[{"publication_status":"published","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"pmid":1,"external_id":{"pmid":["41831441"]},"corr_author":"1","author":[{"last_name":"Li","first_name":"Mingyue","id":"01f96916-0235-11eb-9379-a323192643b7","full_name":"Li, Mingyue"},{"full_name":"Rydza, Nikola","last_name":"Rydza","first_name":"Nikola"},{"full_name":"Mazur, Ewa","last_name":"Mazur","first_name":"Ewa"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","full_name":"Molnar, Gergely","last_name":"Molnar","first_name":"Gergely"},{"first_name":"Tomasz","last_name":"Nodzyński","full_name":"Nodzyński, Tomasz"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"project":[{"name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"grant_number":"E271","name":"Identification of a novel regulator in auxin canalization","_id":"bd906599-d553-11ed-ba76-abf8547645d7"}],"PlanS_conform":"1","month":"03","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"Bio"},{"_id":"LifeSc"}],"abstract":[{"text":"Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development.","lang":"eng"}],"volume":36,"date_created":"2026-03-23T15:11:16Z","date_published":"2026-03-23T00:00:00Z","file_date_updated":"2026-03-24T08:34:37Z","article_type":"original","year":"2026","intvolume":"        36","acknowledgement":"We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.","language":[{"iso":"eng"}],"issue":"6","oa":1,"article_processing_charge":"Yes (via OA deal)","type":"journal_article","ddc":["580"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Published Version","page":"1468-1480.e6","status":"public","publication_identifier":{"issn":["0960-9822"]},"doi":"10.1016/j.cub.2026.02.023","quality_controlled":"1","day":"23","OA_type":"hybrid","date_updated":"2026-03-24T08:36:40Z","_id":"21490","title":"Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization","OA_place":"publisher","file":[{"success":1,"checksum":"fe6c41fdab58a55df5f2a5860c02acdc","file_id":"21496","file_name":"2026_CurrentBiology_Li.pdf","date_updated":"2026-03-24T08:34:37Z","date_created":"2026-03-24T08:34:37Z","relation":"main_file","access_level":"open_access","creator":"dernst","file_size":12986894,"content_type":"application/pdf"}],"publisher":"Elsevier","department":[{"_id":"JiFr"}],"publication":"Current Biology","has_accepted_license":"1","citation":{"ista":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6.","chicago":"Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>.","short":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6.","apa":"Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., &#38; Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>","mla":"Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>, vol. 36, no. 6, Elsevier, 2026, p. 1468–1480.e6, doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>.","ieee":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” <i>Current Biology</i>, vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026.","ama":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. 2026;36(6):1468-1480.e6. doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>"}},{"intvolume":"       368","acknowledgement":"We gratefully acknowledge ISTA for generous financial support. B.P. acknowledges the Austrian Science Fund (PAT 1250924) and the ACS GCI Pharmaceutical Roundtable for funding; T.P.Y acknowledges the NSF(CHE-2349003) for financial support. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Lab Support Facility, Mass Spec Facility, NMR facility, and the Miba Machine Shop. We specifically thank Aikaterina Paraskevopoulou for HRMS measurements and Jan Pecak for support with ICP-OES experi-ments. NMR facilities at UW−Madison were supported by the NSF(CHE-1048642) and a generous gift from Paul J. and Margaret M. Bender. Open Access funding provided by Institute of Science and Technology Austria/KEMÖ. This study was supported by Austrian Science Fund (PAT 1250924), ACSGCI Pharmaceutical Roundtable, and National Science Foundation(CHE-2349003) and (CHE-1048642).","oa":1,"issue":"9","language":[{"iso":"eng"}],"date_created":"2026-05-03T22:01:36Z","file_date_updated":"2026-05-07T07:29:24Z","date_published":"2026-05-05T00:00:00Z","year":"2026","article_type":"original","volume":368,"month":"05","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"MassSpec"},{"_id":"NMR"},{"_id":"M-Shop"}],"abstract":[{"text":"Pyridyl motifs equipped with N-substituents can be powerful ligands for catalysis, yet their broader adoption is limited by the lack of a practical method to prepare these scaffolds. We report a modular, robust, and versatile Buchwald–Hartwig amination protocol that enables the rapid synthesis of bipyridine, phenanthroline, terpyridine, and pybox ligands bearing dialkylamine, diarylamine, and heteroaromatic N-substituents. These conditions streamline ligand library synthesis and will facilitate systematic studies in catalysis and related applications.","lang":"eng"}],"project":[{"_id":"8f1d607d-16d5-11f0-9cad-ab453295ba5e","grant_number":"PAT 1250924","name":"Photoactive ligands for transformative nickel catalysis"}],"PlanS_conform":"1","author":[{"full_name":"Petrik, Adam","id":"e273d403-329f-11ee-a353-8c34c056f8ed","first_name":"Adam","last_name":"Petrik"},{"first_name":"Aleksander","last_name":"Bena","full_name":"Bena, Aleksander","id":"4197c39e-e8ec-11ed-86cb-afed934cd664"},{"first_name":"Haralds","last_name":"Baunis","full_name":"Baunis, Haralds","id":"2eea55ec-e8ec-11ed-86cb-d9c76787acfe"},{"full_name":"Kelch, Riley M.","first_name":"Riley M.","last_name":"Kelch"},{"full_name":"Yoon, Tehshik P.","last_name":"Yoon","first_name":"Tehshik P."},{"last_name":"Pieber","first_name":"Bartholomäus","id":"93e5e5b2-0da6-11ed-8a41-af589a024726","orcid":"0000-0001-8689-388X","full_name":"Pieber, Bartholomäus"}],"publication_status":"published","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_number":"e70417","corr_author":"1","file":[{"relation":"main_file","date_created":"2026-05-07T07:29:24Z","access_level":"open_access","creator":"dernst","file_size":437184,"content_type":"application/pdf","success":1,"file_id":"21833","checksum":"afe9752977898642c903abdc70b4a283","file_name":"2026_AdvSynthCatal_Petrik.pdf","date_updated":"2026-05-07T07:29:24Z"}],"publisher":"Wiley","department":[{"_id":"BaPi"},{"_id":"GradSch"}],"has_accepted_license":"1","publication":"Advanced Synthesis & Catalysis","citation":{"ieee":"A. Petrik, A. Bena, H. Baunis, R. M. Kelch, T. P. Yoon, and B. Pieber, “Facile access to N-substituted pyridyl ligands,” <i>Advanced Synthesis &#38; Catalysis</i>, vol. 368, no. 9. Wiley, 2026.","apa":"Petrik, A., Bena, A., Baunis, H., Kelch, R. M., Yoon, T. P., &#38; Pieber, B. (2026). Facile access to N-substituted pyridyl ligands. <i>Advanced Synthesis &#38; Catalysis</i>. Wiley. <a href=\"https://doi.org/10.1002/adsc.70417\">https://doi.org/10.1002/adsc.70417</a>","mla":"Petrik, Adam, et al. “Facile Access to N-Substituted Pyridyl Ligands.” <i>Advanced Synthesis &#38; Catalysis</i>, vol. 368, no. 9, e70417, Wiley, 2026, doi:<a href=\"https://doi.org/10.1002/adsc.70417\">10.1002/adsc.70417</a>.","ama":"Petrik A, Bena A, Baunis H, Kelch RM, Yoon TP, Pieber B. Facile access to N-substituted pyridyl ligands. <i>Advanced Synthesis &#38; Catalysis</i>. 2026;368(9). doi:<a href=\"https://doi.org/10.1002/adsc.70417\">10.1002/adsc.70417</a>","chicago":"Petrik, Adam, Aleksander Bena, Haralds Baunis, Riley M. Kelch, Tehshik P. Yoon, and Bartholomäus Pieber. “Facile Access to N-Substituted Pyridyl Ligands.” <i>Advanced Synthesis &#38; Catalysis</i>. Wiley, 2026. <a href=\"https://doi.org/10.1002/adsc.70417\">https://doi.org/10.1002/adsc.70417</a>.","ista":"Petrik A, Bena A, Baunis H, Kelch RM, Yoon TP, Pieber B. 2026. Facile access to N-substituted pyridyl ligands. Advanced Synthesis &#38; Catalysis. 368(9), e70417.","short":"A. Petrik, A. Bena, H. Baunis, R.M. Kelch, T.P. Yoon, B. Pieber, Advanced Synthesis &#38; Catalysis 368 (2026)."},"title":"Facile access to N-substituted pyridyl ligands","_id":"21776","OA_place":"publisher","OA_type":"hybrid","date_updated":"2026-05-07T07:33:33Z","day":"05","status":"public","doi":"10.1002/adsc.70417","publication_identifier":{"issn":["1615-4150"],"eissn":["1615-4169"]},"quality_controlled":"1","oa_version":"Published Version","scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["540"],"article_processing_charge":"Yes (via OA deal)","type":"journal_article"},{"citation":{"ama":"Li M, Chodasiewicz M, Muraleedharan M, et al. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. <i>Science Advances</i>. 2026;12(19). doi:<a href=\"https://doi.org/10.1126/sciadv.aea7828\">10.1126/sciadv.aea7828</a>","mla":"Li, Mingyue, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” <i>Science Advances</i>, vol. 12, no. 19, aea7828, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aea7828\">10.1126/sciadv.aea7828</a>.","apa":"Li, M., Chodasiewicz, M., Muraleedharan, M., Lopez, I. M., Gorka, M., Kerber, O., … Friml, J. (2026). Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.aea7828\">https://doi.org/10.1126/sciadv.aea7828</a>","ieee":"M. Li <i>et al.</i>, “Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants,” <i>Science Advances</i>, vol. 12, no. 19. AAAS, 2026.","short":"M. Li, M. Chodasiewicz, M. Muraleedharan, I.M. Lopez, M. Gorka, O. Kerber, S.S. Alotaibi, A.D.L. Nelson, R. Lenobel, J. Friedecká, A. Skirycz, J. Friml, Science Advances 12 (2026).","ista":"Li M, Chodasiewicz M, Muraleedharan M, Lopez IM, Gorka M, Kerber O, Alotaibi SS, Nelson ADL, Lenobel R, Friedecká J, Skirycz A, Friml J. 2026. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. 12(19), aea7828.","chicago":"Li, Mingyue, Monika Chodasiewicz, Malavika Muraleedharan, Israel M. Lopez, Michal Gorka, Olga Kerber, Saqer S. Alotaibi, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” <i>Science Advances</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/sciadv.aea7828\">https://doi.org/10.1126/sciadv.aea7828</a>."},"has_accepted_license":"1","publication":"Science Advances","department":[{"_id":"JiFr"}],"publisher":"AAAS","file":[{"success":1,"file_id":"21941","checksum":"75b8ef2db078652c750e34e9cd98a808","file_name":"2026_ScienceAdv_Li2.pdf","date_updated":"2026-06-02T14:33:55Z","date_created":"2026-06-02T14:33:55Z","relation":"main_file","access_level":"open_access","creator":"dernst","file_size":2014452,"content_type":"application/pdf"}],"OA_place":"publisher","_id":"21914","title":"Biogenesis and downstream effects of 3',5' and 2',3' cAMP isomers in plants","date_updated":"2026-06-02T14:36:41Z","OA_type":"gold","day":"08","quality_controlled":"1","doi":"10.1126/sciadv.aea7828","publication_identifier":{"eissn":["2375-2548"]},"status":"public","scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Published Version","ddc":["580"],"type":"journal_article","article_processing_charge":"Yes","issue":"19","oa":1,"acknowledgement":" We thank J. Chai and D. Yu for providing the MBP-fused L7TIR plasmid and K. Jaworski (Nicolaus Copernicus University) for the GST-­HpAC1 plasmid. We also thank M. Randuch and L. Fiedler for providing vectors for recombinant AFB5 and ADCY. We are also grateful to E. Dutkiewicz, L. Trübestein, N. Krasnici and A. Michaelis for excellent technical\r\nassistance. We acknowledge the support of the LSF Mass Spectrometry Service and the Lab\r\nSupport Facility at the Institute of Science and Technology Austria for their contributions,\r\nincluding consultation on size exclusion chromatography, LC/MS experimental design,\r\nmetabolomics sample preparation, LC/MS method optimization, data acquisition, raw data\r\nanalysis, and absolute quantification. This project is supported by the European\r\nResearch Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogram (101142681 CYNIPS) and Austrian Science Fund (FWF; P 37051-B), both to J.Friml.\r\nWe acknowledge the generous support of the Taif University Researchers Supporting\r\nProject: TURSP-­HC2022/02 and Max-Planck-Society to A.S. ","language":[{"iso":"eng"}],"intvolume":"        12","article_type":"original","year":"2026","date_published":"2026-05-08T00:00:00Z","file_date_updated":"2026-06-02T14:33:55Z","date_created":"2026-05-24T22:01:31Z","volume":12,"abstract":[{"text":"Cyclic adenosine monophosphate (cAMP) is a fundamental second messenger involved in diverse signaling pathways across both animals and plants. While the role of 3′,5′-cAMP has been extensively characterized, the biological significance of its structural isomer, 2′,3′-cAMP, remains largely unexplored, particularly in plants. Here, we show that 2′,3′-cAMP and 3′,5′-cAMP represent parallel signaling systems in Arabidopsis thaliana, with different enzymatic origins and largely distinct downstream effects. In vitro enzymatic assays show that plant adenylate cyclases (ACs), including AFB5 and HpAC1, produce specifically 3′,5′-cAMP from ATP, whereas the TIR domain of protein L7 also catalyzes the formation of 2′,3′-cAMP from RNA. Comprehensive multiomics analyses reveal that two isomers elicit distinct yet partially overlapping metabolic, proteomic, and transcriptional response: 2′,3′-cAMP activates broad, stress-adaptive gene expression reprogramming, while 3′,5′-cAMP fine-tunes responses related to nutrient status and cellular homeostasis. Our findings establish the existence of dual cAMP signaling systems in plants, each with specialized functions and provide insights into the complex regulatory networks governing plant physiology.","lang":"eng"}],"acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"LifeSc"}],"month":"05","PlanS_conform":"1","DOAJ_listed":"1","project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"author":[{"full_name":"Li, Mingyue","id":"01f96916-0235-11eb-9379-a323192643b7","first_name":"Mingyue","last_name":"Li"},{"full_name":"Chodasiewicz, Monika","first_name":"Monika","last_name":"Chodasiewicz"},{"first_name":"Malavika","last_name":"Muraleedharan","full_name":"Muraleedharan, Malavika"},{"full_name":"Lopez, Israel M.","first_name":"Israel M.","last_name":"Lopez"},{"first_name":"Michal","last_name":"Gorka","full_name":"Gorka, Michal"},{"last_name":"Kerber","first_name":"Olga","full_name":"Kerber, Olga"},{"full_name":"Alotaibi, Saqer S.","first_name":"Saqer S.","last_name":"Alotaibi"},{"full_name":"Nelson, Andrew D.L.","last_name":"Nelson","first_name":"Andrew D.L."},{"full_name":"Lenobel, Rene","last_name":"Lenobel","first_name":"Rene"},{"full_name":"Friedecká, Jaroslava","first_name":"Jaroslava","last_name":"Friedecká"},{"last_name":"Skirycz","first_name":"Aleksandra","full_name":"Skirycz, Aleksandra"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"corr_author":"1","external_id":{"pmid":["42102187"]},"article_number":"aea7828","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"pmid":1,"publication_status":"published"},{"project":[{"_id":"264E56E2-B435-11E9-9278-68D0E5697425","name":"Molecular Mechanisms Regulating Gliogenesis in the Neocortex","grant_number":"M02416","call_identifier":"FWF"},{"_id":"25D61E48-B435-11E9-9278-68D0E5697425","grant_number":"618444","call_identifier":"FP7","name":"Molecular Mechanisms of Cerebral Cortex Development"},{"_id":"260018B0-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"725780","name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development"}],"author":[{"id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","orcid":"0000-0002-5615-5277","full_name":"Villalba Requena, Ana","last_name":"Villalba Requena","first_name":"Ana"},{"id":"2E26DF60-F248-11E8-B48F-1D18A9856A87","full_name":"Beattie, Robert J","orcid":"0000-0002-8483-8753","last_name":"Beattie","first_name":"Robert J"},{"first_name":"Florian","last_name":"Pauler","orcid":"0000-0002-7462-0048","full_name":"Pauler, Florian","id":"48EA0138-F248-11E8-B48F-1D18A9856A87"},{"id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","full_name":"Streicher, Carmen","last_name":"Streicher","first_name":"Carmen"},{"first_name":"Osvaldo","last_name":"Miranda","orcid":"0000-0001-6618-6889","full_name":"Miranda, Osvaldo","id":"862A3C56-A8BF-11E9-B4FA-D9E3E5697425"},{"full_name":"Krausgruber, Thomas","first_name":"Thomas","last_name":"Krausgruber"},{"full_name":"Senekowitsch, Martin","last_name":"Senekowitsch","first_name":"Martin"},{"full_name":"Farlik, Matthias","first_name":"Matthias","last_name":"Farlik"},{"full_name":"Bock, Christoph","first_name":"Christoph","last_name":"Bock"},{"full_name":"Rülicke, Thomas","last_name":"Rülicke","first_name":"Thomas"},{"orcid":"0000-0003-2279-1061","full_name":"Hippenmeyer, Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","first_name":"Simon","last_name":"Hippenmeyer"}],"publication_status":"submitted","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)"},"acknowledgement":"We thank A. Heger (IST Austria Preclinical Facility), A. Sommer (VBCF GmbH, NGS Unit), and A.\r\nNicolas (IST Austria Lab Support Facility / Mass Spectrometry Facility) for technical support; K. Ferencak,\r\nI. Aykara, P. Hirschfeld, E. Fisher, S. Laukoter, L. Andersen for initial experiments and/or assistance; and\r\nall members of the Hippenmeyer lab for discussion. This research was supported by the Scientific Service\r\nUnits (SSU) of IST Austria through resources provided by the Imaging and Optics- (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). R.B. received support from FWF Meitner-Programm (M 2416). This\r\nwork was also supported by IST Austria institutional funds; the People Programme (Marie Curie Actions)\r\nof the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement\r\nNo 618444 to S.H., and the European Research Council (ERC) under the European Union’s Horizon 2020\r\nresearch and innovation programme (grant agreement No 725780 LinPro) to S.H.","license":"https://creativecommons.org/licenses/by-nc/4.0/","oa":1,"language":[{"iso":"eng"}],"date_published":"2026-05-05T00:00:00Z","year":"2026","date_created":"2026-06-09T08:08:18Z","abstract":[{"lang":"eng","text":"The generation of faithful cell-type diversity and correct projection neuron numbers is essential for cerebral cortex development. Corticogenesis is however susceptible to genetic interference of critical signaling pathways, including mutations in Mtor/Rptor that lead to microcephaly. How the loss of Rptor/mTORC1 function affects cortical developmental programs, at single cell level, is still unknown. Here, we utilized Mosaic Analysis with Double Markers (MADM) technology to probe Rptor gene function upon sparse single cell- or global tissue-wide ablation. We found that tissue-wide effects drive the etiology of cortical microcephaly upon loss of Rptor, rather than deficits in projection neuron genesis. Conversely, Rptor function is cell-autonomously required for postnatal projection neuron survival in a highly cell-type-specific manner. Collectively, our results suggest that the fine balance of precise cell-type-specific cell-autonomous Rptor/mTORC1 function in concert with non-cell-autonomous tissue-wide effects is essential for the development of a properly-sized cerebral cortex with accurate projection neuron diversity."}],"month":"05","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"MassSpec"},{"_id":"Bio"}],"doi":"10.64898/2026.05.01.722172","status":"public","ec_funded":1,"oa_version":"Preprint","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"type":"preprint","article_processing_charge":"No","publication":"bioRxiv","has_accepted_license":"1","citation":{"ama":"Villalba Requena A, Beattie RJ, Pauler F, et al. Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>","mla":"Villalba Requena, Ana, et al. “Mtor/Rptor Function Globally Prevents Cortical Microcephaly and Cell-Autonomously Promotes Postnatal Neuron Survival in Cell Type Specific Manner.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>.","apa":"Villalba Requena, A., Beattie, R. J., Pauler, F., Streicher, C., Miranda, O., Krausgruber, T., … Hippenmeyer, S. (n.d.). Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.05.01.722172\">https://doi.org/10.64898/2026.05.01.722172</a>","ieee":"A. Villalba Requena <i>et al.</i>, “Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner,” <i>bioRxiv</i>. .","short":"A. Villalba Requena, R.J. Beattie, F. Pauler, C. Streicher, O. Miranda, T. Krausgruber, M. Senekowitsch, M. Farlik, C. Bock, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.).","ista":"Villalba Requena A, Beattie RJ, Pauler F, Streicher C, Miranda O, Krausgruber T, Senekowitsch M, Farlik M, Bock C, Rülicke T, Hippenmeyer S. Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. bioRxiv, <a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>.","chicago":"Villalba Requena, Ana, Robert J Beattie, Florian Pauler, Carmen Streicher, Osvaldo Miranda, Thomas Krausgruber, Martin Senekowitsch, et al. “Mtor/Rptor Function Globally Prevents Cortical Microcephaly and Cell-Autonomously Promotes Postnatal Neuron Survival in Cell Type Specific Manner.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.05.01.722172\">https://doi.org/10.64898/2026.05.01.722172</a>."},"main_file_link":[{"url":"https://doi.org/10.64898/2026.05.01.722172","open_access":"1"}],"department":[{"_id":"SiHi"}],"OA_place":"repository","_id":"21962","title":"Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner","date_updated":"2026-06-16T08:45:25Z","OA_type":"green","day":"05"},{"type":"journal_article","article_processing_charge":"Yes","ddc":["570"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Published Version","scopus_import":"1","related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/ants-signal-deadly-infection/"}],"record":[{"id":"20471","relation":"research_data","status":"public"}]},"doi":"10.1038/s41467-025-66175-z","publication_identifier":{"eissn":["2041-1723"]},"quality_controlled":"1","status":"public","ec_funded":1,"day":"01","date_updated":"2026-06-10T08:50:53Z","OA_type":"gold","OA_place":"publisher","title":"Altruistic disease signalling in ant colonies","_id":"18892","has_accepted_license":"1","publication":"Nature Communications","citation":{"ama":"Dawson E, Hönigsberger M, Kampleitner N, et al. Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>","ieee":"E. Dawson <i>et al.</i>, “Altruistic disease signalling in ant colonies,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","apa":"Dawson, E., Hönigsberger, M., Kampleitner, N., Grasse, A. V., Lindorfer, L., Robb, J., … Cremer, S. (2025). Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>","mla":"Dawson, Erika, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>, vol. 16, 10511, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>.","short":"E. Dawson, M. Hönigsberger, N. Kampleitner, A.V. Grasse, L. Lindorfer, J. Robb, F. Beikzadeh, F. Strahodinsky, H. Leitner, H. Rajendran, T. Schmitt, S. Cremer, Nature Communications 16 (2025).","chicago":"Dawson, Erika, Michaela Hönigsberger, Niklas Kampleitner, Anna V Grasse, Lukas Lindorfer, Jennifer Robb, Farnaz Beikzadeh, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>.","ista":"Dawson E, Hönigsberger M, Kampleitner N, Grasse AV, Lindorfer L, Robb J, Beikzadeh F, Strahodinsky F, Leitner H, Rajendran H, Schmitt T, Cremer S. 2025. Altruistic disease signalling in ant colonies. Nature Communications. 16, 10511."},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.02.27.582277"}],"file":[{"file_id":"20826","checksum":"06244623bb7611c636652ecbc4787889","success":1,"date_updated":"2025-12-15T13:30:33Z","file_name":"2025_NatureComm_Dawson.pdf","access_level":"open_access","relation":"main_file","date_created":"2025-12-15T13:30:33Z","content_type":"application/pdf","file_size":805323,"creator":"dernst"}],"publisher":"Springer Nature","department":[{"_id":"SyCr"},{"_id":"LifeSc"}],"external_id":{"pmid":["41330896"]},"corr_author":"1","publication_status":"published","pmid":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_number":"10511","author":[{"first_name":"Erika","last_name":"Dawson","full_name":"Dawson, Erika","id":"31B4E2D0-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hönigsberger","first_name":"Michaela","id":"953894f3-25bd-11ec-8556-f70a9d38ef60","full_name":"Hönigsberger, Michaela"},{"full_name":"Kampleitner, Niklas","id":"2AC57FAC-F248-11E8-B48F-1D18A9856A87","first_name":"Niklas","last_name":"Kampleitner"},{"full_name":"Grasse, Anna V","id":"406F989C-F248-11E8-B48F-1D18A9856A87","first_name":"Anna V","last_name":"Grasse"},{"first_name":"Lukas","last_name":"Lindorfer","full_name":"Lindorfer, Lukas","id":"85f0e6d3-06b3-11ec-8982-8c5049fa4455"},{"last_name":"Robb","first_name":"Jennifer","id":"7bc2734a-e2c6-11ea-9824-a2ed5f0662a8","full_name":"Robb, Jennifer"},{"last_name":"Beikzadeh Abbasi","first_name":"Farnaz","id":"0344bfb9-3feb-11ee-87e9-c27edc800bcd","full_name":"Beikzadeh Abbasi, Farnaz"},{"last_name":"Strahodinsky","first_name":"Florian","id":"979E35EE-C996-11E9-8C7C-CF13E6697425","full_name":"Strahodinsky, Florian"},{"last_name":"Leitner","first_name":"Hanna","id":"8fc5c6f6-5903-11ec-abad-c83f046253e7","full_name":"Leitner, Hanna"},{"full_name":"Rajendran, Harikrishnan","id":"876b6b34-8ff4-11ec-97c9-8d95a7aae416","first_name":"Harikrishnan","last_name":"Rajendran"},{"last_name":"Schmitt","first_name":"Thomas","full_name":"Schmitt, Thomas"},{"last_name":"Cremer","first_name":"Sylvia","id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2193-3868","full_name":"Cremer, Sylvia"}],"DOAJ_listed":"1","PlanS_conform":"1","project":[{"_id":"2649B4DE-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"771402","name":"Epidemics in ant societies on a chip"},{"_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854","name":"IST Austria Open Access Fund"}],"abstract":[{"text":"Sick individuals often conceal their disease status to group members, thereby preventing social exclusion or aggression. Here we show by behavioural, chemical, immunological and infection load analyses that sick ant pupae instead actively emit a chemical signal that in itself is sufficient to trigger their own destruction by colony members. In our experiments, this altruistic disease-signalling was performed only by worker but not queen pupae. The lack of signalling by queen pupae did not constitute cheating behaviour, but reflected their superior immune capabilities. Worker pupae suffered from extensive pathogen replication whereas queen pupae were able to restrain their infection. Our data suggest the evolution of a finely-tuned signalling system in which it is not the induction of an individual’s immune response, but rather its failure to overcome the infection, that triggers pupal signalling for sacrifice. This demonstrates a balanced interplay between individual and social immunity that efficiently achieves whole-colony health.","lang":"eng"}],"month":"12","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"MassSpec"}],"APC_amount":"7068 EUR","volume":16,"date_published":"2025-12-01T00:00:00Z","file_date_updated":"2025-12-15T13:30:33Z","year":"2025","article_type":"original","date_created":"2025-01-27T11:28:05Z","acknowledgement":"We thank Joergen Eilenberg and Nicolai V. Meyling for the fungal strain, and the ISTA Social Immunity team, Jonghyun Park and Yuko Ulrich for ant collection. We also thank the Social Immunity team, in particular David Moreno Martínez, Tanvi Madaan, Wilfrid Jean Louis and Jessica Kirchner, for experimental and molecular support, as well as Friedrich Fochler for technical support with the chemical analysis, and the ISTA Lab Support Facility, including the mass spectrometry unit, for general and chemical laboratory support. We further thank Marco Ribezzi for advice on 13C calculations and Ernst Pittenauer for discussion of the chemical data, Chris Pull and Michael Sixt for project discussion, and the Social Immunity team for comments on the manuscript. The study was funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation Programme (No. 771402; EPIDEMICSonCHIP) to SC.","language":[{"iso":"eng"}],"oa":1,"intvolume":"        16"}]