[{"abstract":[{"lang":"eng","text":"Cell polarity is a fundamental feature of all multicellular organisms. In plants, prominent cell polarity markers are PIN auxin transporters crucial for plant development. To identify novel components involved in cell polarity establishment and maintenance, we carried out a forward genetic screening with PIN2:PIN1-HA;pin2 Arabidopsis plants, which ectopically express predominantly basally localized PIN1 in the root epidermal cells leading to agravitropic root growth. From the screen, we identified the regulator of PIN polarity 12 (repp12) mutation, which restored gravitropic root growth and caused PIN1-HA polarity switch from basal to apical side of root epidermal cells. Complementation experiments established the repp12 causative mutation as an amino acid substitution in Aminophospholipid ATPase3 (ALA3), a phospholipid flippase with predicted function in vesicle formation. ala3 T-DNA mutants show defects in many auxin-regulated processes, in asymmetric auxin distribution and in PIN trafficking. Analysis of quintuple and sextuple mutants confirmed a crucial role of ALA proteins in regulating plant development and in PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with GNOM and BIG3 ARF GEFs. Taken together, our results identified ALA3 flippase as an important interactor and regulator of ARF GEF functioning in PIN polarity, trafficking and auxin-mediated development."}],"publication":"The Plant Cell","volume":32,"ec_funded":1,"title":"Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters","acknowledged_ssus":[{"_id":"Bio"}],"project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"No","page":"1644-1664","external_id":{"pmid":["32193204"],"isi":["000545741500030"]},"date_created":"2020-03-28T07:39:22Z","doi":"10.1105/tpc.19.00869","_id":"7619","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"main_file_link":[{"url":"https://doi.org/10.1105/tpc.19.00869","open_access":"1"}],"scopus_import":"1","citation":{"ista":"Zhang X, Adamowski M, Marhavá P, Tan S, Zhang Y, Rodriguez Solovey L, Zwiewka M, Pukyšová V, Sánchez AS, Raxwal VK, Hardtke CS, Nodzynski T, Friml J. 2020. Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters. The Plant Cell. 32(5), 1644–1664.","apa":"Zhang, X., Adamowski, M., Marhavá, P., Tan, S., Zhang, Y., Rodriguez Solovey, L., … Friml, J. (2020). Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters. <i>The Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.19.00869\">https://doi.org/10.1105/tpc.19.00869</a>","short":"X. Zhang, M. Adamowski, P. Marhavá, S. Tan, Y. Zhang, L. Rodriguez Solovey, M. Zwiewka, V. Pukyšová, A.S. Sánchez, V.K. Raxwal, C.S. Hardtke, T. Nodzynski, J. Friml, The Plant Cell 32 (2020) 1644–1664.","mla":"Zhang, Xixi, et al. “Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.” <i>The Plant Cell</i>, vol. 32, no. 5, American Society of Plant Biologists, 2020, pp. 1644–64, doi:<a href=\"https://doi.org/10.1105/tpc.19.00869\">10.1105/tpc.19.00869</a>.","ieee":"X. Zhang <i>et al.</i>, “Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters,” <i>The Plant Cell</i>, vol. 32, no. 5. American Society of Plant Biologists, pp. 1644–1664, 2020.","ama":"Zhang X, Adamowski M, Marhavá P, et al. Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters. <i>The Plant Cell</i>. 2020;32(5):1644-1664. doi:<a href=\"https://doi.org/10.1105/tpc.19.00869\">10.1105/tpc.19.00869</a>","chicago":"Zhang, Xixi, Maciek Adamowski, Petra Marhavá, Shutang Tan, Yuzhou Zhang, Lesia Rodriguez Solovey, Marta Zwiewka, et al. “Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.” <i>The Plant Cell</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1105/tpc.19.00869\">https://doi.org/10.1105/tpc.19.00869</a>."},"month":"05","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","issue":"5","quality_controlled":"1","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"oa":1,"oa_version":"Published Version","ddc":["580"],"date_published":"2020-05-01T00:00:00Z","corr_author":"1","author":[{"orcid":"0000-0001-7048-4627","full_name":"Zhang, Xixi","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","first_name":"Xixi","last_name":"Zhang"},{"last_name":"Adamowski","first_name":"Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","full_name":"Adamowski, Maciek","orcid":"0000-0001-6463-5257"},{"id":"44E59624-F248-11E8-B48F-1D18A9856A87","full_name":"Marhavá, Petra","last_name":"Marhavá","first_name":"Petra"},{"id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285","last_name":"Tan","first_name":"Shutang"},{"orcid":"0000-0003-2627-6956","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","full_name":"Zhang, Yuzhou","last_name":"Zhang","first_name":"Yuzhou"},{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","full_name":"Rodriguez Solovey, Lesia","orcid":"0000-0002-7244-7237","last_name":"Rodriguez Solovey","first_name":"Lesia"},{"last_name":"Zwiewka","first_name":"Marta","full_name":"Zwiewka, Marta"},{"full_name":"Pukyšová, Vendula","last_name":"Pukyšová","first_name":"Vendula"},{"full_name":"Sánchez, Adrià Sans","last_name":"Sánchez","first_name":"Adrià Sans"},{"full_name":"Raxwal, Vivek Kumar","first_name":"Vivek Kumar","last_name":"Raxwal"},{"full_name":"Hardtke, Christian S.","first_name":"Christian S.","last_name":"Hardtke"},{"full_name":"Nodzynski, Tomasz","first_name":"Tomasz","last_name":"Nodzynski"},{"last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"}],"isi":1,"intvolume":"        32","status":"public","publication_status":"published","pmid":1,"article_type":"original","publisher":"American Society of Plant Biologists","date_updated":"2026-06-18T19:24:19Z","day":"01","year":"2020"},{"day":"08","year":"2020","article_type":"letter_note","publisher":"American Society of Plant Biologists","date_updated":"2026-06-18T19:25:52Z","pmid":1,"publication_status":"published","status":"public","isi":1,"intvolume":"       183","author":[{"last_name":"Han","first_name":"Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87","full_name":"Han, Huibin"},{"id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA","full_name":"Rakusova, Hana","last_name":"Rakusova","first_name":"Hana"},{"orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","first_name":"Inge","last_name":"Verstraeten"},{"last_name":"Zhang","first_name":"Yuzhou","orcid":"0000-0003-2627-6956","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","full_name":"Zhang, Yuzhou"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"}],"date_published":"2020-05-08T00:00:00Z","ddc":["580"],"corr_author":"1","department":[{"_id":"JiFr"}],"oa_version":"Published Version","oa":1,"quality_controlled":"1","language":[{"iso":"eng"}],"issue":"5","citation":{"apa":"Han, H., Rakusova, H., Verstraeten, I., Zhang, Y., &#38; Friml, J. (2020). SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.20.00212\">https://doi.org/10.1104/pp.20.00212</a>","ista":"Han H, Rakusova H, Verstraeten I, Zhang Y, Friml J. 2020. SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism. Plant Physiology. 183(5), 37–40.","chicago":"Han, Huibin, Hana Rakusova, Inge Verstraeten, Yuzhou Zhang, and Jiří Friml. “SCF TIR1/AFB Auxin Signaling for Bending Termination during Shoot Gravitropism.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1104/pp.20.00212\">https://doi.org/10.1104/pp.20.00212</a>.","ama":"Han H, Rakusova H, Verstraeten I, Zhang Y, Friml J. SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism. <i>Plant Physiology</i>. 2020;183(5):37-40. doi:<a href=\"https://doi.org/10.1104/pp.20.00212\">10.1104/pp.20.00212</a>","mla":"Han, Huibin, et al. “SCF TIR1/AFB Auxin Signaling for Bending Termination during Shoot Gravitropism.” <i>Plant Physiology</i>, vol. 183, no. 5, American Society of Plant Biologists, 2020, pp. 37–40, doi:<a href=\"https://doi.org/10.1104/pp.20.00212\">10.1104/pp.20.00212</a>.","ieee":"H. Han, H. Rakusova, I. Verstraeten, Y. Zhang, and J. Friml, “SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism,” <i>Plant Physiology</i>, vol. 183, no. 5. American Society of Plant Biologists, pp. 37–40, 2020.","short":"H. Han, H. Rakusova, I. Verstraeten, Y. Zhang, J. Friml, Plant Physiology 183 (2020) 37–40."},"scopus_import":"1","type":"journal_article","month":"05","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"7643","publication_identifier":{"eissn":["1532-2548"],"issn":["0032-0889"]},"related_material":{"record":[{"id":"8589","relation":"dissertation_contains","status":"public"}]},"doi":"10.1104/pp.20.00212","date_created":"2020-04-06T10:06:40Z","main_file_link":[{"url":"https://doi.org/10.1104/pp.20.00212","open_access":"1"}],"acknowledgement":"This work was supported by the European Research Council under the European Union’s Horizon 2020 research and innovation Programme (ERC grant agreement number 742985), and the Austrian Science Fund (FWF, grant number I 3630-B25) to JF. HH is supported by the China Scholarship Council (CSC scholarship). ","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"}],"external_id":{"isi":["000536641800018"],"pmid":["32107280"]},"page":"37-40","article_processing_charge":"No","title":"SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism","ec_funded":1,"volume":183,"publication":"Plant Physiology"},{"ddc":["580"],"date_published":"2020-07-06T00:00:00Z","author":[{"first_name":"E","last_name":"Lee","full_name":"Lee, E"},{"first_name":"B","last_name":"Vila Nova Santana","full_name":"Vila Nova Santana, B"},{"full_name":"Samuels, E","last_name":"Samuels","first_name":"E"},{"last_name":"Benitez-Fuente","first_name":"F","full_name":"Benitez-Fuente, F"},{"last_name":"Corsi","first_name":"E","full_name":"Corsi, E"},{"first_name":"MA","last_name":"Botella","full_name":"Botella, MA"},{"last_name":"Perez-Sancho","first_name":"J","full_name":"Perez-Sancho, J"},{"full_name":"Vanneste, S","first_name":"S","last_name":"Vanneste"},{"first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"full_name":"Macho, A","last_name":"Macho","first_name":"A"},{"full_name":"Alves Azevedo, A","last_name":"Alves Azevedo","first_name":"A"},{"first_name":"A","last_name":"Rosado","full_name":"Rosado, A"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"intvolume":"        71","has_accepted_license":"1","status":"public","pmid":1,"publication_status":"published","publisher":"Oxford University Press","article_type":"original","date_updated":"2024-10-21T06:02:26Z","day":"06","year":"2020","file":[{"access_level":"open_access","checksum":"b06aaaa93dc41896da805fe4b75cf3a1","relation":"main_file","date_created":"2020-10-06T07:41:35Z","file_size":1916031,"content_type":"application/pdf","date_updated":"2020-10-06T07:41:35Z","creator":"dernst","file_id":"8613","file_name":"2020_JourExperimBotany_Lee.pdf","success":1}],"file_date_updated":"2020-10-06T07:41:35Z","abstract":[{"text":"In plant cells, environmental stressors promote changes in connectivity between the cortical ER and the PM. Although this process is tightly regulated in space and time, the molecular signals and structural components mediating these changes in inter-organelle communication are only starting to be characterized. In this report, we confirm the presence of a putative tethering complex containing the synaptotagmins 1 and 5 (SYT1 and SYT5) and the Ca2+ and lipid binding protein 1 (CLB1/SYT7). This complex is enriched at ER-PM contact sites (EPCS), have slow responses to changes in extracellular Ca2+, and display severe cytoskeleton-dependent rearrangements in response to the trivalent lanthanum (La3+) and gadolinium (Gd3+) rare earth elements (REEs). Although REEs are generally used as non-selective cation channel blockers at the PM, here we show that the slow internalization of REEs into the cytosol underlies the activation of the Ca2+/Calmodulin intracellular signaling, the accumulation of phosphatidylinositol-4-phosphate (PI4P) at the PM, and the cytoskeleton-dependent rearrangement of the SYT1/SYT5 EPCS complexes. We propose that the observed EPCS rearrangements act as a slow adaptive response to sustained stress conditions, and that this process involves the accumulation of stress-specific phosphoinositides species at the PM.","lang":"eng"}],"publication":"Journal of Experimental Botany","volume":71,"title":"Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis","article_processing_charge":"No","page":"3986–3998","external_id":{"isi":["000553125400007"],"pmid":["32179893"]},"date_created":"2020-04-06T10:57:08Z","doi":"10.1093/jxb/eraa138","publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"_id":"7646","scopus_import":"1","citation":{"apa":"Lee, E., Vila Nova Santana, B., Samuels, E., Benitez-Fuente, F., Corsi, E., Botella, M., … Rosado, A. (2020). Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/eraa138\">https://doi.org/10.1093/jxb/eraa138</a>","ista":"Lee E, Vila Nova Santana B, Samuels E, Benitez-Fuente F, Corsi E, Botella M, Perez-Sancho J, Vanneste S, Friml J, Macho A, Alves Azevedo A, Rosado A. 2020. Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. Journal of Experimental Botany. 71(14), 3986–3998.","chicago":"Lee, E, B Vila Nova Santana, E Samuels, F Benitez-Fuente, E Corsi, MA Botella, J Perez-Sancho, et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and PI4P-Associated Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2020. <a href=\"https://doi.org/10.1093/jxb/eraa138\">https://doi.org/10.1093/jxb/eraa138</a>.","ama":"Lee E, Vila Nova Santana B, Samuels E, et al. Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. <i>Journal of Experimental Botany</i>. 2020;71(14):3986–3998. doi:<a href=\"https://doi.org/10.1093/jxb/eraa138\">10.1093/jxb/eraa138</a>","ieee":"E. Lee <i>et al.</i>, “Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis,” <i>Journal of Experimental Botany</i>, vol. 71, no. 14. Oxford University Press, pp. 3986–3998, 2020.","mla":"Lee, E., et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and PI4P-Associated Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.” <i>Journal of Experimental Botany</i>, vol. 71, no. 14, Oxford University Press, 2020, pp. 3986–3998, doi:<a href=\"https://doi.org/10.1093/jxb/eraa138\">10.1093/jxb/eraa138</a>.","short":"E. Lee, B. Vila Nova Santana, E. Samuels, F. Benitez-Fuente, E. Corsi, M. Botella, J. Perez-Sancho, S. Vanneste, J. Friml, A. Macho, A. Alves Azevedo, A. Rosado, Journal of Experimental Botany 71 (2020) 3986–3998."},"month":"07","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","type":"journal_article","issue":"14","language":[{"iso":"eng"}],"quality_controlled":"1","department":[{"_id":"JiFr"}],"oa_version":"Published Version","oa":1},{"type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"06","citation":{"ama":"Xue H, Zhang Y, Xiao G. Neo-gibberellin signaling: Guiding the next generation of the green revolution. <i>Trends in Plant Science</i>. 2020;25(6):520-522. doi:<a href=\"https://doi.org/10.1016/j.tplants.2020.04.001\">10.1016/j.tplants.2020.04.001</a>","chicago":"Xue, Huidan, Yuzhou Zhang, and Guanghui Xiao. “Neo-Gibberellin Signaling: Guiding the next Generation of the Green Revolution.” <i>Trends in Plant Science</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.tplants.2020.04.001\">https://doi.org/10.1016/j.tplants.2020.04.001</a>.","short":"H. Xue, Y. Zhang, G. Xiao, Trends in Plant Science 25 (2020) 520–522.","ieee":"H. Xue, Y. Zhang, and G. Xiao, “Neo-gibberellin signaling: Guiding the next generation of the green revolution,” <i>Trends in Plant Science</i>, vol. 25, no. 6. Elsevier, pp. 520–522, 2020.","mla":"Xue, Huidan, et al. “Neo-Gibberellin Signaling: Guiding the next Generation of the Green Revolution.” <i>Trends in Plant Science</i>, vol. 25, no. 6, Elsevier, 2020, pp. 520–22, doi:<a href=\"https://doi.org/10.1016/j.tplants.2020.04.001\">10.1016/j.tplants.2020.04.001</a>.","apa":"Xue, H., Zhang, Y., &#38; Xiao, G. (2020). Neo-gibberellin signaling: Guiding the next generation of the green revolution. <i>Trends in Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.tplants.2020.04.001\">https://doi.org/10.1016/j.tplants.2020.04.001</a>","ista":"Xue H, Zhang Y, Xiao G. 2020. Neo-gibberellin signaling: Guiding the next generation of the green revolution. Trends in Plant Science. 25(6), 520–522."},"scopus_import":"1","_id":"7686","publication_identifier":{"issn":["1360-1385"]},"doi":"10.1016/j.tplants.2020.04.001","date_created":"2020-04-26T22:00:46Z","oa_version":"None","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"6","title":"Neo-gibberellin signaling: Guiding the next generation of the green revolution","volume":25,"publication":"Trends in Plant Science","abstract":[{"text":"The agricultural green revolution spectacularly enhanced crop yield and lodging resistance with modified DELLA-mediated gibberellin signaling. However, this was achieved at the expense of reduced nitrogen-use efficiency (NUE). Recently, Wu et al. revealed novel gibberellin signaling that provides a blueprint for improving tillering and NUE in Green Revolution varieties (GRVs). ","lang":"eng"}],"article_processing_charge":"No","page":"520-522","external_id":{"pmid":["32407691"],"isi":["000533518400003"]},"date_updated":"2025-06-25T10:59:39Z","publisher":"Elsevier","article_type":"original","publication_status":"published","pmid":1,"status":"public","year":"2020","day":"01","author":[{"full_name":"Xue, Huidan","last_name":"Xue","first_name":"Huidan"},{"first_name":"Yuzhou","last_name":"Zhang","full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956"},{"last_name":"Xiao","first_name":"Guanghui","full_name":"Xiao, Guanghui"}],"date_published":"2020-06-01T00:00:00Z","intvolume":"        25","isi":1},{"title":"High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits","abstract":[{"text":"The TPLATE complex (TPC) is a key endocytic adaptor protein complex in plants. TPC in Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and two plant-specific subunits, AtEH1/Pan1 and AtEH2/Pan1, although cytoplasmic proteins are not associated with the hexameric subcomplex in the cytoplasm. To investigate the dynamic assembly of the octameric TPC at the plasma membrane (PM), we performed state-of-the-art dual-color live cell imaging at physiological and lowered temperatures. Lowering the temperature slowed down endocytosis, thereby enhancing the temporal resolution of the differential recruitment of endocytic components. Under both normal and lowered temperature conditions, the core TPC subunit TPLATE and the AtEH/Pan1 proteins exhibited simultaneous recruitment at the PM. These results, together with co-localization analysis of different TPC subunits, allow us to conclude that TPC in plant cells is not recruited to the PM sequentially but as an octameric complex.","lang":"eng"}],"publication":"Plant Physiology","volume":183,"project":[{"_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants"}],"article_processing_charge":"No","page":"986-997","external_id":{"isi":["000550682000018"],"pmid":["32321842"]},"scopus_import":"1","citation":{"ama":"Wang J, Mylle E, Johnson AJ, et al. High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits. <i>Plant Physiology</i>. 2020;183(3):986-997. doi:<a href=\"https://doi.org/10.1104/pp.20.00178\">10.1104/pp.20.00178</a>","chicago":"Wang, J, E Mylle, Alexander J Johnson, N Besbrugge, G De Jaeger, Jiří Friml, R Pleskot, and D van Damme. “High Temporal Resolution Reveals Simultaneous Plasma Membrane Recruitment of TPLATE Complex Subunits.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1104/pp.20.00178\">https://doi.org/10.1104/pp.20.00178</a>.","short":"J. Wang, E. Mylle, A.J. Johnson, N. Besbrugge, G. De Jaeger, J. Friml, R. Pleskot, D. van Damme, Plant Physiology 183 (2020) 986–997.","mla":"Wang, J., et al. “High Temporal Resolution Reveals Simultaneous Plasma Membrane Recruitment of TPLATE Complex Subunits.” <i>Plant Physiology</i>, vol. 183, no. 3, American Society of Plant Biologists, 2020, pp. 986–97, doi:<a href=\"https://doi.org/10.1104/pp.20.00178\">10.1104/pp.20.00178</a>.","ieee":"J. Wang <i>et al.</i>, “High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits,” <i>Plant Physiology</i>, vol. 183, no. 3. American Society of Plant Biologists, pp. 986–997, 2020.","apa":"Wang, J., Mylle, E., Johnson, A. J., Besbrugge, N., De Jaeger, G., Friml, J., … van Damme, D. (2020). High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.20.00178\">https://doi.org/10.1104/pp.20.00178</a>","ista":"Wang J, Mylle E, Johnson AJ, Besbrugge N, De Jaeger G, Friml J, Pleskot R, van Damme D. 2020. High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits. Plant Physiology. 183(3), 986–997."},"month":"07","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","type":"journal_article","doi":"10.1104/pp.20.00178","date_created":"2020-04-29T15:23:00Z","publication_identifier":{"eissn":["1532-2548"],"issn":["0032-0889"]},"_id":"7695","main_file_link":[{"url":"https://doi.org/10.1101/2020.02.13.948109","open_access":"1"}],"department":[{"_id":"JiFr"}],"oa":1,"oa_version":"Preprint","issue":"3","language":[{"iso":"eng"}],"quality_controlled":"1","author":[{"full_name":"Wang, J","last_name":"Wang","first_name":"J"},{"first_name":"E","last_name":"Mylle","full_name":"Mylle, E"},{"orcid":"0000-0002-2739-8843","full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","last_name":"Johnson"},{"last_name":"Besbrugge","first_name":"N","full_name":"Besbrugge, N"},{"full_name":"De Jaeger, G","last_name":"De Jaeger","first_name":"G"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml"},{"full_name":"Pleskot, R","last_name":"Pleskot","first_name":"R"},{"full_name":"van Damme, D","last_name":"van Damme","first_name":"D"}],"date_published":"2020-07-01T00:00:00Z","isi":1,"intvolume":"       183","publisher":"American Society of Plant Biologists","article_type":"original","date_updated":"2025-04-15T07:32:09Z","status":"public","pmid":1,"publication_status":"published","day":"01","year":"2020"},{"date_updated":"2025-04-14T07:45:03Z","publisher":"Wiley","article_type":"original","publication_status":"published","pmid":1,"status":"public","file":[{"file_id":"8799","file_name":"2020_09_NewPhytologist_Zhang.pdf","success":1,"access_level":"open_access","checksum":"8e8150dbbba8cb65b72f81d1f0864b8b","date_created":"2020-11-24T12:19:38Z","relation":"main_file","content_type":"application/pdf","date_updated":"2020-11-24T12:19:38Z","file_size":3643395,"creator":"dernst"}],"year":"2020","day":"01","author":[{"id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","full_name":"Zhang, Yuzhou","orcid":"0000-0003-2627-6956","last_name":"Zhang","first_name":"Yuzhou"},{"last_name":"Hartinger","first_name":"Corinna","orcid":"0000-0003-1618-2737","id":"AEFB2266-8ABF-11EA-AA39-812C3623CBE4","full_name":"Hartinger, Corinna"},{"full_name":"Wang, Xiaojuan","first_name":"Xiaojuan","last_name":"Wang"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"}],"corr_author":"1","date_published":"2020-09-01T00:00:00Z","ddc":["580"],"has_accepted_license":"1","intvolume":"       227","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"type":"journal_article","month":"09","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"short":"Y. Zhang, C. Hartinger, X. Wang, J. Friml, New Phytologist 227 (2020) 1406–1416.","mla":"Zhang, Yuzhou, et al. “Directional Auxin Fluxes in Plants by Intramolecular Domain‐domain Co‐evolution of PIN Auxin Transporters.” <i>New Phytologist</i>, vol. 227, no. 5, Wiley, 2020, pp. 1406–16, doi:<a href=\"https://doi.org/10.1111/nph.16629\">10.1111/nph.16629</a>.","ieee":"Y. Zhang, C. Hartinger, X. Wang, and J. Friml, “Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters,” <i>New Phytologist</i>, vol. 227, no. 5. Wiley, pp. 1406–1416, 2020.","ama":"Zhang Y, Hartinger C, Wang X, Friml J. Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters. <i>New Phytologist</i>. 2020;227(5):1406-1416. doi:<a href=\"https://doi.org/10.1111/nph.16629\">10.1111/nph.16629</a>","chicago":"Zhang, Yuzhou, Corinna Hartinger, Xiaojuan Wang, and Jiří Friml. “Directional Auxin Fluxes in Plants by Intramolecular Domain‐domain Co‐evolution of PIN Auxin Transporters.” <i>New Phytologist</i>. Wiley, 2020. <a href=\"https://doi.org/10.1111/nph.16629\">https://doi.org/10.1111/nph.16629</a>.","ista":"Zhang Y, Hartinger C, Wang X, Friml J. 2020. Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters. New Phytologist. 227(5), 1406–1416.","apa":"Zhang, Y., Hartinger, C., Wang, X., &#38; Friml, J. (2020). Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16629\">https://doi.org/10.1111/nph.16629</a>"},"scopus_import":"1","_id":"7697","publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"date_created":"2020-04-30T08:43:29Z","doi":"10.1111/nph.16629","oa_version":"Published Version","oa":1,"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"5","title":"Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters","volume":227,"ec_funded":1,"abstract":[{"lang":"eng","text":"* Morphogenesis and adaptive tropic growth in plants depend on gradients of the phytohormone auxin, mediated by the membrane‐based PIN‐FORMED (PIN) auxin transporters. PINs localize to a particular side of the plasma membrane (PM) or to the endoplasmic reticulum (ER) to directionally transport auxin and maintain intercellular and intracellular auxin homeostasis, respectively. However, the molecular cues that confer their diverse cellular localizations remain largely unknown.\r\n* In this study, we systematically swapped the domains between ER‐ and PM‐localized PIN proteins, as well as between apical and basal PM‐localized PINs from Arabidopsis thaliana , to shed light on why PIN family members with similar topological structures reside at different membrane compartments within cells.\r\n* Our results show that not only do the N‐ and C‐terminal transmembrane domains (TMDs) and central hydrophilic loop contribute to their differential subcellular localizations and cellular polarity, but that the pairwise‐matched N‐ and C‐terminal TMDs resulting from intramolecular domain–domain coevolution are also crucial for their divergent patterns of localization.\r\n* These findings illustrate the complexity of the evolutionary path of PIN proteins in acquiring their plethora of developmental functions and adaptive growth in plants."}],"publication":"New Phytologist","file_date_updated":"2020-11-24T12:19:38Z","article_processing_charge":"Yes (via OA deal)","page":"1406-1416","external_id":{"pmid":["32350870"],"isi":["000534092400001"]},"project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"},{"grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF"},{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"}]},{"abstract":[{"text":"Hormonal signalling in animals often involves direct transcription factor-hormone interactions that modulate gene expression. In contrast, plant hormone signalling is most commonly based on de-repression via the degradation of transcriptional repressors. Recently, we uncovered a non-canonical signalling mechanism for the plant hormone auxin whereby auxin directly affects the activity of the atypical auxin response factor (ARF), ETTIN towards target genes without the requirement for protein degradation. Here we show that ETTIN directly binds auxin, leading to dissociation from co-repressor proteins of the TOPLESS/TOPLESS-RELATED family followed by histone acetylation and induction of gene expression. This mechanism is reminiscent of animal hormone signalling as it affects the activity towards regulation of target genes and provides the first example of a DNA-bound hormone receptor in plants. Whilst auxin affects canonical ARFs indirectly by facilitating degradation of Aux/IAA repressors, direct ETTIN-auxin interactions allow switching between repressive and de-repressive chromatin states in an instantly-reversible manner.","lang":"eng"}],"publication":"eLife","volume":9,"file_date_updated":"2020-07-14T12:48:03Z","article_number":"e51787","title":"Direct ETTIN-auxin interaction controls chromatin states in gynoecium development","article_processing_charge":"No","external_id":{"isi":["000527752200001"],"pmid":["32267233"]},"doi":"10.7554/elife.51787","date_created":"2020-05-04T08:50:47Z","_id":"7793","publication_identifier":{"issn":["2050-084X"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"04","type":"journal_article","scopus_import":"1","citation":{"ieee":"A. Kuhn <i>et al.</i>, “Direct ETTIN-auxin interaction controls chromatin states in gynoecium development,” <i>eLife</i>, vol. 9. eLife Sciences Publications, 2020.","mla":"Kuhn, André, et al. “Direct ETTIN-Auxin Interaction Controls Chromatin States in Gynoecium Development.” <i>ELife</i>, vol. 9, e51787, eLife Sciences Publications, 2020, doi:<a href=\"https://doi.org/10.7554/elife.51787\">10.7554/elife.51787</a>.","short":"A. Kuhn, S. Ramans Harborough, H.M. McLaughlin, B. Natarajan, I. Verstraeten, J. Friml, S. Kepinski, L. Østergaard, ELife 9 (2020).","chicago":"Kuhn, André, Sigurd Ramans Harborough, Heather M McLaughlin, Bhavani Natarajan, Inge Verstraeten, Jiří Friml, Stefan Kepinski, and Lars Østergaard. “Direct ETTIN-Auxin Interaction Controls Chromatin States in Gynoecium Development.” <i>ELife</i>. eLife Sciences Publications, 2020. <a href=\"https://doi.org/10.7554/elife.51787\">https://doi.org/10.7554/elife.51787</a>.","ama":"Kuhn A, Ramans Harborough S, McLaughlin HM, et al. Direct ETTIN-auxin interaction controls chromatin states in gynoecium development. <i>eLife</i>. 2020;9. doi:<a href=\"https://doi.org/10.7554/elife.51787\">10.7554/elife.51787</a>","ista":"Kuhn A, Ramans Harborough S, McLaughlin HM, Natarajan B, Verstraeten I, Friml J, Kepinski S, Østergaard L. 2020. Direct ETTIN-auxin interaction controls chromatin states in gynoecium development. eLife. 9, e51787.","apa":"Kuhn, A., Ramans Harborough, S., McLaughlin, H. M., Natarajan, B., Verstraeten, I., Friml, J., … Østergaard, L. (2020). Direct ETTIN-auxin interaction controls chromatin states in gynoecium development. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.51787\">https://doi.org/10.7554/elife.51787</a>"},"quality_controlled":"1","language":[{"iso":"eng"}],"oa_version":"Published Version","oa":1,"department":[{"_id":"JiFr"}],"date_published":"2020-04-08T00:00:00Z","ddc":["580"],"author":[{"full_name":"Kuhn, André","first_name":"André","last_name":"Kuhn"},{"last_name":"Ramans Harborough","first_name":"Sigurd","full_name":"Ramans Harborough, Sigurd"},{"full_name":"McLaughlin, Heather M","last_name":"McLaughlin","first_name":"Heather M"},{"first_name":"Bhavani","last_name":"Natarajan","full_name":"Natarajan, Bhavani"},{"last_name":"Verstraeten","first_name":"Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","full_name":"Verstraeten, Inge","orcid":"0000-0001-7241-2328"},{"first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"full_name":"Kepinski, Stefan","first_name":"Stefan","last_name":"Kepinski"},{"full_name":"Østergaard, Lars","first_name":"Lars","last_name":"Østergaard"}],"intvolume":"         9","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","status":"public","pmid":1,"publication_status":"published","date_updated":"2023-08-21T06:17:12Z","publisher":"eLife Sciences Publications","article_type":"original","year":"2020","day":"08","file":[{"file_id":"7794","file_name":"2020_eLife_Kuhn.pdf","access_level":"open_access","relation":"main_file","date_created":"2020-05-04T09:06:43Z","checksum":"15d740de1a741fdcc6ec128c48eed017","file_size":2893082,"content_type":"application/pdf","date_updated":"2020-07-14T12:48:03Z","creator":"dernst"}]},{"department":[{"_id":"JiFr"}],"oa_version":"Published Version","oa":1,"issue":"8","language":[{"iso":"eng"}],"quality_controlled":"1","scopus_import":"1","citation":{"ista":"Smith S, Zhu S, Joos L, Roberts I, Nikonorova N, Vu L, Stes E, Cho H, Larrieu A, Xuan W, Goodall B, van de Cotte B, Waite J, Rigal A, R Harborough S, Persiau G, Vanneste S, Kirschner G, Vandermarliere E, Martens L, Stahl Y, Audenaert D, Friml J, Felix G, Simon R, Bennett M, Bishopp A, De Jaeger G, Ljung K, Kepinski S, Robert S, Nemhauser J, Hwang I, Gevaert K, Beeckman T, De Smet I. 2020. The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. Molecular &#38; Cellular Proteomics. 19(8), 1248–1262.","apa":"Smith, S., Zhu, S., Joos, L., Roberts, I., Nikonorova, N., Vu, L., … De Smet, I. (2020). The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. <i>Molecular &#38; Cellular Proteomics</i>. American Society for Biochemistry and Molecular Biology. <a href=\"https://doi.org/10.1074/mcp.ra119.001826\">https://doi.org/10.1074/mcp.ra119.001826</a>","short":"S. Smith, S. Zhu, L. Joos, I. Roberts, N. Nikonorova, L. Vu, E. Stes, H. Cho, A. Larrieu, W. Xuan, B. Goodall, B. van de Cotte, J. Waite, A. Rigal, S. R Harborough, G. Persiau, S. Vanneste, G. Kirschner, E. Vandermarliere, L. Martens, Y. Stahl, D. Audenaert, J. Friml, G. Felix, R. Simon, M. Bennett, A. Bishopp, G. De Jaeger, K. Ljung, S. Kepinski, S. Robert, J. Nemhauser, I. Hwang, K. Gevaert, T. Beeckman, I. De Smet, Molecular &#38; Cellular Proteomics 19 (2020) 1248–1262.","mla":"Smith, S., et al. “The CEP5 Peptide Promotes Abiotic Stress Tolerance, as Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis.” <i>Molecular &#38; Cellular Proteomics</i>, vol. 19, no. 8, American Society for Biochemistry and Molecular Biology, 2020, pp. 1248–62, doi:<a href=\"https://doi.org/10.1074/mcp.ra119.001826\">10.1074/mcp.ra119.001826</a>.","ieee":"S. Smith <i>et al.</i>, “The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis,” <i>Molecular &#38; Cellular Proteomics</i>, vol. 19, no. 8. American Society for Biochemistry and Molecular Biology, pp. 1248–1262, 2020.","ama":"Smith S, Zhu S, Joos L, et al. The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. <i>Molecular &#38; Cellular Proteomics</i>. 2020;19(8):1248-1262. doi:<a href=\"https://doi.org/10.1074/mcp.ra119.001826\">10.1074/mcp.ra119.001826</a>","chicago":"Smith, S, S Zhu, L Joos, I Roberts, N Nikonorova, LD Vu, E Stes, et al. “The CEP5 Peptide Promotes Abiotic Stress Tolerance, as Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis.” <i>Molecular &#38; Cellular Proteomics</i>. American Society for Biochemistry and Molecular Biology, 2020. <a href=\"https://doi.org/10.1074/mcp.ra119.001826\">https://doi.org/10.1074/mcp.ra119.001826</a>."},"month":"08","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","type":"journal_article","doi":"10.1074/mcp.ra119.001826","date_created":"2020-06-08T10:10:53Z","publication_identifier":{"eissn":["1535-9484"]},"_id":"7949","acknowledgement":"We thank Maria Njo, Sarah De Cokere, Marieke Mispelaere and Darren Wells, for practical assistance, Daniël Van Damme for assistance with image analysis, Marnik Vuylsteke for advice on statistics, Catherine Perrot-Rechenmann for useful discussions, Steffen Lau for critical reading oft he manuscript, and Philip Benfey, Gerd Jürgens, Philippe Nacry, Frederik Börnke, and Frans Tax for sharing materials.","article_processing_charge":"No","page":"1248-1262","external_id":{"pmid":["32404488"],"isi":["000561114000001"]},"title":"The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis","file_date_updated":"2021-05-05T10:10:14Z","abstract":[{"lang":"eng","text":"Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-terminally encoded peptide 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance."}],"publication":"Molecular & Cellular Proteomics","volume":19,"file":[{"file_size":1632311,"date_updated":"2021-05-05T10:10:14Z","content_type":"application/pdf","creator":"kschuh","access_level":"open_access","date_created":"2021-05-05T10:10:14Z","relation":"main_file","checksum":"3f3f37b4a1ba2cfd270fc7733dd89680","success":1,"file_id":"9373","file_name":"2020_MCP_Smith.pdf"}],"day":"01","year":"2020","article_type":"original","publisher":"American Society for Biochemistry and Molecular Biology","date_updated":"2023-09-05T12:17:46Z","status":"public","pmid":1,"publication_status":"published","has_accepted_license":"1","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"        19","author":[{"last_name":"Smith","first_name":"S","full_name":"Smith, S"},{"first_name":"S","last_name":"Zhu","full_name":"Zhu, S"},{"full_name":"Joos, L","last_name":"Joos","first_name":"L"},{"full_name":"Roberts, I","last_name":"Roberts","first_name":"I"},{"first_name":"N","last_name":"Nikonorova","full_name":"Nikonorova, N"},{"last_name":"Vu","first_name":"LD","full_name":"Vu, LD"},{"last_name":"Stes","first_name":"E","full_name":"Stes, E"},{"first_name":"H","last_name":"Cho","full_name":"Cho, H"},{"last_name":"Larrieu","first_name":"A","full_name":"Larrieu, A"},{"last_name":"Xuan","first_name":"W","full_name":"Xuan, W"},{"last_name":"Goodall","first_name":"B","full_name":"Goodall, B"},{"first_name":"B","last_name":"van de Cotte","full_name":"van de Cotte, B"},{"first_name":"JM","last_name":"Waite","full_name":"Waite, JM"},{"first_name":"A","last_name":"Rigal","full_name":"Rigal, A"},{"full_name":"R Harborough, SR","last_name":"R Harborough","first_name":"SR"},{"full_name":"Persiau, G","first_name":"G","last_name":"Persiau"},{"first_name":"S","last_name":"Vanneste","full_name":"Vanneste, S"},{"full_name":"Kirschner, GK","last_name":"Kirschner","first_name":"GK"},{"first_name":"E","last_name":"Vandermarliere","full_name":"Vandermarliere, E"},{"full_name":"Martens, L","first_name":"L","last_name":"Martens"},{"full_name":"Stahl, Y","last_name":"Stahl","first_name":"Y"},{"last_name":"Audenaert","first_name":"D","full_name":"Audenaert, D"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"},{"full_name":"Felix, G","first_name":"G","last_name":"Felix"},{"full_name":"Simon, R","last_name":"Simon","first_name":"R"},{"full_name":"Bennett, M","last_name":"Bennett","first_name":"M"},{"full_name":"Bishopp, A","last_name":"Bishopp","first_name":"A"},{"full_name":"De Jaeger, G","first_name":"G","last_name":"De Jaeger"},{"full_name":"Ljung, K","first_name":"K","last_name":"Ljung"},{"full_name":"Kepinski, S","first_name":"S","last_name":"Kepinski"},{"full_name":"Robert, S","last_name":"Robert","first_name":"S"},{"full_name":"Nemhauser, J","last_name":"Nemhauser","first_name":"J"},{"full_name":"Hwang, I","last_name":"Hwang","first_name":"I"},{"full_name":"Gevaert, K","first_name":"K","last_name":"Gevaert"},{"full_name":"Beeckman, T","last_name":"Beeckman","first_name":"T"},{"full_name":"De Smet, I","first_name":"I","last_name":"De Smet"}],"ddc":["580"],"date_published":"2020-08-01T00:00:00Z"},{"status":"public","publication_status":"published","pmid":1,"publisher":"Springer Nature","article_type":"original","date_updated":"2026-04-07T14:18:57Z","day":"14","year":"2020","file":[{"file_id":"8148","file_name":"2020_NatureComm_Zhang.pdf","success":1,"access_level":"open_access","date_created":"2020-07-22T08:32:55Z","relation":"main_file","file_size":1759490,"content_type":"application/pdf","date_updated":"2020-07-22T08:32:55Z","creator":"dernst"}],"date_published":"2020-07-14T00:00:00Z","ddc":["580"],"corr_author":"1","author":[{"last_name":"Zhang","first_name":"J","full_name":"Zhang, J"},{"full_name":"Mazur, E","last_name":"Mazur","first_name":"E"},{"full_name":"Balla, J","first_name":"J","last_name":"Balla"},{"orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","last_name":"Gallei","first_name":"Michelle C"},{"full_name":"Kalousek, P","first_name":"P","last_name":"Kalousek"},{"full_name":"Medveďová, Z","first_name":"Z","last_name":"Medveďová"},{"full_name":"Li, Y","last_name":"Li","first_name":"Y"},{"last_name":"Wang","first_name":"Y","full_name":"Wang, Y"},{"last_name":"Prat","first_name":"Tomas","id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87","full_name":"Prat, Tomas"},{"first_name":"Mina K","last_name":"Vasileva","full_name":"Vasileva, Mina K","id":"3407EB18-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Reinöhl, V","first_name":"V","last_name":"Reinöhl"},{"last_name":"Procházka","first_name":"S","full_name":"Procházka, S"},{"last_name":"Halouzka","first_name":"R","full_name":"Halouzka, R"},{"last_name":"Tarkowski","first_name":"P","full_name":"Tarkowski, P"},{"last_name":"Luschnig","first_name":"C","full_name":"Luschnig, C"},{"last_name":"Brewer","first_name":"PB","full_name":"Brewer, PB"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"}],"isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"        11","has_accepted_license":"1","date_created":"2020-07-21T08:58:07Z","doi":"10.1038/s41467-020-17252-y","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"11626"}]},"_id":"8138","publication_identifier":{"issn":["2041-1723"]},"acknowledgement":"We are grateful to David Nelson for providing published materials and extremely helpful comments, and Elizabeth Dun and Christine Beveridge for helpful discussions. The research leading to these results has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (742985). This work was also supported by the Beijing Municipal Natural Science Foundation (5192011), Beijing Outstanding University Discipline Program, the National Natural Science Foundation of China (31370309), CEITEC 2020 (LQ1601) project with financial contribution made by the Ministry of Education, Youth and Sports of the Czech Republic within special support paid from the National Program of Sustainability II funds, Australian Research Council (FT180100081), and China Postdoctoral Science Foundation (2019M660864).","scopus_import":"1","citation":{"short":"J. Zhang, E. Mazur, J. Balla, M.C. Gallei, P. Kalousek, Z. Medveďová, Y. Li, Y. Wang, T. Prat, M.K. Vasileva, V. Reinöhl, S. Procházka, R. Halouzka, P. Tarkowski, C. Luschnig, P. Brewer, J. Friml, Nature Communications 11 (2020) 3508.","ieee":"J. Zhang <i>et al.</i>, “Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization,” <i>Nature Communications</i>, vol. 11, no. 1. Springer Nature, p. 3508, 2020.","mla":"Zhang, J., et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin Transport Canalization.” <i>Nature Communications</i>, vol. 11, no. 1, Springer Nature, 2020, p. 3508, doi:<a href=\"https://doi.org/10.1038/s41467-020-17252-y\">10.1038/s41467-020-17252-y</a>.","ama":"Zhang J, Mazur E, Balla J, et al. Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization. <i>Nature Communications</i>. 2020;11(1):3508. doi:<a href=\"https://doi.org/10.1038/s41467-020-17252-y\">10.1038/s41467-020-17252-y</a>","chicago":"Zhang, J, E Mazur, J Balla, Michelle C Gallei, P Kalousek, Z Medveďová, Y Li, et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin Transport Canalization.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-17252-y\">https://doi.org/10.1038/s41467-020-17252-y</a>.","ista":"Zhang J, Mazur E, Balla J, Gallei MC, Kalousek P, Medveďová Z, Li Y, Wang Y, Prat T, Vasileva MK, Reinöhl V, Procházka S, Halouzka R, Tarkowski P, Luschnig C, Brewer P, Friml J. 2020. Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization. Nature Communications. 11(1), 3508.","apa":"Zhang, J., Mazur, E., Balla, J., Gallei, M. C., Kalousek, P., Medveďová, Z., … Friml, J. (2020). Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-17252-y\">https://doi.org/10.1038/s41467-020-17252-y</a>"},"month":"07","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","type":"journal_article","issue":"1","language":[{"iso":"eng"}],"quality_controlled":"1","department":[{"_id":"JiFr"}],"oa_version":"Published Version","oa":1,"file_date_updated":"2020-07-22T08:32:55Z","publication":"Nature Communications","abstract":[{"lang":"eng","text":"Directional transport of the phytohormone auxin is a versatile, plant-specific mechanism regulating many aspects of plant development. The recently identified plant hormones, strigolactones (SLs), are implicated in many plant traits; among others, they modify the phenotypic output of PIN-FORMED (PIN) auxin transporters for fine-tuning of growth and developmental responses. Here, we show in pea and Arabidopsis that SLs target processes dependent on the canalization of auxin flow, which involves auxin feedback on PIN subcellular distribution. D14 receptor- and MAX2 F-box-mediated SL signaling inhibits the formation of auxin-conducting channels after wounding or from artificial auxin sources, during vasculature de novo formation and regeneration. At the cellular level, SLs interfere with auxin effects on PIN polar targeting, constitutive PIN trafficking as well as clathrin-mediated endocytosis. Our results identify a non-transcriptional mechanism of SL action, uncoupling auxin feedback on PIN polarity and trafficking, thereby regulating vascular tissue formation and regeneration."}],"volume":11,"ec_funded":1,"title":"Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"page":"3508","external_id":{"isi":["000550062200004"],"pmid":["32665554"]},"article_processing_charge":"No"},{"status":"public","pmid":1,"publication_status":"published","date_updated":"2026-06-18T19:32:01Z","publisher":"Elsevier","article_type":"original","year":"2020","day":"07","OA_type":"free access","ddc":["580"],"date_published":"2020-09-07T00:00:00Z","author":[{"last_name":"He","first_name":"Peng","full_name":"He, Peng"},{"orcid":"0000-0003-2627-6956","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","full_name":"Zhang, Yuzhou","last_name":"Zhang","first_name":"Yuzhou"},{"full_name":"Xiao, Guanghui","first_name":"Guanghui","last_name":"Xiao"}],"intvolume":"        13","isi":1,"acknowledgement":"We thank Dr. Gai Huang for his comments and help. We apologize to authors whose work could not be cited due to space limitation. No conflict of interest declared.","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.molp.2020.07.006"}],"date_created":"2020-08-16T22:00:57Z","doi":"10.1016/j.molp.2020.07.006","publication_identifier":{"issn":["1674-2052"],"eissn":["1752-9867"]},"_id":"8271","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"09","type":"journal_article","scopus_import":"1","citation":{"ama":"He P, Zhang Y, Xiao G. Origin of a subgenome and genome evolution of allotetraploid cotton species. <i>Molecular Plant</i>. 2020;13(9):1238-1240. doi:<a href=\"https://doi.org/10.1016/j.molp.2020.07.006\">10.1016/j.molp.2020.07.006</a>","chicago":"He, Peng, Yuzhou Zhang, and Guanghui Xiao. “Origin of a Subgenome and Genome Evolution of Allotetraploid Cotton Species.” <i>Molecular Plant</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.molp.2020.07.006\">https://doi.org/10.1016/j.molp.2020.07.006</a>.","short":"P. He, Y. Zhang, G. Xiao, Molecular Plant 13 (2020) 1238–1240.","mla":"He, Peng, et al. “Origin of a Subgenome and Genome Evolution of Allotetraploid Cotton Species.” <i>Molecular Plant</i>, vol. 13, no. 9, Elsevier, 2020, pp. 1238–40, doi:<a href=\"https://doi.org/10.1016/j.molp.2020.07.006\">10.1016/j.molp.2020.07.006</a>.","ieee":"P. He, Y. Zhang, and G. Xiao, “Origin of a subgenome and genome evolution of allotetraploid cotton species,” <i>Molecular Plant</i>, vol. 13, no. 9. Elsevier, pp. 1238–1240, 2020.","apa":"He, P., Zhang, Y., &#38; Xiao, G. (2020). Origin of a subgenome and genome evolution of allotetraploid cotton species. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2020.07.006\">https://doi.org/10.1016/j.molp.2020.07.006</a>","ista":"He P, Zhang Y, Xiao G. 2020. Origin of a subgenome and genome evolution of allotetraploid cotton species. Molecular Plant. 13(9), 1238–1240."},"OA_place":"publisher","issue":"9","language":[{"iso":"eng"}],"quality_controlled":"1","oa_version":"Published Version","oa":1,"department":[{"_id":"JiFr"}],"publication":"Molecular Plant","volume":13,"title":"Origin of a subgenome and genome evolution of allotetraploid cotton species","external_id":{"isi":["000566895400007"],"pmid":["32688032"]},"page":"1238-1240","article_processing_charge":"No"},{"has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"intvolume":"        11","author":[{"first_name":"Ioanna","last_name":"Antoniadi","full_name":"Antoniadi, Ioanna"},{"full_name":"Novák, Ondřej","first_name":"Ondřej","last_name":"Novák"},{"last_name":"Gelová","first_name":"Zuzana","orcid":"0000-0003-4783-1752","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","full_name":"Gelová, Zuzana"},{"last_name":"Johnson","first_name":"Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","full_name":"Johnson, Alexander J"},{"last_name":"Plíhal","first_name":"Ondřej","full_name":"Plíhal, Ondřej"},{"last_name":"Simerský","first_name":"Radim","full_name":"Simerský, Radim"},{"full_name":"Mik, Václav","last_name":"Mik","first_name":"Václav"},{"last_name":"Vain","first_name":"Thomas","full_name":"Vain, Thomas"},{"full_name":"Mateo-Bonmatí, Eduardo","last_name":"Mateo-Bonmatí","first_name":"Eduardo"},{"full_name":"Karady, Michal","first_name":"Michal","last_name":"Karady"},{"last_name":"Pernisová","first_name":"Markéta","full_name":"Pernisová, Markéta"},{"full_name":"Plačková, Lenka","last_name":"Plačková","first_name":"Lenka"},{"last_name":"Opassathian","first_name":"Korawit","full_name":"Opassathian, Korawit"},{"full_name":"Hejátko, Jan","last_name":"Hejátko","first_name":"Jan"},{"last_name":"Robert","first_name":"Stéphanie","full_name":"Robert, Stéphanie"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"},{"full_name":"Doležal, Karel","last_name":"Doležal","first_name":"Karel"},{"last_name":"Ljung","first_name":"Karin","full_name":"Ljung, Karin"},{"full_name":"Turnbull, Colin","first_name":"Colin","last_name":"Turnbull"}],"date_published":"2020-08-27T00:00:00Z","ddc":["580"],"file":[{"success":1,"file_name":"2020_NatureComm_Antoniadi.pdf","file_id":"8936","creator":"dernst","file_size":3526415,"date_updated":"2020-12-10T12:23:56Z","content_type":"application/pdf","relation":"main_file","date_created":"2020-12-10T12:23:56Z","checksum":"5b96f39b598de7510cfefefb819b9a6d","access_level":"open_access"}],"day":"27","year":"2020","article_type":"original","publisher":"Springer Nature","date_updated":"2026-04-03T09:25:48Z","status":"public","publication_status":"published","pmid":1,"project":[{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"},{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"}],"article_processing_charge":"No","external_id":{"isi":["000567931000001"],"pmid":["32855409"]},"acknowledged_ssus":[{"_id":"Bio"}],"title":"Cell-surface receptors enable perception of extracellular cytokinins","article_number":"4284","file_date_updated":"2020-12-10T12:23:56Z","publication":"Nature Communications","abstract":[{"text":"Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.","lang":"eng"}],"volume":11,"ec_funded":1,"department":[{"_id":"JiFr"}],"oa_version":"Published Version","oa":1,"quality_controlled":"1","language":[{"iso":"eng"}],"scopus_import":"1","citation":{"apa":"Antoniadi, I., Novák, O., Gelová, Z., Johnson, A. J., Plíhal, O., Simerský, R., … Turnbull, C. (2020). Cell-surface receptors enable perception of extracellular cytokinins. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-17700-9\">https://doi.org/10.1038/s41467-020-17700-9</a>","ista":"Antoniadi I, Novák O, Gelová Z, Johnson AJ, Plíhal O, Simerský R, Mik V, Vain T, Mateo-Bonmatí E, Karady M, Pernisová M, Plačková L, Opassathian K, Hejátko J, Robert S, Friml J, Doležal K, Ljung K, Turnbull C. 2020. Cell-surface receptors enable perception of extracellular cytokinins. Nature Communications. 11, 4284.","chicago":"Antoniadi, Ioanna, Ondřej Novák, Zuzana Gelová, Alexander J Johnson, Ondřej Plíhal, Radim Simerský, Václav Mik, et al. “Cell-Surface Receptors Enable Perception of Extracellular Cytokinins.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-17700-9\">https://doi.org/10.1038/s41467-020-17700-9</a>.","ama":"Antoniadi I, Novák O, Gelová Z, et al. Cell-surface receptors enable perception of extracellular cytokinins. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-17700-9\">10.1038/s41467-020-17700-9</a>","mla":"Antoniadi, Ioanna, et al. “Cell-Surface Receptors Enable Perception of Extracellular Cytokinins.” <i>Nature Communications</i>, vol. 11, 4284, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-17700-9\">10.1038/s41467-020-17700-9</a>.","ieee":"I. Antoniadi <i>et al.</i>, “Cell-surface receptors enable perception of extracellular cytokinins,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","short":"I. Antoniadi, O. Novák, Z. Gelová, A.J. Johnson, O. Plíhal, R. Simerský, V. Mik, T. Vain, E. Mateo-Bonmatí, M. Karady, M. Pernisová, L. Plačková, K. Opassathian, J. Hejátko, S. Robert, J. Friml, K. Doležal, K. Ljung, C. Turnbull, Nature Communications 11 (2020)."},"month":"08","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","type":"journal_article","date_created":"2020-09-06T22:01:13Z","doi":"10.1038/s41467-020-17700-9","_id":"8337","publication_identifier":{"eissn":["2041-1723"]},"acknowledgement":"We thank Bruno Müller and Aaron Rashotte for critical discussions and provision of plant lines used in this work, Roger Granbom and Tamara Hernández Verdeja (UPSC, Umeå, Sweden) for technical assistance and providing materials, Zuzana Pěkná and Karolina Wojewodová (CRH, Palacký University, Olomouc, Czech Republic) for help with cytokinin receptor binding assays, and David Zalabák (CRH, Palacký University, Olomouc, Czech Republic) for provision of vector pINIIIΔEH expressing CRE1/AHK4. The bioimaging facility of IST Austria, the Swedish Metabolomics Centre and the IST Austria Bio-Imaging facility are acknowledged for support. The work was funded by the European Molecular Biology Organization (EMBO ASTF 297-2013) (I.A.), Development—The Company of Biologists (DEVTF2012) (I.A.; C.T.), Plant Fellows (the International Post doc Fellowship Programme in Plant Sciences, 267423) (I.A.; K.L.), the Swedish Research Council (621-2014-4514) (K.L.), UPSC Berzelii Center for Forest Biotechnology (Vinnova 2012-01560), Kempestiftelserna (JCK-2711) (K.L.) and (JCK-1811) (E.-M.B., K.L.). The Ministry of Education, Youth and Sports of the Czech Republic via the European Regional Development Fund-Project “Plants as a tool for sustainable global development” (CZ.02.1.01/0.0/0.0/16_019/0000827) (O.N., O.P., R.S., V.M., L.P., K.D.) and project CEITEC 2020 (LQ1601) (M.P., J.H.) provided support, as did the Czech Science Foundation via projects GP14-30004P (M.P.) and 16-04184S (O.P., K.D., O.N.), Vetenskapsrådet and Vinnova (Verket för Innovationssystem) (T.V., S.R.), Knut och Alice Wallenbergs Stiftelse via “Shapesystem” grant number 2012.0050. A.J. was supported by the Austria Science Fund (FWF): I03630 to J.F. The research leading to these results received funding from European Union’s Horizon 2020 programme (ERC grant no. 742985) and FWO-FWF joint project G0E5718N to J.F."},{"title":"Novel insights into PIN polarity regulation during Arabidopsis development","alternative_title":["ISTA Thesis"],"abstract":[{"lang":"eng","text":"The plant hormone auxin plays indispensable roles in plant growth and development. An essential level of regulation in auxin action is the directional auxin transport within cells. The establishment of auxin gradient in plant tissue has been attributed to local auxin biosynthesis and directional intercellular auxin transport, which both are controlled by various environmental and developmental signals. It is well established that asymmetric auxin distribution in cells is achieved by polarly localized PIN-FORMED (PIN) auxin efflux transporters. Despite the initial insights into cellular mechanisms of PIN polarization obtained from the last decades, the molecular mechanism and specific regulators mediating PIN polarization remains elusive. In this thesis, we aim to find novel players in PIN subcellular polarity regulation during Arabidopsis development. We first characterize the physiological effect of piperonylic acid (PA) on Arabidopsis hypocotyl gravitropic bending and PIN polarization. Secondly, we reveal the importance of SCFTIR1/AFB auxin signaling pathway in shoot gravitropism bending termination. In addition, we also explore the role of myosin XI complex, and actin cytoskeleton in auxin feedback regulation on PIN polarity. In Chapter 1, we give an overview of the current knowledge about PIN-mediated auxin fluxes in various plant tropic responses. In Chapter 2, we study the physiological effect of PA on shoot gravitropic bending. Our results show that PA treatment inhibits auxin-mediated PIN3 repolarization by interfering with PINOID and PIN3 phosphorylation status, ultimately leading to hyperbending hypocotyls. In Chapter 3, we provide evidence to show that the SCFTIR1/AFB nuclear auxin signaling pathway is crucial and required for auxin-mediated PIN3 repolarization and shoot gravitropic bending termination. In Chapter 4, we perform a phosphoproteomics approach and identify the motor protein Myosin XI and its binding protein, the MadB2 family, as an essential regulator of PIN polarity for auxin-canalization related developmental processes. In Chapter 5, we demonstrate the vital role of actin cytoskeleton in auxin feedback on PIN polarity by regulating PIN subcellular trafficking. Overall, the data presented in this PhD thesis brings novel insights into the PIN polar localization regulation that resulted in the (re)establishment of the polar auxin flow and gradient in response to environmental stimuli during plant development."}],"file_date_updated":"2021-10-01T13:33:02Z","supervisor":[{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"No","page":"164","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"type":"dissertation","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","month":"09","citation":{"short":"H. Han, Novel Insights into PIN Polarity Regulation during Arabidopsis Development, Institute of Science and Technology Austria, 2020.","mla":"Han, Huibin. <i>Novel Insights into PIN Polarity Regulation during Arabidopsis Development</i>. Institute of Science and Technology Austria, 2020, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8589\">10.15479/AT:ISTA:8589</a>.","ieee":"H. Han, “Novel insights into PIN polarity regulation during Arabidopsis development,” Institute of Science and Technology Austria, 2020.","ama":"Han H. Novel insights into PIN polarity regulation during Arabidopsis development. 2020. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8589\">10.15479/AT:ISTA:8589</a>","chicago":"Han, Huibin. “Novel Insights into PIN Polarity Regulation during Arabidopsis Development.” Institute of Science and Technology Austria, 2020. <a href=\"https://doi.org/10.15479/AT:ISTA:8589\">https://doi.org/10.15479/AT:ISTA:8589</a>.","ista":"Han H. 2020. Novel insights into PIN polarity regulation during Arabidopsis development. Institute of Science and Technology Austria.","apa":"Han, H. (2020). <i>Novel insights into PIN polarity regulation during Arabidopsis development</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:8589\">https://doi.org/10.15479/AT:ISTA:8589</a>"},"degree_awarded":"PhD","acknowledgement":"I also want to thank the China Scholarship Council for supporting my study during the year from 2015 to 2019. I also want to thank IST facilities – the Bioimaging facility, the media kitchen, the plant facility and all of the campus services, for their support.","publication_identifier":{"issn":["2663-337X"]},"_id":"8589","related_material":{"record":[{"id":"7643","relation":"part_of_dissertation","status":"public"}]},"doi":"10.15479/AT:ISTA:8589","date_created":"2020-09-30T14:50:51Z","oa":1,"oa_version":"Published Version","department":[{"_id":"JiFr"}],"OA_place":"publisher","language":[{"iso":"eng"}],"author":[{"first_name":"Huibin","last_name":"Han","full_name":"Han, Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87"}],"corr_author":"1","date_published":"2020-09-30T00:00:00Z","ddc":["580"],"has_accepted_license":"1","date_updated":"2026-06-18T19:25:52Z","publisher":"Institute of Science and Technology Austria","publication_status":"published","status":"public","file":[{"file_size":49198118,"date_updated":"2020-09-30T14:50:20Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","creator":"dernst","access_level":"closed","relation":"source_file","date_created":"2020-09-30T14:50:20Z","checksum":"c4bda1947d4c09c428ac9ce667b02327","file_id":"8590","file_name":"2020_Han_Thesis.docx"},{"file_name":"2020_Han_Thesis.pdf","file_id":"8591","checksum":"3f4f5d1718c2230adf30639ecaf8a00b","date_created":"2020-09-30T14:49:59Z","relation":"main_file","access_level":"open_access","creator":"dernst","date_updated":"2021-10-01T13:33:02Z","content_type":"application/pdf","file_size":15513963}],"year":"2020","day":"30"},{"date_updated":"2026-06-18T19:35:07Z","publisher":"American Society of Plant Biologists","article_type":"original","pmid":1,"publication_status":"published","status":"public","year":"2020","day":"01","author":[{"first_name":"D","last_name":"Liu","full_name":"Liu, D"},{"last_name":"Kumar","first_name":"R","full_name":"Kumar, R"},{"first_name":"Claus","last_name":"LAN","full_name":"LAN, Claus"},{"first_name":"Alexander J","last_name":"Johnson","full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2739-8843"},{"full_name":"Siao, W","first_name":"W","last_name":"Siao"},{"full_name":"Vanhoutte, I","last_name":"Vanhoutte","first_name":"I"},{"first_name":"P","last_name":"Wang","full_name":"Wang, P"},{"first_name":"KW","last_name":"Bender","full_name":"Bender, KW"},{"last_name":"Yperman","first_name":"K","full_name":"Yperman, K"},{"first_name":"S","last_name":"Martins","full_name":"Martins, S"},{"last_name":"Zhao","first_name":"X","full_name":"Zhao, X"},{"last_name":"Vert","first_name":"G","full_name":"Vert, G"},{"last_name":"Van Damme","first_name":"D","full_name":"Van Damme, D"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"},{"last_name":"Russinova","first_name":"E","full_name":"Russinova, E"}],"ddc":["580"],"date_published":"2020-11-01T00:00:00Z","intvolume":"        32","isi":1,"type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"11","citation":{"ieee":"D. Liu <i>et al.</i>, “Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif,” <i>Plant Cell</i>, vol. 32, no. 11. American Society of Plant Biologists, pp. 3598–3612, 2020.","mla":"Liu, D., et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>, vol. 32, no. 11, American Society of Plant Biologists, 2020, pp. 3598–612, doi:<a href=\"https://doi.org/10.1105/tpc.20.00384\">10.1105/tpc.20.00384</a>.","short":"D. Liu, R. Kumar, C. LAN, A.J. Johnson, W. Siao, I. Vanhoutte, P. Wang, K. Bender, K. Yperman, S. Martins, X. Zhao, G. Vert, D. Van Damme, J. Friml, E. Russinova, Plant Cell 32 (2020) 3598–3612.","chicago":"Liu, D, R Kumar, Claus LAN, Alexander J Johnson, W Siao, I Vanhoutte, P Wang, et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1105/tpc.20.00384\">https://doi.org/10.1105/tpc.20.00384</a>.","ama":"Liu D, Kumar R, LAN C, et al. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. <i>Plant Cell</i>. 2020;32(11):3598-3612. doi:<a href=\"https://doi.org/10.1105/tpc.20.00384\">10.1105/tpc.20.00384</a>","ista":"Liu D, Kumar R, LAN C, Johnson AJ, Siao W, Vanhoutte I, Wang P, Bender K, Yperman K, Martins S, Zhao X, Vert G, Van Damme D, Friml J, Russinova E. 2020. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. Plant Cell. 32(11), 3598–3612.","apa":"Liu, D., Kumar, R., LAN, C., Johnson, A. J., Siao, W., Vanhoutte, I., … Russinova, E. (2020). Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.20.00384\">https://doi.org/10.1105/tpc.20.00384</a>"},"scopus_import":"1","main_file_link":[{"url":"https://europepmc.org/article/MED/32958564","open_access":"1"}],"_id":"8607","publication_identifier":{"eissn":["1532-298x"],"issn":["1040-4651"]},"date_created":"2020-10-05T12:45:16Z","doi":"10.1105/tpc.20.00384","oa":1,"oa_version":"Published Version","department":[{"_id":"JiFr"}],"quality_controlled":"1","language":[{"iso":"eng"}],"issue":"11","title":"Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif","ec_funded":1,"volume":32,"publication":"Plant Cell","abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles through the recognition of motifs based on tyrosine or di-leucine in their cytoplasmic tails. However, in plants, very little is known on how PM proteins are sorted for CME and whether similar motifs are required. In Arabidopsis thaliana, the brassinosteroid (BR) receptor, BR INSENSITIVE1 (BRI1), undergoes endocytosis that depends on clathrin and AP-2. Here we demonstrate that BRI1 binds directly to the medium AP-2 subunit, AP2M. The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed tyrosine-based endocytic motifs. The tyrosine-to-phenylalanine substitution in Y898KAI reduced BRI1 internalization without affecting its kinase activity. Consistently, plants carrying the BRI1Y898F mutation were hypersensitive to BRs. Our study demonstrates that AP-2-dependent internalization of PM proteins via the recognition of functional tyrosine motifs also operates in plants."}],"article_processing_charge":"No","page":"3598-3612","external_id":{"isi":["000600226800021"],"pmid":["32958564"]},"project":[{"grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF"},{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"}]},{"intvolume":"       370","isi":1,"author":[{"first_name":"Jakub","last_name":"Hajny","orcid":"0000-0003-2140-7195","full_name":"Hajny, Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Prat","first_name":"Tomas","id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87","full_name":"Prat, Tomas"},{"first_name":"N","last_name":"Rydza","full_name":"Rydza, N"},{"first_name":"Lesia","last_name":"Rodriguez Solovey","orcid":"0000-0002-7244-7237","full_name":"Rodriguez Solovey, Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","last_name":"Tan","first_name":"Shutang"},{"orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","first_name":"Inge","last_name":"Verstraeten"},{"first_name":"David","last_name":"Domjan","orcid":"0000-0003-2267-106X","full_name":"Domjan, David","id":"C684CD7A-257E-11EA-9B6F-D8588B4F947F"},{"full_name":"Mazur, E","last_name":"Mazur","first_name":"E"},{"first_name":"E","last_name":"Smakowska-Luzan","full_name":"Smakowska-Luzan, E"},{"full_name":"Smet, W","last_name":"Smet","first_name":"W"},{"full_name":"Mor, E","last_name":"Mor","first_name":"E"},{"full_name":"Nolf, J","first_name":"J","last_name":"Nolf"},{"first_name":"B","last_name":"Yang","full_name":"Yang, B"},{"first_name":"W","last_name":"Grunewald","full_name":"Grunewald, W"},{"first_name":"Gergely","last_name":"Molnar","full_name":"Molnar, Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Y","last_name":"Belkhadir","full_name":"Belkhadir, Y"},{"full_name":"De Rybel, B","first_name":"B","last_name":"De Rybel"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"ddc":["580"],"date_published":"2020-10-30T00:00:00Z","year":"2020","day":"30","date_updated":"2026-06-18T19:35:33Z","article_type":"original","publisher":"American Association for the Advancement of Science","status":"public","publication_status":"published","pmid":1,"article_processing_charge":"No","page":"550-557","external_id":{"pmid":["33122378"],"isi":["000583031800041"]},"project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"},{"_id":"2699E3D2-B435-11E9-9278-68D0E5697425","grant_number":"25239","name":"Cell surface receptor complexes for PIN polarity and auxin-mediated development"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"title":"Receptor kinase module targets PIN-dependent auxin transport during canalization","publication":"Science","abstract":[{"lang":"eng","text":"Spontaneously arising channels that transport the phytohormone auxin provide positional cues for self-organizing aspects of plant development such as flexible vasculature regeneration or its patterning during leaf venation. The auxin canalization hypothesis proposes a feedback between auxin signaling and transport as the underlying mechanism, but molecular players await discovery. We identified part of the machinery that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like kinase) interact with and phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization, which macroscopically manifests as defects in leaf venation and vasculature regeneration after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback that coordinates polarization of individual cells during auxin canalization."}],"volume":370,"ec_funded":1,"oa_version":"Published Version","oa":1,"department":[{"_id":"JiFr"}],"issue":"6516","language":[{"iso":"eng"}],"quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"10","type":"journal_article","scopus_import":"1","citation":{"apa":"Hajny, J., Prat, T., Rydza, N., Rodriguez Solovey, L., Tan, S., Verstraeten, I., … Friml, J. (2020). Receptor kinase module targets PIN-dependent auxin transport during canalization. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aba3178\">https://doi.org/10.1126/science.aba3178</a>","ista":"Hajny J, Prat T, Rydza N, Rodriguez Solovey L, Tan S, Verstraeten I, Domjan D, Mazur E, Smakowska-Luzan E, Smet W, Mor E, Nolf J, Yang B, Grunewald W, Molnar G, Belkhadir Y, De Rybel B, Friml J. 2020. Receptor kinase module targets PIN-dependent auxin transport during canalization. Science. 370(6516), 550–557.","chicago":"Hajny, Jakub, Tomas Prat, N Rydza, Lesia Rodriguez Solovey, Shutang Tan, Inge Verstraeten, David Domjan, et al. “Receptor Kinase Module Targets PIN-Dependent Auxin Transport during Canalization.” <i>Science</i>. American Association for the Advancement of Science, 2020. <a href=\"https://doi.org/10.1126/science.aba3178\">https://doi.org/10.1126/science.aba3178</a>.","ama":"Hajny J, Prat T, Rydza N, et al. Receptor kinase module targets PIN-dependent auxin transport during canalization. <i>Science</i>. 2020;370(6516):550-557. doi:<a href=\"https://doi.org/10.1126/science.aba3178\">10.1126/science.aba3178</a>","mla":"Hajny, Jakub, et al. “Receptor Kinase Module Targets PIN-Dependent Auxin Transport during Canalization.” <i>Science</i>, vol. 370, no. 6516, American Association for the Advancement of Science, 2020, pp. 550–57, doi:<a href=\"https://doi.org/10.1126/science.aba3178\">10.1126/science.aba3178</a>.","ieee":"J. Hajny <i>et al.</i>, “Receptor kinase module targets PIN-dependent auxin transport during canalization,” <i>Science</i>, vol. 370, no. 6516. American Association for the Advancement of Science, pp. 550–557, 2020.","short":"J. Hajny, T. Prat, N. Rydza, L. Rodriguez Solovey, S. Tan, I. Verstraeten, D. Domjan, E. Mazur, E. Smakowska-Luzan, W. Smet, E. Mor, J. Nolf, B. Yang, W. Grunewald, G. Molnar, Y. Belkhadir, B. De Rybel, J. Friml, Science 370 (2020) 550–557."},"main_file_link":[{"url":"https://europepmc.org/article/MED/33122378#free-full-text","open_access":"1"}],"acknowledgement":"We acknowledge M. Glanc and Y. Zhang for providing entryclones; Vienna Biocenter Core Facilities (VBCF) for recombinantprotein production and purification; Vienna Biocenter Massspectrometry Facility, Bioimaging, and Life Science Facilities at IST Austria and Proteomics Core Facility CEITEC for a great assistance.Funding:This project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement 742985) and Austrian Science Fund (FWF): I 3630-B25 to J.F.and by grants from the Austrian Academy of Science through the Gregor Mendel Institute (Y.B.) and the Austrian Agency for International Cooperation in Education and Research (D.D.); the Netherlands Organization for Scientific Research (NWO; VIDI-864.13.001) (W.S.); the Research Foundation–Flanders (FWO;Odysseus II G0D0515N) and a European Research Council grant (ERC; StG TORPEDO; 714055) to B.D.R., B.Y., and E.M.; and the Hertha Firnberg Programme postdoctoral fellowship (T-947) from the FWF Austrian Science Fund to E.S.-L.; J.H. is the recipient of a DOC Fellowship of the Austrian Academy of Sciences at IST Austria.","doi":"10.1126/science.aba3178","date_created":"2020-11-02T10:04:46Z","related_material":{"link":[{"url":"https://ist.ac.at/en/news/molecular-compass-for-cell-orientation/","relation":"press_release","description":"News on IST Homepage"}]},"_id":"8721","publication_identifier":{"eissn":["1095-9203"],"issn":["0036-8075"]}},{"date_created":"2020-12-13T23:01:21Z","doi":"10.1016/j.celrep.2020.108463","_id":"8943","publication_identifier":{"eissn":["2211-1247"]},"related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/plants-on-aspirin/"}]},"acknowledgement":"We thank Drs. Sebastian Bednarek (University of Wisconsin-Madison), Niko Geldner (University of Lausanne), and Karin Schumacher (Heidelberg University) for kindly sharing published Arabidopsis lines; Dr. Satoshi Naramoto for the pPIN2::PIN2-GFP; pVHA-a1::VHA-a1-mRFP reporter; the staff at the Life Science Facility and Bioimaging Facility, Monika Hrtyan, and Dorota Jaworska at IST Austria for technical support; and Drs. Su Tang (Texas A&M University),\r\nMelinda Abas (BOKU), Eva Benkova´ (IST Austria), Christian Luschnig (BOKU), Bartel Vanholme (Gent University), and the Friml group for valuable discussions. The research leading to these findings was funded by the European Union’s Horizon 2020 program (ERC grant agreement no. 742985, to J.F.), the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no.\r\n291734, the Swiss National Funds (31003A_165877, to M.G.), the Ministry of Education, Youth, and Sports of the Czech Republic (project no. CZ.02.1.01/0.0/0.0/16_019/0000738, EU Operational Programme ‘‘Research, development and education and Centre for Plant Experimental Biology’’), and the EU Operational Programme Prague - Competitiveness (project no. CZ.2.16/3.1.00/21519). S.T. was funded by a European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). X.Z. was partly supported by a PhD scholarship from the China Scholarship Council.","scopus_import":"1","citation":{"ieee":"S. Tan <i>et al.</i>, “Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development,” <i>Cell Reports</i>, vol. 33, no. 9. Elsevier, 2020.","mla":"Tan, Shutang, et al. “Non-Steroidal Anti-Inflammatory Drugs Target TWISTED DWARF1-Regulated Actin Dynamics and Auxin Transport-Mediated Plant Development.” <i>Cell Reports</i>, vol. 33, no. 9, 108463, Elsevier, 2020, doi:<a href=\"https://doi.org/10.1016/j.celrep.2020.108463\">10.1016/j.celrep.2020.108463</a>.","short":"S. Tan, M. Di Donato, M. Glanc, X. Zhang, P. Klíma, J. Liu, A. Bailly, N. Ferro, J. Petrášek, M. Geisler, J. Friml, Cell Reports 33 (2020).","chicago":"Tan, Shutang, Martin Di Donato, Matous Glanc, Xixi Zhang, Petr Klíma, Jie Liu, Aurélien Bailly, et al. “Non-Steroidal Anti-Inflammatory Drugs Target TWISTED DWARF1-Regulated Actin Dynamics and Auxin Transport-Mediated Plant Development.” <i>Cell Reports</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.celrep.2020.108463\">https://doi.org/10.1016/j.celrep.2020.108463</a>.","ama":"Tan S, Di Donato M, Glanc M, et al. Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development. <i>Cell Reports</i>. 2020;33(9). doi:<a href=\"https://doi.org/10.1016/j.celrep.2020.108463\">10.1016/j.celrep.2020.108463</a>","ista":"Tan S, Di Donato M, Glanc M, Zhang X, Klíma P, Liu J, Bailly A, Ferro N, Petrášek J, Geisler M, Friml J. 2020. Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development. Cell Reports. 33(9), 108463.","apa":"Tan, S., Di Donato, M., Glanc, M., Zhang, X., Klíma, P., Liu, J., … Friml, J. (2020). Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2020.108463\">https://doi.org/10.1016/j.celrep.2020.108463</a>"},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","month":"12","type":"journal_article","issue":"9","language":[{"iso":"eng"}],"quality_controlled":"1","department":[{"_id":"JiFr"}],"oa_version":"Published Version","oa":1,"file_date_updated":"2020-12-14T07:33:39Z","publication":"Cell Reports","abstract":[{"text":"The widely used non-steroidal anti-inflammatory drugs (NSAIDs) are derivatives of the phytohormone salicylic acid (SA). SA is well known to regulate plant immunity and development, whereas there have been few reports focusing on the effects of NSAIDs in plants. Our studies here reveal that NSAIDs exhibit largely overlapping physiological activities to SA in the model plant Arabidopsis. NSAID treatments lead to shorter and agravitropic primary roots and inhibited lateral root organogenesis. Notably, in addition to the SA-like action, which in roots involves binding to the protein phosphatase 2A (PP2A), NSAIDs also exhibit PP2A-independent effects. Cell biological and biochemical analyses reveal that many NSAIDs bind directly to and inhibit the chaperone activity of TWISTED DWARF1, thereby regulating actin cytoskeleton dynamics and subsequent endosomal trafficking. Our findings uncover an unexpected bioactivity of human pharmaceuticals in plants and provide insights into the molecular mechanism underlying the cellular action of this class of anti-inflammatory compounds.","lang":"eng"}],"ec_funded":1,"volume":33,"article_number":"108463","title":"Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"},{"name":"Molecular Mechanism underlying Salicylic Acid Regulation of Endocytic Trafficking in Arabidopsis","_id":"256FEF10-B435-11E9-9278-68D0E5697425","grant_number":"723-2015"}],"external_id":{"isi":["000595658100018"],"pmid":["33264621"]},"article_processing_charge":"Yes","status":"public","pmid":1,"publication_status":"published","publisher":"Elsevier","article_type":"original","date_updated":"2026-04-03T09:30:47Z","day":"01","year":"2020","file":[{"date_updated":"2020-12-14T07:33:39Z","content_type":"application/pdf","file_size":8056434,"creator":"dernst","access_level":"open_access","relation":"main_file","date_created":"2020-12-14T07:33:39Z","checksum":"ed18cba0fb48ed2e789381a54cc21904","success":1,"file_id":"8948","file_name":"2020_CellReports_Tan.pdf"}],"date_published":"2020-12-01T00:00:00Z","ddc":["580"],"corr_author":"1","author":[{"full_name":"Tan, Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0471-8285","first_name":"Shutang","last_name":"Tan"},{"full_name":"Di Donato, Martin","last_name":"Di Donato","first_name":"Martin"},{"first_name":"Matous","last_name":"Glanc","full_name":"Glanc, Matous","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","orcid":"0000-0003-0619-7783"},{"last_name":"Zhang","first_name":"Xixi","orcid":"0000-0001-7048-4627","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","full_name":"Zhang, Xixi"},{"first_name":"Petr","last_name":"Klíma","full_name":"Klíma, Petr"},{"last_name":"Liu","first_name":"Jie","full_name":"Liu, Jie"},{"full_name":"Bailly, Aurélien","last_name":"Bailly","first_name":"Aurélien"},{"full_name":"Ferro, Noel","first_name":"Noel","last_name":"Ferro"},{"last_name":"Petrášek","first_name":"Jan","full_name":"Petrášek, Jan"},{"full_name":"Geisler, Markus","first_name":"Markus","last_name":"Geisler"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"}],"isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"        33","has_accepted_license":"1"},{"author":[{"orcid":"0000-0003-2627-6956","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","full_name":"Zhang, Yuzhou","last_name":"Zhang","first_name":"Yuzhou"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"date_published":"2020-02-01T00:00:00Z","ddc":["580"],"corr_author":"1","has_accepted_license":"1","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"       225","publisher":"Wiley","article_type":"original","date_updated":"2025-04-14T07:45:04Z","status":"public","publication_status":"published","pmid":1,"file":[{"file_size":717345,"content_type":"application/pdf","date_updated":"2020-11-18T16:42:48Z","creator":"dernst","access_level":"open_access","relation":"main_file","checksum":"cd42ffdb381fd52812b9583d4d407139","date_created":"2020-11-18T16:42:48Z","success":1,"file_id":"8772","file_name":"2020_NewPhytologist_Zhang.pdf"}],"day":"01","year":"2020","title":"Auxin guides roots to avoid obstacles during gravitropic growth","file_date_updated":"2020-11-18T16:42:48Z","publication":"New Phytologist","volume":225,"ec_funded":1,"project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"},{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"},{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme"}],"article_processing_charge":"Yes (via OA deal)","page":"1049-1052","external_id":{"pmid":["31603260"],"isi":["000489638800001"]},"scopus_import":"1","citation":{"apa":"Zhang, Y., &#38; Friml, J. (2020). Auxin guides roots to avoid obstacles during gravitropic growth. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16203\">https://doi.org/10.1111/nph.16203</a>","ista":"Zhang Y, Friml J. 2020. Auxin guides roots to avoid obstacles during gravitropic growth. New Phytologist. 225(3), 1049–1052.","chicago":"Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during Gravitropic Growth.” <i>New Phytologist</i>. Wiley, 2020. <a href=\"https://doi.org/10.1111/nph.16203\">https://doi.org/10.1111/nph.16203</a>.","ama":"Zhang Y, Friml J. Auxin guides roots to avoid obstacles during gravitropic growth. <i>New Phytologist</i>. 2020;225(3):1049-1052. doi:<a href=\"https://doi.org/10.1111/nph.16203\">10.1111/nph.16203</a>","mla":"Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during Gravitropic Growth.” <i>New Phytologist</i>, vol. 225, no. 3, Wiley, 2020, pp. 1049–52, doi:<a href=\"https://doi.org/10.1111/nph.16203\">10.1111/nph.16203</a>.","ieee":"Y. Zhang and J. Friml, “Auxin guides roots to avoid obstacles during gravitropic growth,” <i>New Phytologist</i>, vol. 225, no. 3. Wiley, pp. 1049–1052, 2020.","short":"Y. Zhang, J. Friml, New Phytologist 225 (2020) 1049–1052."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"02","type":"journal_article","doi":"10.1111/nph.16203","date_created":"2019-11-12T11:41:32Z","publication_identifier":{"issn":["0028-646x"],"eissn":["1469-8137"]},"_id":"6997","department":[{"_id":"JiFr"}],"oa":1,"oa_version":"Published Version","issue":"3","quality_controlled":"1","language":[{"iso":"eng"}]},{"external_id":{"pmid":["31760231"],"isi":["000521120600007"]},"article_processing_charge":"No","page":"43-49","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"publication":"Current Opinion in Plant Biology","abstract":[{"text":"The phytohormone auxin acts as an amazingly versatile coordinator of plant growth and development. With its morphogen-like properties, auxin controls sites and timing of differentiation and/or growth responses both, in quantitative and qualitative terms. Specificity in the auxin response depends largely on distinct modes of signal transmission, by which individual cells perceive and convert auxin signals into a remarkable diversity of responses. The best understood, or so-called canonical mechanism of auxin perception ultimately results in variable adjustments of the cellular transcriptome, via a short, nuclear signal transduction pathway. Additional findings that accumulated over decades implied that an additional, presumably, cell surface-based auxin perception mechanism mediates very rapid cellular responses and decisively contributes to the cell's overall hormonal response. Recent investigations into both, nuclear and cell surface auxin signalling challenged this assumed partition of roles for different auxin signalling pathways and revealed an unexpected complexity in transcriptional and non-transcriptional cellular responses mediated by auxin.","lang":"eng"}],"volume":53,"ec_funded":1,"title":"Auxin signalling in growth: Schrödinger's cat out of the bag","issue":"2","quality_controlled":"1","language":[{"iso":"eng"}],"oa_version":"None","department":[{"_id":"JiFr"}],"acknowledgement":"Research in J.F. laboratory is funded by the European Union's Horizon 2020 program (ERC grant agreement n° 742985); C.L. is supported by the Austrian Science Fund (FWF grant P 31493).","doi":"10.1016/j.pbi.2019.10.003","date_created":"2019-12-02T12:05:26Z","related_material":{"record":[{"id":"11626","status":"public","relation":"dissertation_contains"}]},"_id":"7142","publication_identifier":{"issn":["1369-5266"],"eissn":["1879-0356"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"02","type":"journal_article","scopus_import":"1","citation":{"apa":"Gallei, M. C., Luschnig, C., &#38; Friml, J. (2020). Auxin signalling in growth: Schrödinger’s cat out of the bag. <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">https://doi.org/10.1016/j.pbi.2019.10.003</a>","ista":"Gallei MC, Luschnig C, Friml J. 2020. Auxin signalling in growth: Schrödinger’s cat out of the bag. Current Opinion in Plant Biology. 53(2), 43–49.","chicago":"Gallei, Michelle C, Christian Luschnig, and Jiří Friml. “Auxin Signalling in Growth: Schrödinger’s Cat out of the Bag.” <i>Current Opinion in Plant Biology</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">https://doi.org/10.1016/j.pbi.2019.10.003</a>.","ama":"Gallei MC, Luschnig C, Friml J. Auxin signalling in growth: Schrödinger’s cat out of the bag. <i>Current Opinion in Plant Biology</i>. 2020;53(2):43-49. doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">10.1016/j.pbi.2019.10.003</a>","mla":"Gallei, Michelle C., et al. “Auxin Signalling in Growth: Schrödinger’s Cat out of the Bag.” <i>Current Opinion in Plant Biology</i>, vol. 53, no. 2, Elsevier, 2020, pp. 43–49, doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">10.1016/j.pbi.2019.10.003</a>.","ieee":"M. C. Gallei, C. Luschnig, and J. Friml, “Auxin signalling in growth: Schrödinger’s cat out of the bag,” <i>Current Opinion in Plant Biology</i>, vol. 53, no. 2. Elsevier, pp. 43–49, 2020.","short":"M.C. Gallei, C. Luschnig, J. Friml, Current Opinion in Plant Biology 53 (2020) 43–49."},"intvolume":"        53","isi":1,"corr_author":"1","date_published":"2020-02-01T00:00:00Z","author":[{"id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368","last_name":"Gallei","first_name":"Michelle C"},{"full_name":"Luschnig, Christian","last_name":"Luschnig","first_name":"Christian"},{"first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"year":"2020","day":"01","status":"public","publication_status":"published","pmid":1,"date_updated":"2026-04-07T14:18:57Z","article_type":"original","publisher":"Elsevier"},{"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"3","oa_version":"Published Version","oa":1,"department":[{"_id":"JiFr"}],"publication_identifier":{"eissn":["2198-3844"]},"_id":"7204","doi":"10.1002/advs.201901455","date_created":"2019-12-22T23:00:43Z","type":"journal_article","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"02","citation":{"chicago":"Li, Yang, Yaping Wang, Shutang Tan, Zhen Li, Zhi Yuan, Matous Glanc, David Domjan, et al. “Root Growth Adaptation Is Mediated by PYLs ABA Receptor-PP2A Protein Phosphatase Complex.” <i>Advanced Science</i>. Wiley, 2020. <a href=\"https://doi.org/10.1002/advs.201901455\">https://doi.org/10.1002/advs.201901455</a>.","ama":"Li Y, Wang Y, Tan S, et al. Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase complex. <i>Advanced Science</i>. 2020;7(3). doi:<a href=\"https://doi.org/10.1002/advs.201901455\">10.1002/advs.201901455</a>","ieee":"Y. Li <i>et al.</i>, “Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase complex,” <i>Advanced Science</i>, vol. 7, no. 3. Wiley, 2020.","mla":"Li, Yang, et al. “Root Growth Adaptation Is Mediated by PYLs ABA Receptor-PP2A Protein Phosphatase Complex.” <i>Advanced Science</i>, vol. 7, no. 3, 1901455, Wiley, 2020, doi:<a href=\"https://doi.org/10.1002/advs.201901455\">10.1002/advs.201901455</a>.","short":"Y. Li, Y. Wang, S. Tan, Z. Li, Z. Yuan, M. Glanc, D. Domjan, K. Wang, W. Xuan, Y. Guo, Z. Gong, J. Friml, J. Zhang, Advanced Science 7 (2020).","apa":"Li, Y., Wang, Y., Tan, S., Li, Z., Yuan, Z., Glanc, M., … Zhang, J. (2020). Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase complex. <i>Advanced Science</i>. Wiley. <a href=\"https://doi.org/10.1002/advs.201901455\">https://doi.org/10.1002/advs.201901455</a>","ista":"Li Y, Wang Y, Tan S, Li Z, Yuan Z, Glanc M, Domjan D, Wang K, Xuan W, Guo Y, Gong Z, Friml J, Zhang J. 2020. Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase complex. Advanced Science. 7(3), 1901455."},"scopus_import":"1","external_id":{"isi":["000501912800001"],"pmid":["32042554"]},"article_processing_charge":"No","volume":7,"abstract":[{"lang":"eng","text":"Plant root architecture dynamically adapts to various environmental conditions, such as salt‐containing soil. The phytohormone abscisic acid (ABA) is involved among others also in these developmental adaptations, but the underlying molecular mechanism remains elusive. Here, a novel branch of the ABA signaling pathway in Arabidopsis involving PYR/PYL/RCAR (abbreviated as PYLs) receptor‐protein phosphatase 2A (PP2A) complex that acts in parallel to the canonical PYLs‐protein phosphatase 2C (PP2C) mechanism is identified. The PYLs‐PP2A signaling modulates root gravitropism and lateral root formation through regulating phytohormone auxin transport. In optimal conditions, PYLs ABA receptor interacts with the catalytic subunits of PP2A, increasing their phosphatase activity and thus counteracting PINOID (PID) kinase‐mediated phosphorylation of PIN‐FORMED (PIN) auxin transporters. By contrast, in salt and osmotic stress conditions, ABA binds to PYLs, inhibiting the PP2A activity, which leads to increased PIN phosphorylation and consequently modulated directional auxin transport leading to adapted root architecture. This work reveals an adaptive mechanism that may flexibly adjust plant root growth to withstand saline and osmotic stresses. It occurs via the cross‐talk between the stress hormone ABA and the versatile developmental regulator auxin."}],"publication":"Advanced Science","file_date_updated":"2020-07-14T12:47:53Z","title":"Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase complex","article_number":"1901455","year":"2020","day":"05","file":[{"file_id":"7519","file_name":"2020_AdvScience_Li.pdf","access_level":"open_access","checksum":"016eeab5860860af038e2da95ffe75c3","date_created":"2020-02-24T14:29:54Z","relation":"main_file","content_type":"application/pdf","date_updated":"2020-07-14T12:47:53Z","file_size":3586924,"creator":"dernst"}],"publication_status":"published","pmid":1,"status":"public","date_updated":"2023-08-17T14:13:17Z","article_type":"original","publisher":"Wiley","intvolume":"         7","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","date_published":"2020-02-05T00:00:00Z","ddc":["580"],"author":[{"full_name":"Li, Yang","last_name":"Li","first_name":"Yang"},{"full_name":"Wang, Yaping","first_name":"Yaping","last_name":"Wang"},{"last_name":"Tan","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285"},{"full_name":"Li, Zhen","last_name":"Li","first_name":"Zhen"},{"first_name":"Zhi","last_name":"Yuan","full_name":"Yuan, Zhi"},{"id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","full_name":"Glanc, Matous","orcid":"0000-0003-0619-7783","last_name":"Glanc","first_name":"Matous"},{"first_name":"David","last_name":"Domjan","full_name":"Domjan, David","id":"C684CD7A-257E-11EA-9B6F-D8588B4F947F","orcid":"0000-0003-2267-106X"},{"full_name":"Wang, Kai","first_name":"Kai","last_name":"Wang"},{"last_name":"Xuan","first_name":"Wei","full_name":"Xuan, Wei"},{"first_name":"Yan","last_name":"Guo","full_name":"Guo, Yan"},{"first_name":"Zhizhong","last_name":"Gong","full_name":"Gong, Zhizhong"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"},{"first_name":"Jing","last_name":"Zhang","full_name":"Zhang, Jing"}]},{"citation":{"ista":"Xiao G, Zhang Y. 2020. Adaptive growth: Shaping auxin-mediated root system architecture. Trends in Plant Science. 25(2), P121-123.","apa":"Xiao, G., &#38; Zhang, Y. (2020). Adaptive growth: Shaping auxin-mediated root system architecture. <i>Trends in Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.tplants.2019.12.001\">https://doi.org/10.1016/j.tplants.2019.12.001</a>","short":"G. Xiao, Y. Zhang, Trends in Plant Science 25 (2020) P121-123.","mla":"Xiao, Guanghui, and Yuzhou Zhang. “Adaptive Growth: Shaping Auxin-Mediated Root System Architecture.” <i>Trends in Plant Science</i>, vol. 25, no. 2, Elsevier, 2020, pp. P121-123, doi:<a href=\"https://doi.org/10.1016/j.tplants.2019.12.001\">10.1016/j.tplants.2019.12.001</a>.","ieee":"G. Xiao and Y. Zhang, “Adaptive growth: Shaping auxin-mediated root system architecture,” <i>Trends in Plant Science</i>, vol. 25, no. 2. Elsevier, pp. P121-123, 2020.","ama":"Xiao G, Zhang Y. Adaptive growth: Shaping auxin-mediated root system architecture. <i>Trends in Plant Science</i>. 2020;25(2):P121-123. doi:<a href=\"https://doi.org/10.1016/j.tplants.2019.12.001\">10.1016/j.tplants.2019.12.001</a>","chicago":"Xiao, Guanghui, and Yuzhou Zhang. “Adaptive Growth: Shaping Auxin-Mediated Root System Architecture.” <i>Trends in Plant Science</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.tplants.2019.12.001\">https://doi.org/10.1016/j.tplants.2019.12.001</a>."},"scopus_import":"1","type":"journal_article","month":"02","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"7219","publication_identifier":{"issn":["1360-1385"]},"date_created":"2019-12-29T23:00:48Z","doi":"10.1016/j.tplants.2019.12.001","department":[{"_id":"JiFr"}],"oa_version":"None","language":[{"iso":"eng"}],"quality_controlled":"1","issue":"2","title":"Adaptive growth: Shaping auxin-mediated root system architecture","volume":25,"publication":"Trends in Plant Science","abstract":[{"text":"Root system architecture (RSA), governed by the phytohormone auxin, endows plants with an adaptive advantage in particular environments. Using geographically representative arabidopsis (Arabidopsis thaliana) accessions as a resource for GWA mapping, Waidmann et al. and Ogura et al. recently identified two novel components involved in modulating auxin-mediated RSA and conferring plant fitness in particular habitats.","lang":"eng"}],"page":"P121-123","external_id":{"isi":["000508637500001"],"pmid":["31843370"]},"article_processing_charge":"No","publisher":"Elsevier","article_type":"original","date_updated":"2025-07-10T11:54:24Z","pmid":1,"publication_status":"published","status":"public","day":"01","year":"2020","author":[{"full_name":"Xiao, Guanghui","first_name":"Guanghui","last_name":"Xiao"},{"first_name":"Yuzhou","last_name":"Zhang","full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956"}],"date_published":"2020-02-01T00:00:00Z","corr_author":"1","isi":1,"intvolume":"        25"},{"article_processing_charge":"No","external_id":{"pmid":["31696764"],"isi":["000494909300001"]},"abstract":[{"text":"Earlier, we demonstrated that transcript levels of METAL TOLERANCE PROTEIN2 (MTP2) and of HEAVY METAL ATPase2 (HMA2) increase strongly in roots of Arabidopsis upon prolonged zinc (Zn) deficiency and respond to shoot physiological Zn status, and not to the local Zn status in roots. This provided evidence for shoot-to-root communication in the acclimation of plants to Zn deficiency. Zn-deficient soils limit both the yield and quality of agricultural crops and can result in clinically relevant nutritional Zn deficiency in human populations. Implementing Zn deficiency during cultivation of the model plant Arabidopsis thaliana on agar-solidified media is difficult because trace element contaminations are present in almost all commercially available agars. Here, we demonstrate root morphological acclimations to Zn deficiency on agar-solidified medium following the effective removal of contaminants. These advancements allow reproducible phenotyping toward understanding fundamental plant responses to deficiencies of Zn and other essential trace elements.","lang":"eng"}],"publication":"Plant Signaling & Behavior","volume":15,"title":"Generation of effective zinc-deficient agar-solidified media allows identification of root morphology changes in response to zinc limitation","article_number":"1687175","issue":"1","language":[{"iso":"eng"}],"quality_controlled":"1","department":[{"_id":"JiFr"}],"oa_version":"Submitted Version","oa":1,"doi":"10.1080/15592324.2019.1687175","date_created":"2020-01-30T10:12:04Z","_id":"7416","publication_identifier":{"issn":["1559-2324"]},"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7012054"}],"scopus_import":"1","citation":{"apa":"Sinclair, S. A., &#38; Krämer, U. (2020). Generation of effective zinc-deficient agar-solidified media allows identification of root morphology changes in response to zinc limitation. <i>Plant Signaling &#38; Behavior</i>. Taylor &#38; Francis. <a href=\"https://doi.org/10.1080/15592324.2019.1687175\">https://doi.org/10.1080/15592324.2019.1687175</a>","ista":"Sinclair SA, Krämer U. 2020. Generation of effective zinc-deficient agar-solidified media allows identification of root morphology changes in response to zinc limitation. Plant Signaling &#38; Behavior. 15(1), 1687175.","ama":"Sinclair SA, Krämer U. Generation of effective zinc-deficient agar-solidified media allows identification of root morphology changes in response to zinc limitation. <i>Plant Signaling &#38; Behavior</i>. 2020;15(1). doi:<a href=\"https://doi.org/10.1080/15592324.2019.1687175\">10.1080/15592324.2019.1687175</a>","chicago":"Sinclair, Scott A, and U. Krämer. “Generation of Effective Zinc-Deficient Agar-Solidified Media Allows Identification of Root Morphology Changes in Response to Zinc Limitation.” <i>Plant Signaling &#38; Behavior</i>. Taylor &#38; Francis, 2020. <a href=\"https://doi.org/10.1080/15592324.2019.1687175\">https://doi.org/10.1080/15592324.2019.1687175</a>.","short":"S.A. Sinclair, U. Krämer, Plant Signaling &#38; Behavior 15 (2020).","ieee":"S. A. Sinclair and U. Krämer, “Generation of effective zinc-deficient agar-solidified media allows identification of root morphology changes in response to zinc limitation,” <i>Plant Signaling &#38; Behavior</i>, vol. 15, no. 1. Taylor &#38; Francis, 2020.","mla":"Sinclair, Scott A., and U. Krämer. “Generation of Effective Zinc-Deficient Agar-Solidified Media Allows Identification of Root Morphology Changes in Response to Zinc Limitation.” <i>Plant Signaling &#38; Behavior</i>, vol. 15, no. 1, 1687175, Taylor &#38; Francis, 2020, doi:<a href=\"https://doi.org/10.1080/15592324.2019.1687175\">10.1080/15592324.2019.1687175</a>."},"month":"01","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","isi":1,"intvolume":"        15","date_published":"2020-01-01T00:00:00Z","author":[{"first_name":"Scott A","last_name":"Sinclair","full_name":"Sinclair, Scott A","id":"2D99FE6A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4566-0593"},{"last_name":"Krämer","first_name":"U.","full_name":"Krämer, U."}],"day":"01","year":"2020","status":"public","publication_status":"published","pmid":1,"article_type":"original","publisher":"Taylor & Francis","date_updated":"2023-10-17T09:01:48Z"}]