---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21040'
abstract:
- lang: eng
text: Formation during the first cycles of Li-rich layered oxide (LRLO) cathode
materials consolidates the interphase and leads to structural changes that are
decisive for long-term cyclability. However, the nature and effect of the changes
are material-dependent and unknown for the important class of Co-free, Ni-poor
LRLOs. Here, we analyze the processes during the tailored formation procedure
of a typical class member, Li1.28Ni0.15Mn0.57O2, and demonstrate that it remarkably
changes lattice composition and structure as a prerequisite for stable cycling.
We combine electrochemistry, operando mass spectrometry, X-ray diffraction, and
X-ray absorption spectroscopy with density functional theory simulations. Activation
most prominently compresses the layer spacing along the c-axis and increases reversible
structural breathing. The large capacity of ∼250 mAh g–1 originates from the Ni2+/Ni4+
and O2–/O– redox couples. Electron exchange during O-redox is smeared over the
entire anionic sublattice rather than localized on specific oxygen atomic sites.
This redox mechanism is reversible without detrimental oxygen evolution, avoiding
continued degradation common in conventional LRLOs. Sequential Ni- and O-redox
during activation irreversibly distorts the coordination of the redox-inactive
Mn4+ centers. This structural evolution of the MnO6 octahedra appears to enable
the superior electrochemical performance of this LRLO phase. These findings define
an activation pathway for the important class of Co-free, Ni-poor LRLOs, offering
potential guidance for the rational design of high-performance, more sustainable
cathode materials.
acknowledged_ssus:
- _id: M-Shop
- _id: LifeSc
acknowledgement: 'Elettra-Sincrotrone Trieste S.C.p.A. and its staff are acknowledged
for providing synchrotron radiation beamtime and laboratory facilities, in particular
the MCX and XAFS beamlines, where the XRD and XAS experiments have been carried
out, supported by the projects number: 20217082, 20205109, and 20195014. This study
was carried out within the MOST─Sustainable Mobility Center and received funding
from the European Union Next-Generation EU (PIANO NAZIONALE DI RIPRESA E RESILIENZA
(PNRR)─MISSIONE 4 COMPONENTE 2, INVESTIMENTO 1.4─D.D. 1033 17/06/2022, CN00000023).
Moreover, the contribution of S.B. and A.C. to this study was carried out within
the NEST─Network for Energy Sustainable Transition and received funding from the
European Union Next-Generation EU (PNRR─MISSIONE 4 COMPONENTE 2, INVESTIMENTO 1.3─D.D.
1561 11/10/2022, B53C22004070006). This manuscript reflects only the authors’ views
and opinions, neither the European Union nor the European Commission can be considered
responsible for them. Two of us, S.B. and S.A.F., would like to thank the Alistore
ERI. L.S. received funds from the Ministry of Ecological Transition in the “Ricerca
di Sistema Elettrico” framework. S.A.F. is indebted to ISTA for support. The Scientific
Service Units of ISTA supported this research through resources provided by the
Lab Support Facility and the Miba Machine Shop.'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Matteo
full_name: Busato, Matteo
last_name: Busato
- first_name: Mariarosaria
full_name: Tuccillo, Mariarosaria
last_name: Tuccillo
- first_name: Arcangelo
full_name: Celeste, Arcangelo
last_name: Celeste
- first_name: Alessandro
full_name: Tofoni, Alessandro
last_name: Tofoni
- first_name: Laura
full_name: Silvestri, Laura
last_name: Silvestri
- first_name: Paola
full_name: D’Angelo, Paola
last_name: D’Angelo
- first_name: Stefan Alexander
full_name: Freunberger, Stefan Alexander
id: A8CA28E6-CE23-11E9-AD2D-EC27E6697425
last_name: Freunberger
orcid: 0000-0003-2902-5319
- first_name: Sergio
full_name: Brutti, Sergio
last_name: Brutti
citation:
ama: Busato M, Tuccillo M, Celeste A, et al. Structural rearrangements of a Cobalt-free
Lithium-rich layered oxide cathode during formation. ACS Applied Energy Materials.
2026;9(1):686-697. doi:10.1021/acsaem.5c03511
apa: Busato, M., Tuccillo, M., Celeste, A., Tofoni, A., Silvestri, L., D’Angelo,
P., … Brutti, S. (2026). Structural rearrangements of a Cobalt-free Lithium-rich
layered oxide cathode during formation. ACS Applied Energy Materials. American
Chemical Society. https://doi.org/10.1021/acsaem.5c03511
chicago: Busato, Matteo, Mariarosaria Tuccillo, Arcangelo Celeste, Alessandro Tofoni,
Laura Silvestri, Paola D’Angelo, Stefan Alexander Freunberger, and Sergio Brutti.
“Structural Rearrangements of a Cobalt-Free Lithium-Rich Layered Oxide Cathode
during Formation.” ACS Applied Energy Materials. American Chemical Society,
2026. https://doi.org/10.1021/acsaem.5c03511.
ieee: M. Busato et al., “Structural rearrangements of a Cobalt-free Lithium-rich
layered oxide cathode during formation,” ACS Applied Energy Materials,
vol. 9, no. 1. American Chemical Society, pp. 686–697, 2026.
ista: Busato M, Tuccillo M, Celeste A, Tofoni A, Silvestri L, D’Angelo P, Freunberger
SA, Brutti S. 2026. Structural rearrangements of a Cobalt-free Lithium-rich layered
oxide cathode during formation. ACS Applied Energy Materials. 9(1), 686–697.
mla: Busato, Matteo, et al. “Structural Rearrangements of a Cobalt-Free Lithium-Rich
Layered Oxide Cathode during Formation.” ACS Applied Energy Materials,
vol. 9, no. 1, American Chemical Society, 2026, pp. 686–97, doi:10.1021/acsaem.5c03511.
short: M. Busato, M. Tuccillo, A. Celeste, A. Tofoni, L. Silvestri, P. D’Angelo,
S.A. Freunberger, S. Brutti, ACS Applied Energy Materials 9 (2026) 686–697.
corr_author: '1'
date_created: 2026-01-25T23:01:40Z
date_published: 2026-01-12T00:00:00Z
date_updated: 2026-02-12T14:04:04Z
day: '12'
ddc:
- '540'
department:
- _id: StFr
doi: 10.1021/acsaem.5c03511
file:
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checksum: 81272c19df41c696c1737168d3ea8c16
content_type: application/pdf
creator: dernst
date_created: 2026-02-12T13:55:28Z
date_updated: 2026-02-12T13:55:28Z
file_id: '21222'
file_name: 2026_AppliedEnergyMaterials_Busato.pdf
file_size: 5977526
relation: main_file
success: 1
file_date_updated: 2026-02-12T13:55:28Z
has_accepted_license: '1'
intvolume: ' 9'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 686-697
publication: ACS Applied Energy Materials
publication_identifier:
eissn:
- 2574-0962
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Structural rearrangements of a Cobalt-free Lithium-rich layered oxide cathode
during formation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2026'
...
---
_id: '21145'
abstract:
- lang: eng
text: 'Protein conformational energy landscapes are shaped not only by intramolecular
interactions but also by their environment. In protein crystals and protein-protein
complexes, intermolecular contacts alter this energy landscape, but the exact
nature of this alteration is difficult to decipher. Understanding how the crystal
lattice affects protein dynamics is crucial for crystallography-based studies
of motion, yet its influence on collective motions remains unclear. Aromatic ring
flips in the hydrophobic core represent sensitive probes of such dynamics. Here,
we compare the kinetics of aromatic ring flips in the protein GB1 in crystals,
in complex with its binding partner IgG, and in solution, combining advanced isotope
labeling with quantitative NMR methods. We show that rings in the core flip nearly
a thousand times less frequently in crystals than in solution. Enhanced-sampling
molecular dynamics simulations, based on a new crystal structure, reproduce these
elevated barriers and reveal how the crystal restrains motions. '
acknowledged_ssus:
- _id: NMR
- _id: LifeSc
acknowledgement: "We thank Nikolai R. Skrynnikov and Olga O. Lebedenko (St. Petersburg)
for insightful discussions and for performing exploratory MD simulations. We are
grateful to Tobias Schubeis (Lyon) for advice with GB1 crystallization, and Rebecca
Schmid for initial crystallization trials.\r\nWe thank Sebastian Falkner for assistance
with constructing the structural model of the IgG:GB1 complex.\r\nThis research
was supported by the Scientific Service Units (SSU) of Institute of Science and
Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance
and the Lab Support Facilities. We thank Petra Rovó and Margarita Valhondo Falcón
for excellent support of the NMR facility.\r\nLea M. Becker is recipient of a DOC
fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology
Austria (grant no. PR10660EAW01). Christophe Chipot acknowledges the European Research
Council (grant project 101097272 ``MilliInMicro'') and the Métropole du Grand Nancy
(grant project ``ARC''). BM07-FIP2 is supported by the French ANR PIA3 (France 2030)
EquipEx+ project MAGNIFIX under grant agreement ANR-21-ESRE-0011."
article_processing_charge: No
author:
- first_name: Lea Marie
full_name: Becker, Lea Marie
id: 36336939-eb97-11eb-a6c2-c83f1214ca79
last_name: Becker
orcid: 0000-0002-6401-5151
- first_name: Paul
full_name: Schanda, Paul
id: 7B541462-FAF6-11E9-A490-E8DFE5697425
last_name: Schanda
orcid: 0000-0002-9350-7606
- first_name: Christophe
full_name: Chipot, Christophe
last_name: Chipot
citation:
ama: Becker LM, Schanda P, Chipot C. Additional Data for “Aromatic Ring Flips Reveal
Reshaping of Protein Dynamics in Crystals and Complexes.” 2026. doi:10.15479/AT-ISTA-21145
apa: Becker, L. M., Schanda, P., & Chipot, C. (2026). Additional Data for “Aromatic
Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.” Institute
of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21145
chicago: Becker, Lea Marie, Paul Schanda, and Christophe Chipot. “Additional Data
for ‘Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and
Complexes.’” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21145.
ieee: L. M. Becker, P. Schanda, and C. Chipot, “Additional Data for ‘Aromatic Ring
Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.’” Institute
of Science and Technology Austria, 2026.
ista: Becker LM, Schanda P, Chipot C. 2026. Additional Data for ‘Aromatic Ring Flips
Reveal Reshaping of Protein Dynamics in Crystals and Complexes’, Institute of
Science and Technology Austria, 10.15479/AT-ISTA-21145.
mla: Becker, Lea Marie, et al. Additional Data for “Aromatic Ring Flips Reveal
Reshaping of Protein Dynamics in Crystals and Complexes.” Institute of Science
and Technology Austria, 2026, doi:10.15479/AT-ISTA-21145.
short: L.M. Becker, P. Schanda, C. Chipot, (2026).
contributor:
- contributor_type: researcher
first_name: Haohao
last_name: Fu
- contributor_type: researcher
first_name: Benjamin
id: 71cda2f3-e604-11ee-a1df-da10587eda3f
last_name: Tatman
- contributor_type: researcher
first_name: Matthias
last_name: Dreydoppel
- contributor_type: researcher
first_name: Anna
id: 9fb2a840-89e1-11ee-a8b7-cc5c7ba62471
last_name: Kapitonova
- contributor_type: researcher
first_name: Daniel
id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E
last_name: Balazs
orcid: 0000-0001-7597-043X
- contributor_type: researcher
first_name: Ulrich
last_name: Weininger
- contributor_type: researcher
first_name: Sylvain
last_name: Engilberge
corr_author: '1'
date_created: 2026-02-05T13:54:39Z
date_published: 2026-02-09T00:00:00Z
date_updated: 2026-02-18T10:04:44Z
day: '09'
ddc:
- '572'
department:
- _id: GradSch
- _id: PaSc
doi: 10.15479/AT-ISTA-21145
file:
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checksum: 02a419cce8cea450bc952f35488d2df5
content_type: text/plain
creator: lbecker
date_created: 2026-02-05T13:52:37Z
date_updated: 2026-02-05T13:52:37Z
file_id: '21146'
file_name: README.txt
file_size: 4263
relation: table_of_contents
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checksum: b0b82b1aa73985b0b308a3fa52d21aea
content_type: application/zip
creator: lbecker
date_created: 2026-02-05T13:52:41Z
date_updated: 2026-02-05T13:52:41Z
file_id: '21147'
file_name: Research_Data.zip
file_size: 50647107
relation: main_file
success: 1
file_date_updated: 2026-02-05T13:52:41Z
has_accepted_license: '1'
month: '02'
oa: 1
oa_version: Published Version
project:
- _id: 7be609c4-9f16-11ee-852c-85015ce2b9b0
grant_number: '26777'
name: Exploring protein dynamics by solid-state MAS NMR through specific labeling
approaches
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '20641'
relation: earlier_version
status: public
status: public
title: Additional Data for "Aromatic Ring Flips Reveal Reshaping of Protein Dynamics
in Crystals and Complexes"
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: repository
OA_type: free access
_id: '21284'
abstract:
- lang: eng
text: The advantageous characteristics attributed to the 19F nucleus have made it
a popular target for NMR once again in recent years. Aside from solution NMR,
an increasing number of studies have been conducted applying solid-state magic-angle-spinning
NMR to fluorine-labeled samples. Here, the high chemical shift anisotropy and
strong dipolar couplings can be utilized to get structural insights into proteins
and measure long distances. Despite increasing popularity and promising benefits,
the sensitivity of biomolecular 19F MAS NMR often suffers from slow longitudinal
T1 relaxation and therefore long recycle delays. In this work, we expand paramagnetic
doping, an approach commonly used to reduce proton T1 relaxation times, to 19F-labeled
biological samples. We study the effect of Gd(DTPA) and Gd(DTPA-BMA) on 19F and
13C T1 and T2 relaxation in a [5-19F13C]-tryptophan-labeled protein via 19F-detected
MAS NMR experiments. The observed paramagnetic relaxation enhancement substantially
reduces measurement times of 19F MAS NMR experiments without compromising resolution.
Additionally, we report the chemical-shift assignments of all four fluorotryptophan
signals in the 12 × 39 kDa large protein using a mutagenesis approach.
acknowledged_ssus:
- _id: NMR
- _id: LifeSc
acknowledgement: We thank Ben P. Tatman for insightful discussions. This research
was supported by the Scientific Service Units (SSU) of Institute of Science and
Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance
Facility and the Lab Support Facility.
article_processing_charge: No
author:
- first_name: Lea Marie
full_name: Becker, Lea Marie
id: 36336939-eb97-11eb-a6c2-c83f1214ca79
last_name: Becker
orcid: 0000-0002-6401-5151
- first_name: Paul
full_name: Schanda, Paul
id: 7B541462-FAF6-11E9-A490-E8DFE5697425
last_name: Schanda
orcid: 0000-0002-9350-7606
citation:
ama: Becker LM, Schanda P. Research data for “Accelerated 19F biomolecular magic-angle
spinning NMR with paramagnetic dopants.” 2026. doi:10.15479/AT-ISTA-21284
apa: Becker, L. M., & Schanda, P. (2026). Research data for “Accelerated 19F
biomolecular magic-angle spinning NMR with paramagnetic dopants.” Institute of
Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21284
chicago: Becker, Lea Marie, and Paul Schanda. “Research Data for ‘Accelerated 19F
Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.’” Institute of
Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21284.
ieee: L. M. Becker and P. Schanda, “Research data for ‘Accelerated 19F biomolecular
magic-angle spinning NMR with paramagnetic dopants.’” Institute of Science and
Technology Austria, 2026.
ista: Becker LM, Schanda P. 2026. Research data for ‘Accelerated 19F biomolecular
magic-angle spinning NMR with paramagnetic dopants’, Institute of Science and
Technology Austria, 10.15479/AT-ISTA-21284.
mla: Becker, Lea Marie, and Paul Schanda. Research Data for “Accelerated 19F
Biomolecular Magic-Angle Spinning NMR with Paramagnetic Dopants.” Institute
of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-21284.
short: L.M. Becker, P. Schanda, (2026).
contributor:
- contributor_type: researcher
first_name: Giorgia
id: 334a5e40-8747-11f0-b671-ba1f5154b4b4
last_name: Toscano
- contributor_type: researcher
first_name: Anna
id: 9fb2a840-89e1-11ee-a8b7-cc5c7ba62471
last_name: Kapitonova
- contributor_type: researcher
first_name: Rajkumar
id: a3089acd-6806-11ee-bacc-f0c7d500ad20
last_name: Singh
- contributor_type: researcher
first_name: Undina
id: bb74f472-ae54-11eb-9835-bc9c22fb1183
last_name: Guillerm
- contributor_type: researcher
first_name: Roman
last_name: Lichtenecker
corr_author: '1'
date_created: 2026-02-17T10:17:14Z
date_published: 2026-02-18T00:00:00Z
date_updated: 2026-02-18T10:12:49Z
day: '18'
ddc:
- '541'
department:
- _id: GradSch
- _id: PaSc
doi: 10.15479/AT-ISTA-21284
file:
- access_level: open_access
checksum: 2d3105f26be578073b88ee1f2ea0bdb1
content_type: application/zip
creator: lbecker
date_created: 2026-02-17T10:11:14Z
date_updated: 2026-02-17T10:11:14Z
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file_name: Research_data.zip
file_size: 36996027
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creator: lbecker
date_created: 2026-02-17T10:11:14Z
date_updated: 2026-02-17T10:11:14Z
file_id: '21286'
file_name: README.txt
file_size: 1993
relation: table_of_contents
file_date_updated: 2026-02-17T10:11:14Z
has_accepted_license: '1'
month: '2'
oa: 1
oa_version: None
publisher: Institute of Science and Technology Austria
status: public
title: Research data for "Accelerated 19F biomolecular magic-angle spinning NMR with
paramagnetic dopants"
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: research_data
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2026'
...
---
OA_place: repository
_id: '21360'
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: "I would like to acknowledge the Austrian Academy of Sciences (ÖAW)
and European\r\nResearch Executive Agency (REA) for funding my research (DOC ÖAW
Fellowship\r\n26130, Horizon Europe BOLERO Project 101060393). "
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Stefan
full_name: Riegler, Stefan
id: FF6018E0-D806-11E9-8E43-0B14E6697425
last_name: Riegler
orcid: 0000-0003-3413-1343
citation:
ama: 'Riegler S. Root system plasticity under nutrient limitation : Investigating
hormonal and molecular drivers in Arabidopsis thaliana and Coffea species. 2026.
doi:10.15479/AT-ISTA-21360'
apa: 'Riegler, S. (2026). Root system plasticity under nutrient limitation :
Investigating hormonal and molecular drivers in Arabidopsis thaliana and Coffea
species. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21360'
chicago: 'Riegler, Stefan. “Root System Plasticity under Nutrient Limitation : Investigating
Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea Species.” Institute
of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21360.'
ieee: 'S. Riegler, “Root system plasticity under nutrient limitation : Investigating
hormonal and molecular drivers in Arabidopsis thaliana and Coffea species,” Institute
of Science and Technology Austria, 2026.'
ista: 'Riegler S. 2026. Root system plasticity under nutrient limitation : Investigating
hormonal and molecular drivers in Arabidopsis thaliana and Coffea species. Institute
of Science and Technology Austria.'
mla: 'Riegler, Stefan. Root System Plasticity under Nutrient Limitation : Investigating
Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea Species.
Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-21360.'
short: 'S. Riegler, Root System Plasticity under Nutrient Limitation : Investigating
Hormonal and Molecular Drivers in Arabidopsis Thaliana and Coffea Species, Institute
of Science and Technology Austria, 2026.'
corr_author: '1'
date_created: 2026-02-27T09:08:14Z
date_published: 2026-02-26T00:00:00Z
date_updated: 2026-03-09T12:20:56Z
day: '26'
ddc:
- '570'
- '575'
- '583'
degree_awarded: PhD
department:
- _id: GradSch
- _id: EvBe
doi: 10.15479/AT-ISTA-21360
file:
- access_level: closed
checksum: 2f1f44e8536c2538f94a440217452c9f
content_type: application/x-zip-compressed
creator: sriegler
date_created: 2026-03-02T10:59:50Z
date_updated: 2026-03-02T10:59:50Z
file_id: '21386'
file_name: 2026_Riegler_Stefan_Thesis.zip
file_size: 31430022
relation: source_file
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content_type: application/pdf
creator: sriegler
date_created: 2026-03-02T10:59:49Z
date_updated: 2026-03-02T10:59:49Z
embargo: 2027-02-27
embargo_to: open_access
file_id: '21387'
file_name: 2026_Riegler_Stefan_Thesis.pdf
file_size: 11635090
relation: main_file
file_date_updated: 2026-03-02T10:59:50Z
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-sa/4.0/
month: '02'
oa_version: Published Version
page: '185'
project:
- _id: 34afa094-11ca-11ed-8bc3-a375845a59fb
grant_number: '101060393'
name: Breeding for coffee and cocoa root resilience in low input farming systems
based on improved rootstocks
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '21363'
relation: research_data
status: public
status: public
supervisor:
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
title: 'Root system plasticity under nutrient limitation : Investigating hormonal
and molecular drivers in Arabidopsis thaliana and Coffea species'
tmp:
image: /images/cc_by_sa.png
legal_code_url: https://creativecommons.org/licenses/by-sa/4.0/legalcode
name: Creative Commons Attribution-ShareAlike 4.0 International Public License (CC
BY-SA 4.0)
short: CC BY-SA (4.0)
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21015'
abstract:
- lang: eng
text: Early embryo geometry is one of the most invariant species-specific traits,
yet its role in ensuring developmental reproducibility and robustness remains
underexplored. Here we show that in zebrafish, the geometry of the fertilized
egg—specifically its curvature and volume—serves as a critical initial condition
triggering a cascade of events that influence development. The embryo geometry
guides patterned asymmetric cell divisions in the blastoderm, generating radial
gradients of cell volume and nucleocytoplasmic ratio. These gradients generate
mitotic phase waves, with the nucleocytoplasmic ratio determining individual cell
cycle periods independently of other cells. We demonstrate that reducing cell
autonomy reshapes these waves, emphasizing the instructive role of geometry-derived
volume patterns in setting the intrinsic period of the cell cycle oscillator.
In addition to organizing cell cycles, early embryo geometry spatially patterns
zygotic genome activation at the midblastula transition, a key step in establishing
embryonic autonomy. Disrupting the embryo shape alters the zygotic genome activation
pattern and causes ectopic germ layer specification, underscoring the developmental
significance of geometry. Together, our findings reveal a symmetry-breaking function
of early embryo geometry in coordinating cell cycle and transcriptional patterning.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
- _id: ScienComp
- _id: LifeSc
acknowledgement: We thank N. Petridou (EMBL) for sharing results before publication.
N.M. was supported by funding from the European Union’s Horizon 2020 programme under
the Marie Skłodowska-Curie COFUND Actions ISTplus grant agreement number 754411.
Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and
innovation programme under the Marie Skłodowska-Curie grant agreement number 101034413.
The research was supported by funding to C.-P.H. from the NOMIS Foundation, Project
ID 1.844. We would like to thank past and present members of the Heisenberg and
Hannezo groups for discussions, particularly S. Shamipour, V. Doddihal, M. Jovic,
N. Hino, F. N. Arslan, R. Kobylinska and C. Camelo for feedback on the draft manuscript.
This research was supported by the Scientific Service Units (SSU) of Institute of
Science and Technology Austria through resources provided by the Aquatics Facility,
Imaging & Optics Facility (IOF), Scientific Computing (SciComp) facility and Lab
Support Facility (LSF). Open access funding provided by Institute of Science and
Technology (IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Nikhil
full_name: Mishra, Nikhil
id: C4D70E82-1081-11EA-B3ED-9A4C3DDC885E
last_name: Mishra
orcid: 0000-0002-6425-5788
- first_name: Yuting I
full_name: Li, Yuting I
id: ee7a5ca8-8b71-11ed-b662-b3341c05b7eb
last_name: Li
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. Geometry-driven asymmetric cell
divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo.
Nature Physics. 2026;22:139-150. doi:10.1038/s41567-025-03122-1
apa: Mishra, N., Li, Y. I., Hannezo, E. B., & Heisenberg, C.-P. J. (2026). Geometry-driven
asymmetric cell divisions pattern cell cycles and zygotic genome activation in
the zebrafish embryo. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-025-03122-1
chicago: Mishra, Nikhil, Yuting I Li, Edouard B Hannezo, and Carl-Philipp J Heisenberg.
“Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome
Activation in the Zebrafish Embryo.” Nature Physics. Springer Nature, 2026.
https://doi.org/10.1038/s41567-025-03122-1.
ieee: N. Mishra, Y. I. Li, E. B. Hannezo, and C.-P. J. Heisenberg, “Geometry-driven
asymmetric cell divisions pattern cell cycles and zygotic genome activation in
the zebrafish embryo,” Nature Physics, vol. 22. Springer Nature, pp. 139–150,
2026.
ista: Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. 2026. Geometry-driven asymmetric
cell divisions pattern cell cycles and zygotic genome activation in the zebrafish
embryo. Nature Physics. 22, 139–150.
mla: Mishra, Nikhil, et al. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell
Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” Nature Physics,
vol. 22, Springer Nature, 2026, pp. 139–50, doi:10.1038/s41567-025-03122-1.
short: N. Mishra, Y.I. Li, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 22 (2026)
139–150.
corr_author: '1'
date_created: 2026-01-20T10:12:19Z
date_published: 2026-01-05T00:00:00Z
date_updated: 2026-03-16T13:18:36Z
day: '05'
ddc:
- '570'
department:
- _id: EdHa
- _id: CaHe
doi: 10.1038/s41567-025-03122-1
ec_funded: 1
external_id:
oaworkid:
- W7118187193
file:
- access_level: open_access
checksum: 0ab7ac2fbcb61a364dba57152db64ed7
content_type: application/pdf
creator: dernst
date_created: 2026-01-21T08:21:11Z
date_updated: 2026-01-21T08:21:11Z
file_id: '21026'
file_name: 2026_NaturePhysics_Mishra.pdf
file_size: 7335694
relation: main_file
success: 1
file_date_updated: 2026-01-21T08:21:11Z
has_accepted_license: '1'
intvolume: ' 22'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
oaworkid: 1
page: 139-150
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
call_identifier: H2020
grant_number: '101034413'
name: 'IST-BRIDGE: International postdoctoral program'
- _id: 917c023a-16d5-11f0-9cad-eb5cafc52090
name: Cytoplasmic self-organization into cell-like compartments as a common guiding
principle in early animal development
publication: Nature Physics
publication_identifier:
eissn:
- 1745-2481
issn:
- 1745-2473
issnl:
- ' 1745-2473'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome
activation in the zebrafish embryo
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 22
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21485'
abstract:
- lang: eng
text: Insulating oxides are among the most abundant solid materials in the universe1,2,3.
Of the many ways in which they influence natural phenomena, perhaps the most consequential
is their capacity to transfer electrical charge during contact4,5,6,7,8,9,10—which
occurs even between samples of the same oxide—yet the symmetry-breaking parameter
that causes this remains unidentified11,12. Here we show that adventitious carbonaceous
molecules adsorbed from the environment are the symmetry-breaking factor in same-material
oxide contact electrification (CE). We use acoustic levitation to measure charge
exchange between a sphere and a plate composed of identical amorphous silicon
dioxide (SiO2). Although charging polarity is random for co-prepared samples,
we control it with baking or plasma treatment. Observing the charge-exchange relaxation
afterwards, we see dynamics over a timescale of hours and connect this directly
to the presence of adventitious carbon with time-of-flight mass spectrometry,
low-energy ion scattering and infrared spectroscopy. Going further, we confirm
that adventitious carbon can even determine charge exchange among different oxides.
Our results identify the symmetry-breaking parameter that causes insulating oxides
to exchange charge in settings ranging from desert sands4 to volcanic plumes5,6,
while simultaneously highlighting an overlooked factor in CE more broadly.
acknowledged_ssus:
- _id: M-Shop
- _id: NanoFab
- _id: ScienComp
- _id: LifeSc
acknowledgement: This project has received support from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation programme
(grant agreement no. 949120) and from the Marie Skłodowska-Curie programme (grant
agreement no. 754411). We acknowledge the state of Lower Austria and the European
Regional Development Fund under grant no. WST3-F-542638/004-2021. N.M. acknowledges
support from grant Fondecyt 1221597. G.G. is a Serra Húnter fellow. This research
was supported by the Scientific Service Units of the Institute of Science and Technology
Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility,
Scientific Computing facility and Lab Support Facility. We thank the Modic group
for the use of the Laue camera, T. Zauner for the photography of the experimental
set-up and R. Möller for insightful discussions. Open access funding provided by
Institute of Science and Technology (IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Galien M
full_name: Grosjean, Galien M
id: 0C5FDA4A-9CF6-11E9-8939-FF05E6697425
last_name: Grosjean
orcid: 0000-0001-5154-417X
- first_name: Markus
full_name: Ostermann, Markus
last_name: Ostermann
- first_name: Markus
full_name: Sauer, Markus
last_name: Sauer
- first_name: Michael
full_name: Hahn, Michael
last_name: Hahn
- first_name: Christian M.
full_name: Pichler, Christian M.
last_name: Pichler
- first_name: Florian
full_name: Fahrnberger, Florian
last_name: Fahrnberger
- first_name: Felix
full_name: Pertl, Felix
id: 6313aec0-15b2-11ec-abd3-ed67d16139af
last_name: Pertl
orcid: 0000-0003-0463-5794
- first_name: Daniel
full_name: Balazs, Daniel
id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E
last_name: Balazs
orcid: 0000-0001-7597-043X
- first_name: Mason M.
full_name: Link, Mason M.
last_name: Link
- first_name: Seong H.
full_name: Kim, Seong H.
last_name: Kim
- first_name: Devin L.
full_name: Schrader, Devin L.
last_name: Schrader
- first_name: Adriana
full_name: Blanco, Adriana
last_name: Blanco
- first_name: Francisco
full_name: Gracia, Francisco
last_name: Gracia
- first_name: Nicolás
full_name: Mujica, Nicolás
last_name: Mujica
- first_name: Scott R
full_name: Waitukaitis, Scott R
id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87
last_name: Waitukaitis
orcid: 0000-0002-2299-3176
citation:
ama: Grosjean GM, Ostermann M, Sauer M, et al. Adventitious carbon breaks symmetry
in oxide contact electrification. Nature. 2026;651(8106):626-631. doi:10.1038/s41586-025-10088-w
apa: Grosjean, G. M., Ostermann, M., Sauer, M., Hahn, M., Pichler, C. M., Fahrnberger,
F., … Waitukaitis, S. R. (2026). Adventitious carbon breaks symmetry in oxide
contact electrification. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-10088-w
chicago: Grosjean, Galien M, Markus Ostermann, Markus Sauer, Michael Hahn, Christian
M. Pichler, Florian Fahrnberger, Felix Pertl, et al. “Adventitious Carbon Breaks
Symmetry in Oxide Contact Electrification.” Nature. Springer Nature, 2026.
https://doi.org/10.1038/s41586-025-10088-w.
ieee: G. M. Grosjean et al., “Adventitious carbon breaks symmetry in oxide
contact electrification,” Nature, vol. 651, no. 8106. Springer Nature,
pp. 626–631, 2026.
ista: Grosjean GM, Ostermann M, Sauer M, Hahn M, Pichler CM, Fahrnberger F, Pertl
F, Balazs D, Link MM, Kim SH, Schrader DL, Blanco A, Gracia F, Mujica N, Waitukaitis
SR. 2026. Adventitious carbon breaks symmetry in oxide contact electrification.
Nature. 651(8106), 626–631.
mla: Grosjean, Galien M., et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact
Electrification.” Nature, vol. 651, no. 8106, Springer Nature, 2026, pp.
626–31, doi:10.1038/s41586-025-10088-w.
short: G.M. Grosjean, M. Ostermann, M. Sauer, M. Hahn, C.M. Pichler, F. Fahrnberger,
F. Pertl, D. Balazs, M.M. Link, S.H. Kim, D.L. Schrader, A. Blanco, F. Gracia,
N. Mujica, S.R. Waitukaitis, Nature 651 (2026) 626–631.
corr_author: '1'
date_created: 2026-03-23T15:04:00Z
date_published: 2026-03-18T00:00:00Z
date_updated: 2026-03-24T06:59:57Z
day: '18'
ddc:
- '540'
department:
- _id: ScWa
- _id: GradSch
- _id: LifeSc
doi: 10.1038/s41586-025-10088-w
ec_funded: 1
external_id:
pmid:
- '41851325'
file:
- access_level: open_access
checksum: dafef9ed575b44be4263e948a47ae056
content_type: application/pdf
creator: dernst
date_created: 2026-03-24T06:57:08Z
date_updated: 2026-03-24T06:57:08Z
file_id: '21494'
file_name: 2026_Nature_Grosjean.pdf
file_size: 12245694
relation: main_file
success: 1
file_date_updated: 2026-03-24T06:57:08Z
has_accepted_license: '1'
intvolume: ' 651'
issue: '8106'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 626-631
pmid: 1
project:
- _id: 0aa60e99-070f-11eb-9043-a6de6bdc3afa
call_identifier: H2020
grant_number: '949120'
name: 'Tribocharge: a multi-scale approach to an enduring problem in physics'
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: Adventitious carbon breaks symmetry in oxide contact electrification
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 651
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21490'
abstract:
- lang: eng
text: Auxin canalization is a self-organizing process that governs the flexible
formation of vasculature by reinforcing the formation of auxin transport channels.
A key prerequisite is the feedback between auxin signaling and directional auxin
transport, mediated by PIN transporters. Despite the developmental importance
of canalization, the molecular components linking auxin perception to the regulation
of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel
and essential component of auxin canalization that links intracellular auxin signaling
with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23
transcriptional auxin signaling. tow mutants exhibit defects in regeneration and
de novo vasculature formation, along with impaired formation of polarized, PIN-expressing
auxin channels. At the subcellular level, these mutants display disrupted auxin-induced
PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes
predominantly to the plasma membrane, where it interacts with receptor-like kinases
involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR
complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at
the cell surface. Together, our findings identify TOW as a molecular link between
intracellular and cell surface auxin signaling mechanisms that converge on PIN
trafficking and polarity, providing new insights into how auxin signaling regulates
directional auxin transport for the self-organizing formation of vasculature during
flexible plant development.
acknowledged_ssus:
- _id: MassSpec
- _id: Bio
- _id: LifeSc
acknowledgement: We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9
system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof.
Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions,
and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also
acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging
& Optics Facility, and the Lab Support Facility at the Institute of Science and
Technology Austria. This research was supported by the Scientific Service Units
(SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF)
and the Lab Support Facility (LSF). The work conducted by the Friml group was funded
by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS)
and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the
core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant
Sciences Core Facility of CEITEC Masaryk University. E.M. received support from
the National Science Centre (NCN), Poland, through the OPUS call within the Weave
programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next
GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes
Amos Comenius.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Mingyue
full_name: Li, Mingyue
id: 01f96916-0235-11eb-9379-a323192643b7
last_name: Li
- first_name: Nikola
full_name: Rydza, Nikola
last_name: Rydza
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting
protein TOW stabilizes PIN transporters for auxin canalization. Current Biology.
2026;36(6):1468-1480.e6. doi:10.1016/j.cub.2026.02.023
apa: Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., & Friml, J. (2026).
Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin
canalization. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2026.02.023
chicago: Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński,
and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters
for Auxin Canalization.” Current Biology. Elsevier, 2026. https://doi.org/10.1016/j.cub.2026.02.023.
ieee: M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting
protein TOW stabilizes PIN transporters for auxin canalization,” Current Biology,
vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026.
ista: Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting
protein TOW stabilizes PIN transporters for auxin canalization. Current Biology.
36(6), 1468–1480.e6.
mla: Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes
PIN Transporters for Auxin Canalization.” Current Biology, vol. 36, no.
6, Elsevier, 2026, p. 1468–1480.e6, doi:10.1016/j.cub.2026.02.023.
short: M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology
36 (2026) 1468–1480.e6.
corr_author: '1'
date_created: 2026-03-23T15:11:16Z
date_published: 2026-03-23T00:00:00Z
date_updated: 2026-03-24T08:36:40Z
day: '23'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.cub.2026.02.023
external_id:
pmid:
- '41831441'
file:
- access_level: open_access
checksum: fe6c41fdab58a55df5f2a5860c02acdc
content_type: application/pdf
creator: dernst
date_created: 2026-03-24T08:34:37Z
date_updated: 2026-03-24T08:34:37Z
file_id: '21496'
file_name: 2026_CurrentBiology_Li.pdf
file_size: 12986894
relation: main_file
success: 1
file_date_updated: 2026-03-24T08:34:37Z
has_accepted_license: '1'
intvolume: ' 36'
issue: '6'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 1468-1480.e6
pmid: 1
project:
- _id: 8f347782-16d5-11f0-9cad-8c19706ee739
grant_number: '101142681'
name: Cyclic nucleotides as second messengers in plants
- _id: bd906599-d553-11ed-ba76-abf8547645d7
grant_number: E271
name: Identification of a novel regulator in auxin canalization
publication: Current Biology
publication_identifier:
issn:
- 0960-9822
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for
auxin canalization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2026'
...
---
OA_place: repository
OA_type: free access
_id: '21137'
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: ScienComp
- _id: LifeSc
acknowledgement: We thank all members of the Heisenberg, Henkes, and Hannezo groups
for their support. We are also grateful to the Imaging and Optics, Scientific Computing,
Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their
technical assistance and support. Numerical simulations were performed using the
computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary
Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided
by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020
research and innovation programme (grant agreement No. 665385). This work was supported
by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to
C.-P.H.
article_processing_charge: No
author:
- first_name: Suyash
full_name: Naik, Suyash
id: 2C0B105C-F248-11E8-B48F-1D18A9856A87
last_name: Naik
orcid: 0000-0001-8421-5508
citation:
ama: Naik S. Data associated with Keratins coordinate tissue spreading . 2026. doi:10.15479/AT-ISTA-21137
apa: Naik, S. (2026). Data associated with Keratins coordinate tissue spreading
. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21137
chicago: Naik, Suyash. “Data Associated with Keratins Coordinate Tissue Spreading
.” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21137.
ieee: S. Naik, “Data associated with Keratins coordinate tissue spreading .” Institute
of Science and Technology Austria, 2026.
ista: Naik S. 2026. Data associated with Keratins coordinate tissue spreading ,
Institute of Science and Technology Austria, 10.15479/AT-ISTA-21137.
mla: Naik, Suyash. Data Associated with Keratins Coordinate Tissue Spreading
. Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-21137.
short: S. Naik, (2026).
contributor:
- contributor_type: researcher
first_name: Yann-Edwin
last_name: Keta
- contributor_type: supervisor
first_name: 'Silke '
last_name: Henkes
- contributor_type: supervisor
first_name: Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- contributor_type: supervisor
first_name: Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
corr_author: '1'
date_created: 2026-02-04T16:38:02Z
date_published: 2026-03-24T00:00:00Z
date_updated: 2026-03-24T08:32:00Z
day: '24'
department:
- _id: GradSch
- _id: CaHe
- _id: EdHa
doi: 10.15479/AT-ISTA-21137
ec_funded: 1
file:
- access_level: open_access
checksum: 5d1fda7e410f24c311fcf6bcf725698f
content_type: application/zip
creator: snaik
date_created: 2026-03-16T11:51:10Z
date_updated: 2026-03-16T11:51:10Z
description: 'Python3 library written in C++20 to integrate vertex models. Please
read the readme at https://github.com/yketa/cells/blob/main/README.md for detailed
instructions for installation and usage of the code in this repository. '
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month: '3'
oa: 1
oa_version: None
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 8f060199-16d5-11f0-9cad-f3253b266c46
grant_number: PAT 5044023
name: Keratins in epithelial tissue spreading
- _id: 252C3B08-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1250-B20
name: Nano-Analytics of Cellular Systems
publisher: Institute of Science and Technology Austria
status: public
title: 'Data associated with Keratins coordinate tissue spreading '
tmp:
image: /images/cc_by_sa.png
legal_code_url: https://creativecommons.org/licenses/by-sa/4.0/legalcode
name: Creative Commons Attribution-ShareAlike 4.0 International Public License (CC
BY-SA 4.0)
short: CC BY-SA (4.0)
type: research_data
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2026'
...
---
OA_place: publisher
_id: '20964'
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
alternative_title:
- ISTA Master’s Thesis
article_processing_charge: No
author:
- first_name: Dmitrii
full_name: Vladimirtsev, Dmitrii
id: 60466724-5355-11ee-ae5a-fa55e8f99c3d
last_name: Vladimirtsev
citation:
ama: Vladimirtsev D. Armadillo repeat only proteins are master regulators of plant
cyclic-nucleotide gated channels. 2026. doi:10.15479/AT-ISTA-20964
apa: Vladimirtsev, D. (2026). Armadillo repeat only proteins are master regulators
of plant cyclic-nucleotide gated channels. Institute of Science and Technology
Austria. https://doi.org/10.15479/AT-ISTA-20964
chicago: Vladimirtsev, Dmitrii. “Armadillo Repeat Only Proteins Are Master Regulators
of Plant Cyclic-Nucleotide Gated Channels.” Institute of Science and Technology
Austria, 2026. https://doi.org/10.15479/AT-ISTA-20964.
ieee: D. Vladimirtsev, “Armadillo repeat only proteins are master regulators of
plant cyclic-nucleotide gated channels,” Institute of Science and Technology Austria,
2026.
ista: Vladimirtsev D. 2026. Armadillo repeat only proteins are master regulators
of plant cyclic-nucleotide gated channels. Institute of Science and Technology
Austria.
mla: Vladimirtsev, Dmitrii. Armadillo Repeat Only Proteins Are Master Regulators
of Plant Cyclic-Nucleotide Gated Channels. Institute of Science and Technology
Austria, 2026, doi:10.15479/AT-ISTA-20964.
short: D. Vladimirtsev, Armadillo Repeat Only Proteins Are Master Regulators of
Plant Cyclic-Nucleotide Gated Channels, Institute of Science and Technology Austria,
2026.
corr_author: '1'
date_created: 2026-01-09T09:22:48Z
date_published: 2026-01-14T00:00:00Z
date_updated: 2026-04-07T11:41:44Z
day: '14'
ddc:
- '570'
degree_awarded: MS
department:
- _id: GradSch
- _id: JiFr
doi: 10.15479/AT-ISTA-20964
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date_updated: 2026-01-21T14:12:13Z
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month: '01'
oa_version: Published Version
page: '22'
project:
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grant_number: '101142681'
name: Cyclic nucleotides as second messengers in plants
publication_identifier:
issn:
- 2791-4585
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
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relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide
gated channels
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2026'
...
---
OA_type: closed access
_id: '21730'
abstract:
- lang: eng
text: Hydrogen peroxide (H2O2) is a crucial member of the reactive oxygen species
(ROS) family, playing roles in cellular signalling and immune responses in human
health. Moreover, it is a potential biomarker of diabetes when present in aberrant
concentrations. Therefore, monitoring trace levels of H2O2 has become a research
hotspot for analytical and sensor chemists. In this context, we report a rhodamine-based
fluorescent probe (RN), which shows excellent fluorescent enhancement at 555 nm
upon the addition of H2O2 along with a low limit of detection (LOD) of 0.67 ppm
and fast response (∼2 min). The probe is highly selective for H2O2, showing no
fluorescence enhancement with other ROS. RN is synthesised in a one-pot chemical
reaction using rhodamine 6G (R6G) and 4,7,10-trioxa-1,13-tridecanediamine (TTDA).
H2O2 detection in pre-treated milk samples proves its real-world viability. We
found that RN shows low cytotoxicity, which allowed us to successfully explore
its potential to monitor H2O2 generation in a diabetic L929 skin cell line and
diabetic mice liver tissue. This result demonstrates promising features for assessing
early diabetic progression through fluorescence imaging.
acknowledged_ssus:
- _id: LifeSc
acknowledgement: "MM acknowledges the Government of India for DST-INSPIRE\r\nfellowship
[IF200389] and Federal Ministry of Education, Science and Research (BMBWF) and the
OeAD – Austria’s Agency for Education and Internationalisation for an Ernst Mach
Grant, weltweit (grant number MPC-2024-01518) for research internship at ISTA. The
Scientific Service Units of ISTA supported this research through resources provided
by the Lab Support Facility. PG acknowledges the ANRF, India, for his NPDF fellowship
(File no. PDF/2022/001960). PB acknowledges ANRF, India, for the SERB-CRG sponsored
project GAP-240712 (vide reference no. CRG/2022/001679)."
article_processing_charge: No
article_type: original
author:
- first_name: Moumita
full_name: Mondal, Moumita
last_name: Mondal
- first_name: Pravat
full_name: Ghorai, Pravat
last_name: Ghorai
- first_name: Asmita
full_name: Samadder, Asmita
last_name: Samadder
- first_name: Stefan Alexander
full_name: Freunberger, Stefan Alexander
id: A8CA28E6-CE23-11E9-AD2D-EC27E6697425
last_name: Freunberger
orcid: 0000-0003-2902-5319
- first_name: Priyabrata
full_name: Banerjee, Priyabrata
last_name: Banerjee
citation:
ama: Mondal M, Ghorai P, Samadder A, Freunberger SA, Banerjee P. H2O2 responsive
rhodamine-based probe for monitoring early-stage diabetes diagnosis. Journal
of Materials Chemistry B. 2026. doi:10.1039/d5tb02687c
apa: Mondal, M., Ghorai, P., Samadder, A., Freunberger, S. A., & Banerjee, P.
(2026). H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes
diagnosis. Journal of Materials Chemistry B. Royal Society of Chemistry.
https://doi.org/10.1039/d5tb02687c
chicago: Mondal, Moumita, Pravat Ghorai, Asmita Samadder, Stefan Alexander Freunberger,
and Priyabrata Banerjee. “H2O2 Responsive Rhodamine-Based Probe for Monitoring
Early-Stage Diabetes Diagnosis.” Journal of Materials Chemistry B. Royal
Society of Chemistry, 2026. https://doi.org/10.1039/d5tb02687c.
ieee: M. Mondal, P. Ghorai, A. Samadder, S. A. Freunberger, and P. Banerjee, “H2O2
responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis,”
Journal of Materials Chemistry B. Royal Society of Chemistry, 2026.
ista: Mondal M, Ghorai P, Samadder A, Freunberger SA, Banerjee P. 2026. H2O2 responsive
rhodamine-based probe for monitoring early-stage diabetes diagnosis. Journal of
Materials Chemistry B.
mla: Mondal, Moumita, et al. “H2O2 Responsive Rhodamine-Based Probe for Monitoring
Early-Stage Diabetes Diagnosis.” Journal of Materials Chemistry B, Royal
Society of Chemistry, 2026, doi:10.1039/d5tb02687c.
short: M. Mondal, P. Ghorai, A. Samadder, S.A. Freunberger, P. Banerjee, Journal
of Materials Chemistry B (2026).
corr_author: '1'
date_created: 2026-04-13T07:45:26Z
date_published: 2026-04-10T00:00:00Z
date_updated: 2026-04-16T05:44:49Z
day: '10'
department:
- _id: StFr
doi: 10.1039/d5tb02687c
external_id:
pmid:
- '41958432'
language:
- iso: eng
month: '04'
oa_version: None
pmid: 1
publication: Journal of Materials Chemistry B
publication_identifier:
eissn:
- 2050-7518
issn:
- 2050-750X
publication_status: epub_ahead
publisher: Royal Society of Chemistry
quality_controlled: '1'
scopus_import: '1'
status: public
title: H2O2 responsive rhodamine-based probe for monitoring early-stage diabetes diagnosis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: repository
OA_type: gold
_id: '18697'
abstract:
- lang: eng
text: The information-processing capability of the brain’s cellular network depends
on the physical wiring pattern between neurons and their molecular and functional
characteristics. Mapping neurons and resolving their individual synaptic connections
can be achieved by volumetric imaging at nanoscale resolution with dense cellular
labelling. Light microscopy is uniquely positioned to visualize specific molecules
but dense, synapse-level circuit reconstruction by light microscopy has been out
of reach due to limitations in resolution, contrast, and volumetric imaging capability.
Here we developed light-microscopy based connectomics (LICONN). We integrated
specifically engineered hydrogel embedding and expansion with comprehensive deep-learning
based segmentation and analysis of connectivity, thus directly incorporating molecular
information in synapse-level brain tissue reconstructions. LICONN will allow synapse-level
brain tissue phenotyping in biological experiments in a readily adoptable manner.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: ScienComp
- _id: PreCl
- _id: M-Shop
- _id: E-Lib
article_processing_charge: No
author:
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
- first_name: Julia
full_name: Lyudchik, Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- first_name: Caroline
full_name: Kreuzinger, Caroline
id: 382077BA-F248-11E8-B48F-1D18A9856A87
last_name: Kreuzinger
citation:
ama: Danzl JG, Lyudchik J, Kreuzinger C. Light-microscopy based connectomic reconstruction
of mammalian brain tissue. 2025. doi:10.15479/AT:ISTA:18697
apa: Danzl, J. G., Lyudchik, J., & Kreuzinger, C. (2025). Light-microscopy based
connectomic reconstruction of mammalian brain tissue. Institute of Science and
Technology Austria. https://doi.org/10.15479/AT:ISTA:18697
chicago: Danzl, Johann G, Julia Lyudchik, and Caroline Kreuzinger. “Light-Microscopy
Based Connectomic Reconstruction of Mammalian Brain Tissue.” Institute of Science
and Technology Austria, 2025. https://doi.org/10.15479/AT:ISTA:18697.
ieee: J. G. Danzl, J. Lyudchik, and C. Kreuzinger, “Light-microscopy based connectomic
reconstruction of mammalian brain tissue.” Institute of Science and Technology
Austria, 2025.
ista: Danzl JG, Lyudchik J, Kreuzinger C. 2025. Light-microscopy based connectomic
reconstruction of mammalian brain tissue, Institute of Science and Technology
Austria, 10.15479/AT:ISTA:18697.
mla: Danzl, Johann G., et al. Light-Microscopy Based Connectomic Reconstruction
of Mammalian Brain Tissue. Institute of Science and Technology Austria, 2025,
doi:10.15479/AT:ISTA:18697.
short: J.G. Danzl, J. Lyudchik, C. Kreuzinger, (2025).
contributor:
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first_name: Mojtaba
id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87
last_name: Tavakoli
orcid: 0000-0002-7667-6854
- contributor_type: researcher
first_name: Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- contributor_type: researcher
first_name: Michal
last_name: Januszewski
- contributor_type: researcher
first_name: Vitali
id: 7e146587-8972-11ed-ae7b-d7a32ea86a81
last_name: Vistunou
- contributor_type: researcher
first_name: Nathalie
id: 40E7F008-F248-11E8-B48F-1D18A9856A87
last_name: Agudelo Duenas
- contributor_type: researcher
first_name: Jakob
id: 937696FA-C996-11E9-8C7C-CF13E6697425
last_name: Vorlaufer
- contributor_type: researcher
first_name: Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- contributor_type: researcher
first_name: Caroline
id: 382077BA-F248-11E8-B48F-1D18A9856A87
last_name: Kreuzinger
- contributor_type: researcher
first_name: Bárbara
id: 3B03AA1A-F248-11E8-B48F-1D18A9856A87
last_name: Oliveira
- contributor_type: researcher
first_name: Alban
last_name: Cenameri
- contributor_type: researcher
first_name: Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- contributor_type: researcher
first_name: Viren
last_name: Jain
- contributor_type: researcher
first_name: Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
corr_author: '1'
date_created: 2024-12-20T09:22:20Z
date_published: 2025-03-03T00:00:00Z
date_updated: 2026-01-05T14:11:56Z
day: '03'
ddc:
- '570'
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has_accepted_license: '1'
month: '03'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
link:
- description: Original datasets of large size (>10GB) and datasets in zarr/n5/ims/npz
format are provided via this external link.
relation: research_data
url: https://pub.ista.ac.at/group_danzl/data/LICONN/
record:
- id: '19704'
relation: used_in_publication
status: public
status: public
title: Light-microscopy based connectomic reconstruction of mammalian brain tissue
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
_id: '19857'
abstract:
- lang: eng
text: Bacteria have evolved a wide range of defence strategies to protect themselves
against bacterial viruses (phages). Most known bacterial antiphage defence systems
target phages with DNA genomes, which raises the question of how bacteria defend
against phages with RNA genomes. Bacterial toxin–antitoxin systems that cleave
intracellular RNA could potentially protect bacteria against RNA phages, but this
has not been explored experimentally. In this study, we investigated the role
of a model toxin–antitoxin system, MazEF, in protecting Escherichia coli against
two RNA phage species. When challenged with these phages, the native presence
of mazEF moderately reduced population susceptibility and increased the survival
of individual E. coli cells. Genomic analysis further revealed an underrepresentation
of the MazF cleavage site in genomes of RNA phages infecting E. coli, indicating
selection against cleavage. These results show that, in addition to other physiological
roles, RNA-degrading toxin–antitoxin systems may also help defend against RNA
phages.
acknowledged_ssus:
- _id: LifeSc
acknowledgement: This work was supported by ISTFELLOW (People Program – Marie Curie
Actions of the European Union’s Seventh Framework Program FP7 under REA grant agreement
291734), the FWF (Austrian Science Fund) Elise Richter Program project number V
738 and the Wellcome Trust Institutional Strategic Support Award (WT105618MA), to
N.N. M.P. was a Simons Foundation Fellow of the Life Sciences Research Foundation.
We are grateful to Kathrin Tomasek, Lisa Butt, Chris Estell, Alys Jepson, Franklin
Nobrega, Stefano Pagliara, Remy Chait, Steve West, Vicki Gold, Josh Eaton, Ivana
Gudelj and Rob Beardmore for useful discussions and technical support, as well as
to Robin Wright, Christian Fitch and Ben Temperton for sharing equipment. We thank
Laurence Van Melderen for sharing the strains. We acknowledge the IST Austria Lab
Support Facility, LSI Technical Services Team at the University of Exeter and the
Translational Research Exchange @ Exeter (TREE) network. N.N. is grateful to Fabrice
Gielen for his support.
article_number: '20250080'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Nela
full_name: Nikolic, Nela
id: 42D9CABC-F248-11E8-B48F-1D18A9856A87
last_name: Nikolic
orcid: 0000-0001-9068-6090
- first_name: Maros
full_name: Pleska, Maros
id: 4569785E-F248-11E8-B48F-1D18A9856A87
last_name: Pleska
orcid: 0000-0001-7460-7479
- first_name: Tobias
full_name: Bergmiller, Tobias
id: 2C471CFA-F248-11E8-B48F-1D18A9856A87
last_name: Bergmiller
orcid: 0000-0001-5396-4346
- first_name: Calin C
full_name: Guet, Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
citation:
ama: Nikolic N, Pleska M, Bergmiller T, Guet CC. A bacterial toxin-antitoxin system
as a native defence element against RNA phages. Biology Letters. 2025;21(6).
doi:10.1098/rsbl.2025.0080
apa: Nikolic, N., Pleska, M., Bergmiller, T., & Guet, C. C. (2025). A bacterial
toxin-antitoxin system as a native defence element against RNA phages. Biology
Letters. The Royal Society. https://doi.org/10.1098/rsbl.2025.0080
chicago: Nikolic, Nela, Maros Pleska, Tobias Bergmiller, and Calin C Guet. “A Bacterial
Toxin-Antitoxin System as a Native Defence Element against RNA Phages.” Biology
Letters. The Royal Society, 2025. https://doi.org/10.1098/rsbl.2025.0080.
ieee: N. Nikolic, M. Pleska, T. Bergmiller, and C. C. Guet, “A bacterial toxin-antitoxin
system as a native defence element against RNA phages,” Biology Letters,
vol. 21, no. 6. The Royal Society, 2025.
ista: Nikolic N, Pleska M, Bergmiller T, Guet CC. 2025. A bacterial toxin-antitoxin
system as a native defence element against RNA phages. Biology Letters. 21(6),
20250080.
mla: Nikolic, Nela, et al. “A Bacterial Toxin-Antitoxin System as a Native Defence
Element against RNA Phages.” Biology Letters, vol. 21, no. 6, 20250080,
The Royal Society, 2025, doi:10.1098/rsbl.2025.0080.
short: N. Nikolic, M. Pleska, T. Bergmiller, C.C. Guet, Biology Letters 21 (2025).
corr_author: '1'
date_created: 2025-06-22T22:02:06Z
date_published: 2025-06-11T00:00:00Z
date_updated: 2025-09-30T13:38:08Z
day: '11'
ddc:
- '570'
department:
- _id: CaGu
doi: 10.1098/rsbl.2025.0080
ec_funded: 1
external_id:
isi:
- '001505019800001'
pmid:
- '40494395'
file:
- access_level: open_access
checksum: 016f644ed068f8609ded306ad26dbd3f
content_type: application/pdf
creator: dernst
date_created: 2025-06-23T11:34:39Z
date_updated: 2025-06-23T11:34:39Z
file_id: '19873'
file_name: 2025_BiologyLetters_Nikolic.pdf
file_size: 1850797
relation: main_file
success: 1
file_date_updated: 2025-06-23T11:34:39Z
has_accepted_license: '1'
intvolume: ' 21'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 26956E74-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: V00738
name: Bacterial toxin-antitoxin systems as antiphage defense mechanisms
publication: Biology Letters
publication_identifier:
eissn:
- 1744-957X
issn:
- 1744-9561
publication_status: published
publisher: The Royal Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: A bacterial toxin-antitoxin system as a native defence element against RNA
phages
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 21
year: '2025'
...
---
_id: '19956'
abstract:
- lang: eng
text: The specific introduction of 1H-13C or 1H-15N moieties into otherwise deuterated
proteins holds great potential for high-resolution solution and magic-angle spinning
(MAS) NMR studies of protein structure and dynamics. Arginine residues play key
roles for example at active sites of enzymes. Taking advantage of a chemically
synthesized Arg with a 13C-1H2 group in an otherwise deuterated backbone, we demonstrate
here the usefulness of proton-detected arginine MAS NMR approaches to probe arginine
dynamics. In experiments on crystalline ubiquitin and the 134 kDa tetrameric enzyme
malate dehydrogenase we detected a wide range of motions, from sites that are
rigid on time scales of at least tens of milliseconds to residues undergoing predominantly
nanosecond motions. Spin-relaxation and dipolar-coupling measurements enabled
quantitative determination of these dynamics. We observed microsecond dynamics
of residue Arg54 in crystalline ubiquitin, whose backbone is known to sample different
β-turn conformations on this time scale. The labeling scheme and experiments presented
here expand the toolkit for high-resolution proton-detected MAS NMR
acknowledged_ssus:
- _id: NMR
- _id: LifeSc
article_processing_charge: No
author:
- first_name: Paul
full_name: Schanda, Paul
id: 7B541462-FAF6-11E9-A490-E8DFE5697425
last_name: Schanda
orcid: 0000-0002-9350-7606
citation:
ama: Schanda P. Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific
Isotope-Labeling Scheme. 2025. doi:10.15479/AT-ISTA-19956
apa: Schanda, P. (2025). Arginine Dynamics Probed by Magic-Angle Spinning NMR with
a Specific Isotope-Labeling Scheme. Institute of Science and Technology Austria.
https://doi.org/10.15479/AT-ISTA-19956
chicago: Schanda, Paul. “Arginine Dynamics Probed by Magic-Angle Spinning NMR with
a Specific Isotope-Labeling Scheme.” Institute of Science and Technology Austria,
2025. https://doi.org/10.15479/AT-ISTA-19956.
ieee: P. Schanda, “Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific
Isotope-Labeling Scheme.” Institute of Science and Technology Austria, 2025.
ista: Schanda P. 2025. Arginine Dynamics Probed by Magic-Angle Spinning NMR with
a Specific Isotope-Labeling Scheme, Institute of Science and Technology Austria,
10.15479/AT-ISTA-19956.
mla: Schanda, Paul. Arginine Dynamics Probed by Magic-Angle Spinning NMR with
a Specific Isotope-Labeling Scheme. Institute of Science and Technology Austria,
2025, doi:10.15479/AT-ISTA-19956.
short: P. Schanda, (2025).
contributor:
- contributor_type: researcher
first_name: Darja
last_name: Rohden
- contributor_type: researcher
first_name: Federico
last_name: Napoli
- contributor_type: researcher
first_name: Ben
last_name: Tatman
- contributor_type: researcher
first_name: Paul
last_name: Schanda
corr_author: '1'
date_created: 2025-07-03T04:21:37Z
date_published: 2025-07-03T00:00:00Z
date_updated: 2025-12-29T14:52:16Z
day: '03'
ddc:
- '572'
department:
- _id: PaSc
doi: 10.15479/AT-ISTA-19956
file:
- access_level: open_access
checksum: a2ef61aa9fb5313c7d426913eb0482c0
content_type: application/octet-stream
creator: pschanda
date_created: 2025-07-03T10:30:14Z
date_updated: 2025-07-03T10:30:14Z
file_id: '19960'
file_name: README
file_size: 1160
relation: main_file
success: 1
- access_level: open_access
checksum: 8fb77b96d0fcc95c9903005652207a8c
content_type: application/zip
creator: pschanda
date_created: 2025-07-03T10:30:55Z
date_updated: 2025-07-03T10:30:55Z
file_id: '19961'
file_name: data_Arg_MASNMR_Rohden.zip
file_size: 128597184
relation: main_file
success: 1
- access_level: open_access
checksum: a60cc16d20b089c4bef94040a99cfba5
content_type: application/x-xz
creator: pschanda
date_created: 2025-08-14T07:06:58Z
date_updated: 2025-08-14T07:06:58Z
file_id: '20172'
file_name: 20240903_ubi_DN_Argd1C13_2D_spectra.tar.xz
file_size: 4766564
relation: main_file
success: 1
file_date_updated: 2025-08-14T07:06:58Z
has_accepted_license: '1'
month: '07'
oa: 1
oa_version: None
project:
- _id: eb9c82eb-77a9-11ec-83b8-aadd536561cf
grant_number: I05812
name: AlloSpace. The emergence and mechanisms of allostery
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '20258'
relation: used_in_publication
status: public
status: public
title: Arginine Dynamics Probed by Magic-Angle Spinning NMR with a Specific Isotope-Labeling
Scheme
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: research_data
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '19963'
abstract:
- lang: eng
text: The acquisition of cellular identity requires large-scale alterations in cellular
state. The noncanonical proteasome activator PSME3 is known to regulate diverse
cellular processes, but its importance for differentiation remains unclear. Here,
we demonstrate that PSME3 binds dynamically to highly active promoters over the
course of differentiation. However, loss of PSME3 does not globally affect mRNA
transcription. We find instead that PSME3 influences the levels of several adhesion-related
proteins and acts upstream of the HSP90 co-chaperone NUDC to regulate cell motility
and myoblast differentiation in a proteasome-independent manner. Our findings
reveal several new facets of PSME3 functionality and highlight its importance
for the differentiation of myogenic cells.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: 'All proteomics analysis was done by the ISTA LSF Mass Spectrometry
Service: Ewelina Dutkiewicz-Kopczynska processed the samples (digest and cleanup);
Bella Bruszel optimized the acquisition methods, acquired the data, and performed
all searches; and Armel Nicolas provided pre- and post-project consulting and post-processed
the search results using a development version of their data analysis package, proteoCraft
(publication pending). The authors would like to thank Saki for their clarity of
thought and insight, as well as Dr. Lorenzo Puri and the members of his laboratory
for invaluable discussions relating to the project. This research was further supported
by the Lab Support Facility and the Imaging and Optics Facility of ISTA.'
article_number: e202503208
article_processing_charge: Yes
article_type: original
author:
- first_name: Kenneth D
full_name: Kuhn, Kenneth D
id: 7deed7e0-0133-11f0-8590-c4600b08d0f4
last_name: Kuhn
- first_name: Ukrae H.
full_name: Cho, Ukrae H.
last_name: Cho
- first_name: Martin W
full_name: Hetzer, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: Hetzer
orcid: 0000-0002-2111-992X
citation:
ama: Kuhn KD, Cho UH, Hetzer M. PSME3 regulates migration and differentiation of
myoblasts. Life Science Alliance. 2025;8(9). doi:10.26508/lsa.202503208
apa: Kuhn, K. D., Cho, U. H., & Hetzer, M. (2025). PSME3 regulates migration
and differentiation of myoblasts. Life Science Alliance. Embo Press. https://doi.org/10.26508/lsa.202503208
chicago: Kuhn, Kenneth D, Ukrae H. Cho, and Martin Hetzer. “PSME3 Regulates Migration
and Differentiation of Myoblasts.” Life Science Alliance. Embo Press, 2025.
https://doi.org/10.26508/lsa.202503208.
ieee: K. D. Kuhn, U. H. Cho, and M. Hetzer, “PSME3 regulates migration and differentiation
of myoblasts,” Life Science Alliance, vol. 8, no. 9. Embo Press, 2025.
ista: Kuhn KD, Cho UH, Hetzer M. 2025. PSME3 regulates migration and differentiation
of myoblasts. Life Science Alliance. 8(9), e202503208.
mla: Kuhn, Kenneth D., et al. “PSME3 Regulates Migration and Differentiation of
Myoblasts.” Life Science Alliance, vol. 8, no. 9, e202503208, Embo Press,
2025, doi:10.26508/lsa.202503208.
short: K.D. Kuhn, U.H. Cho, M. Hetzer, Life Science Alliance 8 (2025).
corr_author: '1'
date_created: 2025-07-06T22:01:22Z
date_published: 2025-09-01T00:00:00Z
date_updated: 2025-12-30T09:17:55Z
day: '01'
ddc:
- '570'
department:
- _id: MaHe
doi: 10.26508/lsa.202503208
external_id:
isi:
- '001511452100001'
pmid:
- '40537284'
file:
- access_level: open_access
checksum: 591d47aa39fc969986c7d3b966890f5f
content_type: application/pdf
creator: dernst
date_created: 2025-12-30T09:17:09Z
date_updated: 2025-12-30T09:17:09Z
file_id: '20904'
file_name: 2025_LifeScienceAlliance_Kuhn.pdf
file_size: 5471288
relation: main_file
success: 1
file_date_updated: 2025-12-30T09:17:09Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '9'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Life Science Alliance
publication_identifier:
eissn:
- 2575-1077
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: PSME3 regulates migration and differentiation of myoblasts
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2025'
...
---
OA_type: closed access
_id: '20055'
abstract:
- lang: eng
text: Supercrystals represent three-dimensional orderings of colloidal nanocrystals
(NCs), showcasing collective properties in photonics, phononics, and electronics
applications.1,2 Recent studies have shown that such assemblies are directly produced
during nanocrystal reactions.3–6 However, a fundamental understanding of in situ
formed supercrystals that withstand typical NC purification processes remains
underexplored, which is important for further use. Herein, we report the reaction
precursor-mediated formation of stable PbTe supercrystals. Rationalizing the formation
of these assemblies through small-angle x-ray scattering (SAXS) measurements,
we unveil their formation mechanism. Our findings reveal that the supercrystal
formation occurs in the presence of an excess of lead oleates in the crude solution.
It should be noted that the formed supercrystals can be stabilized under specific
conditions determined by the lead oleate cluster concentration, content of trioctylphosphine
telluride (TOP-Te), NC size and the need of an annealing step at mild conditions.
Furthermore, this approach allows for the continuous growth of a secondary phase
within the supercrystal; for example in the case of PbTe supercrystals, a PbS
shell can be grown on each PbTe NC constituent, resulting in core-shell PbTe-PbS
supercrystals. Our work elucidates that reaction precursors play an important
role in in situ SC formation and stabilization, implying the possibility of applying
this knowledge to other NC reactions.
acknowledged_ssus:
- _id: EM-Fac
- _id: NMR
- _id: LifeSc
acknowledgement: ISTA and the Werner Siemens Foundation financially supported this
work. The Scientific Service Units (SSU) of ISTA supported this research through
resources provided by the Electron Microscopy Facility (EMF), NMR Facility and the
Lab Support Facility (LSF).
article_number: '173'
article_processing_charge: No
author:
- first_name: Seungho
full_name: Lee, Seungho
id: BB243B88-D767-11E9-B658-BC13E6697425
last_name: Lee
orcid: 0000-0002-6962-8598
- first_name: Daniel
full_name: Balazs, Daniel
id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E
last_name: Balazs
orcid: 0000-0001-7597-043X
- first_name: Sharona
full_name: Horta, Sharona
id: 03a7e858-01b1-11ec-8b71-99ae6c4a05bc
last_name: Horta
- first_name: Aiswarya
full_name: Rayaroth Puthiyaveettil, Aiswarya
id: 8aceb01b-8972-11ed-ae7b-d5fe53775add
last_name: Rayaroth Puthiyaveettil
- first_name: Maria
full_name: Ibáñez, Maria
id: 43C61214-F248-11E8-B48F-1D18A9856A87
last_name: Ibáñez
orcid: 0000-0001-5013-2843
citation:
ama: 'Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. Reaction precursor-mediated
formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case.
In: Proceedings of the MATSUS Spring 2025 Conference. Fundació de la comunitat
valenciana SCITO; 2025. doi:10.29363/nanoge.matsusspring.2025.173'
apa: 'Lee, S., Balazs, D., Horta, S., Rayaroth Puthiyaveettil, A., & Ibáñez,
M. (2025). Reaction precursor-mediated formation of stable supercrystals in colloidal
nanocrystal synthesis: PbTe case. In Proceedings of the MATSUS Spring 2025
Conference. Sevilla, Spain: Fundació de la comunitat valenciana SCITO. https://doi.org/10.29363/nanoge.matsusspring.2025.173'
chicago: 'Lee, Seungho, Daniel Balazs, Sharona Horta, Aiswarya Rayaroth Puthiyaveettil,
and Maria Ibáñez. “Reaction Precursor-Mediated Formation of Stable Supercrystals
in Colloidal Nanocrystal Synthesis: PbTe Case.” In Proceedings of the MATSUS
Spring 2025 Conference. Fundació de la comunitat valenciana SCITO, 2025. https://doi.org/10.29363/nanoge.matsusspring.2025.173.'
ieee: 'S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, and M. Ibáñez, “Reaction
precursor-mediated formation of stable supercrystals in colloidal nanocrystal
synthesis: PbTe case,” in Proceedings of the MATSUS Spring 2025 Conference,
Sevilla, Spain, 2025.'
ista: 'Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. 2025. Reaction
precursor-mediated formation of stable supercrystals in colloidal nanocrystal
synthesis: PbTe case. Proceedings of the MATSUS Spring 2025 Conference. MATSUS:
Materials for Sustainable Development Conference, 173.'
mla: 'Lee, Seungho, et al. “Reaction Precursor-Mediated Formation of Stable Supercrystals
in Colloidal Nanocrystal Synthesis: PbTe Case.” Proceedings of the MATSUS Spring
2025 Conference, 173, Fundació de la comunitat valenciana SCITO, 2025, doi:10.29363/nanoge.matsusspring.2025.173.'
short: S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, M. Ibáñez, in:,
Proceedings of the MATSUS Spring 2025 Conference, Fundació de la comunitat valenciana
SCITO, 2025.
conference:
end_date: 2025-03-07
location: Sevilla, Spain
name: 'MATSUS: Materials for Sustainable Development Conference'
start_date: 2025-03-03
corr_author: '1'
date_created: 2025-07-21T08:33:20Z
date_published: 2025-03-15T00:00:00Z
date_updated: 2026-02-19T09:25:57Z
day: '15'
department:
- _id: MaIb
- _id: LifeSc
doi: 10.29363/nanoge.matsusspring.2025.173
language:
- iso: eng
month: '03'
oa_version: None
project:
- _id: 9B8F7476-BA93-11EA-9121-9846C619BF3A
name: 'HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of
Semiconductors for Waste Heat Recovery'
publication: Proceedings of the MATSUS Spring 2025 Conference
publication_status: published
publisher: Fundació de la comunitat valenciana SCITO
quality_controlled: '1'
status: public
title: 'Reaction precursor-mediated formation of stable supercrystals in colloidal
nanocrystal synthesis: PbTe case'
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20080'
abstract:
- lang: eng
text: "Introduction: Acid-growth theory has been postulated in the 70s to explain
the rapid elongation of plant cells in response to the hormone auxin. More recently,
it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting
acidification of the apoplast is the principal mechanism by which auxin and other
hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances,
the impact of this acidification on the mechanical properties of the cell wall
remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of
Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate
hormone-induced tissue elongation and local changes in cell wall mechanical properties.
Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase
(PME), along with calcium chelators, we investigate the effect of pectin modification
in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification
of apoplast is necessary and sufficient to induce cell elongation through promoting
cell wall softening. Moreover, we show that enhanced PME activity can induce both
cell wall softening or stiffening in extracellular calcium dependent-manner and
that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion:
Our results confirm a dual role of PME in plant cell elongation. However, further
investigation is needed to assess the status of pectin following short- or long-term
PME treatments in order to determine if pectin methyl-esterification might promote
its degradation as well as the role of PME inhibitors upon PME induction."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: E-Lib
acknowledgement: "The author(s) declare that financial support was received for the
research and/or publication of this article. This work was supported by grants from
the European Research Council (Starting Independent Research Grant ERC-2007-Stg-
207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe
acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge
CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127).
We gratefully acknowledge support by the Scientific Service Units at ISTA, including
the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler
for the efforts to establish immunodetection method."
article_number: '1612366'
article_processing_charge: Yes
article_type: original
author:
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Nikola
full_name: Zarevski, Nikola
id: 18e95355-e05a-11ea-a9c0-8fba1b89e83a
last_name: Zarevski
- first_name: Jan
full_name: Pribyl, Jan
last_name: Pribyl
- first_name: Petr
full_name: Skládal, Petr
last_name: Skládal
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl
esterase activity in the regulation of plant cell wall biophysical properties.
Frontiers in Plant Science. 2025;16. doi:10.3389/fpls.2025.1612366
apa: Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P.,
Hannezo, E. B., & Benková, E. (2025). Dual role of pectin methyl esterase
activity in the regulation of plant cell wall biophysical properties. Frontiers
in Plant Science. Frontiers Media. https://doi.org/10.3389/fpls.2025.1612366
chicago: Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl,
Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl
Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.”
Frontiers in Plant Science. Frontiers Media, 2025. https://doi.org/10.3389/fpls.2025.1612366.
ieee: M. Gallemi et al., “Dual role of pectin methyl esterase activity in
the regulation of plant cell wall biophysical properties,” Frontiers in Plant
Science, vol. 16. Frontiers Media, 2025.
ista: Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB,
Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation
of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.
mla: Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the
Regulation of Plant Cell Wall Biophysical Properties.” Frontiers in Plant Science,
vol. 16, 1612366, Frontiers Media, 2025, doi:10.3389/fpls.2025.1612366.
short: M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B.
Hannezo, E. Benková, Frontiers in Plant Science 16 (2025).
corr_author: '1'
date_created: 2025-07-27T22:01:26Z
date_published: 2025-07-04T00:00:00Z
date_updated: 2025-09-30T14:08:22Z
day: '04'
ddc:
- '580'
department:
- _id: EdHa
- _id: EvBe
- _id: CaGu
doi: 10.3389/fpls.2025.1612366
ec_funded: 1
external_id:
isi:
- '001530690900001'
pmid:
- '40688689'
file:
- access_level: open_access
checksum: 9e6b8b53ba56d4a24a9bd91cf6d2dc58
content_type: application/pdf
creator: dernst
date_created: 2025-07-31T07:28:54Z
date_updated: 2025-07-31T07:28:54Z
file_id: '20093'
file_name: 2025_FrontiersPlantSc_Gallemi.pdf
file_size: 3665187
relation: main_file
success: 1
file_date_updated: 2025-07-31T07:28:54Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664-462X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dual role of pectin methyl esterase activity in the regulation of plant cell
wall biophysical properties
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 16
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20099'
abstract:
- lang: eng
text: The hippocampus, critical for learning and memory, is dogmatically described
as a trisynaptic circuit where dentate gyrus granule cells (GCs), CA3 pyramidal
neurons (PNs), and CA1 PNs are serially connected. However, CA3 also forms an
autoassociative network, and its PNs have diverse morphologies, intrinsic properties,
and GC input levels. How PN subtypes compose this recurrent network is unknown.
To determine the synaptic arrangement of identified CA3 PNs, we combine multicellular
patch-clamp recording and post hoc morphological analysis in mouse hippocampal
slices. PNs can be divided into distinct “superficial” and “deep” subclasses,
the latter including previously reported “athorny” cells. Subclasses have distinct
input-output transformations and asymmetric connectivity, which is more abundant
from superficial to deep PNs, splitting CA3 locally into two parallel recurrent
networks. Coincident spontaneous inhibition occurs frequently within but not between
subclasses, implying subclass-specific inhibitory innervation. Our results suggest
two separately controlled sublayers for parallel information processing in hippocampal
CA3.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
- _id: LifeSc
- _id: M-Shop
acknowledgement: We thank Andrea Navas-Olive and Rebecca J. Morse-Mora for critically
reading an earlier version of the manuscript. We also thank Florian Marr and Christina
Altmutter for excellent technical assistance, Alois Schlögl for programming and
data-handling assistance, Todor Asenov for technical support, and Eleftheria Kralli-Beller
for manuscript editing. This research was supported by the Scientific Services Units
(SSUs) of ISTA. We are particularly grateful for assistance from the Imaging and
Optics Facility, Preclinical Facility, Lab Support Facility, and Miba Machine Shop.
The project received funding from the European Research Council (ERC) under the
European Union’s Horizon 2020 research and innovation program (grant agreement no.
692692 to P.J., Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635
to J.F.W., and an ISTplus Fellowship through Marie Skłodowska-Curie grant agreement
no. 754411 to V.V.-B.), the Austrian Science Fund (P 36232-B, PAT 4178023, and Cluster
of Excellence 10.55776/COE16 to P.J.), and a CONACyT fellowship (289638 to V.V.-B.)
and was supported by a non-stipendiary EMBO fellowship (ALTF 756–2020 to J.F.W.).
article_number: '116080'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Victor M
full_name: Vargas Barroso, Victor M
id: 2F55A9DE-F248-11E8-B48F-1D18A9856A87
last_name: Vargas Barroso
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Watson J, Vargas Barroso VM, Jonas PM. Cell-specific wiring routes information
flow through hippocampal CA3. Cell Reports. 2025;44(8). doi:10.1016/j.celrep.2025.116080
apa: Watson, J., Vargas Barroso, V. M., & Jonas, P. M. (2025). Cell-specific
wiring routes information flow through hippocampal CA3. Cell Reports. Elsevier.
https://doi.org/10.1016/j.celrep.2025.116080
chicago: Watson, Jake, Victor M Vargas Barroso, and Peter M Jonas. “Cell-Specific
Wiring Routes Information Flow through Hippocampal CA3.” Cell Reports.
Elsevier, 2025. https://doi.org/10.1016/j.celrep.2025.116080.
ieee: J. Watson, V. M. Vargas Barroso, and P. M. Jonas, “Cell-specific wiring routes
information flow through hippocampal CA3,” Cell Reports, vol. 44, no. 8.
Elsevier, 2025.
ista: Watson J, Vargas Barroso VM, Jonas PM. 2025. Cell-specific wiring routes information
flow through hippocampal CA3. Cell Reports. 44(8), 116080.
mla: Watson, Jake, et al. “Cell-Specific Wiring Routes Information Flow through
Hippocampal CA3.” Cell Reports, vol. 44, no. 8, 116080, Elsevier, 2025,
doi:10.1016/j.celrep.2025.116080.
short: J. Watson, V.M. Vargas Barroso, P.M. Jonas, Cell Reports 44 (2025).
corr_author: '1'
date_created: 2025-08-03T22:01:30Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2025-09-30T14:12:02Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.celrep.2025.116080
ec_funded: 1
external_id:
isi:
- '001544472300002'
file:
- access_level: open_access
checksum: 556ff9760661ecd23949d75031043b1f
content_type: application/pdf
creator: dernst
date_created: 2025-08-04T06:53:07Z
date_updated: 2025-08-04T06:53:07Z
file_id: '20106'
file_name: 2025_CellReports_Watson.pdf
file_size: 27695214
relation: main_file
success: 1
file_date_updated: 2025-08-04T06:53:07Z
has_accepted_license: '1'
intvolume: ' 44'
isi: 1
issue: '8'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: fc2be41b-9c52-11eb-aca3-faa90aa144e9
call_identifier: H2020
grant_number: '101026635'
name: Synaptic computations of the hippocampal CA3 circuitry
- _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5
grant_number: P36232
name: Mechanisms of GABA release in hippocampal circuits
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Cell Reports
publication_identifier:
eissn:
- 2211-1247
issn:
- 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell-specific wiring routes information flow through hippocampal CA3
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 44
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20258'
abstract:
- lang: eng
text: The specific introduction of ^1H-^13C or ^1H-^15N moieties into otherwise
deuterated proteins holds great potential for high-resolution solution and magic-angle
spinning (MAS) NMR studies of protein structure and dynamics. Arginine residues
play key roles for example at active sites of enzymes. Taking advantage of a chemically
synthesized Arg with a ^13C-^1H2 group in an otherwise deuterated backbone, we
demonstrate here the usefulness of proton-detected MAS NMR approaches to probe
arginine dynamics. In experiments with crystalline ubiquitin and the 134 kDa tetrameric
enzyme malate dehydrogenase we detected a wide range of motions, from sites that
are rigid on time scales of at least tens of milliseconds to residues undergoing
predominantly nanosecond motions. Spin-relaxation and dipolar-coupling measurements
enabled quantitative determination of these dynamics. We observed microsecond
dynamics of residue Arg54 in crystalline ubiquitin, whose backbone is known to
sample different β-turn conformations on this time scale. The labeling scheme
and experiments presented here expand the toolkit for high-resolution proton-detected
MAS NMR.
acknowledged_ssus:
- _id: NMR
- _id: LifeSc
acknowledgement: This work was supported financially by the Austrian Science Fund
(FWF, Grant No. I5812-B, “AlloSpace”). This research was supported by the Scientific
Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through
resources provided by the Nuclear Magnetic Resonance Facility and the Lab Support
Facility (LSF). We thank Petra Rovò and Margarita Valhondo Falcón for excellent
support of the NMR facility.
article_number: '169379'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Darja
full_name: Rohden, Darja
id: 81dc668a-19fa-11f0-bf31-d56534059ef3
last_name: Rohden
- first_name: Federico
full_name: Napoli, Federico
id: d42e08e7-f4fc-11eb-af0a-d71e26138f1b
last_name: Napoli
orcid: 0000-0002-9043-136X
- first_name: Anna
full_name: Kapitonova, Anna
id: 9fb2a840-89e1-11ee-a8b7-cc5c7ba62471
last_name: Kapitonova
- first_name: Benjamin
full_name: Tatman, Benjamin
id: 71cda2f3-e604-11ee-a1df-da10587eda3f
last_name: Tatman
- first_name: Roman J.
full_name: Lichtenecker, Roman J.
last_name: Lichtenecker
- first_name: Paul
full_name: Schanda, Paul
id: 7B541462-FAF6-11E9-A490-E8DFE5697425
last_name: Schanda
orcid: 0000-0002-9350-7606
citation:
ama: Rohden D, Napoli F, Kapitonova A, Tatman B, Lichtenecker RJ, Schanda P. Arginine
dynamics probed by magic-angle spinning NMR with a specific isotope-labeling scheme.
Journal of Molecular Biology. 2025;437(23). doi:10.1016/j.jmb.2025.169379
apa: Rohden, D., Napoli, F., Kapitonova, A., Tatman, B., Lichtenecker, R. J., &
Schanda, P. (2025). Arginine dynamics probed by magic-angle spinning NMR with
a specific isotope-labeling scheme. Journal of Molecular Biology. Elsevier.
https://doi.org/10.1016/j.jmb.2025.169379
chicago: Rohden, Darja, Federico Napoli, Anna Kapitonova, Benjamin Tatman, Roman
J. Lichtenecker, and Paul Schanda. “Arginine Dynamics Probed by Magic-Angle Spinning
NMR with a Specific Isotope-Labeling Scheme.” Journal of Molecular Biology.
Elsevier, 2025. https://doi.org/10.1016/j.jmb.2025.169379.
ieee: D. Rohden, F. Napoli, A. Kapitonova, B. Tatman, R. J. Lichtenecker, and P.
Schanda, “Arginine dynamics probed by magic-angle spinning NMR with a specific
isotope-labeling scheme,” Journal of Molecular Biology, vol. 437, no. 23.
Elsevier, 2025.
ista: Rohden D, Napoli F, Kapitonova A, Tatman B, Lichtenecker RJ, Schanda P. 2025.
Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling
scheme. Journal of Molecular Biology. 437(23), 169379.
mla: Rohden, Darja, et al. “Arginine Dynamics Probed by Magic-Angle Spinning NMR
with a Specific Isotope-Labeling Scheme.” Journal of Molecular Biology,
vol. 437, no. 23, 169379, Elsevier, 2025, doi:10.1016/j.jmb.2025.169379.
short: D. Rohden, F. Napoli, A. Kapitonova, B. Tatman, R.J. Lichtenecker, P. Schanda,
Journal of Molecular Biology 437 (2025).
corr_author: '1'
date_created: 2025-08-31T22:01:33Z
date_published: 2025-12-01T00:00:00Z
date_updated: 2025-12-29T14:52:17Z
day: '01'
ddc:
- '540'
department:
- _id: PaSc
doi: 10.1016/j.jmb.2025.169379
external_id:
isi:
- '001618289100020'
file:
- access_level: open_access
checksum: 90d50594d8ea9860ac5da41297992847
content_type: application/pdf
creator: dernst
date_created: 2025-12-29T14:51:40Z
date_updated: 2025-12-29T14:51:40Z
file_id: '20876'
file_name: 2025_JourMolecularBiology_Rohden.pdf
file_size: 2270555
relation: main_file
success: 1
file_date_updated: 2025-12-29T14:51:40Z
has_accepted_license: '1'
intvolume: ' 437'
isi: 1
issue: '23'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
project:
- _id: eb9c82eb-77a9-11ec-83b8-aadd536561cf
grant_number: I05812
name: AlloSpace. The emergence and mechanisms of allostery
publication: Journal of Molecular Biology
publication_identifier:
eissn:
- 1089-8638
issn:
- 0022-2836
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '19956'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Arginine dynamics probed by magic-angle spinning NMR with a specific isotope-labeling
scheme
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 437
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20289'
abstract:
- lang: eng
text: Cell and tissue movement in development, cancer invasion, and immune response
relies on chemical or mechanical guidance cues. In many systems, this behavior
is locally directed by self-generated signaling gradients rather than long-range,
prepatterned cues. However, how heterogeneous mixtures of cells interact nonreciprocally
and navigate through self-generated gradients remains largely unexplored. Here,
we introduce a theoretical framework for the self-organized chemotaxis of heterogeneous
cell populations. We find that the relative chemotactic sensitivities of different
cell populations control their long-time coupling and comigration dynamics, with
boundary conditions such as external cell and attractant reservoirs substantially
influencing the migration patterns. Our model predicts an optimal parameter regime
that enables robust and colocalized migration. We test our theoretical predictions
with in vitro experiments demonstrating the comigration of distinct immune cell
populations, and quantitatively reproduce observed migration patterns under wild-type
and perturbed conditions. Interestingly, immune cell comigration occurs close
to the predicted optimal regime. Finally, we incorporate mechanical interactions
into our framework, revealing a nontrivial interplay between chemotactic and mechanical
nonreciprocity in driving collective migration. Together, our findings suggest
that self-generated chemotaxis is a robust strategy for the navigation of mixed
cell populations.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
- _id: LifeSc
- _id: NanoFab
acknowledgement: We thank all members of the M.S. and E.H. groups for stimulating
discussions.We thank the Imaging and Optics facility, the Pre-clinical and Lab Support
facility of the Institute of Science and Technology Austria for their excellent
support and provided resources for the experimental research. In particular, we
thank Jack Merrin from the Nanofabrication facility who generated the microfabricated
channel used in this study. This work received funding fromt he European Research
Council under the European Union’s Horizon 2020 research and innovation program
(grant agreement No. 851288 to E.H.). M.C.U.is funded by a University of Sheffield
Strategic Research Fellowship in the Physics of Life and Quantitative Biology.
article_number: e2504064122
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Mehmet C
full_name: Ucar, Mehmet C
id: 50B2A802-6007-11E9-A42B-EB23E6697425
last_name: Ucar
orcid: 0000-0003-0506-4217
- first_name: Alsberga
full_name: Zane, Alsberga
id: 60f7509a-f652-11ea-9d86-b963d6490d7c
last_name: Zane
orcid: 0009-0003-0415-7603
- first_name: Jonna H
full_name: Alanko, Jonna H
id: 2CC12E8C-F248-11E8-B48F-1D18A9856A87
last_name: Alanko
orcid: 0000-0002-7698-3061
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. Self-generated chemotaxis
of mixed cell populations. Proceedings of the National Academy of Sciences.
2025;122(34). doi:10.1073/pnas.2504064122
apa: Ucar, M. C., Zane, A., Alanko, J. H., Sixt, M. K., & Hannezo, E. B. (2025).
Self-generated chemotaxis of mixed cell populations. Proceedings of the National
Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2504064122
chicago: Ucar, Mehmet C, Alsberga Zane, Jonna H Alanko, Michael K Sixt, and Edouard
B Hannezo. “Self-Generated Chemotaxis of Mixed Cell Populations.” Proceedings
of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2504064122.
ieee: M. C. Ucar, A. Zane, J. H. Alanko, M. K. Sixt, and E. B. Hannezo, “Self-generated
chemotaxis of mixed cell populations,” Proceedings of the National Academy
of Sciences, vol. 122, no. 34. National Academy of Sciences, 2025.
ista: Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. 2025. Self-generated chemotaxis
of mixed cell populations. Proceedings of the National Academy of Sciences. 122(34),
e2504064122.
mla: Ucar, Mehmet C., et al. “Self-Generated Chemotaxis of Mixed Cell Populations.”
Proceedings of the National Academy of Sciences, vol. 122, no. 34, e2504064122,
National Academy of Sciences, 2025, doi:10.1073/pnas.2504064122.
short: M.C. Ucar, A. Zane, J.H. Alanko, M.K. Sixt, E.B. Hannezo, Proceedings of
the National Academy of Sciences 122 (2025).
corr_author: '1'
date_created: 2025-09-07T22:01:32Z
date_published: 2025-08-26T00:00:00Z
date_updated: 2026-02-16T12:31:05Z
day: '26'
ddc:
- '570'
department:
- _id: EdHa
- _id: MiSi
doi: 10.1073/pnas.2504064122
ec_funded: 1
external_id:
isi:
- '001562181600001'
pmid:
- '40838890'
file:
- access_level: open_access
checksum: b36abd92673b6d76376fc9434bad52cc
content_type: application/pdf
creator: dernst
date_created: 2025-09-08T07:23:29Z
date_updated: 2025-09-08T07:23:29Z
file_id: '20307'
file_name: 2025_PNAS_Ucar.pdf
file_size: 16069140
relation: main_file
success: 1
file_date_updated: 2025-09-08T07:23:29Z
has_accepted_license: '1'
intvolume: ' 122'
isi: 1
issue: '34'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/mehmetcanucar/Self-generated-chemotaxis
scopus_import: '1'
status: public
title: Self-generated chemotaxis of mixed cell populations
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 122
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20295'
abstract:
- lang: eng
text: 'Scanning Kelvin probe microscopy (SKPM) is a powerful technique for macroscopic
imaging of the electrostatic potential above a surface. Though most often used
to image work-function variations of conductive surfaces, it can also be used
to probe the surface charge on insulating surfaces. In both cases, relating the
measured potential to the underlying signal is non-trivial. Here, general relationships
are derived between the measured SKPM voltage and the underlying source, revealing
either can be cast as a convolution with an appropriately scaled point spread
function (PSF). For charge that exists on a thin insulating layer above a conductor,
the PSF has the same shape as what would occur from a work-function variation
alone, differing by a simple scaling factor. This relationship is confirmed by:
(1) backing it out from finite-element simulations of work-function and charge
signals, and (2) experimentally comparing the measured PSF from a small work-function
target to that from a small charge spot. This scaling factor is further validated
by comparing SKPM charge measurements with Faraday cup measurements for highly
charged samples from contact-charging experiments. These results highlight a heretofore
unappreciated connection between SKPM voltage and charge signals, offering a rigorous
recipe to extract either from experimental data.'
acknowledged_ssus:
- _id: M-Shop
- _id: NanoFab
- _id: ScienComp
- _id: LifeSc
acknowledgement: This project received funding from the European Research Council
(ERC) under the European Union's Horizon 2020 research and innovation programme
(Grant agreement No. 949120). This research was supported by the Scientific Service
Units of The Institute of Science and Technology Austria (ISTA) through resources
provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing
Facility, and Lab Support Facility. The authors wish to thank Dmytro Rak and Juan
Carlos Sobarzo for letting us use their equipment. The authors wish to thank Evgeniia
Volobueva for advice in preparing PFIB samples. The authors wish to thank the contributions
of the whole Waitukaitis group for useful discussions and feedback.
article_number: e00521
article_processing_charge: Yes
article_type: original
arxiv: 1
author:
- first_name: Isaac C
full_name: Lenton, Isaac C
id: a550210f-223c-11ec-8182-e2d45e817efb
last_name: Lenton
orcid: 0000-0002-5010-6984
- first_name: Felix
full_name: Pertl, Felix
id: 6313aec0-15b2-11ec-abd3-ed67d16139af
last_name: Pertl
orcid: 0000-0003-0463-5794
- first_name: Lubuna B
full_name: Shafeek, Lubuna B
id: 3CD37A82-F248-11E8-B48F-1D18A9856A87
last_name: Shafeek
orcid: 0000-0001-7180-6050
- first_name: Scott R
full_name: Waitukaitis, Scott R
id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87
last_name: Waitukaitis
orcid: 0000-0002-2299-3176
citation:
ama: Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. A duality between surface charge
and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces.
2025;12(19). doi:10.1002/admi.202500521
apa: Lenton, I. C., Pertl, F., Shafeek, L. B., & Waitukaitis, S. R. (2025).
A duality between surface charge and work function in scanning Kelvin probe microscopy.
Advanced Materials Interfaces. Wiley. https://doi.org/10.1002/admi.202500521
chicago: Lenton, Isaac C, Felix Pertl, Lubuna B Shafeek, and Scott R Waitukaitis.
“A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.”
Advanced Materials Interfaces. Wiley, 2025. https://doi.org/10.1002/admi.202500521.
ieee: I. C. Lenton, F. Pertl, L. B. Shafeek, and S. R. Waitukaitis, “A duality between
surface charge and work function in scanning Kelvin probe microscopy,” Advanced
Materials Interfaces, vol. 12, no. 19. Wiley, 2025.
ista: Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. 2025. A duality between surface
charge and work function in scanning Kelvin probe microscopy. Advanced Materials
Interfaces. 12(19), e00521.
mla: Lenton, Isaac C., et al. “A Duality between Surface Charge and Work Function
in Scanning Kelvin Probe Microscopy.” Advanced Materials Interfaces, vol.
12, no. 19, e00521, Wiley, 2025, doi:10.1002/admi.202500521.
short: I.C. Lenton, F. Pertl, L.B. Shafeek, S.R. Waitukaitis, Advanced Materials
Interfaces 12 (2025).
corr_author: '1'
date_created: 2025-09-07T22:01:33Z
date_published: 2025-10-01T00:00:00Z
date_updated: 2025-12-30T09:31:25Z
day: '01'
ddc:
- '530'
department:
- _id: ScWa
- _id: NanoFab
doi: 10.1002/admi.202500521
ec_funded: 1
external_id:
arxiv:
- '2506.07187'
isi:
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title: A duality between surface charge and work function in scanning Kelvin probe
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