[{"doi":"10.1111/tpj.12373","publisher":"Wiley-Blackwell","_id":"2249","title":"Inter-regulation of the unfolded protein response and auxin signaling","day":"01","year":"2014","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","month":"01","fulldoi":"https://doi.org/10.1111/tpj.12373","quality_controlled":"1","publication_identifier":{"issn":["0960-7412"]},"date_updated":"2026-04-16T10:08:30Z","volume":77,"oa_version":"Submitted Version","isi":1,"scopus_import":"1","publist_id":"4699","publication":"Plant Journal","status":"public","external_id":{"isi":["000328661300008"]},"language":[{"iso":"eng"}],"oa":1,"article_processing_charge":"No","intvolume":"        77","abstract":[{"text":"The unfolded protein response (UPR) is a signaling network triggered by overload of protein-folding demand in the endoplasmic reticulum (ER), a condition termed ER stress. The UPR is critical for growth and development; nonetheless, connections between the UPR and other cellular regulatory processes remain largely unknown. Here, we identify a link between the UPR and the phytohormone auxin, a master regulator of plant physiology. We show that ER stress triggers down-regulation of auxin receptors and transporters in Arabidopsis thaliana. We also demonstrate that an Arabidopsis mutant of a conserved ER stress sensor IRE1 exhibits defects in the auxin response and levels. These data not only support that the plant IRE1 is required for auxin homeostasis, they also reveal a species-specific feature of IRE1 in multicellular eukaryotes. Furthermore, by establishing that UPR activation is reduced in mutants of ER-localized auxin transporters, including PIN5, we define a long-neglected biological significance of ER-based auxin regulation. We further examine the functional relationship of IRE1 and PIN5 by showing that an ire1 pin5 triple mutant enhances defects of UPR activation and auxin homeostasis in ire1 or pin5. Our results imply that the plant UPR has evolved a hormone-dependent strategy for coordinating ER function with physiological processes.","lang":"eng"}],"date_published":"2014-01-01T00:00:00Z","department":[{"_id":"JiFr"}],"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981873/","open_access":"1"}],"type":"journal_article","issue":"1","author":[{"last_name":"Chen","full_name":"Chen, Yani","first_name":"Yani"},{"full_name":"Aung, Kyaw","first_name":"Kyaw","last_name":"Aung"},{"first_name":"Jakub","full_name":"Rolčík, Jakub","last_name":"Rolčík"},{"first_name":"Kathryn","full_name":"Walicki, Kathryn","last_name":"Walicki"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Federica","full_name":"Brandizzí, Federica","last_name":"Brandizzí"}],"page":"97 - 107","date_created":"2018-12-11T11:56:34Z","publication_status":"published","citation":{"short":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, F. Brandizzí, Plant Journal 77 (2014) 97–107.","apa":"Chen, Y., Aung, K., Rolčík, J., Walicki, K., Friml, J., &#38; Brandizzí, F. (2014). Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>","ista":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. 2014. Inter-regulation of the unfolded protein response and auxin signaling. Plant Journal. 77(1), 97–107.","mla":"Chen, Yani, et al. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 97–107, doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>.","chicago":"Chen, Yani, Kyaw Aung, Jakub Rolčík, Kathryn Walicki, Jiří Friml, and Federica Brandizzí. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>.","ama":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. 2014;77(1):97-107. doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>","ieee":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, and F. Brandizzí, “Inter-regulation of the unfolded protein response and auxin signaling,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 97–107, 2014."}},{"oa_version":"Published Version","isi":1,"fulldoi":"https://doi.org/10.1111/tpj.12369","publication_identifier":{"issn":["0960-7412"]},"quality_controlled":"1","date_updated":"2026-06-18T18:33:25Z","volume":77,"article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"01","doi":"10.1111/tpj.12369","publisher":"Wiley-Blackwell","_id":"2253","title":"Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth","day":"01","year":"2014","publication_status":"published","project":[{"_id":"256BDAB0-B435-11E9-9278-68D0E5697425","name":"Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur"}],"citation":{"short":"A. Bailly, B. Wang, M. Zwiewka, S. Pollmann, D. Schenck, H. Lüthen, A. Schulz, J. Friml, M. Geisler, Plant Journal 77 (2014) 108–118.","apa":"Bailly, A., Wang, B., Zwiewka, M., Pollmann, S., Schenck, D., Lüthen, H., … Geisler, M. (2014). Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>","ista":"Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M. 2014. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. Plant Journal. 77(1), 108–118.","ieee":"A. Bailly <i>et al.</i>, “Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 108–118, 2014.","chicago":"Bailly, Aurélien, Bangjun Wang, Marta Zwiewka, Stephan Pollmann, Daniel Schenck, Hartwig Lüthen, Alexander Schulz, Jiří Friml, and Markus Geisler. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>.","mla":"Bailly, Aurélien, et al. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 108–18, doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>.","ama":"Bailly A, Wang B, Zwiewka M, et al. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. 2014;77(1):108-118. doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>"},"type":"journal_article","issue":"1","author":[{"full_name":"Bailly, Aurélien","first_name":"Aurélien","last_name":"Bailly"},{"last_name":"Wang","full_name":"Wang, Bangjun","first_name":"Bangjun"},{"last_name":"Zwiewka","first_name":"Marta","full_name":"Zwiewka, Marta"},{"last_name":"Pollmann","full_name":"Pollmann, Stephan","first_name":"Stephan"},{"last_name":"Schenck","first_name":"Daniel","full_name":"Schenck, Daniel"},{"first_name":"Hartwig","full_name":"Lüthen, Hartwig","last_name":"Lüthen"},{"last_name":"Schulz","first_name":"Alexander","full_name":"Schulz, Alexander"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"last_name":"Geisler","first_name":"Markus","full_name":"Geisler, Markus"}],"page":"108 - 118","date_created":"2018-12-11T11:56:35Z","language":[{"iso":"eng"}],"oa":1,"external_id":{"isi":["000328661300009"]},"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor- glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct. As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation."}],"intvolume":"        77","department":[{"_id":"JiFr"}],"date_published":"2014-01-01T00:00:00Z","main_file_link":[{"url":"https://doi.org/10.1111/tpj.12369","open_access":"1"}],"ddc":["580"],"scopus_import":"1","status":"public","publication":"Plant Journal","publist_id":"4694"},{"article_number":"140017","date_created":"2018-12-11T11:56:13Z","type":"journal_article","author":[{"last_name":"Kania","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","full_name":"Kania, Urszula","first_name":"Urszula"},{"first_name":"Matyas","full_name":"Fendrych, Matyas","last_name":"Fendrych"},{"full_name":"Friml, Jiřĺ","orcid":"0000-0002-8302-7596","first_name":"Jiřĺ","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"issue":"APRIL","file_date_updated":"2020-07-14T12:45:31Z","citation":{"ista":"Kania U, Fendrych M, Friml J. 2014. Polar delivery in plants; commonalities and differences to animal epithelial cells. Open Biology. 4(APRIL), 140017.","chicago":"Kania, Urszula, Matyas Fendrych, and Jiří Friml. “Polar Delivery in Plants; Commonalities and Differences to Animal Epithelial Cells.” <i>Open Biology</i>. Royal Society, 2014. <a href=\"https://doi.org/10.1098/rsob.140017\">https://doi.org/10.1098/rsob.140017</a>.","ama":"Kania U, Fendrych M, Friml J. Polar delivery in plants; commonalities and differences to animal epithelial cells. <i>Open Biology</i>. 2014;4(APRIL). doi:<a href=\"https://doi.org/10.1098/rsob.140017\">10.1098/rsob.140017</a>","ieee":"U. Kania, M. Fendrych, and J. Friml, “Polar delivery in plants; commonalities and differences to animal epithelial cells,” <i>Open Biology</i>, vol. 4, no. APRIL. Royal Society, 2014.","mla":"Kania, Urszula, et al. “Polar Delivery in Plants; Commonalities and Differences to Animal Epithelial Cells.” <i>Open Biology</i>, vol. 4, no. APRIL, 140017, Royal Society, 2014, doi:<a href=\"https://doi.org/10.1098/rsob.140017\">10.1098/rsob.140017</a>.","short":"U. Kania, M. Fendrych, J. Friml, Open Biology 4 (2014).","apa":"Kania, U., Fendrych, M., &#38; Friml, J. (2014). Polar delivery in plants; commonalities and differences to animal epithelial cells. <i>Open Biology</i>. Royal Society. <a href=\"https://doi.org/10.1098/rsob.140017\">https://doi.org/10.1098/rsob.140017</a>"},"pubrep_id":"441","file":[{"access_level":"open_access","file_size":682570,"creator":"system","file_name":"IST-2016-441-v1+1_140017.full.pdf","content_type":"application/pdf","date_updated":"2020-07-14T12:45:31Z","date_created":"2018-12-12T10:13:40Z","relation":"main_file","file_id":"5025","checksum":"2020627feff36cf0799167c84149fa75"}],"publication_status":"published","license":"https://creativecommons.org/licenses/by/4.0/","publication":"Open Biology","status":"public","publist_id":"4786","ddc":["570"],"scopus_import":"1","has_accepted_license":"1","intvolume":"         4","abstract":[{"lang":"eng","text":"Although plant and animal cells use a similar core mechanism to deliver proteins to the plasma membrane, their different lifestyle, body organization and specific cell structures resulted in the acquisition of regulatory mechanisms that vary in the two kingdoms. In particular, cell polarity regulators do not seem to be conserved, because genes encoding key components are absent in plant genomes. In plants, the broad knowledge on polarity derives from the study of auxin transporters, the PIN-FORMED proteins, in the model plant Arabidopsis thaliana. In animals, much information is provided from the study of polarity in epithelial cells that exhibit basolateral and luminal apical polarities, separated by tight junctions. In this review, we summarize the similarities and differences of the polarization mechanisms between plants and animals and survey the main genetic approaches that have been used to characterize new genes involved in polarity establishment in plants, including the frequently used forward and reverse genetics screens as well as a novel chemical genetics approach that is expected to overcome the limitation of classical genetics methods."}],"date_published":"2014-04-16T00:00:00Z","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"external_id":{"isi":["000335813800006"]},"oa":1,"article_processing_charge":"No","volume":4,"date_updated":"2025-09-29T11:32:17Z","quality_controlled":"1","fulldoi":"https://doi.org/10.1098/rsob.140017","isi":1,"oa_version":"Published Version","day":"16","year":"2014","corr_author":"1","title":"Polar delivery in plants; commonalities and differences to animal epithelial cells","doi":"10.1098/rsob.140017","acknowledgement":"This work was supported by a grant from the Research Foundation-Flanders (Odysseus).\r\n\r\n","_id":"2188","publisher":"Royal Society","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"month":"04","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"quality_controlled":"1","fulldoi":"https://doi.org/10.1016/j.cub.2014.08.036","volume":24,"date_updated":"2025-09-29T13:10:45Z","oa_version":"Submitted Version","isi":1,"doi":"10.1016/j.cub.2014.08.036","acknowledgement":"This work was funded by grants from EraSysBio+ (iSAM) and ERC (Morphodynamics). ","_id":"1852","publisher":"Cell Press","day":"06","year":"2014","title":"An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis","month":"10","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"2335 - 2342","date_created":"2018-12-11T11:54:22Z","type":"journal_article","author":[{"last_name":"Sassi","first_name":"Massimiliano","full_name":"Sassi, Massimiliano"},{"full_name":"Ali, Olivier","first_name":"Olivier","last_name":"Ali"},{"full_name":"Boudon, Frédéric","first_name":"Frédéric","last_name":"Boudon"},{"full_name":"Cloarec, Gladys","first_name":"Gladys","last_name":"Cloarec"},{"last_name":"Abad","first_name":"Ursula","full_name":"Abad, Ursula"},{"last_name":"Cellier","first_name":"Coralie","full_name":"Cellier, Coralie"},{"full_name":"Chen, Xu","first_name":"Xu","last_name":"Chen","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Gilles","first_name":"Benjamin","full_name":"Gilles, Benjamin"},{"last_name":"Milani","first_name":"Pascale","full_name":"Milani, Pascale"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"},{"last_name":"Vernoux","full_name":"Vernoux, Teva","first_name":"Teva"},{"full_name":"Godin, Christophe","first_name":"Christophe","last_name":"Godin"},{"first_name":"Olivier","full_name":"Hamant, Olivier","last_name":"Hamant"},{"last_name":"Traas","first_name":"Jan","full_name":"Traas, Jan"}],"issue":"19","publication_status":"published","citation":{"ieee":"M. Sassi <i>et al.</i>, “An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis,” <i>Current Biology</i>, vol. 24, no. 19. Cell Press, pp. 2335–2342, 2014.","chicago":"Sassi, Massimiliano, Olivier Ali, Frédéric Boudon, Gladys Cloarec, Ursula Abad, Coralie Cellier, Xu Chen, et al. “An Auxin-Mediated Shift toward Growth Isotropy Promotes Organ Formation at the Shoot Meristem in Arabidopsis.” <i>Current Biology</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">https://doi.org/10.1016/j.cub.2014.08.036</a>.","ama":"Sassi M, Ali O, Boudon F, et al. An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. <i>Current Biology</i>. 2014;24(19):2335-2342. doi:<a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">10.1016/j.cub.2014.08.036</a>","mla":"Sassi, Massimiliano, et al. “An Auxin-Mediated Shift toward Growth Isotropy Promotes Organ Formation at the Shoot Meristem in Arabidopsis.” <i>Current Biology</i>, vol. 24, no. 19, Cell Press, 2014, pp. 2335–42, doi:<a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">10.1016/j.cub.2014.08.036</a>.","ista":"Sassi M, Ali O, Boudon F, Cloarec G, Abad U, Cellier C, Chen X, Gilles B, Milani P, Friml J, Vernoux T, Godin C, Hamant O, Traas J. 2014. An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. Current Biology. 24(19), 2335–2342.","apa":"Sassi, M., Ali, O., Boudon, F., Cloarec, G., Abad, U., Cellier, C., … Traas, J. (2014). An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2014.08.036\">https://doi.org/10.1016/j.cub.2014.08.036</a>","short":"M. Sassi, O. Ali, F. Boudon, G. Cloarec, U. Abad, C. Cellier, X. Chen, B. Gilles, P. Milani, J. Friml, T. Vernoux, C. Godin, O. Hamant, J. Traas, Current Biology 24 (2014) 2335–2342."},"scopus_import":"1","status":"public","publication":"Current Biology","publist_id":"5248","abstract":[{"lang":"eng","text":"To control morphogenesis, molecular regulatory networks have to interfere with the mechanical properties of the individual cells of developing organs and tissues, but how this is achieved is not well known. We study this issue here in the shoot meristem of higher plants, a group of undifferentiated cells where complex changes in growth rates and directions lead to the continuous formation of new organs [1, 2]. Here, we show that the plant hormone auxin plays an important role in this process via a dual, local effect on the extracellular matrix, the cell wall, which determines cell shape. Our study reveals that auxin not only causes a limited reduction in wall stiffness but also directly interferes with wall anisotropy via the regulation of cortical microtubule dynamics. We further show that to induce growth isotropy and organ outgrowth, auxin somehow interferes with the cortical microtubule-ordering activity of a network of proteins, including AUXIN BINDING PROTEIN 1 and KATANIN 1. Numerical simulations further indicate that the induced isotropy is sufficient to amplify the effects of the relatively minor changes in wall stiffness to promote organogenesis and the establishment of new growth axes in a robust manner."}],"intvolume":"        24","department":[{"_id":"JiFr"}],"date_published":"2014-10-06T00:00:00Z","language":[{"iso":"eng"}],"oa":1,"external_id":{"isi":["000342747600031"]},"article_processing_charge":"No","main_file_link":[{"url":"https://hal.archives-ouvertes.fr/hal-01074821","open_access":"1"}]},{"doi":"10.1038/nature13889","_id":"1862","publisher":"Nature Publishing Group","acknowledgement":"We thank R. Dixit for performing complementary experiments, D. W. Ehrhardt and T. Hashimoto for providing the seeds of TUB6–RFP and EB1b–GFP respectively, E. Zazimalova, J. Petrasek and M. Fendrych for discussing the manuscript and J. Leung for text optimization. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP, to J.F.), ANR blanc AuxiWall project (ANR-11-BSV5-0007, to C.P.-R. and L.G.) and the Agency for Innovation by Science and Technology (IWT) (to H.R.). This work benefited from the facilities and expertise of the Imagif Cell Biology platform (http://www.imagif.cnrs.fr), which is supported by the Conseil Général de l’Essonne.","corr_author":"1","title":"Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules","day":"04","year":"2014","article_type":"original","month":"12","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","fulldoi":"https://doi.org/10.1038/nature13889","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"quality_controlled":"1","date_updated":"2025-09-29T13:10:05Z","volume":516,"oa_version":"Submitted Version","isi":1,"scopus_import":"1","publist_id":"5237","status":"public","publication":"Nature","oa":1,"external_id":{"pmid":["25409144"],"isi":["000346310800045"]},"language":[{"iso":"eng"}],"article_processing_charge":"No","ec_funded":1,"intvolume":"       516","abstract":[{"text":"The prominent and evolutionarily ancient role of the plant hormone auxin is the regulation of cell expansion. Cell expansion requires ordered arrangement of the cytoskeleton but molecular mechanisms underlying its regulation by signalling molecules including auxin are unknown. Here we show in the model plant Arabidopsis thaliana that in elongating cells exogenous application of auxin or redistribution of endogenous auxin induces very rapid microtubule re-orientation from transverse to longitudinal, coherent with the inhibition of cell expansion. This fast auxin effect requires auxin binding protein 1 (ABP1) and involves a contribution of downstream signalling components such as ROP6 GTPase, ROP-interactive protein RIC1 and the microtubule-severing protein katanin. These components are required for rapid auxin-and ABP1-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses.","lang":"eng"}],"date_published":"2014-12-04T00:00:00Z","department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"EvBe"}],"pmid":1,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4257754/"}],"type":"journal_article","author":[{"last_name":"Chen","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","full_name":"Chen, Xu","first_name":"Xu"},{"full_name":"Grandont, Laurie","first_name":"Laurie","last_name":"Grandont"},{"full_name":"Li, Hongjiang","orcid":"0000-0001-5039-9660","first_name":"Hongjiang","last_name":"Li","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert"},{"last_name":"Paque","first_name":"Sébastien","full_name":"Paque, Sébastien"},{"full_name":"Abuzeineh, Anas","first_name":"Anas","last_name":"Abuzeineh"},{"full_name":"Rakusova, Hana","first_name":"Hana","last_name":"Rakusova","id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","first_name":"Eva","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"last_name":"Perrot Rechenmann","first_name":"Catherine","full_name":"Perrot Rechenmann, Catherine"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"issue":"729","page":"90 - 93","date_created":"2018-12-11T11:54:25Z","publication_status":"published","project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300","name":"Polarity and subcellular dynamics in plants"}],"citation":{"apa":"Chen, X., Grandont, L., Li, H., Hauschild, R., Paque, S., Abuzeineh, A., … Friml, J. (2014). Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature13889\">https://doi.org/10.1038/nature13889</a>","short":"X. Chen, L. Grandont, H. Li, R. Hauschild, S. Paque, A. Abuzeineh, H. Rakusova, E. Benková, C. Perrot Rechenmann, J. Friml, Nature 516 (2014) 90–93.","ista":"Chen X, Grandont L, Li H, Hauschild R, Paque S, Abuzeineh A, Rakusova H, Benková E, Perrot Rechenmann C, Friml J. 2014. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. Nature. 516(729), 90–93.","ama":"Chen X, Grandont L, Li H, et al. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. <i>Nature</i>. 2014;516(729):90-93. doi:<a href=\"https://doi.org/10.1038/nature13889\">10.1038/nature13889</a>","mla":"Chen, Xu, et al. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” <i>Nature</i>, vol. 516, no. 729, Nature Publishing Group, 2014, pp. 90–93, doi:<a href=\"https://doi.org/10.1038/nature13889\">10.1038/nature13889</a>.","ieee":"X. Chen <i>et al.</i>, “Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules,” <i>Nature</i>, vol. 516, no. 729. Nature Publishing Group, pp. 90–93, 2014.","chicago":"Chen, Xu, Laurie Grandont, Hongjiang Li, Robert Hauschild, Sébastien Paque, Anas Abuzeineh, Hana Rakusova, Eva Benková, Catherine Perrot Rechenmann, and Jiří Friml. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” <i>Nature</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/nature13889\">https://doi.org/10.1038/nature13889</a>."}},{"oa_version":"Submitted Version","isi":1,"fulldoi":"https://doi.org/10.1073/pnas.1324264111","volume":111,"date_updated":"2025-09-29T13:05:44Z","month":"02","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","acknowledgement":"This work was supported by grants from the Research Foundation-Flanders (Odysseus).","_id":"1893","publisher":"National Academy of Sciences","doi":"10.1073/pnas.1324264111","year":"2014","day":"18","title":"SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis","corr_author":"1","project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"publication_status":"published","citation":{"chicago":"Marhavá, Petra, Sibylle Hirsch, Elena Feraru, Ricardo Tejos, Ringo Van Wijk, Tom Viaene, Mareike Heilmann, et al. “SAC Phosphoinositide Phosphatases at the Tonoplast Mediate Vacuolar Function in Arabidopsis.” <i>PNAS</i>. National Academy of Sciences, 2014. <a href=\"https://doi.org/10.1073/pnas.1324264111\">https://doi.org/10.1073/pnas.1324264111</a>.","mla":"Marhavá, Petra, et al. “SAC Phosphoinositide Phosphatases at the Tonoplast Mediate Vacuolar Function in Arabidopsis.” <i>PNAS</i>, vol. 111, no. 7, National Academy of Sciences, 2014, pp. 2818–23, doi:<a href=\"https://doi.org/10.1073/pnas.1324264111\">10.1073/pnas.1324264111</a>.","ieee":"P. Marhavá <i>et al.</i>, “SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis,” <i>PNAS</i>, vol. 111, no. 7. National Academy of Sciences, pp. 2818–2823, 2014.","ama":"Marhavá P, Hirsch S, Feraru E, et al. SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis. <i>PNAS</i>. 2014;111(7):2818-2823. doi:<a href=\"https://doi.org/10.1073/pnas.1324264111\">10.1073/pnas.1324264111</a>","ista":"Marhavá P, Hirsch S, Feraru E, Tejos R, Van Wijk R, Viaene T, Heilmann M, Lerche J, De Rycke R, Feraru M, Grones P, Van Montagu M, Heilmann I, Munnik T, Friml J. 2014. SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis. PNAS. 111(7), 2818–2823.","apa":"Marhavá, P., Hirsch, S., Feraru, E., Tejos, R., Van Wijk, R., Viaene, T., … Friml, J. (2014). SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1324264111\">https://doi.org/10.1073/pnas.1324264111</a>","short":"P. Marhavá, S. Hirsch, E. Feraru, R. Tejos, R. Van Wijk, T. Viaene, M. Heilmann, J. Lerche, R. De Rycke, M. Feraru, P. Grones, M. Van Montagu, I. Heilmann, T. Munnik, J. Friml, PNAS 111 (2014) 2818–2823."},"date_created":"2018-12-11T11:54:34Z","page":"2818 - 2823","issue":"7","author":[{"last_name":"Nováková","id":"44E59624-F248-11E8-B48F-1D18A9856A87","full_name":"Nováková, Petra","first_name":"Petra"},{"last_name":"Hirsch","full_name":"Hirsch, Sibylle","first_name":"Sibylle"},{"full_name":"Feraru, Elena","first_name":"Elena","last_name":"Feraru"},{"last_name":"Tejos","full_name":"Tejos, Ricardo","first_name":"Ricardo"},{"first_name":"Ringo","full_name":"Van Wijk, Ringo","last_name":"Van Wijk"},{"full_name":"Viaene, Tom","first_name":"Tom","last_name":"Viaene"},{"full_name":"Heilmann, Mareike","first_name":"Mareike","last_name":"Heilmann"},{"full_name":"Lerche, Jennifer","first_name":"Jennifer","last_name":"Lerche"},{"full_name":"De Rycke, Riet","first_name":"Riet","last_name":"De Rycke"},{"first_name":"Mugurel","full_name":"Feraru, Mugurel","last_name":"Feraru"},{"last_name":"Grones","id":"399876EC-F248-11E8-B48F-1D18A9856A87","full_name":"Grones, Peter","first_name":"Peter"},{"first_name":"Marc","full_name":"Van Montagu, Marc","last_name":"Van Montagu"},{"full_name":"Heilmann, Ingo","first_name":"Ingo","last_name":"Heilmann"},{"full_name":"Munnik, Teun","first_name":"Teun","last_name":"Munnik"},{"first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"type":"journal_article","department":[{"_id":"JiFr"}],"date_published":"2014-02-18T00:00:00Z","abstract":[{"text":"Phosphatidylinositol (PtdIns) is a structural phospholipid that can be phosphorylated into various lipid signaling molecules, designated polyphosphoinositides (PPIs). The reversible phosphorylation of PPIs on the 3, 4, or 5 position of inositol is performed by a set of organelle-specific kinases and phosphatases, and the characteristic head groups make these molecules ideal for regulating biological processes in time and space. In yeast and mammals, PtdIns3P and PtdIns(3,5)P2 play crucial roles in trafficking toward the lytic compartments, whereas the role in plants is not yet fully understood. Here we identified the role of a land plant-specific subgroup of PPI phosphatases, the suppressor of actin 2 (SAC2) to SAC5, during vacuolar trafficking and morphogenesis in Arabidopsis thaliana. SAC2-SAC5 localize to the tonoplast along with PtdIns3P, the presumable product of their activity. In SAC gain- and loss-of-function mutants, the levels of PtdIns monophosphates and bisphosphates were changed, with opposite effects on the morphology of storage and lytic vacuoles, and the trafficking toward the vacuoles was defective. Moreover, multiple sac knockout mutants had an increased number of smaller storage and lytic vacuoles, whereas extralarge vacuoles were observed in the overexpression lines, correlating with various growth and developmental defects. The fragmented vacuolar phenotype of sac mutants could be mimicked by treating wild-type seedlings with PtdIns(3,5)P2, corroborating that this PPI is important for vacuole morphology. Taken together, these results provide evidence that PPIs, together with their metabolic enzymes SAC2-SAC5, are crucial for vacuolar trafficking and for vacuolar morphology and function in plants.","lang":"eng"}],"intvolume":"       111","ec_funded":1,"article_processing_charge":"No","language":[{"iso":"eng"}],"oa":1,"external_id":{"isi":["000331396500082"]},"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932866/","open_access":"1"}],"scopus_import":"1","publication":"PNAS","publist_id":"5202","status":"public"},{"isi":1,"oa_version":"Submitted Version","date_updated":"2025-09-29T13:04:06Z","volume":26,"fulldoi":"https://doi.org/10.1105/tpc.114.125880","month":"07","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","title":"Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis","day":"01","year":"2014","doi":"10.1105/tpc.114.125880","publisher":"American Society of Plant Biologists","_id":"1897","acknowledgement":"This work was supported by the Odysseus Program of the Research Foundation-Flanders (J.F.).","citation":{"ieee":"S. Naramoto <i>et al.</i>, “Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis,” <i>Plant Cell</i>, vol. 26, no. 7. American Society of Plant Biologists, pp. 3062–3076, 2014.","ama":"Naramoto S, Otegui M, Kutsuna N, et al. Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis. <i>Plant Cell</i>. 2014;26(7):3062-3076. doi:<a href=\"https://doi.org/10.1105/tpc.114.125880\">10.1105/tpc.114.125880</a>","chicago":"Naramoto, Satoshi, Marisa Otegui, Natsumaro Kutsuna, Riet De Rycke, Tomoko Dainobu, Michael Karampelias, Masaru Fujimoto, et al. “Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis.” <i>Plant Cell</i>. American Society of Plant Biologists, 2014. <a href=\"https://doi.org/10.1105/tpc.114.125880\">https://doi.org/10.1105/tpc.114.125880</a>.","mla":"Naramoto, Satoshi, et al. “Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis.” <i>Plant Cell</i>, vol. 26, no. 7, American Society of Plant Biologists, 2014, pp. 3062–76, doi:<a href=\"https://doi.org/10.1105/tpc.114.125880\">10.1105/tpc.114.125880</a>.","ista":"Naramoto S, Otegui M, Kutsuna N, De Rycke R, Dainobu T, Karampelias M, Fujimoto M, Feraru E, Miki D, Fukuda H, Nakano A, Friml J. 2014. Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis. Plant Cell. 26(7), 3062–3076.","short":"S. Naramoto, M. Otegui, N. Kutsuna, R. De Rycke, T. Dainobu, M. Karampelias, M. Fujimoto, E. Feraru, D. Miki, H. Fukuda, A. Nakano, J. Friml, Plant Cell 26 (2014) 3062–3076.","apa":"Naramoto, S., Otegui, M., Kutsuna, N., De Rycke, R., Dainobu, T., Karampelias, M., … Friml, J. (2014). Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.114.125880\">https://doi.org/10.1105/tpc.114.125880</a>"},"publication_status":"published","type":"journal_article","author":[{"last_name":"Naramoto","full_name":"Naramoto, Satoshi","first_name":"Satoshi"},{"first_name":"Marisa","full_name":"Otegui, Marisa","last_name":"Otegui"},{"last_name":"Kutsuna","full_name":"Kutsuna, Natsumaro","first_name":"Natsumaro"},{"first_name":"Riet","full_name":"De Rycke, Riet","last_name":"De Rycke"},{"last_name":"Dainobu","full_name":"Dainobu, Tomoko","first_name":"Tomoko"},{"last_name":"Karampelias","full_name":"Karampelias, Michael","first_name":"Michael"},{"last_name":"Fujimoto","full_name":"Fujimoto, Masaru","first_name":"Masaru"},{"last_name":"Feraru","first_name":"Elena","full_name":"Feraru, Elena"},{"last_name":"Miki","full_name":"Miki, Daisuke","first_name":"Daisuke"},{"last_name":"Fukuda","full_name":"Fukuda, Hiroo","first_name":"Hiroo"},{"last_name":"Nakano","first_name":"Akihiko","full_name":"Nakano, Akihiko"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","first_name":"Jirí"}],"issue":"7","page":"3062 - 3076","date_created":"2018-12-11T11:54:36Z","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145132/"}],"oa":1,"external_id":{"isi":["000342076200027"]},"language":[{"iso":"eng"}],"article_processing_charge":"No","intvolume":"        26","abstract":[{"text":"GNOM is one of the most characterized membrane trafficking regulators in plants, with crucial roles in development. GNOM encodes an ARF-guanine nucleotide exchange factor (ARF-GEF) that activates small GTPases of the ARF (ADP ribosylation factor) class to mediate vesicle budding at endomembranes. The crucial role of GNOM in recycling of PIN auxin transporters and other proteins to the plasma membrane was identified in studies using the ARF-GEF inhibitor brefeldin A (BFA). GNOM, the most prominent regulator of recycling in plants, has been proposed to act and localize at so far elusive recycling endosomes. Here, we report the GNOM localization in context of its cellular function in Arabidopsis thaliana. State-of-the-art imaging, pharmacological interference, and ultrastructure analysis show that GNOM predominantly localizes to Golgi apparatus. Super-resolution confocal live imaging microscopy identified GNOM and its closest homolog GNOM-like 1 at distinct subdomains on Golgi cisternae. Short-term BFA treatment stabilizes GNOM at the Golgi apparatus, whereas prolonged exposures results in GNOM translocation to trans-Golgi network (TGN)/early endosomes (EEs). Malformed TGN/EE in gnom mutants suggests a role for GNOM in maintaining TGN/EE function. Our results redefine the subcellular action of GNOM and reevaluate the identity and function of recycling endosomes in plants.","lang":"eng"}],"date_published":"2014-07-01T00:00:00Z","department":[{"_id":"JiFr"}],"publication":"Plant Cell","publist_id":"5199","status":"public","scopus_import":"1"},{"month":"02","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","title":"WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis","year":"2014","day":"01","publisher":"Oxford University Press","acknowledgement":"This work was supported by funding from the projects CZ.1.07/2.3.00/20.0043 and CZ.1.05/1.1.00/02.0068 (to CEITEC, Central European Institute of Technology) and the Odysseus program of the Research Foundation-Flanders to J.F\r\n","_id":"1901","doi":"10.1093/mp/sst118","isi":1,"oa_version":"None","date_updated":"2025-09-29T12:30:56Z","volume":7,"fulldoi":"https://doi.org/10.1093/mp/sst118","article_processing_charge":"No","external_id":{"isi":["000330841400003"]},"language":[{"iso":"eng"}],"date_published":"2014-02-01T00:00:00Z","department":[{"_id":"JiFr"}],"intvolume":"         7","abstract":[{"text":"In plants, the patterning of stem cell-enriched meristems requires a graded auxin response maximum that emerges from the concerted action of polar auxin transport, auxin biosynthesis, auxin metabolism, and cellular auxin response machinery. However, mechanisms underlying this auxin response maximum-mediated root stem cell maintenance are not fully understood. Here, we present unexpected evidence that WUSCHEL-RELATED HOMEOBOX 5 (WOX5) transcription factor modulates expression of auxin biosynthetic genes in the quiescent center (QC) of the root and thus provides a robust mechanism for the maintenance of auxin response maximum in the root tip. This WOX5 action is balanced through the activity of indole-3-acetic acid 17 (IAA17) auxin response repressor. Our combined genetic, cell biology, and computational modeling studies revealed a previously uncharacterized feedback loop linking WOX5-mediated auxin production to IAA17-dependent repression of auxin responses. This WOX5-IAA17 feedback circuit further assures the maintenance of auxin response maximum in the root tip and thereby contributes to the maintenance of distal stem cell (DSC) populations. Our experimental studies and in silico computer simulations both demonstrate that the WOX5-IAA17 feedback circuit is essential for the maintenance of auxin gradient in the root tip and the auxin-mediated root DSC differentiation.","lang":"eng"}],"status":"public","publication":"Molecular Plant","publist_id":"5194","scopus_import":"1","citation":{"chicago":"Tian, Huiyu, Krzysztof T Wabnik, Tiantian Niu, Hongjiang Li, Qianqian Yu, Stephan Pollmann, Steffen Vanneste, et al. “WOX5-IAA17 Feedback Circuit-Mediated Cellular Auxin Response Is Crucial for the Patterning of Root Stem Cell Niches in Arabidopsis.” <i>Molecular Plant</i>. Oxford University Press, 2014. <a href=\"https://doi.org/10.1093/mp/sst118\">https://doi.org/10.1093/mp/sst118</a>.","mla":"Tian, Huiyu, et al. “WOX5-IAA17 Feedback Circuit-Mediated Cellular Auxin Response Is Crucial for the Patterning of Root Stem Cell Niches in Arabidopsis.” <i>Molecular Plant</i>, vol. 7, no. 2, Oxford University Press, 2014, pp. 277–89, doi:<a href=\"https://doi.org/10.1093/mp/sst118\">10.1093/mp/sst118</a>.","ama":"Tian H, Wabnik KT, Niu T, et al. WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis. <i>Molecular Plant</i>. 2014;7(2):277-289. doi:<a href=\"https://doi.org/10.1093/mp/sst118\">10.1093/mp/sst118</a>","ieee":"H. Tian <i>et al.</i>, “WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis,” <i>Molecular Plant</i>, vol. 7, no. 2. Oxford University Press, pp. 277–289, 2014.","ista":"Tian H, Wabnik KT, Niu T, Li H, Yu Q, Pollmann S, Vanneste S, Govaerts W, Rolčík J, Geisler M, Friml J, Ding Z. 2014. WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis. Molecular Plant. 7(2), 277–289.","apa":"Tian, H., Wabnik, K. T., Niu, T., Li, H., Yu, Q., Pollmann, S., … Ding, Z. (2014). WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in arabidopsis. <i>Molecular Plant</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/mp/sst118\">https://doi.org/10.1093/mp/sst118</a>","short":"H. Tian, K.T. Wabnik, T. Niu, H. Li, Q. Yu, S. Pollmann, S. Vanneste, W. Govaerts, J. Rolčík, M. Geisler, J. Friml, Z. Ding, Molecular Plant 7 (2014) 277–289."},"publication_status":"published","issue":"2","author":[{"last_name":"Tian","full_name":"Tian, Huiyu","first_name":"Huiyu"},{"full_name":"Wabnik, Krzysztof T","first_name":"Krzysztof T","last_name":"Wabnik"},{"last_name":"Niu","full_name":"Niu, Tiantian","first_name":"Tiantian"},{"last_name":"Li","first_name":"Hongjiang","full_name":"Li, Hongjiang"},{"last_name":"Yu","first_name":"Qianqian","full_name":"Yu, Qianqian"},{"first_name":"Stephan","full_name":"Pollmann, Stephan","last_name":"Pollmann"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"last_name":"Govaerts","first_name":"Willy","full_name":"Govaerts, Willy"},{"full_name":"Rolčík, Jakub","first_name":"Jakub","last_name":"Rolčík"},{"last_name":"Geisler","full_name":"Geisler, Markus","first_name":"Markus"},{"first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"full_name":"Ding, Zhaojun","first_name":"Zhaojun","last_name":"Ding"}],"type":"journal_article","date_created":"2018-12-11T11:54:37Z","page":"277 - 289"},{"year":"2014","day":"06","title":"Plant biology: Gatekeepers of the road to protein perdition","corr_author":"1","_id":"1914","publisher":"Cell Press","doi":"10.1016/j.cub.2013.11.019","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"01","volume":24,"date_updated":"2025-09-29T12:23:35Z","quality_controlled":"1","fulldoi":"https://doi.org/10.1016/j.cub.2013.11.019","isi":1,"oa_version":"None","publication":"Current Biology","publist_id":"5180","status":"public","scopus_import":"1","date_published":"2014-01-06T00:00:00Z","department":[{"_id":"JiFr"}],"abstract":[{"text":"Targeting membrane proteins for degradation requires the sequential action of ESCRT sub-complexes ESCRT-0 to ESCRT-III. Although this machinery is generally conserved among kingdoms, plants lack the essential ESCRT-0 components. A new report closes this gap by identifying a novel protein family that substitutes for ESCRT-0 function in plants.","lang":"eng"}],"intvolume":"        24","article_processing_charge":"No","language":[{"iso":"eng"}],"external_id":{"isi":["000329501400011"]},"date_created":"2018-12-11T11:54:41Z","page":"R27 - R29","issue":"1","author":[{"full_name":"Sauer, Michael","first_name":"Michael","last_name":"Sauer"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"type":"journal_article","citation":{"ista":"Sauer M, Friml J. 2014. Plant biology: Gatekeepers of the road to protein perdition. Current Biology. 24(1), R27–R29.","mla":"Sauer, Michael, and Jiří Friml. “Plant Biology: Gatekeepers of the Road to Protein Perdition.” <i>Current Biology</i>, vol. 24, no. 1, Cell Press, 2014, pp. R27–29, doi:<a href=\"https://doi.org/10.1016/j.cub.2013.11.019\">10.1016/j.cub.2013.11.019</a>.","chicago":"Sauer, Michael, and Jiří Friml. “Plant Biology: Gatekeepers of the Road to Protein Perdition.” <i>Current Biology</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cub.2013.11.019\">https://doi.org/10.1016/j.cub.2013.11.019</a>.","ama":"Sauer M, Friml J. Plant biology: Gatekeepers of the road to protein perdition. <i>Current Biology</i>. 2014;24(1):R27-R29. doi:<a href=\"https://doi.org/10.1016/j.cub.2013.11.019\">10.1016/j.cub.2013.11.019</a>","ieee":"M. Sauer and J. Friml, “Plant biology: Gatekeepers of the road to protein perdition,” <i>Current Biology</i>, vol. 24, no. 1. Cell Press, pp. R27–R29, 2014.","short":"M. Sauer, J. Friml, Current Biology 24 (2014) R27–R29.","apa":"Sauer, M., &#38; Friml, J. (2014). Plant biology: Gatekeepers of the road to protein perdition. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2013.11.019\">https://doi.org/10.1016/j.cub.2013.11.019</a>"},"publication_status":"published"},{"ec_funded":1,"article_processing_charge":"No","language":[{"iso":"eng"}],"external_id":{"pmid":["24450654"],"isi":["000333444400034"]},"department":[{"_id":"JiFr"}],"date_published":"2014-02-01T00:00:00Z","abstract":[{"text":"ROPs (Rho of plants) belong to a large family of plant-specific Rho-like small GTPases that function as essential molecular switches to control diverse cellular processes including cytoskeleton organization, cell polarization, cytokinesis, cell differentiation and vesicle trafficking. Although the machineries of vesicle trafficking and cell polarity in plants have been individually well addressed, how ROPs co-ordinate those processes is still largely unclear. Recent progress has been made towards an understanding of the coordination of ROP signalling and trafficking of PIN (PINFORMED) transporters for the plant hormone auxin in both root and leaf pavement cells. PIN transporters constantly shuttle between the endosomal compartments and the polar plasma membrane domains, therefore the modulation of PIN-dependent auxin transport between cells is a main developmental output of ROP-regulated vesicle trafficking. The present review focuses on these cellular mechanisms, especially the integration of ROP-based vesicle trafficking and plant cell polarity.","lang":"eng"}],"intvolume":"        42","pmid":1,"scopus_import":"1","publication":"Biochemical Society Transactions","publist_id":"5179","status":"public","publication_status":"published","project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300"}],"citation":{"ista":"Chen X, Friml J. 2014. Rho-GTPase-regulated vesicle trafficking in plant cell polarity. Biochemical Society Transactions. 42(1), 212–218.","ieee":"X. Chen and J. Friml, “Rho-GTPase-regulated vesicle trafficking in plant cell polarity,” <i>Biochemical Society Transactions</i>, vol. 42, no. 1. Portland Press, pp. 212–218, 2014.","chicago":"Chen, Xu, and Jiří Friml. “Rho-GTPase-Regulated Vesicle Trafficking in Plant Cell Polarity.” <i>Biochemical Society Transactions</i>. Portland Press, 2014. <a href=\"https://doi.org/10.1042/BST20130269\">https://doi.org/10.1042/BST20130269</a>.","ama":"Chen X, Friml J. Rho-GTPase-regulated vesicle trafficking in plant cell polarity. <i>Biochemical Society Transactions</i>. 2014;42(1):212-218. doi:<a href=\"https://doi.org/10.1042/BST20130269\">10.1042/BST20130269</a>","mla":"Chen, Xu, and Jiří Friml. “Rho-GTPase-Regulated Vesicle Trafficking in Plant Cell Polarity.” <i>Biochemical Society Transactions</i>, vol. 42, no. 1, Portland Press, 2014, pp. 212–18, doi:<a href=\"https://doi.org/10.1042/BST20130269\">10.1042/BST20130269</a>.","short":"X. Chen, J. Friml, Biochemical Society Transactions 42 (2014) 212–218.","apa":"Chen, X., &#38; Friml, J. (2014). Rho-GTPase-regulated vesicle trafficking in plant cell polarity. <i>Biochemical Society Transactions</i>. Portland Press. <a href=\"https://doi.org/10.1042/BST20130269\">https://doi.org/10.1042/BST20130269</a>"},"issue":"1","author":[{"first_name":"Xu","full_name":"Chen, Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","last_name":"Chen"},{"first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"type":"journal_article","date_created":"2018-12-11T11:54:41Z","page":"212 - 218","article_type":"original","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"02","_id":"1915","acknowledgement":"This work was supported by the European Research Council [project ERC-2011-StG-20101109-PSDP], Central European Institute of Technology (CEITEC) [grant number CZ.1.05/1.1.00/02.0068], European Social Fund [grant number CZ.1.07/2.3.00/20.0043] and the Czec","publisher":"Portland Press","doi":"10.1042/BST20130269","title":"Rho-GTPase-regulated vesicle trafficking in plant cell polarity","corr_author":"1","year":"2014","day":"01","oa_version":"None","isi":1,"fulldoi":"https://doi.org/10.1042/BST20130269","publication_identifier":{"issn":["0300-5127"],"eissn":["1470-8752"]},"quality_controlled":"1","date_updated":"2025-09-29T12:22:54Z","volume":42},{"oa_version":"Submitted Version","isi":1,"fulldoi":"https://doi.org/10.1126/science.1245125","quality_controlled":"1","date_updated":"2025-09-29T12:20:10Z","volume":343,"article_type":"original","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"02","acknowledgement":"Supported by the intramural research program of the National Institute of Arthritis and Musculoskeletal and Skin Diseases and by its Laboratory Animal Care and Use Section and Flow Cytometry Group, Office of Science and Technology","_id":"1917","publisher":"American Association for the Advancement of Science","doi":"10.1126/science.1245125","title":"Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling","year":"2014","day":"28","publication_status":"published","citation":{"apa":"Xu, T., Dai, N., Chen, J., Nagawa, S., Cao, M., Li, H., … Yang, Z. (2014). Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1245125\">https://doi.org/10.1126/science.1245125</a>","short":"T. Xu, N. Dai, J. Chen, S. Nagawa, M. Cao, H. Li, Z. Zhou, X. Chen, R. De Rycke, H. Rakusová, W. Wang, A. Jones, J. Friml, S. Patterson, A. Bleecker, Z. Yang, Science 343 (2014) 1025–1028.","chicago":"Xu, Tongda, Ning Dai, Jisheng Chen, Shingo Nagawa, Min Cao, Hongjiang Li, Zimin Zhou, et al. “Cell Surface ABP1-TMK Auxin Sensing Complex Activates ROP GTPase Signaling.” <i>Science</i>. American Association for the Advancement of Science, 2014. <a href=\"https://doi.org/10.1126/science.1245125\">https://doi.org/10.1126/science.1245125</a>.","mla":"Xu, Tongda, et al. “Cell Surface ABP1-TMK Auxin Sensing Complex Activates ROP GTPase Signaling.” <i>Science</i>, vol. 343, no. 6174, American Association for the Advancement of Science, 2014, pp. 1025–28, doi:<a href=\"https://doi.org/10.1126/science.1245125\">10.1126/science.1245125</a>.","ama":"Xu T, Dai N, Chen J, et al. Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. <i>Science</i>. 2014;343(6174):1025-1028. doi:<a href=\"https://doi.org/10.1126/science.1245125\">10.1126/science.1245125</a>","ieee":"T. Xu <i>et al.</i>, “Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling,” <i>Science</i>, vol. 343, no. 6174. American Association for the Advancement of Science, pp. 1025–1028, 2014.","ista":"Xu T, Dai N, Chen J, Nagawa S, Cao M, Li H, Zhou Z, Chen X, De Rycke R, Rakusová H, Wang W, Jones A, Friml J, Patterson S, Bleecker A, Yang Z. 2014. Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. Science. 343(6174), 1025–1028."},"author":[{"last_name":"Xu","full_name":"Xu, Tongda","first_name":"Tongda"},{"first_name":"Ning","full_name":"Dai, Ning","last_name":"Dai"},{"last_name":"Chen","first_name":"Jisheng","full_name":"Chen, Jisheng"},{"first_name":"Shingo","full_name":"Nagawa, Shingo","last_name":"Nagawa"},{"last_name":"Cao","full_name":"Cao, Min","first_name":"Min"},{"id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","last_name":"Li","first_name":"Hongjiang","full_name":"Li, Hongjiang","orcid":"0000-0001-5039-9660"},{"last_name":"Zhou","first_name":"Zimin","full_name":"Zhou, Zimin"},{"id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","last_name":"Chen","first_name":"Xu","full_name":"Chen, Xu"},{"first_name":"Riet","full_name":"De Rycke, Riet","last_name":"De Rycke"},{"last_name":"Rakusová","full_name":"Rakusová, Hana","first_name":"Hana"},{"last_name":"Wang","full_name":"Wang, Wen","first_name":"Wen"},{"first_name":"Alan","full_name":"Jones, Alan","last_name":"Jones"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"last_name":"Patterson","full_name":"Patterson, Sara","first_name":"Sara"},{"full_name":"Bleecker, Anthony","first_name":"Anthony","last_name":"Bleecker"},{"last_name":"Yang","first_name":"Zhenbiao","full_name":"Yang, Zhenbiao"}],"issue":"6174","type":"journal_article","date_created":"2018-12-11T11:54:42Z","page":"1025 - 1028","article_processing_charge":"No","language":[{"iso":"eng"}],"external_id":{"pmid":["24578577"],"isi":["000332309600046"]},"oa":1,"date_published":"2014-02-28T00:00:00Z","department":[{"_id":"JiFr"}],"intvolume":"       343","abstract":[{"text":"Auxin-binding protein 1 (ABP1) was discovered nearly 40 years ago and was shown to be essential for plant development and morphogenesis, but its mode of action remains unclear. Here, we report that the plasma membrane-localized transmembrane kinase (TMK) receptor-like kinases interact with ABP1 and transduce auxin signal to activate plasma membrane-associated ROPs [Rho-like guanosine triphosphatases (GTPase) from plants], leading to changes in the cytoskeleton and the shape of leaf pavement cells in Arabidopsis. The interaction between ABP1 and TMK at the cell surface is induced by auxin and requires ABP1 sensing of auxin. These findings show that TMK proteins and ABP1 form a cell surface auxin perception complex that activates ROP signaling pathways, regulating nontranscriptional cytoplasmic responses and associated fundamental processes.","lang":"eng"}],"pmid":1,"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166562/"}],"scopus_import":"1","publist_id":"5177","publication":"Science","status":"public"},{"scopus_import":"1","publication":"Plant Cell","publist_id":"5173","status":"public","abstract":[{"lang":"eng","text":"Cell polarity manifested by asymmetric distribution of cargoes, such as receptors and transporters, within the plasma membrane (PM) is crucial for essential functions in multicellular organisms. In plants, cell polarity (re)establishment is intimately linked to patterning processes. Despite the importance of cell polarity, its underlying mechanisms are still largely unknown, including the definition and distinctiveness of the polar domains within the PM. Here, we show in Arabidopsis thaliana that the signaling membrane components, the phosphoinositides phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4, 5-bisphosphate [PtdIns(4, 5)P2] as well as PtdIns4P 5-kinases mediating their interconversion, are specifically enriched at apical and basal polar plasma membrane domains. The PtdIns4P 5-kinases PIP5K1 and PIP5K2 are redundantly required for polar localization of specifically apical and basal cargoes, such as PIN-FORMED transporters for the plant hormone auxin. As a consequence of the polarity defects, instructive auxin gradients as well as embryonic and postembryonic patterning are severely compromised. Furthermore, auxin itself regulates PIP5K transcription and PtdIns4P and PtdIns(4, 5)P2 levels, in particular their association with polar PM domains. Our results provide insight into the polar domain-delineating mechanisms in plant cells that depend on apical and basal distribution of membrane lipids and are essential for embryonic and postembryonic patterning."}],"intvolume":"        26","department":[{"_id":"JiFr"}],"date_published":"2014-05-01T00:00:00Z","language":[{"iso":"eng"}],"oa":1,"external_id":{"isi":["000338771700027"]},"ec_funded":1,"article_processing_charge":"No","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079372/"}],"page":"2114 - 2128","date_created":"2018-12-11T11:54:43Z","type":"journal_article","author":[{"last_name":"Tejos","first_name":"Ricardo","full_name":"Tejos, Ricardo"},{"last_name":"Sauer","full_name":"Sauer, Michael","first_name":"Michael"},{"full_name":"Vanneste, Steffen","first_name":"Steffen","last_name":"Vanneste"},{"last_name":"Palacios-Gomez","full_name":"Palacios-Gomez, MiriamPalacios ","first_name":"MiriamPalacios "},{"first_name":"Hongjiang","orcid":"0000-0001-5039-9660","full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","last_name":"Li"},{"full_name":"Heilmann, Mareike","first_name":"Mareike","last_name":"Heilmann"},{"first_name":"Ringo","full_name":"Van Wijk, Ringo","last_name":"Van Wijk"},{"last_name":"Vermeer","full_name":"Vermeer, Joop","first_name":"Joop"},{"full_name":"Heilmann, Ingo","first_name":"Ingo","last_name":"Heilmann"},{"last_name":"Munnik","full_name":"Munnik, Teun","first_name":"Teun"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"issue":"5","project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300"}],"publication_status":"published","citation":{"apa":"Tejos, R., Sauer, M., Vanneste, S., Palacios-Gomez, M., Li, H., Heilmann, M., … Friml, J. (2014). Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.114.126185\">https://doi.org/10.1105/tpc.114.126185</a>","short":"R. Tejos, M. Sauer, S. Vanneste, M. Palacios-Gomez, H. Li, M. Heilmann, R. Van Wijk, J. Vermeer, I. Heilmann, T. Munnik, J. Friml, Plant Cell 26 (2014) 2114–2128.","ista":"Tejos R, Sauer M, Vanneste S, Palacios-Gomez M, Li H, Heilmann M, Van Wijk R, Vermeer J, Heilmann I, Munnik T, Friml J. 2014. Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis. Plant Cell. 26(5), 2114–2128.","mla":"Tejos, Ricardo, et al. “Bipolar Plasma Membrane Distribution of Phosphoinositides and Their Requirement for Auxin-Mediated Cell Polarity and Patterning in Arabidopsis.” <i>Plant Cell</i>, vol. 26, no. 5, American Society of Plant Biologists, 2014, pp. 2114–28, doi:<a href=\"https://doi.org/10.1105/tpc.114.126185\">10.1105/tpc.114.126185</a>.","chicago":"Tejos, Ricardo, Michael Sauer, Steffen Vanneste, MiriamPalacios  Palacios-Gomez, Hongjiang Li, Mareike Heilmann, Ringo Van Wijk, et al. “Bipolar Plasma Membrane Distribution of Phosphoinositides and Their Requirement for Auxin-Mediated Cell Polarity and Patterning in Arabidopsis.” <i>Plant Cell</i>. American Society of Plant Biologists, 2014. <a href=\"https://doi.org/10.1105/tpc.114.126185\">https://doi.org/10.1105/tpc.114.126185</a>.","ama":"Tejos R, Sauer M, Vanneste S, et al. Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis. <i>Plant Cell</i>. 2014;26(5):2114-2128. doi:<a href=\"https://doi.org/10.1105/tpc.114.126185\">10.1105/tpc.114.126185</a>","ieee":"R. Tejos <i>et al.</i>, “Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis,” <i>Plant Cell</i>, vol. 26, no. 5. American Society of Plant Biologists, pp. 2114–2128, 2014."},"doi":"10.1105/tpc.114.126185","publisher":"American Society of Plant Biologists","_id":"1921","acknowledgement":"This work was supported by grants from the Odysseus program of the Research Foundation-Flanders (to J.F.).","day":"01","year":"2014","corr_author":"1","title":"Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"05","fulldoi":"https://doi.org/10.1105/tpc.114.126185","volume":26,"date_updated":"2025-09-29T12:17:33Z","oa_version":"Submitted Version","isi":1},{"title":"Auxin transport and activity regulate stomatal patterning and development","day":"27","year":"2014","doi":"10.1038/ncomms4090","_id":"1924","publisher":"Nature Publishing Group","month":"01","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2025-09-29T12:15:23Z","volume":5,"fulldoi":"https://doi.org/10.1038/ncomms4090","quality_controlled":"1","isi":1,"oa_version":"None","publist_id":"5170","publication":"Nature Communications","status":"public","scopus_import":"1","external_id":{"isi":["000331084200032"]},"language":[{"iso":"eng"}],"article_processing_charge":"No","intvolume":"         5","abstract":[{"lang":"eng","text":"Stomata are two-celled valves that control epidermal pores whose spacing optimizes shoot-atmosphere gas exchange. They develop from protodermal cells after unequal divisions followed by an equal division and differentiation. The concentration of the hormone auxin, a master plant developmental regulator, is tightly controlled in time and space, but its role, if any, in stomatal formation is obscure. Here dynamic changes of auxin activity during stomatal development are monitored using auxin input (DII-VENUS) and output (DR5:VENUS) markers by time-lapse imaging. A decrease in auxin levels in the smaller daughter cell after unequal division presages the acquisition of a guard mother cell fate whose equal division produces the two guard cells. Thus, stomatal patterning requires auxin pathway control of stem cell compartment size, as well as auxin depletion that triggers a developmental switch from unequal to equal division."}],"date_published":"2014-01-27T00:00:00Z","department":[{"_id":"JiFr"}],"type":"journal_article","author":[{"last_name":"Le","first_name":"Jie","full_name":"Le, Jie"},{"last_name":"Liu","first_name":"Xuguang","full_name":"Liu, Xuguang"},{"first_name":"Kezhen","full_name":"Yang, Kezhen","last_name":"Yang"},{"full_name":"Chen, Xiaolan","first_name":"Xiaolan","last_name":"Chen"},{"first_name":"Lingling","full_name":"Zhu, Lingling","last_name":"Zhu"},{"last_name":"Wang","first_name":"Hongzhe","full_name":"Wang, Hongzhe"},{"last_name":"Wang","full_name":"Wang, Ming","first_name":"Ming"},{"first_name":"Steffen","full_name":"Vanneste, Steffen","last_name":"Vanneste"},{"last_name":"Morita","first_name":"Miyo","full_name":"Morita, Miyo"},{"last_name":"Tasaka","full_name":"Tasaka, Masao","first_name":"Masao"},{"full_name":"Ding, Zhaojun","first_name":"Zhaojun","last_name":"Ding"},{"full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Tom","full_name":"Beeckman, Tom","last_name":"Beeckman"},{"full_name":"Sack, Fred","first_name":"Fred","last_name":"Sack"}],"date_created":"2018-12-11T11:54:44Z","article_number":"3090","citation":{"ista":"Le J, Liu X, Yang K, Chen X, Zhu L, Wang H, Wang M, Vanneste S, Morita M, Tasaka M, Ding Z, Friml J, Beeckman T, Sack F. 2014. Auxin transport and activity regulate stomatal patterning and development. Nature Communications. 5, 3090.","ama":"Le J, Liu X, Yang K, et al. Auxin transport and activity regulate stomatal patterning and development. <i>Nature Communications</i>. 2014;5. doi:<a href=\"https://doi.org/10.1038/ncomms4090\">10.1038/ncomms4090</a>","mla":"Le, Jie, et al. “Auxin Transport and Activity Regulate Stomatal Patterning and Development.” <i>Nature Communications</i>, vol. 5, 3090, Nature Publishing Group, 2014, doi:<a href=\"https://doi.org/10.1038/ncomms4090\">10.1038/ncomms4090</a>.","ieee":"J. Le <i>et al.</i>, “Auxin transport and activity regulate stomatal patterning and development,” <i>Nature Communications</i>, vol. 5. Nature Publishing Group, 2014.","chicago":"Le, Jie, Xuguang Liu, Kezhen Yang, Xiaolan Chen, Lingling Zhu, Hongzhe Wang, Ming Wang, et al. “Auxin Transport and Activity Regulate Stomatal Patterning and Development.” <i>Nature Communications</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/ncomms4090\">https://doi.org/10.1038/ncomms4090</a>.","apa":"Le, J., Liu, X., Yang, K., Chen, X., Zhu, L., Wang, H., … Sack, F. (2014). Auxin transport and activity regulate stomatal patterning and development. <i>Nature Communications</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncomms4090\">https://doi.org/10.1038/ncomms4090</a>","short":"J. Le, X. Liu, K. Yang, X. Chen, L. Zhu, H. Wang, M. Wang, S. Vanneste, M. Morita, M. Tasaka, Z. Ding, J. Friml, T. Beeckman, F. Sack, Nature Communications 5 (2014)."},"publication_status":"published"},{"project":[{"grant_number":"207362","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Hormonal cross-talk in plant organogenesis"}],"publication_status":"published","citation":{"short":"P. Marhavý, J. Duclercq, B. Weller, E. Feraru, A. Bielach, R. Offringa, J. Friml, C. Schwechheimer, A. Murphy, E. Benková, Current Biology 24 (2014) 1031–1037.","apa":"Marhavý, P., Duclercq, J., Weller, B., Feraru, E., Bielach, A., Offringa, R., … Benková, E. (2014). Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2014.04.002\">https://doi.org/10.1016/j.cub.2014.04.002</a>","ista":"Marhavý P, Duclercq J, Weller B, Feraru E, Bielach A, Offringa R, Friml J, Schwechheimer C, Murphy A, Benková E. 2014. Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis. Current Biology. 24(9), 1031–1037.","chicago":"Marhavý, Peter, Jérôme Duclercq, Benjamin Weller, Elena Feraru, Agnieszka Bielach, Remko Offringa, Jiří Friml, Claus Schwechheimer, Angus Murphy, and Eva Benková. “Cytokinin Controls Polarity of PIN1-Dependent Auxin Transport during Lateral Root Organogenesis.” <i>Current Biology</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cub.2014.04.002\">https://doi.org/10.1016/j.cub.2014.04.002</a>.","ieee":"P. Marhavý <i>et al.</i>, “Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis,” <i>Current Biology</i>, vol. 24, no. 9. Cell Press, pp. 1031–1037, 2014.","mla":"Marhavý, Peter, et al. “Cytokinin Controls Polarity of PIN1-Dependent Auxin Transport during Lateral Root Organogenesis.” <i>Current Biology</i>, vol. 24, no. 9, Cell Press, 2014, pp. 1031–37, doi:<a href=\"https://doi.org/10.1016/j.cub.2014.04.002\">10.1016/j.cub.2014.04.002</a>.","ama":"Marhavý P, Duclercq J, Weller B, et al. Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis. <i>Current Biology</i>. 2014;24(9):1031-1037. doi:<a href=\"https://doi.org/10.1016/j.cub.2014.04.002\">10.1016/j.cub.2014.04.002</a>"},"page":"1031 - 1037","date_created":"2018-12-11T11:54:48Z","type":"journal_article","author":[{"last_name":"Marhavy","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5227-5741","full_name":"Marhavy, Peter","first_name":"Peter"},{"first_name":"Jérôme","full_name":"Duclercq, Jérôme","last_name":"Duclercq"},{"last_name":"Weller","first_name":"Benjamin","full_name":"Weller, Benjamin"},{"last_name":"Feraru","first_name":"Elena","full_name":"Feraru, Elena"},{"first_name":"Agnieszka","full_name":"Bielach, Agnieszka","last_name":"Bielach"},{"last_name":"Offringa","full_name":"Offringa, Remko","first_name":"Remko"},{"first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"full_name":"Schwechheimer, Claus","first_name":"Claus","last_name":"Schwechheimer"},{"last_name":"Murphy","full_name":"Murphy, Angus","first_name":"Angus"},{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","first_name":"Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"issue":"9","intvolume":"        24","abstract":[{"lang":"eng","text":"The plant hormones auxin and cytokinin mutually coordinate their activities to control various aspects of development [1-9], and their crosstalk occurs at multiple levels [10, 11]. Cytokinin-mediated modulation of auxin transport provides an efficient means to regulate auxin distribution in plant organs. Here, we demonstrate that cytokinin does not merely control the overall auxin flow capacity, but might also act as a polarizing cue and control the auxin stream directionality during plant organogenesis. Cytokinin enhances the PIN-FORMED1 (PIN1) auxin transporter depletion at specific polar domains, thus rearranging the cellular PIN polarities and directly regulating the auxin flow direction. This selective cytokinin sensitivity correlates with the PIN protein phosphorylation degree. PIN1 phosphomimicking mutations, as well as enhanced phosphorylation in plants with modulated activities of PIN-specific kinases and phosphatases, desensitize PIN1 to cytokinin. Our results reveal conceptually novel, cytokinin-driven polarization mechanism that operates in developmental processes involving rapid auxin stream redirection, such as lateral root organogenesis, in which a gradual PIN polarity switch defines the growth axis of the newly formed organ."}],"department":[{"_id":"EvBe"},{"_id":"JiFr"}],"date_published":"2014-05-05T00:00:00Z","external_id":{"isi":["000335542300029"]},"language":[{"iso":"eng"}],"ec_funded":1,"article_processing_charge":"No","scopus_import":"1","publication":"Current Biology","status":"public","publist_id":"5160","oa_version":"None","isi":1,"quality_controlled":"1","fulldoi":"https://doi.org/10.1016/j.cub.2014.04.002","volume":24,"date_updated":"2025-09-29T12:09:47Z","month":"05","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.1016/j.cub.2014.04.002","publisher":"Cell Press","_id":"1934","day":"05","year":"2014","corr_author":"1","title":"Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis"},{"_id":"1994","publisher":"Cell Press","doi":"10.1016/j.cub.2014.09.056","title":"Directional auxin transport mechanisms in early diverging land plants","corr_author":"1","year":"2014","day":"01","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"12","fulldoi":"https://doi.org/10.1016/j.cub.2014.09.056","quality_controlled":"1","date_updated":"2025-09-29T12:07:20Z","volume":24,"oa_version":"None","isi":1,"scopus_import":"1","status":"public","publication":"Current Biology","publist_id":"5088","article_processing_charge":"No","ec_funded":1,"external_id":{"isi":["000345808700019"]},"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"date_published":"2014-12-01T00:00:00Z","abstract":[{"lang":"eng","text":"The emergence and radiation of multicellular land plants was driven by crucial innovations to their body plans [1]. The directional transport of the phytohormone auxin represents a key, plant-specific mechanism for polarization and patterning in complex seed plants [2-5]. Here, we show that already in the early diverging land plant lineage, as exemplified by the moss Physcomitrella patens, auxin transport by PIN transporters is operational and diversified into ER-localized and plasma membrane-localized PIN proteins. Gain-of-function and loss-of-function analyses revealed that PIN-dependent intercellular auxin transport in Physcomitrella mediates crucial developmental transitions in tip-growing filaments and waves of polarization and differentiation in leaf-like structures. Plasma membrane PIN proteins localize in a polar manner to the tips of moss filaments, revealing an unexpected relation between polarization mechanisms in moss tip-growing cells and multicellular tissues of seed plants. Our results trace the origins of polarization and auxin-mediated patterning mechanisms and highlight the crucial role of polarized auxin transport during the evolution of multicellular land plants."}],"intvolume":"        24","author":[{"first_name":"Tom","full_name":"Viaene, Tom","last_name":"Viaene"},{"last_name":"Landberg","first_name":"Katarina","full_name":"Landberg, Katarina"},{"last_name":"Thelander","first_name":"Mattias","full_name":"Thelander, Mattias"},{"first_name":"Eva","full_name":"Medvecka, Eva","last_name":"Medvecka"},{"last_name":"Pederson","first_name":"Eric","full_name":"Pederson, Eric"},{"full_name":"Feraru, Elena","first_name":"Elena","last_name":"Feraru"},{"last_name":"Cooper","full_name":"Cooper, Endymion","first_name":"Endymion"},{"first_name":"Mansour","full_name":"Karimi, Mansour","last_name":"Karimi"},{"first_name":"Charles","full_name":"Delwiche, Charles","last_name":"Delwiche"},{"last_name":"Ljung","full_name":"Ljung, Karin","first_name":"Karin"},{"last_name":"Geisler","full_name":"Geisler, Markus","first_name":"Markus"},{"last_name":"Sundberg","full_name":"Sundberg, Eva","first_name":"Eva"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","first_name":"Jirí"}],"issue":"23","type":"journal_article","date_created":"2018-12-11T11:55:06Z","page":"2786 - 2791","publication_status":"published","project":[{"name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300"}],"citation":{"short":"T. Viaene, K. Landberg, M. Thelander, E. Medvecka, E. Pederson, E. Feraru, E. Cooper, M. Karimi, C. Delwiche, K. Ljung, M. Geisler, E. Sundberg, J. Friml, Current Biology 24 (2014) 2786–2791.","apa":"Viaene, T., Landberg, K., Thelander, M., Medvecka, E., Pederson, E., Feraru, E., … Friml, J. (2014). Directional auxin transport mechanisms in early diverging land plants. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2014.09.056\">https://doi.org/10.1016/j.cub.2014.09.056</a>","ama":"Viaene T, Landberg K, Thelander M, et al. Directional auxin transport mechanisms in early diverging land plants. <i>Current Biology</i>. 2014;24(23):2786-2791. doi:<a href=\"https://doi.org/10.1016/j.cub.2014.09.056\">10.1016/j.cub.2014.09.056</a>","chicago":"Viaene, Tom, Katarina Landberg, Mattias Thelander, Eva Medvecka, Eric Pederson, Elena Feraru, Endymion Cooper, et al. “Directional Auxin Transport Mechanisms in Early Diverging Land Plants.” <i>Current Biology</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cub.2014.09.056\">https://doi.org/10.1016/j.cub.2014.09.056</a>.","ieee":"T. Viaene <i>et al.</i>, “Directional auxin transport mechanisms in early diverging land plants,” <i>Current Biology</i>, vol. 24, no. 23. Cell Press, pp. 2786–2791, 2014.","mla":"Viaene, Tom, et al. “Directional Auxin Transport Mechanisms in Early Diverging Land Plants.” <i>Current Biology</i>, vol. 24, no. 23, Cell Press, 2014, pp. 2786–91, doi:<a href=\"https://doi.org/10.1016/j.cub.2014.09.056\">10.1016/j.cub.2014.09.056</a>.","ista":"Viaene T, Landberg K, Thelander M, Medvecka E, Pederson E, Feraru E, Cooper E, Karimi M, Delwiche C, Ljung K, Geisler M, Sundberg E, Friml J. 2014. Directional auxin transport mechanisms in early diverging land plants. Current Biology. 24(23), 2786–2791."}},{"date_created":"2018-12-11T11:55:07Z","page":"E5471 - E5479","issue":"50","author":[{"full_name":"Hazak, Ora","first_name":"Ora","last_name":"Hazak"},{"last_name":"Obolski","first_name":"Uri","full_name":"Obolski, Uri"},{"first_name":"Tomas","full_name":"Prat, Tomas","id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87","last_name":"Prat"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří"},{"first_name":"Lilach","full_name":"Hadany, Lilach","last_name":"Hadany"},{"full_name":"Yalovsky, Shaul","first_name":"Shaul","last_name":"Yalovsky"}],"type":"journal_article","publication_status":"published","citation":{"ama":"Hazak O, Obolski U, Prat T, Friml J, Hadany L, Yalovsky S. Bimodal regulation of ICR1 levels generates self-organizing auxin distribution. <i>PNAS</i>. 2014;111(50):E5471-E5479. doi:<a href=\"https://doi.org/10.1073/pnas.1413918111\">10.1073/pnas.1413918111</a>","ieee":"O. Hazak, U. Obolski, T. Prat, J. Friml, L. Hadany, and S. Yalovsky, “Bimodal regulation of ICR1 levels generates self-organizing auxin distribution,” <i>PNAS</i>, vol. 111, no. 50. National Academy of Sciences, pp. E5471–E5479, 2014.","chicago":"Hazak, Ora, Uri Obolski, Tomas Prat, Jiří Friml, Lilach Hadany, and Shaul Yalovsky. “Bimodal Regulation of ICR1 Levels Generates Self-Organizing Auxin Distribution.” <i>PNAS</i>. National Academy of Sciences, 2014. <a href=\"https://doi.org/10.1073/pnas.1413918111\">https://doi.org/10.1073/pnas.1413918111</a>.","mla":"Hazak, Ora, et al. “Bimodal Regulation of ICR1 Levels Generates Self-Organizing Auxin Distribution.” <i>PNAS</i>, vol. 111, no. 50, National Academy of Sciences, 2014, pp. E5471–79, doi:<a href=\"https://doi.org/10.1073/pnas.1413918111\">10.1073/pnas.1413918111</a>.","ista":"Hazak O, Obolski U, Prat T, Friml J, Hadany L, Yalovsky S. 2014. Bimodal regulation of ICR1 levels generates self-organizing auxin distribution. PNAS. 111(50), E5471–E5479.","short":"O. Hazak, U. Obolski, T. Prat, J. Friml, L. Hadany, S. Yalovsky, PNAS 111 (2014) E5471–E5479.","apa":"Hazak, O., Obolski, U., Prat, T., Friml, J., Hadany, L., &#38; Yalovsky, S. (2014). Bimodal regulation of ICR1 levels generates self-organizing auxin distribution. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1413918111\">https://doi.org/10.1073/pnas.1413918111</a>"},"scopus_import":"1","publist_id":"5083","publication":"PNAS","status":"public","date_published":"2014-12-16T00:00:00Z","department":[{"_id":"JiFr"}],"intvolume":"       111","abstract":[{"lang":"eng","text":"Auxin polar transport, local maxima, and gradients have become an importantmodel system for studying self-organization. Auxin distribution is regulated by auxin-dependent positive feedback loops that are not well-understood at the molecular level. Previously, we showed the involvement of the RHO of Plants (ROP) effector INTERACTOR of CONSTITUTIVELY active ROP 1 (ICR1) in regulation of auxin transport and that ICR1 levels are posttranscriptionally repressed at the site of maximum auxin accumulation at the root tip. Here, we show that bimodal regulation of ICR1 levels by auxin is essential for regulating formation of auxin local maxima and gradients. ICR1 levels increase concomitant with increase in auxin response in lateral root primordia, cotyledon tips, and provascular tissues. However, in the embryo hypophysis and root meristem, when auxin exceeds critical levels, ICR1 is rapidly destabilized by an SCF(TIR1/AFB) [SKP, Cullin, F-box (transport inhibitor response 1/auxin signaling F-box protein)]-dependent auxin signaling mechanism. Furthermore, ectopic expression of ICR1 in the embryo hypophysis resulted in reduction of auxin accumulation and concomitant root growth arrest. ICR1 disappeared during root regeneration and lateral root initiation concomitantly with the formation of a local auxin maximum in response to external auxin treatments and transiently after gravitropic stimulation. Destabilization of ICR1 was impaired after inhibition of auxin transport and signaling, proteasome function, and protein synthesis. A mathematical model based on these findings shows that an in vivo-like auxin distribution, rootward auxin flux, and shootward reflux can be simulated without assuming preexisting tissue polarity. Our experimental results and mathematical modeling indicate that regulation of auxin distribution is tightly associated with auxin-dependent ICR1 levels."}],"article_processing_charge":"No","external_id":{"isi":["000346366500020"]},"oa":1,"language":[{"iso":"eng"}],"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273421/","open_access":"1"}],"quality_controlled":"1","fulldoi":"https://doi.org/10.1073/pnas.1413918111","volume":111,"date_updated":"2025-09-29T12:06:13Z","oa_version":"Submitted Version","isi":1,"publisher":"National Academy of Sciences","_id":"1996","doi":"10.1073/pnas.1413918111","year":"2014","day":"16","title":"Bimodal regulation of ICR1 levels generates self-organizing auxin distribution","month":"12","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"publication_status":"published","citation":{"apa":"Mazur, E., Kurczyñska, E., &#38; Friml, J. (2014). Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis. <i>Protoplasma</i>. Springer. <a href=\"https://doi.org/10.1007/s00709-014-0620-5\">https://doi.org/10.1007/s00709-014-0620-5</a>","short":"E. Mazur, E. Kurczyñska, J. Friml, Protoplasma 251 (2014) 1125–1139.","ista":"Mazur E, Kurczyñska E, Friml J. 2014. Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis. Protoplasma. 251(5), 1125–1139.","mla":"Mazur, Ewa, et al. “Cellular Events during Interfascicular Cambium Ontogenesis in Inflorescence Stems of Arabidopsis.” <i>Protoplasma</i>, vol. 251, no. 5, Springer, 2014, pp. 1125–39, doi:<a href=\"https://doi.org/10.1007/s00709-014-0620-5\">10.1007/s00709-014-0620-5</a>.","ieee":"E. Mazur, E. Kurczyñska, and J. Friml, “Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis,” <i>Protoplasma</i>, vol. 251, no. 5. Springer, pp. 1125–1139, 2014.","ama":"Mazur E, Kurczyñska E, Friml J. Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis. <i>Protoplasma</i>. 2014;251(5):1125-1139. doi:<a href=\"https://doi.org/10.1007/s00709-014-0620-5\">10.1007/s00709-014-0620-5</a>","chicago":"Mazur, Ewa, Ewa Kurczyñska, and Jiří Friml. “Cellular Events during Interfascicular Cambium Ontogenesis in Inflorescence Stems of Arabidopsis.” <i>Protoplasma</i>. Springer, 2014. <a href=\"https://doi.org/10.1007/s00709-014-0620-5\">https://doi.org/10.1007/s00709-014-0620-5</a>."},"author":[{"last_name":"Mazur","first_name":"Ewa","full_name":"Mazur, Ewa"},{"full_name":"Kurczyñska, Ewa","first_name":"Ewa","last_name":"Kurczyñska"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"}],"issue":"5","type":"journal_article","date_created":"2018-12-11T11:55:29Z","page":"1125 - 1139","article_processing_charge":"No","external_id":{"isi":["000340480300012"]},"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"date_published":"2014-02-14T00:00:00Z","abstract":[{"lang":"eng","text":"Development of cambium and its activity is important for our knowledge of the mechanism of secondary growth. Arabidopsis thaliana emerges as a good model plant for such a kind of study. Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC). These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions. Distribution of auxin was observed to be higher in differentiating into cambium parenchyma cells compared to cells within the pith and cortex. Expression of PIN1 in IPC was always preceded by auxin accumulation. Basal localization of PIN1 was already established in the cells prior to their periclinal division. These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles. The final consequence of which was the closure of the cambial ring within the stem. Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs). In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis."}],"intvolume":"       251","scopus_import":"1","publication":"Protoplasma","status":"public","publist_id":"4985","oa_version":"None","isi":1,"fulldoi":"https://doi.org/10.1007/s00709-014-0620-5","quality_controlled":"1","date_updated":"2025-09-29T11:48:32Z","volume":251,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"02","publisher":"Springer","_id":"2061","doi":"10.1007/s00709-014-0620-5","title":"Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis","year":"2014","day":"14"},{"language":[{"iso":"eng"}],"date_published":"2014-04-01T00:00:00Z","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","month":"04","department":[{"_id":"JiFr"}],"abstract":[{"lang":"eng","text":"The generation of asymmetry, at both cellular and tissue level, is one of the most essential capabilities of all eukaryotic organisms. It mediates basically all multicellular development ranging from embryogenesis and de novo organ formation till responses to various environmental stimuli. In plants, the awe-inspiring number of such processes is regulated by phytohormone auxin and its directional, cell-to-cell transport. The mediators of this transport, PIN auxin transporters, are asymmetrically localized at the plasma membrane, and this polar localization determines the directionality of intercellular auxin flow. Thus, auxin transport contributes crucially to the generation of local auxin gradients or maxima, which instruct given cell to change its developmental program. Here, we introduce and discuss the molecular components and cellular mechanisms regulating the generation and maintenance of cellular PIN polarity, as the general hallmarks of cell polarity in plants."}],"scopus_import":1,"_id":"1806","publisher":"Springer","doi":"10.1007/978-3-7091-1526-8_8","title":"Auxin on the road navigated by cellular PIN polarity","publication":"Auxin and Its Role in Plant Development","status":"public","publist_id":"5304","corr_author":"1","year":"2014","day":"01","publication_status":"published","oa_version":"None","editor":[{"last_name":"Zažímalová","full_name":"Zažímalová, Eva","first_name":"Eva"},{"last_name":"Petrášek","first_name":"Jan","full_name":"Petrášek, Jan"},{"first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"}],"citation":{"mla":"Baster, Pawel, and Jiří Friml. “Auxin on the Road Navigated by Cellular PIN Polarity.” <i>Auxin and Its Role in Plant Development</i>, edited by Eva Zažímalová et al., Springer, 2014, pp. 143–70, doi:<a href=\"https://doi.org/10.1007/978-3-7091-1526-8_8\">10.1007/978-3-7091-1526-8_8</a>.","chicago":"Baster, Pawel, and Jiří Friml. “Auxin on the Road Navigated by Cellular PIN Polarity.” In <i>Auxin and Its Role in Plant Development</i>, edited by Eva Zažímalová, Jan Petrášek, and Eva Benková, 143–70. Springer, 2014. <a href=\"https://doi.org/10.1007/978-3-7091-1526-8_8\">https://doi.org/10.1007/978-3-7091-1526-8_8</a>.","ama":"Baster P, Friml J. Auxin on the road navigated by cellular PIN polarity. In: Zažímalová E, Petrášek J, Benková E, eds. <i>Auxin and Its Role in Plant Development</i>. Springer; 2014:143-170. doi:<a href=\"https://doi.org/10.1007/978-3-7091-1526-8_8\">10.1007/978-3-7091-1526-8_8</a>","ieee":"P. Baster and J. Friml, “Auxin on the road navigated by cellular PIN polarity,” in <i>Auxin and Its Role in Plant Development</i>, E. Zažímalová, J. Petrášek, and E. Benková, Eds. Springer, 2014, pp. 143–170.","ista":"Baster P, Friml J. 2014.Auxin on the road navigated by cellular PIN polarity. In: Auxin and Its Role in Plant Development. , 143–170.","apa":"Baster, P., &#38; Friml, J. (2014). Auxin on the road navigated by cellular PIN polarity. In E. Zažímalová, J. Petrášek, &#38; E. Benková (Eds.), <i>Auxin and Its Role in Plant Development</i> (pp. 143–170). Springer. <a href=\"https://doi.org/10.1007/978-3-7091-1526-8_8\">https://doi.org/10.1007/978-3-7091-1526-8_8</a>","short":"P. Baster, J. Friml, in:, E. Zažímalová, J. Petrášek, E. Benková (Eds.), Auxin and Its Role in Plant Development, Springer, 2014, pp. 143–170."},"fulldoi":"https://doi.org/10.1007/978-3-7091-1526-8_8","quality_controlled":"1","author":[{"full_name":"Baster, Pawel","first_name":"Pawel","last_name":"Baster","id":"3028BD74-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"type":"book_chapter","date_updated":"2024-10-09T20:55:54Z","date_created":"2018-12-11T11:54:07Z","page":"143 - 170"},{"OA_place":"publisher","article_processing_charge":"No","language":[{"iso":"eng"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","date_published":"2014-12-01T00:00:00Z","supervisor":[{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"month":"12","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"abstract":[{"lang":"eng","text":"Phosphatidylinositol (Ptdlns) is a structural phospholipid that can be phosphorylated into various lipid signaling molecules, designated polyphosphoinositides (PPIs). The reversible phosphorylation of PPIs on the 3, 4, or 5 position of inositol is performed by a set of organelle-specific kinases and phosphatases, and the characteristic head groups make these molecules ideal for regulating biological processes in time and space. In yeast and mammals, Ptdlns3P and Ptdlns(3,5)P2 play crucial roles in trafficking toward the lytic compartments, whereas the role in plants is not yet fully understood. Here we identified the role of a land plant-specific subgroup of PPI phosphatases, the suppressor of actin 2 (SAC2) to SAC5, during vauolar trafficking and morphogenesis in Arabidopsis thaliana. SAC2-SAC5 localize to the tonoplast along with Ptdlns3P, the presumable product of their activity. in SAC gain- and loss-of-function mutants, the levels of Ptdlns monophosphates and bisphosphates were changed, with opposite effects on the morphology of storage and lytic vacuoles, and the trafficking toward the vacuoles was defective. Moreover, multiple sac knockout mutants had an increased number of smaller storage and lytic vacuoles, whereas extralarge vacuoles were observed in the overexpression lines, correlating with various growth and developmental defects. The fragmented vacuolar phenotype of sac mutants could be mimicked by treating wild-type seedlings with Ptdlns(3,5)P2, corroborating that this PPI is important for vacuole morphology. Taken together, these results provide evidence that PPIs, together with their metabolic enzymes SAC2-SAC5, are crucial for vacuolar trafficking and for vacuolar morphology and function in plants."}],"title":"Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana","corr_author":"1","publist_id":"5805","status":"public","year":"2014","day":"01","degree_awarded":"PhD","_id":"1402","publisher":"Institute of Science and Technology Austria","citation":{"ista":"Marhavá P. 2014. Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana. Institute of Science and Technology Austria.","ama":"Marhavá P. Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana. 2014.","chicago":"Marhavá, Petra. “Molecular Mechanisms of Patterning and Subcellular Trafficking in Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2014.","mla":"Marhavá, Petra. <i>Molecular Mechanisms of Patterning and Subcellular Trafficking in Arabidopsis Thaliana</i>. Institute of Science and Technology Austria, 2014.","ieee":"P. Marhavá, “Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana,” Institute of Science and Technology Austria, 2014.","apa":"Marhavá, P. (2014). <i>Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana</i>. Institute of Science and Technology Austria.","short":"P. Marhavá, Molecular Mechanisms of Patterning and Subcellular Trafficking in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2014."},"doi_confirm":"1","publication_status":"published","oa_version":"None","author":[{"last_name":"Marhavá","id":"44E59624-F248-11E8-B48F-1D18A9856A87","full_name":"Marhavá, Petra","first_name":"Petra"}],"date_updated":"2026-07-29T10:06:44Z","type":"dissertation","alternative_title":["ISTA Thesis"],"date_created":"2018-12-11T11:51:49Z","page":"90","publication_identifier":{"issn":["2663-337X"]}},{"publication_status":"published","license":"https://creativecommons.org/licenses/by/3.0/","file":[{"content_type":"application/pdf","file_name":"2013_Plants_Vanneste.pdf","success":1,"access_level":"open_access","file_size":670188,"creator":"dernst","date_updated":"2022-03-21T12:12:56Z","date_created":"2022-03-21T12:12:56Z","relation":"main_file","checksum":"fb4ff2e820e344e253c9197544610be6","file_id":"10916"}],"citation":{"ama":"Vanneste S, Friml J. Calcium: The missing link in auxin action. <i>Plants</i>. 2013;2(4):650-675. doi:<a href=\"https://doi.org/10.3390/plants2040650\">10.3390/plants2040650</a>","chicago":"Vanneste, Steffen, and Jiří Friml. “Calcium: The Missing Link in Auxin Action.” <i>Plants</i>. MDPI, 2013. <a href=\"https://doi.org/10.3390/plants2040650\">https://doi.org/10.3390/plants2040650</a>.","ieee":"S. Vanneste and J. Friml, “Calcium: The missing link in auxin action,” <i>Plants</i>, vol. 2, no. 4. MDPI, pp. 650–675, 2013.","mla":"Vanneste, Steffen, and Jiří Friml. “Calcium: The Missing Link in Auxin Action.” <i>Plants</i>, vol. 2, no. 4, MDPI, 2013, pp. 650–75, doi:<a href=\"https://doi.org/10.3390/plants2040650\">10.3390/plants2040650</a>.","ista":"Vanneste S, Friml J. 2013. Calcium: The missing link in auxin action. Plants. 2(4), 650–675.","apa":"Vanneste, S., &#38; Friml, J. (2013). Calcium: The missing link in auxin action. <i>Plants</i>. MDPI. <a href=\"https://doi.org/10.3390/plants2040650\">https://doi.org/10.3390/plants2040650</a>","short":"S. Vanneste, J. Friml, Plants 2 (2013) 650–675."},"file_date_updated":"2022-03-21T12:12:56Z","author":[{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"}],"issue":"4","type":"journal_article","keyword":["Plant Science","Ecology","Ecology","Evolution","Behavior and Systematics"],"date_created":"2022-03-21T07:13:49Z","page":"650-675","article_processing_charge":"No","external_id":{"pmid":["27137397"]},"oa":1,"language":[{"iso":"eng"}],"date_published":"2013-10-21T00:00:00Z","department":[{"_id":"JiFr"}],"intvolume":"         2","abstract":[{"text":"Due to their sessile lifestyles, plants need to deal with the limitations and stresses imposed by the changing environment. Plants cope with these by a remarkable developmental flexibility, which is embedded in their strategy to survive. Plants can adjust their size, shape and number of organs, bend according to gravity and light, and regenerate tissues that were damaged, utilizing a coordinating, intercellular signal, the plant hormone, auxin. Another versatile signal is the cation, Ca2+, which is a crucial second messenger for many rapid cellular processes during responses to a wide range of endogenous and environmental signals, such as hormones, light, drought stress and others. Auxin is a good candidate for one of these Ca2+-activating signals. However, the role of auxin-induced Ca2+ signaling is poorly understood. Here, we will provide an overview of possible developmental and physiological roles, as well as mechanisms underlying the interconnection of Ca2+ and auxin signaling. ","lang":"eng"}],"pmid":1,"has_accepted_license":"1","scopus_import":"1","ddc":["580"],"publication":"Plants","status":"public","oa_version":"Published Version","fulldoi":"https://doi.org/10.3390/plants2040650","quality_controlled":"1","publication_identifier":{"issn":["2223-7747"]},"date_updated":"2024-10-09T21:01:52Z","volume":2,"article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"10","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/3.0/legalcode","name":"Creative Commons Attribution 3.0 Unported (CC BY 3.0)","short":"CC BY (3.0)","image":"/images/cc_by.png"},"publisher":"MDPI","_id":"10895","doi":"10.3390/plants2040650","title":"Calcium: The missing link in auxin action","corr_author":"1","year":"2013","day":"21"}]