[{"related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"20982"}]},"language":[{"iso":"eng"}],"day":"14","degree_awarded":"MS","corr_author":"1","publisher":"Institute of Science and Technology Austria","_id":"20964","oa_version":"Published Version","article_processing_charge":"No","alternative_title":["ISTA Master’s Thesis"],"has_accepted_license":"1","date_created":"2026-01-09T09:22:48Z","ddc":["570"],"OA_place":"publisher","supervisor":[{"full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"file":[{"relation":"main_file","embargo":"2027-01-01","file_name":"2026_Vladimirtsev_Dmitrii_Thesis.pdf","date_created":"2026-01-21T14:12:13Z","file_id":"21033","date_updated":"2026-01-21T14:12:13Z","content_type":"application/pdf","file_size":2867531,"access_level":"closed","embargo_to":"open_access","checksum":"812857b2fbe3f6113bef22fd04bccd3e","creator":"dvladimi"},{"file_size":25023066,"access_level":"closed","checksum":"2b969f97f8d7461bea3d255f48c2219c","creator":"dvladimi","relation":"source_file","file_name":"Source Files.zip","date_created":"2026-01-21T14:41:58Z","file_id":"21034","content_type":"application/x-zip-compressed","date_updated":"2026-01-28T12:38:19Z"}],"author":[{"first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-04-07T11:41:44Z","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"file_date_updated":"2026-01-28T12:38:19Z","page":"22","year":"2026","publication_identifier":{"issn":["2791-4585"]},"type":"dissertation","month":"01","status":"public","citation":{"mla":"Vladimirtsev, Dmitrii. <i>Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels</i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20964\">10.15479/AT-ISTA-20964</a>.","short":"D. Vladimirtsev, Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels, Institute of Science and Technology Austria, 2026.","ista":"Vladimirtsev D. 2026. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. Institute of Science and Technology Austria.","apa":"Vladimirtsev, D. (2026). <i>Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20964\">https://doi.org/10.15479/AT-ISTA-20964</a>","ama":"Vladimirtsev D. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20964\">10.15479/AT-ISTA-20964</a>","ieee":"D. Vladimirtsev, “Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels,” Institute of Science and Technology Austria, 2026.","chicago":"Vladimirtsev, Dmitrii. “Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels.” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-20964\">https://doi.org/10.15479/AT-ISTA-20964</a>."},"date_published":"2026-01-14T00:00:00Z","title":"Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels","publication_status":"published","doi":"10.15479/AT-ISTA-20964","fulldoi":"https://doi.org/10.15479/AT-ISTA-20964","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"}]},{"ec_funded":1,"author":[{"full_name":"Mishra, Nikhil","first_name":"Nikhil","orcid":"0000-0002-6425-5788","last_name":"Mishra","id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E"},{"last_name":"Li","id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","full_name":"Li, Yuting I","first_name":"Yuting I"},{"orcid":"0000-0001-6005-1561","first_name":"Edouard B","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_type":"original","OA_place":"publisher","ddc":["570"],"file":[{"file_name":"2026_NaturePhysics_Mishra.pdf","date_created":"2026-01-21T08:21:11Z","relation":"main_file","content_type":"application/pdf","date_updated":"2026-01-21T08:21:11Z","success":1,"file_id":"21026","access_level":"open_access","file_size":7335694,"creator":"dernst","checksum":"0ab7ac2fbcb61a364dba57152db64ed7"}],"date_created":"2026-01-20T10:12:19Z","PlanS_conform":"1","oaworkid":1,"quality_controlled":"1","has_accepted_license":"1","oa":1,"article_processing_charge":"Yes (via OA deal)","_id":"21015","publisher":"Springer Nature","oa_version":"Published Version","publication":"Nature Physics","corr_author":"1","day":"05","related_material":{"link":[{"url":"https://ista.ac.at/en/news/geometry-shapes-life/","relation":"research_data","description":"News on ISTA website"}]},"language":[{"iso":"eng"}],"project":[{"name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","call_identifier":"H2020","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"call_identifier":"H2020","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413","name":"IST-BRIDGE: International postdoctoral program"},{"_id":"917c023a-16d5-11f0-9cad-eb5cafc52090","name":"Cytoplasmic self-organization into cell-like compartments as a common guiding principle in early animal development"}],"acknowledgement":"We thank N. Petridou (EMBL) for sharing results before publication. N.M. was supported by funding from the European Union’s Horizon 2020 programme under the Marie Skłodowska-Curie COFUND Actions ISTplus grant agreement number 754411. Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement number 101034413. The research was supported by funding to C.-P.H. from the NOMIS Foundation, Project ID 1.844. We would like to thank past and present members of the Heisenberg and Hannezo groups for discussions, particularly S. Shamipour, V. Doddihal, M. Jovic, N. Hino, F. N. Arslan, R. Kobylinska and C. Camelo for feedback on the draft manuscript. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria through resources provided by the Aquatics Facility, Imaging & Optics Facility (IOF), Scientific Computing (SciComp) facility and Lab Support Facility (LSF). Open access funding provided by Institute of Science and Technology (IST Austria).","department":[{"_id":"EdHa"},{"_id":"CaHe"}],"doi":"10.1038/s41567-025-03122-1","scopus_import":"1","fulldoi":"https://doi.org/10.1038/s41567-025-03122-1","abstract":[{"lang":"eng","text":"Early embryo geometry is one of the most invariant species-specific traits, yet its role in ensuring developmental reproducibility and robustness remains underexplored. Here we show that in zebrafish, the geometry of the fertilized egg—specifically its curvature and volume—serves as a critical initial condition triggering a cascade of events that influence development. The embryo geometry guides patterned asymmetric cell divisions in the blastoderm, generating radial gradients of cell volume and nucleocytoplasmic ratio. These gradients generate mitotic phase waves, with the nucleocytoplasmic ratio determining individual cell cycle periods independently of other cells. We demonstrate that reducing cell autonomy reshapes these waves, emphasizing the instructive role of geometry-derived volume patterns in setting the intrinsic period of the cell cycle oscillator. In addition to organizing cell cycles, early embryo geometry spatially patterns zygotic genome activation at the midblastula transition, a key step in establishing embryonic autonomy. Disrupting the embryo shape alters the zygotic genome activation pattern and causes ectopic germ layer specification, underscoring the developmental significance of geometry. Together, our findings reveal a symmetry-breaking function of early embryo geometry in coordinating cell cycle and transcriptional patterning."}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"external_id":{"oaworkid":["W7118187193"]},"publication_status":"published","citation":{"chicago":"Mishra, Nikhil, Yuting I Li, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>.","ieee":"N. Mishra, Y. I. Li, E. B. Hannezo, and C.-P. J. Heisenberg, “Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo,” <i>Nature Physics</i>, vol. 22. Springer Nature, pp. 139–150, 2026.","short":"N. Mishra, Y.I. Li, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 22 (2026) 139–150.","mla":"Mishra, Nikhil, et al. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>, vol. 22, Springer Nature, 2026, pp. 139–50, doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>.","ista":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. 2026. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. Nature Physics. 22, 139–150.","apa":"Mishra, N., Li, Y. I., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2026). Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>","ama":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. 2026;22:139-150. doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>"},"intvolume":"        22","title":"Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo","date_published":"2026-01-05T00:00:00Z","status":"public","month":"01","type":"journal_article","OA_type":"hybrid","year":"2026","page":"139-150","license":"https://creativecommons.org/licenses/by/4.0/","volume":22,"publication_identifier":{"issn":["1745-2473"],"issnl":[" 1745-2473"],"eissn":["1745-2481"]},"date_updated":"2026-04-28T12:55:30Z","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"file_date_updated":"2026-01-21T08:21:11Z"},{"corr_author":"1","_id":"21040","oa_version":"Published Version","publisher":"American Chemical Society","article_processing_charge":"Yes (via OA deal)","publication":"ACS Applied Energy Materials","language":[{"iso":"eng"}],"day":"12","ddc":["540"],"OA_place":"publisher","article_type":"original","file":[{"access_level":"open_access","file_size":5977526,"creator":"dernst","checksum":"81272c19df41c696c1737168d3ea8c16","date_created":"2026-02-12T13:55:28Z","file_name":"2026_AppliedEnergyMaterials_Busato.pdf","relation":"main_file","success":1,"content_type":"application/pdf","date_updated":"2026-02-12T13:55:28Z","file_id":"21222"}],"author":[{"full_name":"Busato, Matteo","first_name":"Matteo","last_name":"Busato"},{"first_name":"Mariarosaria","full_name":"Tuccillo, Mariarosaria","last_name":"Tuccillo"},{"last_name":"Celeste","first_name":"Arcangelo","full_name":"Celeste, Arcangelo"},{"first_name":"Alessandro","full_name":"Tofoni, Alessandro","last_name":"Tofoni"},{"full_name":"Silvestri, Laura","first_name":"Laura","last_name":"Silvestri"},{"last_name":"D’Angelo","full_name":"D’Angelo, Paola","first_name":"Paola"},{"orcid":"0000-0003-2902-5319","first_name":"Stefan Alexander","full_name":"Freunberger, Stefan Alexander","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425","last_name":"Freunberger"},{"last_name":"Brutti","first_name":"Sergio","full_name":"Brutti, Sergio"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","oa":1,"has_accepted_license":"1","PlanS_conform":"1","date_created":"2026-01-25T23:01:40Z","month":"01","type":"journal_article","status":"public","OA_type":"hybrid","intvolume":"         9","citation":{"chicago":"Busato, Matteo, Mariarosaria Tuccillo, Arcangelo Celeste, Alessandro Tofoni, Laura Silvestri, Paola D’Angelo, Stefan Alexander Freunberger, and Sergio Brutti. “Structural Rearrangements of a Cobalt-Free Lithium-Rich Layered Oxide Cathode during Formation.” <i>ACS Applied Energy Materials</i>. American Chemical Society, 2026. <a href=\"https://doi.org/10.1021/acsaem.5c03511\">https://doi.org/10.1021/acsaem.5c03511</a>.","ieee":"M. Busato <i>et al.</i>, “Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation,” <i>ACS Applied Energy Materials</i>, vol. 9, no. 1. American Chemical Society, pp. 686–697, 2026.","short":"M. Busato, M. Tuccillo, A. Celeste, A. Tofoni, L. Silvestri, P. D’Angelo, S.A. Freunberger, S. Brutti, ACS Applied Energy Materials 9 (2026) 686–697.","mla":"Busato, Matteo, et al. “Structural Rearrangements of a Cobalt-Free Lithium-Rich Layered Oxide Cathode during Formation.” <i>ACS Applied Energy Materials</i>, vol. 9, no. 1, American Chemical Society, 2026, pp. 686–97, doi:<a href=\"https://doi.org/10.1021/acsaem.5c03511\">10.1021/acsaem.5c03511</a>.","ista":"Busato M, Tuccillo M, Celeste A, Tofoni A, Silvestri L, D’Angelo P, Freunberger SA, Brutti S. 2026. Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation. ACS Applied Energy Materials. 9(1), 686–697.","apa":"Busato, M., Tuccillo, M., Celeste, A., Tofoni, A., Silvestri, L., D’Angelo, P., … Brutti, S. (2026). Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation. <i>ACS Applied Energy Materials</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acsaem.5c03511\">https://doi.org/10.1021/acsaem.5c03511</a>","ama":"Busato M, Tuccillo M, Celeste A, et al. Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation. <i>ACS Applied Energy Materials</i>. 2026;9(1):686-697. doi:<a href=\"https://doi.org/10.1021/acsaem.5c03511\">10.1021/acsaem.5c03511</a>"},"date_published":"2026-01-12T00:00:00Z","title":"Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode during formation","date_updated":"2026-02-12T14:04:04Z","file_date_updated":"2026-02-12T13:55:28Z","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"LifeSc"}],"page":"686-697","year":"2026","issue":"1","publication_identifier":{"eissn":["2574-0962"]},"volume":9,"department":[{"_id":"StFr"}],"acknowledgement":"Elettra-Sincrotrone Trieste S.C.p.A. and its staff are acknowledged for providing synchrotron radiation beamtime and laboratory facilities, in particular the MCX and XAFS beamlines, where the XRD and XAS experiments have been carried out, supported by the projects number: 20217082, 20205109, and 20195014. This study was carried out within the MOST─Sustainable Mobility Center and received funding from the European Union Next-Generation EU (PIANO NAZIONALE DI RIPRESA E RESILIENZA (PNRR)─MISSIONE 4 COMPONENTE 2, INVESTIMENTO 1.4─D.D. 1033 17/06/2022, CN00000023). Moreover, the contribution of S.B. and A.C. to this study was carried out within the NEST─Network for Energy Sustainable Transition and received funding from the European Union Next-Generation EU (PNRR─MISSIONE 4 COMPONENTE 2, INVESTIMENTO 1.3─D.D. 1561 11/10/2022, B53C22004070006). This manuscript reflects only the authors’ views and opinions, neither the European Union nor the European Commission can be considered responsible for them. Two of us, S.B. and S.A.F., would like to thank the Alistore ERI. L.S. received funds from the Ministry of Ecological Transition in the “Ricerca di Sistema Elettrico” framework. S.A.F. is indebted to ISTA for support. The Scientific Service Units of ISTA supported this research through resources provided by the Lab Support Facility and the Miba Machine Shop.","publication_status":"published","doi":"10.1021/acsaem.5c03511","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"abstract":[{"text":"Formation during the first cycles of Li-rich layered oxide (LRLO) cathode materials consolidates the interphase and leads to structural changes that are decisive for long-term cyclability. However, the nature and effect of the changes are material-dependent and unknown for the important class of Co-free, Ni-poor LRLOs. Here, we analyze the processes during the tailored formation procedure of a typical class member, Li1.28Ni0.15Mn0.57O2, and demonstrate that it remarkably changes lattice composition and structure as a prerequisite for stable cycling. We combine electrochemistry, operando mass spectrometry, X-ray diffraction, and X-ray absorption spectroscopy with density functional theory simulations. Activation most prominently compresses the layer spacing along the c-axis and increases reversible structural breathing. The large capacity of ∼250 mAh g–1 originates from the Ni2+/Ni4+ and O2–/O– redox couples. Electron exchange during O-redox is smeared over the entire anionic sublattice rather than localized on specific oxygen atomic sites. This redox mechanism is reversible without detrimental oxygen evolution, avoiding continued degradation common in conventional LRLOs. Sequential Ni- and O-redox during activation irreversibly distorts the coordination of the redox-inactive Mn4+ centers. This structural evolution of the MnO6 octahedra appears to enable the superior electrochemical performance of this LRLO phase. These findings define an activation pathway for the important class of Co-free, Ni-poor LRLOs, offering potential guidance for the rational design of high-performance, more sustainable cathode materials.","lang":"eng"}],"fulldoi":"https://doi.org/10.1021/acsaem.5c03511","scopus_import":"1"},{"tmp":{"short":"CC BY-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","image":"/images/cc_by_sa.png"},"fulldoi":"https://doi.org/10.15479/AT-ISTA-21137","doi":"10.15479/AT-ISTA-21137","department":[{"_id":"GradSch"},{"_id":"CaHe"},{"_id":"EdHa"}],"project":[{"name":"International IST Doctoral Program","grant_number":"665385","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"name":"Keratins in epithelial tissue spreading","grant_number":"PAT 5044023","_id":"8f060199-16d5-11f0-9cad-f3253b266c46"},{"_id":"252C3B08-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"W1250-B20","name":"Nano-Analytics of Cellular Systems"}],"acknowledgement":"We thank all members of the Heisenberg, Henkes, and Hannezo groups for their support. We are also grateful to the Imaging and Optics, Scientific Computing, Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their technical assistance and support. Numerical simulations were performed using the computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020 research and innovation programme (grant agreement No. 665385). This work was supported by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to C.-P.H.","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"file_date_updated":"2026-03-24T07:21:43Z","date_updated":"2026-06-10T09:44:10Z","license":"https://creativecommons.org/licenses/by-sa/4.0/","year":"2026","month":"3","type":"research_data","status":"public","date_published":"2026-03-24T00:00:00Z","title":"Data associated with Keratins coordinate tissue spreading ","citation":{"ista":"Naik S. 2026. Data associated with Keratins coordinate tissue spreading , Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>.","ama":"Naik S. Data associated with Keratins coordinate tissue spreading . 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>","apa":"Naik, S. (2026). Data associated with Keratins coordinate tissue spreading . Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">https://doi.org/10.15479/AT-ISTA-21137</a>","short":"S. Naik, (2026).","mla":"Naik, Suyash. <i>Data Associated with Keratins Coordinate Tissue Spreading </i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21137\">10.15479/AT-ISTA-21137</a>.","chicago":"Naik, Suyash. “Data Associated with Keratins Coordinate Tissue Spreading .” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-21137\">https://doi.org/10.15479/AT-ISTA-21137</a>.","ieee":"S. Naik, “Data associated with Keratins coordinate tissue spreading .” Institute of Science and Technology Austria, 2026."},"oa":1,"has_accepted_license":"1","date_created":"2026-02-04T16:38:02Z","contributor":[{"last_name":"Keta","contributor_type":"researcher","first_name":"Yann-Edwin"},{"last_name":"Henkes","contributor_type":"supervisor","first_name":"Silke "},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","contributor_type":"supervisor","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","contributor_type":"supervisor","orcid":"0000-0001-6005-1561","first_name":"Edouard B"}],"file":[{"date_created":"2026-03-16T11:51:10Z","file_name":"cells-main.zip","description":"Python3 library written in C++20 to integrate vertex models. Please read the readme at https://github.com/yketa/cells/blob/main/README.md for detailed instructions for installation and usage of the code in this repository. 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Aside from solution NMR, an increasing number of studies have been conducted applying solid-state magic-angle-spinning NMR to fluorine-labeled samples. Here, the high chemical shift anisotropy and strong dipolar couplings can be utilized to get structural insights into proteins and measure long distances. Despite increasing popularity and promising benefits, the sensitivity of biomolecular 19F MAS NMR often suffers from slow longitudinal T1 relaxation and therefore long recycle delays. In this work, we expand paramagnetic doping, an approach commonly used to reduce proton T1 relaxation times, to 19F-labeled biological samples. We study the effect of Gd(DTPA) and Gd(DTPA-BMA) on 19F and 13C T1 and T2 relaxation in a [5-19F13C]-tryptophan-labeled protein via 19F-detected MAS NMR experiments. The observed paramagnetic relaxation enhancement substantially reduces measurement times of 19F MAS NMR experiments without compromising resolution. Additionally, we report the chemical-shift assignments of all four fluorotryptophan signals in the 12 × 39 kDa large protein using a mutagenesis approach.","lang":"eng"}],"fulldoi":"https://doi.org/10.15479/AT-ISTA-21284","department":[{"_id":"GradSch"},{"_id":"PaSc"}],"acknowledgement":"We thank Ben P. Tatman for insightful discussions. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance Facility and the Lab Support Facility.","date_updated":"2026-06-10T09:28:41Z","acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"file_date_updated":"2026-02-17T10:11:14Z","license":"https://creativecommons.org/licenses/by-nc/4.0/","year":"2026","month":"2","type":"research_data","status":"public","OA_type":"free access","citation":{"chicago":"Becker, Lea Marie, and Paul Schanda. “Research Data for ‘Accelerated 19F Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.’” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-21284\">https://doi.org/10.15479/AT-ISTA-21284</a>.","ieee":"L. M. Becker and P. Schanda, “Research data for ‘Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants.’” Institute of Science and Technology Austria, 2026.","short":"L.M. Becker, P. Schanda, (2026).","mla":"Becker, Lea Marie, and Paul Schanda. <i>Research Data for “Accelerated 19F Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.”</i> Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21284\">10.15479/AT-ISTA-21284</a>.","ama":"Becker LM, Schanda P. Research data for “Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants.” 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21284\">10.15479/AT-ISTA-21284</a>","ista":"Becker LM, Schanda P. 2026. Research data for ‘Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT-ISTA-21284\">10.15479/AT-ISTA-21284</a>.","apa":"Becker, L. M., &#38; Schanda, P. (2026). Research data for “Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-21284\">https://doi.org/10.15479/AT-ISTA-21284</a>"},"date_published":"2026-02-18T00:00:00Z","title":"Research data for \"Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants\""},{"OA_type":"hybrid","status":"public","type":"journal_article","month":"03","title":"Adventitious carbon breaks symmetry in oxide contact electrification","date_published":"2026-03-18T00:00:00Z","citation":{"ieee":"G. M. Grosjean <i>et al.</i>, “Adventitious carbon breaks symmetry in oxide contact electrification,” <i>Nature</i>, vol. 651, no. 8106. Springer Nature, pp. 626–631, 2026.","chicago":"Grosjean, Galien M, Markus Ostermann, Markus Sauer, Michael Hahn, Christian M. Pichler, Florian Fahrnberger, Felix Pertl, et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” <i>Nature</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41586-025-10088-w\">https://doi.org/10.1038/s41586-025-10088-w</a>.","mla":"Grosjean, Galien M., et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” <i>Nature</i>, vol. 651, no. 8106, Springer Nature, 2026, pp. 626–31, doi:<a href=\"https://doi.org/10.1038/s41586-025-10088-w\">10.1038/s41586-025-10088-w</a>.","short":"G.M. Grosjean, M. Ostermann, M. Sauer, M. Hahn, C.M. Pichler, F. Fahrnberger, F. Pertl, D. Balazs, M.M. Link, S.H. Kim, D.L. Schrader, A. Blanco, F. Gracia, N. Mujica, S.R. Waitukaitis, Nature 651 (2026) 626–631.","ista":"Grosjean GM, Ostermann M, Sauer M, Hahn M, Pichler CM, Fahrnberger F, Pertl F, Balazs D, Link MM, Kim SH, Schrader DL, Blanco A, Gracia F, Mujica N, Waitukaitis SR. 2026. Adventitious carbon breaks symmetry in oxide contact electrification. Nature. 651(8106), 626–631.","apa":"Grosjean, G. M., Ostermann, M., Sauer, M., Hahn, M., Pichler, C. M., Fahrnberger, F., … Waitukaitis, S. R. (2026). Adventitious carbon breaks symmetry in oxide contact electrification. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-10088-w\">https://doi.org/10.1038/s41586-025-10088-w</a>","ama":"Grosjean GM, Ostermann M, Sauer M, et al. Adventitious carbon breaks symmetry in oxide contact electrification. <i>Nature</i>. 2026;651(8106):626-631. doi:<a href=\"https://doi.org/10.1038/s41586-025-10088-w\">10.1038/s41586-025-10088-w</a>"},"intvolume":"       651","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"file_date_updated":"2026-03-24T06:57:08Z","date_updated":"2026-04-28T12:06:01Z","volume":651,"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"year":"2026","issue":"8106","page":"626-631","department":[{"_id":"ScWa"},{"_id":"GradSch"},{"_id":"LifeSc"}],"project":[{"name":"Tribocharge: a multi-scale approach to an enduring problem in physics","grant_number":"949120","_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","call_identifier":"H2020"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships"}],"acknowledgement":"This project has received support from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 949120) and from the Marie Skłodowska-Curie programme (grant agreement no. 754411). We acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. N.M. acknowledges support from grant Fondecyt 1221597. G.G. is a Serra Húnter fellow. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility and Lab Support Facility. We thank the Modic group for the use of the Laue camera, T. Zauner for the photography of the experimental set-up and R. Möller for insightful discussions. Open access funding provided by Institute of Science and Technology (IST Austria).","external_id":{"pmid":["41851325"]},"publication_status":"published","fulldoi":"https://doi.org/10.1038/s41586-025-10088-w","abstract":[{"lang":"eng","text":"Insulating oxides are among the most abundant solid materials in the universe1,2,3. Of the many ways in which they influence natural phenomena, perhaps the most consequential is their capacity to transfer electrical charge during contact4,5,6,7,8,9,10—which occurs even between samples of the same oxide—yet the symmetry-breaking parameter that causes this remains unidentified11,12. Here we show that adventitious carbonaceous molecules adsorbed from the environment are the symmetry-breaking factor in same-material oxide contact electrification (CE). We use acoustic levitation to measure charge exchange between a sphere and a plate composed of identical amorphous silicon dioxide (SiO2). Although charging polarity is random for co-prepared samples, we control it with baking or plasma treatment. Observing the charge-exchange relaxation afterwards, we see dynamics over a timescale of hours and connect this directly to the presence of adventitious carbon with time-of-flight mass spectrometry, low-energy ion scattering and infrared spectroscopy. Going further, we confirm that adventitious carbon can even determine charge exchange among different oxides. Our results identify the symmetry-breaking parameter that causes insulating oxides to exchange charge in settings ranging from desert sands4 to volcanic plumes5,6, while simultaneously highlighting an overlooked factor in CE more broadly."}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"doi":"10.1038/s41586-025-10088-w","corr_author":"1","publication":"Nature","article_processing_charge":"Yes (via OA deal)","oa_version":"Published Version","_id":"21485","publisher":"Springer Nature","language":[{"iso":"eng"}],"related_material":{"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/colliding-dust-and-the-sparks-of-creation/"}]},"day":"18","file":[{"file_id":"21494","success":1,"date_updated":"2026-03-24T06:57:08Z","content_type":"application/pdf","relation":"main_file","date_created":"2026-03-24T06:57:08Z","file_name":"2026_Nature_Grosjean.pdf","checksum":"dafef9ed575b44be4263e948a47ae056","creator":"dernst","file_size":12245694,"access_level":"open_access"}],"article_type":"original","OA_place":"publisher","ddc":["540"],"pmid":1,"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","ec_funded":1,"author":[{"last_name":"Grosjean","id":"0C5FDA4A-9CF6-11E9-8939-FF05E6697425","full_name":"Grosjean, Galien M","first_name":"Galien M","orcid":"0000-0001-5154-417X"},{"full_name":"Ostermann, Markus","first_name":"Markus","last_name":"Ostermann"},{"first_name":"Markus","full_name":"Sauer, Markus","last_name":"Sauer"},{"first_name":"Michael","full_name":"Hahn, Michael","last_name":"Hahn"},{"last_name":"Pichler","full_name":"Pichler, Christian M.","first_name":"Christian M."},{"last_name":"Fahrnberger","full_name":"Fahrnberger, Florian","first_name":"Florian"},{"full_name":"Pertl, Felix","first_name":"Felix","orcid":"0000-0003-0463-5794","last_name":"Pertl","id":"6313aec0-15b2-11ec-abd3-ed67d16139af"},{"last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","full_name":"Balazs, Daniel","orcid":"0000-0001-7597-043X","first_name":"Daniel"},{"full_name":"Link, Mason M.","first_name":"Mason M.","last_name":"Link"},{"full_name":"Kim, Seong H.","first_name":"Seong H.","last_name":"Kim"},{"full_name":"Schrader, Devin L.","first_name":"Devin L.","last_name":"Schrader"},{"full_name":"Blanco, Adriana","first_name":"Adriana","last_name":"Blanco"},{"first_name":"Francisco","full_name":"Gracia, Francisco","last_name":"Gracia"},{"full_name":"Mujica, Nicolás","first_name":"Nicolás","last_name":"Mujica"},{"full_name":"Waitukaitis, Scott R","orcid":"0000-0002-2299-3176","first_name":"Scott R","last_name":"Waitukaitis","id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87"}],"has_accepted_license":"1","oa":1,"quality_controlled":"1","date_created":"2026-03-23T15:04:00Z","PlanS_conform":"1"},{"day":"23","language":[{"iso":"eng"}],"publication":"Current Biology","publisher":"Elsevier","_id":"21490","oa_version":"Published Version","article_processing_charge":"Yes (via OA deal)","corr_author":"1","PlanS_conform":"1","date_created":"2026-03-23T15:11:16Z","oa":1,"has_accepted_license":"1","quality_controlled":"1","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"id":"01f96916-0235-11eb-9379-a323192643b7","last_name":"Li","first_name":"Mingyue","full_name":"Li, Mingyue"},{"last_name":"Rydza","first_name":"Nikola","full_name":"Rydza, Nikola"},{"full_name":"Mazur, Ewa","first_name":"Ewa","last_name":"Mazur"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","last_name":"Molnar","first_name":"Gergely","full_name":"Molnar, Gergely"},{"last_name":"Nodzyński","full_name":"Nodzyński, Tomasz","first_name":"Tomasz"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"file":[{"date_created":"2026-03-24T08:34:37Z","file_name":"2026_CurrentBiology_Li.pdf","relation":"main_file","success":1,"date_updated":"2026-03-24T08:34:37Z","content_type":"application/pdf","file_id":"21496","access_level":"open_access","file_size":12986894,"creator":"dernst","checksum":"fe6c41fdab58a55df5f2a5860c02acdc"}],"ddc":["580"],"article_type":"original","OA_place":"publisher","publication_identifier":{"issn":["0960-9822"]},"volume":36,"page":"1468-1480.e6","issue":"6","year":"2026","file_date_updated":"2026-03-24T08:34:37Z","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"Bio"},{"_id":"LifeSc"}],"date_updated":"2026-03-24T08:36:40Z","date_published":"2026-03-23T00:00:00Z","title":"Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization","intvolume":"        36","citation":{"ieee":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” <i>Current Biology</i>, vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026.","chicago":"Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>.","mla":"Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>, vol. 36, no. 6, Elsevier, 2026, p. 1468–1480.e6, doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>.","short":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6.","apa":"Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., &#38; Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>","ista":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6.","ama":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. 2026;36(6):1468-1480.e6. doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>"},"OA_type":"hybrid","type":"journal_article","month":"03","status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"abstract":[{"text":"Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development.","lang":"eng"}],"fulldoi":"https://doi.org/10.1016/j.cub.2026.02.023","doi":"10.1016/j.cub.2026.02.023","publication_status":"published","external_id":{"pmid":["41831441"]},"acknowledgement":"We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.","project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"_id":"bd906599-d553-11ed-ba76-abf8547645d7","grant_number":"E271","name":"Identification of a novel regulator in auxin canalization"}],"department":[{"_id":"JiFr"}]},{"day":"10","language":[{"iso":"eng"}],"publication":"Science Advances","publisher":"AAAS","_id":"21750","oa_version":"Published Version","article_processing_charge":"Yes","article_number":"eaec9073","date_created":"2026-04-19T22:07:47Z","DOAJ_listed":"1","oa":1,"has_accepted_license":"1","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"author":[{"last_name":"Li","full_name":"Li, Mengyao","first_name":"Mengyao"},{"full_name":"Zhao, Xueke","first_name":"Xueke","last_name":"Zhao"},{"first_name":"Yu","full_name":"Zhang, Yu","last_name":"Zhang"},{"last_name":"Yu","first_name":"Jing","full_name":"Yu, Jing"},{"full_name":"Liu, Xuyang","first_name":"Xuyang","last_name":"Liu"},{"last_name":"Jia","first_name":"Mochen","full_name":"Jia, Mochen"},{"first_name":"Hongzhang","full_name":"Song, Hongzhang","last_name":"Song"},{"full_name":"Wang, Dongyang","first_name":"Dongyang","last_name":"Wang"},{"full_name":"Arbiol, Jordi","first_name":"Jordi","last_name":"Arbiol"},{"last_name":"Ibáñez","id":"43C61214-F248-11E8-B48F-1D18A9856A87","full_name":"Ibáñez, Maria","first_name":"Maria","orcid":"0000-0001-5013-2843"},{"first_name":"Chongxin","full_name":"Shan, Chongxin","last_name":"Shan"},{"first_name":"Andreu","full_name":"Cabot, Andreu","last_name":"Cabot"},{"first_name":"Ziyu","full_name":"Wang, Ziyu","last_name":"Wang"}],"file":[{"checksum":"9bd4546a23f218972f83164fb21003e1","creator":"dernst","file_size":3727993,"access_level":"open_access","file_id":"21802","success":1,"date_updated":"2026-05-06T06:06:26Z","content_type":"application/pdf","relation":"main_file","date_created":"2026-05-06T06:06:26Z","file_name":"2026_ScienceAdv_Li.pdf"}],"ddc":["530"],"OA_place":"publisher","article_type":"original","publication_identifier":{"eissn":["2375-2548"]},"volume":12,"issue":"15","year":"2026","file_date_updated":"2026-05-06T06:06:26Z","acknowledged_ssus":[{"_id":"LifeSc"}],"date_updated":"2026-05-06T06:08:27Z","date_published":"2026-04-10T00:00:00Z","title":"Electronic-phononic decoupling and Fermi-level tuning enable high thermoelectric performance in Ag8SnSe6","intvolume":"        12","citation":{"ieee":"M. Li <i>et al.</i>, “Electronic-phononic decoupling and Fermi-level tuning enable high thermoelectric performance in Ag8SnSe6,” <i>Science Advances</i>, vol. 12, no. 15. AAAS, 2026.","chicago":"Li, Mengyao, Xueke Zhao, Yu Zhang, Jing Yu, Xuyang Liu, Mochen Jia, Hongzhang Song, et al. “Electronic-Phononic Decoupling and Fermi-Level Tuning Enable High Thermoelectric Performance in Ag8SnSe6.” <i>Science Advances</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/sciadv.aec9073\">https://doi.org/10.1126/sciadv.aec9073</a>.","apa":"Li, M., Zhao, X., Zhang, Y., Yu, J., Liu, X., Jia, M., … Wang, Z. (2026). Electronic-phononic decoupling and Fermi-level tuning enable high thermoelectric performance in Ag8SnSe6. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.aec9073\">https://doi.org/10.1126/sciadv.aec9073</a>","ama":"Li M, Zhao X, Zhang Y, et al. Electronic-phononic decoupling and Fermi-level tuning enable high thermoelectric performance in Ag8SnSe6. <i>Science Advances</i>. 2026;12(15). doi:<a href=\"https://doi.org/10.1126/sciadv.aec9073\">10.1126/sciadv.aec9073</a>","ista":"Li M, Zhao X, Zhang Y, Yu J, Liu X, Jia M, Song H, Wang D, Arbiol J, Ibáñez M, Shan C, Cabot A, Wang Z. 2026. Electronic-phononic decoupling and Fermi-level tuning enable high thermoelectric performance in Ag8SnSe6. Science Advances. 12(15), eaec9073.","mla":"Li, Mengyao, et al. “Electronic-Phononic Decoupling and Fermi-Level Tuning Enable High Thermoelectric Performance in Ag8SnSe6.” <i>Science Advances</i>, vol. 12, no. 15, eaec9073, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aec9073\">10.1126/sciadv.aec9073</a>.","short":"M. Li, X. Zhao, Y. Zhang, J. Yu, X. Liu, M. Jia, H. Song, D. Wang, J. Arbiol, M. Ibáñez, C. Shan, A. Cabot, Z. Wang, Science Advances 12 (2026)."},"OA_type":"gold","month":"04","type":"journal_article","status":"public","abstract":[{"lang":"eng","text":"Liquid-like superionic conductors, with highly mobile ions in a rigid framework, offer intrinsically low lattice thermal conductivity without compromising electronic transport. Argyrodite-type Ag8SnSe6 exhibits a melt-like Ag sublattice that drives lattice thermal conductivity (κL) below 0.2 watts per meter per kelvin, yet its low carrier concentration limits the power factor. Here, interstitial Ag atoms raise the Fermi level into the conduction band, substantially increasing the electron concentration. Simultaneously, the formation of a secondary Ag2Se phase generates lattice distortions that enhance phonon scattering. A pronounced mismatch between electronic (~200 nanometers) and phononic (~0.22 nanometers) mean free paths decouples charge and heat transport, enabling concurrent suppression of κL and retention of high electrical conductivity. This coupled electronic-phononic modulation yields a record ZT of 0.72 at ambient temperature and a peak ZT of 1.1 at 735 kelvins, with an average ZTavg of 0.72 over 320 to 735 kelvins. A unicouple device achieves 6.3% efficiency under a 357-kelvin gradient, highlighting a practical strategy for high-performance midtemperature thermoelectrics."}],"tmp":{"short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"fulldoi":"https://doi.org/10.1126/sciadv.aec9073","scopus_import":"1","doi":"10.1126/sciadv.aec9073","publication_status":"published","external_id":{"pmid":["41961944"]},"acknowledgement":"The Scientific Service Units (SSU) of ISTA supported this research through resources provided by the Lab Support Facility (LSF). This work was supported by the National Key R&D Program of China grant 2024YFE0105200 (to C.S.), National Natural Science Foundation of China grant 12504038 (to M.L.), China Postdoctoral Science Foundation grant 2023M743151 (to M.L.), Natural Science Foundation of Henan Province grant 252300421763 (to M.L.), Key Scientific Research Project of Higher Education Institutions in Henan Province grant 25A140004 (to M.L.), National Natural Science Foundation of China grant 12204156 (to D.W.), China Postdoctoral Science Foundation grant 2023TQ0315 and 2023 M743224 (to D.W.), Generalitat de Catalunya grant 2021SGR00457 (to J.A.), and European Regional Development Fund grants ENE2016-77798-C4-3-R, PID2020-116093RB-C43, and AEI/10.13039/501100011033 (to A.C.). This work also was financially supported by ISTA and the Werner Siemens Foundation (to M.I.).","project":[{"name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery","_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A"}],"department":[{"_id":"MaIb"}]},{"oa":1,"has_accepted_license":"1","quality_controlled":"1","date_created":"2026-04-26T22:01:47Z","file":[{"file_id":"21832","success":1,"date_updated":"2026-05-07T05:54:43Z","content_type":"application/pdf","relation":"main_file","date_created":"2026-05-07T05:54:43Z","file_name":"2026_Science_Kulich_accepted.pdf","checksum":"eb5b29247832ecdc53c8146da0509bbe","creator":"dernst","file_size":6150733,"access_level":"open_access"}],"ddc":["580"],"article_type":"original","OA_place":"repository","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"author":[{"first_name":"Ivan","full_name":"Kulich, Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich"},{"full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii","last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d"},{"id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","first_name":"Marek","full_name":"Randuch, Marek"},{"last_name":"Gao","first_name":"Shiqiang","full_name":"Gao, Shiqiang"},{"first_name":"Matteo","full_name":"Citterico, Matteo","last_name":"Citterico"},{"full_name":"Konrad, Kai R.","first_name":"Kai R.","last_name":"Konrad"},{"first_name":"Georg","full_name":"Nagel, Georg","last_name":"Nagel"},{"last_name":"Wrzaczek","first_name":"Michael","full_name":"Wrzaczek, Michael"},{"last_name":"Cascaro","full_name":"Cascaro, Léa","first_name":"Léa"},{"last_name":"Vinet","first_name":"Pauline","full_name":"Vinet, Pauline"},{"last_name":"Durand","first_name":"Pauline","full_name":"Durand, Pauline"},{"full_name":"Asnacios, Atef","first_name":"Atef","last_name":"Asnacios"},{"first_name":"Lokesh","full_name":"Verma, Lokesh","last_name":"Verma"},{"full_name":"Bennett, Malcolm J.","first_name":"Malcolm J.","last_name":"Bennett"},{"full_name":"Pandey, Bipin K.","first_name":"Bipin K.","last_name":"Pandey"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"language":[{"iso":"eng"}],"day":"16","corr_author":"1","publication":"Science","_id":"21763","oa_version":"Accepted Version","publisher":"AAAS","article_processing_charge":"No","publication_status":"published","external_id":{"pmid":["41990180"]},"abstract":[{"lang":"eng","text":"Reactive oxygen species (ROS) have been implicated in multiple signaling processes in plants, but the underlying mechanisms and roles remain enigmatic. In this study, we developed a method of live imaging of apoplastic ROS at the root surface. Distinct signals, including auxin, extracellular adenosine triphosphate, and rapid alkalinization factor 1 peptide, induce cytosolic calcium transients and apoplastic ROS bursts. Genetic and optogenetic manipulations of Arabidopsis identified calcium transients as necessary and sufficient for ROS bursts through activation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases RBOHC and RBOHF. Apoplastic ROS bursts are not required, but they do limit gravity-induced root bending. Root bending is sensed by the stretch-activated calcium channel MCA1, leading to NADPH oxidase activation. The resulting ROS production stiffens cell walls to facilitate soil penetration. Apoplastic ROS thus provides a means to balance tissue flexibility and stiffness to navigate soil."}],"fulldoi":"https://doi.org/10.1126/science.adu8197","scopus_import":"1","doi":"10.1126/science.adu8197","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"acknowledgement":"We gratefully acknowledge the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) (both of ISTA) and the Hounsfield CT Facility (University of Nottingham) for support with imaging and the Growth Facility (IPMB) for plant cultivation. We thank M. Fendrych and his team for help with the microfluidics upgrades and J. Atkinson at the University of Nottingham MakerSpace for 3D printing of Arabidopsis mini-soil columns.\r\nThis project received funding from the European Research Council (ERC; 101142681 CYNIPS) and the Austrian Science Fund (FWF; P 37051-B). I.K. was cofunded by the European Union, Horizon Europe, project MOLIPEC, ID 101087030 and CSF project 25-16449S. L.V. and B.K.P. acknowledge funding from UK Research and Innovation (UKRI) Frontiers Research (EP/Y036697/1). M.J.B. acknowledges funding from ERC SYNERGY (grant 101118769 HYDROSENSING). The study was partially supported by the Université Paris Cité, Idex ANR-18-IDEX-0001, funded by the French Government through its “Investments for the Future” program and also by the projects “Mecha-Nuc” ANR-20-CE13-0025-03 and “scEm-bryoMech” ANR-21-CE13-0046. P.D. acknowledges support by Human Frontier Science Program Organization grant 2022-RG107. P.V. acknowledges support provided by “Programme blanc” of the Graduate School BIOSPHERA, Université Paris-Saclay. Phytohormonal analysis was performed using the service laboratory funded by Toward Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the European Regional Development Fund (ERDF) program Johannes Amos Comenius. This research was funded in whole or in part by the Austrian Science Fund (P 37051-B) and UK Research and Innovation (EP/Y036697/1), cOAlition S organizations, and by the European Research Council (101142681 CYNIPS, 101118769 HYDROSENSING); as required, the author will make the Author Accepted Manuscript (AAM) version available under a CC BY public copyright license.","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"file_date_updated":"2026-05-07T05:54:43Z","date_updated":"2026-05-07T06:20:07Z","publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"volume":392,"page":"296-300","issue":"6795","year":"2026","OA_type":"green","type":"journal_article","month":"04","status":"public","date_published":"2026-04-16T00:00:00Z","title":"Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation","intvolume":"       392","citation":{"mla":"Kulich, Ivan, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>, vol. 392, no. 6795, AAAS, 2026, pp. 296–300, doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>.","short":"I. Kulich, D. Vladimirtsev, M. Randuch, S. Gao, M. Citterico, K.R. Konrad, G. Nagel, M. Wrzaczek, L. Cascaro, P. Vinet, P. Durand, A. Asnacios, L. Verma, M.J. Bennett, B.K. Pandey, J. Friml, Science 392 (2026) 296–300.","apa":"Kulich, I., Vladimirtsev, D., Randuch, M., Gao, S., Citterico, M., Konrad, K. R., … Friml, J. (2026). Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>","ama":"Kulich I, Vladimirtsev D, Randuch M, et al. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. 2026;392(6795):296-300. doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>","ista":"Kulich I, Vladimirtsev D, Randuch M, Gao S, Citterico M, Konrad KR, Nagel G, Wrzaczek M, Cascaro L, Vinet P, Durand P, Asnacios A, Verma L, Bennett MJ, Pandey BK, Friml J. 2026. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 392(6795), 296–300.","ieee":"I. Kulich <i>et al.</i>, “Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation,” <i>Science</i>, vol. 392, no. 6795. AAAS, pp. 296–300, 2026.","chicago":"Kulich, Ivan, Dmitrii Vladimirtsev, Marek Randuch, Shiqiang Gao, Matteo Citterico, Kai R. Konrad, Georg Nagel, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>."}},{"author":[{"first_name":"Adam","full_name":"Petrik, Adam","id":"e273d403-329f-11ee-a353-8c34c056f8ed","last_name":"Petrik"},{"first_name":"Aleksander","full_name":"Bena, Aleksander","id":"4197c39e-e8ec-11ed-86cb-afed934cd664","last_name":"Bena"},{"full_name":"Baunis, Haralds","first_name":"Haralds","last_name":"Baunis","id":"2eea55ec-e8ec-11ed-86cb-d9c76787acfe"},{"last_name":"Kelch","full_name":"Kelch, Riley M.","first_name":"Riley M."},{"first_name":"Tehshik P.","full_name":"Yoon, Tehshik P.","last_name":"Yoon"},{"full_name":"Pieber, Bartholomäus","orcid":"0000-0001-8689-388X","first_name":"Bartholomäus","last_name":"Pieber","id":"93e5e5b2-0da6-11ed-8a41-af589a024726"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["540"],"OA_place":"publisher","article_type":"original","file":[{"relation":"main_file","file_name":"2026_AdvSynthCatal_Petrik.pdf","date_created":"2026-05-07T07:29:24Z","file_id":"21833","date_updated":"2026-05-07T07:29:24Z","content_type":"application/pdf","success":1,"file_size":437184,"access_level":"open_access","checksum":"afe9752977898642c903abdc70b4a283","creator":"dernst"}],"PlanS_conform":"1","date_created":"2026-05-03T22:01:36Z","article_number":"e70417","quality_controlled":"1","oa":1,"has_accepted_license":"1","publisher":"Wiley","_id":"21776","oa_version":"Published Version","article_processing_charge":"Yes (via OA deal)","publication":"Advanced Synthesis & Catalysis","corr_author":"1","day":"05","language":[{"iso":"eng"}],"acknowledgement":"We gratefully acknowledge ISTA for generous financial support. B.P. acknowledges the Austrian Science Fund (PAT 1250924) and the ACS GCI Pharmaceutical Roundtable for funding; T.P.Y acknowledges the NSF(CHE-2349003) for financial support. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Lab Support Facility, Mass Spec Facility, NMR facility, and the Miba Machine Shop. We specifically thank Aikaterina Paraskevopoulou for HRMS measurements and Jan Pecak for support with ICP-OES experi-ments. NMR facilities at UW−Madison were supported by the NSF(CHE-1048642) and a generous gift from Paul J. and Margaret M. Bender. Open Access funding provided by Institute of Science and Technology Austria/KEMÖ. This study was supported by Austrian Science Fund (PAT 1250924), ACSGCI Pharmaceutical Roundtable, and National Science Foundation(CHE-2349003) and (CHE-1048642).","project":[{"grant_number":"PAT 1250924","_id":"8f1d607d-16d5-11f0-9cad-ab453295ba5e","name":"Photoactive ligands for transformative nickel catalysis"}],"department":[{"_id":"BaPi"},{"_id":"GradSch"}],"doi":"10.1002/adsc.70417","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"abstract":[{"lang":"eng","text":"Pyridyl motifs equipped with N-substituents can be powerful ligands for catalysis, yet their broader adoption is limited by the lack of a practical method to prepare these scaffolds. We report a modular, robust, and versatile Buchwald–Hartwig amination protocol that enables the rapid synthesis of bipyridine, phenanthroline, terpyridine, and pybox ligands bearing dialkylamine, diarylamine, and heteroaromatic N-substituents. These conditions streamline ligand library synthesis and will facilitate systematic studies in catalysis and related applications."}],"fulldoi":"https://doi.org/10.1002/adsc.70417","scopus_import":"1","publication_status":"published","intvolume":"       368","citation":{"ieee":"A. Petrik, A. Bena, H. Baunis, R. M. Kelch, T. P. Yoon, and B. Pieber, “Facile access to N-substituted pyridyl ligands,” <i>Advanced Synthesis &#38; Catalysis</i>, vol. 368, no. 9. Wiley, 2026.","chicago":"Petrik, Adam, Aleksander Bena, Haralds Baunis, Riley M. Kelch, Tehshik P. Yoon, and Bartholomäus Pieber. “Facile Access to N-Substituted Pyridyl Ligands.” <i>Advanced Synthesis &#38; Catalysis</i>. Wiley, 2026. <a href=\"https://doi.org/10.1002/adsc.70417\">https://doi.org/10.1002/adsc.70417</a>.","ista":"Petrik A, Bena A, Baunis H, Kelch RM, Yoon TP, Pieber B. 2026. Facile access to N-substituted pyridyl ligands. Advanced Synthesis &#38; Catalysis. 368(9), e70417.","apa":"Petrik, A., Bena, A., Baunis, H., Kelch, R. M., Yoon, T. P., &#38; Pieber, B. (2026). Facile access to N-substituted pyridyl ligands. <i>Advanced Synthesis &#38; Catalysis</i>. Wiley. <a href=\"https://doi.org/10.1002/adsc.70417\">https://doi.org/10.1002/adsc.70417</a>","ama":"Petrik A, Bena A, Baunis H, Kelch RM, Yoon TP, Pieber B. Facile access to N-substituted pyridyl ligands. <i>Advanced Synthesis &#38; Catalysis</i>. 2026;368(9). doi:<a href=\"https://doi.org/10.1002/adsc.70417\">10.1002/adsc.70417</a>","mla":"Petrik, Adam, et al. “Facile Access to N-Substituted Pyridyl Ligands.” <i>Advanced Synthesis &#38; Catalysis</i>, vol. 368, no. 9, e70417, Wiley, 2026, doi:<a href=\"https://doi.org/10.1002/adsc.70417\">10.1002/adsc.70417</a>.","short":"A. Petrik, A. Bena, H. Baunis, R.M. Kelch, T.P. Yoon, B. Pieber, Advanced Synthesis &#38; Catalysis 368 (2026)."},"date_published":"2026-05-05T00:00:00Z","title":"Facile access to N-substituted pyridyl ligands","type":"journal_article","month":"05","status":"public","OA_type":"hybrid","year":"2026","issue":"9","publication_identifier":{"eissn":["1615-4169"],"issn":["1615-4150"]},"volume":368,"date_updated":"2026-05-07T07:33:33Z","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"MassSpec"},{"_id":"NMR"},{"_id":"M-Shop"}],"file_date_updated":"2026-05-07T07:29:24Z"},{"status":"public","month":"05","type":"journal_article","OA_type":"gold","citation":{"ieee":"M. Li <i>et al.</i>, “Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants,” <i>Science Advances</i>, vol. 12, no. 19. AAAS, 2026.","chicago":"Li, Mingyue, Monika Chodasiewicz, Malavika Muraleedharan, Israel M. Lopez, Michal Gorka, Olga Kerber, Saqer S. Alotaibi, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” <i>Science Advances</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/sciadv.aea7828\">https://doi.org/10.1126/sciadv.aea7828</a>.","mla":"Li, Mingyue, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” <i>Science Advances</i>, vol. 12, no. 19, aea7828, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aea7828\">10.1126/sciadv.aea7828</a>.","short":"M. Li, M. Chodasiewicz, M. Muraleedharan, I.M. Lopez, M. Gorka, O. Kerber, S.S. Alotaibi, A.D.L. Nelson, R. Lenobel, J. Friedecká, A. Skirycz, J. Friml, Science Advances 12 (2026).","ista":"Li M, Chodasiewicz M, Muraleedharan M, Lopez IM, Gorka M, Kerber O, Alotaibi SS, Nelson ADL, Lenobel R, Friedecká J, Skirycz A, Friml J. 2026. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. 12(19), aea7828.","ama":"Li M, Chodasiewicz M, Muraleedharan M, et al. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. <i>Science Advances</i>. 2026;12(19). doi:<a href=\"https://doi.org/10.1126/sciadv.aea7828\">10.1126/sciadv.aea7828</a>","apa":"Li, M., Chodasiewicz, M., Muraleedharan, M., Lopez, I. M., Gorka, M., Kerber, O., … Friml, J. (2026). Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.aea7828\">https://doi.org/10.1126/sciadv.aea7828</a>"},"intvolume":"        12","title":"Biogenesis and downstream effects of 3',5' and 2',3' cAMP isomers in plants","date_published":"2026-05-08T00:00:00Z","date_updated":"2026-06-02T14:36:41Z","file_date_updated":"2026-06-02T14:33:55Z","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"LifeSc"}],"year":"2026","issue":"19","volume":12,"publication_identifier":{"eissn":["2375-2548"]},"department":[{"_id":"JiFr"}],"acknowledgement":" We thank J. Chai and D. Yu for providing the MBP-fused L7TIR plasmid and K. Jaworski (Nicolaus Copernicus University) for the GST-­HpAC1 plasmid. We also thank M. Randuch and L. Fiedler for providing vectors for recombinant AFB5 and ADCY. We are also grateful to E. Dutkiewicz, L. Trübestein, N. Krasnici and A. Michaelis for excellent technical\r\nassistance. We acknowledge the support of the LSF Mass Spectrometry Service and the Lab\r\nSupport Facility at the Institute of Science and Technology Austria for their contributions,\r\nincluding consultation on size exclusion chromatography, LC/MS experimental design,\r\nmetabolomics sample preparation, LC/MS method optimization, data acquisition, raw data\r\nanalysis, and absolute quantification. This project is supported by the European\r\nResearch Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogram (101142681 CYNIPS) and Austrian Science Fund (FWF; P 37051-B), both to J.Friml.\r\nWe acknowledge the generous support of the Taif University Researchers Supporting\r\nProject: TURSP-­HC2022/02 and Max-Planck-Society to A.S. ","project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants"},{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"external_id":{"pmid":["42102187"]},"publication_status":"published","doi":"10.1126/sciadv.aea7828","fulldoi":"https://doi.org/10.1126/sciadv.aea7828","scopus_import":"1","abstract":[{"lang":"eng","text":"Cyclic adenosine monophosphate (cAMP) is a fundamental second messenger involved in diverse signaling pathways across both animals and plants. While the role of 3′,5′-cAMP has been extensively characterized, the biological significance of its structural isomer, 2′,3′-cAMP, remains largely unexplored, particularly in plants. Here, we show that 2′,3′-cAMP and 3′,5′-cAMP represent parallel signaling systems in Arabidopsis thaliana, with different enzymatic origins and largely distinct downstream effects. In vitro enzymatic assays show that plant adenylate cyclases (ACs), including AFB5 and HpAC1, produce specifically 3′,5′-cAMP from ATP, whereas the TIR domain of protein L7 also catalyzes the formation of 2′,3′-cAMP from RNA. Comprehensive multiomics analyses reveal that two isomers elicit distinct yet partially overlapping metabolic, proteomic, and transcriptional response: 2′,3′-cAMP activates broad, stress-adaptive gene expression reprogramming, while 3′,5′-cAMP fine-tunes responses related to nutrient status and cellular homeostasis. Our findings establish the existence of dual cAMP signaling systems in plants, each with specialized functions and provide insights into the complex regulatory networks governing plant physiology."}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"corr_author":"1","article_processing_charge":"Yes","_id":"21914","publisher":"AAAS","oa_version":"Published Version","publication":"Science Advances","language":[{"iso":"eng"}],"day":"08","article_type":"original","OA_place":"publisher","ddc":["580"],"file":[{"file_id":"21941","success":1,"date_updated":"2026-06-02T14:33:55Z","content_type":"application/pdf","relation":"main_file","date_created":"2026-06-02T14:33:55Z","file_name":"2026_ScienceAdv_Li2.pdf","checksum":"75b8ef2db078652c750e34e9cd98a808","creator":"dernst","file_size":2014452,"access_level":"open_access"}],"author":[{"first_name":"Mingyue","full_name":"Li, Mingyue","id":"01f96916-0235-11eb-9379-a323192643b7","last_name":"Li"},{"full_name":"Chodasiewicz, Monika","first_name":"Monika","last_name":"Chodasiewicz"},{"full_name":"Muraleedharan, Malavika","first_name":"Malavika","last_name":"Muraleedharan"},{"last_name":"Lopez","full_name":"Lopez, Israel M.","first_name":"Israel M."},{"last_name":"Gorka","full_name":"Gorka, Michal","first_name":"Michal"},{"full_name":"Kerber, Olga","first_name":"Olga","last_name":"Kerber"},{"last_name":"Alotaibi","full_name":"Alotaibi, Saqer S.","first_name":"Saqer S."},{"first_name":"Andrew D.L.","full_name":"Nelson, Andrew D.L.","last_name":"Nelson"},{"full_name":"Lenobel, Rene","first_name":"Rene","last_name":"Lenobel"},{"last_name":"Friedecká","first_name":"Jaroslava","full_name":"Friedecká, Jaroslava"},{"last_name":"Skirycz","full_name":"Skirycz, Aleksandra","first_name":"Aleksandra"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"quality_controlled":"1","has_accepted_license":"1","oa":1,"DOAJ_listed":"1","date_created":"2026-05-24T22:01:31Z","PlanS_conform":"1","article_number":"aea7828"},{"OA_place":"repository","ddc":["570"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Ana","orcid":"0000-0002-5615-5277","full_name":"Villalba Requena, Ana","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","last_name":"Villalba Requena"},{"last_name":"Beattie","id":"2E26DF60-F248-11E8-B48F-1D18A9856A87","full_name":"Beattie, Robert J","first_name":"Robert J","orcid":"0000-0002-8483-8753"},{"id":"48EA0138-F248-11E8-B48F-1D18A9856A87","last_name":"Pauler","orcid":"0000-0002-7462-0048","first_name":"Florian","full_name":"Pauler, Florian"},{"first_name":"Carmen","full_name":"Streicher, Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher"},{"last_name":"Miranda","id":"862A3C56-A8BF-11E9-B4FA-D9E3E5697425","full_name":"Miranda, Osvaldo","first_name":"Osvaldo","orcid":"0000-0001-6618-6889"},{"last_name":"Krausgruber","first_name":"Thomas","full_name":"Krausgruber, Thomas"},{"first_name":"Martin","full_name":"Senekowitsch, Martin","last_name":"Senekowitsch"},{"last_name":"Farlik","first_name":"Matthias","full_name":"Farlik, Matthias"},{"full_name":"Bock, Christoph","first_name":"Christoph","last_name":"Bock"},{"first_name":"Thomas","full_name":"Rülicke, Thomas","last_name":"Rülicke"},{"full_name":"Hippenmeyer, Simon","orcid":"0000-0003-2279-1061","first_name":"Simon","last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87"}],"ec_funded":1,"has_accepted_license":"1","oa":1,"date_created":"2026-06-09T08:08:18Z","publication":"bioRxiv","article_processing_charge":"No","oa_version":"Preprint","_id":"21962","language":[{"iso":"eng"}],"day":"05","department":[{"_id":"SiHi"}],"acknowledgement":"We thank A. Heger (IST Austria Preclinical Facility), A. Sommer (VBCF GmbH, NGS Unit), and A.\r\nNicolas (IST Austria Lab Support Facility / Mass Spectrometry Facility) for technical support; K. Ferencak,\r\nI. Aykara, P. Hirschfeld, E. Fisher, S. Laukoter, L. Andersen for initial experiments and/or assistance; and\r\nall members of the Hippenmeyer lab for discussion. This research was supported by the Scientific Service\r\nUnits (SSU) of IST Austria through resources provided by the Imaging and Optics- (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). R.B. received support from FWF Meitner-Programm (M 2416). This\r\nwork was also supported by IST Austria institutional funds; the People Programme (Marie Curie Actions)\r\nof the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement\r\nNo 618444 to S.H., and the European Research Council (ERC) under the European Union’s Horizon 2020\r\nresearch and innovation programme (grant agreement No 725780 LinPro) to S.H.","project":[{"name":"Molecular Mechanisms Regulating Gliogenesis in the Neocortex","call_identifier":"FWF","_id":"264E56E2-B435-11E9-9278-68D0E5697425","grant_number":"M02416"},{"_id":"25D61E48-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"618444","name":"Molecular Mechanisms of Cerebral Cortex Development"},{"name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","_id":"260018B0-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"725780"}],"publication_status":"submitted","fulldoi":"https://doi.org/10.64898/2026.05.01.722172","abstract":[{"lang":"eng","text":"The generation of faithful cell-type diversity and correct projection neuron numbers is essential for cerebral cortex development. Corticogenesis is however susceptible to genetic interference of critical signaling pathways, including mutations in Mtor/Rptor that lead to microcephaly. How the loss of Rptor/mTORC1 function affects cortical developmental programs, at single cell level, is still unknown. Here, we utilized Mosaic Analysis with Double Markers (MADM) technology to probe Rptor gene function upon sparse single cell- or global tissue-wide ablation. We found that tissue-wide effects drive the etiology of cortical microcephaly upon loss of Rptor, rather than deficits in projection neuron genesis. Conversely, Rptor function is cell-autonomously required for postnatal projection neuron survival in a highly cell-type-specific manner. Collectively, our results suggest that the fine balance of precise cell-type-specific cell-autonomous Rptor/mTORC1 function in concert with non-cell-autonomous tissue-wide effects is essential for the development of a properly-sized cerebral cortex with accurate projection neuron diversity."}],"tmp":{"short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"doi":"10.64898/2026.05.01.722172","OA_type":"green","status":"public","type":"preprint","month":"05","title":"Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner","date_published":"2026-05-05T00:00:00Z","main_file_link":[{"url":"https://doi.org/10.64898/2026.05.01.722172","open_access":"1"}],"citation":{"ama":"Villalba Requena A, Beattie RJ, Pauler F, et al. Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>","ista":"Villalba Requena A, Beattie RJ, Pauler F, Streicher C, Miranda O, Krausgruber T, Senekowitsch M, Farlik M, Bock C, Rülicke T, Hippenmeyer S. Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. bioRxiv, <a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>.","apa":"Villalba Requena, A., Beattie, R. J., Pauler, F., Streicher, C., Miranda, O., Krausgruber, T., … Hippenmeyer, S. (n.d.). Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.05.01.722172\">https://doi.org/10.64898/2026.05.01.722172</a>","mla":"Villalba Requena, Ana, et al. “Mtor/Rptor Function Globally Prevents Cortical Microcephaly and Cell-Autonomously Promotes Postnatal Neuron Survival in Cell Type Specific Manner.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>.","short":"A. Villalba Requena, R.J. Beattie, F. Pauler, C. Streicher, O. Miranda, T. Krausgruber, M. Senekowitsch, M. Farlik, C. Bock, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.).","ieee":"A. Villalba Requena <i>et al.</i>, “Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner,” <i>bioRxiv</i>. .","chicago":"Villalba Requena, Ana, Robert J Beattie, Florian Pauler, Carmen Streicher, Osvaldo Miranda, Thomas Krausgruber, Martin Senekowitsch, et al. “Mtor/Rptor Function Globally Prevents Cortical Microcephaly and Cell-Autonomously Promotes Postnatal Neuron Survival in Cell Type Specific Manner.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.05.01.722172\">https://doi.org/10.64898/2026.05.01.722172</a>."},"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"MassSpec"},{"_id":"Bio"}],"date_updated":"2026-06-16T08:45:25Z","year":"2026"},{"year":"2026","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"date_updated":"2026-06-16T08:57:20Z","title":"Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production","date_published":"2026-05-05T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.64898/2026.05.01.722191"}],"citation":{"mla":"Miranda, Osvaldo, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>.","short":"O. Miranda, X. Contreras, F. Pauler, A. Davaatseren, N. Amberg, C. Streicher, A. Villalba Requena, A.-M. Heger, C. Marie, B.A. Hassan, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.).","ama":"Miranda O, Contreras X, Pauler F, et al. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>","apa":"Miranda, O., Contreras, X., Pauler, F., Davaatseren, A., Amberg, N., Streicher, C., … Hippenmeyer, S. (n.d.). Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.05.01.722191\">https://doi.org/10.64898/2026.05.01.722191</a>","ista":"Miranda O, Contreras X, Pauler F, Davaatseren A, Amberg N, Streicher C, Villalba Requena A, Heger A-M, Marie C, Hassan BA, Rülicke T, Hippenmeyer S. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv, <a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>.","ieee":"O. Miranda <i>et al.</i>, “Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production,” <i>bioRxiv</i>. .","chicago":"Miranda, Osvaldo, Ximena Contreras, Florian Pauler, Amarbayasgalan Davaatseren, Nicole Amberg, Carmen Streicher, Ana Villalba Requena, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.05.01.722191\">https://doi.org/10.64898/2026.05.01.722191</a>."},"OA_type":"green","status":"public","month":"05","type":"preprint","fulldoi":"https://doi.org/10.64898/2026.05.01.722191","tmp":{"short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"abstract":[{"text":"The cerebral cortex consists of immense numbers of neuronal and glial cell-types derived from radial glial progenitor (RGP) cells. How RGPs generate appropriate quantities of distinct cortical cell-types to safeguard a brain of correct size, is not well understood. However, genetic aberration in human, including mutations in PTEN, lead to cortical malformation such as macrocephaly, albeit with unknown etiology. Here we utilized Mosaic Analysis with Double Markers (MADM)-based clonal analysis and single cell phenotyping to decipher the role of Pten in neurogenic and gliogenic RGP lineage progression during cortical ontogeny. While neurogenic RGP lineage progression and projection neuron production was moderately altered in the absence of Pten, cortical astrocyte production was drastically increased. Through genetic epistasis experiments we show that the loss of Pten uncouples astrocyte generation from essential growth factor signaling hubs, funneling into MAPK. Collectively, our results suggest that Pten regulates RGP lineage progression with distinct sequential functions in cortical projection neurogenesis and astrocyte production to ensure the emergence of a correctly-sized cerebral cortex.","lang":"eng"}],"doi":"10.64898/2026.05.01.722191","publication_status":"submitted","project":[{"grant_number":"F7805","_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression"},{"_id":"260018B0-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"725780","name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development"}],"acknowledgement":"We thank Kay-Uwe Wagner (Wayne State University) for generously sharing Jak1/2–flox mouse lines; A.\r\nSommer (VBCF GmbH, NGS Unit) for technical support; N. Kim, V. Mick, S. Schnabl, S. Gobeil, and L.\r\nAndersen for technical assistance; all members of the Hippenmeyer lab for discussion and B. Novitch for\r\ncomments on earlier versions of the manuscript. This research was supported by the Scientific Service Units\r\n(SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). O.A.M received support from the Austrian Academy of Sciences\r\nÖAW (DOC 186584), and N.A. from FWF Elise Richter Program (Grant V1041T). This work was also\r\nsupported by IST Austria institutional funds; FWF SFB F78 (Neuro Stem Modulation) to S.H., and the\r\nEuropean Research Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogramme (grant agreement No 725780 LinPro) to S.H.","department":[{"_id":"SiHi"},{"_id":"PreCl"},{"_id":"GradSch"}],"day":"05","language":[{"iso":"eng"}],"publication":"bioRxiv","article_processing_charge":"No","oa_version":"Preprint","_id":"21963","corr_author":"1","date_created":"2026-06-09T08:08:53Z","has_accepted_license":"1","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"orcid":"0000-0001-6618-6889","first_name":"Osvaldo","full_name":"Miranda, Osvaldo","id":"862A3C56-A8BF-11E9-B4FA-D9E3E5697425","last_name":"Miranda"},{"last_name":"Contreras","id":"475990FE-F248-11E8-B48F-1D18A9856A87","full_name":"Contreras, Ximena","first_name":"Ximena"},{"first_name":"Florian","orcid":"0000-0002-7462-0048","full_name":"Pauler, Florian","id":"48EA0138-F248-11E8-B48F-1D18A9856A87","last_name":"Pauler"},{"last_name":"Davaatseren","id":"70ADC922-B424-11E9-99E3-BA18E6697425","full_name":"Davaatseren, Amarbayasgalan","first_name":"Amarbayasgalan"},{"last_name":"Amberg","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","full_name":"Amberg, Nicole","first_name":"Nicole","orcid":"0000-0002-3183-8207"},{"first_name":"Carmen","full_name":"Streicher, Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher"},{"last_name":"Villalba Requena","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","full_name":"Villalba Requena, Ana","orcid":"0000-0002-5615-5277","first_name":"Ana"},{"id":"4B76FFD2-F248-11E8-B48F-1D18A9856A87","last_name":"Heger","first_name":"Anna-Magdalena","full_name":"Heger, Anna-Magdalena"},{"last_name":"Marie","first_name":"Corentine","full_name":"Marie, Corentine"},{"last_name":"Hassan","full_name":"Hassan, Bassem A.","first_name":"Bassem A."},{"first_name":"Thomas","full_name":"Rülicke, Thomas","last_name":"Rülicke"},{"full_name":"Hippenmeyer, Simon","first_name":"Simon","orcid":"0000-0003-2279-1061","last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87"}],"ec_funded":1,"OA_place":"repository","ddc":["570"]},{"acknowledgement":"We thank all members of the Heisenberg group for discussion and feedback on the manuscript, and the Imaging and Optics Facility, the Life Science Support Facility and the Electron Microscopy Facility of the Institute of Science and Technology Austria (ISTA) for their continued support. We are grateful to M. Sonawane (Tata Institute of Fundamental Research, India) for providing the pCS2-HA-aPKC (PKCι)-V260F (DN) and pCS2-HA-aPKC (PKCι)-A122E (CA) plasmids, and to I. Mayer for the discussion. Molecular graphics and analyses were performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases. This research was funded in whole or in part by the Austrian Science Fund (FWF; grant no. PAT5044023) to C.-P.H., and by a JSPS Overseas Research Fellowship and an EMBO Postdoctoral Fellowship (ALTF 16-2022) to N.H.","project":[{"_id":"8f060199-16d5-11f0-9cad-f3253b266c46","grant_number":"PAT 5044023","name":"Keratins in epithelial tissue spreading"},{"_id":"34dd7f3b-11ca-11ed-8bc3-856f2c87f5da","grant_number":"LTF 16-2022","name":"Mechanosensitive signaling activation in the crosstalk between mechanical force and tissuefluidity"}],"department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"GradSch"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"abstract":[{"lang":"eng","text":"Tissue tension is a key determinant of tissue shape, and its regulation is essential for both morphogenesis and the maintenance of tissue integrity. During zebrafish embryogenesis, the enveloping layer (EVL) – an epithelial monolayer covering the blastoderm – undergoes extensive spreading that is driven by pulling forces exerted at its margin and more than doubles its surface area. Yet whether and how the EVL actively regulates its tissue tension during this process remains unclear. Here, we show that the EVL maintains constant tissue tension while spreading, and that it achieves this by reducing apical cell contractility in response to the same pulling forces that drive its spreading. We identify a mechanosensitive pathway underlying this response, mediated by the scaffold/adaptor protein Kibra regulating the activity of atypical protein kinase C (aPKC) at the apical domain of EVL cells. Under low mechanical stretch, Kibra forms condensates at the base of actin-based apical projections, where it activates Myosin II to increase apical contractility through aPKC downregulation. As mechanical stretch increases, apical projections disassemble, Kibra condensates dissolve, and aPKC activity rises. Elevated aPKC activity in turn reduces apical contractility by reducing Myosin II activity, thereby maintaining constant tissue tension despite increased mechanical stretch. Together, these findings reveal a mechanosensitive mechanism that enables robust adaptation of tissue tension to changing mechanical stretch, ensuring efficient tissue spreading and morphogenesis."}],"publication_status":"draft","citation":{"ama":"Hino N, Kapoor T, Gubbala UR, Hannezo EB, Heisenberg C-PJ. Apical domain mechanosensation regulates tissue tension homeostasis.","ista":"Hino N, Kapoor T, Gubbala UR, Hannezo EB, Heisenberg C-PJ. Apical domain mechanosensation regulates tissue tension homeostasis.","apa":"Hino, N., Kapoor, T., Gubbala, U. R., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (n.d.). Apical domain mechanosensation regulates tissue tension homeostasis. Institute of Science and Technology Austria.","mla":"Hino, Naoya, et al. <i>Apical Domain Mechanosensation Regulates Tissue Tension Homeostasis</i>. Institute of Science and Technology Austria.","short":"N. Hino, T. Kapoor, U.R. Gubbala, E.B. Hannezo, C.-P.J. Heisenberg, (n.d.).","ieee":"N. Hino, T. Kapoor, U. R. Gubbala, E. B. Hannezo, and C.-P. J. Heisenberg, “Apical domain mechanosensation regulates tissue tension homeostasis.” Institute of Science and Technology Austria.","chicago":"Hino, Naoya, Tushna Kapoor, Uday R Gubbala, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Apical Domain Mechanosensation Regulates Tissue Tension Homeostasis.” Institute of Science and Technology Austria, n.d."},"date_published":"2026-07-14T00:00:00Z","dataavailabilitystatement":"The MATLAB code for image analysis, and the full model code, including all parameter values\r\nand condition-specific settings, are available on GitHub at https://github.com/uday2607/EVL-tension-homeostasis.git.","title":"Apical domain mechanosensation regulates tissue tension homeostasis","type":"preprint","month":"07","status":"public","OA_type":"green","year":"2026","keyword":["Epithelial spreading","tissue tension","mechanosensation","aPKC","Kibra","zebrafish"],"supplementarymaterial":"yes","date_updated":"2026-07-14T07:07:41Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"file_date_updated":"2026-07-13T09:16:28Z","author":[{"first_name":"Naoya","full_name":"Hino, Naoya","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","last_name":"Hino"},{"last_name":"Kapoor","id":"e3b3eda7-fd4d-11eb-8fd8-c40af7a478b1","full_name":"Kapoor, Tushna","first_name":"Tushna"},{"id":"bb4a0dc4-32c9-11ee-b5ce-a97ceedd5924","last_name":"Gubbala","first_name":"Uday R","full_name":"Gubbala, Uday R"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561","first_name":"Edouard B","full_name":"Hannezo, Edouard B"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"OA_place":"publisher","file":[{"relation":"main_file","date_created":"2026-07-13T09:16:20Z","file_name":"Main_text_and_figures.pdf","file_id":"22283","success":1,"content_type":"application/pdf","date_updated":"2026-07-13T09:16:20Z","file_size":12477675,"access_level":"open_access","checksum":"66444afd243dce7d383d52d44e8d34a4","creator":"nhino"},{"file_id":"22284","content_type":"application/pdf","date_updated":"2026-07-13T09:16:25Z","success":1,"relation":"main_file","file_name":"Supplementary_figures.pdf","date_created":"2026-07-13T09:16:25Z","checksum":"90bceb34de64ec792c5de117f0890d05","creator":"nhino","file_size":4545901,"access_level":"open_access"},{"checksum":"9d9ab89c372142f2ffb6c8c625334d7f","creator":"nhino","file_size":10349451,"access_level":"open_access","file_id":"22285","content_type":"video/mp4","date_updated":"2026-07-13T09:16:28Z","success":1,"relation":"main_file","file_name":"Supplementary_Video1.mp4","date_created":"2026-07-13T09:16:28Z"}],"date_created":"2026-07-13T09:03:26Z","oa":1,"has_accepted_license":"1","oa_version":"Preprint","_id":"22276","publisher":"Institute of Science and Technology Austria","article_processing_charge":"No","researchdata_availability":"yes","corr_author":"1","day":"14","related_material":{"record":[{"status":"public","id":"21864","relation":"earlier_version"}]},"das_tickbox":"1","language":[{"iso":"eng"}]},{"corr_author":"1","researchdata_availability":"yes","publication":"Nature Communications","article_processing_charge":"Yes","publisher":"Springer Nature","_id":"22333","oa_version":"Published Version","language":[{"iso":"eng"}],"das_tickbox":"1","day":"13","OA_place":"publisher","article_type":"original","ddc":["570"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"id":"36062FEC-F248-11E8-B48F-1D18A9856A87","last_name":"Hlavata","first_name":"Annamaria","full_name":"Hlavata, Annamaria"},{"first_name":"Benjamin","full_name":"Neuditschko, Benjamin","last_name":"Neuditschko"},{"first_name":"Ulla","full_name":"Schellhaas, Ulla","last_name":"Schellhaas"},{"last_name":"Plaschka","full_name":"Plaschka, Clemens","first_name":"Clemens"},{"full_name":"Herzog, Franz","first_name":"Franz","last_name":"Herzog"},{"id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","last_name":"Bernecky","first_name":"Carrie A","orcid":"0000-0003-0893-7036","full_name":"Bernecky, Carrie A"}],"has_accepted_license":"1","oa":1,"quality_controlled":"1","DOAJ_listed":"1","date_created":"2026-07-14T07:27:59Z","PlanS_conform":"1","OA_type":"gold","status":"public","type":"journal_article","month":"07","dataavailabilitystatement":"The\r\nc ryo EM maps generated in this study were deposited to the EM Data Bank under the\r\naccession codes: EMD 55583 [https://www.ebi.ac.uk/pdbe/entry/emdb/EMD 55583\r\n(Pol II Gdown1 RPAP2 composite map), EMD 55578\r\n[https://www.ebi.ac.uk/pdbe/entry/emdb/EMD 55578 Pol II Gdown1 RPAP2 Pol II core\r\nmap EMD 55579 [https://www.ebi.ac.uk/pdbe/entry/emdb/EMD 55579 Pol II\r\nGdown1 RPAP2 Pol II stalk map EMD 55580\r\n[https://www.ebi.ac.uk/pdbe/entry/emdb/EMD 55580 Pol II Gdown1 RPAP2 RPAP2\r\nmap EMD 55581 [https://www.ebi.ac.uk/pdbe/entry/emdb/EMD 55 581 Pol II\r\nGdown1 RPAP2 Gdown1 N terminus map EMD 55582\r\n[https://www.ebi.ac.uk/pdbe/entry/emdb/EMD 55582 Pol II Gdown1 RPAP2 Gdown1\r\nC terminus map and EMD 55585 [https://www.ebi.ac.uk/pdbe/entry/emdb/EMD\r\n55585 RPAP2 GPN1 GPN3 map Model coordi nates were deposited to the PDBe under\r\nthe accession codes: 9T5H [http://doi.org/10.2210/pdb 9T5H / (Pol II Gdown1\r\nRPAP2 complex structure) and 9T5J [http://doi.org/10.2210/pdb 9T5H / (GPN1\r\nGPN3 RPAP2 structure). Immunoprecipitation mass spectrometry and crosslinking mass\r\nspectrometry proteomics data have been deposited to the ProteomeXchange Consortium\r\nvia the PRIDE partner repository with the dataset identifiers PXD071638\r\n[http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD 071638 and\r\nP XD070852\r\n[http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD 070852\r\nAlphaFold3 structure predictions have been deposited to the Zenodo repository\r\nhttps://doi.org/10.5281/zenodo.20687910 P reviously published model coordinates\r\nwere utilized and are available at the PDB under the accession codes 8QEP\r\n[http://doi.org/10.2210/pdb 8QEP / 9BZ 0 [http://doi.org/10.2210/pdb 9BZ 0 /\r\nand 7B7U [http://doi.org/10.2210/pdb 7B7U / Source Data are provided with this\r\npaper.","title":"Structure of cytoplasmic RNA polymerase II","date_published":"2026-07-13T00:00:00Z","main_file_link":[{"url":"https://doi.org/10.1038/s41467-026-75416-8","open_access":"1"}],"citation":{"chicago":"Hlavata, Annamaria, Benjamin Neuditschko, Ulla Schellhaas, Clemens Plaschka, Franz Herzog, and Carrie Bernecky. “Structure of Cytoplasmic RNA Polymerase II.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-75416-8\">https://doi.org/10.1038/s41467-026-75416-8</a>.","ieee":"A. Hlavata, B. Neuditschko, U. Schellhaas, C. Plaschka, F. Herzog, and C. Bernecky, “Structure of cytoplasmic RNA polymerase II,” <i>Nature Communications</i>. Springer Nature, 2026.","ista":"Hlavata A, Neuditschko B, Schellhaas U, Plaschka C, Herzog F, Bernecky C. 2026. Structure of cytoplasmic RNA polymerase II. Nature Communications.","apa":"Hlavata, A., Neuditschko, B., Schellhaas, U., Plaschka, C., Herzog, F., &#38; Bernecky, C. (2026). Structure of cytoplasmic RNA polymerase II. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-75416-8\">https://doi.org/10.1038/s41467-026-75416-8</a>","ama":"Hlavata A, Neuditschko B, Schellhaas U, Plaschka C, Herzog F, Bernecky C. Structure of cytoplasmic RNA polymerase II. <i>Nature Communications</i>. 2026. doi:<a href=\"https://doi.org/10.1038/s41467-026-75416-8\">10.1038/s41467-026-75416-8</a>","short":"A. Hlavata, B. Neuditschko, U. Schellhaas, C. Plaschka, F. Herzog, C. Bernecky, Nature Communications (2026).","mla":"Hlavata, Annamaria, et al. “Structure of Cytoplasmic RNA Polymerase II.” <i>Nature Communications</i>, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-75416-8\">10.1038/s41467-026-75416-8</a>."},"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"EM-Fac"},{"_id":"ScienComp"},{"_id":"PreCl"}],"date_updated":"2026-07-16T11:29:31Z","publication_identifier":{"eissn":["2041-1723"]},"supplementarymaterial":"yes","year":"2026","biorxivid":1,"department":[{"_id":"CaBe"}],"acknowledgement":"We thank A. Salmazo for assistance with Pol II purification. We thank staff at the Vienna BioCenter Core Facilities (VBCF) Proteomics facility for immunoprecipitation-mass spectrometry analysis, and J.A. Stopp for assistance with IP-MS data visualization. This research was further supported by the Scientific Service Units (SSUs) of ISTA through resources provided by the Lab Support Facility (LSF), Electron Microscopy Facility (EMF), Scientific Computing (SciComp), and the Preclinical Facility (PCF). F.H. was funded by the Endowed Professorship of the Lower Austria Research Funding Agency (GFF NÖ) and by the Austrian Research Promotion Agency (FFG) through the COIN Establishment Grant n.o. 45624401.","external_id":{"biorxivid":["10.64898/2025.12.10.692585"]},"publication_status":"epub_ahead","scopus_import":"1","fulldoi":"https://doi.org/10.1038/s41467-026-75416-8","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"abstract":[{"text":"RNA polymerase II (Pol II) must be assembled in the cytoplasm before it enters the nucleus, where it transcribes protein-coding genes. Although transcription by Pol II is intensively studied, how this central multi-subunit enzyme is made and the role of dedicated assembly factors remains unclear. Here, we report the integrative structural analysis of a native human Pol II from the cytoplasm captured near the end of biogenesis. The complex contains Gdown1 and three biogenesis factors – RPAP2 and the critical small GTPases GPN1 and GPN3. Cryo-EM analysis of the complex reveals how Gdown1 and RPAP2 associate with Pol II and prevent the premature association of transcription factors. Further biochemical and cryo-EM analysis reveals how RPAP2 tethers GPN1–GPN3 to the complex and how the assembly of the RPAP2–GPN1–GPN3 complex is controlled by GTP hydrolysis. The combined results uncover a network of interactions that chaperone cytoplasmic Pol II to prevent aberrant interactions, reveal a molecular switch regulating biogenesis factor association, and suggest a general mechanism for the action of GPN-loop GTPase family of enzymes.","lang":"eng"}],"doi":"10.1038/s41467-026-75416-8"},{"day":"16","language":[{"iso":"eng"}],"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"22334"}]},"publication":"Magnetic Resonance","article_processing_charge":"Yes","_id":"21777","oa_version":"Published Version","publisher":"Copernicus Publications","corr_author":"1","DOAJ_listed":"1","date_created":"2026-05-03T22:01:36Z","PlanS_conform":"1","has_accepted_license":"1","oa":1,"quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"author":[{"orcid":"0000-0002-6401-5151","first_name":"Lea Marie","full_name":"Becker, Lea Marie","id":"36336939-eb97-11eb-a6c2-c83f1214ca79","last_name":"Becker"},{"id":"334a5e40-8747-11f0-b671-ba1f5154b4b4","last_name":"Toscano","first_name":"Giorgia","full_name":"Toscano, Giorgia"},{"full_name":"Kapitonova, Anna","first_name":"Anna","last_name":"Kapitonova","id":"9fb2a840-89e1-11ee-a8b7-cc5c7ba62471"},{"full_name":"Singh, Rajkumar","first_name":"Rajkumar","last_name":"Singh","id":"a3089acd-6806-11ee-bacc-f0c7d500ad20"},{"first_name":"Undina","full_name":"Guillerm, Undina","id":"bb74f472-ae54-11eb-9835-bc9c22fb1183","last_name":"Guillerm"},{"last_name":"Lichtenecker","full_name":"Lichtenecker, Roman J.","first_name":"Roman J."},{"orcid":"0000-0002-9350-7606","first_name":"Paul","full_name":"Schanda, Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","last_name":"Schanda"}],"OA_place":"publisher","article_type":"original","ddc":["540"],"volume":7,"publication_identifier":{"eissn":["2699-0016"]},"issue":"1","year":"2026","page":"29-37","acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"date_updated":"2026-07-20T09:49:12Z","title":"Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants","date_published":"2026-04-16T00:00:00Z","main_file_link":[{"url":"https://doi.org/10.5194/mr-7-29-2026","open_access":"1"}],"citation":{"ista":"Becker LM, Toscano G, Kapitonova A, Singh R, Guillerm U, Lichtenecker RJ, Schanda P. 2026. Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants. Magnetic Resonance. 7(1), 29–37.","apa":"Becker, L. M., Toscano, G., Kapitonova, A., Singh, R., Guillerm, U., Lichtenecker, R. J., &#38; Schanda, P. (2026). Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants. <i>Magnetic Resonance</i>. Copernicus Publications. <a href=\"https://doi.org/10.5194/mr-7-29-2026\">https://doi.org/10.5194/mr-7-29-2026</a>","ama":"Becker LM, Toscano G, Kapitonova A, et al. Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants. <i>Magnetic Resonance</i>. 2026;7(1):29-37. doi:<a href=\"https://doi.org/10.5194/mr-7-29-2026\">10.5194/mr-7-29-2026</a>","short":"L.M. Becker, G. Toscano, A. Kapitonova, R. Singh, U. Guillerm, R.J. Lichtenecker, P. Schanda, Magnetic Resonance 7 (2026) 29–37.","mla":"Becker, Lea Marie, et al. “Accelerated 19F Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.” <i>Magnetic Resonance</i>, vol. 7, no. 1, Copernicus Publications, 2026, pp. 29–37, doi:<a href=\"https://doi.org/10.5194/mr-7-29-2026\">10.5194/mr-7-29-2026</a>.","chicago":"Becker, Lea Marie, Giorgia Toscano, Anna Kapitonova, Rajkumar Singh, Undina Guillerm, Roman J. Lichtenecker, and Paul Schanda. “Accelerated 19F Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.” <i>Magnetic Resonance</i>. Copernicus Publications, 2026. <a href=\"https://doi.org/10.5194/mr-7-29-2026\">https://doi.org/10.5194/mr-7-29-2026</a>.","ieee":"L. M. Becker <i>et al.</i>, “Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants,” <i>Magnetic Resonance</i>, vol. 7, no. 1. Copernicus Publications, pp. 29–37, 2026."},"intvolume":"         7","OA_type":"gold","status":"public","type":"journal_article","month":"04","fulldoi":"https://doi.org/10.5194/mr-7-29-2026","scopus_import":"1","abstract":[{"lang":"eng","text":"The advantageous characteristics attributed to the 19F nucleus have made it a popular target for nuclear magnetic resonance (NMR) once again in recent years. Aside from solution NMR, an increasing number of studies have been conducted applying solid-state magic-angle spinning (MAS) NMR to fluorine-labelled samples. Here, the high chemical shift anisotropy and strong dipolar couplings can be utilised to get structural insights into proteins and measure long distances. Despite increasing popularity and promising benefits, the sensitivity of biomolecular 19F MAS NMR often suffers from slow longitudinal T1 relaxation and therefore long recycle delays. In this work, we expand paramagnetic doping, an approach commonly used to reduce proton T1 relaxation times, to 19F-labelled biological samples. We study the effect of Gd(DTPA) and Gd(DTPA-BMA) on 19F T1 and T2, and 13C T1 and T2 relaxation in a [5-19F13C]-tryptophan-labelled protein via 19F-detected MAS NMR experiments. The observed paramagnetic relaxation enhancement substantially reduces measurement times of 19F MAS NMR experiments without compromising resolution. Additionally, we report the chemical shift assignments of all four fluorotryptophan signals in the 12×39 kDa-large protein TET2 using a mutagenesis approach."}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"doi":"10.5194/mr-7-29-2026","external_id":{"pmid":["42057802"]},"publication_status":"published","acknowledgement":"We thank Ben P. Tatman for insightful discussions. This research was supported by the Scientific Service Units (SSUs) of ISTA through resources provided by the Nuclear Magnetic Resonance Facility and the Lab Support Facility. We thank Prof. Tobias Madl (Medical University Graz) for a sample of Omniscan. Lea M. Becker is the recipient of a DOC fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology Austria (grant no. PR10660EAW01).","project":[{"_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854","name":"IST Austria Open Access Fund"},{"_id":"7be609c4-9f16-11ee-852c-85015ce2b9b0","grant_number":"26777","name":"Exploring protein dynamics by solid-state MAS NMR through specific labeling approaches"}],"department":[{"_id":"PaSc"},{"_id":"GradSch"}]},{"date_updated":"2026-07-20T14:28:59Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"year":"2026","publication_identifier":{"eissn":["1469-3178"]},"supplementarymaterial":"yes","type":"journal_article","month":"07","status":"public","OA_type":"gold","citation":{"mla":"Dehio, Philippe G., et al. “A Conserved VPS34-PIKfyve-TRPML1-Myosin II Axis Regulates the Speed of Amoeboid Cell Migration.” <i>EMBO Reports</i>, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s44319-026-00861-x\">10.1038/s44319-026-00861-x</a>.","short":"P.G. Dehio, C. Michard, J.C. Yam-Puc, A.A. Martí I Líndez, A. Jandke, G. Unterstab, L. Fabre, L. Sauteur, M. Artinger, D.F. Legler, M.K. Sixt, T. Schaefer, M.P. Wymann, K. Okkenhaug, T. Soldati, M. Mehling, C. Hess, EMBO Reports (2026).","ista":"Dehio PG, Michard C, Yam-Puc JC, Martí I Líndez AA, Jandke A, Unterstab G, Fabre L, Sauteur L, Artinger M, Legler DF, Sixt MK, Schaefer T, Wymann MP, Okkenhaug K, Soldati T, Mehling M, Hess C. 2026. A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration. EMBO Reports.","ama":"Dehio PG, Michard C, Yam-Puc JC, et al. A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration. <i>EMBO Reports</i>. 2026. doi:<a href=\"https://doi.org/10.1038/s44319-026-00861-x\">10.1038/s44319-026-00861-x</a>","apa":"Dehio, P. G., Michard, C., Yam-Puc, J. C., Martí I Líndez, A. A., Jandke, A., Unterstab, G., … Hess, C. (2026). A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration. <i>EMBO Reports</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s44319-026-00861-x\">https://doi.org/10.1038/s44319-026-00861-x</a>","ieee":"P. G. Dehio <i>et al.</i>, “A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration,” <i>EMBO Reports</i>. Springer Nature, 2026.","chicago":"Dehio, Philippe G, Céline Michard, Juan Carlos Yam-Puc, Adrià Arnau Martí I Líndez, Anett Jandke, Gunhild Unterstab, Lucien Fabre, et al. “A Conserved VPS34-PIKfyve-TRPML1-Myosin II Axis Regulates the Speed of Amoeboid Cell Migration.” <i>EMBO Reports</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s44319-026-00861-x\">https://doi.org/10.1038/s44319-026-00861-x</a>."},"date_published":"2026-07-07T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s44319-026-00861-x"}],"title":"A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration","dataavailabilitystatement":"The analysis workflow to quantify vesicle localization can be accessed on GitHub (https://github.com/loicsauteur/vesicle-analysis, version 0.1.1).\r\n\r\nThe source data of this paper are collected in the following database record: biostudies:S-SCDT-10_1038-S44319-026-00861-x.","publication_status":"epub_ahead","external_id":{"pmid":["42414599"]},"doi":"10.1038/s44319-026-00861-x","abstract":[{"text":"Amoeboid cell migration is key to efficient T cell immunity. Spatial polarization of organelles within cells, including endo-lysosomes, is a prerequisite of migration. However, how ultrastructural polarization is linked to the signaling requirements governing T cell migration remains unknown. Here we show that signaling molecules generated by endo-lysosome-localized kinases regulate velocity of amoeboid migration. Specifically, imaging of T cells identifies accumulation of endo-lysosomes decorated with the lipid kinases VPS34–PIKfyve at the uropod of polarized cells. Activity of VPS34 and PIKfyve regulates speed, but not directedness, of migrating T cells. Mechanistically, PI(3,5)P2 generated by the sequential action of VPS34 and PIKfyve, mediates Ca2+ efflux from lysosomes via the mucolipin TRP cation channel 1 (TRPML1), thus controlling activity of myosin IIA and hence the generation of propulsive force through retrograde actin flow. The VPS34–PIKfyve kinases also regulate velocity of myeloid cells, as well as of the amoeba Dictyostelium discoideum – establishing the axis as an evolutionarily conserved speed control system of amoeboid cell migration.","lang":"eng"}],"scopus_import":"1","fulldoi":"https://doi.org/10.1038/s44319-026-00861-x","department":[{"_id":"MiSi"}],"acknowledgement":"We thank the microscopy core facility of the Department of Biomedicine at the University and University Hospital of Basel for their technical support. This research was technically supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). CH was supported by the Swiss National Science Foundation (SNSF) (310030B_201277; 310030_192677; FZEB-0-180487), the ZBF Program Award 2025 (Hans Zäslin Bustany Foundation), and the Novartis Foundation for Medical-Biological Research (NFMBR) (#23A070). PD was supported by the Swiss Academy for Medical Sciences (SAMW) and SNSF (183980, 225441), the NFMBR (#23A070), AlumniMedizin Basel, and the Freiwillige Akademische Gesellschaft Basel. DFL was supported by the SNSF (220205). Open access funding provided by University of Basel.","das_tickbox":"1","language":[{"iso":"eng"}],"day":"07","researchdata_availability":"yes","publisher":"Springer Nature","_id":"22371","oa_version":"Published Version","article_processing_charge":"Yes (via OA deal)","publication":"EMBO Reports","quality_controlled":"1","oa":1,"DOAJ_listed":"1","date_created":"2026-07-19T22:01:48Z","OA_place":"publisher","article_type":"original","author":[{"full_name":"Dehio, Philippe G","first_name":"Philippe G","last_name":"Dehio","id":"b769738e-a003-11ee-b1b8-9030316e0d59"},{"last_name":"Michard","first_name":"Céline","full_name":"Michard, Céline"},{"last_name":"Yam-Puc","first_name":"Juan Carlos","full_name":"Yam-Puc, Juan Carlos"},{"last_name":"Martí I Líndez","full_name":"Martí I Líndez, Adrià Arnau","first_name":"Adrià Arnau"},{"last_name":"Jandke","first_name":"Anett","full_name":"Jandke, Anett"},{"last_name":"Unterstab","full_name":"Unterstab, Gunhild","first_name":"Gunhild"},{"first_name":"Lucien","full_name":"Fabre, Lucien","last_name":"Fabre"},{"last_name":"Sauteur","first_name":"Loïc","full_name":"Sauteur, Loïc"},{"full_name":"Artinger, Marc","first_name":"Marc","last_name":"Artinger"},{"full_name":"Legler, Daniel F.","first_name":"Daniel F.","last_name":"Legler"},{"last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179"},{"first_name":"Thorsten","full_name":"Schaefer, Thorsten","last_name":"Schaefer"},{"full_name":"Wymann, Matthias P.","first_name":"Matthias P.","last_name":"Wymann"},{"last_name":"Okkenhaug","full_name":"Okkenhaug, Klaus","first_name":"Klaus"},{"full_name":"Soldati, Thierry","first_name":"Thierry","last_name":"Soldati"},{"full_name":"Mehling, Matthias","first_name":"Matthias","orcid":"0000-0001-8599-1226","last_name":"Mehling","id":"3C23B994-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hess","full_name":"Hess, Christoph","first_name":"Christoph"}],"pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"day":"06","das_tickbox":"1","language":[{"iso":"eng"}],"article_processing_charge":"No","publisher":"Royal Society of Chemistry","_id":"21730","oa_version":"None","publication":"Journal of Materials Chemistry B","researchdata_availability":"yes","corr_author":"1","date_created":"2026-04-13T07:45:26Z","quality_controlled":"1","author":[{"first_name":"Moumita","full_name":"Mondal, Moumita","last_name":"Mondal"},{"last_name":"Ghorai","first_name":"Pravat","full_name":"Ghorai, Pravat"},{"full_name":"Samadder, Asmita","first_name":"Asmita","last_name":"Samadder"},{"full_name":"Freunberger, Stefan Alexander","first_name":"Stefan Alexander","orcid":"0000-0003-2902-5319","last_name":"Freunberger","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425"},{"last_name":"Banerjee","full_name":"Banerjee, Priyabrata","first_name":"Priyabrata"}],"pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","issue":"17","year":"2026","page":"5314-5322","volume":14,"publication_identifier":{"issn":["2050-750X"],"eissn":["2050-7518"]},"supplementarymaterial":"yes","date_updated":"2026-07-27T12:36:05Z","acknowledged_ssus":[{"_id":"LifeSc"}],"citation":{"ista":"Mondal M, Ghorai P, Samadder A, Freunberger SA, Banerjee P. 2026. H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis. Journal of Materials Chemistry B. 14(17), 5314–5322.","apa":"Mondal, M., Ghorai, P., Samadder, A., Freunberger, S. A., &#38; Banerjee, P. (2026). H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis. <i>Journal of Materials Chemistry B</i>. Royal Society of Chemistry. <a href=\"https://doi.org/10.1039/d5tb02687c\">https://doi.org/10.1039/d5tb02687c</a>","ama":"Mondal M, Ghorai P, Samadder A, Freunberger SA, Banerjee P. H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis. <i>Journal of Materials Chemistry B</i>. 2026;14(17):5314-5322. doi:<a href=\"https://doi.org/10.1039/d5tb02687c\">10.1039/d5tb02687c</a>","mla":"Mondal, Moumita, et al. “H2O2 Responsive Rhodamine-Based Probe for Monitoring Early-Stage Diabetes Diagnosis.” <i>Journal of Materials Chemistry B</i>, vol. 14, no. 17, Royal Society of Chemistry, 2026, pp. 5314–22, doi:<a href=\"https://doi.org/10.1039/d5tb02687c\">10.1039/d5tb02687c</a>.","short":"M. Mondal, P. Ghorai, A. Samadder, S.A. Freunberger, P. Banerjee, Journal of Materials Chemistry B 14 (2026) 5314–5322.","ieee":"M. Mondal, P. Ghorai, A. Samadder, S. A. Freunberger, and P. Banerjee, “H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis,” <i>Journal of Materials Chemistry B</i>, vol. 14, no. 17. Royal Society of Chemistry, pp. 5314–5322, 2026.","chicago":"Mondal, Moumita, Pravat Ghorai, Asmita Samadder, Stefan Alexander Freunberger, and Priyabrata Banerjee. “H2O2 Responsive Rhodamine-Based Probe for Monitoring Early-Stage Diabetes Diagnosis.” <i>Journal of Materials Chemistry B</i>. Royal Society of Chemistry, 2026. <a href=\"https://doi.org/10.1039/d5tb02687c\">https://doi.org/10.1039/d5tb02687c</a>."},"intvolume":"        14","dataavailabilitystatement":"The data supporting this article have been included as part of the supplementary information (SI). The supplementary information includes all spectral profiles, plots and tabulated data. See DOI: https://doi.org/10.1039/d5tb02687c.","title":"H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis","date_published":"2026-05-06T00:00:00Z","status":"public","type":"journal_article","month":"05","OA_type":"closed access","doi":"10.1039/d5tb02687c","fulldoi":"https://doi.org/10.1039/d5tb02687c","scopus_import":"1","abstract":[{"lang":"eng","text":"Hydrogen peroxide (H2O2) is a crucial member of the reactive oxygen species (ROS) family, playing roles in cellular signalling and immune responses in human health. Moreover, it is a potential biomarker of diabetes when present in aberrant concentrations. Therefore, monitoring trace levels of H2O2 has become a research hotspot for analytical and sensor chemists. In this context, we report a rhodamine-based fluorescent probe (RN), which shows excellent fluorescent enhancement at 555 nm upon the addition of H2O2 along with a low limit of detection (LOD) of 0.67 ppm and fast response (∼2 min). The probe is highly selective for H2O2, showing no fluorescence enhancement with other ROS. RN is synthesised in a one-pot chemical reaction using rhodamine 6G (R6G) and 4,7,10-trioxa-1,13-tridecanediamine (TTDA). H2O2 detection in pre-treated milk samples proves its real-world viability. We found that RN shows low cytotoxicity, which allowed us to successfully explore its potential to monitor H2O2 generation in a diabetic L929 skin cell line and diabetic mice liver tissue. This result demonstrates promising features for assessing early diabetic progression through fluorescence imaging."}],"external_id":{"pmid":["41958432"]},"publication_status":"published","acknowledgement":"MM acknowledges the Government of India for DST-INSPIRE\r\nfellowship [IF200389] and Federal Ministry of Education, Science and Research (BMBWF) and the OeAD – Austria’s Agency for Education and Internationalisation for an Ernst Mach Grant, weltweit (grant number MPC-2024-01518) for research internship at ISTA. The Scientific Service Units of ISTA supported this research through resources provided by the Lab Support Facility. PG acknowledges the ANRF, India, for his NPDF fellowship (File no. PDF/2022/001960). PB acknowledges ANRF, India, for the SERB-CRG sponsored project GAP-240712 (vide reference no. CRG/2022/001679).","department":[{"_id":"StFr"}]},{"day":"26","language":[{"iso":"eng"}],"related_material":{"record":[{"id":"21363","status":"public","relation":"research_data"}]},"das_tickbox":"1","alternative_title":["ISTA Thesis"],"oa_version":"Published Version","_id":"21360","publisher":"Institute of Science and Technology Austria","doi_confirm":"1","article_processing_charge":"No","corr_author":"1","degree_awarded":"PhD","date_created":"2026-02-27T09:08:14Z","has_accepted_license":"1","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","author":[{"last_name":"Riegler","id":"FF6018E0-D806-11E9-8E43-0B14E6697425","full_name":"Riegler, Stefan","orcid":"0000-0003-3413-1343","first_name":"Stefan"}],"file":[{"relation":"source_file","file_name":"2026_Riegler_Stefan_Thesis.zip","date_created":"2026-03-02T10:59:50Z","file_id":"21386","date_updated":"2026-03-02T10:59:50Z","content_type":"application/x-zip-compressed","file_size":31430022,"access_level":"closed","checksum":"2f1f44e8536c2538f94a440217452c9f","creator":"sriegler"},{"access_level":"closed","embargo_to":"open_access","file_size":11635090,"creator":"sriegler","checksum":"2e8dc39640bc26ae5684c944c619719b","file_name":"2026_Riegler_Stefan_Thesis.pdf","date_created":"2026-03-02T10:59:49Z","relation":"main_file","embargo":"2027-02-27","content_type":"application/pdf","date_updated":"2026-03-02T10:59:49Z","file_id":"21387"}],"ddc":["570","575","583"],"OA_place":"repository","supervisor":[{"last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739"}],"publication_identifier":{"issn":["2663-337X"]},"page":"185","year":"2026","file_date_updated":"2026-03-02T10:59:50Z","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"date_updated":"2026-07-27T14:30:08Z","date_published":"2026-02-26T00:00:00Z","title":"Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species","citation":{"ieee":"S. Riegler, “Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species,” Institute of Science and Technology Austria, 2026.","chicago":"Riegler, Stefan. “Root System Plasticity under Nutrient Limitation: Investigating Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea  Species.” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-21360\">https://doi.org/10.15479/AT-ISTA-21360</a>.","ista":"Riegler S. 2026. Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species. Institute of Science and Technology Austria.","apa":"Riegler, S. (2026). <i>Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-21360\">https://doi.org/10.15479/AT-ISTA-21360</a>","ama":"Riegler S. Root system plasticity under nutrient limitation: Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea  species. 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21360\">10.15479/AT-ISTA-21360</a>","mla":"Riegler, Stefan. <i>Root System Plasticity under Nutrient Limitation: Investigating Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea  Species</i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21360\">10.15479/AT-ISTA-21360</a>.","short":"S. Riegler, Root System Plasticity under Nutrient Limitation: Investigating Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea  Species, Institute of Science and Technology Austria, 2026."},"month":"02","type":"dissertation","status":"public","tmp":{"short":"CC BY-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","image":"/images/cc_by_sa.png"},"fulldoi":"https://doi.org/10.15479/AT-ISTA-21360","doi":"10.15479/AT-ISTA-21360","publication_status":"published","project":[{"name":"Breeding for coffee and cocoa root resilience in low input farming systems based on improved rootstocks","grant_number":"101060393","_id":"34afa094-11ca-11ed-8bc3-a375845a59fb"}],"acknowledgement":"I would like to acknowledge the Austrian Academy of Sciences (ÖAW) and European\r\nResearch Executive Agency (REA) for funding my research (DOC ÖAW Fellowship\r\n26130, Horizon Europe BOLERO Project 101060393). ","department":[{"_id":"GradSch"},{"_id":"EvBe"}]},{"language":[{"iso":"eng"}],"related_material":{"record":[{"status":"public","id":"20465","relation":"earlier_version"}]},"das_tickbox":"1","day":"17","corr_author":"1","researchdata_availability":"yes","publication":"Nature Communications","_id":"22608","publisher":"Springer Nature","oa_version":"Published Version","article_processing_charge":"Yes","oa":1,"has_accepted_license":"1","quality_controlled":"1","article_number":"6499","PlanS_conform":"1","date_created":"2026-07-29T09:10:35Z","file":[{"date_updated":"2026-07-29T10:27:25Z","content_type":"application/pdf","success":1,"file_id":"22609","file_name":"2026_NatureComm_Naik.pdf","date_created":"2026-07-29T10:27:25Z","relation":"main_file","creator":"dernst","checksum":"f26d96e180c1d034d9c9c8f57c3c258b","access_level":"open_access","file_size":15363936}],"ddc":["570"],"OA_place":"publisher","article_type":"original","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","last_name":"Naik","orcid":"0000-0001-8421-5508","first_name":"Suyash","full_name":"Naik, Suyash"},{"last_name":"Keta","first_name":"Yann-Edwin","full_name":"Keta, Yann-Edwin"},{"full_name":"Pranjic-Ferscha, Kornelija","first_name":"Kornelija","last_name":"Pranjic-Ferscha","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo"},{"last_name":"Henkes","full_name":"Henkes, Silke","first_name":"Silke"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"}],"file_date_updated":"2026-07-29T10:27:25Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"date_updated":"2026-07-29T10:33:31Z","supplementarymaterial":"yes","publication_identifier":{"eissn":["2041-1723"]},"volume":17,"year":"2026","OA_type":"gold","month":"07","type":"journal_article","status":"public","date_published":"2026-07-17T00:00:00Z","title":"Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties","dataavailabilitystatement":"The authors declare that the minimum dataset that is necessary to\r\ninterpret, verify, and extend the research in this article is included in\r\nthe supplementary information, the source data, and the archived data\r\nrepository (https://doi.org/10.15479/AT-ISTA-21137). This is also available\r\non GitHub at https://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data. Source data are provided\r\nwith this paper. The framework to develop the vertex models used in this paper are\r\navailable online on GitHub and archived in the source data provided.\r\nCustom scripts used for analysis of imaging and simulation data are\r\nprovided along with data files for all panels in the source data for this\r\nmanuscript on GitHub and in data repo (https://doi.org/10.15479/ATISTA-\r\n21137). Framework for the vertex model is available at https://\r\ngithub.com/yketta/cells. Code for analysis is available on GitHub\r\nhttps://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data.","intvolume":"        17","citation":{"ieee":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P. J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026.","chicago":"Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo, Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-72366-z\">https://doi.org/10.1038/s41467-026-72366-z</a>.","ama":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-72366-z\">10.1038/s41467-026-72366-z</a>","ista":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. 2026. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. Nature Communications. 17, 6499.","apa":"Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &#38; Heisenberg, C.-P. J. (2026). Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-72366-z\">https://doi.org/10.1038/s41467-026-72366-z</a>","mla":"Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>Nature Communications</i>, vol. 17, 6499, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-72366-z\">10.1038/s41467-026-72366-z</a>.","short":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J. Heisenberg, Nature Communications 17 (2026)."},"publication_status":"published","external_id":{"pmid":["42143048"]},"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"abstract":[{"text":"For tissues to spread, they must deform while staying intact. How spreading tissues balance flexibility with integrity is not yet well understood. Here, we show that keratin intermediate filaments adapt tissue mechanical resilience to the stresses arising in epithelial tissues during spreading. By analyzing the expansion of the enveloping cell layer (EVL) over the yolk cell in zebrafish embryos in vivo, we find that keratin network maturation in EVL cells is promoted by stresses building up within the spreading tissue. Through genetic interference and tissue rheology experiments, complemented by a vertex model with mechanochemical feedback, we demonstrate that stress-induced keratin network maturation in the EVL increases tissue viscosity, to prevent tissue rupture. Further, keratins are required in the yolk cell for mechanosensitive actomyosin network contraction and flow, the forces pulling the EVL. These dual mechanosensitive functions of keratins enable a balance between pulling force production and EVL mechanical resilience, ensuring uniform and robust tissue spreading.","lang":"eng"}],"fulldoi":"https://doi.org/10.1038/s41467-026-72366-z","scopus_import":"1","doi":"10.1038/s41467-026-72366-z","department":[{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EdHa"}],"acknowledgement":"We thank all members of the Heisenberg, Henkes, and Hannezo groups for their support. We are also grateful to the Imaging and Optics, Scientific Computing, Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their technical assistance and support. Numerical simulations were performed using the computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020 research and innovation programme (grant agreement No. 665385). This work was supported by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to C.-P.H.","project":[{"name":"Keratins in epithelial tissue spreading","_id":"8f060199-16d5-11f0-9cad-f3253b266c46","grant_number":"PAT 5044023"},{"_id":"252C3B08-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"W1250-B20","name":"Nano-Analytics of Cellular Systems"}]}]