---
OA_place: publisher
OA_type: hybrid
_id: '21264'
abstract:
- lang: eng
text: Rodents' ability to encode the whisking phase has been extensively documented
through neuronal recordings from ascending sensory pathways. Yet, while indicating
that reafference originates from the mechanoreceptors, the mechanistic underpinnings
of the whisking phase encoding within the follicle remain unclear. Here we present
anatomical, histological, and biomechanical evidence for the presence of a distinctive
elastic segment (ES) within the basal part of the whisker shaft inside the follicle.
This ES, composed of immature keratin, is capable of both bending and twisting.
Forces generated by whisker movement deform this segment, causing whisker shaft
deflections that can stimulate specific mechanoreceptor subsets within the follicle
at different phases of the whisking cycle. This mechanism appears to operate during
both free‐air whisking and object contact. We propose that the ES enables torsion‐based
mechanoreceptor activation, allowing encoding of the whisking phase.
acknowledgement: The authors wish to express their gratitude to Prof. Menahem Segal
and Dr. Yonatan Katz for their helpful comments and discussions. The United States-Israel
Binational Science Foundation (BSF, grant no. 2021327); The European Research Council
(ERC) under the EU Horizon 2020 Research and Innovation Programme (grant no. 786949);
the Israel Science Foundation (ISF, grant no. 2237/20); The Weizmann-UK Collaboration
and a research grant from the Estate of Thomas Gruen.
article_number: ar.70051
article_processing_charge: No
article_type: original
author:
- first_name: Sebastian
full_name: Haidarliu, Sebastian
last_name: Haidarliu
- first_name: Guy
full_name: Nelinger, Guy
last_name: Nelinger
- first_name: Luka
full_name: Gantar, Luka
id: ed7c4564-13aa-11f0-9846-960f9afb2ddb
last_name: Gantar
- first_name: Ehud
full_name: Ahissar, Ehud
last_name: Ahissar
- first_name: Inbar
full_name: Saraf‐Sinik, Inbar
last_name: Saraf‐Sinik
citation:
ama: Haidarliu S, Nelinger G, Gantar L, Ahissar E, Saraf‐Sinik I. An elastic segment
of the whisker shaft enables coding of the whisking phase via whisker torsion
in rats and mice. The Anatomical Record. 2025. doi:10.1002/ar.70051
apa: Haidarliu, S., Nelinger, G., Gantar, L., Ahissar, E., & Saraf‐Sinik, I.
(2025). An elastic segment of the whisker shaft enables coding of the whisking
phase via whisker torsion in rats and mice. The Anatomical Record. Wiley.
https://doi.org/10.1002/ar.70051
chicago: Haidarliu, Sebastian, Guy Nelinger, Luka Gantar, Ehud Ahissar, and Inbar
Saraf‐Sinik. “An Elastic Segment of the Whisker Shaft Enables Coding of the Whisking
Phase via Whisker Torsion in Rats and Mice.” The Anatomical Record. Wiley,
2025. https://doi.org/10.1002/ar.70051.
ieee: S. Haidarliu, G. Nelinger, L. Gantar, E. Ahissar, and I. Saraf‐Sinik, “An
elastic segment of the whisker shaft enables coding of the whisking phase via
whisker torsion in rats and mice,” The Anatomical Record. Wiley, 2025.
ista: Haidarliu S, Nelinger G, Gantar L, Ahissar E, Saraf‐Sinik I. 2025. An elastic
segment of the whisker shaft enables coding of the whisking phase via whisker
torsion in rats and mice. The Anatomical Record., ar. 70051.
mla: Haidarliu, Sebastian, et al. “An Elastic Segment of the Whisker Shaft Enables
Coding of the Whisking Phase via Whisker Torsion in Rats and Mice.” The Anatomical
Record, ar. 70051, Wiley, 2025, doi:10.1002/ar.70051.
short: S. Haidarliu, G. Nelinger, L. Gantar, E. Ahissar, I. Saraf‐Sinik, The Anatomical
Record (2025).
date_created: 2026-02-17T07:44:23Z
date_published: 2025-09-09T00:00:00Z
date_updated: 2026-02-23T10:50:27Z
day: '09'
ddc:
- '570'
department:
- _id: MaJö
doi: 10.1002/ar.70051
external_id:
pmid:
- '40923214'
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.1002/ar.70051
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: The Anatomical Record
publication_identifier:
eissn:
- 1932-8494
issn:
- 1932-8486
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: An elastic segment of the whisker shaft enables coding of the whisking phase
via whisker torsion in rats and mice
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
_id: '19076'
abstract:
- lang: eng
text: For accurate perception and motor control, an animal must distinguish between
sensory experiences elicited by external stimuli and those elicited by its own
actions. The diversity of behaviors and their complex influences on the senses
make this distinction challenging. Here, we uncover an action–cue hub that coordinates
motor commands with visual processing in the brain’s first visual relay. We show
that the ventral lateral geniculate nucleus (vLGN) acts as a corollary discharge
center, integrating visual translational optic flow signals with motor copies
from saccades, locomotion and pupil dynamics. The vLGN relays these signals to
correct action-specific visual distortions and to refine perception, as shown
for the superior colliculus and in a depth-estimation task. Simultaneously, brain-wide
vLGN projections drive corrective actions necessary for accurate visuomotor control.
Our results reveal an extended corollary discharge architecture that refines early
visual transformations and coordinates actions via a distributed hub-and-spoke
network to enable visual perception during action.
acknowledged_ssus:
- _id: ScienComp
- _id: PreCl
- _id: LifeSc
- _id: Bio
acknowledgement: We thank Y. Ben-Simon for generously making viral vectors for retrograde
tracing available, as well as J. Watson and F. Marr for reagents. We also thank
R. Shigemoto, W. Młynarski and members of the Neuroethology group for their comments
on the manuscript and L. Burnett for her schematic drawings. This research was supported
by the Scientific Service Units of ISTA through resources provided by Scientific
Computing, the Preclinical Facility, the Lab Support Facility and the Imaging and
Optics Facility, in particular F. Lange, M. Schunn and T. Asenov. This work was
supported by European Research Council Starting Grant no. 756502 (M.J.) and European
Research Council Consolidator Grant no. 101086580 (M.J.); and EMBO ALTF grant no.
1098-2017 (A.S.) and Human Frontiers Science Program grant no. LT000256/2018-L (A.S.).
Open access funding provided by Institute of Science and Technology (IST Austria).
article_number: '7278'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Anton L
full_name: Sumser, Anton L
id: 3320A096-F248-11E8-B48F-1D18A9856A87
last_name: Sumser
orcid: 0000-0002-4792-1881
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Florian
full_name: Schmidt, Florian
id: A2EF226A-AF19-11E9-924C-0525E6697425
last_name: Schmidt
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA.
A thalamic hub-and-spoke network enables visual perception during action by coordinating
visuomotor dynamics. Nature Neuroscience. 2025;28. doi:10.1038/s41593-025-01874-w
apa: Vega Zuniga, T. A., Sumser, A. L., Symonova, O., Koppensteiner, P., Schmidt,
F., & Jösch, M. A. (2025). A thalamic hub-and-spoke network enables visual
perception during action by coordinating visuomotor dynamics. Nature Neuroscience.
Springer Nature. https://doi.org/10.1038/s41593-025-01874-w
chicago: Vega Zuniga, Tomas A, Anton L Sumser, Olga Symonova, Peter Koppensteiner,
Florian Schmidt, and Maximilian A Jösch. “A Thalamic Hub-and-Spoke Network Enables
Visual Perception during Action by Coordinating Visuomotor Dynamics.” Nature
Neuroscience. Springer Nature, 2025. https://doi.org/10.1038/s41593-025-01874-w.
ieee: T. A. Vega Zuniga, A. L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt,
and M. A. Jösch, “A thalamic hub-and-spoke network enables visual perception during
action by coordinating visuomotor dynamics,” Nature Neuroscience, vol.
28. Springer Nature, 2025.
ista: Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA.
2025. A thalamic hub-and-spoke network enables visual perception during action
by coordinating visuomotor dynamics. Nature Neuroscience. 28, 7278.
mla: Vega Zuniga, Tomas A., et al. “A Thalamic Hub-and-Spoke Network Enables Visual
Perception during Action by Coordinating Visuomotor Dynamics.” Nature Neuroscience,
vol. 28, 7278, Springer Nature, 2025, doi:10.1038/s41593-025-01874-w.
short: T.A. Vega Zuniga, A.L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt,
M.A. Jösch, Nature Neuroscience 28 (2025).
corr_author: '1'
date_created: 2025-02-23T23:01:58Z
date_published: 2025-03-01T00:00:00Z
date_updated: 2026-06-18T18:12:08Z
day: '01'
ddc:
- '570'
department:
- _id: MaJö
- _id: PreCl
doi: 10.1038/s41593-025-01874-w
ec_funded: 1
external_id:
isi:
- '001416866800001'
pmid:
- '39930095'
has_accepted_license: '1'
intvolume: ' 28'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41593-025-01874-w
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
- _id: bdaf81a8-d553-11ed-ba76-c95961984540
grant_number: '101086580'
name: 'Action Selection in the Midbrain: Neuromodulation of Visuomotor Senses'
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1098-2017
name: Connecting sensory with motor processing in the superior colliculus
- _id: 266D407A-B435-11E9-9278-68D0E5697425
grant_number: LT000256
name: Neuronal networks of salience and spatial detection in the murine superior
colliculus
publication: Nature Neuroscience
publication_identifier:
eissn:
- 1546-1726
issn:
- 1097-6256
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/high-tech-video-optimization-in-our-brain/
record:
- id: '18579'
relation: research_data
status: public
scopus_import: '1'
status: public
title: A thalamic hub-and-spoke network enables visual perception during action by
coordinating visuomotor dynamics
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 28
year: '2025'
...
---
_id: '15385'
abstract:
- lang: eng
text: "Relevant information about the data can be found in the 'Readme_Data.txt'
file. \r\nA previous version of the publication can be found on BioRxiv: https://www.biorxiv.org/content/10.1101/2022.10.11.511691v4\r\nand
published in Plos Biology (2024)"
acknowledged_ssus:
- _id: PreCl
- _id: M-Shop
- _id: LifeSc
- _id: Bio
acknowledgement: 'We thank Armel Nicolas, Bella Bruszel and Ewelina Dutkiewicz from
the ISTA Mass Spectrometry Service (Lab Services Facilities) for all Proteomics
work, including samples preparation, LC/MS data acquisition, searches and data evaluation.
We thank Prof. Peter Jonas for his suggestion on the involvement of potassium channels
and members of the Neuroethology group for their comments on the manuscript. Katalin
Szigeti and Julie Murmann for experimental help. This research was supported by
the Scientific Service Units of ISTA through resources provided by the Lab Support
Facility, the Imaging and Optics Facility, the Machine Shop Unit and the Preclinical
Facility, especially Freyja Langer and Michael Schunn. '
article_processing_charge: No
author:
- first_name: Laura
full_name: Burnett, Laura
id: 3B717F68-F248-11E8-B48F-1D18A9856A87
last_name: Burnett
orcid: 0000-0002-8937-410X
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Tomas
full_name: Masson, Tomas
id: 93ac43e8-8599-11eb-9b86-f6efb0a4c207
last_name: Masson
orcid: 0000-0002-2634-6283
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Ximena
full_name: Contreras, Ximena
id: 475990FE-F248-11E8-B48F-1D18A9856A87
last_name: Contreras
- first_name: Thomas
full_name: Rülicke, Thomas
last_name: Rülicke
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Burnett L, Koppensteiner P, Symonova O, et al. Shared behavioural impairments
in visual perception and place avoidance across different autism models are driven
by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice. 2024.
doi:10.15479/AT:ISTA:15385
apa: Burnett, L., Koppensteiner, P., Symonova, O., Masson, T., Vega Zuniga, T. A.,
Contreras, X., … Jösch, M. A. (2024). Shared behavioural impairments in visual
perception and place avoidance across different autism models are driven by periaqueductal
grey hypoexcitability in Setd5 haploinsufficient mice. Institute of Science and
Technology Austria. https://doi.org/10.15479/AT:ISTA:15385
chicago: Burnett, Laura, Peter Koppensteiner, Olga Symonova, Tomas Masson, Tomas
A Vega Zuniga, Ximena Contreras, Thomas Rülicke, Ryuichi Shigemoto, Gaia Novarino,
and Maximilian A Jösch. “Shared Behavioural Impairments in Visual Perception and
Place Avoidance across Different Autism Models Are Driven by Periaqueductal Grey
Hypoexcitability in Setd5 Haploinsufficient Mice.” Institute of Science and Technology
Austria, 2024. https://doi.org/10.15479/AT:ISTA:15385.
ieee: L. Burnett et al., “Shared behavioural impairments in visual perception
and place avoidance across different autism models are driven by periaqueductal
grey hypoexcitability in Setd5 haploinsufficient mice.” Institute of Science and
Technology Austria, 2024.
ista: Burnett L, Koppensteiner P, Symonova O, Masson T, Vega Zuniga TA, Contreras
X, Rülicke T, Shigemoto R, Novarino G, Jösch MA. 2024. Shared behavioural impairments
in visual perception and place avoidance across different autism models are driven
by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:15385.
mla: Burnett, Laura, et al. Shared Behavioural Impairments in Visual Perception
and Place Avoidance across Different Autism Models Are Driven by Periaqueductal
Grey Hypoexcitability in Setd5 Haploinsufficient Mice. Institute of Science
and Technology Austria, 2024, doi:10.15479/AT:ISTA:15385.
short: L. Burnett, P. Koppensteiner, O. Symonova, T. Masson, T.A. Vega Zuniga, X.
Contreras, T. Rülicke, R. Shigemoto, G. Novarino, M.A. Jösch, (2024).
corr_author: '1'
date_created: 2024-05-13T15:04:04Z
date_published: 2024-05-15T00:00:00Z
date_updated: 2025-09-08T07:57:11Z
day: '15'
ddc:
- '570'
department:
- _id: MaJö
- _id: PreCl
- _id: SiHi
- _id: RySh
- _id: GaNo
doi: 10.15479/AT:ISTA:15385
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has_accepted_license: '1'
keyword:
- ASD
- periaqueductal gray
- perception
- behavior
- potassium channels
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '05'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '17142'
relation: used_in_publication
status: public
status: public
title: Shared behavioural impairments in visual perception and place avoidance across
different autism models are driven by periaqueductal grey hypoexcitability in Setd5
haploinsufficient mice
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: research_data
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2024'
...
---
OA_place: repository
OA_type: green
_id: '15404'
abstract:
- lang: eng
text: We used diverse methods to characterize the role of avian lateral spiriform
nucleus (SpL) in basal ganglia motor function. Connectivity analysis showed that
SpL receives input from globus pallidus (GP), and the intrapeduncular nucleus
(INP) located ventromedial to GP, whose neurons express numerous striatal markers.
SpL-projecting GP neurons were large and aspiny, while SpL-projecting INP neurons
were medium sized and spiny. Connectivity analysis further showed that SpL receives
inputs from subthalamic nucleus (STN) and substantia nigra pars reticulata (SNr),
and that the SNr also receives inputs from GP, INP, and STN. Neurochemical analysis
showed that SpL neurons express ENK, GAD, and a variety of pallidal neuron markers,
and receive GABAergic terminals, some of which also contain DARPP32, consistent
with GP pallidal and INP striatal inputs. Connectivity and neurochemical analysis
showed that the SpL input to tectum prominently ends on GABAA receptor-enriched
tectobulbar neurons. Behavioral studies showed that lesions of SpL impair visuomotor
behaviors involving tracking and pecking moving targets. Our results suggest that
SpL modulates brainstem-projecting tectobulbar neurons in a manner comparable
to the demonstrated influence of GP internus on motor thalamus and of SNr on tectobulbar
neurons in mammals. Given published data in amphibians and reptiles, it seems
likely the SpL circuit represents a major direct pathway-type circuit by which
the basal ganglia exerts its motor influence in nonmammalian tetrapods. The present
studies also show that avian striatum is divided into three spatially segregated
territories with differing connectivity, a medial striato-nigral territory, a
dorsolateral striato-GP territory, and the ventrolateral INP motor territory.
acknowledgement: We gratefully thank Marion Joni, Tony Laverghetta, Sherry Cuthbertson,
Gary Henderson, and Patricia Lindaman for technical assistance. The research presented
here has been supported by NIH grants NS-16857, NS-19620, NS-28721, and EY-05298,
and The Methodist Hospitals Endowed Professorship in Neuroscience (A. R.), by grant
number 09/50623-9 from the Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
(C. A. B. T.), by NIH grant EY-00735 (W. H.), and by NIH grants NS-12078 and EY-02145
(H. J. K.).
article_number: e25620
article_processing_charge: No
article_type: original
author:
- first_name: Anton
full_name: Reiner, Anton
last_name: Reiner
- first_name: Loreta
full_name: Medina, Loreta
last_name: Medina
- first_name: Antonio
full_name: Abellan, Antonio
last_name: Abellan
- first_name: Yunping
full_name: Deng, Yunping
last_name: Deng
- first_name: Claudio A.B.
full_name: Toledo, Claudio A.B.
last_name: Toledo
- first_name: Harald
full_name: Luksch, Harald
last_name: Luksch
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Nell B.
full_name: Riley, Nell B.
last_name: Riley
- first_name: William
full_name: Hodos, William
last_name: Hodos
- first_name: Harvey J.
full_name: Karten, Harvey J.
last_name: Karten
citation:
ama: 'Reiner A, Medina L, Abellan A, et al. Neurochemistry and circuit organization
of the lateral spiriform nucleus of birds: A uniquely nonmammalian direct pathway
component of the basal ganglia. Journal of Comparative Neurology. 2024;532(5).
doi:10.1002/cne.25620'
apa: 'Reiner, A., Medina, L., Abellan, A., Deng, Y., Toledo, C. A. B., Luksch, H.,
… Karten, H. J. (2024). Neurochemistry and circuit organization of the lateral
spiriform nucleus of birds: A uniquely nonmammalian direct pathway component of
the basal ganglia. Journal of Comparative Neurology. Wiley. https://doi.org/10.1002/cne.25620'
chicago: 'Reiner, Anton, Loreta Medina, Antonio Abellan, Yunping Deng, Claudio A.B.
Toledo, Harald Luksch, Tomas A Vega Zuniga, Nell B. Riley, William Hodos, and
Harvey J. Karten. “Neurochemistry and Circuit Organization of the Lateral Spiriform
Nucleus of Birds: A Uniquely Nonmammalian Direct Pathway Component of the Basal
Ganglia.” Journal of Comparative Neurology. Wiley, 2024. https://doi.org/10.1002/cne.25620.'
ieee: 'A. Reiner et al., “Neurochemistry and circuit organization of the
lateral spiriform nucleus of birds: A uniquely nonmammalian direct pathway component
of the basal ganglia,” Journal of Comparative Neurology, vol. 532, no.
5. Wiley, 2024.'
ista: 'Reiner A, Medina L, Abellan A, Deng Y, Toledo CAB, Luksch H, Vega Zuniga
TA, Riley NB, Hodos W, Karten HJ. 2024. Neurochemistry and circuit organization
of the lateral spiriform nucleus of birds: A uniquely nonmammalian direct pathway
component of the basal ganglia. Journal of Comparative Neurology. 532(5), e25620.'
mla: 'Reiner, Anton, et al. “Neurochemistry and Circuit Organization of the Lateral
Spiriform Nucleus of Birds: A Uniquely Nonmammalian Direct Pathway Component of
the Basal Ganglia.” Journal of Comparative Neurology, vol. 532, no. 5,
e25620, Wiley, 2024, doi:10.1002/cne.25620.'
short: A. Reiner, L. Medina, A. Abellan, Y. Deng, C.A.B. Toledo, H. Luksch, T.A.
Vega Zuniga, N.B. Riley, W. Hodos, H.J. Karten, Journal of Comparative Neurology
532 (2024).
date_created: 2024-05-19T22:01:12Z
date_published: 2024-05-01T00:00:00Z
date_updated: 2025-09-08T07:29:27Z
day: '01'
department:
- _id: MaJö
doi: 10.1002/cne.25620
external_id:
isi:
- '001217825300001'
pmid:
- '38733146'
intvolume: ' 532'
isi: 1
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/11090467
month: '05'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
eissn:
- 1096-9861
issn:
- 0021-9967
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Neurochemistry and circuit organization of the lateral spiriform nucleus of
birds: A uniquely nonmammalian direct pathway component of the basal ganglia'
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 532
year: '2024'
...
---
APC_amount: 6081,83 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '17142'
abstract:
- lang: eng
text: Despite the diverse genetic origins of autism spectrum disorders (ASDs), affected
individuals share strikingly similar and correlated behavioural traits that include
perceptual and sensory processing challenges. Notably, the severity of these sensory
symptoms is often predictive of the expression of other autistic traits. However,
the origin of these perceptual deficits remains largely elusive. Here, we show
a recurrent impairment in visual threat perception that is similarly impaired
in 3 independent mouse models of ASD with different molecular aetiologies. Interestingly,
this deficit is associated with reduced avoidance of threatening environments—a
nonperceptual trait. Focusing on a common cause of ASDs, the Setd5 gene mutation,
we define the molecular mechanism. We show that the perceptual impairment is caused
by a potassium channel (Kv1)-mediated hypoexcitability in a subcortical node essential
for the initiation of escape responses, the dorsal periaqueductal grey (dPAG).
Targeted pharmacological Kv1 blockade rescued both perceptual and place avoidance
deficits, causally linking seemingly unrelated trait deficits to the dPAG. Furthermore,
we show that different molecular mechanisms converge on similar behavioural phenotypes
by demonstrating that the autism models Cul3 and Ptchd1, despite having similar
behavioural phenotypes, differ in their functional and molecular alteration. Our
findings reveal a link between rapid perception controlled by subcortical pathways
and appropriate learned interactions with the environment and define a nondevelopmental
source of such deficits in ASD.
acknowledgement: 'This work was supported by a European Research Council Starting
Grant 756502 (MJ). '
article_number: e3002668
article_processing_charge: Yes
article_type: original
author:
- first_name: Laura
full_name: Burnett, Laura
id: 3B717F68-F248-11E8-B48F-1D18A9856A87
last_name: Burnett
orcid: 0000-0002-8937-410X
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Tomas
full_name: Masson, Tomas
id: 93ac43e8-8599-11eb-9b86-f6efb0a4c207
last_name: Masson
orcid: 0000-0002-2634-6283
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Ximena
full_name: Contreras, Ximena
id: 475990FE-F248-11E8-B48F-1D18A9856A87
last_name: Contreras
- first_name: Thomas
full_name: Rülicke, Thomas
last_name: Rülicke
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Burnett L, Koppensteiner P, Symonova O, et al. Shared behavioural impairments
in visual perception and place avoidance across different autism models are driven
by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice. PLoS
Biology. 2024;22. doi:10.1371/journal.pbio.3002668
apa: Burnett, L., Koppensteiner, P., Symonova, O., Masson, T., Vega Zuniga, T. A.,
Contreras, X., … Jösch, M. A. (2024). Shared behavioural impairments in visual
perception and place avoidance across different autism models are driven by periaqueductal
grey hypoexcitability in Setd5 haploinsufficient mice. PLoS Biology. Public
Library of Science. https://doi.org/10.1371/journal.pbio.3002668
chicago: Burnett, Laura, Peter Koppensteiner, Olga Symonova, Tomas Masson, Tomas
A Vega Zuniga, Ximena Contreras, Thomas Rülicke, Ryuichi Shigemoto, Gaia Novarino,
and Maximilian A Jösch. “Shared Behavioural Impairments in Visual Perception and
Place Avoidance across Different Autism Models Are Driven by Periaqueductal Grey
Hypoexcitability in Setd5 Haploinsufficient Mice.” PLoS Biology. Public
Library of Science, 2024. https://doi.org/10.1371/journal.pbio.3002668.
ieee: L. Burnett et al., “Shared behavioural impairments in visual perception
and place avoidance across different autism models are driven by periaqueductal
grey hypoexcitability in Setd5 haploinsufficient mice,” PLoS Biology, vol.
22. Public Library of Science, 2024.
ista: Burnett L, Koppensteiner P, Symonova O, Masson T, Vega Zuniga TA, Contreras
X, Rülicke T, Shigemoto R, Novarino G, Jösch MA. 2024. Shared behavioural impairments
in visual perception and place avoidance across different autism models are driven
by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice. PLoS
Biology. 22, e3002668.
mla: Burnett, Laura, et al. “Shared Behavioural Impairments in Visual Perception
and Place Avoidance across Different Autism Models Are Driven by Periaqueductal
Grey Hypoexcitability in Setd5 Haploinsufficient Mice.” PLoS Biology, vol.
22, e3002668, Public Library of Science, 2024, doi:10.1371/journal.pbio.3002668.
short: L. Burnett, P. Koppensteiner, O. Symonova, T. Masson, T.A. Vega Zuniga, X.
Contreras, T. Rülicke, R. Shigemoto, G. Novarino, M.A. Jösch, PLoS Biology 22
(2024).
corr_author: '1'
date_created: 2024-06-16T22:01:05Z
date_published: 2024-06-10T00:00:00Z
date_updated: 2025-09-08T07:57:11Z
day: '10'
ddc:
- '570'
department:
- _id: RySh
- _id: GaNo
- _id: MaJö
doi: 10.1371/journal.pbio.3002668
ec_funded: 1
external_id:
isi:
- '001246176800003'
pmid:
- '38857283'
file:
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checksum: 496e1aa4fd5b92b7e4087ecc2c964133
content_type: application/pdf
creator: dernst
date_created: 2025-01-09T10:39:41Z
date_updated: 2025-01-09T10:39:41Z
file_id: '18805'
file_name: 2024_PloS_Burnett.pdf
file_size: 4016568
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success: 1
file_date_updated: 2025-01-09T10:39:41Z
has_accepted_license: '1'
intvolume: ' 22'
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language:
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oa: 1
oa_version: Published Version
pmid: 1
project:
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call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
publication: PLoS Biology
publication_identifier:
eissn:
- 1545-7885
issn:
- 1544-9173
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
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url: https://doi.org/10.5281/zenodo.11130587
record:
- id: '15385'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Shared behavioural impairments in visual perception and place avoidance across
different autism models are driven by periaqueductal grey hypoexcitability in Setd5
haploinsufficient mice
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 22
year: '2024'
...
---
APC_amount: 6828 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18444'
abstract:
- lang: eng
text: Animals rely on compensatory actions to maintain stability and navigate their
environment efficiently. These actions depend on global visual motion cues known
as optic-flow. While the optomotor response has been the traditional focus for
studying optic-flow compensation in insects, its simplicity has been insufficient
to determine the role of the intricate optic-flow processing network involved
in visual course control. Here, we reveal a series of course control behaviours
in Drosophila and link them to specific neural circuits. We show that bilateral
electrical coupling of optic-flow-sensitive neurons in the fly’s lobula plate
are required for a proper course control. This electrical interaction works alongside
chemical synapses within the HS-H2 network to control the dynamics and direction
of turning behaviours. Our findings reveal how insects use bilateral motion cues
for navigation, assigning a new functional significance to the HS-H2 network and
suggesting a previously unknown role for gap junctions in non-linear operations.
acknowledged_ssus:
- _id: Bio
- _id: M-Shop
- _id: LifeSc
acknowledgement: We thank Georg Ammer and Alexander Borst for sharing anti-ShakB serum
antibodies. We thank Nélia Varela and Eugenia Chiappe for the w1118;+;10XUAS-IVS-eGFPKir2.1/TM6B
fly line, Augustin Hrvoje for the shakB[2] line, as well as Jesse Isaacman-Beck
and Thomas R Clandinin for the gift of y1,w*;20XUAS-IVS-PhiC31;+ fly line. We also
thank Armel Nicolas and Tomas Masson for the proteomic analysis, Ece Sönmez for
help with fly crosses and dissections for protein analysis, and Lisa Hofer for assistance
with the reconstruction experiments. We would also like to thank Laura Burnett for
drawing scientific illustrations used in the figures. We are particularly grateful
to members of the Siekhaus, the Kondrashov, and the Chiappe group for providing
material support and technical advice. We are grateful to Daria Siekhaus, Eugenia
Chiappe, Alexander Borst, Ben deBivort, and all the members of the Joesch laboratory
for valuable discussions and comments on the manuscript. Stocks from the Bloomington
Drosophila Stock Center (NIH P40OD018537) and the Vienna Drosophila Resource Center
were used in this study. The Scientific Service Units of ISTA supported the project
through resources provided by the Imaging and Optics Facility, MIBA Machine Shop,
and the Lab Support Facility, as well as Vienna Drosophila Research Centre. This
work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)
as part of the SPP 2205 – 429960716 (M.J.).
article_number: '8830'
article_processing_charge: Yes
article_type: original
author:
- first_name: Victoria
full_name: Pokusaeva, Victoria
id: 3184041C-F248-11E8-B48F-1D18A9856A87
last_name: Pokusaeva
orcid: 0000-0001-7660-444X
- first_name: Roshan K
full_name: Satapathy, Roshan K
id: 46046B7A-F248-11E8-B48F-1D18A9856A87
last_name: Satapathy
orcid: 0009-0006-2974-5075
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Pokusaeva V, Satapathy RK, Symonova O, Jösch MA. Bilateral interactions of
optic-flow sensitive neurons coordinate course control in flies. Nature Communications.
2024;15. doi:10.1038/s41467-024-53173-w
apa: Pokusaeva, V., Satapathy, R. K., Symonova, O., & Jösch, M. A. (2024). Bilateral
interactions of optic-flow sensitive neurons coordinate course control in flies.
Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-024-53173-w
chicago: Pokusaeva, Victoria, Roshan K Satapathy, Olga Symonova, and Maximilian
A Jösch. “Bilateral Interactions of Optic-Flow Sensitive Neurons Coordinate Course
Control in Flies.” Nature Communications. Springer Nature, 2024. https://doi.org/10.1038/s41467-024-53173-w.
ieee: V. Pokusaeva, R. K. Satapathy, O. Symonova, and M. A. Jösch, “Bilateral interactions
of optic-flow sensitive neurons coordinate course control in flies,” Nature
Communications, vol. 15. Springer Nature, 2024.
ista: Pokusaeva V, Satapathy RK, Symonova O, Jösch MA. 2024. Bilateral interactions
of optic-flow sensitive neurons coordinate course control in flies. Nature Communications.
15, 8830.
mla: Pokusaeva, Victoria, et al. “Bilateral Interactions of Optic-Flow Sensitive
Neurons Coordinate Course Control in Flies.” Nature Communications, vol.
15, 8830, Springer Nature, 2024, doi:10.1038/s41467-024-53173-w.
short: V. Pokusaeva, R.K. Satapathy, O. Symonova, M.A. Jösch, Nature Communications
15 (2024).
corr_author: '1'
date_created: 2024-10-20T22:02:05Z
date_published: 2024-10-12T00:00:00Z
date_updated: 2026-06-10T07:58:34Z
day: '12'
ddc:
- '570'
department:
- _id: MaJö
doi: 10.1038/s41467-024-53173-w
external_id:
isi:
- '001336422500001'
pmid:
- '39396050'
file:
- access_level: open_access
checksum: 2af4d6e7364329107aa94d072d594ce0
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creator: dernst
date_created: 2024-10-21T12:11:10Z
date_updated: 2024-10-21T12:11:10Z
file_id: '18459'
file_name: 2024_NatureComm_Pokusaeva.pdf
file_size: 8276667
relation: main_file
success: 1
file_date_updated: 2024-10-21T12:11:10Z
has_accepted_license: '1'
intvolume: ' 15'
isi: 1
language:
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month: '10'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 9B767A34-BA93-11EA-9121-9846C619BF3A
grant_number: '429960716'
name: Evolution of Sensorimotor Transformation Across Diptera
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '18568'
relation: dissertation_contains
status: public
- id: '17488'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Bilateral interactions of optic-flow sensitive neurons coordinate course control
in flies
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2024'
...
---
OA_place: publisher
_id: '18568'
abstract:
- lang: eng
text: "Locomotion is ubiquitous in the animal kingdom because an animal's survival
depends on its ability to navigate its environment to find food, avoid predators
and locate potential mates. These behaviours require control mechanisms that can
extract information from the environment, particularly visual cues. Selective
evolutionary pressures have thus refined such visuomotor transformations in a
species-specific manner to meet the specific ecological and ethological challenges
of each organism. However, a common challenge across organisms as visual information
processing\r\nbecomes increasingly detailed is the mechanisms required to synthesise
disparate pieces of information into a coherent percept or unified picture of
the world. In this thesis, I investigate how disparate visual information is combined
in the brain of Drosophila melanogaster to effectively guide locomotion.\r\nFor
this, I first designed and built a behavioural setup to record locomotion and
present visual stimuli to freely-walking fruit flies in a closed-loop manner.
This setup allowed the investigation of innate visually-guided behaviours, including
the optomotor reflex and courtship.\r\nSecond, taking advantage of my system I
investigated the optomotor response, a reflexive visual stabilisation behaviour
in which flies turn in the direction of global motion to minimise retinal slip.
This behaviour is thought to be mediated by Lobula plate tangential cells (LPTCs);
a complex network of optic-flow-sensitive neurons essential for self-motion estimation.
Using a novel genetic mutant, I demonstrate that electrical coupling between two
LPTC subtypes, contralateral HS and H2 neurons, regulates the balance between
smooth optomotor turning and saccadic anti-optomotor responses. These findings
underscore the critical role of binocular motion cue integration in guiding course
control. Finally, I developed a novel behavioural paradigm in which a sexually
aroused male fruit fly is presented with an optomotor distractor. This setup creates
competition between two visual behaviours, courtship tracking and the optomotor
response, enabling me to explore how the visual system resolves this conflict.
In this setting, males\r\nengaged in courtship selectively suppress their optomotor
response based on the female's location. Furthermore, when this experiment is
replicated with an “artificial female”, optogenetically aroused males alternate
between tracking and optomotor responses. The probability and dynamics of this
switching are determined by the relative strengths of the two competing stimuli.
In summary, the results presented in this thesis explore two mechanisms – integration
and competition - through which visual information is combined in the brain of
the fruit fly to drive locomotion."
acknowledged_ssus:
- _id: M-Shop
acknowledgement: I am incredibly thankful for the outstanding support provided by
ISTA, especially the Machine Shop team, who made conducting research much easier
and more efficient. I am also grateful for the funding provided by European Union’s
Horizon 2020 research and innovation program under the Marie Skłodowska-Curie programme
(665385) and The German Research Foundation grant DFG (SPP2205) “Evolutionary optimization
of neuronal processing”.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Roshan K
full_name: Satapathy, Roshan K
id: 46046B7A-F248-11E8-B48F-1D18A9856A87
last_name: Satapathy
orcid: 0009-0006-2974-5075
citation:
ama: Satapathy RK. Mechanisms of visual integration and competition in innate behaviours
in Drosophila melanogaster. 2024. doi:10.15479/at:ista:18568
apa: Satapathy, R. K. (2024). Mechanisms of visual integration and competition
in innate behaviours in Drosophila melanogaster. Institute of Science and
Technology Austria. https://doi.org/10.15479/at:ista:18568
chicago: Satapathy, Roshan K. “Mechanisms of Visual Integration and Competition
in Innate Behaviours in Drosophila Melanogaster.” Institute of Science and Technology
Austria, 2024. https://doi.org/10.15479/at:ista:18568.
ieee: R. K. Satapathy, “Mechanisms of visual integration and competition in innate
behaviours in Drosophila melanogaster,” Institute of Science and Technology Austria,
2024.
ista: Satapathy RK. 2024. Mechanisms of visual integration and competition in innate
behaviours in Drosophila melanogaster. Institute of Science and Technology Austria.
mla: Satapathy, Roshan K. Mechanisms of Visual Integration and Competition in
Innate Behaviours in Drosophila Melanogaster. Institute of Science and Technology
Austria, 2024, doi:10.15479/at:ista:18568.
short: R.K. Satapathy, Mechanisms of Visual Integration and Competition in Innate
Behaviours in Drosophila Melanogaster, Institute of Science and Technology Austria,
2024.
corr_author: '1'
date_created: 2024-11-19T12:34:30Z
date_published: 2024-11-20T00:00:00Z
date_updated: 2026-04-07T13:00:36Z
day: '20'
ddc:
- '573'
degree_awarded: PhD
department:
- _id: GradSch
- _id: MaJö
doi: 10.15479/at:ista:18568
ec_funded: 1
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date_updated: 2024-11-19T12:39:55Z
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file_name: Roshan PhD thesis-Final.pdf
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language:
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month: '11'
oa: 1
oa_version: Published Version
page: '114'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication_identifier:
isbn:
- 978-3-99078-047-3
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
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relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
title: Mechanisms of visual integration and competition in innate behaviours in Drosophila
melanogaster
tmp:
image: /images/cc_by_sa.png
legal_code_url: https://creativecommons.org/licenses/by-sa/4.0/legalcode
name: Creative Commons Attribution-ShareAlike 4.0 International Public License (CC
BY-SA 4.0)
short: CC BY-SA (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2024'
...
---
OA_place: publisher
_id: '18579'
abstract:
- lang: eng
text: 'Electrophysiological, calcium two-photon recordings and behavioral data for
Vega-Zuniga et al. Relevant information can be found in the ''README.txt'' files. '
acknowledged_ssus:
- _id: ScienComp
- _id: PreCl
- _id: M-Shop
- _id: Bio
- _id: LifeSc
acknowledgement: Freyja Lange, Michael Schunn, and Todor Asenov
article_processing_charge: No
author:
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Anton L
full_name: Sumser, Anton L
id: 3320A096-F248-11E8-B48F-1D18A9856A87
last_name: Sumser
orcid: 0000-0002-4792-1881
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Florian
full_name: Schmidt, Florian
id: A2EF226A-AF19-11E9-924C-0525E6697425
last_name: Schmidt
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA.
A thalamic hub-and-spoke network enables visual perception during action by coordinating
visuomotor dynamics. 2024. doi:10.15479/AT:ISTA:18579
apa: Vega Zuniga, T. A., Sumser, A. L., Symonova, O., Koppensteiner, P., Schmidt,
F., & Jösch, M. A. (2024). A thalamic hub-and-spoke network enables visual
perception during action by coordinating visuomotor dynamics. Institute of Science
and Technology Austria. https://doi.org/10.15479/AT:ISTA:18579
chicago: Vega Zuniga, Tomas A, Anton L Sumser, Olga Symonova, Peter Koppensteiner,
Florian Schmidt, and Maximilian A Jösch. “A Thalamic Hub-and-Spoke Network Enables
Visual Perception during Action by Coordinating Visuomotor Dynamics.” Institute
of Science and Technology Austria, 2024. https://doi.org/10.15479/AT:ISTA:18579.
ieee: T. A. Vega Zuniga, A. L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt,
and M. A. Jösch, “A thalamic hub-and-spoke network enables visual perception during
action by coordinating visuomotor dynamics.” Institute of Science and Technology
Austria, 2024.
ista: Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA.
2024. A thalamic hub-and-spoke network enables visual perception during action
by coordinating visuomotor dynamics, Institute of Science and Technology Austria,
10.15479/AT:ISTA:18579.
mla: Vega Zuniga, Tomas A., et al. A Thalamic Hub-and-Spoke Network Enables Visual
Perception during Action by Coordinating Visuomotor Dynamics. Institute of
Science and Technology Austria, 2024, doi:10.15479/AT:ISTA:18579.
short: T.A. Vega Zuniga, A.L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt,
M.A. Jösch, (2024).
corr_author: '1'
date_created: 2024-11-22T13:48:12Z
date_published: 2024-12-09T00:00:00Z
date_updated: 2026-06-18T18:12:08Z
day: '09'
ddc:
- '570'
department:
- _id: MaJö
doi: 10.15479/AT:ISTA:18579
ec_funded: 1
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date_updated: 2024-12-09T10:24:25Z
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date_updated: 2024-12-09T10:21:10Z
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date_created: 2024-12-09T12:54:55Z
date_updated: 2024-12-09T12:54:55Z
file_id: '18637'
file_name: readme.txt
file_size: 505
relation: main_file
success: 1
file_date_updated: 2024-12-09T12:54:55Z
has_accepted_license: '1'
month: '12'
oa: 1
oa_version: Published Version
project:
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1098-2017
name: Connecting sensory with motor processing in the superior colliculus
- _id: 266D407A-B435-11E9-9278-68D0E5697425
grant_number: LT000256
name: Neuronal networks of salience and spatial detection in the murine superior
colliculus
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
- _id: bdaf81a8-d553-11ed-ba76-c95961984540
grant_number: '101086580'
name: 'Action Selection in the Midbrain: Neuromodulation of Visuomotor Senses'
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '19076'
relation: used_in_publication
status: public
status: public
title: A thalamic hub-and-spoke network enables visual perception during action by
coordinating visuomotor dynamics
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '17488'
abstract:
- lang: eng
text: Behavioural data for Pokusaeva, Satapathy et al. Relevant information can
be found in the 'README.txt' file.
acknowledged_ssus:
- _id: M-Shop
article_processing_charge: No
author:
- first_name: Roshan K
full_name: Satapathy, Roshan K
id: 46046B7A-F248-11E8-B48F-1D18A9856A87
last_name: Satapathy
orcid: 0009-0006-2974-5075
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Victoria
full_name: Pokusaeva, Victoria
id: 3184041C-F248-11E8-B48F-1D18A9856A87
last_name: Pokusaeva
orcid: 0000-0001-7660-444X
citation:
ama: Satapathy RK, Jösch MA, Symonova O, Pokusaeva V. Bilateral interactions of
optic-flow sensitive neurons coordinate course control in flies. 2024. doi:10.15479/AT:ISTA:17488
apa: Satapathy, R. K., Jösch, M. A., Symonova, O., & Pokusaeva, V. (2024). Bilateral
interactions of optic-flow sensitive neurons coordinate course control in flies.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:17488
chicago: Satapathy, Roshan K, Maximilian A Jösch, Olga Symonova, and Victoria Pokusaeva.
“Bilateral Interactions of Optic-Flow Sensitive Neurons Coordinate Course Control
in Flies.” Institute of Science and Technology Austria, 2024. https://doi.org/10.15479/AT:ISTA:17488.
ieee: R. K. Satapathy, M. A. Jösch, O. Symonova, and V. Pokusaeva, “Bilateral interactions
of optic-flow sensitive neurons coordinate course control in flies.” Institute
of Science and Technology Austria, 2024.
ista: Satapathy RK, Jösch MA, Symonova O, Pokusaeva V. 2024. Bilateral interactions
of optic-flow sensitive neurons coordinate course control in flies, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:17488.
mla: Satapathy, Roshan K., et al. Bilateral Interactions of Optic-Flow Sensitive
Neurons Coordinate Course Control in Flies. Institute of Science and Technology
Austria, 2024, doi:10.15479/AT:ISTA:17488.
short: R.K. Satapathy, M.A. Jösch, O. Symonova, V. Pokusaeva, (2024).
corr_author: '1'
date_created: 2024-09-03T17:42:46Z
date_published: 2024-09-01T00:00:00Z
date_updated: 2026-06-10T07:58:35Z
ddc:
- '570'
department:
- _id: GradSch
- _id: MaJö
doi: 10.15479/AT:ISTA:17488
file:
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checksum: df9d6c8ddffa046c3b1639281f83cfcf
content_type: application/x-zip-compressed
creator: rsatapat
date_created: 2024-09-03T17:39:32Z
date_updated: 2024-09-03T17:39:32Z
file_id: '17489'
file_name: BehaviouralData.zip
file_size: 965778072
relation: main_file
success: 1
file_date_updated: 2024-09-03T17:39:32Z
has_accepted_license: '1'
keyword:
- drosophila
- behaviour
- locomotion
- gap junctions
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 9B767A34-BA93-11EA-9121-9846C619BF3A
grant_number: '429960716'
name: Evolution of Sensorimotor Transformation Across Diptera
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '18444'
relation: used_in_publication
status: public
status: public
title: Bilateral interactions of optic-flow sensitive neurons coordinate course control
in flies
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d
year: '2024'
...
---
OA_embargo: '12'
OA_place: publisher
_id: '18574'
abstract:
- lang: eng
text: "Biological vision is unlike a camera; rather than transmitting light information
faithfully, early\r\nvisual circuits process the visual scene to convey only the
relevant information in an efficient\r\nmanner. Consequentially, the nature of
this visual processing then depends on what is the\r\nrelevant information in
a scene and on the notion of efficiency. In this work, I study how visual\r\nprocessing
is modulated by two different variations in the visual scene. First, I discovered
that\r\nin the mouse (Mus musculus) retina, Retinal Ganglion Cells in the upper
and lower visual\r\nfield have differences in the center surround structure of
their receptive fields. Comparison\r\nwith models of efficient coding show that
this adaptation likely evolved to cope with the\r\nbrightness gradient from the
sky to the ground that is pervasive in natural scenes. In the\r\nsecond project,
I study how the downstream neurons in the Superior Colliculus dynamically\r\nchange
their temporal selectivity depending on the ambient luminance and behavioral state.\r\nAs
the scene gets darker or when the animal is is less aroused, the neuronal responses
get\r\nlaggier, while still maintaining their relative timing with respect to
the population. Overall, this\r\nwork emphasises the need to understand visual
processing in the context of specific demands\r\nof the animal in its the environment.
The adaptive changes in the visual system, from the\r\nretinal ganglion cells
to the superior colliculus, highlight the intricate ways in which biological\r\nvision
optimizes the processing of visual information.\r\n"
acknowledged_ssus:
- _id: Bio
- _id: ScienComp
- _id: PreCl
- _id: LifeSc
- _id: M-Shop
- _id: E-Lib
acknowledgement: "This work would have been impossible without the Scientific Service
Units of IST Austria. The resources and expertise provided by Scientific Computing
(especially Alois Schlögl), the MIBA Machine Shop (especially Todor Asenov), the
Preclinical Facility (especially Freyja Langer), the Library, the Lab Support Facility
and the Imaging and Optics Facility were the essential bedrock I could build upon.
I would also like to thank IT support at ISTA for powering through remote work and
a cyberattack.\r\nI am grateful for having been funded initially by the European
Union Horizon 2020 Marie Skłodowska-Curie grant 665385 and later by Prof. Maximilian
Joesch's the European Research Council Starting (756502) and Consolidator (101086580)
Grants."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Divyansh
full_name: Gupta, Divyansh
id: 2A485EBE-F248-11E8-B48F-1D18A9856A87
last_name: Gupta
orcid: 0000-0001-7400-6665
citation:
ama: Gupta D. Visual adaptations to natural statistics. 2024. doi:10.15479/at:ista:18574
apa: Gupta, D. (2024). Visual adaptations to natural statistics. Institute
of Science and Technology Austria. https://doi.org/10.15479/at:ista:18574
chicago: Gupta, Divyansh. “Visual Adaptations to Natural Statistics.” Institute
of Science and Technology Austria, 2024. https://doi.org/10.15479/at:ista:18574.
ieee: D. Gupta, “Visual adaptations to natural statistics,” Institute of Science
and Technology Austria, 2024.
ista: Gupta D. 2024. Visual adaptations to natural statistics. Institute of Science
and Technology Austria.
mla: Gupta, Divyansh. Visual Adaptations to Natural Statistics. Institute
of Science and Technology Austria, 2024, doi:10.15479/at:ista:18574.
short: D. Gupta, Visual Adaptations to Natural Statistics, Institute of Science
and Technology Austria, 2024.
corr_author: '1'
date_created: 2024-11-20T21:30:44Z
date_published: 2024-11-22T00:00:00Z
date_updated: 2026-04-07T13:24:48Z
day: '22'
ddc:
- '573'
degree_awarded: PhD
department:
- _id: GradSch
- _id: MaJö
doi: 10.15479/at:ista:18574
ec_funded: 1
file:
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creator: dgupta
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date_updated: 2025-11-11T23:30:02Z
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file_size: 75512262
relation: source_file
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checksum: 1282401eb71598bc311058b0fcefc6a1
content_type: application/pdf
creator: dgupta
date_created: 2024-11-26T11:43:19Z
date_updated: 2025-11-11T23:30:02Z
embargo: 2025-11-11
file_id: '18591'
file_name: PDFA_PhD_Thesis___Divyansh_Gupta-26_11_24.pdf
file_size: 6412619
relation: main_file
file_date_updated: 2025-11-11T23:30:02Z
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
month: '11'
oa: 1
oa_version: Published Version
page: '86'
project:
- _id: bdaf81a8-d553-11ed-ba76-c95961984540
grant_number: '101086580'
name: 'Action Selection in the Midbrain: Neuromodulation of Visuomotor Senses'
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
publication_identifier:
isbn:
- 978-3-99078-050-3
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '12349'
relation: part_of_dissertation
status: public
- id: '12370'
relation: research_data
status: public
status: public
supervisor:
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
title: Visual adaptations to natural statistics
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2024'
...
---
OA_place: publisher
_id: '12716'
abstract:
- lang: eng
text: "The process of detecting and evaluating sensory information to guide behaviour
is termed perceptual decision-making (PDM), and is critical for the ability of
an organism to interact with its external world. Individuals with autism, a neurodevelopmental
condition primarily characterised by social and communication difficulties, frequently
exhibit altered sensory processing and PDM difficulties are widely reported. Recent
technological advancements have pushed forward our understanding of the genetic
changes accompanying this condition, however our understanding of how these mutations
affect the function of specific neuronal circuits and bring about the corresponding
behavioural changes remains limited. Here, we use an innate PDM task, the looming
avoidance response (LAR) paradigm, to identify a convergent behavioural abnormality
across three molecularly distinct genetic mouse models of autism (Cul3, Setd5
and Ptchd1). Although mutant mice can rapidly detect threatening visual stimuli,
their responses are consistently delayed, requiring longer to initiate an appropriate
response than their wild-type siblings. Mutant animals show abnormal adaptation
in both their stimulus- evoked escape responses and exploratory dynamics following
repeated stimulus presentations. Similarly delayed behavioural responses are observed
in wild-type animals when faced with more ambiguous threats, suggesting the mutant
phenotype could arise from a dysfunction in the flexible control of this PDM process.\r\nOur
knowledge of the core neuronal circuitry mediating the LAR facilitated a detailed
dissection of the neuronal mechanisms underlying the behavioural impairment. In
vivo extracellular recording revealed that visual responses were unaffected within
a key brain region for the rapid processing of visual threats, the superior colliculus
(SC), indicating that the behavioural delay was unlikely to originate from sensory
impairments. Delayed behavioural responses were recapitulated in the Setd5 model
following optogenetic stimulation of the excitatory output neurons of the SC,
which are known to mediate escape initiation through the activation of cells in
the underlying dorsal periaqueductal grey (dPAG). In vitro patch-clamp recordings
of dPAG cells uncovered a stark hypoexcitability phenotype in two out of the three
genetic models investigated (Setd5 and Ptchd1), that in Setd5, is mediated by
the misregulation of voltage-gated potassium channels. Overall, our results show
that the ability to use visual information to drive efficient escape responses
is impaired in three diverse genetic mouse models of autism and that, in one of
the models studied, this behavioural delay likely originates from differences
in the intrinsic excitability of a key subcortical node, the dPAG. Furthermore,
this work showcases the use of an innate behavioural paradigm to mechanistically
dissect PDM processes in autism."
acknowledged_ssus:
- _id: PreCl
- _id: Bio
- _id: LifeSc
- _id: M-Shop
- _id: CampIT
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Laura
full_name: Burnett, Laura
id: 3B717F68-F248-11E8-B48F-1D18A9856A87
last_name: Burnett
orcid: 0000-0002-8937-410X
citation:
ama: Burnett L. To flee, or not to flee? Using innate defensive behaviours to investigate
rapid perceptual decision-making through subcortical circuits in mouse models
of autism. 2023. doi:10.15479/at:ista:12716
apa: Burnett, L. (2023). To flee, or not to flee? Using innate defensive behaviours
to investigate rapid perceptual decision-making through subcortical circuits in
mouse models of autism. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12716
chicago: Burnett, Laura. “To Flee, or Not to Flee? Using Innate Defensive Behaviours
to Investigate Rapid Perceptual Decision-Making through Subcortical Circuits in
Mouse Models of Autism.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12716.
ieee: L. Burnett, “To flee, or not to flee? Using innate defensive behaviours to
investigate rapid perceptual decision-making through subcortical circuits in mouse
models of autism,” Institute of Science and Technology Austria, 2023.
ista: Burnett L. 2023. To flee, or not to flee? Using innate defensive behaviours
to investigate rapid perceptual decision-making through subcortical circuits in
mouse models of autism. Institute of Science and Technology Austria.
mla: Burnett, Laura. To Flee, or Not to Flee? Using Innate Defensive Behaviours
to Investigate Rapid Perceptual Decision-Making through Subcortical Circuits in
Mouse Models of Autism. Institute of Science and Technology Austria, 2023,
doi:10.15479/at:ista:12716.
short: L. Burnett, To Flee, or Not to Flee? Using Innate Defensive Behaviours to
Investigate Rapid Perceptual Decision-Making through Subcortical Circuits in Mouse
Models of Autism, Institute of Science and Technology Austria, 2023.
corr_author: '1'
date_created: 2023-03-08T15:19:45Z
date_published: 2023-03-10T00:00:00Z
date_updated: 2026-04-07T13:25:15Z
day: '10'
ddc:
- '599'
- '573'
degree_awarded: PhD
department:
- _id: GradSch
- _id: MaJö
doi: 10.15479/at:ista:12716
ec_funded: 1
file:
- access_level: closed
checksum: 6c6d9cc2c4cdacb74e6b1047a34d7332
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: lburnett
date_created: 2023-03-08T15:08:46Z
date_updated: 2023-03-08T15:08:46Z
file_id: '12717'
file_name: Burnett_Thesis_2023.docx
file_size: 23029260
relation: source_file
- access_level: open_access
checksum: cebc77705288bf4382db9b3541483cd0
content_type: application/pdf
creator: lburnett
date_created: 2023-03-08T15:08:46Z
date_updated: 2023-03-08T15:08:46Z
file_id: '12718'
file_name: Burnett_Thesis_2023_pdfA.pdf
file_size: 11959869
relation: main_file
success: 1
file_date_updated: 2023-03-08T15:08:46Z
has_accepted_license: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: '178'
project:
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
title: To flee, or not to flee? Using innate defensive behaviours to investigate rapid
perceptual decision-making through subcortical circuits in mouse models of autism
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2023'
...
---
OA_place: publisher
_id: '12826'
abstract:
- lang: eng
text: "During navigation, animals can infer the structure of the environment by
computing the optic flow cues elicited by their own movements, and subsequently
use this information to instruct proper locomotor actions. These computations
require a panoramic assessment of the visual environment in order to disambiguate
similar sensory experiences that may require distinct behavioral responses. The
estimation of the global motion patterns is therefore essential for successful
navigation. Yet, our understanding of the algorithms and implementations that
enable coherent panoramic visual perception remains scarce. Here I pursue this
problem by dissecting the functional aspects of interneuronal communication in
the lobula plate tangential cell network in Drosophila melanogaster. The results
presented in the thesis demonstrate that the basis for effective interpretation
of the optic flow in this circuit are stereotyped synaptic connections that mediate
the formation of distinct subnetworks, each extracting a particular pattern of
global motion. \r\nFirstly, I show that gap junctions are essential for a correct
interpretation of binocular motion cues by horizontal motion-sensitive cells.
HS cells form electrical synapses with contralateral H2 neurons that are involved
in detecting yaw rotation and translation. I developed an FlpStop-mediated mutant
of a gap junction protein ShakB that disrupts these electrical synapses. While
the loss of electrical synapses does not affect the tuning of the direction selectivity
in HS neurons, it severely alters their sensitivity to horizontal motion in the
contralateral side. These physiological changes result in an inappropriate integration
of binocular motion cues in walking animals. While wild-type flies form a binocular
perception of visual motion by non-linear integration of monocular optic flow
cues, the mutant flies sum the monocular inputs linearly. These results indicate
that rather than averaging signals in neighboring neurons, gap-junctions operate
in conjunction with chemical synapses to mediate complex non-linear optic flow
computations.\r\nSecondly, I show that stochastic manipulation of neuronal activity
in the lobula plate tangential cell network is a powerful approach to study the
neuronal implementation of optic flow-based navigation in flies. Tangential neurons
form multiple subnetworks, each mediating course-stabilizing response to a particular
global pattern of visual motion. Application of genetic mosaic techniques can
provide sparse optogenetic activation of HS cells in numerous combinations. These
distinct combinations of activated neurons drive an array of distinct behavioral
responses, providing important insights into how visuomotor transformation is
performed in the lobula plate tangential cell network. This approach can be complemented
by stochastic silencing of tangential neurons, enabling direct assessment of the
functional role of individual tangential neurons in the processing of specific
visual motion patterns.\r\n\tTaken together, the findings presented in this thesis
suggest that establishing specific activity patterns of tangential cells via stereotyped
synaptic connectivity is a key to efficient optic flow-based navigation in Drosophila
melanogaster."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Victoria
full_name: Pokusaeva, Victoria
id: 3184041C-F248-11E8-B48F-1D18A9856A87
last_name: Pokusaeva
orcid: 0000-0001-7660-444X
citation:
ama: Pokusaeva V. Neural control of optic flow-based navigation in Drosophila melanogaster.
2023. doi:10.15479/at:ista:12826
apa: Pokusaeva, V. (2023). Neural control of optic flow-based navigation in Drosophila
melanogaster. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12826
chicago: Pokusaeva, Victoria. “Neural Control of Optic Flow-Based Navigation in
Drosophila Melanogaster.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12826.
ieee: V. Pokusaeva, “Neural control of optic flow-based navigation in Drosophila
melanogaster,” Institute of Science and Technology Austria, 2023.
ista: Pokusaeva V. 2023. Neural control of optic flow-based navigation in Drosophila
melanogaster. Institute of Science and Technology Austria.
mla: Pokusaeva, Victoria. Neural Control of Optic Flow-Based Navigation in Drosophila
Melanogaster. Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:12826.
short: V. Pokusaeva, Neural Control of Optic Flow-Based Navigation in Drosophila
Melanogaster, Institute of Science and Technology Austria, 2023.
corr_author: '1'
date_created: 2023-04-14T14:56:04Z
date_published: 2023-04-18T00:00:00Z
date_updated: 2026-04-07T13:26:49Z
day: '18'
ddc:
- '570'
- '571'
degree_awarded: PhD
department:
- _id: MaJö
- _id: GradSch
doi: 10.15479/at:ista:12826
ec_funded: 1
file:
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file_id: '12857'
file_name: Thesis_Pokusaeva.docx
file_size: 14507243
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content_type: application/pdf
creator: vpokusae
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date_updated: 2023-04-20T09:14:44Z
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has_accepted_license: '1'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: '106'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
title: Neural control of optic flow-based navigation in Drosophila melanogaster
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2023'
...
---
_id: '13230'
abstract:
- lang: eng
text: 'To interpret the sensory environment, the brain combines ambiguous sensory
measurements with knowledge that reflects context-specific prior experience. But
environmental contexts can change abruptly and unpredictably, resulting in uncertainty
about the current context. Here we address two questions: how should context-specific
prior knowledge optimally guide the interpretation of sensory stimuli in changing
environments, and do human decision-making strategies resemble this optimum? We
probe these questions with a task in which subjects report the orientation of
ambiguous visual stimuli that were drawn from three dynamically switching distributions,
representing different environmental contexts. We derive predictions for an ideal
Bayesian observer that leverages knowledge about the statistical structure of
the task to maximize decision accuracy, including knowledge about the dynamics
of the environment. We show that its decisions are biased by the dynamically changing
task context. The magnitude of this decision bias depends on the observer’s continually
evolving belief about the current context. The model therefore not only predicts
that decision bias will grow as the context is indicated more reliably, but also
as the stability of the environment increases, and as the number of trials since
the last context switch grows. Analysis of human choice data validates all three
predictions, suggesting that the brain leverages knowledge of the statistical
structure of environmental change when interpreting ambiguous sensory signals.'
acknowledgement: The authors thank Corey Ziemba and Zoe Boundy-Singer for valuable
discussion and feedback.
article_number: e1011104
article_processing_charge: No
article_type: original
author:
- first_name: Julie A.
full_name: Charlton, Julie A.
last_name: Charlton
- first_name: Wiktor F
full_name: Mlynarski, Wiktor F
id: 358A453A-F248-11E8-B48F-1D18A9856A87
last_name: Mlynarski
- first_name: Yoon H.
full_name: Bai, Yoon H.
last_name: Bai
- first_name: Ann M.
full_name: Hermundstad, Ann M.
last_name: Hermundstad
- first_name: Robbe L.T.
full_name: Goris, Robbe L.T.
last_name: Goris
citation:
ama: Charlton JA, Mlynarski WF, Bai YH, Hermundstad AM, Goris RLT. Environmental
dynamics shape perceptual decision bias. PLoS Computational Biology. 2023;19(6).
doi:10.1371/journal.pcbi.1011104
apa: Charlton, J. A., Mlynarski, W. F., Bai, Y. H., Hermundstad, A. M., & Goris,
R. L. T. (2023). Environmental dynamics shape perceptual decision bias. PLoS
Computational Biology. Public Library of Science. https://doi.org/10.1371/journal.pcbi.1011104
chicago: Charlton, Julie A., Wiktor F Mlynarski, Yoon H. Bai, Ann M. Hermundstad,
and Robbe L.T. Goris. “Environmental Dynamics Shape Perceptual Decision Bias.”
PLoS Computational Biology. Public Library of Science, 2023. https://doi.org/10.1371/journal.pcbi.1011104.
ieee: J. A. Charlton, W. F. Mlynarski, Y. H. Bai, A. M. Hermundstad, and R. L. T.
Goris, “Environmental dynamics shape perceptual decision bias,” PLoS Computational
Biology, vol. 19, no. 6. Public Library of Science, 2023.
ista: Charlton JA, Mlynarski WF, Bai YH, Hermundstad AM, Goris RLT. 2023. Environmental
dynamics shape perceptual decision bias. PLoS Computational Biology. 19(6), e1011104.
mla: Charlton, Julie A., et al. “Environmental Dynamics Shape Perceptual Decision
Bias.” PLoS Computational Biology, vol. 19, no. 6, e1011104, Public Library
of Science, 2023, doi:10.1371/journal.pcbi.1011104.
short: J.A. Charlton, W.F. Mlynarski, Y.H. Bai, A.M. Hermundstad, R.L.T. Goris,
PLoS Computational Biology 19 (2023).
date_created: 2023-07-16T22:01:09Z
date_published: 2023-06-08T00:00:00Z
date_updated: 2023-08-02T06:33:50Z
day: '08'
ddc:
- '570'
department:
- _id: MaJö
doi: 10.1371/journal.pcbi.1011104
external_id:
isi:
- '001003410200003'
pmid:
- '37289753'
file:
- access_level: open_access
checksum: 800761fa2c647fabd6ad034589bc526e
content_type: application/pdf
creator: dernst
date_created: 2023-07-18T08:07:59Z
date_updated: 2023-07-18T08:07:59Z
file_id: '13247'
file_name: 2023_PloSCompBio_Charlton.pdf
file_size: 2281868
relation: main_file
success: 1
file_date_updated: 2023-07-18T08:07:59Z
has_accepted_license: '1'
intvolume: ' 19'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS Computational Biology
publication_identifier:
eissn:
- 1553-7358
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Environmental dynamics shape perceptual decision bias
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 19
year: '2023'
...
---
OA_place: publisher
_id: '12531'
abstract:
- lang: eng
text: "All visual experiences of the vertebrates begin with light being converted
into electrical signals\r\nby the eye retina. Retinal ganglion cells (RGCs) are
the neurons of the innermost layer of the\r\nmammal retina, and they transmit
visual information to the rest of the brain.\r\nIt has been shown that RGCs vary
in their morphology and genetic profiles, moreover they can\r\nbe unambiguously
grouped into subtypes that share the same morphological and/or molecular\r\nproperties.
However, in terms of RGCs function, it remains unclear how many distinct types\r\nthere
are and what response properties their typology relies on. Even given the recent
studies\r\nthat successfully classified RGCs in a patch of the retina [1] and
in scotopic conditions [2], the\r\nquestion remains whether the found subtypes
persist across the entire retina.\r\nIn this work, using a novel imaging method,
we show that, when sampled from a large portion\r\nof the retina, RGCs can not
be clearly divided into functional subtypes. We found that in\r\nphotopic conditions,
which implies more prominent natural scene statistic differences across\r\nthe
visual field, response properties can be exhibited by cells differently depending
on their\r\nlocation in the retina, which leads to formation of a gradient of
features rather than distinct\r\nclasses.\r\nThis finding suggests that RGCs follow
a global organization across the visual field of the\r\nanimal, adapting each
RGC subtype to the requirements imposed by the natural scene statistics."
alternative_title:
- ISTA Master's Thesis
article_processing_charge: No
author:
- first_name: Kseniia
full_name: Kirillova, Kseniia
id: 8e3f931e-dc85-11ea-9058-e7b957bf23f0
last_name: Kirillova
citation:
ama: Kirillova K. Panoramic functional gradients across the mouse retina. 2023.
doi:10.15479/at:ista:12531
apa: Kirillova, K. (2023). Panoramic functional gradients across the mouse retina.
Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12531
chicago: Kirillova, Kseniia. “Panoramic Functional Gradients across the Mouse Retina.”
Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12531.
ieee: K. Kirillova, “Panoramic functional gradients across the mouse retina,” Institute
of Science and Technology Austria, 2023.
ista: Kirillova K. 2023. Panoramic functional gradients across the mouse retina.
Institute of Science and Technology Austria.
mla: Kirillova, Kseniia. Panoramic Functional Gradients across the Mouse Retina.
Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:12531.
short: K. Kirillova, Panoramic Functional Gradients across the Mouse Retina, Institute
of Science and Technology Austria, 2023.
corr_author: '1'
date_created: 2023-02-09T07:45:05Z
date_published: 2023-02-08T00:00:00Z
date_updated: 2026-04-07T14:06:26Z
day: '08'
ddc:
- '570'
degree_awarded: MS
department:
- _id: GradSch
- _id: MaJö
doi: 10.15479/at:ista:12531
file:
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creator: cchlebak
date_created: 2023-02-10T09:32:06Z
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file_id: '12535'
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file_date_updated: 2024-02-09T23:30:03Z
has_accepted_license: '1'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: '46'
publication_identifier:
issn:
- 2791-4585
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
title: Panoramic functional gradients across the mouse retina
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2023'
...
---
_id: '12349'
abstract:
- lang: eng
text: Statistics of natural scenes are not uniform - their structure varies dramatically
from ground to sky. It remains unknown whether these non-uniformities are reflected
in the large-scale organization of the early visual system and what benefits such
adaptations would confer. Here, by relying on the efficient coding hypothesis,
we predict that changes in the structure of receptive fields across visual space
increase the efficiency of sensory coding. We show experimentally that, in agreement
with our predictions, receptive fields of retinal ganglion cells change their
shape along the dorsoventral retinal axis, with a marked surround asymmetry at
the visual horizon. Our work demonstrates that, according to principles of efficient
coding, the panoramic structure of natural scenes is exploited by the retina across
space and cell-types.
acknowledged_ssus:
- _id: ScienComp
- _id: PreCl
- _id: LifeSc
- _id: Bio
acknowledgement: We thank Hiroki Asari for sharing the dataset of naturalistic images,
Anton Sumser for sharing visual stimulus code, Yoav Ben Simon for initial explorative
work with the generation of AAVs, and Tomas Vega-Zuñiga for help with immunostainings.
We also thank Gasper Tkacik and members of the Neuroethology group for their comments
on the manuscript. This research was supported by the Scientific Service Units of
IST Austria through resources provided by Scientific Computing, the Preclinical
Facility, the Lab Support Facility, and the Imaging and Optics Facility. This work
was supported by European Union Horizon 2020 Marie Skłodowska-Curie grant 665385
(DG), Austrian Science Fund (FWF) stand-alone grant P 34015 (WM), Human Frontiers
Science Program LT000256/2018-L (AS), EMBO ALTF 1098-2017 (AS) and the European
Research Council Starting Grant 756502 (MJ).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Divyansh
full_name: Gupta, Divyansh
id: 2A485EBE-F248-11E8-B48F-1D18A9856A87
last_name: Gupta
orcid: 0000-0001-7400-6665
- first_name: Wiktor F
full_name: Mlynarski, Wiktor F
id: 358A453A-F248-11E8-B48F-1D18A9856A87
last_name: Mlynarski
- first_name: Anton L
full_name: Sumser, Anton L
id: 3320A096-F248-11E8-B48F-1D18A9856A87
last_name: Sumser
orcid: 0000-0002-4792-1881
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Jan
full_name: Svaton, Jan
id: f7f724c3-9d6f-11ed-9f44-e5c5f3a5bee2
last_name: Svaton
orcid: 0000-0002-6198-2939
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Gupta D, Mlynarski WF, Sumser AL, Symonova O, Svaton J, Jösch MA. Panoramic
visual statistics shape retina-wide organization of receptive fields. Nature
Neuroscience. 2023;26:606-614. doi:10.1038/s41593-023-01280-0
apa: Gupta, D., Mlynarski, W. F., Sumser, A. L., Symonova, O., Svaton, J., &
Jösch, M. A. (2023). Panoramic visual statistics shape retina-wide organization
of receptive fields. Nature Neuroscience. Springer Nature. https://doi.org/10.1038/s41593-023-01280-0
chicago: Gupta, Divyansh, Wiktor F Mlynarski, Anton L Sumser, Olga Symonova, Jan
Svaton, and Maximilian A Jösch. “Panoramic Visual Statistics Shape Retina-Wide
Organization of Receptive Fields.” Nature Neuroscience. Springer Nature,
2023. https://doi.org/10.1038/s41593-023-01280-0.
ieee: D. Gupta, W. F. Mlynarski, A. L. Sumser, O. Symonova, J. Svaton, and M. A.
Jösch, “Panoramic visual statistics shape retina-wide organization of receptive
fields,” Nature Neuroscience, vol. 26. Springer Nature, pp. 606–614, 2023.
ista: Gupta D, Mlynarski WF, Sumser AL, Symonova O, Svaton J, Jösch MA. 2023. Panoramic
visual statistics shape retina-wide organization of receptive fields. Nature Neuroscience.
26, 606–614.
mla: Gupta, Divyansh, et al. “Panoramic Visual Statistics Shape Retina-Wide Organization
of Receptive Fields.” Nature Neuroscience, vol. 26, Springer Nature, 2023,
pp. 606–14, doi:10.1038/s41593-023-01280-0.
short: D. Gupta, W.F. Mlynarski, A.L. Sumser, O. Symonova, J. Svaton, M.A. Jösch,
Nature Neuroscience 26 (2023) 606–614.
corr_author: '1'
date_created: 2023-01-23T14:14:19Z
date_published: 2023-04-01T00:00:00Z
date_updated: 2026-07-02T22:30:22Z
day: '01'
ddc:
- '570'
department:
- _id: GradSch
- _id: MaJö
doi: 10.1038/s41593-023-01280-0
ec_funded: 1
external_id:
isi:
- '000955258300002'
pmid:
- '36959418'
file:
- access_level: open_access
checksum: a33d91e398e548f34003170e10988368
content_type: application/pdf
creator: dernst
date_created: 2023-10-04T11:40:51Z
date_updated: 2023-10-04T11:40:51Z
file_id: '14395'
file_name: 2023_NatureNeuroscience_Gupta.pdf
file_size: 6144866
relation: main_file
success: 1
file_date_updated: 2023-10-04T11:40:51Z
has_accepted_license: '1'
intvolume: ' 26'
isi: 1
language:
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month: '04'
oa: 1
oa_version: Published Version
page: 606-614
pmid: 1
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 626c45b5-2b32-11ec-9570-e509828c1ba6
grant_number: P34015
name: Efficient coding with biophysical realism
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
- _id: 266D407A-B435-11E9-9278-68D0E5697425
grant_number: LT000256
name: Neuronal networks of salience and spatial detection in the murine superior
colliculus
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1098-2017
name: Connecting sensory with motor processing in the superior colliculus
publication: Nature Neuroscience
publication_identifier:
eissn:
- 1546-1726
issn:
- 1097-6256
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '12370'
relation: research_data
status: public
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relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Panoramic visual statistics shape retina-wide organization of receptive fields
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 26
year: '2023'
...
---
_id: '12370'
abstract:
- lang: eng
text: 'Statistics of natural scenes are not uniform - their structure varies dramatically
from ground to sky. It remains unknown whether these non-uniformities are reflected
in the large-scale organization of the early visual system and what benefits such
adaptations would confer. Here, by relying on the efficient coding hypothesis,
we predict that changes in the structure of receptive fields across visual space
increase the efficiency of sensory coding. We show experimentally that, in agreement
with our predictions, receptive fields of retinal ganglion cells change their
shape along the dorsoventral retinal axis, with a marked surround asymmetry at
the visual horizon. Our work demonstrates that, according to principles of efficient
coding, the panoramic structure of natural scenes is exploited by the retina across
space and cell-types. '
acknowledged_ssus:
- _id: ScienComp
- _id: M-Shop
- _id: Bio
- _id: PreCl
- _id: LifeSc
article_processing_charge: No
author:
- first_name: Divyansh
full_name: Gupta, Divyansh
id: 2A485EBE-F248-11E8-B48F-1D18A9856A87
last_name: Gupta
orcid: 0000-0001-7400-6665
- first_name: Anton L
full_name: Sumser, Anton L
id: 3320A096-F248-11E8-B48F-1D18A9856A87
last_name: Sumser
orcid: 0000-0002-4792-1881
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: 'Gupta D, Sumser AL, Jösch MA. Research Data for: Panoramic visual statistics
shape retina-wide organization of receptive fields. 2023. doi:10.15479/AT:ISTA:12370'
apa: 'Gupta, D., Sumser, A. L., & Jösch, M. A. (2023). Research Data for: Panoramic
visual statistics shape retina-wide organization of receptive fields. Institute
of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:12370'
chicago: 'Gupta, Divyansh, Anton L Sumser, and Maximilian A Jösch. “Research Data
for: Panoramic Visual Statistics Shape Retina-Wide Organization of Receptive Fields.”
Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/AT:ISTA:12370.'
ieee: 'D. Gupta, A. L. Sumser, and M. A. Jösch, “Research Data for: Panoramic visual
statistics shape retina-wide organization of receptive fields.” Institute of Science
and Technology Austria, 2023.'
ista: 'Gupta D, Sumser AL, Jösch MA. 2023. Research Data for: Panoramic visual statistics
shape retina-wide organization of receptive fields, Institute of Science and Technology
Austria, 10.15479/AT:ISTA:12370.'
mla: 'Gupta, Divyansh, et al. Research Data for: Panoramic Visual Statistics
Shape Retina-Wide Organization of Receptive Fields. Institute of Science and
Technology Austria, 2023, doi:10.15479/AT:ISTA:12370.'
short: D. Gupta, A.L. Sumser, M.A. Jösch, (2023).
contributor:
- contributor_type: researcher
first_name: Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
- contributor_type: researcher
first_name: Wiktor F
id: 358A453A-F248-11E8-B48F-1D18A9856A87
last_name: Mlynarski
- contributor_type: researcher
first_name: Jan
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last_name: Svaton
corr_author: '1'
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date_published: 2023-01-26T00:00:00Z
date_updated: 2026-07-02T22:30:22Z
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ddc:
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department:
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- _id: MaJö
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ec_funded: 1
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has_accepted_license: '1'
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 626c45b5-2b32-11ec-9570-e509828c1ba6
grant_number: P34015
name: Efficient coding with biophysical realism
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
- _id: 266D407A-B435-11E9-9278-68D0E5697425
grant_number: LT000256
name: Neuronal networks of salience and spatial detection in the murine superior
colliculus
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1098-2017
name: Connecting sensory with motor processing in the superior colliculus
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '12349'
relation: used_in_publication
status: public
- id: '18574'
relation: used_in_publication
status: public
status: public
title: 'Research Data for: Panoramic visual statistics shape retina-wide organization
of receptive fields'
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '12226'
abstract:
- lang: eng
text: "Background: Biases of DNA repair can shape the nucleotide landscape of genomes
at evolutionary timescales. The molecular mechanisms of those biases are still
poorly understood because it is difficult to isolate the contributions of DNA
repair from those of DNA damage.\r\n\r\nResults: Here, we develop a genome-wide
assay whereby the same DNA lesion is repaired in different genomic contexts. We
insert thousands of barcoded transposons carrying a reporter of DNA mismatch repair
in the genome of mouse embryonic stem cells. Upon inducing a double-strand break
between tandem repeats, a mismatch is generated if the break is repaired through
single-strand annealing. The resolution of the mismatch showed a 60–80% bias in
favor of the strand with the longest 3′ flap. The location of the lesion in the
genome and the type of mismatch had little influence on the bias. Instead, we
observe a complete reversal of the bias when the longest 3′ flap is moved to the
opposite strand by changing the position of the double-strand break in the reporter.\r\n\r\nConclusions:
These results suggest that the processing of the double-strand break has a major
influence on the repair of mismatches during single-strand annealing."
acknowledgement: We acknowledge the financial support of the Natural Sciences and
Engineering Research Council of Canada (NSERC RGPIN-2020-06377), the Spanish Ministry
of Economy, Industry and Competitiveness (“Centro de Excelencia Severo Ochoa 2013-2017”,
Plan Estatal PGC2018-099807-B-I00), of the CERCA Programme/Generalitat de Catalunya,
and of the European Research Council (Synergy Grant 609989). VOP was supported by
the European Union’s Horizon 2020 research and innovation program under the Marie
Skłodowska-Curie programme (665385). We also acknowledge the support of the Spanish
Ministry of Economy and Competitiveness (MEIC) to the EMBL partnership.
article_number: '93'
article_processing_charge: No
article_type: original
author:
- first_name: Victoria
full_name: Pokusaeva, Victoria
id: 3184041C-F248-11E8-B48F-1D18A9856A87
last_name: Pokusaeva
orcid: 0000-0001-7660-444X
- first_name: Aránzazu Rosado
full_name: Diez, Aránzazu Rosado
last_name: Diez
- first_name: Lorena
full_name: Espinar, Lorena
last_name: Espinar
- first_name: Albert Torelló
full_name: Pérez, Albert Torelló
last_name: Pérez
- first_name: Guillaume J.
full_name: Filion, Guillaume J.
last_name: Filion
citation:
ama: Pokusaeva V, Diez AR, Espinar L, Pérez AT, Filion GJ. Strand asymmetry influences
mismatch resolution during single-strand annealing. Genome Biology. 2022;23.
doi:10.1186/s13059-022-02665-3
apa: Pokusaeva, V., Diez, A. R., Espinar, L., Pérez, A. T., & Filion, G. J.
(2022). Strand asymmetry influences mismatch resolution during single-strand annealing.
Genome Biology. Springer Nature. https://doi.org/10.1186/s13059-022-02665-3
chicago: Pokusaeva, Victoria, Aránzazu Rosado Diez, Lorena Espinar, Albert Torelló
Pérez, and Guillaume J. Filion. “Strand Asymmetry Influences Mismatch Resolution
during Single-Strand Annealing.” Genome Biology. Springer Nature, 2022.
https://doi.org/10.1186/s13059-022-02665-3.
ieee: V. Pokusaeva, A. R. Diez, L. Espinar, A. T. Pérez, and G. J. Filion, “Strand
asymmetry influences mismatch resolution during single-strand annealing,” Genome
Biology, vol. 23. Springer Nature, 2022.
ista: Pokusaeva V, Diez AR, Espinar L, Pérez AT, Filion GJ. 2022. Strand asymmetry
influences mismatch resolution during single-strand annealing. Genome Biology.
23, 93.
mla: Pokusaeva, Victoria, et al. “Strand Asymmetry Influences Mismatch Resolution
during Single-Strand Annealing.” Genome Biology, vol. 23, 93, Springer
Nature, 2022, doi:10.1186/s13059-022-02665-3.
short: V. Pokusaeva, A.R. Diez, L. Espinar, A.T. Pérez, G.J. Filion, Genome Biology
23 (2022).
date_created: 2023-01-16T09:48:44Z
date_published: 2022-04-12T00:00:00Z
date_updated: 2025-03-31T16:01:11Z
day: '12'
ddc:
- '570'
department:
- _id: MaJö
doi: 10.1186/s13059-022-02665-3
ec_funded: 1
external_id:
isi:
- '000781953800001'
pmid:
- '35414014'
file:
- access_level: open_access
checksum: 17bb091fec04d82ba20a3458c4cfd2bd
content_type: application/pdf
creator: dernst
date_created: 2023-01-27T09:01:40Z
date_updated: 2023-01-27T09:01:40Z
file_id: '12419'
file_name: 2022_GenomeBiology_Pokusaeva.pdf
file_size: 4939342
relation: main_file
success: 1
file_date_updated: 2023-01-27T09:01:40Z
has_accepted_license: '1'
intvolume: ' 23'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Genome Biology
publication_identifier:
issn:
- 1474-760X
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: software
url: 'https://github.com/cellcomplexitylab/strand_asymmetry '
- relation: software
url: https://hub.docker.com/r/gui11aume/strand_asymmetry
scopus_import: '1'
status: public
title: Strand asymmetry influences mismatch resolution during single-strand annealing
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 23
year: '2022'
...
---
_id: '12288'
abstract:
- lang: eng
text: To understand the function of neuronal circuits, it is crucial to disentangle
the connectivity patterns within the network. However, most tools currently used
to explore connectivity have low throughput, low selectivity, or limited accessibility.
Here, we report the development of an improved packaging system for the production
of the highly neurotropic RVdGenvA-CVS-N2c rabies viral vectors, yielding titers
orders of magnitude higher with no background contamination, at a fraction of
the production time, while preserving the efficiency of transsynaptic labeling.
Along with the production pipeline, we developed suites of ‘starter’ AAV and bicistronic
RVdG-CVS-N2c vectors, enabling retrograde labeling from a wide range of neuronal
populations, tailored for diverse experimental requirements. We demonstrate the
power and flexibility of the new system by uncovering hidden local and distal
inhibitory connections in the mouse hippocampal formation and by imaging the functional
properties of a cortical microcircuit across weeks. Our novel production pipeline
provides a convenient approach to generate new rabies vectors, while our toolkit
flexibly and efficiently expands the current capacity to label, manipulate and
image the neuronal activity of interconnected neuronal circuits in vitro and in
vivo.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank F Marr for technical assistance, A Murray for RVdG-CVS-N2c
viruses and Neuro2A packaging cell-lines and J Watson for reading the manuscript.
This research was supported by the Scientific Service Units (SSU) of IST-Austria
through resources provided by the Imaging and Optics Facility (IOF) and the Preclinical
Facility (PCF). This project was funded by the European Research Council (ERC) under
the European Union’s Horizon 2020 research and innovation programme (ERC advanced
grant No 692692, PJ, ERC starting grant No 756502, MJ), the Fond zur Förderung der
Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award, PJ), the Human Frontier
Science Program (LT000256/2018-L, AS) and EMBO (ALTF 1098-2017, AS).
article_number: '79848'
article_processing_charge: No
article_type: original
author:
- first_name: Anton L
full_name: Sumser, Anton L
id: 3320A096-F248-11E8-B48F-1D18A9856A87
last_name: Sumser
orcid: 0000-0002-4792-1881
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Yoav
full_name: Ben Simon, Yoav
id: 43DF3136-F248-11E8-B48F-1D18A9856A87
last_name: Ben Simon
citation:
ama: Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. Fast, high-throughput production
of improved rabies viral vectors for specific, efficient and versatile transsynaptic
retrograde labeling. eLife. 2022;11. doi:10.7554/elife.79848
apa: Sumser, A. L., Jösch, M. A., Jonas, P. M., & Ben Simon, Y. (2022). Fast,
high-throughput production of improved rabies viral vectors for specific, efficient
and versatile transsynaptic retrograde labeling. ELife. eLife Sciences
Publications. https://doi.org/10.7554/elife.79848
chicago: Sumser, Anton L, Maximilian A Jösch, Peter M Jonas, and Yoav Ben Simon.
“Fast, High-Throughput Production of Improved Rabies Viral Vectors for Specific,
Efficient and Versatile Transsynaptic Retrograde Labeling.” ELife. eLife
Sciences Publications, 2022. https://doi.org/10.7554/elife.79848.
ieee: A. L. Sumser, M. A. Jösch, P. M. Jonas, and Y. Ben Simon, “Fast, high-throughput
production of improved rabies viral vectors for specific, efficient and versatile
transsynaptic retrograde labeling,” eLife, vol. 11. eLife Sciences Publications,
2022.
ista: Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. 2022. Fast, high-throughput production
of improved rabies viral vectors for specific, efficient and versatile transsynaptic
retrograde labeling. eLife. 11, 79848.
mla: Sumser, Anton L., et al. “Fast, High-Throughput Production of Improved Rabies
Viral Vectors for Specific, Efficient and Versatile Transsynaptic Retrograde Labeling.”
ELife, vol. 11, 79848, eLife Sciences Publications, 2022, doi:10.7554/elife.79848.
short: A.L. Sumser, M.A. Jösch, P.M. Jonas, Y. Ben Simon, ELife 11 (2022).
corr_author: '1'
date_created: 2023-01-16T10:04:15Z
date_published: 2022-09-15T00:00:00Z
date_updated: 2025-04-15T08:29:05Z
day: '15'
ddc:
- '570'
department:
- _id: MaJö
- _id: PeJo
doi: 10.7554/elife.79848
ec_funded: 1
external_id:
isi:
- '000892204300001'
pmid:
- '36040301'
file:
- access_level: open_access
checksum: 5a2a65e3e7225090c3d8199f3bbd7b7b
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T11:50:53Z
date_updated: 2023-01-30T11:50:53Z
file_id: '12463'
file_name: 2022_eLife_Sumser.pdf
file_size: 8506811
relation: main_file
success: 1
file_date_updated: 2023-01-30T11:50:53Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: Synaptic communication in neuronal microcircuits
- _id: 266D407A-B435-11E9-9278-68D0E5697425
grant_number: LT000256
name: Neuronal networks of salience and spatial detection in the murine superior
colliculus
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1098-2017
name: Connecting sensory with motor processing in the superior colliculus
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fast, high-throughput production of improved rabies viral vectors for specific,
efficient and versatile transsynaptic retrograde labeling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2022'
...
---
_id: '15268'
abstract:
- lang: eng
text: Apolipoprotein A‐I (apoA‐I) has a key function in the reverse cholesterol
transport. However, aggregation of apoA‐I single point mutants can lead to hereditary
amyloid pathology. Although several studies have tackled the biophysical and structural
consequences introduced by these mutations, there is little information addressing
the relationship between the evolutionary and structural features that contribute
to the amyloid behavior of apoA‐I. We combined evolutionary studies, in silico
mutagenesis and molecular dynamics (MD) simulations to provide a comprehensive
analysis of the conservation and pathogenic role of the aggregation‐prone regions
(APRs) present in apoA‐I. Sequence analysis demonstrated that among the four amyloidogenic
regions described for human apoA‐I, only two (APR1 and APR4) are evolutionary
conserved across different species of Sarcopterygii. Moreover, stability analysis
carried out with the FoldX engine showed that APR1 contributes to the marginal
stability of apoA‐I. Structural properties of full‐length apoA‐I models suggest
that aggregation is avoided by placing APRs into highly packed and rigid portions
of its native fold. Compared to silent variants extracted from the gnomAD database,
the thermodynamic and pathogenic impact of amyloid mutations showed evidence of
a higher destabilizing effect. MD simulations of the amyloid variant G26R evidenced
the partial unfolding of the alpha‐helix bundle with the concomitant exposure
of APR1 to the solvent, suggesting an insight into the early steps involved in
its aggregation. Our findings highlight APR1 as a relevant component for apoA‐I
structural integrity and emphasize a destabilizing effect of amyloid variants
that leads to the exposure of this region.
article_processing_charge: No
article_type: original
author:
- first_name: Romina A.
full_name: Gisonno, Romina A.
last_name: Gisonno
- first_name: Tomas
full_name: Masson, Tomas
id: 93ac43e8-8599-11eb-9b86-f6efb0a4c207
last_name: Masson
orcid: 0000-0002-2634-6283
- first_name: Nahuel A.
full_name: Ramella, Nahuel A.
last_name: Ramella
- first_name: Exequiel E.
full_name: Barrera, Exequiel E.
last_name: Barrera
- first_name: Víctor
full_name: Romanowski, Víctor
last_name: Romanowski
- first_name: M. Alejandra
full_name: Tricerri, M. Alejandra
last_name: Tricerri
citation:
ama: 'Gisonno RA, Masson T, Ramella NA, Barrera EE, Romanowski V, Tricerri MA. Evolutionary
and structural constraints influencing apolipoprotein A‐I amyloid behavior. Proteins:
Structure, Function, and Bioinformatics. 2022;90(1):258-269. doi:10.1002/prot.26217'
apa: 'Gisonno, R. A., Masson, T., Ramella, N. A., Barrera, E. E., Romanowski, V.,
& Tricerri, M. A. (2022). Evolutionary and structural constraints influencing
apolipoprotein A‐I amyloid behavior. Proteins: Structure, Function, and Bioinformatics.
Wiley. https://doi.org/10.1002/prot.26217'
chicago: 'Gisonno, Romina A., Tomas Masson, Nahuel A. Ramella, Exequiel E. Barrera,
Víctor Romanowski, and M. Alejandra Tricerri. “Evolutionary and Structural Constraints
Influencing Apolipoprotein A‐I Amyloid Behavior.” Proteins: Structure, Function,
and Bioinformatics. Wiley, 2022. https://doi.org/10.1002/prot.26217.'
ieee: 'R. A. Gisonno, T. Masson, N. A. Ramella, E. E. Barrera, V. Romanowski, and
M. A. Tricerri, “Evolutionary and structural constraints influencing apolipoprotein
A‐I amyloid behavior,” Proteins: Structure, Function, and Bioinformatics,
vol. 90, no. 1. Wiley, pp. 258–269, 2022.'
ista: 'Gisonno RA, Masson T, Ramella NA, Barrera EE, Romanowski V, Tricerri MA.
2022. Evolutionary and structural constraints influencing apolipoprotein A‐I amyloid
behavior. Proteins: Structure, Function, and Bioinformatics. 90(1), 258–269.'
mla: 'Gisonno, Romina A., et al. “Evolutionary and Structural Constraints Influencing
Apolipoprotein A‐I Amyloid Behavior.” Proteins: Structure, Function, and Bioinformatics,
vol. 90, no. 1, Wiley, 2022, pp. 258–69, doi:10.1002/prot.26217.'
short: 'R.A. Gisonno, T. Masson, N.A. Ramella, E.E. Barrera, V. Romanowski, M.A.
Tricerri, Proteins: Structure, Function, and Bioinformatics 90 (2022) 258–269.'
corr_author: '1'
date_created: 2024-04-03T07:49:53Z
date_published: 2022-01-01T00:00:00Z
date_updated: 2024-10-09T21:08:44Z
day: '01'
department:
- _id: MaJö
doi: 10.1002/prot.26217
external_id:
pmid:
- '34414600'
intvolume: ' 90'
issue: '1'
keyword:
- Molecular Biology
- Biochemistry
- Structural Biology
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2020.09.18.304337
month: '01'
oa: 1
oa_version: Preprint
page: 258-269
pmid: 1
publication: 'Proteins: Structure, Function, and Bioinformatics'
publication_identifier:
eissn:
- 1097-0134
issn:
- 0887-3585
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: Evolutionary and structural constraints influencing apolipoprotein A‐I amyloid
behavior
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 90
year: '2022'
...
---
_id: '17071'
abstract:
- lang: eng
text: "The eukaryotic nucleus protects the genome and is enclosed by the two membranes
of the nuclear envelope. Nuclear pore complexes (NPCs) perforate the nuclear envelope
to facilitate nucleocytoplasmic transport. With a molecular weight of ∼120 MDa,
the human NPC is one of the largest protein complexes. Its ~1000 proteins are
taken in multiple copies from a set of about 30 distinct nucleoporins (NUPs).
They can be roughly categorized into two classes. Scaffold NUPs contain folded
domains and form a cylindrical scaffold architecture around a central channel.
Intrinsically disordered NUPs line the scaffold and extend into the central channel,
where they interact with cargo complexes. The NPC architecture is highly dynamic.
It responds to changes in nuclear envelope tension with conformational breathing
that manifests in dilation and constriction movements. Elucidating the scaffold
architecture, ultimately at atomic resolution, will be important for gaining a
more precise understanding of NPC function and dynamics but imposes a substantial
challenge for structural biologists.\r\nConsiderable progress has been made toward
this goal by a joint effort in the field. A synergistic combination of complementary
approaches has turned out to be critical. In situ structural biology techniques
were used to reveal the overall layout of the NPC scaffold that defines the spatial
reference for molecular modeling. High-resolution structures of many NUPs were
determined in vitro. Proteomic analysis and extensive biochemical work unraveled
the interaction network of NUPs. Integrative modeling has been used to combine
the different types of data, resulting in a rough outline of the NPC scaffold.
Previous structural models of the human NPC, however, were patchy and limited
in accuracy owing to several challenges: (i) Many of the high-resolution structures
of individual NUPs have been solved from distantly related species and, consequently,
do not comprehensively cover their human counterparts. (ii) The scaffold is interconnected
by a set of intrinsically disordered linker NUPs that are not straightforwardly
accessible to common structural biology techniques. (iii) The NPC scaffold intimately
embraces the fused inner and outer nuclear membranes in a distinctive topology
and cannot be studied in isolation. (iv) The conformational dynamics of scaffold
NUPs limits the resolution achievable in structure determination.\r\nIn this study,
we used artificial intelligence (AI)–based prediction to generate an extensive
repertoire of structural models of human NUPs and their subcomplexes. The resulting
models cover various domains and interfaces that so far remained structurally
uncharacterized. Benchmarking against previous and unpublished x-ray and cryo–electron
microscopy structures revealed unprecedented accuracy. We obtained well-resolved
cryo–electron tomographic maps of both the constricted and dilated conformational
states of the human NPC. Using integrative modeling, we fitted the structural
models of individual NUPs into the cryo–electron microscopy maps. We explicitly
included several linker NUPs and traced their trajectory through the NPC scaffold.
We elucidated in great detail how membrane-associated and transmembrane NUPs
are distributed across the fusion topology of both nuclear membranes. The resulting
architectural model increases the structural coverage of the human NPC scaffold
by about twofold. We extensively validated our model against both earlier and
new experimental data. The completeness of our model has enabled microsecond-long
coarse-grained molecular dynamics simulations of the NPC scaffold within an explicit
membrane environment and solvent. These simulations reveal that the NPC scaffold
prevents the constriction of the otherwise stable double-membrane fusion pore
to small diameters in the absence of membrane tension\r\nOur 70-MDa atomically
resolved model covers >90% of the human NPC scaffold. It captures conformational
changes that occur during dilation and constriction. It also reveals the precise
anchoring sites for intrinsically disordered NUPs, the identification of which
is a prerequisite for a complete and dynamic model of the NPC. Our study exemplifies
how AI-based structure prediction may accelerate the elucidation of subcellular
architecture at atomic resolution."
acknowledgement: "We acknowledge support from the Electron Microscopy Core Facility
(EMCF) and IT services of European Molecular Biology Laboratory (EMBL) Heidelberg.
We thank S. Welsch at the Central Electron Microscopy Facility of the Max Planck
Institute of Biophysics for technical expertise. We thank T. Hoffman and R. Alves
for help with the AlphaFold installation.\r\nFunding: M.B. acknowledges funding
by EMBL, the Max Planck Society, and the European Research Council (ComplexAssembly
724349). J.K. acknowledges funding from the Federal Ministry of Education and Research
of Germany (FKZ 031L0100). The work by M.S. and G.H. on computer simulations was
supported by the Max Planck Society. M.S. was supported by the EMBL Interdisciplinary
Postdoc Programme under Marie Curie COFUND actions. M.S. and G.H. were supported
by the Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz
(LOEWE) DynaMem program of the State of Hessen."
article_number: abm9506
article_processing_charge: No
article_type: original
author:
- first_name: Shyamal
full_name: Mosalaganti, Shyamal
last_name: Mosalaganti
- first_name: Agnieszka
full_name: Obarska-Kosinska, Agnieszka
last_name: Obarska-Kosinska
- first_name: Marc
full_name: Siggel, Marc
last_name: Siggel
- first_name: Reiya
full_name: Taniguchi, Reiya
last_name: Taniguchi
- first_name: Beata
full_name: Turoňová, Beata
last_name: Turoňová
- first_name: Christian E.
full_name: Zimmerli, Christian E.
last_name: Zimmerli
- first_name: Katarzyna
full_name: Buczak, Katarzyna
last_name: Buczak
- first_name: Florian
full_name: Schmidt, Florian
id: A2EF226A-AF19-11E9-924C-0525E6697425
last_name: Schmidt
- first_name: Erica
full_name: Margiotta, Erica
last_name: Margiotta
- first_name: Marie-Therese
full_name: Mackmull, Marie-Therese
last_name: Mackmull
- first_name: Wim J. H.
full_name: Hagen, Wim J. H.
last_name: Hagen
- first_name: Gerhard
full_name: Hummer, Gerhard
last_name: Hummer
- first_name: Jan
full_name: Kosinski, Jan
last_name: Kosinski
- first_name: Martin
full_name: Beck, Martin
last_name: Beck
citation:
ama: Mosalaganti S, Obarska-Kosinska A, Siggel M, et al. AI-based structure prediction
empowers integrative structural analysis of human nuclear pores. Science.
2022;376(6598). doi:10.1126/science.abm9506
apa: Mosalaganti, S., Obarska-Kosinska, A., Siggel, M., Taniguchi, R., Turoňová,
B., Zimmerli, C. E., … Beck, M. (2022). AI-based structure prediction empowers
integrative structural analysis of human nuclear pores. Science. American
Association for the Advancement of Science. https://doi.org/10.1126/science.abm9506
chicago: Mosalaganti, Shyamal, Agnieszka Obarska-Kosinska, Marc Siggel, Reiya Taniguchi,
Beata Turoňová, Christian E. Zimmerli, Katarzyna Buczak, et al. “AI-Based Structure
Prediction Empowers Integrative Structural Analysis of Human Nuclear Pores.” Science.
American Association for the Advancement of Science, 2022. https://doi.org/10.1126/science.abm9506.
ieee: S. Mosalaganti et al., “AI-based structure prediction empowers integrative
structural analysis of human nuclear pores,” Science, vol. 376, no. 6598.
American Association for the Advancement of Science, 2022.
ista: Mosalaganti S, Obarska-Kosinska A, Siggel M, Taniguchi R, Turoňová B, Zimmerli
CE, Buczak K, Schmidt F, Margiotta E, Mackmull M-T, Hagen WJH, Hummer G, Kosinski
J, Beck M. 2022. AI-based structure prediction empowers integrative structural
analysis of human nuclear pores. Science. 376(6598), abm9506.
mla: Mosalaganti, Shyamal, et al. “AI-Based Structure Prediction Empowers Integrative
Structural Analysis of Human Nuclear Pores.” Science, vol. 376, no. 6598,
abm9506, American Association for the Advancement of Science, 2022, doi:10.1126/science.abm9506.
short: S. Mosalaganti, A. Obarska-Kosinska, M. Siggel, R. Taniguchi, B. Turoňová,
C.E. Zimmerli, K. Buczak, F. Schmidt, E. Margiotta, M.-T. Mackmull, W.J.H. Hagen,
G. Hummer, J. Kosinski, M. Beck, Science 376 (2022).
date_created: 2024-05-29T06:12:02Z
date_published: 2022-06-10T00:00:00Z
date_updated: 2024-07-31T12:10:32Z
day: '10'
department:
- _id: MaJö
doi: 10.1126/science.abm9506
external_id:
pmid:
- '35679397'
intvolume: ' 376'
issue: '6598'
language:
- iso: eng
month: '06'
oa_version: None
pmid: 1
publication: Science
publication_identifier:
eissn:
- 1095-9203
issn:
- 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: AI-based structure prediction empowers integrative structural analysis of human
nuclear pores
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 376
year: '2022'
...