'
ddc:
- '510'
department:
- _id: JuFi
doi: 10.1007/s00021-023-00803-w
external_id:
arxiv:
- '2301.12834'
isi:
- '001040354900001'
file:
- access_level: open_access
checksum: c549cd8f0dd02ed60477a05ca045f481
content_type: application/pdf
creator: dernst
date_created: 2023-08-14T07:24:17Z
date_updated: 2023-08-14T07:24:17Z
file_id: '14046'
file_name: 2023_JourMathFluidMech_Bulicek.pdf
file_size: 845748
relation: main_file
success: 1
file_date_updated: 2023-08-14T07:24:17Z
has_accepted_license: '1'
intvolume: ' 25'
isi: 1
issue: '3'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
publication: Journal of Mathematical Fluid Mechanics
publication_identifier:
eissn:
- 1422-6952
issn:
- 1422-6928
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: On unsteady internal flows of incompressible fluids characterized by implicit
constitutive equations in the bulk and on the boundary
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2023'
...
---
_id: '14691'
abstract:
- lang: eng
text: "Continuous Group-Key Agreement (CGKA) allows a group of users to maintain
a shared key. It is the fundamental cryptographic primitive underlying group messaging
schemes and related protocols, most notably TreeKEM, the underlying key agreement
protocol of the Messaging Layer Security (MLS) protocol, a standard for group
messaging by the IETF. CKGA works in an asynchronous setting where parties only
occasionally must come online, and their messages are relayed by an untrusted
server. The most expensive operation provided by CKGA is that which allows for
a user to refresh their key material in order to achieve forward secrecy (old
messages are secure when a user is compromised) and post-compromise security (users
can heal from compromise). One caveat of early CGKA protocols is that these update
operations had to be performed sequentially, with any user wanting to update their
key material having had to receive and process all previous updates. Late versions
of TreeKEM do allow for concurrent updates at the cost of a communication overhead
per update message that is linear in the number of updating parties. This was
shown to be indeed necessary when achieving PCS in just two rounds of communication
by [Bienstock et al. TCC’20].\r\nThe recently proposed protocol CoCoA [Alwen et
al. Eurocrypt’22], however, shows that this overhead can be reduced if PCS requirements
are relaxed, and only a logarithmic number of rounds is required. The natural
question, thus, is whether CoCoA is optimal in this setting.\r\nIn this work we
answer this question, providing a lower bound on the cost (concretely, the amount
of data to be uploaded to the server) for CGKA protocols that heal in an arbitrary
k number of rounds, that shows that CoCoA is very close to optimal. Additionally,
we extend CoCoA to heal in an arbitrary number of rounds, and propose a modification
of it, with a reduced communication cost for certain k.\r\nWe prove our bound
in a combinatorial setting where the state of the protocol progresses in rounds,
and the state of the protocol in each round is captured by a set system, each
set specifying a set of users who share a secret key. We show this combinatorial
model is equivalent to a symbolic model capturing building blocks including PRFs
and public-key encryption, related to the one used by Bienstock et al.\r\nOur
lower bound is of order k•n1+1/(k-1)/log(k), where 2≤k≤log(n) is the number of
updates per user the protocol requires to heal. This generalizes the n2 bound
for k=2 from Bienstock et al.. This bound almost matches the k⋅n1+2/(k-1) or k2⋅n1+1/(k-1)
efficiency we get for the variants of the CoCoA protocol also introduced in this
paper."
alternative_title:
- LNCS
article_processing_charge: No
author:
- first_name: Benedikt
full_name: Auerbach, Benedikt
id: D33D2B18-E445-11E9-ABB7-15F4E5697425
last_name: Auerbach
orcid: 0000-0002-7553-6606
- first_name: Miguel
full_name: Cueto Noval, Miguel
id: ffc563a3-f6e0-11ea-865d-e3cce03d17cc
last_name: Cueto Noval
orcid: 0000-0002-2505-4246
- first_name: Guillermo
full_name: Pascual Perez, Guillermo
id: 2D7ABD02-F248-11E8-B48F-1D18A9856A87
last_name: Pascual Perez
orcid: 0000-0001-8630-415X
- first_name: Krzysztof Z
full_name: Pietrzak, Krzysztof Z
id: 3E04A7AA-F248-11E8-B48F-1D18A9856A87
last_name: Pietrzak
orcid: 0000-0002-9139-1654
citation:
ama: 'Auerbach B, Cueto Noval M, Pascual Perez G, Pietrzak KZ. On the cost of post-compromise
security in concurrent Continuous Group-Key Agreement. In: 21st International
Conference on Theory of Cryptography. Vol 14371. Springer Nature; 2023:271-300.
doi:10.1007/978-3-031-48621-0_10'
apa: 'Auerbach, B., Cueto Noval, M., Pascual Perez, G., & Pietrzak, K. Z. (2023).
On the cost of post-compromise security in concurrent Continuous Group-Key Agreement.
In 21st International Conference on Theory of Cryptography (Vol. 14371,
pp. 271–300). Taipei, Taiwan: Springer Nature. https://doi.org/10.1007/978-3-031-48621-0_10'
chicago: Auerbach, Benedikt, Miguel Cueto Noval, Guillermo Pascual Perez, and Krzysztof
Z Pietrzak. “On the Cost of Post-Compromise Security in Concurrent Continuous
Group-Key Agreement.” In 21st International Conference on Theory of Cryptography,
14371:271–300. Springer Nature, 2023. https://doi.org/10.1007/978-3-031-48621-0_10.
ieee: B. Auerbach, M. Cueto Noval, G. Pascual Perez, and K. Z. Pietrzak, “On the cost
of post-compromise security in concurrent Continuous Group-Key Agreement,” in
21st International Conference on Theory of Cryptography, Taipei, Taiwan,
2023, vol. 14371, pp. 271–300.
ista: 'Auerbach B, Cueto Noval M, Pascual Perez G, Pietrzak KZ. 2023. On the cost
of post-compromise security in concurrent Continuous Group-Key Agreement. 21st
International Conference on Theory of Cryptography. TCC: Theory of Cryptography,
LNCS, vol. 14371, 271–300.'
mla: Auerbach, Benedikt, et al. “On the Cost of Post-Compromise Security in Concurrent
Continuous Group-Key Agreement.” 21st International Conference on Theory of
Cryptography, vol. 14371, Springer Nature, 2023, pp. 271–300, doi:10.1007/978-3-031-48621-0_10.
short: B. Auerbach, M. Cueto Noval, G. Pascual Perez, K.Z. Pietrzak, in:, 21st International
Conference on Theory of Cryptography, Springer Nature, 2023, pp. 271–300.
conference:
end_date: 2023-12-02
location: Taipei, Taiwan
name: 'TCC: Theory of Cryptography'
start_date: 2023-11-29
corr_author: '1'
date_created: 2023-12-17T23:00:53Z
date_published: 2023-11-27T00:00:00Z
date_updated: 2026-08-21T10:53:16Z
day: '27'
department:
- _id: KrPi
doi: 10.1007/978-3-031-48621-0_10
external_id:
isi:
- '001160724400010'
intvolume: ' 14371'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://eprint.iacr.org/2023/1123
month: '11'
oa: 1
oa_version: Preprint
page: 271-300
publication: 21st International Conference on Theory of Cryptography
publication_identifier:
eissn:
- 1611-3349
isbn:
- '9783031486203'
issn:
- 0302-9743
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '22664'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: On the cost of post-compromise security in concurrent Continuous Group-Key
Agreement
type: conference
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 14371
year: '2023'
...
---
_id: '12334'
abstract:
- lang: eng
text: Regulation of the Arp2/3 complex is required for productive nucleation of
branched actin networks. An emerging aspect of regulation is the incorporation
of subunit isoforms into the Arp2/3 complex. Specifically, both ArpC5 subunit
isoforms, ArpC5 and ArpC5L, have been reported to fine-tune nucleation activity
and branch junction stability. We have combined reverse genetics and cellular
structural biology to describe how ArpC5 and ArpC5L differentially affect cell
migration. Both define the structural stability of ArpC1 in branch junctions and,
in turn, by determining protrusion characteristics, affect protein dynamics and
actin network ultrastructure. ArpC5 isoforms also affect the positioning of members
of the Ena/Vasodilator-stimulated phosphoprotein (VASP) family of actin filament
elongators, which mediate ArpC5 isoform–specific effects on the actin assembly
level. Our results suggest that ArpC5 and Ena/VASP proteins are part of a signaling
pathway enhancing cell migration.
acknowledged_ssus:
- _id: ScienComp
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
acknowledgement: "We would like to thank K. von Peinen and B. Denker (Helmholtz Centre
for Infection Research, Braunschweig, Germany) for experimental and technical assistance,
respectively.\r\nThis research was supported by the Scientific Service Units (SSUs)
of ISTA through resources provided by Scientific Computing (SciComp), the Life Science
Facility (LSF), the Imaging and Optics facility (IOF), and the Electron Microscopy
Facility (EMF). We acknowledge support from ISTA and from the Austrian Science Fund
(FWF) (P33367) to F.K.M.S., from the Research Training Group GRK2223 and the Helmholtz
Society to K.R,. and from the Deutsche Forschungsgemeinschaft (DFG) to J.F. and
K.R."
article_number: add6495
article_processing_charge: No
article_type: original
author:
- first_name: Florian
full_name: Fäßler, Florian
id: 404F5528-F248-11E8-B48F-1D18A9856A87
last_name: Fäßler
orcid: 0000-0001-7149-769X
- first_name: Manjunath
full_name: Javoor, Manjunath
id: 305ab18b-dc7d-11ea-9b2f-b58195228ea2
last_name: Javoor
orcid: 0000-0003-2311-2112
- first_name: Julia
full_name: Datler, Julia
id: 3B12E2E6-F248-11E8-B48F-1D18A9856A87
last_name: Datler
orcid: 0000-0002-3616-8580
- first_name: Hermann
full_name: Döring, Hermann
last_name: Döring
- first_name: Florian
full_name: Hofer, Florian
id: b9d234ba-9e33-11ed-95b6-cd561df280e6
last_name: Hofer
- first_name: Georgi A
full_name: Dimchev, Georgi A
id: 38C393BE-F248-11E8-B48F-1D18A9856A87
last_name: Dimchev
orcid: 0000-0001-8370-6161
- first_name: Victor-Valentin
full_name: Hodirnau, Victor-Valentin
id: 3661B498-F248-11E8-B48F-1D18A9856A87
last_name: Hodirnau
orcid: 0000-0003-3904-947X
- first_name: Jan
full_name: Faix, Jan
last_name: Faix
- first_name: Klemens
full_name: Rottner, Klemens
last_name: Rottner
- first_name: Florian KM
full_name: Schur, Florian KM
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
citation:
ama: Fäßler F, Javoor M, Datler J, et al. ArpC5 isoforms regulate Arp2/3 complex–dependent
protrusion through differential Ena/VASP positioning. Science Advances.
2023;9(3). doi:10.1126/sciadv.add6495
apa: Fäßler, F., Javoor, M., Datler, J., Döring, H., Hofer, F., Dimchev, G. A.,
… Schur, F. K. (2023). ArpC5 isoforms regulate Arp2/3 complex–dependent protrusion
through differential Ena/VASP positioning. Science Advances. American Association
for the Advancement of Science. https://doi.org/10.1126/sciadv.add6495
chicago: Fäßler, Florian, Manjunath Javoor, Julia Datler, Hermann Döring, Florian
Hofer, Georgi A Dimchev, Victor-Valentin Hodirnau, Jan Faix, Klemens Rottner,
and Florian KM Schur. “ArpC5 Isoforms Regulate Arp2/3 Complex–Dependent Protrusion
through Differential Ena/VASP Positioning.” Science Advances. American
Association for the Advancement of Science, 2023. https://doi.org/10.1126/sciadv.add6495.
ieee: F. Fäßler et al., “ArpC5 isoforms regulate Arp2/3 complex–dependent
protrusion through differential Ena/VASP positioning,” Science Advances,
vol. 9, no. 3. American Association for the Advancement of Science, 2023.
ista: Fäßler F, Javoor M, Datler J, Döring H, Hofer F, Dimchev GA, Hodirnau V-V,
Faix J, Rottner K, Schur FK. 2023. ArpC5 isoforms regulate Arp2/3 complex–dependent
protrusion through differential Ena/VASP positioning. Science Advances. 9(3),
add6495.
mla: Fäßler, Florian, et al. “ArpC5 Isoforms Regulate Arp2/3 Complex–Dependent Protrusion
through Differential Ena/VASP Positioning.” Science Advances, vol. 9, no.
3, add6495, American Association for the Advancement of Science, 2023, doi:10.1126/sciadv.add6495.
short: F. Fäßler, M. Javoor, J. Datler, H. Döring, F. Hofer, G.A. Dimchev, V.-V.
Hodirnau, J. Faix, K. Rottner, F.K. Schur, Science Advances 9 (2023).
corr_author: '1'
date_created: 2023-01-23T07:26:42Z
date_published: 2023-01-20T00:00:00Z
date_updated: 2026-08-31T07:18:54Z
day: '20'
ddc:
- '570'
department:
- _id: FlSc
- _id: EM-Fac
doi: 10.1126/sciadv.add6495
external_id:
isi:
- '000964550100015'
pmid:
- '36662867'
file:
- access_level: open_access
checksum: ce81a6d0b84170e5e8c62f6acfa15d9e
content_type: application/pdf
creator: dernst
date_created: 2023-01-23T07:45:54Z
date_updated: 2023-01-23T07:45:54Z
file_id: '12335'
file_name: 2023_ScienceAdvances_Faessler.pdf
file_size: 1756234
relation: main_file
success: 1
file_date_updated: 2023-01-23T07:45:54Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
issue: '3'
keyword:
- Multidisciplinary
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 9B954C5C-BA93-11EA-9121-9846C619BF3A
grant_number: P33367
name: Structure and isoform diversity of the Arp2/3 complex
publication: Science Advances
publication_identifier:
issn:
- 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
related_material:
record:
- id: '14562'
relation: research_data
status: public
- id: '18766'
relation: dissertation_contains
status: public
- id: '22744'
relation: dissertation_contains
status: for_moderation
scopus_import: '1'
status: public
title: ArpC5 isoforms regulate Arp2/3 complex–dependent protrusion through differential
Ena/VASP positioning
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2023'
...
---
_id: '12421'
abstract:
- lang: eng
text: The actin cytoskeleton plays a key role in cell migration and cellular morphodynamics
in most eukaryotes. The ability of the actin cytoskeleton to assemble and disassemble
in a spatiotemporally controlled manner allows it to form higher-order structures,
which can generate forces required for a cell to explore and navigate through
its environment. It is regulated not only via a complex synergistic and competitive
interplay between actin-binding proteins (ABP), but also by filament biochemistry
and filament geometry. The lack of structural insights into how geometry and ABPs
regulate the actin cytoskeleton limits our understanding of the molecular mechanisms
that define actin cytoskeleton remodeling and, in turn, impact emerging cell migration
characteristics. With the advent of cryo-electron microscopy (cryo-EM) and advanced
computational methods, it is now possible to define these molecular mechanisms
involving actin and its interactors at both atomic and ultra-structural levels
in vitro and in cellulo. In this review, we will provide an overview of the available
cryo-EM methods, applicable to further our understanding of the actin cytoskeleton,
specifically in the context of cell migration. We will discuss how these methods
have been employed to elucidate ABP- and geometry-defined regulatory mechanisms
in initiating, maintaining, and disassembling cellular actin networks in migratory
protrusions.
acknowledgement: 'We apologize for not being able to mention and cite additional excellent
work that would have fit the scope of this review, due to space restraints. We thank
Jesse Hansen for comments on the manuscript. We acknowledge support from the Austrian
Science Fund (FWF): P33367 and the Institute of Science and Technology Austria.'
article_processing_charge: No
article_type: original
author:
- first_name: Florian
full_name: Fäßler, Florian
id: 404F5528-F248-11E8-B48F-1D18A9856A87
last_name: Fäßler
orcid: 0000-0001-7149-769X
- first_name: Manjunath
full_name: Javoor, Manjunath
id: 305ab18b-dc7d-11ea-9b2f-b58195228ea2
last_name: Javoor
orcid: 0000-0003-2311-2112
- first_name: Florian KM
full_name: Schur, Florian KM
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
citation:
ama: Fäßler F, Javoor M, Schur FK. Deciphering the molecular mechanisms of actin
cytoskeleton regulation in cell migration using cryo-EM. Biochemical Society
Transactions. 2023;51(1):87-99. doi:10.1042/bst20220221
apa: Fäßler, F., Javoor, M., & Schur, F. K. (2023). Deciphering the molecular
mechanisms of actin cytoskeleton regulation in cell migration using cryo-EM. Biochemical
Society Transactions. Portland Press. https://doi.org/10.1042/bst20220221
chicago: Fäßler, Florian, Manjunath Javoor, and Florian KM Schur. “Deciphering the
Molecular Mechanisms of Actin Cytoskeleton Regulation in Cell Migration Using
Cryo-EM.” Biochemical Society Transactions. Portland Press, 2023. https://doi.org/10.1042/bst20220221.
ieee: F. Fäßler, M. Javoor, and F. K. Schur, “Deciphering the molecular mechanisms
of actin cytoskeleton regulation in cell migration using cryo-EM,” Biochemical
Society Transactions, vol. 51, no. 1. Portland Press, pp. 87–99, 2023.
ista: Fäßler F, Javoor M, Schur FK. 2023. Deciphering the molecular mechanisms of
actin cytoskeleton regulation in cell migration using cryo-EM. Biochemical Society
Transactions. 51(1), 87–99.
mla: Fäßler, Florian, et al. “Deciphering the Molecular Mechanisms of Actin Cytoskeleton
Regulation in Cell Migration Using Cryo-EM.” Biochemical Society Transactions,
vol. 51, no. 1, Portland Press, 2023, pp. 87–99, doi:10.1042/bst20220221.
short: F. Fäßler, M. Javoor, F.K. Schur, Biochemical Society Transactions 51 (2023)
87–99.
corr_author: '1'
date_created: 2023-01-27T10:08:19Z
date_published: 2023-02-01T00:00:00Z
date_updated: 2026-08-31T07:18:54Z
day: '01'
ddc:
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pmid:
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oa: 1
oa_version: Published Version
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scopus_import: '1'
status: public
title: Deciphering the molecular mechanisms of actin cytoskeleton regulation in cell
migration using cryo-EM
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 51
year: '2023'
...
---
OA_place: publisher
_id: '12491'
abstract:
- lang: eng
text: "The extracellular matrix (ECM) is a hydrated and complex three-dimensional
network consisting of proteins, polysaccharides, and water. It provides structural
scaffolding for the cells embedded within it and is essential in regulating numerous
physiological processes, including cell migration and proliferation, wound healing,
and stem cell fate. \r\nDespite extensive study, detailed structural knowledge
of ECM components in physiologically relevant conditions is still rudimentary.
This is due to methodological limitations in specimen preparation protocols which
are incompatible with keeping large samples, such as the ECM, in their native
state for subsequent imaging. Conventional electron microscopy (EM) techniques
rely on fixation, dehydration, contrasting, and sectioning. This results in the
alteration of a highly hydrated environment and the potential introduction of
artifacts. Other structural biology techniques, such as nuclear magnetic resonance
(NMR) spectroscopy and X-ray crystallography, allow high-resolution analysis of
protein structures but only work on homogenous and purified samples, hence lacking
contextual information. Currently, no approach exists for the ultrastructural
and structural study of extracellular components under native conditions in a
physiological, 3D environment. \r\nIn this thesis, I have developed a workflow
that allows for the ultrastructural analysis of the ECM in near-native conditions
at molecular resolution. The developments I introduced include implementing a
novel specimen preparation workflow for cell-derived matrices (CDMs) to render
them compatible with ion-beam milling and subsequent high-resolution cryo-electron
tomography (ET). \r\nTo this end, I have established protocols to generate CDMs
grown over several weeks on EM grids that are compatible with downstream cryo-EM
sample preparation and imaging techniques. Characterization of these ECMs confirmed
that they contain essential ECM components such as collagen I, collagen VI, and
fibronectin I in high abundance and hence represent a bona fide biologically-relevant
sample. I successfully optimized vitrification of these specimens by testing various
vitrification techniques and cryoprotectants. \r\nIn order to obtain high-resolution
molecular insights into the ultrastructure and organization of CDMs, I established
cryo-focused ion beam scanning electron microscopy (FIBSEM) on these challenging
and complex specimens. I explored different approaches for the creation of thin
cryo-lamellae by FIB milling and succeeded in optimizing the cryo-lift-out technique,
resulting in high-quality lamellae of approximately 200 nm thickness. \r\nHigh-resolution
Cryo-ET of these lamellae revealed for the first time the architecture of native
CDM in the context of matrix-secreting cells. This allowed for the in situ visualization
of fibrillar matrix proteins such as collagen, laying the foundation for future
structural and ultrastructural characterization of these proteins in their near-native
environment. \r\nIn summary, in this thesis, I present a novel workflow that combines
state-of-the-art cryo-EM specimen preparation and imaging technologies to permit
characterization of the ECM, an important tissue component in higher organisms.
This innovative and highly versatile workflow will enable addressing far-reaching
questions on ECM architecture, composition, and reciprocal ECM-cell interactions."
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: Bio
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Bettina
full_name: Zens, Bettina
id: 45FD126C-F248-11E8-B48F-1D18A9856A87
last_name: Zens
orcid: 0000-0002-9561-1239
citation:
ama: Zens B. Ultrastructural characterization of natively preserved extracellular
matrix by cryo-electron tomography. 2023. doi:10.15479/at:ista:12491
apa: Zens, B. (2023). Ultrastructural characterization of natively preserved
extracellular matrix by cryo-electron tomography. Institute of Science and
Technology Austria. https://doi.org/10.15479/at:ista:12491
chicago: Zens, Bettina. “Ultrastructural Characterization of Natively Preserved
Extracellular Matrix by Cryo-Electron Tomography.” Institute of Science and Technology
Austria, 2023. https://doi.org/10.15479/at:ista:12491.
ieee: B. Zens, “Ultrastructural characterization of natively preserved extracellular
matrix by cryo-electron tomography,” Institute of Science and Technology Austria,
2023.
ista: Zens B. 2023. Ultrastructural characterization of natively preserved extracellular
matrix by cryo-electron tomography. Institute of Science and Technology Austria.
mla: Zens, Bettina. Ultrastructural Characterization of Natively Preserved Extracellular
Matrix by Cryo-Electron Tomography. Institute of Science and Technology Austria,
2023, doi:10.15479/at:ista:12491.
short: B. Zens, Ultrastructural Characterization of Natively Preserved Extracellular
Matrix by Cryo-Electron Tomography, Institute of Science and Technology Austria,
2023.
corr_author: '1'
date_created: 2023-02-02T14:50:20Z
date_published: 2023-02-02T00:00:00Z
date_updated: 2026-04-07T13:49:23Z
day: '02'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: FlSc
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keyword:
- cryo-EM
- cryo-ET
- FIB milling
- method development
- FIBSEM
- extracellular matrix
- ECM
- cell-derived matrices
- CDMs
- cell culture
- high pressure freezing
- HPF
- structural biology
- tomography
- collagen
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: '187'
project:
- _id: eba3b5f6-77a9-11ec-83b8-cf0905748aa3
name: Integrated visual proteomics of reciprocal cell-extracellular matrix interactions
- _id: 059B463C-7A3F-11EA-A408-12923DDC885E
name: "NÃ\x96-Fonds Preis für die Jungforscherin des Jahres am IST Austria"
publication_identifier:
isbn:
- 978-3-99078-027-5
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
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relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Florian KM
full_name: Schur, Florian KM
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
title: Ultrastructural characterization of natively preserved extracellular matrix
by cryo-electron tomography
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2023'
...
---
OA_place: publisher
_id: '13984'
abstract:
- lang: eng
text: "Social insects fight disease using their individual immune systems and the
cooperative\r\nsanitary behaviors of colony members. These social defenses are
well explored against\r\nexternally-infecting pathogens, but little is known about
defense strategies against\r\ninternally-infecting pathogens, such as viruses.
Viruses are ubiquitous and in the last decades\r\nit has become evident that also
many ant species harbor viruses. We present one of the first\r\nstudies addressing
transmission dynamics and collective disease defenses against viruses in\r\nants
on a mechanistic level. I successfully established an experimental ant host –
viral\r\npathogen system as a model for the defense strategies used by social
insects against internal\r\npathogen infections, as outlined in the third chapter.
In particular, we studied how garden ants\r\n(Lasius neglectus) defend themselves
and their colonies against the generalist insect virus\r\nCrPV (cricket paralysis
virus). We chose microinjections of virus directly into the ants’\r\nhemolymph
because it allowed us to use a defined exposure dose. Here we show that this is
a\r\ngood model system, as the virus is replicating and thus infecting the host.
The ants mount a\r\nclear individual immune response against the viral infection,
which is characterized by a\r\nspecific siRNA pattern, namely siRNAs mapping against
the viral genome with a peak of 21\r\nand 22 bp long fragments. The onset of this
immune response is consistent with the timeline\r\nof viral replication that starts
already within two days post injection. The disease manifests in\r\ndecreased
survival over a course of two to three weeks.\r\nRegarding group living, we find
that infected ants show a strong individual immune response,\r\nbut that their
course of disease is little affected by nestmate presence, as described in chapter\r\nfour.
Hence, we do not find social immunity in the context of viral infections in ants.\r\nNestmates,
however, can contract the virus. Using Drosophila S2R+ cells in culture, we\r\nshowed
that 94 % of the nestmates contract active virus within four days of social contact
to\r\nan infected individual. Virus is transmitted in low doses, thus not causing
disease\r\ntransmission within the colony. While virus can be transmitted during
short direct contacts,\r\nwe also assume transmission from deceased ants and show
that the nestmates’ immune\r\nsystem gets activated after contracting a low viral
dose. We find considerable potential for\r\nindirect transmission via the nest
space. Virus is shed to the nest, where it stays viable for one\r\nweek and is
also picked up by other ants. Apart from that, we want to underline the potential\r\nof
ant poison as antiviral agent. We determined that ant poison successfully inactivates
CrPV\r\nin vitro. However, we found no evidence for effective poison use to sanitize
the nest space.\r\nOn the other hand, local application of ant poison by oral
poison uptake, which is part of the\r\nants prophylactic behavioral repertoire,
probably contributes to keeping the gut of each\r\nindividual sanitized. We hypothesize
that oral poison uptake might be the reason why we did\r\nnot find viable virus
in the trophallactic fluid.\r\nThe fifth chapter encompasses preliminary data
on potential social immunization. However,\r\nour experiments do not confirm an
actual survival benefit for the nestmates upon pathogen\r\nchallenge under the
given experimental settings. Nevertheless, we do not want to rule out the\r\npossibility
for nestmate immunization, but rather emphasize that considering different\r\nexperimental
timelines and viral doses would provide a multitude of options for follow-up\r\nexperiments.\r\nIn
conclusion, we find that prophylactic individual behaviors, such as oral poison
uptake,\r\nmight play a role in preventing viral disease transmission. Compared
to colony defense\r\nagainst external pathogens, internal pathogen infections
require a stronger component of\r\nindividual physiological immunity than behavioral
social immunity, yet could still lead to\r\ncollective protection."
acknowledged_ssus:
- _id: LifeSc
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Anna
full_name: Franschitz, Anna
id: 480826C8-F248-11E8-B48F-1D18A9856A87
last_name: Franschitz
citation:
ama: Franschitz A. Individual and social immunity against viral infections in ants.
2023. doi:10.15479/at:ista:13984
apa: Franschitz, A. (2023). Individual and social immunity against viral infections
in ants. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:13984
chicago: Franschitz, Anna. “Individual and Social Immunity against Viral Infections
in Ants.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:13984.
ieee: A. Franschitz, “Individual and social immunity against viral infections in
ants,” Institute of Science and Technology Austria, 2023.
ista: Franschitz A. 2023. Individual and social immunity against viral infections
in ants. Institute of Science and Technology Austria.
mla: Franschitz, Anna. Individual and Social Immunity against Viral Infections
in Ants. Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:13984.
short: A. Franschitz, Individual and Social Immunity against Viral Infections in
Ants, Institute of Science and Technology Austria, 2023.
corr_author: '1'
date_created: 2023-08-08T15:33:29Z
date_published: 2023-08-08T00:00:00Z
date_updated: 2026-04-07T13:51:29Z
day: '08'
ddc:
- '570'
- '577'
degree_awarded: PhD
department:
- _id: GradSch
- _id: SyCr
doi: 10.15479/at:ista:13984
file:
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date_created: 2024-03-01T08:37:15Z
date_updated: 2024-10-29T23:31:04Z
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has_accepted_license: '1'
language:
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month: '08'
oa: 1
oa_version: Published Version
page: '89'
publication_identifier:
isbn:
- 978-3-99078-034-3
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Sylvia
full_name: Cremer, Sylvia
id: 2F64EC8C-F248-11E8-B48F-1D18A9856A87
last_name: Cremer
orcid: 0000-0002-2193-3868
title: Individual and social immunity against viral infections in ants
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2023'
...
---
OA_place: publisher
_id: '12964'
abstract:
- lang: eng
text: "Pattern formation is of great importance for its contribution across different
biological behaviours. During developmental processes for example, patterns of
chemical gradients are\r\nestablished to determine cell fate and complex tissue
patterns emerge to define structures such\r\nas limbs and vascular networks. Patterns
are also seen in collectively migrating groups, for\r\ninstance traveling waves
of density emerging in moving animal flocks as well as collectively migrating
cells and tissues. To what extent these biological patterns arise spontaneously
through\r\nthe local interaction of individual constituents or are dictated by
higher level instructions is\r\nstill an open question however there is evidence
for the involvement of both types of process.\r\nWhere patterns arise spontaneously
there is a long standing interest in how far the interplay\r\nof mechanics, e.g.
force generation and deformation, and chemistry, e.g. gene regulation\r\nand signaling,
contributes to the behaviour. This is because many systems are able to both\r\nchemically
regulate mechanical force production and chemically sense mechanical deformation,\r\nforming
mechano-chemical feedback loops which can potentially become unstable towards\r\nspatio
and/or temporal patterning.\r\nWe work with experimental collaborators to investigate
the possibility that this type of\r\ninteraction drives pattern formation in biological
systems at different scales. We focus first on\r\ntissue-level ERK-density waves
observed during the wound healing response across different\r\nsystems where many
previous studies have proposed that patterns depend on polarized cell\r\nmigration
and arise from a mechanical flocking-like mechanism. By combining theory with\r\nmechanical
and optogenetic perturbation experiments on in vitro monolayers we instead find\r\nevidence
for mechanochemical pattern formation involving only scalar bilateral feedbacks\r\nbetween
ERK signaling and cell contraction. We perform further modeling and experiment\r\nto
study how this instability couples with polar cell migration in order to produce
a robust\r\nand efficient wound healing response. In a following chapter we implement
ERK-density\r\ncoupling and cell migration in a 2D active vertex model to investigate
the interaction of\r\nERK-density patterning with different tissue rheologies
and find that the spatio-temporal\r\ndynamics are able to both locally and globally
fluidize a tissue across the solid-fluid glass\r\ntransition. In a last chapter
we move towards lower spatial scales in the context of subcellular\r\npatterning
of the cell cytoskeleton where we investigate the transition between phases of\r\nspatially
homogeneous temporal oscillations and chaotic spatio-temporal patterning in the\r\ndynamics
of myosin and ROCK activities (a motor component of the actomyosin cytoskeleton\r\nand
its activator). Experimental evidence supports an intrinsic chemical oscillator
which we\r\nencode in a reaction model and couple to a contractile active gel
description of the cell cortex.\r\nThe model exhibits phases of chemical oscillations
and contractile spatial patterning which\r\nreproduce many features of the dynamics
seen in Drosophila oocyte epithelia in vivo. However,\r\nadditional pharmacological
perturbations to inhibit myosin contractility leaves the role of\r\ncontractile
instability unclear. We discuss alternative hypotheses and investigate the possibility\r\nof
reaction-diffusion instability."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Daniel R
full_name: Boocock, Daniel R
id: 453AF628-F248-11E8-B48F-1D18A9856A87
last_name: Boocock
orcid: 0000-0002-1585-2631
citation:
ama: Boocock DR. Mechanochemical pattern formation across biological scales. 2023.
doi:10.15479/at:ista:12964
apa: Boocock, D. R. (2023). Mechanochemical pattern formation across biological
scales. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12964
chicago: Boocock, Daniel R. “Mechanochemical Pattern Formation across Biological
Scales.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12964.
ieee: D. R. Boocock, “Mechanochemical pattern formation across biological scales,”
Institute of Science and Technology Austria, 2023.
ista: Boocock DR. 2023. Mechanochemical pattern formation across biological scales.
Institute of Science and Technology Austria.
mla: Boocock, Daniel R. Mechanochemical Pattern Formation across Biological Scales.
Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:12964.
short: D.R. Boocock, Mechanochemical Pattern Formation across Biological Scales,
Institute of Science and Technology Austria, 2023.
corr_author: '1'
date_created: 2023-05-15T14:52:36Z
date_published: 2023-05-17T00:00:00Z
date_updated: 2026-04-07T13:52:57Z
day: '17'
ddc:
- '530'
degree_awarded: PhD
department:
- _id: GradSch
- _id: EdHa
doi: 10.15479/at:ista:12964
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language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: '146'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication_identifier:
isbn:
- 978-3-99078-032-9
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '8602'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
title: Mechanochemical pattern formation across biological scales
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2023'
...
---
_id: '12897'
abstract:
- lang: eng
text: "Inverse design problems in fabrication-aware shape optimization are typically
solved on discrete representations such as polygonal meshes. This thesis argues
that there are benefits to treating these problems in the same domain as human
designers, namely, the parametric one. One reason is that discretizing a parametric
model usually removes the capability of making further manual changes to the design,
because the human intent is captured by the shape parameters. Beyond this, knowledge
about a design problem can sometimes reveal a structure that is present in a smooth
representation, but is fundamentally altered by discretizing. In this case, working
in the parametric domain may even simplify the optimization task. We present two
lines of research that explore both of these aspects of fabrication-aware shape
optimization on parametric representations.\r\n\r\nThe first project studies the
design of plane elastic curves and Kirchhoff rods, which are common mathematical
models for describing the deformation of thin elastic rods such as beams, ribbons,
cables, and hair. Our main contribution is a characterization of all curved shapes
that can be attained by bending and twisting elastic rods having a stiffness that
is allowed to vary across the length. Elements like these can be manufactured
using digital fabrication devices such as 3d printers and digital cutters, and
have applications in free-form architecture and soft robotics.\r\n\r\nWe show
that the family of curved shapes that can be produced this way admits geometric
description that is concise and computationally convenient. In the case of plane
curves, the geometric description is intuitive enough to allow a designer to determine
whether a curved shape is physically achievable by visual inspection alone. We
also present shape optimization algorithms that convert a user-defined curve in
the plane or in three dimensions into the geometry of an elastic rod that will
naturally deform to follow this curve when its endpoints are attached to a support
structure. Implemented in an interactive software design tool, the rod geometry
is generated in real time as the user edits a curve and enables fast prototyping.
\r\n\r\nThe second project tackles the problem of general-purpose shape optimization
on CAD models using a novel variant of the extended finite element method (XFEM).
Our goal is the decoupling between the simulation mesh and the CAD model, so no
geometry-dependent meshing or remeshing needs to be performed when the CAD parameters
change during optimization. This is achieved by discretizing the embedding space
of the CAD model, and using a new high-accuracy numerical integration method to
enable XFEM on free-form elements bounded by the parametric surface patches of
the model. Our simulation is differentiable from the CAD parameters to the simulation
output, which enables us to use off-the-shelf gradient-based optimization procedures.
The result is a method that fits seamlessly into the CAD workflow because it works
on the same representation as the designer, enabling the alternation of manual
editing and fabrication-aware optimization at will."
acknowledged_ssus:
- _id: M-Shop
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Christian
full_name: Hafner, Christian
id: 400429CC-F248-11E8-B48F-1D18A9856A87
last_name: Hafner
citation:
ama: 'Hafner C. Inverse shape design with parametric representations: Kirchhoff
Rods and parametric surface models. 2023. doi:10.15479/at:ista:12897'
apa: 'Hafner, C. (2023). Inverse shape design with parametric representations:
Kirchhoff Rods and parametric surface models. Institute of Science and Technology
Austria. https://doi.org/10.15479/at:ista:12897'
chicago: 'Hafner, Christian. “Inverse Shape Design with Parametric Representations:
Kirchhoff Rods and Parametric Surface Models.” Institute of Science and Technology
Austria, 2023. https://doi.org/10.15479/at:ista:12897.'
ieee: 'C. Hafner, “Inverse shape design with parametric representations: Kirchhoff
Rods and parametric surface models,” Institute of Science and Technology Austria,
2023.'
ista: 'Hafner C. 2023. Inverse shape design with parametric representations: Kirchhoff
Rods and parametric surface models. Institute of Science and Technology Austria.'
mla: 'Hafner, Christian. Inverse Shape Design with Parametric Representations:
Kirchhoff Rods and Parametric Surface Models. Institute of Science and Technology
Austria, 2023, doi:10.15479/at:ista:12897.'
short: 'C. Hafner, Inverse Shape Design with Parametric Representations: Kirchhoff
Rods and Parametric Surface Models, Institute of Science and Technology Austria,
2023.'
corr_author: '1'
date_created: 2023-05-05T10:40:14Z
date_published: 2023-05-05T00:00:00Z
date_updated: 2025-04-15T07:16:15Z
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full_name: Bickel, Bernd
id: 49876194-F248-11E8-B48F-1D18A9856A87
last_name: Bickel
orcid: 0000-0001-6511-9385
title: 'Inverse shape design with parametric representations: Kirchhoff Rods and parametric
surface models'
type: dissertation
user_id: 400429CC-F248-11E8-B48F-1D18A9856A87
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...
---
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text: "The Kirchhoff rod model describes the bending and twisting of slender elastic
rods in three dimensions, and has been widely studied to enable the prediction
of how a rod will deform, given its geometry and boundary conditions. In this
work, we study a number of inverse problems with the goal of computing the geometry
of a straight rod that will automatically deform to match a curved target shape
after attaching its endpoints to a support structure. Our solution lets us finely
control the static equilibrium state of a rod by varying the cross-sectional profiles
along its length.\r\nWe also show that the set of physically realizable equilibrium
states admits a concise geometric description in terms of linear line complexes,
which leads to very efficient computational design algorithms. Implemented in
an interactive software tool, they allow us to convert three-dimensional hand-drawn
spline curves to elastic rods, and give feedback about the feasibility and practicality
of a design in real time. We demonstrate the efficacy of our method by designing
and manufacturing several physical prototypes with applications to interior design
and soft robotics."
acknowledged_ssus:
- _id: M-Shop
acknowledgement: We thank the anonymous reviewers for their generous feedback, and
Julian Fischer for his help in proving Proposition 1. This project has received
funding from the European Research Council (ERC) under the European Union’s Horizon
2020 research and innovation programme (grant agreement No. 715767).
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full_name: Hafner, Christian
id: 400429CC-F248-11E8-B48F-1D18A9856A87
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- first_name: Bernd
full_name: Bickel, Bernd
id: 49876194-F248-11E8-B48F-1D18A9856A87
last_name: Bickel
orcid: 0000-0001-6511-9385
citation:
ama: Hafner C, Bickel B. The design space of Kirchhoff rods. ACM Transactions
on Graphics. 2023;42(5). doi:10.1145/3606033
apa: Hafner, C., & Bickel, B. (2023). The design space of Kirchhoff rods. ACM
Transactions on Graphics. Association for Computing Machinery. https://doi.org/10.1145/3606033
chicago: Hafner, Christian, and Bernd Bickel. “The Design Space of Kirchhoff Rods.”
ACM Transactions on Graphics. Association for Computing Machinery, 2023.
https://doi.org/10.1145/3606033.
ieee: C. Hafner and B. Bickel, “The design space of Kirchhoff rods,” ACM Transactions
on Graphics, vol. 42, no. 5. Association for Computing Machinery, 2023.
ista: Hafner C, Bickel B. 2023. The design space of Kirchhoff rods. ACM Transactions
on Graphics. 42(5), 171.
mla: Hafner, Christian, and Bernd Bickel. “The Design Space of Kirchhoff Rods.”
ACM Transactions on Graphics, vol. 42, no. 5, 171, Association for Computing
Machinery, 2023, doi:10.1145/3606033.
short: C. Hafner, B. Bickel, ACM Transactions on Graphics 42 (2023).
corr_author: '1'
date_created: 2023-07-04T07:41:30Z
date_published: 2023-09-20T00:00:00Z
date_updated: 2026-08-31T22:30:05Z
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...
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abstract:
- lang: eng
text: "Understanding the mechanisms of learning and memory formation has always
been one of\r\nthe main goals in neuroscience. Already Pavlov (1927) in his early
days has used his classic\r\nconditioning experiments to study the neural mechanisms
governing behavioral adaptation.\r\nWhat was not known back then was that the
part of the brain that is largely responsible for\r\nthis type of associative
learning is the cerebellum.\r\nSince then, plenty of theories on cerebellar learning
have emerged. Despite their differences,\r\none thing they all have in common
is that learning relies on synaptic and intrinsic plasticity.\r\nThe goal of my
PhD project was to unravel the molecular mechanisms underlying synaptic\r\nplasticity
in two synapses that have been shown to be implicated in motor learning, in an\r\neffort
to understand how learning and memory formation are processed in the cerebellum.\r\nOne
of the earliest and most well-known cerebellar theories postulates that motor
learning\r\nlargely depends on long-term depression at the parallel fiber-Purkinje
cell (PC-PC) synapse.\r\nHowever, the discovery of other types of plasticity in
the cerebellar circuitry, like long-term\r\npotentiation (LTP) at the PC-PC synapse,
potentiation of molecular layer interneurons (MLIs),\r\nand plasticity transfer
from the cortex to the cerebellar/ vestibular nuclei has increased the\r\npopularity
of the idea that multiple sites of plasticity might be involved in learning.\r\nStill
a lot remains unknown about the molecular mechanisms responsible for these types
of\r\nplasticity and whether they occur during physiological learning.\r\nIn the
first part of this thesis we have analyzed the variation and nanodistribution
of voltagegated calcium channels (VGCCs) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic
acid\r\ntype glutamate receptors (AMPARs) on the parallel fiber-Purkinje cell
synapse after vestibuloocular reflex phase reversal adaptation, a behavior that
has been suggested to rely on PF-PC\r\nLTP. We have found that on the last day
of adaptation there is no learning trace in form of\r\nVGCCs nor AMPARs variation
at the PF-PC synapse, but instead a decrease in the number of\r\nPF-PC synapses.
These data seem to support the view that learning is only stored in the\r\ncerebellar
cortex in an initial learning phase, being transferred later to the vestibular
nuclei.\r\nNext, we have studied the role of MLIs in motor learning using a relatively
simple and well characterized behavioral paradigm – horizontal optokinetic reflex
(HOKR) adaptation. We\r\nhave found behavior-induced MLI potentiation in form
of release probability increase that\r\ncould be explained by the increase of
VGCCs at the presynaptic side. Our results strengthen\r\nthe idea of distributed
cerebellar plasticity contributing to learning and provide a novel\r\nmechanism
for release probability increase. "
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
- _id: PreCl
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Catarina
full_name: Alcarva, Catarina
id: 3A96634C-F248-11E8-B48F-1D18A9856A87
last_name: Alcarva
citation:
ama: 'Alcarva C. Plasticity in the cerebellum: What molecular mechanisms are behind
physiological learning. 2023. doi:10.15479/at:ista:12809'
apa: 'Alcarva, C. (2023). Plasticity in the cerebellum: What molecular mechanisms
are behind physiological learning. Institute of Science and Technology Austria.
https://doi.org/10.15479/at:ista:12809'
chicago: 'Alcarva, Catarina. “Plasticity in the Cerebellum: What Molecular Mechanisms
Are behind Physiological Learning.” Institute of Science and Technology Austria,
2023. https://doi.org/10.15479/at:ista:12809.'
ieee: 'C. Alcarva, “Plasticity in the cerebellum: What molecular mechanisms are
behind physiological learning,” Institute of Science and Technology Austria, 2023.'
ista: 'Alcarva C. 2023. Plasticity in the cerebellum: What molecular mechanisms
are behind physiological learning. Institute of Science and Technology Austria.'
mla: 'Alcarva, Catarina. Plasticity in the Cerebellum: What Molecular Mechanisms
Are behind Physiological Learning. Institute of Science and Technology Austria,
2023, doi:10.15479/at:ista:12809.'
short: 'C. Alcarva, Plasticity in the Cerebellum: What Molecular Mechanisms Are
behind Physiological Learning, Institute of Science and Technology Austria, 2023.'
corr_author: '1'
date_created: 2023-04-06T07:54:09Z
date_published: 2023-04-06T00:00:00Z
date_updated: 2026-04-07T13:53:28Z
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supervisor:
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full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
title: 'Plasticity in the cerebellum: What molecular mechanisms are behind physiological
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type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
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...