[{"DOAJ_listed":"1","intvolume":"        17","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","citation":{"ama":"Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. Flexoelectric domain walls enable charge separation and transport in cubic perovskites. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-68660-5\">10.1038/s41467-026-68660-5</a>","apa":"Rak, D., Lorenc, D., Balazs, D., Zhumekenov, A. A., Bakr, O. M., &#38; Alpichshev, Z. (2026). Flexoelectric domain walls enable charge separation and transport in cubic perovskites. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-68660-5\">https://doi.org/10.1038/s41467-026-68660-5</a>","ieee":"D. Rak, D. Lorenc, D. Balazs, A. A. Zhumekenov, O. M. Bakr, and Z. Alpichshev, “Flexoelectric domain walls enable charge separation and transport in cubic perovskites,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026.","ista":"Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. 2026. Flexoelectric domain walls enable charge separation and transport in cubic perovskites. Nature Communications. 17, 946.","mla":"Rak, Dmytro, et al. “Flexoelectric Domain Walls Enable Charge Separation and Transport in Cubic Perovskites.” <i>Nature Communications</i>, vol. 17, 946, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-68660-5\">10.1038/s41467-026-68660-5</a>.","short":"D. Rak, D. Lorenc, D. Balazs, A.A. Zhumekenov, O.M. Bakr, Z. Alpichshev, Nature Communications 17 (2026).","chicago":"Rak, Dmytro, Dusan Lorenc, Daniel Balazs, Ayan A. Zhumekenov, Osman M. Bakr, and Zhanybek Alpichshev. “Flexoelectric Domain Walls Enable Charge Separation and Transport in Cubic Perovskites.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-68660-5\">https://doi.org/10.1038/s41467-026-68660-5</a>."},"date_published":"2026-02-16T00:00:00Z","article_number":"946","corr_author":"1","status":"public","department":[{"_id":"ZhAl"},{"_id":"LifeSc"}],"file_date_updated":"2026-03-02T14:27:56Z","license":"https://creativecommons.org/licenses/by/4.0/","publisher":"Springer Nature","scopus_import":"1","volume":17,"date_created":"2026-03-02T10:06:58Z","date_updated":"2026-04-28T12:12:46Z","quality_controlled":"1","_id":"21382","month":"02","oa_version":"Published Version","doi":"10.1038/s41467-026-68660-5","type":"journal_article","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","PlanS_conform":"1","related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/explaining-next-generation-solar-cells/"}]},"language":[{"iso":"eng"}],"external_id":{"pmid":["41698893"]},"pmid":1,"article_type":"original","year":"2026","publication":"Nature Communications","acknowledged_ssus":[{"_id":"Bio"},{"_id":"M-Shop"}],"file":[{"checksum":"dd7a98de892d0b5abefca7e290ca0f77","file_name":"2026_NatureComm_Rak.pdf","file_id":"21390","relation":"main_file","content_type":"application/pdf","access_level":"open_access","success":1,"creator":"dernst","file_size":2570918,"date_updated":"2026-03-02T14:27:56Z","date_created":"2026-03-02T14:27:56Z"}],"publication_identifier":{"eissn":["2041-1723"]},"title":"Flexoelectric domain walls enable charge separation and transport in cubic perovskites","abstract":[{"lang":"eng","text":"The exceptional energy-harvesting efficiency of lead-halide perovskites arises from unusually long photocarrier diffusion lengths and recombination lifetimes that persist even in defect-rich, solution-grown samples. Paradoxically, perovskites are also known for having very short exciton decay times. Here, we resolve this apparent contradiction by showing that key optoelectronic properties of perovskites can be explained by localized flexoelectric polarization confined to interfaces between domains of spontaneous strain. Using birefringence imaging, electrochemical staining, and zero-bias photocurrent measurements, we visualize the domain structure and directly probe the associated internal fields in nominally cubic single crystals of methylammonium lead bromide. We demonstrate that localized flexoelectric fields spatially separate electrons and holes to opposite sides of domain walls, exponentially suppressing recombination. Domain walls thus act as efficient mesoscopic transport channels for long-lived photocarriers, microscopically linking structural heterogeneity to charge transport and offering mechanistically informed design principles for perovskite solar-energy technologies."}],"acknowledgement":"We are grateful to A. G. Volosniev for the valuable discussions. We thank D. Milius for the assistance with microscopy. D. R. would like to thank F. Filakovský and T. Čuchráč for the valuable discussions. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Miba Machine Shop Facility (MS).","publication_status":"published","article_processing_charge":"Yes","OA_type":"gold","oa":1,"OA_place":"publisher","day":"16","ddc":["530"],"author":[{"first_name":"Dmytro","full_name":"Rak, Dmytro","last_name":"Rak","id":"70313b46-47c2-11ec-9e88-cd79101918fe"},{"last_name":"Lorenc","id":"40D8A3E6-F248-11E8-B48F-1D18A9856A87","first_name":"Dusan","full_name":"Lorenc, Dusan"},{"orcid":"0000-0001-7597-043X","last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","full_name":"Balazs, Daniel","first_name":"Daniel"},{"last_name":"Zhumekenov","first_name":"Ayan A.","full_name":"Zhumekenov, Ayan A."},{"first_name":"Osman M.","full_name":"Bakr, Osman M.","last_name":"Bakr"},{"last_name":"Alpichshev","id":"45E67A2A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7183-5203","full_name":"Alpichshev, Zhanybek","first_name":"Zhanybek"}]},{"abstract":[{"lang":"eng","text":"Insulating oxides are among the most abundant solid materials in the universe1,2,3. Of the many ways in which they influence natural phenomena, perhaps the most consequential is their capacity to transfer electrical charge during contact4,5,6,7,8,9,10—which occurs even between samples of the same oxide—yet the symmetry-breaking parameter that causes this remains unidentified11,12. Here we show that adventitious carbonaceous molecules adsorbed from the environment are the symmetry-breaking factor in same-material oxide contact electrification (CE). We use acoustic levitation to measure charge exchange between a sphere and a plate composed of identical amorphous silicon dioxide (SiO2). Although charging polarity is random for co-prepared samples, we control it with baking or plasma treatment. Observing the charge-exchange relaxation afterwards, we see dynamics over a timescale of hours and connect this directly to the presence of adventitious carbon with time-of-flight mass spectrometry, low-energy ion scattering and infrared spectroscopy. Going further, we confirm that adventitious carbon can even determine charge exchange among different oxides. Our results identify the symmetry-breaking parameter that causes insulating oxides to exchange charge in settings ranging from desert sands4 to volcanic plumes5,6, while simultaneously highlighting an overlooked factor in CE more broadly."}],"title":"Adventitious carbon breaks symmetry in oxide contact electrification","publication_status":"published","page":"626-631","acknowledgement":"This project has received support from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 949120) and from the Marie Skłodowska-Curie programme (grant agreement no. 754411). We acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. N.M. acknowledges support from grant Fondecyt 1221597. G.G. is a Serra Húnter fellow. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility and Lab Support Facility. We thank the Modic group for the use of the Laue camera, T. Zauner for the photography of the experimental set-up and R. Möller for insightful discussions. Open access funding provided by Institute of Science and Technology (IST Austria).","OA_place":"publisher","oa":1,"OA_type":"hybrid","article_processing_charge":"Yes (via OA deal)","issue":"8106","author":[{"full_name":"Grosjean, Galien M","first_name":"Galien M","last_name":"Grosjean","id":"0C5FDA4A-9CF6-11E9-8939-FF05E6697425","orcid":"0000-0001-5154-417X"},{"last_name":"Ostermann","full_name":"Ostermann, Markus","first_name":"Markus"},{"last_name":"Sauer","full_name":"Sauer, Markus","first_name":"Markus"},{"last_name":"Hahn","first_name":"Michael","full_name":"Hahn, Michael"},{"full_name":"Pichler, Christian M.","first_name":"Christian M.","last_name":"Pichler"},{"first_name":"Florian","full_name":"Fahrnberger, Florian","last_name":"Fahrnberger"},{"full_name":"Pertl, Felix","first_name":"Felix","last_name":"Pertl","id":"6313aec0-15b2-11ec-abd3-ed67d16139af","orcid":"0000-0003-0463-5794"},{"orcid":"0000-0001-7597-043X","last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","first_name":"Daniel","full_name":"Balazs, Daniel"},{"full_name":"Link, Mason M.","first_name":"Mason M.","last_name":"Link"},{"first_name":"Seong H.","full_name":"Kim, Seong H.","last_name":"Kim"},{"last_name":"Schrader","full_name":"Schrader, Devin L.","first_name":"Devin L."},{"last_name":"Blanco","first_name":"Adriana","full_name":"Blanco, Adriana"},{"full_name":"Gracia, Francisco","first_name":"Francisco","last_name":"Gracia"},{"full_name":"Mujica, Nicolás","first_name":"Nicolás","last_name":"Mujica"},{"first_name":"Scott R","full_name":"Waitukaitis, Scott R","orcid":"0000-0002-2299-3176","id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","last_name":"Waitukaitis"}],"day":"18","ddc":["540"],"pmid":1,"external_id":{"pmid":["41851325"]},"language":[{"iso":"eng"}],"year":"2026","article_type":"original","publication":"Nature","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"file":[{"date_created":"2026-03-24T06:57:08Z","file_size":12245694,"date_updated":"2026-03-24T06:57:08Z","success":1,"creator":"dernst","access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_id":"21494","file_name":"2026_Nature_Grosjean.pdf","checksum":"dafef9ed575b44be4263e948a47ae056"}],"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"_id":"21485","month":"03","oa_version":"Published Version","date_updated":"2026-04-28T12:06:01Z","quality_controlled":"1","volume":651,"date_created":"2026-03-23T15:04:00Z","project":[{"name":"Tribocharge: a multi-scale approach to an enduring problem in physics","_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","grant_number":"949120","call_identifier":"H2020"},{"call_identifier":"H2020","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships"}],"doi":"10.1038/s41586-025-10088-w","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"PlanS_conform":"1","has_accepted_license":"1","type":"journal_article","related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/colliding-dust-and-the-sparks-of-creation/"}]},"citation":{"mla":"Grosjean, Galien M., et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” <i>Nature</i>, vol. 651, no. 8106, Springer Nature, 2026, pp. 626–31, doi:<a href=\"https://doi.org/10.1038/s41586-025-10088-w\">10.1038/s41586-025-10088-w</a>.","chicago":"Grosjean, Galien M, Markus Ostermann, Markus Sauer, Michael Hahn, Christian M. Pichler, Florian Fahrnberger, Felix Pertl, et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” <i>Nature</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41586-025-10088-w\">https://doi.org/10.1038/s41586-025-10088-w</a>.","short":"G.M. Grosjean, M. Ostermann, M. Sauer, M. Hahn, C.M. Pichler, F. Fahrnberger, F. Pertl, D. Balazs, M.M. Link, S.H. Kim, D.L. Schrader, A. Blanco, F. Gracia, N. Mujica, S.R. Waitukaitis, Nature 651 (2026) 626–631.","ista":"Grosjean GM, Ostermann M, Sauer M, Hahn M, Pichler CM, Fahrnberger F, Pertl F, Balazs D, Link MM, Kim SH, Schrader DL, Blanco A, Gracia F, Mujica N, Waitukaitis SR. 2026. Adventitious carbon breaks symmetry in oxide contact electrification. Nature. 651(8106), 626–631.","apa":"Grosjean, G. M., Ostermann, M., Sauer, M., Hahn, M., Pichler, C. M., Fahrnberger, F., … Waitukaitis, S. R. (2026). Adventitious carbon breaks symmetry in oxide contact electrification. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-10088-w\">https://doi.org/10.1038/s41586-025-10088-w</a>","ieee":"G. M. Grosjean <i>et al.</i>, “Adventitious carbon breaks symmetry in oxide contact electrification,” <i>Nature</i>, vol. 651, no. 8106. Springer Nature, pp. 626–631, 2026.","ama":"Grosjean GM, Ostermann M, Sauer M, et al. Adventitious carbon breaks symmetry in oxide contact electrification. <i>Nature</i>. 2026;651(8106):626-631. doi:<a href=\"https://doi.org/10.1038/s41586-025-10088-w\">10.1038/s41586-025-10088-w</a>"},"date_published":"2026-03-18T00:00:00Z","intvolume":"       651","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","status":"public","corr_author":"1","ec_funded":1,"file_date_updated":"2026-03-24T06:57:08Z","department":[{"_id":"ScWa"},{"_id":"GradSch"},{"_id":"LifeSc"}],"publisher":"Springer Nature"},{"date_published":"2026-06-09T00:00:00Z","citation":{"apa":"Wu, J., Corvaglia, V., Chakrabortty, T., Mandal, P. K., &#38; Huc, I. (n.d.). Tailoring the major groove of DNA mimic foldamers. <i>Chemical Science</i>. Royal Society of Chemistry. <a href=\"https://doi.org/10.1039/d6sc00798h\">https://doi.org/10.1039/d6sc00798h</a>","ieee":"J. Wu, V. Corvaglia, T. Chakrabortty, P. K. Mandal, and I. Huc, “Tailoring the major groove of DNA mimic foldamers,” <i>Chemical Science</i>. Royal Society of Chemistry.","ama":"Wu J, Corvaglia V, Chakrabortty T, Mandal PK, Huc I. Tailoring the major groove of DNA mimic foldamers. <i>Chemical Science</i>. doi:<a href=\"https://doi.org/10.1039/d6sc00798h\">10.1039/d6sc00798h</a>","mla":"Wu, Jiaojiao, et al. “Tailoring the Major Groove of DNA Mimic Foldamers.” <i>Chemical Science</i>, Royal Society of Chemistry, doi:<a href=\"https://doi.org/10.1039/d6sc00798h\">10.1039/d6sc00798h</a>.","chicago":"Wu, Jiaojiao, Valentina Corvaglia, Tulika Chakrabortty, Pradeep K Mandal, and Ivan Huc. “Tailoring the Major Groove of DNA Mimic Foldamers.” <i>Chemical Science</i>. Royal Society of Chemistry, n.d. <a href=\"https://doi.org/10.1039/d6sc00798h\">https://doi.org/10.1039/d6sc00798h</a>.","short":"J. Wu, V. Corvaglia, T. Chakrabortty, P.K. Mandal, I. Huc, Chemical Science (n.d.).","ista":"Wu J, Corvaglia V, Chakrabortty T, Mandal PK, Huc I. Tailoring the major groove of DNA mimic foldamers. Chemical Science."},"DOAJ_listed":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","department":[{"_id":"LifeSc"}],"publisher":"Royal Society of Chemistry","quality_controlled":"1","date_created":"2026-07-13T09:41:36Z","date_updated":"2026-07-13T11:24:29Z","_id":"22289","month":"06","oa_version":"Published Version","scopus_import":"1","doi":"10.1039/d6sc00798h","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","type":"journal_article","supplementarymaterial":"yes","researchdata_availability":"yes","language":[{"iso":"eng"}],"dataavailabilitystatement":"CCDC 2514117, 2514118, 2286782 and 2478322 (compound 1, compound 1d, oligomer 5, and oligomer 6, respectively) contain the supplementary crystallographic data for this paper.54a–d \r\n\r\nThe supporting data have been provided as part of the supplementary information (SI). Supplementary information: SI figures, detailed experimental protocols, crystallographic studies, and characterisation of new compounds. See DOI: https://doi.org/10.1039/d6sc00798h.","article_type":"original","year":"2026","publication":"Chemical Science","das_tickbox":"1","publication_identifier":{"eissn":["2041-6539"],"issn":["2041-6520"]},"abstract":[{"lang":"eng","text":"Single-stranded, helically folded aromatic oligoamides bearing anionic phosphonate side chains have been shown to bind to some DNA-binding proteins better than DNA itself. However, these DNA mimic foldamers have until now mainly consisted of a single repeat motif, like a poly(dA:dT) DNA duplex, and contained limited sequence information. Here, we introduce new monomers designed to display different chemical functionalities in the major groove of the DNA mimics. Four new Fmoc-protected amino acid monomers have been synthesized and incorporated into oligomers. Sixteen foldamer sequences were prepared on solid phase. Their conformations in solution and in the solid state and their conformational dynamics were investigated using nuclear magnetic resonance, circular dichroism, molecular modeling, and X-ray crystallography. The results show that three of the four new monomers behaved as designed and that their introduction enhances the conformational dynamics of the DNA mimic foldamers. In a fourth case, conformational behavior proved to be more complex than expected. The modified sequences retained the ability to bind to the bacterial histone-like protein HU. These results showcase design strategies to manipulate large molecular biomimetics in which not only side chains but also main chain components are varied. The new monomers pave the way to complex DNA mimic foldamer sequences targeting proteins that recognize sequence-selective DNA-binding proteins such as transcription factors or restriction enzymes."}],"title":"Tailoring the major groove of DNA mimic foldamers","publication_status":"inpress","acknowledgement":"We acknowledge financial support from the European Research Council (ERC) under the European Union's Horizon Europe Framework Programme (grant agreement no. ERC-2021-ADG-320892) and from the China Scholarship Council (CSC, predoctoral fellowship to J. W.). We thank L. Allmendinger for assistance with NMR measurements, P. Mayer for his assistance in solving the crystal structures of 1 and 1d, L. Bodero for assistance with automated solid-phase synthesis, M. Rogovoi for providing monomer precursors, and M. Loos for the purification and analysis of compounds 15a–19a. We thank M. Soler-Lopez (ID23-1, ESRF, Grenoble) and I. Bento (EMBL P13, Petra III, DESY, Hamburg) for assistance during data collection at the synchrotron beamlines.","OA_place":"publisher","article_processing_charge":"Yes","OA_type":"gold","ddc":["540"],"day":"09","author":[{"last_name":"Wu","first_name":"Jiaojiao","full_name":"Wu, Jiaojiao"},{"full_name":"Corvaglia, Valentina","first_name":"Valentina","last_name":"Corvaglia"},{"full_name":"Chakrabortty, Tulika","first_name":"Tulika","last_name":"Chakrabortty"},{"id":"6a3def15-d4b4-11ef-9fa9-a24c1f545ec3","last_name":"Mandal","orcid":"0000-0001-5996-956X","first_name":"Pradeep K","full_name":"Mandal, Pradeep K"},{"last_name":"Huc","first_name":"Ivan","full_name":"Huc, Ivan"}]},{"quality_controlled":"1","date_updated":"2026-07-20T09:49:12Z","date_created":"2026-06-21T22:03:01Z","month":"06","_id":"22105","oa_version":"Published Version","project":[{"name":"Exploring protein dynamics by solid-state MAS NMR through specific labeling approaches","_id":"7be609c4-9f16-11ee-852c-85015ce2b9b0","grant_number":"26777"}],"scopus_import":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41557-026-02155-0"}],"doi":"10.1038/s41557-026-02155-0","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"PlanS_conform":"1","type":"journal_article","related_material":{"record":[{"status":"public","id":"20641","relation":"research_data"},{"id":"21145","relation":"research_data","status":"public"},{"status":"public","id":"22334","relation":"dissertation_contains"}]},"supplementarymaterial":"yes","date_published":"2026-06-10T00:00:00Z","citation":{"apa":"Becker, L. M., Fu, H., Tatman, B., Dreydoppel, M., Kapitonova, A., Balazs, D., … Schanda, P. (2026). Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes. <i>Nature Chemistry</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41557-026-02155-0\">https://doi.org/10.1038/s41557-026-02155-0</a>","ieee":"L. M. Becker <i>et al.</i>, “Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes,” <i>Nature Chemistry</i>. Springer Nature, 2026.","ama":"Becker LM, Fu H, Tatman B, et al. Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes. <i>Nature Chemistry</i>. 2026. doi:<a href=\"https://doi.org/10.1038/s41557-026-02155-0\">10.1038/s41557-026-02155-0</a>","mla":"Becker, Lea Marie, et al. “Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.” <i>Nature Chemistry</i>, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41557-026-02155-0\">10.1038/s41557-026-02155-0</a>.","chicago":"Becker, Lea Marie, Haohao Fu, Benjamin Tatman, Matthias Dreydoppel, Anna Kapitonova, Daniel Balazs, Ulrich Weininger, Sylvain Engilberge, Christophe Chipot, and Paul Schanda. “Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.” <i>Nature Chemistry</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41557-026-02155-0\">https://doi.org/10.1038/s41557-026-02155-0</a>.","short":"L.M. Becker, H. Fu, B. Tatman, M. Dreydoppel, A. Kapitonova, D. Balazs, U. Weininger, S. Engilberge, C. Chipot, P. Schanda, Nature Chemistry (2026).","ista":"Becker LM, Fu H, Tatman B, Dreydoppel M, Kapitonova A, Balazs D, Weininger U, Engilberge S, Chipot C, Schanda P. 2026. Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes. Nature Chemistry."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","status":"public","department":[{"_id":"PaSc"},{"_id":"LifeSc"}],"publisher":"Springer Nature","abstract":[{"text":"Protein conformational energy landscapes are shaped not only by intramolecular interactions but also by their environment. In protein crystals and protein–protein complexes, intermolecular contacts alter this energy landscape, but the exact nature of this alteration is difficult to decipher. Understanding how the crystal lattice affects protein dynamics is crucial for crystallography-based studies of motion, yet its influence on collective motions remains unclear. Aromatic ring flips in the hydrophobic core represent sensitive probes of such dynamics. Here, we compare the kinetics of aromatic ring flips in the protein GB1 in crystals, in complex with its binding partner IgG, and in solution, combining advanced isotope labelling with quantitative NMR methods. We show that rings in the core flip nearly a thousand times less frequently in crystals than in solution. Enhanced-sampling molecular dynamics simulations, based on a crystal structure of a GB1 variant reported in this work, reproduce these elevated barriers and reveal how the crystal restrains motions.","lang":"eng"}],"title":"Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes","publication_status":"epub_ahead","acknowledgement":"We thank N. R. Skrynnikov and O. O. Lebedenko (St. Petersburg) for insightful discussions and for performing exploratory MD simulations. We are grateful to T. Schubeis (Lyon) for advice on GB1 crystallization and R. Schmid for initial crystallization trials. We thank C. Mueller-Dieckmann for assistance with room-temperature X-ray crystallography data collection on beamline ID30B at the ESRF, which is acknowledged for providing beamtime through its In-House Research programme. We thank S. Falkner for assistance with constructing the structural model of the IgG:GB1 complex. We thank J. Lewandowski for providing feedback on the paper and granting access to backbone relaxation data of IgG:GB1T2Q and GB1T2Q microcrystals. This research was supported by the Scientific Service Units (SSU) of the Institute of Science and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance and the Lab Support Facilities. We thank P. Rovó and M. V. Falcón for excellent support of the NMR facility. L.M.B. is recipient of a DOC fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology Austria (grant number PR10660EAW01). C.C. acknowledges the European Research Council (grant project 101097272 ‘MilliInMicro’) and the Métropole du Grand Nancy (grant project ‘ARC’). BM07-FIP2 is supported by the French ANR PIA3 (France 2030) EquipEx+ project MAGNIFIX under grant agreement ANR-21-ESRE-0011.Open access funding provided by Institute of Science and Technology (IST Austria).","oa":1,"OA_place":"publisher","article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","day":"10","ddc":["540"],"author":[{"full_name":"Becker, Lea Marie","first_name":"Lea Marie","orcid":"0000-0002-6401-5151","id":"36336939-eb97-11eb-a6c2-c83f1214ca79","last_name":"Becker"},{"last_name":"Fu","full_name":"Fu, Haohao","first_name":"Haohao"},{"id":"71cda2f3-e604-11ee-a1df-da10587eda3f","last_name":"Tatman","full_name":"Tatman, Benjamin","first_name":"Benjamin"},{"last_name":"Dreydoppel","full_name":"Dreydoppel, Matthias","first_name":"Matthias"},{"last_name":"Kapitonova","id":"9fb2a840-89e1-11ee-a8b7-cc5c7ba62471","full_name":"Kapitonova, Anna","first_name":"Anna"},{"last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","orcid":"0000-0001-7597-043X","first_name":"Daniel","full_name":"Balazs, Daniel"},{"full_name":"Weininger, Ulrich","first_name":"Ulrich","last_name":"Weininger"},{"full_name":"Engilberge, Sylvain","first_name":"Sylvain","last_name":"Engilberge"},{"first_name":"Christophe","full_name":"Chipot, Christophe","last_name":"Chipot"},{"full_name":"Schanda, Paul","first_name":"Paul","last_name":"Schanda","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","orcid":"0000-0002-9350-7606"}],"researchdata_availability":"yes","language":[{"iso":"eng"}],"pmid":1,"external_id":{"pmid":["42271006"]},"dataavailabilitystatement":"The cryo and room-temperature crystal structures of GB1QDD are deposited at the PDB under the access codes 9I2I and 9T8Z, respectively. The solid-state NMR backbone assignment of GB1QDD is deposited at the BMRB under the access code 53330. NMR spectra, analysis scripts and raw data are publicly available at the ISTA research explorer (https://doi.org/10.15479/AT-ISTA-20641)120. Files to reproduce the enhanced-sampling MD simulations are publicly available at the ISTA research explorer (https://doi.org/10.15479/AT-ISTA-21145)121.","article_type":"original","year":"2026","publication":"Nature Chemistry","das_tickbox":"1","publication_identifier":{"issn":["17554330"],"eissn":["17554349"]},"acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}]},{"publisher":"Springer Nature","department":[{"_id":"SyCr"},{"_id":"LifeSc"}],"file_date_updated":"2025-12-15T13:30:33Z","ec_funded":1,"corr_author":"1","status":"public","article_number":"10511","date_published":"2025-12-01T00:00:00Z","citation":{"ama":"Dawson E, Hönigsberger M, Kampleitner N, et al. Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>","ieee":"E. Dawson <i>et al.</i>, “Altruistic disease signalling in ant colonies,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","apa":"Dawson, E., Hönigsberger, M., Kampleitner, N., Grasse, A. V., Lindorfer, L., Robb, J., … Cremer, S. (2025). Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>","ista":"Dawson E, Hönigsberger M, Kampleitner N, Grasse AV, Lindorfer L, Robb J, Beikzadeh F, Strahodinsky F, Leitner H, Rajendran H, Schmitt T, Cremer S. 2025. Altruistic disease signalling in ant colonies. Nature Communications. 16, 10511.","short":"E. Dawson, M. Hönigsberger, N. Kampleitner, A.V. Grasse, L. Lindorfer, J. Robb, F. Beikzadeh, F. Strahodinsky, H. Leitner, H. Rajendran, T. Schmitt, S. Cremer, Nature Communications 16 (2025).","chicago":"Dawson, Erika, Michaela Hönigsberger, Niklas Kampleitner, Anna V Grasse, Lukas Lindorfer, Jennifer Robb, Farnaz Beikzadeh, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>.","mla":"Dawson, Erika, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>, vol. 16, 10511, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>."},"DOAJ_listed":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"        16","related_material":{"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/ants-signal-deadly-infection/"}],"record":[{"status":"public","relation":"research_data","id":"20471"}]},"has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"PlanS_conform":"1","type":"journal_article","main_file_link":[{"url":"https://doi.org/10.1101/2024.02.27.582277","open_access":"1"}],"doi":"10.1038/s41467-025-66175-z","date_updated":"2026-06-10T08:50:53Z","date_created":"2025-01-27T11:28:05Z","quality_controlled":"1","volume":16,"_id":"18892","oa_version":"Published Version","month":"12","project":[{"call_identifier":"H2020","grant_number":"771402","_id":"2649B4DE-B435-11E9-9278-68D0E5697425","name":"Epidemics in ant societies on a chip"},{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"}],"scopus_import":"1","publication_identifier":{"eissn":["2041-1723"]},"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"MassSpec"}],"file":[{"success":1,"creator":"dernst","date_updated":"2025-12-15T13:30:33Z","date_created":"2025-12-15T13:30:33Z","file_size":805323,"content_type":"application/pdf","access_level":"open_access","file_name":"2025_NatureComm_Dawson.pdf","file_id":"20826","relation":"main_file","checksum":"06244623bb7611c636652ecbc4787889"}],"publication":"Nature Communications","article_type":"original","year":"2025","language":[{"iso":"eng"}],"external_id":{"pmid":["41330896"]},"pmid":1,"ddc":["570"],"day":"01","author":[{"full_name":"Dawson, Erika","first_name":"Erika","id":"31B4E2D0-F248-11E8-B48F-1D18A9856A87","last_name":"Dawson"},{"last_name":"Hönigsberger","id":"953894f3-25bd-11ec-8556-f70a9d38ef60","first_name":"Michaela","full_name":"Hönigsberger, Michaela"},{"first_name":"Niklas","full_name":"Kampleitner, Niklas","id":"2AC57FAC-F248-11E8-B48F-1D18A9856A87","last_name":"Kampleitner"},{"full_name":"Grasse, Anna V","first_name":"Anna V","id":"406F989C-F248-11E8-B48F-1D18A9856A87","last_name":"Grasse"},{"id":"85f0e6d3-06b3-11ec-8982-8c5049fa4455","last_name":"Lindorfer","full_name":"Lindorfer, Lukas","first_name":"Lukas"},{"first_name":"Jennifer","full_name":"Robb, Jennifer","id":"7bc2734a-e2c6-11ea-9824-a2ed5f0662a8","last_name":"Robb"},{"first_name":"Farnaz","full_name":"Beikzadeh Abbasi, Farnaz","id":"0344bfb9-3feb-11ee-87e9-c27edc800bcd","last_name":"Beikzadeh Abbasi"},{"full_name":"Strahodinsky, Florian","first_name":"Florian","id":"979E35EE-C996-11E9-8C7C-CF13E6697425","last_name":"Strahodinsky"},{"id":"8fc5c6f6-5903-11ec-abad-c83f046253e7","last_name":"Leitner","full_name":"Leitner, Hanna","first_name":"Hanna"},{"full_name":"Rajendran, Harikrishnan","first_name":"Harikrishnan","id":"876b6b34-8ff4-11ec-97c9-8d95a7aae416","last_name":"Rajendran"},{"full_name":"Schmitt, Thomas","first_name":"Thomas","last_name":"Schmitt"},{"id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","last_name":"Cremer","orcid":"0000-0002-2193-3868","full_name":"Cremer, Sylvia","first_name":"Sylvia"}],"OA_place":"publisher","oa":1,"article_processing_charge":"Yes","OA_type":"gold","publication_status":"published","acknowledgement":"We thank Joergen Eilenberg and Nicolai V. Meyling for the fungal strain, and the ISTA Social Immunity team, Jonghyun Park and Yuko Ulrich for ant collection. We also thank the Social Immunity team, in particular David Moreno Martínez, Tanvi Madaan, Wilfrid Jean Louis and Jessica Kirchner, for experimental and molecular support, as well as Friedrich Fochler for technical support with the chemical analysis, and the ISTA Lab Support Facility, including the mass spectrometry unit, for general and chemical laboratory support. We further thank Marco Ribezzi for advice on 13C calculations and Ernst Pittenauer for discussion of the chemical data, Chris Pull and Michael Sixt for project discussion, and the Social Immunity team for comments on the manuscript. The study was funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation Programme (No. 771402; EPIDEMICSonCHIP) to SC.","APC_amount":"7068 EUR","abstract":[{"lang":"eng","text":"Sick individuals often conceal their disease status to group members, thereby preventing social exclusion or aggression. Here we show by behavioural, chemical, immunological and infection load analyses that sick ant pupae instead actively emit a chemical signal that in itself is sufficient to trigger their own destruction by colony members. In our experiments, this altruistic disease-signalling was performed only by worker but not queen pupae. The lack of signalling by queen pupae did not constitute cheating behaviour, but reflected their superior immune capabilities. Worker pupae suffered from extensive pathogen replication whereas queen pupae were able to restrain their infection. Our data suggest the evolution of a finely-tuned signalling system in which it is not the induction of an individual’s immune response, but rather its failure to overcome the infection, that triggers pupal signalling for sacrifice. This demonstrates a balanced interplay between individual and social immunity that efficiently achieves whole-colony health."}],"title":"Altruistic disease signalling in ant colonies"},{"publication":"Nature","file":[{"content_type":"application/pdf","access_level":"open_access","success":1,"creator":"dernst","date_created":"2025-03-04T10:05:18Z","file_size":3807415,"date_updated":"2025-03-04T10:05:18Z","checksum":"fecf302274dd3218d3e7dd22f39a6c0c","file_name":"2025_Nature_Sobarzo.pdf","file_id":"19289","relation":"main_file"}],"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"isi":1,"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"external_id":{"pmid":["39972227"],"isi":["001428076100015"]},"pmid":1,"language":[{"iso":"eng"}],"year":"2025","article_type":"original","OA_type":"hybrid","article_processing_charge":"Yes (via OA deal)","OA_place":"publisher","oa":1,"author":[{"last_name":"Sobarzo Ponce","id":"4B807D68-AE37-11E9-AC72-31CAE5697425","first_name":"Juan Carlos A","full_name":"Sobarzo Ponce, Juan Carlos A"},{"id":"6313aec0-15b2-11ec-abd3-ed67d16139af","last_name":"Pertl","orcid":"0000-0003-0463-5794","full_name":"Pertl, Felix","first_name":"Felix"},{"first_name":"Daniel","full_name":"Balazs, Daniel","last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","orcid":"0000-0001-7597-043X"},{"last_name":"Costanzo","id":"D93824F4-D9BA-11E9-BB12-F207E6697425","orcid":"0000-0001-9732-3815","full_name":"Costanzo, Tommaso","first_name":"Tommaso"},{"last_name":"Sauer","first_name":"Markus","full_name":"Sauer, Markus"},{"full_name":"Foelske, Annette","first_name":"Annette","last_name":"Foelske"},{"full_name":"Ostermann, Markus","first_name":"Markus","last_name":"Ostermann"},{"last_name":"Pichler","first_name":"Christian M.","full_name":"Pichler, Christian M."},{"last_name":"Wang","full_name":"Wang, Yongkang","first_name":"Yongkang"},{"last_name":"Nagata","first_name":"Yuki","full_name":"Nagata, Yuki"},{"last_name":"Bonn","full_name":"Bonn, Mischa","first_name":"Mischa"},{"orcid":"0000-0002-2299-3176","id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","last_name":"Waitukaitis","first_name":"Scott R","full_name":"Waitukaitis, Scott R"}],"ddc":["530"],"day":"20","issue":"8051","title":"Spontaneous ordering of identical materials into a triboelectric series","abstract":[{"lang":"eng","text":"When two insulating, neutral materials are contacted and separated, they exchange electrical charge1. Experiments have long suggested that this ‘contact electrification’ is transitive, with different materials ordering into ‘triboelectric series’ based on the sign of charge acquired2. At the same time, the effect is plagued by unpredictability, preventing consensus on the mechanism and casting doubt on the rhyme and reason that series imply3. Here we expose an unanticipated connection between the unpredictability and order in contact electrification: nominally identical materials initially exchange charge randomly and intransitively, but—over repeated experiments—order into triboelectric series. We find that this evolution is driven by the act of contact itself—samples with more contacts in their history charge negatively to ones with fewer contacts. Capturing this ‘contact bias’ in a minimal model, we recreate both the initial randomness and ultimate order in numerical simulations and use it experimentally to force the appearance of a triboelectric series of our choosing. With a set of surface-sensitive techniques to search for the underlying alterations contact creates, we only find evidence of nanoscale morphological changes, pointing to a mechanism strongly coupled with mechanics. Our results highlight the centrality of contact history in contact electrification and suggest that focusing on the unpredictability that has long plagued the effect may hold the key to understanding it."}],"acknowledgement":"This project has received financing from the European Research Council grant agreement no. 949120 under the European Union’s Horizon 2020 research and innovation programme. The Analytical Instrumentation Center of the TU Wien acknowledges support by the FFG project ‘ELSA’ under grant no. 884672. C.M.P. and M.O. acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility, Electron Microscopy Facility and Lab Support Facility. We thank J. Garcia-Suarez and G. Anciaux for the suggestion to look into the roughness power spectral density. We thank I.-M. Strugaru for help with testing the device for Young’s modulus measurements. Open access funding provided by Institute of Science and Technology (IST Austria).","publication_status":"published","file_date_updated":"2025-03-04T10:05:18Z","department":[{"_id":"ScWa"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"publisher":"Springer Nature","intvolume":"       638","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_published":"2025-02-20T00:00:00Z","citation":{"ieee":"J. C. A. Sobarzo Ponce <i>et al.</i>, “Spontaneous ordering of identical materials into a triboelectric series,” <i>Nature</i>, vol. 638, no. 8051. Springer Nature, 2025.","apa":"Sobarzo Ponce, J. C. A., Pertl, F., Balazs, D., Costanzo, T., Sauer, M., Foelske, A., … Waitukaitis, S. R. (2025). Spontaneous ordering of identical materials into a triboelectric series. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-024-08530-6\">https://doi.org/10.1038/s41586-024-08530-6</a>","ama":"Sobarzo Ponce JCA, Pertl F, Balazs D, et al. Spontaneous ordering of identical materials into a triboelectric series. <i>Nature</i>. 2025;638(8051). doi:<a href=\"https://doi.org/10.1038/s41586-024-08530-6\">10.1038/s41586-024-08530-6</a>","short":"J.C.A. Sobarzo Ponce, F. Pertl, D. Balazs, T. Costanzo, M. Sauer, A. Foelske, M. Ostermann, C.M. Pichler, Y. Wang, Y. Nagata, M. Bonn, S.R. Waitukaitis, Nature 638 (2025).","chicago":"Sobarzo Ponce, Juan Carlos A, Felix Pertl, Daniel Balazs, Tommaso Costanzo, Markus Sauer, Annette Foelske, Markus Ostermann, et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-024-08530-6\">https://doi.org/10.1038/s41586-024-08530-6</a>.","mla":"Sobarzo Ponce, Juan Carlos A., et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” <i>Nature</i>, vol. 638, no. 8051, 664–669, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41586-024-08530-6\">10.1038/s41586-024-08530-6</a>.","ista":"Sobarzo Ponce JCA, Pertl F, Balazs D, Costanzo T, Sauer M, Foelske A, Ostermann M, Pichler CM, Wang Y, Nagata Y, Bonn M, Waitukaitis SR. 2025. Spontaneous ordering of identical materials into a triboelectric series. Nature. 638(8051), 664–669."},"article_number":"664-669","corr_author":"1","status":"public","ec_funded":1,"type":"journal_article","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","related_material":{"link":[{"url":"https://ista.ac.at/en/news/an-electrifying-turn-in-an-age-old-quest/","relation":"press_release","description":"News on ISTA website"}],"record":[{"relation":"dissertation_contains","id":"20203","status":"public"}]},"scopus_import":"1","project":[{"grant_number":"949120","call_identifier":"H2020","name":"Tribocharge: a multi-scale approach to an enduring problem in physics","_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa"}],"_id":"19278","oa_version":"Published Version","month":"02","volume":638,"date_created":"2025-03-02T23:01:52Z","date_updated":"2026-04-28T13:44:56Z","quality_controlled":"1","doi":"10.1038/s41586-024-08530-6"},{"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"NMR"},{"_id":"LifeSc"}],"publisher":"Fundació de la comunitat valenciana SCITO","department":[{"_id":"MaIb"},{"_id":"LifeSc"}],"publication":"Proceedings of the MATSUS Spring 2025 Conference","year":"2025","article_number":"173","corr_author":"1","status":"public","language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2025-03-15T00:00:00Z","citation":{"ama":"Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. In: <i>Proceedings of the MATSUS Spring 2025 Conference</i>. Fundació de la comunitat valenciana SCITO; 2025. doi:<a href=\"https://doi.org/10.29363/nanoge.matsusspring.2025.173\">10.29363/nanoge.matsusspring.2025.173</a>","apa":"Lee, S., Balazs, D., Horta, S., Rayaroth Puthiyaveettil, A., &#38; Ibáñez, M. (2025). Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. In <i>Proceedings of the MATSUS Spring 2025 Conference</i>. Sevilla, Spain: Fundació de la comunitat valenciana SCITO. <a href=\"https://doi.org/10.29363/nanoge.matsusspring.2025.173\">https://doi.org/10.29363/nanoge.matsusspring.2025.173</a>","ieee":"S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, and M. Ibáñez, “Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case,” in <i>Proceedings of the MATSUS Spring 2025 Conference</i>, Sevilla, Spain, 2025.","ista":"Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. 2025. Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. Proceedings of the MATSUS Spring 2025 Conference. MATSUS: Materials for Sustainable Development Conference, 173.","mla":"Lee, Seungho, et al. “Reaction Precursor-Mediated Formation of Stable Supercrystals in Colloidal Nanocrystal Synthesis: PbTe Case.” <i>Proceedings of the MATSUS Spring 2025 Conference</i>, 173, Fundació de la comunitat valenciana SCITO, 2025, doi:<a href=\"https://doi.org/10.29363/nanoge.matsusspring.2025.173\">10.29363/nanoge.matsusspring.2025.173</a>.","chicago":"Lee, Seungho, Daniel Balazs, Sharona Horta, Aiswarya Rayaroth Puthiyaveettil, and Maria Ibáñez. “Reaction Precursor-Mediated Formation of Stable Supercrystals in Colloidal Nanocrystal Synthesis: PbTe Case.” In <i>Proceedings of the MATSUS Spring 2025 Conference</i>. Fundació de la comunitat valenciana SCITO, 2025. <a href=\"https://doi.org/10.29363/nanoge.matsusspring.2025.173\">https://doi.org/10.29363/nanoge.matsusspring.2025.173</a>.","short":"S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, M. Ibáñez, in:, Proceedings of the MATSUS Spring 2025 Conference, Fundació de la comunitat valenciana SCITO, 2025."},"day":"15","author":[{"full_name":"Lee, Seungho","first_name":"Seungho","id":"BB243B88-D767-11E9-B658-BC13E6697425","last_name":"Lee","orcid":"0000-0002-6962-8598"},{"orcid":"0000-0001-7597-043X","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","last_name":"Balazs","first_name":"Daniel","full_name":"Balazs, Daniel"},{"last_name":"Horta","id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc","full_name":"Horta, Sharona","first_name":"Sharona"},{"full_name":"Rayaroth Puthiyaveettil, Aiswarya","first_name":"Aiswarya","id":"8aceb01b-8972-11ed-ae7b-d5fe53775add","last_name":"Rayaroth Puthiyaveettil"},{"first_name":"Maria","full_name":"Ibáñez, Maria","orcid":"0000-0001-5013-2843","last_name":"Ibáñez","id":"43C61214-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"No","OA_type":"closed access","type":"conference","conference":{"end_date":"2025-03-07","name":"MATSUS: Materials for Sustainable Development Conference","location":"Sevilla, Spain","start_date":"2025-03-03"},"acknowledgement":"ISTA and the Werner Siemens Foundation financially supported this work. The Scientific Service Units (SSU) of ISTA supported this research through resources provided by the Electron Microscopy Facility (EMF), NMR Facility and the Lab Support Facility (LSF).","doi":"10.29363/nanoge.matsusspring.2025.173","publication_status":"published","title":"Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case","project":[{"_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A","name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery"}],"date_updated":"2026-02-19T09:25:57Z","quality_controlled":"1","abstract":[{"lang":"eng","text":"Supercrystals represent three-dimensional orderings of colloidal nanocrystals (NCs), showcasing collective properties in photonics, phononics, and electronics applications.1,2 Recent studies have shown that such assemblies are directly produced during nanocrystal reactions.3–6 However, a fundamental understanding of in situ formed supercrystals that withstand typical NC purification processes remains underexplored, which is important for further use. Herein, we report the reaction precursor-mediated formation of stable PbTe supercrystals. Rationalizing the formation of these assemblies through small-angle x-ray scattering (SAXS) measurements, we unveil their formation mechanism. Our findings reveal that the supercrystal formation occurs in the presence of an excess of lead oleates in the crude solution. It should be noted that the formed supercrystals can be stabilized under specific conditions determined by the lead oleate cluster concentration, content of trioctylphosphine telluride (TOP-Te), NC size and the need of an annealing step at mild conditions. Furthermore, this approach allows for the continuous growth of a secondary phase within the supercrystal; for example in the case of PbTe supercrystals, a PbS shell can be grown on each PbTe NC constituent, resulting in core-shell PbTe-PbS supercrystals. Our work elucidates that reaction precursors play an important role in in situ SC formation and stabilization, implying the possibility of applying this knowledge to other NC reactions."}],"date_created":"2025-07-21T08:33:20Z","_id":"20055","oa_version":"None","month":"03"},{"status":"public","article_number":"434","citation":{"ieee":"D. Marolt Presen <i>et al.</i>, “Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium,” <i>Stem Cell Research and Therapy</i>, vol. 15. Springer Nature, 2024.","apa":"Marolt Presen, D., Goeschl, V., Hanetseder, D., Ogrin, L., Stetco, A. L., Tansek, A., … Redl, H. (2024). Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium. <i>Stem Cell Research and Therapy</i>. Springer Nature. <a href=\"https://doi.org/10.1186/s13287-024-03960-5\">https://doi.org/10.1186/s13287-024-03960-5</a>","ama":"Marolt Presen D, Goeschl V, Hanetseder D, et al. Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium. <i>Stem Cell Research and Therapy</i>. 2024;15. doi:<a href=\"https://doi.org/10.1186/s13287-024-03960-5\">10.1186/s13287-024-03960-5</a>","chicago":"Marolt Presen, Darja, Vanessa Goeschl, Dominik Hanetseder, Laura Ogrin, Alexandra Larissa Stetco, Anja Tansek, Laura Pozenel, et al. “Prolonged Cultivation Enhances the Stimulatory Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned Medium.” <i>Stem Cell Research and Therapy</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1186/s13287-024-03960-5\">https://doi.org/10.1186/s13287-024-03960-5</a>.","short":"D. Marolt Presen, V. Goeschl, D. Hanetseder, L. Ogrin, A.L. Stetco, A. Tansek, L. Pozenel, B. Bruszel, G. Mitulovic, J. Oesterreicher, J. Zipperle, B. Schaedl, W. Holnthoner, J. Grillari, H. Redl, Stem Cell Research and Therapy 15 (2024).","mla":"Marolt Presen, Darja, et al. “Prolonged Cultivation Enhances the Stimulatory Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned Medium.” <i>Stem Cell Research and Therapy</i>, vol. 15, 434, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1186/s13287-024-03960-5\">10.1186/s13287-024-03960-5</a>.","ista":"Marolt Presen D, Goeschl V, Hanetseder D, Ogrin L, Stetco AL, Tansek A, Pozenel L, Bruszel B, Mitulovic G, Oesterreicher J, Zipperle J, Schaedl B, Holnthoner W, Grillari J, Redl H. 2024. Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium. Stem Cell Research and Therapy. 15, 434."},"date_published":"2024-12-01T00:00:00Z","intvolume":"        15","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","DOAJ_listed":"1","publisher":"Springer Nature","file_date_updated":"2024-12-10T08:28:17Z","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","department":[{"_id":"LifeSc"}],"doi":"10.1186/s13287-024-03960-5","_id":"18581","oa_version":"Published Version","month":"12","date_updated":"2025-09-09T11:41:12Z","date_created":"2024-11-24T23:01:47Z","volume":15,"quality_controlled":"1","scopus_import":"1","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"has_accepted_license":"1","type":"journal_article","year":"2024","article_type":"original","external_id":{"pmid":["39551765"],"isi":["001356479400001"]},"pmid":1,"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1757-6512"]},"file":[{"file_name":"2024_StemCellResearch_Presen.pdf","file_id":"18641","relation":"main_file","checksum":"91edba8edde30d781dce89fdd5cadc39","success":1,"creator":"dernst","date_updated":"2024-12-10T08:28:17Z","date_created":"2024-12-10T08:28:17Z","file_size":6690494,"content_type":"application/pdf","access_level":"open_access"}],"isi":1,"publication":"Stem Cell Research and Therapy","publication_status":"published","acknowledgement":"We thank the personnel of the Lorenz-Böhler-Unfallkrankenhaus for providing the human tissue waste for primary cell isolation and the New York Stem Cell Foundation Research Institute for providing the human induced pluripotent stem cell line 1013 A and its mesenchymal progenitors. We also thank all our colleagues at the Ludwig Boltzmann Institute for Traumatology for their suggestions and ongoing support of the project. InstaText writing tool (https://instatext.io) was used to edit the English language of the final manuscript.\r\nThis work has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie actions (grant agreement No. 657716) and the Transforming European Industry call H2020-NMBP-TRIND-2020 (grant agreement No. 953134), as well as by the FFG Industrienahe Dissertation program (grant agreement No. 867803 and 853056), the FEMtech Praktika program (grant agreement No. 852154, 868917 and 877951) and the Production of the Future program (grant agreement No. 877452).","abstract":[{"lang":"eng","text":"Background: Human induced pluripotent stem cells represent a scalable source of youthful tissue progenitors and secretomes for regenerative therapies. The aim of our study was to investigate the potential of conditioned medium (CM) from hiPSC-mesenchymal progenitors (hiPSC-MPs) to stimulate osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells (MSCs). We also investigated whether prolonged cultivation or osteogenic pre-differentiation of hiPSC-MPs could enhance the stimulatory activity of CM.\r\nMethods: MSCs were isolated from 13 donors (age 20–90 years). CM derived from hiPSC-MPs was added to the MSC cultures and the effects on proliferation and osteogenic differentiation were examined after 14 days and 6 weeks. The stimulatory activity of hiPSC-MP-CM was compared with the activity of MSC-derived CM and with the activity of CM prepared from hiPSC-MPs pre-cultured in growth or osteogenic medium for 14 days. Comparative proteomic analysis of CM was performed to gain insight into the molecular components responsible for the stimulatory activity.\r\nResults: Primary bone marrow-derived MSC exhibited variability, with a tendency towards lower proliferation and tri-lineage differentiation in older donors. hiPSC-MP-CM increased the proliferation and alkaline phosphatase activity of MSC from several adult/aged donors after 14 days of continuous supplementation under osteogenic conditions. However, CM supplementation failed to improve the mineralization of MSC pellets after 6 weeks under osteogenic conditions. hiPSC-MP-CM showed greater enhancement of proliferation and ALP activity than CM derived from bone marrow-derived MSCs. Moreover, 14-day cultivation but not osteogenic pre-differentiation of hiPSC-MPs strongly enhanced CM stimulatory activity. Quantitative proteomic analysis of d14-CM revealed a distinct profile of components that formed a highly interconnected associations network with two clusters, one functionally associated with binding and organization of actin/cytoskeletal components and the other with structural constituents of the extracellular matrix, collagen, and growth factor binding. Several hub proteins were identified that were reported to have functions in cell-extracellular matrix interaction, osteogenic differentiation and development.\r\nConclusions: Our data show that hiPSC-MP-CM enhances early osteogenic differentiation of human bone marrow-derived MSCs and that prolonged cultivation of hiPSC-MPs enhances CM-stimulatory activity. Proteomic analysis of the upregulated protein components provides the basis for further optimization of hiPSC-MP-CM for bone regenerative therapies."}],"title":"Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium","author":[{"last_name":"Marolt Presen","first_name":"Darja","full_name":"Marolt Presen, Darja"},{"full_name":"Goeschl, Vanessa","first_name":"Vanessa","last_name":"Goeschl"},{"last_name":"Hanetseder","first_name":"Dominik","full_name":"Hanetseder, Dominik"},{"last_name":"Ogrin","first_name":"Laura","full_name":"Ogrin, Laura"},{"last_name":"Stetco","first_name":"Alexandra Larissa","full_name":"Stetco, Alexandra Larissa"},{"full_name":"Tansek, Anja","first_name":"Anja","last_name":"Tansek"},{"last_name":"Pozenel","full_name":"Pozenel, Laura","first_name":"Laura"},{"first_name":"Bella","full_name":"Bruszel, Bella","id":"70abbbb3-88ea-11ec-8e0a-e8c939944834","last_name":"Bruszel"},{"full_name":"Mitulovic, Goran","first_name":"Goran","last_name":"Mitulovic"},{"last_name":"Oesterreicher","full_name":"Oesterreicher, Johannes","first_name":"Johannes"},{"full_name":"Zipperle, Johannes","first_name":"Johannes","last_name":"Zipperle"},{"full_name":"Schaedl, Barbara","first_name":"Barbara","last_name":"Schaedl"},{"full_name":"Holnthoner, Wolfgang","first_name":"Wolfgang","last_name":"Holnthoner"},{"last_name":"Grillari","first_name":"Johannes","full_name":"Grillari, Johannes"},{"full_name":"Redl, Heinz","first_name":"Heinz","last_name":"Redl"}],"day":"01","ddc":["570"],"oa":1,"OA_place":"publisher","OA_type":"gold","article_processing_charge":"Yes"},{"publication":"Advanced Energy Materials","publication_identifier":{"eissn":["1614-6840"],"issn":["1614-6832"]},"file":[{"creator":"dernst","success":1,"file_size":8824301,"date_updated":"2024-07-22T12:07:56Z","date_created":"2024-07-22T12:07:56Z","content_type":"application/pdf","access_level":"open_access","file_name":"2024_AdvancedEnergyMaterials_Kleinhanns.pdf","file_id":"17314","relation":"main_file","checksum":"86b26430e00d5f43ea19e9b610692ab7"}],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"NanoFab"}],"isi":1,"external_id":{"isi":["001184300200001"]},"language":[{"iso":"eng"}],"year":"2024","article_type":"original","oa":1,"article_processing_charge":"Yes (via OA deal)","issue":"22","author":[{"id":"8BD9DE16-AB3C-11E9-9C8C-2A03E6697425","last_name":"Kleinhanns","orcid":"0000-0003-1537-7436","first_name":"Tobias","full_name":"Kleinhanns, Tobias"},{"full_name":"Milillo, Francesco","first_name":"Francesco","last_name":"Milillo","id":"38b830db-ea88-11ee-bf9b-929beaf79054"},{"orcid":"0000-0003-4566-5877","id":"45D7531A-F248-11E8-B48F-1D18A9856A87","last_name":"Calcabrini","first_name":"Mariano","full_name":"Calcabrini, Mariano"},{"id":"bd3fceba-dc74-11ea-a0a7-c17f71817366","last_name":"Fiedler","first_name":"Christine","full_name":"Fiedler, Christine"},{"last_name":"Horta","id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc","first_name":"Sharona","full_name":"Horta, Sharona"},{"orcid":"0000-0001-7597-043X","last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","first_name":"Daniel","full_name":"Balazs, Daniel"},{"last_name":"Strumolo","first_name":"Marissa J.","full_name":"Strumolo, Marissa J."},{"full_name":"Hasler, Roger","first_name":"Roger","last_name":"Hasler"},{"last_name":"Llorca","first_name":"Jordi","full_name":"Llorca, Jordi"},{"first_name":"Michael","full_name":"Tkadletz, Michael","last_name":"Tkadletz"},{"last_name":"Brutchey","first_name":"Richard L.","full_name":"Brutchey, Richard L."},{"id":"43C61214-F248-11E8-B48F-1D18A9856A87","last_name":"Ibáñez","orcid":"0000-0001-5013-2843","full_name":"Ibáñez, Maria","first_name":"Maria"}],"day":"12","ddc":["530"],"abstract":[{"text":"Thermoelectric materials convert heat into electricity, with a broad range of applications near room temperature (RT). However, the library of RT high-performance materials is limited. Traditional high-temperature synthetic methods constrain the range of materials achievable, hindering the ability to surpass crystal structure limitations and engineer defects. Here, a solution-based synthetic approach is introduced, enabling RT synthesis of powders and exploration of densification at lower temperatures to influence the material's microstructure. The approach is exemplified by Ag2Se, an n-type alternative to bismuth telluride. It is demonstrated that the concentration of Ag interstitials, grain boundaries, and dislocations are directly correlated to the sintering temperature, and achieve a figure of merit of 1.1 from RT to 100 °C after optimization. Moreover, insights into and resolve Ag2Se's challenges are provided, including stoichiometry issues leading to irreproducible performances. This work highlights the potential of RT solution synthesis in expanding the repertoire of high-performance thermoelectric materials for practical applications.","lang":"eng"}],"title":"A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se","publication_status":"published","acknowledgement":"This work was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Electron Microscopy Facility (EMF), the Lab Support Facility (LSF), and the Nanofabrication Facility (NNF). This work was financially supported by ISTA and the Werner Siemens Foundation. The USTEM Service Unit of the Technical University of Vienna is acknowledged for EBSD sample preparation and analysis. R.L.B. acknowledges the National Science Foundation for funding the mass spectrometry analysis under award DMR 1904719. J.L. is a Serra Húnter Fellow and is grateful to the ICREA Academia program and projects MICINN/FEDER PID2021-124572OB-C31 and GC 2021 SGR 01061.","file_date_updated":"2024-07-22T12:07:56Z","department":[{"_id":"MaIb"},{"_id":"LifeSc"}],"publisher":"Wiley","citation":{"ieee":"T. Kleinhanns <i>et al.</i>, “A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se,” <i>Advanced Energy Materials</i>, vol. 14, no. 22. Wiley, 2024.","apa":"Kleinhanns, T., Milillo, F., Calcabrini, M., Fiedler, C., Horta, S., Balazs, D., … Ibáñez, M. (2024). A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se. <i>Advanced Energy Materials</i>. Wiley. <a href=\"https://doi.org/10.1002/aenm.202400408\">https://doi.org/10.1002/aenm.202400408</a>","ama":"Kleinhanns T, Milillo F, Calcabrini M, et al. A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se. <i>Advanced Energy Materials</i>. 2024;14(22). doi:<a href=\"https://doi.org/10.1002/aenm.202400408\">10.1002/aenm.202400408</a>","chicago":"Kleinhanns, Tobias, Francesco Milillo, Mariano Calcabrini, Christine Fiedler, Sharona Horta, Daniel Balazs, Marissa J. Strumolo, et al. “A Route to High Thermoelectric Performance: Solution‐based Control of Microstructure and Composition in Ag2Se.” <i>Advanced Energy Materials</i>. Wiley, 2024. <a href=\"https://doi.org/10.1002/aenm.202400408\">https://doi.org/10.1002/aenm.202400408</a>.","short":"T. Kleinhanns, F. Milillo, M. Calcabrini, C. Fiedler, S. Horta, D. Balazs, M.J. Strumolo, R. Hasler, J. Llorca, M. Tkadletz, R.L. Brutchey, M. Ibáñez, Advanced Energy Materials 14 (2024).","mla":"Kleinhanns, Tobias, et al. “A Route to High Thermoelectric Performance: Solution‐based Control of Microstructure and Composition in Ag2Se.” <i>Advanced Energy Materials</i>, vol. 14, no. 22, 2400408, Wiley, 2024, doi:<a href=\"https://doi.org/10.1002/aenm.202400408\">10.1002/aenm.202400408</a>.","ista":"Kleinhanns T, Milillo F, Calcabrini M, Fiedler C, Horta S, Balazs D, Strumolo MJ, Hasler R, Llorca J, Tkadletz M, Brutchey RL, Ibáñez M. 2024. A route to high thermoelectric performance: Solution‐based control of microstructure and composition in Ag2Se. Advanced Energy Materials. 14(22), 2400408."},"date_published":"2024-06-12T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","intvolume":"        14","status":"public","corr_author":"1","article_number":"2400408","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"has_accepted_license":"1","type":"journal_article","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"22017"}]},"month":"06","_id":"15182","oa_version":"Published Version","volume":14,"date_updated":"2026-07-17T07:09:41Z","quality_controlled":"1","date_created":"2024-03-25T08:57:40Z","scopus_import":"1","project":[{"name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery","_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A"}],"doi":"10.1002/aenm.202400408"},{"type":"journal_article","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/how-sneaky-germs-hide-from-ants/"}]},"scopus_import":"1","project":[{"grant_number":"771402","call_identifier":"H2020","name":"Epidemics in ant societies on a chip","_id":"2649B4DE-B435-11E9-9278-68D0E5697425"},{"_id":"25DAF0B2-B435-11E9-9278-68D0E5697425","name":"Host-Parasite Coevolution","grant_number":"CR-118/3-1"}],"month":"03","_id":"12543","oa_version":"Published Version","date_updated":"2025-04-14T07:47:53Z","volume":7,"quality_controlled":"1","date_created":"2023-02-12T23:00:59Z","doi":"10.1038/s41559-023-01981-6","file_date_updated":"2023-08-16T11:54:59Z","department":[{"_id":"SyCr"},{"_id":"LifeSc"},{"_id":"JiFr"}],"publisher":"Springer Nature","intvolume":"         7","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Stock, Miriam, Barbara Milutinovic, Michaela Hönigsberger, Anna V Grasse, Florian Wiesenhofer, Niklas Kampleitner, Madhumitha Narasimhan, Thomas Schmitt, and Sylvia Cremer. “Pathogen Evasion of Social Immunity.” <i>Nature Ecology and Evolution</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41559-023-01981-6\">https://doi.org/10.1038/s41559-023-01981-6</a>.","short":"M. Stock, B. Milutinovic, M. Hönigsberger, A.V. Grasse, F. Wiesenhofer, N. Kampleitner, M. Narasimhan, T. Schmitt, S. Cremer, Nature Ecology and Evolution 7 (2023) 450–460.","mla":"Stock, Miriam, et al. “Pathogen Evasion of Social Immunity.” <i>Nature Ecology and Evolution</i>, vol. 7, Springer Nature, 2023, pp. 450–60, doi:<a href=\"https://doi.org/10.1038/s41559-023-01981-6\">10.1038/s41559-023-01981-6</a>.","ista":"Stock M, Milutinovic B, Hönigsberger M, Grasse AV, Wiesenhofer F, Kampleitner N, Narasimhan M, Schmitt T, Cremer S. 2023. Pathogen evasion of social immunity. Nature Ecology and Evolution. 7, 450–460.","ieee":"M. Stock <i>et al.</i>, “Pathogen evasion of social immunity,” <i>Nature Ecology and Evolution</i>, vol. 7. Springer Nature, pp. 450–460, 2023.","apa":"Stock, M., Milutinovic, B., Hönigsberger, M., Grasse, A. V., Wiesenhofer, F., Kampleitner, N., … Cremer, S. (2023). Pathogen evasion of social immunity. <i>Nature Ecology and Evolution</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41559-023-01981-6\">https://doi.org/10.1038/s41559-023-01981-6</a>","ama":"Stock M, Milutinovic B, Hönigsberger M, et al. Pathogen evasion of social immunity. <i>Nature Ecology and Evolution</i>. 2023;7:450-460. doi:<a href=\"https://doi.org/10.1038/s41559-023-01981-6\">10.1038/s41559-023-01981-6</a>"},"date_published":"2023-03-01T00:00:00Z","status":"public","corr_author":"1","ec_funded":1,"article_processing_charge":"No","oa":1,"author":[{"full_name":"Stock, Miriam","first_name":"Miriam","last_name":"Stock","id":"42462816-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-8214-4758","id":"2CDC32B8-F248-11E8-B48F-1D18A9856A87","last_name":"Milutinovic","first_name":"Barbara","full_name":"Milutinovic, Barbara"},{"id":"953894f3-25bd-11ec-8556-f70a9d38ef60","last_name":"Hönigsberger","full_name":"Hönigsberger, Michaela","first_name":"Michaela"},{"last_name":"Grasse","id":"406F989C-F248-11E8-B48F-1D18A9856A87","first_name":"Anna V","full_name":"Grasse, Anna V"},{"full_name":"Wiesenhofer, Florian","first_name":"Florian","last_name":"Wiesenhofer","id":"39523C54-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Niklas","full_name":"Kampleitner, Niklas","id":"2AC57FAC-F248-11E8-B48F-1D18A9856A87","last_name":"Kampleitner"},{"orcid":"0000-0002-8600-0671","last_name":"Narasimhan","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","first_name":"Madhumitha","full_name":"Narasimhan, Madhumitha"},{"last_name":"Schmitt","first_name":"Thomas","full_name":"Schmitt, Thomas"},{"full_name":"Cremer, Sylvia","first_name":"Sylvia","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","last_name":"Cremer","orcid":"0000-0002-2193-3868"}],"ddc":["570"],"day":"01","title":"Pathogen evasion of social immunity","abstract":[{"lang":"eng","text":"Treating sick group members is a hallmark of collective disease defence in vertebrates and invertebrates alike. Despite substantial effects on pathogen fitness and epidemiology, it is still largely unknown how pathogens react to the selection pressure imposed by care intervention. Using social insects and pathogenic fungi, we here performed a serial passage experiment in the presence or absence of colony members, which provide social immunity by grooming off infectious spores from exposed individuals. We found specific effects on pathogen diversity, virulence and transmission. Under selection of social immunity, pathogens invested into higher spore production, but spores were less virulent. Notably, they also elicited a lower grooming response in colony members, compared with spores from the individual host selection lines. Chemical spore analysis suggested that the spores from social selection lines escaped the caregivers’ detection by containing lower levels of ergosterol, a key fungal membrane component. Experimental application of chemically pure ergosterol indeed induced sanitary grooming, supporting its role as a microbe-associated cue triggering host social immunity against fungal pathogens. By reducing this detection cue, pathogens were able to evade the otherwise very effective collective disease defences of their social hosts."}],"acknowledgement":"We thank B. M. Steinwender, N. V. Meyling and J. Eilenberg for the fungal strains; J. Anaya-Rojas for statistical advice; the Social Immunity team at ISTA for ant collection and experimental help, in particular H. Leitner, and the ISTA Lab Support Facility for general laboratory support; D. Ebert, H. Schulenburg and J. Heinze for continued project discussion; and M. Sixt, R. Roemhild and the Social Immunity team for comments on the manuscript. The study was funded by the German Research Foundation (CR118/3-1) within the Framework of the Priority Program SPP 1399, and the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (No. 771402; EPIDEMICSonCHIP), both to S.C.","publication_status":"published","page":"450-460","publication":"Nature Ecology and Evolution","file":[{"file_size":1600499,"date_created":"2023-08-16T11:54:59Z","date_updated":"2023-08-16T11:54:59Z","success":1,"creator":"dernst","content_type":"application/pdf","access_level":"open_access","file_id":"14069","relation":"main_file","file_name":"2023_NatureEcoEvo_Stock.pdf","checksum":"8244f4650a0e7aeea488d1bcd4a31702"}],"isi":1,"acknowledged_ssus":[{"_id":"LifeSc"}],"publication_identifier":{"eissn":["2397-334X"]},"pmid":1,"external_id":{"isi":["000924572800001"],"pmid":["36732670"]},"language":[{"iso":"eng"}],"year":"2023","article_type":"original"},{"department":[{"_id":"LifeSc"}],"publisher":"Springer Nature","intvolume":"        30","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2023-05-01T00:00:00Z","citation":{"ista":"Filipović Marijić V, Krasnici N, Valić D, Kapetanović D, Vardić Smrzlić I, Jordanova M, Rebok K, Ramani S, Kostov V, Nastova R, Dragun Z. 2023. Pollution impact on metal and biomarker responses in intestinal cytosol of freshwater fish. Environmental Science and Pollution Research. 30, 63510–63521.","mla":"Filipović Marijić, Vlatka, et al. “Pollution Impact on Metal and Biomarker Responses in Intestinal Cytosol of Freshwater Fish.” <i>Environmental Science and Pollution Research</i>, vol. 30, Springer Nature, 2023, pp. 63510–21, doi:<a href=\"https://doi.org/10.1007/s11356-023-26844-2\">10.1007/s11356-023-26844-2</a>.","short":"V. Filipović Marijić, N. Krasnici, D. Valić, D. Kapetanović, I. Vardić Smrzlić, M. Jordanova, K. Rebok, S. Ramani, V. Kostov, R. Nastova, Z. Dragun, Environmental Science and Pollution Research 30 (2023) 63510–63521.","chicago":"Filipović Marijić, Vlatka, Nesrete Krasnici, Damir Valić, Damir Kapetanović, Irena Vardić Smrzlić, Maja Jordanova, Katerina Rebok, et al. “Pollution Impact on Metal and Biomarker Responses in Intestinal Cytosol of Freshwater Fish.” <i>Environmental Science and Pollution Research</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1007/s11356-023-26844-2\">https://doi.org/10.1007/s11356-023-26844-2</a>.","ama":"Filipović Marijić V, Krasnici N, Valić D, et al. Pollution impact on metal and biomarker responses in intestinal cytosol of freshwater fish. <i>Environmental Science and Pollution Research</i>. 2023;30:63510-63521. doi:<a href=\"https://doi.org/10.1007/s11356-023-26844-2\">10.1007/s11356-023-26844-2</a>","apa":"Filipović Marijić, V., Krasnici, N., Valić, D., Kapetanović, D., Vardić Smrzlić, I., Jordanova, M., … Dragun, Z. (2023). Pollution impact on metal and biomarker responses in intestinal cytosol of freshwater fish. <i>Environmental Science and Pollution Research</i>. Springer Nature. <a href=\"https://doi.org/10.1007/s11356-023-26844-2\">https://doi.org/10.1007/s11356-023-26844-2</a>","ieee":"V. Filipović Marijić <i>et al.</i>, “Pollution impact on metal and biomarker responses in intestinal cytosol of freshwater fish,” <i>Environmental Science and Pollution Research</i>, vol. 30. Springer Nature, pp. 63510–63521, 2023."},"status":"public","type":"journal_article","scopus_import":"1","date_created":"2023-04-23T22:01:03Z","volume":30,"date_updated":"2023-10-04T11:23:10Z","quality_controlled":"1","_id":"12863","oa_version":"None","month":"05","doi":"10.1007/s11356-023-26844-2","publication":"Environmental Science and Pollution Research","isi":1,"publication_identifier":{"issn":["0944-1344"],"eissn":["1614-7499"]},"language":[{"iso":"eng"}],"external_id":{"isi":["000970917900012"],"pmid":["37055686"]},"pmid":1,"article_type":"original","year":"2023","article_processing_charge":"No","day":"01","author":[{"full_name":"Filipović Marijić, Vlatka","first_name":"Vlatka","last_name":"Filipović Marijić"},{"first_name":"Nesrete","full_name":"Krasnici, Nesrete","id":"cb5852d4-287f-11ed-baf0-bc1dd2d5c745","last_name":"Krasnici"},{"last_name":"Valić","first_name":"Damir","full_name":"Valić, Damir"},{"last_name":"Kapetanović","first_name":"Damir","full_name":"Kapetanović, Damir"},{"first_name":"Irena","full_name":"Vardić Smrzlić, Irena","last_name":"Vardić Smrzlić"},{"full_name":"Jordanova, Maja","first_name":"Maja","last_name":"Jordanova"},{"full_name":"Rebok, Katerina","first_name":"Katerina","last_name":"Rebok"},{"last_name":"Ramani","first_name":"Sheriban","full_name":"Ramani, Sheriban"},{"full_name":"Kostov, Vasil","first_name":"Vasil","last_name":"Kostov"},{"first_name":"Rodne","full_name":"Nastova, Rodne","last_name":"Nastova"},{"first_name":"Zrinka","full_name":"Dragun, Zrinka","last_name":"Dragun"}],"title":"Pollution impact on metal and biomarker responses in intestinal cytosol of freshwater fish","abstract":[{"lang":"eng","text":"In the present study, essential and nonessential metal content and biomarker responses were investigated in the intestine of fish collected from the areas polluted by mining. Our objective was to determine metal and biomarker levels in tissue responsible for dietary intake, which is rarely studied in water pollution research. The study was conducted in the Bregalnica River, reference location, and in the Zletovska and Kriva Rivers (the Republic of North Macedonia), which are directly influenced by the active mines Zletovo and Toranica, respectively. Biological responses were analyzed in Vardar chub (Squalius vardarensis; Karaman, 1928), using for the first time intestinal cytosol as a potentially toxic cell fraction, since metal sensitivity is mostly associated with cytosol. Cytosolic metal levels were higher in fish under the influence of mining (Tl, Li, Cs, Mo, Sr, Cd, Rb, and Cu in the Zletovska River and Cr, Pb, and Se in the Kriva River compared to the Bregalnica River in both seasons). The same trend was evident for total proteins, biomarkers of general stress, and metallothioneins, biomarkers of metal exposure, indicating cellular disturbances in the intestine, the primary site of dietary metal uptake. The association of cytosolic Cu and Cd at all locations pointed to similar pathways and homeostasis of these metallothionein-binding metals. Comparison with other indicator tissues showed that metal concentrations were higher in the intestine of fish from mining-affected areas than in the liver and gills. In general, these results indicated the importance of dietary metal pathways, and cytosolic metal fraction in assessing pollution impacts in freshwater ecosystems."}],"acknowledgement":"The authors are grateful to Dr. Nevenka Mikac for the opportunity to perform metal measurements on HR ICP-MS. This research was funded by the Ministry of Science, Education and Sport of the Republic of Croatia (projects No. 098–0982934-2721 and 098–1782739-2749). The sampling was carried out as a part of two Croatian-Macedonian bilateral projects: “The assessment of availability and effects of metals on fish in the rivers under the impact of mining activities” and “Bacterial and parasitical communities of chub as indicators of the status of environment exposed to mining activities.”","page":"63510-63521","publication_status":"published"},{"publication":"Science","isi":1,"publication_identifier":{"eissn":["1095-9203"]},"language":[{"iso":"eng"}],"external_id":{"isi":["001100656800023"],"pmid":["37769110"]},"pmid":1,"article_type":"letter_note","year":"2023","article_processing_charge":"No","day":"29","author":[{"full_name":"Balazs, Daniel","first_name":"Daniel","last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","orcid":"0000-0001-7597-043X"},{"full_name":"Ibáñez, Maria","first_name":"Maria","last_name":"Ibáñez","id":"43C61214-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5013-2843"}],"issue":"6665","title":"Widening the use of 3D printing","abstract":[{"text":"A light-triggered fabrication method extends the functionality of printable nanomaterials","lang":"eng"}],"acknowledgement":"The authors thank the Werner-Siemens-Stiftung and the Institute of Science and Technology Austria for financial support.","page":"1413-1414","publication_status":"published","department":[{"_id":"MaIb"},{"_id":"LifeSc"}],"publisher":"AAAS","intvolume":"       381","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"ieee":"D. Balazs and M. Ibáñez, “Widening the use of 3D printing,” <i>Science</i>, vol. 381, no. 6665. AAAS, pp. 1413–1414, 2023.","apa":"Balazs, D., &#38; Ibáñez, M. (2023). Widening the use of 3D printing. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adk3070\">https://doi.org/10.1126/science.adk3070</a>","ama":"Balazs D, Ibáñez M. Widening the use of 3D printing. <i>Science</i>. 2023;381(6665):1413-1414. doi:<a href=\"https://doi.org/10.1126/science.adk3070\">10.1126/science.adk3070</a>","chicago":"Balazs, Daniel, and Maria Ibáñez. “Widening the Use of 3D Printing.” <i>Science</i>. AAAS, 2023. <a href=\"https://doi.org/10.1126/science.adk3070\">https://doi.org/10.1126/science.adk3070</a>.","short":"D. Balazs, M. Ibáñez, Science 381 (2023) 1413–1414.","mla":"Balazs, Daniel, and Maria Ibáñez. “Widening the Use of 3D Printing.” <i>Science</i>, vol. 381, no. 6665, AAAS, 2023, pp. 1413–14, doi:<a href=\"https://doi.org/10.1126/science.adk3070\">10.1126/science.adk3070</a>.","ista":"Balazs D, Ibáñez M. 2023. Widening the use of 3D printing. Science. 381(6665), 1413–1414."},"date_published":"2023-09-29T00:00:00Z","corr_author":"1","status":"public","type":"journal_article","project":[{"_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A","name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery"}],"scopus_import":"1","volume":381,"date_updated":"2025-09-09T13:04:47Z","date_created":"2023-10-08T22:01:16Z","quality_controlled":"1","oa_version":"None","_id":"14404","month":"09","doi":"10.1126/science.adk3070"},{"day":"20","author":[{"last_name":"Filipović Marijić","first_name":"Vlatka","full_name":"Filipović Marijić, Vlatka"},{"full_name":"Subirana, Maria Angels","first_name":"Maria Angels","last_name":"Subirana"},{"first_name":"Dirk","full_name":"Schaumlöffel, Dirk","last_name":"Schaumlöffel"},{"first_name":"Josip","full_name":"Barišić, Josip","last_name":"Barišić"},{"full_name":"Gontier, Etienne","first_name":"Etienne","last_name":"Gontier"},{"first_name":"Nesrete","full_name":"Krasnici, Nesrete","last_name":"Krasnici","id":"cb5852d4-287f-11ed-baf0-bc1dd2d5c745"},{"first_name":"Tatjana","full_name":"Mijošek, Tatjana","last_name":"Mijošek"},{"last_name":"Hernández-Orts","first_name":"Jesús S.","full_name":"Hernández-Orts, Jesús S."},{"first_name":"Tomáš","full_name":"Scholz, Tomáš","last_name":"Scholz"},{"full_name":"Erk, Marijana","first_name":"Marijana","last_name":"Erk"}],"oa":1,"OA_place":"repository","article_processing_charge":"No","OA_type":"green","publication_status":"published","acknowledgement":"The authors thank the Czech Science Foundation (project No. 19-28399X) and the Czech Academy of Sciences (RVO: 60077344) and are sincerely grateful to the Bordeaux Imaging Centre (member of the France BioImaging national infrastructure, ANR-10-INBS-04) for help with TEM and to members of the Laboratory of Biological Effects of Metals and Laboratory of Aquaculture and Pathology of Aquatic Organisms (Ruđer Bošković Institute, Croatia) for the assistance with fieldwork.","abstract":[{"lang":"eng","text":"Acanthocephalans, intestinal parasites of vertebrates, are characterised by orders of magnitude higher metal accumulation than free-living organisms, but the mechanism of such effective metal accumulation is still unknown. The aim of our study was to gain new insights into the high-resolution localization of elements in the bodies of acanthocephalans, thus taking an initial step towards elucidating metal uptake and accumulation in organisms under real environmental conditions. For the first time, nanoscale secondary ion mass spectrometry (NanoSIMS) was used for high-resolution mapping of 12 elements (C, Ca, Cu, Fe, N, Na, O, P, Pb, S, Se, and Tl) in three selected body parts (trunk spines, inner part of the proboscis receptacle and inner surface of the tegument) of Dentitruncus truttae, a parasite of brown trout (Salmo trutta) from the Krka River in Croatia. In addition, the same body parts were examined using transmission electron microscopy (TEM) and correlated with NanoSIMS images. Metal concentrations determined using HR ICP-MS confirmed higher accumulation in D. truttae than in the fish intestine. The chemical composition of the acanthocephalan body showed the highest density of C, Ca, N, Na, O, S, as important and constitutive elements in living cells in all studied structures, while Fe was predominant among trace elements. In general, higher element density was found in trunk spines and tegument, as body structures responsible for substance absorption in parasites. The results obtained with NanoSIMS and TEM-NanoSIMS correlative imaging represent pilot data for mapping of elements at nanoscale resolution in the ultrastructure of various body parts of acanthocephalans and generally provide a contribution for further application of this technique in all parasite species."}],"title":"First insight in element localisation in different body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS","publication_identifier":{"issn":["0048-9697"]},"isi":1,"publication":"Science of The Total Environment","article_type":"original","year":"2023","language":[{"iso":"eng"}],"pmid":1,"external_id":{"isi":["001002645100001"],"pmid":["37169189"]},"keyword":["Pollution","Waste Management and Disposal","Environmental Chemistry","Environmental Engineering"],"type":"journal_article","main_file_link":[{"url":"https://hal.science/hal-04296657/document","open_access":"1"}],"doi":"10.1016/j.scitotenv.2023.164010","volume":887,"date_created":"2024-01-10T10:43:08Z","quality_controlled":"1","date_updated":"2025-04-24T11:35:33Z","month":"08","_id":"14786","oa_version":"Submitted Version","scopus_import":"1","publisher":"Elsevier","department":[{"_id":"LifeSc"}],"status":"public","article_number":"164010","date_published":"2023-08-20T00:00:00Z","citation":{"ieee":"V. Filipović Marijić <i>et al.</i>, “First insight in element localisation in different body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS,” <i>Science of The Total Environment</i>, vol. 887. Elsevier, 2023.","apa":"Filipović Marijić, V., Subirana, M. A., Schaumlöffel, D., Barišić, J., Gontier, E., Krasnici, N., … Erk, M. (2023). First insight in element localisation in different body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS. <i>Science of The Total Environment</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.scitotenv.2023.164010\">https://doi.org/10.1016/j.scitotenv.2023.164010</a>","ama":"Filipović Marijić V, Subirana MA, Schaumlöffel D, et al. First insight in element localisation in different body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS. <i>Science of The Total Environment</i>. 2023;887. doi:<a href=\"https://doi.org/10.1016/j.scitotenv.2023.164010\">10.1016/j.scitotenv.2023.164010</a>","chicago":"Filipović Marijić, Vlatka, Maria Angels Subirana, Dirk Schaumlöffel, Josip Barišić, Etienne Gontier, Nesrete Krasnici, Tatjana Mijošek, Jesús S. Hernández-Orts, Tomáš Scholz, and Marijana Erk. “First Insight in Element Localisation in Different Body Parts of the Acanthocephalan Dentitruncus Truttae Using TEM and NanoSIMS.” <i>Science of The Total Environment</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.scitotenv.2023.164010\">https://doi.org/10.1016/j.scitotenv.2023.164010</a>.","short":"V. Filipović Marijić, M.A. Subirana, D. Schaumlöffel, J. Barišić, E. Gontier, N. Krasnici, T. Mijošek, J.S. Hernández-Orts, T. Scholz, M. Erk, Science of The Total Environment 887 (2023).","mla":"Filipović Marijić, Vlatka, et al. “First Insight in Element Localisation in Different Body Parts of the Acanthocephalan Dentitruncus Truttae Using TEM and NanoSIMS.” <i>Science of The Total Environment</i>, vol. 887, 164010, Elsevier, 2023, doi:<a href=\"https://doi.org/10.1016/j.scitotenv.2023.164010\">10.1016/j.scitotenv.2023.164010</a>.","ista":"Filipović Marijić V, Subirana MA, Schaumlöffel D, Barišić J, Gontier E, Krasnici N, Mijošek T, Hernández-Orts JS, Scholz T, Erk M. 2023. First insight in element localisation in different body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS. Science of The Total Environment. 887, 164010."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"       887"},{"file_date_updated":"2024-01-17T07:47:35Z","department":[{"_id":"LifeSc"}],"publisher":"International Union of Crystallography","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"        56","date_published":"2023-12-01T00:00:00Z","citation":{"ama":"Pauw BR, Smales GJ, Anker AS, et al. The human factor: Results of a small-angle scattering data analysis round robin. <i>Journal of Applied Crystallography</i>. 2023;56(6):1618-1629. doi:<a href=\"https://doi.org/10.1107/S1600576723008324\">10.1107/S1600576723008324</a>","ieee":"B. R. Pauw <i>et al.</i>, “The human factor: Results of a small-angle scattering data analysis round robin,” <i>Journal of Applied Crystallography</i>, vol. 56, no. 6. International Union of Crystallography, pp. 1618–1629, 2023.","apa":"Pauw, B. R., Smales, G. J., Anker, A. S., Annadurai, V., Balazs, D., Bienert, R., … Wuttke, J. (2023). The human factor: Results of a small-angle scattering data analysis round robin. <i>Journal of Applied Crystallography</i>. International Union of Crystallography. <a href=\"https://doi.org/10.1107/S1600576723008324\">https://doi.org/10.1107/S1600576723008324</a>","ista":"Pauw BR, Smales GJ, Anker AS, Annadurai V, Balazs D, Bienert R, Bouwman WG, Breßler I, Breternitz J, Brok ES, Bryant G, Clulow AJ, Crater ER, De Geuser F, Giudice AD, Deumer J, Disch S, Dutt S, Frank K, Fratini E, Garcia PRAF, Gilbert EP, Hahn MB, Hallett J, Hohenschutz M, Hollamby M, Huband S, Ilavsky J, Jochum JK, Juelsholt M, Mansel BW, Penttilä P, Pittkowski RK, Portale G, Pozzo LD, Rochels L, Rosalie JM, Saloga PEJ, Seibt S, Smith AJ, Smith GN, Spiering GA, Stawski TM, Taché O, Thünemann AF, Toth K, Whitten AE, Wuttke J. 2023. The human factor: Results of a small-angle scattering data analysis round robin. Journal of Applied Crystallography. 56(6), 1618–1629.","chicago":"Pauw, Brian R., Glen J. Smales, Andy S. Anker, Venkatasamy Annadurai, Daniel Balazs, Ralf Bienert, Wim G. Bouwman, et al. “The Human Factor: Results of a Small-Angle Scattering Data Analysis Round Robin.” <i>Journal of Applied Crystallography</i>. International Union of Crystallography, 2023. <a href=\"https://doi.org/10.1107/S1600576723008324\">https://doi.org/10.1107/S1600576723008324</a>.","short":"B.R. Pauw, G.J. Smales, A.S. Anker, V. Annadurai, D. Balazs, R. Bienert, W.G. Bouwman, I. Breßler, J. Breternitz, E.S. Brok, G. Bryant, A.J. Clulow, E.R. Crater, F. De Geuser, A.D. Giudice, J. Deumer, S. Disch, S. Dutt, K. Frank, E. Fratini, P.R.A.F. Garcia, E.P. Gilbert, M.B. Hahn, J. Hallett, M. Hohenschutz, M. Hollamby, S. Huband, J. Ilavsky, J.K. Jochum, M. Juelsholt, B.W. Mansel, P. Penttilä, R.K. Pittkowski, G. Portale, L.D. Pozzo, L. Rochels, J.M. Rosalie, P.E.J. Saloga, S. Seibt, A.J. Smith, G.N. Smith, G.A. Spiering, T.M. Stawski, O. Taché, A.F. Thünemann, K. Toth, A.E. Whitten, J. Wuttke, Journal of Applied Crystallography 56 (2023) 1618–1629.","mla":"Pauw, Brian R., et al. “The Human Factor: Results of a Small-Angle Scattering Data Analysis Round Robin.” <i>Journal of Applied Crystallography</i>, vol. 56, no. 6, International Union of Crystallography, 2023, pp. 1618–29, doi:<a href=\"https://doi.org/10.1107/S1600576723008324\">10.1107/S1600576723008324</a>."},"arxiv":1,"status":"public","type":"journal_article","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","scopus_import":"1","_id":"14799","oa_version":"Published Version","month":"12","volume":56,"quality_controlled":"1","date_updated":"2026-07-07T06:18:55Z","date_created":"2024-01-14T23:00:57Z","doi":"10.1107/S1600576723008324","publication":"Journal of Applied Crystallography","file":[{"file_name":"2023_JourApplCrystallography_Pauw.pdf","relation":"main_file","file_id":"14822","checksum":"dab30d4556360f2cecf99f4b7efb0ee9","creator":"dernst","success":1,"date_created":"2024-01-17T07:47:35Z","date_updated":"2024-01-17T07:47:35Z","file_size":2165864,"access_level":"open_access","content_type":"application/pdf"}],"isi":1,"das_tickbox":"1","publication_identifier":{"eissn":["1600-5767"],"issn":["0021-8898"]},"external_id":{"arxiv":["2303.03772"],"isi":["001120776200002"]},"language":[{"iso":"eng"}],"year":"2023","article_type":"original","article_processing_charge":"Yes (via OA deal)","oa":1,"author":[{"first_name":"Brian R.","full_name":"Pauw, Brian R.","last_name":"Pauw"},{"full_name":"Smales, Glen J.","first_name":"Glen J.","last_name":"Smales"},{"first_name":"Andy S.","full_name":"Anker, Andy S.","last_name":"Anker"},{"first_name":"Venkatasamy","full_name":"Annadurai, Venkatasamy","last_name":"Annadurai"},{"last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","orcid":"0000-0001-7597-043X","first_name":"Daniel","full_name":"Balazs, Daniel"},{"last_name":"Bienert","first_name":"Ralf","full_name":"Bienert, Ralf"},{"last_name":"Bouwman","first_name":"Wim G.","full_name":"Bouwman, Wim G."},{"first_name":"Ingo","full_name":"Breßler, Ingo","last_name":"Breßler"},{"last_name":"Breternitz","full_name":"Breternitz, Joachim","first_name":"Joachim"},{"last_name":"Brok","first_name":"Erik S.","full_name":"Brok, Erik S."},{"first_name":"Gary","full_name":"Bryant, Gary","last_name":"Bryant"},{"first_name":"Andrew J.","full_name":"Clulow, Andrew J.","last_name":"Clulow"},{"full_name":"Crater, Erin R.","first_name":"Erin R.","last_name":"Crater"},{"full_name":"De Geuser, Frédéric","first_name":"Frédéric","last_name":"De Geuser"},{"first_name":"Alessandra Del","full_name":"Giudice, Alessandra Del","last_name":"Giudice"},{"first_name":"Jérôme","full_name":"Deumer, Jérôme","last_name":"Deumer"},{"first_name":"Sabrina","full_name":"Disch, Sabrina","last_name":"Disch"},{"full_name":"Dutt, Shankar","first_name":"Shankar","last_name":"Dutt"},{"first_name":"Kilian","full_name":"Frank, Kilian","last_name":"Frank"},{"last_name":"Fratini","full_name":"Fratini, Emiliano","first_name":"Emiliano"},{"last_name":"Garcia","full_name":"Garcia, Paulo R.A.F.","first_name":"Paulo R.A.F."},{"first_name":"Elliot P.","full_name":"Gilbert, Elliot P.","last_name":"Gilbert"},{"last_name":"Hahn","first_name":"Marc B.","full_name":"Hahn, Marc B."},{"first_name":"James","full_name":"Hallett, James","last_name":"Hallett"},{"last_name":"Hohenschutz","first_name":"Max","full_name":"Hohenschutz, Max"},{"first_name":"Martin","full_name":"Hollamby, Martin","last_name":"Hollamby"},{"first_name":"Steven","full_name":"Huband, Steven","last_name":"Huband"},{"first_name":"Jan","full_name":"Ilavsky, Jan","last_name":"Ilavsky"},{"full_name":"Jochum, Johanna K.","first_name":"Johanna K.","last_name":"Jochum"},{"last_name":"Juelsholt","first_name":"Mikkel","full_name":"Juelsholt, Mikkel"},{"last_name":"Mansel","first_name":"Bradley W.","full_name":"Mansel, Bradley W."},{"last_name":"Penttilä","full_name":"Penttilä, Paavo","first_name":"Paavo"},{"first_name":"Rebecca K.","full_name":"Pittkowski, Rebecca K.","last_name":"Pittkowski"},{"last_name":"Portale","first_name":"Giuseppe","full_name":"Portale, Giuseppe"},{"full_name":"Pozzo, Lilo D.","first_name":"Lilo D.","last_name":"Pozzo"},{"full_name":"Rochels, Leonhard","first_name":"Leonhard","last_name":"Rochels"},{"last_name":"Rosalie","first_name":"Julian M.","full_name":"Rosalie, Julian M."},{"last_name":"Saloga","first_name":"Patrick E.J.","full_name":"Saloga, Patrick E.J."},{"first_name":"Susanne","full_name":"Seibt, Susanne","last_name":"Seibt"},{"full_name":"Smith, Andrew J.","first_name":"Andrew J.","last_name":"Smith"},{"first_name":"Gregory N.","full_name":"Smith, Gregory N.","last_name":"Smith"},{"full_name":"Spiering, Glenn A.","first_name":"Glenn A.","last_name":"Spiering"},{"last_name":"Stawski","full_name":"Stawski, Tomasz M.","first_name":"Tomasz M."},{"first_name":"Olivier","full_name":"Taché, Olivier","last_name":"Taché"},{"full_name":"Thünemann, Andreas F.","first_name":"Andreas F.","last_name":"Thünemann"},{"last_name":"Toth","first_name":"Kristof","full_name":"Toth, Kristof"},{"last_name":"Whitten","first_name":"Andrew E.","full_name":"Whitten, Andrew E."},{"last_name":"Wuttke","first_name":"Joachim","full_name":"Wuttke, Joachim"}],"ddc":["540"],"day":"01","issue":"6","title":"The human factor: Results of a small-angle scattering data analysis round robin","abstract":[{"text":"A round-robin study has been carried out to estimate the impact of the human element in small-angle scattering data analysis. Four corrected datasets were provided to participants ready for analysis. All datasets were measured on samples containing spherical scatterers, with two datasets in dilute dispersions and two from powders. Most of the 46 participants correctly identified the number of populations in the dilute dispersions, with half of the population\r\nmean entries within 1.5% and half of the population width entries within 40%. Due to the added complexity of the structure factor, far fewer people submitted answers on the powder datasets. For those that did, half of the entries for the means and widths were within 44 and 86%, respectively. This round-robin experiment highlights several causes for the discrepancies, for which solutions are proposed.","lang":"eng"}],"acknowledgement":"KT acknowledges the NIST–NRC postdoctoral fellowship program for support. This work was partially funded through the European Metrology Programme for Innovation and Research (EMPIR) project No. 17NRM04.\r\nCertain commercial equipment, instruments, materials or software are identified in this article in order to specify the experimental procedure adequately. Such identification is not intended to imply recommendation or endorsement by NIST, nor is it intended to imply that the materials or equipment identified are necessarily the best available for the purpose. Open access funding enabled and organized by Projekt DEAL.","publication_status":"published","page":"1618-1629"},{"publication_identifier":{"eissn":["2753-149X"]},"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"PreCl"},{"_id":"Bio"}],"file":[{"relation":"main_file","file_id":"14061","file_name":"2023_OxfordOpenNeuroscience_Hansen.pdf","checksum":"822e76e056c07099d1fb27d1ece5941b","date_created":"2023-08-16T08:00:30Z","date_updated":"2023-08-16T08:00:30Z","file_size":4846551,"creator":"dernst","success":1,"access_level":"open_access","content_type":"application/pdf"}],"publication":"Oxford Open Neuroscience","article_type":"original","year":"2022","language":[{"iso":"eng"}],"external_id":{"pmid":["38596707"]},"pmid":1,"issue":"1","day":"07","ddc":["570"],"author":[{"id":"38853E16-F248-11E8-B48F-1D18A9856A87","last_name":"Hansen","full_name":"Hansen, Andi H","first_name":"Andi H"},{"first_name":"Florian","full_name":"Pauler, Florian","orcid":"0000-0002-7462-0048","last_name":"Pauler","id":"48EA0138-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Riedl","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4844-6311","full_name":"Riedl, Michael","first_name":"Michael"},{"last_name":"Streicher","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","full_name":"Streicher, Carmen","first_name":"Carmen"},{"full_name":"Heger, Anna-Magdalena","first_name":"Anna-Magdalena","last_name":"Heger","id":"4B76FFD2-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-7903-3010","last_name":"Laukoter","id":"2D6B7A9A-F248-11E8-B48F-1D18A9856A87","full_name":"Laukoter, Susanne","first_name":"Susanne"},{"last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M","first_name":"Christoph M"},{"id":"2A103192-F248-11E8-B48F-1D18A9856A87","last_name":"Nicolas","full_name":"Nicolas, Armel","first_name":"Armel"},{"last_name":"Hof","id":"3A374330-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2057-2754","first_name":"Björn","full_name":"Hof, Björn"},{"last_name":"Tsai","first_name":"Li Huei","full_name":"Tsai, Li Huei"},{"first_name":"Thomas","full_name":"Rülicke, Thomas","last_name":"Rülicke"},{"full_name":"Hippenmeyer, Simon","first_name":"Simon","orcid":"0000-0003-2279-1061","last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87"}],"oa":1,"article_processing_charge":"No","publication_status":"published","acknowledgement":"A.H.H. was a recipient of a DOC Fellowship (24812) of the Austrian Academy of Sciences. This work also received support from IST Austria institutional funds; the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007–2013) under REA grant agreement No 618444 to S.H.\r\nAPC funding was obtained by IST Austria institutional funds.\r\nWe thank A. Sommer and C. Czepe (VBCF GmbH, NGS Unit), L. Andersen, J. Sonntag and J. Renno for technical support and/or initial experiments; M. Sixt, J. Nimpf and all members of the Hippenmeyer lab for discussion. This research was supported by the Scientific Service Units of IST Austria through resources provided by the Imaging and Optics Facility, Lab Support Facility and Preclinical Facility.","abstract":[{"lang":"eng","text":"The mammalian neocortex is composed of diverse neuronal and glial cell classes that broadly arrange in six distinct laminae. Cortical layers emerge during development and defects in the developmental programs that orchestrate cortical lamination are associated with neurodevelopmental diseases. The developmental principle of cortical layer formation depends on concerted radial projection neuron migration, from their birthplace to their final target position. Radial migration occurs in defined sequential steps, regulated by a large array of signaling pathways. However, based on genetic loss-of-function experiments, most studies have thus far focused on the role of cell-autonomous gene function. Yet, cortical neuron migration in situ is a complex process and migrating neurons traverse along diverse cellular compartments and environments. The role of tissue-wide properties and genetic state in radial neuron migration is however not clear. Here we utilized mosaic analysis with double markers (MADM) technology to either sparsely or globally delete gene function, followed by quantitative single-cell phenotyping. The MADM-based gene ablation paradigms in combination with computational modeling demonstrated that global tissue-wide effects predominate cell-autonomous gene function albeit in a gene-specific manner. Our results thus suggest that the genetic landscape in a tissue critically affects the overall migration phenotype of individual cortical projection neurons. In a broader context, our findings imply that global tissue-wide effects represent an essential component of the underlying etiology associated with focal malformations of cortical development in particular, and neurological diseases in general."}],"title":"Tissue-wide effects override cell-intrinsic gene function in radial neuron migration","publisher":"Oxford University Press","department":[{"_id":"SiHi"},{"_id":"BjHo"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"file_date_updated":"2023-08-16T08:00:30Z","ec_funded":1,"corr_author":"1","status":"public","article_number":"kvac009","citation":{"ama":"Hansen AH, Pauler F, Riedl M, et al. Tissue-wide effects override cell-intrinsic gene function in radial neuron migration. <i>Oxford Open Neuroscience</i>. 2022;1(1). doi:<a href=\"https://doi.org/10.1093/oons/kvac009\">10.1093/oons/kvac009</a>","apa":"Hansen, A. H., Pauler, F., Riedl, M., Streicher, C., Heger, A.-M., Laukoter, S., … Hippenmeyer, S. (2022). Tissue-wide effects override cell-intrinsic gene function in radial neuron migration. <i>Oxford Open Neuroscience</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/oons/kvac009\">https://doi.org/10.1093/oons/kvac009</a>","ieee":"A. H. Hansen <i>et al.</i>, “Tissue-wide effects override cell-intrinsic gene function in radial neuron migration,” <i>Oxford Open Neuroscience</i>, vol. 1, no. 1. Oxford University Press, 2022.","ista":"Hansen AH, Pauler F, Riedl M, Streicher C, Heger A-M, Laukoter S, Sommer CM, Nicolas A, Hof B, Tsai LH, Rülicke T, Hippenmeyer S. 2022. Tissue-wide effects override cell-intrinsic gene function in radial neuron migration. Oxford Open Neuroscience. 1(1), kvac009.","mla":"Hansen, Andi H., et al. “Tissue-Wide Effects Override Cell-Intrinsic Gene Function in Radial Neuron Migration.” <i>Oxford Open Neuroscience</i>, vol. 1, no. 1, kvac009, Oxford University Press, 2022, doi:<a href=\"https://doi.org/10.1093/oons/kvac009\">10.1093/oons/kvac009</a>.","short":"A.H. Hansen, F. Pauler, M. Riedl, C. Streicher, A.-M. Heger, S. Laukoter, C.M. Sommer, A. Nicolas, B. Hof, L.H. Tsai, T. Rülicke, S. Hippenmeyer, Oxford Open Neuroscience 1 (2022).","chicago":"Hansen, Andi H, Florian Pauler, Michael Riedl, Carmen Streicher, Anna-Magdalena Heger, Susanne Laukoter, Christoph M Sommer, et al. “Tissue-Wide Effects Override Cell-Intrinsic Gene Function in Radial Neuron Migration.” <i>Oxford Open Neuroscience</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/oons/kvac009\">https://doi.org/10.1093/oons/kvac009</a>."},"date_published":"2022-07-07T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"         1","related_material":{"record":[{"relation":"dissertation_contains","id":"12726","status":"public"},{"relation":"dissertation_contains","id":"14530","status":"public"}]},"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","type":"journal_article","doi":"10.1093/oons/kvac009","date_updated":"2026-04-07T13:29:13Z","volume":1,"quality_controlled":"1","date_created":"2022-02-25T07:52:11Z","_id":"10791","month":"07","oa_version":"Published Version","project":[{"name":"Molecular Mechanisms of Cerebral Cortex Development","_id":"25D61E48-B435-11E9-9278-68D0E5697425","grant_number":"618444","call_identifier":"FP7"},{"grant_number":"24812","name":"Molecular mechanisms of radial neuronal migration","_id":"2625A13E-B435-11E9-9278-68D0E5697425"}]},{"abstract":[{"lang":"eng","text":"Significant advances in the synthesis and processing of colloidal nanocrystals have given scientists and engineers access to a vast library of building blocks with precisely defined size, shape, and composition. These materials have inspired exciting prospects to enable bottom-up fabrication of programmable materials with properties by design. Successfully assembling and connecting the building blocks into superstructures in which constituent nanocrystals can purposefully interact requires robust understanding of and control over a complex interplay of dynamic physicochemical processes. Fluid interfaces provide an advantageous experimental workbench to both probe and control these processes. Despite the ostensible simplicity of fabricating nanocrystal assemblies at a fluid interface, sensitivity to processing conditions and limited reproducibility have underscored the complexity of this process. In situ studies have provided mechanistic insights into the competing dynamics of key subprocesses including solvent spreading and evaporation, superlattice formation, ligand detachment kinetics, and nanocrystal attachment. Understanding how these subprocesses influence the complex choreography of self-assembly, structure transformation, and oriented attachment processes presents a rich research challenge. In this context, we present a detailed methodology for self-assembly and attachment of lead chalcogenide nanocrystals at a liquid–gas interface as a model system for the fabrication of mono- and multilayer cubic connected superlattices. We discuss key experimental parameters such as the characteristics of the building blocks and processing conditions and detailed steps from colloidal nanocrystal injection to superlattice transfer. We hope that this Methods/Protocols paper will provide guidance for future advances in the exciting path toward bringing the prospect of nanocrystal-based programmable materials to fruition."}],"title":"Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface","page":"9457-9472","publication_status":"published","oa":1,"article_processing_charge":"No","issue":"24","day":"16","author":[{"full_name":"Cimada daSilva, Jessica","first_name":"Jessica","last_name":"Cimada daSilva"},{"full_name":"Balazs, Daniel","first_name":"Daniel","orcid":"0000-0001-7597-043X","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","last_name":"Balazs"},{"last_name":"Dunbar","full_name":"Dunbar, Tyler A.","first_name":"Tyler A."},{"first_name":"Tobias","full_name":"Hanrath, Tobias","last_name":"Hanrath"}],"language":[{"iso":"eng"}],"article_type":"original","year":"2021","publication":"Chemistry of Materials","publication_identifier":{"eissn":["1520-5002"],"issn":["0897-4756"]},"volume":33,"date_created":"2024-04-03T07:23:30Z","quality_controlled":"1","date_updated":"2024-04-03T13:50:53Z","month":"12","_id":"15260","oa_version":"Submitted Version","scopus_import":"1","main_file_link":[{"open_access":"1","url":"https://www.osti.gov/servlets/purl/1836502"}],"doi":"10.1021/acs.chemmater.1c02910","type":"journal_article","keyword":["Materials Chemistry","General Chemical Engineering","General Chemistry"],"citation":{"ista":"Cimada daSilva J, Balazs D, Dunbar TA, Hanrath T. 2021. Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface. Chemistry of Materials. 33(24), 9457–9472.","mla":"Cimada daSilva, Jessica, et al. “Fundamental Processes and Practical Considerations of Lead Chalcogenide Mesocrystals Formed via Self-Assembly and Directed Attachment of Nanocrystals at a Fluid Interface.” <i>Chemistry of Materials</i>, vol. 33, no. 24, American Chemical Society, 2021, pp. 9457–72, doi:<a href=\"https://doi.org/10.1021/acs.chemmater.1c02910\">10.1021/acs.chemmater.1c02910</a>.","chicago":"Cimada daSilva, Jessica, Daniel Balazs, Tyler A. Dunbar, and Tobias Hanrath. “Fundamental Processes and Practical Considerations of Lead Chalcogenide Mesocrystals Formed via Self-Assembly and Directed Attachment of Nanocrystals at a Fluid Interface.” <i>Chemistry of Materials</i>. American Chemical Society, 2021. <a href=\"https://doi.org/10.1021/acs.chemmater.1c02910\">https://doi.org/10.1021/acs.chemmater.1c02910</a>.","short":"J. Cimada daSilva, D. Balazs, T.A. Dunbar, T. Hanrath, Chemistry of Materials 33 (2021) 9457–9472.","ama":"Cimada daSilva J, Balazs D, Dunbar TA, Hanrath T. Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface. <i>Chemistry of Materials</i>. 2021;33(24):9457-9472. doi:<a href=\"https://doi.org/10.1021/acs.chemmater.1c02910\">10.1021/acs.chemmater.1c02910</a>","apa":"Cimada daSilva, J., Balazs, D., Dunbar, T. A., &#38; Hanrath, T. (2021). Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface. <i>Chemistry of Materials</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.chemmater.1c02910\">https://doi.org/10.1021/acs.chemmater.1c02910</a>","ieee":"J. Cimada daSilva, D. Balazs, T. A. Dunbar, and T. Hanrath, “Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface,” <i>Chemistry of Materials</i>, vol. 33, no. 24. American Chemical Society, pp. 9457–9472, 2021."},"date_published":"2021-12-16T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"        33","status":"public","department":[{"_id":"LifeSc"}],"publisher":"American Chemical Society"},{"publisher":"Elsevier","department":[{"_id":"MaDe"},{"_id":"LifeSc"}],"file_date_updated":"2021-10-11T12:20:58Z","ec_funded":1,"status":"public","article_number":"101094","citation":{"ista":"Artan M, Barratt S, Flynn SM, Begum F, Skehel M, Nicolas A, de Bono M. 2021. Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. Journal of Biological Chemistry. 297(3), 101094.","mla":"Artan, Murat, et al. “Interactome Analysis of Caenorhabditis Elegans Synapses by TurboID-Based Proximity Labeling.” <i>Journal of Biological Chemistry</i>, vol. 297, no. 3, 101094, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">10.1016/J.JBC.2021.101094</a>.","short":"M. Artan, S. Barratt, S.M. Flynn, F. Begum, M. Skehel, A. Nicolas, M. de Bono, Journal of Biological Chemistry 297 (2021).","chicago":"Artan, Murat, Stephen Barratt, Sean M. Flynn, Farida Begum, Mark Skehel, Armel Nicolas, and Mario de Bono. “Interactome Analysis of Caenorhabditis Elegans Synapses by TurboID-Based Proximity Labeling.” <i>Journal of Biological Chemistry</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">https://doi.org/10.1016/J.JBC.2021.101094</a>.","ama":"Artan M, Barratt S, Flynn SM, et al. Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. <i>Journal of Biological Chemistry</i>. 2021;297(3). doi:<a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">10.1016/J.JBC.2021.101094</a>","apa":"Artan, M., Barratt, S., Flynn, S. M., Begum, F., Skehel, M., Nicolas, A., &#38; de Bono, M. (2021). Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. <i>Journal of Biological Chemistry</i>. Elsevier. <a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">https://doi.org/10.1016/J.JBC.2021.101094</a>","ieee":"M. Artan <i>et al.</i>, “Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling,” <i>Journal of Biological Chemistry</i>, vol. 297, no. 3. Elsevier, 2021."},"date_published":"2021-09-01T00:00:00Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","intvolume":"       297","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"type":"journal_article","doi":"10.1016/J.JBC.2021.101094","quality_controlled":"1","date_updated":"2025-04-14T07:43:46Z","volume":297,"date_created":"2021-10-10T22:01:23Z","_id":"10117","oa_version":"Published Version","month":"09","project":[{"call_identifier":"H2020","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships"}],"scopus_import":"1","publication_identifier":{"eissn":["1083-351X"],"issn":["0021-9258"]},"isi":1,"file":[{"file_name":"2021_JBC_Artan.pdf","file_id":"10121","relation":"main_file","checksum":"19e39d36c5b9387c6dc0e89c9ae856ab","success":1,"creator":"cchlebak","date_updated":"2021-10-11T12:20:58Z","file_size":1680010,"date_created":"2021-10-11T12:20:58Z","content_type":"application/pdf","access_level":"open_access"}],"publication":"Journal of Biological Chemistry","article_type":"original","year":"2021","language":[{"iso":"eng"}],"external_id":{"isi":["000706409200006"]},"issue":"3","day":"01","ddc":["612"],"author":[{"first_name":"Murat","full_name":"Artan, Murat","orcid":"0000-0001-8945-6992","id":"C407B586-6052-11E9-B3AE-7006E6697425","last_name":"Artan"},{"last_name":"Barratt","id":"57740d2b-2a88-11ec-97cf-d9e6d1b39677","first_name":"Stephen","full_name":"Barratt, Stephen"},{"last_name":"Flynn","full_name":"Flynn, Sean M.","first_name":"Sean M."},{"last_name":"Begum","first_name":"Farida","full_name":"Begum, Farida"},{"last_name":"Skehel","first_name":"Mark","full_name":"Skehel, Mark"},{"full_name":"Nicolas, Armel","first_name":"Armel","id":"2A103192-F248-11E8-B48F-1D18A9856A87","last_name":"Nicolas"},{"first_name":"Mario","full_name":"De Bono, Mario","id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"De Bono","orcid":"0000-0001-8347-0443"}],"oa":1,"article_processing_charge":"Yes","publication_status":"published","acknowledgement":"We thank de Bono lab members for helpful comments on the manuscript, IST Austria and University of Vienna Mass Spec Facilities for invaluable discussions and comments for the optimization of mass spec analyses of worm samples. The biotin auxotropic E. coli strain MG1655bioB:kan was gift from John Cronan (University of Illinois) and was kindly sent to us by Jessica Feldman and Ariana Sanchez (Stanford University). dg398 pEntryslot2_mNeongreen::3XFLAG::stop and dg397 pEntryslot3_mNeongreen::3XFLAG::stop::unc-54 3′UTR entry vector were kindly shared by Dr Dominique Glauser (University of Fribourg). Codon-optimized mScarlet vector was a generous gift from Dr Manuel Zimmer (University of Vienna).","abstract":[{"text":"Proximity labeling provides a powerful in vivo tool to characterize the proteome of subcellular structures and the interactome of specific proteins. The nematode Caenorhabditis elegans is one of the most intensely studied organisms in biology, offering many advantages for biochemistry. Using the highly active biotin ligase TurboID, we optimize here a proximity labeling protocol for C. elegans. An advantage of TurboID is that biotin's high affinity for streptavidin means biotin-labeled proteins can be affinity-purified under harsh denaturing conditions. By combining extensive sonication with aggressive denaturation using SDS and urea, we achieved near-complete solubilization of worm proteins. We then used this protocol to characterize the proteomes of the worm gut, muscle, skin, and nervous system. Neurons are among the smallest C. elegans cells. To probe the method's sensitivity, we expressed TurboID exclusively in the two AFD neurons and showed that the protocol could identify known and previously unknown proteins expressed selectively in AFD. The active zones of synapses are composed of a protein matrix that is difficult to solubilize and purify. To test if our protocol could solubilize active zone proteins, we knocked TurboID into the endogenous elks-1 gene, which encodes a presynaptic active zone protein. We identified many known ELKS-1-interacting active zone proteins, as well as previously uncharacterized synaptic proteins. Versatile vectors and the inherent advantages of using C. elegans, including fast growth and the ability to rapidly make and functionally test knock-ins, make proximity labeling a valuable addition to the armory of this model organism.","lang":"eng"}],"title":"Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling"},{"language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2021-06-08T00:00:00Z","citation":{"ieee":"D. Balazs, J. Cimada da Silva, T. Dunbar, M. Ibáñez, and T. Hanrath, “Controlled reactive assembly of colloidal nanocrystal superlattices: Mechanism and kinetics,” in <i>Proceedings of the Internet NanoGe Conference on Nanocrystals</i>, Virtual, 2021.","apa":"Balazs, D., Cimada da Silva, J., Dunbar, T., Ibáñez, M., &#38; Hanrath, T. (2021). Controlled reactive assembly of colloidal nanocrystal superlattices: Mechanism and kinetics. In <i>Proceedings of the Internet NanoGe Conference on Nanocrystals</i>. Virtual: Fundació de la comunitat valenciana SCITO. <a href=\"https://doi.org/10.29363/nanoge.incnc.2021.050\">https://doi.org/10.29363/nanoge.incnc.2021.050</a>","ama":"Balazs D, Cimada da Silva J, Dunbar T, Ibáñez M, Hanrath T. Controlled reactive assembly of colloidal nanocrystal superlattices: Mechanism and kinetics. In: <i>Proceedings of the Internet NanoGe Conference on Nanocrystals</i>. Fundació de la comunitat valenciana SCITO; 2021. doi:<a href=\"https://doi.org/10.29363/nanoge.incnc.2021.050\">10.29363/nanoge.incnc.2021.050</a>","chicago":"Balazs, Daniel, Jessica Cimada da Silva, Tyler Dunbar, Maria Ibáñez, and Tobias Hanrath. “Controlled Reactive Assembly of Colloidal Nanocrystal Superlattices: Mechanism and Kinetics.” In <i>Proceedings of the Internet NanoGe Conference on Nanocrystals</i>. Fundació de la comunitat valenciana SCITO, 2021. <a href=\"https://doi.org/10.29363/nanoge.incnc.2021.050\">https://doi.org/10.29363/nanoge.incnc.2021.050</a>.","short":"D. Balazs, J. Cimada da Silva, T. Dunbar, M. Ibáñez, T. Hanrath, in:, Proceedings of the Internet NanoGe Conference on Nanocrystals, Fundació de la comunitat valenciana SCITO, 2021.","mla":"Balazs, Daniel, et al. “Controlled Reactive Assembly of Colloidal Nanocrystal Superlattices: Mechanism and Kinetics.” <i>Proceedings of the Internet NanoGe Conference on Nanocrystals</i>, 050, Fundació de la comunitat valenciana SCITO, 2021, doi:<a href=\"https://doi.org/10.29363/nanoge.incnc.2021.050\">10.29363/nanoge.incnc.2021.050</a>.","ista":"Balazs D, Cimada da Silva J, Dunbar T, Ibáñez M, Hanrath T. 2021. Controlled reactive assembly of colloidal nanocrystal superlattices: Mechanism and kinetics. Proceedings of the Internet NanoGe Conference on Nanocrystals. iNCNC: Internet nanoGe Conference on Nanocrystals, 050."},"article_number":"050","year":"2021","status":"public","corr_author":"1","department":[{"_id":"MaIb"},{"_id":"LifeSc"}],"publication":"Proceedings of the Internet NanoGe Conference on Nanocrystals","publisher":"Fundació de la comunitat valenciana SCITO","title":"Controlled reactive assembly of colloidal nanocrystal superlattices: Mechanism and kinetics","quality_controlled":"1","date_updated":"2026-07-06T13:07:52Z","date_created":"2024-04-03T08:28:26Z","month":"06","_id":"15280","oa_version":"Published Version","doi":"10.29363/nanoge.incnc.2021.050","main_file_link":[{"url":"https://doi.org/10.29363/nanoge.incnc.2021.050","open_access":"1"}],"publication_status":"published","article_processing_charge":"No","type":"conference_abstract","oa":1,"conference":{"end_date":"2021-07-02","location":"Virtual","name":"iNCNC: Internet nanoGe Conference on Nanocrystals","start_date":"2021-06-28"},"day":"08","ddc":["530"],"author":[{"full_name":"Balazs, Daniel","first_name":"Daniel","orcid":"0000-0001-7597-043X","last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E"},{"first_name":"Jessica","full_name":"Cimada da Silva, Jessica","last_name":"Cimada da Silva"},{"last_name":"Dunbar","full_name":"Dunbar, Tyler","first_name":"Tyler"},{"id":"43C61214-F248-11E8-B48F-1D18A9856A87","last_name":"Ibáñez","orcid":"0000-0001-5013-2843","first_name":"Maria","full_name":"Ibáñez, Maria"},{"last_name":"Hanrath","full_name":"Hanrath, Tobias","first_name":"Tobias"}]},{"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"19557"},{"status":"public","relation":"earlier_version","id":"7800"},{"id":"12401","relation":"dissertation_contains","status":"public"}],"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/defective-gene-slows-down-brain-cells/"}]},"keyword":["General Biochemistry","Genetics and Molecular Biology"],"type":"journal_article","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"doi":"10.1038/s41467-021-23123-x","project":[{"grant_number":"754411","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"grant_number":"715508","call_identifier":"H2020","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","_id":"25444568-B435-11E9-9278-68D0E5697425"},{"name":"Molecular Drug Targets","_id":"2548AE96-B435-11E9-9278-68D0E5697425","grant_number":"W1232","call_identifier":"FWF"},{"grant_number":"F7807","_id":"05A0D778-7A3F-11EA-A408-12923DDC885E","name":"Stem Cell Modulation in Neural Development and Regeneration/ P07-Neural stem cells in autism and epilepsy"},{"grant_number":"I03600","call_identifier":"FWF","name":"Optical control of synaptic function via adhesion molecules","_id":"265CB4D0-B435-11E9-9278-68D0E5697425"}],"scopus_import":"1","quality_controlled":"1","volume":12,"date_updated":"2026-07-24T22:30:10Z","date_created":"2021-05-28T11:49:46Z","oa_version":"Published Version","_id":"9429","month":"05","publisher":"Springer Nature","department":[{"_id":"GaNo"},{"_id":"JoDa"},{"_id":"FlSc"},{"_id":"MiSi"},{"_id":"LifeSc"},{"_id":"Bio"}],"file_date_updated":"2021-05-28T12:39:43Z","article_number":"3058","ec_funded":1,"status":"public","corr_author":"1","intvolume":"        12","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_published":"2021-05-24T00:00:00Z","citation":{"ista":"Morandell J, Schwarz LA, Basilico B, Tasciyan S, Dimchev GA, Nicolas A, Sommer CM, Kreuzinger C, Dotter C, Knaus L, Dobler Z, Cacci E, Schur FK, Danzl JG, Novarino G. 2021. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nature Communications. 12(1), 3058.","chicago":"Morandell, Jasmin, Lena A Schwarz, Bernadette Basilico, Saren Tasciyan, Georgi A Dimchev, Armel Nicolas, Christoph M Sommer, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-23123-x\">https://doi.org/10.1038/s41467-021-23123-x</a>.","short":"J. Morandell, L.A. Schwarz, B. Basilico, S. Tasciyan, G.A. Dimchev, A. Nicolas, C.M. Sommer, C. Kreuzinger, C. Dotter, L. Knaus, Z. Dobler, E. Cacci, F.K. Schur, J.G. Danzl, G. Novarino, Nature Communications 12 (2021).","mla":"Morandell, Jasmin, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>Nature Communications</i>, vol. 12, no. 1, 3058, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-23123-x\">10.1038/s41467-021-23123-x</a>.","ama":"Morandell J, Schwarz LA, Basilico B, et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>Nature Communications</i>. 2021;12(1). doi:<a href=\"https://doi.org/10.1038/s41467-021-23123-x\">10.1038/s41467-021-23123-x</a>","ieee":"J. Morandell <i>et al.</i>, “Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development,” <i>Nature Communications</i>, vol. 12, no. 1. Springer Nature, 2021.","apa":"Morandell, J., Schwarz, L. A., Basilico, B., Tasciyan, S., Dimchev, G. A., Nicolas, A., … Novarino, G. (2021). Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-23123-x\">https://doi.org/10.1038/s41467-021-23123-x</a>"},"ddc":["572"],"day":"24","author":[{"id":"4739D480-F248-11E8-B48F-1D18A9856A87","last_name":"Morandell","full_name":"Morandell, Jasmin","first_name":"Jasmin"},{"first_name":"Lena A","full_name":"Schwarz, Lena A","id":"29A8453C-F248-11E8-B48F-1D18A9856A87","last_name":"Schwarz"},{"last_name":"Basilico","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","orcid":"0000-0003-1843-3173","full_name":"Basilico, Bernadette","first_name":"Bernadette"},{"id":"4323B49C-F248-11E8-B48F-1D18A9856A87","last_name":"Tasciyan","orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren","first_name":"Saren"},{"first_name":"Georgi A","full_name":"Dimchev, Georgi A","last_name":"Dimchev","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8370-6161"},{"first_name":"Armel","full_name":"Nicolas, Armel","id":"2A103192-F248-11E8-B48F-1D18A9856A87","last_name":"Nicolas"},{"orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","full_name":"Sommer, Christoph M","first_name":"Christoph M"},{"first_name":"Caroline","full_name":"Kreuzinger, Caroline","last_name":"Kreuzinger","id":"382077BA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Christoph","full_name":"Dotter, Christoph","orcid":"0000-0002-9033-9096","id":"4C66542E-F248-11E8-B48F-1D18A9856A87","last_name":"Dotter"},{"id":"3B2ABCF4-F248-11E8-B48F-1D18A9856A87","last_name":"Knaus","first_name":"Lisa","full_name":"Knaus, Lisa"},{"id":"D23090A2-9057-11EA-883A-A8396FC7A38F","last_name":"Dobler","first_name":"Zoe","full_name":"Dobler, Zoe"},{"last_name":"Cacci","full_name":"Cacci, Emanuele","first_name":"Emanuele"},{"orcid":"0000-0003-4790-8078","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","last_name":"Schur","full_name":"Schur, Florian KM","first_name":"Florian KM"},{"first_name":"Johann G","full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl"},{"full_name":"Novarino, Gaia","first_name":"Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","last_name":"Novarino","orcid":"0000-0002-7673-7178"}],"issue":"1","article_processing_charge":"No","oa":1,"acknowledgement":"We thank A. Coll Manzano, F. Freeman, M. Ladron de Guevara, and A. Ç. Yahya for technical assistance, S. Deixler, A. Lepold, and A. Schlerka for the management of our animal colony, as well as M. Schunn and the Preclinical Facility team for technical assistance. We thank K. Heesom and her team at the University of Bristol Proteomics Facility for the proteomics sample preparation, data generation, and analysis support. We thank Y. B. Simon for kindly providing the plasmid for lentiviral labeling. Further, we thank M. Sixt for his advice regarding cell migration and the fruitful discussions. This work was supported by the ISTPlus postdoctoral fellowship (Grant Agreement No. 754411) to B.B., by the European Union’s Horizon 2020 research and innovation program (ERC) grant 715508 (REVERSEAUTISM), and by the Austrian Science Fund (FWF) to G.N. (DK W1232-B24 and SFB F7807-B) and to J.G.D (I3600-B27).","publication_status":"published","title":"Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development","abstract":[{"lang":"eng","text":"De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3 lead to autism spectrum disorder (ASD). In mouse, constitutive haploinsufficiency leads to motor coordination deficits as well as ASD-relevant social and cognitive impairments. However, induction of Cul3 haploinsufficiency later in life does not lead to ASD-relevant behaviors, pointing to an important role of Cul3 during a critical developmental window. Here we show that Cul3 is essential to regulate neuronal migration and, therefore, constitutive Cul3 heterozygous mutant mice display cortical lamination abnormalities. At the molecular level, we found that Cul3 controls neuronal migration by tightly regulating the amount of Plastin3 (Pls3), a previously unrecognized player of neural migration. Furthermore, we found that Pls3 cell-autonomously regulates cell migration by regulating actin cytoskeleton organization, and its levels are inversely proportional to neural migration speed. Finally, we provide evidence that cellular phenotypes associated with autism-linked gene haploinsufficiency can be rescued by transcriptional activation of the intact allele in vitro, offering a proof of concept for a potential therapeutic approach for ASDs."}],"acknowledged_ssus":[{"_id":"PreCl"}],"isi":1,"file":[{"checksum":"337e0f7959c35ec959984cacdcb472ba","file_name":"2021_NatureCommunications_Morandell.pdf","file_id":"9430","relation":"main_file","content_type":"application/pdf","access_level":"open_access","creator":"kschuh","success":1,"date_created":"2021-05-28T12:39:43Z","file_size":9358599,"date_updated":"2021-05-28T12:39:43Z"}],"publication_identifier":{"eissn":["2041-1723"]},"publication":"Nature Communications","article_type":"original","year":"2021","language":[{"iso":"eng"}],"external_id":{"isi":["000658769900010"]}},{"acknowledged_ssus":[{"_id":"PreCl"}],"file":[{"checksum":"c6799ab5daba80efe8e2ed63c15f8c81","file_name":"2020.01.10.902064v1.full.pdf","file_id":"7801","relation":"main_file","content_type":"application/pdf","access_level":"open_access","creator":"rsix","file_size":2931370,"date_updated":"2020-07-14T12:48:03Z","date_created":"2020-05-05T14:31:19Z"}],"das_tickbox":"1","publication":"bioRxiv","year":"2020","language":[{"iso":"eng"}],"day":"11","ddc":["570"],"author":[{"full_name":"Morandell, Jasmin","first_name":"Jasmin","id":"4739D480-F248-11E8-B48F-1D18A9856A87","last_name":"Morandell"},{"last_name":"Schwarz","id":"29A8453C-F248-11E8-B48F-1D18A9856A87","full_name":"Schwarz, Lena A","first_name":"Lena A"},{"orcid":"0000-0003-1843-3173","last_name":"Basilico","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","first_name":"Bernadette","full_name":"Basilico, Bernadette"},{"first_name":"Saren","full_name":"Tasciyan, Saren","last_name":"Tasciyan","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1671-393X"},{"id":"2A103192-F248-11E8-B48F-1D18A9856A87","last_name":"Nicolas","full_name":"Nicolas, Armel","first_name":"Armel"},{"full_name":"Sommer, Christoph M","first_name":"Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105"},{"first_name":"Caroline","full_name":"Kreuzinger, Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87","last_name":"Kreuzinger"},{"full_name":"Knaus, Lisa","first_name":"Lisa","last_name":"Knaus","id":"3B2ABCF4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Zoe","full_name":"Dobler, Zoe","last_name":"Dobler","id":"D23090A2-9057-11EA-883A-A8396FC7A38F"},{"last_name":"Cacci","full_name":"Cacci, Emanuele","first_name":"Emanuele"},{"orcid":"0000-0001-8559-3973","last_name":"Danzl","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","first_name":"Johann G","full_name":"Danzl, Johann G"},{"orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","last_name":"Novarino","first_name":"Gaia","full_name":"Novarino, Gaia"}],"article_processing_charge":"No","oa":1,"publication_status":"draft","title":"Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development","abstract":[{"lang":"eng","text":"De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3 (CUL3) lead to autism spectrum disorder (ASD). Here, we used Cul3 mouse models to evaluate the consequences of Cul3 mutations in vivo. Our results show that Cul3 haploinsufficient mice exhibit deficits in motor coordination as well as ASD-relevant social and cognitive impairments. Cul3 mutant brain displays cortical lamination abnormalities due to defective neuronal migration and reduced numbers of excitatory and inhibitory neurons. In line with the observed abnormal columnar organization, Cul3 haploinsufficiency is associated with decreased spontaneous excitatory and inhibitory activity in the cortex. At the molecular level, employing a quantitative proteomic approach, we show that Cul3 regulates cytoskeletal and adhesion protein abundance in mouse embryos. Abnormal regulation of cytoskeletal proteins in Cul3 mutant neuronal cells results in atypical organization of the actin mesh at the cell leading edge, likely causing the observed migration deficits. In contrast to these important functions early in development, Cul3 deficiency appears less relevant at adult stages. In fact, induction of Cul3 haploinsufficiency in adult mice does not result in the behavioral defects observed in constitutive Cul3 haploinsufficient animals. Taken together, our data indicate that Cul3 has a critical role in the regulation of cytoskeletal proteins and neuronal migration and that ASD-associated defects and behavioral abnormalities are primarily due to Cul3 functions at early developmental stages."}],"department":[{"_id":"JoDa"},{"_id":"GaNo"},{"_id":"LifeSc"}],"file_date_updated":"2020-07-14T12:48:03Z","status":"public","corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2020-01-11T00:00:00Z","citation":{"ama":"Morandell J, Schwarz LA, Basilico B, et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2020.01.10.902064 \">10.1101/2020.01.10.902064 </a>","ieee":"J. Morandell <i>et al.</i>, “Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development,” <i>bioRxiv</i>. .","apa":"Morandell, J., Schwarz, L. A., Basilico, B., Tasciyan, S., Nicolas, A., Sommer, C. M., … Novarino, G. (n.d.). Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2020.01.10.902064 \">https://doi.org/10.1101/2020.01.10.902064 </a>","ista":"Morandell J, Schwarz LA, Basilico B, Tasciyan S, Nicolas A, Sommer CM, Kreuzinger C, Knaus L, Dobler Z, Cacci E, Danzl JG, Novarino G. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. bioRxiv, <a href=\"https://doi.org/10.1101/2020.01.10.902064 \">10.1101/2020.01.10.902064 </a>.","short":"J. Morandell, L.A. Schwarz, B. Basilico, S. Tasciyan, A. Nicolas, C.M. Sommer, C. Kreuzinger, L. Knaus, Z. Dobler, E. Cacci, J.G. Danzl, G. Novarino, BioRxiv (n.d.).","chicago":"Morandell, Jasmin, Lena A Schwarz, Bernadette Basilico, Saren Tasciyan, Armel Nicolas, Christoph M Sommer, Caroline Kreuzinger, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2020.01.10.902064 \">https://doi.org/10.1101/2020.01.10.902064 </a>.","mla":"Morandell, Jasmin, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2020.01.10.902064 \">10.1101/2020.01.10.902064 </a>."},"related_material":{"record":[{"status":"public","id":"9429","relation":"later_version"},{"status":"public","relation":"dissertation_contains","id":"8620"}]},"type":"preprint","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"doi":"10.1101/2020.01.10.902064 ","project":[{"grant_number":"I03600","call_identifier":"FWF","name":"Optical control of synaptic function via adhesion molecules","_id":"265CB4D0-B435-11E9-9278-68D0E5697425"},{"name":"Molecular Drug Targets","_id":"2548AE96-B435-11E9-9278-68D0E5697425","grant_number":"W1232","call_identifier":"FWF"}],"date_created":"2020-05-05T14:31:33Z","date_updated":"2026-07-24T22:31:01Z","_id":"7800","month":"01","oa_version":"Preprint"}]