[{"publication_status":"published","article_processing_charge":"No","quality_controlled":"1","citation":{"short":"P. Žádníková, K.T. Wabnik, A. Abuzeineh, M. Gallemí, D. Van Der Straeten, R. Smith, D. Inze, J. Friml, P. Prusinkiewicz, E. Benková, Plant Cell 28 (2016) 2464–2477.","ama":"Žádníková P, Wabnik KT, Abuzeineh A, et al. A model of differential growth guided apical hook formation in plants. <i>Plant Cell</i>. 2016;28(10):2464-2477. doi:<a href=\"https://doi.org/10.1105/tpc.15.00569\">10.1105/tpc.15.00569</a>","chicago":"Žádníková, Petra, Krzysztof T Wabnik, Anas Abuzeineh, Marçal Gallemí, Dominique Van Der Straeten, Richard Smith, Dirk Inze, Jiří Friml, Przemysław Prusinkiewicz, and Eva Benková. “A Model of Differential Growth Guided Apical Hook Formation in Plants.” <i>Plant Cell</i>. American Society of Plant Biologists, 2016. <a href=\"https://doi.org/10.1105/tpc.15.00569\">https://doi.org/10.1105/tpc.15.00569</a>.","mla":"Žádníková, Petra, et al. “A Model of Differential Growth Guided Apical Hook Formation in Plants.” <i>Plant Cell</i>, vol. 28, no. 10, American Society of Plant Biologists, 2016, pp. 2464–77, doi:<a href=\"https://doi.org/10.1105/tpc.15.00569\">10.1105/tpc.15.00569</a>.","apa":"Žádníková, P., Wabnik, K. T., Abuzeineh, A., Gallemí, M., Van Der Straeten, D., Smith, R., … Benková, E. (2016). A model of differential growth guided apical hook formation in plants. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.15.00569\">https://doi.org/10.1105/tpc.15.00569</a>","ieee":"P. Žádníková <i>et al.</i>, “A model of differential growth guided apical hook formation in plants,” <i>Plant Cell</i>, vol. 28, no. 10. American Society of Plant Biologists, pp. 2464–2477, 2016.","ista":"Žádníková P, Wabnik KT, Abuzeineh A, Gallemí M, Van Der Straeten D, Smith R, Inze D, Friml J, Prusinkiewicz P, Benková E. 2016. A model of differential growth guided apical hook formation in plants. Plant Cell. 28(10), 2464–2477."},"isi":1,"project":[{"call_identifier":"FP7","grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis","_id":"253FCA6A-B435-11E9-9278-68D0E5697425"}],"oa":1,"issue":"10","oa_version":"Submitted Version","ec_funded":1,"title":"A model of differential growth guided apical hook formation in plants","month":"10","language":[{"iso":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.1105/tpc.15.00569","date_created":"2018-12-11T11:50:26Z","scopus_import":"1","volume":28,"type":"journal_article","page":"2464 - 2477","year":"2016","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134968/"}],"publisher":"American Society of Plant Biologists","abstract":[{"text":"Differential cell growth enables flexible organ bending in the presence of environmental signals such as light or gravity. A prominent example of the developmental processes based on differential cell growth is the formation of the apical hook that protects the fragile shoot apical meristem when it breaks through the soil during germination. Here, we combined in silico and in vivo approaches to identify a minimal mechanism producing auxin gradient-guided differential growth during the establishment of the apical hook in the model plant Arabidopsis thaliana. Computer simulation models based on experimental data demonstrate that asymmetric expression of the PIN-FORMED auxin efflux carrier at the concave (inner) versus convex (outer) side of the hook suffices to establish an auxin maximum in the epidermis at the concave side of the apical hook. Furthermore, we propose a mechanism that translates this maximum into differential growth, and thus curvature, of the apical hook. Through a combination of experimental and in silico computational approaches, we have identified the individual contributions of differential cell elongation and proliferation to defining the apical hook and reveal the role of auxin-ethylene crosstalk in balancing these two processes. © 2016 American Society of Plant Biologists. All rights reserved.","lang":"eng"}],"author":[{"last_name":"Žádníková","full_name":"Žádníková, Petra","first_name":"Petra"},{"full_name":"Wabnik, Krzysztof T","first_name":"Krzysztof T","orcid":"0000-0001-7263-0560","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","last_name":"Wabnik"},{"last_name":"Abuzeineh","first_name":"Anas","full_name":"Abuzeineh, Anas"},{"first_name":"Marçal","full_name":"Gallemí, Marçal","last_name":"Gallemí"},{"last_name":"Van Der Straeten","full_name":"Van Der Straeten, Dominique","first_name":"Dominique"},{"first_name":"Richard","full_name":"Smith, Richard","last_name":"Smith"},{"full_name":"Inze, Dirk","first_name":"Dirk","last_name":"Inze"},{"first_name":"Jirí","full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Prusinkiewicz","full_name":"Prusinkiewicz, Przemysław","first_name":"Przemysław"},{"full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"}],"_id":"1153","corr_author":"1","acknowledgement":"We thank Martine De Cock and Annick Bleys for help in preparing the manuscript, Daniel Van Damme for sharing material and stimulating discussion, and Rudiger Simon for support during revision of the manuscript.\r\nThis work was supported by grants from the European Research Council (StartingIndependentResearchGrantERC-2007-Stg-207362-HCPO)and the Czech Science Foundation (GACR CZ.1.07/2.3.00/20.0043) to E.B.\r\nand Natural Sciences and Engineering Research Council of Canada Discovery Grant 2014-05325 to P.P. K.W. acknowledges funding from a Human Frontier Science Program Long-Term Fellowship (LT-000209-2014).","publication":"Plant Cell","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"intvolume":"        28","day":"01","date_published":"2016-10-01T00:00:00Z","publist_id":"6205","external_id":{"isi":["000390135400013"]},"date_updated":"2025-09-22T09:56:45Z","status":"public"},{"has_accepted_license":"1","issue":"22","oa_version":"Submitted Version","oa":1,"month":"11","title":"Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity","ec_funded":1,"language":[{"iso":"eng"}],"publication_status":"published","quality_controlled":"1","article_processing_charge":"No","project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300"}],"isi":1,"citation":{"chicago":"Rakusová, Hana, Mohamad Abbas, Huibin Han, Siyuan Song, Hélène Robert, and Jiří Friml. “Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity.” <i>Current Biology</i>. Cell Press, 2016. <a href=\"https://doi.org/10.1016/j.cub.2016.08.067\">https://doi.org/10.1016/j.cub.2016.08.067</a>.","mla":"Rakusová, Hana, et al. “Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity.” <i>Current Biology</i>, vol. 26, no. 22, Cell Press, 2016, pp. 3026–32, doi:<a href=\"https://doi.org/10.1016/j.cub.2016.08.067\">10.1016/j.cub.2016.08.067</a>.","ista":"Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. 2016. Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity. Current Biology. 26(22), 3026–3032.","apa":"Rakusová, H., Abbas, M., Han, H., Song, S., Robert, H., &#38; Friml, J. (2016). Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2016.08.067\">https://doi.org/10.1016/j.cub.2016.08.067</a>","ieee":"H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, and J. Friml, “Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity,” <i>Current Biology</i>, vol. 26, no. 22. Cell Press, pp. 3026–3032, 2016.","short":"H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, J. Friml, Current Biology 26 (2016) 3026–3032.","ama":"Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity. <i>Current Biology</i>. 2016;26(22):3026-3032. doi:<a href=\"https://doi.org/10.1016/j.cub.2016.08.067\">10.1016/j.cub.2016.08.067</a>"},"doi":"10.1016/j.cub.2016.08.067","date_created":"2018-12-11T11:50:44Z","volume":26,"scopus_import":"1","type":"journal_article","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"1212","pubrep_id":"1008","acknowledgement":"We thank Dr. Jie Li (Key Laboratory of Plant Molecular Physiology, Chinese Academy of Science, China) for the pPIN3::PIN3-GFP/DII::VENUS line and Martine De Cock for help in preparing the manuscript. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP), by the Czech Science Foundation GAČR (GA13-40637S) to J.F., and by the Ministry of Education, Youth and Sports of the Czech Republic under the project CEITEC 2020 (LQ1601) to H.S.R. H.R. is indebted to the Agency for Innovation by Science and Technology (IWT) for a predoctoral fellowship.\r\n","file":[{"relation":"main_file","file_name":"IST-2018-1008-v1+1_Rakusova_CurrBiol_2016_proof.pdf","creator":"system","date_created":"2018-12-12T10:09:33Z","checksum":"79ed2498185a027cf51a8f88100379e6","file_id":"4757","content_type":"application/pdf","access_level":"open_access","date_updated":"2020-07-14T12:44:39Z","file_size":5391923}],"year":"2016","page":"3026 - 3032","file_date_updated":"2020-07-14T12:44:39Z","publisher":"Cell Press","author":[{"last_name":"Rakusová","full_name":"Rakusová, Hana","first_name":"Hana"},{"id":"47E8FC1C-F248-11E8-B48F-1D18A9856A87","last_name":"Abbas","first_name":"Mohamad","full_name":"Abbas, Mohamad"},{"last_name":"Han","id":"31435098-F248-11E8-B48F-1D18A9856A87","full_name":"Han, Huibin","first_name":"Huibin"},{"last_name":"Song","first_name":"Siyuan","full_name":"Song, Siyuan"},{"last_name":"Robert","full_name":"Robert, Hélène","first_name":"Hélène"},{"first_name":"Jirí","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml"}],"abstract":[{"lang":"eng","text":"Plants adjust their growth according to gravity. Gravitropism involves gravity perception, signal transduction, and asymmetric growth response, with organ bending as a consequence [1]. Asymmetric growth results from the asymmetric distribution of the plant-specific signaling molecule auxin [2] that is generated by lateral transport, mediated in the hypocotyl predominantly by the auxin transporter PIN-FORMED3 (PIN3) [3–5]. Gravity stimulation polarizes PIN3 to the bottom sides of endodermal cells, correlating with increased auxin accumulation in adjacent tissues at the lower side of the stimulated organ, where auxin induces cell elongation and, hence, organ bending. A curvature response allows the hypocotyl to resume straight growth at a defined angle [6], implying that at some point auxin symmetry is restored to prevent overbending. Here, we present initial insights into cellular and molecular mechanisms that lead to the termination of the tropic response. We identified an auxin feedback on PIN3 polarization as underlying mechanism that restores symmetry of the PIN3-dependent auxin flow. Thus, two mechanistically distinct PIN3 polarization events redirect auxin fluxes at different time points of the gravity response: first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower hypocotyl side, where auxin gradually accumulates and promotes growth, and later PIN3 polarization to the opposite cell side, depleting this auxin maximum to end the bending. Accordingly, genetic or pharmacological interference with the late PIN3 polarization prevents termination of the response and leads to hypocotyl overbending. This observation reveals a role of auxin feedback on PIN polarity in the termination of the tropic response. © 2016 Elsevier Ltd"}],"intvolume":"        26","day":"21","publist_id":"6138","date_published":"2016-11-21T00:00:00Z","external_id":{"isi":["000388545900020"]},"date_updated":"2026-04-28T08:29:26Z","status":"public","ddc":["581"],"department":[{"_id":"JiFr"}],"publication":"Current Biology"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","article_type":"original","scopus_import":"1","volume":5,"date_created":"2018-12-11T11:50:47Z","doi":"10.12688/f1000research.7654.1","citation":{"ama":"Michalko J, Glanc M, Perrot Rechenmann C, Friml J. Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein. <i>F1000 Research </i>. 2016;5. doi:<a href=\"https://doi.org/10.12688/f1000research.7654.1\">10.12688/f1000research.7654.1</a>","short":"J. Michalko, M. Glanc, C. Perrot Rechenmann, J. Friml, F1000 Research  5 (2016).","ista":"Michalko J, Glanc M, Perrot Rechenmann C, Friml J. 2016. Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein. F1000 Research . 5, 86.","apa":"Michalko, J., Glanc, M., Perrot Rechenmann, C., &#38; Friml, J. (2016). Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein. <i>F1000 Research </i>. F1000 Research. <a href=\"https://doi.org/10.12688/f1000research.7654.1\">https://doi.org/10.12688/f1000research.7654.1</a>","ieee":"J. Michalko, M. Glanc, C. Perrot Rechenmann, and J. Friml, “Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein,” <i>F1000 Research </i>, vol. 5. F1000 Research, 2016.","chicago":"Michalko, Jaroslav, Matous Glanc, Catherine Perrot Rechenmann, and Jiří Friml. “Strong Morphological Defects in Conditional Arabidopsis Abp1 Knock-down Mutants Generated in Absence of Functional ABP1 Protein.” <i>F1000 Research </i>. F1000 Research, 2016. <a href=\"https://doi.org/10.12688/f1000research.7654.1\">https://doi.org/10.12688/f1000research.7654.1</a>.","mla":"Michalko, Jaroslav, et al. “Strong Morphological Defects in Conditional Arabidopsis Abp1 Knock-down Mutants Generated in Absence of Functional ABP1 Protein.” <i>F1000 Research </i>, vol. 5, 86, F1000 Research, 2016, doi:<a href=\"https://doi.org/10.12688/f1000research.7654.1\">10.12688/f1000research.7654.1</a>."},"project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300"}],"quality_controlled":"1","article_processing_charge":"No","publication_status":"published","language":[{"iso":"eng"}],"ec_funded":1,"month":"01","title":"Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein","oa":1,"oa_version":"Published Version","has_accepted_license":"1","department":[{"_id":"JiFr"}],"publication":"F1000 Research ","ddc":["581"],"tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"status":"public","date_updated":"2025-04-15T07:48:02Z","date_published":"2016-01-20T00:00:00Z","publist_id":"6113","intvolume":"         5","day":"20","abstract":[{"lang":"eng","text":"The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in plants. Since decades ago, it has been the prime receptor candidate for the plant hormone auxin with a plethora of described functions in auxin signaling and development. The developmental importance of ABP1 has recently been questioned by identification of Arabidopsis thaliana abp1 knock-out alleles that show no obvious phenotypes under normal growth conditions. In this study, we examined the contradiction between the normal growth and development of the abp1 knock-outs and the strong morphological defects observed in three different ethanol-inducible abp1 knock-down mutants ( abp1-AS, SS12K, SS12S). By analyzing segregating populations of abp1 knock-out vs. abp1 knock-down crosses we show that the strong morphological defects that were believed to be the result of conditional down-regulation of ABP1 can be reproduced also in the absence of the functional ABP1 protein. This data suggests that the phenotypes in abp1 knock-down lines are due to the off-target effects and asks for further reflections on the biological function of ABP1 or alternative explanations for the missing phenotypic defects in the abp1 loss-of-function alleles."}],"author":[{"full_name":"Michalko, Jaroslav","first_name":"Jaroslav","id":"483727CA-F248-11E8-B48F-1D18A9856A87","last_name":"Michalko"},{"orcid":"0000-0003-0619-7783","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","last_name":"Glanc","full_name":"Glanc, Matous","first_name":"Matous"},{"last_name":"Perrot Rechenmann","full_name":"Perrot Rechenmann, Catherine","first_name":"Catherine"},{"first_name":"Jirí","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml"}],"publisher":"F1000 Research","file_date_updated":"2020-07-14T12:44:39Z","year":"2016","acknowledgement":"This work was supported by ERC Independent Research grant (ERC-2011-StG-20101109-PSDP to JF). JM internship was supported by the grant “Action Austria – Slovakia”. MG was supported by the scholarship \"Stipendien der Stipendienstiftung der Republik Österreich\". Work by EH and CPR were supported by ANR blanc ANR-14-CE11-0018. We would like to thank Mark Estelle and Yunde Zhao for provid\r\n-\r\ning \r\nabp1-c1\r\n, \r\nabp1-TD1 \r\nand \r\nabp1-WTc1 \r\nseeds. We thank Emeline \r\nHuault for technical assistance.","file":[{"access_level":"open_access","content_type":"application/pdf","date_updated":"2020-07-14T12:44:39Z","file_size":2990459,"checksum":"c9e50bb6096a7ba4a832969935820f19","creator":"system","date_created":"2018-12-12T10:15:33Z","file_id":"5154","file_name":"IST-2016-711-v1+1_770cf1e0-612f-4e85-a500-54b6349fbbab_7654_-_jaroslav_michalko.pdf","relation":"main_file"}],"pubrep_id":"711","_id":"1221","article_number":"86"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:50:53Z","doi":"10.3389/fpls.2015.01262","type":"journal_article","scopus_import":"1","volume":6,"publication_status":"published","citation":{"ista":"von Wangenheim D, Rosero A, Komis G, Šamajová O, Ovečka M, Voigt B, Šamaj J. 2016. Endosomal interactions during root hair growth. Frontiers in Plant Science. 6(JAN2016), 1262.","apa":"von Wangenheim, D., Rosero, A., Komis, G., Šamajová, O., Ovečka, M., Voigt, B., &#38; Šamaj, J. (2016). Endosomal interactions during root hair growth. <i>Frontiers in Plant Science</i>. Frontiers Research Foundation. <a href=\"https://doi.org/10.3389/fpls.2015.01262\">https://doi.org/10.3389/fpls.2015.01262</a>","ieee":"D. von Wangenheim <i>et al.</i>, “Endosomal interactions during root hair growth,” <i>Frontiers in Plant Science</i>, vol. 6, no. JAN2016. Frontiers Research Foundation, 2016.","mla":"von Wangenheim, Daniel, et al. “Endosomal Interactions during Root Hair Growth.” <i>Frontiers in Plant Science</i>, vol. 6, no. JAN2016, 1262, Frontiers Research Foundation, 2016, doi:<a href=\"https://doi.org/10.3389/fpls.2015.01262\">10.3389/fpls.2015.01262</a>.","chicago":"Wangenheim, Daniel von, Amparo Rosero, George Komis, Olga Šamajová, Miroslav Ovečka, Boris Voigt, and Jozef Šamaj. “Endosomal Interactions during Root Hair Growth.” <i>Frontiers in Plant Science</i>. Frontiers Research Foundation, 2016. <a href=\"https://doi.org/10.3389/fpls.2015.01262\">https://doi.org/10.3389/fpls.2015.01262</a>.","ama":"von Wangenheim D, Rosero A, Komis G, et al. Endosomal interactions during root hair growth. <i>Frontiers in Plant Science</i>. 2016;6(JAN2016). doi:<a href=\"https://doi.org/10.3389/fpls.2015.01262\">10.3389/fpls.2015.01262</a>","short":"D. von Wangenheim, A. Rosero, G. Komis, O. Šamajová, M. Ovečka, B. Voigt, J. Šamaj, Frontiers in Plant Science 6 (2016)."},"isi":1,"article_processing_charge":"No","quality_controlled":"1","oa":1,"issue":"JAN2016","oa_version":"Published Version","has_accepted_license":"1","language":[{"iso":"eng"}],"month":"01","title":"Endosomal interactions during root hair growth","publication":"Frontiers in Plant Science","department":[{"_id":"JiFr"}],"date_published":"2016-01-29T00:00:00Z","publist_id":"6094","intvolume":"         6","day":"29","ddc":["581"],"tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"status":"public","external_id":{"isi":["000368891500001"]},"date_updated":"2025-09-22T09:18:11Z","file_date_updated":"2020-07-14T12:44:41Z","year":"2016","abstract":[{"lang":"eng","text":"The dynamic localization of endosomal compartments labeled with targeted fluorescent protein tags is routinely followed by time lapse fluorescence microscopy approaches and single particle tracking algorithms. In this way trajectories of individual endosomes can be mapped and linked to physiological processes as cell growth. However, other aspects of dynamic behavior including endosomal interactions are difficult to follow in this manner. Therefore, we characterized the localization and dynamic properties of early and late endosomes throughout the entire course of root hair formation by means of spinning disc time lapse imaging and post-acquisition automated multitracking and quantitative analysis. Our results show differential motile behavior of early and late endosomes and interactions of late endosomes that may be specified to particular root hair domains. Detailed data analysis revealed a particular transient interaction between late endosomes—termed herein as dancing-endosomes—which is not concluding to vesicular fusion. Endosomes preferentially located in the root hair tip interacted as dancing-endosomes and traveled short distances during this interaction. Finally, sizes of early and late endosomes were addressed by means of super-resolution structured illumination microscopy (SIM) to corroborate measurements on the spinning disc. This is a first study providing quantitative microscopic data on dynamic spatio-temporal interactions of endosomes during root hair tip growth."}],"author":[{"first_name":"Daniel","full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6862-1247"},{"last_name":"Rosero","full_name":"Rosero, Amparo","first_name":"Amparo"},{"first_name":"George","full_name":"Komis, George","last_name":"Komis"},{"last_name":"Šamajová","full_name":"Šamajová, Olga","first_name":"Olga"},{"last_name":"Ovečka","first_name":"Miroslav","full_name":"Ovečka, Miroslav"},{"full_name":"Voigt, Boris","first_name":"Boris","last_name":"Voigt"},{"last_name":"Šamaj","first_name":"Jozef","full_name":"Šamaj, Jozef"}],"publisher":"Frontiers Research Foundation","_id":"1238","article_number":"1262","acknowledgement":"This work was supported by National Program for Sustainability I (grant no. LO1204) provided by the Czech Ministry of Education and by Institutional Fund of Palacký University Olomouc (GK and OŠ).\r\nWe thank Sabine Fischer for help with the statistics.","file":[{"file_name":"IST-2016-710-v1+1_fpls-06-01262.pdf","relation":"main_file","content_type":"application/pdf","access_level":"open_access","date_updated":"2020-07-14T12:44:41Z","file_size":1640550,"creator":"system","date_created":"2018-12-12T10:09:36Z","checksum":"3127eab844d53564bf47e2b6b42f1ca0","file_id":"4760"}],"pubrep_id":"710"},{"project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7"}],"isi":1,"citation":{"chicago":"Karampelias, Michael, Pia Neyt, Steven De Groeve, Stijn Aesaert, Griet Coussens, Jakub Rolčík, Leonardo Bruno, et al. “ROTUNDA3 Function in Plant Development by Phosphatase 2A-Mediated Regulation of Auxin Transporter Recycling.” <i>PNAS</i>. National Academy of Sciences, 2016. <a href=\"https://doi.org/10.1073/pnas.1501343112\">https://doi.org/10.1073/pnas.1501343112</a>.","mla":"Karampelias, Michael, et al. “ROTUNDA3 Function in Plant Development by Phosphatase 2A-Mediated Regulation of Auxin Transporter Recycling.” <i>PNAS</i>, vol. 113, no. 10, National Academy of Sciences, 2016, pp. 2768–73, doi:<a href=\"https://doi.org/10.1073/pnas.1501343112\">10.1073/pnas.1501343112</a>.","ista":"Karampelias M, Neyt P, De Groeve S, Aesaert S, Coussens G, Rolčík J, Bruno L, De Winne N, Van Minnebruggen A, Van Montagu M, Ponce M, Micol J, Friml J, De Jaeger G, Van Lijsebettens M. 2016. ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation of auxin transporter recycling. PNAS. 113(10), 2768–2773.","apa":"Karampelias, M., Neyt, P., De Groeve, S., Aesaert, S., Coussens, G., Rolčík, J., … Van Lijsebettens, M. (2016). ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation of auxin transporter recycling. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1501343112\">https://doi.org/10.1073/pnas.1501343112</a>","ieee":"M. Karampelias <i>et al.</i>, “ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation of auxin transporter recycling,” <i>PNAS</i>, vol. 113, no. 10. National Academy of Sciences, pp. 2768–2773, 2016.","short":"M. Karampelias, P. Neyt, S. De Groeve, S. Aesaert, G. Coussens, J. Rolčík, L. Bruno, N. De Winne, A. Van Minnebruggen, M. Van Montagu, M. Ponce, J. Micol, J. Friml, G. De Jaeger, M. Van Lijsebettens, PNAS 113 (2016) 2768–2773.","ama":"Karampelias M, Neyt P, De Groeve S, et al. ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation of auxin transporter recycling. <i>PNAS</i>. 2016;113(10):2768-2773. doi:<a href=\"https://doi.org/10.1073/pnas.1501343112\">10.1073/pnas.1501343112</a>"},"article_processing_charge":"No","quality_controlled":"1","publication_status":"published","language":[{"iso":"eng"}],"ec_funded":1,"month":"03","title":"ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation of auxin transporter recycling","oa_version":"Submitted Version","issue":"10","oa":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","volume":113,"scopus_import":"1","date_created":"2018-12-11T11:50:56Z","doi":"10.1073/pnas.1501343112","author":[{"first_name":"Michael","full_name":"Karampelias, Michael","last_name":"Karampelias"},{"last_name":"Neyt","first_name":"Pia","full_name":"Neyt, Pia"},{"first_name":"Steven","full_name":"De Groeve, Steven","last_name":"De Groeve"},{"last_name":"Aesaert","first_name":"Stijn","full_name":"Aesaert, Stijn"},{"full_name":"Coussens, Griet","first_name":"Griet","last_name":"Coussens"},{"last_name":"Rolčík","first_name":"Jakub","full_name":"Rolčík, Jakub"},{"last_name":"Bruno","first_name":"Leonardo","full_name":"Bruno, Leonardo"},{"first_name":"Nancy","full_name":"De Winne, Nancy","last_name":"De Winne"},{"full_name":"Van Minnebruggen, Annemie","first_name":"Annemie","last_name":"Van Minnebruggen"},{"last_name":"Van Montagu","full_name":"Van Montagu, Marc","first_name":"Marc"},{"first_name":"Maria","full_name":"Ponce, Maria","last_name":"Ponce"},{"full_name":"Micol, José","first_name":"José","last_name":"Micol"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí"},{"last_name":"De Jaeger","first_name":"Geert","full_name":"De Jaeger, Geert"},{"first_name":"Mieke","full_name":"Van Lijsebettens, Mieke","last_name":"Van Lijsebettens"}],"abstract":[{"text":"The shaping of organs in plants depends on the intercellular flow of the phytohormone auxin, of which the directional signaling is determined by the polar subcellular localization of PIN-FORMED (PIN) auxin transport proteins. Phosphorylation dynamics of PIN proteins are affected by the protein phosphatase 2A (PP2A) and the PINOID kinase, which act antagonistically to mediate their apical-basal polar delivery. Here, we identified the ROTUNDA3 (RON3) protein as a regulator of the PP2A phosphatase activity in Arabidopsis thaliana. The RON3 gene was map-based cloned starting from the ron3-1 leaf mutant and found to be a unique, plant-specific gene coding for a protein with high and dispersed proline content. The ron3-1 and ron3-2 mutant phenotypes [i.e., reduced apical dominance, primary root length, lateral root emergence, and growth; increased ectopic stages II, IV, and V lateral root primordia; decreased auxin maxima in indole-3-acetic acid (IAA)-treated root apical meristems; hypergravitropic root growth and response; increased IAA levels in shoot apices; and reduced auxin accumulation in root meristems] support a role for RON3 in auxin biology. The affinity-purified PP2A complex with RON3 as bait suggested that RON3 might act in PIN transporter trafficking. Indeed, pharmacological interference with vesicle trafficking processes revealed that single ron3-2 and double ron3-2 rcn1 mutants have altered PIN polarity and endocytosis in specific cells. Our data indicate that RON3 contributes to auxin-mediated development by playing a role in PIN recycling and polarity establishment through regulation of the PP2A complex activity.","lang":"eng"}],"publisher":"National Academy of Sciences","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791031/","open_access":"1"}],"year":"2016","page":"2768 - 2773","acknowledgement":"This work was supported by the Ghent University Special Research Fund (M.K.), the European Research Council (Project ERC-2011-StG-20101109-PSDP) (to J.F.), and the Körber European Science Foun-\r\ndation (J.F.). S.D.G. is indebted to the Agency for Science and Technology for\r\na predoctoral fellowship.","_id":"1247","publication":"PNAS","department":[{"_id":"JiFr"}],"status":"public","date_updated":"2025-09-22T09:13:28Z","external_id":{"isi":["000372013300055"]},"publist_id":"6081","date_published":"2016-03-08T00:00:00Z","day":"08","intvolume":"       113"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.1105/tpc.15.00726","date_created":"2018-12-11T11:50:57Z","scopus_import":"1","volume":28,"type":"journal_article","publication_status":"published","quality_controlled":"1","article_processing_charge":"No","citation":{"mla":"Zhu, Jinsheng, et al. “TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics.” <i>Plant Cell</i>, vol. 28, no. 4, American Society of Plant Biologists, 2016, pp. 930–48, doi:<a href=\"https://doi.org/10.1105/tpc.15.00726\">10.1105/tpc.15.00726</a>.","chicago":"Zhu, Jinsheng, Aurélien Bailly, Marta Zwiewka, Valpuri Sovero, Martin Di Donato, Pei Ge, Jacqueline Oehri, et al. “TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics.” <i>Plant Cell</i>. American Society of Plant Biologists, 2016. <a href=\"https://doi.org/10.1105/tpc.15.00726\">https://doi.org/10.1105/tpc.15.00726</a>.","apa":"Zhu, J., Bailly, A., Zwiewka, M., Sovero, V., Di Donato, M., Ge, P., … Geisler, M. (2016). TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.15.00726\">https://doi.org/10.1105/tpc.15.00726</a>","ieee":"J. Zhu <i>et al.</i>, “TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics,” <i>Plant Cell</i>, vol. 28, no. 4. American Society of Plant Biologists, pp. 930–948, 2016.","ista":"Zhu J, Bailly A, Zwiewka M, Sovero V, Di Donato M, Ge P, Oehri J, Aryal B, Hao P, Linnert M, Burgardt N, Lücke C, Weiwad M, Michel M, Weiergräber O, Pollmann S, Azzarello E, Mancuso S, Ferro N, Fukao Y, Hoffmann C, Wedlich Söldner R, Friml J, Thomas C, Geisler M. 2016. TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics. Plant Cell. 28(4), 930–948.","short":"J. Zhu, A. Bailly, M. Zwiewka, V. Sovero, M. Di Donato, P. Ge, J. Oehri, B. Aryal, P. Hao, M. Linnert, N. Burgardt, C. Lücke, M. Weiwad, M. Michel, O. Weiergräber, S. Pollmann, E. Azzarello, S. Mancuso, N. Ferro, Y. Fukao, C. Hoffmann, R. Wedlich Söldner, J. Friml, C. Thomas, M. Geisler, Plant Cell 28 (2016) 930–948.","ama":"Zhu J, Bailly A, Zwiewka M, et al. TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics. <i>Plant Cell</i>. 2016;28(4):930-948. doi:<a href=\"https://doi.org/10.1105/tpc.15.00726\">10.1105/tpc.15.00726</a>"},"isi":1,"oa":1,"oa_version":"Submitted Version","issue":"4","month":"04","title":"TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"publication":"Plant Cell","day":"01","intvolume":"        28","date_published":"2016-04-01T00:00:00Z","publist_id":"6078","external_id":{"isi":["000375416000012"]},"date_updated":"2025-09-22T09:10:52Z","status":"public","page":"930 - 948","year":"2016","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863381/","open_access":"1"}],"publisher":"American Society of Plant Biologists","abstract":[{"lang":"eng","text":"Plant growth and architecture is regulated by the polar distribution of the hormone auxin. Polarity and flexibility of this process is provided by constant cycling of auxin transporter vesicles along actin filaments, coordinated by a positive auxinactin feedback loop. Both polar auxin transport and vesicle cycling are inhibited by synthetic auxin transport inhibitors, such as 1-Nnaphthylphthalamic acid (NPA), counteracting the effect of auxin; however, underlying targets and mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis thaliana ABCB chaperone TWISTED DWARF1 (TWD1).We identify ACTIN7 as a relevant, although likely indirect, TWD1 interactor, and show TWD1-dependent regulation of actin filament organization and dynamics and that TWD1 is required for NPA-mediated actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence of efflux transporters, and as a consequence act7 and twd1 share developmental and physiological phenotypes indicative of defects in auxin transport. These can be phenocopied by NPA treatment or by chemical actin (de)stabilization. We provide evidence that TWD1 determines downstreamlocations of auxin efflux transporters by adjusting actin filament debundling and dynamizing processes and mediating NPA action on the latter. This function appears to be evolutionary conserved since TWD1 expression in budding yeast alters actin polarization and cell polarity and provides NPA sensitivity."}],"author":[{"last_name":"Zhu","full_name":"Zhu, Jinsheng","first_name":"Jinsheng"},{"last_name":"Bailly","full_name":"Bailly, Aurélien","first_name":"Aurélien"},{"last_name":"Zwiewka","first_name":"Marta","full_name":"Zwiewka, Marta"},{"last_name":"Sovero","full_name":"Sovero, Valpuri","first_name":"Valpuri"},{"last_name":"Di Donato","full_name":"Di Donato, Martin","first_name":"Martin"},{"last_name":"Ge","first_name":"Pei","full_name":"Ge, Pei"},{"first_name":"Jacqueline","full_name":"Oehri, Jacqueline","last_name":"Oehri"},{"full_name":"Aryal, Bibek","first_name":"Bibek","last_name":"Aryal"},{"last_name":"Hao","first_name":"Pengchao","full_name":"Hao, Pengchao"},{"last_name":"Linnert","first_name":"Miriam","full_name":"Linnert, Miriam"},{"full_name":"Burgardt, Noelia","first_name":"Noelia","last_name":"Burgardt"},{"last_name":"Lücke","first_name":"Christian","full_name":"Lücke, Christian"},{"first_name":"Matthias","full_name":"Weiwad, Matthias","last_name":"Weiwad"},{"last_name":"Michel","first_name":"Max","full_name":"Michel, Max"},{"last_name":"Weiergräber","full_name":"Weiergräber, Oliver","first_name":"Oliver"},{"full_name":"Pollmann, Stephan","first_name":"Stephan","last_name":"Pollmann"},{"last_name":"Azzarello","first_name":"Elisa","full_name":"Azzarello, Elisa"},{"last_name":"Mancuso","full_name":"Mancuso, Stefano","first_name":"Stefano"},{"last_name":"Ferro","first_name":"Noel","full_name":"Ferro, Noel"},{"full_name":"Fukao, Yoichiro","first_name":"Yoichiro","last_name":"Fukao"},{"full_name":"Hoffmann, Céline","first_name":"Céline","last_name":"Hoffmann"},{"last_name":"Wedlich Söldner","first_name":"Roland","full_name":"Wedlich Söldner, Roland"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí"},{"last_name":"Thomas","full_name":"Thomas, Clément","first_name":"Clément"},{"full_name":"Geisler, Markus","first_name":"Markus","last_name":"Geisler"}],"_id":"1251","acknowledgement":" This work was supported by grants from the European Social Fund (CZ.1.07/2.3.00/20.0043), the Czech Science Foundation GAČR (GA13-40637S) to J.F. and M.Z., the Ministry of Education, Youth, and Sports of the Czech Republic under the project CEITEC 2020 (LQ1601) to M.Z., the Ministry for Higher Education and Research of Luxembourg (REC-LOCM-20140703) to C.T., the Partial Funding Program for Short Stays Abroad of CONICET Argentina (to N.I.B.), Swiss National Funds, the Pool de Recherche of the University of Fribourg, and the Novartis Foundation (all to M.G.). "},{"language":[{"iso":"eng"}],"ec_funded":1,"title":"Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier","month":"07","oa_version":"Submitted Version","issue":"3","oa":1,"project":[{"grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants"}],"isi":1,"citation":{"ama":"Sancho Andrés G, Soriano Ortega E, Gao C, et al. Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier. <i>Plant Physiology</i>. 2016;171(3):1965-1982. doi:<a href=\"https://doi.org/10.1104/pp.16.00373\">10.1104/pp.16.00373</a>","short":"G. Sancho Andrés, E. Soriano Ortega, C. Gao, J. Bernabé Orts, M. Narasimhan, A. Müller, R. Tejos, L. Jiang, J. Friml, F. Aniento, M. Marcote, Plant Physiology 171 (2016) 1965–1982.","ista":"Sancho Andrés G, Soriano Ortega E, Gao C, Bernabé Orts J, Narasimhan M, Müller A, Tejos R, Jiang L, Friml J, Aniento F, Marcote M. 2016. Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier. Plant Physiology. 171(3), 1965–1982.","apa":"Sancho Andrés, G., Soriano Ortega, E., Gao, C., Bernabé Orts, J., Narasimhan, M., Müller, A., … Marcote, M. (2016). Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.16.00373\">https://doi.org/10.1104/pp.16.00373</a>","ieee":"G. Sancho Andrés <i>et al.</i>, “Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier,” <i>Plant Physiology</i>, vol. 171, no. 3. American Society of Plant Biologists, pp. 1965–1982, 2016.","chicago":"Sancho Andrés, Gloria, Esther Soriano Ortega, Caiji Gao, Joan Bernabé Orts, Madhumitha Narasimhan, Anna Müller, Ricardo Tejos, et al. “Sorting Motifs Involved in the Trafficking and Localization of the PIN1 Auxin Efflux Carrier.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2016. <a href=\"https://doi.org/10.1104/pp.16.00373\">https://doi.org/10.1104/pp.16.00373</a>.","mla":"Sancho Andrés, Gloria, et al. “Sorting Motifs Involved in the Trafficking and Localization of the PIN1 Auxin Efflux Carrier.” <i>Plant Physiology</i>, vol. 171, no. 3, American Society of Plant Biologists, 2016, pp. 1965–82, doi:<a href=\"https://doi.org/10.1104/pp.16.00373\">10.1104/pp.16.00373</a>."},"article_processing_charge":"No","quality_controlled":"1","publication_status":"published","type":"journal_article","volume":171,"scopus_import":"1","date_created":"2018-12-11T11:51:01Z","doi":"10.1104/pp.16.00373","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","acknowledgement":"We thank Dr. R. Offringa (Leiden University) for providing the GST-\r\nPIN-CL construct; Sandra Richter and Gerd Jurgens (University of Tübin-\r\ngen) for providing the estradiol-inducible PIN1-RFP construct and the\r\ngnl1 mutant expressing BFA-sensitive GNL1; F.J. Santonja (University of Valencia)\r\nfor help with the statistical analysis; Jurgen Kleine-Vehn, Elke Barbez, and\r\nEva Benkova for helpful discussions; the Salk Institute Genomic Analysis\r\nLaboratory for providing the sequence-indexed Arabidopsis T-DNA in-\r\nsertion mutants; and the greenhouse section and the microscopy section\r\nof SCSIE (University of Valencia) and Pilar Selvi for excellent technical\r\nassistance.","_id":"1264","author":[{"last_name":"Sancho Andrés","first_name":"Gloria","full_name":"Sancho Andrés, Gloria"},{"full_name":"Soriano Ortega, Esther","first_name":"Esther","last_name":"Soriano Ortega"},{"first_name":"Caiji","full_name":"Gao, Caiji","last_name":"Gao"},{"full_name":"Bernabé Orts, Joan","first_name":"Joan","last_name":"Bernabé Orts"},{"orcid":"0000-0002-8600-0671","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","last_name":"Narasimhan","full_name":"Narasimhan, Madhumitha","first_name":"Madhumitha"},{"first_name":"Anna","full_name":"Müller, Anna","id":"420AB15A-F248-11E8-B48F-1D18A9856A87","last_name":"Müller"},{"first_name":"Ricardo","full_name":"Tejos, Ricardo","last_name":"Tejos"},{"last_name":"Jiang","first_name":"Liwen","full_name":"Jiang, Liwen"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","full_name":"Friml, Jirí"},{"first_name":"Fernando","full_name":"Aniento, Fernando","last_name":"Aniento"},{"first_name":"Maria","full_name":"Marcote, Maria","last_name":"Marcote"}],"abstract":[{"lang":"eng","text":"n contrast with the wealth of recent reports about the function of μ-adaptins and clathrin adaptor protein (AP) complexes, there is very little information about the motifs that determine the sorting of membrane proteins within clathrin-coated vesicles in plants. Here, we investigated putative sorting signals in the large cytosolic loop of the Arabidopsis (Arabidopsis thaliana) PIN-FORMED1 (PIN1) auxin transporter, which are involved in binding μ-adaptins and thus in PIN1 trafficking and localization. We found that Phe-165 and Tyr-280, Tyr-328, and Tyr-394 are involved in the binding of different μ-adaptins in vitro. However, only Phe-165, which binds μA(μ2)- and μD(μ3)-adaptin, was found to be essential for PIN1 trafficking and localization in vivo. The PIN1:GFP-F165A mutant showed reduced endocytosis but also localized to intracellular structures containing several layers of membranes and endoplasmic reticulum (ER) markers, suggesting that they correspond to ER or ER-derived membranes. While PIN1:GFP localized normally in a μA (μ2)-adaptin mutant, it accumulated in big intracellular structures containing LysoTracker in a μD (μ3)-adaptin mutant, consistent with previous results obtained with mutants of other subunits of the AP-3 complex. Our data suggest that Phe-165, through the binding of μA (μ2)- and μD (μ3)-adaptin, is important for PIN1 endocytosis and for PIN1 trafficking along the secretory pathway, respectively."}],"publisher":"American Society of Plant Biologists","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4936568/"}],"page":"1965 - 1982","year":"2016","status":"public","date_updated":"2025-09-22T08:58:52Z","external_id":{"isi":["000381303300037"]},"publist_id":"6059","date_published":"2016-07-01T00:00:00Z","intvolume":"       171","day":"01","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"publication":"Plant Physiology"},{"publication":"Scientific Reports","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"date_updated":"2025-09-22T08:43:50Z","external_id":{"pmid":["27649687"],"isi":["000383572400003"]},"status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"ddc":["581"],"day":"21","intvolume":"         6","publist_id":"6042","date_published":"2016-09-21T00:00:00Z","publisher":"Nature Publishing Group","author":[{"last_name":"Mazur","full_name":"Mazur, Ewa","first_name":"Ewa"},{"first_name":"Eva","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","last_name":"Benková"},{"first_name":"Jirí","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml"}],"abstract":[{"lang":"eng","text":"Synchronized tissue polarization during regeneration or de novo vascular tissue formation is a plant-specific example of intercellular communication and coordinated development. According to the canalization hypothesis, the plant hormone auxin serves as polarizing signal that mediates directional channel formation underlying the spatio-temporal vasculature patterning. A necessary part of canalization is a positive feedback between auxin signaling and polarity of the intercellular auxin flow. The cellular and molecular mechanisms of this process are still poorly understood, not the least, because of a lack of a suitable model system. We show that the main genetic model plant, Arabidopsis (Arabidopsis thaliana) can be used to study the canalization during vascular cambium regeneration and new vasculature formation. We monitored localized auxin responses, directional auxin-transport channels formation, and establishment of new vascular cambium polarity during regenerative processes after stem wounding. The increased auxin response above and around the wound preceded the formation of PIN1 auxin transporter-marked channels from the primarily homogenous tissue and the transient, gradual changes in PIN1 localization preceded the polarity of newly formed vascular tissue. Thus, Arabidopsis is a useful model for studies of coordinated tissue polarization and vasculature formation after wounding allowing for genetic and mechanistic dissection of the canalization hypothesis."}],"year":"2016","file_date_updated":"2020-07-14T12:44:42Z","pubrep_id":"692","file":[{"file_id":"5008","date_created":"2018-12-12T10:13:25Z","creator":"system","checksum":"ee371fbc9124ad93157a95829264e4fe","file_size":2895147,"date_updated":"2020-07-14T12:44:42Z","content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_name":"IST-2016-692-v1+1_srep33754.pdf"}],"acknowledgement":"We wish to thank Prof. Ewa U. Kurczyńska for initiation of this work and valuable advices. We thank Martine De Cock for help in preparing the manuscript. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP), the European Social Fund (CZ.1.07/2.3.00/20.0043), and the Czech Science Foundation GAČR (GA13-40637 S) to J.F., (GA 13-39982S) to E.B. and E.M. and in part by the European Regional Development Fund (project “CEITEC, Central European Institute of Technology”, CZ.1.05/1.1.00/02.0068).","article_number":"33754","_id":"1274","pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","volume":6,"scopus_import":"1","type":"journal_article","doi":"10.1038/srep33754","date_created":"2018-12-11T11:51:05Z","quality_controlled":"1","article_processing_charge":"No","isi":1,"citation":{"ama":"Mazur E, Benková E, Friml J. Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. <i>Scientific Reports</i>. 2016;6. doi:<a href=\"https://doi.org/10.1038/srep33754\">10.1038/srep33754</a>","short":"E. Mazur, E. Benková, J. Friml, Scientific Reports 6 (2016).","ieee":"E. Mazur, E. Benková, and J. Friml, “Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis,” <i>Scientific Reports</i>, vol. 6. Nature Publishing Group, 2016.","apa":"Mazur, E., Benková, E., &#38; Friml, J. (2016). Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/srep33754\">https://doi.org/10.1038/srep33754</a>","ista":"Mazur E, Benková E, Friml J. 2016. Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. Scientific Reports. 6, 33754.","chicago":"Mazur, Ewa, Eva Benková, and Jiří Friml. “Vascular Cambium Regeneration and Vessel Formation in Wounded Inflorescence Stems of Arabidopsis.” <i>Scientific Reports</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/srep33754\">https://doi.org/10.1038/srep33754</a>.","mla":"Mazur, Ewa, et al. “Vascular Cambium Regeneration and Vessel Formation in Wounded Inflorescence Stems of Arabidopsis.” <i>Scientific Reports</i>, vol. 6, 33754, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/srep33754\">10.1038/srep33754</a>."},"publication_status":"published","title":"Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis","month":"09","language":[{"iso":"eng"}],"related_material":{"record":[{"status":"public","id":"545","relation":"later_version"}]},"has_accepted_license":"1","oa_version":"Published Version","oa":1},{"issue":"39","oa_version":"Preprint","oa":1,"language":[{"iso":"eng"}],"title":"Danger-associated peptide signaling in Arabidopsis requires clathrin","month":"09","publication_status":"published","citation":{"ista":"Ortiz Morea F, Savatin D, Dejonghe W, Kumar R, Luo Y, Adamowski M, Van Begin J, Dressano K, De Oliveira G, Zhao X, Lu Q, Madder A, Friml J, De Moura D, Russinova E. 2016. Danger-associated peptide signaling in Arabidopsis requires clathrin. PNAS. 113(39), 11028–11033.","apa":"Ortiz Morea, F., Savatin, D., Dejonghe, W., Kumar, R., Luo, Y., Adamowski, M., … Russinova, E. (2016). Danger-associated peptide signaling in Arabidopsis requires clathrin. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1605588113\">https://doi.org/10.1073/pnas.1605588113</a>","ieee":"F. Ortiz Morea <i>et al.</i>, “Danger-associated peptide signaling in Arabidopsis requires clathrin,” <i>PNAS</i>, vol. 113, no. 39. National Academy of Sciences, pp. 11028–11033, 2016.","mla":"Ortiz Morea, Fausto, et al. “Danger-Associated Peptide Signaling in Arabidopsis Requires Clathrin.” <i>PNAS</i>, vol. 113, no. 39, National Academy of Sciences, 2016, pp. 11028–33, doi:<a href=\"https://doi.org/10.1073/pnas.1605588113\">10.1073/pnas.1605588113</a>.","chicago":"Ortiz Morea, Fausto, Daniel Savatin, Wim Dejonghe, Rahul Kumar, Yu Luo, Maciek Adamowski, Jos Van Begin, et al. “Danger-Associated Peptide Signaling in Arabidopsis Requires Clathrin.” <i>PNAS</i>. National Academy of Sciences, 2016. <a href=\"https://doi.org/10.1073/pnas.1605588113\">https://doi.org/10.1073/pnas.1605588113</a>.","ama":"Ortiz Morea F, Savatin D, Dejonghe W, et al. Danger-associated peptide signaling in Arabidopsis requires clathrin. <i>PNAS</i>. 2016;113(39):11028-11033. doi:<a href=\"https://doi.org/10.1073/pnas.1605588113\">10.1073/pnas.1605588113</a>","short":"F. Ortiz Morea, D. Savatin, W. Dejonghe, R. Kumar, Y. Luo, M. Adamowski, J. Van Begin, K. Dressano, G. De Oliveira, X. Zhao, Q. Lu, A. Madder, J. Friml, D. De Moura, E. Russinova, PNAS 113 (2016) 11028–11033."},"isi":1,"quality_controlled":"1","article_processing_charge":"No","date_created":"2018-12-11T11:51:06Z","doi":"10.1073/pnas.1605588113","type":"journal_article","volume":113,"scopus_import":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"1277","acknowledgement":"F.A.O.-M. was supported by special\r\nresearch funding from the Flemish Government for a joint doctorate fellowship\r\nat Ghent University, and funding from the Student Program\r\n–\r\nGraduate Studies\r\nPlan Program from the Coordination for the Improvement of Higher Educa-\r\ntion Personnel, Brazil, for a doctorate fellowship at the University of São Paulo.\r\nX.Z. and Q.L. are indebted to the China Science Council and G.P.d.O. to the\r\n“\r\nCiência sem Fronteiras\r\n”\r\nfor predoctoral fellowships. R.K. and Y.L. have re-\r\nceived postdoctoral fellowships from the Belgian Science Policy Office. This\r\nresearch was supported by Flanders Research Foundation Grant G008416N\r\n(to E.R.) and by the São Paulo Research Foundation and the National Council\r\nfor Scientific and Technological Development (CNPq) (D.S.d.M.). D.S.d.M. is a\r\nresearch fellow of CNPq.\r\nWe thank D. Van Damme, E. Mylle, M. Castro Silva-Filho,\r\nand J. Goeman for providing usefu\r\nl advice and technical assistance;\r\nI. Hara-Nishimura, J. Lin, G. Jürgens, M. A. Johnson, and P. Bozhkov for sharing\r\npublished materials; and M. Nowack and M. Fendrych for kindly donating the\r\npUBQ10::ATG8-YFP\r\n-expressing marker line.","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047203/"}],"year":"2016","page":"11028 - 11033","author":[{"first_name":"Fausto","full_name":"Ortiz Morea, Fausto","last_name":"Ortiz Morea"},{"full_name":"Savatin, Daniel","first_name":"Daniel","last_name":"Savatin"},{"last_name":"Dejonghe","full_name":"Dejonghe, Wim","first_name":"Wim"},{"last_name":"Kumar","first_name":"Rahul","full_name":"Kumar, Rahul"},{"last_name":"Luo","first_name":"Yu","full_name":"Luo, Yu"},{"full_name":"Adamowski, Maciek","first_name":"Maciek","last_name":"Adamowski","orcid":"0000-0001-6463-5257","id":"45F536D2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Van Begin, Jos","first_name":"Jos","last_name":"Van Begin"},{"first_name":"Keini","full_name":"Dressano, Keini","last_name":"Dressano"},{"last_name":"De Oliveira","full_name":"De Oliveira, Guilherme","first_name":"Guilherme"},{"first_name":"Xiuyang","full_name":"Zhao, Xiuyang","last_name":"Zhao"},{"last_name":"Lu","first_name":"Qing","full_name":"Lu, Qing"},{"first_name":"Annemieke","full_name":"Madder, Annemieke","last_name":"Madder"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"De Moura","first_name":"Daniel","full_name":"De Moura, Daniel"},{"full_name":"Russinova, Eugenia","first_name":"Eugenia","last_name":"Russinova"}],"abstract":[{"text":"The Arabidopsis thaliana endogenous elicitor peptides (AtPeps) are released into the apoplast after cellular damage caused by pathogens or wounding to induce innate immunity by direct binding to the membrane-localized leucine-rich repeat receptor kinases, PEP RECEPTOR1 (PEPR1) and PEPR2. Although the PEPR-mediated signaling components and responses have been studied extensively, the contributions of the subcellular localization and dynamics of the active PEPRs remain largely unknown. We used live-cell imaging of the fluorescently labeled and bioactive pep1 to visualize the intracellular behavior of the PEPRs in the Arabidopsis root meristem. We found that AtPep1 decorated the plasma membrane (PM) in a receptor-dependent manner and cointernalized with PEPRs. Trafficking of the AtPep1-PEPR1 complexes to the vacuole required neither the trans-Golgi network/early endosome (TGN/EE)-localized vacuolar H+ -ATPase activity nor the function of the brefeldin A-sensitive ADP-ribosylation factor-guanine exchange factors (ARF-GEFs). In addition, AtPep1 and different TGN/EE markers colocalized only rarely, implying that the intracellular route of this receptor-ligand pair is largely independent of the TGN/EE. Inducible overexpression of the Arabidopsis clathrin coat disassembly factor, Auxilin2, which inhibits clathrin-mediated endocytosis (CME), impaired the AtPep1-PEPR1 internalization and compromised AtPep1-mediated responses. Our results show that clathrin function at the PM is required to induce plant defense responses, likely through CME of cell surface-located signaling components.\r\n","lang":"eng"}],"publisher":"National Academy of Sciences","publist_id":"6039","date_published":"2016-09-27T00:00:00Z","day":"27","intvolume":"       113","status":"public","external_id":{"isi":["000383954700066"]},"date_updated":"2025-09-22T08:37:42Z","publication":"PNAS","department":[{"_id":"JiFr"}]},{"file_date_updated":"2020-07-14T12:44:45Z","year":"2016","author":[{"full_name":"Fendrych, Matyas","first_name":"Matyas","last_name":"Fendrych","orcid":"0000-0002-9767-8699","id":"43905548-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Leung, Jeffrey","first_name":"Jeffrey","last_name":"Leung"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí"}],"abstract":[{"text":"Despite being composed of immobile cells, plants reorient along directional stimuli. The hormone auxin is redistributed in stimulated organs leading to differential growth and bending. Auxin application triggers rapid cell wall acidification and elongation of aerial organs of plants, but the molecular players mediating these effects are still controversial. Here we use genetically-encoded pH and auxin signaling sensors, pharmacological and genetic manipulations available for Arabidopsis etiolated hypocotyls to clarify how auxin is perceived and the downstream growth executed. We show that auxin-induced acidification occurs by local activation of H+-ATPases, which in the context of gravity response is restricted to the lower organ side. This auxin-stimulated acidification and growth require TIR1/AFB-Aux/IAA nuclear auxin perception. In addition, auxin-induced gene transcription and specifically SAUR proteins are crucial downstream mediators of this growth. Our study provides strong experimental support for the acid growth theory and clarified the contribution of the upstream auxin perception mechanisms.","lang":"eng"}],"publisher":"eLife Sciences Publications","_id":"1344","article_number":"e19048","pubrep_id":"654","acknowledgement":"The authors express their gratitude to Veronika Bierbaum, Robert Hauschild for help with MATLAB,\r\nDaniel von Wangenheim for the gravitropism assay. We are thankful to Bill Gray, Mark Estelle,\r\nMichael Prigge, Ottoline Leyser, Claudia Oecking for sharing the seeds with us. We thank Katelyn\r\nSageman-Furnas and the members of the Friml lab for critical reading of the manuscript. The\r\nresearch leading to these results has received funding from the People Programme (Marie Curie\r\nActions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant\r\nagreement n° 291734. This work was also supported by the European Research Council (project\r\nERC-2011-StG-20101109-PSDP).","file":[{"relation":"main_file","file_name":"IST-2016-693-v1+1_e19048-download.pdf","creator":"system","date_created":"2018-12-12T10:09:24Z","checksum":"9209541fbba00f24daad21a5d568540d","file_id":"4748","content_type":"application/pdf","access_level":"open_access","date_updated":"2020-07-14T12:44:45Z","file_size":5666343}],"corr_author":"1","publication":"eLife","department":[{"_id":"JiFr"}],"publist_id":"5908","date_published":"2016-09-14T00:00:00Z","intvolume":"         5","day":"14","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"ddc":["581"],"date_updated":"2025-09-22T08:16:03Z","external_id":{"isi":["000385559100001"]},"publication_status":"published","project":[{"grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"},{"grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants"}],"isi":1,"citation":{"ieee":"M. Fendrych, J. Leung, and J. Friml, “TIR1 AFB Aux IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls,” <i>eLife</i>, vol. 5. eLife Sciences Publications, 2016.","apa":"Fendrych, M., Leung, J., &#38; Friml, J. (2016). TIR1 AFB Aux IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.19048\">https://doi.org/10.7554/eLife.19048</a>","ista":"Fendrych M, Leung J, Friml J. 2016. TIR1 AFB Aux IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. eLife. 5, e19048.","chicago":"Fendrych, Matyas, Jeffrey Leung, and Jiří Friml. “TIR1 AFB Aux IAA Auxin Perception Mediates Rapid Cell Wall Acidification and Growth of Arabidopsis Hypocotyls.” <i>ELife</i>. eLife Sciences Publications, 2016. <a href=\"https://doi.org/10.7554/eLife.19048\">https://doi.org/10.7554/eLife.19048</a>.","mla":"Fendrych, Matyas, et al. “TIR1 AFB Aux IAA Auxin Perception Mediates Rapid Cell Wall Acidification and Growth of Arabidopsis Hypocotyls.” <i>ELife</i>, vol. 5, e19048, eLife Sciences Publications, 2016, doi:<a href=\"https://doi.org/10.7554/eLife.19048\">10.7554/eLife.19048</a>.","ama":"Fendrych M, Leung J, Friml J. TIR1 AFB Aux IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. <i>eLife</i>. 2016;5. doi:<a href=\"https://doi.org/10.7554/eLife.19048\">10.7554/eLife.19048</a>","short":"M. Fendrych, J. Leung, J. Friml, ELife 5 (2016)."},"article_processing_charge":"No","quality_controlled":"1","oa_version":"Published Version","oa":1,"has_accepted_license":"1","language":[{"iso":"eng"}],"month":"09","ec_funded":1,"title":"TIR1 AFB Aux IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:51:29Z","doi":"10.7554/eLife.19048","type":"journal_article","volume":5,"scopus_import":"1"},{"oa_version":"Published Version","oa":1,"has_accepted_license":"1","language":[{"iso":"eng"}],"title":"Plasma membrane: Negative attraction","month":"07","publication_status":"published","isi":1,"citation":{"ista":"Molnar G, Fendrych M, Friml J. 2016. Plasma membrane: Negative attraction. Nature Plants. 2, 16102.","apa":"Molnar, G., Fendrych, M., &#38; Friml, J. (2016). Plasma membrane: Negative attraction. <i>Nature Plants</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nplants.2016.102\">https://doi.org/10.1038/nplants.2016.102</a>","ieee":"G. Molnar, M. Fendrych, and J. Friml, “Plasma membrane: Negative attraction,” <i>Nature Plants</i>, vol. 2. Nature Publishing Group, 2016.","mla":"Molnar, Gergely, et al. “Plasma Membrane: Negative Attraction.” <i>Nature Plants</i>, vol. 2, 16102, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/nplants.2016.102\">10.1038/nplants.2016.102</a>.","chicago":"Molnar, Gergely, Matyas Fendrych, and Jiří Friml. “Plasma Membrane: Negative Attraction.” <i>Nature Plants</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/nplants.2016.102\">https://doi.org/10.1038/nplants.2016.102</a>.","ama":"Molnar G, Fendrych M, Friml J. Plasma membrane: Negative attraction. <i>Nature Plants</i>. 2016;2. doi:<a href=\"https://doi.org/10.1038/nplants.2016.102\">10.1038/nplants.2016.102</a>","short":"G. Molnar, M. Fendrych, J. Friml, Nature Plants 2 (2016)."},"quality_controlled":"1","article_processing_charge":"No","date_created":"2018-12-11T11:51:30Z","doi":"10.1038/nplants.2016.102","type":"journal_article","volume":2,"scopus_import":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"1345","article_number":"16102","pubrep_id":"1007","file":[{"file_name":"IST-2018-1007-v1+1_Molnar_NatPlants_2016.pdf","relation":"main_file","file_size":127781,"date_updated":"2020-07-14T12:44:45Z","access_level":"open_access","content_type":"application/pdf","file_id":"4954","checksum":"9ba65f558563b287f875f48fa9f30fb2","creator":"system","date_created":"2018-12-12T10:12:36Z"},{"relation":"main_file","file_name":"IST-2018-1007-v1+2_Molnar_NatPlants_2016_editor_statement.pdf","checksum":"550d252be808d8ca2b43e83dddb4212f","date_created":"2018-12-12T10:12:37Z","creator":"system","file_id":"4955","access_level":"open_access","content_type":"application/pdf","file_size":430556,"date_updated":"2020-07-14T12:44:45Z"}],"corr_author":"1","file_date_updated":"2020-07-14T12:44:45Z","year":"2016","author":[{"last_name":"Molnar","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Gergely","full_name":"Molnar, Gergely"},{"last_name":"Fendrych","orcid":"0000-0002-9767-8699","id":"43905548-F248-11E8-B48F-1D18A9856A87","full_name":"Fendrych, Matyas","first_name":"Matyas"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí"}],"abstract":[{"text":"The electrostatic charge at the inner surface of the plasma membrane is strongly negative in higher organisms. A new study shows that phosphatidylinositol-4-phosphate plays a critical role in establishing plasma membrane surface charge in Arabidopsis, which regulates the correct localization of signalling components.","lang":"eng"}],"publisher":"Nature Publishing Group","publist_id":"5907","date_published":"2016-07-01T00:00:00Z","intvolume":"         2","day":"01","status":"public","ddc":["581"],"date_updated":"2025-09-22T08:15:28Z","external_id":{"isi":["000380346500013"]},"publication":"Nature Plants","department":[{"_id":"JiFr"}]},{"date_created":"2018-12-11T11:51:30Z","doi":"10.1038/ncomms11710","type":"journal_article","volume":7,"scopus_import":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa_version":"Published Version","oa":1,"related_material":{"record":[{"relation":"dissertation_contains","id":"7172","status":"public"}]},"has_accepted_license":"1","language":[{"iso":"eng"}],"month":"06","ec_funded":1,"title":"Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification","publication_status":"published","project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300"}],"citation":{"short":"W. Dejonghe, S. Kuenen, E. Mylle, M.K. Vasileva, O. Keech, C. Viotti, J. Swerts, M. Fendrych, F. Ortiz Morea, K. Mishev, S. Delang, S. Scholl, X. Zarza, M. Heilmann, J. Kourelis, J. Kasprowicz, L. Nguyen, A. Drozdzecki, I. Van Houtte, A. Szatmári, M. Majda, G. Baisa, S. Bednarek, S. Robert, D. Audenaert, C. Testerink, T. Munnik, D. Van Damme, I. Heilmann, K. Schumacher, J. Winne, J. Friml, P. Verstreken, E. Russinova, Nature Communications 7 (2016).","ama":"Dejonghe W, Kuenen S, Mylle E, et al. Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification. <i>Nature Communications</i>. 2016;7. doi:<a href=\"https://doi.org/10.1038/ncomms11710\">10.1038/ncomms11710</a>","mla":"Dejonghe, Wim, et al. “Mitochondrial Uncouplers Inhibit Clathrin-Mediated Endocytosis Largely through Cytoplasmic Acidification.” <i>Nature Communications</i>, vol. 7, 11710, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/ncomms11710\">10.1038/ncomms11710</a>.","chicago":"Dejonghe, Wim, Sabine Kuenen, Evelien Mylle, Mina K Vasileva, Olivier Keech, Corrado Viotti, Jef Swerts, et al. “Mitochondrial Uncouplers Inhibit Clathrin-Mediated Endocytosis Largely through Cytoplasmic Acidification.” <i>Nature Communications</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/ncomms11710\">https://doi.org/10.1038/ncomms11710</a>.","apa":"Dejonghe, W., Kuenen, S., Mylle, E., Vasileva, M. K., Keech, O., Viotti, C., … Russinova, E. (2016). Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification. <i>Nature Communications</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncomms11710\">https://doi.org/10.1038/ncomms11710</a>","ieee":"W. Dejonghe <i>et al.</i>, “Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification,” <i>Nature Communications</i>, vol. 7. Nature Publishing Group, 2016.","ista":"Dejonghe W, Kuenen S, Mylle E, Vasileva MK, Keech O, Viotti C, Swerts J, Fendrych M, Ortiz Morea F, Mishev K, Delang S, Scholl S, Zarza X, Heilmann M, Kourelis J, Kasprowicz J, Nguyen L, Drozdzecki A, Van Houtte I, Szatmári A, Majda M, Baisa G, Bednarek S, Robert S, Audenaert D, Testerink C, Munnik T, Van Damme D, Heilmann I, Schumacher K, Winne J, Friml J, Verstreken P, Russinova E. 2016. Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification. Nature Communications. 7, 11710."},"isi":1,"article_processing_charge":"No","quality_controlled":"1","publist_id":"5906","date_published":"2016-06-08T00:00:00Z","day":"08","intvolume":"         7","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"status":"public","ddc":["570"],"date_updated":"2026-04-08T13:54:44Z","external_id":{"isi":["000377899800001"]},"publication":"Nature Communications","department":[{"_id":"JiFr"}],"_id":"1346","article_number":"11710","pubrep_id":"653","file":[{"relation":"main_file","file_name":"IST-2016-653-v1+1_ncomms11710_1_.pdf","file_id":"5369","checksum":"e8dc81b3e44db5a7718d7f1501ce1aa7","creator":"system","date_created":"2018-12-12T10:18:47Z","file_size":3532505,"date_updated":"2020-07-14T12:44:45Z","access_level":"open_access","content_type":"application/pdf"}],"acknowledgement":"We thank Yvon Jaillais, Ikuko Hara-Nishimura, Akihiko Nakano, Takashi Ueda and Jinxing Lin for providing materials, Natasha Raikhel, Glenn Hicks, Steffen Vanneste, and Ricardo Tejos for useful suggestions, Patrick Callaerts for providing S2 Drosophila cell cultures, Michael Sixt for providing HeLa cells, Annick Bleys for literature searches, VIB Bio Imaging Core for help with imaging conditions and Martine De Cock for help in preparing the article. This work was supported by the Agency for Innovation by Science\r\nand Technology for a pre-doctoral fellowship to W.D.; the Research fund KU Leuven\r\n(GOA), a Methusalem grant of the Flemish government and VIB to S.K., J.K. and P.V.;\r\nby the Netherlands Organisation for Scientific Research (NWO) for ALW grants\r\n846.11.002 (C.T.) and 867.15.020 (T.M.); the European Research Council (project\r\nERC-2011-StG-20101109 PSDP) (to J.F.); a European Research Council (ERC) Starting\r\nGrant (grant 260678) (to P.V.), the Research Foundation-Flanders (grants G.0747.09,\r\nG094011 and G095511) (to P.V.), the Hercules Foundation, an Interuniversity Attraction\r\nPoles Poles Program, initiated by the Belgian State, Science Policy Office (to P.V.),\r\nthe Swedish VetenskapsRådet grant to O.K., the Ghent University ‘Bijzonder\r\nOnderzoek Fonds’ (BOF) for a predoctoral fellowship to F.A.O.-M., the Research\r\nFoundation-Flanders (FWO) to K.M. and E.R.","file_date_updated":"2020-07-14T12:44:45Z","year":"2016","author":[{"last_name":"Dejonghe","first_name":"Wim","full_name":"Dejonghe, Wim"},{"last_name":"Kuenen","full_name":"Kuenen, Sabine","first_name":"Sabine"},{"full_name":"Mylle, Evelien","first_name":"Evelien","last_name":"Mylle"},{"id":"3407EB18-F248-11E8-B48F-1D18A9856A87","last_name":"Vasileva","first_name":"Mina K","full_name":"Vasileva, Mina K"},{"last_name":"Keech","full_name":"Keech, Olivier","first_name":"Olivier"},{"last_name":"Viotti","full_name":"Viotti, Corrado","first_name":"Corrado"},{"last_name":"Swerts","full_name":"Swerts, Jef","first_name":"Jef"},{"last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9767-8699","first_name":"Matyas","full_name":"Fendrych, Matyas"},{"first_name":"Fausto","full_name":"Ortiz Morea, Fausto","last_name":"Ortiz Morea"},{"first_name":"Kiril","full_name":"Mishev, Kiril","last_name":"Mishev"},{"full_name":"Delang, Simon","first_name":"Simon","last_name":"Delang"},{"last_name":"Scholl","first_name":"Stefan","full_name":"Scholl, Stefan"},{"last_name":"Zarza","first_name":"Xavier","full_name":"Zarza, Xavier"},{"last_name":"Heilmann","full_name":"Heilmann, Mareike","first_name":"Mareike"},{"full_name":"Kourelis, Jiorgos","first_name":"Jiorgos","last_name":"Kourelis"},{"full_name":"Kasprowicz, Jaroslaw","first_name":"Jaroslaw","last_name":"Kasprowicz"},{"last_name":"Nguyen","first_name":"Le","full_name":"Nguyen, Le"},{"last_name":"Drozdzecki","first_name":"Andrzej","full_name":"Drozdzecki, Andrzej"},{"first_name":"Isabelle","full_name":"Van Houtte, Isabelle","last_name":"Van Houtte"},{"last_name":"Szatmári","first_name":"Anna","full_name":"Szatmári, Anna"},{"full_name":"Majda, Mateusz","first_name":"Mateusz","last_name":"Majda"},{"last_name":"Baisa","full_name":"Baisa, Gary","first_name":"Gary"},{"full_name":"Bednarek, Sebastian","first_name":"Sebastian","last_name":"Bednarek"},{"full_name":"Robert, Stéphanie","first_name":"Stéphanie","last_name":"Robert"},{"full_name":"Audenaert, Dominique","first_name":"Dominique","last_name":"Audenaert"},{"full_name":"Testerink, Christa","first_name":"Christa","last_name":"Testerink"},{"last_name":"Munnik","full_name":"Munnik, Teun","first_name":"Teun"},{"first_name":"Daniël","full_name":"Van Damme, Daniël","last_name":"Van Damme"},{"last_name":"Heilmann","first_name":"Ingo","full_name":"Heilmann, Ingo"},{"last_name":"Schumacher","full_name":"Schumacher, Karin","first_name":"Karin"},{"last_name":"Winne","full_name":"Winne, Johan","first_name":"Johan"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí"},{"last_name":"Verstreken","full_name":"Verstreken, Patrik","first_name":"Patrik"},{"last_name":"Russinova","full_name":"Russinova, Eugenia","first_name":"Eugenia"}],"abstract":[{"text":"ATP production requires the establishment of an electrochemical proton gradient across the inner mitochondrial membrane. Mitochondrial uncouplers dissipate this proton gradient and disrupt numerous cellular processes, including vesicular trafficking, mainly through energy depletion. Here we show that Endosidin9 (ES9), a novel mitochondrial uncoupler, is a potent inhibitor of clathrin-mediated endocytosis (CME) in different systems and that ES9 induces inhibition of CME not because of its effect on cellular ATP, but rather due to its protonophore activity that leads to cytoplasm acidification. We show that the known tyrosine kinase inhibitor tyrphostinA23, which is routinely used to block CME, displays similar properties, thus questioning its use as a specific inhibitor of cargo recognition by the AP-2 adaptor complex via tyrosine motif-based endocytosis signals. Furthermore, we show that cytoplasm acidification dramatically affects the dynamics and recruitment of clathrin and associated adaptors, and leads to reduction of phosphatidylinositol 4,5-biphosphate from the plasma membrane.","lang":"eng"}],"publisher":"Nature Publishing Group"},{"language":[{"iso":"eng"}],"month":"10","title":"Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis","issue":"2","oa_version":"Submitted Version","oa":1,"has_accepted_license":"1","citation":{"chicago":"Pernisová, Markéta, Tomas Prat, Peter Grones, Danka Haruštiaková, Martina Matonohova, Lukáš Spíchal, Tomasz Nodzyński, Jiří Friml, and Jan Hejátko. “Cytokinins Influence Root Gravitropism via Differential Regulation of Auxin Transporter Expression and Localization in Arabidopsis.” <i>New Phytologist</i>. Wiley-Blackwell, 2016. <a href=\"https://doi.org/10.1111/nph.14049\">https://doi.org/10.1111/nph.14049</a>.","mla":"Pernisová, Markéta, et al. “Cytokinins Influence Root Gravitropism via Differential Regulation of Auxin Transporter Expression and Localization in Arabidopsis.” <i>New Phytologist</i>, vol. 212, no. 2, Wiley-Blackwell, 2016, pp. 497–509, doi:<a href=\"https://doi.org/10.1111/nph.14049\">10.1111/nph.14049</a>.","ieee":"M. Pernisová <i>et al.</i>, “Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis,” <i>New Phytologist</i>, vol. 212, no. 2. Wiley-Blackwell, pp. 497–509, 2016.","apa":"Pernisová, M., Prat, T., Grones, P., Haruštiaková, D., Matonohova, M., Spíchal, L., … Hejátko, J. (2016). Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis. <i>New Phytologist</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/nph.14049\">https://doi.org/10.1111/nph.14049</a>","ista":"Pernisová M, Prat T, Grones P, Haruštiaková D, Matonohova M, Spíchal L, Nodzyński T, Friml J, Hejátko J. 2016. Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis. New Phytologist. 212(2), 497–509.","short":"M. Pernisová, T. Prat, P. Grones, D. Haruštiaková, M. Matonohova, L. Spíchal, T. Nodzyński, J. Friml, J. Hejátko, New Phytologist 212 (2016) 497–509.","ama":"Pernisová M, Prat T, Grones P, et al. Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis. <i>New Phytologist</i>. 2016;212(2):497-509. doi:<a href=\"https://doi.org/10.1111/nph.14049\">10.1111/nph.14049</a>"},"isi":1,"quality_controlled":"1","article_processing_charge":"No","publication_status":"published","type":"journal_article","volume":212,"scopus_import":"1","date_created":"2018-12-11T11:51:38Z","doi":"10.1111/nph.14049","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","pubrep_id":"1006","acknowledgement":"Funded by Ministry of Education, Youth and Sports Czech Republic. Grant Numbers: CEITEC 2020, LQ1601, LO1204, LH14104 and The European Research Council. Grant Number: ERC-2011-StG-20101109-PSDP and The Czech Science Foundation. Grant Numbers: GAP501/11/1150, GA13-40637S, GP14-30004P","file":[{"file_id":"5108","checksum":"27fd841ceaf0403559d7048ef51500f9","date_created":"2018-12-12T10:14:53Z","creator":"system","date_updated":"2020-07-14T12:44:47Z","file_size":972763,"access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_name":"IST-2018-1006-v1+1_Pernisova_NewPhytol_2016_peer_review.pdf"}],"_id":"1372","author":[{"full_name":"Pernisová, Markéta","first_name":"Markéta","last_name":"Pernisová"},{"first_name":"Tomas","full_name":"Prat, Tomas","id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87","last_name":"Prat"},{"first_name":"Peter","full_name":"Grones, Peter","last_name":"Grones","id":"399876EC-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Haruštiaková","full_name":"Haruštiaková, Danka","first_name":"Danka"},{"first_name":"Martina","full_name":"Matonohova, Martina","last_name":"Matonohova"},{"last_name":"Spíchal","full_name":"Spíchal, Lukáš","first_name":"Lukáš"},{"last_name":"Nodzyński","full_name":"Nodzyński, Tomasz","first_name":"Tomasz"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","first_name":"Jirí"},{"last_name":"Hejátko","first_name":"Jan","full_name":"Hejátko, Jan"}],"abstract":[{"text":"Redirection of intercellular auxin fluxes via relocalization of the PIN-FORMED 3 (PIN3) and PIN7 auxin efflux carriers has been suggested to be necessary for the root gravitropic response. Cytokinins have also been proposed to play a role in controlling root gravitropism, but conclusive evidence is lacking. We present a detailed study of the dynamics of root bending early after gravistimulation, which revealed a delayed gravitropic response in transgenic lines with depleted endogenous cytokinins (Pro35S:AtCKX) and cytokinin signaling mutants. Pro35S:AtCKX lines, as well as a cytokinin receptor mutant ahk3, showed aberrations in the auxin response distribution in columella cells consistent with defects in the auxin transport machinery. Using in vivo real-time imaging of PIN3-GFP and PIN7-GFP in AtCKX3 overexpression and ahk3 backgrounds, we observed wild-type-like relocalization of PIN proteins in the columella early after gravistimulation, with gravity-induced relocalization of PIN7 faster than that of PIN3. Nonetheless, the cellular distribution of PIN3 and PIN7 and expression of PIN7 and the auxin influx carrier AUX1 was affected in AtCKX overexpression lines. Based on the retained cytokinin sensitivity in pin3 pin4 pin7 mutant, we propose the AUX1-mediated auxin transport rather than columella-located PIN proteins as a target of endogenous cytokinins in the control of root gravitropism.","lang":"eng"}],"publisher":"Wiley-Blackwell","file_date_updated":"2020-07-14T12:44:47Z","year":"2016","page":"497 - 509","status":"public","ddc":["581"],"date_updated":"2025-09-22T07:36:11Z","external_id":{"isi":["000383595700023"]},"publist_id":"5839","date_published":"2016-10-01T00:00:00Z","day":"01","intvolume":"       212","publication":"New Phytologist","department":[{"_id":"JiFr"}]},{"doi":"10.1016/j.plantsci.2016.05.014","date_created":"2018-12-11T11:51:51Z","volume":250,"scopus_import":"1","type":"journal_article","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","pmid":1,"has_accepted_license":"1","oa_version":"Submitted Version","oa":1,"title":"Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana pollen development","month":"09","language":[{"iso":"eng"}],"publication_status":"published","article_processing_charge":"No","quality_controlled":"1","isi":1,"citation":{"ama":"Ugalde J, Rodríguez Furlán C, De Rycke R, et al. Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana pollen development. <i>Plant Science</i>. 2016;250:10-19. doi:<a href=\"https://doi.org/10.1016/j.plantsci.2016.05.014\">10.1016/j.plantsci.2016.05.014</a>","short":"J. Ugalde, C. Rodríguez Furlán, R. De Rycke, L. Norambuena, J. Friml, G. León, R. Tejos, Plant Science 250 (2016) 10–19.","ista":"Ugalde J, Rodríguez Furlán C, De Rycke R, Norambuena L, Friml J, León G, Tejos R. 2016. Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana pollen development. Plant Science. 250, 10–19.","apa":"Ugalde, J., Rodríguez Furlán, C., De Rycke, R., Norambuena, L., Friml, J., León, G., &#38; Tejos, R. (2016). Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana pollen development. <i>Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.plantsci.2016.05.014\">https://doi.org/10.1016/j.plantsci.2016.05.014</a>","ieee":"J. Ugalde <i>et al.</i>, “Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana pollen development,” <i>Plant Science</i>, vol. 250. Elsevier, pp. 10–19, 2016.","chicago":"Ugalde, José, Cecilia Rodríguez Furlán, Riet De Rycke, Lorena Norambuena, Jiří Friml, Gabriel León, and Ricardo Tejos. “Phosphatidylinositol 4-Phosphate 5-Kinases 1 and 2 Are Involved in the Regulation of Vacuole Morphology during Arabidopsis Thaliana Pollen Development.” <i>Plant Science</i>. Elsevier, 2016. <a href=\"https://doi.org/10.1016/j.plantsci.2016.05.014\">https://doi.org/10.1016/j.plantsci.2016.05.014</a>.","mla":"Ugalde, José, et al. “Phosphatidylinositol 4-Phosphate 5-Kinases 1 and 2 Are Involved in the Regulation of Vacuole Morphology during Arabidopsis Thaliana Pollen Development.” <i>Plant Science</i>, vol. 250, Elsevier, 2016, pp. 10–19, doi:<a href=\"https://doi.org/10.1016/j.plantsci.2016.05.014\">10.1016/j.plantsci.2016.05.014</a>."},"day":"01","intvolume":"       250","publist_id":"5797","date_published":"2016-09-01T00:00:00Z","date_updated":"2025-09-18T14:29:16Z","external_id":{"isi":["000381545000002"],"pmid":["27457979"]},"status":"public","ddc":["581"],"department":[{"_id":"JiFr"}],"publication":"Plant Science","_id":"1410","pubrep_id":"1005","acknowledgement":"the Odysseus Program of the Research Foundation-Flanders [G091608] to JF.","file":[{"content_type":"application/pdf","access_level":"open_access","date_updated":"2020-07-14T12:44:53Z","file_size":4338545,"creator":"dernst","date_created":"2019-04-17T07:41:57Z","checksum":"ca08de036e6ddc81e6f760e0ccdebd3f","file_id":"6331","file_name":"2016_PlantScience_Ugalde.pdf","relation":"main_file"}],"year":"2016","page":"10 - 19","file_date_updated":"2020-07-14T12:44:53Z","publisher":"Elsevier","author":[{"last_name":"Ugalde","first_name":"José","full_name":"Ugalde, José"},{"full_name":"Rodríguez Furlán, Cecilia","first_name":"Cecilia","last_name":"Rodríguez Furlán"},{"last_name":"De Rycke","first_name":"Riet","full_name":"De Rycke, Riet"},{"first_name":"Lorena","full_name":"Norambuena, Lorena","last_name":"Norambuena"},{"first_name":"Jirí","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml"},{"last_name":"León","first_name":"Gabriel","full_name":"León, Gabriel"},{"last_name":"Tejos","full_name":"Tejos, Ricardo","first_name":"Ricardo"}],"abstract":[{"lang":"eng","text":"The pollen grains arise after meiosis of pollen mother cells within the anthers. A series of complex structural changes follows, generating mature pollen grains capable of performing the double fertilization of the female megasporophyte. Several signaling molecules, including hormones and lipids, have been involved in the regulation and appropriate control of pollen development. Phosphatidylinositol 4-phophate 5-kinases (PIP5K), which catalyze the biosynthesis of the phosphoinositide PtdIns(4,5)P2, are important for tip polar growth of root hairs and pollen tubes, embryo development, vegetative plant growth, and responses to the environment. Here, we report a role of PIP5Ks during microgametogenesis. PIP5K1 and PIP5K2 are expressed during early stages of pollen development and their transcriptional activity respond to auxin in pollen grains. Early male gametophytic lethality to certain grade was observed in both pip5k1-/- and pip5k2-/- single mutants. The number of pip5k mutant alleles is directly related to the frequency of aborted pollen grains suggesting the two genes are involved in the same function. Indeed PIP5K1 and PIP5K2 are functionally redundant since homozygous double mutants did not render viable pollen grains. The loss of function of PIP5K1 and PIP5K2results in defects in vacuole morphology in pollen at the later stages and epidermal root cells. Our results show that PIP5K1, PIP5K2 and phosphoinositide signaling are important cues for early developmental stages and vacuole formation during microgametogenesis."}]},{"month":"07","title":"PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis","language":[{"iso":"eng"}],"has_accepted_license":"1","oa":1,"issue":"1","oa_version":"Submitted Version","quality_controlled":"1","article_processing_charge":"No","citation":{"apa":"Simon, S., Skůpa, P., Viaene, T., Zwiewka, M., Tejos, R., Klíma, P., … Friml, J. (2016). PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. <i>New Phytologist</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/nph.14019\">https://doi.org/10.1111/nph.14019</a>","ieee":"S. Simon <i>et al.</i>, “PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis,” <i>New Phytologist</i>, vol. 211, no. 1. Wiley-Blackwell, pp. 65–74, 2016.","ista":"Simon S, Skůpa P, Viaene T, Zwiewka M, Tejos R, Klíma P, Čarná M, Rolčík J, De Rycke R, Moreno I, Dobrev P, Orellana A, Zažímalová E, Friml J. 2016. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. New Phytologist. 211(1), 65–74.","chicago":"Simon, Sibu, Petr Skůpa, Tom Viaene, Marta Zwiewka, Ricardo Tejos, Petr Klíma, Mária Čarná, et al. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” <i>New Phytologist</i>. Wiley-Blackwell, 2016. <a href=\"https://doi.org/10.1111/nph.14019\">https://doi.org/10.1111/nph.14019</a>.","mla":"Simon, Sibu, et al. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” <i>New Phytologist</i>, vol. 211, no. 1, Wiley-Blackwell, 2016, pp. 65–74, doi:<a href=\"https://doi.org/10.1111/nph.14019\">10.1111/nph.14019</a>.","ama":"Simon S, Skůpa P, Viaene T, et al. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. <i>New Phytologist</i>. 2016;211(1):65-74. doi:<a href=\"https://doi.org/10.1111/nph.14019\">10.1111/nph.14019</a>","short":"S. Simon, P. Skůpa, T. Viaene, M. Zwiewka, R. Tejos, P. Klíma, M. Čarná, J. Rolčík, R. De Rycke, I. Moreno, P. Dobrev, A. Orellana, E. Zažímalová, J. Friml, New Phytologist 211 (2016) 65–74."},"isi":1,"publication_status":"published","scopus_import":"1","volume":211,"type":"journal_article","doi":"10.1111/nph.14019","date_created":"2018-12-11T11:51:54Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","corr_author":"1","file":[{"file_id":"5016","creator":"system","date_created":"2018-12-12T10:13:32Z","checksum":"23522ced3508ffe7a4f247c4230e6493","date_updated":"2020-07-14T12:44:53Z","file_size":3828383,"content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_name":"IST-2018-1004-v1+1_Simon_NewPhytol_2016_proof.pdf"}],"acknowledgement":"This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP, project CEITEC (CZ.1.05/1.1.00/02.0068) and the Czech Science Foundation GACR (project no. 13-4063 7S to J.F.)","pubrep_id":"1004","_id":"1417","publisher":"Wiley-Blackwell","abstract":[{"lang":"eng","text":"Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport."}],"author":[{"first_name":"Sibu","full_name":"Simon, Sibu","last_name":"Simon","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741"},{"last_name":"Skůpa","first_name":"Petr","full_name":"Skůpa, Petr"},{"first_name":"Tom","full_name":"Viaene, Tom","last_name":"Viaene"},{"last_name":"Zwiewka","full_name":"Zwiewka, Marta","first_name":"Marta"},{"last_name":"Tejos","full_name":"Tejos, Ricardo","first_name":"Ricardo"},{"last_name":"Klíma","full_name":"Klíma, Petr","first_name":"Petr"},{"last_name":"Čarná","full_name":"Čarná, Mária","first_name":"Mária"},{"last_name":"Rolčík","first_name":"Jakub","full_name":"Rolčík, Jakub"},{"first_name":"Riet","full_name":"De Rycke, Riet","last_name":"De Rycke"},{"full_name":"Moreno, Ignacio","first_name":"Ignacio","last_name":"Moreno"},{"full_name":"Dobrev, Petre","first_name":"Petre","last_name":"Dobrev"},{"first_name":"Ariel","full_name":"Orellana, Ariel","last_name":"Orellana"},{"full_name":"Zažímalová, Eva","first_name":"Eva","last_name":"Zažímalová"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí"}],"page":"65 - 74","year":"2016","file_date_updated":"2020-07-14T12:44:53Z","external_id":{"isi":["000379212800008"]},"date_updated":"2025-09-18T14:23:21Z","ddc":["581"],"status":"public","day":"01","intvolume":"       211","date_published":"2016-07-01T00:00:00Z","publist_id":"5790","publication":"New Phytologist","department":[{"_id":"JiFr"}]},{"intvolume":"        11","day":"01","date_published":"2016-02-01T00:00:00Z","publist_id":"5711","date_updated":"2025-09-18T11:35:51Z","external_id":{"isi":["000370054100011"]},"ddc":["570"],"status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"publication":"PLoS One","department":[{"_id":"JiFr"}],"article_number":"e0147830","_id":"1482","file":[{"relation":"main_file","file_name":"IST-2016-521-v1+1_journal.pone.0147830.PDF","file_id":"5066","checksum":"6066146e527335030f83aa5924ab72a6","creator":"system","date_created":"2018-12-12T10:14:16Z","date_updated":"2020-07-14T12:44:57Z","file_size":4297148,"access_level":"open_access","content_type":"application/pdf"}],"acknowledgement":"We thank J. Traas, B. Müller and V. Reddy for providing seed materials and Y. Deb for advice regarding the laser ablation experiments. We specially thank Thomas Laux for stimulating discussions and support in the initial phase of this project.","pubrep_id":"521","year":"2016","file_date_updated":"2020-07-14T12:44:57Z","publisher":"Public Library of Science","abstract":[{"text":"Plants have the ability to continously generate new organs by maintaining populations of stem cells throught their lives. The shoot apical meristem (SAM) provides a stable environment for the maintenance of stem cells. All cells inside the SAM divide, yet boundaries and patterns are maintained. Experimental evidence indicates that patterning is independent of cell lineage, thus a dynamic self-regulatory mechanism is required. A pivotal role in the organization of the SAM is played by the WUSCHEL gene (WUS). An important question in this regard is that how WUS expression is positioned in the SAM via a cell-lineage independent signaling mechanism. In this study we demonstrate via mathematical modeling that a combination of an inhibitor of the Cytokinin (CK) receptor, Arabidopsis histidine kinase 4 (AHK4) and two morphogens originating from the top cell layer, can plausibly account for the cell lineage-independent centering of WUS expression within SAM. Furthermore, our laser ablation and microsurgical experiments support the hypothesis that patterning in SAM occurs at the level of CK reception and signaling. The model suggests that the interplay between CK signaling, WUS/CLV feedback loop and boundary signals can account for positioning of the WUS expression, and provides directions for further experimental investigation.","lang":"eng"}],"author":[{"full_name":"Adibi, Milad","first_name":"Milad","last_name":"Adibi"},{"full_name":"Yoshida, Saiko","first_name":"Saiko","last_name":"Yoshida","id":"2E46069C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Weijers, Dolf","first_name":"Dolf","last_name":"Weijers"},{"first_name":"Christian","full_name":"Fleck, Christian","last_name":"Fleck"}],"doi":"10.1371/journal.pone.0147830","date_created":"2018-12-11T11:52:17Z","scopus_import":"1","volume":11,"type":"journal_article","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","has_accepted_license":"1","oa":1,"issue":"2","oa_version":"Published Version","title":"Centering the organizing center in the Arabidopsis thaliana shoot apical meristem by a combination of cytokinin signaling and self-organization","month":"02","language":[{"iso":"eng"}],"publication_status":"published","quality_controlled":"1","article_processing_charge":"No","citation":{"mla":"Adibi, Milad, et al. “Centering the Organizing Center in the Arabidopsis Thaliana Shoot Apical Meristem by a Combination of Cytokinin Signaling and Self-Organization.” <i>PLoS One</i>, vol. 11, no. 2, e0147830, Public Library of Science, 2016, doi:<a href=\"https://doi.org/10.1371/journal.pone.0147830\">10.1371/journal.pone.0147830</a>.","chicago":"Adibi, Milad, Saiko Yoshida, Dolf Weijers, and Christian Fleck. “Centering the Organizing Center in the Arabidopsis Thaliana Shoot Apical Meristem by a Combination of Cytokinin Signaling and Self-Organization.” <i>PLoS One</i>. Public Library of Science, 2016. <a href=\"https://doi.org/10.1371/journal.pone.0147830\">https://doi.org/10.1371/journal.pone.0147830</a>.","apa":"Adibi, M., Yoshida, S., Weijers, D., &#38; Fleck, C. (2016). Centering the organizing center in the Arabidopsis thaliana shoot apical meristem by a combination of cytokinin signaling and self-organization. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0147830\">https://doi.org/10.1371/journal.pone.0147830</a>","ieee":"M. Adibi, S. Yoshida, D. Weijers, and C. Fleck, “Centering the organizing center in the Arabidopsis thaliana shoot apical meristem by a combination of cytokinin signaling and self-organization,” <i>PLoS One</i>, vol. 11, no. 2. Public Library of Science, 2016.","ista":"Adibi M, Yoshida S, Weijers D, Fleck C. 2016. Centering the organizing center in the Arabidopsis thaliana shoot apical meristem by a combination of cytokinin signaling and self-organization. PLoS One. 11(2), e0147830.","short":"M. Adibi, S. Yoshida, D. Weijers, C. Fleck, PLoS One 11 (2016).","ama":"Adibi M, Yoshida S, Weijers D, Fleck C. Centering the organizing center in the Arabidopsis thaliana shoot apical meristem by a combination of cytokinin signaling and self-organization. <i>PLoS One</i>. 2016;11(2). doi:<a href=\"https://doi.org/10.1371/journal.pone.0147830\">10.1371/journal.pone.0147830</a>"},"isi":1},{"publication_status":"published","citation":{"mla":"Chen, Xu, et al. “Environmental and Endogenous Control of Cortical Microtubule Orientation.” <i>Trends in Cell Biology</i>, vol. 26, no. 6, Cell Press, 2016, pp. 409–19, doi:<a href=\"https://doi.org/10.1016/j.tcb.2016.02.003\">10.1016/j.tcb.2016.02.003</a>.","chicago":"Chen, Xu, Shuang Wu, Zengyu Liu, and Jiří Friml. “Environmental and Endogenous Control of Cortical Microtubule Orientation.” <i>Trends in Cell Biology</i>. Cell Press, 2016. <a href=\"https://doi.org/10.1016/j.tcb.2016.02.003\">https://doi.org/10.1016/j.tcb.2016.02.003</a>.","apa":"Chen, X., Wu, S., Liu, Z., &#38; Friml, J. (2016). Environmental and endogenous control of cortical microtubule orientation. <i>Trends in Cell Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.tcb.2016.02.003\">https://doi.org/10.1016/j.tcb.2016.02.003</a>","ieee":"X. Chen, S. Wu, Z. Liu, and J. Friml, “Environmental and endogenous control of cortical microtubule orientation,” <i>Trends in Cell Biology</i>, vol. 26, no. 6. Cell Press, pp. 409–419, 2016.","ista":"Chen X, Wu S, Liu Z, Friml J. 2016. Environmental and endogenous control of cortical microtubule orientation. Trends in Cell Biology. 26(6), 409–419.","short":"X. Chen, S. Wu, Z. Liu, J. Friml, Trends in Cell Biology 26 (2016) 409–419.","ama":"Chen X, Wu S, Liu Z, Friml J. Environmental and endogenous control of cortical microtubule orientation. <i>Trends in Cell Biology</i>. 2016;26(6):409-419. doi:<a href=\"https://doi.org/10.1016/j.tcb.2016.02.003\">10.1016/j.tcb.2016.02.003</a>"},"isi":1,"quality_controlled":"1","article_processing_charge":"No","issue":"6","oa_version":"Submitted Version","oa":1,"has_accepted_license":"1","language":[{"iso":"eng"}],"title":"Environmental and endogenous control of cortical microtubule orientation","month":"06","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2018-12-11T11:52:17Z","doi":"10.1016/j.tcb.2016.02.003","type":"journal_article","volume":26,"article_type":"review","scopus_import":"1","file_date_updated":"2020-07-14T12:44:57Z","year":"2016","page":"409 - 419","author":[{"first_name":"Xu","full_name":"Chen, Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","last_name":"Chen"},{"full_name":"Wu, Shuang","first_name":"Shuang","last_name":"Wu"},{"first_name":"Zengyu","full_name":"Liu, Zengyu","last_name":"Liu"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiřĺ","full_name":"Friml, Jiřĺ"}],"publisher":"Cell Press","_id":"1484","pubrep_id":"1002","file":[{"date_updated":"2020-07-14T12:44:57Z","file_size":2329117,"access_level":"open_access","content_type":"application/pdf","file_id":"5155","checksum":"b229e5bb4676ec3e27b7b9ea603b3a63","date_created":"2018-12-12T10:15:34Z","creator":"system","file_name":"IST-2018-1002-v1+1_Chen_TICB_2016_proofs.pdf","relation":"main_file"}],"acknowledgement":"We thank Maciek Adamowski for helpful discussions and Qiang Zhu and Israel Ausin for critical reading of the manuscript. We sincerely apologize to colleagues whose work we could not include owing to space limitations.","department":[{"_id":"JiFr"}],"publication":"Trends in Cell Biology","publist_id":"5704","date_published":"2016-06-01T00:00:00Z","day":"01","intvolume":"        26","status":"public","ddc":["581"],"date_updated":"2025-09-18T11:35:11Z","external_id":{"isi":["000376807500004"]}},{"issue":"2","oa_version":"Preprint","oa":1,"has_accepted_license":"1","language":[{"iso":"eng"}],"month":"06","title":"A forward genetic screen for new regulators of auxin mediated degradation of auxin transport proteins in Arabidopsis thaliana","publication_status":"published","citation":{"ista":"Zemová R, Zwiewka M, Bielach A, Robert H, Friml J. 2016. A forward genetic screen for new regulators of auxin mediated degradation of auxin transport proteins in Arabidopsis thaliana. Journal of Plant Growth Regulation. 35(2), 465–476.","ieee":"R. Zemová, M. Zwiewka, A. Bielach, H. Robert, and J. Friml, “A forward genetic screen for new regulators of auxin mediated degradation of auxin transport proteins in Arabidopsis thaliana,” <i>Journal of Plant Growth Regulation</i>, vol. 35, no. 2. Springer, pp. 465–476, 2016.","apa":"Zemová, R., Zwiewka, M., Bielach, A., Robert, H., &#38; Friml, J. (2016). A forward genetic screen for new regulators of auxin mediated degradation of auxin transport proteins in Arabidopsis thaliana. <i>Journal of Plant Growth Regulation</i>. Springer. <a href=\"https://doi.org/10.1007/s00344-015-9553-2\">https://doi.org/10.1007/s00344-015-9553-2</a>","chicago":"Zemová, Radka, Marta Zwiewka, Agnieszka Bielach, Hélène Robert, and Jiří Friml. “A Forward Genetic Screen for New Regulators of Auxin Mediated Degradation of Auxin Transport Proteins in Arabidopsis Thaliana.” <i>Journal of Plant Growth Regulation</i>. Springer, 2016. <a href=\"https://doi.org/10.1007/s00344-015-9553-2\">https://doi.org/10.1007/s00344-015-9553-2</a>.","mla":"Zemová, Radka, et al. “A Forward Genetic Screen for New Regulators of Auxin Mediated Degradation of Auxin Transport Proteins in Arabidopsis Thaliana.” <i>Journal of Plant Growth Regulation</i>, vol. 35, no. 2, Springer, 2016, pp. 465–76, doi:<a href=\"https://doi.org/10.1007/s00344-015-9553-2\">10.1007/s00344-015-9553-2</a>.","ama":"Zemová R, Zwiewka M, Bielach A, Robert H, Friml J. A forward genetic screen for new regulators of auxin mediated degradation of auxin transport proteins in Arabidopsis thaliana. <i>Journal of Plant Growth Regulation</i>. 2016;35(2):465-476. doi:<a href=\"https://doi.org/10.1007/s00344-015-9553-2\">10.1007/s00344-015-9553-2</a>","short":"R. Zemová, M. Zwiewka, A. Bielach, H. Robert, J. Friml, Journal of Plant Growth Regulation 35 (2016) 465–476."},"isi":1,"article_processing_charge":"No","quality_controlled":"1","date_created":"2018-12-11T11:53:12Z","doi":"10.1007/s00344-015-9553-2","type":"journal_article","volume":35,"scopus_import":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"1641","pubrep_id":"1001","file":[{"file_id":"4695","checksum":"0dc6a300cde6536ceedd2bcdd2060efb","creator":"system","date_created":"2018-12-12T10:08:34Z","date_updated":"2020-07-14T12:45:08Z","file_size":5637591,"access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_name":"IST-2018-1001-v1+1_Zemova_JPlantGrowthRegul_2016_proofs.pdf"}],"acknowledgement":"European Social Fund (CZ.1.07/2.3.00/20.0043) and the Czech Science Foundation GAČR (GA13-40637S) to JF. ","corr_author":"1","file_date_updated":"2020-07-14T12:45:08Z","page":"465 - 476","year":"2016","author":[{"first_name":"Radka","full_name":"Zemová, Radka","last_name":"Zemová"},{"last_name":"Zwiewka","full_name":"Zwiewka, Marta","first_name":"Marta"},{"last_name":"Bielach","first_name":"Agnieszka","full_name":"Bielach, Agnieszka"},{"full_name":"Robert, Hélène","first_name":"Hélène","last_name":"Robert"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","first_name":"Jirí"}],"abstract":[{"lang":"eng","text":"The plant hormone auxin (indole-3-acetic acid) is a major regulator of plant growth and development including embryo and root patterning, lateral organ formation and growth responses to environmental stimuli. Auxin is directionally transported from cell to cell by the action of specific auxin influx [AUXIN-RESISTANT1 (AUX1)] and efflux [PIN-FORMED (PIN)] transport regulators, whose polar, subcellular localizations are aligned with the direction of the auxin flow. Auxin itself regulates its own transport by modulation of the expression and subcellular localization of the auxin transporters. Increased auxin levels promote the transcription of PIN2 and AUX1 genes as well as stabilize PIN proteins at the plasma membrane, whereas prolonged auxin exposure increases the turnover of PIN proteins and their degradation in the vacuole. In this study, we applied a forward genetic approach, to identify molecular components playing a role in the auxin-mediated degradation. We generated EMS-mutagenized Arabidopsis PIN2::PIN2:GFP, AUX1::AUX1:YFP eir1aux1 populations and designed a screen for mutants with persistently strong fluorescent signals of the tagged PIN2 and AUX1 after prolonged treatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D). This approach yielded novel auxin degradation mutants defective in trafficking and degradation of PIN2 and AUX1 proteins and established a role for auxin-mediated degradation in plant development."}],"publisher":"Springer","publist_id":"5512","date_published":"2016-06-01T00:00:00Z","intvolume":"        35","day":"01","status":"public","ddc":["581"],"date_updated":"2025-09-18T10:51:26Z","external_id":{"isi":["000376482300015"]},"department":[{"_id":"JiFr"}],"publication":"Journal of Plant Growth Regulation"},{"title":"The CLE gene family in Populus trichocarpa","month":"06","language":[{"iso":"eng"}],"issue":"6","oa_version":"Submitted Version","oa":1,"article_processing_charge":"No","quality_controlled":"1","isi":1,"citation":{"chicago":"Liu, Zhijun, Nan Yang, Yanting Lv, Lixia Pan, Shuo Lv, Huibin Han, and Guodong Wang. “The CLE Gene Family in Populus Trichocarpa.” <i>Plant Signaling &#38; Behavior</i>. Taylor &#38; Francis, 2016. <a href=\"https://doi.org/10.1080/15592324.2016.1191734\">https://doi.org/10.1080/15592324.2016.1191734</a>.","mla":"Liu, Zhijun, et al. “The CLE Gene Family in Populus Trichocarpa.” <i>Plant Signaling &#38; Behavior</i>, vol. 11, no. 6, e1191734, Taylor &#38; Francis, 2016, doi:<a href=\"https://doi.org/10.1080/15592324.2016.1191734\">10.1080/15592324.2016.1191734</a>.","ista":"Liu Z, Yang N, Lv Y, Pan L, Lv S, Han H, Wang G. 2016. The CLE gene family in Populus trichocarpa. Plant Signaling &#38; Behavior. 11(6), e1191734.","ieee":"Z. Liu <i>et al.</i>, “The CLE gene family in Populus trichocarpa,” <i>Plant Signaling &#38; Behavior</i>, vol. 11, no. 6. Taylor &#38; Francis, 2016.","apa":"Liu, Z., Yang, N., Lv, Y., Pan, L., Lv, S., Han, H., &#38; Wang, G. (2016). The CLE gene family in Populus trichocarpa. <i>Plant Signaling &#38; Behavior</i>. Taylor &#38; Francis. <a href=\"https://doi.org/10.1080/15592324.2016.1191734\">https://doi.org/10.1080/15592324.2016.1191734</a>","short":"Z. Liu, N. Yang, Y. Lv, L. Pan, S. Lv, H. Han, G. Wang, Plant Signaling &#38; Behavior 11 (2016).","ama":"Liu Z, Yang N, Lv Y, et al. The CLE gene family in Populus trichocarpa. <i>Plant Signaling &#38; Behavior</i>. 2016;11(6). doi:<a href=\"https://doi.org/10.1080/15592324.2016.1191734\">10.1080/15592324.2016.1191734</a>"},"publication_status":"published","volume":11,"scopus_import":"1","type":"journal_article","doi":"10.1080/15592324.2016.1191734","date_created":"2018-12-11T11:46:53Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","acknowledgement":"We are grateful to Dr. Long (Laboratoire de Reproduction et Developpement des Plantes,CNRS,INRA,ENSLyon,UCBL,Universite de Lyon,France)for critical reading of the article. Work in our group is supported by the National Natural Science Foundation of China (31271575; 31200902), the Fundamental Research Funds for the Central Univ ersities (GK201103005), the Specialized Research Fund for the Doctoral Program of Higher Education from the Ministry of Education of China (20120202120009), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and the Natural Science Basic Research Plan in Shaanxi Province of China (2014JM3064). ","article_number":"e1191734","_id":"510","publisher":"Taylor & Francis","author":[{"first_name":"Zhijun","full_name":"Liu, Zhijun","last_name":"Liu"},{"last_name":"Yang","first_name":"Nan","full_name":"Yang, Nan"},{"last_name":"Lv","full_name":"Lv, Yanting","first_name":"Yanting"},{"last_name":"Pan","first_name":"Lixia","full_name":"Pan, Lixia"},{"full_name":"Lv, Shuo","first_name":"Shuo","last_name":"Lv"},{"last_name":"Han","id":"31435098-F248-11E8-B48F-1D18A9856A87","full_name":"Han, Huibin","first_name":"Huibin"},{"last_name":"Wang","full_name":"Wang, Guodong","first_name":"Guodong"}],"abstract":[{"text":"The CLE (CLAVATA3/Embryo Surrounding Region-related) peptides are small secreted signaling peptides that are primarily involved in the regulation of stem cell homeostasis in different plant meristems. Particularly, the characterization of the CLE41-PXY/TDR signaling pathway has greatly advanced our understanding on the potential roles of CLE peptides in vascular development and wood formation. Nevertheless, our knowledge on this gene family in a tree species is limited. In a recent study, we reported on a systematically investigation of the CLE gene family in Populus trichocarpa . The potential roles of PtCLE genes were studied by comparative analysis and transcriptional pro fi ling. Among fi fty PtCLE members, many PtCLE proteins share identical CLE motifs or contain the same CLE motif as that of AtCLEs, while PtCLE genes exhibited either comparable or distinct expression patterns comparing to their Arabidopsis counterparts. These fi ndings indicate the existence of both functional conservation and functional divergence between PtCLEs and their AtCLE orthologues. Our results provide valuable resources for future functional investigations of these critical signaling molecules in woody plants. ","lang":"eng"}],"year":"2016","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973754/","open_access":"1"}],"date_updated":"2025-09-22T14:21:19Z","external_id":{"isi":["000378740600025"]},"status":"public","intvolume":"        11","day":"02","publist_id":"7308","date_published":"2016-06-02T00:00:00Z","publication":"Plant Signaling & Behavior","department":[{"_id":"JiFr"}]},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.1038/celldisc.2016.18","date_created":"2018-12-11T11:50:02Z","volume":2,"scopus_import":"1","type":"journal_article","publication_status":"published","article_processing_charge":"No","quality_controlled":"1","project":[{"grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants"}],"isi":1,"citation":{"apa":"Łangowski, Ł., Wabnik, K. T., Li, H., Vanneste, S., Naramoto, S., Tanaka, H., &#38; Friml, J. (2016). Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. <i>Cell Discovery</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/celldisc.2016.18\">https://doi.org/10.1038/celldisc.2016.18</a>","ieee":"Ł. Łangowski <i>et al.</i>, “Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells,” <i>Cell Discovery</i>, vol. 2. Nature Publishing Group, 2016.","ista":"Łangowski Ł, Wabnik KT, Li H, Vanneste S, Naramoto S, Tanaka H, Friml J. 2016. Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. Cell Discovery. 2, 16018.","mla":"Łangowski, Łukasz, et al. “Cellular Mechanisms for Cargo Delivery and Polarity Maintenance at Different Polar Domains in Plant Cells.” <i>Cell Discovery</i>, vol. 2, 16018, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/celldisc.2016.18\">10.1038/celldisc.2016.18</a>.","chicago":"Łangowski, Łukasz, Krzysztof T Wabnik, Hongjiang Li, Steffen Vanneste, Satoshi Naramoto, Hirokazu Tanaka, and Jiří Friml. “Cellular Mechanisms for Cargo Delivery and Polarity Maintenance at Different Polar Domains in Plant Cells.” <i>Cell Discovery</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/celldisc.2016.18\">https://doi.org/10.1038/celldisc.2016.18</a>.","ama":"Łangowski Ł, Wabnik KT, Li H, et al. Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. <i>Cell Discovery</i>. 2016;2. doi:<a href=\"https://doi.org/10.1038/celldisc.2016.18\">10.1038/celldisc.2016.18</a>","short":"Ł. Łangowski, K.T. Wabnik, H. Li, S. Vanneste, S. Naramoto, H. Tanaka, J. Friml, Cell Discovery 2 (2016)."},"has_accepted_license":"1","oa_version":"Published Version","oa":1,"month":"07","ec_funded":1,"title":"Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells","language":[{"iso":"eng"}],"department":[{"_id":"EvBe"},{"_id":"JiFr"}],"publication":"Cell Discovery","intvolume":"         2","day":"19","publist_id":"6299","date_published":"2016-07-19T00:00:00Z","external_id":{"isi":["000414797400001"]},"date_updated":"2025-09-22T14:19:46Z","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"ddc":["580"],"year":"2016","file_date_updated":"2018-12-12T10:13:33Z","publisher":"Nature Publishing Group","author":[{"full_name":"Łangowski, Łukasz","first_name":"Łukasz","last_name":"Łangowski"},{"orcid":"0000-0001-7263-0560","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","last_name":"Wabnik","full_name":"Wabnik, Krzysztof T","first_name":"Krzysztof T"},{"first_name":"Hongjiang","full_name":"Li, Hongjiang","last_name":"Li","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5039-9660"},{"last_name":"Vanneste","full_name":"Vanneste, Steffen","first_name":"Steffen"},{"first_name":"Satoshi","full_name":"Naramoto, Satoshi","last_name":"Naramoto"},{"last_name":"Tanaka","first_name":"Hirokazu","full_name":"Tanaka, Hirokazu"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"abstract":[{"text":"The asymmetric localization of proteins in the plasma membrane domains of eukaryotic cells is a fundamental manifestation of cell polarity that is central to multicellular organization and developmental patterning. In plants, the mechanisms underlying the polar localization of cargo proteins are still largely unknown and appear to be fundamentally distinct from those operating in mammals. Here, we present a systematic, quantitative comparative analysis of the polar delivery and subcellular localization of proteins that characterize distinct polar plasma membrane domains in plant cells. The combination of microscopic analyses and computational modeling revealed a mechanistic framework common to diverse polar cargos and underlying the establishment and maintenance of apical, basal, and lateral polar domains in plant cells. This mechanism depends on the polar secretion, constitutive endocytic recycling, and restricted lateral diffusion of cargos within the plasma membrane. Moreover, our observations suggest that polar cargo distribution involves the individual protein potential to form clusters within the plasma membrane and interact with the extracellular matrix. Our observations provide insights into the shared cellular mechanisms of polar cargo delivery and polarity maintenance in plant cells.","lang":"eng"}],"article_number":"16018","_id":"1081","pubrep_id":"757","acknowledgement":"We thank Bonnie Bartel, Jenny Russinova and Niko Geldner\r\nfor sharing published material, Martine de Cock and Annick\r\nBleys for help in preparing the manuscript. This work was\r\nsupported by the European Research Council (project\r\nERC-2011-StG-20101109-PSDP); Czech Science Foundation\r\nGAČR (GA13-40637S); project CEITEC—Central European\r\nInstitute of Technology (CZ.1.05/1.1.00/02.0068). SV is a\r\npostdoctoral fellow of the Research Foundation-Flanders.\r\nSN is a Project Assistant Professor supported by the Japanese\r\nSociety for the Promotion of Science (JSPS; 30612022 to SN),\r\nthe NC-CARP project of the Ministry of Education, Culture,\r\nSports, Science and Technology in Japan to SN.","file":[{"file_id":"5017","date_created":"2018-12-12T10:13:33Z","creator":"system","date_updated":"2018-12-12T10:13:33Z","file_size":5261671,"access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_name":"IST-2017-757-v1+1_celldisc201618.pdf"}]}]