[{"day":"01","author":[{"full_name":"Naramoto, Satoshi","last_name":"Naramoto","first_name":"Satoshi"},{"full_name":"Nodzyński, Tomasz","last_name":"Nodzyński","first_name":"Tomasz"},{"last_name":"Dainobu","full_name":"Dainobu, Tomoko","first_name":"Tomoko"},{"full_name":"Takatsuka, Hirotomo","last_name":"Takatsuka","first_name":"Hirotomo"},{"first_name":"Teruyo","last_name":"Okada","full_name":"Okada, Teruyo"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","first_name":"Jirí"},{"first_name":"Hiroo","full_name":"Fukuda, Hiroo","last_name":"Fukuda"}],"language":[{"iso":"eng"}],"project":[{"grant_number":"282300","call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"status":"public","external_id":{"isi":["000334679500009"]},"abstract":[{"lang":"eng","text":"Leaf venation develops complex patterns in angiosperms, but the mechanism underlying this process is largely unknown. To elucidate the molecular mechanisms governing vein pattern formation, we previously isolated vascular network defective (van) mutants that displayed venation discontinuities. Here, we report the phenotypic analysis of van4 mutants, and we identify and characterize the VAN4 gene. Detailed phenotypic analysis shows that van4 mutants are defective in procambium cell differentiation and subsequent vascular cell differentiation. Reduced shoot and root cell growth is observed in van4 mutants, suggesting that VAN4 function is important for cell growth and the establishment of venation continuity. Consistent with these phenotypes, the VAN4 gene is strongly expressed in vascular and meristematic cells. VAN4 encodes a putative TRS120, which is a known guanine nucleotide exchange factor (GEF) for Rab GTPase involved in regulating vesicle transport, and a known tethering factor that determines the specificity of membrane fusion. VAN4 protein localizes at the trans-Golgi network/early endosome (TGN/EE). Aberrant recycling of the auxin efflux carrier PIN proteins is observed in van4 mutants. These results suggest that VAN4-mediated exocytosis at the TGN plays important roles in plant vascular development and cell growth in shoot and root. Our identification of VAN4 as a putative TRS120 shows that Rab GTPases are crucial (in addition to ARF GTPases) for continuous vascular development, and provides further evidence for the importance of vesicle transport in leaf vascular formation."}],"issue":"4","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","ec_funded":1,"citation":{"ista":"Naramoto S, Nodzyński T, Dainobu T, Takatsuka H, Okada T, Friml J, Fukuda H. 2014. VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis. Plant and Cell Physiology. 55(4), 750–763.","chicago":"Naramoto, Satoshi, Tomasz Nodzyński, Tomoko Dainobu, Hirotomo Takatsuka, Teruyo Okada, Jiří Friml, and Hiroo Fukuda. “VAN4 Encodes a Putative TRS120 That Is Required for Normal Cell Growth and Vein Development in Arabidopsis.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2014. <a href=\"https://doi.org/10.1093/pcp/pcu012\">https://doi.org/10.1093/pcp/pcu012</a>.","ama":"Naramoto S, Nodzyński T, Dainobu T, et al. VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis. <i>Plant and Cell Physiology</i>. 2014;55(4):750-763. doi:<a href=\"https://doi.org/10.1093/pcp/pcu012\">10.1093/pcp/pcu012</a>","ieee":"S. Naramoto <i>et al.</i>, “VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis,” <i>Plant and Cell Physiology</i>, vol. 55, no. 4. Oxford University Press, pp. 750–763, 2014.","mla":"Naramoto, Satoshi, et al. “VAN4 Encodes a Putative TRS120 That Is Required for Normal Cell Growth and Vein Development in Arabidopsis.” <i>Plant and Cell Physiology</i>, vol. 55, no. 4, Oxford University Press, 2014, pp. 750–63, doi:<a href=\"https://doi.org/10.1093/pcp/pcu012\">10.1093/pcp/pcu012</a>.","apa":"Naramoto, S., Nodzyński, T., Dainobu, T., Takatsuka, H., Okada, T., Friml, J., &#38; Fukuda, H. (2014). VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcu012\">https://doi.org/10.1093/pcp/pcu012</a>","short":"S. Naramoto, T. Nodzyński, T. Dainobu, H. Takatsuka, T. Okada, J. Friml, H. Fukuda, Plant and Cell Physiology 55 (2014) 750–763."},"department":[{"_id":"JiFr"}],"_id":"2222","scopus_import":"1","publisher":"Oxford University Press","page":"750 - 763","quality_controlled":"1","oa_version":"None","doi":"10.1093/pcp/pcu012","date_published":"2014-04-01T00:00:00Z","article_processing_charge":"No","title":"VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis","month":"04","volume":55,"isi":1,"intvolume":"        55","date_updated":"2025-09-29T11:28:27Z","date_created":"2018-12-11T11:56:24Z","fulldoi":"https://doi.org/10.1093/pcp/pcu012","publist_id":"4742","publication_identifier":{"issn":["0032-0781"]},"publication_status":"published","publication":"Plant and Cell Physiology","year":"2014","type":"journal_article"},{"language":[{"iso":"eng"}],"author":[{"full_name":"Tanaka, Hirokazu","last_name":"Tanaka","first_name":"Hirokazu"},{"last_name":"Nodzyński","full_name":"Nodzyński, Tomasz","first_name":"Tomasz"},{"full_name":"Kitakura, Saeko","last_name":"Kitakura","first_name":"Saeko"},{"last_name":"Feraru","full_name":"Feraru, Mugurel","first_name":"Mugurel"},{"last_name":"Sasabe","full_name":"Sasabe, Michiko","first_name":"Michiko"},{"last_name":"Ishikawa","full_name":"Ishikawa, Tomomi","first_name":"Tomomi"},{"first_name":"Jürgen","full_name":"Kleine Vehn, Jürgen","last_name":"Kleine Vehn"},{"first_name":"Tatsuo","full_name":"Kakimoto, Tatsuo","last_name":"Kakimoto"},{"full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí","orcid":"0000-0002-8302-7596"}],"file_date_updated":"2020-07-14T12:45:34Z","pubrep_id":"431","day":"01","project":[{"call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300"},{"_id":"256BDAB0-B435-11E9-9278-68D0E5697425","name":"Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur"}],"status":"public","external_id":{"pmid":["24369434"],"isi":["000334679500008"]},"abstract":[{"lang":"eng","text":"Correct positioning of membrane proteins is an essential process in eukaryotic organisms. The plant hormone auxin is distributed through intercellular transport and triggers various cellular responses. Auxin transporters of the PIN-FORMED (PIN) family localize asymmetrically at the plasma membrane (PM) and mediate the directional transport of auxin between cells. A fungal toxin, brefeldin A (BFA), inhibits a subset of guanine nucleotide exchange factors for ADP-ribosylation factor small GTPases (ARF GEFs) including GNOM, which plays a major role in localization of PIN1 predominantly to the basal side of the PM. The Arabidopsis genome encodes 19 ARF-related putative GTPases. However, ARF components involved in PIN1 localization have been genetically poorly defined. Using a fluorescence imaging-based forward genetic approach, we identified an Arabidopsis mutant, bfa-visualized exocytic trafficking defective1 (bex1), in which PM localization of PIN1-green fluorescent protein (GFP) as well as development is hypersensitive to BFA. We found that in bex1 a member of the ARF1 gene family, ARF1A1C, was mutated. ARF1A1C localizes to the trans-Golgi network/early endosome and Golgi apparatus, acts synergistically to BEN1/MIN7 ARF GEF and is important for PIN recycling to the PM. Consistent with the developmental importance of PIN proteins, functional interference with ARF1 resulted in an impaired auxin response gradient and various developmental defects including embryonic patterning defects and growth arrest. Our results show that ARF1A1C is essential for recycling of PIN auxin transporters and for various auxin-dependent developmental processes."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"4","ec_funded":1,"_id":"2223","scopus_import":"1","department":[{"_id":"JiFr"}],"citation":{"ama":"Tanaka H, Nodzyński T, Kitakura S, et al. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis. <i>Plant and Cell Physiology</i>. 2014;55(4):737-749. doi:<a href=\"https://doi.org/10.1093/pcp/pct196\">10.1093/pcp/pct196</a>","mla":"Tanaka, Hirokazu, et al. “BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling of PIN Auxin Transporters and Auxin-Mediated Development in Arabidopsis.” <i>Plant and Cell Physiology</i>, vol. 55, no. 4, Oxford University Press, 2014, pp. 737–49, doi:<a href=\"https://doi.org/10.1093/pcp/pct196\">10.1093/pcp/pct196</a>.","ieee":"H. Tanaka <i>et al.</i>, “BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis,” <i>Plant and Cell Physiology</i>, vol. 55, no. 4. Oxford University Press, pp. 737–749, 2014.","short":"H. Tanaka, T. Nodzyński, S. Kitakura, M. Feraru, M. Sasabe, T. Ishikawa, J. Kleine Vehn, T. Kakimoto, J. Friml, Plant and Cell Physiology 55 (2014) 737–749.","apa":"Tanaka, H., Nodzyński, T., Kitakura, S., Feraru, M., Sasabe, M., Ishikawa, T., … Friml, J. (2014). BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pct196\">https://doi.org/10.1093/pcp/pct196</a>","ista":"Tanaka H, Nodzyński T, Kitakura S, Feraru M, Sasabe M, Ishikawa T, Kleine Vehn J, Kakimoto T, Friml J. 2014. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis. Plant and Cell Physiology. 55(4), 737–749.","chicago":"Tanaka, Hirokazu, Tomasz Nodzyński, Saeko Kitakura, Mugurel Feraru, Michiko Sasabe, Tomomi Ishikawa, Jürgen Kleine Vehn, Tatsuo Kakimoto, and Jiří Friml. “BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling of PIN Auxin Transporters and Auxin-Mediated Development in Arabidopsis.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2014. <a href=\"https://doi.org/10.1093/pcp/pct196\">https://doi.org/10.1093/pcp/pct196</a>."},"publisher":"Oxford University Press","oa_version":"Published Version","tmp":{"short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"pmid":1,"quality_controlled":"1","ddc":["570"],"page":"737 - 749","has_accepted_license":"1","date_published":"2014-04-01T00:00:00Z","doi":"10.1093/pcp/pct196","title":"BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis","article_processing_charge":"No","month":"04","license":"https://creativecommons.org/licenses/by-nc/4.0/","oa":1,"isi":1,"volume":55,"intvolume":"        55","publication":"Plant and Cell Physiology","publication_status":"published","publist_id":"4741","publication_identifier":{"issn":["0032-0781"]},"file":[{"content_type":"application/pdf","creator":"system","relation":"main_file","access_level":"open_access","checksum":"b781a76b32ac35a520256453c3ba9433","file_size":2028111,"file_id":"5076","file_name":"IST-2016-431-v1+1_Plant_Cell_Physiol-2014-Tanaka-737-49.pdf","date_updated":"2020-07-14T12:45:34Z","date_created":"2018-12-12T10:14:25Z"}],"fulldoi":"https://doi.org/10.1093/pcp/pct196","date_created":"2018-12-11T11:56:25Z","date_updated":"2025-09-29T11:27:52Z","type":"journal_article","year":"2014"},{"article_processing_charge":"No","title":"Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences","month":"03","corr_author":"1","page":"1 - 10","oa_version":"None","quality_controlled":"1","doi":"10.1007/s13127-013-0150-6","date_published":"2014-03-01T00:00:00Z","date_updated":"2025-09-29T11:25:37Z","publication_identifier":{"issn":["1439-6092"]},"publist_id":"4734","publication_status":"published","publication":"Organisms Diversity and Evolution","date_created":"2018-12-11T11:56:26Z","fulldoi":"https://doi.org/10.1007/s13127-013-0150-6","type":"journal_article","year":"2014","isi":1,"volume":14,"intvolume":"        14","abstract":[{"lang":"eng","text":"The Balkan Peninsula, characterized by high rates of endemism, is recognised as one of the most diverse and species-rich areas of Europe. However, little is known about the origin of Balkan endemics. The present study addresses the phylogenetic position of the Balkan endemic Ranunculus wettsteinii, as well as its taxonomic status and relationship with the widespread R. parnassiifolius, based on nuclear DNA (internal transcribed spacer, ITS) and plastid regions (rpl32-trnL, rps16-trnQ, trnK-matK and ycf6-psbM). Maximum parsimony and Bayesian inference analyses revealed a well-supported clade formed by accessions of R. wettsteinii. Furthermore, our phylogenetic and network analyses supported previous hypotheses of a likely allopolyploid origin for R. wettsteinii between R. montenegrinus and R. parnassiifolius, with the latter as the maternal parent."}],"external_id":{"isi":["000332585400001"]},"day":"01","language":[{"iso":"eng"}],"author":[{"id":"2AD56A7A-F248-11E8-B48F-1D18A9856A87","full_name":"Cires Rodriguez, Eduardo","last_name":"Cires Rodriguez","first_name":"Eduardo"},{"full_name":"Baltisberger, Matthias","last_name":"Baltisberger","first_name":"Matthias"},{"id":"33A3C818-F248-11E8-B48F-1D18A9856A87","full_name":"Cuesta, Candela","last_name":"Cuesta","first_name":"Candela","orcid":"0000-0003-1923-2410"},{"first_name":"Pablo","full_name":"Vargas, Pablo","last_name":"Vargas"},{"first_name":"José","last_name":"Prieto","full_name":"Prieto, José"}],"status":"public","citation":{"short":"E. Cires Rodriguez, M. Baltisberger, C. Cuesta, P. Vargas, J. Prieto, Organisms Diversity and Evolution 14 (2014) 1–10.","apa":"Cires Rodriguez, E., Baltisberger, M., Cuesta, C., Vargas, P., &#38; Prieto, J. (2014). Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences. <i>Organisms Diversity and Evolution</i>. Springer. <a href=\"https://doi.org/10.1007/s13127-013-0150-6\">https://doi.org/10.1007/s13127-013-0150-6</a>","ama":"Cires Rodriguez E, Baltisberger M, Cuesta C, Vargas P, Prieto J. Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences. <i>Organisms Diversity and Evolution</i>. 2014;14(1):1-10. doi:<a href=\"https://doi.org/10.1007/s13127-013-0150-6\">10.1007/s13127-013-0150-6</a>","ieee":"E. Cires Rodriguez, M. Baltisberger, C. Cuesta, P. Vargas, and J. Prieto, “Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences,” <i>Organisms Diversity and Evolution</i>, vol. 14, no. 1. Springer, pp. 1–10, 2014.","mla":"Cires Rodriguez, Eduardo, et al. “Allopolyploid Origin of the Balkan Endemic Ranunculus Wettsteinii (Ranunculaceae) Inferred from Nuclear and Plastid DNA Sequences.” <i>Organisms Diversity and Evolution</i>, vol. 14, no. 1, Springer, 2014, pp. 1–10, doi:<a href=\"https://doi.org/10.1007/s13127-013-0150-6\">10.1007/s13127-013-0150-6</a>.","chicago":"Cires Rodriguez, Eduardo, Matthias Baltisberger, Candela Cuesta, Pablo Vargas, and José Prieto. “Allopolyploid Origin of the Balkan Endemic Ranunculus Wettsteinii (Ranunculaceae) Inferred from Nuclear and Plastid DNA Sequences.” <i>Organisms Diversity and Evolution</i>. Springer, 2014. <a href=\"https://doi.org/10.1007/s13127-013-0150-6\">https://doi.org/10.1007/s13127-013-0150-6</a>.","ista":"Cires Rodriguez E, Baltisberger M, Cuesta C, Vargas P, Prieto J. 2014. Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences. Organisms Diversity and Evolution. 14(1), 1–10."},"_id":"2227","scopus_import":"1","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"publisher":"Springer","issue":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"date_published":"2014-02-13T00:00:00Z","doi":"10.1016/j.cell.2014.01.039","quality_controlled":"1","oa_version":"None","page":"691 - 704","month":"02","title":"The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants","article_processing_charge":"No","intvolume":"       156","isi":1,"volume":156,"year":"2014","type":"journal_article","fulldoi":"https://doi.org/10.1016/j.cell.2014.01.039","date_created":"2018-12-11T11:56:31Z","publist_id":"4721","publication_identifier":{"issn":["0092-8674"]},"publication_status":"published","publication":"Cell","date_updated":"2025-09-29T11:20:21Z","status":"public","author":[{"last_name":"Gadeyne","full_name":"Gadeyne, Astrid","first_name":"Astrid"},{"full_name":"Sánchez Rodríguez, Clara","last_name":"Sánchez Rodríguez","first_name":"Clara"},{"first_name":"Steffen","full_name":"Vanneste, Steffen","last_name":"Vanneste"},{"first_name":"Simone","last_name":"Di Rubbo","full_name":"Di Rubbo, Simone"},{"full_name":"Zauber, Henrik","last_name":"Zauber","first_name":"Henrik"},{"full_name":"Vanneste, Kevin","last_name":"Vanneste","first_name":"Kevin"},{"last_name":"Van Leene","full_name":"Van Leene, Jelle","first_name":"Jelle"},{"first_name":"Nancy","full_name":"De Winne, Nancy","last_name":"De Winne"},{"full_name":"Eeckhout, Dominique","last_name":"Eeckhout","first_name":"Dominique"},{"first_name":"Geert","full_name":"Persiau, Geert","last_name":"Persiau"},{"first_name":"Eveline","last_name":"Van De Slijke","full_name":"Van De Slijke, Eveline"},{"full_name":"Cannoot, Bernard","last_name":"Cannoot","first_name":"Bernard"},{"last_name":"Vercruysse","full_name":"Vercruysse, Leen","first_name":"Leen"},{"first_name":"Jonathan","full_name":"Mayers, Jonathan","last_name":"Mayers"},{"full_name":"Adamowski, Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","last_name":"Adamowski","orcid":"0000-0001-6463-5257","first_name":"Maciek"},{"id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","full_name":"Kania, Urszula","last_name":"Kania","first_name":"Urszula"},{"first_name":"Matthias","last_name":"Ehrlich","full_name":"Ehrlich, Matthias"},{"full_name":"Schweighofer, Alois","last_name":"Schweighofer","first_name":"Alois"},{"first_name":"Tijs","full_name":"Ketelaar, Tijs","last_name":"Ketelaar"},{"first_name":"Steven","last_name":"Maere","full_name":"Maere, Steven"},{"first_name":"Sebastian","last_name":"Bednarek","full_name":"Bednarek, Sebastian"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Kris","last_name":"Gevaert","full_name":"Gevaert, Kris"},{"last_name":"Witters","full_name":"Witters, Erwin","first_name":"Erwin"},{"full_name":"Russinova, Eugenia","last_name":"Russinova","first_name":"Eugenia"},{"full_name":"Persson, Staffan","last_name":"Persson","first_name":"Staffan"},{"first_name":"Geert","last_name":"De Jaeger","full_name":"De Jaeger, Geert"},{"full_name":"Van Damme, Daniël","last_name":"Van Damme","first_name":"Daniël"}],"language":[{"iso":"eng"}],"day":"13","external_id":{"isi":["000331379800009"]},"abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis."}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"4","publisher":"Cell Press","scopus_import":"1","_id":"2240","department":[{"_id":"JiFr"}],"citation":{"ieee":"A. Gadeyne <i>et al.</i>, “The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants,” <i>Cell</i>, vol. 156, no. 4. Cell Press, pp. 691–704, 2014.","mla":"Gadeyne, Astrid, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>, vol. 156, no. 4, Cell Press, 2014, pp. 691–704, doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>.","ama":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, et al. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. 2014;156(4):691-704. doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>","apa":"Gadeyne, A., Sánchez Rodríguez, C., Vanneste, S., Di Rubbo, S., Zauber, H., Vanneste, K., … Van Damme, D. (2014). The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>","short":"A. Gadeyne, C. Sánchez Rodríguez, S. Vanneste, S. Di Rubbo, H. Zauber, K. Vanneste, J. Van Leene, N. De Winne, D. Eeckhout, G. Persiau, E. Van De Slijke, B. Cannoot, L. Vercruysse, J. Mayers, M. Adamowski, U. Kania, M. Ehrlich, A. Schweighofer, T. Ketelaar, S. Maere, S. Bednarek, J. Friml, K. Gevaert, E. Witters, E. Russinova, S. Persson, G. De Jaeger, D. Van Damme, Cell 156 (2014) 691–704.","ista":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, Di Rubbo S, Zauber H, Vanneste K, Van Leene J, De Winne N, Eeckhout D, Persiau G, Van De Slijke E, Cannoot B, Vercruysse L, Mayers J, Adamowski M, Kania U, Ehrlich M, Schweighofer A, Ketelaar T, Maere S, Bednarek S, Friml J, Gevaert K, Witters E, Russinova E, Persson S, De Jaeger G, Van Damme D. 2014. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. Cell. 156(4), 691–704.","chicago":"Gadeyne, Astrid, Clara Sánchez Rodríguez, Steffen Vanneste, Simone Di Rubbo, Henrik Zauber, Kevin Vanneste, Jelle Van Leene, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>."}},{"title":"Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters","article_processing_charge":"No","month":"01","oa_version":"None","quality_controlled":"1","page":"255 - 264","date_published":"2014-01-01T00:00:00Z","doi":"10.1007/978-1-62703-592-7_23","publication_status":"published","publication":"Plant Chemical Genomics","publication_identifier":{"issn":["1064-3745"]},"publist_id":"4704","date_created":"2018-12-11T11:56:32Z","fulldoi":"https://doi.org/10.1007/978-1-62703-592-7_23","date_updated":"2025-07-10T11:52:16Z","type":"book_chapter","year":"2014","volume":1056,"intvolume":"      1056","abstract":[{"text":"Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application. ","lang":"eng"}],"editor":[{"first_name":"Glenn","full_name":"Hicks, Glenn","last_name":"Hicks"},{"first_name":"Stéphanie","full_name":"Robert, Stéphanie","last_name":"Robert"}],"language":[{"iso":"eng"}],"author":[{"orcid":"0000-0002-1998-6741","first_name":"Sibu","last_name":"Simon","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","full_name":"Simon, Sibu"},{"first_name":"Petr","last_name":"Skůpa","full_name":"Skůpa, Petr"},{"last_name":"Dobrev","full_name":"Dobrev, Petre","first_name":"Petre"},{"first_name":"Jan","full_name":"Petrášek, Jan","last_name":"Petrášek"},{"last_name":"Zažímalová","full_name":"Zažímalová, Eva","first_name":"Eva"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml","first_name":"Jirí","orcid":"0000-0002-8302-7596"}],"day":"01","status":"public","department":[{"_id":"JiFr"}],"_id":"2245","scopus_import":"1","citation":{"chicago":"Simon, Sibu, Petr Skůpa, Petre Dobrev, Jan Petrášek, Eva Zažímalová, and Jiří Friml. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” In <i>Plant Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, 1056:255–64. Methods in Molecular Biology. Springer, 2014. <a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">https://doi.org/10.1007/978-1-62703-592-7_23</a>.","ista":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. 2014.Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Plant Chemical Genomics. Methods in Molecular Biology, vol. 1056, 255–264.","short":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, J. Friml, in:, G. Hicks, S. Robert (Eds.), Plant Chemical Genomics, Springer, 2014, pp. 255–264.","apa":"Simon, S., Skůpa, P., Dobrev, P., Petrášek, J., Zažímalová, E., &#38; Friml, J. (2014). Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In G. Hicks &#38; S. Robert (Eds.), <i>Plant Chemical Genomics</i> (Vol. 1056, pp. 255–264). Springer. <a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">https://doi.org/10.1007/978-1-62703-592-7_23</a>","ama":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Hicks G, Robert S, eds. <i>Plant Chemical Genomics</i>. Vol 1056. Methods in Molecular Biology. Springer; 2014:255-264. doi:<a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">10.1007/978-1-62703-592-7_23</a>","ieee":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, and J. Friml, “Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters,” in <i>Plant Chemical Genomics</i>, vol. 1056, G. Hicks and S. Robert, Eds. Springer, 2014, pp. 255–264.","mla":"Simon, Sibu, et al. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” <i>Plant Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, vol. 1056, Springer, 2014, pp. 255–64, doi:<a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">10.1007/978-1-62703-592-7_23</a>."},"series_title":"Methods in Molecular Biology","publisher":"Springer","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","alternative_title":["Methods in Molecular Biology"]},{"doi":"10.1111/tpj.12373","date_published":"2014-01-01T00:00:00Z","page":"97 - 107","quality_controlled":"1","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981873/"}],"oa_version":"Submitted Version","oa":1,"month":"01","article_processing_charge":"No","title":"Inter-regulation of the unfolded protein response and auxin signaling","intvolume":"        77","volume":77,"isi":1,"year":"2014","type":"journal_article","date_updated":"2026-04-16T10:08:30Z","date_created":"2018-12-11T11:56:34Z","fulldoi":"https://doi.org/10.1111/tpj.12373","publist_id":"4699","publication_identifier":{"issn":["0960-7412"]},"publication_status":"published","publication":"Plant Journal","status":"public","day":"01","author":[{"full_name":"Chen, Yani","last_name":"Chen","first_name":"Yani"},{"first_name":"Kyaw","full_name":"Aung, Kyaw","last_name":"Aung"},{"full_name":"Rolčík, Jakub","last_name":"Rolčík","first_name":"Jakub"},{"first_name":"Kathryn","full_name":"Walicki, Kathryn","last_name":"Walicki"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml"},{"full_name":"Brandizzí, Federica","last_name":"Brandizzí","first_name":"Federica"}],"language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"The unfolded protein response (UPR) is a signaling network triggered by overload of protein-folding demand in the endoplasmic reticulum (ER), a condition termed ER stress. The UPR is critical for growth and development; nonetheless, connections between the UPR and other cellular regulatory processes remain largely unknown. Here, we identify a link between the UPR and the phytohormone auxin, a master regulator of plant physiology. We show that ER stress triggers down-regulation of auxin receptors and transporters in Arabidopsis thaliana. We also demonstrate that an Arabidopsis mutant of a conserved ER stress sensor IRE1 exhibits defects in the auxin response and levels. These data not only support that the plant IRE1 is required for auxin homeostasis, they also reveal a species-specific feature of IRE1 in multicellular eukaryotes. Furthermore, by establishing that UPR activation is reduced in mutants of ER-localized auxin transporters, including PIN5, we define a long-neglected biological significance of ER-based auxin regulation. We further examine the functional relationship of IRE1 and PIN5 by showing that an ire1 pin5 triple mutant enhances defects of UPR activation and auxin homeostasis in ire1 or pin5. Our results imply that the plant UPR has evolved a hormone-dependent strategy for coordinating ER function with physiological processes."}],"external_id":{"isi":["000328661300008"]},"issue":"1","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publisher":"Wiley-Blackwell","citation":{"ama":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. 2014;77(1):97-107. doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>","mla":"Chen, Yani, et al. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 97–107, doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>.","ieee":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, and F. Brandizzí, “Inter-regulation of the unfolded protein response and auxin signaling,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 97–107, 2014.","short":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, F. Brandizzí, Plant Journal 77 (2014) 97–107.","apa":"Chen, Y., Aung, K., Rolčík, J., Walicki, K., Friml, J., &#38; Brandizzí, F. (2014). Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>","ista":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. 2014. Inter-regulation of the unfolded protein response and auxin signaling. Plant Journal. 77(1), 97–107.","chicago":"Chen, Yani, Kyaw Aung, Jakub Rolčík, Kathryn Walicki, Jiří Friml, and Federica Brandizzí. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>."},"scopus_import":"1","_id":"2249","department":[{"_id":"JiFr"}]},{"date_published":"2014-01-01T00:00:00Z","doi":"10.1111/tpj.12369","article_type":"original","oa_version":"Published Version","ddc":["580"],"quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/tpj.12369"}],"page":"108 - 118","month":"01","oa":1,"title":"Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth","article_processing_charge":"No","intvolume":"        77","volume":77,"isi":1,"type":"journal_article","year":"2014","publication_status":"published","publication":"Plant Journal","publist_id":"4694","publication_identifier":{"issn":["0960-7412"]},"date_created":"2018-12-11T11:56:35Z","fulldoi":"https://doi.org/10.1111/tpj.12369","date_updated":"2026-06-18T18:33:25Z","status":"public","project":[{"_id":"256BDAB0-B435-11E9-9278-68D0E5697425","name":"Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur"}],"language":[{"iso":"eng"}],"author":[{"full_name":"Bailly, Aurélien","last_name":"Bailly","first_name":"Aurélien"},{"full_name":"Wang, Bangjun","last_name":"Wang","first_name":"Bangjun"},{"first_name":"Marta","full_name":"Zwiewka, Marta","last_name":"Zwiewka"},{"first_name":"Stephan","last_name":"Pollmann","full_name":"Pollmann, Stephan"},{"full_name":"Schenck, Daniel","last_name":"Schenck","first_name":"Daniel"},{"first_name":"Hartwig","last_name":"Lüthen","full_name":"Lüthen, Hartwig"},{"first_name":"Alexander","full_name":"Schulz, Alexander","last_name":"Schulz"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jirí"},{"first_name":"Markus","full_name":"Geisler, Markus","last_name":"Geisler"}],"day":"01","abstract":[{"text":"Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor- glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct. As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation.","lang":"eng"}],"external_id":{"isi":["000328661300009"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"1","publisher":"Wiley-Blackwell","_id":"2253","scopus_import":"1","department":[{"_id":"JiFr"}],"citation":{"ista":"Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M. 2014. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. Plant Journal. 77(1), 108–118.","chicago":"Bailly, Aurélien, Bangjun Wang, Marta Zwiewka, Stephan Pollmann, Daniel Schenck, Hartwig Lüthen, Alexander Schulz, Jiří Friml, and Markus Geisler. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>.","mla":"Bailly, Aurélien, et al. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 108–18, doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>.","ieee":"A. Bailly <i>et al.</i>, “Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 108–118, 2014.","ama":"Bailly A, Wang B, Zwiewka M, et al. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. 2014;77(1):108-118. doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>","short":"A. Bailly, B. Wang, M. Zwiewka, S. Pollmann, D. Schenck, H. Lüthen, A. Schulz, J. Friml, M. Geisler, Plant Journal 77 (2014) 108–118.","apa":"Bailly, A., Wang, B., Zwiewka, M., Pollmann, S., Schenck, D., Lüthen, H., … Geisler, M. (2014). Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>"}},{"intvolume":"         4","isi":1,"volume":4,"type":"journal_article","year":"2014","date_updated":"2025-09-29T11:32:17Z","publist_id":"4786","publication_status":"published","publication":"Open Biology","fulldoi":"https://doi.org/10.1098/rsob.140017","date_created":"2018-12-11T11:56:13Z","file":[{"relation":"main_file","creator":"system","content_type":"application/pdf","date_created":"2018-12-12T10:13:40Z","date_updated":"2020-07-14T12:45:31Z","file_name":"IST-2016-441-v1+1_140017.full.pdf","file_id":"5025","file_size":682570,"checksum":"2020627feff36cf0799167c84149fa75","access_level":"open_access"}],"doi":"10.1098/rsob.140017","date_published":"2014-04-16T00:00:00Z","corr_author":"1","has_accepted_license":"1","oa_version":"Published Version","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"quality_controlled":"1","license":"https://creativecommons.org/licenses/by/4.0/","oa":1,"month":"04","article_processing_charge":"No","title":"Polar delivery in plants; commonalities and differences to animal epithelial cells","issue":"APRIL","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_number":"140017","publisher":"Royal Society","citation":{"chicago":"Kania, Urszula, Matyas Fendrych, and Jiří Friml. “Polar Delivery in Plants; Commonalities and Differences to Animal Epithelial Cells.” <i>Open Biology</i>. Royal Society, 2014. <a href=\"https://doi.org/10.1098/rsob.140017\">https://doi.org/10.1098/rsob.140017</a>.","ista":"Kania U, Fendrych M, Friml J. 2014. Polar delivery in plants; commonalities and differences to animal epithelial cells. Open Biology. 4(APRIL), 140017.","apa":"Kania, U., Fendrych, M., &#38; Friml, J. (2014). Polar delivery in plants; commonalities and differences to animal epithelial cells. <i>Open Biology</i>. Royal Society. <a href=\"https://doi.org/10.1098/rsob.140017\">https://doi.org/10.1098/rsob.140017</a>","short":"U. Kania, M. Fendrych, J. Friml, Open Biology 4 (2014).","ama":"Kania U, Fendrych M, Friml J. Polar delivery in plants; commonalities and differences to animal epithelial cells. <i>Open Biology</i>. 2014;4(APRIL). doi:<a href=\"https://doi.org/10.1098/rsob.140017\">10.1098/rsob.140017</a>","mla":"Kania, Urszula, et al. “Polar Delivery in Plants; Commonalities and Differences to Animal Epithelial Cells.” <i>Open Biology</i>, vol. 4, no. APRIL, 140017, Royal Society, 2014, doi:<a href=\"https://doi.org/10.1098/rsob.140017\">10.1098/rsob.140017</a>.","ieee":"U. Kania, M. Fendrych, and J. Friml, “Polar delivery in plants; commonalities and differences to animal epithelial cells,” <i>Open Biology</i>, vol. 4, no. APRIL. Royal Society, 2014."},"acknowledgement":"This work was supported by a grant from the Research Foundation-Flanders (Odysseus).\r\n\r\n","scopus_import":"1","_id":"2188","department":[{"_id":"JiFr"}],"status":"public","pubrep_id":"441","file_date_updated":"2020-07-14T12:45:31Z","day":"16","language":[{"iso":"eng"}],"author":[{"full_name":"Kania, Urszula","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","last_name":"Kania","first_name":"Urszula"},{"full_name":"Fendrych, Matyas","last_name":"Fendrych","first_name":"Matyas"},{"orcid":"0000-0002-8302-7596","first_name":"Jiřĺ","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiřĺ","last_name":"Friml"}],"abstract":[{"lang":"eng","text":"Although plant and animal cells use a similar core mechanism to deliver proteins to the plasma membrane, their different lifestyle, body organization and specific cell structures resulted in the acquisition of regulatory mechanisms that vary in the two kingdoms. In particular, cell polarity regulators do not seem to be conserved, because genes encoding key components are absent in plant genomes. In plants, the broad knowledge on polarity derives from the study of auxin transporters, the PIN-FORMED proteins, in the model plant Arabidopsis thaliana. In animals, much information is provided from the study of polarity in epithelial cells that exhibit basolateral and luminal apical polarities, separated by tight junctions. In this review, we summarize the similarities and differences of the polarization mechanisms between plants and animals and survey the main genetic approaches that have been used to characterize new genes involved in polarity establishment in plants, including the frequently used forward and reverse genetics screens as well as a novel chemical genetics approach that is expected to overcome the limitation of classical genetics methods."}],"external_id":{"isi":["000335813800006"]}},{"abstract":[{"lang":"eng","text":"To control morphogenesis, molecular regulatory networks have to interfere with the mechanical properties of the individual cells of developing organs and tissues, but how this is achieved is not well known. We study this issue here in the shoot meristem of higher plants, a group of undifferentiated cells where complex changes in growth rates and directions lead to the continuous formation of new organs [1, 2]. Here, we show that the plant hormone auxin plays an important role in this process via a dual, local effect on the extracellular matrix, the cell wall, which determines cell shape. Our study reveals that auxin not only causes a limited reduction in wall stiffness but also directly interferes with wall anisotropy via the regulation of cortical microtubule dynamics. We further show that to induce growth isotropy and organ outgrowth, auxin somehow interferes with the cortical microtubule-ordering activity of a network of proteins, including AUXIN BINDING PROTEIN 1 and KATANIN 1. Numerical simulations further indicate that the induced isotropy is sufficient to amplify the effects of the relatively minor changes in wall stiffness to promote organogenesis and the establishment of new growth axes in a robust manner."}],"external_id":{"isi":["000342747600031"]},"language":[{"iso":"eng"}],"author":[{"full_name":"Sassi, Massimiliano","last_name":"Sassi","first_name":"Massimiliano"},{"last_name":"Ali","full_name":"Ali, Olivier","first_name":"Olivier"},{"first_name":"Frédéric","last_name":"Boudon","full_name":"Boudon, Frédéric"},{"last_name":"Cloarec","full_name":"Cloarec, Gladys","first_name":"Gladys"},{"last_name":"Abad","full_name":"Abad, Ursula","first_name":"Ursula"},{"first_name":"Coralie","last_name":"Cellier","full_name":"Cellier, Coralie"},{"full_name":"Chen, Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","last_name":"Chen","first_name":"Xu"},{"first_name":"Benjamin","full_name":"Gilles, Benjamin","last_name":"Gilles"},{"full_name":"Milani, Pascale","last_name":"Milani","first_name":"Pascale"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Teva","full_name":"Vernoux, Teva","last_name":"Vernoux"},{"first_name":"Christophe","full_name":"Godin, Christophe","last_name":"Godin"},{"full_name":"Hamant, Olivier","last_name":"Hamant","first_name":"Olivier"},{"last_name":"Traas","full_name":"Traas, Jan","first_name":"Jan"}],"day":"06","status":"public","department":[{"_id":"JiFr"}],"_id":"1852","scopus_import":"1","citation":{"ista":"Sassi M, Ali O, Boudon F, Cloarec G, Abad U, Cellier C, Chen X, Gilles B, Milani P, Friml J, Vernoux T, Godin C, Hamant O, Traas J. 2014. An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. Current Biology. 24(19), 2335–2342.","chicago":"Sassi, Massimiliano, Olivier Ali, Frédéric Boudon, Gladys Cloarec, Ursula Abad, Coralie Cellier, Xu Chen, et al. “An Auxin-Mediated Shift toward Growth Isotropy Promotes Organ Formation at the Shoot Meristem in Arabidopsis.” <i>Current Biology</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">https://doi.org/10.1016/j.cub.2014.08.036</a>.","ieee":"M. Sassi <i>et al.</i>, “An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis,” <i>Current Biology</i>, vol. 24, no. 19. Cell Press, pp. 2335–2342, 2014.","mla":"Sassi, Massimiliano, et al. “An Auxin-Mediated Shift toward Growth Isotropy Promotes Organ Formation at the Shoot Meristem in Arabidopsis.” <i>Current Biology</i>, vol. 24, no. 19, Cell Press, 2014, pp. 2335–42, doi:<a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">10.1016/j.cub.2014.08.036</a>.","ama":"Sassi M, Ali O, Boudon F, et al. An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. <i>Current Biology</i>. 2014;24(19):2335-2342. doi:<a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">10.1016/j.cub.2014.08.036</a>","short":"M. Sassi, O. Ali, F. Boudon, G. Cloarec, U. Abad, C. Cellier, X. Chen, B. Gilles, P. Milani, J. Friml, T. Vernoux, C. Godin, O. Hamant, J. Traas, Current Biology 24 (2014) 2335–2342.","apa":"Sassi, M., Ali, O., Boudon, F., Cloarec, G., Abad, U., Cellier, C., … Traas, J. (2014). An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">https://doi.org/10.1016/j.cub.2014.08.036</a>"},"acknowledgement":"This work was funded by grants from EraSysBio+ (iSAM) and ERC (Morphodynamics). ","publisher":"Cell Press","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"19","title":"An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis","article_processing_charge":"No","month":"10","oa":1,"oa_version":"Submitted Version","main_file_link":[{"open_access":"1","url":"https://hal.archives-ouvertes.fr/hal-01074821"}],"quality_controlled":"1","page":"2335 - 2342","date_published":"2014-10-06T00:00:00Z","doi":"10.1016/j.cub.2014.08.036","publication_status":"published","publication":"Current Biology","publist_id":"5248","date_created":"2018-12-11T11:54:22Z","fulldoi":"https://doi.org/10.1016/j.cub.2014.08.036","date_updated":"2025-09-29T13:10:45Z","type":"journal_article","year":"2014","isi":1,"volume":24,"intvolume":"        24"},{"article_processing_charge":"No","title":"Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules","oa":1,"month":"12","page":"90 - 93","corr_author":"1","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4257754/"}],"pmid":1,"quality_controlled":"1","oa_version":"Submitted Version","article_type":"original","doi":"10.1038/nature13889","date_published":"2014-12-04T00:00:00Z","date_updated":"2025-09-29T13:10:05Z","fulldoi":"https://doi.org/10.1038/nature13889","date_created":"2018-12-11T11:54:25Z","publist_id":"5237","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"publication_status":"published","publication":"Nature","year":"2014","type":"journal_article","isi":1,"volume":516,"intvolume":"       516","external_id":{"isi":["000346310800045"],"pmid":["25409144"]},"abstract":[{"lang":"eng","text":"The prominent and evolutionarily ancient role of the plant hormone auxin is the regulation of cell expansion. Cell expansion requires ordered arrangement of the cytoskeleton but molecular mechanisms underlying its regulation by signalling molecules including auxin are unknown. Here we show in the model plant Arabidopsis thaliana that in elongating cells exogenous application of auxin or redistribution of endogenous auxin induces very rapid microtubule re-orientation from transverse to longitudinal, coherent with the inhibition of cell expansion. This fast auxin effect requires auxin binding protein 1 (ABP1) and involves a contribution of downstream signalling components such as ROP6 GTPase, ROP-interactive protein RIC1 and the microtubule-severing protein katanin. These components are required for rapid auxin-and ABP1-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses."}],"day":"04","author":[{"last_name":"Chen","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","full_name":"Chen, Xu","first_name":"Xu"},{"full_name":"Grandont, Laurie","last_name":"Grandont","first_name":"Laurie"},{"last_name":"Li","full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","first_name":"Hongjiang","orcid":"0000-0001-5039-9660"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","last_name":"Hauschild","orcid":"0000-0001-9843-3522","first_name":"Robert"},{"first_name":"Sébastien","last_name":"Paque","full_name":"Paque, Sébastien"},{"first_name":"Anas","last_name":"Abuzeineh","full_name":"Abuzeineh, Anas"},{"first_name":"Hana","id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA","full_name":"Rakusova, Hana","last_name":"Rakusova"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"},{"full_name":"Perrot Rechenmann, Catherine","last_name":"Perrot Rechenmann","first_name":"Catherine"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"language":[{"iso":"eng"}],"status":"public","project":[{"grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants"}],"acknowledgement":"We thank R. Dixit for performing complementary experiments, D. W. Ehrhardt and T. Hashimoto for providing the seeds of TUB6–RFP and EB1b–GFP respectively, E. Zazimalova, J. Petrasek and M. Fendrych for discussing the manuscript and J. Leung for text optimization. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP, to J.F.), ANR blanc AuxiWall project (ANR-11-BSV5-0007, to C.P.-R. and L.G.) and the Agency for Innovation by Science and Technology (IWT) (to H.R.). This work benefited from the facilities and expertise of the Imagif Cell Biology platform (http://www.imagif.cnrs.fr), which is supported by the Conseil Général de l’Essonne.","citation":{"apa":"Chen, X., Grandont, L., Li, H., Hauschild, R., Paque, S., Abuzeineh, A., … Friml, J. (2014). Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature13889\">https://doi.org/10.1038/nature13889</a>","short":"X. Chen, L. Grandont, H. Li, R. Hauschild, S. Paque, A. Abuzeineh, H. Rakusova, E. Benková, C. Perrot Rechenmann, J. Friml, Nature 516 (2014) 90–93.","ama":"Chen X, Grandont L, Li H, et al. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. <i>Nature</i>. 2014;516(729):90-93. doi:<a href=\"https://doi.org/10.1038/nature13889\">10.1038/nature13889</a>","mla":"Chen, Xu, et al. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” <i>Nature</i>, vol. 516, no. 729, Nature Publishing Group, 2014, pp. 90–93, doi:<a href=\"https://doi.org/10.1038/nature13889\">10.1038/nature13889</a>.","ieee":"X. Chen <i>et al.</i>, “Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules,” <i>Nature</i>, vol. 516, no. 729. Nature Publishing Group, pp. 90–93, 2014.","chicago":"Chen, Xu, Laurie Grandont, Hongjiang Li, Robert Hauschild, Sébastien Paque, Anas Abuzeineh, Hana Rakusova, Eva Benková, Catherine Perrot Rechenmann, and Jiří Friml. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” <i>Nature</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/nature13889\">https://doi.org/10.1038/nature13889</a>.","ista":"Chen X, Grandont L, Li H, Hauschild R, Paque S, Abuzeineh A, Rakusova H, Benková E, Perrot Rechenmann C, Friml J. 2014. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. Nature. 516(729), 90–93."},"department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"EvBe"}],"_id":"1862","scopus_import":"1","publisher":"Nature Publishing Group","issue":"729","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","ec_funded":1},{"status":"public","project":[{"grant_number":"282300","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"language":[{"iso":"eng"}],"author":[{"first_name":"Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87","full_name":"Nováková, Petra","last_name":"Nováková"},{"last_name":"Hirsch","full_name":"Hirsch, Sibylle","first_name":"Sibylle"},{"full_name":"Feraru, Elena","last_name":"Feraru","first_name":"Elena"},{"first_name":"Ricardo","full_name":"Tejos, Ricardo","last_name":"Tejos"},{"last_name":"Van Wijk","full_name":"Van Wijk, Ringo","first_name":"Ringo"},{"first_name":"Tom","full_name":"Viaene, Tom","last_name":"Viaene"},{"full_name":"Heilmann, Mareike","last_name":"Heilmann","first_name":"Mareike"},{"full_name":"Lerche, Jennifer","last_name":"Lerche","first_name":"Jennifer"},{"first_name":"Riet","full_name":"De Rycke, Riet","last_name":"De Rycke"},{"last_name":"Feraru","full_name":"Feraru, Mugurel","first_name":"Mugurel"},{"first_name":"Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87","full_name":"Grones, Peter","last_name":"Grones"},{"first_name":"Marc","last_name":"Van Montagu","full_name":"Van Montagu, Marc"},{"first_name":"Ingo","full_name":"Heilmann, Ingo","last_name":"Heilmann"},{"full_name":"Munnik, Teun","last_name":"Munnik","first_name":"Teun"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","orcid":"0000-0002-8302-7596"}],"day":"18","external_id":{"isi":["000331396500082"]},"abstract":[{"lang":"eng","text":"Phosphatidylinositol (PtdIns) is a structural phospholipid that can be phosphorylated into various lipid signaling molecules, designated polyphosphoinositides (PPIs). The reversible phosphorylation of PPIs on the 3, 4, or 5 position of inositol is performed by a set of organelle-specific kinases and phosphatases, and the characteristic head groups make these molecules ideal for regulating biological processes in time and space. In yeast and mammals, PtdIns3P and PtdIns(3,5)P2 play crucial roles in trafficking toward the lytic compartments, whereas the role in plants is not yet fully understood. Here we identified the role of a land plant-specific subgroup of PPI phosphatases, the suppressor of actin 2 (SAC2) to SAC5, during vacuolar trafficking and morphogenesis in Arabidopsis thaliana. SAC2-SAC5 localize to the tonoplast along with PtdIns3P, the presumable product of their activity. In SAC gain- and loss-of-function mutants, the levels of PtdIns monophosphates and bisphosphates were changed, with opposite effects on the morphology of storage and lytic vacuoles, and the trafficking toward the vacuoles was defective. Moreover, multiple sac knockout mutants had an increased number of smaller storage and lytic vacuoles, whereas extralarge vacuoles were observed in the overexpression lines, correlating with various growth and developmental defects. The fragmented vacuolar phenotype of sac mutants could be mimicked by treating wild-type seedlings with PtdIns(3,5)P2, corroborating that this PPI is important for vacuole morphology. Taken together, these results provide evidence that PPIs, together with their metabolic enzymes SAC2-SAC5, are crucial for vacuolar trafficking and for vacuolar morphology and function in plants."}],"ec_funded":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"7","publisher":"National Academy of Sciences","_id":"1893","scopus_import":"1","department":[{"_id":"JiFr"}],"citation":{"ama":"Marhavá P, Hirsch S, Feraru E, et al. SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis. <i>PNAS</i>. 2014;111(7):2818-2823. doi:<a href=\"https://doi.org/10.1073/pnas.1324264111\">10.1073/pnas.1324264111</a>","ieee":"P. Marhavá <i>et al.</i>, “SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis,” <i>PNAS</i>, vol. 111, no. 7. National Academy of Sciences, pp. 2818–2823, 2014.","mla":"Marhavá, Petra, et al. “SAC Phosphoinositide Phosphatases at the Tonoplast Mediate Vacuolar Function in Arabidopsis.” <i>PNAS</i>, vol. 111, no. 7, National Academy of Sciences, 2014, pp. 2818–23, doi:<a href=\"https://doi.org/10.1073/pnas.1324264111\">10.1073/pnas.1324264111</a>.","short":"P. Marhavá, S. Hirsch, E. Feraru, R. Tejos, R. Van Wijk, T. Viaene, M. Heilmann, J. Lerche, R. De Rycke, M. Feraru, P. Grones, M. Van Montagu, I. Heilmann, T. Munnik, J. Friml, PNAS 111 (2014) 2818–2823.","apa":"Marhavá, P., Hirsch, S., Feraru, E., Tejos, R., Van Wijk, R., Viaene, T., … Friml, J. (2014). SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1324264111\">https://doi.org/10.1073/pnas.1324264111</a>","ista":"Marhavá P, Hirsch S, Feraru E, Tejos R, Van Wijk R, Viaene T, Heilmann M, Lerche J, De Rycke R, Feraru M, Grones P, Van Montagu M, Heilmann I, Munnik T, Friml J. 2014. SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis. PNAS. 111(7), 2818–2823.","chicago":"Marhavá, Petra, Sibylle Hirsch, Elena Feraru, Ricardo Tejos, Ringo Van Wijk, Tom Viaene, Mareike Heilmann, et al. “SAC Phosphoinositide Phosphatases at the Tonoplast Mediate Vacuolar Function in Arabidopsis.” <i>PNAS</i>. National Academy of Sciences, 2014. <a href=\"https://doi.org/10.1073/pnas.1324264111\">https://doi.org/10.1073/pnas.1324264111</a>."},"acknowledgement":"This work was supported by grants from the Research Foundation-Flanders (Odysseus).","date_published":"2014-02-18T00:00:00Z","doi":"10.1073/pnas.1324264111","oa_version":"Submitted Version","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932866/","open_access":"1"}],"corr_author":"1","page":"2818 - 2823","month":"02","oa":1,"title":"SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis","article_processing_charge":"No","intvolume":"       111","isi":1,"volume":111,"type":"journal_article","year":"2014","publication_status":"published","publist_id":"5202","publication":"PNAS","fulldoi":"https://doi.org/10.1073/pnas.1324264111","date_created":"2018-12-11T11:54:34Z","date_updated":"2025-09-29T13:05:44Z"},{"intvolume":"        26","isi":1,"volume":26,"year":"2014","type":"journal_article","date_created":"2018-12-11T11:54:36Z","fulldoi":"https://doi.org/10.1105/tpc.114.125880","publist_id":"5199","publication":"Plant Cell","publication_status":"published","date_updated":"2025-09-29T13:04:06Z","date_published":"2014-07-01T00:00:00Z","doi":"10.1105/tpc.114.125880","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145132/","open_access":"1"}],"oa_version":"Submitted Version","page":"3062 - 3076","month":"07","oa":1,"title":"Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis","article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"7","publisher":"American Society of Plant Biologists","_id":"1897","department":[{"_id":"JiFr"}],"scopus_import":"1","acknowledgement":"This work was supported by the Odysseus Program of the Research Foundation-Flanders (J.F.).","citation":{"apa":"Naramoto, S., Otegui, M., Kutsuna, N., De Rycke, R., Dainobu, T., Karampelias, M., … Friml, J. (2014). Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.114.125880\">https://doi.org/10.1105/tpc.114.125880</a>","short":"S. Naramoto, M. Otegui, N. Kutsuna, R. De Rycke, T. Dainobu, M. Karampelias, M. Fujimoto, E. Feraru, D. Miki, H. Fukuda, A. Nakano, J. Friml, Plant Cell 26 (2014) 3062–3076.","ama":"Naramoto S, Otegui M, Kutsuna N, et al. Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis. <i>Plant Cell</i>. 2014;26(7):3062-3076. doi:<a href=\"https://doi.org/10.1105/tpc.114.125880\">10.1105/tpc.114.125880</a>","ieee":"S. Naramoto <i>et al.</i>, “Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis,” <i>Plant Cell</i>, vol. 26, no. 7. American Society of Plant Biologists, pp. 3062–3076, 2014.","mla":"Naramoto, Satoshi, et al. “Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis.” <i>Plant Cell</i>, vol. 26, no. 7, American Society of Plant Biologists, 2014, pp. 3062–76, doi:<a href=\"https://doi.org/10.1105/tpc.114.125880\">10.1105/tpc.114.125880</a>.","chicago":"Naramoto, Satoshi, Marisa Otegui, Natsumaro Kutsuna, Riet De Rycke, Tomoko Dainobu, Michael Karampelias, Masaru Fujimoto, et al. “Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis.” <i>Plant Cell</i>. American Society of Plant Biologists, 2014. <a href=\"https://doi.org/10.1105/tpc.114.125880\">https://doi.org/10.1105/tpc.114.125880</a>.","ista":"Naramoto S, Otegui M, Kutsuna N, De Rycke R, Dainobu T, Karampelias M, Fujimoto M, Feraru E, Miki D, Fukuda H, Nakano A, Friml J. 2014. Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis. Plant Cell. 26(7), 3062–3076."},"status":"public","author":[{"full_name":"Naramoto, Satoshi","last_name":"Naramoto","first_name":"Satoshi"},{"first_name":"Marisa","full_name":"Otegui, Marisa","last_name":"Otegui"},{"full_name":"Kutsuna, Natsumaro","last_name":"Kutsuna","first_name":"Natsumaro"},{"full_name":"De Rycke, Riet","last_name":"De Rycke","first_name":"Riet"},{"first_name":"Tomoko","last_name":"Dainobu","full_name":"Dainobu, Tomoko"},{"full_name":"Karampelias, Michael","last_name":"Karampelias","first_name":"Michael"},{"full_name":"Fujimoto, Masaru","last_name":"Fujimoto","first_name":"Masaru"},{"last_name":"Feraru","full_name":"Feraru, Elena","first_name":"Elena"},{"first_name":"Daisuke","last_name":"Miki","full_name":"Miki, Daisuke"},{"first_name":"Hiroo","full_name":"Fukuda, Hiroo","last_name":"Fukuda"},{"full_name":"Nakano, Akihiko","last_name":"Nakano","first_name":"Akihiko"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml"}],"language":[{"iso":"eng"}],"day":"01","external_id":{"isi":["000342076200027"]},"abstract":[{"text":"GNOM is one of the most characterized membrane trafficking regulators in plants, with crucial roles in development. GNOM encodes an ARF-guanine nucleotide exchange factor (ARF-GEF) that activates small GTPases of the ARF (ADP ribosylation factor) class to mediate vesicle budding at endomembranes. The crucial role of GNOM in recycling of PIN auxin transporters and other proteins to the plasma membrane was identified in studies using the ARF-GEF inhibitor brefeldin A (BFA). GNOM, the most prominent regulator of recycling in plants, has been proposed to act and localize at so far elusive recycling endosomes. Here, we report the GNOM localization in context of its cellular function in Arabidopsis thaliana. State-of-the-art imaging, pharmacological interference, and ultrastructure analysis show that GNOM predominantly localizes to Golgi apparatus. Super-resolution confocal live imaging microscopy identified GNOM and its closest homolog GNOM-like 1 at distinct subdomains on Golgi cisternae. Short-term BFA treatment stabilizes GNOM at the Golgi apparatus, whereas prolonged exposures results in GNOM translocation to trans-Golgi network (TGN)/early endosomes (EEs). Malformed TGN/EE in gnom mutants suggests a role for GNOM in maintaining TGN/EE function. Our results redefine the subcellular action of GNOM and reevaluate the identity and function of recycling endosomes in plants.","lang":"eng"}]},{"scopus_import":"1","department":[{"_id":"JiFr"}],"_id":"1901","acknowledgement":"This work was supported by funding from the projects CZ.1.07/2.3.00/20.0043 and CZ.1.05/1.1.00/02.0068 (to CEITEC, Central European Institute of Technology) and the Odysseus program of the Research Foundation-Flanders to J.F\r\n","citation":{"chicago":"Tian, Huiyu, Krzysztof T Wabnik, Tiantian Niu, Hongjiang Li, Qianqian Yu, Stephan Pollmann, Steffen Vanneste, et al. “WOX5-IAA17 Feedback Circuit-Mediated Cellular Auxin Response Is Crucial for the Patterning of Root Stem Cell Niches in Arabidopsis.” <i>Molecular Plant</i>. Oxford University Press, 2014. <a href=\"https://doi.org/10.1093/mp/sst118\">https://doi.org/10.1093/mp/sst118</a>.","ista":"Tian H, Wabnik KT, Niu T, Li H, Yu Q, Pollmann S, Vanneste S, Govaerts W, Rolčík J, Geisler M, Friml J, Ding Z. 2014. WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis. Molecular Plant. 7(2), 277–289.","apa":"Tian, H., Wabnik, K. T., Niu, T., Li, H., Yu, Q., Pollmann, S., … Ding, Z. (2014). WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis. <i>Molecular Plant</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/mp/sst118\">https://doi.org/10.1093/mp/sst118</a>","short":"H. Tian, K.T. Wabnik, T. Niu, H. Li, Q. Yu, S. Pollmann, S. Vanneste, W. Govaerts, J. Rolčík, M. Geisler, J. Friml, Z. Ding, Molecular Plant 7 (2014) 277–289.","ama":"Tian H, Wabnik KT, Niu T, et al. WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis. <i>Molecular Plant</i>. 2014;7(2):277-289. doi:<a href=\"https://doi.org/10.1093/mp/sst118\">10.1093/mp/sst118</a>","ieee":"H. Tian <i>et al.</i>, “WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis,” <i>Molecular Plant</i>, vol. 7, no. 2. Oxford University Press, pp. 277–289, 2014.","mla":"Tian, Huiyu, et al. “WOX5-IAA17 Feedback Circuit-Mediated Cellular Auxin Response Is Crucial for the Patterning of Root Stem Cell Niches in Arabidopsis.” <i>Molecular Plant</i>, vol. 7, no. 2, Oxford University Press, 2014, pp. 277–89, doi:<a href=\"https://doi.org/10.1093/mp/sst118\">10.1093/mp/sst118</a>."},"publisher":"Oxford University Press","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"2","abstract":[{"lang":"eng","text":"In plants, the patterning of stem cell-enriched meristems requires a graded auxin response maximum that emerges from the concerted action of polar auxin transport, auxin biosynthesis, auxin metabolism, and cellular auxin response machinery. However, mechanisms underlying this auxin response maximum-mediated root stem cell maintenance are not fully understood. Here, we present unexpected evidence that WUSCHEL-RELATED HOMEOBOX 5 (WOX5) transcription factor modulates expression of auxin biosynthetic genes in the quiescent center (QC) of the root and thus provides a robust mechanism for the maintenance of auxin response maximum in the root tip. This WOX5 action is balanced through the activity of indole-3-acetic acid 17 (IAA17) auxin response repressor. Our combined genetic, cell biology, and computational modeling studies revealed a previously uncharacterized feedback loop linking WOX5-mediated auxin production to IAA17-dependent repression of auxin responses. This WOX5-IAA17 feedback circuit further assures the maintenance of auxin response maximum in the root tip and thereby contributes to the maintenance of distal stem cell (DSC) populations. Our experimental studies and in silico computer simulations both demonstrate that the WOX5-IAA17 feedback circuit is essential for the maintenance of auxin gradient in the root tip and the auxin-mediated root DSC differentiation."}],"external_id":{"isi":["000330841400003"]},"author":[{"last_name":"Tian","full_name":"Tian, Huiyu","first_name":"Huiyu"},{"first_name":"Krzysztof T","last_name":"Wabnik","full_name":"Wabnik, Krzysztof T"},{"first_name":"Tiantian","last_name":"Niu","full_name":"Niu, Tiantian"},{"first_name":"Hongjiang","full_name":"Li, Hongjiang","last_name":"Li"},{"first_name":"Qianqian","last_name":"Yu","full_name":"Yu, Qianqian"},{"first_name":"Stephan","full_name":"Pollmann, Stephan","last_name":"Pollmann"},{"first_name":"Steffen","full_name":"Vanneste, Steffen","last_name":"Vanneste"},{"first_name":"Willy","last_name":"Govaerts","full_name":"Govaerts, Willy"},{"last_name":"Rolčík","full_name":"Rolčík, Jakub","first_name":"Jakub"},{"last_name":"Geisler","full_name":"Geisler, Markus","first_name":"Markus"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","first_name":"Jirí"},{"last_name":"Ding","full_name":"Ding, Zhaojun","first_name":"Zhaojun"}],"language":[{"iso":"eng"}],"day":"01","status":"public","date_created":"2018-12-11T11:54:37Z","fulldoi":"https://doi.org/10.1093/mp/sst118","publication":"Molecular Plant","publication_status":"published","publist_id":"5194","date_updated":"2025-09-29T12:30:56Z","year":"2014","type":"journal_article","isi":1,"volume":7,"intvolume":"         7","title":"WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis","article_processing_charge":"No","month":"02","oa_version":"None","page":"277 - 289","date_published":"2014-02-01T00:00:00Z","doi":"10.1093/mp/sst118"},{"_id":"1914","department":[{"_id":"JiFr"}],"scopus_import":"1","citation":{"ista":"Sauer M, Friml J. 2014. Plant biology: Gatekeepers of the road to protein perdition. Current Biology. 24(1), R27–R29.","chicago":"Sauer, Michael, and Jiří Friml. “Plant Biology: Gatekeepers of the Road to Protein Perdition.” <i>Current Biology</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cub.2013.11.019\">https://doi.org/10.1016/j.cub.2013.11.019</a>.","mla":"Sauer, Michael, and Jiří Friml. “Plant Biology: Gatekeepers of the Road to Protein Perdition.” <i>Current Biology</i>, vol. 24, no. 1, Cell Press, 2014, pp. R27–29, doi:<a href=\"https://doi.org/10.1016/j.cub.2013.11.019\">10.1016/j.cub.2013.11.019</a>.","ieee":"M. Sauer and J. Friml, “Plant biology: Gatekeepers of the road to protein perdition,” <i>Current Biology</i>, vol. 24, no. 1. Cell Press, pp. R27–R29, 2014.","ama":"Sauer M, Friml J. Plant biology: Gatekeepers of the road to protein perdition. <i>Current Biology</i>. 2014;24(1):R27-R29. doi:<a href=\"https://doi.org/10.1016/j.cub.2013.11.019\">10.1016/j.cub.2013.11.019</a>","short":"M. Sauer, J. Friml, Current Biology 24 (2014) R27–R29.","apa":"Sauer, M., &#38; Friml, J. (2014). Plant biology: Gatekeepers of the road to protein perdition. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2013.11.019\">https://doi.org/10.1016/j.cub.2013.11.019</a>"},"publisher":"Cell Press","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"1","abstract":[{"text":"Targeting membrane proteins for degradation requires the sequential action of ESCRT sub-complexes ESCRT-0 to ESCRT-III. Although this machinery is generally conserved among kingdoms, plants lack the essential ESCRT-0 components. A new report closes this gap by identifying a novel protein family that substitutes for ESCRT-0 function in plants.","lang":"eng"}],"external_id":{"isi":["000329501400011"]},"language":[{"iso":"eng"}],"author":[{"first_name":"Michael","last_name":"Sauer","full_name":"Sauer, Michael"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí"}],"day":"06","status":"public","publication":"Current Biology","publist_id":"5180","publication_status":"published","fulldoi":"https://doi.org/10.1016/j.cub.2013.11.019","date_created":"2018-12-11T11:54:41Z","date_updated":"2025-09-29T12:23:35Z","type":"journal_article","year":"2014","isi":1,"volume":24,"intvolume":"        24","title":"Plant biology: Gatekeepers of the road to protein perdition","article_processing_charge":"No","month":"01","oa_version":"None","quality_controlled":"1","corr_author":"1","page":"R27 - R29","date_published":"2014-01-06T00:00:00Z","doi":"10.1016/j.cub.2013.11.019"},{"date_published":"2014-02-01T00:00:00Z","doi":"10.1042/BST20130269","quality_controlled":"1","pmid":1,"oa_version":"None","article_type":"original","page":"212 - 218","corr_author":"1","month":"02","title":"Rho-GTPase-regulated vesicle trafficking in plant cell polarity","article_processing_charge":"No","intvolume":"        42","isi":1,"volume":42,"year":"2014","type":"journal_article","fulldoi":"https://doi.org/10.1042/BST20130269","date_created":"2018-12-11T11:54:41Z","publication":"Biochemical Society Transactions","publist_id":"5179","publication_status":"published","publication_identifier":{"eissn":["1470-8752"],"issn":["0300-5127"]},"date_updated":"2025-09-29T12:22:54Z","project":[{"grant_number":"282300","call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"status":"public","author":[{"first_name":"Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","full_name":"Chen, Xu","last_name":"Chen"},{"last_name":"Friml","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí"}],"language":[{"iso":"eng"}],"day":"01","external_id":{"pmid":["24450654"],"isi":["000333444400034"]},"abstract":[{"text":"ROPs (Rho of plants) belong to a large family of plant-specific Rho-like small GTPases that function as essential molecular switches to control diverse cellular processes including cytoskeleton organization, cell polarization, cytokinesis, cell differentiation and vesicle trafficking. Although the machineries of vesicle trafficking and cell polarity in plants have been individually well addressed, how ROPs co-ordinate those processes is still largely unclear. Recent progress has been made towards an understanding of the coordination of ROP signalling and trafficking of PIN (PINFORMED) transporters for the plant hormone auxin in both root and leaf pavement cells. PIN transporters constantly shuttle between the endosomal compartments and the polar plasma membrane domains, therefore the modulation of PIN-dependent auxin transport between cells is a main developmental output of ROP-regulated vesicle trafficking. The present review focuses on these cellular mechanisms, especially the integration of ROP-based vesicle trafficking and plant cell polarity.","lang":"eng"}],"ec_funded":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"1","publisher":"Portland Press","_id":"1915","department":[{"_id":"JiFr"}],"scopus_import":"1","acknowledgement":"This work was supported by the European Research Council [project ERC-2011-StG-20101109-PSDP], Central European Institute of Technology (CEITEC) [grant number CZ.1.05/1.1.00/02.0068], European Social Fund [grant number CZ.1.07/2.3.00/20.0043] and the Czec","citation":{"mla":"Chen, Xu, and Jiří Friml. “Rho-GTPase-Regulated Vesicle Trafficking in Plant Cell Polarity.” <i>Biochemical Society Transactions</i>, vol. 42, no. 1, Portland Press, 2014, pp. 212–18, doi:<a href=\"https://doi.org/10.1042/BST20130269\">10.1042/BST20130269</a>.","ieee":"X. Chen and J. Friml, “Rho-GTPase-regulated vesicle trafficking in plant cell polarity,” <i>Biochemical Society Transactions</i>, vol. 42, no. 1. Portland Press, pp. 212–218, 2014.","ama":"Chen X, Friml J. Rho-GTPase-regulated vesicle trafficking in plant cell polarity. <i>Biochemical Society Transactions</i>. 2014;42(1):212-218. doi:<a href=\"https://doi.org/10.1042/BST20130269\">10.1042/BST20130269</a>","short":"X. Chen, J. Friml, Biochemical Society Transactions 42 (2014) 212–218.","apa":"Chen, X., &#38; Friml, J. (2014). Rho-GTPase-regulated vesicle trafficking in plant cell polarity. <i>Biochemical Society Transactions</i>. Portland Press. <a href=\"https://doi.org/10.1042/BST20130269\">https://doi.org/10.1042/BST20130269</a>","ista":"Chen X, Friml J. 2014. Rho-GTPase-regulated vesicle trafficking in plant cell polarity. Biochemical Society Transactions. 42(1), 212–218.","chicago":"Chen, Xu, and Jiří Friml. “Rho-GTPase-Regulated Vesicle Trafficking in Plant Cell Polarity.” <i>Biochemical Society Transactions</i>. Portland Press, 2014. <a href=\"https://doi.org/10.1042/BST20130269\">https://doi.org/10.1042/BST20130269</a>."}},{"issue":"6174","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publisher":"American Association for the Advancement of Science","acknowledgement":"Supported by the intramural research program of the National Institute of Arthritis and Musculoskeletal and Skin Diseases and by its Laboratory Animal Care and Use Section and Flow Cytometry Group, Office of Science and Technology","citation":{"ista":"Xu T, Dai N, Chen J, Nagawa S, Cao M, Li H, Zhou Z, Chen X, De Rycke R, Rakusová H, Wang W, Jones A, Friml J, Patterson S, Bleecker A, Yang Z. 2014. Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. Science. 343(6174), 1025–1028.","chicago":"Xu, Tongda, Ning Dai, Jisheng Chen, Shingo Nagawa, Min Cao, Hongjiang Li, Zimin Zhou, et al. “Cell Surface ABP1-TMK Auxin Sensing Complex Activates ROP GTPase Signaling.” <i>Science</i>. American Association for the Advancement of Science, 2014. <a href=\"https://doi.org/10.1126/science.1245125\">https://doi.org/10.1126/science.1245125</a>.","ama":"Xu T, Dai N, Chen J, et al. Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. <i>Science</i>. 2014;343(6174):1025-1028. doi:<a href=\"https://doi.org/10.1126/science.1245125\">10.1126/science.1245125</a>","ieee":"T. Xu <i>et al.</i>, “Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling,” <i>Science</i>, vol. 343, no. 6174. American Association for the Advancement of Science, pp. 1025–1028, 2014.","mla":"Xu, Tongda, et al. “Cell Surface ABP1-TMK Auxin Sensing Complex Activates ROP GTPase Signaling.” <i>Science</i>, vol. 343, no. 6174, American Association for the Advancement of Science, 2014, pp. 1025–28, doi:<a href=\"https://doi.org/10.1126/science.1245125\">10.1126/science.1245125</a>.","short":"T. Xu, N. Dai, J. Chen, S. Nagawa, M. Cao, H. Li, Z. Zhou, X. Chen, R. De Rycke, H. Rakusová, W. Wang, A. Jones, J. Friml, S. Patterson, A. Bleecker, Z. Yang, Science 343 (2014) 1025–1028.","apa":"Xu, T., Dai, N., Chen, J., Nagawa, S., Cao, M., Li, H., … Yang, Z. (2014). Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1245125\">https://doi.org/10.1126/science.1245125</a>"},"_id":"1917","department":[{"_id":"JiFr"}],"scopus_import":"1","status":"public","day":"28","author":[{"full_name":"Xu, Tongda","last_name":"Xu","first_name":"Tongda"},{"first_name":"Ning","full_name":"Dai, Ning","last_name":"Dai"},{"last_name":"Chen","full_name":"Chen, Jisheng","first_name":"Jisheng"},{"first_name":"Shingo","full_name":"Nagawa, Shingo","last_name":"Nagawa"},{"first_name":"Min","full_name":"Cao, Min","last_name":"Cao"},{"last_name":"Li","full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","first_name":"Hongjiang","orcid":"0000-0001-5039-9660"},{"full_name":"Zhou, Zimin","last_name":"Zhou","first_name":"Zimin"},{"last_name":"Chen","full_name":"Chen, Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","first_name":"Xu"},{"first_name":"Riet","full_name":"De Rycke, Riet","last_name":"De Rycke"},{"last_name":"Rakusová","full_name":"Rakusová, Hana","first_name":"Hana"},{"first_name":"Wen","last_name":"Wang","full_name":"Wang, Wen"},{"first_name":"Alan","last_name":"Jones","full_name":"Jones, Alan"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jirí"},{"first_name":"Sara","last_name":"Patterson","full_name":"Patterson, Sara"},{"first_name":"Anthony","full_name":"Bleecker, Anthony","last_name":"Bleecker"},{"first_name":"Zhenbiao","full_name":"Yang, Zhenbiao","last_name":"Yang"}],"language":[{"iso":"eng"}],"external_id":{"isi":["000332309600046"],"pmid":["24578577"]},"abstract":[{"lang":"eng","text":"Auxin-binding protein 1 (ABP1) was discovered nearly 40 years ago and was shown to be essential for plant development and morphogenesis, but its mode of action remains unclear. Here, we report that the plasma membrane-localized transmembrane kinase (TMK) receptor-like kinases interact with ABP1 and transduce auxin signal to activate plasma membrane-associated ROPs [Rho-like guanosine triphosphatases (GTPase) from plants], leading to changes in the cytoskeleton and the shape of leaf pavement cells in Arabidopsis. The interaction between ABP1 and TMK at the cell surface is induced by auxin and requires ABP1 sensing of auxin. These findings show that TMK proteins and ABP1 form a cell surface auxin perception complex that activates ROP signaling pathways, regulating nontranscriptional cytoplasmic responses and associated fundamental processes."}],"intvolume":"       343","isi":1,"volume":343,"year":"2014","type":"journal_article","date_updated":"2025-09-29T12:20:10Z","date_created":"2018-12-11T11:54:42Z","fulldoi":"https://doi.org/10.1126/science.1245125","publist_id":"5177","publication_status":"published","publication":"Science","doi":"10.1126/science.1245125","date_published":"2014-02-28T00:00:00Z","page":"1025 - 1028","quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166562/"}],"pmid":1,"oa_version":"Submitted Version","article_type":"original","oa":1,"month":"02","article_processing_charge":"No","title":"Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling"},{"type":"journal_article","year":"2014","publication_status":"published","publication":"Plant Cell","publist_id":"5173","fulldoi":"https://doi.org/10.1105/tpc.114.126185","date_created":"2018-12-11T11:54:43Z","date_updated":"2025-09-29T12:17:33Z","intvolume":"        26","volume":26,"isi":1,"month":"05","oa":1,"title":"Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis","article_processing_charge":"No","date_published":"2014-05-01T00:00:00Z","doi":"10.1105/tpc.114.126185","oa_version":"Submitted Version","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079372/","open_access":"1"}],"corr_author":"1","page":"2114 - 2128","publisher":"American Society of Plant Biologists","_id":"1921","scopus_import":"1","department":[{"_id":"JiFr"}],"citation":{"ista":"Tejos R, Sauer M, Vanneste S, Palacios-Gomez M, Li H, Heilmann M, Van Wijk R, Vermeer J, Heilmann I, Munnik T, Friml J. 2014. Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis. Plant Cell. 26(5), 2114–2128.","chicago":"Tejos, Ricardo, Michael Sauer, Steffen Vanneste, MiriamPalacios  Palacios-Gomez, Hongjiang Li, Mareike Heilmann, Ringo Van Wijk, et al. “Bipolar Plasma Membrane Distribution of Phosphoinositides and Their Requirement for Auxin-Mediated Cell Polarity and Patterning in Arabidopsis.” <i>Plant Cell</i>. American Society of Plant Biologists, 2014. <a href=\"https://doi.org/10.1105/tpc.114.126185\">https://doi.org/10.1105/tpc.114.126185</a>.","ama":"Tejos R, Sauer M, Vanneste S, et al. Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis. <i>Plant Cell</i>. 2014;26(5):2114-2128. doi:<a href=\"https://doi.org/10.1105/tpc.114.126185\">10.1105/tpc.114.126185</a>","ieee":"R. Tejos <i>et al.</i>, “Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis,” <i>Plant Cell</i>, vol. 26, no. 5. American Society of Plant Biologists, pp. 2114–2128, 2014.","mla":"Tejos, Ricardo, et al. “Bipolar Plasma Membrane Distribution of Phosphoinositides and Their Requirement for Auxin-Mediated Cell Polarity and Patterning in Arabidopsis.” <i>Plant Cell</i>, vol. 26, no. 5, American Society of Plant Biologists, 2014, pp. 2114–28, doi:<a href=\"https://doi.org/10.1105/tpc.114.126185\">10.1105/tpc.114.126185</a>.","short":"R. Tejos, M. Sauer, S. Vanneste, M. Palacios-Gomez, H. Li, M. Heilmann, R. Van Wijk, J. Vermeer, I. Heilmann, T. Munnik, J. Friml, Plant Cell 26 (2014) 2114–2128.","apa":"Tejos, R., Sauer, M., Vanneste, S., Palacios-Gomez, M., Li, H., Heilmann, M., … Friml, J. (2014). Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.114.126185\">https://doi.org/10.1105/tpc.114.126185</a>"},"acknowledgement":"This work was supported by grants from the Odysseus program of the Research Foundation-Flanders (to J.F.).","ec_funded":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"5","external_id":{"isi":["000338771700027"]},"abstract":[{"text":"Cell polarity manifested by asymmetric distribution of cargoes, such as receptors and transporters, within the plasma membrane (PM) is crucial for essential functions in multicellular organisms. In plants, cell polarity (re)establishment is intimately linked to patterning processes. Despite the importance of cell polarity, its underlying mechanisms are still largely unknown, including the definition and distinctiveness of the polar domains within the PM. Here, we show in Arabidopsis thaliana that the signaling membrane components, the phosphoinositides phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4, 5-bisphosphate [PtdIns(4, 5)P2] as well as PtdIns4P 5-kinases mediating their interconversion, are specifically enriched at apical and basal polar plasma membrane domains. The PtdIns4P 5-kinases PIP5K1 and PIP5K2 are redundantly required for polar localization of specifically apical and basal cargoes, such as PIN-FORMED transporters for the plant hormone auxin. As a consequence of the polarity defects, instructive auxin gradients as well as embryonic and postembryonic patterning are severely compromised. Furthermore, auxin itself regulates PIP5K transcription and PtdIns4P and PtdIns(4, 5)P2 levels, in particular their association with polar PM domains. Our results provide insight into the polar domain-delineating mechanisms in plant cells that depend on apical and basal distribution of membrane lipids and are essential for embryonic and postembryonic patterning.","lang":"eng"}],"project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300"}],"status":"public","language":[{"iso":"eng"}],"author":[{"first_name":"Ricardo","full_name":"Tejos, Ricardo","last_name":"Tejos"},{"first_name":"Michael","full_name":"Sauer, Michael","last_name":"Sauer"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"},{"first_name":"MiriamPalacios ","full_name":"Palacios-Gomez, MiriamPalacios ","last_name":"Palacios-Gomez"},{"first_name":"Hongjiang","orcid":"0000-0001-5039-9660","full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","last_name":"Li"},{"full_name":"Heilmann, Mareike","last_name":"Heilmann","first_name":"Mareike"},{"first_name":"Ringo","full_name":"Van Wijk, Ringo","last_name":"Van Wijk"},{"full_name":"Vermeer, Joop","last_name":"Vermeer","first_name":"Joop"},{"last_name":"Heilmann","full_name":"Heilmann, Ingo","first_name":"Ingo"},{"last_name":"Munnik","full_name":"Munnik, Teun","first_name":"Teun"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"day":"01"},{"day":"27","author":[{"full_name":"Le, Jie","last_name":"Le","first_name":"Jie"},{"full_name":"Liu, Xuguang","last_name":"Liu","first_name":"Xuguang"},{"last_name":"Yang","full_name":"Yang, Kezhen","first_name":"Kezhen"},{"last_name":"Chen","full_name":"Chen, Xiaolan","first_name":"Xiaolan"},{"first_name":"Lingling","last_name":"Zhu","full_name":"Zhu, Lingling"},{"full_name":"Wang, Hongzhe","last_name":"Wang","first_name":"Hongzhe"},{"last_name":"Wang","full_name":"Wang, Ming","first_name":"Ming"},{"first_name":"Steffen","full_name":"Vanneste, Steffen","last_name":"Vanneste"},{"first_name":"Miyo","full_name":"Morita, Miyo","last_name":"Morita"},{"full_name":"Tasaka, Masao","last_name":"Tasaka","first_name":"Masao"},{"first_name":"Zhaojun","last_name":"Ding","full_name":"Ding, Zhaojun"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Beeckman","full_name":"Beeckman, Tom","first_name":"Tom"},{"full_name":"Sack, Fred","last_name":"Sack","first_name":"Fred"}],"language":[{"iso":"eng"}],"status":"public","external_id":{"isi":["000331084200032"]},"abstract":[{"lang":"eng","text":"Stomata are two-celled valves that control epidermal pores whose spacing optimizes shoot-atmosphere gas exchange. They develop from protodermal cells after unequal divisions followed by an equal division and differentiation. The concentration of the hormone auxin, a master plant developmental regulator, is tightly controlled in time and space, but its role, if any, in stomatal formation is obscure. Here dynamic changes of auxin activity during stomatal development are monitored using auxin input (DII-VENUS) and output (DR5:VENUS) markers by time-lapse imaging. A decrease in auxin levels in the smaller daughter cell after unequal division presages the acquisition of a guard mother cell fate whose equal division produces the two guard cells. Thus, stomatal patterning requires auxin pathway control of stem cell compartment size, as well as auxin depletion that triggers a developmental switch from unequal to equal division."}],"article_number":"3090","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"chicago":"Le, Jie, Xuguang Liu, Kezhen Yang, Xiaolan Chen, Lingling Zhu, Hongzhe Wang, Ming Wang, et al. “Auxin Transport and Activity Regulate Stomatal Patterning and Development.” <i>Nature Communications</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/ncomms4090\">https://doi.org/10.1038/ncomms4090</a>.","ista":"Le J, Liu X, Yang K, Chen X, Zhu L, Wang H, Wang M, Vanneste S, Morita M, Tasaka M, Ding Z, Friml J, Beeckman T, Sack F. 2014. Auxin transport and activity regulate stomatal patterning and development. Nature Communications. 5, 3090.","short":"J. Le, X. Liu, K. Yang, X. Chen, L. Zhu, H. Wang, M. Wang, S. Vanneste, M. Morita, M. Tasaka, Z. Ding, J. Friml, T. Beeckman, F. Sack, Nature Communications 5 (2014).","apa":"Le, J., Liu, X., Yang, K., Chen, X., Zhu, L., Wang, H., … Sack, F. (2014). Auxin transport and activity regulate stomatal patterning and development. <i>Nature Communications</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncomms4090\">https://doi.org/10.1038/ncomms4090</a>","mla":"Le, Jie, et al. “Auxin Transport and Activity Regulate Stomatal Patterning and Development.” <i>Nature Communications</i>, vol. 5, 3090, Nature Publishing Group, 2014, doi:<a href=\"https://doi.org/10.1038/ncomms4090\">10.1038/ncomms4090</a>.","ieee":"J. Le <i>et al.</i>, “Auxin transport and activity regulate stomatal patterning and development,” <i>Nature Communications</i>, vol. 5. Nature Publishing Group, 2014.","ama":"Le J, Liu X, Yang K, et al. Auxin transport and activity regulate stomatal patterning and development. <i>Nature Communications</i>. 2014;5. doi:<a href=\"https://doi.org/10.1038/ncomms4090\">10.1038/ncomms4090</a>"},"_id":"1924","department":[{"_id":"JiFr"}],"scopus_import":"1","publisher":"Nature Publishing Group","quality_controlled":"1","oa_version":"None","doi":"10.1038/ncomms4090","date_published":"2014-01-27T00:00:00Z","article_processing_charge":"No","title":"Auxin transport and activity regulate stomatal patterning and development","month":"01","isi":1,"volume":5,"intvolume":"         5","date_updated":"2025-09-29T12:15:23Z","fulldoi":"https://doi.org/10.1038/ncomms4090","date_created":"2018-12-11T11:54:44Z","publist_id":"5170","publication":"Nature Communications","publication_status":"published","year":"2014","type":"journal_article"},{"publisher":"Cell Press","citation":{"chicago":"Marhavý, Peter, Jérôme Duclercq, Benjamin Weller, Elena Feraru, Agnieszka Bielach, Remko Offringa, Jiří Friml, Claus Schwechheimer, Angus Murphy, and Eva Benková. “Cytokinin Controls Polarity of PIN1-Dependent Auxin Transport during Lateral Root Organogenesis.” <i>Current Biology</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cub.2014.04.002\">https://doi.org/10.1016/j.cub.2014.04.002</a>.","ista":"Marhavý P, Duclercq J, Weller B, Feraru E, Bielach A, Offringa R, Friml J, Schwechheimer C, Murphy A, Benková E. 2014. Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis. Current Biology. 24(9), 1031–1037.","apa":"Marhavý, P., Duclercq, J., Weller, B., Feraru, E., Bielach, A., Offringa, R., … Benková, E. (2014). Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2014.04.002\">https://doi.org/10.1016/j.cub.2014.04.002</a>","short":"P. Marhavý, J. Duclercq, B. Weller, E. Feraru, A. Bielach, R. Offringa, J. Friml, C. Schwechheimer, A. Murphy, E. Benková, Current Biology 24 (2014) 1031–1037.","ama":"Marhavý P, Duclercq J, Weller B, et al. Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis. <i>Current Biology</i>. 2014;24(9):1031-1037. doi:<a href=\"https://doi.org/10.1016/j.cub.2014.04.002\">10.1016/j.cub.2014.04.002</a>","mla":"Marhavý, Peter, et al. “Cytokinin Controls Polarity of PIN1-Dependent Auxin Transport during Lateral Root Organogenesis.” <i>Current Biology</i>, vol. 24, no. 9, Cell Press, 2014, pp. 1031–37, doi:<a href=\"https://doi.org/10.1016/j.cub.2014.04.002\">10.1016/j.cub.2014.04.002</a>.","ieee":"P. Marhavý <i>et al.</i>, “Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis,” <i>Current Biology</i>, vol. 24, no. 9. Cell Press, pp. 1031–1037, 2014."},"_id":"1934","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"scopus_import":"1","ec_funded":1,"issue":"9","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"text":"The plant hormones auxin and cytokinin mutually coordinate their activities to control various aspects of development [1-9], and their crosstalk occurs at multiple levels [10, 11]. Cytokinin-mediated modulation of auxin transport provides an efficient means to regulate auxin distribution in plant organs. Here, we demonstrate that cytokinin does not merely control the overall auxin flow capacity, but might also act as a polarizing cue and control the auxin stream directionality during plant organogenesis. Cytokinin enhances the PIN-FORMED1 (PIN1) auxin transporter depletion at specific polar domains, thus rearranging the cellular PIN polarities and directly regulating the auxin flow direction. This selective cytokinin sensitivity correlates with the PIN protein phosphorylation degree. PIN1 phosphomimicking mutations, as well as enhanced phosphorylation in plants with modulated activities of PIN-specific kinases and phosphatases, desensitize PIN1 to cytokinin. Our results reveal conceptually novel, cytokinin-driven polarization mechanism that operates in developmental processes involving rapid auxin stream redirection, such as lateral root organogenesis, in which a gradual PIN polarity switch defines the growth axis of the newly formed organ.","lang":"eng"}],"external_id":{"isi":["000335542300029"]},"project":[{"call_identifier":"FP7","name":"Hormonal cross-talk in plant organogenesis","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","grant_number":"207362"}],"status":"public","day":"05","author":[{"last_name":"Marhavy","full_name":"Marhavy, Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","first_name":"Peter","orcid":"0000-0001-5227-5741"},{"full_name":"Duclercq, Jérôme","last_name":"Duclercq","first_name":"Jérôme"},{"last_name":"Weller","full_name":"Weller, Benjamin","first_name":"Benjamin"},{"last_name":"Feraru","full_name":"Feraru, Elena","first_name":"Elena"},{"first_name":"Agnieszka","last_name":"Bielach","full_name":"Bielach, Agnieszka"},{"first_name":"Remko","full_name":"Offringa, Remko","last_name":"Offringa"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"first_name":"Claus","last_name":"Schwechheimer","full_name":"Schwechheimer, Claus"},{"first_name":"Angus","full_name":"Murphy, Angus","last_name":"Murphy"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková"}],"language":[{"iso":"eng"}],"year":"2014","type":"journal_article","date_updated":"2025-09-29T12:09:47Z","date_created":"2018-12-11T11:54:48Z","fulldoi":"https://doi.org/10.1016/j.cub.2014.04.002","publication_status":"published","publist_id":"5160","publication":"Current Biology","intvolume":"        24","volume":24,"isi":1,"month":"05","article_processing_charge":"No","title":"Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis","doi":"10.1016/j.cub.2014.04.002","date_published":"2014-05-05T00:00:00Z","page":"1031 - 1037","corr_author":"1","quality_controlled":"1","oa_version":"None"},{"ec_funded":1,"issue":"23","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publisher":"Cell Press","citation":{"chicago":"Viaene, Tom, Katarina Landberg, Mattias Thelander, Eva Medvecka, Eric Pederson, Elena Feraru, Endymion Cooper, et al. “Directional Auxin Transport Mechanisms in Early Diverging Land Plants.” <i>Current Biology</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cub.2014.09.056\">https://doi.org/10.1016/j.cub.2014.09.056</a>.","ista":"Viaene T, Landberg K, Thelander M, Medvecka E, Pederson E, Feraru E, Cooper E, Karimi M, Delwiche C, Ljung K, Geisler M, Sundberg E, Friml J. 2014. Directional auxin transport mechanisms in early diverging land plants. Current Biology. 24(23), 2786–2791.","short":"T. Viaene, K. Landberg, M. Thelander, E. Medvecka, E. Pederson, E. Feraru, E. Cooper, M. Karimi, C. Delwiche, K. Ljung, M. Geisler, E. Sundberg, J. Friml, Current Biology 24 (2014) 2786–2791.","apa":"Viaene, T., Landberg, K., Thelander, M., Medvecka, E., Pederson, E., Feraru, E., … Friml, J. (2014). Directional auxin transport mechanisms in early diverging land plants. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2014.09.056\">https://doi.org/10.1016/j.cub.2014.09.056</a>","ieee":"T. Viaene <i>et al.</i>, “Directional auxin transport mechanisms in early diverging land plants,” <i>Current Biology</i>, vol. 24, no. 23. Cell Press, pp. 2786–2791, 2014.","mla":"Viaene, Tom, et al. “Directional Auxin Transport Mechanisms in Early Diverging Land Plants.” <i>Current Biology</i>, vol. 24, no. 23, Cell Press, 2014, pp. 2786–91, doi:<a href=\"https://doi.org/10.1016/j.cub.2014.09.056\">10.1016/j.cub.2014.09.056</a>.","ama":"Viaene T, Landberg K, Thelander M, et al. Directional auxin transport mechanisms in early diverging land plants. <i>Current Biology</i>. 2014;24(23):2786-2791. doi:<a href=\"https://doi.org/10.1016/j.cub.2014.09.056\">10.1016/j.cub.2014.09.056</a>"},"_id":"1994","scopus_import":"1","department":[{"_id":"JiFr"}],"project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300"}],"status":"public","day":"01","author":[{"first_name":"Tom","full_name":"Viaene, Tom","last_name":"Viaene"},{"last_name":"Landberg","full_name":"Landberg, Katarina","first_name":"Katarina"},{"first_name":"Mattias","full_name":"Thelander, Mattias","last_name":"Thelander"},{"full_name":"Medvecka, Eva","last_name":"Medvecka","first_name":"Eva"},{"first_name":"Eric","last_name":"Pederson","full_name":"Pederson, Eric"},{"first_name":"Elena","last_name":"Feraru","full_name":"Feraru, Elena"},{"first_name":"Endymion","last_name":"Cooper","full_name":"Cooper, Endymion"},{"full_name":"Karimi, Mansour","last_name":"Karimi","first_name":"Mansour"},{"last_name":"Delwiche","full_name":"Delwiche, Charles","first_name":"Charles"},{"last_name":"Ljung","full_name":"Ljung, Karin","first_name":"Karin"},{"first_name":"Markus","full_name":"Geisler, Markus","last_name":"Geisler"},{"first_name":"Eva","full_name":"Sundberg, Eva","last_name":"Sundberg"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí"}],"language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"The emergence and radiation of multicellular land plants was driven by crucial innovations to their body plans [1]. The directional transport of the phytohormone auxin represents a key, plant-specific mechanism for polarization and patterning in complex seed plants [2-5]. Here, we show that already in the early diverging land plant lineage, as exemplified by the moss Physcomitrella patens, auxin transport by PIN transporters is operational and diversified into ER-localized and plasma membrane-localized PIN proteins. Gain-of-function and loss-of-function analyses revealed that PIN-dependent intercellular auxin transport in Physcomitrella mediates crucial developmental transitions in tip-growing filaments and waves of polarization and differentiation in leaf-like structures. Plasma membrane PIN proteins localize in a polar manner to the tips of moss filaments, revealing an unexpected relation between polarization mechanisms in moss tip-growing cells and multicellular tissues of seed plants. Our results trace the origins of polarization and auxin-mediated patterning mechanisms and highlight the crucial role of polarized auxin transport during the evolution of multicellular land plants."}],"external_id":{"isi":["000345808700019"]},"intvolume":"        24","volume":24,"isi":1,"year":"2014","type":"journal_article","date_updated":"2025-09-29T12:07:20Z","fulldoi":"https://doi.org/10.1016/j.cub.2014.09.056","date_created":"2018-12-11T11:55:06Z","publication":"Current Biology","publication_status":"published","publist_id":"5088","doi":"10.1016/j.cub.2014.09.056","date_published":"2014-12-01T00:00:00Z","page":"2786 - 2791","corr_author":"1","quality_controlled":"1","oa_version":"None","month":"12","article_processing_charge":"No","title":"Directional auxin transport mechanisms in early diverging land plants"}]