[{"page":"329-343","article_processing_charge":"No","date_published":"2022-01-01T00:00:00Z","pmid":1,"year":"2022","publisher":"Wiley","isi":1,"author":[{"last_name":"Kashkan","full_name":"Kashkan, Ivan","first_name":"Ivan"},{"full_name":"Hrtyan, Mónika","id":"45A71A74-F248-11E8-B48F-1D18A9856A87","last_name":"Hrtyan","first_name":"Mónika"},{"full_name":"Retzer, Katarzyna","last_name":"Retzer","first_name":"Katarzyna"},{"full_name":"Humpolíčková, Jana","last_name":"Humpolíčková","first_name":"Jana"},{"first_name":"Aswathy","full_name":"Jayasree, Aswathy","last_name":"Jayasree"},{"full_name":"Filepová, Roberta","last_name":"Filepová","first_name":"Roberta"},{"first_name":"Zuzana","last_name":"Vondráková","full_name":"Vondráková, Zuzana"},{"last_name":"Simon","full_name":"Simon, Sibu","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741","first_name":"Sibu"},{"full_name":"Rombaut, Debbie","last_name":"Rombaut","first_name":"Debbie"},{"first_name":"Thomas B.","full_name":"Jacobs, Thomas B.","last_name":"Jacobs"},{"full_name":"Frilander, Mikko J.","last_name":"Frilander","first_name":"Mikko J."},{"first_name":"Jan","full_name":"Hejátko, Jan","last_name":"Hejátko"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"Jan","last_name":"Petrášek","full_name":"Petrášek, Jan"},{"first_name":"Kamil","full_name":"Růžička, Kamil","last_name":"Růžička"}],"_id":"10282","external_id":{"isi":["000714678100001"],"pmid":["34637542"]},"issue":"1","volume":233,"intvolume":"       233","quality_controlled":"1","oa_version":"Preprint","language":[{"iso":"eng"}],"publication_status":"published","department":[{"_id":"JiFr"}],"day":"01","doi":"10.1111/nph.17792","month":"01","acknowledgement":"We thank Claus Schwechheimer for the pin34 and pin347 seeds, Yuliia Mironova for technical assistance, Ksenia Timofeyenko and Dmitry Konovalov for help with the evolutional analysis, Konstantin Kutashev and Siarhei Dabravolski for assistance with FRET-FLIM, Huibin Han for advice with hypocotyl imaging, Karel Müller for the initial qRT-PCR on the tobacco cell lines, Stano Pekár for suggestions regarding the statistical analysis of the morphodynamic measurements, and Jozef Mravec, Dolf Weijers and Lindy Abas for their comments on the manuscript. This work was supported by the Czech Science Foundation (projects 16-26428S and 19-23773S to IK, MH and KRůžička, 19-18917S to JHumpolíčková and 18-26981S to JF), and the Ministry of Education, Youth and Sports of the Czech Republic (MEYS, CZ.02.1.01/0.0/0.0/16_019/0000738) to KRůžička and JHejátko. The imaging facilities of the Institute of Experimental Botany and CEITEC are supported by MEYS (LM2018129 – Czech BioImaging and CZ.02.1.01/0.0/0.0/16_013/0001775). The authors declare no competing interests.","scopus_import":"1","citation":{"apa":"Kashkan, I., Hrtyan, M., Retzer, K., Humpolíčková, J., Jayasree, A., Filepová, R., … Růžička, K. (2022). Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.17792\">https://doi.org/10.1111/nph.17792</a>","ama":"Kashkan I, Hrtyan M, Retzer K, et al. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. <i>New Phytologist</i>. 2022;233(1):329-343. doi:<a href=\"https://doi.org/10.1111/nph.17792\">10.1111/nph.17792</a>","ieee":"I. Kashkan <i>et al.</i>, “Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 233, no. 1. Wiley, pp. 329–343, 2022.","ista":"Kashkan I, Hrtyan M, Retzer K, Humpolíčková J, Jayasree A, Filepová R, Vondráková Z, Simon S, Rombaut D, Jacobs TB, Frilander MJ, Hejátko J, Friml J, Petrášek J, Růžička K. 2022. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist. 233(1), 329–343.","mla":"Kashkan, Ivan, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 233, no. 1, Wiley, 2022, pp. 329–43, doi:<a href=\"https://doi.org/10.1111/nph.17792\">10.1111/nph.17792</a>.","chicago":"Kashkan, Ivan, Mónika Hrtyan, Katarzyna Retzer, Jana Humpolíčková, Aswathy Jayasree, Roberta Filepová, Zuzana Vondráková, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2022. <a href=\"https://doi.org/10.1111/nph.17792\">https://doi.org/10.1111/nph.17792</a>.","short":"I. Kashkan, M. Hrtyan, K. Retzer, J. Humpolíčková, A. Jayasree, R. Filepová, Z. Vondráková, S. Simon, D. Rombaut, T.B. Jacobs, M.J. Frilander, J. Hejátko, J. Friml, J. Petrášek, K. Růžička, New Phytologist 233 (2022) 329–343."},"status":"public","main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/2020.05.02.074070v2"}],"oa":1,"title":"Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","article_type":"original","type":"journal_article","date_updated":"2024-05-22T11:33:15Z","date_created":"2021-11-14T23:01:24Z","publication":"New Phytologist","publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"abstract":[{"text":"Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development.","lang":"eng"}]},{"oa":1,"has_accepted_license":"1","article_type":"original","title":"Bending to auxin: Fast acid growth for tropisms","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2023-11-02T17:00:03Z","type":"journal_article","date_updated":"2026-04-07T14:18:57Z","abstract":[{"text":"The phytohormone auxin is the major growth regulator governing tropic responses including gravitropism. Auxin build-up at the lower side of stimulated shoots promotes cell expansion, whereas in roots it inhibits growth, leading to upward shoot bending and downward root bending, respectively. Yet it remains an enigma how the same signal can trigger such opposite cellular responses. In this review, we discuss several recent unexpected insights into the mechanisms underlying auxin regulation of growth, challenging several existing models. We focus on the divergent mechanisms of apoplastic pH regulation in shoots and roots revisiting the classical Acid Growth Theory and discuss coordinated involvement of multiple auxin signaling pathways. From this emerges a more comprehensive, updated picture how auxin regulates growth.","lang":"eng"}],"date_created":"2021-12-05T23:01:43Z","publication":"Trends in Plant Science","publication_identifier":{"issn":["1360-1385"]},"oa_version":"Submitted Version","quality_controlled":"1","language":[{"iso":"eng"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"11626"}]},"month":"05","corr_author":"1","publication_status":"published","department":[{"_id":"JiFr"}],"day":"01","doi":"10.1016/j.tplants.2021.11.006","acknowledgement":"The authors thank Alexandra Mally for editing the text. This work was supported by the Austrian Science Fund (FWF) I 3630-B25 to Jiří Friml and the DOC Fellowship of the Austrian Academy of Sciences to Lanxin Li. All figures were created with BioRender.com.","scopus_import":"1","status":"public","citation":{"ieee":"L. Li, M. C. Gallei, and J. Friml, “Bending to auxin: Fast acid growth for tropisms,” <i>Trends in Plant Science</i>, vol. 27, no. 5. Cell Press, pp. 440–449, 2022.","apa":"Li, L., Gallei, M. C., &#38; Friml, J. (2022). Bending to auxin: Fast acid growth for tropisms. <i>Trends in Plant Science</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">https://doi.org/10.1016/j.tplants.2021.11.006</a>","ama":"Li L, Gallei MC, Friml J. Bending to auxin: Fast acid growth for tropisms. <i>Trends in Plant Science</i>. 2022;27(5):440-449. doi:<a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">10.1016/j.tplants.2021.11.006</a>","chicago":"Li, Lanxin, Michelle C Gallei, and Jiří Friml. “Bending to Auxin: Fast Acid Growth for Tropisms.” <i>Trends in Plant Science</i>. Cell Press, 2022. <a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">https://doi.org/10.1016/j.tplants.2021.11.006</a>.","short":"L. Li, M.C. Gallei, J. Friml, Trends in Plant Science 27 (2022) 440–449.","mla":"Li, Lanxin, et al. “Bending to Auxin: Fast Acid Growth for Tropisms.” <i>Trends in Plant Science</i>, vol. 27, no. 5, Cell Press, 2022, pp. 440–49, doi:<a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">10.1016/j.tplants.2021.11.006</a>.","ista":"Li L, Gallei MC, Friml J. 2022. Bending to auxin: Fast acid growth for tropisms. Trends in Plant Science. 27(5), 440–449."},"file":[{"checksum":"3d94980ee1ff6bec100dd813f6a921a6","relation":"main_file","content_type":"application/pdf","access_level":"open_access","success":1,"creator":"amally","file_size":805779,"date_updated":"2023-11-02T17:00:03Z","file_name":"Li Plants 2021_accepted.pdf","file_id":"14480","date_created":"2023-11-02T17:00:03Z"}],"author":[{"last_name":"Li","full_name":"Li, Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5607-272X","first_name":"Lanxin"},{"first_name":"Michelle C","orcid":"0000-0003-1286-7368","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml"}],"isi":1,"_id":"10411","issue":"5","external_id":{"pmid":["34848141"],"isi":["000793707900005"]},"volume":27,"intvolume":"        27","article_processing_charge":"No","page":"440-449","pmid":1,"date_published":"2022-05-01T00:00:00Z","year":"2022","project":[{"grant_number":"I03630","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425"},{"_id":"26B4D67E-B435-11E9-9278-68D0E5697425","name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root","grant_number":"25351"}],"publisher":"Cell Press","ddc":["580"]},{"keyword":["flavonols","MAX2","rac-Gr24","RNA-seq","root development","transcriptional regulation"],"oa":1,"article_type":"original","title":"Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-06-18T08:43:19Z","type":"journal_article","abstract":[{"lang":"eng","text":"The synthetic strigolactone (SL) analog, rac-GR24, has been instrumental in studying the role of SLs as well as karrikins because it activates the receptors DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively. Treatment with rac-GR24 modifies the root architecture at different levels, such as decreasing the lateral root density (LRD), while promoting root hair elongation or flavonol accumulation. Previously, we have shown that the flavonol biosynthesis is transcriptionally activated in the root by rac-GR24 treatment, but, thus far, the molecular players involved in that response have remained unknown. To get an in-depth insight into the changes that occur after the compound is perceived by the roots, we compared the root transcriptomes of the wild type and the more axillary growth2 (max2) mutant, affected in both SL and karrikin signaling pathways, with and without rac-GR24 treatment. Quantitative reverse transcription (qRT)-PCR, reporter line analysis and mutant phenotyping indicated that the flavonol response and the root hair elongation are controlled by the ELONGATED HYPOCOTYL 5 (HY5) and MYB12 transcription factors, but HY5, in contrast to MYB12, affects the LRD as well. Furthermore, we identified the transcription factors TARGET OF MONOPTEROS 5 (TMO5) and TMO5 LIKE1 as negative and the Mediator complex as positive regulators of the rac-GR24 effect on LRD. Altogether, hereby, we get closer toward understanding the molecular mechanisms that underlay the rac-GR24 responses in the root."}],"publication_identifier":{"eissn":["1471-9053"],"issn":["0032-0781"]},"publication":"Plant & Cell Physiology","date_created":"2021-12-28T11:44:18Z","oa_version":"Published Version","language":[{"iso":"eng"}],"quality_controlled":"1","month":"01","doi":"10.1093/pcp/pcab149","day":"21","publication_status":"published","department":[{"_id":"JiFr"}],"acknowledgement":"The authors thank Ralf Stracke (Bielefeld University, Bielefeld, Germany) for providing the myb mutants and their colleagues Bert De Rybel for the tmo5t;mo5l1 double mutant, Boris Parizot for tips on the RNA-seq analysis, Veronique Storme for statistical help on both the RNA-seq and lateral root density, and Martine De Cock for help in preparing the manuscript.","scopus_import":"1","status":"public","main_file_link":[{"url":"https://doi.org/10.1093/pcp/pcab149","open_access":"1"}],"citation":{"ieee":"S. Struk <i>et al.</i>, “Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density,” <i>Plant &#38; Cell Physiology</i>, vol. 63, no. 1. Oxford University Press, pp. 104–119, 2022.","ama":"Struk S, Braem L, Matthys C, et al. Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density. <i>Plant &#38; Cell Physiology</i>. 2022;63(1):104-119. doi:<a href=\"https://doi.org/10.1093/pcp/pcab149\">10.1093/pcp/pcab149</a>","apa":"Struk, S., Braem, L., Matthys, C., Walton, A., Vangheluwe, N., Van Praet, S., … Goormachtig, S. (2022). Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density. <i>Plant &#38; Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcab149\">https://doi.org/10.1093/pcp/pcab149</a>","chicago":"Struk, Sylwia, Lukas Braem, Cedrick Matthys, Alan Walton, Nick Vangheluwe, Stan Van Praet, Lingxiang Jiang, et al. “Transcriptional Analysis in the Arabidopsis Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation, Root Hair Elongation and Lateral Root Density.” <i>Plant &#38; Cell Physiology</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/pcp/pcab149\">https://doi.org/10.1093/pcp/pcab149</a>.","short":"S. Struk, L. Braem, C. Matthys, A. Walton, N. Vangheluwe, S. Van Praet, L. Jiang, P. Baster, C. De Cuyper, F.-D. Boyer, E. Stes, T. Beeckman, J. Friml, K. Gevaert, S. Goormachtig, Plant &#38; Cell Physiology 63 (2022) 104–119.","mla":"Struk, Sylwia, et al. “Transcriptional Analysis in the Arabidopsis Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation, Root Hair Elongation and Lateral Root Density.” <i>Plant &#38; Cell Physiology</i>, vol. 63, no. 1, Oxford University Press, 2022, pp. 104–19, doi:<a href=\"https://doi.org/10.1093/pcp/pcab149\">10.1093/pcp/pcab149</a>.","ista":"Struk S, Braem L, Matthys C, Walton A, Vangheluwe N, Van Praet S, Jiang L, Baster P, De Cuyper C, Boyer F-D, Stes E, Beeckman T, Friml J, Gevaert K, Goormachtig S. 2022. Transcriptional analysis in the Arabidopsis roots reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation and lateral root density. Plant &#38; Cell Physiology. 63(1), 104–119."},"author":[{"first_name":"Sylwia","last_name":"Struk","full_name":"Struk, Sylwia"},{"first_name":"Lukas","full_name":"Braem, Lukas","last_name":"Braem"},{"first_name":"Cedrick","full_name":"Matthys, Cedrick","last_name":"Matthys"},{"last_name":"Walton","full_name":"Walton, Alan","first_name":"Alan"},{"first_name":"Nick","full_name":"Vangheluwe, Nick","last_name":"Vangheluwe"},{"first_name":"Stan","full_name":"Van Praet, Stan","last_name":"Van Praet"},{"first_name":"Lingxiang","full_name":"Jiang, Lingxiang","last_name":"Jiang"},{"first_name":"Pawel","last_name":"Baster","id":"3028BD74-F248-11E8-B48F-1D18A9856A87","full_name":"Baster, Pawel"},{"last_name":"De Cuyper","full_name":"De Cuyper, Carolien","first_name":"Carolien"},{"first_name":"Francois-Didier","full_name":"Boyer, Francois-Didier","last_name":"Boyer"},{"full_name":"Stes, Elisabeth","last_name":"Stes","first_name":"Elisabeth"},{"full_name":"Beeckman, Tom","last_name":"Beeckman","first_name":"Tom"},{"last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"first_name":"Kris","last_name":"Gevaert","full_name":"Gevaert, Kris"},{"first_name":"Sofie","last_name":"Goormachtig","full_name":"Goormachtig, Sofie"}],"isi":1,"_id":"10583","external_id":{"pmid":["34791413"],"isi":["000877899400009"]},"issue":"1","volume":63,"intvolume":"        63","article_processing_charge":"No","page":"104-119","pmid":1,"date_published":"2022-01-21T00:00:00Z","year":"2022","publisher":"Oxford University Press","ddc":["580"]},{"abstract":[{"text":"Much of what we know about the role of auxin in plant development derives from exogenous manipulations of auxin distribution and signaling, using inhibitors, auxins and auxin analogs. In this context, synthetic auxin analogs, such as 1-Naphtalene Acetic Acid (1-NAA), are often favored over the endogenous auxin indole-3-acetic acid (IAA), in part due to their higher stability. While such auxin analogs have proven to be instrumental to reveal the various faces of auxin, they display in some cases distinct bioactivities compared to IAA. Here, we focused on the effect of auxin analogs on the accumulation of PIN proteins in Brefeldin A-sensitive endosomal aggregations (BFA bodies), and the correlation with the ability to elicit Ca 2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin-analog induced Ca 2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited a Ca 2+ response, and their differential ability to elicit Ca 2+ responses correlated partially with their ability to inhibit BFA-body formation. However, in tir1/afb and cngc14, 1-NAA-induced Ca 2+ signaling was strongly impaired, yet 1-NAA still could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent Ca 2+ signaling does not inhibit BFA body formation in Arabidopsis roots.","lang":"eng"}],"publication":"Journal of Experimental Botany","date_created":"2022-02-03T09:19:01Z","publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"type":"journal_article","date_updated":"2025-05-14T11:06:37Z","article_type":"original","title":"Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"status":"public","main_file_link":[{"open_access":"1","url":"https://biblio.ugent.be/publication/8738721"}],"citation":{"ieee":"R. Wang <i>et al.</i>, “Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots,” <i>Journal of Experimental Botany</i>, vol. 73, no. 8. Oxford University Press, 2022.","apa":"Wang, R., Himschoot, E., Grenzi, M., Chen, J., Safi, A., Krebs, M., … Vanneste, S. (2022). Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/erac019\">https://doi.org/10.1093/jxb/erac019</a>","ama":"Wang R, Himschoot E, Grenzi M, et al. Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots. <i>Journal of Experimental Botany</i>. 2022;73(8). doi:<a href=\"https://doi.org/10.1093/jxb/erac019\">10.1093/jxb/erac019</a>","mla":"Wang, R., et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal Aggregation in Arabidopsis Roots.” <i>Journal of Experimental Botany</i>, vol. 73, no. 8, erac019, Oxford University Press, 2022, doi:<a href=\"https://doi.org/10.1093/jxb/erac019\">10.1093/jxb/erac019</a>.","chicago":"Wang, R, E Himschoot, M Grenzi, J Chen, A Safi, M Krebs, K Schumacher, et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal Aggregation in Arabidopsis Roots.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/jxb/erac019\">https://doi.org/10.1093/jxb/erac019</a>.","short":"R. Wang, E. Himschoot, M. Grenzi, J. Chen, A. Safi, M. Krebs, K. Schumacher, M. Nowack, W. Moeder, K. Yoshioka, D. Van Damme, I. De Smet, D. Geelen, T. Beeckman, J. Friml, A. Costa, S. Vanneste, Journal of Experimental Botany 73 (2022).","ista":"Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack M, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J, Costa A, Vanneste S. 2022. Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany. 73(8), erac019."},"acknowledgement":"We thank Joerg Kudla (WWU Munster, Germany), Petra Dietrich (F.A. University of Erlangen-Nurnberg, Germany) for sharing published materials, and NASC for providing seeds. We thank Veronique Storme for help with the statistical analyses. Part of the imaging analysis was carried out at NOLIMITS, an advanced imaging facility established by the University of Milan.\r\nThis work was supported by grants of the China Scholarship Council (CSC) to RW and JC; Fonds Wetenschappelijk Onderzoek (FWO) to TB and (G002220N) SV; the special research fund of Ghent University to EH; the Deutsche Forschungsgemeinschaft (DFG) through Grants within FOR964 (MK and KS); Piano di Sviluppo di Ateneo 2019 (University of Milan) to AC; the European Research Council (ERC) T-Rex project 682436 to DVD; the ERC ETAP project 742985 to JF, and by a PhD fellowship from the University of Milan to MG.","scopus_import":"1","month":"04","publication_status":"published","department":[{"_id":"JiFr"}],"doi":"10.1093/jxb/erac019","ec_funded":1,"day":"18","quality_controlled":"1","oa_version":"Submitted Version","language":[{"iso":"eng"}],"intvolume":"        73","volume":73,"external_id":{"pmid":["35085386"],"isi":["000764220900001"]},"_id":"10717","issue":"8","isi":1,"author":[{"last_name":"Wang","full_name":"Wang, R","first_name":"R"},{"last_name":"Himschoot","full_name":"Himschoot, E","first_name":"E"},{"full_name":"Grenzi, M","last_name":"Grenzi","first_name":"M"},{"first_name":"J","last_name":"Chen","full_name":"Chen, J"},{"last_name":"Safi","full_name":"Safi, A","first_name":"A"},{"full_name":"Krebs, M","last_name":"Krebs","first_name":"M"},{"first_name":"K","full_name":"Schumacher, K","last_name":"Schumacher"},{"first_name":"MK","full_name":"Nowack, MK","last_name":"Nowack"},{"first_name":"W","full_name":"Moeder, W","last_name":"Moeder"},{"first_name":"K","full_name":"Yoshioka, K","last_name":"Yoshioka"},{"last_name":"Van Damme","full_name":"Van Damme, D","first_name":"D"},{"last_name":"De Smet","full_name":"De Smet, I","first_name":"I"},{"first_name":"D","last_name":"Geelen","full_name":"Geelen, D"},{"first_name":"T","last_name":"Beeckman","full_name":"Beeckman, T"},{"last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"last_name":"Costa","full_name":"Costa, A","first_name":"A"},{"first_name":"S","full_name":"Vanneste, S","last_name":"Vanneste"}],"project":[{"grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"publisher":"Oxford University Press","year":"2022","article_number":"erac019","pmid":1,"date_published":"2022-04-18T00:00:00Z","article_processing_charge":"No"},{"_id":"10719","external_id":{"isi":["000761281200011"],"pmid":["35018726"]},"issue":"2","author":[{"first_name":"Z","full_name":"Yu, Z","last_name":"Yu"},{"full_name":"Zhang, F","last_name":"Zhang","first_name":"F"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Z","last_name":"Ding","full_name":"Ding, Z"}],"isi":1,"intvolume":"        64","volume":64,"date_published":"2022-02-01T00:00:00Z","pmid":1,"page":"371-392","article_processing_charge":"No","ddc":["580"],"publisher":"Wiley","year":"2022","title":"Auxin signaling: Research advances over the past 30 years","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"review","oa":1,"publication":"Journal of Integrative Plant Biology","date_created":"2022-02-03T09:52:59Z","publication_identifier":{"eissn":["1744-7909"],"issn":["1672-9072"]},"abstract":[{"lang":"eng","text":"Auxin, one of the first identified and most widely studied phytohormones, has been and will remain a hot topic in plant biology. After more than a century of passionate exploration, the mysteries of its synthesis, transport, signaling, and metabolism have largely been unlocked. Due to the rapid development of new technologies, new methods, and new genetic materials, the study of auxin has entered the fast lane over the past 30 years. Here, we highlight advances in understanding auxin signaling, including auxin perception, rapid auxin responses, TRANSPORT INHIBITOR RESPONSE 1 and AUXIN SIGNALING F-boxes (TIR1/AFBs)-mediated transcriptional and non-transcriptional branches, and the epigenetic regulation of auxin signaling. We also focus on feedback inhibition mechanisms that prevent the over-amplification of auxin signals. In addition, we cover the TRANSMEMBRANE KINASEs (TMKs)-mediated non-canonical signaling, which converges with TIR1/AFBs-mediated transcriptional regulation to coordinate plant growth and development. The identification of additional auxin signaling components and their regulation will continue to open new avenues of research in this field, leading to an increasingly deeper, more comprehensive understanding of how auxin signals are interpreted at the cellular level to regulate plant growth and development."}],"type":"journal_article","date_updated":"2026-06-18T08:47:21Z","department":[{"_id":"JiFr"}],"publication_status":"published","day":"01","doi":"10.1111/jipb.13225","corr_author":"1","month":"02","quality_controlled":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"citation":{"mla":"Yu, Z., et al. “Auxin Signaling: Research Advances over the Past 30 Years.” <i>Journal of Integrative Plant Biology</i>, vol. 64, no. 2, Wiley, 2022, pp. 371–92, doi:<a href=\"https://doi.org/10.1111/jipb.13225\">10.1111/jipb.13225</a>.","chicago":"Yu, Z, F Zhang, Jiří Friml, and Z Ding. “Auxin Signaling: Research Advances over the Past 30 Years.” <i>Journal of Integrative Plant Biology</i>. Wiley, 2022. <a href=\"https://doi.org/10.1111/jipb.13225\">https://doi.org/10.1111/jipb.13225</a>.","short":"Z. Yu, F. Zhang, J. Friml, Z. Ding, Journal of Integrative Plant Biology 64 (2022) 371–392.","ista":"Yu Z, Zhang F, Friml J, Ding Z. 2022. Auxin signaling: Research advances over the past 30 years. Journal of Integrative Plant Biology. 64(2), 371–392.","ieee":"Z. Yu, F. Zhang, J. Friml, and Z. Ding, “Auxin signaling: Research advances over the past 30 years,” <i>Journal of Integrative Plant Biology</i>, vol. 64, no. 2. Wiley, pp. 371–392, 2022.","ama":"Yu Z, Zhang F, Friml J, Ding Z. Auxin signaling: Research advances over the past 30 years. <i>Journal of Integrative Plant Biology</i>. 2022;64(2):371-392. doi:<a href=\"https://doi.org/10.1111/jipb.13225\">10.1111/jipb.13225</a>","apa":"Yu, Z., Zhang, F., Friml, J., &#38; Ding, Z. (2022). Auxin signaling: Research advances over the past 30 years. <i>Journal of Integrative Plant Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/jipb.13225\">https://doi.org/10.1111/jipb.13225</a>"},"status":"public","main_file_link":[{"url":"https://doi.org/10.1111/jipb.13225","open_access":"1"}],"acknowledgement":"This research was financially supported by the National Natural Science Foundation of China and the Israel Science Foundation (NSFC-ISF; 32061143005), National Natural Science Foundation of China (32000225), Natural Science Foundation of Shandong Province (ZR2020QC036), and China Postdoctoral Science Foundation (2020M682165).\r\n","scopus_import":"1"},{"language":[{"iso":"eng"}],"quality_controlled":"1","oa_version":"Published Version","department":[{"_id":"JiFr"}],"publication_status":"published","day":"01","doi":"10.1016/j.pbi.2022.102174","month":"02","corr_author":"1","acknowledgement":"The authors apologize to those researchers whose work was not cited. In addition, exciting topics such as PIN polarization in context of phyllotaxis, shoot branching and termination of gravitropic bending, or role of additional auxin transporters could not have been included owing to lack of space. This work was supported by the Czech Science Foundation GAČR (GA18-26981S). The authors also acknowledge the EMBO for supporting J.H. with a long-term fellowship (ALTF217-2021).","scopus_import":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"date_updated":"2022-03-10T13:34:09Z","creator":"dernst","file_size":820322,"date_created":"2022-03-10T13:34:09Z","file_id":"10844","file_name":"2022_CurrentOpPlantBiology_Hajny.pdf","content_type":"application/pdf","checksum":"f1ee02b6fb4200934eeb31fa69120885","relation":"main_file","access_level":"open_access","success":1}],"citation":{"apa":"Hajny, J., Tan, S., &#38; Friml, J. (2022). Auxin canalization: From speculative models toward molecular players. <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2022.102174\">https://doi.org/10.1016/j.pbi.2022.102174</a>","ama":"Hajny J, Tan S, Friml J. Auxin canalization: From speculative models toward molecular players. <i>Current Opinion in Plant Biology</i>. 2022;65(2). doi:<a href=\"https://doi.org/10.1016/j.pbi.2022.102174\">10.1016/j.pbi.2022.102174</a>","ieee":"J. Hajny, S. Tan, and J. Friml, “Auxin canalization: From speculative models toward molecular players,” <i>Current Opinion in Plant Biology</i>, vol. 65, no. 2. Elsevier, 2022.","ista":"Hajny J, Tan S, Friml J. 2022. Auxin canalization: From speculative models toward molecular players. Current Opinion in Plant Biology. 65(2), 102174.","chicago":"Hajny, Jakub, Shutang Tan, and Jiří Friml. “Auxin Canalization: From Speculative Models toward Molecular Players.” <i>Current Opinion in Plant Biology</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.pbi.2022.102174\">https://doi.org/10.1016/j.pbi.2022.102174</a>.","mla":"Hajny, Jakub, et al. “Auxin Canalization: From Speculative Models toward Molecular Players.” <i>Current Opinion in Plant Biology</i>, vol. 65, no. 2, 102174, Elsevier, 2022, doi:<a href=\"https://doi.org/10.1016/j.pbi.2022.102174\">10.1016/j.pbi.2022.102174</a>.","short":"J. Hajny, S. Tan, J. Friml, Current Opinion in Plant Biology 65 (2022)."},"status":"public","has_accepted_license":"1","oa":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"Auxin canalization: From speculative models toward molecular players","article_type":"original","type":"journal_article","date_updated":"2024-10-09T21:01:37Z","file_date_updated":"2022-03-10T13:34:09Z","date_created":"2022-02-20T23:01:32Z","publication":"Current Opinion in Plant Biology","publication_identifier":{"issn":["1369-5266"]},"abstract":[{"text":"Among the most fascinated properties of the plant hormone auxin is its ability to promote formation of its own directional transport routes. These gradually narrowing auxin channels form from the auxin source toward the sink and involve coordinated, collective polarization of individual cells. Once established, the channels provide positional information, along which new vascular strands form, for example, during organogenesis, regeneration, or leave venation. The main prerequisite of this still mysterious auxin canalization mechanism is a feedback between auxin signaling and its directional transport. This is manifested by auxin-induced re-arrangements of polar, subcellular localization of PIN-FORMED (PIN) auxin exporters. Immanent open questions relate to how position of auxin source and sink as well as tissue context are sensed and translated into tissue polarization and how cells communicate to polarize coordinately. Recently, identification of the first molecular players opens new avenues into molecular studies of this intriguing example of self-organizing plant development.","lang":"eng"}],"article_processing_charge":"Yes (via OA deal)","date_published":"2022-02-01T00:00:00Z","pmid":1,"year":"2022","article_number":"102174","ddc":["580"],"publisher":"Elsevier","isi":1,"author":[{"first_name":"Jakub","orcid":"0000-0003-2140-7195","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","full_name":"Hajny, Jakub","last_name":"Hajny"},{"id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","last_name":"Tan","first_name":"Shutang","orcid":"0000-0002-0471-8285"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"issue":"2","_id":"10768","external_id":{"isi":["000758724700004"],"pmid":["35123880"]},"volume":65,"intvolume":"        65"},{"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"ddc":["580"],"citation":{"ista":"Johnson AJ. 2021. Raw data from Johnson et al, PNAS, 2021, Zenodo, <a href=\"https://doi.org/10.5281/ZENODO.5747100\">10.5281/ZENODO.5747100</a>.","chicago":"Johnson, Alexander J. “Raw Data from Johnson et Al, PNAS, 2021.” Zenodo, 2021. <a href=\"https://doi.org/10.5281/ZENODO.5747100\">https://doi.org/10.5281/ZENODO.5747100</a>.","short":"A.J. Johnson, (2021).","mla":"Johnson, Alexander J. <i>Raw Data from Johnson et Al, PNAS, 2021</i>. Zenodo, 2021, doi:<a href=\"https://doi.org/10.5281/ZENODO.5747100\">10.5281/ZENODO.5747100</a>.","apa":"Johnson, A. J. (2021). Raw data from Johnson et al, PNAS, 2021. Zenodo. <a href=\"https://doi.org/10.5281/ZENODO.5747100\">https://doi.org/10.5281/ZENODO.5747100</a>","ama":"Johnson AJ. Raw data from Johnson et al, PNAS, 2021. 2021. doi:<a href=\"https://doi.org/10.5281/ZENODO.5747100\">10.5281/ZENODO.5747100</a>","ieee":"A. J. Johnson, “Raw data from Johnson et al, PNAS, 2021.” Zenodo, 2021."},"main_file_link":[{"url":"https://doi.org/10.5281/zenodo.5747100","open_access":"1"}],"status":"public","publisher":"Zenodo","year":"2021","department":[{"_id":"JiFr"}],"doi":"10.5281/ZENODO.5747100","day":"01","related_material":{"record":[{"status":"public","relation":"used_in_publication","id":"9887"}]},"date_published":"2021-12-01T00:00:00Z","month":"12","corr_author":"1","oa_version":"Published Version","article_processing_charge":"No","date_created":"2024-02-14T14:13:48Z","abstract":[{"text":"Raw data generated from the publication - The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis by Johnson et al., 2021 In PNAS","lang":"eng"}],"type":"research_data_reference","date_updated":"2025-05-14T09:25:33Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Raw data from Johnson et al, PNAS, 2021","_id":"14988","has_accepted_license":"1","oa":1,"author":[{"orcid":"0000-0002-2739-8843","first_name":"Alexander J","full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson"}]},{"file":[{"file_size":4738995,"creator":"dernst","date_updated":"2024-04-29T06:51:59Z","file_name":"2021_PlosOne_Overdijk.pdf","date_created":"2024-04-29T06:51:59Z","file_id":"15349","relation":"main_file","checksum":"25b7b329435af57db2c95571a8ef32fe","content_type":"application/pdf","success":1,"access_level":"open_access"}],"citation":{"chicago":"Overdijk, Elysa J. R., Vera Putker, Joep Smits, Han Tang, Klaas Bouwmeester, Francine Govers, and Tijs Ketelaar. “Phytophthora Infestans RXLR Effector AVR1 Disturbs the Growth of Physcomitrium Patens without Affecting Sec5 Localization.” <i>PLoS One</i>. Public Library of Science, 2021. <a href=\"https://doi.org/10.1371/journal.pone.0249637\">https://doi.org/10.1371/journal.pone.0249637</a>.","short":"E.J.R. Overdijk, V. Putker, J. Smits, H. Tang, K. Bouwmeester, F. Govers, T. Ketelaar, PLoS One 16 (2021).","mla":"Overdijk, Elysa J. R., et al. “Phytophthora Infestans RXLR Effector AVR1 Disturbs the Growth of Physcomitrium Patens without Affecting Sec5 Localization.” <i>PLoS One</i>, vol. 16, no. 4, e0249637, Public Library of Science, 2021, doi:<a href=\"https://doi.org/10.1371/journal.pone.0249637\">10.1371/journal.pone.0249637</a>.","ista":"Overdijk EJR, Putker V, Smits J, Tang H, Bouwmeester K, Govers F, Ketelaar T. 2021. Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium patens without affecting Sec5 localization. PLoS One. 16(4), e0249637.","ieee":"E. J. R. Overdijk <i>et al.</i>, “Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium patens without affecting Sec5 localization,” <i>PLoS One</i>, vol. 16, no. 4. Public Library of Science, 2021.","ama":"Overdijk EJR, Putker V, Smits J, et al. Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium patens without affecting Sec5 localization. <i>PLoS One</i>. 2021;16(4). doi:<a href=\"https://doi.org/10.1371/journal.pone.0249637\">10.1371/journal.pone.0249637</a>","apa":"Overdijk, E. J. R., Putker, V., Smits, J., Tang, H., Bouwmeester, K., Govers, F., &#38; Ketelaar, T. (2021). Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium patens without affecting Sec5 localization. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0249637\">https://doi.org/10.1371/journal.pone.0249637</a>"},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"status":"public","quality_controlled":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"doi":"10.1371/journal.pone.0249637","day":"08","department":[{"_id":"JiFr"}],"publication_status":"published","month":"04","date_updated":"2024-04-29T06:53:15Z","type":"journal_article","file_date_updated":"2024-04-29T06:51:59Z","publication_identifier":{"issn":["1932-6203"]},"date_created":"2024-04-03T07:38:14Z","publication":"PLoS One","abstract":[{"lang":"eng","text":"Plant pathogens often exploit a whole range of effectors to facilitate infection. The RXLR effector AVR1 produced by the oomycete plant pathogen Phytophthora infestans suppresses host defense by targeting Sec5. Sec5 is a subunit of the exocyst, a protein complex that is important for mediating polarized exocytosis during plant development and defense against pathogens. The mechanism by which AVR1 manipulates Sec5 functioning is unknown. In this study, we analyzed the effect of AVR1 on Sec5 localization and functioning in the moss Physcomitrium patens. P. patens has four Sec5 homologs. Two (PpSec5b and PpSec5d) were found to interact with AVR1 in yeast-two-hybrid assays while none of the four showed a positive interaction with AVR1ΔT, a truncated version of AVR1. In P. patens lines carrying β-estradiol inducible AVR1 or AVR1ΔT transgenes, expression of AVR1 or AVR1ΔT caused defects in the development of caulonemal protonema cells and abnormal morphology of chloronema cells. Similar phenotypes were observed in Sec5- or Sec6-silenced P. patens lines, suggesting that both AVR1 and AVR1ΔT affect exocyst functioning in P. patens. With respect to Sec5 localization we found no differences between β-estradiol-treated and untreated transgenic AVR1 lines. Sec5 localizes at the plasma membrane in growing caulonema cells, also during pathogen attack, and its subcellular localization is the same, with or without AVR1 in the vicinity."}],"oa":1,"has_accepted_license":"1","keyword":["Multidisciplinary"],"title":"Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium patens without affecting Sec5 localization","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","article_number":"e0249637","year":"2021","ddc":["580"],"publisher":"Public Library of Science","article_processing_charge":"Yes","pmid":1,"date_published":"2021-04-08T00:00:00Z","volume":16,"intvolume":"        16","author":[{"full_name":"Overdijk, Elysa J. R.","last_name":"Overdijk","first_name":"Elysa J. R."},{"first_name":"Vera","full_name":"Putker, Vera","last_name":"Putker"},{"first_name":"Joep","full_name":"Smits, Joep","last_name":"Smits"},{"first_name":"Han","orcid":"0000-0001-6152-6637","last_name":"Tang","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","full_name":"Tang, Han"},{"full_name":"Bouwmeester, Klaas","last_name":"Bouwmeester","first_name":"Klaas"},{"first_name":"Francine","full_name":"Govers, Francine","last_name":"Govers"},{"last_name":"Ketelaar","full_name":"Ketelaar, Tijs","first_name":"Tijs"}],"_id":"15266","issue":"4","external_id":{"pmid":["33831039"]}},{"article_processing_charge":"Yes","pmid":1,"date_published":"2021-06-24T00:00:00Z","year":"2021","article_number":"e1009641","ddc":["580"],"publisher":"Public Library of Science","author":[{"first_name":"Fernando","full_name":"Navarrete, Fernando","last_name":"Navarrete"},{"first_name":"Nenad","full_name":"Grujic, Nenad","last_name":"Grujic"},{"first_name":"Alexandra","last_name":"Stirnberg","full_name":"Stirnberg, Alexandra"},{"last_name":"Saado","full_name":"Saado, Indira","first_name":"Indira"},{"full_name":"Aleksza, David","last_name":"Aleksza","first_name":"David"},{"first_name":"Michelle C","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","last_name":"Gallei"},{"first_name":"Hazem","last_name":"Adi","full_name":"Adi, Hazem"},{"first_name":"André","full_name":"Alcântara, André","last_name":"Alcântara"},{"first_name":"Mamoona","last_name":"Khan","full_name":"Khan, Mamoona"},{"first_name":"Janos","last_name":"Bindics","full_name":"Bindics, Janos"},{"first_name":"Marco","full_name":"Trujillo, Marco","last_name":"Trujillo"},{"first_name":"Armin","last_name":"Djamei","full_name":"Djamei, Armin"}],"_id":"15276","issue":"6","external_id":{"pmid":["34166468"]},"volume":17,"intvolume":"        17","oa_version":"Published Version","quality_controlled":"1","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"publication_status":"published","doi":"10.1371/journal.ppat.1009641","day":"24","month":"06","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"citation":{"apa":"Navarrete, F., Grujic, N., Stirnberg, A., Saado, I., Aleksza, D., Gallei, M. C., … Djamei, A. (2021). The Pleiades are a cluster of fungal effectors that inhibit host defenses. <i>PLOS Pathogens</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.ppat.1009641\">https://doi.org/10.1371/journal.ppat.1009641</a>","ama":"Navarrete F, Grujic N, Stirnberg A, et al. The Pleiades are a cluster of fungal effectors that inhibit host defenses. <i>PLOS Pathogens</i>. 2021;17(6). doi:<a href=\"https://doi.org/10.1371/journal.ppat.1009641\">10.1371/journal.ppat.1009641</a>","ieee":"F. Navarrete <i>et al.</i>, “The Pleiades are a cluster of fungal effectors that inhibit host defenses,” <i>PLOS Pathogens</i>, vol. 17, no. 6. Public Library of Science, 2021.","ista":"Navarrete F, Grujic N, Stirnberg A, Saado I, Aleksza D, Gallei MC, Adi H, Alcântara A, Khan M, Bindics J, Trujillo M, Djamei A. 2021. The Pleiades are a cluster of fungal effectors that inhibit host defenses. PLOS Pathogens. 17(6), e1009641.","short":"F. Navarrete, N. Grujic, A. Stirnberg, I. Saado, D. Aleksza, M.C. Gallei, H. Adi, A. Alcântara, M. Khan, J. Bindics, M. Trujillo, A. Djamei, PLOS Pathogens 17 (2021).","mla":"Navarrete, Fernando, et al. “The Pleiades Are a Cluster of Fungal Effectors That Inhibit Host Defenses.” <i>PLOS Pathogens</i>, vol. 17, no. 6, e1009641, Public Library of Science, 2021, doi:<a href=\"https://doi.org/10.1371/journal.ppat.1009641\">10.1371/journal.ppat.1009641</a>.","chicago":"Navarrete, Fernando, Nenad Grujic, Alexandra Stirnberg, Indira Saado, David Aleksza, Michelle C Gallei, Hazem Adi, et al. “The Pleiades Are a Cluster of Fungal Effectors That Inhibit Host Defenses.” <i>PLOS Pathogens</i>. Public Library of Science, 2021. <a href=\"https://doi.org/10.1371/journal.ppat.1009641\">https://doi.org/10.1371/journal.ppat.1009641</a>."},"file":[{"success":1,"access_level":"open_access","content_type":"application/pdf","relation":"main_file","checksum":"ab8428291a0c14607c4ea5656c029cff","file_id":"15305","date_created":"2024-04-09T10:24:43Z","file_name":"2021_PlosPathogens_Navarrete.pdf","date_updated":"2024-04-09T10:24:43Z","file_size":2616563,"creator":"dernst"}],"status":"public","oa":1,"has_accepted_license":"1","keyword":["Virology","Genetics","Molecular Biology","Immunology","Microbiology","Parasitology"],"title":"The Pleiades are a cluster of fungal effectors that inhibit host defenses","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","type":"journal_article","date_updated":"2024-04-09T10:26:12Z","file_date_updated":"2024-04-09T10:24:43Z","date_created":"2024-04-03T08:00:34Z","publication":"PLOS Pathogens","publication_identifier":{"issn":["1553-7374"]},"abstract":[{"text":"Biotrophic plant pathogens secrete effector proteins to manipulate the host physiology. Effectors suppress defenses and induce an environment favorable to disease development. Sequence-based prediction of effector function is impeded by their rapid evolution rate. In the maize pathogen <jats:italic>Ustilago maydis</jats:italic>, effector-coding genes frequently organize in clusters. Here we describe the functional characterization of the <jats:italic>pleiades</jats:italic>, a cluster of ten effector genes, by analyzing the micro- and macroscopic phenotype of the cluster deletion and expressing these proteins <jats:italic>in planta</jats:italic>. Deletion of the <jats:italic>pleiades</jats:italic> leads to strongly impaired virulence and accumulation of reactive oxygen species (ROS) in infected tissue. Eight of the Pleiades suppress the production of ROS upon perception of pathogen associated molecular patterns (PAMPs). Although functionally redundant, the Pleiades target different host components. The paralogs Taygeta1 and Merope1 suppress ROS production in either the cytoplasm or nucleus, respectively. Merope1 targets and promotes the auto-ubiquitination activity of RFI2, a conserved family of E3 ligases that regulates the production of PAMP-triggered ROS burst in plants.","lang":"eng"}]},{"intvolume":"       599","volume":599,"_id":"10223","issue":"7884","external_id":{"pmid":["34707283"],"isi":["000713338100006"]},"isi":1,"author":[{"orcid":"0000-0002-5607-272X","first_name":"Lanxin","last_name":"Li","full_name":"Li, Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-7241-2328","first_name":"Inge","full_name":"Verstraeten, Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten"},{"first_name":"Mark","last_name":"Roosjen","full_name":"Roosjen, Mark"},{"last_name":"Takahashi","full_name":"Takahashi, Koji","first_name":"Koji"},{"last_name":"Rodriguez Solovey","full_name":"Rodriguez Solovey, Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7244-7237","first_name":"Lesia"},{"full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609","first_name":"Jack"},{"first_name":"Jian","full_name":"Chen, Jian","last_name":"Chen"},{"last_name":"Shabala","full_name":"Shabala, Lana","first_name":"Lana"},{"last_name":"Smet","full_name":"Smet, Wouter","first_name":"Wouter"},{"full_name":"Ren, Hong","last_name":"Ren","first_name":"Hong"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"},{"last_name":"Shabala","full_name":"Shabala, Sergey","first_name":"Sergey"},{"first_name":"Bert","last_name":"De Rybel","full_name":"De Rybel, Bert"},{"first_name":"Dolf","last_name":"Weijers","full_name":"Weijers, Dolf"},{"first_name":"Toshinori","last_name":"Kinoshita","full_name":"Kinoshita, Toshinori"},{"full_name":"Gray, William M.","last_name":"Gray","first_name":"William M."},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"publisher":"Springer Nature","project":[{"grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425"},{"grant_number":"665385","call_identifier":"H2020","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"_id":"26B4D67E-B435-11E9-9278-68D0E5697425","grant_number":"25351","name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root"}],"year":"2021","date_published":"2021-11-11T00:00:00Z","pmid":1,"page":"273-277","article_processing_charge":"No","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"publication":"Nature","date_created":"2021-11-07T23:01:25Z","abstract":[{"lang":"eng","text":"Growth regulation tailors development in plants to their environment. A prominent example of this is the response to gravity, in which shoots bend up and roots bend down1. This paradox is based on opposite effects of the phytohormone auxin, which promotes cell expansion in shoots while inhibiting it in roots via a yet unknown cellular mechanism2. Here, by combining microfluidics, live imaging, genetic engineering and phosphoproteomics in Arabidopsis thaliana, we advance understanding of how auxin inhibits root growth. We show that auxin activates two distinct, antagonistically acting signalling pathways that converge on rapid regulation of apoplastic pH, a causative determinant of growth. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts with and mediates phosphorylation and activation of plasma membrane H+-ATPases for apoplast acidification, while intracellular canonical auxin signalling promotes net cellular H+ influx, causing apoplast alkalinization. Simultaneous activation of these two counteracting mechanisms poises roots for rapid, fine-tuned growth modulation in navigating complex soil environments."}],"date_updated":"2025-07-10T11:49:46Z","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth","article_type":"original","oa":1,"keyword":["Multidisciplinary"],"citation":{"apa":"Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez Solovey, L., Merrin, J., … Friml, J. (2021). Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-021-04037-6\">https://doi.org/10.1038/s41586-021-04037-6</a>","ama":"Li L, Verstraeten I, Roosjen M, et al. Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth. <i>Nature</i>. 2021;599(7884):273-277. doi:<a href=\"https://doi.org/10.1038/s41586-021-04037-6\">10.1038/s41586-021-04037-6</a>","ieee":"L. Li <i>et al.</i>, “Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth,” <i>Nature</i>, vol. 599, no. 7884. Springer Nature, pp. 273–277, 2021.","ista":"Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez Solovey L, Merrin J, Chen J, Shabala L, Smet W, Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D, Kinoshita T, Gray WM, Friml J. 2021. Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth. Nature. 599(7884), 273–277.","short":"L. Li, I. Verstraeten, M. Roosjen, K. Takahashi, L. Rodriguez Solovey, J. Merrin, J. Chen, L. Shabala, W. Smet, H. Ren, S. Vanneste, S. Shabala, B. De Rybel, D. Weijers, T. Kinoshita, W.M. Gray, J. Friml, Nature 599 (2021) 273–277.","mla":"Li, Lanxin, et al. “Cell Surface and Intracellular Auxin Signalling for H<sup>+</sup> Fluxes in Root Growth.” <i>Nature</i>, vol. 599, no. 7884, Springer Nature, 2021, pp. 273–77, doi:<a href=\"https://doi.org/10.1038/s41586-021-04037-6\">10.1038/s41586-021-04037-6</a>.","chicago":"Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez Solovey, Jack Merrin, Jian Chen, et al. “Cell Surface and Intracellular Auxin Signalling for H<sup>+</sup> Fluxes in Root Growth.” <i>Nature</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41586-021-04037-6\">https://doi.org/10.1038/s41586-021-04037-6</a>."},"status":"public","main_file_link":[{"open_access":"1","url":"https://www.doi.org/10.21203/rs.3.rs-266395/v3"}],"acknowledgement":"We thank N. Gnyliukh and L. Hörmayer for technical assistance and N. Paris for sharing PM-Cyto seeds. We gratefully acknowledge the Life Science, Machine Shop and Bioimaging Facilities of IST Austria. This project has received funding from the European Research Council Advanced Grant (ETAP-742985) and the Austrian Science Fund (FWF) under I 3630-B25 to J.F., the National Institutes of Health (GM067203) to W.M.G., the Netherlands Organization for Scientific Research (NWO; VIDI-864.13.001), Research Foundation-Flanders (FWO; Odysseus II G0D0515N) and a European Research Council Starting Grant (TORPEDO-714055) to W.S. and B.D.R., the VICI grant (865.14.001) from the Netherlands Organization for Scientific Research to M.R. and D.W., the Australian Research Council and China National Distinguished Expert Project (WQ20174400441) to S.S., the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and 20H05910), the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement no. 665385 and the DOC Fellowship of the Austrian Academy of Sciences to L.L., and the China Scholarship Council to J.C.","scopus_import":"1","doi":"10.1038/s41586-021-04037-6","day":"11","ec_funded":1,"department":[{"_id":"JiFr"},{"_id":"NanoFab"}],"publication_status":"published","month":"11","corr_author":"1","related_material":{"record":[{"status":"public","relation":"earlier_version","id":"10095"}],"link":[{"description":"News on IST Webpage","relation":"press_release","url":"https://ist.ac.at/en/news/stop-and-grow/"}]},"oa_version":"Preprint","language":[{"iso":"eng"}],"quality_controlled":"1","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"Bio"}]},{"oa_version":"None","language":[{"iso":"eng"}],"quality_controlled":"1","ec_funded":1,"doi":"10.1007/978-1-0716-1677-2_2","day":"14","department":[{"_id":"JiFr"}],"publication_status":"published","corr_author":"1","month":"10","acknowledgement":"The Ceratopteris richardii spores were obtained from the lab of Jo Ann Banks at Purdue University. This work was supported by funding from the European Union’s Horizon 2020 research and innovation program (ERC grant agreement number 742985), Austrian Science Fund (FWF, grant number I 3630-B25), IST Fellow program and DOC Fellowship of the Austrian Academy of Sciences.","scopus_import":"1","citation":{"ista":"Zhang Y, Li L, Friml J. 2021.Evaluation of gravitropism in non-seed plants. In: Plant Gravitropism. Methods in Molecular Biology, vol. 2368, 43–51.","short":"Y. Zhang, L. Li, J. Friml, in:, E.B. Blancaflor (Ed.), Plant Gravitropism, Springer Nature, 2021, pp. 43–51.","chicago":"Zhang, Yuzhou, Lanxin Li, and Jiří Friml. “Evaluation of Gravitropism in Non-Seed Plants.” In <i>Plant Gravitropism</i>, edited by Elison B Blancaflor, 2368:43–51. MIMB. Springer Nature, 2021. <a href=\"https://doi.org/10.1007/978-1-0716-1677-2_2\">https://doi.org/10.1007/978-1-0716-1677-2_2</a>.","mla":"Zhang, Yuzhou, et al. “Evaluation of Gravitropism in Non-Seed Plants.” <i>Plant Gravitropism</i>, edited by Elison B Blancaflor, vol. 2368, Springer Nature, 2021, pp. 43–51, doi:<a href=\"https://doi.org/10.1007/978-1-0716-1677-2_2\">10.1007/978-1-0716-1677-2_2</a>.","apa":"Zhang, Y., Li, L., &#38; Friml, J. (2021). Evaluation of gravitropism in non-seed plants. In E. B. Blancaflor (Ed.), <i>Plant Gravitropism</i> (Vol. 2368, pp. 43–51). Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-1677-2_2\">https://doi.org/10.1007/978-1-0716-1677-2_2</a>","ama":"Zhang Y, Li L, Friml J. Evaluation of gravitropism in non-seed plants. In: Blancaflor EB, ed. <i>Plant Gravitropism</i>. Vol 2368. MIMB. Springer Nature; 2021:43-51. doi:<a href=\"https://doi.org/10.1007/978-1-0716-1677-2_2\">10.1007/978-1-0716-1677-2_2</a>","ieee":"Y. Zhang, L. Li, and J. Friml, “Evaluation of gravitropism in non-seed plants,” in <i>Plant Gravitropism</i>, vol. 2368, E. B. Blancaflor, Ed. Springer Nature, 2021, pp. 43–51."},"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Evaluation of gravitropism in non-seed plants","date_updated":"2025-04-14T07:45:00Z","type":"book_chapter","publication_identifier":{"eisbn":["978-1-0716-1677-2"],"isbn":["978-1-0716-1676-5"]},"publication":"Plant Gravitropism","date_created":"2021-11-11T09:26:10Z","abstract":[{"lang":"eng","text":"Tropisms are among the most important growth responses for plant adaptation to the surrounding environment. One of the most common tropisms is root gravitropism. Root gravitropism enables the plant to anchor securely to the soil enabling the absorption of water and nutrients. Most of the knowledge related to the plant gravitropism has been acquired from the flowering plants, due to limited research in non-seed plants. Limited research on non-seed plants is due in large part to the lack of standard research methods. Here, we describe the experimental methods to evaluate gravitropism in representative non-seed plant species, including the non-vascular plant moss Physcomitrium patens, the early diverging extant vascular plant lycophyte Selaginella moellendorffii and fern Ceratopteris richardii. In addition, we introduce the methods used for statistical analysis of the root gravitropism in non-seed plant species."}],"series_title":"MIMB","page":"43-51","article_processing_charge":"No","pmid":1,"date_published":"2021-10-14T00:00:00Z","editor":[{"full_name":"Blancaflor, Elison B","last_name":"Blancaflor","first_name":"Elison B"}],"year":"2021","publisher":"Springer Nature","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985"},{"grant_number":"291734","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630"}],"author":[{"last_name":"Zhang","full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956","first_name":"Yuzhou"},{"orcid":"0000-0002-5607-272X","first_name":"Lanxin","last_name":"Li","full_name":"Li, Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"_id":"10267","external_id":{"pmid":["34647246"]},"alternative_title":["Methods in Molecular Biology"],"volume":2368,"intvolume":"      2368"},{"date_updated":"2022-06-03T06:47:06Z","type":"book_chapter","publication_identifier":{"eisbn":["978-1-0716-1744-1"],"eissn":["1940-6029"],"isbn":["978-1-0716-1743-4"],"issn":["1064-3745"]},"publication":"Plant Cell Division","date_created":"2021-11-11T10:03:30Z","abstract":[{"text":"The analysis of dynamic cellular processes such as plant cytokinesis stands and falls with live-cell time-lapse confocal imaging. Conventional approaches to time-lapse imaging of cell division in Arabidopsis root tips are tedious and have low throughput. Here, we describe a protocol for long-term time-lapse simultaneous imaging of multiple root tips on a vertical-stage confocal microscope with automated root tracking. We also provide modifications of the basic protocol to implement this imaging method in the analysis of genetic, pharmacological or laser ablation wounding-mediated experimental manipulations. Our method dramatically improves the efficiency of cell division time-lapse imaging by increasing the throughput, while reducing the person-hour requirements of such experiments.","lang":"eng"}],"series_title":"MIMB","title":"Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"We thank B. De Rybel for allowing M.G. to work on this manuscript during a postdoc in his laboratory, and EMBO for supporting M.G. with a Long-Term fellowship (ALTF 1005-2019) during this time. We acknowledge the service and support by the Bioimaging Facility at IST Austria, and finally, we thank A. Mally for proofreading and correcting the manuscript.","scopus_import":"1","citation":{"ama":"Hörmayer L, Friml J, Glanc M. Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy. In: <i>Plant Cell Division</i>. Vol 2382. MIMB. Humana Press; 2021:105-114. doi:<a href=\"https://doi.org/10.1007/978-1-0716-1744-1_6\">10.1007/978-1-0716-1744-1_6</a>","apa":"Hörmayer, L., Friml, J., &#38; Glanc, M. (2021). Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy. In <i>Plant Cell Division</i> (Vol. 2382, pp. 105–114). Humana Press. <a href=\"https://doi.org/10.1007/978-1-0716-1744-1_6\">https://doi.org/10.1007/978-1-0716-1744-1_6</a>","ieee":"L. Hörmayer, J. Friml, and M. Glanc, “Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy,” in <i>Plant Cell Division</i>, vol. 2382, Humana Press, 2021, pp. 105–114.","ista":"Hörmayer L, Friml J, Glanc M. 2021.Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy. In: Plant Cell Division. Methods in Molecular Biology, vol. 2382, 105–114.","chicago":"Hörmayer, Lukas, Jiří Friml, and Matous Glanc. “Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy.” In <i>Plant Cell Division</i>, 2382:105–14. MIMB. Humana Press, 2021. <a href=\"https://doi.org/10.1007/978-1-0716-1744-1_6\">https://doi.org/10.1007/978-1-0716-1744-1_6</a>.","short":"L. Hörmayer, J. Friml, M. Glanc, in:, Plant Cell Division, Humana Press, 2021, pp. 105–114.","mla":"Hörmayer, Lukas, et al. “Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy.” <i>Plant Cell Division</i>, vol. 2382, Humana Press, 2021, pp. 105–14, doi:<a href=\"https://doi.org/10.1007/978-1-0716-1744-1_6\">10.1007/978-1-0716-1744-1_6</a>."},"status":"public","quality_controlled":"1","oa_version":"None","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"}],"day":"28","doi":"10.1007/978-1-0716-1744-1_6","publication_status":"published","department":[{"_id":"JiFr"}],"month":"10","volume":2382,"intvolume":"      2382","author":[{"first_name":"Lukas","last_name":"Hörmayer","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","full_name":"Hörmayer, Lukas"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"},{"last_name":"Glanc","full_name":"Glanc, Matous","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","orcid":"0000-0003-0619-7783","first_name":"Matous"}],"external_id":{"pmid":["34705235"]},"_id":"10268","alternative_title":["Methods in Molecular Biology"],"year":"2021","publisher":"Humana Press","page":"105-114","article_processing_charge":"No","pmid":1,"date_published":"2021-10-28T00:00:00Z"},{"_id":"10326","external_id":{"pmid":["34764442"],"isi":["000717408000002"]},"author":[{"first_name":"Enjun","full_name":"Xu, Enjun","last_name":"Xu"},{"first_name":"Liang","full_name":"Chai, Liang","last_name":"Chai"},{"full_name":"Zhang, Shiqi","last_name":"Zhang","first_name":"Shiqi"},{"full_name":"Yu, Ruixue","last_name":"Yu","first_name":"Ruixue"},{"orcid":"0000-0001-7048-4627","first_name":"Xixi","full_name":"Zhang, Xixi","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","last_name":"Zhang"},{"full_name":"Xu, Chongyi","last_name":"Xu","first_name":"Chongyi"},{"last_name":"Hu","full_name":"Hu, Yuxin","first_name":"Yuxin"}],"isi":1,"intvolume":"         7","volume":7,"pmid":1,"date_published":"2021-11-11T00:00:00Z","article_processing_charge":"No","page":"1495–1504 ","publisher":"Springer Nature","ddc":["580"],"year":"2021","OA_place":"repository","article_type":"original","OA_type":"green","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Catabolism of strigolactones by a carboxylesterase","has_accepted_license":"1","oa":1,"abstract":[{"text":"Strigolactones (SLs) are carotenoid-derived plant hormones that control shoot branching and communications between host plants and symbiotic fungi or root parasitic plants. Extensive studies have identified the key components participating in SL biosynthesis and signalling, whereas the catabolism or deactivation of endogenous SLs in planta remains largely unknown. Here, we report that the Arabidopsis carboxylesterase 15 (AtCXE15) and its orthologues function as efficient hydrolases of SLs. We show that overexpression of AtCXE15 promotes shoot branching by dampening SL-inhibited axillary bud outgrowth. We further demonstrate that AtCXE15 could bind and efficiently hydrolyse SLs both in vitro and in planta. We also provide evidence that AtCXE15 is capable of catalysing hydrolysis of diverse SL analogues and that such CXE15-dependent catabolism of SLs is evolutionarily conserved in seed plants. These results disclose a catalytic mechanism underlying homoeostatic regulation of SLs in plants, which also provides a rational approach to spatial-temporally manipulate the endogenous SLs and thus architecture of crops and ornamental plants.","lang":"eng"}],"publication_identifier":{"eissn":["2055-0278"]},"date_created":"2021-11-21T23:01:30Z","publication":"Nature Plants","file_date_updated":"2025-01-21T12:41:43Z","date_updated":"2025-01-21T12:42:52Z","type":"journal_article","month":"11","day":"11","doi":"10.1038/s41477-021-01011-y","publication_status":"published","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"quality_controlled":"1","oa_version":"Submitted Version","status":"public","file":[{"relation":"main_file","checksum":"d20231806bea67f0fd19e96a94a048f4","content_type":"application/pdf","success":1,"access_level":"open_access","file_size":41109943,"creator":"dernst","date_updated":"2025-01-21T12:41:43Z","file_name":"Accepted version_Xu et al.,2021 Catabolism of strigolactones by a carboxylesterase.pdf","date_created":"2025-01-21T12:41:43Z","file_id":"18864"}],"citation":{"ista":"Xu E, Chai L, Zhang S, Yu R, Zhang X, Xu C, Hu Y. 2021. Catabolism of strigolactones by a carboxylesterase. Nature Plants. 7, 1495–1504.","mla":"Xu, Enjun, et al. “Catabolism of Strigolactones by a Carboxylesterase.” <i>Nature Plants</i>, vol. 7, Springer Nature, 2021, pp. 1495–1504, doi:<a href=\"https://doi.org/10.1038/s41477-021-01011-y\">10.1038/s41477-021-01011-y</a>.","chicago":"Xu, Enjun, Liang Chai, Shiqi Zhang, Ruixue Yu, Xixi Zhang, Chongyi Xu, and Yuxin Hu. “Catabolism of Strigolactones by a Carboxylesterase.” <i>Nature Plants</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41477-021-01011-y\">https://doi.org/10.1038/s41477-021-01011-y</a>.","short":"E. Xu, L. Chai, S. Zhang, R. Yu, X. Zhang, C. Xu, Y. Hu, Nature Plants 7 (2021) 1495–1504.","ama":"Xu E, Chai L, Zhang S, et al. Catabolism of strigolactones by a carboxylesterase. <i>Nature Plants</i>. 2021;7:1495–1504. doi:<a href=\"https://doi.org/10.1038/s41477-021-01011-y\">10.1038/s41477-021-01011-y</a>","apa":"Xu, E., Chai, L., Zhang, S., Yu, R., Zhang, X., Xu, C., &#38; Hu, Y. (2021). Catabolism of strigolactones by a carboxylesterase. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-021-01011-y\">https://doi.org/10.1038/s41477-021-01011-y</a>","ieee":"E. Xu <i>et al.</i>, “Catabolism of strigolactones by a carboxylesterase,” <i>Nature Plants</i>, vol. 7. Springer Nature, pp. 1495–1504, 2021."},"scopus_import":"1","acknowledgement":"We thank J. Li (Institute of Genetics and Developmental Biology, China) for providing the at14-1, atmax2-1, atmax3-9, atmax4-1, atmax1-1, kai2-2 (Col-0 background) mutants and B. Xu for providing the complementary DNA of P. patens. We are grateful to L. Wang for assistance with MST, B. Han for assistance with UPLC–MS, J. Li for assistance with confocal microscopy and B. Mikael and J. Zhang for their comments on the manuscript. This work was supported by grants from Strategic Priority Research Program of Chinese Academy of Sciences (Y.H., XDB27030102) and the National Natural Science Foundation of China (E.X., 31700253; Y.H., 31830055)."},{"year":"2021","publisher":"Wiley","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985"},{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425"}],"ddc":["580"],"article_processing_charge":"Yes (via OA deal)","page":"351-369","pmid":1,"date_published":"2021-01-01T00:00:00Z","volume":229,"intvolume":"       229","isi":1,"author":[{"full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","last_name":"Li","orcid":"0000-0001-5039-9660","first_name":"Hongjiang"},{"last_name":"von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87","full_name":"von Wangenheim, Daniel","first_name":"Daniel","orcid":"0000-0002-6862-1247"},{"last_name":"Zhang","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","full_name":"Zhang, Xixi","first_name":"Xixi","orcid":"0000-0001-7048-4627"},{"orcid":"0000-0002-0471-8285","first_name":"Shutang","full_name":"Tan, Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan"},{"first_name":"Nasser","orcid":"0000-0002-8821-8236","last_name":"Darwish-Miranda","id":"39CD9926-F248-11E8-B48F-1D18A9856A87","full_name":"Darwish-Miranda, Nasser"},{"full_name":"Naramoto, Satoshi","last_name":"Naramoto","first_name":"Satoshi"},{"full_name":"Wabnik, Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","last_name":"Wabnik","orcid":"0000-0001-7263-0560","first_name":"Krzysztof T"},{"first_name":"Riet","last_name":"de Rycke","full_name":"de Rycke, Riet"},{"orcid":"0000-0001-9735-5315","first_name":"Walter","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann"},{"last_name":"Gütl","full_name":"Gütl, Daniel J","id":"381929CE-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel J"},{"last_name":"Tejos","full_name":"Tejos, Ricardo","first_name":"Ricardo"},{"full_name":"Grones, Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87","last_name":"Grones","first_name":"Peter"},{"first_name":"Meiyu","full_name":"Ke, Meiyu","last_name":"Ke"},{"first_name":"Xu","full_name":"Chen, Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","last_name":"Chen"},{"first_name":"Jan","last_name":"Dettmer","full_name":"Dettmer, Jan"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"issue":"1","_id":"8582","external_id":{"isi":["000570187900001"],"pmid":["32810889"]},"acknowledgement":"We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg) for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1 mutant and the complementation lines, and Dr Patricia C. Zambryski (University of California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria) for assistance with imaging, group members for discussions, Martine De Cock for help in preparing the manuscript and Nataliia Gnyliukh for critical reading and revision of the manuscript. This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 742985) and Comisión Nacional de Investigación Científica y Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007‐2013) under REA grant agreement no. 291734.","scopus_import":"1","status":"public","citation":{"apa":"Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto, S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>","ama":"Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;229(1):351-369. doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>","ieee":"H. Li <i>et al.</i>, “Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 229, no. 1. Wiley, pp. 351–369, 2021.","ista":"Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. New Phytologist. 229(1), 351–369.","short":"H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto, K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke, X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.","mla":"Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 229, no. 1, Wiley, 2021, pp. 351–69, doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>.","chicago":"Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>."},"file":[{"date_updated":"2021-02-04T09:44:17Z","file_size":4061962,"creator":"dernst","date_created":"2021-02-04T09:44:17Z","file_id":"9084","file_name":"2021_NewPhytologist_Li.pdf","content_type":"application/pdf","relation":"main_file","checksum":"b45621607b4cab97eeb1605ab58e896e","success":1,"access_level":"open_access"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"acknowledged_ssus":[{"_id":"Bio"}],"language":[{"iso":"eng"}],"quality_controlled":"1","oa_version":"Published Version","month":"01","ec_funded":1,"doi":"10.1111/nph.16887","day":"01","department":[{"_id":"JiFr"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"EvBe"}],"publication_status":"published","file_date_updated":"2021-02-04T09:44:17Z","date_updated":"2025-06-12T06:32:24Z","type":"journal_article","abstract":[{"lang":"eng","text":"Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear.\r\nHere, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze‐fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall components as well as connections between the cell wall and the plasma membrane.\r\nThis study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems."}],"publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"date_created":"2020-09-28T08:59:28Z","publication":"New Phytologist","has_accepted_license":"1","oa":1,"article_type":"original","title":"Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"oa":1,"has_accepted_license":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"GhARF16-1 modulates leaf development by transcriptionally regulating the GhKNOX2-1 gene in cotton","OA_type":"gold","article_type":"original","OA_place":"publisher","DOAJ_listed":"1","type":"journal_article","date_updated":"2025-07-10T11:57:13Z","file_date_updated":"2021-04-12T12:29:07Z","publication":"Plant Biotechnology Journal","date_created":"2020-10-05T12:44:33Z","publication_identifier":{"issn":["1467-7644"],"eissn":["1467-7652"]},"abstract":[{"text":"The leaf is a crucial organ evolved with remarkable morphological diversity to maximize plant photosynthesis. The leaf shape is a key trait that affects photosynthesis, flowering rates, disease resistance, and yield. Although many genes regulating leaf development have been identified in the past years, the precise regulatory architecture underlying the generation of diverse leaf shapes remains to be elucidated. We used cotton as a reference model to probe the genetic framework underlying divergent leaf forms. Comparative transcriptome analysis revealed that the GhARF16‐1 and GhKNOX2‐1 genes might be potential regulators of leaf shape. We functionally characterized the auxin‐responsive factor ARF16‐1 acting upstream of GhKNOX2‐1 to determine leaf morphology in cotton. The transcription of GhARF16‐1 was significantly higher in lobed‐leaved cotton than in smooth‐leaved cotton. Furthermore, the overexpression of GhARF16‐1 led to the upregulation of GhKNOX2‐1 and resulted in more and deeper serrations in cotton leaves, similar to the leaf shape of cotton plants overexpressing GhKNOX2‐1. We found that GhARF16‐1 specifically bound to the promoter of GhKNOX2‐1 to induce its expression. The heterologous expression of GhARF16‐1 and GhKNOX2‐1 in Arabidopsis led to lobed and curly leaves, and a genetic analysis revealed that GhKNOX2‐1 is epistatic to GhARF16‐1 in Arabidopsis, suggesting that the GhARF16‐1 and GhKNOX2‐1 interaction paradigm also functions to regulate leaf shape in Arabidopsis. To our knowledge, our results uncover a novel mechanism by which auxin, through the key component ARF16‐1 and its downstream‐activated gene KNOX2‐1, determines leaf morphology in eudicots.","lang":"eng"}],"oa_version":"Published Version","language":[{"iso":"eng"}],"quality_controlled":"1","department":[{"_id":"JiFr"}],"publication_status":"published","day":"01","doi":"10.1111/pbi.13484","month":"03","acknowledgement":"We are thankful to Professor Yuxian Zhu from Wuhan University for his extremely valuable remarks and helpful comments on the manuscript. This work was supported by the Shaanxi Natural Science Foundation (2019JQ‐062 and 2020JQ‐410), Shaanxi Youth Entrusted Talents Program (20190205), China Postdoctoral Science Foundation (2018M640947, 2020T130394), Shaanxi Postdoctoral Project (2018BSHYDZZ76), Natural Science Basic Research Plan in Shaanxi Province of China (2018JZ3006), Fundamental Research Funds for the Central Universities (GK201903064, GK201901004, GK202002005 and GK202001004), and State Key Laboratory of Cotton Biology Open Fund (CB2020A12).","scopus_import":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"citation":{"ista":"He P, Zhang Y, Li H, Fu X, Shang H, Zou C, Friml J, Xiao G. 2021. GhARF16-1 modulates leaf development by transcriptionally regulating the GhKNOX2-1 gene in cotton. Plant Biotechnology Journal. 19(3), 548–562.","short":"P. He, Y. Zhang, H. Li, X. Fu, H. Shang, C. Zou, J. Friml, G. Xiao, Plant Biotechnology Journal 19 (2021) 548–562.","mla":"He, P., et al. “GhARF16-1 Modulates Leaf Development by Transcriptionally Regulating the GhKNOX2-1 Gene in Cotton.” <i>Plant Biotechnology Journal</i>, vol. 19, no. 3, Wiley, 2021, pp. 548–62, doi:<a href=\"https://doi.org/10.1111/pbi.13484\">10.1111/pbi.13484</a>.","chicago":"He, P, Yuzhou Zhang, H Li, X Fu, H Shang, C Zou, Jiří Friml, and G Xiao. “GhARF16-1 Modulates Leaf Development by Transcriptionally Regulating the GhKNOX2-1 Gene in Cotton.” <i>Plant Biotechnology Journal</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/pbi.13484\">https://doi.org/10.1111/pbi.13484</a>.","apa":"He, P., Zhang, Y., Li, H., Fu, X., Shang, H., Zou, C., … Xiao, G. (2021). GhARF16-1 modulates leaf development by transcriptionally regulating the GhKNOX2-1 gene in cotton. <i>Plant Biotechnology Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/pbi.13484\">https://doi.org/10.1111/pbi.13484</a>","ama":"He P, Zhang Y, Li H, et al. GhARF16-1 modulates leaf development by transcriptionally regulating the GhKNOX2-1 gene in cotton. <i>Plant Biotechnology Journal</i>. 2021;19(3):548-562. doi:<a href=\"https://doi.org/10.1111/pbi.13484\">10.1111/pbi.13484</a>","ieee":"P. He <i>et al.</i>, “GhARF16-1 modulates leaf development by transcriptionally regulating the GhKNOX2-1 gene in cotton,” <i>Plant Biotechnology Journal</i>, vol. 19, no. 3. Wiley, pp. 548–562, 2021."},"file":[{"file_id":"9321","date_created":"2021-04-12T12:29:07Z","file_name":"2021_PlantBiotechJournal_He.pdf","date_updated":"2021-04-12T12:29:07Z","creator":"dernst","file_size":15691871,"access_level":"open_access","success":1,"content_type":"application/pdf","checksum":"63845be37fb962586e0c7773f2355970","relation":"main_file"}],"status":"public","author":[{"last_name":"He","full_name":"He, P","first_name":"P"},{"full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","last_name":"Zhang","orcid":"0000-0003-2627-6956","first_name":"Yuzhou"},{"first_name":"H","full_name":"Li, H","last_name":"Li"},{"first_name":"X","last_name":"Fu","full_name":"Fu, X"},{"first_name":"H","full_name":"Shang, H","last_name":"Shang"},{"full_name":"Zou, C","last_name":"Zou","first_name":"C"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"},{"full_name":"Xiao, G","last_name":"Xiao","first_name":"G"}],"isi":1,"_id":"8606","external_id":{"isi":["000577682300001"],"pmid":["32981232"]},"issue":"3","volume":19,"intvolume":"        19","page":"548-562","article_processing_charge":"No","date_published":"2021-03-01T00:00:00Z","pmid":1,"year":"2021","ddc":["580"],"publisher":"Wiley"},{"author":[{"first_name":"M","full_name":"Ke, M","last_name":"Ke"},{"first_name":"Z","last_name":"Ma","full_name":"Ma, Z"},{"first_name":"D","full_name":"Wang, D","last_name":"Wang"},{"first_name":"Y","last_name":"Sun","full_name":"Sun, Y"},{"last_name":"Wen","full_name":"Wen, C","first_name":"C"},{"last_name":"Huang","full_name":"Huang, D","first_name":"D"},{"first_name":"Z","last_name":"Chen","full_name":"Chen, Z"},{"first_name":"L","last_name":"Yang","full_name":"Yang, L"},{"first_name":"Shutang","orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","last_name":"Tan"},{"first_name":"R","last_name":"Li","full_name":"Li, R"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"last_name":"Miao","full_name":"Miao, Y","first_name":"Y"},{"first_name":"X","last_name":"Chen","full_name":"Chen, X"}],"isi":1,"issue":"2","_id":"8608","external_id":{"isi":["000573568000001"],"pmid":["32901934"]},"volume":229,"intvolume":"       229","page":"963-978","article_processing_charge":"No","date_published":"2021-01-01T00:00:00Z","pmid":1,"year":"2021","ddc":["580"],"publisher":"Wiley","oa":1,"has_accepted_license":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Salicylic acid regulates PIN2 auxin transporter hyper-clustering and root gravitropic growth via Remorin-dependent lipid nanodomain organization in Arabidopsis thaliana","article_type":"original","type":"journal_article","date_updated":"2023-09-05T16:06:24Z","file_date_updated":"2021-02-04T09:53:16Z","date_created":"2020-10-05T12:45:36Z","publication":"New Phytologist","publication_identifier":{"issn":["0028-646x"],"eissn":["1469-8137"]},"abstract":[{"text":"To adapt to the diverse array of biotic and abiotic cues, plants have evolved sophisticated mechanisms to sense changes in environmental conditions and modulate their growth. Growth-promoting hormones and defence signalling fine tune plant development antagonistically. During host-pathogen interactions, this defence-growth trade-off is mediated by the counteractive effects of the defence hormone salicylic acid (SA) and the growth hormone auxin. Here we revealed an underlying mechanism of SA regulating auxin signalling by constraining the plasma membrane dynamics of PIN2 auxin efflux transporter in Arabidopsis thaliana roots. The lateral diffusion of PIN2 proteins is constrained by SA signalling, during which PIN2 proteins are condensed into hyperclusters depending on REM1.2-mediated nanodomain compartmentalisation. Furthermore, membrane nanodomain compartmentalisation by SA or Remorin (REM) assembly significantly suppressed clathrin-mediated endocytosis. Consequently, SA-induced heterogeneous surface condensation disrupted asymmetric auxin distribution and the resultant gravitropic response. Our results demonstrated a defence-growth trade-off mechanism by which SA signalling crosstalked with auxin transport by concentrating membrane-resident PIN2 into heterogeneous compartments.","lang":"eng"}],"quality_controlled":"1","language":[{"iso":"eng"}],"oa_version":"Published Version","department":[{"_id":"JiFr"}],"publication_status":"published","doi":"10.1111/nph.16915","day":"01","month":"01","scopus_import":"1","acknowledgement":"This work was supported by the National Key Research andDevelopment Programme of China (2017YFA0506100), theNational Natural Science Foundation of China (31870170 and31701168), and the Fok Ying Tung Education Foundation(161027) to XC; NTU startup grant (M4081533) and NIM/01/2016 (NTU, Singapore) to YM. We thank Lei Shi andZhongquan Lin for microscopy assistance.","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"citation":{"ista":"Ke M, Ma Z, Wang D, Sun Y, Wen C, Huang D, Chen Z, Yang L, Tan S, Li R, Friml J, Miao Y, Chen X. 2021. Salicylic acid regulates PIN2 auxin transporter hyper-clustering and root gravitropic growth via Remorin-dependent lipid nanodomain organization in Arabidopsis thaliana. New Phytologist. 229(2), 963–978.","mla":"Ke, M., et al. “Salicylic Acid Regulates PIN2 Auxin Transporter Hyper-Clustering and Root Gravitropic Growth via Remorin-Dependent Lipid Nanodomain Organization in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 229, no. 2, Wiley, 2021, pp. 963–78, doi:<a href=\"https://doi.org/10.1111/nph.16915\">10.1111/nph.16915</a>.","chicago":"Ke, M, Z Ma, D Wang, Y Sun, C Wen, D Huang, Z Chen, et al. “Salicylic Acid Regulates PIN2 Auxin Transporter Hyper-Clustering and Root Gravitropic Growth via Remorin-Dependent Lipid Nanodomain Organization in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.16915\">https://doi.org/10.1111/nph.16915</a>.","short":"M. Ke, Z. Ma, D. Wang, Y. Sun, C. Wen, D. Huang, Z. Chen, L. Yang, S. Tan, R. Li, J. Friml, Y. Miao, X. Chen, New Phytologist 229 (2021) 963–978.","apa":"Ke, M., Ma, Z., Wang, D., Sun, Y., Wen, C., Huang, D., … Chen, X. (2021). Salicylic acid regulates PIN2 auxin transporter hyper-clustering and root gravitropic growth via Remorin-dependent lipid nanodomain organization in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16915\">https://doi.org/10.1111/nph.16915</a>","ama":"Ke M, Ma Z, Wang D, et al. Salicylic acid regulates PIN2 auxin transporter hyper-clustering and root gravitropic growth via Remorin-dependent lipid nanodomain organization in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;229(2):963-978. doi:<a href=\"https://doi.org/10.1111/nph.16915\">10.1111/nph.16915</a>","ieee":"M. Ke <i>et al.</i>, “Salicylic acid regulates PIN2 auxin transporter hyper-clustering and root gravitropic growth via Remorin-dependent lipid nanodomain organization in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 229, no. 2. Wiley, pp. 963–978, 2021."},"file":[{"content_type":"application/pdf","checksum":"d36b6a8c6fafab66264e0d27114dae63","relation":"main_file","access_level":"open_access","success":1,"date_updated":"2021-02-04T09:53:16Z","creator":"dernst","file_size":3674502,"date_created":"2021-02-04T09:53:16Z","file_id":"9085","file_name":"2021_NewPhytologist_Ke.pdf"}],"status":"public"},{"author":[{"first_name":"MM","last_name":"Marquès-Bueno","full_name":"Marquès-Bueno, MM"},{"full_name":"Armengot, L","last_name":"Armengot","first_name":"L"},{"last_name":"Noack","full_name":"Noack, LC","first_name":"LC"},{"first_name":"J","full_name":"Bareille, J","last_name":"Bareille"},{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","full_name":"Rodriguez Solovey, Lesia","last_name":"Rodriguez Solovey","first_name":"Lesia","orcid":"0000-0002-7244-7237"},{"full_name":"Platre, MP","last_name":"Platre","first_name":"MP"},{"first_name":"V","full_name":"Bayle, V","last_name":"Bayle"},{"first_name":"M","last_name":"Liu","full_name":"Liu, M"},{"first_name":"D","last_name":"Opdenacker","full_name":"Opdenacker, D"},{"last_name":"Vanneste","full_name":"Vanneste, S","first_name":"S"},{"full_name":"Möller, BK","last_name":"Möller","first_name":"BK"},{"full_name":"Nimchuk, ZL","last_name":"Nimchuk","first_name":"ZL"},{"first_name":"T","full_name":"Beeckman, T","last_name":"Beeckman"},{"first_name":"AI","last_name":"Caño-Delgado","full_name":"Caño-Delgado, AI"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Y","last_name":"Jaillais","full_name":"Jaillais, Y"}],"isi":1,"external_id":{"isi":["000614361000039"],"pmid":["33157019"]},"_id":"8824","issue":"1","volume":31,"intvolume":"        31","article_processing_charge":"Yes (via OA deal)","date_published":"2021-01-11T00:00:00Z","pmid":1,"year":"2021","publisher":"Elsevier","ddc":["570"],"oa":1,"has_accepted_license":"1","article_type":"original","title":"Auxin-regulated reversible inhibition of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file_date_updated":"2021-02-04T11:37:50Z","type":"journal_article","date_updated":"2024-10-21T06:02:09Z","abstract":[{"lang":"eng","text":"Plants are able to orient their growth according to gravity, which ultimately controls both shoot and root architecture.1 Gravitropism is a dynamic process whereby gravistimulation induces the asymmetric distribution of the plant hormone auxin, leading to asymmetric growth, organ bending, and subsequent reset of auxin distribution back to the original pre-gravistimulation situation.1,  2,  3 Differential auxin accumulation during the gravitropic response depends on the activity of polarly localized PIN-FORMED (PIN) auxin-efflux carriers.1,  2,  3,  4 In particular, the timing of this dynamic response is regulated by PIN2,5,6 but the underlying molecular mechanisms are poorly understood. Here, we show that MEMBRANE ASSOCIATED KINASE REGULATOR2 (MAKR2) controls the pace of the root gravitropic response. We found that MAKR2 is required for the PIN2 asymmetry during gravitropism by acting as a negative regulator of the cell-surface signaling mediated by the receptor-like kinase TRANSMEMBRANE KINASE1 (TMK1).2,7,  8,  9,  10 Furthermore, we show that the MAKR2 inhibitory effect on TMK1 signaling is antagonized by auxin itself, which triggers rapid MAKR2 membrane dissociation in a TMK1-dependent manner. Our findings suggest that the timing of the root gravitropic response is orchestrated by the reversible inhibition of the TMK1 signaling pathway at the cell surface."}],"date_created":"2020-12-01T13:39:46Z","publication":"Current Biology","publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]},"language":[{"iso":"eng"}],"oa_version":"Published Version","quality_controlled":"1","month":"01","publication_status":"published","department":[{"_id":"JiFr"}],"doi":"10.1016/j.cub.2020.10.011","day":"11","scopus_import":"1","acknowledgement":"We thank the SiCE group for discussions and comments; S. Yalovsky, B. Scheres, and the NASC/ABRC collection for providing transgenic Arabidopsis lines and plasmids; L. Kalmbach and M. Barberon for the gift of pLOK180_pFR7m34GW; A. Lacroix, J. Berger, and P. Bolland for plant care; and M. Fendrych for help with microfluidics in the J.F. lab. We acknowledge\r\nthe contribution of the SFR Biosciences (UMS3444/CNRS, US8/Inser m, ENS de Lyon, UCBL) facilities: C. Lionet, E. Chatre, and J. Brocard at LBIPLATIM-MICROSCOPY for assistance with imaging, and V. GuegenChaignon and A. Page at the Protein Science Facility (PSF) for assistance with protein purification and mass spectrometry. Y.J. was funded by ERC\r\ngrant 3363360-APPL under FP/2007–2013. Y.J. and Z.L.N. were funded by an ANR- and NSF-supported ERA-CAPS project (SICOPID: ANR-17-CAPS0003-01/NSF PGRP IOS-1841917). A.I.C.-D. is funded by an ERC consolidator grant (ERC-2015-CoG–683163) and BIO2016-78955 grant from the Spanish Ministry of Economy and Competitiveness. Exchanges between the Y.J. and T.B. laboratories were funded by Tournesol grant 35656NB. B.K.M. was\r\nfunded by the Omics@vib Marie Curie COFUND and Research Foundation Flanders for a postdoctoral fellowship.","status":"public","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"date_updated":"2021-02-04T11:37:50Z","file_size":3458646,"creator":"dernst","file_id":"9090","date_created":"2021-02-04T11:37:50Z","file_name":"2021_CurrentBiology_MarquesBueno.pdf","content_type":"application/pdf","relation":"main_file","checksum":"30b3393d841fb2b1e2b22fb42b5c8fff","success":1,"access_level":"open_access"}],"citation":{"ieee":"M. Marquès-Bueno <i>et al.</i>, “Auxin-regulated reversible inhibition of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism,” <i>Current Biology</i>, vol. 31, no. 1. Elsevier, 2021.","ama":"Marquès-Bueno M, Armengot L, Noack L, et al. Auxin-regulated reversible inhibition of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism. <i>Current Biology</i>. 2021;31(1). doi:<a href=\"https://doi.org/10.1016/j.cub.2020.10.011\">10.1016/j.cub.2020.10.011</a>","apa":"Marquès-Bueno, M., Armengot, L., Noack, L., Bareille, J., Rodriguez Solovey, L., Platre, M., … Jaillais, Y. (2021). Auxin-regulated reversible inhibition of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2020.10.011\">https://doi.org/10.1016/j.cub.2020.10.011</a>","chicago":"Marquès-Bueno, MM, L Armengot, LC Noack, J Bareille, Lesia Rodriguez Solovey, MP Platre, V Bayle, et al. “Auxin-Regulated Reversible Inhibition of TMK1 Signaling by MAKR2 Modulates the Dynamics of Root Gravitropism.” <i>Current Biology</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.cub.2020.10.011\">https://doi.org/10.1016/j.cub.2020.10.011</a>.","mla":"Marquès-Bueno, MM, et al. “Auxin-Regulated Reversible Inhibition of TMK1 Signaling by MAKR2 Modulates the Dynamics of Root Gravitropism.” <i>Current Biology</i>, vol. 31, no. 1, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/j.cub.2020.10.011\">10.1016/j.cub.2020.10.011</a>.","short":"M. Marquès-Bueno, L. Armengot, L. Noack, J. Bareille, L. Rodriguez Solovey, M. Platre, V. Bayle, M. Liu, D. Opdenacker, S. Vanneste, B. Möller, Z. Nimchuk, T. Beeckman, A. Caño-Delgado, J. Friml, Y. Jaillais, Current Biology 31 (2021).","ista":"Marquès-Bueno M, Armengot L, Noack L, Bareille J, Rodriguez Solovey L, Platre M, Bayle V, Liu M, Opdenacker D, Vanneste S, Möller B, Nimchuk Z, Beeckman T, Caño-Delgado A, Friml J, Jaillais Y. 2021. Auxin-regulated reversible inhibition of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism. Current Biology. 31(1)."}},{"date_published":"2021-01-04T00:00:00Z","pmid":1,"page":"151-165","article_processing_charge":"No","ddc":["580"],"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"_id":"256FEF10-B435-11E9-9278-68D0E5697425","name":"Molecular Mechanism underlying Salicylic Acid Regulation of Endocytic Trafficking in Arabidopsis","grant_number":"723-2015"}],"publisher":"Elsevier","year":"2021","issue":"1","_id":"8992","external_id":{"isi":["000605359400014"],"pmid":["33186755"]},"author":[{"orcid":"0000-0002-0471-8285","first_name":"Shutang","last_name":"Tan","full_name":"Tan, Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Christian","last_name":"Luschnig","full_name":"Luschnig, Christian"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"isi":1,"intvolume":"        14","volume":14,"department":[{"_id":"JiFr"}],"publication_status":"published","ec_funded":1,"day":"04","doi":"10.1016/j.molp.2020.11.004","month":"01","oa_version":"Published Version","quality_controlled":"1","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"citation":{"mla":"Tan, Shutang, et al. “Pho-View of Auxin: Reversible Protein Phosphorylation in Auxin Biosynthesis, Transport and Signaling.” <i>Molecular Plant</i>, vol. 14, no. 1, Elsevier, 2021, pp. 151–65, doi:<a href=\"https://doi.org/10.1016/j.molp.2020.11.004\">10.1016/j.molp.2020.11.004</a>.","chicago":"Tan, Shutang, Christian Luschnig, and Jiří Friml. “Pho-View of Auxin: Reversible Protein Phosphorylation in Auxin Biosynthesis, Transport and Signaling.” <i>Molecular Plant</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.molp.2020.11.004\">https://doi.org/10.1016/j.molp.2020.11.004</a>.","short":"S. Tan, C. Luschnig, J. Friml, Molecular Plant 14 (2021) 151–165.","ista":"Tan S, Luschnig C, Friml J. 2021. Pho-view of auxin: Reversible protein phosphorylation in auxin biosynthesis, transport and signaling. Molecular Plant. 14(1), 151–165.","ieee":"S. Tan, C. Luschnig, and J. Friml, “Pho-view of auxin: Reversible protein phosphorylation in auxin biosynthesis, transport and signaling,” <i>Molecular Plant</i>, vol. 14, no. 1. Elsevier, pp. 151–165, 2021.","apa":"Tan, S., Luschnig, C., &#38; Friml, J. (2021). Pho-view of auxin: Reversible protein phosphorylation in auxin biosynthesis, transport and signaling. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2020.11.004\">https://doi.org/10.1016/j.molp.2020.11.004</a>","ama":"Tan S, Luschnig C, Friml J. Pho-view of auxin: Reversible protein phosphorylation in auxin biosynthesis, transport and signaling. <i>Molecular Plant</i>. 2021;14(1):151-165. doi:<a href=\"https://doi.org/10.1016/j.molp.2020.11.004\">10.1016/j.molp.2020.11.004</a>"},"file":[{"date_updated":"2021-01-07T14:03:53Z","file_size":871088,"creator":"dernst","date_created":"2021-01-07T14:03:53Z","file_id":"8995","file_name":"2020_MolecularPlant_Tan.pdf","content_type":"application/pdf","relation":"main_file","checksum":"917e60e57092f22e16beac70b1775ea6","success":1,"access_level":"open_access"}],"status":"public","scopus_import":"1","acknowledgement":"This work was supported by the European Union’s Horizon 2020 Program (ERC grant agreement no. 742985 to J.F.). S.T. was funded by a European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). C.L. is supported by the Austrian Science Fund (FWF; P 31493).","title":"Pho-view of auxin: Reversible protein phosphorylation in auxin biosynthesis, transport and signaling","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","has_accepted_license":"1","oa":1,"publication":"Molecular Plant","date_created":"2021-01-03T23:01:23Z","publication_identifier":{"issn":["1674-2052"],"eissn":["1752-9867"]},"abstract":[{"text":"The phytohormone auxin plays a central role in shaping plant growth and development. With decades of genetic and biochemical studies, numerous core molecular components and their networks, underlying auxin biosynthesis, transport, and signaling, have been identified. Notably, protein phosphorylation, catalyzed by kinases and oppositely hydrolyzed by phosphatases, has been emerging to be a crucial type of post-translational modification, regulating physiological and developmental auxin output at all levels. In this review, we comprehensively discuss earlier and recent advances in our understanding of genetics, biochemistry, and cell biology of the kinases and phosphatases participating in auxin action. We provide insights into the mechanisms by which reversible protein phosphorylation defines developmental auxin responses, discuss current challenges, and provide our perspectives on future directions involving the integration of the control of protein phosphorylation into the molecular auxin network.","lang":"eng"}],"type":"journal_article","date_updated":"2025-07-10T12:01:28Z","file_date_updated":"2021-01-07T14:03:53Z"},{"isi":1,"author":[{"last_name":"Abas","full_name":"Abas, Lindy","first_name":"Lindy"},{"last_name":"Kolb","full_name":"Kolb, Martina","first_name":"Martina"},{"full_name":"Stadlmann, Johannes","last_name":"Stadlmann","first_name":"Johannes"},{"first_name":"Dorina P.","last_name":"Janacek","full_name":"Janacek, Dorina P."},{"first_name":"Kristina","orcid":"0000-0003-1581-881X","last_name":"Lukic","id":"2B04DB84-F248-11E8-B48F-1D18A9856A87","full_name":"Lukic, Kristina"},{"last_name":"Schwechheimer","full_name":"Schwechheimer, Claus","first_name":"Claus"},{"full_name":"Sazanov, Leonid A","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","last_name":"Sazanov","orcid":"0000-0002-0977-7989","first_name":"Leonid A"},{"last_name":"Mach","full_name":"Mach, Lukas","first_name":"Lukas"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml"},{"last_name":"Hammes","full_name":"Hammes, Ulrich Z.","first_name":"Ulrich Z."}],"_id":"8993","external_id":{"isi":["000607270100073"],"pmid":["33443187"]},"issue":"1","volume":118,"intvolume":"       118","article_processing_charge":"No","date_published":"2021-01-05T00:00:00Z","pmid":1,"article_number":"e2020857118","year":"2021","ddc":["580"],"publisher":"National Academy of Sciences","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985"}],"oa":1,"title":"Naphthylphthalamic acid associates with and inhibits PIN auxin transporters","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","date_updated":"2026-06-18T19:38:20Z","type":"journal_article","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"publication":"Proceedings of the National Academy of Sciences of the United States of America","date_created":"2021-01-03T23:01:23Z","abstract":[{"text":"N-1-naphthylphthalamic acid (NPA) is a key inhibitor of directional (polar) transport of the hormone auxin in plants. For decades, it has been a pivotal tool in elucidating the unique polar auxin transport-based processes underlying plant growth and development. Its exact mode of action has long been sought after and is still being debated, with prevailing mechanistic schemes describing only indirect connections between NPA and the main transporters responsible for directional transport, namely PIN auxin exporters. Here we present data supporting a model in which NPA associates with PINs in a more direct manner than hitherto postulated. We show that NPA inhibits PIN activity in a heterologous oocyte system and that expression of NPA-sensitive PINs in plant, yeast, and oocyte membranes leads to specific saturable NPA binding. We thus propose that PINs are a bona fide NPA target. This offers a straightforward molecular basis for NPA inhibition of PIN-dependent auxin transport and a logical parsimonious explanation for the known physiological effects of NPA on plant growth, as well as an alternative hypothesis to interpret past and future results. We also introduce PIN dimerization and describe an effect of NPA on this, suggesting that NPA binding could be exploited to gain insights into structural aspects of PINs related to their transport mechanism.","lang":"eng"}],"oa_version":"Published Version","language":[{"iso":"eng"}],"quality_controlled":"1","ec_funded":1,"doi":"10.1073/pnas.2020857118","day":"05","department":[{"_id":"JiFr"},{"_id":"LeSa"}],"publication_status":"published","month":"01","related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1073/pnas.2102232118"}]},"acknowledgement":"This work was supported by Austrian Science Fund Grant FWF P21533-B20 (to L.A.); German Research Foundation Grant DFG HA3468/6-1 (to U.Z.H.); and European Research Council Grant 742985 (to J.F.). We thank Herta Steinkellner and Alexandra Castilho for N. benthamiana plants, Fabian Nagelreiter for statistical advice, Lanassa Bassukas for help with [ɣ32P]-\r\nATP assays, and Josef Penninger for providing access to mass spectrometry instruments at the Vienna BioCenter Core Facilities. We thank PNAS reviewers for the many comments and suggestions that helped to improve this manuscript.","scopus_import":"1","citation":{"ista":"Abas L, Kolb M, Stadlmann J, Janacek DP, Lukic K, Schwechheimer C, Sazanov LA, Mach L, Friml J, Hammes UZ. 2021. Naphthylphthalamic acid associates with and inhibits PIN auxin transporters. Proceedings of the National Academy of Sciences of the United States of America. 118(1), e2020857118.","short":"L. Abas, M. Kolb, J. Stadlmann, D.P. Janacek, K. Lukic, C. Schwechheimer, L.A. Sazanov, L. Mach, J. Friml, U.Z. Hammes, Proceedings of the National Academy of Sciences of the United States of America 118 (2021).","chicago":"Abas, Lindy, Martina Kolb, Johannes Stadlmann, Dorina P. Janacek, Kristina Lukic, Claus Schwechheimer, Leonid A Sazanov, Lukas Mach, Jiří Friml, and Ulrich Z. Hammes. “Naphthylphthalamic Acid Associates with and Inhibits PIN Auxin Transporters.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2021. <a href=\"https://doi.org/10.1073/pnas.2020857118\">https://doi.org/10.1073/pnas.2020857118</a>.","mla":"Abas, Lindy, et al. “Naphthylphthalamic Acid Associates with and Inhibits PIN Auxin Transporters.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 1, e2020857118, National Academy of Sciences, 2021, doi:<a href=\"https://doi.org/10.1073/pnas.2020857118\">10.1073/pnas.2020857118</a>.","ama":"Abas L, Kolb M, Stadlmann J, et al. Naphthylphthalamic acid associates with and inhibits PIN auxin transporters. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2021;118(1). doi:<a href=\"https://doi.org/10.1073/pnas.2020857118\">10.1073/pnas.2020857118</a>","apa":"Abas, L., Kolb, M., Stadlmann, J., Janacek, D. P., Lukic, K., Schwechheimer, C., … Hammes, U. Z. (2021). Naphthylphthalamic acid associates with and inhibits PIN auxin transporters. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2020857118\">https://doi.org/10.1073/pnas.2020857118</a>","ieee":"L. Abas <i>et al.</i>, “Naphthylphthalamic acid associates with and inhibits PIN auxin transporters,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 1. National Academy of Sciences, 2021."},"main_file_link":[{"url":"https://doi.org/10.1073/pnas.2020857118","open_access":"1"}],"status":"public"},{"status":"public","file":[{"file_name":"Zhao PlantCellEnv 2021_accepted.pdf","file_id":"14481","date_created":"2023-11-02T17:02:11Z","file_size":8437528,"creator":"amally","date_updated":"2023-11-02T17:02:11Z","success":1,"access_level":"open_access","relation":"main_file","checksum":"a812418fede076741c9c4dc07f317068","content_type":"application/pdf"}],"citation":{"ista":"Zhao Y, Wu L, Fu Q, Wang D, Li J, Yao B, Yu S, Jiang L, Qian J, Zhou X, Han L, Zhao S, Ma C, Zhang Y, Luo C, Dong Q, Li S, Zhang L, Jiang X, Li Y, Luo H, Li K, Yang J, Luo Q, Li L, Peng S, Huang H, Zuo Z, Liu C, Wang L, Li C, He X, Friml J, Du Y. 2021. INDITTO2 transposon conveys auxin-mediated DRO1 transcription for rice drought avoidance. Plant, Cell &#38; Environment. 44(6), 1846–1857.","chicago":"Zhao, Y, L Wu, Q Fu, D Wang, J Li, B Yao, S Yu, et al. “INDITTO2 Transposon Conveys Auxin-Mediated DRO1 Transcription for Rice Drought Avoidance.” <i>Plant, Cell &#38; Environment</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/pce.14029\">https://doi.org/10.1111/pce.14029</a>.","short":"Y. Zhao, L. Wu, Q. Fu, D. Wang, J. Li, B. Yao, S. Yu, L. Jiang, J. Qian, X. Zhou, L. Han, S. Zhao, C. Ma, Y. Zhang, C. Luo, Q. Dong, S. Li, L. Zhang, X. Jiang, Y. Li, H. Luo, K. Li, J. Yang, Q. Luo, L. Li, S. Peng, H. Huang, Z. Zuo, C. Liu, L. Wang, C. Li, X. He, J. Friml, Y. Du, Plant, Cell &#38; Environment 44 (2021) 1846–1857.","mla":"Zhao, Y., et al. “INDITTO2 Transposon Conveys Auxin-Mediated DRO1 Transcription for Rice Drought Avoidance.” <i>Plant, Cell &#38; Environment</i>, vol. 44, no. 6, Wiley, 2021, pp. 1846–57, doi:<a href=\"https://doi.org/10.1111/pce.14029\">10.1111/pce.14029</a>.","apa":"Zhao, Y., Wu, L., Fu, Q., Wang, D., Li, J., Yao, B., … Du, Y. (2021). INDITTO2 transposon conveys auxin-mediated DRO1 transcription for rice drought avoidance. <i>Plant, Cell &#38; Environment</i>. Wiley. <a href=\"https://doi.org/10.1111/pce.14029\">https://doi.org/10.1111/pce.14029</a>","ama":"Zhao Y, Wu L, Fu Q, et al. INDITTO2 transposon conveys auxin-mediated DRO1 transcription for rice drought avoidance. <i>Plant, Cell &#38; Environment</i>. 2021;44(6):1846-1857. doi:<a href=\"https://doi.org/10.1111/pce.14029\">10.1111/pce.14029</a>","ieee":"Y. Zhao <i>et al.</i>, “INDITTO2 transposon conveys auxin-mediated DRO1 transcription for rice drought avoidance,” <i>Plant, Cell &#38; Environment</i>, vol. 44, no. 6. Wiley, pp. 1846–1857, 2021."},"scopus_import":"1","month":"06","publication_status":"published","department":[{"_id":"JiFr"}],"day":"01","doi":"10.1111/pce.14029","language":[{"iso":"eng"}],"quality_controlled":"1","oa_version":"Submitted Version","abstract":[{"lang":"eng","text":"Transposable elements exist widely throughout plant genomes and play important roles in plant evolution. Auxin is an important regulator that is traditionally associated with root development and drought stress adaptation. The DEEPER ROOTING 1 (DRO1) gene is a key component of rice drought avoidance. Here, we identified a transposon that acts as an autonomous auxin‐responsive promoter and its presence at specific genome positions conveys physiological adaptations related to drought avoidance. Rice varieties with high and auxin‐mediated transcription of DRO1 in the root tip show deeper and longer root phenotypes and are thus better adapted to drought. The INDITTO2 transposon contains an auxin response element and displays auxin‐responsive promoter activity; it is thus able to convey auxin regulation of transcription to genes in its proximity. In the rice Acuce, which displays DRO1‐mediated drought adaptation, the INDITTO2 transposon was found to be inserted at the promoter region of the DRO1 locus. Transgenesis‐based insertion of the INDITTO2 transposon into the DRO1 promoter of the non‐adapted rice variety Nipponbare was sufficient to promote its drought avoidance. Our data identify an example of how transposons can act as promoters and convey hormonal regulation to nearby loci, improving plant fitness in response to different abiotic stresses."}],"publication":"Plant, Cell & Environment","date_created":"2021-02-24T10:07:21Z","publication_identifier":{"eissn":["1365-3040"],"issn":["0140-7791"]},"file_date_updated":"2023-11-02T17:02:11Z","type":"journal_article","date_updated":"2023-11-07T08:18:36Z","article_type":"original","title":"INDITTO2 transposon conveys auxin-mediated DRO1 transcription for rice drought avoidance","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","oa":1,"publisher":"Wiley","ddc":["580"],"year":"2021","date_published":"2021-06-01T00:00:00Z","pmid":1,"article_processing_charge":"No","page":"1846-1857","intvolume":"        44","volume":44,"external_id":{"isi":["000625398600001"],"pmid":["33576018"]},"_id":"9189","issue":"6","author":[{"first_name":"Y","full_name":"Zhao, Y","last_name":"Zhao"},{"first_name":"L","last_name":"Wu","full_name":"Wu, L"},{"first_name":"Q","full_name":"Fu, Q","last_name":"Fu"},{"first_name":"D","full_name":"Wang, D","last_name":"Wang"},{"full_name":"Li, J","last_name":"Li","first_name":"J"},{"first_name":"B","full_name":"Yao, B","last_name":"Yao"},{"last_name":"Yu","full_name":"Yu, S","first_name":"S"},{"last_name":"Jiang","full_name":"Jiang, L","first_name":"L"},{"last_name":"Qian","full_name":"Qian, J","first_name":"J"},{"first_name":"X","full_name":"Zhou, X","last_name":"Zhou"},{"full_name":"Han, L","last_name":"Han","first_name":"L"},{"full_name":"Zhao, S","last_name":"Zhao","first_name":"S"},{"first_name":"C","full_name":"Ma, C","last_name":"Ma"},{"full_name":"Zhang, Y","last_name":"Zhang","first_name":"Y"},{"last_name":"Luo","full_name":"Luo, C","first_name":"C"},{"first_name":"Q","full_name":"Dong, Q","last_name":"Dong"},{"last_name":"Li","full_name":"Li, S","first_name":"S"},{"full_name":"Zhang, L","last_name":"Zhang","first_name":"L"},{"last_name":"Jiang","full_name":"Jiang, X","first_name":"X"},{"first_name":"Y","last_name":"Li","full_name":"Li, Y"},{"first_name":"H","last_name":"Luo","full_name":"Luo, H"},{"full_name":"Li, K","last_name":"Li","first_name":"K"},{"last_name":"Yang","full_name":"Yang, J","first_name":"J"},{"first_name":"Q","last_name":"Luo","full_name":"Luo, Q"},{"first_name":"L","last_name":"Li","full_name":"Li, L"},{"full_name":"Peng, S","last_name":"Peng","first_name":"S"},{"first_name":"H","full_name":"Huang, H","last_name":"Huang"},{"full_name":"Zuo, Z","last_name":"Zuo","first_name":"Z"},{"first_name":"C","last_name":"Liu","full_name":"Liu, C"},{"full_name":"Wang, L","last_name":"Wang","first_name":"L"},{"first_name":"C","last_name":"Li","full_name":"Li, C"},{"first_name":"X","last_name":"He","full_name":"He, X"},{"last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"last_name":"Du","full_name":"Du, Y","first_name":"Y"}],"isi":1}]