---
_id: '10282'
abstract:
- lang: eng
text: Advanced transcriptome sequencing has revealed that the majority of eukaryotic
genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated
to investigating the functional relevance of particular splicing events, even
those in the key developmental and hormonal regulators. Combining approaches of
genetics, biochemistry and advanced confocal microscopy, we describe the impact
of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana.
PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces
two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing
in a four amino acid stretch, exhibit almost identical expression patterns and
subcellular localization. We reveal that they are closely associated and mutually
influence each other's mobility within the plasma membrane. Phenotypic complementation
tests indicate that the functional contribution of PIN7b per se is minor, but
it markedly reduces the prominent PIN7a activity, which is required for correct
seedling apical hook formation and auxin-mediated tropic responses. Our results
establish alternative splicing of the PIN family as a conserved, functionally
relevant mechanism, revealing an additional regulatory level of auxin-mediated
plant development.
acknowledgement: We thank Claus Schwechheimer for the pin34 and pin347 seeds, Yuliia
Mironova for technical assistance, Ksenia Timofeyenko and Dmitry Konovalov for help
with the evolutional analysis, Konstantin Kutashev and Siarhei Dabravolski for assistance
with FRET-FLIM, Huibin Han for advice with hypocotyl imaging, Karel Müller for the
initial qRT-PCR on the tobacco cell lines, Stano Pekár for suggestions regarding
the statistical analysis of the morphodynamic measurements, and Jozef Mravec, Dolf
Weijers and Lindy Abas for their comments on the manuscript. This work was supported
by the Czech Science Foundation (projects 16-26428S and 19-23773S to IK, MH and
KRůžička, 19-18917S to JHumpolíčková and 18-26981S to JF), and the Ministry of Education,
Youth and Sports of the Czech Republic (MEYS, CZ.02.1.01/0.0/0.0/16_019/0000738)
to KRůžička and JHejátko. The imaging facilities of the Institute of Experimental
Botany and CEITEC are supported by MEYS (LM2018129 – Czech BioImaging and CZ.02.1.01/0.0/0.0/16_013/0001775).
The authors declare no competing interests.
article_processing_charge: No
article_type: original
author:
- first_name: Ivan
full_name: Kashkan, Ivan
last_name: Kashkan
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Katarzyna
full_name: Retzer, Katarzyna
last_name: Retzer
- first_name: Jana
full_name: Humpolíčková, Jana
last_name: Humpolíčková
- first_name: Aswathy
full_name: Jayasree, Aswathy
last_name: Jayasree
- first_name: Roberta
full_name: Filepová, Roberta
last_name: Filepová
- first_name: Zuzana
full_name: Vondráková, Zuzana
last_name: Vondráková
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Debbie
full_name: Rombaut, Debbie
last_name: Rombaut
- first_name: Thomas B.
full_name: Jacobs, Thomas B.
last_name: Jacobs
- first_name: Mikko J.
full_name: Frilander, Mikko J.
last_name: Frilander
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Kamil
full_name: Růžička, Kamil
last_name: Růžička
citation:
ama: Kashkan I, Hrtyan M, Retzer K, et al. Mutually opposing activity of PIN7 splicing
isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana.
New Phytologist. 2022;233(1):329-343. doi:10.1111/nph.17792
apa: Kashkan, I., Hrtyan, M., Retzer, K., Humpolíčková, J., Jayasree, A., Filepová,
R., … Růžička, K. (2022). Mutually opposing activity of PIN7 splicing isoforms
is required for auxin-mediated tropic responses in Arabidopsis thaliana. New
Phytologist. Wiley. https://doi.org/10.1111/nph.17792
chicago: Kashkan, Ivan, Mónika Hrtyan, Katarzyna Retzer, Jana Humpolíčková, Aswathy
Jayasree, Roberta Filepová, Zuzana Vondráková, et al. “Mutually Opposing Activity
of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis
Thaliana.” New Phytologist. Wiley, 2022. https://doi.org/10.1111/nph.17792.
ieee: I. Kashkan et al., “Mutually opposing activity of PIN7 splicing isoforms
is required for auxin-mediated tropic responses in Arabidopsis thaliana,” New
Phytologist, vol. 233, no. 1. Wiley, pp. 329–343, 2022.
ista: Kashkan I, Hrtyan M, Retzer K, Humpolíčková J, Jayasree A, Filepová R, Vondráková
Z, Simon S, Rombaut D, Jacobs TB, Frilander MJ, Hejátko J, Friml J, Petrášek J,
Růžička K. 2022. Mutually opposing activity of PIN7 splicing isoforms is required
for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist.
233(1), 329–343.
mla: Kashkan, Ivan, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms
Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” New
Phytologist, vol. 233, no. 1, Wiley, 2022, pp. 329–43, doi:10.1111/nph.17792.
short: I. Kashkan, M. Hrtyan, K. Retzer, J. Humpolíčková, A. Jayasree, R. Filepová,
Z. Vondráková, S. Simon, D. Rombaut, T.B. Jacobs, M.J. Frilander, J. Hejátko,
J. Friml, J. Petrášek, K. Růžička, New Phytologist 233 (2022) 329–343.
date_created: 2021-11-14T23:01:24Z
date_published: 2022-01-01T00:00:00Z
date_updated: 2024-05-22T11:33:15Z
day: '01'
department:
- _id: JiFr
doi: 10.1111/nph.17792
external_id:
isi:
- '000714678100001'
pmid:
- '34637542'
intvolume: ' 233'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.biorxiv.org/content/10.1101/2020.05.02.074070v2
month: '01'
oa: 1
oa_version: Preprint
page: 329-343
pmid: 1
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated
tropic responses in Arabidopsis thaliana
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 233
year: '2022'
...
---
_id: '10411'
abstract:
- lang: eng
text: The phytohormone auxin is the major growth regulator governing tropic responses
including gravitropism. Auxin build-up at the lower side of stimulated shoots
promotes cell expansion, whereas in roots it inhibits growth, leading to upward
shoot bending and downward root bending, respectively. Yet it remains an enigma
how the same signal can trigger such opposite cellular responses. In this review,
we discuss several recent unexpected insights into the mechanisms underlying auxin
regulation of growth, challenging several existing models. We focus on the divergent
mechanisms of apoplastic pH regulation in shoots and roots revisiting the classical
Acid Growth Theory and discuss coordinated involvement of multiple auxin signaling
pathways. From this emerges a more comprehensive, updated picture how auxin regulates
growth.
acknowledgement: The authors thank Alexandra Mally for editing the text. This work
was supported by the Austrian Science Fund (FWF) I 3630-B25 to Jiří Friml and the
DOC Fellowship of the Austrian Academy of Sciences to Lanxin Li. All figures were
created with BioRender.com.
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Li L, Gallei MC, Friml J. Bending to auxin: Fast acid growth for tropisms.
Trends in Plant Science. 2022;27(5):440-449. doi:10.1016/j.tplants.2021.11.006'
apa: 'Li, L., Gallei, M. C., & Friml, J. (2022). Bending to auxin: Fast acid
growth for tropisms. Trends in Plant Science. Cell Press. https://doi.org/10.1016/j.tplants.2021.11.006'
chicago: 'Li, Lanxin, Michelle C Gallei, and Jiří Friml. “Bending to Auxin: Fast
Acid Growth for Tropisms.” Trends in Plant Science. Cell Press, 2022. https://doi.org/10.1016/j.tplants.2021.11.006.'
ieee: 'L. Li, M. C. Gallei, and J. Friml, “Bending to auxin: Fast acid growth for
tropisms,” Trends in Plant Science, vol. 27, no. 5. Cell Press, pp. 440–449,
2022.'
ista: 'Li L, Gallei MC, Friml J. 2022. Bending to auxin: Fast acid growth for tropisms.
Trends in Plant Science. 27(5), 440–449.'
mla: 'Li, Lanxin, et al. “Bending to Auxin: Fast Acid Growth for Tropisms.” Trends
in Plant Science, vol. 27, no. 5, Cell Press, 2022, pp. 440–49, doi:10.1016/j.tplants.2021.11.006.'
short: L. Li, M.C. Gallei, J. Friml, Trends in Plant Science 27 (2022) 440–449.
corr_author: '1'
date_created: 2021-12-05T23:01:43Z
date_published: 2022-05-01T00:00:00Z
date_updated: 2026-04-07T14:18:57Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.tplants.2021.11.006
external_id:
isi:
- '000793707900005'
pmid:
- '34848141'
file:
- access_level: open_access
checksum: 3d94980ee1ff6bec100dd813f6a921a6
content_type: application/pdf
creator: amally
date_created: 2023-11-02T17:00:03Z
date_updated: 2023-11-02T17:00:03Z
file_id: '14480'
file_name: Li Plants 2021_accepted.pdf
file_size: 805779
relation: main_file
success: 1
file_date_updated: 2023-11-02T17:00:03Z
has_accepted_license: '1'
intvolume: ' 27'
isi: 1
issue: '5'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Submitted Version
page: 440-449
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Trends in Plant Science
publication_identifier:
issn:
- 1360-1385
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 'Bending to auxin: Fast acid growth for tropisms'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 27
year: '2022'
...
---
_id: '10583'
abstract:
- lang: eng
text: The synthetic strigolactone (SL) analog, rac-GR24, has been instrumental in
studying the role of SLs as well as karrikins because it activates the receptors
DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively.
Treatment with rac-GR24 modifies the root architecture at different levels, such
as decreasing the lateral root density (LRD), while promoting root hair elongation
or flavonol accumulation. Previously, we have shown that the flavonol biosynthesis
is transcriptionally activated in the root by rac-GR24 treatment, but, thus far,
the molecular players involved in that response have remained unknown. To get
an in-depth insight into the changes that occur after the compound is perceived
by the roots, we compared the root transcriptomes of the wild type and the more
axillary growth2 (max2) mutant, affected in both SL and karrikin signaling pathways,
with and without rac-GR24 treatment. Quantitative reverse transcription (qRT)-PCR,
reporter line analysis and mutant phenotyping indicated that the flavonol response
and the root hair elongation are controlled by the ELONGATED HYPOCOTYL 5 (HY5)
and MYB12 transcription factors, but HY5, in contrast to MYB12, affects the LRD
as well. Furthermore, we identified the transcription factors TARGET OF MONOPTEROS
5 (TMO5) and TMO5 LIKE1 as negative and the Mediator complex as positive regulators
of the rac-GR24 effect on LRD. Altogether, hereby, we get closer toward understanding
the molecular mechanisms that underlay the rac-GR24 responses in the root.
acknowledgement: The authors thank Ralf Stracke (Bielefeld University, Bielefeld,
Germany) for providing the myb mutants and their colleagues Bert De Rybel for the
tmo5t;mo5l1 double mutant, Boris Parizot for tips on the RNA-seq analysis, Veronique
Storme for statistical help on both the RNA-seq and lateral root density, and Martine
De Cock for help in preparing the manuscript.
article_processing_charge: No
article_type: original
author:
- first_name: Sylwia
full_name: Struk, Sylwia
last_name: Struk
- first_name: Lukas
full_name: Braem, Lukas
last_name: Braem
- first_name: Cedrick
full_name: Matthys, Cedrick
last_name: Matthys
- first_name: Alan
full_name: Walton, Alan
last_name: Walton
- first_name: Nick
full_name: Vangheluwe, Nick
last_name: Vangheluwe
- first_name: Stan
full_name: Van Praet, Stan
last_name: Van Praet
- first_name: Lingxiang
full_name: Jiang, Lingxiang
last_name: Jiang
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Carolien
full_name: De Cuyper, Carolien
last_name: De Cuyper
- first_name: Francois-Didier
full_name: Boyer, Francois-Didier
last_name: Boyer
- first_name: Elisabeth
full_name: Stes, Elisabeth
last_name: Stes
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Sofie
full_name: Goormachtig, Sofie
last_name: Goormachtig
citation:
ama: Struk S, Braem L, Matthys C, et al. Transcriptional analysis in the Arabidopsis
roots reveals new regulators that link rac-GR24 treatment with changes in flavonol
accumulation, root hair elongation and lateral root density. Plant & Cell
Physiology. 2022;63(1):104-119. doi:10.1093/pcp/pcab149
apa: Struk, S., Braem, L., Matthys, C., Walton, A., Vangheluwe, N., Van Praet, S.,
… Goormachtig, S. (2022). Transcriptional analysis in the Arabidopsis roots reveals
new regulators that link rac-GR24 treatment with changes in flavonol accumulation,
root hair elongation and lateral root density. Plant & Cell Physiology.
Oxford University Press. https://doi.org/10.1093/pcp/pcab149
chicago: Struk, Sylwia, Lukas Braem, Cedrick Matthys, Alan Walton, Nick Vangheluwe,
Stan Van Praet, Lingxiang Jiang, et al. “Transcriptional Analysis in the Arabidopsis
Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol
Accumulation, Root Hair Elongation and Lateral Root Density.” Plant & Cell
Physiology. Oxford University Press, 2022. https://doi.org/10.1093/pcp/pcab149.
ieee: S. Struk et al., “Transcriptional analysis in the Arabidopsis roots
reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation,
root hair elongation and lateral root density,” Plant & Cell Physiology,
vol. 63, no. 1. Oxford University Press, pp. 104–119, 2022.
ista: Struk S, Braem L, Matthys C, Walton A, Vangheluwe N, Van Praet S, Jiang L,
Baster P, De Cuyper C, Boyer F-D, Stes E, Beeckman T, Friml J, Gevaert K, Goormachtig
S. 2022. Transcriptional analysis in the Arabidopsis roots reveals new regulators
that link rac-GR24 treatment with changes in flavonol accumulation, root hair
elongation and lateral root density. Plant & Cell Physiology. 63(1), 104–119.
mla: Struk, Sylwia, et al. “Transcriptional Analysis in the Arabidopsis Roots Reveals
New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation,
Root Hair Elongation and Lateral Root Density.” Plant & Cell Physiology,
vol. 63, no. 1, Oxford University Press, 2022, pp. 104–19, doi:10.1093/pcp/pcab149.
short: S. Struk, L. Braem, C. Matthys, A. Walton, N. Vangheluwe, S. Van Praet, L.
Jiang, P. Baster, C. De Cuyper, F.-D. Boyer, E. Stes, T. Beeckman, J. Friml, K.
Gevaert, S. Goormachtig, Plant & Cell Physiology 63 (2022) 104–119.
date_created: 2021-12-28T11:44:18Z
date_published: 2022-01-21T00:00:00Z
date_updated: 2026-06-18T08:43:19Z
day: '21'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/pcp/pcab149
external_id:
isi:
- '000877899400009'
pmid:
- '34791413'
intvolume: ' 63'
isi: 1
issue: '1'
keyword:
- flavonols
- MAX2
- rac-Gr24
- RNA-seq
- root development
- transcriptional regulation
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1093/pcp/pcab149
month: '01'
oa: 1
oa_version: Published Version
page: 104-119
pmid: 1
publication: Plant & Cell Physiology
publication_identifier:
eissn:
- 1471-9053
issn:
- 0032-0781
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Transcriptional analysis in the Arabidopsis roots reveals new regulators that
link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation
and lateral root density
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 63
year: '2022'
...
---
_id: '10717'
abstract:
- lang: eng
text: Much of what we know about the role of auxin in plant development derives
from exogenous manipulations of auxin distribution and signaling, using inhibitors,
auxins and auxin analogs. In this context, synthetic auxin analogs, such as 1-Naphtalene
Acetic Acid (1-NAA), are often favored over the endogenous auxin indole-3-acetic
acid (IAA), in part due to their higher stability. While such auxin analogs have
proven to be instrumental to reveal the various faces of auxin, they display in
some cases distinct bioactivities compared to IAA. Here, we focused on the effect
of auxin analogs on the accumulation of PIN proteins in Brefeldin A-sensitive
endosomal aggregations (BFA bodies), and the correlation with the ability to elicit
Ca 2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin-analog
induced Ca 2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited
a Ca 2+ response, and their differential ability to elicit Ca 2+ responses correlated
partially with their ability to inhibit BFA-body formation. However, in tir1/afb
and cngc14, 1-NAA-induced Ca 2+ signaling was strongly impaired, yet 1-NAA still
could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent
Ca 2+ signaling does not inhibit BFA body formation in Arabidopsis roots.
acknowledgement: "We thank Joerg Kudla (WWU Munster, Germany), Petra Dietrich (F.A.
University of Erlangen-Nurnberg, Germany) for sharing published materials, and NASC
for providing seeds. We thank Veronique Storme for help with the statistical analyses.
Part of the imaging analysis was carried out at NOLIMITS, an advanced imaging facility
established by the University of Milan.\r\nThis work was supported by grants of
the China Scholarship Council (CSC) to RW and JC; Fonds Wetenschappelijk Onderzoek
(FWO) to TB and (G002220N) SV; the special research fund of Ghent University to
EH; the Deutsche Forschungsgemeinschaft (DFG) through Grants within FOR964 (MK and
KS); Piano di Sviluppo di Ateneo 2019 (University of Milan) to AC; the European
Research Council (ERC) T-Rex project 682436 to DVD; the ERC ETAP project 742985
to JF, and by a PhD fellowship from the University of Milan to MG."
article_number: erac019
article_processing_charge: No
article_type: original
author:
- first_name: R
full_name: Wang, R
last_name: Wang
- first_name: E
full_name: Himschoot, E
last_name: Himschoot
- first_name: M
full_name: Grenzi, M
last_name: Grenzi
- first_name: J
full_name: Chen, J
last_name: Chen
- first_name: A
full_name: Safi, A
last_name: Safi
- first_name: M
full_name: Krebs, M
last_name: Krebs
- first_name: K
full_name: Schumacher, K
last_name: Schumacher
- first_name: MK
full_name: Nowack, MK
last_name: Nowack
- first_name: W
full_name: Moeder, W
last_name: Moeder
- first_name: K
full_name: Yoshioka, K
last_name: Yoshioka
- first_name: D
full_name: Van Damme, D
last_name: Van Damme
- first_name: I
full_name: De Smet, I
last_name: De Smet
- first_name: D
full_name: Geelen, D
last_name: Geelen
- first_name: T
full_name: Beeckman, T
last_name: Beeckman
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: A
full_name: Costa, A
last_name: Costa
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
citation:
ama: Wang R, Himschoot E, Grenzi M, et al. Auxin analog-induced Ca2+ signaling is
independent of inhibition of endosomal aggregation in Arabidopsis roots. Journal
of Experimental Botany. 2022;73(8). doi:10.1093/jxb/erac019
apa: Wang, R., Himschoot, E., Grenzi, M., Chen, J., Safi, A., Krebs, M., … Vanneste,
S. (2022). Auxin analog-induced Ca2+ signaling is independent of inhibition of
endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany.
Oxford University Press. https://doi.org/10.1093/jxb/erac019
chicago: Wang, R, E Himschoot, M Grenzi, J Chen, A Safi, M Krebs, K Schumacher,
et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal
Aggregation in Arabidopsis Roots.” Journal of Experimental Botany. Oxford
University Press, 2022. https://doi.org/10.1093/jxb/erac019.
ieee: R. Wang et al., “Auxin analog-induced Ca2+ signaling is independent
of inhibition of endosomal aggregation in Arabidopsis roots,” Journal of Experimental
Botany, vol. 73, no. 8. Oxford University Press, 2022.
ista: Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack
M, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J,
Costa A, Vanneste S. 2022. Auxin analog-induced Ca2+ signaling is independent
of inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental
Botany. 73(8), erac019.
mla: Wang, R., et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition
of Endosomal Aggregation in Arabidopsis Roots.” Journal of Experimental Botany,
vol. 73, no. 8, erac019, Oxford University Press, 2022, doi:10.1093/jxb/erac019.
short: R. Wang, E. Himschoot, M. Grenzi, J. Chen, A. Safi, M. Krebs, K. Schumacher,
M. Nowack, W. Moeder, K. Yoshioka, D. Van Damme, I. De Smet, D. Geelen, T. Beeckman,
J. Friml, A. Costa, S. Vanneste, Journal of Experimental Botany 73 (2022).
date_created: 2022-02-03T09:19:01Z
date_published: 2022-04-18T00:00:00Z
date_updated: 2025-05-14T11:06:37Z
day: '18'
department:
- _id: JiFr
doi: 10.1093/jxb/erac019
ec_funded: 1
external_id:
isi:
- '000764220900001'
pmid:
- '35085386'
intvolume: ' 73'
isi: 1
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://biblio.ugent.be/publication/8738721
month: '04'
oa: 1
oa_version: Submitted Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Journal of Experimental Botany
publication_identifier:
eissn:
- 1460-2431
issn:
- 0022-0957
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal
aggregation in Arabidopsis roots
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 73
year: '2022'
...
---
_id: '10719'
abstract:
- lang: eng
text: Auxin, one of the first identified and most widely studied phytohormones,
has been and will remain a hot topic in plant biology. After more than a century
of passionate exploration, the mysteries of its synthesis, transport, signaling,
and metabolism have largely been unlocked. Due to the rapid development of new
technologies, new methods, and new genetic materials, the study of auxin has entered
the fast lane over the past 30 years. Here, we highlight advances in understanding
auxin signaling, including auxin perception, rapid auxin responses, TRANSPORT
INHIBITOR RESPONSE 1 and AUXIN SIGNALING F-boxes (TIR1/AFBs)-mediated transcriptional
and non-transcriptional branches, and the epigenetic regulation of auxin signaling.
We also focus on feedback inhibition mechanisms that prevent the over-amplification
of auxin signals. In addition, we cover the TRANSMEMBRANE KINASEs (TMKs)-mediated
non-canonical signaling, which converges with TIR1/AFBs-mediated transcriptional
regulation to coordinate plant growth and development. The identification of additional
auxin signaling components and their regulation will continue to open new avenues
of research in this field, leading to an increasingly deeper, more comprehensive
understanding of how auxin signals are interpreted at the cellular level to regulate
plant growth and development.
acknowledgement: "This research was financially supported by the National Natural
Science Foundation of China and the Israel Science Foundation (NSFC-ISF; 32061143005),
National Natural Science Foundation of China (32000225), Natural Science Foundation
of Shandong Province (ZR2020QC036), and China Postdoctoral Science Foundation (2020M682165).\r\n"
article_processing_charge: No
article_type: review
author:
- first_name: Z
full_name: Yu, Z
last_name: Yu
- first_name: F
full_name: Zhang, F
last_name: Zhang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Z
full_name: Ding, Z
last_name: Ding
citation:
ama: 'Yu Z, Zhang F, Friml J, Ding Z. Auxin signaling: Research advances over the
past 30 years. Journal of Integrative Plant Biology. 2022;64(2):371-392.
doi:10.1111/jipb.13225'
apa: 'Yu, Z., Zhang, F., Friml, J., & Ding, Z. (2022). Auxin signaling: Research
advances over the past 30 years. Journal of Integrative Plant Biology.
Wiley. https://doi.org/10.1111/jipb.13225'
chicago: 'Yu, Z, F Zhang, Jiří Friml, and Z Ding. “Auxin Signaling: Research Advances
over the Past 30 Years.” Journal of Integrative Plant Biology. Wiley, 2022.
https://doi.org/10.1111/jipb.13225.'
ieee: 'Z. Yu, F. Zhang, J. Friml, and Z. Ding, “Auxin signaling: Research advances
over the past 30 years,” Journal of Integrative Plant Biology, vol. 64,
no. 2. Wiley, pp. 371–392, 2022.'
ista: 'Yu Z, Zhang F, Friml J, Ding Z. 2022. Auxin signaling: Research advances
over the past 30 years. Journal of Integrative Plant Biology. 64(2), 371–392.'
mla: 'Yu, Z., et al. “Auxin Signaling: Research Advances over the Past 30 Years.”
Journal of Integrative Plant Biology, vol. 64, no. 2, Wiley, 2022, pp.
371–92, doi:10.1111/jipb.13225.'
short: Z. Yu, F. Zhang, J. Friml, Z. Ding, Journal of Integrative Plant Biology
64 (2022) 371–392.
corr_author: '1'
date_created: 2022-02-03T09:52:59Z
date_published: 2022-02-01T00:00:00Z
date_updated: 2026-06-18T08:47:21Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/jipb.13225
external_id:
isi:
- '000761281200011'
pmid:
- '35018726'
intvolume: ' 64'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1111/jipb.13225
month: '02'
oa: 1
oa_version: Published Version
page: 371-392
pmid: 1
publication: Journal of Integrative Plant Biology
publication_identifier:
eissn:
- 1744-7909
issn:
- 1672-9072
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Auxin signaling: Research advances over the past 30 years'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 64
year: '2022'
...
---
_id: '10768'
abstract:
- lang: eng
text: Among the most fascinated properties of the plant hormone auxin is its ability
to promote formation of its own directional transport routes. These gradually
narrowing auxin channels form from the auxin source toward the sink and involve
coordinated, collective polarization of individual cells. Once established, the
channels provide positional information, along which new vascular strands form,
for example, during organogenesis, regeneration, or leave venation. The main prerequisite
of this still mysterious auxin canalization mechanism is a feedback between auxin
signaling and its directional transport. This is manifested by auxin-induced re-arrangements
of polar, subcellular localization of PIN-FORMED (PIN) auxin exporters. Immanent
open questions relate to how position of auxin source and sink as well as tissue
context are sensed and translated into tissue polarization and how cells communicate
to polarize coordinately. Recently, identification of the first molecular players
opens new avenues into molecular studies of this intriguing example of self-organizing
plant development.
acknowledgement: The authors apologize to those researchers whose work was not cited.
In addition, exciting topics such as PIN polarization in context of phyllotaxis,
shoot branching and termination of gravitropic bending, or role of additional auxin
transporters could not have been included owing to lack of space. This work was
supported by the Czech Science Foundation GAČR (GA18-26981S). The authors also acknowledge
the EMBO for supporting J.H. with a long-term fellowship (ALTF217-2021).
article_number: '102174'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Hajny J, Tan S, Friml J. Auxin canalization: From speculative models toward
molecular players. Current Opinion in Plant Biology. 2022;65(2). doi:10.1016/j.pbi.2022.102174'
apa: 'Hajny, J., Tan, S., & Friml, J. (2022). Auxin canalization: From speculative
models toward molecular players. Current Opinion in Plant Biology. Elsevier.
https://doi.org/10.1016/j.pbi.2022.102174'
chicago: 'Hajny, Jakub, Shutang Tan, and Jiří Friml. “Auxin Canalization: From Speculative
Models toward Molecular Players.” Current Opinion in Plant Biology. Elsevier,
2022. https://doi.org/10.1016/j.pbi.2022.102174.'
ieee: 'J. Hajny, S. Tan, and J. Friml, “Auxin canalization: From speculative models
toward molecular players,” Current Opinion in Plant Biology, vol. 65, no.
2. Elsevier, 2022.'
ista: 'Hajny J, Tan S, Friml J. 2022. Auxin canalization: From speculative models
toward molecular players. Current Opinion in Plant Biology. 65(2), 102174.'
mla: 'Hajny, Jakub, et al. “Auxin Canalization: From Speculative Models toward Molecular
Players.” Current Opinion in Plant Biology, vol. 65, no. 2, 102174, Elsevier,
2022, doi:10.1016/j.pbi.2022.102174.'
short: J. Hajny, S. Tan, J. Friml, Current Opinion in Plant Biology 65 (2022).
corr_author: '1'
date_created: 2022-02-20T23:01:32Z
date_published: 2022-02-01T00:00:00Z
date_updated: 2024-10-09T21:01:37Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2022.102174
external_id:
isi:
- '000758724700004'
pmid:
- '35123880'
file:
- access_level: open_access
checksum: f1ee02b6fb4200934eeb31fa69120885
content_type: application/pdf
creator: dernst
date_created: 2022-03-10T13:34:09Z
date_updated: 2022-03-10T13:34:09Z
file_id: '10844'
file_name: 2022_CurrentOpPlantBiology_Hajny.pdf
file_size: 820322
relation: main_file
success: 1
file_date_updated: 2022-03-10T13:34:09Z
has_accepted_license: '1'
intvolume: ' 65'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Current Opinion in Plant Biology
publication_identifier:
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Auxin canalization: From speculative models toward molecular players'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 65
year: '2022'
...
---
_id: '14988'
abstract:
- lang: eng
text: Raw data generated from the publication - The TPLATE complex mediates membrane
bending during plant clathrin-mediated endocytosis by Johnson et al., 2021 In
PNAS
article_processing_charge: No
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
citation:
ama: Johnson AJ. Raw data from Johnson et al, PNAS, 2021. 2021. doi:10.5281/ZENODO.5747100
apa: Johnson, A. J. (2021). Raw data from Johnson et al, PNAS, 2021. Zenodo. https://doi.org/10.5281/ZENODO.5747100
chicago: Johnson, Alexander J. “Raw Data from Johnson et Al, PNAS, 2021.” Zenodo,
2021. https://doi.org/10.5281/ZENODO.5747100.
ieee: A. J. Johnson, “Raw data from Johnson et al, PNAS, 2021.” Zenodo, 2021.
ista: Johnson AJ. 2021. Raw data from Johnson et al, PNAS, 2021, Zenodo, 10.5281/ZENODO.5747100.
mla: Johnson, Alexander J. Raw Data from Johnson et Al, PNAS, 2021. Zenodo,
2021, doi:10.5281/ZENODO.5747100.
short: A.J. Johnson, (2021).
corr_author: '1'
date_created: 2024-02-14T14:13:48Z
date_published: 2021-12-01T00:00:00Z
date_updated: 2025-05-14T09:25:33Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.5281/ZENODO.5747100
has_accepted_license: '1'
main_file_link:
- open_access: '1'
url: https://doi.org/10.5281/zenodo.5747100
month: '12'
oa: 1
oa_version: Published Version
publisher: Zenodo
related_material:
record:
- id: '9887'
relation: used_in_publication
status: public
status: public
title: Raw data from Johnson et al, PNAS, 2021
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data_reference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2021'
...
---
_id: '15266'
abstract:
- lang: eng
text: Plant pathogens often exploit a whole range of effectors to facilitate infection.
The RXLR effector AVR1 produced by the oomycete plant pathogen Phytophthora infestans
suppresses host defense by targeting Sec5. Sec5 is a subunit of the exocyst, a
protein complex that is important for mediating polarized exocytosis during plant
development and defense against pathogens. The mechanism by which AVR1 manipulates
Sec5 functioning is unknown. In this study, we analyzed the effect of AVR1 on
Sec5 localization and functioning in the moss Physcomitrium patens. P. patens
has four Sec5 homologs. Two (PpSec5b and PpSec5d) were found to interact with
AVR1 in yeast-two-hybrid assays while none of the four showed a positive interaction
with AVR1ΔT, a truncated version of AVR1. In P. patens lines carrying β-estradiol
inducible AVR1 or AVR1ΔT transgenes, expression of AVR1 or AVR1ΔT caused defects
in the development of caulonemal protonema cells and abnormal morphology of chloronema
cells. Similar phenotypes were observed in Sec5- or Sec6-silenced P. patens lines,
suggesting that both AVR1 and AVR1ΔT affect exocyst functioning in P. patens.
With respect to Sec5 localization we found no differences between β-estradiol-treated
and untreated transgenic AVR1 lines. Sec5 localizes at the plasma membrane in
growing caulonema cells, also during pathogen attack, and its subcellular localization
is the same, with or without AVR1 in the vicinity.
article_number: e0249637
article_processing_charge: Yes
article_type: original
author:
- first_name: Elysa J. R.
full_name: Overdijk, Elysa J. R.
last_name: Overdijk
- first_name: Vera
full_name: Putker, Vera
last_name: Putker
- first_name: Joep
full_name: Smits, Joep
last_name: Smits
- first_name: Han
full_name: Tang, Han
id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E
last_name: Tang
orcid: 0000-0001-6152-6637
- first_name: Klaas
full_name: Bouwmeester, Klaas
last_name: Bouwmeester
- first_name: Francine
full_name: Govers, Francine
last_name: Govers
- first_name: Tijs
full_name: Ketelaar, Tijs
last_name: Ketelaar
citation:
ama: Overdijk EJR, Putker V, Smits J, et al. Phytophthora infestans RXLR effector
AVR1 disturbs the growth of Physcomitrium patens without affecting Sec5 localization.
PLoS One. 2021;16(4). doi:10.1371/journal.pone.0249637
apa: Overdijk, E. J. R., Putker, V., Smits, J., Tang, H., Bouwmeester, K., Govers,
F., & Ketelaar, T. (2021). Phytophthora infestans RXLR effector AVR1 disturbs
the growth of Physcomitrium patens without affecting Sec5 localization. PLoS
One. Public Library of Science. https://doi.org/10.1371/journal.pone.0249637
chicago: Overdijk, Elysa J. R., Vera Putker, Joep Smits, Han Tang, Klaas Bouwmeester,
Francine Govers, and Tijs Ketelaar. “Phytophthora Infestans RXLR Effector AVR1
Disturbs the Growth of Physcomitrium Patens without Affecting Sec5 Localization.”
PLoS One. Public Library of Science, 2021. https://doi.org/10.1371/journal.pone.0249637.
ieee: E. J. R. Overdijk et al., “Phytophthora infestans RXLR effector AVR1
disturbs the growth of Physcomitrium patens without affecting Sec5 localization,”
PLoS One, vol. 16, no. 4. Public Library of Science, 2021.
ista: Overdijk EJR, Putker V, Smits J, Tang H, Bouwmeester K, Govers F, Ketelaar
T. 2021. Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium
patens without affecting Sec5 localization. PLoS One. 16(4), e0249637.
mla: Overdijk, Elysa J. R., et al. “Phytophthora Infestans RXLR Effector AVR1 Disturbs
the Growth of Physcomitrium Patens without Affecting Sec5 Localization.” PLoS
One, vol. 16, no. 4, e0249637, Public Library of Science, 2021, doi:10.1371/journal.pone.0249637.
short: E.J.R. Overdijk, V. Putker, J. Smits, H. Tang, K. Bouwmeester, F. Govers,
T. Ketelaar, PLoS One 16 (2021).
date_created: 2024-04-03T07:38:14Z
date_published: 2021-04-08T00:00:00Z
date_updated: 2024-04-29T06:53:15Z
day: '08'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1371/journal.pone.0249637
external_id:
pmid:
- '33831039'
file:
- access_level: open_access
checksum: 25b7b329435af57db2c95571a8ef32fe
content_type: application/pdf
creator: dernst
date_created: 2024-04-29T06:51:59Z
date_updated: 2024-04-29T06:51:59Z
file_id: '15349'
file_name: 2021_PlosOne_Overdijk.pdf
file_size: 4738995
relation: main_file
success: 1
file_date_updated: 2024-04-29T06:51:59Z
has_accepted_license: '1'
intvolume: ' 16'
issue: '4'
keyword:
- Multidisciplinary
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS One
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium
patens without affecting Sec5 localization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2021'
...
---
_id: '15276'
abstract:
- lang: eng
text: Biotrophic plant pathogens secrete effector proteins to manipulate the host
physiology. Effectors suppress defenses and induce an environment favorable to
disease development. Sequence-based prediction of effector function is impeded
by their rapid evolution rate. In the maize pathogen Ustilago maydis,
effector-coding genes frequently organize in clusters. Here we describe the functional
characterization of the pleiades, a cluster of ten
effector genes, by analyzing the micro- and macroscopic phenotype of the cluster
deletion and expressing these proteins in planta. Deletion
of the pleiades leads to strongly impaired virulence
and accumulation of reactive oxygen species (ROS) in infected tissue. Eight of
the Pleiades suppress the production of ROS upon perception of pathogen associated
molecular patterns (PAMPs). Although functionally redundant, the Pleiades target
different host components. The paralogs Taygeta1 and Merope1 suppress ROS production
in either the cytoplasm or nucleus, respectively. Merope1 targets and promotes
the auto-ubiquitination activity of RFI2, a conserved family of E3 ligases that
regulates the production of PAMP-triggered ROS burst in plants.
article_number: e1009641
article_processing_charge: Yes
article_type: original
author:
- first_name: Fernando
full_name: Navarrete, Fernando
last_name: Navarrete
- first_name: Nenad
full_name: Grujic, Nenad
last_name: Grujic
- first_name: Alexandra
full_name: Stirnberg, Alexandra
last_name: Stirnberg
- first_name: Indira
full_name: Saado, Indira
last_name: Saado
- first_name: David
full_name: Aleksza, David
last_name: Aleksza
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Hazem
full_name: Adi, Hazem
last_name: Adi
- first_name: André
full_name: Alcântara, André
last_name: Alcântara
- first_name: Mamoona
full_name: Khan, Mamoona
last_name: Khan
- first_name: Janos
full_name: Bindics, Janos
last_name: Bindics
- first_name: Marco
full_name: Trujillo, Marco
last_name: Trujillo
- first_name: Armin
full_name: Djamei, Armin
last_name: Djamei
citation:
ama: Navarrete F, Grujic N, Stirnberg A, et al. The Pleiades are a cluster of fungal
effectors that inhibit host defenses. PLOS Pathogens. 2021;17(6). doi:10.1371/journal.ppat.1009641
apa: Navarrete, F., Grujic, N., Stirnberg, A., Saado, I., Aleksza, D., Gallei, M.
C., … Djamei, A. (2021). The Pleiades are a cluster of fungal effectors that inhibit
host defenses. PLOS Pathogens. Public Library of Science. https://doi.org/10.1371/journal.ppat.1009641
chicago: Navarrete, Fernando, Nenad Grujic, Alexandra Stirnberg, Indira Saado, David
Aleksza, Michelle C Gallei, Hazem Adi, et al. “The Pleiades Are a Cluster of Fungal
Effectors That Inhibit Host Defenses.” PLOS Pathogens. Public Library of
Science, 2021. https://doi.org/10.1371/journal.ppat.1009641.
ieee: F. Navarrete et al., “The Pleiades are a cluster of fungal effectors
that inhibit host defenses,” PLOS Pathogens, vol. 17, no. 6. Public Library
of Science, 2021.
ista: Navarrete F, Grujic N, Stirnberg A, Saado I, Aleksza D, Gallei MC, Adi H,
Alcântara A, Khan M, Bindics J, Trujillo M, Djamei A. 2021. The Pleiades are a
cluster of fungal effectors that inhibit host defenses. PLOS Pathogens. 17(6),
e1009641.
mla: Navarrete, Fernando, et al. “The Pleiades Are a Cluster of Fungal Effectors
That Inhibit Host Defenses.” PLOS Pathogens, vol. 17, no. 6, e1009641,
Public Library of Science, 2021, doi:10.1371/journal.ppat.1009641.
short: F. Navarrete, N. Grujic, A. Stirnberg, I. Saado, D. Aleksza, M.C. Gallei,
H. Adi, A. Alcântara, M. Khan, J. Bindics, M. Trujillo, A. Djamei, PLOS Pathogens
17 (2021).
date_created: 2024-04-03T08:00:34Z
date_published: 2021-06-24T00:00:00Z
date_updated: 2024-04-09T10:26:12Z
day: '24'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1371/journal.ppat.1009641
external_id:
pmid:
- '34166468'
file:
- access_level: open_access
checksum: ab8428291a0c14607c4ea5656c029cff
content_type: application/pdf
creator: dernst
date_created: 2024-04-09T10:24:43Z
date_updated: 2024-04-09T10:24:43Z
file_id: '15305'
file_name: 2021_PlosPathogens_Navarrete.pdf
file_size: 2616563
relation: main_file
success: 1
file_date_updated: 2024-04-09T10:24:43Z
has_accepted_license: '1'
intvolume: ' 17'
issue: '6'
keyword:
- Virology
- Genetics
- Molecular Biology
- Immunology
- Microbiology
- Parasitology
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLOS Pathogens
publication_identifier:
issn:
- 1553-7374
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: The Pleiades are a cluster of fungal effectors that inhibit host defenses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2021'
...
---
_id: '10223'
abstract:
- lang: eng
text: Growth regulation tailors development in plants to their environment. A prominent
example of this is the response to gravity, in which shoots bend up and roots
bend down1. This paradox is based on opposite effects of the phytohormone auxin,
which promotes cell expansion in shoots while inhibiting it in roots via a yet
unknown cellular mechanism2. Here, by combining microfluidics, live imaging, genetic
engineering and phosphoproteomics in Arabidopsis thaliana, we advance understanding
of how auxin inhibits root growth. We show that auxin activates two distinct,
antagonistically acting signalling pathways that converge on rapid regulation
of apoplastic pH, a causative determinant of growth. Cell surface-based TRANSMEMBRANE
KINASE1 (TMK1) interacts with and mediates phosphorylation and activation of plasma
membrane H+-ATPases for apoplast acidification, while intracellular canonical
auxin signalling promotes net cellular H+ influx, causing apoplast alkalinization.
Simultaneous activation of these two counteracting mechanisms poises roots for
rapid, fine-tuned growth modulation in navigating complex soil environments.
acknowledged_ssus:
- _id: LifeSc
- _id: M-Shop
- _id: Bio
acknowledgement: We thank N. Gnyliukh and L. Hörmayer for technical assistance and
N. Paris for sharing PM-Cyto seeds. We gratefully acknowledge the Life Science,
Machine Shop and Bioimaging Facilities of IST Austria. This project has received
funding from the European Research Council Advanced Grant (ETAP-742985) and the
Austrian Science Fund (FWF) under I 3630-B25 to J.F., the National Institutes of
Health (GM067203) to W.M.G., the Netherlands Organization for Scientific Research
(NWO; VIDI-864.13.001), Research Foundation-Flanders (FWO; Odysseus II G0D0515N)
and a European Research Council Starting Grant (TORPEDO-714055) to W.S. and B.D.R.,
the VICI grant (865.14.001) from the Netherlands Organization for Scientific Research
to M.R. and D.W., the Australian Research Council and China National Distinguished
Expert Project (WQ20174400441) to S.S., the MEXT/JSPS KAKENHI to K.T. (20K06685)
and T.K. (20H05687 and 20H05910), the European Union’s Horizon 2020 research and
innovation programme under Marie Skłodowska-Curie grant agreement no. 665385 and
the DOC Fellowship of the Austrian Academy of Sciences to L.L., and the China Scholarship
Council to J.C.
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Jian
full_name: Chen, Jian
last_name: Chen
- first_name: Lana
full_name: Shabala, Lana
last_name: Shabala
- first_name: Wouter
full_name: Smet, Wouter
last_name: Smet
- first_name: Hong
full_name: Ren, Hong
last_name: Ren
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Sergey
full_name: Shabala, Sergey
last_name: Shabala
- first_name: Bert
full_name: De Rybel, Bert
last_name: De Rybel
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Toshinori
full_name: Kinoshita, Toshinori
last_name: Kinoshita
- first_name: William M.
full_name: Gray, William M.
last_name: Gray
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Verstraeten I, Roosjen M, et al. Cell surface and intracellular auxin
signalling for H+ fluxes in root growth. Nature. 2021;599(7884):273-277.
doi:10.1038/s41586-021-04037-6
apa: Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez Solovey, L.,
Merrin, J., … Friml, J. (2021). Cell surface and intracellular auxin signalling
for H+ fluxes in root growth. Nature. Springer Nature. https://doi.org/10.1038/s41586-021-04037-6
chicago: Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez
Solovey, Jack Merrin, Jian Chen, et al. “Cell Surface and Intracellular Auxin
Signalling for H+ Fluxes in Root Growth.” Nature. Springer Nature,
2021. https://doi.org/10.1038/s41586-021-04037-6.
ieee: L. Li et al., “Cell surface and intracellular auxin signalling for
H+ fluxes in root growth,” Nature, vol. 599, no. 7884. Springer
Nature, pp. 273–277, 2021.
ista: Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez Solovey L, Merrin J,
Chen J, Shabala L, Smet W, Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D,
Kinoshita T, Gray WM, Friml J. 2021. Cell surface and intracellular auxin signalling
for H+ fluxes in root growth. Nature. 599(7884), 273–277.
mla: Li, Lanxin, et al. “Cell Surface and Intracellular Auxin Signalling for H+
Fluxes in Root Growth.” Nature, vol. 599, no. 7884, Springer Nature, 2021,
pp. 273–77, doi:10.1038/s41586-021-04037-6.
short: L. Li, I. Verstraeten, M. Roosjen, K. Takahashi, L. Rodriguez Solovey, J.
Merrin, J. Chen, L. Shabala, W. Smet, H. Ren, S. Vanneste, S. Shabala, B. De Rybel,
D. Weijers, T. Kinoshita, W.M. Gray, J. Friml, Nature 599 (2021) 273–277.
corr_author: '1'
date_created: 2021-11-07T23:01:25Z
date_published: 2021-11-11T00:00:00Z
date_updated: 2025-07-10T11:49:46Z
day: '11'
department:
- _id: JiFr
- _id: NanoFab
doi: 10.1038/s41586-021-04037-6
ec_funded: 1
external_id:
isi:
- '000713338100006'
pmid:
- '34707283'
intvolume: ' 599'
isi: 1
issue: '7884'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.doi.org/10.21203/rs.3.rs-266395/v3
month: '11'
oa: 1
oa_version: Preprint
page: 273-277
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Webpage
relation: press_release
url: https://ist.ac.at/en/news/stop-and-grow/
record:
- id: '10095'
relation: earlier_version
status: public
scopus_import: '1'
status: public
title: Cell surface and intracellular auxin signalling for H+ fluxes in
root growth
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 599
year: '2021'
...
---
_id: '10267'
abstract:
- lang: eng
text: Tropisms are among the most important growth responses for plant adaptation
to the surrounding environment. One of the most common tropisms is root gravitropism.
Root gravitropism enables the plant to anchor securely to the soil enabling the
absorption of water and nutrients. Most of the knowledge related to the plant
gravitropism has been acquired from the flowering plants, due to limited research
in non-seed plants. Limited research on non-seed plants is due in large part to
the lack of standard research methods. Here, we describe the experimental methods
to evaluate gravitropism in representative non-seed plant species, including the
non-vascular plant moss Physcomitrium patens, the early diverging extant vascular
plant lycophyte Selaginella moellendorffii and fern Ceratopteris richardii. In
addition, we introduce the methods used for statistical analysis of the root gravitropism
in non-seed plant species.
acknowledgement: The Ceratopteris richardii spores were obtained from the lab of Jo
Ann Banks at Purdue University. This work was supported by funding from the European
Union’s Horizon 2020 research and innovation program (ERC grant agreement number
742985), Austrian Science Fund (FWF, grant number I 3630-B25), IST Fellow program
and DOC Fellowship of the Austrian Academy of Sciences.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Zhang Y, Li L, Friml J. Evaluation of gravitropism in non-seed plants. In:
Blancaflor EB, ed. Plant Gravitropism. Vol 2368. MIMB. Springer Nature;
2021:43-51. doi:10.1007/978-1-0716-1677-2_2'
apa: Zhang, Y., Li, L., & Friml, J. (2021). Evaluation of gravitropism in non-seed
plants. In E. B. Blancaflor (Ed.), Plant Gravitropism (Vol. 2368, pp. 43–51).
Springer Nature. https://doi.org/10.1007/978-1-0716-1677-2_2
chicago: Zhang, Yuzhou, Lanxin Li, and Jiří Friml. “Evaluation of Gravitropism in
Non-Seed Plants.” In Plant Gravitropism, edited by Elison B Blancaflor,
2368:43–51. MIMB. Springer Nature, 2021. https://doi.org/10.1007/978-1-0716-1677-2_2.
ieee: Y. Zhang, L. Li, and J. Friml, “Evaluation of gravitropism in non-seed plants,”
in Plant Gravitropism, vol. 2368, E. B. Blancaflor, Ed. Springer Nature,
2021, pp. 43–51.
ista: 'Zhang Y, Li L, Friml J. 2021.Evaluation of gravitropism in non-seed plants.
In: Plant Gravitropism. Methods in Molecular Biology, vol. 2368, 43–51.'
mla: Zhang, Yuzhou, et al. “Evaluation of Gravitropism in Non-Seed Plants.” Plant
Gravitropism, edited by Elison B Blancaflor, vol. 2368, Springer Nature, 2021,
pp. 43–51, doi:10.1007/978-1-0716-1677-2_2.
short: Y. Zhang, L. Li, J. Friml, in:, E.B. Blancaflor (Ed.), Plant Gravitropism,
Springer Nature, 2021, pp. 43–51.
corr_author: '1'
date_created: 2021-11-11T09:26:10Z
date_published: 2021-10-14T00:00:00Z
date_updated: 2025-04-14T07:45:00Z
day: '14'
department:
- _id: JiFr
doi: 10.1007/978-1-0716-1677-2_2
ec_funded: 1
editor:
- first_name: Elison B
full_name: Blancaflor, Elison B
last_name: Blancaflor
external_id:
pmid:
- '34647246'
intvolume: ' 2368'
language:
- iso: eng
month: '10'
oa_version: None
page: 43-51
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Gravitropism
publication_identifier:
eisbn:
- 978-1-0716-1677-2
isbn:
- 978-1-0716-1676-5
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Evaluation of gravitropism in non-seed plants
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2368
year: '2021'
...
---
_id: '10268'
abstract:
- lang: eng
text: The analysis of dynamic cellular processes such as plant cytokinesis stands
and falls with live-cell time-lapse confocal imaging. Conventional approaches
to time-lapse imaging of cell division in Arabidopsis root tips are tedious and
have low throughput. Here, we describe a protocol for long-term time-lapse simultaneous
imaging of multiple root tips on a vertical-stage confocal microscope with automated
root tracking. We also provide modifications of the basic protocol to implement
this imaging method in the analysis of genetic, pharmacological or laser ablation
wounding-mediated experimental manipulations. Our method dramatically improves
the efficiency of cell division time-lapse imaging by increasing the throughput,
while reducing the person-hour requirements of such experiments.
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank B. De Rybel for allowing M.G. to work on this manuscript
during a postdoc in his laboratory, and EMBO for supporting M.G. with a Long-Term
fellowship (ALTF 1005-2019) during this time. We acknowledge the service and support
by the Bioimaging Facility at IST Austria, and finally, we thank A. Mally for proofreading
and correcting the manuscript.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
citation:
ama: 'Hörmayer L, Friml J, Glanc M. Automated time-lapse imaging and manipulation
of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy.
In: Plant Cell Division. Vol 2382. MIMB. Humana Press; 2021:105-114. doi:10.1007/978-1-0716-1744-1_6'
apa: Hörmayer, L., Friml, J., & Glanc, M. (2021). Automated time-lapse imaging
and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal
microscopy. In Plant Cell Division (Vol. 2382, pp. 105–114). Humana Press.
https://doi.org/10.1007/978-1-0716-1744-1_6
chicago: Hörmayer, Lukas, Jiří Friml, and Matous Glanc. “Automated Time-Lapse Imaging
and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal
Microscopy.” In Plant Cell Division, 2382:105–14. MIMB. Humana Press, 2021.
https://doi.org/10.1007/978-1-0716-1744-1_6.
ieee: L. Hörmayer, J. Friml, and M. Glanc, “Automated time-lapse imaging and manipulation
of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy,”
in Plant Cell Division, vol. 2382, Humana Press, 2021, pp. 105–114.
ista: 'Hörmayer L, Friml J, Glanc M. 2021.Automated time-lapse imaging and manipulation
of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy.
In: Plant Cell Division. Methods in Molecular Biology, vol. 2382, 105–114.'
mla: Hörmayer, Lukas, et al. “Automated Time-Lapse Imaging and Manipulation of Cell
Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy.” Plant
Cell Division, vol. 2382, Humana Press, 2021, pp. 105–14, doi:10.1007/978-1-0716-1744-1_6.
short: L. Hörmayer, J. Friml, M. Glanc, in:, Plant Cell Division, Humana Press,
2021, pp. 105–114.
date_created: 2021-11-11T10:03:30Z
date_published: 2021-10-28T00:00:00Z
date_updated: 2022-06-03T06:47:06Z
day: '28'
department:
- _id: JiFr
doi: 10.1007/978-1-0716-1744-1_6
external_id:
pmid:
- '34705235'
intvolume: ' 2382'
language:
- iso: eng
month: '10'
oa_version: None
page: 105-114
pmid: 1
publication: Plant Cell Division
publication_identifier:
eisbn:
- 978-1-0716-1744-1
eissn:
- 1940-6029
isbn:
- 978-1-0716-1743-4
issn:
- 1064-3745
publication_status: published
publisher: Humana Press
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis
roots by vertical-stage confocal microscopy
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2382
year: '2021'
...
---
OA_place: repository
OA_type: green
_id: '10326'
abstract:
- lang: eng
text: Strigolactones (SLs) are carotenoid-derived plant hormones that control shoot
branching and communications between host plants and symbiotic fungi or root parasitic
plants. Extensive studies have identified the key components participating in
SL biosynthesis and signalling, whereas the catabolism or deactivation of endogenous
SLs in planta remains largely unknown. Here, we report that the Arabidopsis carboxylesterase
15 (AtCXE15) and its orthologues function as efficient hydrolases of SLs. We show
that overexpression of AtCXE15 promotes shoot branching by dampening SL-inhibited
axillary bud outgrowth. We further demonstrate that AtCXE15 could bind and efficiently
hydrolyse SLs both in vitro and in planta. We also provide evidence that AtCXE15
is capable of catalysing hydrolysis of diverse SL analogues and that such CXE15-dependent
catabolism of SLs is evolutionarily conserved in seed plants. These results disclose
a catalytic mechanism underlying homoeostatic regulation of SLs in plants, which
also provides a rational approach to spatial-temporally manipulate the endogenous
SLs and thus architecture of crops and ornamental plants.
acknowledgement: We thank J. Li (Institute of Genetics and Developmental Biology,
China) for providing the at14-1, atmax2-1, atmax3-9, atmax4-1, atmax1-1, kai2-2
(Col-0 background) mutants and B. Xu for providing the complementary DNA of P. patens.
We are grateful to L. Wang for assistance with MST, B. Han for assistance with UPLC–MS,
J. Li for assistance with confocal microscopy and B. Mikael and J. Zhang for their
comments on the manuscript. This work was supported by grants from Strategic Priority
Research Program of Chinese Academy of Sciences (Y.H., XDB27030102) and the National
Natural Science Foundation of China (E.X., 31700253; Y.H., 31830055).
article_processing_charge: No
article_type: original
author:
- first_name: Enjun
full_name: Xu, Enjun
last_name: Xu
- first_name: Liang
full_name: Chai, Liang
last_name: Chai
- first_name: Shiqi
full_name: Zhang, Shiqi
last_name: Zhang
- first_name: Ruixue
full_name: Yu, Ruixue
last_name: Yu
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Chongyi
full_name: Xu, Chongyi
last_name: Xu
- first_name: Yuxin
full_name: Hu, Yuxin
last_name: Hu
citation:
ama: Xu E, Chai L, Zhang S, et al. Catabolism of strigolactones by a carboxylesterase.
Nature Plants. 2021;7:1495–1504. doi:10.1038/s41477-021-01011-y
apa: Xu, E., Chai, L., Zhang, S., Yu, R., Zhang, X., Xu, C., & Hu, Y. (2021).
Catabolism of strigolactones by a carboxylesterase. Nature Plants. Springer
Nature. https://doi.org/10.1038/s41477-021-01011-y
chicago: Xu, Enjun, Liang Chai, Shiqi Zhang, Ruixue Yu, Xixi Zhang, Chongyi Xu,
and Yuxin Hu. “Catabolism of Strigolactones by a Carboxylesterase.” Nature
Plants. Springer Nature, 2021. https://doi.org/10.1038/s41477-021-01011-y.
ieee: E. Xu et al., “Catabolism of strigolactones by a carboxylesterase,”
Nature Plants, vol. 7. Springer Nature, pp. 1495–1504, 2021.
ista: Xu E, Chai L, Zhang S, Yu R, Zhang X, Xu C, Hu Y. 2021. Catabolism of strigolactones
by a carboxylesterase. Nature Plants. 7, 1495–1504.
mla: Xu, Enjun, et al. “Catabolism of Strigolactones by a Carboxylesterase.” Nature
Plants, vol. 7, Springer Nature, 2021, pp. 1495–1504, doi:10.1038/s41477-021-01011-y.
short: E. Xu, L. Chai, S. Zhang, R. Yu, X. Zhang, C. Xu, Y. Hu, Nature Plants 7
(2021) 1495–1504.
date_created: 2021-11-21T23:01:30Z
date_published: 2021-11-11T00:00:00Z
date_updated: 2025-01-21T12:42:52Z
day: '11'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-021-01011-y
external_id:
isi:
- '000717408000002'
pmid:
- '34764442'
file:
- access_level: open_access
checksum: d20231806bea67f0fd19e96a94a048f4
content_type: application/pdf
creator: dernst
date_created: 2025-01-21T12:41:43Z
date_updated: 2025-01-21T12:41:43Z
file_id: '18864'
file_name: Accepted version_Xu et al.,2021 Catabolism of strigolactones by a carboxylesterase.pdf
file_size: 41109943
relation: main_file
success: 1
file_date_updated: 2025-01-21T12:41:43Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Submitted Version
page: '1495–1504 '
pmid: 1
publication: Nature Plants
publication_identifier:
eissn:
- 2055-0278
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Catabolism of strigolactones by a carboxylesterase
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2021'
...
---
_id: '8582'
abstract:
- lang: eng
text: "Cell and tissue polarization is fundamental for plant growth and morphogenesis.
The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial
for their function in directional auxin transport. The clustering of PIN polar
cargoes within the plasma membrane has been proposed to be important for the maintenance
of their polar distribution. However, the more detailed features of PIN clusters
and the cellular requirements of cargo clustering remain unclear.\r\nHere, we
characterized PIN clusters in detail by means of multiple advanced microscopy
and quantification methods, such as 3D quantitative imaging or freeze‐fracture
replica labeling. The size and aggregation types of PIN clusters were determined
by electron microscopy at the nanometer level at different polar domains and at
different developmental stages, revealing a strong preference for clustering at
the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters
depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall
components as well as connections between the cell wall and the plasma membrane.\r\nThis
study identifies the role of different cellular processes and structures in polar
cargo clustering and provides initial mechanistic insight into the maintenance
of polarity in plants and other systems."
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg)
for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1
mutant and the complementation lines, and Dr Patricia C. Zambryski (University of
California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria)
for assistance with imaging, group members for discussions, Martine De Cock for
help in preparing the manuscript and Nataliia Gnyliukh for critical reading and
revision of the manuscript. This project received funding from the European Research
Council (ERC) under the European Union's Horizon 2020 research and innovation program
(grant agreement No. 742985) and Comisión Nacional de Investigación Científica y
Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People
Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme
(FP7/2007‐2013) under REA grant agreement no. 291734.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Hongjiang
full_name: Li, Hongjiang
id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0001-5039-9660
- first_name: Daniel
full_name: von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Nasser
full_name: Darwish-Miranda, Nasser
id: 39CD9926-F248-11E8-B48F-1D18A9856A87
last_name: Darwish-Miranda
orcid: 0000-0002-8821-8236
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Riet
full_name: de Rycke, Riet
last_name: de Rycke
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Daniel J
full_name: Gütl, Daniel J
id: 381929CE-F248-11E8-B48F-1D18A9856A87
last_name: Gütl
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Meiyu
full_name: Ke, Meiyu
last_name: Ke
- first_name: Xu
full_name: Chen, Xu
id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Jan
full_name: Dettmer, Jan
last_name: Dettmer
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar
cargo clustering in Arabidopsis thaliana. New Phytologist. 2021;229(1):351-369.
doi:10.1111/nph.16887
apa: Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto,
S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in
Arabidopsis thaliana. New Phytologist. Wiley. https://doi.org/10.1111/nph.16887
chicago: Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda,
Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar
Cargo Clustering in Arabidopsis Thaliana.” New Phytologist. Wiley, 2021.
https://doi.org/10.1111/nph.16887.
ieee: H. Li et al., “Cellular requirements for PIN polar cargo clustering
in Arabidopsis thaliana,” New Phytologist, vol. 229, no. 1. Wiley, pp.
351–369, 2021.
ista: Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik
KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer
J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis
thaliana. New Phytologist. 229(1), 351–369.
mla: Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering
in Arabidopsis Thaliana.” New Phytologist, vol. 229, no. 1, Wiley, 2021,
pp. 351–69, doi:10.1111/nph.16887.
short: H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto,
K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke,
X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.
date_created: 2020-09-28T08:59:28Z
date_published: 2021-01-01T00:00:00Z
date_updated: 2025-06-12T06:32:24Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: EM-Fac
- _id: Bio
- _id: EvBe
doi: 10.1111/nph.16887
ec_funded: 1
external_id:
isi:
- '000570187900001'
pmid:
- '32810889'
file:
- access_level: open_access
checksum: b45621607b4cab97eeb1605ab58e896e
content_type: application/pdf
creator: dernst
date_created: 2021-02-04T09:44:17Z
date_updated: 2021-02-04T09:44:17Z
file_id: '9084'
file_name: 2021_NewPhytologist_Li.pdf
file_size: 4061962
relation: main_file
success: 1
file_date_updated: 2021-02-04T09:44:17Z
has_accepted_license: '1'
intvolume: ' 229'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 351-369
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 229
year: '2021'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '8606'
abstract:
- lang: eng
text: The leaf is a crucial organ evolved with remarkable morphological diversity
to maximize plant photosynthesis. The leaf shape is a key trait that affects photosynthesis,
flowering rates, disease resistance, and yield. Although many genes regulating
leaf development have been identified in the past years, the precise regulatory
architecture underlying the generation of diverse leaf shapes remains to be elucidated.
We used cotton as a reference model to probe the genetic framework underlying
divergent leaf forms. Comparative transcriptome analysis revealed that the GhARF16‐1
and GhKNOX2‐1 genes might be potential regulators of leaf shape. We functionally
characterized the auxin‐responsive factor ARF16‐1 acting upstream of GhKNOX2‐1
to determine leaf morphology in cotton. The transcription of GhARF16‐1 was significantly
higher in lobed‐leaved cotton than in smooth‐leaved cotton. Furthermore, the overexpression
of GhARF16‐1 led to the upregulation of GhKNOX2‐1 and resulted in more and deeper
serrations in cotton leaves, similar to the leaf shape of cotton plants overexpressing
GhKNOX2‐1. We found that GhARF16‐1 specifically bound to the promoter of GhKNOX2‐1
to induce its expression. The heterologous expression of GhARF16‐1 and GhKNOX2‐1
in Arabidopsis led to lobed and curly leaves, and a genetic analysis revealed
that GhKNOX2‐1 is epistatic to GhARF16‐1 in Arabidopsis, suggesting that the GhARF16‐1
and GhKNOX2‐1 interaction paradigm also functions to regulate leaf shape in Arabidopsis.
To our knowledge, our results uncover a novel mechanism by which auxin, through
the key component ARF16‐1 and its downstream‐activated gene KNOX2‐1, determines
leaf morphology in eudicots.
acknowledgement: We are thankful to Professor Yuxian Zhu from Wuhan University for
his extremely valuable remarks and helpful comments on the manuscript. This work
was supported by the Shaanxi Natural Science Foundation (2019JQ‐062 and 2020JQ‐410),
Shaanxi Youth Entrusted Talents Program (20190205), China Postdoctoral Science Foundation
(2018M640947, 2020T130394), Shaanxi Postdoctoral Project (2018BSHYDZZ76), Natural
Science Basic Research Plan in Shaanxi Province of China (2018JZ3006), Fundamental
Research Funds for the Central Universities (GK201903064, GK201901004, GK202002005
and GK202001004), and State Key Laboratory of Cotton Biology Open Fund (CB2020A12).
article_processing_charge: No
article_type: original
author:
- first_name: P
full_name: He, P
last_name: He
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: H
full_name: Li, H
last_name: Li
- first_name: X
full_name: Fu, X
last_name: Fu
- first_name: H
full_name: Shang, H
last_name: Shang
- first_name: C
full_name: Zou, C
last_name: Zou
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Xiao, G
last_name: Xiao
citation:
ama: He P, Zhang Y, Li H, et al. GhARF16-1 modulates leaf development by transcriptionally
regulating the GhKNOX2-1 gene in cotton. Plant Biotechnology Journal. 2021;19(3):548-562.
doi:10.1111/pbi.13484
apa: He, P., Zhang, Y., Li, H., Fu, X., Shang, H., Zou, C., … Xiao, G. (2021). GhARF16-1
modulates leaf development by transcriptionally regulating the GhKNOX2-1 gene
in cotton. Plant Biotechnology Journal. Wiley. https://doi.org/10.1111/pbi.13484
chicago: He, P, Yuzhou Zhang, H Li, X Fu, H Shang, C Zou, Jiří Friml, and G Xiao.
“GhARF16-1 Modulates Leaf Development by Transcriptionally Regulating the GhKNOX2-1
Gene in Cotton.” Plant Biotechnology Journal. Wiley, 2021. https://doi.org/10.1111/pbi.13484.
ieee: P. He et al., “GhARF16-1 modulates leaf development by transcriptionally
regulating the GhKNOX2-1 gene in cotton,” Plant Biotechnology Journal,
vol. 19, no. 3. Wiley, pp. 548–562, 2021.
ista: He P, Zhang Y, Li H, Fu X, Shang H, Zou C, Friml J, Xiao G. 2021. GhARF16-1
modulates leaf development by transcriptionally regulating the GhKNOX2-1 gene
in cotton. Plant Biotechnology Journal. 19(3), 548–562.
mla: He, P., et al. “GhARF16-1 Modulates Leaf Development by Transcriptionally Regulating
the GhKNOX2-1 Gene in Cotton.” Plant Biotechnology Journal, vol. 19, no.
3, Wiley, 2021, pp. 548–62, doi:10.1111/pbi.13484.
short: P. He, Y. Zhang, H. Li, X. Fu, H. Shang, C. Zou, J. Friml, G. Xiao, Plant
Biotechnology Journal 19 (2021) 548–562.
date_created: 2020-10-05T12:44:33Z
date_published: 2021-03-01T00:00:00Z
date_updated: 2025-07-10T11:57:13Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/pbi.13484
external_id:
isi:
- '000577682300001'
pmid:
- '32981232'
file:
- access_level: open_access
checksum: 63845be37fb962586e0c7773f2355970
content_type: application/pdf
creator: dernst
date_created: 2021-04-12T12:29:07Z
date_updated: 2021-04-12T12:29:07Z
file_id: '9321'
file_name: 2021_PlantBiotechJournal_He.pdf
file_size: 15691871
relation: main_file
success: 1
file_date_updated: 2021-04-12T12:29:07Z
has_accepted_license: '1'
intvolume: ' 19'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 548-562
pmid: 1
publication: Plant Biotechnology Journal
publication_identifier:
eissn:
- 1467-7652
issn:
- 1467-7644
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: GhARF16-1 modulates leaf development by transcriptionally regulating the GhKNOX2-1
gene in cotton
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 19
year: '2021'
...
---
_id: '8608'
abstract:
- lang: eng
text: To adapt to the diverse array of biotic and abiotic cues, plants have evolved
sophisticated mechanisms to sense changes in environmental conditions and modulate
their growth. Growth-promoting hormones and defence signalling fine tune plant
development antagonistically. During host-pathogen interactions, this defence-growth
trade-off is mediated by the counteractive effects of the defence hormone salicylic
acid (SA) and the growth hormone auxin. Here we revealed an underlying mechanism
of SA regulating auxin signalling by constraining the plasma membrane dynamics
of PIN2 auxin efflux transporter in Arabidopsis thaliana roots. The lateral diffusion
of PIN2 proteins is constrained by SA signalling, during which PIN2 proteins are
condensed into hyperclusters depending on REM1.2-mediated nanodomain compartmentalisation.
Furthermore, membrane nanodomain compartmentalisation by SA or Remorin (REM) assembly
significantly suppressed clathrin-mediated endocytosis. Consequently, SA-induced
heterogeneous surface condensation disrupted asymmetric auxin distribution and
the resultant gravitropic response. Our results demonstrated a defence-growth
trade-off mechanism by which SA signalling crosstalked with auxin transport by
concentrating membrane-resident PIN2 into heterogeneous compartments.
acknowledgement: This work was supported by the National Key Research andDevelopment
Programme of China (2017YFA0506100), theNational Natural Science Foundation of China
(31870170 and31701168), and the Fok Ying Tung Education Foundation(161027) to XC;
NTU startup grant (M4081533) and NIM/01/2016 (NTU, Singapore) to YM. We thank Lei
Shi andZhongquan Lin for microscopy assistance.
article_processing_charge: No
article_type: original
author:
- first_name: M
full_name: Ke, M
last_name: Ke
- first_name: Z
full_name: Ma, Z
last_name: Ma
- first_name: D
full_name: Wang, D
last_name: Wang
- first_name: Y
full_name: Sun, Y
last_name: Sun
- first_name: C
full_name: Wen, C
last_name: Wen
- first_name: D
full_name: Huang, D
last_name: Huang
- first_name: Z
full_name: Chen, Z
last_name: Chen
- first_name: L
full_name: Yang, L
last_name: Yang
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: R
full_name: Li, R
last_name: Li
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Y
full_name: Miao, Y
last_name: Miao
- first_name: X
full_name: Chen, X
last_name: Chen
citation:
ama: Ke M, Ma Z, Wang D, et al. Salicylic acid regulates PIN2 auxin transporter
hyper-clustering and root gravitropic growth via Remorin-dependent lipid nanodomain
organization in Arabidopsis thaliana. New Phytologist. 2021;229(2):963-978.
doi:10.1111/nph.16915
apa: Ke, M., Ma, Z., Wang, D., Sun, Y., Wen, C., Huang, D., … Chen, X. (2021). Salicylic
acid regulates PIN2 auxin transporter hyper-clustering and root gravitropic growth
via Remorin-dependent lipid nanodomain organization in Arabidopsis thaliana. New
Phytologist. Wiley. https://doi.org/10.1111/nph.16915
chicago: Ke, M, Z Ma, D Wang, Y Sun, C Wen, D Huang, Z Chen, et al. “Salicylic Acid
Regulates PIN2 Auxin Transporter Hyper-Clustering and Root Gravitropic Growth
via Remorin-Dependent Lipid Nanodomain Organization in Arabidopsis Thaliana.”
New Phytologist. Wiley, 2021. https://doi.org/10.1111/nph.16915.
ieee: M. Ke et al., “Salicylic acid regulates PIN2 auxin transporter hyper-clustering
and root gravitropic growth via Remorin-dependent lipid nanodomain organization
in Arabidopsis thaliana,” New Phytologist, vol. 229, no. 2. Wiley, pp.
963–978, 2021.
ista: Ke M, Ma Z, Wang D, Sun Y, Wen C, Huang D, Chen Z, Yang L, Tan S, Li R, Friml
J, Miao Y, Chen X. 2021. Salicylic acid regulates PIN2 auxin transporter hyper-clustering
and root gravitropic growth via Remorin-dependent lipid nanodomain organization
in Arabidopsis thaliana. New Phytologist. 229(2), 963–978.
mla: Ke, M., et al. “Salicylic Acid Regulates PIN2 Auxin Transporter Hyper-Clustering
and Root Gravitropic Growth via Remorin-Dependent Lipid Nanodomain Organization
in Arabidopsis Thaliana.” New Phytologist, vol. 229, no. 2, Wiley, 2021,
pp. 963–78, doi:10.1111/nph.16915.
short: M. Ke, Z. Ma, D. Wang, Y. Sun, C. Wen, D. Huang, Z. Chen, L. Yang, S. Tan,
R. Li, J. Friml, Y. Miao, X. Chen, New Phytologist 229 (2021) 963–978.
date_created: 2020-10-05T12:45:36Z
date_published: 2021-01-01T00:00:00Z
date_updated: 2023-09-05T16:06:24Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.16915
external_id:
isi:
- '000573568000001'
pmid:
- '32901934'
file:
- access_level: open_access
checksum: d36b6a8c6fafab66264e0d27114dae63
content_type: application/pdf
creator: dernst
date_created: 2021-02-04T09:53:16Z
date_updated: 2021-02-04T09:53:16Z
file_id: '9085'
file_name: 2021_NewPhytologist_Ke.pdf
file_size: 3674502
relation: main_file
success: 1
file_date_updated: 2021-02-04T09:53:16Z
has_accepted_license: '1'
intvolume: ' 229'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 963-978
pmid: 1
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Salicylic acid regulates PIN2 auxin transporter hyper-clustering and root gravitropic
growth via Remorin-dependent lipid nanodomain organization in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 229
year: '2021'
...
---
_id: '8824'
abstract:
- lang: eng
text: Plants are able to orient their growth according to gravity, which ultimately
controls both shoot and root architecture.1 Gravitropism is a dynamic process
whereby gravistimulation induces the asymmetric distribution of the plant hormone
auxin, leading to asymmetric growth, organ bending, and subsequent reset of auxin
distribution back to the original pre-gravistimulation situation.1, 2, 3 Differential
auxin accumulation during the gravitropic response depends on the activity of
polarly localized PIN-FORMED (PIN) auxin-efflux carriers.1, 2, 3, 4 In particular,
the timing of this dynamic response is regulated by PIN2,5,6 but the underlying
molecular mechanisms are poorly understood. Here, we show that MEMBRANE ASSOCIATED
KINASE REGULATOR2 (MAKR2) controls the pace of the root gravitropic response.
We found that MAKR2 is required for the PIN2 asymmetry during gravitropism by
acting as a negative regulator of the cell-surface signaling mediated by the receptor-like
kinase TRANSMEMBRANE KINASE1 (TMK1).2,7, 8, 9, 10 Furthermore, we show that
the MAKR2 inhibitory effect on TMK1 signaling is antagonized by auxin itself,
which triggers rapid MAKR2 membrane dissociation in a TMK1-dependent manner. Our
findings suggest that the timing of the root gravitropic response is orchestrated
by the reversible inhibition of the TMK1 signaling pathway at the cell surface.
acknowledgement: "We thank the SiCE group for discussions and comments; S. Yalovsky,
B. Scheres, and the NASC/ABRC collection for providing transgenic Arabidopsis lines
and plasmids; L. Kalmbach and M. Barberon for the gift of pLOK180_pFR7m34GW; A.
Lacroix, J. Berger, and P. Bolland for plant care; and M. Fendrych for help with
microfluidics in the J.F. lab. We acknowledge\r\nthe contribution of the SFR Biosciences
(UMS3444/CNRS, US8/Inser m, ENS de Lyon, UCBL) facilities: C. Lionet, E. Chatre,
and J. Brocard at LBIPLATIM-MICROSCOPY for assistance with imaging, and V. GuegenChaignon
and A. Page at the Protein Science Facility (PSF) for assistance with protein purification
and mass spectrometry. Y.J. was funded by ERC\r\ngrant 3363360-APPL under FP/2007–2013.
Y.J. and Z.L.N. were funded by an ANR- and NSF-supported ERA-CAPS project (SICOPID:
ANR-17-CAPS0003-01/NSF PGRP IOS-1841917). A.I.C.-D. is funded by an ERC consolidator
grant (ERC-2015-CoG–683163) and BIO2016-78955 grant from the Spanish Ministry of
Economy and Competitiveness. Exchanges between the Y.J. and T.B. laboratories were
funded by Tournesol grant 35656NB. B.K.M. was\r\nfunded by the Omics@vib Marie Curie
COFUND and Research Foundation Flanders for a postdoctoral fellowship."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: MM
full_name: Marquès-Bueno, MM
last_name: Marquès-Bueno
- first_name: L
full_name: Armengot, L
last_name: Armengot
- first_name: LC
full_name: Noack, LC
last_name: Noack
- first_name: J
full_name: Bareille, J
last_name: Bareille
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: MP
full_name: Platre, MP
last_name: Platre
- first_name: V
full_name: Bayle, V
last_name: Bayle
- first_name: M
full_name: Liu, M
last_name: Liu
- first_name: D
full_name: Opdenacker, D
last_name: Opdenacker
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
- first_name: BK
full_name: Möller, BK
last_name: Möller
- first_name: ZL
full_name: Nimchuk, ZL
last_name: Nimchuk
- first_name: T
full_name: Beeckman, T
last_name: Beeckman
- first_name: AI
full_name: Caño-Delgado, AI
last_name: Caño-Delgado
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Y
full_name: Jaillais, Y
last_name: Jaillais
citation:
ama: Marquès-Bueno M, Armengot L, Noack L, et al. Auxin-regulated reversible inhibition
of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism. Current
Biology. 2021;31(1). doi:10.1016/j.cub.2020.10.011
apa: Marquès-Bueno, M., Armengot, L., Noack, L., Bareille, J., Rodriguez Solovey,
L., Platre, M., … Jaillais, Y. (2021). Auxin-regulated reversible inhibition of
TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism. Current
Biology. Elsevier. https://doi.org/10.1016/j.cub.2020.10.011
chicago: Marquès-Bueno, MM, L Armengot, LC Noack, J Bareille, Lesia Rodriguez Solovey,
MP Platre, V Bayle, et al. “Auxin-Regulated Reversible Inhibition of TMK1 Signaling
by MAKR2 Modulates the Dynamics of Root Gravitropism.” Current Biology.
Elsevier, 2021. https://doi.org/10.1016/j.cub.2020.10.011.
ieee: M. Marquès-Bueno et al., “Auxin-regulated reversible inhibition of
TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism,” Current
Biology, vol. 31, no. 1. Elsevier, 2021.
ista: Marquès-Bueno M, Armengot L, Noack L, Bareille J, Rodriguez Solovey L, Platre
M, Bayle V, Liu M, Opdenacker D, Vanneste S, Möller B, Nimchuk Z, Beeckman T,
Caño-Delgado A, Friml J, Jaillais Y. 2021. Auxin-regulated reversible inhibition
of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism. Current
Biology. 31(1).
mla: Marquès-Bueno, MM, et al. “Auxin-Regulated Reversible Inhibition of TMK1 Signaling
by MAKR2 Modulates the Dynamics of Root Gravitropism.” Current Biology,
vol. 31, no. 1, Elsevier, 2021, doi:10.1016/j.cub.2020.10.011.
short: M. Marquès-Bueno, L. Armengot, L. Noack, J. Bareille, L. Rodriguez Solovey,
M. Platre, V. Bayle, M. Liu, D. Opdenacker, S. Vanneste, B. Möller, Z. Nimchuk,
T. Beeckman, A. Caño-Delgado, J. Friml, Y. Jaillais, Current Biology 31 (2021).
date_created: 2020-12-01T13:39:46Z
date_published: 2021-01-11T00:00:00Z
date_updated: 2024-10-21T06:02:09Z
day: '11'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1016/j.cub.2020.10.011
external_id:
isi:
- '000614361000039'
pmid:
- '33157019'
file:
- access_level: open_access
checksum: 30b3393d841fb2b1e2b22fb42b5c8fff
content_type: application/pdf
creator: dernst
date_created: 2021-02-04T11:37:50Z
date_updated: 2021-02-04T11:37:50Z
file_id: '9090'
file_name: 2021_CurrentBiology_MarquesBueno.pdf
file_size: 3458646
relation: main_file
success: 1
file_date_updated: 2021-02-04T11:37:50Z
has_accepted_license: '1'
intvolume: ' 31'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: Current Biology
publication_identifier:
eissn:
- 1879-0445
issn:
- 0960-9822
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin-regulated reversible inhibition of TMK1 signaling by MAKR2 modulates
the dynamics of root gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 31
year: '2021'
...
---
_id: '8992'
abstract:
- lang: eng
text: The phytohormone auxin plays a central role in shaping plant growth and development.
With decades of genetic and biochemical studies, numerous core molecular components
and their networks, underlying auxin biosynthesis, transport, and signaling, have
been identified. Notably, protein phosphorylation, catalyzed by kinases and oppositely
hydrolyzed by phosphatases, has been emerging to be a crucial type of post-translational
modification, regulating physiological and developmental auxin output at all levels.
In this review, we comprehensively discuss earlier and recent advances in our
understanding of genetics, biochemistry, and cell biology of the kinases and phosphatases
participating in auxin action. We provide insights into the mechanisms by which
reversible protein phosphorylation defines developmental auxin responses, discuss
current challenges, and provide our perspectives on future directions involving
the integration of the control of protein phosphorylation into the molecular auxin
network.
acknowledgement: This work was supported by the European Union’s Horizon 2020 Program
(ERC grant agreement no. 742985 to J.F.). S.T. was funded by a European Molecular
Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). C.L.
is supported by the Austrian Science Fund (FWF; P 31493).
article_processing_charge: No
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Tan S, Luschnig C, Friml J. Pho-view of auxin: Reversible protein phosphorylation
in auxin biosynthesis, transport and signaling. Molecular Plant. 2021;14(1):151-165.
doi:10.1016/j.molp.2020.11.004'
apa: 'Tan, S., Luschnig, C., & Friml, J. (2021). Pho-view of auxin: Reversible
protein phosphorylation in auxin biosynthesis, transport and signaling. Molecular
Plant. Elsevier. https://doi.org/10.1016/j.molp.2020.11.004'
chicago: 'Tan, Shutang, Christian Luschnig, and Jiří Friml. “Pho-View of Auxin:
Reversible Protein Phosphorylation in Auxin Biosynthesis, Transport and Signaling.”
Molecular Plant. Elsevier, 2021. https://doi.org/10.1016/j.molp.2020.11.004.'
ieee: 'S. Tan, C. Luschnig, and J. Friml, “Pho-view of auxin: Reversible protein
phosphorylation in auxin biosynthesis, transport and signaling,” Molecular
Plant, vol. 14, no. 1. Elsevier, pp. 151–165, 2021.'
ista: 'Tan S, Luschnig C, Friml J. 2021. Pho-view of auxin: Reversible protein phosphorylation
in auxin biosynthesis, transport and signaling. Molecular Plant. 14(1), 151–165.'
mla: 'Tan, Shutang, et al. “Pho-View of Auxin: Reversible Protein Phosphorylation
in Auxin Biosynthesis, Transport and Signaling.” Molecular Plant, vol.
14, no. 1, Elsevier, 2021, pp. 151–65, doi:10.1016/j.molp.2020.11.004.'
short: S. Tan, C. Luschnig, J. Friml, Molecular Plant 14 (2021) 151–165.
date_created: 2021-01-03T23:01:23Z
date_published: 2021-01-04T00:00:00Z
date_updated: 2025-07-10T12:01:28Z
day: '04'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.molp.2020.11.004
ec_funded: 1
external_id:
isi:
- '000605359400014'
pmid:
- '33186755'
file:
- access_level: open_access
checksum: 917e60e57092f22e16beac70b1775ea6
content_type: application/pdf
creator: dernst
date_created: 2021-01-07T14:03:53Z
date_updated: 2021-01-07T14:03:53Z
file_id: '8995'
file_name: 2020_MolecularPlant_Tan.pdf
file_size: 871088
relation: main_file
success: 1
file_date_updated: 2021-01-07T14:03:53Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 151-165
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
grant_number: 723-2015
name: Molecular Mechanism underlying Salicylic Acid Regulation of Endocytic Trafficking
in Arabidopsis
publication: Molecular Plant
publication_identifier:
eissn:
- 1752-9867
issn:
- 1674-2052
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Pho-view of auxin: Reversible protein phosphorylation in auxin biosynthesis,
transport and signaling'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2021'
...
---
_id: '8993'
abstract:
- lang: eng
text: N-1-naphthylphthalamic acid (NPA) is a key inhibitor of directional (polar)
transport of the hormone auxin in plants. For decades, it has been a pivotal tool
in elucidating the unique polar auxin transport-based processes underlying plant
growth and development. Its exact mode of action has long been sought after and
is still being debated, with prevailing mechanistic schemes describing only indirect
connections between NPA and the main transporters responsible for directional
transport, namely PIN auxin exporters. Here we present data supporting a model
in which NPA associates with PINs in a more direct manner than hitherto postulated.
We show that NPA inhibits PIN activity in a heterologous oocyte system and that
expression of NPA-sensitive PINs in plant, yeast, and oocyte membranes leads to
specific saturable NPA binding. We thus propose that PINs are a bona fide NPA
target. This offers a straightforward molecular basis for NPA inhibition of PIN-dependent
auxin transport and a logical parsimonious explanation for the known physiological
effects of NPA on plant growth, as well as an alternative hypothesis to interpret
past and future results. We also introduce PIN dimerization and describe an effect
of NPA on this, suggesting that NPA binding could be exploited to gain insights
into structural aspects of PINs related to their transport mechanism.
acknowledgement: "This work was supported by Austrian Science Fund Grant FWF P21533-B20
(to L.A.); German Research Foundation Grant DFG HA3468/6-1 (to U.Z.H.); and European
Research Council Grant 742985 (to J.F.). We thank Herta Steinkellner and Alexandra
Castilho for N. benthamiana plants, Fabian Nagelreiter for statistical advice, Lanassa
Bassukas for help with [ɣ32P]-\r\nATP assays, and Josef Penninger for providing
access to mass spectrometry instruments at the Vienna BioCenter Core Facilities.
We thank PNAS reviewers for the many comments and suggestions that helped to improve
this manuscript."
article_number: e2020857118
article_processing_charge: No
article_type: original
author:
- first_name: Lindy
full_name: Abas, Lindy
last_name: Abas
- first_name: Martina
full_name: Kolb, Martina
last_name: Kolb
- first_name: Johannes
full_name: Stadlmann, Johannes
last_name: Stadlmann
- first_name: Dorina P.
full_name: Janacek, Dorina P.
last_name: Janacek
- first_name: Kristina
full_name: Lukic, Kristina
id: 2B04DB84-F248-11E8-B48F-1D18A9856A87
last_name: Lukic
orcid: 0000-0003-1581-881X
- first_name: Claus
full_name: Schwechheimer, Claus
last_name: Schwechheimer
- first_name: Leonid A
full_name: Sazanov, Leonid A
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
- first_name: Lukas
full_name: Mach, Lukas
last_name: Mach
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ulrich Z.
full_name: Hammes, Ulrich Z.
last_name: Hammes
citation:
ama: Abas L, Kolb M, Stadlmann J, et al. Naphthylphthalamic acid associates with
and inhibits PIN auxin transporters. Proceedings of the National Academy of
Sciences of the United States of America. 2021;118(1). doi:10.1073/pnas.2020857118
apa: Abas, L., Kolb, M., Stadlmann, J., Janacek, D. P., Lukic, K., Schwechheimer,
C., … Hammes, U. Z. (2021). Naphthylphthalamic acid associates with and inhibits
PIN auxin transporters. Proceedings of the National Academy of Sciences of
the United States of America. National Academy of Sciences. https://doi.org/10.1073/pnas.2020857118
chicago: Abas, Lindy, Martina Kolb, Johannes Stadlmann, Dorina P. Janacek, Kristina
Lukic, Claus Schwechheimer, Leonid A Sazanov, Lukas Mach, Jiří Friml, and Ulrich
Z. Hammes. “Naphthylphthalamic Acid Associates with and Inhibits PIN Auxin Transporters.”
Proceedings of the National Academy of Sciences of the United States of America.
National Academy of Sciences, 2021. https://doi.org/10.1073/pnas.2020857118.
ieee: L. Abas et al., “Naphthylphthalamic acid associates with and inhibits
PIN auxin transporters,” Proceedings of the National Academy of Sciences of
the United States of America, vol. 118, no. 1. National Academy of Sciences,
2021.
ista: Abas L, Kolb M, Stadlmann J, Janacek DP, Lukic K, Schwechheimer C, Sazanov
LA, Mach L, Friml J, Hammes UZ. 2021. Naphthylphthalamic acid associates with
and inhibits PIN auxin transporters. Proceedings of the National Academy of Sciences
of the United States of America. 118(1), e2020857118.
mla: Abas, Lindy, et al. “Naphthylphthalamic Acid Associates with and Inhibits PIN
Auxin Transporters.” Proceedings of the National Academy of Sciences of the
United States of America, vol. 118, no. 1, e2020857118, National Academy of
Sciences, 2021, doi:10.1073/pnas.2020857118.
short: L. Abas, M. Kolb, J. Stadlmann, D.P. Janacek, K. Lukic, C. Schwechheimer,
L.A. Sazanov, L. Mach, J. Friml, U.Z. Hammes, Proceedings of the National Academy
of Sciences of the United States of America 118 (2021).
date_created: 2021-01-03T23:01:23Z
date_published: 2021-01-05T00:00:00Z
date_updated: 2026-06-18T19:38:20Z
day: '05'
ddc:
- '580'
department:
- _id: JiFr
- _id: LeSa
doi: 10.1073/pnas.2020857118
ec_funded: 1
external_id:
isi:
- '000607270100073'
pmid:
- '33443187'
intvolume: ' 118'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1073/pnas.2020857118
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1073/pnas.2102232118
scopus_import: '1'
status: public
title: Naphthylphthalamic acid associates with and inhibits PIN auxin transporters
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 118
year: '2021'
...
---
_id: '9189'
abstract:
- lang: eng
text: Transposable elements exist widely throughout plant genomes and play important
roles in plant evolution. Auxin is an important regulator that is traditionally
associated with root development and drought stress adaptation. The DEEPER ROOTING
1 (DRO1) gene is a key component of rice drought avoidance. Here, we identified
a transposon that acts as an autonomous auxin‐responsive promoter and its presence
at specific genome positions conveys physiological adaptations related to drought
avoidance. Rice varieties with high and auxin‐mediated transcription of DRO1 in
the root tip show deeper and longer root phenotypes and are thus better adapted
to drought. The INDITTO2 transposon contains an auxin response element and displays
auxin‐responsive promoter activity; it is thus able to convey auxin regulation
of transcription to genes in its proximity. In the rice Acuce, which displays
DRO1‐mediated drought adaptation, the INDITTO2 transposon was found to be inserted
at the promoter region of the DRO1 locus. Transgenesis‐based insertion of the
INDITTO2 transposon into the DRO1 promoter of the non‐adapted rice variety Nipponbare
was sufficient to promote its drought avoidance. Our data identify an example
of how transposons can act as promoters and convey hormonal regulation to nearby
loci, improving plant fitness in response to different abiotic stresses.
article_processing_charge: No
article_type: original
author:
- first_name: Y
full_name: Zhao, Y
last_name: Zhao
- first_name: L
full_name: Wu, L
last_name: Wu
- first_name: Q
full_name: Fu, Q
last_name: Fu
- first_name: D
full_name: Wang, D
last_name: Wang
- first_name: J
full_name: Li, J
last_name: Li
- first_name: B
full_name: Yao, B
last_name: Yao
- first_name: S
full_name: Yu, S
last_name: Yu
- first_name: L
full_name: Jiang, L
last_name: Jiang
- first_name: J
full_name: Qian, J
last_name: Qian
- first_name: X
full_name: Zhou, X
last_name: Zhou
- first_name: L
full_name: Han, L
last_name: Han
- first_name: S
full_name: Zhao, S
last_name: Zhao
- first_name: C
full_name: Ma, C
last_name: Ma
- first_name: Y
full_name: Zhang, Y
last_name: Zhang
- first_name: C
full_name: Luo, C
last_name: Luo
- first_name: Q
full_name: Dong, Q
last_name: Dong
- first_name: S
full_name: Li, S
last_name: Li
- first_name: L
full_name: Zhang, L
last_name: Zhang
- first_name: X
full_name: Jiang, X
last_name: Jiang
- first_name: Y
full_name: Li, Y
last_name: Li
- first_name: H
full_name: Luo, H
last_name: Luo
- first_name: K
full_name: Li, K
last_name: Li
- first_name: J
full_name: Yang, J
last_name: Yang
- first_name: Q
full_name: Luo, Q
last_name: Luo
- first_name: L
full_name: Li, L
last_name: Li
- first_name: S
full_name: Peng, S
last_name: Peng
- first_name: H
full_name: Huang, H
last_name: Huang
- first_name: Z
full_name: Zuo, Z
last_name: Zuo
- first_name: C
full_name: Liu, C
last_name: Liu
- first_name: L
full_name: Wang, L
last_name: Wang
- first_name: C
full_name: Li, C
last_name: Li
- first_name: X
full_name: He, X
last_name: He
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Y
full_name: Du, Y
last_name: Du
citation:
ama: Zhao Y, Wu L, Fu Q, et al. INDITTO2 transposon conveys auxin-mediated DRO1
transcription for rice drought avoidance. Plant, Cell & Environment.
2021;44(6):1846-1857. doi:10.1111/pce.14029
apa: Zhao, Y., Wu, L., Fu, Q., Wang, D., Li, J., Yao, B., … Du, Y. (2021). INDITTO2
transposon conveys auxin-mediated DRO1 transcription for rice drought avoidance.
Plant, Cell & Environment. Wiley. https://doi.org/10.1111/pce.14029
chicago: Zhao, Y, L Wu, Q Fu, D Wang, J Li, B Yao, S Yu, et al. “INDITTO2 Transposon
Conveys Auxin-Mediated DRO1 Transcription for Rice Drought Avoidance.” Plant,
Cell & Environment. Wiley, 2021. https://doi.org/10.1111/pce.14029.
ieee: Y. Zhao et al., “INDITTO2 transposon conveys auxin-mediated DRO1 transcription
for rice drought avoidance,” Plant, Cell & Environment, vol. 44, no.
6. Wiley, pp. 1846–1857, 2021.
ista: Zhao Y, Wu L, Fu Q, Wang D, Li J, Yao B, Yu S, Jiang L, Qian J, Zhou X, Han
L, Zhao S, Ma C, Zhang Y, Luo C, Dong Q, Li S, Zhang L, Jiang X, Li Y, Luo H,
Li K, Yang J, Luo Q, Li L, Peng S, Huang H, Zuo Z, Liu C, Wang L, Li C, He X,
Friml J, Du Y. 2021. INDITTO2 transposon conveys auxin-mediated DRO1 transcription
for rice drought avoidance. Plant, Cell & Environment. 44(6), 1846–1857.
mla: Zhao, Y., et al. “INDITTO2 Transposon Conveys Auxin-Mediated DRO1 Transcription
for Rice Drought Avoidance.” Plant, Cell & Environment, vol. 44, no.
6, Wiley, 2021, pp. 1846–57, doi:10.1111/pce.14029.
short: Y. Zhao, L. Wu, Q. Fu, D. Wang, J. Li, B. Yao, S. Yu, L. Jiang, J. Qian,
X. Zhou, L. Han, S. Zhao, C. Ma, Y. Zhang, C. Luo, Q. Dong, S. Li, L. Zhang, X.
Jiang, Y. Li, H. Luo, K. Li, J. Yang, Q. Luo, L. Li, S. Peng, H. Huang, Z. Zuo,
C. Liu, L. Wang, C. Li, X. He, J. Friml, Y. Du, Plant, Cell & Environment
44 (2021) 1846–1857.
date_created: 2021-02-24T10:07:21Z
date_published: 2021-06-01T00:00:00Z
date_updated: 2023-11-07T08:18:36Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/pce.14029
external_id:
isi:
- '000625398600001'
pmid:
- '33576018'
file:
- access_level: open_access
checksum: a812418fede076741c9c4dc07f317068
content_type: application/pdf
creator: amally
date_created: 2023-11-02T17:02:11Z
date_updated: 2023-11-02T17:02:11Z
file_id: '14481'
file_name: Zhao PlantCellEnv 2021_accepted.pdf
file_size: 8437528
relation: main_file
success: 1
file_date_updated: 2023-11-02T17:02:11Z
has_accepted_license: '1'
intvolume: ' 44'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Submitted Version
page: 1846-1857
pmid: 1
publication: Plant, Cell & Environment
publication_identifier:
eissn:
- 1365-3040
issn:
- 0140-7791
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: INDITTO2 transposon conveys auxin-mediated DRO1 transcription for rice drought
avoidance
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 44
year: '2021'
...