---
_id: '8721'
abstract:
- lang: eng
text: Spontaneously arising channels that transport the phytohormone auxin provide
positional cues for self-organizing aspects of plant development such as flexible
vasculature regeneration or its patterning during leaf venation. The auxin canalization
hypothesis proposes a feedback between auxin signaling and transport as the underlying
mechanism, but molecular players await discovery. We identified part of the machinery
that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related
Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like
kinase) interact with and phosphorylate PIN auxin transporters. camel and canar
mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization,
which macroscopically manifests as defects in leaf venation and vasculature regeneration
after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback
that coordinates polarization of individual cells during auxin canalization.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: 'We acknowledge M. Glanc and Y. Zhang for providing entryclones;
Vienna Biocenter Core Facilities (VBCF) for recombinantprotein production and purification;
Vienna Biocenter Massspectrometry Facility, Bioimaging, and Life Science Facilities
at IST Austria and Proteomics Core Facility CEITEC for a great assistance.Funding:This
project received funding from the European Research Council (ERC) under the European
Union’s Horizon 2020 research and innovation program (grant agreement 742985) and
Austrian Science Fund (FWF): I 3630-B25 to J.F.and by grants from the Austrian Academy
of Science through the Gregor Mendel Institute (Y.B.) and the Austrian Agency for
International Cooperation in Education and Research (D.D.); the Netherlands Organization
for Scientific Research (NWO; VIDI-864.13.001) (W.S.); the Research Foundation–Flanders
(FWO;Odysseus II G0D0515N) and a European Research Council grant (ERC; StG TORPEDO;
714055) to B.D.R., B.Y., and E.M.; and the Hertha Firnberg Programme postdoctoral
fellowship (T-947) from the FWF Austrian Science Fund to E.S.-L.; J.H. is the recipient
of a DOC Fellowship of the Austrian Academy of Sciences at IST Austria.'
article_processing_charge: No
article_type: original
author:
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Tomas
full_name: Prat, Tomas
id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
last_name: Prat
- first_name: N
full_name: Rydza, N
last_name: Rydza
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: David
full_name: Domjan, David
id: C684CD7A-257E-11EA-9B6F-D8588B4F947F
last_name: Domjan
orcid: 0000-0003-2267-106X
- first_name: E
full_name: Mazur, E
last_name: Mazur
- first_name: E
full_name: Smakowska-Luzan, E
last_name: Smakowska-Luzan
- first_name: W
full_name: Smet, W
last_name: Smet
- first_name: E
full_name: Mor, E
last_name: Mor
- first_name: J
full_name: Nolf, J
last_name: Nolf
- first_name: B
full_name: Yang, B
last_name: Yang
- first_name: W
full_name: Grunewald, W
last_name: Grunewald
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Y
full_name: Belkhadir, Y
last_name: Belkhadir
- first_name: B
full_name: De Rybel, B
last_name: De Rybel
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hajny J, Prat T, Rydza N, et al. Receptor kinase module targets PIN-dependent
auxin transport during canalization. Science. 2020;370(6516):550-557. doi:10.1126/science.aba3178
apa: Hajny, J., Prat, T., Rydza, N., Rodriguez Solovey, L., Tan, S., Verstraeten,
I., … Friml, J. (2020). Receptor kinase module targets PIN-dependent auxin transport
during canalization. Science. American Association for the Advancement
of Science. https://doi.org/10.1126/science.aba3178
chicago: Hajny, Jakub, Tomas Prat, N Rydza, Lesia Rodriguez Solovey, Shutang Tan,
Inge Verstraeten, David Domjan, et al. “Receptor Kinase Module Targets PIN-Dependent
Auxin Transport during Canalization.” Science. American Association for
the Advancement of Science, 2020. https://doi.org/10.1126/science.aba3178.
ieee: J. Hajny et al., “Receptor kinase module targets PIN-dependent auxin
transport during canalization,” Science, vol. 370, no. 6516. American Association
for the Advancement of Science, pp. 550–557, 2020.
ista: Hajny J, Prat T, Rydza N, Rodriguez Solovey L, Tan S, Verstraeten I, Domjan
D, Mazur E, Smakowska-Luzan E, Smet W, Mor E, Nolf J, Yang B, Grunewald W, Molnar
G, Belkhadir Y, De Rybel B, Friml J. 2020. Receptor kinase module targets PIN-dependent
auxin transport during canalization. Science. 370(6516), 550–557.
mla: Hajny, Jakub, et al. “Receptor Kinase Module Targets PIN-Dependent Auxin Transport
during Canalization.” Science, vol. 370, no. 6516, American Association
for the Advancement of Science, 2020, pp. 550–57, doi:10.1126/science.aba3178.
short: J. Hajny, T. Prat, N. Rydza, L. Rodriguez Solovey, S. Tan, I. Verstraeten,
D. Domjan, E. Mazur, E. Smakowska-Luzan, W. Smet, E. Mor, J. Nolf, B. Yang, W.
Grunewald, G. Molnar, Y. Belkhadir, B. De Rybel, J. Friml, Science 370 (2020)
550–557.
date_created: 2020-11-02T10:04:46Z
date_published: 2020-10-30T00:00:00Z
date_updated: 2026-06-18T19:35:33Z
day: '30'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1126/science.aba3178
ec_funded: 1
external_id:
isi:
- '000583031800041'
pmid:
- '33122378'
intvolume: ' 370'
isi: 1
issue: '6516'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://europepmc.org/article/MED/33122378#free-full-text
month: '10'
oa: 1
oa_version: Published Version
page: 550-557
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2699E3D2-B435-11E9-9278-68D0E5697425
grant_number: '25239'
name: Cell surface receptor complexes for PIN polarity and auxin-mediated development
publication: Science
publication_identifier:
eissn:
- 1095-9203
issn:
- 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/molecular-compass-for-cell-orientation/
scopus_import: '1'
status: public
title: Receptor kinase module targets PIN-dependent auxin transport during canalization
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 370
year: '2020'
...
---
_id: '8943'
abstract:
- lang: eng
text: The widely used non-steroidal anti-inflammatory drugs (NSAIDs) are derivatives
of the phytohormone salicylic acid (SA). SA is well known to regulate plant immunity
and development, whereas there have been few reports focusing on the effects of
NSAIDs in plants. Our studies here reveal that NSAIDs exhibit largely overlapping
physiological activities to SA in the model plant Arabidopsis. NSAID treatments
lead to shorter and agravitropic primary roots and inhibited lateral root organogenesis.
Notably, in addition to the SA-like action, which in roots involves binding to
the protein phosphatase 2A (PP2A), NSAIDs also exhibit PP2A-independent effects.
Cell biological and biochemical analyses reveal that many NSAIDs bind directly
to and inhibit the chaperone activity of TWISTED DWARF1, thereby regulating actin
cytoskeleton dynamics and subsequent endosomal trafficking. Our findings uncover
an unexpected bioactivity of human pharmaceuticals in plants and provide insights
into the molecular mechanism underlying the cellular action of this class of anti-inflammatory
compounds.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: "We thank Drs. Sebastian Bednarek (University of Wisconsin-Madison),
Niko Geldner (University of Lausanne), and Karin Schumacher (Heidelberg University)
for kindly sharing published Arabidopsis lines; Dr. Satoshi Naramoto for the pPIN2::PIN2-GFP;
pVHA-a1::VHA-a1-mRFP reporter; the staff at the Life Science Facility and Bioimaging
Facility, Monika Hrtyan, and Dorota Jaworska at IST Austria for technical support;
and Drs. Su Tang (Texas A&M University),\r\nMelinda Abas (BOKU), Eva Benkova´ (IST
Austria), Christian Luschnig (BOKU), Bartel Vanholme (Gent University), and the
Friml group for valuable discussions. The research leading to these findings was
funded by the European Union’s Horizon 2020 program (ERC grant agreement no. 742985,
to J.F.), the People Programme (Marie Curie Actions) of the European Union’s Seventh
Framework Programme (FP7/2007-2013) under REA grant agreement no.\r\n291734, the
Swiss National Funds (31003A_165877, to M.G.), the Ministry of Education, Youth,
and Sports of the Czech Republic (project no. CZ.02.1.01/0.0/0.0/16_019/0000738,
EU Operational Programme ‘‘Research, development and education and Centre for Plant
Experimental Biology’’), and the EU Operational Programme Prague - Competitiveness
(project no. CZ.2.16/3.1.00/21519). S.T. was funded by a European Molecular Biology
Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). X.Z. was
partly supported by a PhD scholarship from the China Scholarship Council."
article_number: '108463'
article_processing_charge: Yes
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Martin
full_name: Di Donato, Martin
last_name: Di Donato
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Petr
full_name: Klíma, Petr
last_name: Klíma
- first_name: Jie
full_name: Liu, Jie
last_name: Liu
- first_name: Aurélien
full_name: Bailly, Aurélien
last_name: Bailly
- first_name: Noel
full_name: Ferro, Noel
last_name: Ferro
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tan S, Di Donato M, Glanc M, et al. Non-steroidal anti-inflammatory drugs target
TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development.
Cell Reports. 2020;33(9). doi:10.1016/j.celrep.2020.108463
apa: Tan, S., Di Donato, M., Glanc, M., Zhang, X., Klíma, P., Liu, J., … Friml,
J. (2020). Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated
actin dynamics and auxin transport-mediated plant development. Cell Reports.
Elsevier. https://doi.org/10.1016/j.celrep.2020.108463
chicago: Tan, Shutang, Martin Di Donato, Matous Glanc, Xixi Zhang, Petr Klíma, Jie
Liu, Aurélien Bailly, et al. “Non-Steroidal Anti-Inflammatory Drugs Target TWISTED
DWARF1-Regulated Actin Dynamics and Auxin Transport-Mediated Plant Development.”
Cell Reports. Elsevier, 2020. https://doi.org/10.1016/j.celrep.2020.108463.
ieee: S. Tan et al., “Non-steroidal anti-inflammatory drugs target TWISTED
DWARF1-regulated actin dynamics and auxin transport-mediated plant development,”
Cell Reports, vol. 33, no. 9. Elsevier, 2020.
ista: Tan S, Di Donato M, Glanc M, Zhang X, Klíma P, Liu J, Bailly A, Ferro N, Petrášek
J, Geisler M, Friml J. 2020. Non-steroidal anti-inflammatory drugs target TWISTED
DWARF1-regulated actin dynamics and auxin transport-mediated plant development.
Cell Reports. 33(9), 108463.
mla: Tan, Shutang, et al. “Non-Steroidal Anti-Inflammatory Drugs Target TWISTED
DWARF1-Regulated Actin Dynamics and Auxin Transport-Mediated Plant Development.”
Cell Reports, vol. 33, no. 9, 108463, Elsevier, 2020, doi:10.1016/j.celrep.2020.108463.
short: S. Tan, M. Di Donato, M. Glanc, X. Zhang, P. Klíma, J. Liu, A. Bailly, N.
Ferro, J. Petrášek, M. Geisler, J. Friml, Cell Reports 33 (2020).
corr_author: '1'
date_created: 2020-12-13T23:01:21Z
date_published: 2020-12-01T00:00:00Z
date_updated: 2026-04-03T09:30:47Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.celrep.2020.108463
ec_funded: 1
external_id:
isi:
- '000595658100018'
pmid:
- '33264621'
file:
- access_level: open_access
checksum: ed18cba0fb48ed2e789381a54cc21904
content_type: application/pdf
creator: dernst
date_created: 2020-12-14T07:33:39Z
date_updated: 2020-12-14T07:33:39Z
file_id: '8948'
file_name: 2020_CellReports_Tan.pdf
file_size: 8056434
relation: main_file
success: 1
file_date_updated: 2020-12-14T07:33:39Z
has_accepted_license: '1'
intvolume: ' 33'
isi: 1
issue: '9'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
grant_number: 723-2015
name: Molecular Mechanism underlying Salicylic Acid Regulation of Endocytic Trafficking
in Arabidopsis
publication: Cell Reports
publication_identifier:
eissn:
- 2211-1247
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/plants-on-aspirin/
scopus_import: '1'
status: public
title: Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin
dynamics and auxin transport-mediated plant development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 33
year: '2020'
...
---
_id: '6997'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Friml J. Auxin guides roots to avoid obstacles during gravitropic
growth. New Phytologist. 2020;225(3):1049-1052. doi:10.1111/nph.16203
apa: Zhang, Y., & Friml, J. (2020). Auxin guides roots to avoid obstacles during
gravitropic growth. New Phytologist. Wiley. https://doi.org/10.1111/nph.16203
chicago: Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during
Gravitropic Growth.” New Phytologist. Wiley, 2020. https://doi.org/10.1111/nph.16203.
ieee: Y. Zhang and J. Friml, “Auxin guides roots to avoid obstacles during gravitropic
growth,” New Phytologist, vol. 225, no. 3. Wiley, pp. 1049–1052, 2020.
ista: Zhang Y, Friml J. 2020. Auxin guides roots to avoid obstacles during gravitropic
growth. New Phytologist. 225(3), 1049–1052.
mla: Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during
Gravitropic Growth.” New Phytologist, vol. 225, no. 3, Wiley, 2020, pp.
1049–52, doi:10.1111/nph.16203.
short: Y. Zhang, J. Friml, New Phytologist 225 (2020) 1049–1052.
corr_author: '1'
date_created: 2019-11-12T11:41:32Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2025-04-14T07:45:04Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.16203
ec_funded: 1
external_id:
isi:
- '000489638800001'
pmid:
- '31603260'
file:
- access_level: open_access
checksum: cd42ffdb381fd52812b9583d4d407139
content_type: application/pdf
creator: dernst
date_created: 2020-11-18T16:42:48Z
date_updated: 2020-11-18T16:42:48Z
file_id: '8772'
file_name: 2020_NewPhytologist_Zhang.pdf
file_size: 717345
relation: main_file
success: 1
file_date_updated: 2020-11-18T16:42:48Z
has_accepted_license: '1'
intvolume: ' 225'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 1049-1052
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin guides roots to avoid obstacles during gravitropic growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 225
year: '2020'
...
---
_id: '7142'
abstract:
- lang: eng
text: The phytohormone auxin acts as an amazingly versatile coordinator of plant
growth and development. With its morphogen-like properties, auxin controls sites
and timing of differentiation and/or growth responses both, in quantitative and
qualitative terms. Specificity in the auxin response depends largely on distinct
modes of signal transmission, by which individual cells perceive and convert auxin
signals into a remarkable diversity of responses. The best understood, or so-called
canonical mechanism of auxin perception ultimately results in variable adjustments
of the cellular transcriptome, via a short, nuclear signal transduction pathway.
Additional findings that accumulated over decades implied that an additional,
presumably, cell surface-based auxin perception mechanism mediates very rapid
cellular responses and decisively contributes to the cell's overall hormonal response.
Recent investigations into both, nuclear and cell surface auxin signalling challenged
this assumed partition of roles for different auxin signalling pathways and revealed
an unexpected complexity in transcriptional and non-transcriptional cellular responses
mediated by auxin.
acknowledgement: Research in J.F. laboratory is funded by the European Union's Horizon
2020 program (ERC grant agreement n° 742985); C.L. is supported by the Austrian
Science Fund (FWF grant P 31493).
article_processing_charge: No
article_type: original
author:
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Gallei MC, Luschnig C, Friml J. Auxin signalling in growth: Schrödinger’s
cat out of the bag. Current Opinion in Plant Biology. 2020;53(2):43-49.
doi:10.1016/j.pbi.2019.10.003'
apa: 'Gallei, M. C., Luschnig, C., & Friml, J. (2020). Auxin signalling in growth:
Schrödinger’s cat out of the bag. Current Opinion in Plant Biology. Elsevier.
https://doi.org/10.1016/j.pbi.2019.10.003'
chicago: 'Gallei, Michelle C, Christian Luschnig, and Jiří Friml. “Auxin Signalling
in Growth: Schrödinger’s Cat out of the Bag.” Current Opinion in Plant Biology.
Elsevier, 2020. https://doi.org/10.1016/j.pbi.2019.10.003.'
ieee: 'M. C. Gallei, C. Luschnig, and J. Friml, “Auxin signalling in growth: Schrödinger’s
cat out of the bag,” Current Opinion in Plant Biology, vol. 53, no. 2.
Elsevier, pp. 43–49, 2020.'
ista: 'Gallei MC, Luschnig C, Friml J. 2020. Auxin signalling in growth: Schrödinger’s
cat out of the bag. Current Opinion in Plant Biology. 53(2), 43–49.'
mla: 'Gallei, Michelle C., et al. “Auxin Signalling in Growth: Schrödinger’s Cat
out of the Bag.” Current Opinion in Plant Biology, vol. 53, no. 2, Elsevier,
2020, pp. 43–49, doi:10.1016/j.pbi.2019.10.003.'
short: M.C. Gallei, C. Luschnig, J. Friml, Current Opinion in Plant Biology 53 (2020)
43–49.
corr_author: '1'
date_created: 2019-12-02T12:05:26Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2026-04-07T14:18:57Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2019.10.003
ec_funded: 1
external_id:
isi:
- '000521120600007'
pmid:
- '31760231'
intvolume: ' 53'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 43-49
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Current Opinion in Plant Biology
publication_identifier:
eissn:
- 1879-0356
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 'Auxin signalling in growth: Schrödinger''s cat out of the bag'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 53
year: '2020'
...
---
_id: '7204'
abstract:
- lang: eng
text: Plant root architecture dynamically adapts to various environmental conditions,
such as salt‐containing soil. The phytohormone abscisic acid (ABA) is involved
among others also in these developmental adaptations, but the underlying molecular
mechanism remains elusive. Here, a novel branch of the ABA signaling pathway in
Arabidopsis involving PYR/PYL/RCAR (abbreviated as PYLs) receptor‐protein phosphatase
2A (PP2A) complex that acts in parallel to the canonical PYLs‐protein phosphatase
2C (PP2C) mechanism is identified. The PYLs‐PP2A signaling modulates root gravitropism
and lateral root formation through regulating phytohormone auxin transport. In
optimal conditions, PYLs ABA receptor interacts with the catalytic subunits of
PP2A, increasing their phosphatase activity and thus counteracting PINOID (PID)
kinase‐mediated phosphorylation of PIN‐FORMED (PIN) auxin transporters. By contrast,
in salt and osmotic stress conditions, ABA binds to PYLs, inhibiting the PP2A
activity, which leads to increased PIN phosphorylation and consequently modulated
directional auxin transport leading to adapted root architecture. This work reveals
an adaptive mechanism that may flexibly adjust plant root growth to withstand
saline and osmotic stresses. It occurs via the cross‐talk between the stress hormone
ABA and the versatile developmental regulator auxin.
article_number: '1901455'
article_processing_charge: No
article_type: original
author:
- first_name: Yang
full_name: Li, Yang
last_name: Li
- first_name: Yaping
full_name: Wang, Yaping
last_name: Wang
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Zhen
full_name: Li, Zhen
last_name: Li
- first_name: Zhi
full_name: Yuan, Zhi
last_name: Yuan
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: David
full_name: Domjan, David
id: C684CD7A-257E-11EA-9B6F-D8588B4F947F
last_name: Domjan
orcid: 0000-0003-2267-106X
- first_name: Kai
full_name: Wang, Kai
last_name: Wang
- first_name: Wei
full_name: Xuan, Wei
last_name: Xuan
- first_name: Yan
full_name: Guo, Yan
last_name: Guo
- first_name: Zhizhong
full_name: Gong, Zhizhong
last_name: Gong
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jing
full_name: Zhang, Jing
last_name: Zhang
citation:
ama: Li Y, Wang Y, Tan S, et al. Root growth adaptation is mediated by PYLs ABA
receptor-PP2A protein phosphatase complex. Advanced Science. 2020;7(3).
doi:10.1002/advs.201901455
apa: Li, Y., Wang, Y., Tan, S., Li, Z., Yuan, Z., Glanc, M., … Zhang, J. (2020).
Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase
complex. Advanced Science. Wiley. https://doi.org/10.1002/advs.201901455
chicago: Li, Yang, Yaping Wang, Shutang Tan, Zhen Li, Zhi Yuan, Matous Glanc, David
Domjan, et al. “Root Growth Adaptation Is Mediated by PYLs ABA Receptor-PP2A Protein
Phosphatase Complex.” Advanced Science. Wiley, 2020. https://doi.org/10.1002/advs.201901455.
ieee: Y. Li et al., “Root growth adaptation is mediated by PYLs ABA receptor-PP2A
protein phosphatase complex,” Advanced Science, vol. 7, no. 3. Wiley, 2020.
ista: Li Y, Wang Y, Tan S, Li Z, Yuan Z, Glanc M, Domjan D, Wang K, Xuan W, Guo
Y, Gong Z, Friml J, Zhang J. 2020. Root growth adaptation is mediated by PYLs
ABA receptor-PP2A protein phosphatase complex. Advanced Science. 7(3), 1901455.
mla: Li, Yang, et al. “Root Growth Adaptation Is Mediated by PYLs ABA Receptor-PP2A
Protein Phosphatase Complex.” Advanced Science, vol. 7, no. 3, 1901455,
Wiley, 2020, doi:10.1002/advs.201901455.
short: Y. Li, Y. Wang, S. Tan, Z. Li, Z. Yuan, M. Glanc, D. Domjan, K. Wang, W.
Xuan, Y. Guo, Z. Gong, J. Friml, J. Zhang, Advanced Science 7 (2020).
date_created: 2019-12-22T23:00:43Z
date_published: 2020-02-05T00:00:00Z
date_updated: 2023-08-17T14:13:17Z
day: '05'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1002/advs.201901455
external_id:
isi:
- '000501912800001'
pmid:
- '32042554'
file:
- access_level: open_access
checksum: 016eeab5860860af038e2da95ffe75c3
content_type: application/pdf
creator: dernst
date_created: 2020-02-24T14:29:54Z
date_updated: 2020-07-14T12:47:53Z
file_id: '7519'
file_name: 2020_AdvScience_Li.pdf
file_size: 3586924
relation: main_file
file_date_updated: 2020-07-14T12:47:53Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Advanced Science
publication_identifier:
eissn:
- 2198-3844
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase
complex
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 7
year: '2020'
...
---
_id: '7219'
abstract:
- lang: eng
text: Root system architecture (RSA), governed by the phytohormone auxin, endows
plants with an adaptive advantage in particular environments. Using geographically
representative arabidopsis (Arabidopsis thaliana) accessions as a resource for
GWA mapping, Waidmann et al. and Ogura et al. recently identified two novel components
involved in modulating auxin-mediated RSA and conferring plant fitness in particular
habitats.
article_processing_charge: No
article_type: original
author:
- first_name: Guanghui
full_name: Xiao, Guanghui
last_name: Xiao
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
citation:
ama: 'Xiao G, Zhang Y. Adaptive growth: Shaping auxin-mediated root system architecture.
Trends in Plant Science. 2020;25(2):P121-123. doi:10.1016/j.tplants.2019.12.001'
apa: 'Xiao, G., & Zhang, Y. (2020). Adaptive growth: Shaping auxin-mediated
root system architecture. Trends in Plant Science. Elsevier. https://doi.org/10.1016/j.tplants.2019.12.001'
chicago: 'Xiao, Guanghui, and Yuzhou Zhang. “Adaptive Growth: Shaping Auxin-Mediated
Root System Architecture.” Trends in Plant Science. Elsevier, 2020. https://doi.org/10.1016/j.tplants.2019.12.001.'
ieee: 'G. Xiao and Y. Zhang, “Adaptive growth: Shaping auxin-mediated root system
architecture,” Trends in Plant Science, vol. 25, no. 2. Elsevier, pp. P121-123,
2020.'
ista: 'Xiao G, Zhang Y. 2020. Adaptive growth: Shaping auxin-mediated root system
architecture. Trends in Plant Science. 25(2), P121-123.'
mla: 'Xiao, Guanghui, and Yuzhou Zhang. “Adaptive Growth: Shaping Auxin-Mediated
Root System Architecture.” Trends in Plant Science, vol. 25, no. 2, Elsevier,
2020, pp. P121-123, doi:10.1016/j.tplants.2019.12.001.'
short: G. Xiao, Y. Zhang, Trends in Plant Science 25 (2020) P121-123.
corr_author: '1'
date_created: 2019-12-29T23:00:48Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2025-07-10T11:54:24Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.tplants.2019.12.001
external_id:
isi:
- '000508637500001'
pmid:
- '31843370'
intvolume: ' 25'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: P121-123
pmid: 1
publication: Trends in Plant Science
publication_identifier:
issn:
- 1360-1385
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Adaptive growth: Shaping auxin-mediated root system architecture'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2020'
...
---
_id: '7416'
abstract:
- lang: eng
text: Earlier, we demonstrated that transcript levels of METAL TOLERANCE PROTEIN2
(MTP2) and of HEAVY METAL ATPase2 (HMA2) increase strongly in roots of Arabidopsis
upon prolonged zinc (Zn) deficiency and respond to shoot physiological Zn status,
and not to the local Zn status in roots. This provided evidence for shoot-to-root
communication in the acclimation of plants to Zn deficiency. Zn-deficient soils
limit both the yield and quality of agricultural crops and can result in clinically
relevant nutritional Zn deficiency in human populations. Implementing Zn deficiency
during cultivation of the model plant Arabidopsis thaliana on agar-solidified
media is difficult because trace element contaminations are present in almost
all commercially available agars. Here, we demonstrate root morphological acclimations
to Zn deficiency on agar-solidified medium following the effective removal of
contaminants. These advancements allow reproducible phenotyping toward understanding
fundamental plant responses to deficiencies of Zn and other essential trace elements.
article_number: '1687175'
article_processing_charge: No
article_type: original
author:
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: U.
full_name: Krämer, U.
last_name: Krämer
citation:
ama: Sinclair SA, Krämer U. Generation of effective zinc-deficient agar-solidified
media allows identification of root morphology changes in response to zinc limitation.
Plant Signaling & Behavior. 2020;15(1). doi:10.1080/15592324.2019.1687175
apa: Sinclair, S. A., & Krämer, U. (2020). Generation of effective zinc-deficient
agar-solidified media allows identification of root morphology changes in response
to zinc limitation. Plant Signaling & Behavior. Taylor & Francis.
https://doi.org/10.1080/15592324.2019.1687175
chicago: Sinclair, Scott A, and U. Krämer. “Generation of Effective Zinc-Deficient
Agar-Solidified Media Allows Identification of Root Morphology Changes in Response
to Zinc Limitation.” Plant Signaling & Behavior. Taylor & Francis,
2020. https://doi.org/10.1080/15592324.2019.1687175.
ieee: S. A. Sinclair and U. Krämer, “Generation of effective zinc-deficient agar-solidified
media allows identification of root morphology changes in response to zinc limitation,”
Plant Signaling & Behavior, vol. 15, no. 1. Taylor & Francis, 2020.
ista: Sinclair SA, Krämer U. 2020. Generation of effective zinc-deficient agar-solidified
media allows identification of root morphology changes in response to zinc limitation.
Plant Signaling & Behavior. 15(1), 1687175.
mla: Sinclair, Scott A., and U. Krämer. “Generation of Effective Zinc-Deficient
Agar-Solidified Media Allows Identification of Root Morphology Changes in Response
to Zinc Limitation.” Plant Signaling & Behavior, vol. 15, no. 1, 1687175,
Taylor & Francis, 2020, doi:10.1080/15592324.2019.1687175.
short: S.A. Sinclair, U. Krämer, Plant Signaling & Behavior 15 (2020).
date_created: 2020-01-30T10:12:04Z
date_published: 2020-01-01T00:00:00Z
date_updated: 2023-10-17T09:01:48Z
day: '01'
department:
- _id: JiFr
doi: 10.1080/15592324.2019.1687175
external_id:
isi:
- '000494909300001'
pmid:
- '31696764'
intvolume: ' 15'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7012054
month: '01'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Plant Signaling & Behavior
publication_identifier:
issn:
- 1559-2324
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
scopus_import: '1'
status: public
title: Generation of effective zinc-deficient agar-solidified media allows identification
of root morphology changes in response to zinc limitation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2020'
...
---
_id: '7417'
abstract:
- lang: eng
text: Previously, we reported that the allelic de-etiolated by zinc (dez) and trichome
birefringence (tbr) mutants exhibit photomorphogenic development in the dark,
which is enhanced by high Zn. TRICHOME BIREFRINGENCE-LIKE proteins had been implicated
in transferring acetyl groups to various hemicelluloses. Pectin O-acetylation
levels were lower in dark-grown dez seedlings than in the wild type. We observed
Zn-enhanced photomorphogenesis in the dark also in the reduced wall acetylation
2 (rwa2-3) mutant, which exhibits lowered O-acetylation levels of cell wall macromolecules
including pectins and xyloglucans, supporting a role for cell wall macromolecule
O-acetylation in the photomorphogenic phenotypes of rwa2-3 and dez. Application
of very short oligogalacturonides (vsOGs) restored skotomorphogenesis in dark-grown
dez and rwa2-3. Here we demonstrate that in dez, O-acetylation of non-pectin cell
wall components, notably of xyloglucan, is enhanced. Our results highlight the
complexity of cell wall homeostasis and indicate against an influence of xyloglucan
O-acetylation on light-dependent seedling development.
article_number: e1687185
article_processing_charge: No
article_type: original
author:
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: S.
full_name: Gille, S.
last_name: Gille
- first_name: M.
full_name: Pauly, M.
last_name: Pauly
- first_name: U.
full_name: Krämer, U.
last_name: Krämer
citation:
ama: Sinclair SA, Gille S, Pauly M, Krämer U. Regulation of acetylation of plant
cell wall components is complex and responds to external stimuli. Plant Signaling
& Behavior. 2020;15(1). doi:10.1080/15592324.2019.1687185
apa: Sinclair, S. A., Gille, S., Pauly, M., & Krämer, U. (2020). Regulation
of acetylation of plant cell wall components is complex and responds to external
stimuli. Plant Signaling & Behavior. Informa UK Limited. https://doi.org/10.1080/15592324.2019.1687185
chicago: Sinclair, Scott A, S. Gille, M. Pauly, and U. Krämer. “Regulation of Acetylation
of Plant Cell Wall Components Is Complex and Responds to External Stimuli.” Plant
Signaling & Behavior. Informa UK Limited, 2020. https://doi.org/10.1080/15592324.2019.1687185.
ieee: S. A. Sinclair, S. Gille, M. Pauly, and U. Krämer, “Regulation of acetylation
of plant cell wall components is complex and responds to external stimuli,” Plant
Signaling & Behavior, vol. 15, no. 1. Informa UK Limited, 2020.
ista: Sinclair SA, Gille S, Pauly M, Krämer U. 2020. Regulation of acetylation of
plant cell wall components is complex and responds to external stimuli. Plant
Signaling & Behavior. 15(1), e1687185.
mla: Sinclair, Scott A., et al. “Regulation of Acetylation of Plant Cell Wall Components
Is Complex and Responds to External Stimuli.” Plant Signaling & Behavior,
vol. 15, no. 1, e1687185, Informa UK Limited, 2020, doi:10.1080/15592324.2019.1687185.
short: S.A. Sinclair, S. Gille, M. Pauly, U. Krämer, Plant Signaling & Behavior
15 (2020).
date_created: 2020-01-30T10:14:14Z
date_published: 2020-01-01T00:00:00Z
date_updated: 2023-09-06T15:23:04Z
day: '01'
department:
- _id: JiFr
doi: 10.1080/15592324.2019.1687185
external_id:
isi:
- '000494907500001'
pmid:
- '31696770'
intvolume: ' 15'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7012154
month: '01'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Plant Signaling & Behavior
publication_identifier:
issn:
- 1559-2324
publication_status: published
publisher: Informa UK Limited
quality_controlled: '1'
scopus_import: '1'
status: public
title: Regulation of acetylation of plant cell wall components is complex and responds
to external stimuli
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 15
year: '2020'
...
---
_id: '7465'
abstract:
- lang: eng
text: The flexible development of plants is characterized by a high capacity for
post-embryonic organ formation and tissue regeneration, processes, which require
tightly regulated intercellular communication and coordinated tissue (re-)polarization.
The phytohormone auxin, the main driver for these processes, is able to establish
polarized auxin transport channels, which are characterized by the expression
and polar, subcellular localization of the PIN1 auxin transport proteins. These
channels are demarcating the position of future vascular strands necessary for
organ formation and tissue regeneration. Major progress has been made in the last
years to understand how PINs can change their polarity in different contexts and
thus guide auxin flow through the plant. However, it still remains elusive how
auxin mediates the establishment of auxin conducting channels and the formation
of vascular tissue and which cellular processes are involved. By the means of
sophisticated regeneration experiments combined with local auxin applications
in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics,
(ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact
actin cytoskeleton required for post-endocytic trafficking are indispensable for
auxin channel formation, de novo vascular formation and vascular regeneration
after wounding. These observations provide novel insights into cellular mechanism
of coordinated tissue polarization during auxin canalization.
article_number: '110414'
article_processing_charge: No
article_type: original
author:
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Hélène S.
full_name: Robert, Hélène S.
last_name: Robert
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. Clathrin-mediated
trafficking and PIN trafficking are required for auxin canalization and vascular
tissue formation in Arabidopsis. Plant Science. 2020;293(4). doi:10.1016/j.plantsci.2020.110414
apa: Mazur, E., Gallei, M. C., Adamowski, M., Han, H., Robert, H. S., & Friml,
J. (2020). Clathrin-mediated trafficking and PIN trafficking are required for
auxin canalization and vascular tissue formation in Arabidopsis. Plant Science.
Elsevier. https://doi.org/10.1016/j.plantsci.2020.110414
chicago: Mazur, Ewa, Michelle C Gallei, Maciek Adamowski, Huibin Han, Hélène S.
Robert, and Jiří Friml. “Clathrin-Mediated Trafficking and PIN Trafficking Are
Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.”
Plant Science. Elsevier, 2020. https://doi.org/10.1016/j.plantsci.2020.110414.
ieee: E. Mazur, M. C. Gallei, M. Adamowski, H. Han, H. S. Robert, and J. Friml,
“Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
and vascular tissue formation in Arabidopsis,” Plant Science, vol. 293,
no. 4. Elsevier, 2020.
ista: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. 2020. Clathrin-mediated
trafficking and PIN trafficking are required for auxin canalization and vascular
tissue formation in Arabidopsis. Plant Science. 293(4), 110414.
mla: Mazur, Ewa, et al. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required
for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” Plant
Science, vol. 293, no. 4, 110414, Elsevier, 2020, doi:10.1016/j.plantsci.2020.110414.
short: E. Mazur, M.C. Gallei, M. Adamowski, H. Han, H.S. Robert, J. Friml, Plant
Science 293 (2020).
corr_author: '1'
date_created: 2020-02-09T23:00:50Z
date_published: 2020-04-01T00:00:00Z
date_updated: 2026-04-07T14:18:57Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.plantsci.2020.110414
ec_funded: 1
external_id:
isi:
- '000520609800009'
pmid:
- '32081263'
file:
- access_level: open_access
checksum: f7f27c6a8fea985ceb9279be2204461c
content_type: application/pdf
creator: dernst
date_created: 2020-02-10T08:59:36Z
date_updated: 2020-07-14T12:47:59Z
file_id: '7471'
file_name: 2020_PlantScience_Mazur.pdf
file_size: 3499069
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 293'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Science
publication_identifier:
eissn:
- 1873-2259
issn:
- 0168-9452
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
and vascular tissue formation in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 293
year: '2020'
...
---
_id: '7490'
abstract:
- lang: eng
text: In plants, clathrin mediated endocytosis (CME) represents the major route
for cargo internalisation from the cell surface. It has been assumed to operate
in an evolutionary conserved manner as in yeast and animals. Here we report characterisation
of ultrastructure, dynamics and mechanisms of plant CME as allowed by our advancement
in electron microscopy and quantitative live imaging techniques. Arabidopsis CME
appears to follow the constant curvature model and the bona fide CME population
generates vesicles of a predominantly hexagonal-basket type; larger and with faster
kinetics than in other models. Contrary to the existing paradigm, actin is dispensable
for CME events at the plasma membrane but plays a unique role in collecting endocytic
vesicles, sorting of internalised cargos and directional endosome movement that
itself actively promote CME events. Internalized vesicles display a strongly delayed
and sequential uncoating. These unique features highlight the independent evolution
of the plant CME mechanism during the autonomous rise of multicellularity in eukaryotes.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
article_number: e52067
article_processing_charge: No
article_type: original
author:
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Roshan
full_name: Prizak, Roshan
id: 4456104E-F248-11E8-B48F-1D18A9856A87
last_name: Prizak
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Barbara E
full_name: Casillas Perez, Barbara E
id: 351ED2AA-F248-11E8-B48F-1D18A9856A87
last_name: Casillas Perez
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Narasimhan M, Johnson AJ, Prizak R, et al. Evolutionarily unique mechanistic
framework of clathrin-mediated endocytosis in plants. eLife. 2020;9. doi:10.7554/eLife.52067
apa: Narasimhan, M., Johnson, A. J., Prizak, R., Kaufmann, W., Tan, S., Casillas
Perez, B. E., & Friml, J. (2020). Evolutionarily unique mechanistic framework
of clathrin-mediated endocytosis in plants. ELife. eLife Sciences Publications.
https://doi.org/10.7554/eLife.52067
chicago: Narasimhan, Madhumitha, Alexander J Johnson, Roshan Prizak, Walter Kaufmann,
Shutang Tan, Barbara E Casillas Perez, and Jiří Friml. “Evolutionarily Unique
Mechanistic Framework of Clathrin-Mediated Endocytosis in Plants.” ELife.
eLife Sciences Publications, 2020. https://doi.org/10.7554/eLife.52067.
ieee: M. Narasimhan et al., “Evolutionarily unique mechanistic framework
of clathrin-mediated endocytosis in plants,” eLife, vol. 9. eLife Sciences
Publications, 2020.
ista: Narasimhan M, Johnson AJ, Prizak R, Kaufmann W, Tan S, Casillas Perez BE,
Friml J. 2020. Evolutionarily unique mechanistic framework of clathrin-mediated
endocytosis in plants. eLife. 9, e52067.
mla: Narasimhan, Madhumitha, et al. “Evolutionarily Unique Mechanistic Framework
of Clathrin-Mediated Endocytosis in Plants.” ELife, vol. 9, e52067, eLife
Sciences Publications, 2020, doi:10.7554/eLife.52067.
short: M. Narasimhan, A.J. Johnson, R. Prizak, W. Kaufmann, S. Tan, B.E. Casillas
Perez, J. Friml, ELife 9 (2020).
date_created: 2020-02-16T23:00:50Z
date_published: 2020-01-23T00:00:00Z
date_updated: 2025-04-14T07:45:03Z
day: '23'
ddc:
- '570'
- '580'
department:
- _id: JiFr
- _id: GaTk
- _id: EM-Fac
- _id: SyCr
doi: 10.7554/eLife.52067
ec_funded: 1
external_id:
isi:
- '000514104100001'
pmid:
- '31971511'
file:
- access_level: open_access
checksum: 2052daa4be5019534f3a42f200a09f32
content_type: application/pdf
creator: dernst
date_created: 2020-02-18T07:21:16Z
date_updated: 2020-07-14T12:47:59Z
file_id: '7494'
file_name: 2020_eLife_Narasimhan.pdf
file_size: 7247468
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis
in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7497'
abstract:
- lang: eng
text: Endophytic fungi can be beneficial to plant growth. However, the molecular
mechanisms underlying colonization of Acremonium spp. remain unclear. In this
study, a novel endophytic Acremonium strain was isolated from the buds of Panax
notoginseng and named Acremonium sp. D212. The Acremonium sp. D212 could colonize
the roots of P. notoginseng, enhance the resistance of P. notoginseng to root
rot disease, and promote root growth and saponin biosynthesis in P. notoginseng.
Acremonium sp. D212 could secrete indole‐3‐acetic acid (IAA) and jasmonic acid
(JA), and inoculation with the fungus increased the endogenous levels of IAA and
JA in P. notoginseng. Colonization of the Acremonium sp. D212 in the roots of
the rice line Nipponbare was dependent on the concentration of methyl jasmonate
(MeJA) (2 to 15 μM) and 1‐naphthalenacetic acid (NAA) (10 to 20 μM). Moreover,
the roots of the JA signalling‐defective coi1‐18 mutant were colonized by Acremonium
sp. D212 to a lesser degree than those of the wild‐type Nipponbare and miR393b‐overexpressing
lines, and the colonization was rescued by MeJA but not by NAA. It suggests that
the cross‐talk between JA signalling and the auxin biosynthetic pathway plays
a crucial role in the colonization of Acremonium sp. D212 in host plants.
acknowledgement: We thank Professor Jianqiang Wu (Kunming Institute of Botany, Chinese
Academy of Sciences) for providing generous support with the IAA and JA measurements.
We thank Professor Guohua Xu (Nanjing Agricultural University) for generously providing
the Nipponbare rice expressing DR5::GUS. We thank Professor Muyuan Zhu (Zhejiang
University) for generously providing a rice line expressing 35S::miR393b. We thank
Professor Yinong Yang (Pennsylvania State University) for generously providing the
rice line coi1-18. This work was supported by grants from the National Natural Science
Foundation of China (31660501, 31460453, 31860064 and 31470382), the Major Special
Program for Scientific Research, Education Department of Yunnan Province (ZD2015005),
the Project sponsored by SRF for ROCS, SEM ([2013] 1792), the Major Science and
Technique Programs in Yunnan Province (2016ZF001), the Key Projects of the Applied
Basic Research Plan of Yunnan Province (2017FA018), the National Key R&D Program
of China (2018YFD0201100) and the China Agriculture Research System (CARS-21).
article_processing_charge: No
article_type: original
author:
- first_name: L
full_name: Han, L
last_name: Han
- first_name: X
full_name: Zhou, X
last_name: Zhou
- first_name: Y
full_name: Zhao, Y
last_name: Zhao
- first_name: S
full_name: Zhu, S
last_name: Zhu
- first_name: L
full_name: Wu, L
last_name: Wu
- first_name: Y
full_name: He, Y
last_name: He
- first_name: X
full_name: Ping, X
last_name: Ping
- first_name: X
full_name: Lu, X
last_name: Lu
- first_name: W
full_name: Huang, W
last_name: Huang
- first_name: J
full_name: Qian, J
last_name: Qian
- first_name: L
full_name: Zhang, L
last_name: Zhang
- first_name: X
full_name: Jiang, X
last_name: Jiang
- first_name: D
full_name: Zhu, D
last_name: Zhu
- first_name: C
full_name: Luo, C
last_name: Luo
- first_name: S
full_name: Li, S
last_name: Li
- first_name: Q
full_name: Dong, Q
last_name: Dong
- first_name: Q
full_name: Fu, Q
last_name: Fu
- first_name: K
full_name: Deng, K
last_name: Deng
- first_name: X
full_name: Wang, X
last_name: Wang
- first_name: L
full_name: Wang, L
last_name: Wang
- first_name: S
full_name: Peng, S
last_name: Peng
- first_name: J
full_name: Wu, J
last_name: Wu
- first_name: W
full_name: Li, W
last_name: Li
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Y
full_name: Zhu, Y
last_name: Zhu
- first_name: X
full_name: He, X
last_name: He
- first_name: Y
full_name: Du, Y
last_name: Du
citation:
ama: Han L, Zhou X, Zhao Y, et al. Colonization of endophyte Acremonium sp. D212
in Panax notoginseng and rice mediated by auxin and jasmonic acid. Journal
of Integrative Plant Biology. 2020;62(9):1433-1451. doi:10.1111/jipb.12905
apa: Han, L., Zhou, X., Zhao, Y., Zhu, S., Wu, L., He, Y., … Du, Y. (2020). Colonization
of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin
and jasmonic acid. Journal of Integrative Plant Biology. Wiley. https://doi.org/10.1111/jipb.12905
chicago: Han, L, X Zhou, Y Zhao, S Zhu, L Wu, Y He, X Ping, et al. “Colonization
of Endophyte Acremonium Sp. D212 in Panax Notoginseng and Rice Mediated by Auxin
and Jasmonic Acid.” Journal of Integrative Plant Biology. Wiley, 2020.
https://doi.org/10.1111/jipb.12905.
ieee: L. Han et al., “Colonization of endophyte Acremonium sp. D212 in Panax
notoginseng and rice mediated by auxin and jasmonic acid,” Journal of Integrative
Plant Biology, vol. 62, no. 9. Wiley, pp. 1433–1451, 2020.
ista: Han L, Zhou X, Zhao Y, Zhu S, Wu L, He Y, Ping X, Lu X, Huang W, Qian J, Zhang
L, Jiang X, Zhu D, Luo C, Li S, Dong Q, Fu Q, Deng K, Wang X, Wang L, Peng S,
Wu J, Li W, Friml J, Zhu Y, He X, Du Y. 2020. Colonization of endophyte Acremonium
sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid. Journal
of Integrative Plant Biology. 62(9), 1433–1451.
mla: Han, L., et al. “Colonization of Endophyte Acremonium Sp. D212 in Panax Notoginseng
and Rice Mediated by Auxin and Jasmonic Acid.” Journal of Integrative Plant
Biology, vol. 62, no. 9, Wiley, 2020, pp. 1433–51, doi:10.1111/jipb.12905.
short: L. Han, X. Zhou, Y. Zhao, S. Zhu, L. Wu, Y. He, X. Ping, X. Lu, W. Huang,
J. Qian, L. Zhang, X. Jiang, D. Zhu, C. Luo, S. Li, Q. Dong, Q. Fu, K. Deng, X.
Wang, L. Wang, S. Peng, J. Wu, W. Li, J. Friml, Y. Zhu, X. He, Y. Du, Journal
of Integrative Plant Biology 62 (2020) 1433–1451.
date_created: 2020-02-18T10:02:25Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2026-06-18T19:22:29Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/jipb.12905
external_id:
isi:
- '000515803000001'
pmid:
- '31912615'
intvolume: ' 62'
isi: 1
issue: '9'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1111/jipb.12905
month: '09'
oa: 1
oa_version: Published Version
page: 1433-1451
pmid: 1
publication: Journal of Integrative Plant Biology
publication_identifier:
eissn:
- 1744-7909
issn:
- 1672-9072
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice
mediated by auxin and jasmonic acid
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 62
year: '2020'
...
---
_id: '7540'
abstract:
- lang: eng
text: ' In vitro propagation of the ornamentally interesting species Wikstroemia
gemmata is limited by the recalcitrance to form adventitious roots. In this article,
two strategies to improve the rooting capacity of in vitro microcuttings are presented.
Firstly, the effect of exogenous auxin was evaluated in both light and dark cultivated
stem segments and also the sucrose-content of the medium was varied in order to
determine better rooting conditions. Secondly, different spectral lights were
evaluated and the effect on shoot growth and root induction demonstrated that
the exact spectral composition of light is important for successful in vitro growth
and development of Wikstroemia gemmata. We show that exogenous auxin cannot compensate
for the poor rooting under unfavorable light conditions. Adapting the culture
conditions is therefore paramount for successful industrial propagation of Wikstroemia
gemmata. '
article_processing_charge: No
article_type: original
author:
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: H.
full_name: Buyle, H.
last_name: Buyle
- first_name: S.
full_name: Werbrouck, S.
last_name: Werbrouck
- first_name: M.C.
full_name: Van Labeke, M.C.
last_name: Van Labeke
- first_name: D.
full_name: Geelen, D.
last_name: Geelen
citation:
ama: Verstraeten I, Buyle H, Werbrouck S, Van Labeke MC, Geelen D. In vitro shoot
growth and adventitious rooting of Wikstroemia gemmata depends on light quality.
Israel Journal of Plant Sciences. 2020;67(1-2):16-26. doi:10.1163/22238980-20191110
apa: Verstraeten, I., Buyle, H., Werbrouck, S., Van Labeke, M. C., & Geelen,
D. (2020). In vitro shoot growth and adventitious rooting of Wikstroemia gemmata
depends on light quality. Israel Journal of Plant Sciences. Brill. https://doi.org/10.1163/22238980-20191110
chicago: Verstraeten, Inge, H. Buyle, S. Werbrouck, M.C. Van Labeke, and D. Geelen.
“In Vitro Shoot Growth and Adventitious Rooting of Wikstroemia Gemmata Depends
on Light Quality.” Israel Journal of Plant Sciences. Brill, 2020. https://doi.org/10.1163/22238980-20191110.
ieee: I. Verstraeten, H. Buyle, S. Werbrouck, M. C. Van Labeke, and D. Geelen, “In
vitro shoot growth and adventitious rooting of Wikstroemia gemmata depends on
light quality,” Israel Journal of Plant Sciences, vol. 67, no. 1–2. Brill,
pp. 16–26, 2020.
ista: Verstraeten I, Buyle H, Werbrouck S, Van Labeke MC, Geelen D. 2020. In vitro
shoot growth and adventitious rooting of Wikstroemia gemmata depends on light
quality. Israel Journal of Plant Sciences. 67(1–2), 16–26.
mla: Verstraeten, Inge, et al. “In Vitro Shoot Growth and Adventitious Rooting of
Wikstroemia Gemmata Depends on Light Quality.” Israel Journal of Plant Sciences,
vol. 67, no. 1–2, Brill, 2020, pp. 16–26, doi:10.1163/22238980-20191110.
short: I. Verstraeten, H. Buyle, S. Werbrouck, M.C. Van Labeke, D. Geelen, Israel
Journal of Plant Sciences 67 (2020) 16–26.
date_created: 2020-02-28T09:18:01Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-18T06:45:15Z
day: '01'
department:
- _id: JiFr
doi: 10.1163/22238980-20191110
external_id:
isi:
- '000525343300004'
intvolume: ' 67'
isi: 1
issue: 1-2
language:
- iso: eng
month: '02'
oa_version: None
page: 16-26
publication: Israel Journal of Plant Sciences
publication_identifier:
eissn:
- 2223-8980
issn:
- 0792-9978
publication_status: published
publisher: Brill
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vitro shoot growth and adventitious rooting of Wikstroemia gemmata depends
on light quality
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 67
year: '2020'
...
---
_id: '7601'
abstract:
- lang: eng
text: Plasmodesmata (PD) are crucial structures for intercellular communication
in multicellular plants with remorins being their crucial plant-specific structural
and functional constituents. The PD biogenesis is an intriguing but poorly understood
process. By expressing an Arabidopsis remorin protein in mammalian cells, we have
reconstituted a PD-like filamentous structure, termed remorin filament (RF), connecting
neighboring cells physically and physiologically. Notably, RFs are capable of
transporting macromolecules intercellularly, in a way similar to plant PD. With
further super-resolution microscopic analysis and biochemical characterization,
we found that RFs are also composed of actin filaments, forming the core skeleton
structure, aligned with the remorin protein. This unique heterologous filamentous
structure might explain the molecular mechanism for remorin function as well as
PD construction. Furthermore, remorin protein exhibits a specific distribution
manner in the plasma membrane in mammalian cells, representing a lipid nanodomain,
depending on its lipid modification status. Our studies not only provide crucial
insights into the mechanism of PD biogenesis, but also uncovers unsuspected fundamental
mechanistic and evolutionary links between intercellular communication systems
of plants and animals.
article_processing_charge: No
author:
- first_name: Zhuang
full_name: Wei, Zhuang
last_name: Wei
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Tao
full_name: Liu, Tao
last_name: Liu
- first_name: Yuan
full_name: Wu, Yuan
last_name: Wu
- first_name: Ji-Gang
full_name: Lei, Ji-Gang
last_name: Lei
- first_name: ZhengJun
full_name: Chen, ZhengJun
last_name: Chen
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Hong-Wei
full_name: Xue, Hong-Wei
last_name: Xue
- first_name: Kan
full_name: Liao, Kan
last_name: Liao
citation:
ama: Wei Z, Tan S, Liu T, et al. Plasmodesmata-like intercellular connections by
plant remorin in animal cells. bioRxiv. 2020. doi:10.1101/791137
apa: Wei, Z., Tan, S., Liu, T., Wu, Y., Lei, J.-G., Chen, Z., … Liao, K. (2020).
Plasmodesmata-like intercellular connections by plant remorin in animal cells.
bioRxiv. https://doi.org/10.1101/791137
chicago: Wei, Zhuang, Shutang Tan, Tao Liu, Yuan Wu, Ji-Gang Lei, ZhengJun Chen,
Jiří Friml, Hong-Wei Xue, and Kan Liao. “Plasmodesmata-like Intercellular Connections
by Plant Remorin in Animal Cells.” BioRxiv, 2020. https://doi.org/10.1101/791137.
ieee: Z. Wei et al., “Plasmodesmata-like intercellular connections by plant
remorin in animal cells,” bioRxiv. 2020.
ista: Wei Z, Tan S, Liu T, Wu Y, Lei J-G, Chen Z, Friml J, Xue H-W, Liao K. 2020.
Plasmodesmata-like intercellular connections by plant remorin in animal cells.
bioRxiv, 10.1101/791137.
mla: Wei, Zhuang, et al. “Plasmodesmata-like Intercellular Connections by Plant
Remorin in Animal Cells.” BioRxiv, 2020, doi:10.1101/791137.
short: Z. Wei, S. Tan, T. Liu, Y. Wu, J.-G. Lei, Z. Chen, J. Friml, H.-W. Xue, K.
Liao, BioRxiv (2020).
das_tickbox: '1'
date_created: 2020-03-21T16:34:42Z
date_published: 2020-02-19T00:00:00Z
date_updated: 2026-07-06T12:53:37Z
day: '19'
department:
- _id: JiFr
doi: 10.1101/791137
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/791137
month: '02'
oa: 1
oa_version: Preprint
page: '22'
publication: bioRxiv
publication_status: published
status: public
title: Plasmodesmata-like intercellular connections by plant remorin in animal cells
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
OA_place: publisher
_id: '8589'
abstract:
- lang: eng
text: The plant hormone auxin plays indispensable roles in plant growth and development.
An essential level of regulation in auxin action is the directional auxin transport
within cells. The establishment of auxin gradient in plant tissue has been attributed
to local auxin biosynthesis and directional intercellular auxin transport, which
both are controlled by various environmental and developmental signals. It is
well established that asymmetric auxin distribution in cells is achieved by polarly
localized PIN-FORMED (PIN) auxin efflux transporters. Despite the initial insights
into cellular mechanisms of PIN polarization obtained from the last decades, the
molecular mechanism and specific regulators mediating PIN polarization remains
elusive. In this thesis, we aim to find novel players in PIN subcellular polarity
regulation during Arabidopsis development. We first characterize the physiological
effect of piperonylic acid (PA) on Arabidopsis hypocotyl gravitropic bending and
PIN polarization. Secondly, we reveal the importance of SCFTIR1/AFB auxin signaling
pathway in shoot gravitropism bending termination. In addition, we also explore
the role of myosin XI complex, and actin cytoskeleton in auxin feedback regulation
on PIN polarity. In Chapter 1, we give an overview of the current knowledge about
PIN-mediated auxin fluxes in various plant tropic responses. In Chapter 2, we
study the physiological effect of PA on shoot gravitropic bending. Our results
show that PA treatment inhibits auxin-mediated PIN3 repolarization by interfering
with PINOID and PIN3 phosphorylation status, ultimately leading to hyperbending
hypocotyls. In Chapter 3, we provide evidence to show that the SCFTIR1/AFB nuclear
auxin signaling pathway is crucial and required for auxin-mediated PIN3 repolarization
and shoot gravitropic bending termination. In Chapter 4, we perform a phosphoproteomics
approach and identify the motor protein Myosin XI and its binding protein, the
MadB2 family, as an essential regulator of PIN polarity for auxin-canalization
related developmental processes. In Chapter 5, we demonstrate the vital role of
actin cytoskeleton in auxin feedback on PIN polarity by regulating PIN subcellular
trafficking. Overall, the data presented in this PhD thesis brings novel insights
into the PIN polar localization regulation that resulted in the (re)establishment
of the polar auxin flow and gradient in response to environmental stimuli during
plant development.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: I also want to thank the China Scholarship Council for supporting
my study during the year from 2015 to 2019. I also want to thank IST facilities
– the Bioimaging facility, the media kitchen, the plant facility and all of the
campus services, for their support.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
citation:
ama: Han H. Novel insights into PIN polarity regulation during Arabidopsis development.
2020. doi:10.15479/AT:ISTA:8589
apa: Han, H. (2020). Novel insights into PIN polarity regulation during Arabidopsis
development. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:8589
chicago: Han, Huibin. “Novel Insights into PIN Polarity Regulation during Arabidopsis
Development.” Institute of Science and Technology Austria, 2020. https://doi.org/10.15479/AT:ISTA:8589.
ieee: H. Han, “Novel insights into PIN polarity regulation during Arabidopsis development,”
Institute of Science and Technology Austria, 2020.
ista: Han H. 2020. Novel insights into PIN polarity regulation during Arabidopsis
development. Institute of Science and Technology Austria.
mla: Han, Huibin. Novel Insights into PIN Polarity Regulation during Arabidopsis
Development. Institute of Science and Technology Austria, 2020, doi:10.15479/AT:ISTA:8589.
short: H. Han, Novel Insights into PIN Polarity Regulation during Arabidopsis Development,
Institute of Science and Technology Austria, 2020.
corr_author: '1'
date_created: 2020-09-30T14:50:51Z
date_published: 2020-09-30T00:00:00Z
date_updated: 2026-07-28T12:51:58Z
day: '30'
ddc:
- '580'
degree_awarded: PhD
department:
- _id: JiFr
doi: 10.15479/AT:ISTA:8589
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checksum: c4bda1947d4c09c428ac9ce667b02327
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creator: dernst
date_created: 2020-09-30T14:50:20Z
date_updated: 2020-09-30T14:50:20Z
file_id: '8590'
file_name: 2020_Han_Thesis.docx
file_size: 49198118
relation: source_file
- access_level: open_access
checksum: 3f4f5d1718c2230adf30639ecaf8a00b
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creator: dernst
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language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: '164'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
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status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Novel insights into PIN polarity regulation during Arabidopsis development
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2020'
...
---
OA_place: publisher
OA_type: free access
_id: '7643'
acknowledgement: This work was supported by the European Research Council under the
European Union’s Horizon 2020 research and innovation Programme (ERC grant agreement
number 742985), and the Austrian Science Fund (FWF, grant number I 3630-B25) to
JF. HH is supported by the China Scholarship Council (CSC scholarship). We thank
Keiko U. Torii (University of Washington/Nagoya University), Mark Estelle (University
of California San Diego), Ottoline Leyser (Sainsbury Laboratory, University of Cambridge),
and Yunde Zhao (University of California San Diego) for sharing published genetic
lines. We also thank Dr. Maciek Adamowski (Institute of Science and Technology Austria)
for critical reading of the manuscript.
article_processing_charge: No
article_type: letter_editor
author:
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Hana
full_name: Rakusova, Hana
id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA
last_name: Rakusova
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Han H, Rakusova H, Verstraeten I, Zhang Y, Friml J. SCF TIR1/AFB auxin signaling
for bending termination during shoot gravitropism. Plant Physiology. 2020;183(5):37-40.
doi:10.1104/pp.20.00212
apa: Han, H., Rakusova, H., Verstraeten, I., Zhang, Y., & Friml, J. (2020).
SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism.
Plant Physiology. American Society of Plant Biologists. https://doi.org/10.1104/pp.20.00212
chicago: Han, Huibin, Hana Rakusova, Inge Verstraeten, Yuzhou Zhang, and Jiří Friml.
“SCF TIR1/AFB Auxin Signaling for Bending Termination during Shoot Gravitropism.”
Plant Physiology. American Society of Plant Biologists, 2020. https://doi.org/10.1104/pp.20.00212.
ieee: H. Han, H. Rakusova, I. Verstraeten, Y. Zhang, and J. Friml, “SCF TIR1/AFB
auxin signaling for bending termination during shoot gravitropism,” Plant Physiology,
vol. 183, no. 5. American Society of Plant Biologists, pp. 37–40, 2020.
ista: Han H, Rakusova H, Verstraeten I, Zhang Y, Friml J. 2020. SCF TIR1/AFB auxin
signaling for bending termination during shoot gravitropism. Plant Physiology.
183(5), 37–40.
mla: Han, Huibin, et al. “SCF TIR1/AFB Auxin Signaling for Bending Termination during
Shoot Gravitropism.” Plant Physiology, vol. 183, no. 5, American Society
of Plant Biologists, 2020, pp. 37–40, doi:10.1104/pp.20.00212.
short: H. Han, H. Rakusova, I. Verstraeten, Y. Zhang, J. Friml, Plant Physiology
183 (2020) 37–40.
corr_author: '1'
date_created: 2020-04-06T10:06:40Z
date_published: 2020-05-08T00:00:00Z
date_updated: 2026-07-28T12:51:59Z
day: '08'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1104/pp.20.00212
ec_funded: 1
external_id:
isi:
- '000536641800018'
pmid:
- '32107280'
intvolume: ' 183'
isi: 1
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1104/pp.20.00212
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oa: 1
oa_version: Published Version
page: 37-40
pmid: 1
project:
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call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
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grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Physiology
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eissn:
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publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
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status: public
scopus_import: '1'
status: public
title: SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 183
year: '2020'
...
---
OA_place: publisher
OA_type: hybrid
_id: '8002'
abstract:
- lang: eng
text: Wound healing in plant tissues, consisting of rigid cell wall-encapsulated
cells, represents a considerable challenge and occurs through largely unknown
mechanisms distinct from those in animals. Owing to their inability to migrate,
plant cells rely on targeted cell division and expansion to regenerate wounds.
Strict coordination of these wound-induced responses is essential to ensure efficient,
spatially restricted wound healing. Single-cell tracking by live imaging allowed
us to gain mechanistic insight into the wound perception and coordination of wound
responses after laser-based wounding in Arabidopsis root. We revealed a crucial
contribution of the collapse of damaged cells in wound perception and detected
an auxin increase specific to cells immediately adjacent to the wound. This localized
auxin increase balances wound-induced cell expansion and restorative division
rates in a dose-dependent manner, leading to tumorous overproliferation when the
canonical TIR1 auxin signaling is disrupted. Auxin and wound-induced turgor pressure
changes together also spatially define the activation of key components of regeneration,
such as the transcription regulator ERF115. Our observations suggest that the
wound signaling involves the sensing of collapse of damaged cells and a local
auxin signaling activation to coordinate the downstream transcriptional responses
in the immediate wound vicinity.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
article_number: '202003346'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
orcid: 0000-0001-6111-9353
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J.
Wounding-induced changes in cellular pressure and localized auxin signalling spatially
coordinate restorative divisions in roots. Proceedings of the National Academy
of Sciences of the United States of America. 2020;117(26). doi:10.1073/pnas.2003346117
apa: Hörmayer, L., Montesinos López, J. C., Marhavá, P., Benková, E., Yoshida, S.,
& Friml, J. (2020). Wounding-induced changes in cellular pressure and localized
auxin signalling spatially coordinate restorative divisions in roots. Proceedings
of the National Academy of Sciences of the United States of America. National
Academy of Sciences. https://doi.org/10.1073/pnas.2003346117
chicago: Hörmayer, Lukas, Juan C Montesinos López, Petra Marhavá, Eva Benková, Saiko
Yoshida, and Jiří Friml. “Wounding-Induced Changes in Cellular Pressure and Localized
Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.” Proceedings
of the National Academy of Sciences of the United States of America. National
Academy of Sciences, 2020. https://doi.org/10.1073/pnas.2003346117.
ieee: L. Hörmayer, J. C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, and
J. Friml, “Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots,” Proceedings of the National
Academy of Sciences of the United States of America, vol. 117, no. 26. National
Academy of Sciences, 2020.
ista: Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J.
2020. Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots. Proceedings of the National
Academy of Sciences of the United States of America. 117(26), 202003346.
mla: Hörmayer, Lukas, et al. “Wounding-Induced Changes in Cellular Pressure and
Localized Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.”
Proceedings of the National Academy of Sciences of the United States of America,
vol. 117, no. 26, 202003346, National Academy of Sciences, 2020, doi:10.1073/pnas.2003346117.
short: L. Hörmayer, J.C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, J.
Friml, Proceedings of the National Academy of Sciences of the United States of
America 117 (2020).
corr_author: '1'
date_created: 2020-06-22T13:33:52Z
date_published: 2020-06-30T00:00:00Z
date_updated: 2026-08-14T22:30:25Z
day: '30'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1073/pnas.2003346117
ec_funded: 1
external_id:
isi:
- '000565729700033'
pmid:
- '32541049'
file:
- access_level: open_access
checksum: 908b09437680181de9990915f2113aca
content_type: application/pdf
creator: dernst
date_created: 2020-06-23T11:30:53Z
date_updated: 2020-07-14T12:48:07Z
file_id: '8009'
file_name: 2020_PNAS_Hoermayer.pdf
file_size: 2407102
relation: main_file
file_date_updated: 2020-07-14T12:48:07Z
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intvolume: ' 117'
isi: 1
issue: '26'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/how-wounded-plants-coordinate-their-healing/
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 117
year: '2020'
...
---
_id: '8986'
abstract:
- lang: eng
text: 'Flowering plants display the highest diversity among plant species and have
notably shaped terrestrial landscapes. Nonetheless, the evolutionary origin of
their unprecedented morphological complexity remains largely an enigma. Here,
we show that the coevolution of cis-regulatory and coding regions of PIN-FORMED
(PIN) auxin transporters confined their expression to certain cell types and directed
their subcellular localization to particular cell sides, which together enabled
dynamic auxin gradients across tissues critical to the complex architecture of
flowering plants. Extensive intraspecies and interspecies genetic complementation
experiments with PINs from green alga up to flowering plant lineages showed that
PIN genes underwent three subsequent, critical evolutionary innovations and thus
acquired a triple function to regulate the development of three essential components
of the flowering plant Arabidopsis: shoot/root, inflorescence, and floral organ.
Our work highlights the critical role of functional innovations within the PIN
gene family as essential prerequisites for the origin of flowering plants.'
acknowledgement: 'We thank C.Löhne (Botanic Gardens, University of Bonn) for providing
us with A. trichopoda. We would like to thank T.Han, A.Mally (IST, Austria), and
C.Hartinger (University of Oxford) for constructive comment and careful reading.
Funding: The research leading to these results has received funding from the European
Union’s Horizon 2020 Research and Innovation Programme (ERC grant agreement number
742985), Austrian Science Fund (FWF, grant number I 3630-B25), DOC Fellowship of
the Austrian Academy of Sciences, and IST Fellow program. '
article_number: eabc8895
article_processing_charge: No
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Rodriguez Solovey L, Li L, Zhang X, Friml J. Functional innovations
of PIN auxin transporters mark crucial evolutionary transitions during rise of
flowering plants. Science Advances. 2020;6(50). doi:10.1126/sciadv.abc8895
apa: Zhang, Y., Rodriguez Solovey, L., Li, L., Zhang, X., & Friml, J. (2020).
Functional innovations of PIN auxin transporters mark crucial evolutionary transitions
during rise of flowering plants. Science Advances. AAAS. https://doi.org/10.1126/sciadv.abc8895
chicago: Zhang, Yuzhou, Lesia Rodriguez Solovey, Lanxin Li, Xixi Zhang, and Jiří
Friml. “Functional Innovations of PIN Auxin Transporters Mark Crucial Evolutionary
Transitions during Rise of Flowering Plants.” Science Advances. AAAS, 2020.
https://doi.org/10.1126/sciadv.abc8895.
ieee: Y. Zhang, L. Rodriguez Solovey, L. Li, X. Zhang, and J. Friml, “Functional
innovations of PIN auxin transporters mark crucial evolutionary transitions during
rise of flowering plants,” Science Advances, vol. 6, no. 50. AAAS, 2020.
ista: Zhang Y, Rodriguez Solovey L, Li L, Zhang X, Friml J. 2020. Functional innovations
of PIN auxin transporters mark crucial evolutionary transitions during rise of
flowering plants. Science Advances. 6(50), eabc8895.
mla: Zhang, Yuzhou, et al. “Functional Innovations of PIN Auxin Transporters Mark
Crucial Evolutionary Transitions during Rise of Flowering Plants.” Science
Advances, vol. 6, no. 50, eabc8895, AAAS, 2020, doi:10.1126/sciadv.abc8895.
short: Y. Zhang, L. Rodriguez Solovey, L. Li, X. Zhang, J. Friml, Science Advances
6 (2020).
corr_author: '1'
date_created: 2021-01-03T23:01:23Z
date_published: 2020-12-11T00:00:00Z
date_updated: 2026-08-14T22:30:48Z
day: '11'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1126/sciadv.abc8895
ec_funded: 1
external_id:
isi:
- '000599903600014'
pmid:
- '33310852'
file:
- access_level: open_access
checksum: 5ac2500b191c08ef6dab5327f40ff663
content_type: application/pdf
creator: dernst
date_created: 2021-01-07T12:44:33Z
date_updated: 2021-01-07T12:44:33Z
file_id: '8994'
file_name: 2020_ScienceAdvances_Zhang.pdf
file_size: 10578145
relation: main_file
success: 1
file_date_updated: 2021-01-07T12:44:33Z
has_accepted_license: '1'
intvolume: ' 6'
isi: 1
issue: '50'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: AAAS
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Functional innovations of PIN auxin transporters mark crucial evolutionary
transitions during rise of flowering plants
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 6
year: '2020'
...
---
_id: '8283'
abstract:
- lang: eng
text: 'Drought and salt stress are the main environmental cues affecting the survival,
development, distribution, and yield of crops worldwide. MYB transcription factors
play a crucial role in plants’ biological processes, but the function of pineapple
MYB genes is still obscure. In this study, one of the pineapple MYB transcription
factors, AcoMYB4, was isolated and characterized. The results showed that AcoMYB4
is localized in the cell nucleus, and its expression is induced by low temperature,
drought, salt stress, and hormonal stimulation, especially by abscisic acid (ABA).
Overexpression of AcoMYB4 in rice and Arabidopsis enhanced plant sensitivity to
osmotic stress; it led to an increase in the number stomata on leaf surfaces and
lower germination rate under salt and drought stress. Furthermore, in AcoMYB4
OE lines, the membrane oxidation index, free proline, and soluble sugar contents
were decreased. In contrast, electrolyte leakage and malondialdehyde (MDA) content
increased significantly due to membrane injury, indicating higher sensitivity
to drought and salinity stresses. Besides the above, both the expression level
and activities of several antioxidant enzymes were decreased, indicating lower
antioxidant activity in AcoMYB4 transgenic plants. Moreover, under osmotic stress,
overexpression of AcoMYB4 inhibited ABA biosynthesis through a decrease in the
transcription of genes responsible for ABA synthesis (ABA1 and ABA2) and ABA signal
transduction factor ABI5. These results suggest that AcoMYB4 negatively regulates
osmotic stress by attenuating cellular ABA biosynthesis and signal transduction
pathways. '
acknowledgement: 'We would like to thank the reviewers for their helpful comments
on the original manuscript. '
article_number: '5272'
article_processing_charge: No
article_type: original
author:
- first_name: Huihuang
full_name: Chen, Huihuang
last_name: Chen
- first_name: Linyi
full_name: Lai, Linyi
last_name: Lai
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Liping
full_name: Liu, Liping
last_name: Liu
- first_name: Bello Hassan
full_name: Jakada, Bello Hassan
last_name: Jakada
- first_name: Youmei
full_name: Huang, Youmei
last_name: Huang
- first_name: Qing
full_name: He, Qing
last_name: He
- first_name: Mengnan
full_name: Chai, Mengnan
last_name: Chai
- first_name: Xiaoping
full_name: Niu, Xiaoping
last_name: Niu
- first_name: Yuan
full_name: Qin, Yuan
last_name: Qin
citation:
ama: Chen H, Lai L, Li L, et al. AcoMYB4, an Ananas comosus L. MYB transcription
factor, functions in osmotic stress through negative regulation of ABA signaling.
International Journal of Molecular Sciences. 2020;21(16). doi:10.3390/ijms21165727
apa: Chen, H., Lai, L., Li, L., Liu, L., Jakada, B. H., Huang, Y., … Qin, Y. (2020).
AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress
through negative regulation of ABA signaling. International Journal of Molecular
Sciences. MDPI. https://doi.org/10.3390/ijms21165727
chicago: Chen, Huihuang, Linyi Lai, Lanxin Li, Liping Liu, Bello Hassan Jakada,
Youmei Huang, Qing He, Mengnan Chai, Xiaoping Niu, and Yuan Qin. “AcoMYB4, an
Ananas Comosus L. MYB Transcription Factor, Functions in Osmotic Stress through
Negative Regulation of ABA Signaling.” International Journal of Molecular Sciences.
MDPI, 2020. https://doi.org/10.3390/ijms21165727.
ieee: H. Chen et al., “AcoMYB4, an Ananas comosus L. MYB transcription factor,
functions in osmotic stress through negative regulation of ABA signaling,” International
Journal of Molecular Sciences, vol. 21, no. 16. MDPI, 2020.
ista: Chen H, Lai L, Li L, Liu L, Jakada BH, Huang Y, He Q, Chai M, Niu X, Qin Y.
2020. AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic
stress through negative regulation of ABA signaling. International Journal of
Molecular Sciences. 21(16), 5272.
mla: Chen, Huihuang, et al. “AcoMYB4, an Ananas Comosus L. MYB Transcription Factor,
Functions in Osmotic Stress through Negative Regulation of ABA Signaling.” International
Journal of Molecular Sciences, vol. 21, no. 16, 5272, MDPI, 2020, doi:10.3390/ijms21165727.
short: H. Chen, L. Lai, L. Li, L. Liu, B.H. Jakada, Y. Huang, Q. He, M. Chai, X.
Niu, Y. Qin, International Journal of Molecular Sciences 21 (2020).
date_created: 2020-08-24T06:24:03Z
date_published: 2020-08-10T00:00:00Z
date_updated: 2026-08-14T22:30:48Z
day: '10'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.3390/ijms21165727
external_id:
isi:
- '000565090300001'
pmid:
- '32785037'
file:
- access_level: open_access
checksum: 03b039244e6ae80580385fd9f577e2b2
content_type: application/pdf
creator: cziletti
date_created: 2020-08-25T09:53:50Z
date_updated: 2020-08-25T09:53:50Z
file_id: '8292'
file_name: 2020_IntMolecSciences_Chen.pdf
file_size: 5718755
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file_date_updated: 2020-08-25T09:53:50Z
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intvolume: ' 21'
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issue: '16'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
issn:
- 1661-6596
publication_status: published
publisher: MDPI
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic
stress through negative regulation of ABA signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 21
year: '2020'
...
---
_id: '8139'
abstract:
- lang: eng
text: 'Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated
in many aspects of plant growth, development, intra- and inter-cellular signaling,
nutrient uptake and pathogen defense. Despite these significant roles, little
is known about the precise molecular details of how it functions in planta. In
order to facilitate the direct quantitative study of plant CME, here we review
current routinely used methods and present refined, standardized quantitative
imaging protocols which allow the detailed characterization of CME at multiple
scales in plant tissues. These include: (i) an efficient electron microscopy protocol
for the imaging of Arabidopsis CME vesicles in situ, thus providing a method for
the detailed characterization of the ultra-structure of clathrin-coated vesicles;
(ii) a detailed protocol and analysis for quantitative live-cell fluorescence
microscopy to precisely examine the temporal interplay of endocytosis components
during single CME events; (iii) a semi-automated analysis to allow the quantitative
characterization of global internalization of cargos in whole plant tissues; and
(iv) an overview and validation of useful genetic and pharmacological tools to
interrogate the molecular mechanisms and function of CME in intact plant samples.'
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
acknowledgement: "This paper is dedicated to the memory of Christien Merrifield. He
pioneered quantitative\r\nimaging approaches in mammalian CME and his mentorship
inspired the development of all\r\nthe analysis methods presented here. His joy
in research, pure scientific curiosity and\r\nmicroscopy excellence remain a constant
inspiration. We thank Daniel Van Damme for gifting\r\nus the CLC2-GFP x TPLATE-TagRFP
plants used in this manuscript. We further thank the\r\nScientific Service Units
at IST Austria; specifically, the Electron Microscopy Facility for\r\ntechnical
assistance (in particular Vanessa Zheden) and the BioImaging Facility BioImaging\r\nFacility
for access to equipment. "
article_number: jcs248062
article_processing_charge: No
article_type: original
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: G
full_name: Vert, G
last_name: Vert
- first_name: SY
full_name: Bednarek, SY
last_name: Bednarek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Johnson AJ, Gnyliukh N, Kaufmann W, et al. Experimental toolbox for quantitative
evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal
of Cell Science. 2020;133(15). doi:10.1242/jcs.248062
apa: Johnson, A. J., Gnyliukh, N., Kaufmann, W., Narasimhan, M., Vert, G., Bednarek,
S., & Friml, J. (2020). Experimental toolbox for quantitative evaluation of
clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell
Science. The Company of Biologists. https://doi.org/10.1242/jcs.248062
chicago: Johnson, Alexander J, Nataliia Gnyliukh, Walter Kaufmann, Madhumitha Narasimhan,
G Vert, SY Bednarek, and Jiří Friml. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science. The Company of Biologists, 2020. https://doi.org/10.1242/jcs.248062.
ieee: A. J. Johnson et al., “Experimental toolbox for quantitative evaluation
of clathrin-mediated endocytosis in the plant model Arabidopsis,” Journal of
Cell Science, vol. 133, no. 15. The Company of Biologists, 2020.
ista: Johnson AJ, Gnyliukh N, Kaufmann W, Narasimhan M, Vert G, Bednarek S, Friml
J. 2020. Experimental toolbox for quantitative evaluation of clathrin-mediated
endocytosis in the plant model Arabidopsis. Journal of Cell Science. 133(15),
jcs248062.
mla: Johnson, Alexander J., et al. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science, vol. 133, no. 15, jcs248062, The Company of Biologists, 2020,
doi:10.1242/jcs.248062.
short: A.J. Johnson, N. Gnyliukh, W. Kaufmann, M. Narasimhan, G. Vert, S. Bednarek,
J. Friml, Journal of Cell Science 133 (2020).
date_created: 2020-07-21T08:58:19Z
date_published: 2020-08-06T00:00:00Z
date_updated: 2026-08-14T22:30:56Z
day: '06'
ddc:
- '575'
department:
- _id: JiFr
- _id: EM-Fac
doi: 10.1242/jcs.248062
ec_funded: 1
external_id:
isi:
- '000561047900021'
pmid:
- '32616560'
file:
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checksum: 2d11f79a0b4e0a380fb002b933da331a
content_type: application/pdf
creator: ajohnson
date_created: 2020-11-26T17:12:51Z
date_updated: 2021-08-08T22:30:03Z
embargo: 2021-08-07
file_id: '8815'
file_name: 2020 - Johnson - JSC - plant CME toolbox.pdf
file_size: 15150403
relation: main_file
file_date_updated: 2021-08-08T22:30:03Z
has_accepted_license: '1'
intvolume: ' 133'
isi: 1
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
record:
- id: '14510'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis
in the plant model Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 133
year: '2020'
...
---
_id: '7427'
abstract:
- lang: eng
text: Plants, like other multicellular organisms, survive through a delicate balance
between growth and defense against pathogens. Salicylic acid (SA) is a major defense
signal in plants, and the perception mechanism as well as downstream signaling
activating the immune response are known. Here, we identify a parallel SA signaling
that mediates growth attenuation. SA directly binds to A subunits of protein phosphatase
2A (PP2A), inhibiting activity of this complex. Among PP2A targets, the PIN2 auxin
transporter is hyperphosphorylated in response to SA, leading to changed activity
of this important growth regulator. Accordingly, auxin transport and auxin-mediated
root development, including growth, gravitropic response, and lateral root organogenesis,
are inhibited. This study reveals how SA, besides activating immunity, concomitantly
attenuates growth through crosstalk with the auxin distribution network. Further
analysis of this dual role of SA and characterization of additional SA-regulated
PP2A targets will provide further insights into mechanisms maintaining a balance
between growth and defense.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Shigeyuki Betsuyaku (University of Tsukuba), Alison Delong
(Brown University), Xinnian Dong (Duke University), Dolf Weijers (Wageningen University),
Yuelin Zhang (UBC), and Martine Pastuglia (Institut Jean-Pierre Bourgin) for sharing
published materials; Jana Riederer for help with cantharidin physiological analysis;
David Domjan for help with cloning pET28a-PIN2HL; Qing Lu for help with DARTS; Hana
Kozubı´kova´ for technical support on SA derivative synthesis; Zuzana Vondra´ kova´
for technical support with tobacco cells; Lucia Strader (Washington University),
Bert De Rybel (Ghent University), Bartel Vanholme (Ghent University), and Lukas
Mach (BOKU) for helpful discussions; and bioimaging and life science facilities
of IST Austria for continuous support. We gratefully acknowledge the Nottingham
Arabidopsis Stock Center (NASC) for providing T-DNA insertional mutants. The DSC
and SPR instruments were provided by the EQ-BOKU VIBT GmbH and the BOKU Core Facility
for Biomolecular and Cellular Analysis, with help of Irene Schaffner. The research
leading to these results has received funding from the European Union’s Horizon
2020 program (ERC grant agreement no. 742985 to J.F.) and the People Programme (Marie
Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013)
under REA grant agreement no. 291734. S.T. was supported by a European Molecular
Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). O.N.
was supported by the Ministry of Education, Youth and Sports of the Czech Republic
(European Regional Development Fund-Project ‘‘Centre for Experimental Plant Biology’’
no. CZ.02.1.01/0.0/0.0/16_019/0000738). J. Pospısil was supported by European Regional
Development Fund Project ‘‘Centre for Experimental Plant Biology’’\r\n(no. CZ.02.1.01/0.0/0.0/16_019/0000738).
J. Petrasek was supported by EU Operational Programme Prague-Competitiveness (no.
CZ.2.16/3.1.00/21519). "
article_processing_charge: No
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Melinda F
full_name: Abas, Melinda F
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Pavel
full_name: Lasák, Pavel
last_name: Lasák
- first_name: Ivan
full_name: Petřík, Ivan
last_name: Petřík
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Pospíšil, Jiří
last_name: Pospíšil
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tan S, Abas MF, Verstraeten I, et al. Salicylic acid targets protein phosphatase
2A to attenuate growth in plants. Current Biology. 2020;30(3):381-395.e8.
doi:10.1016/j.cub.2019.11.058
apa: Tan, S., Abas, M. F., Verstraeten, I., Glanc, M., Molnar, G., Hajny, J., …
Friml, J. (2020). Salicylic acid targets protein phosphatase 2A to attenuate growth
in plants. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2019.11.058
chicago: Tan, Shutang, Melinda F Abas, Inge Verstraeten, Matous Glanc, Gergely Molnar,
Jakub Hajny, Pavel Lasák, et al. “Salicylic Acid Targets Protein Phosphatase 2A
to Attenuate Growth in Plants.” Current Biology. Cell Press, 2020. https://doi.org/10.1016/j.cub.2019.11.058.
ieee: S. Tan et al., “Salicylic acid targets protein phosphatase 2A to attenuate
growth in plants,” Current Biology, vol. 30, no. 3. Cell Press, p. 381–395.e8,
2020.
ista: Tan S, Abas MF, Verstraeten I, Glanc M, Molnar G, Hajny J, Lasák P, Petřík
I, Russinova E, Petrášek J, Novák O, Pospíšil J, Friml J. 2020. Salicylic acid
targets protein phosphatase 2A to attenuate growth in plants. Current Biology.
30(3), 381–395.e8.
mla: Tan, Shutang, et al. “Salicylic Acid Targets Protein Phosphatase 2A to Attenuate
Growth in Plants.” Current Biology, vol. 30, no. 3, Cell Press, 2020, p.
381–395.e8, doi:10.1016/j.cub.2019.11.058.
short: S. Tan, M.F. Abas, I. Verstraeten, M. Glanc, G. Molnar, J. Hajny, P. Lasák,
I. Petřík, E. Russinova, J. Petrášek, O. Novák, J. Pospíšil, J. Friml, Current
Biology 30 (2020) 381–395.e8.
corr_author: '1'
date_created: 2020-02-02T23:01:00Z
date_published: 2020-02-03T00:00:00Z
date_updated: 2026-08-14T22:30:58Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cub.2019.11.058
ec_funded: 1
external_id:
isi:
- '000511287900018'
pmid:
- '31956021'
file:
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checksum: 16f7d51fe28f91c21e4896a2028df40b
content_type: application/pdf
creator: dernst
date_created: 2020-09-22T09:51:28Z
date_updated: 2020-09-22T09:51:28Z
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success: 1
file_date_updated: 2020-09-22T09:51:28Z
has_accepted_license: '1'
intvolume: ' 30'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 381-395.e8
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
grant_number: 723-2015
name: Molecular Mechanism underlying Salicylic Acid Regulation of Endocytic Trafficking
in Arabidopsis
publication: Current Biology
publication_identifier:
issn:
- '09609822'
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Salicylic acid targets protein phosphatase 2A to attenuate growth in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 30
year: '2020'
...