[{"doi":"10.15479/10.15479/AT-ISTA-19431","month":"03","_id":"19431","oa_version":"Published Version","date_created":"2025-03-20T12:52:47Z","date_updated":"2026-04-07T11:46:32Z","has_accepted_license":"1","type":"dissertation","status":"public","corr_author":"1","alternative_title":["ISTA Thesis"],"citation":{"ista":"Hlavata A. 2025. Regulation of Cytoplasmic RNA Polymerase II. Institute of Science and Technology Austria.","chicago":"Hlavata, Annamaria. “Regulation of Cytoplasmic RNA Polymerase II.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/10.15479/AT-ISTA-19431\">https://doi.org/10.15479/10.15479/AT-ISTA-19431</a>.","short":"A. Hlavata, Regulation of Cytoplasmic RNA Polymerase II, Institute of Science and Technology Austria, 2025.","mla":"Hlavata, Annamaria. <i>Regulation of Cytoplasmic RNA Polymerase II</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/10.15479/AT-ISTA-19431\">10.15479/10.15479/AT-ISTA-19431</a>.","ama":"Hlavata A. Regulation of Cytoplasmic RNA Polymerase II. 2025. doi:<a href=\"https://doi.org/10.15479/10.15479/AT-ISTA-19431\">10.15479/10.15479/AT-ISTA-19431</a>","ieee":"A. Hlavata, “Regulation of Cytoplasmic RNA Polymerase II,” Institute of Science and Technology Austria, 2025.","apa":"Hlavata, A. (2025). <i>Regulation of Cytoplasmic RNA Polymerase II</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/10.15479/AT-ISTA-19431\">https://doi.org/10.15479/10.15479/AT-ISTA-19431</a>"},"date_published":"2025-03-20T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publisher":"Institute of Science and Technology Austria","file_date_updated":"2026-03-20T23:30:04Z","department":[{"_id":"GradSch"},{"_id":"CaBe"}],"publication_status":"published","page":"83","acknowledgement":"I would also like to acknowledge the ISTA Facilities: Lab Support Facility, Protein Services and Electron Microscopy Facility (EMF) and Scientific Computing. EMF for their support during data collections and troubleshooting, especially Valentin. Scientific Computing for solving quickly any issues related with cluster.","abstract":[{"text":"Gene expression is crucial for cell differentiation, development and survival of\r\norganisms. It consists of several steps, starting with transcription that is mediated by\r\nRNA polymerases. These are protein machineries transcribing and producing different\r\ntypes of RNAs. Although, the individual steps of transcription by RNA polymerase II\r\n(Pol II) as well as the structure of Pol II has been extensively studied, surprisingly,\r\nthere is still little known about its regulation and assembly in cytoplasm. Among the\r\nproteins that are important in biogenesis of Pol II are RNA polymerase II associating\r\nproteins (RPAP) and small GPN-loop GTPases (GPN). Both of these protein groups\r\nwere shown to take essential part in assembly of Pol II.\r\nThe aim of this project was to deepen our knowledge in regulation of Pol II in\r\nthe cytoplasm as well as the proteins involved in this process. Techniques of structural\r\nbiology, biochemistry and cell biology were employed to study and characterize cytoplasmic Pol II and its interacting partners.\r\nThis study shows for the first time the structure of cytoplasmic Pol II at high\r\nresolution. The structure also reveals proteins interacting with Pol II in cytoplasm,\r\nnamely GDOWN1, RPAP2. Comparing the structure of cytoplasmic Pol II with transcribing Pol II revealed striking difference in clamp region that is not in closed state.\r\nFurthermore, GDOWN1 and RPAP2 make steric clashes with various transcription\r\nfactors bound to Pol II during different stages of transcription. Even though GPN1 and\r\nGPN3 proteins were not resolved in the cytoplasmic Pol II structure, they are part of\r\nthe complex and their interaction with Pol II was confirmed in vitro. RPAP2 stabilizes\r\nthese proteins on Pol II and several experiments suggest that they interact with the\r\nclamp region. In addition, GDOWN1, RPAP2 and GPNs might keep clamp in open or\r\npartially open state. Based on these results I propose a novel model of regulation of\r\nPol II in cytoplasm. GDOWN1, RPAP2, GPN1 and GPN3 bind to Pol II in cytoplasm\r\nand doing so they can prevent pre-mature binding of DNA or RNA and different transcription factors to Pol II in cytoplasm or before engaging in transcription nucleus.\r\nThis research contributes to the current knowledge of molecular mechanisms\r\nof Pol II regulation in cytoplasm.","lang":"eng"}],"title":"Regulation of Cytoplasmic RNA Polymerase II","supervisor":[{"full_name":"Bernecky, Carrie A","first_name":"Carrie A","id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","last_name":"Bernecky","orcid":"0000-0003-0893-7036"}],"author":[{"id":"36062FEC-F248-11E8-B48F-1D18A9856A87","last_name":"Hlavata","full_name":"Hlavata, Annamaria","first_name":"Annamaria"}],"ddc":["572"],"day":"20","degree_awarded":"PhD","oa":1,"OA_place":"publisher","article_processing_charge":"No","year":"2025","language":[{"iso":"eng"}],"publication_identifier":{"isbn":["978-3-99078-055-8"],"eissn":["2663-337X"]},"file":[{"creator":"ahlavata","file_size":23506747,"date_created":"2025-03-24T12:48:36Z","date_updated":"2026-03-20T23:30:04Z","access_level":"closed","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","embargo_to":"open_access","file_name":"PhD_Thesis_Hlavata_final_submission.docx","relation":"source_file","file_id":"19448","checksum":"b7ddf424ffe95f8c767c53c8bb62d4f3"},{"checksum":"6c5a59c9bac467c3d0b3ffb8ea6d9fd4","file_name":"PhD_Thesis_Hlavata_final_submission_update.pdf","embargo":"2026-03-20","relation":"main_file","file_id":"19449","access_level":"open_access","content_type":"application/pdf","creator":"ahlavata","date_updated":"2026-03-20T23:30:04Z","date_created":"2025-03-24T12:51:10Z","file_size":9478591}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"EM-Fac"},{"_id":"ScienComp"}]},{"project":[{"call_identifier":"H2020","grant_number":"771402","_id":"2649B4DE-B435-11E9-9278-68D0E5697425","name":"Epidemics in ant societies on a chip"}],"_id":"19302","oa_version":"Published Version","month":"02","date_created":"2025-03-06T12:16:54Z","date_updated":"2026-03-02T23:31:14Z","doi":"10.15479/AT-ISTA-19302","type":"dissertation","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"has_accepted_license":"1","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","date_published":"2025-02-24T00:00:00Z","citation":{"ista":"Sartoris L. 2025. The effect of circadian rhythm on organisational immunity of ant colonies. Institute of Science and Technology Austria.","chicago":"Sartoris, Linda. “The Effect of Circadian Rhythm on Organisational Immunity of Ant Colonies.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19302\">https://doi.org/10.15479/AT-ISTA-19302</a>.","short":"L. Sartoris, The Effect of Circadian Rhythm on Organisational Immunity of Ant Colonies, Institute of Science and Technology Austria, 2025.","mla":"Sartoris, Linda. <i>The Effect of Circadian Rhythm on Organisational Immunity of Ant Colonies</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19302\">10.15479/AT-ISTA-19302</a>.","ama":"Sartoris L. The effect of circadian rhythm on organisational immunity of ant colonies. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19302\">10.15479/AT-ISTA-19302</a>","ieee":"L. Sartoris, “The effect of circadian rhythm on organisational immunity of ant colonies,” Institute of Science and Technology Austria, 2025.","apa":"Sartoris, L. (2025). <i>The effect of circadian rhythm on organisational immunity of ant colonies</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19302\">https://doi.org/10.15479/AT-ISTA-19302</a>"},"alternative_title":["ISTA Thesis"],"corr_author":"1","status":"public","ec_funded":1,"file_date_updated":"2026-03-02T23:31:13Z","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","department":[{"_id":"GradSch"},{"_id":"SyCr"}],"publisher":"Institute of Science and Technology Austria","title":"The effect of circadian rhythm on organisational immunity of ant colonies","abstract":[{"text":"Social interaction networks of insect colonies facilitate efficient information exchange and\r\ndemonstrate adaptive changes to mitigate disease transmission. While circadian rhythms\r\ninfluence individual behaviour, their role in shaping colony-level defences against pathogens\r\nremains unexplored. Here, we investigate whether social networks of the black garden ant,\r\nLasius niger, exhibit circadian rhythms and how these rhythms influence disease vulnerability\r\nwhen colonies are exposed to a pathogen during the day or the night.\r\nWe first establish baseline daily variations in activity and network dynamics in pathogen-free\r\ncolonies, revealing constitutive daily fluctuations in disease susceptibility. Subsequently, we\r\nexamine pathogen-induced changes in sanitary care and network dynamics by exposing\r\nforagers to a natural pathogen (Metarhizium brunneum) during either the day or the night.\r\nIndividual pathogen loads were measured after a nine-hour post-exposure period to evaluate\r\ntransmission outcomes.\r\nOur results demonstrate that diurnal ant colonies maintain robust circadian patterns in network\r\nproperties while flexibly adapting to pathogen exposure. Ants upregulate sanitary care\r\nirrespective of exposure timing, prioritising the protection of the valuable colony centre\r\nconsisting of nurses and the queen. These findings underscore the robustness and adaptability\r\nof ant colonies in balancing circadian rhythms with effective social immune responses.","lang":"eng"}],"acknowledgement":"Thank you to the Lab Support Facility at ISTA. Thank you to the European Research Council (ERC) for their funding under the European Union’s Horizon 2020 research and innovation program (ERC Consolidator Grant EPIDEMICSonCHIP, No. 771402, to Sylvia Cremer, and ERC Starting Grant DISEASE, No. 802628, to Nathalie Stroeymeyt).","publication_status":"published","page":"85","OA_type":"closed access","article_processing_charge":"No","degree_awarded":"PhD","oa":1,"author":[{"id":"2B9284CA-F248-11E8-B48F-1D18A9856A87","last_name":"Sartoris","first_name":"Linda","full_name":"Sartoris, Linda"}],"ddc":["577"],"day":"24","supervisor":[{"full_name":"Cremer, Sylvia","first_name":"Sylvia","orcid":"0000-0002-2193-3868","last_name":"Cremer","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87"}],"language":[{"iso":"eng"}],"year":"2025","file":[{"checksum":"7e9466dcf3681454211b74b5107e9f7b","file_id":"19310","relation":"source_file","file_name":"Thesis_Linda_Sartoris.docx","embargo_to":"open_access","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","access_level":"closed","date_created":"2025-03-07T10:16:11Z","date_updated":"2026-02-23T23:30:03Z","file_size":7129583,"creator":"lsartori"},{"file_name":"thesis_Sartoris_for_print.pdf","embargo_to":"open_access","file_id":"19384","relation":"other","checksum":"2ccfcf32f0590bb0ec1a488e606a73f5","creator":"lsartori","description":"for printing purposes only","date_created":"2025-03-11T10:42:20Z","file_size":3199703,"date_updated":"2026-03-02T23:31:13Z","content_type":"application/pdf","access_level":"closed"},{"file_id":"19385","relation":"main_file","embargo":"2026-02-23","file_name":"Thesis_Linda_Sartoris.pdf","checksum":"1d1f3c1279065b1a7f407ff6d1ee1503","date_updated":"2026-02-23T23:30:03Z","file_size":3183186,"date_created":"2025-03-11T10:52:00Z","creator":"lsartori","content_type":"application/pdf","access_level":"open_access"}],"acknowledged_ssus":[{"_id":"LifeSc"}],"publication_identifier":{"eissn":["2663-337X"]}},{"language":[{"iso":"eng"}],"year":"2025","publication_identifier":{"isbn":["978-3-99078-056-5"],"issn":["2663-337X"]},"file":[{"checksum":"1c7573303d8e5f6da3eb03d59055390f","embargo":"2025-09-30","file_name":"2025_Thesis_Cumpelik_corrections_PDFA.pdf","relation":"main_file","file_id":"19457","access_level":"open_access","content_type":"application/pdf","creator":"acumpeli","date_updated":"2025-09-30T22:30:02Z","file_size":11869040,"date_created":"2025-03-25T11:07:55Z"},{"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","access_level":"closed","creator":"acumpeli","date_created":"2025-03-25T11:08:05Z","file_size":20436467,"date_updated":"2025-09-30T22:30:02Z","checksum":"b93265ebd9a53f7a14100d0d48b4ff5b","embargo_to":"open_access","file_name":"2025_Thesis_Cumpelik_corrections.docx","file_id":"19458","relation":"source_file"}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"abstract":[{"text":"Making decisions requires flexibly adapting to changing environments, a process that\r\ndepends on accurately interpreting current contingencies and integrating them with\r\npast experience. Two brain regions are particularly critical for this process, the medial\r\nprefrontal cortex (mPFC) and the hippocampus. Using contextual information from the\r\nhippocampus, the mPFC selects relevant cognitive frameworks and suppresses\r\nirrelevant ones to guide appropriate actions. Several studies have shown that some\r\nmPFC pyramidal neurons become spatially tuned when spatial information is required\r\nto guide goal-directed behavior. However, the role of prefrontal spatial representations\r\nin learning and decision making is not well understood. This work aims to characterize\r\nthe role of mPFC spatial tuning in supporting a contextual association task. Rats were\r\ntrained to learn two cue–location associations on a radial arm maze over multiple days,\r\nwhile we simultaneously recorded from dorsal CA1 of the hippocampus and the\r\nprelimbic area of the mPFC. We describe a subset of spatially tuned hippocampal and\r\nprefrontal pyramidal neurons that “flicker” between multiple spatial representations on\r\ndifferent trials, suggesting dynamic, context-dependent coding. This flickering may\r\nprovide a substrate for how the network reorganizes in response to task demands,\r\nlikely by enabling the flexible evaluation of competing representations. ","lang":"eng"}],"title":"The role of prefrontal spatial coding in supporting a contextual association task","publication_status":"published","page":"96","degree_awarded":"PhD","oa":1,"OA_place":"publisher","article_processing_charge":"No","supervisor":[{"orcid":"0000-0002-5193-4036","id":"3FA14672-F248-11E8-B48F-1D18A9856A87","last_name":"Csicsvari","full_name":"Csicsvari, Jozsef L","first_name":"Jozsef L"}],"author":[{"last_name":"Cumpelik","id":"3F158B32-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1727-6612","full_name":"Cumpelik, Andrea D","first_name":"Andrea D"}],"day":"18","ddc":["612"],"citation":{"ama":"Cumpelik AD. The role of prefrontal spatial coding in supporting a contextual association task. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19456\">10.15479/AT-ISTA-19456</a>","ieee":"A. D. Cumpelik, “The role of prefrontal spatial coding in supporting a contextual association task,” Institute of Science and Technology Austria, 2025.","apa":"Cumpelik, A. D. (2025). <i>The role of prefrontal spatial coding in supporting a contextual association task</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19456\">https://doi.org/10.15479/AT-ISTA-19456</a>","ista":"Cumpelik AD. 2025. The role of prefrontal spatial coding in supporting a contextual association task. Institute of Science and Technology Austria.","chicago":"Cumpelik, Andrea D. “The Role of Prefrontal Spatial Coding in Supporting a Contextual Association Task.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19456\">https://doi.org/10.15479/AT-ISTA-19456</a>.","short":"A.D. Cumpelik, The Role of Prefrontal Spatial Coding in Supporting a Contextual Association Task, Institute of Science and Technology Austria, 2025.","mla":"Cumpelik, Andrea D. <i>The Role of Prefrontal Spatial Coding in Supporting a Contextual Association Task</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19456\">10.15479/AT-ISTA-19456</a>."},"date_published":"2025-02-18T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","corr_author":"1","status":"public","alternative_title":["ISTA Thesis"],"file_date_updated":"2025-09-30T22:30:02Z","department":[{"_id":"GradSch"},{"_id":"JoCs"}],"publisher":"Institute of Science and Technology Austria","oa_version":"Published Version","_id":"19456","month":"02","date_updated":"2026-04-07T12:37:58Z","date_created":"2025-03-25T11:22:38Z","OA_embargo":"6 months","doi":"10.15479/AT-ISTA-19456","has_accepted_license":"1","type":"dissertation","keyword":["neuroscience","decision making","learning","cognitive flexibility","medial prefrontal cortex","hippocampus","electrophysiology"]},{"project":[{"grant_number":"862644","call_identifier":"H2020","name":"Quantum readout techniques and technologies","_id":"237CBA6C-32DE-11EA-91FC-C7463DDC885E"},{"_id":"bdb108fd-d553-11ed-ba76-83dc74a9864f","name":"QUANTUM INFORMATION SYSTEMS BEYOND CLASSICAL CAPABILITIES / P5- Integration of Superconducting Quantum Circuits","grant_number":"F07105"}],"date_created":"2025-04-09T16:44:26Z","date_updated":"2026-06-03T07:16:05Z","_id":"19533","month":"04","oa_version":"Published Version","doi":"10.15479/AT-ISTA-19533","type":"dissertation","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"related_material":{"record":[{"relation":"research_data","id":"18978","status":"public"},{"relation":"part_of_dissertation","id":"19280","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"17183"},{"relation":"part_of_dissertation","id":"13117","status":"public"}]},"keyword":["phase transition","open quantum system","phase diagram","cavity quantum electrodynamics","superconducting qubits","semiclassical physics","quantum optics","josephson junction","parametric converter","phase conjugation","quantum radar","quantum entanglement","correlation","quantum sensing"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"apa":"Sett, R. (2025). <i> Quantum remote sensing and non-equilibrium phase transitions in the microwave regime</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19533\">https://doi.org/10.15479/AT-ISTA-19533</a>","ieee":"R. Sett, “ Quantum remote sensing and non-equilibrium phase transitions in the microwave regime,” Institute of Science and Technology Austria, 2025.","ama":"Sett R.  Quantum remote sensing and non-equilibrium phase transitions in the microwave regime. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19533\">10.15479/AT-ISTA-19533</a>","mla":"Sett, Riya. <i> Quantum Remote Sensing and Non-Equilibrium Phase Transitions in the Microwave Regime</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19533\">10.15479/AT-ISTA-19533</a>.","chicago":"Sett, Riya. “ Quantum Remote Sensing and Non-Equilibrium Phase Transitions in the Microwave Regime.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19533\">https://doi.org/10.15479/AT-ISTA-19533</a>.","short":"R. Sett,  Quantum Remote Sensing and Non-Equilibrium Phase Transitions in the Microwave Regime, Institute of Science and Technology Austria, 2025.","ista":"Sett R. 2025.  Quantum remote sensing and non-equilibrium phase transitions in the microwave regime. Institute of Science and Technology Austria."},"date_published":"2025-04-01T00:00:00Z","alternative_title":["ISTA Thesis"],"ec_funded":1,"status":"public","corr_author":"1","department":[{"_id":"GradSch"},{"_id":"JoFi"}],"file_date_updated":"2025-10-11T22:30:02Z","license":"https://creativecommons.org/licenses/by/4.0/","publisher":"Institute of Science and Technology Austria","title":" Quantum remote sensing and non-equilibrium phase transitions in the microwave regime","abstract":[{"lang":"eng","text":"This thesis explores advancements in quantum remote sensing and non-equilibrium phase\r\ntransitions in the microwave regime, with a focus on dissipative phase transitions and quantumenhanced sensing.\r\nIn the first project, I experimentally studied photon blockade breakdown as a dissipative phase\r\ntransition in a zero-dimensional cavity-qubit system. By defining an appropriate thermodynamic\r\nlimit, we demonstrated that the observed bistability is a genuine signature of a first-order\r\nphase transition in this system. This work provides insight into non-equilibrium quantum\r\ndynamics and phase transitions in driven-dissipative open quantum systems.\r\nThe second project focuses on the experimental realization of a phase-conjugate receiver for\r\nquantum illumination (QI), a quantum sensing protocol that enhances target detection in noisy\r\nenvironments using entangled light. While an ideal spontaneous parametric down-conversion\r\n(SPDC) source and receiver could, in theory, provide up to a 6 dB advantage over classical\r\nillumination, no such ideal receiver exists. Instead, we explore an experimental realization of a\r\nphase-conjugate receiver for QI in the microwave regime at millikelvin temperatures using a\r\nJosephson parametric converter (JPC) as a source of continuous-variable Gaussian entangled\r\nsignal-idler pairs, where a maximum 3 dB advantage is theoretically achievable. We investigate\r\nkey experimental limitations that constrain practical QI performance, contributing to the\r\ndevelopment of quantum-enhanced sensing.\r\nAdditionally, this thesis presents efficient digital signal processing (DSP) techniques implemented in C++ and Python in collaboration with Przemysław Zieliński and Luka Drmić. These\r\nmethods, optimized using the Intel Integrated Performance Primitives (IPP) library, have been\r\nessential in data acquisition, noise filtering, and correlation analysis across multiple research\r\nprojects. Although not real-time, these DSP techniques significantly enhance the accuracy of\r\nquantum measurements.\r\nOverall, this thesis advances quantum-enhanced sensing by establishing the thermodynamic\r\nlimit in a single transmon-cavity system and experimentally exploring a phase-conjugate receiver\r\nfor QI. These findings contribute to quantum metrology, particularly for weak signal detection\r\nand remote sensing in noisy environments.\r\n"}],"acknowledgement":"I acknowledge the generous financial support of the Austrian Science Fund (FWF) via BeyondC\r\n(F7105) and the European Union’s Horizon 2020 research and innovation program (FETopen\r\nQUARTET, Grant Agreement No. 862644), which made this research possible. I also extend\r\nmy sincere appreciation to the MIBA workshop and the Institute of Science and Technology\r\nAustria nanofabrication facility for their technical assistance, which was instrumental in realizing\r\nthis work.","page":"109","publication_status":"published","article_processing_charge":"No","OA_place":"publisher","oa":1,"degree_awarded":"PhD","ddc":["530"],"day":"1","author":[{"last_name":"Sett","id":"2E6D040E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7641-8348","first_name":"Riya","full_name":"Sett, Riya"}],"supervisor":[{"first_name":"Johannes M","full_name":"Fink, Johannes M","orcid":"0000-0001-8112-028X","id":"4B591CBA-F248-11E8-B48F-1D18A9856A87","last_name":"Fink"}],"language":[{"iso":"eng"}],"year":"2025","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"LifeSc"},{"_id":"SSU"}],"file":[{"embargo":"2025-10-11","file_name":"PhD_Thesis_Riya_Sett_pdfa.pdf","file_id":"19538","relation":"main_file","checksum":"ba6cd2289d0141a160a14fc97df1632f","creator":"rsett","file_size":4129208,"date_updated":"2025-10-11T22:30:02Z","date_created":"2025-04-10T11:33:22Z","content_type":"application/pdf","access_level":"open_access"},{"relation":"source_file","file_id":"19539","embargo_to":"open_access","file_name":"PhD Thesis Riya Sett.zip","checksum":"ee63a94cb8f7adf5e766903028b81ed6","date_updated":"2025-10-11T22:30:02Z","date_created":"2025-04-10T11:34:08Z","file_size":6646110,"creator":"rsett","access_level":"closed","content_type":"application/x-zip-compressed"}],"publication_identifier":{"issn":["2663-337X"]}},{"OA_place":"publisher","oa":1,"degree_awarded":"PhD","article_processing_charge":"No","supervisor":[{"full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"day":"23","ddc":["580"],"author":[{"id":"F8660870-D756-11E9-98C5-34DFE5697425","last_name":"Inumella","orcid":"0009-0002-5890-120X","first_name":"Syamala","full_name":"Inumella, Syamala"}],"abstract":[{"lang":"eng","text":"As root epidermal cells progress from a phase of elongation to differentiation, their\r\ncortical microtubule (MT) arrays exhibit a transversal-to-longitudinal reorientation. The\r\nhormone cytokinin, a key regulator of root development, facilitates these cytoskeletal\r\nchanges. However, the molecular mechanisms underlying hormone-mediated MT\r\nreorientation during root development are still unknown. Here, we find that MT reorientation\r\nin root cells differs from the existing model in hypocotyl cells, as it does not rely on MT plusend rescue. We show that cytokinin facilitates MT array reorganization during cell\r\ndifferentiation by promoting katanin’s (KTN1) severing activity, and by modulating KTN1’s\r\nassociation with microtubules. Cytokinin regulates SPIRAL2 (SPR2) in a phosphorylationdependent manner, directing its localization to, and stabilization of, the new MT minus-end\r\ncreated by katanin-mediated severing at crossovers. Notably, our findings suggest that\r\ndynamic and reversible phosphorylation at S579 of SPR2 is crucial for the proper functioning\r\nof the MT severing machinery. Finally, we identify MAP65-1 and CLASP as additional targets\r\nof cytokinin-dependent phosphoregulation. Cytokinin treatment decreases MT-MAP65-1\r\nassociation in elongating cells, likely to expose MTs to KTN1-mediated severing, whereas it\r\nincreases MT-CLASP association to stabilize the growing plus-end. In this way, cytokinin drives\r\nMT reorganization during cell development by simultaneously modulating several\r\nmicrotubule-associated proteins. These results reveal key molecular players in hormonemediated cytoskeletal regulation, and highlight protein phosphorylation as a powerful tool\r\nduring this process."}],"title":"Molecular mechanisms of microtubule reorganization in elongating root epidermal cells","page":"113","publication_status":"published","acknowledgement":"Special thanks to the Plant Facility.","publication_identifier":{"isbn":["978-3-99078-059-6"],"issn":["2663-337X"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"file":[{"checksum":"847ec70b2e40f50e0ddc7b8da201d52c","file_id":"19757","relation":"main_file","file_name":"Final Thesis_Syamala Inumella.pdf","embargo":"2026-05-23","content_type":"application/pdf","access_level":"open_access","date_created":"2025-05-28T11:59:11Z","date_updated":"2026-05-23T22:30:02Z","file_size":8292363,"creator":"sinumell"},{"checksum":"17cdffdae13a5f65bdad9c84e9e0e3bd","embargo_to":"open_access","file_name":"Final Thesis_Syamala Inumella.docx","file_id":"19758","relation":"source_file","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","access_level":"closed","creator":"sinumell","date_created":"2025-05-28T11:59:11Z","file_size":7145703,"date_updated":"2026-05-23T22:30:02Z"}],"language":[{"iso":"eng"}],"year":"2025","has_accepted_license":"1","type":"dissertation","date_created":"2025-05-23T15:21:29Z","date_updated":"2026-06-12T08:34:29Z","oa_version":"Published Version","_id":"19722","month":"05","OA_embargo":"12","doi":"10.15479/AT-ISTA-19722","department":[{"_id":"GradSch"},{"_id":"EvBe"}],"file_date_updated":"2026-05-23T22:30:02Z","publisher":"Institute of Science and Technology Austria","date_published":"2025-05-23T00:00:00Z","citation":{"apa":"Inumella, S. (2025). <i>Molecular mechanisms of microtubule reorganization in elongating root epidermal cells</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19722\">https://doi.org/10.15479/AT-ISTA-19722</a>","ieee":"S. Inumella, “Molecular mechanisms of microtubule reorganization in elongating root epidermal cells,” Institute of Science and Technology Austria, 2025.","ama":"Inumella S. Molecular mechanisms of microtubule reorganization in elongating root epidermal cells. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19722\">10.15479/AT-ISTA-19722</a>","mla":"Inumella, Syamala. <i>Molecular Mechanisms of Microtubule Reorganization in Elongating Root Epidermal Cells</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19722\">10.15479/AT-ISTA-19722</a>.","short":"S. Inumella, Molecular Mechanisms of Microtubule Reorganization in Elongating Root Epidermal Cells, Institute of Science and Technology Austria, 2025.","chicago":"Inumella, Syamala. “Molecular Mechanisms of Microtubule Reorganization in Elongating Root Epidermal Cells.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19722\">https://doi.org/10.15479/AT-ISTA-19722</a>.","ista":"Inumella S. 2025. Molecular mechanisms of microtubule reorganization in elongating root epidermal cells. Institute of Science and Technology Austria."},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","status":"public","corr_author":"1","alternative_title":["ISTA Thesis"]},{"year":"2025","language":[{"iso":"eng"}],"publication_identifier":{"issn":["2663-337X"]},"file":[{"checksum":"8cd7fe3ca990adbcafdece119aa0973d","relation":"main_file","file_id":"19764","file_name":"Thesis_Lehr_PDFA.pdf","embargo":"2025-11-30","access_level":"open_access","content_type":"application/pdf","date_updated":"2025-11-30T23:30:02Z","date_created":"2025-05-30T09:10:22Z","file_size":42879974,"creator":"cchlebak"},{"access_level":"closed","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_updated":"2025-11-30T23:30:02Z","file_size":18731094,"date_created":"2025-05-30T09:31:15Z","creator":"cchlebak","checksum":"0c87dd5fc803450a47b20736b5f86a2f","relation":"source_file","file_id":"19765","embargo_to":"open_access","file_name":"Thesis_Lehr_emptyPages.docx"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"}],"publication_status":"published","page":"129","acknowledgement":"My work would also not have been possible without the Imaging and Optics, the Life Science\r\nand the Preclinical Facility of ISTA. Your support has facilitated my research substantially. I\r\nalso want to thank the Graduate School Office for their never-ending support and their sincere\r\neffort to improve the PhD programme of the ISTA even further.\r\nThis work was supported by the Gesellschaft für Forschungsförderung Niederösterreich\r\nm.b.H. fellowship (SC19-011). Thank you for recognizing the importance of this project.","abstract":[{"lang":"eng","text":"Pattern formation in developing organs is controlled by morphogens. These signalling\r\nmolecules form concentration gradients across tissues, thereby providing positional\r\ninformation that instructs the pattern of cell differentiation. Morphogen gradients are highly\r\ndynamic in space and time. Many factors such as morphogen production, spreading,\r\ndegradation, cellular rearrangements and others could contribute to changes in the gradient\r\nshape, yet how the spatiotemporal signalling dynamics arise in many systems is still unclear.\r\nWe studied the dynamics of morphogen signalling and tissue patterning in the developing\r\nvertebrate neural tube. In this system, neural crest, roof plate and distinct dorsal progenitor\r\nsubtypes are specified in a spatially and temporally ordered manner in response to dorsal-toventral gradients of BMP and WNT signalling activity. How the BMP and WNT gradients are\r\nestablished and interpreted to ensure ordered cell specification is poorly understood.\r\nTo address this question, we developed a 2D embryonic stem cell differentiation system that\r\ncaptures key features of dorsal neural tube development. In this system, differentiated\r\ncolonies display remarkable self-organised pattern formation in response to uniformly\r\napplied BMP ligand. We established a method of differentiating the colonies using\r\nmicrofabricated stencils, which allowed us to control the initial size and shape of colonies\r\nwithout confining cell migration and colony growth. This led to highly reproducible pattern\r\nformation that facilitates quantification.\r\nUsing this approach, we observed striking two-phase temporal dynamics of BMP signalling in\r\nour colonies: a BMP gradient rapidly forms from the periphery to the centre of colonies,\r\nsubsequently disappears and is re-established again in the second phase. By combining our\r\nquantitative data with a data-driven theoretical model, we uncovered a temporal relay\r\nmechanism that underlies this biphasic BMP signalling dynamics. The first signalling phase is\r\ncontrolled by fast tissue-autonomous negative feedback that restricts the duration of the\r\ninitial response to BMP. The early BMP activity gradient moreover controls the spatial\r\norganisation of the cell type pattern: the absence of a first phase results in disordered cell\r\ntype pattern. The second phase is controlled by slow positive regulation of BMP signalling by\r\nthe transcription factor LMX1A, a key regulator of roof plate identity. WNT promotes the\r\nsecond phase of BMP signalling via positive feedback on LMX1A.\r\nAltogether, the mechanism that we uncovered ensures the coupling of sequential\r\ndevelopmental events, making pattern formation spatially and temporally organised.\r\nFurthermore, this mechanism allows the BMP signalling pathway to be reused in different\r\ncontexts – first for the establishment of the neural plate border, and subsequently for dorsal\r\nneural progenitor patterning. Our study supports a general developmental principle in which\r\nmultiple morphogens interact with transcriptional networks resulting in complex\r\nspatiotemporal signalling dynamics that ultimately drive organised pattern formation."}],"title":"Dynamics of morphogen signalling and cell fate decisions in the dorsal neural tube","supervisor":[{"orcid":"0000-0003-4509-4998","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","last_name":"Kicheva","first_name":"Anna","full_name":"Kicheva, Anna"}],"author":[{"orcid":"0000-0001-8703-1093","last_name":"Rus","id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","full_name":"Rus, Stefanie","first_name":"Stefanie"}],"ddc":["570"],"day":"29","degree_awarded":"PhD","OA_place":"publisher","oa":1,"article_processing_charge":"No","corr_author":"1","status":"public","alternative_title":["ISTA Thesis"],"citation":{"ista":"Rus S. 2025. Dynamics of morphogen signalling and cell fate decisions in the dorsal neural tube. Institute of Science and Technology Austria.","mla":"Rus, Stefanie. <i>Dynamics of Morphogen Signalling and Cell Fate Decisions in the Dorsal Neural Tube</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19763\">10.15479/AT-ISTA-19763</a>.","chicago":"Rus, Stefanie. “Dynamics of Morphogen Signalling and Cell Fate Decisions in the Dorsal Neural Tube.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19763\">https://doi.org/10.15479/AT-ISTA-19763</a>.","short":"S. Rus, Dynamics of Morphogen Signalling and Cell Fate Decisions in the Dorsal Neural Tube, Institute of Science and Technology Austria, 2025.","ama":"Rus S. Dynamics of morphogen signalling and cell fate decisions in the dorsal neural tube. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19763\">10.15479/AT-ISTA-19763</a>","apa":"Rus, S. (2025). <i>Dynamics of morphogen signalling and cell fate decisions in the dorsal neural tube</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19763\">https://doi.org/10.15479/AT-ISTA-19763</a>","ieee":"S. Rus, “Dynamics of morphogen signalling and cell fate decisions in the dorsal neural tube,” Institute of Science and Technology Austria, 2025."},"date_published":"2025-05-29T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publisher":"Institute of Science and Technology Austria","file_date_updated":"2025-11-30T23:30:02Z","department":[{"_id":"AnKi"},{"_id":"GradSch"}],"doi":"10.15479/AT-ISTA-19763","_id":"19763","oa_version":"Published Version","month":"05","date_updated":"2026-04-14T09:50:53Z","date_created":"2025-05-30T09:14:58Z","project":[{"name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube","_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A","grant_number":"SC19-011"}],"related_material":{"record":[{"relation":"part_of_dissertation","id":"18601","status":"public"},{"status":"public","id":"17148","relation":"part_of_dissertation"},{"status":"public","id":"18807","relation":"part_of_dissertation"},{"status":"public","relation":"part_of_dissertation","id":"13136"}]},"has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"type":"dissertation"},{"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"has_accepted_license":"1","type":"dissertation","doi":"10.15479/AT-ISTA-19993","month":"07","_id":"19993","oa_version":"Published Version","date_created":"2025-07-10T14:12:20Z","date_updated":"2026-04-07T12:39:58Z","publisher":"Institute of Science and Technology Austria","file_date_updated":"2026-01-15T23:30:03Z","department":[{"_id":"GradSch"},{"_id":"SyCr"}],"status":"public","corr_author":"1","alternative_title":["ISTA Thesis"],"citation":{"chicago":"Strahodinsky, Florian. “Social Immunity in a Tri-Partite Host-Pathogen Relationship.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19993\">https://doi.org/10.15479/AT-ISTA-19993</a>.","short":"F. Strahodinsky, Social Immunity in a Tri-Partite Host-Pathogen Relationship, Institute of Science and Technology Austria, 2025.","mla":"Strahodinsky, Florian. <i>Social Immunity in a Tri-Partite Host-Pathogen Relationship</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19993\">10.15479/AT-ISTA-19993</a>.","ista":"Strahodinsky F. 2025. Social immunity in a tri-partite host-pathogen relationship. Institute of Science and Technology Austria.","ieee":"F. Strahodinsky, “Social immunity in a tri-partite host-pathogen relationship,” Institute of Science and Technology Austria, 2025.","apa":"Strahodinsky, F. (2025). <i>Social immunity in a tri-partite host-pathogen relationship</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19993\">https://doi.org/10.15479/AT-ISTA-19993</a>","ama":"Strahodinsky F. Social immunity in a tri-partite host-pathogen relationship. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19993\">10.15479/AT-ISTA-19993</a>"},"date_published":"2025-07-11T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","supervisor":[{"full_name":"Cremer, Sylvia","first_name":"Sylvia","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","last_name":"Cremer","orcid":"0000-0002-2193-3868"}],"author":[{"last_name":"Strahodinsky","id":"979E35EE-C996-11E9-8C7C-CF13E6697425","first_name":"Florian","full_name":"Strahodinsky, Florian"}],"ddc":["570"],"day":"11","degree_awarded":"PhD","oa":1,"OA_place":"publisher","article_processing_charge":"No","publication_status":"published","page":"138","abstract":[{"text":"Ants are frequently challenged by different pathogens, which they counter with\r\nindividual and collective responses. Usually, the pathogens like fungi or viruses are\r\nsolitary and passive pathogens transmitted from host to host. Here, we use a nematobacterial pathogen complex to study worm-borne disease in black garden ants. These\r\nentomopathogenic nematodes are active parasites with an own behavior and chasing\r\npray.\r\nIn the first chapter, we investigated the basic biology of the host-pathogen relationship.\r\nWe tested different ant life stages and found that adult ants display defense behaviors\r\nand are generally resistant to nematode infection, whereas brood is highly susceptible.\r\nIn the case of worker pupae, we found a slight protective effect of the cocoon. When\r\nlarvae are accompanied by adults, meaning a queen or a group of workers, survival is\r\nsignificantly enhanced. Moreover, we found that nematodes can transmit from infected\r\ncadavers to healthy worker larvae, confirming a transmissible disease in ants. Again,\r\nworker presence significantly reduces transmission risk. In the end, we were also able\r\nto disentangle the pathogen system and investigate the pathogenic effect of the\r\nbacterial and nematode components.\r\nIn the second chapter, we studied the effect of multiple infections in adult queens and\r\nqueen larvae. By multiple exposures in the mode of coinfection and superinfections,\r\nwe wanted to assess the detrimental effect of combined fungal and nematode\r\nexposure to better understand how the pathogens interact with each other in an ant\r\nhost. We found instances where combined exposure lead to higher mortality in a given\r\ntime frame in both, adult queens and queen larvae.\r\nOverall entomopathogenic nematodes are a promising model to study worm infections\r\nin ants which extend our knowledge on collective disease defense.","lang":"eng"}],"title":"Social immunity in a tri-partite host-pathogen relationship","publication_identifier":{"issn":["2663-337X"]},"file":[{"embargo_to":"open_access","file_name":"Thesis_Florian_Strahodinsky_DOCX.docx","file_id":"20021","relation":"source_file","checksum":"df3a02f0d937ea9a3d79d5fb94fff097","creator":"fstrahod","date_created":"2025-07-14T13:18:37Z","file_size":9857392,"date_updated":"2026-01-15T23:30:03Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","access_level":"closed"},{"date_updated":"2026-01-15T23:30:03Z","date_created":"2025-07-14T13:18:38Z","file_size":6439602,"creator":"fstrahod","access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_id":"20022","embargo":"2026-01-15","file_name":"Thesis_Florian_Strahodinsky_PDF.pdf","checksum":"7164c21fe1946e839f7b8acd255ce803"}],"acknowledged_ssus":[{"_id":"LifeSc"}],"year":"2025","language":[{"iso":"eng"}]},{"publisher":"Elsevier","file_date_updated":"2024-02-06T13:56:15Z","department":[{"_id":"SiHi"},{"_id":"RySh"}],"status":"public","corr_author":"1","intvolume":"       112","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2024-01-17T00:00:00Z","citation":{"ama":"Cheung GT, Pauler F, Koppensteiner P, et al. Multipotent progenitors instruct ontogeny of the superior colliculus. <i>Neuron</i>. 2024;112(2):230-246.e11. doi:<a href=\"https://doi.org/10.1016/j.neuron.2023.11.009\">10.1016/j.neuron.2023.11.009</a>","apa":"Cheung, G. T., Pauler, F., Koppensteiner, P., Krausgruber, T., Streicher, C., Schrammel, M., … Hippenmeyer, S. (2024). Multipotent progenitors instruct ontogeny of the superior colliculus. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2023.11.009\">https://doi.org/10.1016/j.neuron.2023.11.009</a>","ieee":"G. T. Cheung <i>et al.</i>, “Multipotent progenitors instruct ontogeny of the superior colliculus,” <i>Neuron</i>, vol. 112, no. 2. Elsevier, p. 230–246.e11, 2024.","ista":"Cheung GT, Pauler F, Koppensteiner P, Krausgruber T, Streicher C, Schrammel M, Özgen NY, Ivec A, Bock C, Shigemoto R, Hippenmeyer S. 2024. Multipotent progenitors instruct ontogeny of the superior colliculus. Neuron. 112(2), 230–246.e11.","mla":"Cheung, Giselle T., et al. “Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus.” <i>Neuron</i>, vol. 112, no. 2, Elsevier, 2024, p. 230–246.e11, doi:<a href=\"https://doi.org/10.1016/j.neuron.2023.11.009\">10.1016/j.neuron.2023.11.009</a>.","short":"G.T. Cheung, F. Pauler, P. Koppensteiner, T. Krausgruber, C. Streicher, M. Schrammel, N.Y. Özgen, A. Ivec, C. Bock, R. Shigemoto, S. Hippenmeyer, Neuron 112 (2024) 230–246.e11.","chicago":"Cheung, Giselle T, Florian Pauler, Peter Koppensteiner, Thomas Krausgruber, Carmen Streicher, Martin Schrammel, Natalie Y Özgen, et al. “Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus.” <i>Neuron</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.neuron.2023.11.009\">https://doi.org/10.1016/j.neuron.2023.11.009</a>."},"related_material":{"link":[{"url":"https://ista.ac.at/en/news/the-pedigree-of-brain-cells/","description":"News on ISTA Website","relation":"press_release"}]},"type":"journal_article","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"doi":"10.1016/j.neuron.2023.11.009","scopus_import":"1","project":[{"_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression","grant_number":"F7805"}],"_id":"12875","month":"01","oa_version":"Published Version","date_created":"2023-04-27T09:41:48Z","date_updated":"2025-12-30T10:54:12Z","volume":112,"quality_controlled":"1","file":[{"checksum":"32b3788f7085cf44a84108d8faaff3ce","file_id":"14944","relation":"main_file","file_name":"2024_Neuron_Cheung.pdf","content_type":"application/pdf","access_level":"open_access","file_size":5942467,"date_updated":"2024-02-06T13:56:15Z","date_created":"2024-02-06T13:56:15Z","creator":"dernst","success":1}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"M-Shop"},{"_id":"LifeSc"},{"_id":"PreCl"}],"isi":1,"publication_identifier":{"issn":["0896-6273"]},"publication":"Neuron","year":"2024","article_type":"original","pmid":1,"external_id":{"pmid":["38096816"],"isi":["001163937900001"]},"language":[{"iso":"eng"}],"author":[{"full_name":"Cheung, Giselle T","first_name":"Giselle T","id":"471195F6-F248-11E8-B48F-1D18A9856A87","last_name":"Cheung","orcid":"0000-0001-8457-2572"},{"full_name":"Pauler, Florian","first_name":"Florian","orcid":"0000-0002-7462-0048","last_name":"Pauler","id":"48EA0138-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Peter","full_name":"Koppensteiner, Peter","last_name":"Koppensteiner","id":"3B8B25A8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3509-1948"},{"last_name":"Krausgruber","full_name":"Krausgruber, Thomas","first_name":"Thomas"},{"full_name":"Streicher, Carmen","first_name":"Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher"},{"last_name":"Schrammel","id":"f13e7cae-e8bd-11ed-841a-96dedf69f46d","full_name":"Schrammel, Martin","first_name":"Martin"},{"full_name":"Özgen, Natalie Y","first_name":"Natalie Y","id":"e68ece33-f6e0-11ea-865d-ae1031dcc090","last_name":"Özgen"},{"last_name":"Ivec","id":"1d144691-e8be-11ed-9b33-bdd3077fad4c","full_name":"Ivec, Alexis","first_name":"Alexis"},{"first_name":"Christoph","full_name":"Bock, Christoph","last_name":"Bock"},{"full_name":"Shigemoto, Ryuichi","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","orcid":"0000-0001-8761-9444"},{"full_name":"Hippenmeyer, Simon","first_name":"Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061"}],"day":"17","ddc":["570"],"issue":"2","article_processing_charge":"Yes (via OA deal)","oa":1,"acknowledgement":"We thank Liqun Luo for his continued support, for providing essential resources for generating Fzd10-CreER mice which were generated in his laboratory, and for comments on the manuscript; W. Zhong for providing Nestin-Cre transgenic mouse line for this study; A. Heger for mouse colony management; R. Beattie and T. Asenov for designing and producing components of acute slice recovery chamber for MADM-CloneSeq experiments; and K. Leopold, J. Rodarte and N. Amberg for initial experiments, technical support and/or assistance. This study was supported by the Scientific Service Units (SSU) of IST Austria through resources provided by the Imaging & Optics Facility (IOF), Laboratory Support Facility (LSF), Miba Machine Shop, and Pre-clinical Facility (PCF). G.C. received funding from European Commission (IST plus postdoctoral fellowship). This work was supported by ISTA institutional\r\nfunds; the Austrian Science Fund Special Research Programmes (FWF SFB F78 Neuro Stem Modulation) to S.H. ","publication_status":"published","page":"230-246.e11","title":"Multipotent progenitors instruct ontogeny of the superior colliculus","abstract":[{"lang":"eng","text":"The superior colliculus (SC) in the mammalian midbrain is essential for multisensory integration and is composed of a rich diversity of excitatory and inhibitory neurons and glia. However, the developmental principles directing the generation of SC cell-type diversity are not understood. Here, we pursued systematic cell lineage tracing in silico and in vivo, preserving full spatial information, using genetic mosaic analysis with double markers (MADM)-based clonal analysis with single-cell sequencing (MADM-CloneSeq). The analysis of clonally related cell lineages revealed that radial glial progenitors (RGPs) in SC are exceptionally multipotent. Individual resident RGPs have the capacity to produce all excitatory and inhibitory SC neuron types, even at the stage of terminal division. While individual clonal units show no pre-defined cellular composition, the establishment of appropriate relative proportions of distinct neuronal types occurs in a PTEN-dependent manner. Collectively, our findings provide an inaugural framework at the single-RGP/-cell level of the mammalian SC ontogeny."}]},{"title":"Imaging brain tissue architecture across millimeter to nanometer scales","abstract":[{"text":"Mapping the complex and dense arrangement of cells and their connectivity in brain tissue demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels at visualizing specific molecules and individual cells but fails to provide tissue context. Here we developed Comprehensive Analysis of Tissues across Scales (CATS), a technology to densely map brain tissue architecture from millimeter regional to nanometer synaptic scales in diverse chemically fixed brain preparations, including rodent and human. CATS uses fixation-compatible extracellular labeling and optical imaging, including stimulated emission depletion or expansion microscopy, to comprehensively delineate cellular structures. It enables three-dimensional reconstruction of single synapses and mapping of synaptic connectivity by identification and analysis of putative synaptic cleft regions. Applying CATS to the mouse hippocampal mossy fiber circuitry, we reconstructed and quantified the synaptic input and output structure of identified neurons. We furthermore demonstrate applicability to clinically derived human tissue samples, including formalin-fixed paraffin-embedded routine diagnostic specimens, for visualizing the cellular architecture of brain tissue in health and disease.","lang":"eng"}],"acknowledgement":"We thank J. Vorlaufer, N. Agudelo-Dueñas, W. Jahr and A. Wartak for microscope maintenance and troubleshooting; C. Kreuzinger, A. Freeman and I. Erber for technical assistance; and M. Tomschik for support with obtaining human samples. We gratefully acknowledge E. Miguel for setting up webKnossos and M. Šuplata for computational support and hardware control. We are grateful to R. Shigemoto and B. Bickel for generous support and M. Sixt and S. Boyd (Stanford University) for discussions and critical reading of the paper. PSD95-HaloTag mice were kindly provided by S. Grant (University of Edinburgh). We acknowledge expert support by Institute of Science and Technology Austria’s scientific computing, imaging and optics, preclinical and lab support facilities and by the Miba machine shop and library. We gratefully acknowledge funding by the following sources: Austrian Science Fund (FWF) grant I3600-B27 (J.G.D.); Austrian Science Fund (FWF) grant DK W1232 (J.G.D. and J.M.M.); Austrian Science Fund (FWF) grant Z 312-B27, Wittgenstein award (P.J.); Austrian Science Fund (FWF) projects I4685-B, I6565-B (SYNABS) and DOC 33-B27 (R.H.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); European Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 715508 – REVERSEAUTISM (G.N.); European Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 692692 – GIANTSYN (P.J.); Marie Skłodowska-Curie Actions Fellowship GA no. 665385 under the EU Horizon 2020 program (J.M.M. and J.L.); and Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635 under the EU Horizon 2020 program (J.F.W.).","publication_status":"published","page":"1051-1064","OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)","oa":1,"OA_place":"publisher","author":[{"full_name":"Michalska, Julia M","first_name":"Julia M","orcid":"0000-0003-3862-1235","id":"443DB6DE-F248-11E8-B48F-1D18A9856A87","last_name":"Michalska"},{"id":"46E28B80-F248-11E8-B48F-1D18A9856A87","last_name":"Lyudchik","first_name":"Julia","full_name":"Lyudchik, Julia"},{"first_name":"Philipp","full_name":"Velicky, Philipp","orcid":"0000-0002-2340-7431","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87","last_name":"Velicky"},{"last_name":"Korinkova","id":"ee3cb6ca-ec98-11ea-ae11-ff703e2254ed","first_name":"Hana","full_name":"Korinkova, Hana"},{"last_name":"Watson","id":"63836096-4690-11EA-BD4E-32803DDC885E","orcid":"0000-0002-8698-3823","full_name":"Watson, Jake","first_name":"Jake"},{"first_name":"Alban","full_name":"Cenameri, Alban","last_name":"Cenameri","id":"9ac8f577-2357-11eb-997a-e566c5550886"},{"orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","first_name":"Christoph M","full_name":"Sommer, Christoph M"},{"id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","last_name":"Amberg","orcid":"0000-0002-3183-8207","first_name":"Nicole","full_name":"Amberg, Nicole"},{"orcid":"0000-0003-2356-9403","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","last_name":"Venturino","first_name":"Alessandro","full_name":"Venturino, Alessandro"},{"first_name":"Karl","full_name":"Roessler, Karl","last_name":"Roessler"},{"last_name":"Czech","full_name":"Czech, Thomas","first_name":"Thomas"},{"last_name":"Höftberger","first_name":"Romana","full_name":"Höftberger, Romana"},{"full_name":"Siegert, Sandra","first_name":"Sandra","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","last_name":"Siegert","orcid":"0000-0001-8635-0877"},{"full_name":"Novarino, Gaia","first_name":"Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","last_name":"Novarino","orcid":"0000-0002-7673-7178"},{"full_name":"Jonas, Peter M","first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","last_name":"Jonas","orcid":"0000-0001-5001-4804"},{"last_name":"Danzl","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8559-3973","first_name":"Johann G","full_name":"Danzl, Johann G"}],"day":"01","ddc":["570"],"pmid":1,"external_id":{"isi":["001065254200001"],"pmid":["37653226"]},"language":[{"iso":"eng"}],"year":"2024","article_type":"original","publication":"Nature Biotechnology","file":[{"relation":"main_file","file_id":"18784","file_name":"2024_NatureBiotech_Michalska.pdf","checksum":"57d5fafb16f02dcb9f7dddb1bd7e2a71","date_created":"2025-01-09T07:48:01Z","file_size":26065165,"date_updated":"2025-01-09T07:48:01Z","creator":"dernst","success":1,"access_level":"open_access","content_type":"application/pdf"}],"acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"isi":1,"publication_identifier":{"eissn":["1546-1696"],"issn":["1087-0156"]},"scopus_import":"1","project":[{"grant_number":"I03600","call_identifier":"FWF","name":"Optical control of synaptic function via adhesion molecules","_id":"265CB4D0-B435-11E9-9278-68D0E5697425"},{"name":"Molecular Drug Targets","_id":"2548AE96-B435-11E9-9278-68D0E5697425","grant_number":"W1232","call_identifier":"FWF"},{"_id":"25C5A090-B435-11E9-9278-68D0E5697425","name":"Synaptic communication in neuronal microcircuits","call_identifier":"FWF","grant_number":"Z00312"},{"grant_number":"LS18-022","_id":"23889792-32DE-11EA-91FC-C7463DDC885E","name":"High content imaging to decode human immune cell interactions in health and allergic disease"},{"_id":"25444568-B435-11E9-9278-68D0E5697425","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","call_identifier":"H2020","grant_number":"715508"},{"name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","grant_number":"692692","call_identifier":"H2020"},{"grant_number":"665385","call_identifier":"H2020","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","name":"Synaptic computations of the hippocampal CA3 circuitry","call_identifier":"H2020","grant_number":"101026635"}],"_id":"14257","month":"07","oa_version":"Published Version","quality_controlled":"1","date_created":"2023-09-03T22:01:15Z","volume":42,"date_updated":"2026-04-14T08:34:35Z","doi":"10.1038/s41587-023-01911-8","type":"journal_article","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"related_material":{"link":[{"relation":"software","url":"https://github.com/danzllab/CATS"}],"record":[{"status":"deleted","id":"18660","relation":"dissertation_contains"},{"id":"13126","relation":"research_data","status":"public"},{"relation":"dissertation_contains","id":"18674","status":"public"}]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"        42","date_published":"2024-07-01T00:00:00Z","citation":{"apa":"Michalska, J. M., Lyudchik, J., Velicky, P., Korinkova, H., Watson, J., Cenameri, A., … Danzl, J. G. (2024). Imaging brain tissue architecture across millimeter to nanometer scales. <i>Nature Biotechnology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41587-023-01911-8\">https://doi.org/10.1038/s41587-023-01911-8</a>","ieee":"J. M. Michalska <i>et al.</i>, “Imaging brain tissue architecture across millimeter to nanometer scales,” <i>Nature Biotechnology</i>, vol. 42. Springer Nature, pp. 1051–1064, 2024.","ama":"Michalska JM, Lyudchik J, Velicky P, et al. Imaging brain tissue architecture across millimeter to nanometer scales. <i>Nature Biotechnology</i>. 2024;42:1051-1064. doi:<a href=\"https://doi.org/10.1038/s41587-023-01911-8\">10.1038/s41587-023-01911-8</a>","mla":"Michalska, Julia M., et al. “Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.” <i>Nature Biotechnology</i>, vol. 42, Springer Nature, 2024, pp. 1051–64, doi:<a href=\"https://doi.org/10.1038/s41587-023-01911-8\">10.1038/s41587-023-01911-8</a>.","chicago":"Michalska, Julia M, Julia Lyudchik, Philipp Velicky, Hana Korinkova, Jake Watson, Alban Cenameri, Christoph M Sommer, et al. “Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.” <i>Nature Biotechnology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41587-023-01911-8\">https://doi.org/10.1038/s41587-023-01911-8</a>.","short":"J.M. Michalska, J. Lyudchik, P. Velicky, H. Korinkova, J. Watson, A. Cenameri, C.M. Sommer, N. Amberg, A. Venturino, K. Roessler, T. Czech, R. Höftberger, S. Siegert, G. Novarino, P.M. Jonas, J.G. Danzl, Nature Biotechnology 42 (2024) 1051–1064.","ista":"Michalska JM, Lyudchik J, Velicky P, Korinkova H, Watson J, Cenameri A, Sommer CM, Amberg N, Venturino A, Roessler K, Czech T, Höftberger R, Siegert S, Novarino G, Jonas PM, Danzl JG. 2024. Imaging brain tissue architecture across millimeter to nanometer scales. Nature Biotechnology. 42, 1051–1064."},"corr_author":"1","status":"public","ec_funded":1,"file_date_updated":"2025-01-09T07:48:01Z","department":[{"_id":"SaSi"},{"_id":"GaNo"},{"_id":"PeJo"},{"_id":"JoDa"},{"_id":"Bio"},{"_id":"RySh"}],"publisher":"Springer Nature"},{"publication":"Nature Structural & Molecular Biology","file":[{"checksum":"bda7bf65d81455480efaed8ca293b0db","file_id":"17307","relation":"main_file","file_name":"2024_NatureStrucBio_Datler.pdf","content_type":"application/pdf","access_level":"open_access","date_created":"2024-07-22T11:27:22Z","file_size":17485494,"date_updated":"2024-07-22T11:27:22Z","success":1,"creator":"dernst"}],"isi":1,"acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"publication_identifier":{"issn":["1545-9993"],"eissn":["1545-9985"]},"external_id":{"isi":["001158144600002"],"pmid":["38316877"]},"pmid":1,"language":[{"iso":"eng"}],"year":"2024","article_type":"original","OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)","oa":1,"OA_place":"publisher","author":[{"first_name":"Julia","full_name":"Datler, Julia","last_name":"Datler","id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3616-8580"},{"id":"1063c618-6f9b-11ec-9123-f912fccded63","last_name":"Hansen","orcid":"0000-0001-7967-2085","full_name":"Hansen, Jesse","first_name":"Jesse"},{"first_name":"Andreas","full_name":"Thader, Andreas","last_name":"Thader","id":"3A18A7B8-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-5621-8100","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl","first_name":"Alois","full_name":"Schlögl, Alois"},{"last_name":"Bauer","id":"0c894dcf-897b-11ed-a09c-8186353224b0","full_name":"Bauer, Lukas W","first_name":"Lukas W"},{"full_name":"Hodirnau, Victor-Valentin","first_name":"Victor-Valentin","last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3904-947X"},{"last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4790-8078","full_name":"Schur, Florian KM","first_name":"Florian KM"}],"day":"01","ddc":["570"],"title":"Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores","APC_amount":"11700 EUR","abstract":[{"lang":"eng","text":"Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remained sparse. While major core proteins have been annotated via indirect experimental evidence, their structures have remained elusive and they could not be assigned to individual core features. Hence, which proteins constitute which layers of the core, such as the palisade layer and the inner core wall, has remained enigmatic. Here we show, using a multi-modal cryo-electron microscopy (cryo-EM) approach in combination with AlphaFold molecular modeling, that trimers formed by the cleavage product of VACV protein A10 are the key component of the palisade layer. This allows us to place previously obtained descriptions of protein interactions within the core wall into perspective and to provide a detailed model of poxvirus core architecture. Importantly, we show that interactions within A10 trimers are likely generalizable over members of orthopox- and parapoxviruses."}],"acknowledgement":"We thank A. Bergthaler (Research Center for Molecular Medicine of the Austrian Academy of Sciences) for providing VACV WR. We thank A. Nicholas and his team at the ISTA proteomics facility, and S. Elefante at the ISTA Scientific Computing facility for their support. We also thank F. Fäßler, D. Porley, T. Muthspiel and other members of the Schur group for support and helpful discussions. We also thank D. Castaño-Díez for support with Dynamo. We thank D. Farrell for his help optimizing the Rosetta protocol to refine the atomic model into the cryo-EM map with symmetry.\r\n\r\nF.K.M.S. acknowledges support from ISTA and EMBO. F.K.M.S. also received support from the Austrian Science Fund (FWF) grant P31445. This publication has been made possible in part by CZI grant DAF2021-234754 and grant https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (funder https://doi.org/10.13039/100014989) awarded to F.K.M.S.\r\n\r\nThis research was also supported by the Scientific Service Units (SSUs) of ISTA through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), and the Electron Microscopy Facility (EMF). We also acknowledge the use of COSMIC45 and Colabfold46.","publication_status":"published","page":"1114-1123","file_date_updated":"2024-07-22T11:27:22Z","department":[{"_id":"FlSc"},{"_id":"ScienComp"},{"_id":"EM-Fac"}],"publisher":"Springer Nature","intvolume":"        31","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2024-07-01T00:00:00Z","citation":{"ista":"Datler J, Hansen J, Thader A, Schlögl A, Bauer LW, Hodirnau V-V, Schur FK. 2024. Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. Nature Structural &#38; Molecular Biology. 31, 1114–1123.","mla":"Datler, Julia, et al. “Multi-Modal Cryo-EM Reveals Trimers of Protein A10 to Form the Palisade Layer in Poxvirus Cores.” <i>Nature Structural &#38; Molecular Biology</i>, vol. 31, Springer Nature, 2024, pp. 1114–23, doi:<a href=\"https://doi.org/10.1038/s41594-023-01201-6\">10.1038/s41594-023-01201-6</a>.","chicago":"Datler, Julia, Jesse Hansen, Andreas Thader, Alois Schlögl, Lukas W Bauer, Victor-Valentin Hodirnau, and Florian KM Schur. “Multi-Modal Cryo-EM Reveals Trimers of Protein A10 to Form the Palisade Layer in Poxvirus Cores.” <i>Nature Structural &#38; Molecular Biology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41594-023-01201-6\">https://doi.org/10.1038/s41594-023-01201-6</a>.","short":"J. Datler, J. Hansen, A. Thader, A. Schlögl, L.W. Bauer, V.-V. Hodirnau, F.K. Schur, Nature Structural &#38; Molecular Biology 31 (2024) 1114–1123.","ama":"Datler J, Hansen J, Thader A, et al. Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. <i>Nature Structural &#38; Molecular Biology</i>. 2024;31:1114-1123. doi:<a href=\"https://doi.org/10.1038/s41594-023-01201-6\">10.1038/s41594-023-01201-6</a>","apa":"Datler, J., Hansen, J., Thader, A., Schlögl, A., Bauer, L. W., Hodirnau, V.-V., &#38; Schur, F. K. (2024). Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. <i>Nature Structural &#38; Molecular Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41594-023-01201-6\">https://doi.org/10.1038/s41594-023-01201-6</a>","ieee":"J. Datler <i>et al.</i>, “Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores,” <i>Nature Structural &#38; Molecular Biology</i>, vol. 31. Springer Nature, pp. 1114–1123, 2024."},"status":"public","corr_author":"1","type":"journal_article","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","keyword":["Molecular Biology","Structural Biology"],"related_material":{"link":[{"url":"https://ista.ac.at/en/news/down-to-the-core-of-poxviruses/","description":"News on ISTA Website","relation":"press_release"}],"record":[{"id":"18766","relation":"dissertation_contains","status":"public"}]},"scopus_import":"1","project":[{"name":"Structural conservation and diversity in retroviral capsid","_id":"26736D6A-B435-11E9-9278-68D0E5697425","grant_number":"P31445","call_identifier":"FWF"}],"_id":"14979","oa_version":"Published Version","month":"07","date_updated":"2026-04-07T12:59:44Z","quality_controlled":"1","volume":31,"date_created":"2024-02-12T09:59:45Z","doi":"10.1038/s41594-023-01201-6"},{"quality_controlled":"1","date_updated":"2025-09-04T12:10:40Z","volume":151,"date_created":"2024-03-03T23:00:50Z","month":"02","_id":"15048","oa_version":"Published Version","project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","grant_number":"742573"},{"grant_number":"25239","name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues","_id":"26B1E39C-B435-11E9-9278-68D0E5697425"}],"scopus_import":"1","doi":"10.1242/dev.202316","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"type":"journal_article","related_material":{"record":[{"status":"public","relation":"research_data","id":"14926"}]},"date_published":"2024-02-01T00:00:00Z","citation":{"apa":"Schauer, A., Pranjic-Ferscha, K., Hauschild, R., &#38; Heisenberg, C.-P. J. (2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.202316\">https://doi.org/10.1242/dev.202316</a>","ieee":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust axis elongation by Nodal-dependent restriction of BMP signaling,” <i>Development</i>, vol. 151, no. 4. The Company of Biologists, pp. 1–18, 2024.","ama":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation by Nodal-dependent restriction of BMP signaling. <i>Development</i>. 2024;151(4):1-18. doi:<a href=\"https://doi.org/10.1242/dev.202316\">10.1242/dev.202316</a>","mla":"Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” <i>Development</i>, vol. 151, no. 4, The Company of Biologists, 2024, pp. 1–18, doi:<a href=\"https://doi.org/10.1242/dev.202316\">10.1242/dev.202316</a>.","chicago":"Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” <i>Development</i>. The Company of Biologists, 2024. <a href=\"https://doi.org/10.1242/dev.202316\">https://doi.org/10.1242/dev.202316</a>.","short":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development 151 (2024) 1–18.","ista":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4), 1–18."},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","intvolume":"       151","ec_funded":1,"corr_author":"1","status":"public","department":[{"_id":"CaHe"},{"_id":"Bio"}],"file_date_updated":"2024-03-04T07:24:43Z","publisher":"The Company of Biologists","abstract":[{"text":"Embryogenesis results from the coordinated activities of different signaling pathways controlling cell fate specification and morphogenesis. In vertebrate gastrulation, both Nodal and BMP signaling play key roles in germ layer specification and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis is still insufficiently understood. Here, we took a reductionist approach using zebrafish embryonic explants to study the coordination of Nodal and BMP signaling for embryo patterning and morphogenesis. We show that Nodal signaling triggers explant elongation by inducing mesendodermal progenitors but also suppressing BMP signaling activity at the site of mesendoderm induction. Consistent with this, ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm intercalations, key processes during explant elongation. Translating these ex vivo observations to the intact embryo showed that, similar to explants, Nodal signaling suppresses the effect of BMP signaling on cell intercalations in the dorsal domain, thus allowing robust embryonic axis elongation. These findings suggest a dual function of Nodal signaling in embryonic axis elongation by both inducing mesendoderm and suppressing BMP effects in the dorsal portion of the mesendoderm.","lang":"eng"}],"title":"Robust axis elongation by Nodal-dependent restriction of BMP signaling","page":"1-18","publication_status":"published","acknowledgement":"We thank Patrick Müller for sharing the chordintt250 mutant zebrafish line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro and Katherine Rogers and members of the Heisenberg lab for discussions, technical advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo for discussions. We thank the Imaging and Optics Facility as well as the Life Science facility at IST Austria for support with microscopy and fish maintenance.\r\nThis work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573 to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience and Technology Austria. ","oa":1,"article_processing_charge":"Yes (via OA deal)","issue":"4","ddc":["570"],"day":"01","author":[{"full_name":"Schauer, Alexandra","first_name":"Alexandra","last_name":"Schauer","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7659-9142"},{"last_name":"Pranjic-Ferscha","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","first_name":"Kornelija","full_name":"Pranjic-Ferscha, Kornelija"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"language":[{"iso":"eng"}],"pmid":1,"external_id":{"pmid":["38372390"],"isi":["001170580200001"]},"article_type":"original","year":"2024","publication":"Development","publication_identifier":{"issn":["0950-1991"],"eissn":["1477-9129"]},"isi":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"file":[{"checksum":"6961ea10012bf0d266681f9628bb8f13","file_name":"2024_Development_Schauer.pdf","file_id":"15050","relation":"main_file","content_type":"application/pdf","access_level":"open_access","creator":"dernst","success":1,"date_created":"2024-03-04T07:24:43Z","file_size":14839986,"date_updated":"2024-03-04T07:24:43Z"}]},{"publisher":"Springer Nature","department":[{"_id":"MaLo"}],"status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","intvolume":"         9","citation":{"ieee":"P. Nußbaum <i>et al.</i>, “Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division,” <i>Nature Microbiology</i>, vol. 9, no. 3. Springer Nature, pp. 698–711, 2024.","apa":"Nußbaum, P., Kureisaite-Ciziene, D., Bellini, D., Van Der Does, C., Kojic, M., Taib, N., … Albers, S. V. (2024). Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division. <i>Nature Microbiology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41564-024-01600-5\">https://doi.org/10.1038/s41564-024-01600-5</a>","ama":"Nußbaum P, Kureisaite-Ciziene D, Bellini D, et al. Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division. <i>Nature Microbiology</i>. 2024;9(3):698-711. doi:<a href=\"https://doi.org/10.1038/s41564-024-01600-5\">10.1038/s41564-024-01600-5</a>","chicago":"Nußbaum, Phillip, Danguole Kureisaite-Ciziene, Dom Bellini, Chris Van Der Does, Marko Kojic, Najwa Taib, Anna Yeates, et al. “Proteins Containing Photosynthetic Reaction Centre Domains Modulate FtsZ-Based Archaeal Cell Division.” <i>Nature Microbiology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41564-024-01600-5\">https://doi.org/10.1038/s41564-024-01600-5</a>.","short":"P. Nußbaum, D. Kureisaite-Ciziene, D. Bellini, C. Van Der Does, M. Kojic, N. Taib, A. Yeates, M. Tourte, S. Gribaldo, M. Loose, J. Löwe, S.V. Albers, Nature Microbiology 9 (2024) 698–711.","mla":"Nußbaum, Phillip, et al. “Proteins Containing Photosynthetic Reaction Centre Domains Modulate FtsZ-Based Archaeal Cell Division.” <i>Nature Microbiology</i>, vol. 9, no. 3, Springer Nature, 2024, pp. 698–711, doi:<a href=\"https://doi.org/10.1038/s41564-024-01600-5\">10.1038/s41564-024-01600-5</a>.","ista":"Nußbaum P, Kureisaite-Ciziene D, Bellini D, Van Der Does C, Kojic M, Taib N, Yeates A, Tourte M, Gribaldo S, Loose M, Löwe J, Albers SV. 2024. Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division. Nature Microbiology. 9(3), 698–711."},"date_published":"2024-03-04T00:00:00Z","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"20741"}]},"type":"journal_article","doi":"10.1038/s41564-024-01600-5","project":[{"_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d","name":"In vitro reconstitution of bacterial cell division","grant_number":"P34607"}],"scopus_import":"1","quality_controlled":"1","date_updated":"2026-04-07T12:27:57Z","date_created":"2024-03-17T23:00:58Z","volume":9,"_id":"15118","month":"03","oa_version":"None","isi":1,"acknowledged_ssus":[{"_id":"LifeSc"}],"publication_identifier":{"eissn":["2058-5276"]},"publication":"Nature Microbiology","article_type":"original","year":"2024","language":[{"iso":"eng"}],"external_id":{"pmid":["38443575"],"isi":["001183270800021"]},"pmid":1,"day":"04","author":[{"full_name":"Nußbaum, Phillip","first_name":"Phillip","last_name":"Nußbaum"},{"full_name":"Kureisaite-Ciziene, Danguole","first_name":"Danguole","last_name":"Kureisaite-Ciziene"},{"first_name":"Dom","full_name":"Bellini, Dom","last_name":"Bellini"},{"first_name":"Chris","full_name":"Van Der Does, Chris","last_name":"Van Der Does"},{"first_name":"Marko","full_name":"Kojic, Marko","orcid":"0000-0001-7244-8128","last_name":"Kojic","id":"73e7ecd4-dc85-11ea-9058-88a16394b160"},{"last_name":"Taib","full_name":"Taib, Najwa","first_name":"Najwa"},{"full_name":"Yeates, Anna","first_name":"Anna","last_name":"Yeates"},{"last_name":"Tourte","first_name":"Maxime","full_name":"Tourte, Maxime"},{"first_name":"Simonetta","full_name":"Gribaldo, Simonetta","last_name":"Gribaldo"},{"first_name":"Martin","full_name":"Loose, Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose","orcid":"0000-0001-7309-9724"},{"last_name":"Löwe","full_name":"Löwe, Jan","first_name":"Jan"},{"full_name":"Albers, Sonja Verena","first_name":"Sonja Verena","last_name":"Albers"}],"issue":"3","article_processing_charge":"No","acknowledgement":"We thank X. Ye (ISTA) for providing the His–SUMO expression plasmid pSVA13429. pCDB302 was a gift from C. Bahl (Addgene plasmid number 113673; http://n2t.net/addgene:113673; RRID Addgene_113673). We thank B. Ahsan, G. Sharov, G. Cannone and S. Chen from the Medical Research Council (MRC) LMB Electron Microscopy Facility for help and support. We thank Scientific Computing at the MRC LMB for their support. We thank L. Trübestein and N. Krasnici of the protein service unit of the ISTA Lab Support Facility for help with the SEC coupled with multi-angle light scattering experiments. We thank D. Grohmann and R. Reichelt from the Archaea Centre at the University of Regensburg for providing the P. furiosus cell material. P.N. and S.-V.A. were supported by a Momentum grant from the Volkswagen (VW) Foundation (grant number 94933). D.K.-C. and D.B. were supported by the VW Stiftung ‘Life?’ programme (to J.L.; grant number Az 96727) and by the MRC, as part of UK Research and Innovation (UKRI), MRC file reference number U105184326 (to J.L.). N.T. and S.G. acknowledge support from the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (grant number ANR-10-LABX-62-IBEID), and the computational and storage services (Maestro cluster) provided by the IT department at Institut Pasteur. M.K. and M.L. were supported by the Austrian Science Fund (FWF) Stand-Alone P34607. For the purpose of open access, the MRC Laboratory of Molecular Biology has applied a CC BY public copyright licence to any author accepted manuscript version arising.","page":"698-711","publication_status":"published","title":"Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division","abstract":[{"lang":"eng","text":"Cell division in all domains of life requires the orchestration of many proteins, but in Archaea most of the machinery remains poorly characterized. Here we investigate the FtsZ-based cell division mechanism in Haloferax volcanii and find proteins containing photosynthetic reaction centre (PRC) barrel domains that play an essential role in archaeal cell division. We rename these proteins cell division protein B 1 (CdpB1) and CdpB2. Depletions and deletions in their respective genes cause severe cell division defects, generating drastically enlarged cells. Fluorescence microscopy of tagged FtsZ1, FtsZ2 and SepF in CdpB1 and CdpB2 mutant strains revealed an unusually disordered divisome that is not organized into a distinct ring-like structure. Biochemical analysis shows that SepF forms a tripartite complex with CdpB1/2 and crystal structures suggest that these two proteins might form filaments, possibly aligning SepF and the FtsZ2 ring during cell division. Overall our results indicate that PRC-domain proteins play essential roles in FtsZ-based cell division in Archaea."}]},{"publisher":"Rockefeller University Press","file_date_updated":"2024-03-25T12:52:04Z","department":[{"_id":"FlSc"},{"_id":"MiSi"},{"_id":"Bio"},{"_id":"EM-Fac"}],"article_number":"e202309125","status":"public","corr_author":"1","ec_funded":1,"intvolume":"       223","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"ama":"Zens B, Fäßler F, Hansen J, et al. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. <i>Journal of Cell Biology</i>. 2024;223(6). doi:<a href=\"https://doi.org/10.1083/jcb.202309125\">10.1083/jcb.202309125</a>","ieee":"B. Zens <i>et al.</i>, “Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix,” <i>Journal of Cell Biology</i>, vol. 223, no. 6. Rockefeller University Press, 2024.","apa":"Zens, B., Fäßler, F., Hansen, J., Hauschild, R., Datler, J., Hodirnau, V.-V., … Schur, F. K. (2024). Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202309125\">https://doi.org/10.1083/jcb.202309125</a>","ista":"Zens B, Fäßler F, Hansen J, Hauschild R, Datler J, Hodirnau V-V, Zheden V, Alanko JH, Sixt MK, Schur FK. 2024. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. Journal of Cell Biology. 223(6), e202309125.","short":"B. Zens, F. Fäßler, J. Hansen, R. Hauschild, J. Datler, V.-V. Hodirnau, V. Zheden, J.H. Alanko, M.K. Sixt, F.K. Schur, Journal of Cell Biology 223 (2024).","chicago":"Zens, Bettina, Florian Fäßler, Jesse Hansen, Robert Hauschild, Julia Datler, Victor-Valentin Hodirnau, Vanessa Zheden, Jonna H Alanko, Michael K Sixt, and Florian KM Schur. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2024. <a href=\"https://doi.org/10.1083/jcb.202309125\">https://doi.org/10.1083/jcb.202309125</a>.","mla":"Zens, Bettina, et al. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” <i>Journal of Cell Biology</i>, vol. 223, no. 6, e202309125, Rockefeller University Press, 2024, doi:<a href=\"https://doi.org/10.1083/jcb.202309125\">10.1083/jcb.202309125</a>."},"date_published":"2024-03-20T00:00:00Z","type":"journal_article","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","doi":"10.1083/jcb.202309125","scopus_import":"1","project":[{"_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","name":"Structure and isoform diversity of the Arp2/3 complex","grant_number":"P33367"},{"grant_number":"E435","_id":"7bd318a1-9f16-11ee-852c-cc9217763180","name":"In Situ Actin Structures via Hybrid Cryo-electron Microscopy"},{"name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","grant_number":"724373","call_identifier":"H2020"},{"_id":"059B463C-7A3F-11EA-A408-12923DDC885E","name":"NÖ-Fonds Preis für die Jungforscherin des Jahres am IST Austria"},{"grant_number":"21317","name":"Spatiotemporal regulation of chemokine-induced signalling in leukocyte chemotaxis","_id":"2615199A-B435-11E9-9278-68D0E5697425"},{"grant_number":"CZI01","name":"CryoMinflux-guided in-situ visual proteomics and structure determination","_id":"62909c6f-2b32-11ec-9570-e1476aab5308"}],"_id":"15146","oa_version":"Published Version","month":"03","quality_controlled":"1","volume":223,"date_updated":"2025-09-04T13:17:16Z","date_created":"2024-03-21T06:45:51Z","file":[{"access_level":"open_access","content_type":"application/pdf","success":1,"creator":"dernst","file_size":11907016,"date_created":"2024-03-25T12:52:04Z","date_updated":"2024-03-25T12:52:04Z","checksum":"90d1984a93660735e506c2a304bc3f73","file_name":"2024_JCB_Zens.pdf","relation":"main_file","file_id":"15188"}],"isi":1,"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"M-Shop"}],"publication_identifier":{"eissn":["1540-8140"],"issn":["0021-9525"]},"publication":"Journal of Cell Biology","year":"2024","article_type":"original","external_id":{"pmid":["38506714"],"isi":["001264190100001"]},"pmid":1,"language":[{"iso":"eng"}],"author":[{"orcid":"0000-0002-9561-1239","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","last_name":"Zens","full_name":"Zens, Bettina","first_name":"Bettina"},{"full_name":"Fäßler, Florian","first_name":"Florian","orcid":"0000-0001-7149-769X","last_name":"Fäßler","id":"404F5528-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jesse","full_name":"Hansen, Jesse","last_name":"Hansen","id":"1063c618-6f9b-11ec-9123-f912fccded63","orcid":"0000-0001-7967-2085"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","first_name":"Robert"},{"orcid":"0000-0002-3616-8580","id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87","last_name":"Datler","full_name":"Datler, Julia","first_name":"Julia"},{"orcid":"0000-0003-3904-947X","id":"3661B498-F248-11E8-B48F-1D18A9856A87","last_name":"Hodirnau","full_name":"Hodirnau, Victor-Valentin","first_name":"Victor-Valentin"},{"orcid":"0000-0002-9438-4783","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","last_name":"Zheden","full_name":"Zheden, Vanessa","first_name":"Vanessa"},{"first_name":"Jonna H","full_name":"Alanko, Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","last_name":"Alanko","orcid":"0000-0002-7698-3061"},{"last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","first_name":"Michael K"},{"orcid":"0000-0003-4790-8078","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM","full_name":"Schur, Florian KM"}],"day":"20","ddc":["570"],"issue":"6","article_processing_charge":"Yes (via OA deal)","oa":1,"acknowledgement":"Open Access funding provided by IST Austria. We thank Armel Nicolas and his team at the ISTA proteomics facility, Alois Schloegl, Stefano Elefante, and colleagues at the ISTA Scientific Computing facility, Tommaso Constanzo and Ludek Lovicar at the Electron Microsocpy Facility (EMF), and Thomas Menner at the Miba Machine shop for their support. We also thank Wanda Kukulski (University of Bern) as well as Darío Porley, Andreas Thader, and other members of the Schur group for helpful discussions. Matt Swulius and Jessica Heebner provided great support in using Dragonfly. We thank Dorotea Fracciolla (Art & Science) for support in figure illustration.\r\n\r\nThis research was supported by the Scientific Service Units of ISTA through resources provided by Scientific Computing, the Lab Support Facility, and the Electron Microscopy Facility. We acknowledge funding support from the following sources: Austrian Science Fund (FWF) grant P33367 (to F.K.M. Schur), the Federation of European Biochemical Societies (to F.K.M. Schur), Niederösterreich (NÖ) Fonds (to B. Zens), FWF grant E435 (to J.M. Hansen), European Research Council under the European Union’s Horizon 2020 research (grant agreement No. 724373) (to M. Sixt), and Jenny and Antti Wihuri Foundation (to J. Alanko). This publication has been made possible in part by CZI grant DAF2021-234754 and grant DOI https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (to F.K.M. Schur).","publication_status":"published","title":"Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix","abstract":[{"lang":"eng","text":"The extracellular matrix (ECM) serves as a scaffold for cells and plays an essential role in regulating numerous cellular processes, including cell migration and proliferation. Due to limitations in specimen preparation for conventional room-temperature electron microscopy, we lack structural knowledge on how ECM components are secreted, remodeled, and interact with surrounding cells. We have developed a 3D-ECM platform compatible with sample thinning by cryo-focused ion beam milling, the lift-out extraction procedure, and cryo-electron tomography. Our workflow implements cell-derived matrices (CDMs) grown on EM grids, resulting in a versatile tool closely mimicking ECM environments. This allows us to visualize ECM for the first time in its hydrated, native context. Our data reveal an intricate network of extracellular fibers, their positioning relative to matrix-secreting cells, and previously unresolved structural entities. Our workflow and results add to the structural atlas of the ECM, providing novel insights into its secretion and assembly."}]},{"author":[{"first_name":"Ivan","full_name":"Kulich, Ivan","last_name":"Kulich","id":"57a1567c-8314-11eb-9063-c9ddc3451a54"},{"id":"07cf4637-baaf-11ee-9227-e1de57d1d69b","last_name":"Schmid","first_name":"Julia","full_name":"Schmid, Julia"},{"id":"e3736151-106c-11ec-b916-c2558e2762c6","last_name":"Teplova","first_name":"Anastasiia","full_name":"Teplova, Anastasiia"},{"first_name":"Linlin","full_name":"Qi, Linlin","orcid":"0000-0001-5187-8401","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","last_name":"Qi"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří"}],"day":"05","ddc":["580"],"article_processing_charge":"Yes","oa":1,"acknowledgement":"The research leading to these results has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme grant agreement No 742985 and Austrian Science Fund (FWF): I3630-775 B25 to J.F. This research was also supported by the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) of IST Austria, namely Tereza Bělinová for her help with the imaging. JS was supported by FemTECH fellowship.","publication_status":"published","title":"Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism","abstract":[{"lang":"eng","text":"Root gravitropic bending represents a fundamental aspect of terrestrial plant physiology. Gravity is perceived by sedimentation of starch-rich plastids (statoliths) to the bottom of the central root cap cells. Following gravity perception, intercellular auxin transport is redirected downwards leading to an asymmetric auxin accumulation at the lower root side causing inhibition of cell expansion, ultimately resulting in downwards bending. How gravity-induced statoliths repositioning is translated into asymmetric auxin distribution remains unclear despite PIN auxin efflux carriers and the Negative Gravitropic Response of roots (NGR) proteins polarize along statolith sedimentation, thus providing a plausible mechanism for auxin flow redirection. In this study, using a functional NGR1-GFP construct, we visualized the NGR1 localization on the statolith surface and plasma membrane (PM) domains in close proximity to the statoliths, correlating with their movements. We determined that NGR1 binding to these PM domains is indispensable for NGR1 functionality and relies on cysteine acylation and adjacent polybasic regions as well as on lipid and sterol PM composition. Detailed timing of the early events following graviperception suggested that both NGR1 repolarization and initial auxin asymmetry precede the visible PIN3 polarization. This discrepancy motivated us to unveil a rapid, NGR-dependent translocation of PIN-activating AGCVIII kinase D6PK towards lower PMs of gravity-perceiving cells, thus providing an attractive model for rapid redirection of auxin fluxes following gravistimulation."}],"file":[{"checksum":"a73a84d3bf97a6d09d24308ca6dd0a0c","relation":"main_file","file_id":"15288","file_name":"2024_eLife_Kulich.pdf","access_level":"open_access","content_type":"application/pdf","date_created":"2024-04-03T13:18:00Z","file_size":11451904,"date_updated":"2024-04-03T13:18:00Z","creator":"dernst","success":1}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"publication_identifier":{"issn":["2050-084X"]},"publication":"eLife","year":"2024","article_type":"original","external_id":{"pmid":["38441122"]},"pmid":1,"language":[{"iso":"eng"}],"keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","General Medicine","General Neuroscience"],"related_material":{"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/beneath-the-surface/"}]},"type":"journal_article","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","doi":"10.7554/elife.91523","scopus_import":"1","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985"},{"call_identifier":"FWF","grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants"}],"_id":"15257","oa_version":"Published Version","month":"03","date_updated":"2025-04-23T07:45:02Z","volume":12,"quality_controlled":"1","date_created":"2024-04-02T11:35:58Z","publisher":"eLife Sciences Publications","file_date_updated":"2024-04-03T13:18:00Z","department":[{"_id":"JiFr"}],"article_number":"91523","status":"public","corr_author":"1","ec_funded":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"        12","DOAJ_listed":"1","date_published":"2024-03-05T00:00:00Z","citation":{"mla":"Kulich, Ivan, et al. “Rapid Translocation of NGR Proteins Driving Polarization of PIN-Activating D6 Protein Kinase during Root Gravitropism.” <i>ELife</i>, vol. 12, 91523, eLife Sciences Publications, 2024, doi:<a href=\"https://doi.org/10.7554/elife.91523\">10.7554/elife.91523</a>.","chicago":"Kulich, Ivan, Julia Schmid, Anastasiia Teplova, Linlin Qi, and Jiří Friml. “Rapid Translocation of NGR Proteins Driving Polarization of PIN-Activating D6 Protein Kinase during Root Gravitropism.” <i>ELife</i>. eLife Sciences Publications, 2024. <a href=\"https://doi.org/10.7554/elife.91523\">https://doi.org/10.7554/elife.91523</a>.","short":"I. Kulich, J. Schmid, A. Teplova, L. Qi, J. Friml, ELife 12 (2024).","ista":"Kulich I, Schmid J, Teplova A, Qi L, Friml J. 2024. Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism. eLife. 12, 91523.","apa":"Kulich, I., Schmid, J., Teplova, A., Qi, L., &#38; Friml, J. (2024). Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.91523\">https://doi.org/10.7554/elife.91523</a>","ieee":"I. Kulich, J. Schmid, A. Teplova, L. Qi, and J. Friml, “Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism,” <i>eLife</i>, vol. 12. eLife Sciences Publications, 2024.","ama":"Kulich I, Schmid J, Teplova A, Qi L, Friml J. Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism. <i>eLife</i>. 2024;12. doi:<a href=\"https://doi.org/10.7554/elife.91523\">10.7554/elife.91523</a>"}},{"year":"2024","article_type":"original","external_id":{"pmid":["38579717"],"isi":["001301584600001"]},"pmid":1,"language":[{"iso":"eng"}],"file":[{"checksum":"22b374fb50a40d380b7686c84258d271","relation":"main_file","file_id":"17452","file_name":"2024_DevelopmentalCell_Hoermayer.pdf","access_level":"open_access","content_type":"application/pdf","date_created":"2024-08-20T11:22:16Z","file_size":5195262,"date_updated":"2024-08-20T11:22:16Z","success":1,"creator":"dernst"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"isi":1,"publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"publication":"Developmental Cell","acknowledgement":"We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder for sharing published material. We thank the Imaging & Optics and Life Science Facilities at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility at DBMV UNIL for providing invaluable assistance. The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985, from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).","publication_status":"published","page":"1333-1344.e4","title":"Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization","abstract":[{"lang":"eng","text":"Plant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predictable tissue deformation after wounding. This deformation causes instant redirection of expansion in the surrounding cells and reorientation of microtubule arrays, ultimately predicting cell division orientation. Microtubules are destabilized under low tension, whereas stretching of cells, either through wounding or external aspiration, immediately induces their polymerization. The higher microtubule abundance in the stretched cell parts leads to the reorientation of microtubule arrays and, ultimately, informs cell division planes. This provides a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical forces for tissue reconstruction and plant architecture."}],"author":[{"first_name":"Lukas","full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926","last_name":"Hörmayer","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Montesinos López, Juan C","first_name":"Juan C","orcid":"0000-0001-9179-6099","last_name":"Montesinos López","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Trozzi, N","first_name":"N","last_name":"Trozzi"},{"last_name":"Spona","id":"b52391fb-f636-11ee-939c-8a8c47552e8a","first_name":"Leonhard","full_name":"Spona, Leonhard"},{"full_name":"Yoshida, Saiko","first_name":"Saiko","last_name":"Yoshida","id":"2E46069C-F248-11E8-B48F-1D18A9856A87"},{"id":"44E59624-F248-11E8-B48F-1D18A9856A87","last_name":"Marhavá","first_name":"Petra","full_name":"Marhavá, Petra"},{"full_name":"Caballero Mancebo, Silvia","first_name":"Silvia","orcid":"0000-0002-5223-3346","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","last_name":"Caballero Mancebo"},{"first_name":"Eva","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","orcid":"0000-0002-8510-9739"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Y","full_name":"Dagdas, Y","last_name":"Dagdas"},{"first_name":"M","full_name":"Majda, M","last_name":"Majda"},{"first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"day":"20","ddc":["570"],"issue":"10","article_processing_charge":"Yes (via OA deal)","oa":1,"corr_author":"1","status":"public","ec_funded":1,"intvolume":"        59","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"ieee":"L. Hörmayer <i>et al.</i>, “Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization,” <i>Developmental Cell</i>, vol. 59, no. 10. Elsevier, p. 1333–1344.e4, 2024.","apa":"Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S., Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>","ama":"Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. 2024;59(10):1333-1344.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>","short":"L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá, S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J. Friml, Developmental Cell 59 (2024) 1333–1344.e4.","chicago":"Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>.","mla":"Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>, vol. 59, no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>.","ista":"Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P, Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. 59(10), 1333–1344.e4."},"date_published":"2024-05-20T00:00:00Z","publisher":"Elsevier","file_date_updated":"2024-08-20T11:22:16Z","department":[{"_id":"JiFr"},{"_id":"EvBe"},{"_id":"CaHe"}],"doi":"10.1016/j.devcel.2024.03.009","scopus_import":"1","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985"},{"call_identifier":"FWF","grant_number":"P29988","_id":"262EF96E-B435-11E9-9278-68D0E5697425","name":"RNA-directed DNA methylation in plant development"}],"month":"05","_id":"15301","oa_version":"Published Version","date_created":"2024-04-08T12:07:57Z","date_updated":"2025-09-04T13:32:08Z","volume":59,"quality_controlled":"1","related_material":{"link":[{"url":"https://ista.ac.at/en/news/how-plants-heal-wounds/","description":"News on ISTA website","relation":"press_release"}]},"type":"journal_article","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}},{"type":"journal_article","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"related_material":{"record":[{"status":"public","relation":"earlier_version","id":"14591"}]},"scopus_import":"1","project":[{"name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","call_identifier":"H2020"},{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"_id":"15330","oa_version":"Published Version","month":"04","date_created":"2024-04-19T09:54:59Z","quality_controlled":"1","date_updated":"2025-09-04T13:49:45Z","volume":137,"doi":"10.1242/jcs.261720","file_date_updated":"2025-01-09T08:41:16Z","department":[{"_id":"MaLo"},{"_id":"JiFr"},{"_id":"CaBe"}],"publisher":"The Company of Biologists","intvolume":"       137","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2024-04-01T00:00:00Z","citation":{"ama":"Gnyliukh N, Johnson AJ, Nagel M, et al. Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. <i>Journal of Cell Science</i>. 2024;137(8). doi:<a href=\"https://doi.org/10.1242/jcs.261720\">10.1242/jcs.261720</a>","apa":"Gnyliukh, N., Johnson, A. J., Nagel, M., Monzer, A., Babic, D., Hlavata, A., … Friml, J. (2024). Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.261720\">https://doi.org/10.1242/jcs.261720</a>","ieee":"N. Gnyliukh <i>et al.</i>, “Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana,” <i>Journal of Cell Science</i>, vol. 137, no. 8. The Company of Biologists, 2024.","ista":"Gnyliukh N, Johnson AJ, Nagel M, Monzer A, Babic D, Hlavata A, Alotaibi S, Isono E, Loose M, Friml J. 2024. Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana. Journal of Cell Science. 137(8), jcs. 261720.","mla":"Gnyliukh, Nataliia, et al. “Role of Dynamin-Related Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Arabidopsis Thaliana.” <i>Journal of Cell Science</i>, vol. 137, no. 8, jcs. 261720, The Company of Biologists, 2024, doi:<a href=\"https://doi.org/10.1242/jcs.261720\">10.1242/jcs.261720</a>.","short":"N. Gnyliukh, A.J. Johnson, M. Nagel, A. Monzer, D. Babic, A. Hlavata, S. Alotaibi, E. Isono, M. Loose, J. Friml, Journal of Cell Science 137 (2024).","chicago":"Gnyliukh, Nataliia, Alexander J Johnson, MK Nagel, Aline Monzer, David Babic, Annamaria Hlavata, SS Alotaibi, E Isono, Martin Loose, and Jiří Friml. “Role of Dynamin-Related Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Arabidopsis Thaliana.” <i>Journal of Cell Science</i>. The Company of Biologists, 2024. <a href=\"https://doi.org/10.1242/jcs.261720\">https://doi.org/10.1242/jcs.261720</a>."},"article_number":"jcs.261720","status":"public","corr_author":"1","ec_funded":1,"OA_type":"hybrid","article_processing_charge":"Yes (via OA deal)","OA_place":"publisher","oa":1,"author":[{"orcid":"0000-0002-2198-0509","last_name":"Gnyliukh","id":"390C1120-F248-11E8-B48F-1D18A9856A87","first_name":"Nataliia","full_name":"Gnyliukh, Nataliia"},{"full_name":"Johnson, Alexander J","first_name":"Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson"},{"full_name":"Nagel, MK","first_name":"MK","last_name":"Nagel"},{"first_name":"Aline","full_name":"Monzer, Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer"},{"id":"db566d23-f6e0-11ea-865d-e6f270e968e7","last_name":"Babic","full_name":"Babic, David","first_name":"David"},{"full_name":"Hlavata, Annamaria","first_name":"Annamaria","last_name":"Hlavata","id":"36062FEC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Alotaibi, SS","first_name":"SS","last_name":"Alotaibi"},{"first_name":"E","full_name":"Isono, E","last_name":"Isono"},{"full_name":"Loose, Martin","first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose","orcid":"0000-0001-7309-9724"},{"full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"ddc":["570"],"day":"01","issue":"8","title":"Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana","abstract":[{"text":"Clathrin-mediated endocytosis (CME) is vital for the regulation of plant growth and development by controlling plasma membrane protein composition and cargo uptake. CME relies on the precise recruitment of regulators for vesicle maturation and release. Homologues of components of mammalian vesicle scission are strong candidates to be part of the scission machinery in plants, but the precise roles of these proteins in this process are not fully understood. Here, we characterised the roles of Plant Dynamin-Related Proteins 2 (DRP2s) and SH3-domain containing protein 2 (SH3P2), the plant homologue to Dynamins’ recruiters, like Endophilin and Amphiphysin, in the CME by combining high-resolution imaging of endocytic events in vivo and characterisation of the purified proteins in vitro. Although DRP2s and SH3P2 arrive similarly late during CME and physically interact, genetic analysis of the sh3p123 triple-mutant and complementation assays with non-SH3P2-interacting DRP2 variants suggests that SH3P2 does not directly recruit DRP2s to the site of endocytosis. These observations imply that despite the presence of many well-conserved endocytic components, plants have acquired a distinct mechanism for CME.","lang":"eng"}],"acknowledgement":"Nataliia Gnyliukh was partially funded by the European Union’s Horizon 2020 research and\r\ninnovation program (2018-2020) under the Marie Sklodowska-Curie Grant (agreement no.\r\n665385). Taif University Researchers Supporting Project: TURSP-HC2022/02. and Austrian\r\nScience Fund (FWF): I 6123-B.We thank Prof. Eileen Lafer and Liping Wang for their suggestions regarding the optimisation of protein expression and purification. We thank Prof. Sebastian Y. Bednarek for the useful comments and constructive criticism of the project. We thank Maciek Adamowski for providing genetic material. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Electron microscopy (EMF), Lab Support Facility (LSF) (particularly Dorota Jaworska) and the Bioimaging Facility (BIF).","publication_status":"published","publication":"Journal of Cell Science","file":[{"file_id":"18792","relation":"main_file","file_name":"2024_JourCellScience_Gnyliukh.pdf","checksum":"6dc023f0cc7052ad3cf0a42589d2e30f","file_size":25845948,"date_updated":"2025-01-09T08:41:16Z","date_created":"2025-01-09T08:41:16Z","creator":"dernst","success":1,"content_type":"application/pdf","access_level":"open_access"}],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"Bio"}],"isi":1,"publication_identifier":{"eissn":["1477-9137"],"issn":["0021-9533"]},"external_id":{"isi":["001266917100005"],"pmid":["38506228"]},"pmid":1,"language":[{"iso":"eng"}],"year":"2024","article_type":"original"},{"related_material":{"record":[{"status":"public","id":"20415","relation":"dissertation_contains"}]},"has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"type":"journal_article","doi":"10.1016/j.cej.2024.151405","_id":"15357","oa_version":"Published Version","month":"06","date_created":"2024-05-05T22:01:03Z","quality_controlled":"1","volume":490,"date_updated":"2026-04-07T11:52:31Z","scopus_import":"1","project":[{"_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A","name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery"}],"publisher":"Elsevier","file_date_updated":"2025-01-09T09:24:29Z","department":[{"_id":"MaIb"}],"corr_author":"1","status":"public","article_number":"151405","date_published":"2024-06-15T00:00:00Z","citation":{"ieee":"Y. Liu <i>et al.</i>, “Enhancing thermoelectric performance of solutionpProcessed polycrystalline SnSe with PbSe nanocrystals,” <i>Chemical Engineering Journal</i>, vol. 490. Elsevier, 2024.","apa":"Liu, Y., Lee, S., Fiedler, C.,  Spadaro, M. C., Chang, C., Li, M., … Ibáñez, M. (2024). Enhancing thermoelectric performance of solutionpProcessed polycrystalline SnSe with PbSe nanocrystals. <i>Chemical Engineering Journal</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cej.2024.151405\">https://doi.org/10.1016/j.cej.2024.151405</a>","ama":"Liu Y, Lee S, Fiedler C, et al. Enhancing thermoelectric performance of solutionpProcessed polycrystalline SnSe with PbSe nanocrystals. <i>Chemical Engineering Journal</i>. 2024;490. doi:<a href=\"https://doi.org/10.1016/j.cej.2024.151405\">10.1016/j.cej.2024.151405</a>","chicago":"Liu, Yu, Seungho Lee, Christine Fiedler, Maria Chiara  Spadaro, Cheng Chang, Mingquan Li, Min Hong, Jordi Arbiol, and Maria Ibáñez. “Enhancing Thermoelectric Performance of SolutionpProcessed Polycrystalline SnSe with PbSe Nanocrystals.” <i>Chemical Engineering Journal</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cej.2024.151405\">https://doi.org/10.1016/j.cej.2024.151405</a>.","short":"Y. Liu, S. Lee, C. Fiedler, M.C.  Spadaro, C. Chang, M. Li, M. Hong, J. Arbiol, M. Ibáñez, Chemical Engineering Journal 490 (2024).","mla":"Liu, Yu, et al. “Enhancing Thermoelectric Performance of SolutionpProcessed Polycrystalline SnSe with PbSe Nanocrystals.” <i>Chemical Engineering Journal</i>, vol. 490, 151405, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.cej.2024.151405\">10.1016/j.cej.2024.151405</a>.","ista":"Liu Y, Lee S, Fiedler C,  Spadaro MC, Chang C, Li M, Hong M, Arbiol J, Ibáñez M. 2024. Enhancing thermoelectric performance of solutionpProcessed polycrystalline SnSe with PbSe nanocrystals. Chemical Engineering Journal. 490, 151405."},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","intvolume":"       490","author":[{"first_name":"Yu","full_name":"Liu, Yu","id":"2A70014E-F248-11E8-B48F-1D18A9856A87","last_name":"Liu","orcid":"0000-0001-7313-6740"},{"last_name":"Lee","id":"BB243B88-D767-11E9-B658-BC13E6697425","orcid":"0000-0002-6962-8598","full_name":"Lee, Seungho","first_name":"Seungho"},{"last_name":"Fiedler","id":"bd3fceba-dc74-11ea-a0a7-c17f71817366","first_name":"Christine","full_name":"Fiedler, Christine"},{"full_name":" Spadaro, Maria Chiara","first_name":"Maria Chiara","last_name":" Spadaro"},{"first_name":"Cheng","full_name":"Chang, Cheng","orcid":"0000-0002-9515-4277","id":"9E331C2E-9F27-11E9-AE48-5033E6697425","last_name":"Chang"},{"full_name":"Li, Mingquan","first_name":"Mingquan","last_name":"Li"},{"first_name":"Min","full_name":"Hong, Min","last_name":"Hong"},{"first_name":"Jordi","full_name":"Arbiol, Jordi","last_name":"Arbiol"},{"last_name":"Ibáñez","id":"43C61214-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5013-2843","full_name":"Ibáñez, Maria","first_name":"Maria"}],"ddc":["540"],"day":"15","OA_place":"publisher","oa":1,"OA_type":"hybrid","article_processing_charge":"Yes (via OA deal)","publication_status":"published","acknowledgement":"The Scientific Service Units (SSU) of ISTA supported this research through resources provided by the Electron Microscopy Facility (EMF), NMR Facility, and the Lab Support Facility (LSF). Y.L., S.L., C.F., C.C. and M.I. acknowledge financial support from ISTA and the Werner Siemens Foundation. Y.L. acknowledges funding from the National Natural Science Foundation of China (NSFC) (Grants No. 22209034), the Innovation and Entrepreneurship Project of Overseas Returnees in Anhui Province (Grant No. 2022LCX002). C.C. acknowledges funding from the National Natural Science Foundation of China (NSFC) (Grants No. 12374023). ICN2 acknowledges funding from Generalitat de Catalunya 2021SGR00457. The authors thank support from the project NANOGEN(PID2020-116093RB-C43), funded by MCIN/ AEI/10.13039/501100011033/ and by “ERDF Away of making Europe”, by the “European Union”. ICN2 is supported by the Severo Ochoaprogram from Spanish MCIN / AEI (Grant No.: CEX2021-001214-S) and is funded by the CERCA Programme / Generalitat de Catalunya. ICN2 is founding member of e-DREAM [70].","abstract":[{"lang":"eng","text":"There is a growing interest in cost-effective polycrystalline SnSe-based thermoelectric (TE) materials, which are able to replace the high performance but mechanically fragile and costly single-crystalline SnSe. In this study, we present a low-temperature solution-based approach to produce SnSe-PbSe nanocomposites with outstanding TE performance. Our method involves combining surfactant-free SnSe particles with oleate-capped PbSe nanocrystals in specific ratios, followed by thermal annealing and consolidation using spark plasma sintering. These nanocomposites are characterized by distinct compositional and structural properties that significantly impact their transport properties. In particular, the addition of oleate-capped PbSe nanocrystals results in: i) a reduction in the electrostatically adsorbed Na at the surface of the SnSe particles; ii) a reduction of Sn vacancies due to alloying with Pb; iii) an increase in grain boundary density; and iv) the formation of PbSnSe secondary phases. Notably, the SnSe-2.5 %PbSe nanocomposites demonstrate a 30 % decrease in thermal conductivity compared to that of the SnSe matrix. This reduction contributes to a maximum figure of merit (zT) of 1.75 at 788 K with a high average zT value of ca. 1.2 in the medium temperature range of 573–773 K. These values represent one of the highest reported in polycrystalline SnSe materials, showcasing the potential of our fabricated SnSe-PbSe nanocomposites for cost-effective TE applications."}],"title":"Enhancing thermoelectric performance of solutionpProcessed polycrystalline SnSe with PbSe nanocrystals","publication_identifier":{"issn":["1385-8947"]},"file":[{"file_id":"18800","relation":"main_file","file_name":"2024_ChemEngineeringJour_Liu.pdf","checksum":"6609232a208b9a89d055a270ef0af1fe","file_size":12233704,"date_updated":"2025-01-09T09:24:29Z","date_created":"2025-01-09T09:24:29Z","creator":"dernst","success":1,"content_type":"application/pdf","access_level":"open_access"}],"isi":1,"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"NMR"},{"_id":"LifeSc"}],"publication":"Chemical Engineering Journal","year":"2024","article_type":"original","external_id":{"isi":["001234835500001"]},"language":[{"iso":"eng"}]},{"publication":"Neuron","isi":1,"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"file":[{"relation":"main_file","file_id":"18798","file_name":"2024_Neuron_RangelGuerrero.pdf","checksum":"de5b18ff293d42bd90e83a193e889844","file_size":9149079,"date_created":"2025-01-09T09:15:31Z","date_updated":"2025-01-09T09:15:31Z","success":1,"creator":"dernst","access_level":"open_access","content_type":"application/pdf"}],"publication_identifier":{"eissn":["1097-4199"],"issn":["0896-6273"]},"language":[{"iso":"eng"}],"pmid":1,"external_id":{"isi":["001300571400001"],"pmid":["38636524"]},"article_type":"original","year":"2024","article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","oa":1,"OA_place":"publisher","day":"19","ddc":["570"],"author":[{"orcid":"0000-0002-8602-4374","id":"4871BCE6-F248-11E8-B48F-1D18A9856A87","last_name":"Rangel Guerrero","first_name":"Dámaris K","full_name":"Rangel Guerrero, Dámaris K"},{"full_name":"Balueva, Kira","first_name":"Kira","last_name":"Balueva"},{"id":"b515be12-ec90-11ea-b966-d0b5e15613d2","last_name":"Barayeu","full_name":"Barayeu, Uladzislau","first_name":"Uladzislau"},{"first_name":"Peter","full_name":"Baracskay, Peter","id":"361CC00E-F248-11E8-B48F-1D18A9856A87","last_name":"Baracskay"},{"first_name":"Igor","full_name":"Gridchyn, Igor","orcid":"0000-0002-1807-1929","last_name":"Gridchyn","id":"4B60654C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Michele","full_name":"Nardin, Michele","last_name":"Nardin","id":"30BD0376-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8849-6570"},{"last_name":"Roth","id":"37BB4FB6-F248-11E8-B48F-1D18A9856A87","first_name":"Chiara N","full_name":"Roth, Chiara N"},{"last_name":"Wulff","first_name":"Peer","full_name":"Wulff, Peer"},{"full_name":"Csicsvari, Jozsef L","first_name":"Jozsef L","id":"3FA14672-F248-11E8-B48F-1D18A9856A87","last_name":"Csicsvari","orcid":"0000-0002-5193-4036"}],"issue":"12","title":"Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning","abstract":[{"text":"Cholecystokinin-expressing interneurons (CCKIs) are hypothesized to shape pyramidal cell-firing patterns and regulate network oscillations and related network state transitions. To directly probe their role in the CA1 region, we silenced their activity using optogenetic and chemogenetic tools in mice. Opto-tagged CCKIs revealed a heterogeneous population, and their optogenetic silencing triggered wide disinhibitory network changes affecting both pyramidal cells and other interneurons. CCKI silencing enhanced pyramidal cell burst firing and altered the temporal coding of place cells: theta phase precession was disrupted, whereas sequence reactivation was enhanced. Chemogenetic CCKI silencing did not alter the acquisition of spatial reference memories on the Morris water maze but enhanced the recall of contextual fear memories and enabled selective recall when similar environments were tested. This work suggests the key involvement of CCKIs in the control of place-cell temporal coding and the formation of contextual memories.","lang":"eng"}],"acknowledgement":"We thank the kind donations from Andrea Varro, Brian Sauer, Edward Boyden, and Peter Jonas. We thank Jago Wallenschus, Kerstin Kronenbitter, and Didier Gremelle for outstanding technical support; Laura Bollepalli for initial viral targeting experiments; Cihan Önal for initial electrophysiology experiments; Yoav Ben-Simon for histological advice; and Anton Nikitenko for contributing to the analysis. We acknowledge support from the Miba Machine Shop, Bioimaging-, Life Science- and Pre-Clinical Facilities at ISTA. This work was supported by the Austrian Science Fund (FWF I3713 to J.C. as part of the FOR 2143 research consortium), the Deutsche Forschungsgemeinschaft (DFG) (WU 503/2-2 to P.W.), and the Medical Research Council, United Kingdom (grant G1100546/2 to P.W.).","page":"2045-2061.e10","publication_status":"published","department":[{"_id":"JoCs"}],"file_date_updated":"2025-01-09T09:15:31Z","publisher":"Cell Press","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","intvolume":"       112","citation":{"apa":"Rangel Guerrero, D. K., Balueva, K., Barayeu, U., Baracskay, P., Gridchyn, I., Nardin, M., … Csicsvari, J. L. (2024). Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning. <i>Neuron</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.neuron.2024.03.019\">https://doi.org/10.1016/j.neuron.2024.03.019</a>","ieee":"D. K. Rangel Guerrero <i>et al.</i>, “Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning,” <i>Neuron</i>, vol. 112, no. 12. Cell Press, p. 2045–2061.e10, 2024.","ama":"Rangel Guerrero DK, Balueva K, Barayeu U, et al. Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning. <i>Neuron</i>. 2024;112(12):2045-2061.e10. doi:<a href=\"https://doi.org/10.1016/j.neuron.2024.03.019\">10.1016/j.neuron.2024.03.019</a>","mla":"Rangel Guerrero, Dámaris K., et al. “Hippocampal Cholecystokinin-Expressing Interneurons Regulate Temporal Coding and Contextual Learning.” <i>Neuron</i>, vol. 112, no. 12, Cell Press, 2024, p. 2045–2061.e10, doi:<a href=\"https://doi.org/10.1016/j.neuron.2024.03.019\">10.1016/j.neuron.2024.03.019</a>.","short":"D.K. Rangel Guerrero, K. Balueva, U. Barayeu, P. Baracskay, I. Gridchyn, M. Nardin, C.N. Roth, P. Wulff, J.L. Csicsvari, Neuron 112 (2024) 2045–2061.e10.","chicago":"Rangel Guerrero, Dámaris K, Kira Balueva, Uladzislau Barayeu, Peter Baracskay, Igor Gridchyn, Michele Nardin, Chiara N Roth, Peer Wulff, and Jozsef L Csicsvari. “Hippocampal Cholecystokinin-Expressing Interneurons Regulate Temporal Coding and Contextual Learning.” <i>Neuron</i>. Cell Press, 2024. <a href=\"https://doi.org/10.1016/j.neuron.2024.03.019\">https://doi.org/10.1016/j.neuron.2024.03.019</a>.","ista":"Rangel Guerrero DK, Balueva K, Barayeu U, Baracskay P, Gridchyn I, Nardin M, Roth CN, Wulff P, Csicsvari JL. 2024. Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning. Neuron. 112(12), 2045–2061.e10."},"date_published":"2024-06-19T00:00:00Z","status":"public","corr_author":"1","type":"journal_article","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"project":[{"_id":"2654F984-B435-11E9-9278-68D0E5697425","name":"Interneuro plasticity during spatial learning","call_identifier":"FWF","grant_number":"I 3713-B27"}],"scopus_import":"1","quality_controlled":"1","date_updated":"2025-09-08T07:26:42Z","date_created":"2024-05-12T22:01:03Z","volume":112,"oa_version":"Published Version","_id":"15381","month":"06","doi":"10.1016/j.neuron.2024.03.019"},{"department":[{"_id":"MaJö"},{"_id":"PreCl"},{"_id":"SiHi"},{"_id":"RySh"},{"_id":"GaNo"}],"license":"https://creativecommons.org/licenses/by-nc/4.0/","file_date_updated":"2024-05-16T09:08:20Z","publisher":"Institute of Science and Technology Austria","citation":{"ieee":"L. Burnett <i>et al.</i>, “Shared behavioural impairments in visual perception and place avoidance across different autism models are driven by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice.” Institute of Science and Technology Austria, 2024.","apa":"Burnett, L., Koppensteiner, P., Symonova, O., Masson, T., Vega Zuniga, T. A., Contreras, X., … Jösch, M. A. (2024). Shared behavioural impairments in visual perception and place avoidance across different autism models are driven by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:15385\">https://doi.org/10.15479/AT:ISTA:15385</a>","ama":"Burnett L, Koppensteiner P, Symonova O, et al. Shared behavioural impairments in visual perception and place avoidance across different autism models are driven by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice. 2024. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:15385\">10.15479/AT:ISTA:15385</a>","chicago":"Burnett, Laura, Peter Koppensteiner, Olga Symonova, Tomas Masson, Tomas A Vega Zuniga, Ximena Contreras, Thomas Rülicke, Ryuichi Shigemoto, Gaia Novarino, and Maximilian A Jösch. “Shared Behavioural Impairments in Visual Perception and Place Avoidance across Different Autism Models Are Driven by Periaqueductal Grey Hypoexcitability in Setd5 Haploinsufficient Mice.” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/AT:ISTA:15385\">https://doi.org/10.15479/AT:ISTA:15385</a>.","short":"L. Burnett, P. Koppensteiner, O. Symonova, T. Masson, T.A. Vega Zuniga, X. Contreras, T. Rülicke, R. Shigemoto, G. Novarino, M.A. Jösch, (2024).","mla":"Burnett, Laura, et al. <i>Shared Behavioural Impairments in Visual Perception and Place Avoidance across Different Autism Models Are Driven by Periaqueductal Grey Hypoexcitability in Setd5 Haploinsufficient Mice</i>. Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:15385\">10.15479/AT:ISTA:15385</a>.","ista":"Burnett L, Koppensteiner P, Symonova O, Masson T, Vega Zuniga TA, Contreras X, Rülicke T, Shigemoto R, Novarino G, Jösch MA. 2024. Shared behavioural impairments in visual perception and place avoidance across different autism models are driven by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:15385\">10.15479/AT:ISTA:15385</a>."},"date_published":"2024-05-15T00:00:00Z","user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","corr_author":"1","status":"public","tmp":{"image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)"},"has_accepted_license":"1","type":"research_data","related_material":{"record":[{"id":"17142","relation":"used_in_publication","status":"public"}]},"keyword":["ASD","periaqueductal gray","perception","behavior","potassium channels"],"date_created":"2024-05-13T15:04:04Z","date_updated":"2025-09-08T07:57:11Z","oa_version":"Published Version","_id":"15385","month":"05","doi":"10.15479/AT:ISTA:15385","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"M-Shop"},{"_id":"LifeSc"},{"_id":"Bio"}],"file":[{"checksum":"9205eb0876f0f08552dbad80d6884b4b","file_name":"PatchClamp.zip","relation":"main_file","file_id":"15396","access_level":"open_access","content_type":"application/zip","creator":"mjoesch","success":1,"file_size":"1149617663","date_created":"2024-05-15T06:09:17Z","date_updated":"2024-05-15T06:09:17Z"},{"success":1,"creator":"mjoesch","file_size":"564903112","date_created":"2024-05-15T06:09:12Z","date_updated":"2024-05-15T06:09:12Z","content_type":"application/zip","access_level":"open_access","file_name":"SiliconProbe.zip","file_id":"15397","relation":"main_file"},{"checksum":"49a807bbab06b5fada38f532e2176e2e","file_name":"WesternBlot.zip","relation":"main_file","file_id":"15398","access_level":"open_access","content_type":"application/zip","success":1,"creator":"mjoesch","date_created":"2024-05-15T06:09:14Z","date_updated":"2024-05-15T06:09:14Z","file_size":"11685703"},{"access_level":"open_access","content_type":"application/zip","file_size":"1335626779","date_updated":"2024-05-15T06:09:38Z","date_created":"2024-05-15T06:09:38Z","creator":"mjoesch","success":1,"checksum":"beeeeaa43770090f3b291209ed6b0623","relation":"main_file","file_id":"15399","file_name":"Behaviour.zip"},{"date_updated":"2024-05-16T09:08:20Z","file_size":18841,"date_created":"2024-05-16T09:08:20Z","success":1,"creator":"mjoesch","access_level":"open_access","content_type":"text/plain","relation":"main_file","file_id":"15400","file_name":"Readme_Data.txt","checksum":"8862ad7719388304d1d19f8e7db8bb00"}],"year":"2024","oa":1,"article_processing_charge":"No","day":"15","ddc":["570"],"author":[{"last_name":"Burnett","id":"3B717F68-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8937-410X","full_name":"Burnett, Laura","first_name":"Laura"},{"last_name":"Koppensteiner","id":"3B8B25A8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3509-1948","full_name":"Koppensteiner, Peter","first_name":"Peter"},{"first_name":"Olga","full_name":"Symonova, Olga","last_name":"Symonova","id":"3C0C7BC6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2012-9947"},{"full_name":"Masson, Tomas","first_name":"Tomas","id":"93ac43e8-8599-11eb-9b86-f6efb0a4c207","last_name":"Masson","orcid":"0000-0002-2634-6283"},{"last_name":"Vega Zuniga","id":"2E7C4E78-F248-11E8-B48F-1D18A9856A87","full_name":"Vega Zuniga, Tomas A","first_name":"Tomas A"},{"first_name":"Ximena","full_name":"Contreras, Ximena","last_name":"Contreras","id":"475990FE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Rülicke","full_name":"Rülicke, Thomas","first_name":"Thomas"},{"first_name":"Ryuichi","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"id":"3E57A680-F248-11E8-B48F-1D18A9856A87","last_name":"Novarino","orcid":"0000-0002-7673-7178","full_name":"Novarino, Gaia","first_name":"Gaia"},{"orcid":"0000-0002-3937-1330","id":"2BD278E6-F248-11E8-B48F-1D18A9856A87","last_name":"Jösch","first_name":"Maximilian A","full_name":"Jösch, Maximilian A"}],"abstract":[{"text":"Relevant information about the data can be found in the 'Readme_Data.txt' file. \r\nA previous version of the publication can be found on BioRxiv: https://www.biorxiv.org/content/10.1101/2022.10.11.511691v4\r\nand published in Plos Biology (2024)","lang":"eng"}],"title":"Shared behavioural impairments in visual perception and place avoidance across different autism models are driven by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice","acknowledgement":"We thank Armel Nicolas, Bella Bruszel and Ewelina Dutkiewicz from the ISTA Mass Spectrometry Service (Lab Services Facilities) for all Proteomics work, including samples preparation, LC/MS data acquisition, searches and data evaluation. We thank Prof. Peter Jonas for his suggestion on the involvement of potassium channels and members of the Neuroethology group for their comments on the manuscript. Katalin Szigeti and Julie Murmann for experimental help. This research was supported by the Scientific Service Units of ISTA through resources provided by the Lab Support Facility, the Imaging and Optics Facility, the Machine Shop Unit and the Preclinical Facility, especially Freyja Langer and Michael Schunn. "},{"abstract":[{"text":"In recent years, solution processes have gained considerable traction as a cost-effective and scalable method to produce high-performance thermoelectric materials. The process entails a series of critical steps: synthesis, purification, thermal treatments, and consolidation, each playing a pivotal role in determining performance, stability, and reproducibility. We have noticed a need for more comprehensive details for each of the described steps in most published works. Recognizing the significance of detailed synthetic protocols, we describe here the approach used to synthesize and characterize one of the highest-performing polycrystalline p-type SnSe. In particular, we report the synthesis of SnSe particles in water and the subsequent surface treatment with CdSe molecular complexes that yields CdSe-SnSe nanocomposites upon consolidation. Moreover, the surface treatment inhibits grain growth through Zenner pinning of secondary phase CdSe nanoparticles and enhances defect formation at different length scales. The enhanced complexity in the CdSe-SnSe nanocomposite microstructure with respect to SnSe promotes phonon scattering and thereby significantly reduces the thermal conductivity. Such surface engineering provides opportunities in solution processing for introducing and controlling defects, making it possible to optimize the transport properties and attain a high thermoelectric figure of merit.","lang":"eng"}],"APC_amount":"4394,84 EUR","title":"Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity","publication_status":"published","acknowledgement":"The Scientific Service Units (SSU) of ISTA supported this research through resources provided by the Electron Microscopy Facility (EMF) and the Lab Support Facility (LSF). This work was financially supported by the Institute of Science and Technology Austria and the Werner Siemens Foundation.","oa":1,"OA_place":"publisher","OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)","issue":"207","author":[{"id":"bd3fceba-dc74-11ea-a0a7-c17f71817366","last_name":"Fiedler","first_name":"Christine","full_name":"Fiedler, Christine"},{"first_name":"Yu","full_name":"Liu, Yu","last_name":"Liu","id":"2A70014E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7313-6740"},{"first_name":"Maria","full_name":"Ibáñez, Maria","orcid":"0000-0001-5013-2843","id":"43C61214-F248-11E8-B48F-1D18A9856A87","last_name":"Ibáñez"}],"day":"01","ddc":["530"],"pmid":1,"external_id":{"isi":["001281657200005"],"pmid":["38829127"]},"language":[{"iso":"eng"}],"year":"2024","article_type":"original","publication":"Journal of Visualized Experiments","publication_identifier":{"issn":["1940-087X"]},"file":[{"file_name":"2024_JoVE_Fiedler.pdf","relation":"main_file","file_id":"19047","checksum":"ddb41f1ce2333484ab5cd109ac2941c0","success":1,"creator":"dernst","file_size":1371995,"date_created":"2025-02-17T15:08:55Z","date_updated":"2025-02-17T15:08:55Z","access_level":"open_access","content_type":"application/pdf"}],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"}],"isi":1,"_id":"17124","oa_version":"Published Version","month":"05","date_created":"2024-06-09T22:01:02Z","date_updated":"2025-09-08T07:51:46Z","quality_controlled":"1","volume":2024,"scopus_import":"1","project":[{"name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery","_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A"}],"doi":"10.3791/66278","has_accepted_license":"1","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (3.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/3.0/legalcode"},"type":"journal_article","date_published":"2024-05-01T00:00:00Z","citation":{"apa":"Fiedler, C., Liu, Y., &#38; Ibáñez, M. (2024). Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity. <i>Journal of Visualized Experiments</i>. MyJove Corporation. <a href=\"https://doi.org/10.3791/66278\">https://doi.org/10.3791/66278</a>","ieee":"C. Fiedler, Y. Liu, and M. Ibáñez, “Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity,” <i>Journal of Visualized Experiments</i>, vol. 2024, no. 207. MyJove Corporation, 2024.","ama":"Fiedler C, Liu Y, Ibáñez M. Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity. <i>Journal of Visualized Experiments</i>. 2024;2024(207). doi:<a href=\"https://doi.org/10.3791/66278\">10.3791/66278</a>","mla":"Fiedler, Christine, et al. “Solution-Processed, Surface-Engineered, Polycrystalline CdSe-SnSe Exhibiting Low Thermal Conductivity.” <i>Journal of Visualized Experiments</i>, vol. 2024, no. 207, e66278, MyJove Corporation, 2024, doi:<a href=\"https://doi.org/10.3791/66278\">10.3791/66278</a>.","short":"C. Fiedler, Y. Liu, M. Ibáñez, Journal of Visualized Experiments 2024 (2024).","chicago":"Fiedler, Christine, Yu Liu, and Maria Ibáñez. “Solution-Processed, Surface-Engineered, Polycrystalline CdSe-SnSe Exhibiting Low Thermal Conductivity.” <i>Journal of Visualized Experiments</i>. MyJove Corporation, 2024. <a href=\"https://doi.org/10.3791/66278\">https://doi.org/10.3791/66278</a>.","ista":"Fiedler C, Liu Y, Ibáñez M. 2024. Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity. Journal of Visualized Experiments. 2024(207), e66278."},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","intvolume":"      2024","corr_author":"1","status":"public","article_number":"e66278","file_date_updated":"2025-02-17T15:08:55Z","license":"https://creativecommons.org/licenses/by-nc-nd/3.0/","department":[{"_id":"MaIb"}],"publisher":"MyJove Corporation"}]