---
_id: '1996'
abstract:
- lang: eng
text: Auxin polar transport, local maxima, and gradients have become an importantmodel
system for studying self-organization. Auxin distribution is regulated by auxin-dependent
positive feedback loops that are not well-understood at the molecular level. Previously,
we showed the involvement of the RHO of Plants (ROP) effector INTERACTOR of CONSTITUTIVELY
active ROP 1 (ICR1) in regulation of auxin transport and that ICR1 levels are
posttranscriptionally repressed at the site of maximum auxin accumulation at the
root tip. Here, we show that bimodal regulation of ICR1 levels by auxin is essential
for regulating formation of auxin local maxima and gradients. ICR1 levels increase
concomitant with increase in auxin response in lateral root primordia, cotyledon
tips, and provascular tissues. However, in the embryo hypophysis and root meristem,
when auxin exceeds critical levels, ICR1 is rapidly destabilized by an SCF(TIR1/AFB)
[SKP, Cullin, F-box (transport inhibitor response 1/auxin signaling F-box protein)]-dependent
auxin signaling mechanism. Furthermore, ectopic expression of ICR1 in the embryo
hypophysis resulted in reduction of auxin accumulation and concomitant root growth
arrest. ICR1 disappeared during root regeneration and lateral root initiation
concomitantly with the formation of a local auxin maximum in response to external
auxin treatments and transiently after gravitropic stimulation. Destabilization
of ICR1 was impaired after inhibition of auxin transport and signaling, proteasome
function, and protein synthesis. A mathematical model based on these findings
shows that an in vivo-like auxin distribution, rootward auxin flux, and shootward
reflux can be simulated without assuming preexisting tissue polarity. Our experimental
results and mathematical modeling indicate that regulation of auxin distribution
is tightly associated with auxin-dependent ICR1 levels.
article_processing_charge: No
author:
- first_name: Ora
full_name: Hazak, Ora
last_name: Hazak
- first_name: Uri
full_name: Obolski, Uri
last_name: Obolski
- first_name: Tomas
full_name: Prat, Tomas
id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
last_name: Prat
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Lilach
full_name: Hadany, Lilach
last_name: Hadany
- first_name: Shaul
full_name: Yalovsky, Shaul
last_name: Yalovsky
citation:
ama: Hazak O, Obolski U, Prat T, Friml J, Hadany L, Yalovsky S. Bimodal regulation
of ICR1 levels generates self-organizing auxin distribution. PNAS. 2014;111(50):E5471-E5479.
doi:10.1073/pnas.1413918111
apa: Hazak, O., Obolski, U., Prat, T., Friml, J., Hadany, L., & Yalovsky, S.
(2014). Bimodal regulation of ICR1 levels generates self-organizing auxin distribution.
PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1413918111
chicago: Hazak, Ora, Uri Obolski, Tomas Prat, Jiří Friml, Lilach Hadany, and Shaul
Yalovsky. “Bimodal Regulation of ICR1 Levels Generates Self-Organizing Auxin Distribution.”
PNAS. National Academy of Sciences, 2014. https://doi.org/10.1073/pnas.1413918111.
ieee: O. Hazak, U. Obolski, T. Prat, J. Friml, L. Hadany, and S. Yalovsky, “Bimodal
regulation of ICR1 levels generates self-organizing auxin distribution,” PNAS,
vol. 111, no. 50. National Academy of Sciences, pp. E5471–E5479, 2014.
ista: Hazak O, Obolski U, Prat T, Friml J, Hadany L, Yalovsky S. 2014. Bimodal regulation
of ICR1 levels generates self-organizing auxin distribution. PNAS. 111(50), E5471–E5479.
mla: Hazak, Ora, et al. “Bimodal Regulation of ICR1 Levels Generates Self-Organizing
Auxin Distribution.” PNAS, vol. 111, no. 50, National Academy of Sciences,
2014, pp. E5471–79, doi:10.1073/pnas.1413918111.
short: O. Hazak, U. Obolski, T. Prat, J. Friml, L. Hadany, S. Yalovsky, PNAS 111
(2014) E5471–E5479.
date_created: 2018-12-11T11:55:07Z
date_published: 2014-12-16T00:00:00Z
date_updated: 2025-09-29T12:06:13Z
day: '16'
department:
- _id: JiFr
doi: 10.1073/pnas.1413918111
external_id:
isi:
- '000346366500020'
fulldoi: https://doi.org/10.1073/pnas.1413918111
intvolume: ' 111'
isi: 1
issue: '50'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273421/
month: '12'
oa: 1
oa_version: Submitted Version
page: E5471 - E5479
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '5083'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Bimodal regulation of ICR1 levels generates self-organizing auxin distribution
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 111
year: '2014'
...
---
_id: '2061'
abstract:
- lang: eng
text: 'Development of cambium and its activity is important for our knowledge of
the mechanism of secondary growth. Arabidopsis thaliana emerges as a good model
plant for such a kind of study. Thus, this paper reports on cellular events taking
place in the interfascicular regions of inflorescence stems of A. thaliana, leading
to the development of interfascicular cambium from differentiated interfascicular
parenchyma cells (IPC). These events are as follows: appearance of auxin accumulation,
PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane
and periclinal divisions. Distribution of auxin was observed to be higher in differentiating
into cambium parenchyma cells compared to cells within the pith and cortex. Expression
of PIN1 in IPC was always preceded by auxin accumulation. Basal localization of
PIN1 was already established in the cells prior to their periclinal division.
These cellular events initiated within parenchyma cells adjacent to the vascular
bundles and successively extended from that point towards the middle region of
the interfascicular area, located between neighboring vascular bundles. The final
consequence of which was the closure of the cambial ring within the stem. Changes
in the chemical composition of IPC walls were also detected and included changes
of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs).
In summary, results presented in this paper describe interfascicular cambium ontogenesis
in terms of successive cellular events in the interfascicular regions of inflorescence
stems of Arabidopsis.'
article_processing_charge: No
author:
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Ewa
full_name: Kurczyñska, Ewa
last_name: Kurczyñska
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mazur E, Kurczyñska E, Friml J. Cellular events during interfascicular cambium
ontogenesis in inflorescence stems of Arabidopsis. Protoplasma. 2014;251(5):1125-1139.
doi:10.1007/s00709-014-0620-5
apa: Mazur, E., Kurczyñska, E., & Friml, J. (2014). Cellular events during interfascicular
cambium ontogenesis in inflorescence stems of Arabidopsis. Protoplasma.
Springer. https://doi.org/10.1007/s00709-014-0620-5
chicago: Mazur, Ewa, Ewa Kurczyñska, and Jiří Friml. “Cellular Events during Interfascicular
Cambium Ontogenesis in Inflorescence Stems of Arabidopsis.” Protoplasma.
Springer, 2014. https://doi.org/10.1007/s00709-014-0620-5.
ieee: E. Mazur, E. Kurczyñska, and J. Friml, “Cellular events during interfascicular
cambium ontogenesis in inflorescence stems of Arabidopsis,” Protoplasma,
vol. 251, no. 5. Springer, pp. 1125–1139, 2014.
ista: Mazur E, Kurczyñska E, Friml J. 2014. Cellular events during interfascicular
cambium ontogenesis in inflorescence stems of Arabidopsis. Protoplasma. 251(5),
1125–1139.
mla: Mazur, Ewa, et al. “Cellular Events during Interfascicular Cambium Ontogenesis
in Inflorescence Stems of Arabidopsis.” Protoplasma, vol. 251, no. 5, Springer,
2014, pp. 1125–39, doi:10.1007/s00709-014-0620-5.
short: E. Mazur, E. Kurczyñska, J. Friml, Protoplasma 251 (2014) 1125–1139.
date_created: 2018-12-11T11:55:29Z
date_published: 2014-02-14T00:00:00Z
date_updated: 2025-09-29T11:48:32Z
day: '14'
department:
- _id: JiFr
doi: 10.1007/s00709-014-0620-5
external_id:
isi:
- '000340480300012'
fulldoi: https://doi.org/10.1007/s00709-014-0620-5
intvolume: ' 251'
isi: 1
issue: '5'
language:
- iso: eng
month: '02'
oa_version: None
page: 1125 - 1139
publication: Protoplasma
publication_status: published
publisher: Springer
publist_id: '4985'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cellular events during interfascicular cambium ontogenesis in inflorescence
stems of Arabidopsis
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 251
year: '2014'
...
---
_id: '1806'
abstract:
- lang: eng
text: The generation of asymmetry, at both cellular and tissue level, is one of
the most essential capabilities of all eukaryotic organisms. It mediates basically
all multicellular development ranging from embryogenesis and de novo organ formation
till responses to various environmental stimuli. In plants, the awe-inspiring
number of such processes is regulated by phytohormone auxin and its directional,
cell-to-cell transport. The mediators of this transport, PIN auxin transporters,
are asymmetrically localized at the plasma membrane, and this polar localization
determines the directionality of intercellular auxin flow. Thus, auxin transport
contributes crucially to the generation of local auxin gradients or maxima, which
instruct given cell to change its developmental program. Here, we introduce and
discuss the molecular components and cellular mechanisms regulating the generation
and maintenance of cellular PIN polarity, as the general hallmarks of cell polarity
in plants.
author:
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Baster P, Friml J. Auxin on the road navigated by cellular PIN polarity. In:
Zažímalová E, Petrášek J, Benková E, eds. Auxin and Its Role in Plant Development.
Springer; 2014:143-170. doi:10.1007/978-3-7091-1526-8_8'
apa: Baster, P., & Friml, J. (2014). Auxin on the road navigated by cellular
PIN polarity. In E. Zažímalová, J. Petrášek, & E. Benková (Eds.), Auxin
and Its Role in Plant Development (pp. 143–170). Springer. https://doi.org/10.1007/978-3-7091-1526-8_8
chicago: Baster, Pawel, and Jiří Friml. “Auxin on the Road Navigated by Cellular
PIN Polarity.” In Auxin and Its Role in Plant Development, edited by Eva
Zažímalová, Jan Petrášek, and Eva Benková, 143–70. Springer, 2014. https://doi.org/10.1007/978-3-7091-1526-8_8.
ieee: P. Baster and J. Friml, “Auxin on the road navigated by cellular PIN polarity,”
in Auxin and Its Role in Plant Development, E. Zažímalová, J. Petrášek,
and E. Benková, Eds. Springer, 2014, pp. 143–170.
ista: 'Baster P, Friml J. 2014.Auxin on the road navigated by cellular PIN polarity.
In: Auxin and Its Role in Plant Development. , 143–170.'
mla: Baster, Pawel, and Jiří Friml. “Auxin on the Road Navigated by Cellular PIN
Polarity.” Auxin and Its Role in Plant Development, edited by Eva Zažímalová
et al., Springer, 2014, pp. 143–70, doi:10.1007/978-3-7091-1526-8_8.
short: P. Baster, J. Friml, in:, E. Zažímalová, J. Petrášek, E. Benková (Eds.),
Auxin and Its Role in Plant Development, Springer, 2014, pp. 143–170.
corr_author: '1'
date_created: 2018-12-11T11:54:07Z
date_published: 2014-04-01T00:00:00Z
date_updated: 2024-10-09T20:55:54Z
day: '01'
department:
- _id: JiFr
doi: 10.1007/978-3-7091-1526-8_8
editor:
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
fulldoi: https://doi.org/10.1007/978-3-7091-1526-8_8
language:
- iso: eng
month: '04'
oa_version: None
page: 143 - 170
publication: Auxin and Its Role in Plant Development
publication_status: published
publisher: Springer
publist_id: '5304'
quality_controlled: '1'
scopus_import: 1
status: public
title: Auxin on the road navigated by cellular PIN polarity
type: book_chapter
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
year: '2014'
...
---
OA_place: publisher
_id: '1402'
abstract:
- lang: eng
text: Phosphatidylinositol (Ptdlns) is a structural phospholipid that can be phosphorylated
into various lipid signaling molecules, designated polyphosphoinositides (PPIs).
The reversible phosphorylation of PPIs on the 3, 4, or 5 position of inositol
is performed by a set of organelle-specific kinases and phosphatases, and the
characteristic head groups make these molecules ideal for regulating biological
processes in time and space. In yeast and mammals, Ptdlns3P and Ptdlns(3,5)P2
play crucial roles in trafficking toward the lytic compartments, whereas the role
in plants is not yet fully understood. Here we identified the role of a land plant-specific
subgroup of PPI phosphatases, the suppressor of actin 2 (SAC2) to SAC5, during
vauolar trafficking and morphogenesis in Arabidopsis thaliana. SAC2-SAC5 localize
to the tonoplast along with Ptdlns3P, the presumable product of their activity.
in SAC gain- and loss-of-function mutants, the levels of Ptdlns monophosphates
and bisphosphates were changed, with opposite effects on the morphology of storage
and lytic vacuoles, and the trafficking toward the vacuoles was defective. Moreover,
multiple sac knockout mutants had an increased number of smaller storage and lytic
vacuoles, whereas extralarge vacuoles were observed in the overexpression lines,
correlating with various growth and developmental defects. The fragmented vacuolar
phenotype of sac mutants could be mimicked by treating wild-type seedlings with
Ptdlns(3,5)P2, corroborating that this PPI is important for vacuole morphology.
Taken together, these results provide evidence that PPIs, together with their
metabolic enzymes SAC2-SAC5, are crucial for vacuolar trafficking and for vacuolar
morphology and function in plants.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
citation:
ama: Marhavá P. Molecular mechanisms of patterning and subcellular trafficking in
Arabidopsis thaliana. 2014.
apa: Marhavá, P. (2014). Molecular mechanisms of patterning and subcellular trafficking
in Arabidopsis thaliana. Institute of Science and Technology Austria.
chicago: Marhavá, Petra. “Molecular Mechanisms of Patterning and Subcellular Trafficking
in Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2014.
ieee: P. Marhavá, “Molecular mechanisms of patterning and subcellular trafficking
in Arabidopsis thaliana,” Institute of Science and Technology Austria, 2014.
ista: Marhavá P. 2014. Molecular mechanisms of patterning and subcellular trafficking
in Arabidopsis thaliana. Institute of Science and Technology Austria.
mla: Marhavá, Petra. Molecular Mechanisms of Patterning and Subcellular Trafficking
in Arabidopsis Thaliana. Institute of Science and Technology Austria, 2014.
short: P. Marhavá, Molecular Mechanisms of Patterning and Subcellular Trafficking
in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2014.
corr_author: '1'
date_created: 2018-12-11T11:51:49Z
date_published: 2014-12-01T00:00:00Z
date_updated: 2026-07-29T10:06:44Z
day: '01'
degree_awarded: PhD
department:
- _id: JiFr
- _id: GradSch
doi_confirm: '1'
language:
- iso: eng
month: '12'
oa_version: None
page: '90'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '5805'
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis
thaliana
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2014'
...
---
_id: '10895'
abstract:
- lang: eng
text: 'Due to their sessile lifestyles, plants need to deal with the limitations
and stresses imposed by the changing environment. Plants cope with these by a
remarkable developmental flexibility, which is embedded in their strategy to survive.
Plants can adjust their size, shape and number of organs, bend according to gravity
and light, and regenerate tissues that were damaged, utilizing a coordinating,
intercellular signal, the plant hormone, auxin. Another versatile signal is the
cation, Ca2+, which is a crucial second messenger for many rapid cellular processes
during responses to a wide range of endogenous and environmental signals, such
as hormones, light, drought stress and others. Auxin is a good candidate for one
of these Ca2+-activating signals. However, the role of auxin-induced Ca2+ signaling
is poorly understood. Here, we will provide an overview of possible developmental
and physiological roles, as well as mechanisms underlying the interconnection
of Ca2+ and auxin signaling. '
article_processing_charge: No
article_type: original
author:
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Vanneste S, Friml J. Calcium: The missing link in auxin action. Plants.
2013;2(4):650-675. doi:10.3390/plants2040650'
apa: 'Vanneste, S., & Friml, J. (2013). Calcium: The missing link in auxin action.
Plants. MDPI. https://doi.org/10.3390/plants2040650'
chicago: 'Vanneste, Steffen, and Jiří Friml. “Calcium: The Missing Link in Auxin
Action.” Plants. MDPI, 2013. https://doi.org/10.3390/plants2040650.'
ieee: 'S. Vanneste and J. Friml, “Calcium: The missing link in auxin action,” Plants,
vol. 2, no. 4. MDPI, pp. 650–675, 2013.'
ista: 'Vanneste S, Friml J. 2013. Calcium: The missing link in auxin action. Plants.
2(4), 650–675.'
mla: 'Vanneste, Steffen, and Jiří Friml. “Calcium: The Missing Link in Auxin Action.”
Plants, vol. 2, no. 4, MDPI, 2013, pp. 650–75, doi:10.3390/plants2040650.'
short: S. Vanneste, J. Friml, Plants 2 (2013) 650–675.
corr_author: '1'
date_created: 2022-03-21T07:13:49Z
date_published: 2013-10-21T00:00:00Z
date_updated: 2024-10-09T21:01:52Z
day: '21'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/plants2040650
external_id:
pmid:
- '27137397'
file:
- access_level: open_access
checksum: fb4ff2e820e344e253c9197544610be6
content_type: application/pdf
creator: dernst
date_created: 2022-03-21T12:12:56Z
date_updated: 2022-03-21T12:12:56Z
file_id: '10916'
file_name: 2013_Plants_Vanneste.pdf
file_size: 670188
relation: main_file
success: 1
file_date_updated: 2022-03-21T12:12:56Z
fulldoi: https://doi.org/10.3390/plants2040650
has_accepted_license: '1'
intvolume: ' 2'
issue: '4'
keyword:
- Plant Science
- Ecology
- Ecology
- Evolution
- Behavior and Systematics
language:
- iso: eng
license: https://creativecommons.org/licenses/by/3.0/
month: '10'
oa: 1
oa_version: Published Version
page: 650-675
pmid: 1
publication: Plants
publication_identifier:
issn:
- 2223-7747
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Calcium: The missing link in auxin action'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/3.0/legalcode
name: Creative Commons Attribution 3.0 Unported (CC BY 3.0)
short: CC BY (3.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2
year: '2013'
...
---
_id: '2290'
abstract:
- lang: eng
text: The plant hormone indole-acetic acid (auxin) is essential for many aspects
of plant development. Auxin-mediated growth regulation typically involves the
establishment of an auxin concentration gradient mediated by polarly localized
auxin transporters. The localization of auxin carriers and their amount at the
plasma membrane are controlled by membrane trafficking processes such as secretion,
endocytosis, and recycling. In contrast to endocytosis or recycling, how the secretory
pathway mediates the localization of auxin carriers is not well understood. In
this study we have used the differential cell elongation process during apical
hook development to elucidate the mechanisms underlying the post-Golgi trafficking
of auxin carriers in Arabidopsis. We show that differential cell elongation during
apical hook development is defective in Arabidopsis mutant echidna (ech). ECH
protein is required for the trans-Golgi network (TGN)-mediated trafficking of
the auxin influx carrier AUX1 to the plasma membrane. In contrast, ech mutation
only marginally perturbs the trafficking of the highly related auxin influx carrier
LIKE-AUX1-3 or the auxin efflux carrier PIN-FORMED-3, both also involved in hook
development. Electron tomography reveals that the trafficking defects in ech mutant
are associated with the perturbation of secretory vesicle genesis from the TGN.
Our results identify differential mechanisms for the post-Golgi trafficking of
de novo-synthesized auxin carriers to plasma membrane from the TGN and reveal
how trafficking of auxin influx carriers mediates the control of differential
cell elongation in apical hook development.
article_processing_charge: No
author:
- first_name: Yohann
full_name: Boutté, Yohann
last_name: Boutté
- first_name: Kristoffer
full_name: Jonsson, Kristoffer
last_name: Jonsson
- first_name: Heather
full_name: Mcfarlane, Heather
last_name: Mcfarlane
- first_name: Errin
full_name: Johnson, Errin
last_name: Johnson
- first_name: Delphine
full_name: Gendre, Delphine
last_name: Gendre
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Lacey
full_name: Samuels, Lacey
last_name: Samuels
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Rishikesh
full_name: Bhalerao, Rishikesh
last_name: Bhalerao
citation:
ama: Boutté Y, Jonsson K, Mcfarlane H, et al. ECHIDNA mediated post Golgi trafficking
of auxin carriers for differential cell elongation. PNAS. 2013;110(40):16259-16264.
doi:10.1073/pnas.1309057110
apa: Boutté, Y., Jonsson, K., Mcfarlane, H., Johnson, E., Gendre, D., Swarup, R.,
… Bhalerao, R. (2013). ECHIDNA mediated post Golgi trafficking of auxin carriers
for differential cell elongation. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1309057110
chicago: Boutté, Yohann, Kristoffer Jonsson, Heather Mcfarlane, Errin Johnson, Delphine
Gendre, Ranjan Swarup, Jiří Friml, Lacey Samuels, Stéphanie Robert, and Rishikesh
Bhalerao. “ECHIDNA Mediated Post Golgi Trafficking of Auxin Carriers for Differential
Cell Elongation.” PNAS. National Academy of Sciences, 2013. https://doi.org/10.1073/pnas.1309057110.
ieee: Y. Boutté et al., “ECHIDNA mediated post Golgi trafficking of auxin
carriers for differential cell elongation,” PNAS, vol. 110, no. 40. National
Academy of Sciences, pp. 16259–16264, 2013.
ista: Boutté Y, Jonsson K, Mcfarlane H, Johnson E, Gendre D, Swarup R, Friml J,
Samuels L, Robert S, Bhalerao R. 2013. ECHIDNA mediated post Golgi trafficking
of auxin carriers for differential cell elongation. PNAS. 110(40), 16259–16264.
mla: Boutté, Yohann, et al. “ECHIDNA Mediated Post Golgi Trafficking of Auxin Carriers
for Differential Cell Elongation.” PNAS, vol. 110, no. 40, National Academy
of Sciences, 2013, pp. 16259–64, doi:10.1073/pnas.1309057110.
short: Y. Boutté, K. Jonsson, H. Mcfarlane, E. Johnson, D. Gendre, R. Swarup, J.
Friml, L. Samuels, S. Robert, R. Bhalerao, PNAS 110 (2013) 16259–16264.
date_created: 2018-12-11T11:56:48Z
date_published: 2013-10-01T00:00:00Z
date_updated: 2025-09-29T14:22:33Z
day: '01'
department:
- _id: JiFr
doi: 10.1073/pnas.1309057110
external_id:
isi:
- '000325105500090'
pmid:
- '24043780'
fulldoi: https://doi.org/10.1073/pnas.1309057110
intvolume: ' 110'
isi: 1
issue: '40'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791722/
month: '10'
oa: 1
oa_version: Submitted Version
page: 16259 - 16264
pmid: 1
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '4639'
quality_controlled: '1'
scopus_import: '1'
status: public
title: ECHIDNA mediated post Golgi trafficking of auxin carriers for differential
cell elongation
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 110
year: '2013'
...
---
_id: '2448'
abstract:
- lang: eng
text: Cell-to-cell directional flow of the phytohormone auxin is primarily established
by polar localization of the PIN auxin transporters, a process tightly regulated
at multiple levels by auxin itself. We recently reported that, in the context
of strong auxin flows, activity of the vacuolar ZIFL1.1 transporter is required
for fine-tuning of polar auxin transport rates in the Arabidopsis root. In particular,
ZIFL1.1 function protects plasma-membrane stability of the PIN2 carrier in epidermal
root tip cells under conditions normally triggering PIN2 degradation. Here, we
show that ZIFL1.1 activity at the root tip also promotes PIN1 plasma-membrane
abundance in central cylinder cells, thus supporting the notion that ZIFL1.1 acts
as a general positive modulator of polar auxin transport in roots.
article_number: e25688
article_processing_charge: No
article_type: original
author:
- first_name: Estelle
full_name: Remy, Estelle
last_name: Remy
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Paula
full_name: Duque, Paula
last_name: Duque
citation:
ama: Remy E, Baster P, Friml J, Duque P. ZIFL1.1 transporter modulates polar auxin
transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root
tip. Plant Signaling & Behavior. 2013;8(10). doi:10.4161/psb.25688
apa: Remy, E., Baster, P., Friml, J., & Duque, P. (2013). ZIFL1.1 transporter
modulates polar auxin transport by stabilizing membrane abundance of multiple
PINs in Arabidopsis root tip. Plant Signaling & Behavior. Taylor &
Francis. https://doi.org/10.4161/psb.25688
chicago: Remy, Estelle, Pawel Baster, Jiří Friml, and Paula Duque. “ZIFL1.1 Transporter
Modulates Polar Auxin Transport by Stabilizing Membrane Abundance of Multiple
PINs in Arabidopsis Root Tip.” Plant Signaling & Behavior. Taylor &
Francis, 2013. https://doi.org/10.4161/psb.25688.
ieee: E. Remy, P. Baster, J. Friml, and P. Duque, “ZIFL1.1 transporter modulates
polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis
root tip,” Plant Signaling & Behavior, vol. 8, no. 10. Taylor &
Francis, 2013.
ista: Remy E, Baster P, Friml J, Duque P. 2013. ZIFL1.1 transporter modulates polar
auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis
root tip. Plant Signaling & Behavior. 8(10), e25688.
mla: Remy, Estelle, et al. “ZIFL1.1 Transporter Modulates Polar Auxin Transport
by Stabilizing Membrane Abundance of Multiple PINs in Arabidopsis Root Tip.” Plant
Signaling & Behavior, vol. 8, no. 10, e25688, Taylor & Francis, 2013,
doi:10.4161/psb.25688.
short: E. Remy, P. Baster, J. Friml, P. Duque, Plant Signaling & Behavior 8
(2013).
date_created: 2018-12-11T11:57:43Z
date_published: 2013-07-10T00:00:00Z
date_updated: 2025-04-15T07:48:02Z
day: '10'
department:
- _id: JiFr
doi: 10.4161/psb.25688
ec_funded: 1
external_id:
pmid:
- '23857365'
fulldoi: https://doi.org/10.4161/psb.25688
intvolume: ' 8'
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091088/
month: '07'
oa: 1
oa_version: Submitted Version
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Plant Signaling & Behavior
publication_status: published
publisher: Taylor & Francis
publist_id: '4455'
quality_controlled: '1'
scopus_import: '1'
status: public
title: ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane
abundance of multiple PINs in Arabidopsis root tip
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2013'
...
---
_id: '2449'
abstract:
- lang: eng
text: 'Intracellular protein routing is mediated by vesicular transport which is
tightly regulated in eukaryotes. The protein and lipid homeostasis depends on
coordinated delivery of de novo synthesized or recycled cargoes to the plasma
membrane by exocytosis and their subsequent removal by rerouting them for recycling
or degradation. Here, we report the characterization of protein affected trafficking
3 (pat3) mutant that we identified by an epifluorescence-based forward genetic
screen for mutants defective in subcellular distribution of Arabidopsis auxin
transporter PIN1–GFP. While pat3 displays largely normal plant morphology and
development in nutrient-rich conditions, it shows strong ectopic intracellular
accumulations of different plasma membrane cargoes in structures that resemble
prevacuolar compartments (PVC) with an aberrant morphology. Genetic mapping revealed
that pat3 is defective in vacuolar protein sorting 35A (VPS35A), a putative subunit
of the retromer complex that mediates retrograde trafficking between the PVC and
trans-Golgi network. Similarly, a mutant defective in another retromer subunit,
vps29, shows comparable subcellular defects in PVC morphology and protein accumulation.
Thus, our data provide evidence that the retromer components VPS35A and VPS29
are essential for normal PVC morphology and normal trafficking of plasma membrane
proteins in plants. In addition, we show that, out of the three VPS35 retromer
subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing
role in trafficking to the lytic vacuole, presenting another level of complexity
in the retromer-dependent vacuolar sorting. '
article_processing_charge: No
author:
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Murguel
full_name: Feraru, Murguel
last_name: Feraru
- first_name: Sibylle
full_name: Hirsch, Sibylle
last_name: Hirsch
- first_name: Riet
full_name: De Rycke, Riet
last_name: De Rycke
- first_name: Claudiu
full_name: Nicuales, Claudiu
last_name: Nicuales
- first_name: Jelle
full_name: Van Leene, Jelle
last_name: Van Leene
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Nodzyński T, Feraru M, Hirsch S, et al. Retromer subunits VPS35A and VPS29
mediate prevacuolar compartment (PVC) function in Arabidopsis. Molecular Plant.
2013;6(6):1849-1862. doi:10.1093/mp/sst044
apa: Nodzyński, T., Feraru, M., Hirsch, S., De Rycke, R., Nicuales, C., Van Leene,
J., … Friml, J. (2013). Retromer subunits VPS35A and VPS29 mediate prevacuolar
compartment (PVC) function in Arabidopsis. Molecular Plant. Cell Press.
https://doi.org/10.1093/mp/sst044
chicago: Nodzyński, Tomasz, Murguel Feraru, Sibylle Hirsch, Riet De Rycke, Claudiu
Nicuales, Jelle Van Leene, Geert De Jaeger, Steffen Vanneste, and Jiří Friml.
“Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function
in Arabidopsis.” Molecular Plant. Cell Press, 2013. https://doi.org/10.1093/mp/sst044.
ieee: T. Nodzyński et al., “Retromer subunits VPS35A and VPS29 mediate prevacuolar
compartment (PVC) function in Arabidopsis,” Molecular Plant, vol. 6, no.
6. Cell Press, pp. 1849–1862, 2013.
ista: Nodzyński T, Feraru M, Hirsch S, De Rycke R, Nicuales C, Van Leene J, De Jaeger
G, Vanneste S, Friml J. 2013. Retromer subunits VPS35A and VPS29 mediate prevacuolar
compartment (PVC) function in Arabidopsis. Molecular Plant. 6(6), 1849–1862.
mla: Nodzyński, Tomasz, et al. “Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar
Compartment (PVC) Function in Arabidopsis.” Molecular Plant, vol. 6, no.
6, Cell Press, 2013, pp. 1849–62, doi:10.1093/mp/sst044.
short: T. Nodzyński, M. Feraru, S. Hirsch, R. De Rycke, C. Nicuales, J. Van Leene,
G. De Jaeger, S. Vanneste, J. Friml, Molecular Plant 6 (2013) 1849–1862.
corr_author: '1'
date_created: 2018-12-11T11:57:44Z
date_published: 2013-11-01T00:00:00Z
date_updated: 2025-09-29T14:14:22Z
day: '01'
department:
- _id: JiFr
doi: 10.1093/mp/sst044
external_id:
isi:
- '000327541200010'
fulldoi: https://doi.org/10.1093/mp/sst044
intvolume: ' 6'
isi: 1
issue: '6'
language:
- iso: eng
month: '11'
oa_version: None
page: 1849 - 1862
publication: Molecular Plant
publication_status: published
publisher: Cell Press
publist_id: '4454'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function
in Arabidopsis
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 6
year: '2013'
...
---
_id: '2470'
abstract:
- lang: eng
text: Background:Auxin binding protein 1 (ABP1) is a putative auxin receptor and
its function is indispensable for plant growth and development. ABP1 has been
shown to be involved in auxin-dependent regulation of cell division and expansion,
in plasma-membrane-related processes such as changes in transmembrane potential,
and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated
downstream pathway remains elusive.Methodology/Principal Findings:Using auxin
transport assays and quantitative analysis of cellular morphology we show that
ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can
counterbalance increased auxin efflux and auxin starvation phenotypes caused by
the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the
ABP1-controlled vesicle trafficking processes, including positive regulation of
endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery
after photobleaching (FRAP) and pharmacological manipulations.Conclusions/Significance:The
findings indicate the involvement of ABP1 in control of rate of auxin transport
across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity
at the plasma membrane, and highlighting the relevance of ABP1 for the formation
of developmentally important, PIN-dependent auxin gradients.
article_number: e70050
article_processing_charge: No
author:
- first_name: Milada
full_name: Čovanová, Milada
last_name: Čovanová
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jan
full_name: Rychtář, Jan
last_name: Rychtář
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
citation:
ama: Čovanová M, Sauer M, Rychtář J, Friml J, Petrášek J, Zažímalová E. Overexpression
of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco
cells. PLoS One. 2013;8(7). doi:10.1371/journal.pone.0070050
apa: Čovanová, M., Sauer, M., Rychtář, J., Friml, J., Petrášek, J., & Zažímalová,
E. (2013). Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent
auxin transport in tobacco cells. PLoS One. Public Library of Science.
https://doi.org/10.1371/journal.pone.0070050
chicago: Čovanová, Milada, Michael Sauer, Jan Rychtář, Jiří Friml, Jan Petrášek,
and Eva Zažímalová. “Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent
Auxin Transport in Tobacco Cells.” PLoS One. Public Library of Science,
2013. https://doi.org/10.1371/journal.pone.0070050.
ieee: M. Čovanová, M. Sauer, J. Rychtář, J. Friml, J. Petrášek, and E. Zažímalová,
“Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport
in tobacco cells,” PLoS One, vol. 8, no. 7. Public Library of Science,
2013.
ista: Čovanová M, Sauer M, Rychtář J, Friml J, Petrášek J, Zažímalová E. 2013. Overexpression
of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco
cells. PLoS One. 8(7), e70050.
mla: Čovanová, Milada, et al. “Overexpression of the Auxin Binding PROTEIN1 Modulates
PIN-Dependent Auxin Transport in Tobacco Cells.” PLoS One, vol. 8, no.
7, e70050, Public Library of Science, 2013, doi:10.1371/journal.pone.0070050.
short: M. Čovanová, M. Sauer, J. Rychtář, J. Friml, J. Petrášek, E. Zažímalová,
PLoS One 8 (2013).
date_created: 2018-12-11T11:57:51Z
date_published: 2013-07-23T00:00:00Z
date_updated: 2025-09-29T14:11:54Z
day: '23'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1371/journal.pone.0070050
external_id:
isi:
- '000325211000181'
file:
- access_level: open_access
checksum: 2d47ef47616ef4de1d517d146548184e
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:08:21Z
date_updated: 2020-07-14T12:45:41Z
file_id: '4681'
file_name: IST-2016-413-v1+1_journal.pone.0070050.pdf
file_size: 2294955
relation: main_file
file_date_updated: 2020-07-14T12:45:41Z
fulldoi: https://doi.org/10.1371/journal.pone.0070050
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '7'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
publication: PLoS One
publication_status: published
publisher: Public Library of Science
publist_id: '4432'
pubrep_id: '413'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin
transport in tobacco cells
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 8
year: '2013'
...
---
_id: '2472'
abstract:
- lang: eng
text: Plant-specific PIN-formed (PIN) efflux transporters for the plant hormone
auxin are required for tissue-specific directional auxin transport and cellular
auxin homeostasis. The Arabidopsis PIN protein family has been shown to play important
roles in developmental processes such as embryogenesis, organogenesis, vascular
tissue differentiation, root meristem patterning and tropic growth. Here we analyzed
roles of the less characterised Arabidopsis PIN6 auxin transporter. PIN6 is auxin-inducible
and is expressed during multiple auxin-regulated developmental processes. Loss
of pin6 function interfered with primary root growth and lateral root development.
Misexpression of PIN6 affected auxin transport and interfered with auxin homeostasis
in other growth processes such as shoot apical dominance, lateral root primordia
development, adventitious root formation, root hair outgrowth and root waving.
These changes in auxin-regulated growth correlated with a reduction in total auxin
transport as well as with an altered activity of DR5-GUS auxin response reporter.
Overall, the data indicate that PIN6 regulates auxin homeostasis during plant
development.
article_number: e70069
article_processing_charge: No
author:
- first_name: Christopher
full_name: Cazzonelli, Christopher
last_name: Cazzonelli
- first_name: Marleen
full_name: Vanstraelen, Marleen
last_name: Vanstraelen
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Kuide
full_name: Yin, Kuide
last_name: Yin
- first_name: Ashley
full_name: Carron Arthur, Ashley
last_name: Carron Arthur
- first_name: Nazia
full_name: Nisar, Nazia
last_name: Nisar
- first_name: Gauri
full_name: Tarle, Gauri
last_name: Tarle
- first_name: Abby
full_name: Cuttriss, Abby
last_name: Cuttriss
- first_name: Iain
full_name: Searle, Iain
last_name: Searle
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Ulrike
full_name: Mathesius, Ulrike
last_name: Mathesius
- first_name: Josette
full_name: Masle, Josette
last_name: Masle
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Barry
full_name: Pogson, Barry
last_name: Pogson
citation:
ama: Cazzonelli C, Vanstraelen M, Simon S, et al. Role of the Arabidopsis PIN6 auxin
transporter in auxin homeostasis and auxin-mediated development. PLoS One.
2013;8(7). doi:10.1371/journal.pone.0070069
apa: Cazzonelli, C., Vanstraelen, M., Simon, S., Yin, K., Carron Arthur, A., Nisar,
N., … Pogson, B. (2013). Role of the Arabidopsis PIN6 auxin transporter in auxin
homeostasis and auxin-mediated development. PLoS One. Public Library of
Science. https://doi.org/10.1371/journal.pone.0070069
chicago: Cazzonelli, Christopher, Marleen Vanstraelen, Sibu Simon, Kuide Yin, Ashley
Carron Arthur, Nazia Nisar, Gauri Tarle, et al. “Role of the Arabidopsis PIN6
Auxin Transporter in Auxin Homeostasis and Auxin-Mediated Development.” PLoS
One. Public Library of Science, 2013. https://doi.org/10.1371/journal.pone.0070069.
ieee: C. Cazzonelli et al., “Role of the Arabidopsis PIN6 auxin transporter
in auxin homeostasis and auxin-mediated development,” PLoS One, vol. 8,
no. 7. Public Library of Science, 2013.
ista: Cazzonelli C, Vanstraelen M, Simon S, Yin K, Carron Arthur A, Nisar N, Tarle
G, Cuttriss A, Searle I, Benková E, Mathesius U, Masle J, Friml J, Pogson B. 2013.
Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated
development. PLoS One. 8(7), e70069.
mla: Cazzonelli, Christopher, et al. “Role of the Arabidopsis PIN6 Auxin Transporter
in Auxin Homeostasis and Auxin-Mediated Development.” PLoS One, vol. 8,
no. 7, e70069, Public Library of Science, 2013, doi:10.1371/journal.pone.0070069.
short: C. Cazzonelli, M. Vanstraelen, S. Simon, K. Yin, A. Carron Arthur, N. Nisar,
G. Tarle, A. Cuttriss, I. Searle, E. Benková, U. Mathesius, J. Masle, J. Friml,
B. Pogson, PLoS One 8 (2013).
date_created: 2018-12-11T11:57:52Z
date_published: 2013-07-29T00:00:00Z
date_updated: 2025-09-29T14:11:25Z
day: '29'
ddc:
- '580'
- '570'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1371/journal.pone.0070069
ec_funded: 1
external_id:
isi:
- '000323369700128'
file:
- access_level: open_access
checksum: 3be71828b6c2ba9c90eb7056e3f7f57a
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:34Z
date_updated: 2020-07-14T12:45:41Z
file_id: '5222'
file_name: IST-2015-393-v1+1_journal.pone.0070069.pdf
file_size: 9003465
relation: main_file
file_date_updated: 2020-07-14T12:45:41Z
fulldoi: https://doi.org/10.1371/journal.pone.0070069
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '7'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PLoS One
publication_status: published
publisher: Public Library of Science
publist_id: '4431'
pubrep_id: '393'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated
development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 8
year: '2013'
...
---
_id: '2808'
abstract:
- lang: eng
text: In order to establish a reference for analysis of the function of auxin and
the auxin biosynthesis regulators SHORT INTERNODE/ STYLISH (SHI/STY) during Physcomitrella
patens reproductive development, we have described male (antheridial) and female
(archegonial) development in detail, including temporal and positional information
of organ initiation. This has allowed us to define discrete stages of organ morphogenesis
and to show that reproductive organ development in P. patens is highly organized
and that organ phyllotaxis differs between vegetative and reproductive development.
Using the PpSHI1 and PpSHI2 reporter and knockout lines, the auxin reporters GmGH3pro:GUS
and PpPINApro:GFP-GUS, and the auxin-conjugating transgene PpSHI2pro:IAAL, we
could show that the PpSHI genes, and by inference also auxin, play important roles
for reproductive organ development in moss. The PpSHI genes are required for the
apical opening of the reproductive organs, the final differentiation of the egg
cell, and the progression of canal cells into a cell death program. The apical
cells of the archegonium, the canal cells, and the egg cell are also sites of
auxin responsiveness and are affected by reduced levels of active auxin, suggesting
that auxin mediates PpSHI function in the reproductive organs.
article_processing_charge: No
author:
- first_name: Katarina
full_name: Landberg, Katarina
last_name: Landberg
- first_name: Eric
full_name: Pederson, Eric
last_name: Pederson
- first_name: Tom
full_name: Viaene, Tom
last_name: Viaene
- first_name: Behruz
full_name: Bozorg, Behruz
last_name: Bozorg
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Henrik
full_name: Jönsson, Henrik
last_name: Jönsson
- first_name: Mattias
full_name: Thelander, Mattias
last_name: Thelander
- first_name: Eva
full_name: Sundberg, Eva
last_name: Sundberg
citation:
ama: Landberg K, Pederson E, Viaene T, et al. The moss physcomitrella patens reproductive
organ development is highly organized, affected by the two SHI/STY genes and by
the level of active auxin in the SHI/STY expression domain. Plant Physiology.
2013;162(3):1406-1419. doi:10.1104/pp.113.214023
apa: Landberg, K., Pederson, E., Viaene, T., Bozorg, B., Friml, J., Jönsson, H.,
… Sundberg, E. (2013). The moss physcomitrella patens reproductive organ development
is highly organized, affected by the two SHI/STY genes and by the level of active
auxin in the SHI/STY expression domain. Plant Physiology. American Society
of Plant Biologists. https://doi.org/10.1104/pp.113.214023
chicago: Landberg, Katarina, Eric Pederson, Tom Viaene, Behruz Bozorg, Jiří Friml,
Henrik Jönsson, Mattias Thelander, and Eva Sundberg. “The Moss Physcomitrella
Patens Reproductive Organ Development Is Highly Organized, Affected by the Two
SHI/STY Genes and by the Level of Active Auxin in the SHI/STY Expression Domain.”
Plant Physiology. American Society of Plant Biologists, 2013. https://doi.org/10.1104/pp.113.214023.
ieee: K. Landberg et al., “The moss physcomitrella patens reproductive organ
development is highly organized, affected by the two SHI/STY genes and by the
level of active auxin in the SHI/STY expression domain,” Plant Physiology,
vol. 162, no. 3. American Society of Plant Biologists, pp. 1406–1419, 2013.
ista: Landberg K, Pederson E, Viaene T, Bozorg B, Friml J, Jönsson H, Thelander
M, Sundberg E. 2013. The moss physcomitrella patens reproductive organ development
is highly organized, affected by the two SHI/STY genes and by the level of active
auxin in the SHI/STY expression domain. Plant Physiology. 162(3), 1406–1419.
mla: Landberg, Katarina, et al. “The Moss Physcomitrella Patens Reproductive Organ
Development Is Highly Organized, Affected by the Two SHI/STY Genes and by the
Level of Active Auxin in the SHI/STY Expression Domain.” Plant Physiology,
vol. 162, no. 3, American Society of Plant Biologists, 2013, pp. 1406–19, doi:10.1104/pp.113.214023.
short: K. Landberg, E. Pederson, T. Viaene, B. Bozorg, J. Friml, H. Jönsson, M.
Thelander, E. Sundberg, Plant Physiology 162 (2013) 1406–1419.
date_created: 2018-12-11T11:59:42Z
date_published: 2013-07-03T00:00:00Z
date_updated: 2025-09-29T14:06:13Z
day: '03'
department:
- _id: JiFr
doi: 10.1104/pp.113.214023
external_id:
isi:
- '000321325700015'
pmid:
- '23669745'
fulldoi: https://doi.org/10.1104/pp.113.214023
intvolume: ' 162'
isi: 1
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707547/
month: '07'
oa: 1
oa_version: Submitted Version
page: 1406 - 1419
pmid: 1
publication: Plant Physiology
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '4079'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The moss physcomitrella patens reproductive organ development is highly organized,
affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY
expression domain
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 162
year: '2013'
...
---
_id: '2821'
abstract:
- lang: eng
text: Many key aspects of plant development are regulated by the polarized transport
of the phytohormone auxin. Cellular auxin efflux, the rate-limiting step in this
process, has been shown to rely on the coordinated action of PIN-formed (PIN)
and B-type ATP binding cassette (ABCB) carriers. Here, we report that polar auxin
transport in the Arabidopsis thaliana root also requires the action of a Major
Facilitator Superfamily (MFS) transporter, Zinc-Induced Facilitator-Like 1 (ZIFL1).
Sequencing, promoter-reporter, and fluorescent protein fusion experiments indicate
that the full-length ZIFL1.1 protein and a truncated splice isoform, ZIFL1.3,
localize to the tonoplast of root cells and the plasma membrane of leaf stomatal
guard cells, respectively. Using reverse genetics, we show that the ZIFL1.1 transporter
regulates various root auxin-related processes, while the ZIFL1.3 isoform mediates
drought tolerance by regulating stomatal closure. Auxin transport and immunolocalization
assays demonstrate that ZIFL1.1 indirectly modulates cellular auxin efflux during
shootward auxin transport at the root tip, likely by regulating plasma membrane
PIN2 abundance. Finally, heterologous expression in yeast revealed that ZIFL1.1
and ZIFL1.3 share H+-coupled K+ transport activity. Thus, by determining the subcellular
and tissue distribution of two isoforms, alternative splicing dictates a dual
function for the ZIFL1 transporter. We propose that this MFS carrier regulates
stomatal movements and polar auxin transport by modulating potassium and proton
fluxes in Arabidopsis cells.
article_processing_charge: No
author:
- first_name: Estelle
full_name: Remy, Estelle
last_name: Remy
- first_name: Tânia
full_name: Cabrito, Tânia
last_name: Cabrito
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Rita
full_name: Batista, Rita
last_name: Batista
- first_name: Miguel
full_name: Teixeira, Miguel
last_name: Teixeira
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Isabel
full_name: Sá Correia, Isabel
last_name: Sá Correia
- first_name: Paula
full_name: Duque, Paula
last_name: Duque
citation:
ama: Remy E, Cabrito T, Baster P, et al. A major facilitator superfamily transporter
plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis.
Plant Cell. 2013;25(3):901-926. doi:10.1105/tpc.113.110353
apa: Remy, E., Cabrito, T., Baster, P., Batista, R., Teixeira, M., Friml, J., …
Duque, P. (2013). A major facilitator superfamily transporter plays a dual role
in polar auxin transport and drought stress tolerance in Arabidopsis. Plant
Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.113.110353
chicago: Remy, Estelle, Tânia Cabrito, Pawel Baster, Rita Batista, Miguel Teixeira,
Jiří Friml, Isabel Sá Correia, and Paula Duque. “A Major Facilitator Superfamily
Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance
in Arabidopsis.” Plant Cell. American Society of Plant Biologists, 2013.
https://doi.org/10.1105/tpc.113.110353.
ieee: E. Remy et al., “A major facilitator superfamily transporter plays
a dual role in polar auxin transport and drought stress tolerance in Arabidopsis,”
Plant Cell, vol. 25, no. 3. American Society of Plant Biologists, pp. 901–926,
2013.
ista: Remy E, Cabrito T, Baster P, Batista R, Teixeira M, Friml J, Sá Correia I,
Duque P. 2013. A major facilitator superfamily transporter plays a dual role in
polar auxin transport and drought stress tolerance in Arabidopsis. Plant Cell.
25(3), 901–926.
mla: Remy, Estelle, et al. “A Major Facilitator Superfamily Transporter Plays a
Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis.”
Plant Cell, vol. 25, no. 3, American Society of Plant Biologists, 2013,
pp. 901–26, doi:10.1105/tpc.113.110353.
short: E. Remy, T. Cabrito, P. Baster, R. Batista, M. Teixeira, J. Friml, I. Sá
Correia, P. Duque, Plant Cell 25 (2013) 901–926.
date_created: 2018-12-11T11:59:46Z
date_published: 2013-04-24T00:00:00Z
date_updated: 2025-09-29T13:57:58Z
day: '24'
department:
- _id: JiFr
doi: 10.1105/tpc.113.110353
external_id:
isi:
- '000318157100012'
pmid:
- '23524662'
fulldoi: https://doi.org/10.1105/tpc.113.110353
intvolume: ' 25'
isi: 1
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634696/
month: '04'
oa: 1
oa_version: Submitted Version
page: 901 - 926
pmid: 1
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3980'
quality_controlled: '1'
scopus_import: '1'
status: public
title: A major facilitator superfamily transporter plays a dual role in polar auxin
transport and drought stress tolerance in Arabidopsis
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 25
year: '2013'
...
---
_id: '2827'
abstract:
- lang: eng
text: Removal of cargos from the cell surface via endocytosis is an efficient mechanism
to regulate activities of plasma membrane (PM)-resident proteins, such as receptors
or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally
associated with pathogen defense. Here, we describe an unanticipated effect of
SA on subcellular endocytic cycling of proteins. Both exogenous treatments and
endogenously enhanced SA levels repressed endocytosis of different PM proteins.
The SA effect on endocytosis did not involve transcription or known components
of the SA signaling pathway for transcriptional regulation. SA likely targets
an endocytic mechanism that involves the coat protein clathrin, because SA interfered
with the clathrin incidence at the PM and clathrin-deficient mutants were less
sensitive to the impact of SA on the auxin distribution and root bending during
the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis
of the FLAGELLIN SENSING2 (FLS2) receptor during pathogen responses. Our data
suggest that the established SA impact on transcription in plant immunity and
the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent
mechanisms by which SA regulates distinct aspects of plant physiology.
article_processing_charge: No
author:
- first_name: Yunlong
full_name: Du, Yunlong
last_name: Du
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Martina
full_name: Beck, Martina
last_name: Beck
- first_name: Ellie
full_name: Himschoot, Ellie
last_name: Himschoot
- first_name: Hongjiang
full_name: Li, Hongjiang
id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0001-5039-9660
- first_name: Silke
full_name: Robatzek, Silke
last_name: Robatzek
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Du Y, Tejos R, Beck M, et al. Salicylic acid interferes with clathrin-mediated
endocytic protein trafficking. PNAS. 2013;110(19):7946-7951. doi:10.1073/pnas.1220205110
apa: Du, Y., Tejos, R., Beck, M., Himschoot, E., Li, H., Robatzek, S., … Friml,
J. (2013). Salicylic acid interferes with clathrin-mediated endocytic protein
trafficking. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1220205110
chicago: Du, Yunlong, Ricardo Tejos, Martina Beck, Ellie Himschoot, Hongjiang Li,
Silke Robatzek, Steffen Vanneste, and Jiří Friml. “Salicylic Acid Interferes with
Clathrin-Mediated Endocytic Protein Trafficking.” PNAS. National Academy
of Sciences, 2013. https://doi.org/10.1073/pnas.1220205110.
ieee: Y. Du et al., “Salicylic acid interferes with clathrin-mediated endocytic
protein trafficking,” PNAS, vol. 110, no. 19. National Academy of Sciences,
pp. 7946–7951, 2013.
ista: Du Y, Tejos R, Beck M, Himschoot E, Li H, Robatzek S, Vanneste S, Friml J.
2013. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking.
PNAS. 110(19), 7946–7951.
mla: Du, Yunlong, et al. “Salicylic Acid Interferes with Clathrin-Mediated Endocytic
Protein Trafficking.” PNAS, vol. 110, no. 19, National Academy of Sciences,
2013, pp. 7946–51, doi:10.1073/pnas.1220205110.
short: Y. Du, R. Tejos, M. Beck, E. Himschoot, H. Li, S. Robatzek, S. Vanneste,
J. Friml, PNAS 110 (2013) 7946–7951.
corr_author: '1'
date_created: 2018-12-11T11:59:48Z
date_published: 2013-05-07T00:00:00Z
date_updated: 2025-09-29T13:52:38Z
day: '07'
department:
- _id: JiFr
doi: 10.1073/pnas.1220205110
external_id:
isi:
- '000319327700089'
pmid:
- '23613581'
fulldoi: https://doi.org/10.1073/pnas.1220205110
intvolume: ' 110'
isi: 1
issue: '19'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651428/
month: '05'
oa: 1
oa_version: Submitted Version
page: 7946 - 7951
pmid: 1
project:
- _id: 2574781E-B435-11E9-9278-68D0E5697425
name: Koerber Prize
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3972'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Salicylic acid interferes with clathrin-mediated endocytic protein trafficking
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 110
year: '2013'
...
---
_id: '2832'
abstract:
- lang: eng
text: PIN-FORMED (PIN) proteins localize asymmetrically at the plasma membrane and
mediate intercellular polar transport of the plant hormone auxin that is crucial
for a multitude of developmental processes in plants. PIN localization is under
extensive control by environmental or developmental cues, but mechanisms regulating
PIN localization are not fully understood. Here we show that early endosomal components
ARF GEF BEN1 and newly identified Sec1/Munc18 family protein BEN2 are involved
in distinct steps of early endosomal trafficking. BEN1 and BEN2 are collectively
required for polar PIN localization, for their dynamic repolarization, and consequently
for auxin activity gradient formation and auxin-related developmental processes
including embryonic patterning, organogenesis, and vasculature venation patterning.
These results show that early endosomal trafficking is crucial for cell polarity
and auxin-dependent regulation of plant architecture.
article_number: e1003540
article_processing_charge: No
author:
- first_name: Hirokazu
full_name: Tanaka, Hirokazu
last_name: Tanaka
- first_name: Saeko
full_name: Kitakura, Saeko
last_name: Kitakura
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
- first_name: Tomohiro
full_name: Uemura, Tomohiro
last_name: Uemura
- first_name: Mugurel
full_name: Feraru, Mugurel
last_name: Feraru
- first_name: Riet
full_name: De Rycke, Riet
last_name: De Rycke
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Tatsuo
full_name: Kakimoto, Tatsuo
last_name: Kakimoto
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tanaka H, Kitakura S, Rakusová H, et al. Cell polarity and patterning by PIN
trafficking through early endosomal compartments in arabidopsis thaliana. PLoS
Genetics. 2013;9(5). doi:10.1371/journal.pgen.1003540
apa: Tanaka, H., Kitakura, S., Rakusová, H., Uemura, T., Feraru, M., De Rycke, R.,
… Friml, J. (2013). Cell polarity and patterning by PIN trafficking through early
endosomal compartments in arabidopsis thaliana. PLoS Genetics. Public Library
of Science. https://doi.org/10.1371/journal.pgen.1003540
chicago: Tanaka, Hirokazu, Saeko Kitakura, Hana Rakusová, Tomohiro Uemura, Mugurel
Feraru, Riet De Rycke, Stéphanie Robert, Tatsuo Kakimoto, and Jiří Friml. “Cell
Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments
in Arabidopsis Thaliana.” PLoS Genetics. Public Library of Science, 2013.
https://doi.org/10.1371/journal.pgen.1003540.
ieee: H. Tanaka et al., “Cell polarity and patterning by PIN trafficking
through early endosomal compartments in arabidopsis thaliana,” PLoS Genetics,
vol. 9, no. 5. Public Library of Science, 2013.
ista: Tanaka H, Kitakura S, Rakusová H, Uemura T, Feraru M, De Rycke R, Robert S,
Kakimoto T, Friml J. 2013. Cell polarity and patterning by PIN trafficking through
early endosomal compartments in arabidopsis thaliana. PLoS Genetics. 9(5), e1003540.
mla: Tanaka, Hirokazu, et al. “Cell Polarity and Patterning by PIN Trafficking through
Early Endosomal Compartments in Arabidopsis Thaliana.” PLoS Genetics, vol.
9, no. 5, e1003540, Public Library of Science, 2013, doi:10.1371/journal.pgen.1003540.
short: H. Tanaka, S. Kitakura, H. Rakusová, T. Uemura, M. Feraru, R. De Rycke, S.
Robert, T. Kakimoto, J. Friml, PLoS Genetics 9 (2013).
corr_author: '1'
date_created: 2018-12-11T11:59:50Z
date_published: 2013-05-05T00:00:00Z
date_updated: 2025-09-29T13:50:00Z
day: '05'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1371/journal.pgen.1003540
ec_funded: 1
external_id:
isi:
- '000320030000043'
file:
- access_level: open_access
checksum: 050237d6c53e8d1601b26808ee1dd6d8
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:39Z
date_updated: 2020-07-14T12:45:50Z
file_id: '4957'
file_name: IST-2016-411-v1+1_journal.pgen.1003540.pdf
file_size: 3813091
relation: main_file
file_date_updated: 2020-07-14T12:45:50Z
fulldoi: https://doi.org/10.1371/journal.pgen.1003540
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
issue: '5'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PLoS Genetics
publication_status: published
publisher: Public Library of Science
publist_id: '3967'
pubrep_id: '411'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell polarity and patterning by PIN trafficking through early endosomal compartments
in arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 9
year: '2013'
...
---
_id: '2835'
abstract:
- lang: eng
text: The phytohormone auxin regulates virtually every aspect of plant development.
To identify new genes involved in auxin activity, a genetic screen was performed
for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the
auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen,
designated as weak auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression
when treated with the synthetic auxin 2,4-dichlorophenoxyacetic acid and displays
severe defects in root development. The wxr3 mutant decreases polar auxin transport
and results in a disruption of the asymmetric auxin distribution. The levels of
the auxin transporters AUXIN1 and PIN-FORMED are dramatically reduced in the wxr3
root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1
(RUS1), a member of the conserved Domain of Unknown Function647 protein family
found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an
essential role in the regulation of polar auxin transport by maintaining the proper
level of auxin transporters on the plasma membrane.
article_processing_charge: No
author:
- first_name: Hong
full_name: Yu, Hong
last_name: Yu
- first_name: Michael
full_name: Karampelias, Michael
last_name: Karampelias
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Wendy
full_name: Peer, Wendy
last_name: Peer
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Songqing
full_name: Ye, Songqing
last_name: Ye
- first_name: Lei
full_name: Ge, Lei
last_name: Ge
- first_name: Jerry
full_name: Cohen, Jerry
last_name: Cohen
- first_name: Angus
full_name: Murphy, Angus
last_name: Murphy
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Mark
full_name: Estelle, Mark
last_name: Estelle
citation:
ama: Yu H, Karampelias M, Robert S, et al. Root ultraviolet b-sensitive1/weak auxin
response3 is essential for polar auxin transport in arabidopsis. Plant Physiology.
2013;162(2):965-976. doi:10.1104/pp.113.217018
apa: Yu, H., Karampelias, M., Robert, S., Peer, W., Swarup, R., Ye, S., … Estelle,
M. (2013). Root ultraviolet b-sensitive1/weak auxin response3 is essential for
polar auxin transport in arabidopsis. Plant Physiology. American Society
of Plant Biologists. https://doi.org/10.1104/pp.113.217018
chicago: Yu, Hong, Michael Karampelias, Stéphanie Robert, Wendy Peer, Ranjan Swarup,
Songqing Ye, Lei Ge, et al. “Root Ultraviolet B-Sensitive1/Weak Auxin Response3
Is Essential for Polar Auxin Transport in Arabidopsis.” Plant Physiology.
American Society of Plant Biologists, 2013. https://doi.org/10.1104/pp.113.217018.
ieee: H. Yu et al., “Root ultraviolet b-sensitive1/weak auxin response3 is
essential for polar auxin transport in arabidopsis,” Plant Physiology,
vol. 162, no. 2. American Society of Plant Biologists, pp. 965–976, 2013.
ista: Yu H, Karampelias M, Robert S, Peer W, Swarup R, Ye S, Ge L, Cohen J, Murphy
A, Friml J, Estelle M. 2013. Root ultraviolet b-sensitive1/weak auxin response3
is essential for polar auxin transport in arabidopsis. Plant Physiology. 162(2),
965–976.
mla: Yu, Hong, et al. “Root Ultraviolet B-Sensitive1/Weak Auxin Response3 Is Essential
for Polar Auxin Transport in Arabidopsis.” Plant Physiology, vol. 162,
no. 2, American Society of Plant Biologists, 2013, pp. 965–76, doi:10.1104/pp.113.217018.
short: H. Yu, M. Karampelias, S. Robert, W. Peer, R. Swarup, S. Ye, L. Ge, J. Cohen,
A. Murphy, J. Friml, M. Estelle, Plant Physiology 162 (2013) 965–976.
date_created: 2018-12-11T11:59:51Z
date_published: 2013-06-01T00:00:00Z
date_updated: 2025-09-29T13:48:15Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.113.217018
external_id:
isi:
- '000319819900034'
pmid:
- '23580592'
fulldoi: https://doi.org/10.1104/pp.113.217018
intvolume: ' 162'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668084/
month: '06'
oa: 1
oa_version: Submitted Version
page: 965 - 976
pmid: 1
publication: Plant Physiology
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3964'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin
transport in arabidopsis
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 162
year: '2013'
...
---
_id: '2844'
abstract:
- lang: eng
text: As soon as a seed germinates, plant growth relates to gravity to ensure that
the root penetrates the soil and the shoot expands aerially. Whereas mechanisms
of positive and negative orthogravitropism of primary roots and shoots are relatively
well understood [1-3], lateral organs often show more complex growth behavior
[4]. Lateral roots (LRs) seemingly suppress positive gravitropic growth and show
a defined gravitropic set-point angle (GSA) that allows radial expansion of the
root system (plagiotropism) [3, 4]. Despite its eminent importance for root architecture,
it so far remains completely unknown how lateral organs partially suppress positive
orthogravitropism. Here we show that the phytohormone auxin steers GSA formation
and limits positive orthogravitropism in LR. Low and high auxin levels/signaling
lead to radial or axial root systems, respectively. At a cellular level, it is
the auxin transport-dependent regulation of asymmetric growth in the elongation
zone that determines GSA. Our data suggest that strong repression of PIN4/PIN7
and transient PIN3 expression limit auxin redistribution in young LR columella
cells. We conclude that PIN activity, by temporally limiting the asymmetric auxin
fluxes in the tip of LRs, induces transient, differential growth responses in
the elongation zone and, consequently, controls root architecture.
article_processing_charge: No
author:
- first_name: Michel
full_name: Rosquete, Michel
last_name: Rosquete
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Elke
full_name: Barbez, Elke
last_name: Barbez
- first_name: Ernst
full_name: Stelzer, Ernst
last_name: Stelzer
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Alexis
full_name: Maizel, Alexis
last_name: Maizel
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
citation:
ama: Rosquete M, von Wangenheim D, Marhavý P, et al. An auxin transport mechanism
restricts positive orthogravitropism in lateral roots. Current Biology.
2013;23(9):817-822. doi:10.1016/j.cub.2013.03.064
apa: Rosquete, M., von Wangenheim, D., Marhavý, P., Barbez, E., Stelzer, E., Benková,
E., … Kleine Vehn, J. (2013). An auxin transport mechanism restricts positive
orthogravitropism in lateral roots. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2013.03.064
chicago: Rosquete, Michel, Daniel von Wangenheim, Peter Marhavý, Elke Barbez, Ernst
Stelzer, Eva Benková, Alexis Maizel, and Jürgen Kleine Vehn. “An Auxin Transport
Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” Current Biology.
Cell Press, 2013. https://doi.org/10.1016/j.cub.2013.03.064.
ieee: M. Rosquete et al., “An auxin transport mechanism restricts positive
orthogravitropism in lateral roots,” Current Biology, vol. 23, no. 9. Cell
Press, pp. 817–822, 2013.
ista: Rosquete M, von Wangenheim D, Marhavý P, Barbez E, Stelzer E, Benková E, Maizel
A, Kleine Vehn J. 2013. An auxin transport mechanism restricts positive orthogravitropism
in lateral roots. Current Biology. 23(9), 817–822.
mla: Rosquete, Michel, et al. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism
in Lateral Roots.” Current Biology, vol. 23, no. 9, Cell Press, 2013, pp.
817–22, doi:10.1016/j.cub.2013.03.064.
short: M. Rosquete, D. von Wangenheim, P. Marhavý, E. Barbez, E. Stelzer, E. Benková,
A. Maizel, J. Kleine Vehn, Current Biology 23 (2013) 817–822.
date_created: 2018-12-11T11:59:53Z
date_published: 2013-05-06T00:00:00Z
date_updated: 2025-09-29T13:43:30Z
day: '06'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cub.2013.03.064
ec_funded: 1
external_id:
isi:
- '000318750900035'
fulldoi: https://doi.org/10.1016/j.cub.2013.03.064
intvolume: ' 23'
isi: 1
issue: '9'
language:
- iso: eng
month: '05'
oa_version: None
page: 817 - 822
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '3950'
quality_controlled: '1'
scopus_import: '1'
status: public
title: An auxin transport mechanism restricts positive orthogravitropism in lateral
roots
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 23
year: '2013'
...
---
_id: '2882'
abstract:
- lang: eng
text: Gravitropic bending of plant organs is mediated by an asymmetric signaling
of the plant hormone auxin between the upper and lower side of the respective
organ. Here, we show that also another plant hormone, gibberellic acid (GA), shows
asymmetric action during gravitropic responses. Immunodetection using an antibody
against GA and monitoring GA signaling output by downstream degradation of DELLA
proteins revealed an asymmetric GA distribution and response with the maximum
at the lower side of gravistimulated roots. Genetic or pharmacological manipulation
of GA levels or response affects gravity-mediated auxin redistribution and root
bending response. The higher GA levels at the lower side of the root correlate
with increased amounts of PIN-FORMED2 (PIN2) auxin transporter at the plasma membrane.
The observed increase in PIN2 stability is caused by a specific GA effect on trafficking
of PIN proteins to lytic vacuoles that presumably occurs downstream of brefeldin
A-sensitive endosomes. Our results suggest that asymmetric auxin distribution
instructive for gravity-induced differential growth is consolidated by the asymmetric
action of GA that stabilizes the PIN-dependent auxin stream along the lower side
of gravistimulated roots.
article_processing_charge: No
author:
- first_name: Christian
full_name: Löfke, Christian
last_name: Löfke
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Ingo
full_name: Heilmann, Ingo
last_name: Heilmann
- first_name: Marc
full_name: Van Montagu, Marc
last_name: Van Montagu
- first_name: Thomas
full_name: Teichmann, Thomas
last_name: Teichmann
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Löfke C, Zwiewka M, Heilmann I, Van Montagu M, Teichmann T, Friml J. Asymmetric
gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters
during root gravitropism. PNAS. 2013;110(9):3627-3632. doi:10.1073/pnas.1300107110
apa: Löfke, C., Zwiewka, M., Heilmann, I., Van Montagu, M., Teichmann, T., &
Friml, J. (2013). Asymmetric gibberellin signaling regulates vacuolar trafficking
of PIN auxin transporters during root gravitropism. PNAS. National Academy
of Sciences. https://doi.org/10.1073/pnas.1300107110
chicago: Löfke, Christian, Marta Zwiewka, Ingo Heilmann, Marc Van Montagu, Thomas
Teichmann, and Jiří Friml. “Asymmetric Gibberellin Signaling Regulates Vacuolar
Trafficking of PIN Auxin Transporters during Root Gravitropism.” PNAS.
National Academy of Sciences, 2013. https://doi.org/10.1073/pnas.1300107110.
ieee: C. Löfke, M. Zwiewka, I. Heilmann, M. Van Montagu, T. Teichmann, and J. Friml,
“Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin
transporters during root gravitropism,” PNAS, vol. 110, no. 9. National
Academy of Sciences, pp. 3627–3632, 2013.
ista: Löfke C, Zwiewka M, Heilmann I, Van Montagu M, Teichmann T, Friml J. 2013.
Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters
during root gravitropism. PNAS. 110(9), 3627–3632.
mla: Löfke, Christian, et al. “Asymmetric Gibberellin Signaling Regulates Vacuolar
Trafficking of PIN Auxin Transporters during Root Gravitropism.” PNAS,
vol. 110, no. 9, National Academy of Sciences, 2013, pp. 3627–32, doi:10.1073/pnas.1300107110.
short: C. Löfke, M. Zwiewka, I. Heilmann, M. Van Montagu, T. Teichmann, J. Friml,
PNAS 110 (2013) 3627–3632.
date_created: 2018-12-11T12:00:07Z
date_published: 2013-02-26T00:00:00Z
date_updated: 2025-09-29T13:34:38Z
day: '26'
department:
- _id: JiFr
doi: 10.1073/pnas.1300107110
external_id:
isi:
- '000315841900083'
pmid:
- '23391733'
fulldoi: https://doi.org/10.1073/pnas.1300107110
intvolume: ' 110'
isi: 1
issue: '9'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587205/
month: '02'
oa: 1
oa_version: Submitted Version
page: 3627 - 3632
pmid: 1
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3879'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin
transporters during root gravitropism
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 110
year: '2013'
...
---
_id: '2883'
abstract:
- lang: eng
text: Plant architecture is influenced by the polar, cell-to-cell transport of auxin
that is primarily provided and regulated by plasma membrane efflux catalysts of
the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were
shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1
(TWD1), although underlying mechanisms are unclear. By genetic manipulation of
TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and,
thus, downstream developmental traits, such as epidermal twisting and gravitropism
of the root. Using immunological assays, we demonstrate a predominant lateral,
mainly outward-facing, plasma membrane location for TWD1 in the root epidermis
characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3.
At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1.
In planta bioluminescence resonance energy transfer analysis was used to verify
specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model
in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein
interaction at the plasma membrane, minimizing reflux from the root apoplast into
the cytoplasm.
acknowledgement: We would thank Vincent Vincenzetti and Laurence Charrier for excellent
technical assistance, A. von Arnim for the donation of BRET vectors, E. Spalding
for TWD1-CFP, TWD1-CFP/29-1-GFP/ER-YFP, and ABCB4-GFP lines, M. Palmgren for discussion
and support, and E. Martinoia for TT12 cDNA, support, and mentorship. Imaging data
were partially collected at the Center for Advanced Bioimaging, University of Copenhagen,
Denmark. This work was supported by grants from the Novartis Foundation (to M.G.),
from the Danish Research School for Biotechnology (to M.G. and A.S.), from the Forschungskredit
of the University of Zurich (to A.B.), from the Pool de Recherche of the University
of Fribourg (to M.G.), and from the Swiss National Funds (to M.G.). M.G. dedicates
this work to his father, who passed away during the resubmission process.
article_processing_charge: No
author:
- first_name: Bangjun
full_name: Wang, Bangjun
last_name: Wang
- first_name: Aurélien
full_name: Bailly, Aurélien
last_name: Bailly
- first_name: Marta
full_name: Zwiewk, Marta
last_name: Zwiewk
- first_name: Sina
full_name: Henrichs, Sina
last_name: Henrichs
- first_name: Elisa
full_name: Azzarello, Elisa
last_name: Azzarello
- first_name: Stefano
full_name: Mancuso, Stefano
last_name: Mancuso
- first_name: Masayoshi
full_name: Maeshima, Masayoshi
last_name: Maeshima
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Alexander
full_name: Schulz, Alexander
last_name: Schulz
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
citation:
ama: Wang B, Bailly A, Zwiewk M, et al. Arabidopsis TWISTED DWARF1 functionally
interacts with auxin exporter ABCB1 on the root plasma membrane. Plant Cell.
2013;25(1):202-214. doi:10.1105/tpc.112.105999
apa: Wang, B., Bailly, A., Zwiewk, M., Henrichs, S., Azzarello, E., Mancuso, S.,
… Geisler, M. (2013). Arabidopsis TWISTED DWARF1 functionally interacts with auxin
exporter ABCB1 on the root plasma membrane. Plant Cell. American Society
of Plant Biologists. https://doi.org/10.1105/tpc.112.105999
chicago: Wang, Bangjun, Aurélien Bailly, Marta Zwiewk, Sina Henrichs, Elisa Azzarello,
Stefano Mancuso, Masayoshi Maeshima, Jiří Friml, Alexander Schulz, and Markus
Geisler. “Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter
ABCB1 on the Root Plasma Membrane.” Plant Cell. American Society of Plant
Biologists, 2013. https://doi.org/10.1105/tpc.112.105999.
ieee: B. Wang et al., “Arabidopsis TWISTED DWARF1 functionally interacts
with auxin exporter ABCB1 on the root plasma membrane,” Plant Cell, vol.
25, no. 1. American Society of Plant Biologists, pp. 202–214, 2013.
ista: Wang B, Bailly A, Zwiewk M, Henrichs S, Azzarello E, Mancuso S, Maeshima M,
Friml J, Schulz A, Geisler M. 2013. Arabidopsis TWISTED DWARF1 functionally interacts
with auxin exporter ABCB1 on the root plasma membrane. Plant Cell. 25(1), 202–214.
mla: Wang, Bangjun, et al. “Arabidopsis TWISTED DWARF1 Functionally Interacts with
Auxin Exporter ABCB1 on the Root Plasma Membrane.” Plant Cell, vol. 25,
no. 1, American Society of Plant Biologists, 2013, pp. 202–14, doi:10.1105/tpc.112.105999.
short: B. Wang, A. Bailly, M. Zwiewk, S. Henrichs, E. Azzarello, S. Mancuso, M.
Maeshima, J. Friml, A. Schulz, M. Geisler, Plant Cell 25 (2013) 202–214.
date_created: 2018-12-11T12:00:08Z
date_published: 2013-01-01T00:00:00Z
date_updated: 2025-09-29T13:34:07Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.112.105999
external_id:
isi:
- '000315572400017'
pmid:
- '23321285'
fulldoi: https://doi.org/10.1105/tpc.112.105999
intvolume: ' 25'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584535/
month: '01'
oa: 1
oa_version: Submitted Version
page: 202 - 214
pmid: 1
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3878'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1
on the root plasma membrane
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 25
year: '2013'
...
---
_id: '2919'
abstract:
- lang: eng
text: The distribution of the phytohormone auxin regulates many aspects of plant
development including growth response to gravity. Gravitropic root curvature involves
coordinated and asymmetric cell elongation between the lower and upper side of
the root, mediated by differential cellular auxin levels. The asymmetry in the
auxin distribution is established and maintained by a spatio-temporal regulation
of the PIN-FORMED (PIN) auxin transporter activity. We provide novel insights
into the complex regulation of PIN abundance and activity during root gravitropism.
We show that PIN2 turnover is differentially regulated on the upper and lower
side of gravistimulated roots by distinct but partially overlapping auxin feedback
mechanisms. In addition to regulating transcription and clathrin-mediated internalization,
auxin also controls PIN abundance at the plasma membrane by promoting their vacuolar
targeting and degradation. This effect of elevated auxin levels requires the activity
of SKP-Cullin-F-box TIR1/AFB (SCF TIR1/AFB)-dependent pathway. Importantly, also
suboptimal auxin levels mediate PIN degradation utilizing the same signalling
pathway. These feedback mechanisms are functionally important during gravitropic
response and ensure fine-tuning of auxin fluxes for maintaining as well as terminating
asymmetric growth.
article_processing_charge: No
author:
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Bert
full_name: De Rybel, Bert
last_name: De Rybel
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Baster P, Robert S, Kleine Vehn J, et al. SCF^TIR1 AFB-auxin signalling regulates
PIN vacuolar trafficking and auxin fluxes during root gravitropism. EMBO Journal.
2013;32(2):260-274. doi:10.1038/emboj.2012.310
apa: Baster, P., Robert, S., Kleine Vehn, J., Vanneste, S., Kania, U., Grunewald,
W., … Friml, J. (2013). SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking
and auxin fluxes during root gravitropism. EMBO Journal. Wiley-Blackwell.
https://doi.org/10.1038/emboj.2012.310
chicago: Baster, Pawel, Stéphanie Robert, Jürgen Kleine Vehn, Steffen Vanneste,
Urszula Kania, Wim Grunewald, Bert De Rybel, Tom Beeckman, and Jiří Friml. “SCF^TIR1
AFB-Auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes during
Root Gravitropism.” EMBO Journal. Wiley-Blackwell, 2013. https://doi.org/10.1038/emboj.2012.310.
ieee: P. Baster et al., “SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar
trafficking and auxin fluxes during root gravitropism,” EMBO Journal, vol.
32, no. 2. Wiley-Blackwell, pp. 260–274, 2013.
ista: Baster P, Robert S, Kleine Vehn J, Vanneste S, Kania U, Grunewald W, De Rybel
B, Beeckman T, Friml J. 2013. SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar
trafficking and auxin fluxes during root gravitropism. EMBO Journal. 32(2), 260–274.
mla: Baster, Pawel, et al. “SCF^TIR1 AFB-Auxin Signalling Regulates PIN Vacuolar
Trafficking and Auxin Fluxes during Root Gravitropism.” EMBO Journal, vol.
32, no. 2, Wiley-Blackwell, 2013, pp. 260–74, doi:10.1038/emboj.2012.310.
short: P. Baster, S. Robert, J. Kleine Vehn, S. Vanneste, U. Kania, W. Grunewald,
B. De Rybel, T. Beeckman, J. Friml, EMBO Journal 32 (2013) 260–274.
corr_author: '1'
date_created: 2018-12-11T12:00:20Z
date_published: 2013-01-23T00:00:00Z
date_updated: 2025-09-29T13:28:19Z
day: '23'
department:
- _id: JiFr
doi: 10.1038/emboj.2012.310
external_id:
isi:
- '000316464500009'
pmid:
- '23211744'
fulldoi: https://doi.org/10.1038/emboj.2012.310
intvolume: ' 32'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553380/
month: '01'
oa: 1
oa_version: Submitted Version
page: 260 - 274
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3818'
quality_controlled: '1'
scopus_import: '1'
status: public
title: SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin
fluxes during root gravitropism
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 32
year: '2013'
...
---
_id: '507'
abstract:
- lang: eng
text: Fertilization in flowering plants requires the temporal and spatial coordination
of many developmental processes, including pollen production, anther dehiscence,
ovule production, and pollen tube elongation. However, it remains elusive as to
how this coordination occurs during reproduction. Here, we present evidence that
endocytosis, involving heterotetrameric adaptor protein complex 2 (AP-2), plays
a crucial role in fertilization. An Arabidopsis thaliana mutant ap2m displays
multiple defects in pollen production and viability, as well as elongation of
staminal filaments and pollen tubes, all of which are pivotal processes needed
for fertilization. Of these abnormalities, the defects in elongation of staminal
filaments and pollen tubes were partially rescued by exogenous auxin. Moreover,
DR5rev:GFP (for green fluorescent protein) expression was greatly reduced in filaments
and anthers in ap2m mutant plants. At the cellular level, ap2m mutants displayed
defects in both endocytosis of N-(3-triethylammonium-propyl)-4- (4-diethylaminophenylhexatrienyl)
pyridinium dibromide, a lypophilic dye used as an endocytosis marker, and polar
localization of auxin-efflux carrier PIN FORMED2 (PIN2) in the stamen filaments.
Moreover, these defects were phenocopied by treatment with Tyrphostin A23, an
inhibitor of endocytosis. Based on these results, we propose that AP-2-dependent
endocytosis plays a crucial role in coordinating the multiple developmental aspects
of male reproductive organs by modulating cellular auxin level through the regulation
of the amount and polarity of PINs.
article_processing_charge: No
author:
- first_name: Soo
full_name: Kim, Soo
last_name: Kim
- first_name: Zheng
full_name: Xu, Zheng
last_name: Xu
- first_name: Kyungyoung
full_name: Song, Kyungyoung
last_name: Song
- first_name: Dae
full_name: Kim, Dae
last_name: Kim
- first_name: Hyangju
full_name: Kang, Hyangju
last_name: Kang
- first_name: Ilka
full_name: Reichardt, Ilka
last_name: Reichardt
- first_name: Eun
full_name: Sohn, Eun
last_name: Sohn
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Gerd
full_name: Juergens, Gerd
last_name: Juergens
- first_name: Inhwan
full_name: Hwang, Inhwan
last_name: Hwang
citation:
ama: Kim S, Xu Z, Song K, et al. Adaptor protein complex 2-mediated endocytosis
is crucial for male reproductive organ development in arabidopsis. Plant Cell.
2013;25(8):2970-2985. doi:10.1105/tpc.113.114264
apa: Kim, S., Xu, Z., Song, K., Kim, D., Kang, H., Reichardt, I., … Hwang, I. (2013).
Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive
organ development in arabidopsis. Plant Cell. American Society of Plant
Biologists. https://doi.org/10.1105/tpc.113.114264
chicago: Kim, Soo, Zheng Xu, Kyungyoung Song, Dae Kim, Hyangju Kang, Ilka Reichardt,
Eun Sohn, Jiří Friml, Gerd Juergens, and Inhwan Hwang. “Adaptor Protein Complex
2-Mediated Endocytosis Is Crucial for Male Reproductive Organ Development in Arabidopsis.”
Plant Cell. American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.113.114264.
ieee: S. Kim et al., “Adaptor protein complex 2-mediated endocytosis is crucial
for male reproductive organ development in arabidopsis,” Plant Cell, vol.
25, no. 8. American Society of Plant Biologists, pp. 2970–2985, 2013.
ista: Kim S, Xu Z, Song K, Kim D, Kang H, Reichardt I, Sohn E, Friml J, Juergens
G, Hwang I. 2013. Adaptor protein complex 2-mediated endocytosis is crucial for
male reproductive organ development in arabidopsis. Plant Cell. 25(8), 2970–2985.
mla: Kim, Soo, et al. “Adaptor Protein Complex 2-Mediated Endocytosis Is Crucial
for Male Reproductive Organ Development in Arabidopsis.” Plant Cell, vol.
25, no. 8, American Society of Plant Biologists, 2013, pp. 2970–85, doi:10.1105/tpc.113.114264.
short: S. Kim, Z. Xu, K. Song, D. Kim, H. Kang, I. Reichardt, E. Sohn, J. Friml,
G. Juergens, I. Hwang, Plant Cell 25 (2013) 2970–2985.
date_created: 2018-12-11T11:46:52Z
date_published: 2013-08-01T00:00:00Z
date_updated: 2025-09-30T07:17:15Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.113.114264
external_id:
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- '000324920500019'
pmid:
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fulldoi: https://doi.org/10.1105/tpc.113.114264
intvolume: ' 25'
isi: 1
issue: '8'
language:
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main_file_link:
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month: '08'
oa: 1
oa_version: Submitted Version
page: 2970 - 2985
pmid: 1
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7312'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive
organ development in arabidopsis
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 25
year: '2013'
...