[{"title":"Role of cAMP in TIR1/AFB auxin signaling: Open issues","intvolume":"        31","has_accepted_license":"1","year":"2026","abstract":[{"text":"The canonical mechanism by which the phytohormone auxin regulates transcription has been one of the cornerstones of plant signaling. The recent unexpected discovery of cyclic AMP (cAMP) as a second messenger in this pathway has revised its foundations while leaving many open questions and gaps in our understanding; these will be discussed in this forum article.","lang":"eng"}],"researchdata_availability":"no","date_updated":"2026-10-20T09:01:46Z","month":"02","file":[{"creator":"dernst","relation":"main_file","success":1,"file_size":432792,"date_created":"2026-07-27T08:26:40Z","checksum":"e60e903fb4b3e917dba763976ec652cd","content_type":"application/pdf","file_id":"22413","access_level":"open_access","date_updated":"2026-07-27T08:26:40Z","file_name":"2026_TrendsPlantScience_Friml.pdf"}],"publication":"Trends in Plant Science","supplementarymaterial":"no","das_tickbox":"0","issue":"2","acknowledgement":"I apologize to colleagues whose relevant work I was unable to cite due to space limitations. This work was funded by the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; 37051-B). I thank Drs Huihuang Chen, Yuanrong Pei, Jason Reed, Linlin Qi, and Dolf Weijers for inspiration and critical input.","OA_type":"hybrid","article_processing_charge":"Yes (via OA deal)","pmid":1,"type":"journal_article","department":[{"_id":"JiFr"}],"doi":"10.1016/j.tplants.2025.10.018","file_date_updated":"2026-07-27T08:26:40Z","external_id":{"pmid":["41249070"]},"publication_identifier":{"eissn":["1878-4372"],"issn":["1360-1385"]},"date_published":"2026-02-01T00:00:00Z","article_type":"review","PlanS_conform":"1","publisher":"Cell Press","status":"public","author":[{"full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"OA_place":"publisher","page":"136-138","citation":{"ama":"Friml J. Role of cAMP in TIR1/AFB auxin signaling: Open issues. <i>Trends in Plant Science</i>. 2026;31(2):136-138. doi:<a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">10.1016/j.tplants.2025.10.018</a>","mla":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” <i>Trends in Plant Science</i>, vol. 31, no. 2, Cell Press, 2026, pp. 136–38, doi:<a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">10.1016/j.tplants.2025.10.018</a>.","ista":"Friml J. 2026. Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. 31(2), 136–138.","ieee":"J. Friml, “Role of cAMP in TIR1/AFB auxin signaling: Open issues,” <i>Trends in Plant Science</i>, vol. 31, no. 2. Cell Press, pp. 136–138, 2026.","short":"J. Friml, Trends in Plant Science 31 (2026) 136–138.","chicago":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” <i>Trends in Plant Science</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">https://doi.org/10.1016/j.tplants.2025.10.018</a>.","apa":"Friml, J. (2026). Role of cAMP in TIR1/AFB auxin signaling: Open issues. <i>Trends in Plant Science</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">https://doi.org/10.1016/j.tplants.2025.10.018</a>"},"quality_controlled":"1","language":[{"iso":"eng"}],"oa_version":"Published Version","corr_author":"1","scopus_import":"1","_id":"20725","ddc":["580"],"day":"01","date_created":"2025-12-02T16:29:22Z","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"volume":31,"oa":1,"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1016/j.tplants.2025.10.018"},{"publication_status":"published","dataavailabilitystatement":"Plasmids and genetic materials generated in this study will be made available upon request from the lead contact.The mass spectrometry data reported in this work have been deposited at the iProX database. Original western blot gel images have been deposited to Mendeley Data. The accession code and the DOI are listed in the key resources table.\r\nThis paper does not report original code. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","volume":61,"fulldoi":"https://doi.org/10.1016/j.devcel.2025.09.009","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"20636","scopus_import":"1","oa_version":"None","language":[{"iso":"eng"}],"day":"14","date_created":"2025-11-12T10:03:39Z","status":"public","publisher":"Cell Press","quality_controlled":"1","citation":{"mla":"Huang, R., et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” <i>Developmental Cell</i>, vol. 61, no. 1, Cell Press, 2026, pp. 73–84, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">10.1016/j.devcel.2025.09.009</a>.","ista":"Huang R, Wang J, Chang M, Tang W, Yu Y, Zhang Y, Peng Y, Wang Y, Guo Y, Lu T, Cao Y, Zhou Y, Zhang Q, Huang Y, Wu A, Ren L, Gallei MC, Dong J, Chen H, He J, Wen M, Friml J, Sun L, Xiong Y, Yang Z, Xu T. 2026. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. 61(1), 73–84.","ama":"Huang R, Wang J, Chang M, et al. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. <i>Developmental Cell</i>. 2026;61(1):73-84. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">10.1016/j.devcel.2025.09.009</a>","chicago":"Huang, R, J Wang, M Chang, W Tang, Y Yu, Y Zhang, Y Peng, et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” <i>Developmental Cell</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">https://doi.org/10.1016/j.devcel.2025.09.009</a>.","apa":"Huang, R., Wang, J., Chang, M., Tang, W., Yu, Y., Zhang, Y., … Xu, T. (2026). TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">https://doi.org/10.1016/j.devcel.2025.09.009</a>","ieee":"R. Huang <i>et al.</i>, “TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis,” <i>Developmental Cell</i>, vol. 61, no. 1. Cell Press, pp. 73–84, 2026.","short":"R. Huang, J. Wang, M. Chang, W. Tang, Y. Yu, Y. Zhang, Y. Peng, Y. Wang, Y. Guo, T. Lu, Y. Cao, Y. Zhou, Q. Zhang, Y. Huang, A. Wu, L. Ren, M.C. Gallei, J. Dong, H. Chen, J. He, M. Wen, J. Friml, L. Sun, Y. Xiong, Z. Yang, T. Xu, Developmental Cell 61 (2026) 73–84."},"page":"73-84","author":[{"last_name":"Huang","first_name":"R","full_name":"Huang, R"},{"first_name":"J","full_name":"Wang, J","last_name":"Wang"},{"full_name":"Chang, M","first_name":"M","last_name":"Chang"},{"last_name":"Tang","full_name":"Tang, W","first_name":"W"},{"full_name":"Yu, Y","first_name":"Y","last_name":"Yu"},{"last_name":"Zhang","full_name":"Zhang, Y","first_name":"Y"},{"first_name":"Y","full_name":"Peng, Y","last_name":"Peng"},{"last_name":"Wang","full_name":"Wang, Y","first_name":"Y"},{"full_name":"Guo, Y","first_name":"Y","last_name":"Guo"},{"last_name":"Lu","full_name":"Lu, T","first_name":"T"},{"first_name":"Y","full_name":"Cao, Y","last_name":"Cao"},{"last_name":"Zhou","full_name":"Zhou, Y","first_name":"Y"},{"full_name":"Zhang, Q","first_name":"Q","last_name":"Zhang"},{"last_name":"Huang","first_name":"Y","full_name":"Huang, Y"},{"first_name":"A","full_name":"Wu, A","last_name":"Wu"},{"last_name":"Ren","full_name":"Ren, L","first_name":"L"},{"full_name":"Gallei, Michelle C","first_name":"Michelle C","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1286-7368"},{"last_name":"Dong","first_name":"J","full_name":"Dong, J"},{"last_name":"Chen","first_name":"H","full_name":"Chen, H"},{"full_name":"He, J","first_name":"J","last_name":"He"},{"last_name":"Wen","full_name":"Wen, M","first_name":"M"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří"},{"full_name":"Sun, L","first_name":"L","last_name":"Sun"},{"first_name":"Y","full_name":"Xiong, Y","last_name":"Xiong"},{"full_name":"Yang, Z","first_name":"Z","last_name":"Yang"},{"first_name":"T","full_name":"Xu, T","last_name":"Xu"}],"doi":"10.1016/j.devcel.2025.09.009","department":[{"_id":"JiFr"}],"article_type":"original","date_published":"2026-01-14T00:00:00Z","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"external_id":{"pmid":["41043435"]},"article_processing_charge":"No","OA_type":"closed access","type":"journal_article","pmid":1,"das_tickbox":"1","supplementarymaterial":"yes","acknowledgement":"We thank Lukáš Fiedler‬ for helping with the writing. This work was supported by the National Key Research and Development Program of China (2023YFA0913500) to T.X., R.H., Y.Y., Y.X., and M.W. and by the National Natural Science Foundation of China grants to T.X. (32130010), Z.Y. (3241101698), and R.H. (32070309 and 32470276) and startup funds from the Fujian Agriculture and Forestry University and the Shanghai Plant Stress Biology Center, Chinese Academy of Sciences to T.X.","issue":"1","publication":"Developmental Cell","month":"01","researchdata_availability":"upon request","date_updated":"2026-10-20T09:02:01Z","title":"TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis","intvolume":"        61","abstract":[{"lang":"eng","text":"The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in Arabidopsis."}],"year":"2026"},{"pmid":1,"type":"journal_article","OA_type":"green","article_processing_charge":"No","issue":"6795","acknowledgement":"We gratefully acknowledge the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) (both of ISTA) and the Hounsfield CT Facility (University of Nottingham) for support with imaging and the Growth Facility (IPMB) for plant cultivation. We thank M. Fendrych and his team for help with the microfluidics upgrades and J. Atkinson at the University of Nottingham MakerSpace for 3D printing of Arabidopsis mini-soil columns.\r\nThis project received funding from the European Research Council (ERC; 101142681 CYNIPS) and the Austrian Science Fund (FWF; P 37051-B). I.K. was cofunded by the European Union, Horizon Europe, project MOLIPEC, ID 101087030 and CSF project 25-16449S. L.V. and B.K.P. acknowledge funding from UK Research and Innovation (UKRI) Frontiers Research (EP/Y036697/1). M.J.B. acknowledges funding from ERC SYNERGY (grant 101118769 HYDROSENSING). The study was partially supported by the Université Paris Cité, Idex ANR-18-IDEX-0001, funded by the French Government through its “Investments for the Future” program and also by the projects “Mecha-Nuc” ANR-20-CE13-0025-03 and “scEm-bryoMech” ANR-21-CE13-0046. P.D. acknowledges support by Human Frontier Science Program Organization grant 2022-RG107. P.V. acknowledges support provided by “Programme blanc” of the Graduate School BIOSPHERA, Université Paris-Saclay. Phytohormonal analysis was performed using the service laboratory funded by Toward Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the European Regional Development Fund (ERDF) program Johannes Amos Comenius. This research was funded in whole or in part by the Austrian Science Fund (P 37051-B) and UK Research and Innovation (EP/Y036697/1), cOAlition S organizations, and by the European Research Council (101142681 CYNIPS, 101118769 HYDROSENSING); as required, the author will make the Author Accepted Manuscript (AAM) version available under a CC BY public copyright license.","date_updated":"2026-10-22T09:15:44Z","month":"04","file":[{"success":1,"relation":"main_file","creator":"dernst","checksum":"eb5b29247832ecdc53c8146da0509bbe","content_type":"application/pdf","file_size":6150733,"date_created":"2026-05-07T05:54:43Z","file_id":"21832","access_level":"open_access","date_updated":"2026-05-07T05:54:43Z","file_name":"2026_Science_Kulich_accepted.pdf"}],"publication":"Science","year":"2026","abstract":[{"text":"Reactive oxygen species (ROS) have been implicated in multiple signaling processes in plants, but the underlying mechanisms and roles remain enigmatic. In this study, we developed a method of live imaging of apoplastic ROS at the root surface. Distinct signals, including auxin, extracellular adenosine triphosphate, and rapid alkalinization factor 1 peptide, induce cytosolic calcium transients and apoplastic ROS bursts. Genetic and optogenetic manipulations of Arabidopsis identified calcium transients as necessary and sufficient for ROS bursts through activation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases RBOHC and RBOHF. Apoplastic ROS bursts are not required, but they do limit gravity-induced root bending. Root bending is sensed by the stretch-activated calcium channel MCA1, leading to NADPH oxidase activation. The resulting ROS production stiffens cell walls to facilitate soil penetration. Apoplastic ROS thus provides a means to balance tissue flexibility and stiffness to navigate soil.","lang":"eng"}],"related_material":{"record":[{"id":"23145","status":"public","relation":"dissertation_contains"}]},"intvolume":"       392","title":"Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation","has_accepted_license":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1126/science.adu8197","project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"volume":392,"publication_status":"published","oa":1,"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"ddc":["580"],"day":"16","date_created":"2026-04-26T22:01:47Z","corr_author":"1","oa_version":"Accepted Version","language":[{"iso":"eng"}],"scopus_import":"1","_id":"21763","OA_place":"repository","page":"296-300","author":[{"last_name":"Kulich","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","full_name":"Kulich, Ivan","first_name":"Ivan"},{"first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev"},{"full_name":"Randuch, Marek","first_name":"Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"last_name":"Gao","full_name":"Gao, Shiqiang","first_name":"Shiqiang"},{"full_name":"Citterico, Matteo","first_name":"Matteo","last_name":"Citterico"},{"full_name":"Konrad, Kai R.","first_name":"Kai R.","last_name":"Konrad"},{"full_name":"Nagel, Georg","first_name":"Georg","last_name":"Nagel"},{"full_name":"Wrzaczek, Michael","first_name":"Michael","last_name":"Wrzaczek"},{"first_name":"Léa","full_name":"Cascaro, Léa","last_name":"Cascaro"},{"last_name":"Vinet","first_name":"Pauline","full_name":"Vinet, Pauline"},{"full_name":"Durand, Pauline","first_name":"Pauline","last_name":"Durand"},{"last_name":"Asnacios","full_name":"Asnacios, Atef","first_name":"Atef"},{"last_name":"Verma","first_name":"Lokesh","full_name":"Verma, Lokesh"},{"last_name":"Bennett","first_name":"Malcolm J.","full_name":"Bennett, Malcolm J."},{"full_name":"Pandey, Bipin K.","first_name":"Bipin K.","last_name":"Pandey"},{"first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"citation":{"apa":"Kulich, I., Vladimirtsev, D., Randuch, M., Gao, S., Citterico, M., Konrad, K. R., … Friml, J. (2026). Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>","chicago":"Kulich, Ivan, Dmitrii Vladimirtsev, Marek Randuch, Shiqiang Gao, Matteo Citterico, Kai R. Konrad, Georg Nagel, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>.","short":"I. Kulich, D. Vladimirtsev, M. Randuch, S. Gao, M. Citterico, K.R. Konrad, G. Nagel, M. Wrzaczek, L. Cascaro, P. Vinet, P. Durand, A. Asnacios, L. Verma, M.J. Bennett, B.K. Pandey, J. Friml, Science 392 (2026) 296–300.","ieee":"I. Kulich <i>et al.</i>, “Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation,” <i>Science</i>, vol. 392, no. 6795. AAAS, pp. 296–300, 2026.","ista":"Kulich I, Vladimirtsev D, Randuch M, Gao S, Citterico M, Konrad KR, Nagel G, Wrzaczek M, Cascaro L, Vinet P, Durand P, Asnacios A, Verma L, Bennett MJ, Pandey BK, Friml J. 2026. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 392(6795), 296–300.","mla":"Kulich, Ivan, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>, vol. 392, no. 6795, AAAS, 2026, pp. 296–300, doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>.","ama":"Kulich I, Vladimirtsev D, Randuch M, et al. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. 2026;392(6795):296-300. doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>"},"quality_controlled":"1","publisher":"AAAS","status":"public","external_id":{"pmid":["41990180"]},"publication_identifier":{"eissn":["1095-9203"],"issn":["0036-8075"]},"date_published":"2026-04-16T00:00:00Z","article_type":"original","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"doi":"10.1126/science.adu8197","file_date_updated":"2026-05-07T05:54:43Z"},{"alternative_title":["ISTA Thesis"],"date_updated":"2026-10-22T09:15:44Z","month":"10","file":[{"creator":"mranduch","relation":"source_file","file_size":89544771,"date_created":"2026-10-20T09:17:28Z","checksum":"4ace20ba4f1bb0f8dcc3f8eb45eb68fa","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_id":"23176","access_level":"closed","date_updated":"2026-10-20T11:48:37Z","file_name":"2026_Marek_Randuch_Thesis.docx"},{"file_name":"2026_Marek_Randuch_Thesis.pdf","date_updated":"2026-10-20T11:48:37Z","access_level":"closed","file_id":"23177","embargo":"2027-10-20","content_type":"application/pdf","checksum":"fc630c7e8d795e7f13000cbb428424b3","embargo_to":"open_access","date_created":"2026-10-20T09:17:20Z","file_size":28321059,"relation":"main_file","creator":"mranduch"}],"year":"2026","title":"Fast growth responses in plants: Growth regulation and phospho-proteomic ","has_accepted_license":"1","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"12291"},{"id":"21763","relation":"part_of_dissertation","status":"public"},{"id":"23175","status":"public","relation":"part_of_dissertation"},{"relation":"part_of_dissertation","status":"public","id":"23174"},{"id":"20656","relation":"part_of_dissertation","status":"public"}]},"type":"dissertation","article_processing_charge":"No","acknowledgement":"European Research Council (101142681 CYNIPS), Cyclic nucleotides as second messengers \r\nin plants, DOI: https://doi.org/10.3030/101142681 \r\nAustrian Science Fund (P 37051-B), Guanylate cyclase activity of TIR1/AFBs auxin receptors, \r\nDOI: https://doi.org/10.55776/P37051 ","supervisor":[{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří"}],"degree_awarded":"PhD","citation":{"apa":"Randuch, M. (2026). <i>Fast growth responses in plants: Growth regulation and phospho-proteomic </i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-23145\">https://doi.org/10.15479/AT-ISTA-23145</a>","chicago":"Randuch, Marek. “Fast Growth Responses in Plants: Growth Regulation and Phospho-Proteomic .” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-23145\">https://doi.org/10.15479/AT-ISTA-23145</a>.","short":"M. Randuch, Fast Growth Responses in Plants: Growth Regulation and Phospho-Proteomic , Institute of Science and Technology Austria, 2026.","ieee":"M. Randuch, “Fast growth responses in plants: Growth regulation and phospho-proteomic ,” Institute of Science and Technology Austria, 2026.","ista":"Randuch M. 2026. Fast growth responses in plants: Growth regulation and phospho-proteomic . Institute of Science and Technology Austria.","mla":"Randuch, Marek. <i>Fast Growth Responses in Plants: Growth Regulation and Phospho-Proteomic </i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-23145\">10.15479/AT-ISTA-23145</a>.","ama":"Randuch M. Fast growth responses in plants: Growth regulation and phospho-proteomic . 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-23145\">10.15479/AT-ISTA-23145</a>"},"OA_place":"publisher","page":"289","author":[{"full_name":"Randuch, Marek","first_name":"Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"}],"status":"public","publisher":"Institute of Science and Technology Austria","publication_identifier":{"issn":["2663-337X"]},"date_published":"2026-10-18T00:00:00Z","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"file_date_updated":"2026-10-20T11:48:37Z","doi":"10.15479/AT-ISTA-23145","fulldoi":"https://doi.org/10.15479/AT-ISTA-23145","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","publication_status":"published","project":[{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"},{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681"}],"tmp":{"short":"CC BY-SA (4.0)","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","image":"/images/cc_by_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode"},"date_created":"2026-10-16T17:14:40Z","day":"18","doi_confirm":"1","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"CampIT"},{"_id":"Bio"}],"ddc":["580"],"_id":"23145","language":[{"iso":"eng"}],"corr_author":"1","oa_version":"Published Version"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1101/2025.10.04.680446","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"project":[{"grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051"}],"oa":1,"publication_status":"draft","day":"26","date_created":"2026-10-19T11:51:47Z","language":[{"iso":"eng"}],"corr_author":"1","oa_version":"Preprint","_id":"23175","OA_place":"repository","author":[{"first_name":"Huifang","full_name":"Song, Huifang","last_name":"Song"},{"full_name":"Baudon, Angel","first_name":"Angel","last_name":"Baudon"},{"last_name":"Freund","first_name":"Matthias","full_name":"Freund, Matthias"},{"last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","full_name":"Randuch, Marek","first_name":"Marek"},{"last_name":"Pěnčík","full_name":"Pěnčík, Aleš","first_name":"Aleš"},{"last_name":"Ondřej","first_name":"Novák","full_name":"Ondřej, Novák"},{"full_name":"He, Zhaohui","first_name":"Zhaohui","last_name":"He"},{"last_name":"Kaufmann","full_name":"Kaufmann, Kerstin","first_name":"Kerstin"},{"last_name":"Li","full_name":"Li, Nan","first_name":"Nan"},{"last_name":"Gilliham","full_name":"Gilliham, Matthew","first_name":"Matthew"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří"},{"last_name":"Hedrich","full_name":"Hedrich, Rainer","first_name":"Rainer"},{"first_name":"Shouguang","full_name":"Huang, Shouguang","last_name":"Huang"}],"citation":{"ista":"Song H, Baudon A, Freund M, Randuch M, Pěnčík A, Ondřej N, He Z, Kaufmann K, Li N, Gilliham M, Friml J, Hedrich R, Huang S. Ca2+ signature gates early auxin signaling in Arabidopsis roots. bioRxiv, <a href=\"https://doi.org/10.1101/2025.10.04.680446\">10.1101/2025.10.04.680446</a>.","mla":"Song, Huifang, et al. “Ca2+ Signature Gates Early Auxin Signaling in Arabidopsis Roots.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.10.04.680446\">10.1101/2025.10.04.680446</a>.","ama":"Song H, Baudon A, Freund M, et al. Ca2+ signature gates early auxin signaling in Arabidopsis roots. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.10.04.680446\">10.1101/2025.10.04.680446</a>","apa":"Song, H., Baudon, A., Freund, M., Randuch, M., Pěnčík, A., Ondřej, N., … Huang, S. (n.d.). Ca2+ signature gates early auxin signaling in Arabidopsis roots. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.10.04.680446\">https://doi.org/10.1101/2025.10.04.680446</a>","chicago":"Song, Huifang, Angel Baudon, Matthias Freund, Marek Randuch, Aleš Pěnčík, Novák Ondřej, Zhaohui He, et al. “Ca2+ Signature Gates Early Auxin Signaling in Arabidopsis Roots.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.10.04.680446\">https://doi.org/10.1101/2025.10.04.680446</a>.","short":"H. Song, A. Baudon, M. Freund, M. Randuch, A. Pěnčík, N. Ondřej, Z. He, K. Kaufmann, N. Li, M. Gilliham, J. Friml, R. Hedrich, S. Huang, BioRxiv (n.d.).","ieee":"H. Song <i>et al.</i>, “Ca2+ signature gates early auxin signaling in Arabidopsis roots,” <i>bioRxiv</i>. ."},"status":"public","date_published":"2026-08-26T00:00:00Z","external_id":{"biorxivid":["10.1101/2025.10.04.680446"]},"doi":"10.1101/2025.10.04.680446","department":[{"_id":"JiFr"}],"biorxivid":1,"type":"preprint","OA_type":"green","article_processing_charge":"No","acknowledgement":"We would like to thank Dirk Becker (University of Wuerzburg) for his help in making the optogenetic constructs and for his comments on the manuscript. We also appreciate Rob Roelfsema (University of Wuerzburg) for his feedback on the manuscript. We sincerely thank the anonymous reviewers for the thoughtful and inspiring suggestions that helped broaden the discussion and identify important future research directions. Funding\r\nNational Key R&D Program of China, 2025YFA0923400 (RH)\r\n\r\nNational Natural Science Foundation of China (NSFC), W2531024 (RH); 32271501(NL)\r\n\r\nNSFC-Excellent Young Scientists Fund Program (overseas) (SH)\r\n\r\nNSFC-RFIS, ZB2025101410702 (RH)\r\n\r\nGuangdong Pearl River Talents Program, 2023ZT10N033\r\n\r\nChina Scholarship Council no. 202306510063 (HS)\r\n\r\nThe European Research Council grant 101142681 CYNIPS (JF)\r\n\r\nAustrian Science Fund grant FWF; P 37051-B (JF)\r\n\r\nGerman Research Foundation grant CRC 1644 (512328399; 512328399) (KK, ZH)\r\n\r\nERC Synergy project STARMORPH, reg. no. 101166880 (AP, ON)","month":"08","date_updated":"2026-10-22T09:15:44Z","publication":"bioRxiv","year":"2026","abstract":[{"text":"Plants must continually balance growth with arrest, especially under stress. Auxin signaling acts as a central regulatory hub in this process, yet the mechanisms that dynamically tune early auxin signalling in real time remain unknown. Here, we used the light-gated, Ca2+-permeable Channelrhodopsin 2 variant XXM2.0 to optogenetically impose defined Ca2+ signatures on Arabidopsis root cells. Repetitive light activation triggered cytosolic Ca2+ signals that in turn suppressed auxin-induced membrane depolarization and Ca2+ transients. Moreover, prolonged optogenetic Ca2+ stimulation affects auxin-responsive transcriptional reprogramming. As phenotypic output, reversible inhibition of root growth by suppressing cell division and elongation was observed. We further identify a candidate CaM7–CNGC14 module that likely mediates Ca2+-dependent gating of early auxin signalling. Our study thus introduces a synthetic biology approach to decompose calcium–auxin crosstalk in plant cells, and demonstrates that optogenetically imposed cytosolic Ca2+ signals act as dynamic regulators of auxin susceptibility in roots.","lang":"eng"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.10.04.680446"}],"related_material":{"record":[{"id":"23145","status":"public","relation":"dissertation_contains"}]},"title":"Ca2+ signature gates early auxin signaling in Arabidopsis roots"},{"language":[{"iso":"eng"}],"corr_author":"1","oa_version":"Preprint","_id":"20982","acknowledgement":"This project was supported by the Czech Science Foundation grant Nr. 25-16449S and by European\r\nUnion, Horizon Europe, project MOLIPEC, ID 101087030. Computational resources used for structural\r\nmodeling were provided by the e-INFRA CZ project (ID:90254), supported by the Ministry of Education,\r\nYouth and Sports of the Czech Republic. Part of the work was carried out with the support of a Growth\r\nFacility (BC Core Facilities; IPMB BC CAS). X. laevis oocytes were kindly provided by C. Korbmacher on\r\na regular basis (FAU Erlangen-Nürnberg). MF received support from the European Research Council\r\n(Grant 480 No. 101125499). We acknowledge the core facility LMH, the BC CAS supported by the MEYS\r\nCR (LM 2023050 Czech-BioImaging). DO received support from the Czech Science Foundation grant Nr.\r\n24-12107S\r\n","day":"16","date_created":"2026-01-13T14:07:58Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"publication_status":"draft","oa":1,"article_processing_charge":"No","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","type":"preprint","fulldoi":"https://doi.org/10.1101/2025.01.06.631460","department":[{"_id":"JiFr"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"20964"}]},"doi":"10.1101/2025.01.06.631460","title":"Armadillo repeat only proteins are required for the function of plant CNGC channels","date_published":"2025-05-16T00:00:00Z","year":"2025","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.01.06.631460"}],"abstract":[{"lang":"eng","text":"Plant cells respond to a wide range of stimuli through intracellular calcium (Ca2+) signaling. Cyclic nucleotide-gated channels (CNGCs) are a major class of plant Ca2+ channels, with 20 homologs in Arabidopsis. These tetrameric plasma membrane proteins act downstream of diverse signals, such as phytohormones, extracellular damage, cell wall integrity or temperature. Here, we identify a class of plant-specific proteins, Armadillo Repeat Only (ARO), as essential regulators of possibly all plant CNGCs. Abrogation of functional sporophytic AROs results in a phenotypic pattern strongly reminiscent of CNGC dysfunction, including defects in root gravitropism, root hair growth and morphology, stomatal movement, and responses to extracellular ATP and the phytohormone auxin. aro2/3/4 mutants are fully resistant to the toxic effects caused by overexpression of CNGCs. AROs colocalize and physically interact with multiple CNGCs and modulate CNGC-dependent currents in Xenopus oocytes. Structural modeling and site-directed mutagenesis reveal AROs tetramer formation surrounding the CNGC channel, interacting via its IQ domain. Taken together, plant CNGC channels don’t act alone, but in a larger complex - channelosome, first of a kind in plants."}],"status":"public","OA_place":"repository","date_updated":"2026-04-07T11:41:43Z","author":[{"full_name":"Kulich, Ivan","first_name":"Ivan","last_name":"Kulich","id":"57a1567c-8314-11eb-9063-c9ddc3451a54"},{"full_name":"Oulehlová, Denisa","first_name":"Denisa","last_name":"Oulehlová"},{"last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii"},{"last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia","first_name":"Minxia"},{"last_name":"Lileikyte","full_name":"Lileikyte, Edita","first_name":"Edita"},{"first_name":"Alexey","full_name":"Bondar, Alexey","last_name":"Bondar"},{"first_name":"Katarína","full_name":"Kulichová, Katarína","last_name":"Kulichová"},{"last_name":"Janda","full_name":"Janda, Martin","first_name":"Martin"},{"full_name":"Iakovenko, Oksana","first_name":"Oksana","last_name":"Iakovenko"},{"last_name":"Neubergerová","first_name":"Michaela","full_name":"Neubergerová, Michaela"},{"last_name":"Studtrucker","full_name":"Studtrucker, Tanja","first_name":"Tanja"},{"last_name":"Pleskot","full_name":"Pleskot, Roman","first_name":"Roman"},{"last_name":"Dietrich","full_name":"Dietrich, Petra","first_name":"Petra"},{"first_name":"Matyas","full_name":"Fendrych, Matyas","orcid":"0000-0002-9767-8699","id":"43905548-F248-11E8-B48F-1D18A9856A87","last_name":"Fendrych"},{"full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"month":"05","citation":{"apa":"Kulich, I., Oulehlová, D., Vladimirtsev, D., Zou, M., Lileikyte, E., Bondar, A., … Friml, J. (n.d.). Armadillo repeat only proteins are required for the function of plant CNGC channels. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.01.06.631460\">https://doi.org/10.1101/2025.01.06.631460</a>","chicago":"Kulich, Ivan, Denisa Oulehlová, Dmitrii Vladimirtsev, Minxia Zou, Edita Lileikyte, Alexey Bondar, Katarína Kulichová, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.01.06.631460\">https://doi.org/10.1101/2025.01.06.631460</a>.","short":"I. Kulich, D. Oulehlová, D. Vladimirtsev, M. Zou, E. Lileikyte, A. Bondar, K. Kulichová, M. Janda, O. Iakovenko, M. Neubergerová, T. Studtrucker, R. Pleskot, P. Dietrich, M. Fendrych, J. Friml, BioRxiv (n.d.).","ieee":"I. Kulich <i>et al.</i>, “Armadillo repeat only proteins are required for the function of plant CNGC channels,” <i>bioRxiv</i>. .","ista":"Kulich I, Oulehlová D, Vladimirtsev D, Zou M, Lileikyte E, Bondar A, Kulichová K, Janda M, Iakovenko O, Neubergerová M, Studtrucker T, Pleskot R, Dietrich P, Fendrych M, Friml J. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv, <a href=\"https://doi.org/10.1101/2025.01.06.631460\">10.1101/2025.01.06.631460</a>.","mla":"Kulich, Ivan, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.01.06.631460\">10.1101/2025.01.06.631460</a>.","ama":"Kulich I, Oulehlová D, Vladimirtsev D, et al. Armadillo repeat only proteins are required for the function of plant CNGC channels. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.01.06.631460\">10.1101/2025.01.06.631460</a>"},"publication":"bioRxiv"},{"status":"public","publisher":"Springer Nature","quality_controlled":"1","citation":{"apa":"Monzer, A., &#38; Friml, J. (2025). Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. <i>Npj Science of Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s44383-025-00002-8\">https://doi.org/10.1038/s44383-025-00002-8</a>","chicago":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” <i>Npj Science of Plants</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s44383-025-00002-8\">https://doi.org/10.1038/s44383-025-00002-8</a>.","short":"A. Monzer, J. Friml, Npj Science of Plants 1 (2025) 2.","ieee":"A. Monzer and J. Friml, “Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.,” <i>npj Science of Plants</i>, vol. 1, no. 1. Springer Nature, p. 2, 2025.","ista":"Monzer A, Friml J. 2025. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 1(1), 2.","mla":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” <i>Npj Science of Plants</i>, vol. 1, no. 1, Springer Nature, 2025, p. 2, doi:<a href=\"https://doi.org/10.1038/s44383-025-00002-8\">10.1038/s44383-025-00002-8</a>.","ama":"Monzer A, Friml J. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. <i>npj Science of Plants</i>. 2025;1(1):2. doi:<a href=\"https://doi.org/10.1038/s44383-025-00002-8\">10.1038/s44383-025-00002-8</a>"},"page":"2","OA_place":"publisher","author":[{"last_name":"Monzer","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","full_name":"Monzer, Aline","first_name":"Aline"},{"first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"file_date_updated":"2026-02-10T09:35:43Z","doi":"10.1038/s44383-025-00002-8","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"article_type":"original","date_published":"2025-07-01T00:00:00Z","publication_identifier":{"eissn":["3005-1401"]},"external_id":{"pmid":["40630787"]},"publication_status":"published","oa":1,"project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"},{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"volume":1,"fulldoi":"https://doi.org/10.1038/s44383-025-00002-8","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21136","corr_author":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"day":"01","date_created":"2026-02-03T13:03:53Z","ddc":["580"],"publication":"npj Science of Plants","month":"07","file":[{"creator":"dernst","relation":"main_file","success":1,"file_size":974106,"date_created":"2026-02-10T09:35:43Z","content_type":"application/pdf","checksum":"6c190faacf0e3bef98311dc8a12132d4","file_id":"21208","access_level":"open_access","date_updated":"2026-02-10T09:35:43Z","file_name":"2025_NPJSciencePlants_Monzer.pdf"}],"date_updated":"2026-02-10T09:39:20Z","has_accepted_license":"1","intvolume":"         1","title":"Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.","abstract":[{"lang":"eng","text":"The plant hormone auxin regulates growth and development through at least two distinct signaling pathways. The nuclear pathway, involving TIR1/AFB receptors, mediates transcription; whereas the cell surface ABP1-TMK1 auxin perception triggers global ultrafast phosphorylation response. Here, we revisit the rich history of the disputed ABP1 auxin receptor, highlighting recent findings of the involvement of TMKs and other molecular components and focusing on their role in auxin canalization-mediated development."}],"year":"2025","article_processing_charge":"Yes (in subscription journal)","OA_type":"hybrid","type":"journal_article","pmid":1,"acknowledgement":"We gratefully acknowledge the funding by the Austrian Science Fund (FWF; I 6123-B and P 37051-B) and the European Research Council (ERC; 101142681 CYNIPS).We would like to thank Lukas Fiedler for his significant input and thoughtful revision of this manuscript.","issue":"1"},{"month":"05","date_updated":"2026-02-17T13:28:38Z","page":"299-322","alternative_title":["Foundations and Frontiers in Enzymology"],"author":[{"last_name":"Qi","full_name":"Qi, Linlin","first_name":"Linlin"},{"first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"publication":"Cryptic Enzymes and Moonlighting Proteins","quality_controlled":"1","citation":{"ieee":"L. Qi and J. Friml, “Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling,” in <i>Cryptic Enzymes and Moonlighting Proteins</i>, H. Irving, C. Gehring, and A. Wong, Eds. Elsevier, 2025, pp. 299–322.","short":"L. Qi, J. Friml, in:, H. Irving, C. Gehring, A. Wong (Eds.), Cryptic Enzymes and Moonlighting Proteins, Elsevier, 2025, pp. 299–322.","chicago":"Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” In <i>Cryptic Enzymes and Moonlighting Proteins</i>, edited by Helen Irving, Chris Gehring, and Aloysius Wong, 299–322. Elsevier, 2025. <a href=\"https://doi.org/10.1016/b978-0-443-15719-6.00015-5\">https://doi.org/10.1016/b978-0-443-15719-6.00015-5</a>.","apa":"Qi, L., &#38; Friml, J. (2025). Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In H. Irving, C. Gehring, &#38; A. Wong (Eds.), <i>Cryptic Enzymes and Moonlighting Proteins</i> (pp. 299–322). Elsevier. <a href=\"https://doi.org/10.1016/b978-0-443-15719-6.00015-5\">https://doi.org/10.1016/b978-0-443-15719-6.00015-5</a>","ama":"Qi L, Friml J. Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Irving H, Gehring C, Wong A, eds. <i>Cryptic Enzymes and Moonlighting Proteins</i>. Elsevier; 2025:299-322. doi:<a href=\"https://doi.org/10.1016/b978-0-443-15719-6.00015-5\">10.1016/b978-0-443-15719-6.00015-5</a>","mla":"Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” <i>Cryptic Enzymes and Moonlighting Proteins</i>, edited by Helen Irving et al., Elsevier, 2025, pp. 299–322, doi:<a href=\"https://doi.org/10.1016/b978-0-443-15719-6.00015-5\">10.1016/b978-0-443-15719-6.00015-5</a>.","ista":"Qi L, Friml J. 2025.Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Cryptic Enzymes and Moonlighting Proteins. Foundations and Frontiers in Enzymology, , 299–322."},"publisher":"Elsevier","status":"public","date_published":"2025-05-02T00:00:00Z","year":"2025","publication_identifier":{"isbn":["9780443157196"]},"abstract":[{"text":"As an important plant hormone to regulate growth and development, auxin has been investigated for more than a century. It had been clearly demonstrated and well-accepted that the intracellular auxin receptors, TIR1/AFBs, are F-box proteins mediating transcriptional auxin signaling by their E3 ubiquitin ligase activity, which targets and sends for degradation the Aux/IAA transcriptional repressors. The recent discovery of adenylate cyclase (AC) and guanylate cyclase (GC) activities for TIR1/AFBs open entirely new perspectives on how auxin signaling can operate. This chapter traces back the history of how canonical transcriptional auxin signaling was established and introduces the discovery of the TIR1/AFBs-mediated nontranscriptional signaling branch. Finally, the current understanding and open questions of how TIR1/AFBs’ AC and GC activities contribute to the transcriptional and nontranscriptional auxin signaling are discussed, highlighting the possibility that cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) act as second messengers in auxin signal transduction.","lang":"eng"}],"editor":[{"last_name":"Irving","full_name":"Irving, Helen","first_name":"Helen"},{"last_name":"Gehring","first_name":"Chris","full_name":"Gehring, Chris"},{"first_name":"Aloysius","full_name":"Wong, Aloysius","last_name":"Wong"}],"doi":"10.1016/b978-0-443-15719-6.00015-5","department":[{"_id":"JiFr"}],"title":"Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"book_chapter","fulldoi":"https://doi.org/10.1016/b978-0-443-15719-6.00015-5","OA_type":"closed access","article_processing_charge":"No","publication_status":"published","date_created":"2026-02-16T15:53:52Z","day":"02","scopus_import":"1","oa_version":"None","language":[{"iso":"eng"}],"_id":"21255"},{"day":"05","date_created":"2025-03-19T09:44:19Z","ec_funded":1,"_id":"19420","language":[{"iso":"eng"}],"oa_version":"None","corr_author":"1","scopus_import":"1","fulldoi":"https://doi.org/10.1093/pcp/pcaf008","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_status":"published","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"}],"article_type":"original","external_id":{"isi":["001436802900001"],"pmid":["39829340"]},"publication_identifier":{"issn":["0032-0781"],"eissn":["1471-9053"]},"date_published":"2025-03-05T00:00:00Z","department":[{"_id":"JiFr"}],"doi":"10.1093/pcp/pcaf008","citation":{"apa":"Tang, H., Chen, L., &#38; Friml, J. (2025). Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcaf008\">https://doi.org/10.1093/pcp/pcaf008</a>","chicago":"Tang, Han, L Chen, and Jiří Friml. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/pcp/pcaf008\">https://doi.org/10.1093/pcp/pcaf008</a>.","short":"H. Tang, L. Chen, J. Friml, Plant and Cell Physiology (2025).","ieee":"H. Tang, L. Chen, and J. Friml, “Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.,” <i>Plant and Cell Physiology</i>. Oxford University Press, 2025.","ista":"Tang H, Chen L, Friml J. 2025. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology., pcaf008.","mla":"Tang, Han, et al. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” <i>Plant and Cell Physiology</i>, pcaf008, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/pcp/pcaf008\">10.1093/pcp/pcaf008</a>.","ama":"Tang H, Chen L, Friml J. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. <i>Plant and Cell Physiology</i>. 2025. doi:<a href=\"https://doi.org/10.1093/pcp/pcaf008\">10.1093/pcp/pcaf008</a>"},"isi":1,"quality_controlled":"1","author":[{"orcid":"0000-0001-6152-6637","last_name":"Tang","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","full_name":"Tang, Han","first_name":"Han"},{"last_name":"Chen","full_name":"Chen, L","first_name":"L"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří"}],"status":"public","publisher":"Oxford University Press","acknowledgement":"The authors sincerely thank Dr Barbara Kloeckener Gruissem’s time and efforts in critical reading and constructive advice on the manuscript. The authors gratefully acknowledge Dr. Eva Sundberg for generously providing transgenic plants to support this study.\r\nThis work was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.) and the Taiwan National Science and Technology Council (NSTC 112-2311-B-005-008 to H.T. and L.-H.C.).","article_number":"pcaf008","type":"journal_article","pmid":1,"article_processing_charge":"No","OA_type":"closed access","abstract":[{"lang":"eng","text":"Auxin and its PIN-FORMED (PIN) exporters are essential for tissue repair and regeneration in flowering plants. To gain insight into the evolution of this mechanism, we investigated their roles in leaves excised from Physcomitrium patens, a bryophyte known for its remarkable cell reprogramming capacity. We used various approaches to manipulate auxin levels, including exogenous application, pharmacological manipulations, and auxin biosynthesis mutants. We observed no significant effect on the rate of cell reprogramming. Rather, our analysis of auxin dynamics revealed a decrease in auxin levels upon excision, which was followed by a local increase before the reprogramming process began. Mutant analysis revealed that PpPINs are required for effective cell reprogramming, and endogenously expressed PpPINA-GFP accumulates polarly at sites that will develop into future filamentous stem cells. In addition, hyperpolarized PpPINA variants carrying mutated phosphorylation sites showed a marked delay in reprogramming, whereas endogenous or nonpolar versions do not have this effect. These results underscore that both the levels and the polarity of PpPINA are important for efficient cell reprogramming. Overall, these findings highlight the pivotal role of PIN polarity in plant regeneration. Furthermore, they suggest that understanding polarity mechanisms could have broader implications for improving regenerative processes across various plant species."}],"year":"2025","title":"Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.","publication":"Plant and Cell Physiology","date_updated":"2025-09-30T11:05:55Z","month":"03"},{"publication":"Nature Plants","date_updated":"2025-11-12T07:52:06Z","month":"03","file":[{"date_created":"2025-11-12T07:50:45Z","file_size":2714177,"content_type":"application/pdf","checksum":"caeaf1a8bc3e1435e8c995d1d9df5390","creator":"dernst","success":1,"relation":"main_file","file_name":"2025_NaturePlants_Jia_submitted.pdf","date_updated":"2025-11-12T07:50:45Z","access_level":"open_access","file_id":"20634"}],"abstract":[{"lang":"eng","text":"Nitrogen (N) is an essential macronutrient for plant development and, ultimately, yield. Identifying the genetic components and mechanisms underlying N use efficiency in maize (Zea mays L.) is thus of great importance. Nitrate (NO3−) is the preferred inorganic N source in maize. Here we performed a genome-wide association study of shoot NO3− accumulation in maize seedlings grown under low-NO3− conditions, identifying the ferredoxin family gene ZmFd4 as a major contributor to this trait. ZmFd4 interacts and co-localizes with nitrite reductases (ZmNiRs) in chloroplasts to promote their enzymatic activity. Furthermore, ZmFd4 forms a high-affinity heterodimer with its closest paralogue, ZmFd9, in a NO3−-sensitive manner. Although ZmFd4 exerts similar biochemical functions as ZmFd9, ZmFd4 and ZmFd9 interaction limits their ability to associate with ZmNiRs and stimulate their activity. Knockout lines for ZmFd4 with decreased NO3− contents exhibit more efficient NO3− assimilation, and field experiments show consistently improved N utilization and grain yield under N-deficient conditions. Our work thus provides molecular and mechanistic insights into the natural variation in N utilization, instrumental for genetic improvement of yield in maize and, potentially, in other crops."}],"year":"2025","title":"Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize","intvolume":"        11","has_accepted_license":"1","type":"journal_article","pmid":1,"article_processing_charge":"No","OA_type":"green","acknowledgement":"We thank X. Yang for providing published inbred lines and helping with data analysis; and S. Huang, C. Jiang, G. Bi, C. Liu and S. Zhang for helpful discussions. The transgenic maize lines were generated by the Center for Crop Functional Genomics and Molecular Breeding of China Agricultural University. This work was supported by grants from the National Key Research and Development Program of China (2021YFF1000500 to J.Z.), the National Natural Science Foundation of China (32170265 and 32441022 to J.Z.), the Chinese Universities Scientific Fund (2024TC084 to J.Z.), the Pinduoduo-China Agricultural University Research Fund (PC2024B01005 to J.Z.), the Hainan Provincial Natural Science Foundation of China (323CXTD379 to J.Z.), and the Central Guidance on Local Science and Technology Development Fund of Shanxi Province (YDZJSX2024D040 to C.T. and J.Z.).","article_number":"5207","citation":{"short":"G. Jia, G. Chen, Z. Zhang, C. Tian, Y. Wang, J. Luo, K. Zhang, X. Zhao, X. Zhao, Z. Li, L. Sun, W. Yang, Y. Guo, J. Friml, Z. Gong, J. Zhang, Nature Plants 11 (2025).","ieee":"G. Jia <i>et al.</i>, “Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize,” <i>Nature Plants</i>, vol. 11. Springer Nature, 2025.","apa":"Jia, G., Chen, G., Zhang, Z., Tian, C., Wang, Y., Luo, J., … Zhang, J. (2025). Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-025-01934-w\">https://doi.org/10.1038/s41477-025-01934-w</a>","chicago":"Jia, G, G Chen, Z Zhang, C Tian, Y Wang, J Luo, K Zhang, et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” <i>Nature Plants</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41477-025-01934-w\">https://doi.org/10.1038/s41477-025-01934-w</a>.","ama":"Jia G, Chen G, Zhang Z, et al. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. <i>Nature Plants</i>. 2025;11. doi:<a href=\"https://doi.org/10.1038/s41477-025-01934-w\">10.1038/s41477-025-01934-w</a>","ista":"Jia G, Chen G, Zhang Z, Tian C, Wang Y, Luo J, Zhang K, Zhao X, Zhao X, Li Z, Sun L, Yang W, Guo Y, Friml J, Gong Z, Zhang J. 2025. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. 11, 5207.","mla":"Jia, G., et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” <i>Nature Plants</i>, vol. 11, 5207, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41477-025-01934-w\">10.1038/s41477-025-01934-w</a>."},"isi":1,"quality_controlled":"1","author":[{"last_name":"Jia","full_name":"Jia, G","first_name":"G"},{"last_name":"Chen","full_name":"Chen, G","first_name":"G"},{"first_name":"Z","full_name":"Zhang, Z","last_name":"Zhang"},{"last_name":"Tian","full_name":"Tian, C","first_name":"C"},{"last_name":"Wang","full_name":"Wang, Y","first_name":"Y"},{"last_name":"Luo","full_name":"Luo, J","first_name":"J"},{"first_name":"K","full_name":"Zhang, K","last_name":"Zhang"},{"full_name":"Zhao, X","first_name":"X","last_name":"Zhao"},{"first_name":"X","full_name":"Zhao, X","last_name":"Zhao"},{"full_name":"Li, Z","first_name":"Z","last_name":"Li"},{"last_name":"Sun","first_name":"L","full_name":"Sun, L"},{"full_name":"Yang, W","first_name":"W","last_name":"Yang"},{"full_name":"Guo, Y","first_name":"Y","last_name":"Guo"},{"first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"},{"full_name":"Gong, Z","first_name":"Z","last_name":"Gong"},{"last_name":"Zhang","full_name":"Zhang, J","first_name":"J"}],"OA_place":"repository","status":"public","publisher":"Springer Nature","article_type":"original","publication_identifier":{"issn":["2055-0278"]},"external_id":{"pmid":["40044942"],"isi":["001437953800001"]},"date_published":"2025-03-05T00:00:00Z","department":[{"_id":"JiFr"}],"file_date_updated":"2025-11-12T07:50:45Z","doi":"10.1038/s41477-025-01934-w","fulldoi":"https://doi.org/10.1038/s41477-025-01934-w","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","oa":1,"volume":11,"date_created":"2025-03-19T09:44:55Z","day":"05","ddc":["580"],"_id":"19422","language":[{"iso":"eng"}],"oa_version":"Submitted Version","scopus_import":"1"},{"fulldoi":"https://doi.org/10.1111/nph.70019","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa":1,"publication_status":"published","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"volume":246,"day":"01","date_created":"2025-03-19T09:45:11Z","ddc":["580"],"_id":"19423","oa_version":"Published Version","language":[{"iso":"eng"}],"scopus_import":"1","citation":{"short":"K. Kurtović, S. Vosolsobě, D. Nedvěd, K. Müller, P. Dobrev, V. Schmidt, P. Piszczek, A. Kuhn, A. Smoljan, T. Fisher, D. Weijers, J. Friml, J. Bowman, J. Petrášek, New Phytologist 246 (2025) 1066–1083.","ieee":"K. Kurtović <i>et al.</i>, “The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii,” <i>New Phytologist</i>, vol. 246, no. 3. Wiley, pp. 1066–1083, 2025.","apa":"Kurtović, K., Vosolsobě, S., Nedvěd, D., Müller, K., Dobrev, P., Schmidt, V., … Petrášek, J. (2025). The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.70019\">https://doi.org/10.1111/nph.70019</a>","chicago":"Kurtović, K, S Vosolsobě, D Nedvěd, K Müller, PI Dobrev, V Schmidt, P Piszczek, et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” <i>New Phytologist</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/nph.70019\">https://doi.org/10.1111/nph.70019</a>.","ama":"Kurtović K, Vosolsobě S, Nedvěd D, et al. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. <i>New Phytologist</i>. 2025;246(3):1066-1083. doi:<a href=\"https://doi.org/10.1111/nph.70019\">10.1111/nph.70019</a>","ista":"Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev P, Schmidt V, Piszczek P, Kuhn A, Smoljan A, Fisher T, Weijers D, Friml J, Bowman J, Petrášek J. 2025. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 246(3), 1066–1083.","mla":"Kurtović, K., et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” <i>New Phytologist</i>, vol. 246, no. 3, Wiley, 2025, pp. 1066–83, doi:<a href=\"https://doi.org/10.1111/nph.70019\">10.1111/nph.70019</a>."},"isi":1,"quality_controlled":"1","page":"1066-1083","OA_place":"publisher","author":[{"first_name":"K","full_name":"Kurtović, K","last_name":"Kurtović"},{"last_name":"Vosolsobě","full_name":"Vosolsobě, S","first_name":"S"},{"full_name":"Nedvěd, D","first_name":"D","last_name":"Nedvěd"},{"first_name":"K","full_name":"Müller, K","last_name":"Müller"},{"full_name":"Dobrev, PI","first_name":"PI","last_name":"Dobrev"},{"first_name":"V","full_name":"Schmidt, V","last_name":"Schmidt"},{"first_name":"P","full_name":"Piszczek, P","last_name":"Piszczek"},{"last_name":"Kuhn","first_name":"A","full_name":"Kuhn, A"},{"full_name":"Smoljan, Adrijana","first_name":"Adrijana","last_name":"Smoljan","id":"cced8a85-223e-11ed-af04-b0596c55053b"},{"first_name":"TJ","full_name":"Fisher, TJ","last_name":"Fisher"},{"full_name":"Weijers, D","first_name":"D","last_name":"Weijers"},{"first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"first_name":"JL","full_name":"Bowman, JL","last_name":"Bowman"},{"full_name":"Petrášek, J","first_name":"J","last_name":"Petrášek"}],"status":"public","publisher":"Wiley","article_type":"original","publication_identifier":{"issn":["1469-8137"]},"external_id":{"pmid":["40047465"],"isi":["001438711600001"]},"date_published":"2025-05-01T00:00:00Z","department":[{"_id":"JiFr"}],"file_date_updated":"2025-04-16T08:03:36Z","doi":"10.1111/nph.70019","type":"journal_article","pmid":1,"article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","acknowledgement":"This work was supported by funding from the Czech Science Foundation project no. 20-13587S to JP and SV, Charles University Grant Agency projects no. 289523 to KK and no. 393422 to VS, a DOC fellowship of the Austrian Academy of Sciences to AS, and the Austrian Science Fund (FWF): I 6123-B to JF. The authors acknowledge the Imaging Facility of the Institute of Experimental Botany AS CR supported by the MEYS CR (LM2023050 Czech-BioImaging), the Czech Academy of Sciences and IEB AS CR, and Viničná Microscopy Core Facility cofinanced by the Czech-BioImaging large RI project LM2023050. Computational resources were provided by the e-INFRA CZ project (ID:90254), supported by the MEYS CR. The authors would like to thank Ilse Foissner and Margit Höftberger for discussing details of immunostaining protocol, Katarzyna Retzer and Jan Martinek for help with western blots, Anna Kampová for help with phosphoproteome sampling, Anja Holzhausen and MadLAnd for providing Chara braunii strain S276, and Roman Skokan for valuable discussion. Open access publishing facilitated by Univerzita Karlova, as part of the Wiley - CzechELib agreement.","issue":"3","publication":"New Phytologist","date_updated":"2025-09-30T11:11:18Z","file":[{"success":1,"relation":"main_file","creator":"dernst","content_type":"application/pdf","checksum":"861c9bf47e7a7766ed03e6d85bd4f6dc","date_created":"2025-04-16T08:03:36Z","file_size":12841729,"access_level":"open_access","file_id":"19571","file_name":"2025_NewPhytologist_Kurtovic.pdf","date_updated":"2025-04-16T08:03:36Z"}],"month":"05","abstract":[{"text":"Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii.\r\nHere, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.\r\nThe thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase.\r\nThese findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes.","lang":"eng"}],"year":"2025","intvolume":"       246","title":"The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii","has_accepted_license":"1"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1038/s41477-025-01975-1","volume":11,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publication_status":"published","oa":1,"ddc":["580"],"day":"11","date_created":"2025-04-20T22:01:28Z","oa_version":"Published Version","language":[{"iso":"eng"}],"scopus_import":"1","_id":"19601","author":[{"last_name":"De Roij","full_name":"De Roij, Martijn","first_name":"Martijn"},{"last_name":"Hernández García","first_name":"Jorge","full_name":"Hernández García, Jorge"},{"last_name":"Das","id":"b08969a4-f2a5-11ed-b6c4-ff0f10b7d0be","full_name":"Das, Shubhajit","first_name":"Shubhajit"},{"last_name":"Borst","full_name":"Borst, Jan Willem","first_name":"Jan Willem"},{"last_name":"Weijers","first_name":"Dolf","full_name":"Weijers, Dolf"}],"page":"717-724","OA_place":"publisher","citation":{"ama":"De Roij M, Hernández García J, Das S, Borst JW, Weijers D. ARF degradation defines a deeply conserved step in auxin response. <i>Nature Plants</i>. 2025;11:717-724. doi:<a href=\"https://doi.org/10.1038/s41477-025-01975-1\">10.1038/s41477-025-01975-1</a>","mla":"De Roij, Martijn, et al. “ARF Degradation Defines a Deeply Conserved Step in Auxin Response.” <i>Nature Plants</i>, vol. 11, Springer Nature, 2025, pp. 717–24, doi:<a href=\"https://doi.org/10.1038/s41477-025-01975-1\">10.1038/s41477-025-01975-1</a>.","ista":"De Roij M, Hernández García J, Das S, Borst JW, Weijers D. 2025. ARF degradation defines a deeply conserved step in auxin response. Nature Plants. 11, 717–724.","ieee":"M. De Roij, J. Hernández García, S. Das, J. W. Borst, and D. Weijers, “ARF degradation defines a deeply conserved step in auxin response,” <i>Nature Plants</i>, vol. 11. Springer Nature, pp. 717–724, 2025.","short":"M. De Roij, J. Hernández García, S. Das, J.W. Borst, D. Weijers, Nature Plants 11 (2025) 717–724.","chicago":"De Roij, Martijn, Jorge Hernández García, Shubhajit Das, Jan Willem Borst, and Dolf Weijers. “ARF Degradation Defines a Deeply Conserved Step in Auxin Response.” <i>Nature Plants</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41477-025-01975-1\">https://doi.org/10.1038/s41477-025-01975-1</a>.","apa":"De Roij, M., Hernández García, J., Das, S., Borst, J. W., &#38; Weijers, D. (2025). ARF degradation defines a deeply conserved step in auxin response. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-025-01975-1\">https://doi.org/10.1038/s41477-025-01975-1</a>"},"quality_controlled":"1","publisher":"Springer Nature","status":"public","publication_identifier":{"eissn":["2055-0278"]},"external_id":{"pmid":["40216983"]},"date_published":"2025-04-11T00:00:00Z","article_type":"letter_note","department":[{"_id":"JiFr"}],"file_date_updated":"2025-12-30T07:28:09Z","doi":"10.1038/s41477-025-01975-1","pmid":1,"type":"journal_article","OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)","acknowledgement":"We thank S. Woudenberg, S. Valk and J. Rienstra for help and advice, A. Kuhn for comments on the paper and M. Prigge and M. Estelle for helpful discussions. This work was supported by a grant from Netherlands Organization for Scientific Research (NWO; OCENW.M20.031 to J.W.B.), a Marie Skłodowska-Curie Individual Fellowship (H2020-MSCA-IF-2020 contract number to J.H.G.) and a research grant from the Human Frontiers Research Program (HFSP; grant RGP0015/2022 to D.W.).","date_updated":"2025-12-30T07:28:49Z","file":[{"file_id":"20882","access_level":"open_access","date_updated":"2025-12-30T07:28:09Z","file_name":"2025_NaturePlants_deRoij.pdf","creator":"dernst","relation":"main_file","success":1,"file_size":7062474,"date_created":"2025-12-30T07:28:09Z","content_type":"application/pdf","checksum":"8225c1899bb2f39f9a1707cc0697a052"}],"month":"04","publication":"Nature Plants","year":"2025","abstract":[{"text":"In land plants, the signalling molecule auxin profoundly controls growth and development, chiefly through a transcriptional response system. The auxin response is mediated by modulating the activity of DNA-binding auxin response factor (ARF) proteins. The concentrations and stoichiometry of the competing A- and B-class ARFs define cells’ capacity for auxin response. In the minimal auxin response system of the liverwort Marchantia polymorpha, both A- and B-ARFs are unstable, but the underlying mechanisms, developmental relevance and evolutionary history of this instability are unknown. Here we identify a minimal motif that is necessary for MpARF2 (B-class) degradation and show that it is critical for development and the auxin response. Through comparative analysis and motif swaps among all ARF classes in extant algae and land plants, we infer that the emergence of ARF instability probably occurred in the ancestor of the A- and B-ARF clades and, therefore, preceded or coincided with the origin of the auxin response system.","lang":"eng"}],"title":"ARF degradation defines a deeply conserved step in auxin response","intvolume":"        11","has_accepted_license":"1"},{"publication_status":"published","oa":1,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742985"},{"grant_number":"I03630","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425"}],"volume":122,"fulldoi":"https://doi.org/10.1073/pnas.2427315122","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"19728","scopus_import":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"corr_author":"1","date_created":"2025-05-25T22:16:43Z","day":"20","ddc":["580"],"ec_funded":1,"status":"public","publisher":"National Academy of Sciences","quality_controlled":"1","isi":1,"citation":{"mla":"Zhang, Yuzhou, et al. “Foraging for Water by MIZ1-Mediated Antagonism between Root Gravitropism and Hydrotropism.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 20, e2427315122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2427315122\">10.1073/pnas.2427315122</a>.","ista":"Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. 2025. Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. Proceedings of the National Academy of Sciences. 122(20), e2427315122.","ama":"Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(20). doi:<a href=\"https://doi.org/10.1073/pnas.2427315122\">10.1073/pnas.2427315122</a>","chicago":"Zhang, Yuzhou, Zhulatai Bao, Adrijana Smoljan, Yifan Liu, Huihui Wang, and Jiří Friml. “Foraging for Water by MIZ1-Mediated Antagonism between Root Gravitropism and Hydrotropism.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2427315122\">https://doi.org/10.1073/pnas.2427315122</a>.","apa":"Zhang, Y., Bao, Z., Smoljan, A., Liu, Y., Wang, H., &#38; Friml, J. (2025). Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2427315122\">https://doi.org/10.1073/pnas.2427315122</a>","ieee":"Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, and J. Friml, “Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 20. National Academy of Sciences, 2025.","short":"Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, J. Friml, Proceedings of the National Academy of Sciences 122 (2025)."},"author":[{"first_name":"Yuzhou","full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","last_name":"Zhang","orcid":"0000-0003-2627-6956"},{"full_name":"Bao, Zhulatai","first_name":"Zhulatai","last_name":"Bao"},{"first_name":"Adrijana","full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","last_name":"Smoljan"},{"last_name":"Liu","full_name":"Liu, Yifan","first_name":"Yifan"},{"last_name":"Wang","full_name":"Wang, Huihui","first_name":"Huihui"},{"full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"OA_place":"publisher","file_date_updated":"2025-05-28T08:04:50Z","doi":"10.1073/pnas.2427315122","department":[{"_id":"JiFr"}],"article_type":"original","date_published":"2025-05-20T00:00:00Z","external_id":{"isi":["001496347500001"],"pmid":["40372432"]},"publication_identifier":{"eissn":["1091-6490"]},"article_processing_charge":"Yes (in subscription journal)","OA_type":"hybrid","type":"journal_article","pmid":1,"article_number":"e2427315122","acknowledgement":"This work was supported by the European Union’s Horizon 2020 research and innovation Programme (European Research Council grant agreement number 742985), Austrian Science Fund (FWF, grant number I 3630-B25), (Institute of Science and Technology Austria) Fellow program, the Qin Chuangyuan High-level Innovation and Entrepreneurship Talent Program (QCYRCXM-2022-237), the Fundamental Research Funds for Northwest A&F University and partly supported by the open funds of the State Key Laboratory of Plant Environmental Resilience (SKLPERKF2416). We also thank the Teaching and Research Core Facility at the College of Life Sciences, Northwest A&F University, particularly Dr. Ningjuan Fan for technical assistance.","issue":"20","publication":"Proceedings of the National Academy of Sciences","month":"05","file":[{"content_type":"application/pdf","checksum":"f70ff35054561b27a463ba279d1795dc","file_size":8266672,"date_created":"2025-05-28T08:04:50Z","relation":"main_file","success":1,"creator":"dernst","date_updated":"2025-05-28T08:04:50Z","file_name":"2025_PNAS_Zhang.pdf","file_id":"19750","access_level":"open_access"}],"date_updated":"2026-05-20T08:34:21Z","has_accepted_license":"1","title":"Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism","intvolume":"       122","related_material":{"link":[{"url":"https://ista.ac.at/en/news/how-roots-forage-for-water/","description":"News on ISTA website","relation":"press_release"}]},"APC_amount":"5937,40 EUR","abstract":[{"text":"Root system integrates multiple environmental cues, chiefly gravity and soil humidity, to anchor plants in soil and forage for water. While the mechanism of auxin-mediated root gravitropism is comparably well-understood, the root’s capability to grow toward moist soil for water uptake and drought avoidance, termed root hydrotropism, remains largely mysterious. Here, we provide key insights into the mechanism of hydrotropic growth and assign a role to the master regulator of hydrotropism, MIZU-KUSSEI 1 (MIZ1). We show that efficient hydrotropism requires the attenuation of antagonistically acting gravitropism, which is inhibited under drought conditions. Drought stress interferes with subcellular trafficking and the lateral mobility of PIN auxin transporters, which are polarly localized at the root cell plasma membranes. This leads to defects in PIN2 polarity and gravity-induced polarization of PIN3, ultimately inhibiting gravity-induced auxin redistribution and root bending. The miz1 mutant is defective in all these regulations, and in support of MIZ1’s action on PINs, pin mutations rescue the hydrotropic defects in the miz1 mutant. These observations identify a mechanism for how drought via MIZ1 attenuates gravitropism to promote root hydrotropism for efficient water foraging under drought conditions.","lang":"eng"}],"year":"2025"},{"publisher":"Springer Nature","status":"public","author":[{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"id":"98605edc-6ce7-11ee-95f3-cc16b866efcd","last_name":"Pei","first_name":"Yuanrong","full_name":"Pei, Yuanrong"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří"}],"quality_controlled":"1","isi":1,"citation":{"ieee":"S. Vanneste, Y. Pei, and J. Friml, “Mechanisms of auxin action in plant growth and development,” <i>Nature Reviews Molecular Cell Biology</i>. Springer Nature, 2025.","short":"S. Vanneste, Y. Pei, J. Friml, Nature Reviews Molecular Cell Biology (2025).","chicago":"Vanneste, Steffen, Yuanrong Pei, and Jiří Friml. “Mechanisms of Auxin Action in Plant Growth and Development.” <i>Nature Reviews Molecular Cell Biology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41580-025-00851-2\">https://doi.org/10.1038/s41580-025-00851-2</a>.","apa":"Vanneste, S., Pei, Y., &#38; Friml, J. (2025). Mechanisms of auxin action in plant growth and development. <i>Nature Reviews Molecular Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41580-025-00851-2\">https://doi.org/10.1038/s41580-025-00851-2</a>","ama":"Vanneste S, Pei Y, Friml J. Mechanisms of auxin action in plant growth and development. <i>Nature Reviews Molecular Cell Biology</i>. 2025. doi:<a href=\"https://doi.org/10.1038/s41580-025-00851-2\">10.1038/s41580-025-00851-2</a>","mla":"Vanneste, Steffen, et al. “Mechanisms of Auxin Action in Plant Growth and Development.” <i>Nature Reviews Molecular Cell Biology</i>, e113018, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41580-025-00851-2\">10.1038/s41580-025-00851-2</a>.","ista":"Vanneste S, Pei Y, Friml J. 2025. Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology., e113018."},"doi":"10.1038/s41580-025-00851-2","department":[{"_id":"JiFr"}],"date_published":"2025-05-19T00:00:00Z","external_id":{"isi":["001490500500001"],"pmid":["40389696"]},"publication_identifier":{"eissn":["1471-0080"],"issn":["1471-0072"]},"article_type":"review","publication_status":"published","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","fulldoi":"https://doi.org/10.1038/s41580-025-00851-2","scopus_import":"1","corr_author":"1","language":[{"iso":"eng"}],"oa_version":"None","_id":"19736","day":"19","date_created":"2025-05-25T22:16:57Z","month":"05","date_updated":"2025-09-30T12:41:30Z","publication":"Nature Reviews Molecular Cell Biology","title":"Mechanisms of auxin action in plant growth and development","year":"2025","abstract":[{"lang":"eng","text":"The phytohormone auxin is a major signal coordinating growth and development in plants. The variety of its effects arises from its ability to form local auxin maxima and gradients within tissues, generated through directional cell-to-cell transport and elaborate metabolic control. These auxin distribution patterns instruct cells in a context-dependent manner to undergo predefined developmental transitions. In this Review, we discuss advances in auxin action at the level of homeostasis and signalling. We highlight key insights into the structural basis of PIN-mediated intercellular auxin transport and explore two novel non-transcriptional auxin signalling mechanisms: one involving intracellular Ca2+ transients and another involving cell-surface auxin perception that mediates global, ultrafast phosphorylation. Furthermore, we examine emerging evidence indicating the involvement of cyclic adenosine monophosphate as a second messenger in the transcriptional auxin response. Together, these recent developments in auxin research have profoundly deepened our understanding of the complex and diverse activities of auxin in plant growth and development."}],"OA_type":"closed access","article_processing_charge":"No","pmid":1,"type":"journal_article","article_number":"e113018"},{"publisher":"Oxford University Press","status":"public","page":"2700-2714","author":[{"first_name":"Claudio","full_name":"Osorio-Navarro, Claudio","last_name":"Osorio-Navarro"},{"full_name":"Neira-Valenzuela, Gabriel","first_name":"Gabriel","last_name":"Neira-Valenzuela"},{"last_name":"Sierra","first_name":"Paula","full_name":"Sierra, Paula"},{"first_name":"Maciek","full_name":"Adamowski, Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","last_name":"Adamowski","orcid":"0000-0001-6463-5257"},{"last_name":"Toledo","first_name":"Jorge","full_name":"Toledo, Jorge"},{"last_name":"Norambuena","first_name":"Lorena","full_name":"Norambuena, Lorena"}],"quality_controlled":"1","citation":{"mla":"Osorio-Navarro, Claudio, et al. “The Configuration of the Vacuole Is Driven by Clathrin-Mediated Trafficking in Root Cells of Arabidopsis Thaliana.” <i>Journal of Experimental Botany</i>, vol. 76, no. 10, Oxford University Press, 2025, pp. 2700–14, doi:<a href=\"https://doi.org/10.1093/jxb/eraf084\">10.1093/jxb/eraf084</a>.","ista":"Osorio-Navarro C, Neira-Valenzuela G, Sierra P, Adamowski M, Toledo J, Norambuena L. 2025. The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. Journal of Experimental Botany. 76(10), 2700–2714.","ama":"Osorio-Navarro C, Neira-Valenzuela G, Sierra P, Adamowski M, Toledo J, Norambuena L. The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. <i>Journal of Experimental Botany</i>. 2025;76(10):2700-2714. doi:<a href=\"https://doi.org/10.1093/jxb/eraf084\">10.1093/jxb/eraf084</a>","chicago":"Osorio-Navarro, Claudio, Gabriel Neira-Valenzuela, Paula Sierra, Maciek Adamowski, Jorge Toledo, and Lorena Norambuena. “The Configuration of the Vacuole Is Driven by Clathrin-Mediated Trafficking in Root Cells of Arabidopsis Thaliana.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/jxb/eraf084\">https://doi.org/10.1093/jxb/eraf084</a>.","apa":"Osorio-Navarro, C., Neira-Valenzuela, G., Sierra, P., Adamowski, M., Toledo, J., &#38; Norambuena, L. (2025). The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/eraf084\">https://doi.org/10.1093/jxb/eraf084</a>","ieee":"C. Osorio-Navarro, G. Neira-Valenzuela, P. Sierra, M. Adamowski, J. Toledo, and L. Norambuena, “The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana,” <i>Journal of Experimental Botany</i>, vol. 76, no. 10. Oxford University Press, pp. 2700–2714, 2025.","short":"C. Osorio-Navarro, G. Neira-Valenzuela, P. Sierra, M. Adamowski, J. Toledo, L. Norambuena, Journal of Experimental Botany 76 (2025) 2700–2714."},"isi":1,"doi":"10.1093/jxb/eraf084","department":[{"_id":"JiFr"}],"date_published":"2025-07-02T00:00:00Z","publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"external_id":{"pmid":["40056424"],"isi":["001482869200001"]},"article_type":"original","volume":76,"publication_status":"published","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","fulldoi":"https://doi.org/10.1093/jxb/eraf084","scopus_import":"1","language":[{"iso":"eng"}],"oa_version":"None","_id":"20031","day":"02","date_created":"2025-07-20T22:02:01Z","month":"07","date_updated":"2025-09-30T14:04:16Z","publication":"Journal of Experimental Botany","title":"The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana","intvolume":"        76","year":"2025","abstract":[{"text":"The central vacuole is a multifunctional organelle with the most significant occupancy in a differentiated plant cell. Plants depend on the function of the vacuole for critical development, growth, and environmental responses. As the cell expands, the vacuole changes shape and size, increasing its membrane and luminal content. The set of these events is called the vacuolar configuration process, which has not been well described. Our research highlights the impact of plasma membrane internalization on vacuole morphology during the vacuolar configuration process. We observed a direct correlation between differential endocytosis rates and the enrichment of vacuolar membranous structures. Chemical and genetic interference with clathrin-mediated endocytosis (CME) revealed that it is required for the vacuolar configuration of growing root cells. The contribution of CME to the vacuole configuration process co-occurs with the induction of post-trans-Golgi network (TGN)/early endosome (EE) trafficking with the participation of the Rab GTPases ARA6 and ARA7. Our results show that the CME plays an active role during vacuole configuration, most probably carrying the material that allows the establishment of the vacuole in elongating cells. Since membrane trafficking through the EE/TGN is required to reach the vacuole, additional players must be defined.","lang":"eng"}],"OA_type":"closed access","article_processing_charge":"No","pmid":1,"type":"journal_article","issue":"10","acknowledgement":"This research was supported by FONDECYT grants 1170950 and 1211311 and by ANID PhD fellowship 2020-21201663 to PhD student CO-N. The microscopes used in this work were funded by grants FONDEQUIP #EQM 140019 and #EQM12-0003 at the Advanced Microscopy Unit of the Biology Department, Faculty of Science, University of Chile.\r\nWe thank Jiri Friml for donating the XVE»AUXILIN-LIKE2 (AX2) line to support our research. We wish to acknowledge the active and helpful discussion of all the members of the LNM team and the Plant Molecular Biology Centre at Universidad de Chile."},{"publisher":"National Academy of Sciences","status":"public","OA_place":"publisher","author":[{"last_name":"Sheng","full_name":"Sheng, F","first_name":"F"},{"last_name":"Gao","full_name":"Gao, Y","first_name":"Y"},{"last_name":"Wang","full_name":"Wang, Y","first_name":"Y"},{"first_name":"Y","full_name":"Li, Y","last_name":"Li"},{"first_name":"JA","full_name":"Zhang, JA","last_name":"Zhang"},{"last_name":"Zhang","first_name":"Z","full_name":"Zhang, Z"},{"last_name":"Qin","first_name":"X","full_name":"Qin, X"},{"last_name":"Zhang","first_name":"S","full_name":"Zhang, S"},{"last_name":"Song","full_name":"Song, W","first_name":"W"},{"last_name":"Li","first_name":"J","full_name":"Li, J"},{"first_name":"Y","full_name":"Guo, Y","last_name":"Guo"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří"},{"last_name":"Gong","first_name":"Z","full_name":"Gong, Z"},{"last_name":"Zhang","full_name":"Zhang, Q","first_name":"Q"},{"first_name":"J","full_name":"Zhang, J","last_name":"Zhang"}],"page":"e2512274122","quality_controlled":"1","isi":1,"citation":{"short":"F. Sheng, Y. Gao, Y. Wang, Y. Li, J. Zhang, Z. Zhang, X. Qin, S. Zhang, W. Song, J. Li, Y. Guo, J. Friml, Z. Gong, Q. Zhang, J. Zhang, Proceedings of the National Academy of Sciences 122 (2025) e2512274122.","ieee":"F. Sheng <i>et al.</i>, “Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 39. National Academy of Sciences, p. e2512274122, 2025.","apa":"Sheng, F., Gao, Y., Wang, Y., Li, Y., Zhang, J., Zhang, Z., … Zhang, J. (2025). Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2512274122\">https://doi.org/10.1073/pnas.2512274122</a>","chicago":"Sheng, F, Y Gao, Y Wang, Y Li, JA Zhang, Z Zhang, X Qin, et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2512274122\">https://doi.org/10.1073/pnas.2512274122</a>.","ama":"Sheng F, Gao Y, Wang Y, et al. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(39):e2512274122. doi:<a href=\"https://doi.org/10.1073/pnas.2512274122\">10.1073/pnas.2512274122</a>","ista":"Sheng F, Gao Y, Wang Y, Li Y, Zhang J, Zhang Z, Qin X, Zhang S, Song W, Li J, Guo Y, Friml J, Gong Z, Zhang Q, Zhang J. 2025. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. 122(39), e2512274122.","mla":"Sheng, F., et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 39, National Academy of Sciences, 2025, p. e2512274122, doi:<a href=\"https://doi.org/10.1073/pnas.2512274122\">10.1073/pnas.2512274122</a>."},"doi":"10.1073/pnas.2512274122","file_date_updated":"2025-11-24T13:48:09Z","department":[{"_id":"JiFr"}],"date_published":"2025-09-23T00:00:00Z","external_id":{"pmid":["40986351"],"isi":["001589177800001"]},"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"PlanS_conform":"1","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"project":[{"grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"}],"volume":122,"publication_status":"published","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1073/pnas.2512274122","scopus_import":"1","language":[{"iso":"eng"}],"oa_version":"Published Version","_id":"20635","ddc":["580"],"day":"23","date_created":"2025-11-12T10:03:20Z","file":[{"creator":"dernst","relation":"main_file","success":1,"file_size":2667764,"date_created":"2025-11-24T13:48:09Z","checksum":"38b723a909bf321d7ee537c9d064aa25","content_type":"application/pdf","file_id":"20681","access_level":"open_access","date_updated":"2025-11-24T13:48:09Z","file_name":"2025_PNAS_Sheng.pdf"}],"month":"09","date_updated":"2026-02-16T12:32:51Z","publication":"Proceedings of the National Academy of Sciences","has_accepted_license":"1","title":"Antagonistic SnRK2 and PID kinases' action on auxin transport-mediated root gravitropism","intvolume":"       122","year":"2025","abstract":[{"lang":"eng","text":"Plants have evolved sophisticated mechanisms to adapt to environmental changes, with root gravitropism playing a pivotal role in nutrient and water acquisition. Our study reveals that SnRK2 kinases (SnRK2.2 and SnRK2.3) are critical regulators of root gravitropism through their direct phosphorylation of the auxin transporter PIN2 at S259. We demonstrate that SnRK2s-mediated phosphorylation modulates both the polar localization and transport activity of PIN2. Importantly, SnRK2s function antagonistically to the AGCVIII kinase PID, which phosphorylates PIN2 at a distinct site (S258), establishing a regulatory balance essential for adaptive root growth. Structural modeling and phosphorylation assays further suggest that SnRK2s-mediated phosphorylation at S259 sterically hinders access of PID to S258, providing a mechanistic basis for their antagonistic relationship. These findings uncover a novel regulatory mechanism, by which plants fine-tune root developmental programs to adapt to environmental stimuli, highlighting the evolutionary significance of multilayered kinase-mediated regulation in plant adaptation."}],"OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)","pmid":1,"type":"journal_article","issue":"39","acknowledgement":"This research was funded by Biological Breeding-National Science and Technology Major Project (2023ZD0407201), China Postdoctoral Science Foundation (2024M763575), China Agricultural University Fund (2025RC042), Chinese Universities Scientific Fund (2024RC031), and Austrian Science Fund (FWF; I 6123-B)."},{"external_id":{"pmid":["41218119"]},"publication_identifier":{"eissn":["1091-6490"]},"date_published":"2025-11-18T00:00:00Z","article_type":"original","PlanS_conform":"1","department":[{"_id":"JiFr"}],"file_date_updated":"2025-11-24T09:48:44Z","doi":"10.1073/pnas.2506400122","author":[{"last_name":"Roychoudhry","first_name":"Suruchi","full_name":"Roychoudhry, Suruchi"},{"first_name":"Katelyn","full_name":"Sageman-Furnas, Katelyn","last_name":"Sageman-Furnas"},{"last_name":"Taylor","first_name":"Harry J.","full_name":"Taylor, Harry J."},{"last_name":"Showpnil","full_name":"Showpnil, Iftekhar","first_name":"Iftekhar"},{"last_name":"Wolverton","full_name":"Wolverton, Chris","first_name":"Chris"},{"full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Marta Del","full_name":"Bianco, Marta Del","last_name":"Bianco"},{"last_name":"Kepinski","full_name":"Kepinski, Stefan","first_name":"Stefan"}],"page":"e2506400122","OA_place":"publisher","citation":{"ista":"Roychoudhry S, Sageman-Furnas K, Taylor HJ, Showpnil I, Wolverton C, Friml J, Bianco MD, Kepinski S. 2025. Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. 122(46), e2506400122.","mla":"Roychoudhry, Suruchi, et al. “Angle Dependence as a Unifying Feature of Root Graviresponse Modules.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 46, National Academy of Sciences, 2025, p. e2506400122, doi:<a href=\"https://doi.org/10.1073/pnas.2506400122\">10.1073/pnas.2506400122</a>.","ama":"Roychoudhry S, Sageman-Furnas K, Taylor HJ, et al. Angle dependence as a unifying feature of root graviresponse modules. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(46):e2506400122. doi:<a href=\"https://doi.org/10.1073/pnas.2506400122\">10.1073/pnas.2506400122</a>","apa":"Roychoudhry, S., Sageman-Furnas, K., Taylor, H. J., Showpnil, I., Wolverton, C., Friml, J., … Kepinski, S. (2025). Angle dependence as a unifying feature of root graviresponse modules. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2506400122\">https://doi.org/10.1073/pnas.2506400122</a>","chicago":"Roychoudhry, Suruchi, Katelyn Sageman-Furnas, Harry J. Taylor, Iftekhar Showpnil, Chris Wolverton, Jiří Friml, Marta Del Bianco, and Stefan Kepinski. “Angle Dependence as a Unifying Feature of Root Graviresponse Modules.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2506400122\">https://doi.org/10.1073/pnas.2506400122</a>.","short":"S. Roychoudhry, K. Sageman-Furnas, H.J. Taylor, I. Showpnil, C. Wolverton, J. Friml, M.D. Bianco, S. Kepinski, Proceedings of the National Academy of Sciences 122 (2025) e2506400122.","ieee":"S. Roychoudhry <i>et al.</i>, “Angle dependence as a unifying feature of root graviresponse modules,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 46. National Academy of Sciences, p. e2506400122, 2025."},"quality_controlled":"1","publisher":"National Academy of Sciences","status":"public","ddc":["580"],"day":"18","date_created":"2025-11-23T23:01:38Z","oa_version":"Published Version","language":[{"iso":"eng"}],"scopus_import":"1","_id":"20663","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1073/pnas.2506400122","volume":122,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"oa":1,"publication_status":"published","year":"2025","abstract":[{"lang":"eng","text":"Gravitropism, the patterning of postembryonic growth in relation to the gravity vector, allows plants to optimize the use of limited and nonhomogenous resources in their immediate environment. Since the current model of root gravitropism has not been able to integrate all aspects of the response (perception, response, and behavior), research on gravitropism has been dominated by different theories attempting to conceptualize each aspect individually. In this work, we sought to reevaluate all the main components of the root graviresponse through the lens of angle dependence. We show angle dependence in Cholodny–Went-based auxin asymmetry and growth response, which we tracked back to angle-dependent variation in PIN asymmetry and statolith sedimentation in the columella. Thanks to this approach, we were able to suggest distinct roles for PINs and columella cell tiers, and a potential function for auxin vertical flux through the columella. Our findings provide a unifying framework to further explore the mechanisms that regulate angle-dependent gravitropic response, with major implications of time-dependent features of root graviresponse."}],"title":"Angle dependence as a unifying feature of root graviresponse modules","intvolume":"       122","has_accepted_license":"1","date_updated":"2026-02-16T12:31:31Z","file":[{"file_size":1394055,"date_created":"2025-11-24T09:48:44Z","content_type":"application/pdf","checksum":"5e1c37dddc5db8fbd0128db4a54c4f6b","creator":"dernst","success":1,"relation":"main_file","date_updated":"2025-11-24T09:48:44Z","file_name":"2025_PNAS_Roychoudhry.pdf","file_id":"20676","access_level":"open_access"}],"month":"11","publication":"Proceedings of the National Academy of Sciences","issue":"46","acknowledgement":"This study was funded by the BBSRC (grant no. BB/N010124/1) and Leverhulme Foundation (grant no. RPG-2018-137) to M.D.B. and S.K., a Fully Funded International Research Scholarship awarded to K.S.-F., and by NASA (grant no. 80NSSC21K0585) to C.W.","pmid":1,"type":"journal_article","OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)"},{"article_processing_charge":"No","OA_type":"green","type":"preprint","das_tickbox":"1","acknowledgement":"We deeply appreciate M. Wrzaczek’s constructive input and insightful discussions, which significantly enriched this work. We thank L. Fiedler for helping with the heat map and for the discussions. We also thank the facilities at ISTA, the imaging and optics (IOF) and Lab Support (LSF) facilities for their service and assistance.","publication":"bioRxiv","month":"03","date_updated":"2026-08-14T09:33:45Z","has_accepted_license":"1","title":"TMK interacting network of receptor like kinases for auxin canalization and beyond","related_material":{"record":[{"id":"19395","status":"public","relation":"dissertation_contains"}]},"abstract":[{"lang":"eng","text":"Receptor-like kinases (RLKs), particularly the Transmembrane Kinase (TMK) family, play essential roles in signaling and development, with TMKs being key components of auxin perception and downstream phosphorylation events. While TMKs’ involvement in auxin canalization, a process essential for vasculature formation and regeneration, has been established, nonetheless, the additional signaling and regulatory partners remain poorly understood. In this study, we identify and characterize seven leucine-rich repeat RLKs (TINT1–TINT7) as novel interactors of TMK1, revealing their diverse evolutionary, structural, and functional characteristics. Our results show that TINTs interact with TMK1 and highlight their roles in regulating various developmental processes. Majority of TINTs contributes, together with TMK1, to auxin canalization, with TINT5 linking TMK1 to other canalization component CAMEL. Beyond canalization, we also establish the role of TINT-TMK1 interactions in processes such as stomatal movement and the hypocotyl’s gravitropic response. These findings suggest that TINTs, through their interaction with TMK1, are integral components of various signaling networks, contributing to both auxin canalization and broader plant development."}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.02.28.640727"}],"year":"2025","publication_status":"draft","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"fulldoi":"https://doi.org/10.1101/2025.02.28.640727","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"19398","oa_version":"Published Version","language":[{"iso":"eng"}],"corr_author":"1","day":"02","date_created":"2025-03-12T14:28:53Z","ddc":["580"],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"status":"public","citation":{"mla":"Monzer, Aline, et al. “TMK Interacting Network of Receptor like Kinases for Auxin Canalization and Beyond.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.02.28.640727\">10.1101/2025.02.28.640727</a>.","ista":"Monzer A, Mazur E, Rodriguez Solovey L, Gallei MC, Zou M, Smejkal M, Cervenova E, Friml J. TMK interacting network of receptor like kinases for auxin canalization and beyond. bioRxiv, <a href=\"https://doi.org/10.1101/2025.02.28.640727\">10.1101/2025.02.28.640727</a>.","ama":"Monzer A, Mazur E, Rodriguez Solovey L, et al. TMK interacting network of receptor like kinases for auxin canalization and beyond. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.02.28.640727\">10.1101/2025.02.28.640727</a>","chicago":"Monzer, Aline, Ewa Mazur, Lesia Rodriguez Solovey, Michelle C Gallei, Minxia Zou, Michael Smejkal, Ema Cervenova, and Jiří Friml. “TMK Interacting Network of Receptor like Kinases for Auxin Canalization and Beyond.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.02.28.640727\">https://doi.org/10.1101/2025.02.28.640727</a>.","apa":"Monzer, A., Mazur, E., Rodriguez Solovey, L., Gallei, M. C., Zou, M., Smejkal, M., … Friml, J. (n.d.). TMK interacting network of receptor like kinases for auxin canalization and beyond. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.02.28.640727\">https://doi.org/10.1101/2025.02.28.640727</a>","ieee":"A. Monzer <i>et al.</i>, “TMK interacting network of receptor like kinases for auxin canalization and beyond,” <i>bioRxiv</i>. .","short":"A. Monzer, E. Mazur, L. Rodriguez Solovey, M.C. Gallei, M. Zou, M. Smejkal, E. Cervenova, J. Friml, BioRxiv (n.d.)."},"OA_place":"repository","author":[{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer","first_name":"Aline","full_name":"Monzer, Aline"},{"full_name":"Mazur, Ewa","first_name":"Ewa","last_name":"Mazur"},{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","last_name":"Rodriguez Solovey","orcid":"0000-0002-7244-7237","first_name":"Lesia","full_name":"Rodriguez Solovey, Lesia"},{"first_name":"Michelle C","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei","orcid":"0000-0003-1286-7368"},{"first_name":"Minxia","full_name":"Zou, Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","last_name":"Zou"},{"id":"79a5a1be-04a3-11f0-ba18-a1730e0b58e9","last_name":"Smejkal","first_name":"Michael","full_name":"Smejkal, Michael"},{"last_name":"Cervenova","id":"9f185b95-04a3-11f0-8245-f5e32eeb470f","full_name":"Cervenova, Ema","first_name":"Ema"},{"first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"doi":"10.1101/2025.02.28.640727","department":[{"_id":"GradSch"},{"_id":"JiFr"},{"_id":"EvBe"}],"date_published":"2025-03-02T00:00:00Z"},{"publication_identifier":{"eisbn":["978-3-99078-054-1"],"eissn":["2663-337X"]},"date_published":"2025-03-13T00:00:00Z","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"file_date_updated":"2025-04-01T07:55:27Z","doi":"10.15479/AT-ISTA-19395","degree_awarded":"PhD","citation":{"ama":"Monzer A. Cell-surface auxin signaling: Linking molecular pathways to plant development. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19395\">10.15479/AT-ISTA-19395</a>","mla":"Monzer, Aline. <i>Cell-Surface Auxin Signaling: Linking Molecular Pathways to Plant Development</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19395\">10.15479/AT-ISTA-19395</a>.","ista":"Monzer A. 2025. Cell-surface auxin signaling: Linking molecular pathways to plant development. Institute of Science and Technology Austria.","ieee":"A. Monzer, “Cell-surface auxin signaling: Linking molecular pathways to plant development,” Institute of Science and Technology Austria, 2025.","short":"A. Monzer, Cell-Surface Auxin Signaling: Linking Molecular Pathways to Plant Development, Institute of Science and Technology Austria, 2025.","chicago":"Monzer, Aline. “Cell-Surface Auxin Signaling: Linking Molecular Pathways to Plant Development.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19395\">https://doi.org/10.15479/AT-ISTA-19395</a>.","apa":"Monzer, A. (2025). <i>Cell-surface auxin signaling: Linking molecular pathways to plant development</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19395\">https://doi.org/10.15479/AT-ISTA-19395</a>"},"page":"160","OA_place":"publisher","author":[{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer","first_name":"Aline","full_name":"Monzer, Aline"}],"status":"public","publisher":"Institute of Science and Technology Austria","day":"13","date_created":"2025-03-12T14:25:42Z","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"doi_confirm":"1","ddc":["580"],"_id":"19395","language":[{"iso":"eng"}],"oa_version":"Published Version","corr_author":"1","fulldoi":"https://doi.org/10.15479/AT-ISTA-19395","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","oa":1,"publication_status":"published","abstract":[{"text":"Plant growth and development rely significantly on phytohormones, with auxin serving as a master regulator, orchestrating processes from embryogenesis to organogenesis, vascular patterning, and environmental adaptation. Since its conceptual proposition by Charles Darwin in 1880 as an endogenous chemical signal influencing phototropism in grass, auxin has captivated scientists seeking to understand how such a small molecule exerts a profound influence on plant development.\r\nOne particularly fascinating aspect of auxin function is its ability to self-organize its transport. Through a feedback mechanism between auxin perception and directional transport—primarily mediated by PIN auxin transporters—auxin establishes narrow transport channels. This phenomenon, known as auxin canalization, is fundamental to vascular formation, regeneration, and other key developmental processes. Despite advances in our understanding, driven by experimental studies and computational models, auxin canalization remains an enigma, with many unanswered questions.\r\nLike other hormones, auxin functions through intricate signaling pathways. It operates through at least two distinct signaling mechanisms: the well-characterized canonical pathway and the less understood non-canonical pathway. While significant progress has been made in elucidating the canonical pathway, the non-canonical mechanisms remain less defined and require further investigation.\r\nIn this study, we revisit the non-canonical auxin signaling pathway mediated by the cell-surface complex Auxin Binding Protein 1-Transmembrane Kinase 1 (ABP1-TMK1), with a particular focus on its downstream phosphorylation events. We reveal that this auxin-mediated phosphorylation is conserved across the green lineage, underscoring its fundamental role in plant development. We explore key phosphorylation targets, particularly PIN2, which is essential for root gravitropism. To further understand TMK1’s role in diverse developmental processes, we identified and investigated its interactors as potential co-receptors or regulatory components within its signaling network.\r\nGiven the previously established role of ABP1-TMK1 in auxin canalization, we sought to further investigate this process and identified several TMK1 interactors also involved in this intricate mechanism.\r\nThese findings provide new insights into the complex regulation of auxin canalization, highlighting a broader and more interconnected signaling framework than previously understood.","lang":"eng"}],"year":"2025","title":"Cell-surface auxin signaling: Linking molecular pathways to plant development","has_accepted_license":"1","related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"12291"},{"id":"19399","relation":"part_of_dissertation","status":"public"},{"id":"19398","status":"public","relation":"part_of_dissertation"},{"relation":"part_of_dissertation","status":"public","id":"14826"}]},"date_updated":"2026-10-15T08:06:16Z","alternative_title":["ISTA Thesis"],"file":[{"file_id":"19396","access_level":"open_access","date_updated":"2025-03-12T14:14:49Z","file_name":"Final Thesis Aline Monzer.pdf","success":1,"relation":"main_file","creator":"amonzer","checksum":"9a3dd03bb4ec6b9907a325c3c4e8a1d7","content_type":"application/pdf","file_size":13119670,"date_created":"2025-03-12T14:14:49Z"},{"creator":"amonzer","relation":"source_file","file_size":13774837,"date_created":"2025-03-12T14:15:19Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"a353ce1ee2eabce37bca35499e76dbf1","file_id":"19397","access_level":"closed","date_updated":"2025-04-01T07:55:27Z","file_name":"Thesis Aline.docx"}],"month":"03","acknowledgement":"I would like to acknowledge the facilities at ISTA, particularly LSF, IOF, and, of course, the plant facility, for providing the necessary resources for my research.","supervisor":[{"orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří"}],"type":"dissertation","article_processing_charge":"No"},{"doi":"10.1016/j.celrep.2025.116024","file_date_updated":"2025-07-22T08:52:17Z","department":[{"_id":"JiFr"}],"date_published":"2025-07-22T00:00:00Z","external_id":{"isi":["001533244800001"],"pmid":["40668679"]},"publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"article_type":"original","publisher":"Cell Press","status":"public","OA_place":"publisher","author":[{"last_name":"Guan","id":"56aad729-cca2-11ed-a45a-9b4138991a48","full_name":"Guan, Bin","first_name":"Bin"},{"first_name":"Ke Xuan","full_name":"Xie, Ke Xuan","last_name":"Xie"},{"first_name":"Xin Qiao","full_name":"Du, Xin Qiao","last_name":"Du"},{"last_name":"Bai","first_name":"Yu Xuan","full_name":"Bai, Yu Xuan"},{"first_name":"Peng Chao","full_name":"Hao, Peng Chao","last_name":"Hao"},{"last_name":"Lin","full_name":"Lin, Wen Hui","first_name":"Wen Hui"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří"},{"last_name":"Xue","first_name":"Hong Wei","full_name":"Xue, Hong Wei"}],"quality_controlled":"1","isi":1,"citation":{"mla":"Guan, Bin, et al. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” <i>Cell Reports</i>, vol. 44, no. 7, 116024, Cell Press, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116024\">10.1016/j.celrep.2025.116024</a>.","ista":"Guan B, Xie KX, Du XQ, Bai YX, Hao PC, Lin WH, Friml J, Xue HW. 2025. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. 44(7), 116024.","ama":"Guan B, Xie KX, Du XQ, et al. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. <i>Cell Reports</i>. 2025;44(7). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116024\">10.1016/j.celrep.2025.116024</a>","chicago":"Guan, Bin, Ke Xuan Xie, Xin Qiao Du, Yu Xuan Bai, Peng Chao Hao, Wen Hui Lin, Jiří Friml, and Hong Wei Xue. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” <i>Cell Reports</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.116024\">https://doi.org/10.1016/j.celrep.2025.116024</a>.","apa":"Guan, B., Xie, K. X., Du, X. Q., Bai, Y. X., Hao, P. C., Lin, W. H., … Xue, H. W. (2025). Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2025.116024\">https://doi.org/10.1016/j.celrep.2025.116024</a>","ieee":"B. Guan <i>et al.</i>, “Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD,” <i>Cell Reports</i>, vol. 44, no. 7. Cell Press, 2025.","short":"B. Guan, K.X. Xie, X.Q. Du, Y.X. Bai, P.C. Hao, W.H. Lin, J. Friml, H.W. Xue, Cell Reports 44 (2025)."},"scopus_import":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"_id":"20029","ddc":["580"],"date_created":"2025-07-20T22:02:01Z","day":"22","tmp":{"short":"CC BY-NC (4.0)","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"volume":44,"oa":1,"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1016/j.celrep.2025.116024","has_accepted_license":"1","intvolume":"        44","title":"Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD","year":"2025","abstract":[{"lang":"eng","text":"Vacuolar acidification is crucial for the homeostasis of intracellular pH and the recycling of proteins and nutrients in cells, thereby playing important roles in various physiological processes related to vacuolar function. The key factors regulating vacuolar acidification and underlying mechanisms remain unclear. Here, we report that Arabidopsis phospholipase Dζ2 (PLDζ2) promotes the acidification of the vacuolar lumen to stimulate autophagic degradation under phosphorus deficiency. The pldζ2 mutant massively accumulates autophagic structures while exhibiting premature leaf senescence under nutrient starvation. Impaired autophagic flux, lytic vacuole morphology, and lytic degradation in pldζ2 indicate that PLDζ2 regulates autophagy by affecting the vacuolar function. PLDζ2 locates in both tonoplast and cytoplasm. Genetic, structural, and biochemical studies demonstrate that PLDζ2 directly interacts with vacuolar-type ATPase (V-ATPase) subunit D (VATD) to promote vacuolar acidification and autophagy under phosphorus starvation. These findings reveal the importance of V-ATPase and vacuolar pH in autophagic activity and provide clues in elucidating the regulatory mechanism of vacuolar acidification."}],"file":[{"date_updated":"2025-07-22T08:52:17Z","file_name":"2025_CellReports_Guan.pdf","file_id":"20067","access_level":"open_access","file_size":37708120,"date_created":"2025-07-22T08:52:17Z","content_type":"application/pdf","checksum":"ee03deee47a084b0295251dc49470ad4","creator":"dernst","success":1,"relation":"main_file"}],"month":"07","date_updated":"2026-10-20T08:22:53Z","publication":"Cell Reports","article_number":"116024","issue":"7","acknowledgement":"The study was supported by National Natural Science Foundation of China (NSFC, 92354301, 32230011, 32200274, and 91954206). The computations were run on the Siyuan-1 cluster supported by the Center for High-Performance Computing at Shanghai Jiao Tong University.","OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)","pmid":1,"type":"journal_article"}]