[{"date_updated":"2026-02-12T14:04:04Z","OA_place":"publisher","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Matteo","last_name":"Busato","full_name":"Busato, Matteo"},{"full_name":"Tuccillo, Mariarosaria","last_name":"Tuccillo","first_name":"Mariarosaria"},{"first_name":"Arcangelo","full_name":"Celeste, Arcangelo","last_name":"Celeste"},{"first_name":"Alessandro","full_name":"Tofoni, Alessandro","last_name":"Tofoni"},{"first_name":"Laura","full_name":"Silvestri, Laura","last_name":"Silvestri"},{"last_name":"D’Angelo","full_name":"D’Angelo, Paola","first_name":"Paola"},{"first_name":"Stefan Alexander","orcid":"0000-0003-2902-5319","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425","full_name":"Freunberger, Stefan Alexander","last_name":"Freunberger"},{"last_name":"Brutti","full_name":"Brutti, Sergio","first_name":"Sergio"}],"title":"Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation","citation":{"mla":"Busato, Matteo, et al. “Structural Rearrangements of a Cobalt-Free Lithium-Rich Layered Oxide Cathode during Formation.” ACS Applied Energy Materials, vol. 9, no. 1, American Chemical Society, 2026, pp. 686–97, doi:10.1021/acsaem.5c03511.","chicago":"Busato, Matteo, Mariarosaria Tuccillo, Arcangelo Celeste, Alessandro Tofoni, Laura Silvestri, Paola D’Angelo, Stefan Alexander Freunberger, and Sergio Brutti. “Structural Rearrangements of a Cobalt-Free Lithium-Rich Layered Oxide Cathode during Formation.” ACS Applied Energy Materials. American Chemical Society, 2026. https://doi.org/10.1021/acsaem.5c03511.","ista":"Busato M, Tuccillo M, Celeste A, Tofoni A, Silvestri L, D’Angelo P, Freunberger SA, Brutti S. 2026. Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation. ACS Applied Energy Materials. 9(1), 686–697.","short":"M. Busato, M. Tuccillo, A. Celeste, A. Tofoni, L. Silvestri, P. D’Angelo, S.A. Freunberger, S. Brutti, ACS Applied Energy Materials 9 (2026) 686–697.","apa":"Busato, M., Tuccillo, M., Celeste, A., Tofoni, A., Silvestri, L., D’Angelo, P., … Brutti, S. (2026). Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation. ACS Applied Energy Materials. American Chemical Society. https://doi.org/10.1021/acsaem.5c03511","ieee":"M. Busato et al., “Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation,” ACS Applied Energy Materials, vol. 9, no. 1. American Chemical Society, pp. 686–697, 2026.","ama":"Busato M, Tuccillo M, Celeste A, et al. Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation. ACS Applied Energy Materials. 2026;9(1):686-697. doi:10.1021/acsaem.5c03511"},"year":"2026","article_processing_charge":"Yes (via OA deal)","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["2574-0962"]},"abstract":[{"text":"Formation during the first cycles of Li-rich layered oxide (LRLO) cathode materials consolidates the interphase and leads to structural changes that are decisive for long-term cyclability. However, the nature and effect of the changes are material-dependent and unknown for the important class of Co-free, Ni-poor LRLOs. Here, we analyze the processes during the tailored formation procedure of a typical class member, Li1.28Ni0.15Mn0.57O2, and demonstrate that it remarkably changes lattice composition and structure as a prerequisite for stable cycling. We combine electrochemistry, operando mass spectrometry, X-ray diffraction, and X-ray absorption spectroscopy with density functional theory simulations. Activation most prominently compresses the layer spacing along the c-axis and increases reversible structural breathing. The large capacity of ∼250 mAh g–1 originates from the Ni2+/Ni4+ and O2–/O– redox couples. Electron exchange during O-redox is smeared over the entire anionic sublattice rather than localized on specific oxygen atomic sites. This redox mechanism is reversible without detrimental oxygen evolution, avoiding continued degradation common in conventional LRLOs. Sequential Ni- and O-redox during activation irreversibly distorts the coordination of the redox-inactive Mn4+ centers. This structural evolution of the MnO6 octahedra appears to enable the superior electrochemical performance of this LRLO phase. These findings define an activation pathway for the important class of Co-free, Ni-poor LRLOs, offering potential guidance for the rational design of high-performance, more sustainable cathode materials.","lang":"eng"}],"date_published":"2026-01-12T00:00:00Z","department":[{"_id":"StFr"}],"doi":"10.1021/acsaem.5c03511","PlanS_conform":"1","intvolume":" 9","OA_type":"hybrid","date_created":"2026-01-25T23:01:40Z","scopus_import":"1","quality_controlled":"1","file_date_updated":"2026-02-12T13:55:28Z","issue":"1","article_type":"original","ddc":["540"],"oa":1,"status":"public","page":"686-697","acknowledgement":"Elettra-Sincrotrone Trieste S.C.p.A. and its staff are acknowledged for providing synchrotron radiation beamtime and laboratory facilities, in particular the MCX and XAFS beamlines, where the XRD and XAS experiments have been carried out, supported by the projects number: 20217082, 20205109, and 20195014. This study was carried out within the MOST─Sustainable Mobility Center and received funding from the European Union Next-Generation EU (PIANO NAZIONALE DI RIPRESA E RESILIENZA (PNRR)─MISSIONE 4 COMPONENTE 2, INVESTIMENTO 1.4─D.D. 1033 17/06/2022, CN00000023). Moreover, the contribution of S.B. and A.C. to this study was carried out within the NEST─Network for Energy Sustainable Transition and received funding from the European Union Next-Generation EU (PNRR─MISSIONE 4 COMPONENTE 2, INVESTIMENTO 1.3─D.D. 1561 11/10/2022, B53C22004070006). This manuscript reflects only the authors’ views and opinions, neither the European Union nor the European Commission can be considered responsible for them. Two of us, S.B. and S.A.F., would like to thank the Alistore ERI. L.S. received funds from the Ministry of Ecological Transition in the “Ricerca di Sistema Elettrico” framework. S.A.F. is indebted to ISTA for support. The Scientific Service Units of ISTA supported this research through resources provided by the Lab Support Facility and the Miba Machine Shop.","type":"journal_article","publication_status":"published","corr_author":"1","file":[{"success":1,"file_id":"21222","date_created":"2026-02-12T13:55:28Z","checksum":"81272c19df41c696c1737168d3ea8c16","file_size":5977526,"file_name":"2026_AppliedEnergyMaterials_Busato.pdf","access_level":"open_access","relation":"main_file","creator":"dernst","content_type":"application/pdf","date_updated":"2026-02-12T13:55:28Z"}],"month":"01","volume":9,"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"_id":"21040","publication":"ACS Applied Energy Materials","day":"12","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"LifeSc"}],"publisher":"American Chemical Society","has_accepted_license":"1"},{"citation":{"mla":"Becker, Lea Marie, et al. Additional Data for “Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.” Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-21145.","short":"L.M. Becker, P. Schanda, C. Chipot, (2026).","chicago":"Becker, Lea Marie, Paul Schanda, and Christophe Chipot. “Additional Data for ‘Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.’” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21145.","ista":"Becker LM, Schanda P, Chipot C. 2026. Additional Data for ‘Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes’, Institute of Science and Technology Austria, 10.15479/AT-ISTA-21145.","apa":"Becker, L. M., Schanda, P., & Chipot, C. (2026). Additional Data for “Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.” Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21145","ieee":"L. M. Becker, P. Schanda, and C. Chipot, “Additional Data for ‘Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.’” Institute of Science and Technology Austria, 2026.","ama":"Becker LM, Schanda P, Chipot C. Additional Data for “Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.” 2026. doi:10.15479/AT-ISTA-21145"},"year":"2026","article_processing_charge":"No","contributor":[{"last_name":"Fu","contributor_type":"researcher","first_name":"Haohao"},{"first_name":"Benjamin","id":"71cda2f3-e604-11ee-a1df-da10587eda3f","contributor_type":"researcher","last_name":"Tatman"},{"last_name":"Dreydoppel","contributor_type":"researcher","first_name":"Matthias"},{"contributor_type":"researcher","last_name":"Kapitonova","first_name":"Anna","id":"9fb2a840-89e1-11ee-a8b7-cc5c7ba62471"},{"last_name":"Balazs","contributor_type":"researcher","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","orcid":"0000-0001-7597-043X","first_name":"Daniel"},{"last_name":"Weininger","contributor_type":"researcher","first_name":"Ulrich"},{"last_name":"Engilberge","contributor_type":"researcher","first_name":"Sylvain"}],"related_material":{"record":[{"status":"public","id":"20641","relation":"earlier_version"}]},"date_updated":"2026-02-18T10:04:44Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"id":"36336939-eb97-11eb-a6c2-c83f1214ca79","orcid":"0000-0002-6401-5151","first_name":"Lea Marie","last_name":"Becker","full_name":"Becker, Lea Marie"},{"first_name":"Paul","orcid":"0000-0002-9350-7606","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","full_name":"Schanda, Paul","last_name":"Schanda"},{"first_name":"Christophe","full_name":"Chipot, Christophe","last_name":"Chipot"}],"title":"Additional Data for \"Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes\"","doi":"10.15479/AT-ISTA-21145","date_created":"2026-02-05T13:54:39Z","file_date_updated":"2026-02-05T13:52:41Z","abstract":[{"text":"Protein conformational energy landscapes are shaped not only by intramolecular interactions but also by their environment. In protein crystals and protein-protein complexes, intermolecular contacts alter this energy landscape, but the exact nature of this alteration is difficult to decipher. Understanding how the crystal lattice affects protein dynamics is crucial for crystallography-based studies of motion, yet its influence on collective motions remains unclear. Aromatic ring flips in the hydrophobic core represent sensitive probes of such dynamics. Here, we compare the kinetics of aromatic ring flips in the protein GB1 in crystals, in complex with its binding partner IgG, and in solution, combining advanced isotope labeling with quantitative NMR methods. We show that rings in the core flip nearly a thousand times less frequently in crystals than in solution. Enhanced-sampling molecular dynamics simulations, based on a new crystal structure, reproduce these elevated barriers and reveal how the crystal restrains motions. ","lang":"eng"}],"date_published":"2026-02-09T00:00:00Z","department":[{"_id":"GradSch"},{"_id":"PaSc"}],"type":"research_data","file":[{"content_type":"text/plain","date_updated":"2026-02-05T13:52:37Z","creator":"lbecker","relation":"table_of_contents","access_level":"open_access","file_size":4263,"date_created":"2026-02-05T13:52:37Z","checksum":"02a419cce8cea450bc952f35488d2df5","file_name":"README.txt","file_id":"21146"},{"relation":"main_file","access_level":"open_access","date_updated":"2026-02-05T13:52:41Z","content_type":"application/zip","creator":"lbecker","file_id":"21147","success":1,"file_name":"Research_Data.zip","checksum":"b0b82b1aa73985b0b308a3fa52d21aea","date_created":"2026-02-05T13:52:41Z","file_size":50647107}],"month":"02","corr_author":"1","status":"public","ddc":["572"],"oa":1,"acknowledgement":"We thank Nikolai R. Skrynnikov and Olga O. Lebedenko (St. Petersburg) for insightful discussions and for performing exploratory MD simulations. We are grateful to Tobias Schubeis (Lyon) for advice with GB1 crystallization, and Rebecca Schmid for initial crystallization trials.\r\nWe thank Sebastian Falkner for assistance with constructing the structural model of the IgG:GB1 complex.\r\nThis research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance and the Lab Support Facilities. We thank Petra Rovó and Margarita Valhondo Falcón for excellent support of the NMR facility.\r\nLea M. Becker is recipient of a DOC fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology Austria (grant no. PR10660EAW01). Christophe Chipot acknowledges the European Research Council (grant project 101097272 ``MilliInMicro'') and the Métropole du Grand Nancy (grant project ``ARC''). BM07-FIP2 is supported by the French ANR PIA3 (France 2030) EquipEx+ project MAGNIFIX under grant agreement ANR-21-ESRE-0011.","acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"publisher":"Institute of Science and Technology Austria","project":[{"name":"Exploring protein dynamics by solid-state MAS NMR through specific labeling approaches","_id":"7be609c4-9f16-11ee-852c-85015ce2b9b0","grant_number":"26777"}],"has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png"},"oa_version":"Published Version","_id":"21145","day":"09"},{"OA_type":"free access","date_created":"2026-02-17T10:17:14Z","doi":"10.15479/AT-ISTA-21284","file_date_updated":"2026-02-17T10:11:14Z","date_published":"2026-02-18T00:00:00Z","abstract":[{"text":"The advantageous characteristics attributed to the 19F nucleus have made it a popular target for NMR once again in recent years. Aside from solution NMR, an increasing number of studies have been conducted applying solid-state magic-angle-spinning NMR to fluorine-labeled samples. Here, the high chemical shift anisotropy and strong dipolar couplings can be utilized to get structural insights into proteins and measure long distances. Despite increasing popularity and promising benefits, the sensitivity of biomolecular 19F MAS NMR often suffers from slow longitudinal T1 relaxation and therefore long recycle delays. In this work, we expand paramagnetic doping, an approach commonly used to reduce proton T1 relaxation times, to 19F-labeled biological samples. We study the effect of Gd(DTPA) and Gd(DTPA-BMA) on 19F and 13C T1 and T2 relaxation in a [5-19F13C]-tryptophan-labeled protein via 19F-detected MAS NMR experiments. The observed paramagnetic relaxation enhancement substantially reduces measurement times of 19F MAS NMR experiments without compromising resolution. Additionally, we report the chemical-shift assignments of all four fluorotryptophan signals in the 12 × 39 kDa large protein using a mutagenesis approach.","lang":"eng"}],"department":[{"_id":"GradSch"},{"_id":"PaSc"}],"year":"2026","citation":{"ama":"Becker LM, Schanda P. Research data for “Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants.” 2026. doi:10.15479/AT-ISTA-21284","ieee":"L. M. Becker and P. Schanda, “Research data for ‘Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants.’” Institute of Science and Technology Austria, 2026.","ista":"Becker LM, Schanda P. 2026. Research data for ‘Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants’, Institute of Science and Technology Austria, 10.15479/AT-ISTA-21284.","chicago":"Becker, Lea Marie, and Paul Schanda. “Research Data for ‘Accelerated 19F Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.’” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21284.","short":"L.M. Becker, P. Schanda, (2026).","mla":"Becker, Lea Marie, and Paul Schanda. Research Data for “Accelerated 19F Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.” Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-21284.","apa":"Becker, L. M., & Schanda, P. (2026). Research data for “Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants.” Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21284"},"article_processing_charge":"No","date_updated":"2026-02-18T10:12:49Z","contributor":[{"first_name":"Giorgia","id":"334a5e40-8747-11f0-b671-ba1f5154b4b4","contributor_type":"researcher","last_name":"Toscano"},{"contributor_type":"researcher","last_name":"Kapitonova","first_name":"Anna","id":"9fb2a840-89e1-11ee-a8b7-cc5c7ba62471"},{"last_name":"Singh","contributor_type":"researcher","id":"a3089acd-6806-11ee-bacc-f0c7d500ad20","first_name":"Rajkumar"},{"last_name":"Guillerm","contributor_type":"researcher","id":"bb74f472-ae54-11eb-9835-bc9c22fb1183","first_name":"Undina"},{"contributor_type":"researcher","last_name":"Lichtenecker","first_name":"Roman"}],"title":"Research data for \"Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants\"","user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","author":[{"orcid":"0000-0002-6401-5151","first_name":"Lea Marie","id":"36336939-eb97-11eb-a6c2-c83f1214ca79","full_name":"Becker, Lea Marie","last_name":"Becker"},{"last_name":"Schanda","full_name":"Schanda, Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","orcid":"0000-0002-9350-7606","first_name":"Paul"}],"OA_place":"repository","publisher":"Institute of Science and Technology Austria","acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"has_accepted_license":"1","oa_version":"None","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png"},"day":"18","_id":"21284","type":"research_data","corr_author":"1","file":[{"checksum":"2d3105f26be578073b88ee1f2ea0bdb1","date_created":"2026-02-17T10:11:14Z","file_size":36996027,"file_name":"Research_data.zip","success":1,"file_id":"21285","creator":"lbecker","content_type":"application/zip","date_updated":"2026-02-17T10:11:14Z","access_level":"open_access","relation":"main_file"},{"access_level":"open_access","relation":"table_of_contents","creator":"lbecker","content_type":"text/plain","date_updated":"2026-02-17T10:11:14Z","file_id":"21286","checksum":"e24aebcdb8856cb181cbaa02de020ddb","file_size":1993,"date_created":"2026-02-17T10:11:14Z","file_name":"README.txt"}],"month":"2","status":"public","ddc":["541"],"oa":1,"acknowledgement":"We thank Ben P. Tatman for insightful discussions. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance Facility and the Lab Support Facility."},{"publication_identifier":{"issn":["2663-337X"]},"language":[{"iso":"eng"}],"article_processing_charge":"No","year":"2026","supervisor":[{"first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková"}],"citation":{"ama":"Riegler S. Root system plasticity under nutrient limitation : Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea species. 2026. doi:10.15479/AT-ISTA-21360","ieee":"S. Riegler, “Root system plasticity under nutrient limitation : Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea species,” Institute of Science and Technology Austria, 2026.","apa":"Riegler, S. (2026). Root system plasticity under nutrient limitation : Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea species. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21360","ista":"Riegler S. 2026. Root system plasticity under nutrient limitation : Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea species. Institute of Science and Technology Austria.","short":"S. Riegler, Root System Plasticity under Nutrient Limitation : Investigating Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea Species, Institute of Science and Technology Austria, 2026.","chicago":"Riegler, Stefan. “Root System Plasticity under Nutrient Limitation : Investigating Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea Species.” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21360.","mla":"Riegler, Stefan. Root System Plasticity under Nutrient Limitation : Investigating Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea Species. Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-21360."},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","title":"Root system plasticity under nutrient limitation : Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea species","author":[{"full_name":"Riegler, Stefan","last_name":"Riegler","orcid":"0000-0003-3413-1343","first_name":"Stefan","id":"FF6018E0-D806-11E9-8E43-0B14E6697425"}],"OA_place":"repository","date_updated":"2026-03-09T12:20:56Z","degree_awarded":"PhD","related_material":{"record":[{"status":"public","relation":"research_data","id":"21363"}]},"file_date_updated":"2026-03-02T10:59:50Z","date_created":"2026-02-27T09:08:14Z","doi":"10.15479/AT-ISTA-21360","alternative_title":["ISTA Thesis"],"department":[{"_id":"GradSch"},{"_id":"EvBe"}],"license":"https://creativecommons.org/licenses/by-sa/4.0/","date_published":"2026-02-26T00:00:00Z","month":"02","file":[{"access_level":"closed","relation":"source_file","creator":"sriegler","content_type":"application/x-zip-compressed","date_updated":"2026-03-02T10:59:50Z","file_id":"21386","file_size":31430022,"checksum":"2f1f44e8536c2538f94a440217452c9f","date_created":"2026-03-02T10:59:50Z","file_name":"2026_Riegler_Stefan_Thesis.zip"},{"access_level":"closed","embargo_to":"open_access","relation":"main_file","creator":"sriegler","date_updated":"2026-03-02T10:59:49Z","embargo":"2027-02-27","content_type":"application/pdf","file_id":"21387","file_name":"2026_Riegler_Stefan_Thesis.pdf","checksum":"2e8dc39640bc26ae5684c944c619719b","date_created":"2026-03-02T10:59:49Z","file_size":11635090}],"corr_author":"1","type":"dissertation","publication_status":"published","acknowledgement":"I would like to acknowledge the Austrian Academy of Sciences (ÖAW) and European\r\nResearch Executive Agency (REA) for funding my research (DOC ÖAW Fellowship\r\n26130, Horizon Europe BOLERO Project 101060393). ","page":"185","status":"public","ddc":["570","575","583"],"has_accepted_license":"1","publisher":"Institute of Science and Technology Austria","project":[{"name":"Breeding for coffee and cocoa root resilience in low input farming systems based on improved rootstocks","grant_number":"101060393","_id":"34afa094-11ca-11ed-8bc3-a375845a59fb"}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"day":"26","_id":"21360","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","image":"/images/cc_by_sa.png","short":"CC BY-SA (4.0)"},"oa_version":"Published Version"},{"type":"journal_article","oaworkid":1,"publication_status":"published","file":[{"file_id":"21026","success":1,"file_size":7335694,"date_created":"2026-01-21T08:21:11Z","checksum":"0ab7ac2fbcb61a364dba57152db64ed7","file_name":"2026_NaturePhysics_Mishra.pdf","relation":"main_file","access_level":"open_access","content_type":"application/pdf","date_updated":"2026-01-21T08:21:11Z","creator":"dernst"}],"month":"01","corr_author":"1","volume":22,"oa":1,"status":"public","ddc":["570"],"page":"139-150","acknowledgement":"We thank N. Petridou (EMBL) for sharing results before publication. N.M. was supported by funding from the European Union’s Horizon 2020 programme under the Marie Skłodowska-Curie COFUND Actions ISTplus grant agreement number 754411. Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement number 101034413. The research was supported by funding to C.-P.H. from the NOMIS Foundation, Project ID 1.844. We would like to thank past and present members of the Heisenberg and Hannezo groups for discussions, particularly S. Shamipour, V. Doddihal, M. Jovic, N. Hino, F. N. Arslan, R. Kobylinska and C. Camelo for feedback on the draft manuscript. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria through resources provided by the Aquatics Facility, Imaging & Optics Facility (IOF), Scientific Computing (SciComp) facility and Lab Support Facility (LSF). Open access funding provided by Institute of Science and Technology (IST Austria).","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"publisher":"Springer Nature","project":[{"name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","call_identifier":"H2020","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"grant_number":"101034413","call_identifier":"H2020","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","name":"IST-BRIDGE: International postdoctoral program"},{"name":"Cytoplasmic self-organization into cell-like compartments as a common guiding principle in early animal development","_id":"917c023a-16d5-11f0-9cad-eb5cafc52090"}],"external_id":{"oaworkid":["W7118187193"]},"has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","_id":"21015","ec_funded":1,"day":"05","publication":"Nature Physics","citation":{"chicago":"Mishra, Nikhil, Yuting I Li, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” Nature Physics. Springer Nature, 2026. https://doi.org/10.1038/s41567-025-03122-1.","short":"N. Mishra, Y.I. Li, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 22 (2026) 139–150.","ista":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. 2026. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. Nature Physics. 22, 139–150.","mla":"Mishra, Nikhil, et al. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” Nature Physics, vol. 22, Springer Nature, 2026, pp. 139–50, doi:10.1038/s41567-025-03122-1.","apa":"Mishra, N., Li, Y. I., Hannezo, E. B., & Heisenberg, C.-P. J. (2026). Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-025-03122-1","ama":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. Nature Physics. 2026;22:139-150. doi:10.1038/s41567-025-03122-1","ieee":"N. Mishra, Y. I. Li, E. B. Hannezo, and C.-P. J. Heisenberg, “Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo,” Nature Physics, vol. 22. Springer Nature, pp. 139–150, 2026."},"year":"2026","article_processing_charge":"Yes (via OA deal)","publication_identifier":{"issn":["1745-2473"],"issnl":[" 1745-2473"],"eissn":["1745-2481"]},"language":[{"iso":"eng"}],"date_updated":"2026-03-16T13:18:36Z","OA_place":"publisher","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo","author":[{"id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E","orcid":"0000-0002-6425-5788","first_name":"Nikhil","last_name":"Mishra","full_name":"Mishra, Nikhil"},{"full_name":"Li, Yuting I","last_name":"Li","first_name":"Yuting I","id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","full_name":"Hannezo, Edouard B"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"PlanS_conform":"1","doi":"10.1038/s41567-025-03122-1","date_created":"2026-01-20T10:12:19Z","OA_type":"hybrid","intvolume":" 22","scopus_import":"1","quality_controlled":"1","article_type":"original","file_date_updated":"2026-01-21T08:21:11Z","abstract":[{"text":"Early embryo geometry is one of the most invariant species-specific traits, yet its role in ensuring developmental reproducibility and robustness remains underexplored. Here we show that in zebrafish, the geometry of the fertilized egg—specifically its curvature and volume—serves as a critical initial condition triggering a cascade of events that influence development. The embryo geometry guides patterned asymmetric cell divisions in the blastoderm, generating radial gradients of cell volume and nucleocytoplasmic ratio. These gradients generate mitotic phase waves, with the nucleocytoplasmic ratio determining individual cell cycle periods independently of other cells. We demonstrate that reducing cell autonomy reshapes these waves, emphasizing the instructive role of geometry-derived volume patterns in setting the intrinsic period of the cell cycle oscillator. In addition to organizing cell cycles, early embryo geometry spatially patterns zygotic genome activation at the midblastula transition, a key step in establishing embryonic autonomy. Disrupting the embryo shape alters the zygotic genome activation pattern and causes ectopic germ layer specification, underscoring the developmental significance of geometry. Together, our findings reveal a symmetry-breaking function of early embryo geometry in coordinating cell cycle and transcriptional patterning.","lang":"eng"}],"date_published":"2026-01-05T00:00:00Z","department":[{"_id":"EdHa"},{"_id":"CaHe"}]},{"article_processing_charge":"Yes (via OA deal)","language":[{"iso":"eng"}],"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"citation":{"ieee":"G. M. Grosjean et al., “Adventitious carbon breaks symmetry in oxide contact electrification,” Nature, vol. 651, no. 8106. Springer Nature, pp. 626–631, 2026.","ama":"Grosjean GM, Ostermann M, Sauer M, et al. Adventitious carbon breaks symmetry in oxide contact electrification. Nature. 2026;651(8106):626-631. doi:10.1038/s41586-025-10088-w","apa":"Grosjean, G. M., Ostermann, M., Sauer, M., Hahn, M., Pichler, C. M., Fahrnberger, F., … Waitukaitis, S. R. (2026). Adventitious carbon breaks symmetry in oxide contact electrification. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-10088-w","mla":"Grosjean, Galien M., et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” Nature, vol. 651, no. 8106, Springer Nature, 2026, pp. 626–31, doi:10.1038/s41586-025-10088-w.","chicago":"Grosjean, Galien M, Markus Ostermann, Markus Sauer, Michael Hahn, Christian M. Pichler, Florian Fahrnberger, Felix Pertl, et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” Nature. Springer Nature, 2026. https://doi.org/10.1038/s41586-025-10088-w.","ista":"Grosjean GM, Ostermann M, Sauer M, Hahn M, Pichler CM, Fahrnberger F, Pertl F, Balazs D, Link MM, Kim SH, Schrader DL, Blanco A, Gracia F, Mujica N, Waitukaitis SR. 2026. Adventitious carbon breaks symmetry in oxide contact electrification. Nature. 651(8106), 626–631.","short":"G.M. Grosjean, M. Ostermann, M. Sauer, M. Hahn, C.M. Pichler, F. Fahrnberger, F. Pertl, D. Balazs, M.M. Link, S.H. Kim, D.L. Schrader, A. Blanco, F. Gracia, N. Mujica, S.R. Waitukaitis, Nature 651 (2026) 626–631."},"year":"2026","OA_place":"publisher","title":"Adventitious carbon breaks symmetry in oxide contact electrification","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Grosjean","full_name":"Grosjean, Galien M","id":"0C5FDA4A-9CF6-11E9-8939-FF05E6697425","first_name":"Galien M","orcid":"0000-0001-5154-417X"},{"last_name":"Ostermann","full_name":"Ostermann, Markus","first_name":"Markus"},{"full_name":"Sauer, Markus","last_name":"Sauer","first_name":"Markus"},{"first_name":"Michael","last_name":"Hahn","full_name":"Hahn, Michael"},{"first_name":"Christian M.","full_name":"Pichler, Christian M.","last_name":"Pichler"},{"first_name":"Florian","full_name":"Fahrnberger, Florian","last_name":"Fahrnberger"},{"full_name":"Pertl, Felix","last_name":"Pertl","first_name":"Felix","orcid":"0000-0003-0463-5794","id":"6313aec0-15b2-11ec-abd3-ed67d16139af"},{"id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","first_name":"Daniel","orcid":"0000-0001-7597-043X","last_name":"Balazs","full_name":"Balazs, Daniel"},{"first_name":"Mason M.","full_name":"Link, Mason M.","last_name":"Link"},{"first_name":"Seong H.","full_name":"Kim, Seong H.","last_name":"Kim"},{"last_name":"Schrader","full_name":"Schrader, Devin L.","first_name":"Devin L."},{"first_name":"Adriana","full_name":"Blanco, Adriana","last_name":"Blanco"},{"last_name":"Gracia","full_name":"Gracia, Francisco","first_name":"Francisco"},{"full_name":"Mujica, Nicolás","last_name":"Mujica","first_name":"Nicolás"},{"full_name":"Waitukaitis, Scott R","last_name":"Waitukaitis","first_name":"Scott R","orcid":"0000-0002-2299-3176","id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87"}],"date_updated":"2026-03-24T06:59:57Z","quality_controlled":"1","file_date_updated":"2026-03-24T06:57:08Z","issue":"8106","article_type":"original","doi":"10.1038/s41586-025-10088-w","PlanS_conform":"1","intvolume":" 651","OA_type":"hybrid","date_created":"2026-03-23T15:04:00Z","department":[{"_id":"ScWa"},{"_id":"GradSch"},{"_id":"LifeSc"}],"abstract":[{"text":"Insulating oxides are among the most abundant solid materials in the universe1,2,3. Of the many ways in which they influence natural phenomena, perhaps the most consequential is their capacity to transfer electrical charge during contact4,5,6,7,8,9,10—which occurs even between samples of the same oxide—yet the symmetry-breaking parameter that causes this remains unidentified11,12. Here we show that adventitious carbonaceous molecules adsorbed from the environment are the symmetry-breaking factor in same-material oxide contact electrification (CE). We use acoustic levitation to measure charge exchange between a sphere and a plate composed of identical amorphous silicon dioxide (SiO2). Although charging polarity is random for co-prepared samples, we control it with baking or plasma treatment. Observing the charge-exchange relaxation afterwards, we see dynamics over a timescale of hours and connect this directly to the presence of adventitious carbon with time-of-flight mass spectrometry, low-energy ion scattering and infrared spectroscopy. Going further, we confirm that adventitious carbon can even determine charge exchange among different oxides. Our results identify the symmetry-breaking parameter that causes insulating oxides to exchange charge in settings ranging from desert sands4 to volcanic plumes5,6, while simultaneously highlighting an overlooked factor in CE more broadly.","lang":"eng"}],"date_published":"2026-03-18T00:00:00Z","corr_author":"1","file":[{"date_updated":"2026-03-24T06:57:08Z","content_type":"application/pdf","creator":"dernst","relation":"main_file","access_level":"open_access","file_name":"2026_Nature_Grosjean.pdf","date_created":"2026-03-24T06:57:08Z","file_size":12245694,"checksum":"dafef9ed575b44be4263e948a47ae056","file_id":"21494","success":1}],"month":"03","volume":651,"type":"journal_article","publication_status":"published","page":"626-631","acknowledgement":"This project has received support from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 949120) and from the Marie Skłodowska-Curie programme (grant agreement no. 754411). We acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. N.M. acknowledges support from grant Fondecyt 1221597. G.G. is a Serra Húnter fellow. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility and Lab Support Facility. We thank the Modic group for the use of the Laue camera, T. Zauner for the photography of the experimental set-up and R. Möller for insightful discussions. Open access funding provided by Institute of Science and Technology (IST Austria).","status":"public","oa":1,"ddc":["540"],"has_accepted_license":"1","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"external_id":{"pmid":["41851325"]},"project":[{"_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","grant_number":"949120","call_identifier":"H2020","name":"Tribocharge: a multi-scale approach to an enduring problem in physics"},{"name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"754411"}],"publisher":"Springer Nature","_id":"21485","ec_funded":1,"publication":"Nature","day":"18","pmid":1,"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"}},{"pmid":1,"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"_id":"21490","day":"23","publication":"Current Biology","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"Bio"},{"_id":"LifeSc"}],"project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"grant_number":"E271","_id":"bd906599-d553-11ed-ba76-abf8547645d7","name":"Identification of a novel regulator in auxin canalization"}],"external_id":{"pmid":["41831441"]},"publisher":"Elsevier","has_accepted_license":"1","status":"public","oa":1,"ddc":["580"],"page":"1468-1480.e6","acknowledgement":"We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.","type":"journal_article","publication_status":"published","corr_author":"1","month":"03","file":[{"creator":"dernst","date_updated":"2026-03-24T08:34:37Z","content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_name":"2026_CurrentBiology_Li.pdf","date_created":"2026-03-24T08:34:37Z","file_size":12986894,"checksum":"fe6c41fdab58a55df5f2a5860c02acdc","success":1,"file_id":"21496"}],"volume":36,"abstract":[{"text":"Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development.","lang":"eng"}],"date_published":"2026-03-23T00:00:00Z","department":[{"_id":"JiFr"}],"doi":"10.1016/j.cub.2026.02.023","PlanS_conform":"1","intvolume":" 36","OA_type":"hybrid","date_created":"2026-03-23T15:11:16Z","quality_controlled":"1","file_date_updated":"2026-03-24T08:34:37Z","article_type":"original","issue":"6","date_updated":"2026-03-24T08:36:40Z","OA_place":"publisher","author":[{"last_name":"Li","full_name":"Li, Mingyue","id":"01f96916-0235-11eb-9379-a323192643b7","first_name":"Mingyue"},{"first_name":"Nikola","last_name":"Rydza","full_name":"Rydza, Nikola"},{"first_name":"Ewa","full_name":"Mazur, Ewa","last_name":"Mazur"},{"last_name":"Molnar","full_name":"Molnar, Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Gergely"},{"last_name":"Nodzyński","full_name":"Nodzyński, Tomasz","first_name":"Tomasz"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří"}],"title":"Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"apa":"Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., & Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2026.02.023","chicago":"Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology. Elsevier, 2026. https://doi.org/10.1016/j.cub.2026.02.023.","ista":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6.","short":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6.","mla":"Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology, vol. 36, no. 6, Elsevier, 2026, p. 1468–1480.e6, doi:10.1016/j.cub.2026.02.023.","ama":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 2026;36(6):1468-1480.e6. doi:10.1016/j.cub.2026.02.023","ieee":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” Current Biology, vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026."},"year":"2026","article_processing_charge":"Yes (via OA deal)","language":[{"iso":"eng"}],"publication_identifier":{"issn":["0960-9822"]}},{"acknowledgement":"We thank all members of the Heisenberg, Henkes, and Hannezo groups for their support. We are also grateful to the Imaging and Optics, Scientific Computing, Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their technical assistance and support. Numerical simulations were performed using the computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020 research and innovation programme (grant agreement No. 665385). 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provided via this external link."}]},"date_updated":"2026-01-05T14:11:56Z","article_processing_charge":"No","citation":{"mla":"Danzl, Johann G., et al. Light-Microscopy Based Connectomic Reconstruction of Mammalian Brain Tissue. Institute of Science and Technology Austria, 2025, doi:10.15479/AT:ISTA:18697.","short":"J.G. Danzl, J. Lyudchik, C. Kreuzinger, (2025).","ista":"Danzl JG, Lyudchik J, Kreuzinger C. 2025. Light-microscopy based connectomic reconstruction of mammalian brain tissue, Institute of Science and Technology Austria, 10.15479/AT:ISTA:18697.","chicago":"Danzl, Johann G, Julia Lyudchik, and Caroline Kreuzinger. “Light-Microscopy Based Connectomic Reconstruction of Mammalian Brain Tissue.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT:ISTA:18697.","apa":"Danzl, J. G., Lyudchik, J., & Kreuzinger, C. (2025). Light-microscopy based connectomic reconstruction of mammalian brain tissue. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:18697","ieee":"J. G. Danzl, J. Lyudchik, and C. 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Here we developed light-microscopy based connectomics (LICONN). We integrated specifically engineered hydrogel embedding and expansion with comprehensive deep-learning based segmentation and analysis of connectivity, thus directly incorporating molecular information in synapse-level brain tissue reconstructions. LICONN will allow synapse-level brain tissue phenotyping in biological experiments in a readily adoptable manner."}],"date_published":"2025-03-03T00:00:00Z","file_date_updated":"2025-02-28T16:50:39Z","doi":"10.15479/AT:ISTA:18697","OA_type":"gold","date_created":"2024-12-20T09:22:20Z"},{"citation":{"apa":"Nikolic, N., Pleska, M., Bergmiller, T., & Guet, C. C. (2025). A bacterial toxin-antitoxin system as a native defence element against RNA phages. Biology Letters. The Royal Society. https://doi.org/10.1098/rsbl.2025.0080","ista":"Nikolic N, Pleska M, Bergmiller T, Guet CC. 2025. A bacterial toxin-antitoxin system as a native defence element against RNA phages. 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Most known bacterial antiphage defence systems target phages with DNA genomes, which raises the question of how bacteria defend against phages with RNA genomes. Bacterial toxin–antitoxin systems that cleave intracellular RNA could potentially protect bacteria against RNA phages, but this has not been explored experimentally. In this study, we investigated the role of a model toxin–antitoxin system, MazEF, in protecting Escherichia coli against two RNA phage species. When challenged with these phages, the native presence of mazEF moderately reduced population susceptibility and increased the survival of individual E. coli cells. Genomic analysis further revealed an underrepresentation of the MazF cleavage site in genomes of RNA phages infecting E. coli, indicating selection against cleavage. These results show that, in addition to other physiological roles, RNA-degrading toxin–antitoxin systems may also help defend against RNA phages."}],"date_published":"2025-06-11T00:00:00Z","department":[{"_id":"CaGu"}],"publication_status":"published","type":"journal_article","corr_author":"1","month":"06","file":[{"relation":"main_file","access_level":"open_access","content_type":"application/pdf","date_updated":"2025-06-23T11:34:39Z","creator":"dernst","file_id":"19873","success":1,"date_created":"2025-06-23T11:34:39Z","file_size":1850797,"checksum":"016f644ed068f8609ded306ad26dbd3f","file_name":"2025_BiologyLetters_Nikolic.pdf"}],"volume":21,"ddc":["570"],"oa":1,"status":"public","acknowledgement":"This work was supported by ISTFELLOW (People Program – Marie Curie Actions of the European Union’s Seventh Framework Program FP7 under REA grant agreement 291734), the FWF (Austrian Science Fund) Elise Richter Program project number V 738 and the Wellcome Trust Institutional Strategic Support Award (WT105618MA), to N.N. M.P. was a Simons Foundation Fellow of the Life Sciences Research Foundation. We are grateful to Kathrin Tomasek, Lisa Butt, Chris Estell, Alys Jepson, Franklin Nobrega, Stefano Pagliara, Remy Chait, Steve West, Vicki Gold, Josh Eaton, Ivana Gudelj and Rob Beardmore for useful discussions and technical support, as well as to Robin Wright, Christian Fitch and Ben Temperton for sharing equipment. We thank Laurence Van Melderen for sharing the strains. We acknowledge the IST Austria Lab Support Facility, LSI Technical Services Team at the University of Exeter and the Translational Research Exchange @ Exeter (TREE) network. N.N. is grateful to Fabrice Gielen for his support.","acknowledged_ssus":[{"_id":"LifeSc"}],"external_id":{"isi":["001505019800001"],"pmid":["40494395"]},"project":[{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"name":"Bacterial toxin-antitoxin systems as antiphage defense mechanisms","call_identifier":"FWF","grant_number":"V00738","_id":"26956E74-B435-11E9-9278-68D0E5697425"}],"publisher":"The Royal Society","has_accepted_license":"1","pmid":1,"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"ec_funded":1,"_id":"19857","day":"11","publication":"Biology Letters","article_number":"20250080"},{"user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","author":[{"last_name":"Schanda","full_name":"Schanda, Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","first_name":"Paul","orcid":"0000-0002-9350-7606"}],"title":"Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling Scheme","status":"public","contributor":[{"first_name":"Darja","last_name":"Rohden","contributor_type":"researcher"},{"last_name":"Napoli","contributor_type":"researcher","first_name":"Federico"},{"last_name":"Tatman","contributor_type":"researcher","first_name":"Ben"},{"contributor_type":"researcher","last_name":"Schanda","first_name":"Paul"}],"oa":1,"ddc":["572"],"related_material":{"record":[{"relation":"used_in_publication","id":"20258","status":"public"}]},"date_updated":"2025-12-29T14:52:16Z","file":[{"file_id":"19960","success":1,"date_created":"2025-07-03T10:30:14Z","file_size":1160,"checksum":"a2ef61aa9fb5313c7d426913eb0482c0","file_name":"README","relation":"main_file","access_level":"open_access","content_type":"application/octet-stream","date_updated":"2025-07-03T10:30:14Z","creator":"pschanda"},{"file_id":"19961","success":1,"file_name":"data_Arg_MASNMR_Rohden.zip","date_created":"2025-07-03T10:30:55Z","file_size":128597184,"checksum":"8fb77b96d0fcc95c9903005652207a8c","relation":"main_file","access_level":"open_access","date_updated":"2025-07-03T10:30:55Z","content_type":"application/zip","creator":"pschanda"},{"content_type":"application/x-xz","date_updated":"2025-08-14T07:06:58Z","creator":"pschanda","relation":"main_file","access_level":"open_access","file_size":4766564,"date_created":"2025-08-14T07:06:58Z","checksum":"a60cc16d20b089c4bef94040a99cfba5","file_name":"20240903_ubi_DN_Argd1C13_2D_spectra.tar.xz","file_id":"20172","success":1}],"month":"07","article_processing_charge":"No","corr_author":"1","citation":{"ama":"Schanda P. Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling Scheme. 2025. doi:10.15479/AT-ISTA-19956","ieee":"P. Schanda, “Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling Scheme.” Institute of Science and Technology Austria, 2025.","short":"P. Schanda, (2025).","chicago":"Schanda, Paul. “Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling Scheme.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19956.","ista":"Schanda P. 2025. Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling Scheme, Institute of Science and Technology Austria, 10.15479/AT-ISTA-19956.","mla":"Schanda, Paul. Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling Scheme. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19956.","apa":"Schanda, P. (2025). Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling Scheme. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19956"},"type":"research_data","year":"2025","_id":"19956","department":[{"_id":"PaSc"}],"day":"03","abstract":[{"text":"The specific introduction of 1H-13C or 1H-15N moieties into otherwise deuterated proteins holds great potential for high-resolution solution and magic-angle spinning (MAS) NMR studies of protein structure and dynamics. Arginine residues play key roles for example at active sites of enzymes. Taking advantage of a chemically synthesized Arg with a 13C-1H2 group in an otherwise deuterated backbone, we demonstrate here the usefulness of proton-detected arginine MAS NMR approaches to probe arginine dynamics. In experiments on crystalline ubiquitin and the 134 kDa tetrameric enzyme malate dehydrogenase we detected a wide range of motions, from sites that are rigid on time scales of at least tens of milliseconds to residues undergoing predominantly nanosecond motions. Spin-relaxation and dipolar-coupling measurements enabled quantitative determination of these dynamics. We observed microsecond dynamics of residue Arg54 in crystalline ubiquitin, whose backbone is known to sample different β-turn conformations on this time scale. The labeling scheme and experiments presented here expand the toolkit for high-resolution proton-detected MAS NMR","lang":"eng"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png"},"oa_version":"None","date_published":"2025-07-03T00:00:00Z","has_accepted_license":"1","file_date_updated":"2025-08-14T07:06:58Z","doi":"10.15479/AT-ISTA-19956","acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"publisher":"Institute of Science and Technology Austria","date_created":"2025-07-03T04:21:37Z","project":[{"_id":"eb9c82eb-77a9-11ec-83b8-aadd536561cf","grant_number":"I05812","name":"AlloSpace. The emergence and mechanisms of allostery"}]},{"publisher":"Embo Press","external_id":{"isi":["001511452100001"],"pmid":["40537284"]},"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","pmid":1,"publication":"Life Science Alliance","day":"01","article_number":"e202503208","_id":"19963","publication_status":"published","type":"journal_article","volume":8,"month":"09","file":[{"content_type":"application/pdf","date_updated":"2025-12-30T09:17:09Z","creator":"dernst","relation":"main_file","access_level":"open_access","file_size":5471288,"checksum":"591d47aa39fc969986c7d3b966890f5f","date_created":"2025-12-30T09:17:09Z","file_name":"2025_LifeScienceAlliance_Kuhn.pdf","file_id":"20904","success":1}],"corr_author":"1","oa":1,"ddc":["570"],"status":"public","acknowledgement":"All proteomics analysis was done by the ISTA LSF Mass Spectrometry Service: Ewelina Dutkiewicz-Kopczynska processed the samples (digest and cleanup); Bella Bruszel optimized the acquisition methods, acquired the data, and performed all searches; and Armel Nicolas provided pre- and post-project consulting and post-processed the search results using a development version of their data analysis package, proteoCraft (publication pending). The authors would like to thank Saki for their clarity of thought and insight, as well as Dr. Lorenzo Puri and the members of his laboratory for invaluable discussions relating to the project. This research was further supported by the Lab Support Facility and the Imaging and Optics Facility of ISTA.","OA_type":"gold","date_created":"2025-07-06T22:01:22Z","intvolume":" 8","PlanS_conform":"1","doi":"10.26508/lsa.202503208","article_type":"original","issue":"9","file_date_updated":"2025-12-30T09:17:09Z","scopus_import":"1","quality_controlled":"1","date_published":"2025-09-01T00:00:00Z","abstract":[{"lang":"eng","text":"The acquisition of cellular identity requires large-scale alterations in cellular state. The noncanonical proteasome activator PSME3 is known to regulate diverse cellular processes, but its importance for differentiation remains unclear. Here, we demonstrate that PSME3 binds dynamically to highly active promoters over the course of differentiation. However, loss of PSME3 does not globally affect mRNA transcription. We find instead that PSME3 influences the levels of several adhesion-related proteins and acts upstream of the HSP90 co-chaperone NUDC to regulate cell motility and myoblast differentiation in a proteasome-independent manner. Our findings reveal several new facets of PSME3 functionality and highlight its importance for the differentiation of myogenic cells."}],"department":[{"_id":"MaHe"}],"year":"2025","citation":{"apa":"Kuhn, K. D., Cho, U. H., & Hetzer, M. (2025). PSME3 regulates migration and differentiation of myoblasts. Life Science Alliance. Embo Press. https://doi.org/10.26508/lsa.202503208","short":"K.D. Kuhn, U.H. Cho, M. Hetzer, Life Science Alliance 8 (2025).","chicago":"Kuhn, Kenneth D, Ukrae H. Cho, and Martin Hetzer. “PSME3 Regulates Migration and Differentiation of Myoblasts.” Life Science Alliance. Embo Press, 2025. https://doi.org/10.26508/lsa.202503208.","ista":"Kuhn KD, Cho UH, Hetzer M. 2025. PSME3 regulates migration and differentiation of myoblasts. Life Science Alliance. 8(9), e202503208.","mla":"Kuhn, Kenneth D., et al. “PSME3 Regulates Migration and Differentiation of Myoblasts.” Life Science Alliance, vol. 8, no. 9, e202503208, Embo Press, 2025, doi:10.26508/lsa.202503208.","ama":"Kuhn KD, Cho UH, Hetzer M. PSME3 regulates migration and differentiation of myoblasts. Life Science Alliance. 2025;8(9). doi:10.26508/lsa.202503208","ieee":"K. D. Kuhn, U. H. Cho, and M. Hetzer, “PSME3 regulates migration and differentiation of myoblasts,” Life Science Alliance, vol. 8, no. 9. Embo Press, 2025."},"publication_identifier":{"eissn":["2575-1077"]},"language":[{"iso":"eng"}],"DOAJ_listed":"1","article_processing_charge":"Yes","date_updated":"2025-12-30T09:17:55Z","isi":1,"title":"PSME3 regulates migration and differentiation of myoblasts","author":[{"full_name":"Kuhn, Kenneth D","last_name":"Kuhn","first_name":"Kenneth D","id":"7deed7e0-0133-11f0-8590-c4600b08d0f4"},{"last_name":"Cho","full_name":"Cho, Ukrae H.","first_name":"Ukrae H."},{"id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","first_name":"Martin W","orcid":"0000-0002-2111-992X","last_name":"Hetzer","full_name":"Hetzer, Martin W"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_place":"publisher"},{"quality_controlled":"1","OA_type":"closed access","publisher":"Fundació de la comunitat valenciana SCITO","date_created":"2025-07-21T08:33:20Z","project":[{"_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A","name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery"}],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"NMR"},{"_id":"LifeSc"}],"doi":"10.29363/nanoge.matsusspring.2025.173","department":[{"_id":"MaIb"},{"_id":"LifeSc"}],"article_number":"173","day":"15","publication":"Proceedings of the MATSUS Spring 2025 Conference","conference":{"start_date":"2025-03-03","end_date":"2025-03-07","name":"MATSUS: Materials for Sustainable Development Conference","location":"Sevilla, Spain"},"_id":"20055","date_published":"2025-03-15T00:00:00Z","oa_version":"None","abstract":[{"lang":"eng","text":"Supercrystals represent three-dimensional orderings of colloidal nanocrystals (NCs), showcasing collective properties in photonics, phononics, and electronics applications.1,2 Recent studies have shown that such assemblies are directly produced during nanocrystal reactions.3–6 However, a fundamental understanding of in situ formed supercrystals that withstand typical NC purification processes remains underexplored, which is important for further use. Herein, we report the reaction precursor-mediated formation of stable PbTe supercrystals. Rationalizing the formation of these assemblies through small-angle x-ray scattering (SAXS) measurements, we unveil their formation mechanism. Our findings reveal that the supercrystal formation occurs in the presence of an excess of lead oleates in the crude solution. It should be noted that the formed supercrystals can be stabilized under specific conditions determined by the lead oleate cluster concentration, content of trioctylphosphine telluride (TOP-Te), NC size and the need of an annealing step at mild conditions. Furthermore, this approach allows for the continuous growth of a secondary phase within the supercrystal; for example in the case of PbTe supercrystals, a PbS shell can be grown on each PbTe NC constituent, resulting in core-shell PbTe-PbS supercrystals. Our work elucidates that reaction precursors play an important role in in situ SC formation and stabilization, implying the possibility of applying this knowledge to other NC reactions."}],"language":[{"iso":"eng"}],"month":"03","article_processing_charge":"No","corr_author":"1","year":"2025","type":"conference","publication_status":"published","citation":{"ama":"Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. In: Proceedings of the MATSUS Spring 2025 Conference. Fundació de la comunitat valenciana SCITO; 2025. doi:10.29363/nanoge.matsusspring.2025.173","ieee":"S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, and M. Ibáñez, “Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case,” in Proceedings of the MATSUS Spring 2025 Conference, Sevilla, Spain, 2025.","apa":"Lee, S., Balazs, D., Horta, S., Rayaroth Puthiyaveettil, A., & Ibáñez, M. (2025). Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. In Proceedings of the MATSUS Spring 2025 Conference. Sevilla, Spain: Fundació de la comunitat valenciana SCITO. https://doi.org/10.29363/nanoge.matsusspring.2025.173","ista":"Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. 2025. Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. Proceedings of the MATSUS Spring 2025 Conference. MATSUS: Materials for Sustainable Development Conference, 173.","short":"S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, M. Ibáñez, in:, Proceedings of the MATSUS Spring 2025 Conference, Fundació de la comunitat valenciana SCITO, 2025.","chicago":"Lee, Seungho, Daniel Balazs, Sharona Horta, Aiswarya Rayaroth Puthiyaveettil, and Maria Ibáñez. “Reaction Precursor-Mediated Formation of Stable Supercrystals in Colloidal Nanocrystal Synthesis: PbTe Case.” In Proceedings of the MATSUS Spring 2025 Conference. Fundació de la comunitat valenciana SCITO, 2025. https://doi.org/10.29363/nanoge.matsusspring.2025.173.","mla":"Lee, Seungho, et al. “Reaction Precursor-Mediated Formation of Stable Supercrystals in Colloidal Nanocrystal Synthesis: PbTe Case.” Proceedings of the MATSUS Spring 2025 Conference, 173, Fundació de la comunitat valenciana SCITO, 2025, doi:10.29363/nanoge.matsusspring.2025.173."},"author":[{"last_name":"Lee","full_name":"Lee, Seungho","id":"BB243B88-D767-11E9-B658-BC13E6697425","first_name":"Seungho","orcid":"0000-0002-6962-8598"},{"last_name":"Balazs","full_name":"Balazs, Daniel","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","orcid":"0000-0001-7597-043X","first_name":"Daniel"},{"full_name":"Horta, Sharona","last_name":"Horta","first_name":"Sharona","id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc"},{"full_name":"Rayaroth Puthiyaveettil, Aiswarya","last_name":"Rayaroth Puthiyaveettil","first_name":"Aiswarya","id":"8aceb01b-8972-11ed-ae7b-d5fe53775add"},{"full_name":"Ibáñez, Maria","last_name":"Ibáñez","orcid":"0000-0001-5013-2843","first_name":"Maria","id":"43C61214-F248-11E8-B48F-1D18A9856A87"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"ISTA and the Werner Siemens Foundation financially supported this work. The Scientific Service Units (SSU) of ISTA supported this research through resources provided by the Electron Microscopy Facility (EMF), NMR Facility and the Lab Support Facility (LSF).","title":"Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case","date_updated":"2026-02-19T09:25:57Z","status":"public"},{"file":[{"file_name":"2025_FrontiersPlantSc_Gallemi.pdf","checksum":"9e6b8b53ba56d4a24a9bd91cf6d2dc58","file_size":3665187,"date_created":"2025-07-31T07:28:54Z","success":1,"file_id":"20093","creator":"dernst","date_updated":"2025-07-31T07:28:54Z","content_type":"application/pdf","access_level":"open_access","relation":"main_file"}],"month":"07","corr_author":"1","volume":16,"type":"journal_article","publication_status":"published","acknowledgement":"The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127). We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler for the efforts to establish immunodetection method.","status":"public","oa":1,"ddc":["580"],"has_accepted_license":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"}],"publisher":"Frontiers Media","project":[{"call_identifier":"FP7","grant_number":"207362","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","name":"Hormonal cross-talk in plant organogenesis"}],"external_id":{"isi":["001530690900001"],"pmid":["40688689"]},"ec_funded":1,"_id":"20080","article_number":"1612366","publication":"Frontiers in Plant Science","day":"04","pmid":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","DOAJ_listed":"1","article_processing_charge":"Yes","publication_identifier":{"eissn":["1664-462X"]},"language":[{"iso":"eng"}],"citation":{"apa":"Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P., Hannezo, E. B., & Benková, E. (2025). Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. Frontiers in Plant Science. Frontiers Media. https://doi.org/10.3389/fpls.2025.1612366","short":"M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B. Hannezo, E. Benková, Frontiers in Plant Science 16 (2025).","chicago":"Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl, Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” Frontiers in Plant Science. Frontiers Media, 2025. https://doi.org/10.3389/fpls.2025.1612366.","ista":"Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB, Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.","mla":"Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” Frontiers in Plant Science, vol. 16, 1612366, Frontiers Media, 2025, doi:10.3389/fpls.2025.1612366.","ama":"Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. Frontiers in Plant Science. 2025;16. doi:10.3389/fpls.2025.1612366","ieee":"M. Gallemi et al., “Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties,” Frontiers in Plant Science, vol. 16. Frontiers Media, 2025."},"year":"2025","OA_place":"publisher","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"last_name":"Gallemi","full_name":"Gallemi, Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4675-6893","first_name":"Marçal"},{"full_name":"Montesinos López, Juan C","last_name":"Montesinos López","first_name":"Juan C","orcid":"0000-0001-9179-6099","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Nikola","id":"18e95355-e05a-11ea-a9c0-8fba1b89e83a","full_name":"Zarevski, Nikola","last_name":"Zarevski"},{"first_name":"Jan","last_name":"Pribyl","full_name":"Pribyl, Jan"},{"last_name":"Skládal","full_name":"Skládal, Petr","first_name":"Petr"},{"full_name":"Hannezo, Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva"}],"title":"Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties","isi":1,"date_updated":"2025-09-30T14:08:22Z","quality_controlled":"1","scopus_import":"1","article_type":"original","file_date_updated":"2025-07-31T07:28:54Z","PlanS_conform":"1","doi":"10.3389/fpls.2025.1612366","OA_type":"gold","date_created":"2025-07-27T22:01:26Z","intvolume":" 16","department":[{"_id":"EdHa"},{"_id":"EvBe"},{"_id":"CaGu"}],"abstract":[{"lang":"eng","text":"Introduction: Acid-growth theory has been postulated in the 70s to explain the rapid elongation of plant cells in response to the hormone auxin. More recently, it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting acidification of the apoplast is the principal mechanism by which auxin and other hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances, the impact of this acidification on the mechanical properties of the cell wall remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate hormone-induced tissue elongation and local changes in cell wall mechanical properties. Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase (PME), along with calcium chelators, we investigate the effect of pectin modification in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification of apoplast is necessary and sufficient to induce cell elongation through promoting cell wall softening. Moreover, we show that enhanced PME activity can induce both cell wall softening or stiffening in extracellular calcium dependent-manner and that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion: Our results confirm a dual role of PME in plant cell elongation. However, further investigation is needed to assess the status of pectin following short- or long-term PME treatments in order to determine if pectin methyl-esterification might promote its degradation as well as the role of PME inhibitors upon PME induction."}],"date_published":"2025-07-04T00:00:00Z"},{"scopus_import":"1","quality_controlled":"1","article_type":"original","issue":"8","file_date_updated":"2025-08-04T06:53:07Z","PlanS_conform":"1","doi":"10.1016/j.celrep.2025.116080","OA_type":"gold","date_created":"2025-08-03T22:01:30Z","intvolume":" 44","department":[{"_id":"PeJo"}],"abstract":[{"lang":"eng","text":"The hippocampus, critical for learning and memory, is dogmatically described as a trisynaptic circuit where dentate gyrus granule cells (GCs), CA3 pyramidal neurons (PNs), and CA1 PNs are serially connected. However, CA3 also forms an autoassociative network, and its PNs have diverse morphologies, intrinsic properties, and GC input levels. How PN subtypes compose this recurrent network is unknown. To determine the synaptic arrangement of identified CA3 PNs, we combine multicellular patch-clamp recording and post hoc morphological analysis in mouse hippocampal slices. PNs can be divided into distinct “superficial” and “deep” subclasses, the latter including previously reported “athorny” cells. Subclasses have distinct input-output transformations and asymmetric connectivity, which is more abundant from superficial to deep PNs, splitting CA3 locally into two parallel recurrent networks. Coincident spontaneous inhibition occurs frequently within but not between subclasses, implying subclass-specific inhibitory innervation. Our results suggest two separately controlled sublayers for parallel information processing in hippocampal CA3."}],"date_published":"2025-08-01T00:00:00Z","DOAJ_listed":"1","article_processing_charge":"Yes","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"language":[{"iso":"eng"}],"citation":{"apa":"Watson, J., Vargas Barroso, V. M., & Jonas, P. M. (2025). Cell-specific wiring routes information flow through hippocampal CA3. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2025.116080","mla":"Watson, Jake, et al. “Cell-Specific Wiring Routes Information Flow through Hippocampal CA3.” Cell Reports, vol. 44, no. 8, 116080, Elsevier, 2025, doi:10.1016/j.celrep.2025.116080.","chicago":"Watson, Jake, Victor M Vargas Barroso, and Peter M Jonas. “Cell-Specific Wiring Routes Information Flow through Hippocampal CA3.” Cell Reports. Elsevier, 2025. https://doi.org/10.1016/j.celrep.2025.116080.","short":"J. Watson, V.M. Vargas Barroso, P.M. Jonas, Cell Reports 44 (2025).","ista":"Watson J, Vargas Barroso VM, Jonas PM. 2025. Cell-specific wiring routes information flow through hippocampal CA3. Cell Reports. 44(8), 116080.","ieee":"J. Watson, V. M. Vargas Barroso, and P. M. Jonas, “Cell-specific wiring routes information flow through hippocampal CA3,” Cell Reports, vol. 44, no. 8. Elsevier, 2025.","ama":"Watson J, Vargas Barroso VM, Jonas PM. Cell-specific wiring routes information flow through hippocampal CA3. Cell Reports. 2025;44(8). doi:10.1016/j.celrep.2025.116080"},"year":"2025","OA_place":"publisher","author":[{"id":"63836096-4690-11EA-BD4E-32803DDC885E","first_name":"Jake","orcid":"0000-0002-8698-3823","last_name":"Watson","full_name":"Watson, Jake"},{"id":"2F55A9DE-F248-11E8-B48F-1D18A9856A87","first_name":"Victor M","last_name":"Vargas Barroso","full_name":"Vargas Barroso, Victor M"},{"last_name":"Jonas","full_name":"Jonas, Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M","orcid":"0000-0001-5001-4804"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","title":"Cell-specific wiring routes information flow through hippocampal CA3","isi":1,"date_updated":"2025-09-30T14:12:02Z","has_accepted_license":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"publisher":"Elsevier","external_id":{"isi":["001544472300002"]},"project":[{"_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","grant_number":"692692","call_identifier":"H2020","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse"},{"name":"Synaptic computations of the hippocampal CA3 circuitry","_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","grant_number":"101026635","call_identifier":"H2020"},{"grant_number":"P36232","_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","name":"Mechanisms of GABA release in hippocampal circuits"},{"grant_number":"754411","call_identifier":"H2020","_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships"}],"_id":"20099","ec_funded":1,"publication":"Cell Reports","article_number":"116080","day":"01","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","file":[{"access_level":"open_access","relation":"main_file","creator":"dernst","content_type":"application/pdf","date_updated":"2025-08-04T06:53:07Z","success":1,"file_id":"20106","file_size":27695214,"date_created":"2025-08-04T06:53:07Z","checksum":"556ff9760661ecd23949d75031043b1f","file_name":"2025_CellReports_Watson.pdf"}],"month":"08","corr_author":"1","volume":44,"publication_status":"published","type":"journal_article","acknowledgement":"We thank Andrea Navas-Olive and Rebecca J. Morse-Mora for critically reading an earlier version of the manuscript. We also thank Florian Marr and Christina Altmutter for excellent technical assistance, Alois Schlögl for programming and data-handling assistance, Todor Asenov for technical support, and Eleftheria Kralli-Beller for manuscript editing. This research was supported by the Scientific Services Units (SSUs) of ISTA. We are particularly grateful for assistance from the Imaging and Optics Facility, Preclinical Facility, Lab Support Facility, and Miba Machine Shop. The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 692692 to P.J., Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635 to J.F.W., and an ISTplus Fellowship through Marie Skłodowska-Curie grant agreement no. 754411 to V.V.-B.), the Austrian Science Fund (P 36232-B, PAT 4178023, and Cluster of Excellence 10.55776/COE16 to P.J.), and a CONACyT fellowship (289638 to V.V.-B.) and was supported by a non-stipendiary EMBO fellowship (ALTF 756–2020 to J.F.W.).","ddc":["570"],"oa":1,"status":"public"},{"acknowledgement":"This work was supported financially by the Austrian Science Fund (FWF, Grant No. I5812-B, “AlloSpace”). This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance Facility and the Lab Support Facility (LSF). We thank Petra Rovò and Margarita Valhondo Falcón for excellent support of the NMR facility.","ddc":["540"],"status":"public","oa":1,"volume":437,"corr_author":"1","file":[{"file_id":"20876","success":1,"date_created":"2025-12-29T14:51:40Z","checksum":"90d50594d8ea9860ac5da41297992847","file_size":2270555,"file_name":"2025_JourMolecularBiology_Rohden.pdf","relation":"main_file","access_level":"open_access","content_type":"application/pdf","date_updated":"2025-12-29T14:51:40Z","creator":"dernst"}],"month":"12","type":"journal_article","publication_status":"published","publication":"Journal of Molecular Biology","day":"01","article_number":"169379","_id":"20258","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"has_accepted_license":"1","external_id":{"isi":["001618289100020"]},"project":[{"_id":"eb9c82eb-77a9-11ec-83b8-aadd536561cf","grant_number":"I05812","name":"AlloSpace. The emergence and mechanisms of allostery"}],"publisher":"Elsevier","acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Rohden, Darja","last_name":"Rohden","first_name":"Darja","id":"81dc668a-19fa-11f0-bf31-d56534059ef3"},{"id":"d42e08e7-f4fc-11eb-af0a-d71e26138f1b","first_name":"Federico","orcid":"0000-0002-9043-136X","last_name":"Napoli","full_name":"Napoli, Federico"},{"full_name":"Kapitonova, Anna","last_name":"Kapitonova","first_name":"Anna","id":"9fb2a840-89e1-11ee-a8b7-cc5c7ba62471"},{"first_name":"Benjamin","id":"71cda2f3-e604-11ee-a1df-da10587eda3f","full_name":"Tatman, Benjamin","last_name":"Tatman"},{"first_name":"Roman J.","last_name":"Lichtenecker","full_name":"Lichtenecker, Roman J."},{"id":"7B541462-FAF6-11E9-A490-E8DFE5697425","first_name":"Paul","orcid":"0000-0002-9350-7606","last_name":"Schanda","full_name":"Schanda, Paul"}],"title":"Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling scheme","OA_place":"publisher","date_updated":"2025-12-29T14:52:17Z","isi":1,"related_material":{"record":[{"status":"public","id":"19956","relation":"research_data"}]},"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1089-8638"],"issn":["0022-2836"]},"article_processing_charge":"Yes (via OA deal)","year":"2025","citation":{"apa":"Rohden, D., Napoli, F., Kapitonova, A., Tatman, B., Lichtenecker, R. J., & Schanda, P. (2025). Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling scheme. Journal of Molecular Biology. Elsevier. https://doi.org/10.1016/j.jmb.2025.169379","mla":"Rohden, Darja, et al. “Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling Scheme.” Journal of Molecular Biology, vol. 437, no. 23, 169379, Elsevier, 2025, doi:10.1016/j.jmb.2025.169379.","ista":"Rohden D, Napoli F, Kapitonova A, Tatman B, Lichtenecker RJ, Schanda P. 2025. Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling scheme. Journal of Molecular Biology. 437(23), 169379.","short":"D. Rohden, F. Napoli, A. Kapitonova, B. Tatman, R.J. Lichtenecker, P. Schanda, Journal of Molecular Biology 437 (2025).","chicago":"Rohden, Darja, Federico Napoli, Anna Kapitonova, Benjamin Tatman, Roman J. Lichtenecker, and Paul Schanda. “Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling Scheme.” Journal of Molecular Biology. Elsevier, 2025. https://doi.org/10.1016/j.jmb.2025.169379.","ieee":"D. Rohden, F. Napoli, A. Kapitonova, B. Tatman, R. J. Lichtenecker, and P. Schanda, “Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling scheme,” Journal of Molecular Biology, vol. 437, no. 23. Elsevier, 2025.","ama":"Rohden D, Napoli F, Kapitonova A, Tatman B, Lichtenecker RJ, Schanda P. Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling scheme. Journal of Molecular Biology. 2025;437(23). doi:10.1016/j.jmb.2025.169379"},"department":[{"_id":"PaSc"}],"date_published":"2025-12-01T00:00:00Z","abstract":[{"text":"The specific introduction of ^1H-^13C or ^1H-^15N moieties into otherwise deuterated proteins holds great potential for high-resolution solution and magic-angle spinning (MAS) NMR studies of protein structure and dynamics. Arginine residues play key roles for example at active sites of enzymes. Taking advantage of a chemically synthesized Arg with a ^13C-^1H2 group in an otherwise deuterated backbone, we demonstrate here the usefulness of proton-detected MAS NMR approaches to probe arginine dynamics. In experiments with crystalline ubiquitin and the 134 kDa tetrameric enzyme malate dehydrogenase we detected a wide range of motions, from sites that are rigid on time scales of at least tens of milliseconds to residues undergoing predominantly nanosecond motions. Spin-relaxation and dipolar-coupling measurements enabled quantitative determination of these dynamics. We observed microsecond dynamics of residue Arg54 in crystalline ubiquitin, whose backbone is known to sample different β-turn conformations on this time scale. The labeling scheme and experiments presented here expand the toolkit for high-resolution proton-detected MAS NMR.","lang":"eng"}],"file_date_updated":"2025-12-29T14:51:40Z","article_type":"original","issue":"23","scopus_import":"1","quality_controlled":"1","intvolume":" 437","date_created":"2025-08-31T22:01:33Z","OA_type":"hybrid","doi":"10.1016/j.jmb.2025.169379","PlanS_conform":"1"},{"title":"Self-generated chemotaxis of mixed cell populations","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Ucar","full_name":"Ucar, Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425","first_name":"Mehmet C","orcid":"0000-0003-0506-4217"},{"full_name":"Zane, Alsberga","last_name":"Zane","first_name":"Alsberga","orcid":"0009-0003-0415-7603","id":"60f7509a-f652-11ea-9d86-b963d6490d7c"},{"full_name":"Alanko, Jonna H","last_name":"Alanko","orcid":"0000-0002-7698-3061","first_name":"Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B"}],"OA_place":"publisher","date_updated":"2026-02-16T12:31:05Z","isi":1,"related_material":{"link":[{"relation":"software","url":"https://github.com/mehmetcanucar/Self-generated-chemotaxis"}]},"publication_identifier":{"issn":["0027-8424"],"eissn":["1091-6490"]},"language":[{"iso":"eng"}],"article_processing_charge":"Yes (in subscription journal)","year":"2025","citation":{"ieee":"M. C. Ucar, A. Zane, J. H. Alanko, M. K. Sixt, and E. B. Hannezo, “Self-generated chemotaxis of mixed cell populations,” Proceedings of the National Academy of Sciences, vol. 122, no. 34. National Academy of Sciences, 2025.","ama":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. 2025;122(34). doi:10.1073/pnas.2504064122","apa":"Ucar, M. C., Zane, A., Alanko, J. H., Sixt, M. K., & Hannezo, E. B. (2025). Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2504064122","mla":"Ucar, Mehmet C., et al. “Self-Generated Chemotaxis of Mixed Cell Populations.” Proceedings of the National Academy of Sciences, vol. 122, no. 34, e2504064122, National Academy of Sciences, 2025, doi:10.1073/pnas.2504064122.","ista":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. 2025. Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. 122(34), e2504064122.","chicago":"Ucar, Mehmet C, Alsberga Zane, Jonna H Alanko, Michael K Sixt, and Edouard B Hannezo. “Self-Generated Chemotaxis of Mixed Cell Populations.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2504064122.","short":"M.C. Ucar, A. Zane, J.H. Alanko, M.K. Sixt, E.B. Hannezo, Proceedings of the National Academy of Sciences 122 (2025)."},"department":[{"_id":"EdHa"},{"_id":"MiSi"}],"date_published":"2025-08-26T00:00:00Z","abstract":[{"text":"Cell and tissue movement in development, cancer invasion, and immune response relies on chemical or mechanical guidance cues. In many systems, this behavior is locally directed by self-generated signaling gradients rather than long-range, prepatterned cues. However, how heterogeneous mixtures of cells interact nonreciprocally and navigate through self-generated gradients remains largely unexplored. Here, we introduce a theoretical framework for the self-organized chemotaxis of heterogeneous cell populations. We find that the relative chemotactic sensitivities of different cell populations control their long-time coupling and comigration dynamics, with boundary conditions such as external cell and attractant reservoirs substantially influencing the migration patterns. Our model predicts an optimal parameter regime that enables robust and colocalized migration. We test our theoretical predictions with in vitro experiments demonstrating the comigration of distinct immune cell populations, and quantitatively reproduce observed migration patterns under wild-type and perturbed conditions. Interestingly, immune cell comigration occurs close to the predicted optimal regime. Finally, we incorporate mechanical interactions into our framework, revealing a nontrivial interplay between chemotactic and mechanical nonreciprocity in driving collective migration. Together, our findings suggest that self-generated chemotaxis is a robust strategy for the navigation of mixed cell populations.","lang":"eng"}],"issue":"34","article_type":"original","file_date_updated":"2025-09-08T07:23:29Z","scopus_import":"1","quality_controlled":"1","OA_type":"hybrid","date_created":"2025-09-07T22:01:32Z","intvolume":" 122","PlanS_conform":"1","doi":"10.1073/pnas.2504064122","acknowledgement":"We thank all members of the M.S. and E.H. groups for stimulating discussions.We thank the Imaging and Optics facility, the Pre-clinical and Lab Support facility of the Institute of Science and Technology Austria for their excellent support and provided resources for the experimental research. In particular, we thank Jack Merrin from the Nanofabrication facility who generated the microfabricated channel used in this study. This work received funding fromt he European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 851288 to E.H.). M.C.U.is funded by a University of Sheffield Strategic Research Fellowship in the Physics of Life and Quantitative Biology.","oa":1,"ddc":["570"],"status":"public","volume":122,"month":"08","file":[{"relation":"main_file","access_level":"open_access","date_updated":"2025-09-08T07:23:29Z","content_type":"application/pdf","creator":"dernst","file_id":"20307","success":1,"file_name":"2025_PNAS_Ucar.pdf","file_size":16069140,"date_created":"2025-09-08T07:23:29Z","checksum":"b36abd92673b6d76376fc9434bad52cc"}],"corr_author":"1","publication_status":"published","type":"journal_article","publication":"Proceedings of the National Academy of Sciences","article_number":"e2504064122","day":"26","_id":"20289","ec_funded":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","pmid":1,"has_accepted_license":"1","publisher":"National Academy of Sciences","project":[{"name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E"}],"external_id":{"isi":["001562181600001"],"pmid":["40838890"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"NanoFab"}]},{"issue":"19","article_type":"original","file_date_updated":"2025-12-30T09:31:11Z","scopus_import":"1","quality_controlled":"1","date_created":"2025-09-07T22:01:33Z","OA_type":"gold","intvolume":" 12","PlanS_conform":"1","doi":"10.1002/admi.202500521","department":[{"_id":"ScWa"},{"_id":"NanoFab"}],"arxiv":1,"date_published":"2025-10-01T00:00:00Z","abstract":[{"text":"Scanning Kelvin probe microscopy (SKPM) is a powerful technique for macroscopic imaging of the electrostatic potential above a surface. Though most often used to image work-function variations of conductive surfaces, it can also be used to probe the surface charge on insulating surfaces. In both cases, relating the measured potential to the underlying signal is non-trivial. Here, general relationships are derived between the measured SKPM voltage and the underlying source, revealing either can be cast as a convolution with an appropriately scaled point spread function (PSF). For charge that exists on a thin insulating layer above a conductor, the PSF has the same shape as what would occur from a work-function variation alone, differing by a simple scaling factor. This relationship is confirmed by: (1) backing it out from finite-element simulations of work-function and charge signals, and (2) experimentally comparing the measured PSF from a small work-function target to that from a small charge spot. This scaling factor is further validated by comparing SKPM charge measurements with Faraday cup measurements for highly charged samples from contact-charging experiments. These results highlight a heretofore unappreciated connection between SKPM voltage and charge signals, offering a rigorous recipe to extract either from experimental data.","lang":"eng"}],"publication_identifier":{"eissn":["2196-7350"]},"language":[{"iso":"eng"}],"DOAJ_listed":"1","article_processing_charge":"Yes","year":"2025","citation":{"apa":"Lenton, I. C., Pertl, F., Shafeek, L. B., & Waitukaitis, S. R. (2025). A duality between surface charge and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces. Wiley. https://doi.org/10.1002/admi.202500521","mla":"Lenton, Isaac C., et al. “A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.” Advanced Materials Interfaces, vol. 12, no. 19, e00521, Wiley, 2025, doi:10.1002/admi.202500521.","chicago":"Lenton, Isaac C, Felix Pertl, Lubuna B Shafeek, and Scott R Waitukaitis. “A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.” Advanced Materials Interfaces. Wiley, 2025. https://doi.org/10.1002/admi.202500521.","ista":"Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. 2025. A duality between surface charge and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces. 12(19), e00521.","short":"I.C. Lenton, F. Pertl, L.B. Shafeek, S.R. Waitukaitis, Advanced Materials Interfaces 12 (2025).","ieee":"I. C. Lenton, F. Pertl, L. B. Shafeek, and S. R. Waitukaitis, “A duality between surface charge and work function in scanning Kelvin probe microscopy,” Advanced Materials Interfaces, vol. 12, no. 19. Wiley, 2025.","ama":"Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. A duality between surface charge and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces. 2025;12(19). doi:10.1002/admi.202500521"},"title":"A duality between surface charge and work function in scanning Kelvin probe microscopy","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Isaac C","orcid":"0000-0002-5010-6984","id":"a550210f-223c-11ec-8182-e2d45e817efb","full_name":"Lenton, Isaac C","last_name":"Lenton"},{"id":"6313aec0-15b2-11ec-abd3-ed67d16139af","first_name":"Felix","orcid":"0000-0003-0463-5794","last_name":"Pertl","full_name":"Pertl, Felix"},{"last_name":"Shafeek","full_name":"Shafeek, Lubuna B","id":"3CD37A82-F248-11E8-B48F-1D18A9856A87","first_name":"Lubuna B","orcid":"0000-0001-7180-6050"},{"orcid":"0000-0002-2299-3176","first_name":"Scott R","id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","full_name":"Waitukaitis, Scott R","last_name":"Waitukaitis"}],"OA_place":"publisher","isi":1,"date_updated":"2025-12-30T09:31:25Z","has_accepted_license":"1","publisher":"Wiley","project":[{"_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","call_identifier":"H2020","grant_number":"949120","name":"Tribocharge: a multi-scale approach to an enduring problem in physics"}],"external_id":{"isi":["001560163400001"],"arxiv":["2506.07187"]},"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"article_number":"e00521","publication":"Advanced Materials Interfaces","day":"01","_id":"20295","ec_funded":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","volume":12,"file":[{"content_type":"application/pdf","date_updated":"2025-12-30T09:31:11Z","creator":"dernst","relation":"main_file","access_level":"open_access","checksum":"906fcc7733be8ce8a83600427b82cd5a","date_created":"2025-12-30T09:31:11Z","file_size":1830117,"file_name":"2025_AdvMaterialsInterfaces_Lenton.pdf","file_id":"20908","success":1}],"month":"10","corr_author":"1","publication_status":"published","type":"journal_article","acknowledgement":"This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (Grant agreement No. 949120). This research was supported by the Scientific Service Units of The Institute of Science and Technology Austria (ISTA) through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing Facility, and Lab Support Facility. The authors wish to thank Dmytro Rak and Juan Carlos Sobarzo for letting us use their equipment. The authors wish to thank Evgeniia Volobueva for advice in preparing PFIB samples. The authors wish to thank the contributions of the whole Waitukaitis group for useful discussions and feedback.","status":"public","oa":1,"ddc":["530"]}]