[{"publication_status":"published","license":"https://creativecommons.org/licenses/by/4.0/","_id":"21384","scopus_import":"1","acknowledgement":"N.S.G. is the incumbent of the Lee and William Abramowitz Professorial Chair of Biophysics (Weizmann Institute), and acknowledges support from the Royal Society Wolfson Visiting Fellowship, and Human Frontier Science Program grant RGP0032/2022. Work by M.S., I.W., G.R. and A.G. was supported by the Leverhulme Trust (grant RPG-2021-226) and the European Research Council (ERC) under the Horizon 2020 program and UKRI, Grant agreement No.\r\nEP/Y02799X/1. M.S. and I.d.V acknowledge support by the European Research Council (grant ERC-SyG 101071793 to M.S). The funders had no role in study design, data collection and\r\nanalysis, decision to publish, or preparation of the manuscript.","author":[{"last_name":"Liu","first_name":"Jiayi","full_name":"Liu, Jiayi"},{"full_name":"Ron, Jonathan E.","last_name":"Ron","first_name":"Jonathan E."},{"full_name":"Rinaldi, Giulia","first_name":"Giulia","last_name":"Rinaldi"},{"first_name":"Ivanna","last_name":"Williantarra","full_name":"Williantarra, Ivanna"},{"last_name":"Georgantzoglou","first_name":"Antonios","full_name":"Georgantzoglou, Antonios"},{"full_name":"de Vries, Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","last_name":"de Vries"},{"orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"},{"full_name":"Sarris, Milka","last_name":"Sarris","first_name":"Milka"},{"full_name":"Gov, Nir S.","first_name":"Nir S.","last_name":"Gov"}],"article_type":"original","has_accepted_license":"1","publisher":"Public Library of Science","abstract":[{"lang":"eng","text":"Cell migration in vivo is often guided by chemical signaling, i.e., chemotaxis. For immune cells performing chemotaxis in the organism, this process is influenced by the complex geometry of the tissue environment. In this study, we use a theoretical model of branched cell migration on a network to explore the cellular response to chemical gradients. The model predicts the response of a branched cell to a chemical gradient: how the cell reorients its internal polarity and how it navigates through a complex environment up a chemical gradient. We then compare the model’s predictions with experimental observations of neutrophils migrating to the site of a laser-inflicted wound in a zebrafish larva fin, and neutrophils migrating in vitro inside a regular lattice of pillars. We find that the model captures the details of the subcellular response to the chemokine gradient, as well as qualitative characteristics of the large-scale migration, suggesting that the neutrophils behave as fast cells, which explains the functionality of these immune cells."}],"title":"Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation","OA_place":"publisher","fulldoi":"https://doi.org/10.1371/journal.pcbi.1013934","article_number":"e1013934","ddc":["570"],"type":"journal_article","article_processing_charge":"Yes","doi":"10.1371/journal.pcbi.1013934","day":"03","project":[{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793"}],"quality_controlled":"1","date_updated":"2026-03-02T14:12:22Z","oa":1,"date_published":"2026-02-03T00:00:00Z","month":"02","citation":{"short":"J. Liu, J.E. Ron, G. Rinaldi, I. Williantarra, A. Georgantzoglou, I. de Vries, M.K. Sixt, M. Sarris, N.S. Gov, PLOS Computational Biology 22 (2026).","ieee":"J. Liu <i>et al.</i>, “Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation,” <i>PLOS Computational Biology</i>, vol. 22, no. 2. Public Library of Science, 2026.","ista":"Liu J, Ron JE, Rinaldi G, Williantarra I, Georgantzoglou A, de Vries I, Sixt MK, Sarris M, Gov NS. 2026. Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation. PLOS Computational Biology. 22(2), e1013934.","chicago":"Liu, Jiayi, Jonathan E. Ron, Giulia Rinaldi, Ivanna Williantarra, Antonios Georgantzoglou, Ingrid de Vries, Michael K Sixt, Milka Sarris, and Nir S. Gov. “Modelling Chemotaxis of Branched Cells in Complex Environments Provides Insights into Immune Cell Navigation.” <i>PLOS Computational Biology</i>. Public Library of Science, 2026. <a href=\"https://doi.org/10.1371/journal.pcbi.1013934\">https://doi.org/10.1371/journal.pcbi.1013934</a>.","mla":"Liu, Jiayi, et al. “Modelling Chemotaxis of Branched Cells in Complex Environments Provides Insights into Immune Cell Navigation.” <i>PLOS Computational Biology</i>, vol. 22, no. 2, e1013934, Public Library of Science, 2026, doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1013934\">10.1371/journal.pcbi.1013934</a>.","apa":"Liu, J., Ron, J. E., Rinaldi, G., Williantarra, I., Georgantzoglou, A., de Vries, I., … Gov, N. S. (2026). Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation. <i>PLOS Computational Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pcbi.1013934\">https://doi.org/10.1371/journal.pcbi.1013934</a>","ama":"Liu J, Ron JE, Rinaldi G, et al. Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation. <i>PLOS Computational Biology</i>. 2026;22(2). doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1013934\">10.1371/journal.pcbi.1013934</a>"},"department":[{"_id":"MiSi"}],"date_created":"2026-03-02T10:08:38Z","oa_version":"Published Version","volume":22,"OA_type":"gold","issue":"2","DOAJ_listed":"1","year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"PLOS Computational Biology","pmid":1,"external_id":{"pmid":["41632822"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"language":[{"iso":"eng"}],"file":[{"success":1,"file_name":"2026_PloSCompBio_.pdf","content_type":"application/pdf","date_created":"2026-03-02T14:11:14Z","access_level":"open_access","checksum":"564041089e7334804ad3cade973f80b4","file_size":20688452,"file_id":"21388","date_updated":"2026-03-02T14:11:14Z","creator":"dernst","relation":"main_file"}],"PlanS_conform":"1","intvolume":"        22","publication_identifier":{"eissn":["1553-7358"]},"status":"public","file_date_updated":"2026-03-02T14:11:14Z"},{"publication_status":"epub_ahead","author":[{"last_name":"Dehio","first_name":"Philippe G","id":"b769738e-a003-11ee-b1b8-9030316e0d59","full_name":"Dehio, Philippe G"},{"last_name":"Michard","first_name":"Céline","full_name":"Michard, Céline"},{"last_name":"Yam-Puc","first_name":"Juan Carlos","full_name":"Yam-Puc, Juan Carlos"},{"full_name":"Martí I Líndez, Adrià Arnau","first_name":"Adrià Arnau","last_name":"Martí I Líndez"},{"full_name":"Jandke, Anett","last_name":"Jandke","first_name":"Anett"},{"full_name":"Unterstab, Gunhild","first_name":"Gunhild","last_name":"Unterstab"},{"last_name":"Fabre","first_name":"Lucien","full_name":"Fabre, Lucien"},{"full_name":"Sauteur, Loïc","first_name":"Loïc","last_name":"Sauteur"},{"last_name":"Artinger","first_name":"Marc","full_name":"Artinger, Marc"},{"full_name":"Legler, Daniel F.","last_name":"Legler","first_name":"Daniel F."},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K"},{"last_name":"Schaefer","first_name":"Thorsten","full_name":"Schaefer, Thorsten"},{"first_name":"Matthias P.","last_name":"Wymann","full_name":"Wymann, Matthias P."},{"first_name":"Klaus","last_name":"Okkenhaug","full_name":"Okkenhaug, Klaus"},{"first_name":"Thierry","last_name":"Soldati","full_name":"Soldati, Thierry"},{"first_name":"Matthias","last_name":"Mehling","orcid":"0000-0001-8599-1226","full_name":"Mehling, Matthias","id":"3C23B994-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Christoph","last_name":"Hess","full_name":"Hess, Christoph"}],"acknowledgement":"We thank the microscopy core facility of the Department of Biomedicine at the University and University Hospital of Basel for their technical support. This research was technically supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). CH was supported by the Swiss National Science Foundation (SNSF) (310030B_201277; 310030_192677; FZEB-0-180487), the ZBF Program Award 2025 (Hans Zäslin Bustany Foundation), and the Novartis Foundation for Medical-Biological Research (NFMBR) (#23A070). PD was supported by the Swiss Academy for Medical Sciences (SAMW) and SNSF (183980, 225441), the NFMBR (#23A070), AlumniMedizin Basel, and the Freiwillige Akademische Gesellschaft Basel. DFL was supported by the SNSF (220205). Open access funding provided by University of Basel.","scopus_import":"1","_id":"22371","type":"journal_article","OA_place":"publisher","fulldoi":"https://doi.org/10.1038/s44319-026-00861-x","abstract":[{"lang":"eng","text":"Amoeboid cell migration is key to efficient T cell immunity. Spatial polarization of organelles within cells, including endo-lysosomes, is a prerequisite of migration. However, how ultrastructural polarization is linked to the signaling requirements governing T cell migration remains unknown. Here we show that signaling molecules generated by endo-lysosome-localized kinases regulate velocity of amoeboid migration. Specifically, imaging of T cells identifies accumulation of endo-lysosomes decorated with the lipid kinases VPS34–PIKfyve at the uropod of polarized cells. Activity of VPS34 and PIKfyve regulates speed, but not directedness, of migrating T cells. Mechanistically, PI(3,5)P2 generated by the sequential action of VPS34 and PIKfyve, mediates Ca2+ efflux from lysosomes via the mucolipin TRP cation channel 1 (TRPML1), thus controlling activity of myosin IIA and hence the generation of propulsive force through retrograde actin flow. The VPS34–PIKfyve kinases also regulate velocity of myeloid cells, as well as of the amoeba Dictyostelium discoideum – establishing the axis as an evolutionarily conserved speed control system of amoeboid cell migration."}],"publisher":"Springer Nature","title":"A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration","article_type":"original","article_processing_charge":"Yes (via OA deal)","month":"07","date_published":"2026-07-07T00:00:00Z","oa":1,"date_updated":"2026-07-20T14:28:59Z","quality_controlled":"1","day":"07","doi":"10.1038/s44319-026-00861-x","OA_type":"gold","oa_version":"Published Version","researchdata_availability":"yes","date_created":"2026-07-19T22:01:48Z","department":[{"_id":"MiSi"}],"main_file_link":[{"url":"https://doi.org/10.1038/s44319-026-00861-x","open_access":"1"}],"das_tickbox":"1","citation":{"ista":"Dehio PG, Michard C, Yam-Puc JC, Martí I Líndez AA, Jandke A, Unterstab G, Fabre L, Sauteur L, Artinger M, Legler DF, Sixt MK, Schaefer T, Wymann MP, Okkenhaug K, Soldati T, Mehling M, Hess C. 2026. A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration. EMBO Reports.","ieee":"P. G. Dehio <i>et al.</i>, “A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration,” <i>EMBO Reports</i>. Springer Nature, 2026.","short":"P.G. Dehio, C. Michard, J.C. Yam-Puc, A.A. Martí I Líndez, A. Jandke, G. Unterstab, L. Fabre, L. Sauteur, M. Artinger, D.F. Legler, M.K. Sixt, T. Schaefer, M.P. Wymann, K. Okkenhaug, T. Soldati, M. Mehling, C. Hess, EMBO Reports (2026).","ama":"Dehio PG, Michard C, Yam-Puc JC, et al. A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration. <i>EMBO Reports</i>. 2026. doi:<a href=\"https://doi.org/10.1038/s44319-026-00861-x\">10.1038/s44319-026-00861-x</a>","apa":"Dehio, P. G., Michard, C., Yam-Puc, J. C., Martí I Líndez, A. A., Jandke, A., Unterstab, G., … Hess, C. (2026). A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration. <i>EMBO Reports</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s44319-026-00861-x\">https://doi.org/10.1038/s44319-026-00861-x</a>","mla":"Dehio, Philippe G., et al. “A Conserved VPS34-PIKfyve-TRPML1-Myosin II Axis Regulates the Speed of Amoeboid Cell Migration.” <i>EMBO Reports</i>, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s44319-026-00861-x\">10.1038/s44319-026-00861-x</a>.","chicago":"Dehio, Philippe G, Céline Michard, Juan Carlos Yam-Puc, Adrià Arnau Martí I Líndez, Anett Jandke, Gunhild Unterstab, Lucien Fabre, et al. “A Conserved VPS34-PIKfyve-TRPML1-Myosin II Axis Regulates the Speed of Amoeboid Cell Migration.” <i>EMBO Reports</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s44319-026-00861-x\">https://doi.org/10.1038/s44319-026-00861-x</a>."},"external_id":{"pmid":["42414599"]},"pmid":1,"supplementarymaterial":"yes","publication":"EMBO Reports","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","dataavailabilitystatement":"The analysis workflow to quantify vesicle localization can be accessed on GitHub (https://github.com/loicsauteur/vesicle-analysis, version 0.1.1).\r\n\r\nThe source data of this paper are collected in the following database record: biostudies:S-SCDT-10_1038-S44319-026-00861-x.","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"year":"2026","DOAJ_listed":"1","status":"public","publication_identifier":{"eissn":["1469-3178"]},"language":[{"iso":"eng"}]},{"doi":"10.1038/s41467-026-72845-3","day":"04","quality_controlled":"1","date_updated":"2026-09-15T12:58:06Z","oa":1,"date_published":"2026-09-04T00:00:00Z","month":"09","citation":{"apa":"Strauss, L., Lembo, S., Gérard, S. F., Siggel, M., Cheng, D., Bergert, M., … Diz-Muñoz, A. (2026). The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-72845-3\">https://doi.org/10.1038/s41467-026-72845-3</a>","mla":"Strauss, Léanne, et al. “The Membrane-to-Cortex Distance Regulates MDia1 Activity to Control Cortical Mechanics.” <i>Nature Communications</i>, vol. 17, 9501, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-72845-3\">10.1038/s41467-026-72845-3</a>.","chicago":"Strauss, Léanne, Sergio Lembo, Samuel F. Gérard, Marc Siggel, Dorothy Cheng, Martin Bergert, Sarah K. Foster, et al. “The Membrane-to-Cortex Distance Regulates MDia1 Activity to Control Cortical Mechanics.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-72845-3\">https://doi.org/10.1038/s41467-026-72845-3</a>.","ama":"Strauss L, Lembo S, Gérard SF, et al. The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-72845-3\">10.1038/s41467-026-72845-3</a>","ieee":"L. Strauss <i>et al.</i>, “The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026.","short":"L. Strauss, S. Lembo, S.F. Gérard, M. Siggel, D. Cheng, M. Bergert, S.K. Foster, J. Vermeil, M. Toro-Nahuelpan, L.M. Fischer, Q. Yu, E. Sitarska, C.J. Chan, J. Kosinski, M. Piel, O. Du Roure, J. Heuvingh, J. Mahamid, A. Diz-Muñoz, Nature Communications 17 (2026).","ista":"Strauss L, Lembo S, Gérard SF, Siggel M, Cheng D, Bergert M, Foster SK, Vermeil J, Toro-Nahuelpan M, Fischer LM, Yu Q, Sitarska E, Chan CJ, Kosinski J, Piel M, Du Roure O, Heuvingh J, Mahamid J, Diz-Muñoz A. 2026. The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics. Nature Communications. 17, 9501."},"das_tickbox":"1","department":[{"_id":"MiSi"}],"date_created":"2026-09-13T22:01:51Z","researchdata_availability":"yes","oa_version":"Published Version","OA_type":"gold","volume":17,"DOAJ_listed":"1","year":"2026","dataavailabilitystatement":"The dataset for the analysis of cortical actin and p-myosin in fixed cells, as well as actin and myosin−2 in live cells can be found together with the corresponding Fiji macro for immunofluorescence quantification in the Biostudies database (accession number S-BIAD2611).\r\n\r\nAll raw frames, tilt series, metadata, tomograms and segmentations generated for this work are deposited in the Electron Microscopy Public Image Archive (EMPIAR)118 under accession code EMPIAR-13326. A representative tomogram associated with this entry is available in the Electron Microscopy Data Bank (EMDB)119 under entry EMD-56367.\r\n\r\nThe experimental imaging data for the filopodia analysis, the used Spotiflow model with corresponding training data and example datasets are available in the BioStudies database (accession number S-BIAD2611).\r\n\r\nRaw numbers for plots presented in this paper as well as western blot images are available in the Source Data. All other data and unique reagents that support this study are available from the corresponding authors upon request. Source data are provided in this paper. Source data are provided with this paper.\r\nThe code used for cortex analysis from binary segmentations obtained during cryo-ET data processing is available on GitHub with the following link: https://github.com/MahamidLab/actin_cortex_analysis/tree/revision.\r\n\r\nThe Napari plugin for reviewing and manually correct spot detections used in the filopodia analysis is available at GitHub with the following link: https://github.com/diz-lab/filospot.","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","supplementarymaterial":"yes","publication":"Nature Communications","pmid":1,"external_id":{"pmid":["42697882"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"language":[{"iso":"eng"}],"file":[{"success":1,"file_name":"2026_NatureComm_Strauss.pdf","content_type":"application/pdf","date_created":"2026-09-15T12:51:36Z","access_level":"open_access","checksum":"adc0d16fcb2a65e1a5913481700fa19f","file_size":3154967,"file_id":"22932","date_updated":"2026-09-15T12:51:36Z","creator":"dernst","relation":"main_file"}],"PlanS_conform":"1","corr_author":"1","intvolume":"        17","publication_identifier":{"eissn":["2041-1723"]},"status":"public","file_date_updated":"2026-09-15T12:51:36Z","publication_status":"published","_id":"22911","scopus_import":"1","acknowledgement":"We thank Jan Ellenberg (SciLifeLab), Stephan Grill (MPI-CBG), Anna Erzberger (EMBL) and members of the Diz-Muñoz lab for a critical reading of the manuscript. We thank Estela Sosa Osorio (EMBL) and Gisela Juliachs Torroella (EMBL) for manual curation of actin segmentation in cryo-electron tomograms, and Evgenia Zagoriy (EMBL) and Mukthi Ammai Sridharan Iyer (EMBL) for the visual inspection of actin branching points in cryo-electron tomograms. We thank Anne-Cecyle Reyman (IGBMC) and her team for extended discussion and inspiring experiments. We thank Ruben Tesoro Moreno (EMBL) for Alphafold predictions. We thank Jan Faix (Hannover Medical School) for the mDia1 constructs and Jamie Hackett (EMBL Rome) for the Piggybac vectors. We thank Sarah Kaspar in the EMBL Data Science Centre for help with statistical methods. We thank the EMBL Flow Cytometry Core Facility (especially Daniel Gimenes), the EMBL advanced light microscopy facility (especially Marko Lampe and Beate Neumann for microscope support, and Christian Tischer for help with image analysis), EMBL IT (especially Thomas Hoffmann), and the EMBL cryo-EM platform for support and advice. We thank Albert Dominguez Mantes (EPFL) for helpful discussions about Spotiflow. We acknowledge the financial support of the European Molecular Biology Laboratory (EMBL) to J.M. and A.D-M., the Deutsche Forschungsgemeinschaft (DFG) grant DI 2205/3-1, the Human Frontiers Science Program (HFSP) grant RGY0073/2018 and ERC grant 101124221 (MitoMeChAnics) to A.D-M., the Boehringer Ingelheim Fonds PhD fellowship and the Croucher Scholarship for Doctoral Study to D.C., the EMBL interdisciplinary Postdoc (EIPOD) programme under Marie Curie Cofund Actions MSCA-COFUND-FP to M.S. and M.T-N., the EMBO fellowship to S.F.G., the French Agence Nationale de la Recherche (ANR-21-CE13-0048) to O.D.R. and J.H.; This work is partially funded by the European Union. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council Executive Agency. Neither the European Union nor the granting authority can be held responsible for them. Open Access funding enabled and organized by Projekt DEAL.","author":[{"full_name":"Strauss, Léanne","last_name":"Strauss","first_name":"Léanne"},{"full_name":"Lembo, Sergio","id":"d993a7b2-292f-11ed-aaac-fb045a912e31","last_name":"Lembo","first_name":"Sergio","orcid":"0000-0002-2253-8771"},{"first_name":"Samuel F.","last_name":"Gérard","full_name":"Gérard, Samuel F."},{"last_name":"Siggel","first_name":"Marc","full_name":"Siggel, Marc"},{"full_name":"Cheng, Dorothy","last_name":"Cheng","first_name":"Dorothy"},{"last_name":"Bergert","first_name":"Martin","full_name":"Bergert, Martin"},{"full_name":"Foster, Sarah K.","last_name":"Foster","first_name":"Sarah K."},{"last_name":"Vermeil","first_name":"Joseph","full_name":"Vermeil, Joseph"},{"first_name":"Mauricio","last_name":"Toro-Nahuelpan","full_name":"Toro-Nahuelpan, Mauricio"},{"full_name":"Fischer, Lena M.","first_name":"Lena M.","last_name":"Fischer"},{"first_name":"Qin","last_name":"Yu","full_name":"Yu, Qin"},{"full_name":"Sitarska, Ewa","first_name":"Ewa","last_name":"Sitarska"},{"full_name":"Chan, Chii Jou","last_name":"Chan","first_name":"Chii Jou"},{"last_name":"Kosinski","first_name":"Jan","full_name":"Kosinski, Jan"},{"last_name":"Piel","first_name":"Matthieu","full_name":"Piel, Matthieu"},{"last_name":"Du Roure","first_name":"Olivia","full_name":"Du Roure, Olivia"},{"full_name":"Heuvingh, Julien","first_name":"Julien","last_name":"Heuvingh"},{"full_name":"Mahamid, Julia","first_name":"Julia","last_name":"Mahamid"},{"last_name":"Diz-Muñoz","first_name":"Alba","full_name":"Diz-Muñoz, Alba"}],"has_accepted_license":"1","article_type":"original","abstract":[{"lang":"eng","text":"The shape of animal cells is controlled by their surface, which comprises the cell cortex, a peripheral actin network, tethered to the plasma membrane by membrane-to-cortex attachment proteins. Changes in cortical components have long been considered to dominate the regulation of forces and mechanical properties at the cell surface and drive morphogenesis. Here, we show that the coupling of the cortex to the membrane is also key for the regulation of its mechanical properties. By combining molecular engineering with biophysical approaches and in-cell cryo-electron tomography we describe the cell surface with nanometer-resolution and link its organization to cell-scale mechanics. We find that membrane-to-cortex attachment proteins can physically draw the cortex closer to the membrane, in a density and length-dependent manner. This reduction of the membrane-to-cortex distance controls the activity of the formin mDia1, leading to a reduction in cortical tension. Our study thus defines a novel mechanism whereby the membrane-to-cortex distance is a functional geometrical parameter that regulates cell surface properties."}],"title":"The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics","publisher":"Springer Nature","fulldoi":"https://doi.org/10.1038/s41467-026-72845-3","OA_place":"publisher","article_number":"9501","ddc":["570"],"type":"journal_article","article_processing_charge":"Yes (via OA deal)"},{"fulldoi":"https://doi.org/10.1126/sciadv.aed0587","OA_place":"publisher","type":"journal_article","ddc":["570"],"article_number":"eaed0587","publisher":"AAAS","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening","abstract":[{"lang":"eng","text":"Although studies of tumor invasion have mainly emphasized the evolution of genetic and epigenetic heterogeneity, cellular behaviors also depend on the physical microenvironment. Yet, the tumor microenvironment is both complex and continuously remodeled, making it hard to rigorously test its causal influence on tumor dissemination. Here, we adopted a reductionist approach to studying tumor cell invasion in microengineered environments of tunable patterned geometries. We find that not only the presence of obstacles but more unexpectedly their spatial disorder causes a drastic shift from a collective to a single-cell mode of invasion—comparable in strength to loss of cell-cell adhesion. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell dissemination results both from local geometrical constraints and a global integration of spatial disorder over time. We identify generic universality classes of invasion dynamics dependent on environmental geometry, enabling us to identify quantitative physical principles for tumor dissemination."}],"has_accepted_license":"1","article_type":"original","article_processing_charge":"Yes","publication_status":"published","author":[{"last_name":"Dunajova","first_name":"Zuzana","id":"4B39F286-F248-11E8-B48F-1D18A9856A87","full_name":"Dunajova, Zuzana"},{"full_name":"Tasciyan, Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","first_name":"Saren","last_name":"Tasciyan","orcid":"0000-0003-1671-393X"},{"last_name":"Majek","first_name":"Juraj","full_name":"Majek, Juraj","id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1"},{"first_name":"Jack","last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Jenkins, Robert P.","last_name":"Jenkins","first_name":"Robert P."},{"first_name":"Erik","last_name":"Sahai","full_name":"Sahai, Erik"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo"}],"_id":"22963","scopus_import":"1","acknowledgement":"We thank all the members of the Hannezo and Sixt groups for fruitful discussions and t. Kato for advice on the experimental setup. We are also thankful for support from the Scientific Service Units (SSU) of IST Austria through resources provided by the imaging and Optics Facility (iOF) and the Lab Support Facility (lSF). We used chatGPt (GPt- 5,OpenAi, 2025) to make minor grammatical and stylistic revisions to the original manuscript, which was written by us. We carefully reviewed and verified all changes and are solely responsible for all scientific content, data, interpretation, and conclusions. Funding: Z.D. has received funding from the Doctoral Programme of the Austrian Academy of Sciences (OeAW):grant agreement 26360. this work was supported by the European Research council (grant ERC- SyG 101071793 to M.S.). E.S. and R.P.J. are supported by the Francis Crick Institute, which receives its core funding from cancer Research UK (cc2040), the UK Medical Research council(cc2040), and the Wellcome trust (cc2040) and the European Research council (ERC Advanced Grant cAn_ORGAniSe, grant agreement number 101019366).","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","supplementarymaterial":"no","publication":"Science Advances","external_id":{"pmid":["42715298"]},"DOAJ_listed":"1","dataavailabilitystatement":" All data and code needed to evaluate and reproduce the results in the paper are present in the paper and/or the Supplementary Materials and the associated data and code repositories. Source data underlying all figures and supplementary figures have been deposited in a Zenodo repository (DOi: 10.5281/zenodo.21718217) and will be made publicly available upon publication. custom simulation and analysis codes used in this study are available at https://codeocean.com/capsule/1237446/tree. no unique biological materials were generated in this study. Microfluidic mask designs generated for this work are available from the corresponding authors upon reasonable request.","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"year":"2026","related_material":{"record":[{"status":"public","relation":"earlier_version","id":"21427"}]},"intvolume":"        12","language":[{"iso":"eng"}],"PlanS_conform":"1","corr_author":"1","file":[{"checksum":"79f419b659650a39b3dfa58c782690a8","date_created":"2026-10-06T11:30:48Z","access_level":"open_access","content_type":"application/pdf","file_name":"2026_ScienceAdv_Dunajova.pdf","success":1,"relation":"main_file","file_id":"23062","creator":"dernst","date_updated":"2026-10-06T11:30:48Z","file_size":11691064}],"status":"public","file_date_updated":"2026-10-06T11:30:48Z","publication_identifier":{"eissn":["2375-2548"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"oa":1,"date_updated":"2026-10-06T11:36:34Z","quality_controlled":"1","month":"09","date_published":"2026-09-09T00:00:00Z","doi":"10.1126/sciadv.aed0587","project":[{"_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","name":"Motile active matter models of migrating cells and chiral filaments","grant_number":"26360"},{"grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"}],"day":"09","issue":"37","OA_type":"gold","volume":12,"researchdata_availability":"yes","oa_version":"Published Version","das_tickbox":"1","citation":{"ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Jenkins RP, Sahai E, Sixt MK, Hannezo EB. 2026. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. Science Advances. 12(37), eaed0587.","short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, R.P. Jenkins, E. Sahai, M.K. Sixt, E.B. Hannezo, Science Advances 12 (2026).","ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening,” <i>Science Advances</i>, vol. 12, no. 37. AAAS, 2026.","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>Science Advances</i>. 2026;12(37). doi:<a href=\"https://doi.org/10.1126/sciadv.aed0587\">10.1126/sciadv.aed0587</a>","chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Robert P. Jenkins, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>Science Advances</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/sciadv.aed0587\">https://doi.org/10.1126/sciadv.aed0587</a>.","mla":"Dunajova, Zuzana, et al. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>Science Advances</i>, vol. 12, no. 37, eaed0587, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aed0587\">10.1126/sciadv.aed0587</a>.","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Jenkins, R. P., Sahai, E., … Hannezo, E. B. (2026). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.aed0587\">https://doi.org/10.1126/sciadv.aed0587</a>"},"date_created":"2026-09-20T22:01:48Z","department":[{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"GradSch"},{"_id":"AnSa"},{"_id":"NanoFab"}]},{"researchdata_availability":"yes","oa_version":"Published Version","OA_type":"hybrid","department":[{"_id":"MiSi"},{"_id":"Bio"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41586-026-11063-9"}],"date_created":"2026-10-19T09:26:53Z","das_tickbox":"1","citation":{"ista":"Radler P, Viehboeck T, Luo Z-H, Maslać N, Schmidt K, Hauschild R, Nobu MK, Bulgheresi S, Stradal TEB, Rottner K, Imachi H, Sixt MK, Schleper C. 2026. Dynamic protrusions mediate crawling motility in Asgard archaea. Nature.","short":"P. Radler, T. Viehboeck, Z.-H. Luo, N. Maslać, K. Schmidt, R. Hauschild, M.K. Nobu, S. Bulgheresi, T.E.B. Stradal, K. Rottner, H. Imachi, M.K. Sixt, C. Schleper, Nature (2026).","ieee":"P. Radler <i>et al.</i>, “Dynamic protrusions mediate crawling motility in Asgard archaea,” <i>Nature</i>. Springer Nature, 2026.","ama":"Radler P, Viehboeck T, Luo Z-H, et al. Dynamic protrusions mediate crawling motility in Asgard archaea. <i>Nature</i>. 2026. doi:<a href=\"https://doi.org/10.1038/s41586-026-11063-9\">10.1038/s41586-026-11063-9</a>","chicago":"Radler, Philipp, Tobias Viehboeck, Zhen-Hao Luo, Nevena Maslać, Katharina Schmidt, Robert Hauschild, Masaru K. Nobu, et al. “Dynamic Protrusions Mediate Crawling Motility in Asgard Archaea.” <i>Nature</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41586-026-11063-9\">https://doi.org/10.1038/s41586-026-11063-9</a>.","mla":"Radler, Philipp, et al. “Dynamic Protrusions Mediate Crawling Motility in Asgard Archaea.” <i>Nature</i>, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41586-026-11063-9\">10.1038/s41586-026-11063-9</a>.","apa":"Radler, P., Viehboeck, T., Luo, Z.-H., Maslać, N., Schmidt, K., Hauschild, R., … Schleper, C. (2026). Dynamic protrusions mediate crawling motility in Asgard archaea. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-026-11063-9\">https://doi.org/10.1038/s41586-026-11063-9</a>"},"date_published":"2026-09-30T00:00:00Z","month":"09","quality_controlled":"1","oa":1,"date_updated":"2026-10-19T09:40:50Z","day":"30","project":[{"grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"},{"_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy","grant_number":"CZI01"}],"doi":"10.1038/s41586-026-11063-9","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"status":"public","PlanS_conform":"1","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"external_id":{"pmid":["42816618"]},"publication":"Nature","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","supplementarymaterial":"yes","pmid":1,"year":"2026","acknowledged_ssus":[{"_id":"Bio"}],"dataavailabilitystatement":"The plots in this manuscript were created using Jupyter Notebook; the source data for all figures have been provided in data source tables. Microscopy raw data for supplementary videos, the Jupyter notebooks to generate the plots and the raw files for the structural phylogenetic analysis are available at https://doi.org/10.6084/m9.figshare.30676367. The remaining raw microscopy data generated for this manuscript are available from https://www.ebi.ac.uk/biostudies/bioimages/studies/S-BIAD3928. The mass spectrometry proteomics data from the Co-IP experiments have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository79 and are available at https://www.ebi.ac.uk/pride/archive/projects/PXD068820. The mass spectrometry proteomics data of the proteome analysis is available at https://www.ebi.ac.uk/pride/archive/projects/PXD074767. Source data are provided with this paper.","author":[{"last_name":"Radler","first_name":"Philipp","orcid":"0000-0001-9198-2182 ","full_name":"Radler, Philipp","id":"40136C2A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Viehboeck, Tobias","first_name":"Tobias","last_name":"Viehboeck"},{"full_name":"Luo, Zhen-Hao","last_name":"Luo","first_name":"Zhen-Hao"},{"first_name":"Nevena","last_name":"Maslać","full_name":"Maslać, Nevena"},{"last_name":"Schmidt","first_name":"Katharina","full_name":"Schmidt, Katharina"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","last_name":"Hauschild"},{"last_name":"Nobu","first_name":"Masaru K.","full_name":"Nobu, Masaru K."},{"last_name":"Bulgheresi","first_name":"Silvia","full_name":"Bulgheresi, Silvia"},{"first_name":"Theresia E. B.","last_name":"Stradal","full_name":"Stradal, Theresia E. B."},{"last_name":"Rottner","first_name":"Klemens","full_name":"Rottner, Klemens"},{"full_name":"Imachi, Hiroyuki","first_name":"Hiroyuki","last_name":"Imachi"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"},{"full_name":"Schleper, Christa","last_name":"Schleper","first_name":"Christa"}],"acknowledgement":"This work would not have been possible without the technical support in cultivation from K. Hager, F. Baraket, A. Malits and S. Harrer. We thank the Imaging and Optics Facility (IOF) at ISTA for their help in setting up the anaerobic environment during imaging and the Cell Imaging & Ultrastructure Research (CIUS) facility at the University of Vienna for their support. Proteomics analyses were performed by the Mass Spectrometry Facility at Max Perutz Labs using the VBCF instrument pool. We also thank P. Klahn (University of Gothenburg, Sweden), M. Stadler and members of his department (HZI Braunschweig) as well as R. Cox (LUH Hannover) for providing different cytochalasans and P. Bieling (King’s College London) for discussion. Most of this work was funded by the Austrian Science Fund (FWF) with grant DOI 10.55776/Z437 (available at https://doi.org/10.55776/Z437) and further supported by an emerging field grant with grant DOI 10.55776/EFP25 (available at https://doi.org/10.55776/EFP25) to C.S. For open access purposes, the author has applied a CC BY public copyright license to any author accepted manuscript version arising from this submission. This work was further supported by a European Research Council grant ERC-SyG (101071793) to M.S., by the German Research Council through the CytoLabs consortium (Research Unit 5170 to K.R. and T.E.B.S.), by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research 22H04985 to H.I. and M.N. Furthermore, R.H. was supported by CZI grant DAF2020-225401 and a grant (https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF. Open access funding provided by University of Vienna.","scopus_import":"1","_id":"23171","publication_status":"epub_ahead","article_processing_charge":"Yes (via OA deal)","ddc":["570"],"type":"journal_article","OA_place":"publisher","fulldoi":"https://doi.org/10.1038/s41586-026-11063-9","has_accepted_license":"1","article_type":"original","title":"Dynamic protrusions mediate crawling motility in Asgard archaea","abstract":[{"text":"Crawling motility is a hallmark of eukaryotic cells and requires a dynamic actin cytoskeleton, regulated adhesion and spatially organized signalling pathways1,2. Asgard archaea, which are considered the closest known prokaryotic relatives of eukaryotes, potentially encode these functions within their large set of ‘eukaryotic signature proteins’3,4,5,6. The few cultivated members show a complex cell morphology, consisting of a central cell body from which several protrusions extend, filled with an actin-based cytoskeleton7,8. Here, live-cell microscopy of two organisms of the Lokiarchaea and Hodarchaea lineages7,9 showed that they dynamically and greatly change their cell shape on a minute time scale and grow and retract their extensive protrusions with a speed of 1.5–4.8 µm min−1, respectively. After adhering to a glass surface, cells use their protrusions to undergo active crawling motion. In the presence of selected actin inhibitors, however, the observed dynamics were arrested, suggesting a central role of actin in these processes. The observed cellular plasticity and motility are unique features among prokaryotes and might have been crucial for the emergence of the first eukaryotic cells that are thought to have formed through the association of a member of the Asgard archaea and an α-proteobacterium, the ancestor of mitochondria.","lang":"eng"}],"publisher":"Springer Nature"},{"publication_status":"published","scopus_import":"1","_id":"21707","acknowledgement":"We thank I. Salecker (Flybow), B. Altenhein (Fas2-Gal4Mz507), A. Nose (UAS-intra- and extra-Fas2::YFP), and C. S. Goodman (UAS-Fas2PEST+/−), as well as the Bloomington Stock Center for providing materials and fly stocks. We thank S. Waddell and the lab, especially B. Senapati, for providing the opportunity to conduct memory experiments at the CNCB, University of Oxford, and for supervision and discussions during this period. We also thank W. Kallina, S. Ilgerl, D. Bartel, A. Grimm, and A. Litin for technical support and the Hummel Lab for stimulating discussions and critical comments on the manuscript. We acknowledge the early exploratory work of A. Mattia, S. Trkulja, C. Schönherr, S. Bogner, B. Simpson, L. Tomasek, H. Roth, H. Vokač, R. Gredler, F. Kapelari, T. Kolarova, C. Ignitsch, Á. Bautista-Soldevila, and M. Kassem.\r\nThis research was funded by the University of Vienna, the Vienna Doctoral School Cognition, Behaviour and Neuroscience (uni:docs fellowship) (to J.W.M.) and by the Austrian Science Fund (FWF) (Cluster of Excellence Neuronal Circuits in Health and Disease, grant DOI 10.55776/COE16; https://www.fwf.ac.at/en/research-radar/10.55776/COE16) (to T.H.). For open access purposes, the author has applied a CC BY public copyright license to any author-accepted manuscript version arising from this submission.","author":[{"first_name":"Johann W.","last_name":"Markovitsch","full_name":"Markovitsch, Johann W."},{"full_name":"Mitić, Daniel","last_name":"Mitić","first_name":"Daniel"},{"full_name":"Del Pilar Jiménez García, Alisa","last_name":"Del Pilar Jiménez García","first_name":"Alisa"},{"orcid":"0009-0003-0415-7603","first_name":"Alsberga","last_name":"Zane","id":"60f7509a-f652-11ea-9d86-b963d6490d7c","full_name":"Zane, Alsberga"},{"full_name":"Kainz, Sarah","last_name":"Kainz","first_name":"Sarah"},{"first_name":"Rashmit","last_name":"Kaur","full_name":"Kaur, Rashmit"},{"full_name":"Hummel, Thomas","last_name":"Hummel","first_name":"Thomas"}],"has_accepted_license":"1","article_type":"original","publisher":"AAAS","title":"Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity","abstract":[{"lang":"eng","text":"Structural and functional differences between brain hemispheres are a common feature of animal nervous systems with reduced bilateral asymmetry often linked to impaired cognitive performance. How neuronal left-right asymmetry is initiated and integrated into a bilaterally symmetrical ground pattern is poorly understood. Here, we show that the directional asymmetry of a Drosophila central brain circuit originates from axonal interactions of two types of bilateral pioneer neurons. Subsequent recruitment of neighboring neurons into the asymmetric neuropil primordium results in hemisphere-specific microcircuits. Circuit lateralization requires dynamic expression of the cell adhesion molecule Fasciclin 2 to maintain structural plasticity in axonal remodeling. Reduced circuit asymmetry following cell type–specific Fasciclin 2 manipulation affects adult brain function. These results reveal an unexpected degree of developmental plasticity of late-born Drosophila neurons in the formation of a circuit node via the lateralized recruitment of symmetric circuit components."}],"OA_place":"publisher","fulldoi":"https://doi.org/10.1126/sciadv.aea6020","ddc":["570"],"article_number":"eaea6020","type":"journal_article","article_processing_charge":"Yes","doi":"10.1126/sciadv.aea6020","day":"27","quality_controlled":"1","oa":1,"date_updated":"2026-10-20T08:11:29Z","date_published":"2026-03-27T00:00:00Z","month":"03","citation":{"ama":"Markovitsch JW, Mitić D, Del Pilar Jiménez García A, et al. Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity. <i>Science Advances</i>. 2026;12(13). doi:<a href=\"https://doi.org/10.1126/sciadv.aea6020\">10.1126/sciadv.aea6020</a>","apa":"Markovitsch, J. W., Mitić, D., Del Pilar Jiménez García, A., Zane, A., Kainz, S., Kaur, R., &#38; Hummel, T. (2026). Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.aea6020\">https://doi.org/10.1126/sciadv.aea6020</a>","mla":"Markovitsch, Johann W., et al. “Sequential Formation of Drosophila Circuit Asymmetry via Prolonged Structural Plasticity.” <i>Science Advances</i>, vol. 12, no. 13, eaea6020, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aea6020\">10.1126/sciadv.aea6020</a>.","chicago":"Markovitsch, Johann W., Daniel Mitić, Alisa Del Pilar Jiménez García, Alsberga Zane, Sarah Kainz, Rashmit Kaur, and Thomas Hummel. “Sequential Formation of Drosophila Circuit Asymmetry via Prolonged Structural Plasticity.” <i>Science Advances</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/sciadv.aea6020\">https://doi.org/10.1126/sciadv.aea6020</a>.","ista":"Markovitsch JW, Mitić D, Del Pilar Jiménez García A, Zane A, Kainz S, Kaur R, Hummel T. 2026. Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity. Science Advances. 12(13), eaea6020.","ieee":"J. W. Markovitsch <i>et al.</i>, “Sequential formation of Drosophila circuit asymmetry via prolonged structural plasticity,” <i>Science Advances</i>, vol. 12, no. 13. AAAS, 2026.","short":"J.W. Markovitsch, D. Mitić, A. Del Pilar Jiménez García, A. Zane, S. Kainz, R. Kaur, T. Hummel, Science Advances 12 (2026)."},"department":[{"_id":"MiSi"},{"_id":"GradSch"}],"date_created":"2026-04-12T22:01:48Z","oa_version":"Published Version","issue":"13","volume":12,"OA_type":"gold","DOAJ_listed":"1","year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Science Advances","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"file":[{"content_type":"application/pdf","file_name":"2026_ScienceAdv_Markovitsch.pdf","success":1,"checksum":"3eed470fe73e53d2a8d55d6fba6934e3","access_level":"open_access","date_created":"2026-05-04T09:16:36Z","creator":"dernst","file_id":"21786","date_updated":"2026-05-04T09:16:36Z","file_size":11101140,"relation":"main_file"}],"language":[{"iso":"eng"}],"intvolume":"        12","publication_identifier":{"eissn":["2375-2548"]},"file_date_updated":"2026-05-04T09:16:36Z","status":"public"},{"publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-090-9 "]},"status":"public","file_date_updated":"2026-08-27T12:48:42Z","file":[{"file_size":27430796,"date_updated":"2026-08-27T12:48:42Z","creator":"mjavoor","file_id":"22767","relation":"source_file","file_name":"2026_Javoor_Manjunath_Thesis.docx","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2026-08-26T12:02:47Z","access_level":"closed","checksum":"f9c2847df9f1ac5a3d60c06b3b81a450"},{"relation":"main_file","file_id":"22768","date_updated":"2026-08-26T12:02:46Z","creator":"mjavoor","file_size":19489230,"embargo_to":"open_access","checksum":"8e9b4c0fcafbccf5c3796eacc9134a08","date_created":"2026-08-26T12:02:46Z","access_level":"closed","file_name":"2026_Javoor_Manjunath_Thesis.pdf","content_type":"application/pdf","embargo":"2027-08-21"}],"corr_author":"1","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"doi_confirm":"1","keyword":["Actin cytoskeleton","Cell migration","cryo-electron tomography"],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","related_material":{"record":[{"status":"public","id":"12334","relation":"part_of_dissertation"},{"status":"public","id":"21762","relation":"part_of_dissertation"},{"id":"12421","relation":"part_of_dissertation","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"19795"}]},"year":"2026","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"oa_version":"None","department":[{"_id":"GradSch"},{"_id":"FlSc"},{"_id":"MiSi"}],"alternative_title":["ISTA Thesis"],"date_created":"2026-08-21T09:11:04Z","citation":{"ista":"Javoor M. 2026. Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography. Institute of Science and Technology Austria .","short":"M. Javoor, Large-Scale Imaging of Cellular Actin Networks at Single Filament Resolution Using Montage Cryo-Electron Tomography, Institute of Science and Technology Austria , 2026.","ieee":"M. Javoor, “Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography,” Institute of Science and Technology Austria , 2026.","ama":"Javoor M. Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography. 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-22744\">10.15479/AT-ISTA-22744</a>","chicago":"Javoor, Manjunath. “Large-Scale Imaging of Cellular Actin Networks at Single Filament Resolution Using Montage Cryo-Electron Tomography.” Institute of Science and Technology Austria , 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-22744\">https://doi.org/10.15479/AT-ISTA-22744</a>.","apa":"Javoor, M. (2026). <i>Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography</i>. Institute of Science and Technology Austria . <a href=\"https://doi.org/10.15479/AT-ISTA-22744\">https://doi.org/10.15479/AT-ISTA-22744</a>","mla":"Javoor, Manjunath. <i>Large-Scale Imaging of Cellular Actin Networks at Single Filament Resolution Using Montage Cryo-Electron Tomography</i>. Institute of Science and Technology Austria , 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-22744\">10.15479/AT-ISTA-22744</a>."},"date_published":"2026-08-21T00:00:00Z","month":"08","date_updated":"2026-10-20T08:55:33Z","day":"21","project":[{"name":"A molecular atlas of Actin filament IDentities in the cell motility machinery","_id":"bd980d18-d553-11ed-ba76-ceaa645c97eb","grant_number":"101076260"},{"grant_number":"101071793","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces"}],"doi":"10.15479/AT-ISTA-22744","article_processing_charge":"No","supervisor":[{"last_name":"Schur","first_name":"Florian KM","orcid":"0000-0003-4790-8078","full_name":"Schur, Florian KM","id":"48AD8942-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"ddc":["570"],"type":"dissertation","fulldoi":"https://doi.org/10.15479/AT-ISTA-22744","OA_place":"publisher","OA_embargo":"12","degree_awarded":"PhD","has_accepted_license":"1","publisher":"Institute of Science and Technology Austria ","title":"Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography","author":[{"full_name":"Javoor, Manjunath","id":"305ab18b-dc7d-11ea-9b2f-b58195228ea2","first_name":"Manjunath","last_name":"Javoor","orcid":"0000-0003-2311-2112"}],"acknowledgement":"This work was supported by the ERC StG grant ActinID (PRA01221F1049A) awarded to Florian\r\nSchur, the ERC-SyG grant Pushing from within (P01071793) awarded to Michael Sixt, and by ISTA.\r\nI would like to thank the Scientific Service Units at ISTA for their essential support throughout\r\nthis work. In particular, I am grateful to the Electron Microscopy Facility, Imaging and Optics\r\nFacility, Zebrafish Facility, Scientific Computing Facility, and Lab Support Facility for their services,\r\nand technical support, all of which were important for the successful completion of this project.","_id":"22744","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","publication_status":"published","page":"121"},{"publication_status":"published","page":"3845-3846","_id":"22144","scopus_import":"1","author":[{"full_name":"Riedl, Michael","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","first_name":"Michael","last_name":"Riedl","orcid":"0000-0003-4844-6311"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt"}],"title":"A new sense for electrical fields","publisher":"Cell Press","abstract":[{"text":"Most cells polarize and migrate in response to electrical fields. In this issue of Cell, Belliveau et al. identify TMEM154/Galvanin, a receptor that serves as a cellular antenna to sense electrical gradients and guide migration toward the cathode.","lang":"eng"}],"article_type":"comment","type":"journal_article","fulldoi":"https://doi.org/10.1016/j.cell.2026.05.038","article_processing_charge":"No","day":"25","doi":"10.1016/j.cell.2026.05.038","month":"06","date_published":"2026-06-25T00:00:00Z","date_updated":"2026-10-20T09:00:16Z","quality_controlled":"1","date_created":"2026-06-28T22:01:34Z","department":[{"_id":"MiSi"}],"citation":{"ama":"Riedl M, Sixt MK. A new sense for electrical fields. <i>Cell</i>. 2026;189(13):3845-3846. doi:<a href=\"https://doi.org/10.1016/j.cell.2026.05.038\">10.1016/j.cell.2026.05.038</a>","chicago":"Riedl, Michael, and Michael K Sixt. “A New Sense for Electrical Fields.” <i>Cell</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.cell.2026.05.038\">https://doi.org/10.1016/j.cell.2026.05.038</a>.","mla":"Riedl, Michael, and Michael K. Sixt. “A New Sense for Electrical Fields.” <i>Cell</i>, vol. 189, no. 13, Cell Press, 2026, pp. 3845–46, doi:<a href=\"https://doi.org/10.1016/j.cell.2026.05.038\">10.1016/j.cell.2026.05.038</a>.","apa":"Riedl, M., &#38; Sixt, M. K. (2026). A new sense for electrical fields. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2026.05.038\">https://doi.org/10.1016/j.cell.2026.05.038</a>","ista":"Riedl M, Sixt MK. 2026. A new sense for electrical fields. Cell. 189(13), 3845–3846.","short":"M. Riedl, M.K. Sixt, Cell 189 (2026) 3845–3846.","ieee":"M. Riedl and M. K. Sixt, “A new sense for electrical fields,” <i>Cell</i>, vol. 189, no. 13. Cell Press, pp. 3845–3846, 2026."},"das_tickbox":"0","volume":189,"issue":"13","OA_type":"closed access","researchdata_availability":"no","oa_version":"None","year":"2026","supplementarymaterial":"no","publication":"Cell","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","publication_identifier":{"eissn":["1097-4172"],"issn":["0092-8674"]},"intvolume":"       189","language":[{"iso":"eng"}],"corr_author":"1"},{"quality_controlled":"1","oa":1,"date_updated":"2025-12-30T09:00:42Z","date_published":"2025-09-01T00:00:00Z","month":"09","doi":"10.1084/jem.20240109","day":"01","oa_version":"Published Version","OA_type":"hybrid","issue":"9","volume":222,"citation":{"chicago":"Gawish, Riem, Rajagopal Varada, Florian Deckert, Anastasiya Hladik, Linda Steinbichl, Laura Cimatti, Katarina Milanovic, et al. “Filamin A Editing in Myeloid Cells Reduces Intestinal Inflammation and Protects from Colitis.” <i>Journal of Experimental Medicine</i>. Rockefeller University Press, 2025. <a href=\"https://doi.org/10.1084/jem.20240109\">https://doi.org/10.1084/jem.20240109</a>.","apa":"Gawish, R., Varada, R., Deckert, F., Hladik, A., Steinbichl, L., Cimatti, L., … Vesely, C. (2025). Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. <i>Journal of Experimental Medicine</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1084/jem.20240109\">https://doi.org/10.1084/jem.20240109</a>","mla":"Gawish, Riem, et al. “Filamin A Editing in Myeloid Cells Reduces Intestinal Inflammation and Protects from Colitis.” <i>Journal of Experimental Medicine</i>, vol. 222, no. 9, e20240109, Rockefeller University Press, 2025, doi:<a href=\"https://doi.org/10.1084/jem.20240109\">10.1084/jem.20240109</a>.","ama":"Gawish R, Varada R, Deckert F, et al. Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. <i>Journal of Experimental Medicine</i>. 2025;222(9). doi:<a href=\"https://doi.org/10.1084/jem.20240109\">10.1084/jem.20240109</a>","short":"R. Gawish, R. Varada, F. Deckert, A. Hladik, L. Steinbichl, L. Cimatti, K. Milanovic, M. Jain, N. Torgasheva, A. Tanzer, K. De Paepe, T. Van De Wiele, B. Hausmann, M. Lang, M. Pechhacker, N. Ibrahim, I. de Vries, C. Brostjan, M.K. Sixt, C. Gasche, L. Boon, D. Berry, M.F. Jantsch, F.C. Pereira, C. Vesely, Journal of Experimental Medicine 222 (2025).","ieee":"R. Gawish <i>et al.</i>, “Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis,” <i>Journal of Experimental Medicine</i>, vol. 222, no. 9. Rockefeller University Press, 2025.","ista":"Gawish R, Varada R, Deckert F, Hladik A, Steinbichl L, Cimatti L, Milanovic K, Jain M, Torgasheva N, Tanzer A, De Paepe K, Van De Wiele T, Hausmann B, Lang M, Pechhacker M, Ibrahim N, de Vries I, Brostjan C, Sixt MK, Gasche C, Boon L, Berry D, Jantsch MF, Pereira FC, Vesely C. 2025. Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. Journal of Experimental Medicine. 222(9), e20240109."},"department":[{"_id":"MiSi"}],"date_created":"2025-06-29T22:01:15Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Journal of Experimental Medicine","pmid":1,"external_id":{"isi":["001502896900001"],"pmid":["40471139"]},"year":"2025","file":[{"access_level":"open_access","date_created":"2025-12-30T09:00:04Z","checksum":"708d61fb8cf1d83ee1e33ddcfde0857e","success":1,"content_type":"application/pdf","file_name":"2025_JEM_Gawish.pdf","relation":"main_file","file_size":9349311,"date_updated":"2025-12-30T09:00:04Z","creator":"dernst","file_id":"20899"}],"language":[{"iso":"eng"}],"intvolume":"       222","publication_identifier":{"eissn":["1540-9538"],"issn":["0022-1007"]},"file_date_updated":"2025-12-30T09:00:04Z","status":"public","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publication_status":"published","isi":1,"author":[{"last_name":"Gawish","first_name":"Riem","full_name":"Gawish, Riem"},{"full_name":"Varada, Rajagopal","last_name":"Varada","first_name":"Rajagopal"},{"first_name":"Florian","last_name":"Deckert","full_name":"Deckert, Florian"},{"full_name":"Hladik, Anastasiya","last_name":"Hladik","first_name":"Anastasiya"},{"full_name":"Steinbichl, Linda","first_name":"Linda","last_name":"Steinbichl"},{"full_name":"Cimatti, Laura","first_name":"Laura","last_name":"Cimatti"},{"last_name":"Milanovic","first_name":"Katarina","full_name":"Milanovic, Katarina"},{"full_name":"Jain, Mamta","last_name":"Jain","first_name":"Mamta"},{"last_name":"Torgasheva","first_name":"Natalya","full_name":"Torgasheva, Natalya"},{"first_name":"Andrea","last_name":"Tanzer","full_name":"Tanzer, Andrea"},{"full_name":"De Paepe, Kim","first_name":"Kim","last_name":"De Paepe"},{"last_name":"Van De Wiele","first_name":"Tom","full_name":"Van De Wiele, Tom"},{"full_name":"Hausmann, Bela","last_name":"Hausmann","first_name":"Bela"},{"full_name":"Lang, Michaela","last_name":"Lang","first_name":"Michaela"},{"full_name":"Pechhacker, Martin","last_name":"Pechhacker","first_name":"Martin"},{"last_name":"Ibrahim","first_name":"Nahla","full_name":"Ibrahim, Nahla"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid","first_name":"Ingrid","last_name":"De Vries"},{"full_name":"Brostjan, Christine","last_name":"Brostjan","first_name":"Christine"},{"full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","orcid":"0000-0002-6620-9179"},{"last_name":"Gasche","first_name":"Christoph","full_name":"Gasche, Christoph"},{"last_name":"Boon","first_name":"Louis","full_name":"Boon, Louis"},{"last_name":"Berry","first_name":"David","full_name":"Berry, David"},{"full_name":"Jantsch, Michael F.","first_name":"Michael F.","last_name":"Jantsch"},{"first_name":"Fatima C.","last_name":"Pereira","full_name":"Pereira, Fatima C."},{"last_name":"Vesely","first_name":"Cornelia","full_name":"Vesely, Cornelia"}],"_id":"19928","scopus_import":"1","acknowledgement":"Sequencing was performed by the Vienna BioCenter Core Facilities (Medical University of Vienna Core Facility) and the Biomedical Sequencing Facility at CeMM, Vienna. Cell sorting and flow cytometry were performed at the Core Facility Flow Cytometry and Imaging (Medical University of Vienna). We thank Jasmin Schwarz, Gudrun Kohl, Petra Pjevac, and Joana Seneca Silva from the Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna for assisting with amplicon and metagenomic sequencing, as well as repositing of sequencing data. We thank Sophia Derdak and Michael Schuster for initial data analysis, Robert Vilvoi and Stephan Hemm for animal handling, Marcel Kertesz for mouse genotyping, and Salwan Roumaia for next generation sequencing sample preparation. Treatment schemes and graphical abstracts were created with https://BioRender.com.\r\n\r\nThis work was supported by the Austrian Science Fund, grant number ZK 57-B28 to C. Vesely, R. Gawish, and F.C. Pereira; grant number V 1025-B to R. Gawish; grant number DOC32-B28 to R. Varada and M.F. Jantsch; and F8007 and P32678 to M.F. Jantsch. Open Access funding provided by Medical University of Vienna.","OA_place":"publisher","fulldoi":"https://doi.org/10.1084/jem.20240109","ddc":["570"],"article_number":"e20240109","type":"journal_article","article_type":"original","has_accepted_license":"1","publisher":"Rockefeller University Press","abstract":[{"lang":"eng","text":"Patho-mechanistic origins of ulcerative colitis are still poorly understood. The actin cross-linker filamin A (FLNA) impacts cellular responses through interaction with cytosolic proteins. Posttranscriptional A-to-I editing generates two forms of FLNA: genome-encoded FLNAQ and FLNAR. FLNA is edited in colon fibroblasts, smooth muscle cells, and endothelial cells. We found that the FLNA editing status determines colitis severity. Editing was highest in healthy colons and reduced during murine and human colitis. Mice that exclusively express FLNAR were highly resistant to DSS-induced colitis, whereas fully FLNAQ animals developed severe inflammation. While the genetic induction of FLNA editing influenced transcriptional states of structural cells and microbiome composition, we found that FLNAR exerts protection specifically via myeloid cells, which are physiologically unedited. Introducing fixed FLNAR did not hamper cell migration but reduced macrophage inflammation and rendered neutrophils less prone to NETosis. Thus, loss of FLNA editing correlates with colitis severity, and targeted editing of myeloid cells serves as a novel therapeutic approach in intestinal inflammation."}],"title":"Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis","article_processing_charge":"Yes (via OA deal)"},{"OA_place":"publisher","fulldoi":"https://doi.org/10.1038/s41590-025-02211-w","ddc":["570"],"type":"journal_article","article_type":"letter_note","has_accepted_license":"1","title":"Migrating immune cells globally coordinate protrusive forces","abstract":[{"lang":"eng","text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence."}],"publisher":"Springer Nature","article_processing_charge":"Yes (via OA deal)","publication_status":"published","page":"1258–1266","isi":1,"author":[{"full_name":"Dos Reis Rodrigues, Patricia","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","last_name":"Dos Reis Rodrigues","first_name":"Patricia","orcid":"0000-0003-1681-508X"},{"full_name":"Avellaneda Sarrió, Mario","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","first_name":"Mario","last_name":"Avellaneda Sarrió","orcid":"0000-0001-6406-524X"},{"id":"3795523E-F248-11E8-B48F-1D18A9856A87","full_name":"Canigova, Nikola","orcid":"0000-0002-8518-5926","first_name":"Nikola","last_name":"Canigova"},{"full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","last_name":"Gärtner","orcid":"0000-0001-6120-3723"},{"full_name":"Vaahtomeri, Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87","last_name":"Vaahtomeri","first_name":"Kari","orcid":"0000-0001-7829-3518"},{"first_name":"Michael","last_name":"Riedl","orcid":"0000-0003-4844-6311","full_name":"Riedl, Michael","id":"3BE60946-F248-11E8-B48F-1D18A9856A87"},{"full_name":"De Vries, Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","last_name":"De Vries"},{"first_name":"Jack","last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild","orcid":"0000-0001-9843-3522"},{"full_name":"Fukui, Yoshinori","last_name":"Fukui","first_name":"Yoshinori"},{"orcid":"0000-0002-1009-9652","last_name":"Juanes Garcia","first_name":"Alba","id":"40F05888-F248-11E8-B48F-1D18A9856A87","full_name":"Juanes Garcia, Alba"},{"full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"scopus_import":"1","_id":"20082","acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","publication":"Nature Immunology","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","pmid":1,"external_id":{"pmid":["40664976"],"isi":["001529134300001"]},"related_material":{"record":[{"status":"public","id":"20149","relation":"dissertation_contains"}],"link":[{"url":"https://ista.ac.at/en/news/bench-pressing-cells/","description":"News on ISTA website","relation":"press_release"}]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"year":"2025","language":[{"iso":"eng"}],"file":[{"file_id":"20096","creator":"dernst","date_updated":"2025-07-31T08:00:33Z","file_size":13514646,"relation":"main_file","file_name":"2025_NatureImmunology_ReisRodrigues.pdf","content_type":"application/pdf","success":1,"checksum":"0c725123dca7797c682609bff2c4c5ac","date_created":"2025-07-31T08:00:33Z","access_level":"open_access"}],"PlanS_conform":"1","corr_author":"1","intvolume":"        26","publication_identifier":{"issn":["1529-2908"],"eissn":["1529-2916"]},"status":"public","file_date_updated":"2025-07-31T08:00:33Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"quality_controlled":"1","oa":1,"date_updated":"2026-04-28T13:26:50Z","date_published":"2025-08-01T00:00:00Z","month":"08","doi":"10.1038/s41590-025-02211-w","project":[{"grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"},{"grant_number":"944-2020","name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration","_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8"}],"day":"01","oa_version":"Published Version","OA_type":"hybrid","volume":26,"citation":{"ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","ieee":"P. Dos Reis Rodrigues <i>et al.</i>, “Migrating immune cells globally coordinate protrusive forces,” <i>Nature Immunology</i>, vol. 26. Springer Nature, pp. 1258–1266, 2025.","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.","ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. 2025;26:1258–1266. doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>","mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>.","apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>","chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>."},"department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"date_created":"2025-07-27T22:01:26Z"},{"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"NanoFab"}],"related_material":{"record":[{"status":"public","id":"10703","relation":"part_of_dissertation"},{"status":"public","id":"20082","relation":"part_of_dissertation"}]},"year":"2025","publication_identifier":{"issn":["2663-337X"]},"file_date_updated":"2025-08-27T13:02:28Z","status":"public","corr_author":"1","file":[{"content_type":"application/pdf","file_name":"2025_ReisRodrigues_Patricia_Thesis.pdf","success":1,"checksum":"fda8a1070667c3562263f4867609b41b","date_created":"2025-08-27T12:59:10Z","access_level":"open_access","file_id":"20232","date_updated":"2025-08-27T12:59:10Z","creator":"prodrigu","file_size":63885565,"relation":"main_file"},{"date_created":"2025-08-27T13:00:30Z","access_level":"closed","checksum":"e8b65affcbce846a926454df4b2867b9","file_name":"2025_ReisRodrigues_Patricia_Thesis.docx","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","relation":"source_file","file_size":50483434,"date_updated":"2025-08-27T13:02:28Z","creator":"prodrigu","file_id":"20233"}],"language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"date_published":"2025-08-08T00:00:00Z","month":"08","oa":1,"date_updated":"2026-04-28T13:26:50Z","day":"08","project":[{"grant_number":"101071793","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces"}],"doi":"10.15479/AT-ISTA-20149","oa_version":"Published Version","department":[{"_id":"GradSch"},{"_id":"MiSi"}],"alternative_title":["ISTA Thesis"],"date_created":"2025-08-08T09:18:02Z","citation":{"short":"P. Dos Reis Rodrigues, Coordination of Protrusive Forces in Immune Cell Migration , Institute of Science and Technology Austria, 2025.","ieee":"P. Dos Reis Rodrigues, “Coordination of protrusive forces in immune cell migration ,” Institute of Science and Technology Austria, 2025.","ista":"Dos Reis Rodrigues P. 2025. Coordination of protrusive forces in immune cell migration . Institute of Science and Technology Austria.","chicago":"Dos Reis Rodrigues, Patricia. “Coordination of Protrusive Forces in Immune Cell Migration .” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20149\">https://doi.org/10.15479/AT-ISTA-20149</a>.","apa":"Dos Reis Rodrigues, P. (2025). <i>Coordination of protrusive forces in immune cell migration </i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20149\">https://doi.org/10.15479/AT-ISTA-20149</a>","mla":"Dos Reis Rodrigues, Patricia. <i>Coordination of Protrusive Forces in Immune Cell Migration </i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20149\">10.15479/AT-ISTA-20149</a>.","ama":"Dos Reis Rodrigues P. Coordination of protrusive forces in immune cell migration . 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20149\">10.15479/AT-ISTA-20149</a>"},"ddc":["570"],"type":"dissertation","fulldoi":"https://doi.org/10.15479/AT-ISTA-20149","OA_place":"publisher","degree_awarded":"PhD","has_accepted_license":"1","publisher":"Institute of Science and Technology Austria","abstract":[{"text":"Immune responses depend on the coordinated and efficient migration of leukocytes. These\r\ncells, which are embedded and tightly confined within tissues, must navigate and traverse\r\ndiverse and complex three-dimensional environments. Leukocytes adapt their locomotory\r\nbehavior to the mechanical, geometrical, and biochemical characteristics of their\r\nsurroundings. In low-density environments, where the pore size of the interstitial matrix\r\nallows free passage, these cells position the nucleus directly behind the lamellipodium, the\r\nprotrusive actin structure that forms the leading front of the cell. In this configuration, they\r\nuse the nucleus as a gauge to identify the path of least resistance.\r\nHere, we show that in high-density environments, where the pore size precludes free passage\r\nof the cell body, leukocytes reposition the microtubule-organizing center (MTOC) and\r\nassociated organelles in front of the nucleus. In this configuration, they use actin structures\r\nprotruding orthogonally to the direction of migration in order to open a path for the cell body.\r\nWe identify two distinct actin populations that serve this purpose at different subcellular\r\nlocalizations. At the leading edge, local indentation of the plasma membrane leads to\r\nrecruitment of the Wiskott-Aldrich syndrome protein (WASp), which, via Arp2/3, results in\r\nthe formation of individual actin foci. At the cell body, actin polymerization is triggered by\r\nDOCK8, a Cdc42 exchange factor, resulting in the formation of a central actin pool.\r\nWe demonstrate that the central and peripheral actin pools are functionally communicating\r\nand that depletion of the central actin pool leads to increased actin accumulation at the cell\r\nfront, resulting in excessive extension of the leading edge.","lang":"eng"}],"title":"Coordination of protrusive forces in immune cell migration ","article_processing_charge":"No","supervisor":[{"orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"}],"publication_status":"published","page":"114","author":[{"full_name":"Dos Reis Rodrigues, Patricia","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","last_name":"Dos Reis Rodrigues","first_name":"Patricia","orcid":"0000-0003-1681-508X"}],"acknowledgement":"I would like to acknowledge the\r\nfinancial support of the European Research Council through the ERC-SyG grant “Pushing from\r\nwithin: Control of cell shape, integrity and motility by cytoskeletal pushing forces”\r\n(01071793), which made this research possible. ","_id":"20149"},{"publication_identifier":{"issn":["0027-8424"],"eissn":["1091-6490"]},"status":"public","file_date_updated":"2025-09-08T07:23:29Z","language":[{"iso":"eng"}],"file":[{"file_size":16069140,"date_updated":"2025-09-08T07:23:29Z","file_id":"20307","creator":"dernst","relation":"main_file","success":1,"file_name":"2025_PNAS_Ucar.pdf","content_type":"application/pdf","date_created":"2025-09-08T07:23:29Z","access_level":"open_access","checksum":"b36abd92673b6d76376fc9434bad52cc"}],"corr_author":"1","PlanS_conform":"1","intvolume":"       122","APC_amount":"5766,07 EUR","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"external_id":{"pmid":["40838890"],"isi":["001562181600001"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Proceedings of the National Academy of Sciences","pmid":1,"related_material":{"link":[{"url":"https://github.com/mehmetcanucar/Self-generated-chemotaxis","relation":"software"}]},"year":"2025","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"NanoFab"}],"oa_version":"Published Version","issue":"34","volume":122,"OA_type":"hybrid","department":[{"_id":"EdHa"},{"_id":"MiSi"}],"date_created":"2025-09-07T22:01:32Z","citation":{"ama":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(34). doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>","chicago":"Ucar, Mehmet C, Alsberga Zane, Jonna H Alanko, Michael K Sixt, and Edouard B Hannezo. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>.","apa":"Ucar, M. C., Zane, A., Alanko, J. H., Sixt, M. K., &#38; Hannezo, E. B. (2025). Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>","mla":"Ucar, Mehmet C., et al. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34, e2504064122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>.","ista":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. 2025. Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. 122(34), e2504064122.","short":"M.C. Ucar, A. Zane, J.H. Alanko, M.K. Sixt, E.B. Hannezo, Proceedings of the National Academy of Sciences 122 (2025).","ieee":"M. C. Ucar, A. Zane, J. H. Alanko, M. K. Sixt, and E. B. Hannezo, “Self-generated chemotaxis of mixed cell populations,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34. National Academy of Sciences, 2025."},"date_published":"2025-08-26T00:00:00Z","month":"08","quality_controlled":"1","date_updated":"2026-05-20T08:59:54Z","oa":1,"project":[{"name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","call_identifier":"H2020"}],"day":"26","doi":"10.1073/pnas.2504064122","article_processing_charge":"Yes (in subscription journal)","article_number":"e2504064122","ddc":["570"],"type":"journal_article","OA_place":"publisher","fulldoi":"https://doi.org/10.1073/pnas.2504064122","article_type":"original","has_accepted_license":"1","title":"Self-generated chemotaxis of mixed cell populations","abstract":[{"lang":"eng","text":"Cell and tissue movement in development, cancer invasion, and immune response relies on chemical or mechanical guidance cues. In many systems, this behavior is locally directed by self-generated signaling gradients rather than long-range, prepatterned cues. However, how heterogeneous mixtures of cells interact nonreciprocally and navigate through self-generated gradients remains largely unexplored. Here, we introduce a theoretical framework for the self-organized chemotaxis of heterogeneous cell populations. We find that the relative chemotactic sensitivities of different cell populations control their long-time coupling and comigration dynamics, with boundary conditions such as external cell and attractant reservoirs substantially influencing the migration patterns. Our model predicts an optimal parameter regime that enables robust and colocalized migration. We test our theoretical predictions with in vitro experiments demonstrating the comigration of distinct immune cell populations, and quantitatively reproduce observed migration patterns under wild-type and perturbed conditions. Interestingly, immune cell comigration occurs close to the predicted optimal regime. Finally, we incorporate mechanical interactions into our framework, revealing a nontrivial interplay between chemotactic and mechanical nonreciprocity in driving collective migration. Together, our findings suggest that self-generated chemotaxis is a robust strategy for the navigation of mixed cell populations."}],"publisher":"National Academy of Sciences","author":[{"first_name":"Mehmet C","last_name":"Ucar","orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425"},{"last_name":"Zane","first_name":"Alsberga","orcid":"0009-0003-0415-7603","full_name":"Zane, Alsberga","id":"60f7509a-f652-11ea-9d86-b963d6490d7c"},{"first_name":"Jonna H","last_name":"Alanko","orcid":"0000-0002-7698-3061","full_name":"Alanko, Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"},{"orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"}],"acknowledgement":"We thank all members of the M.S. and E.H. groups for stimulating discussions.We thank the Imaging and Optics facility, the Pre-clinical and Lab Support facility of the Institute of Science and Technology Austria for their excellent support and provided resources for the experimental research. In particular, we thank Jack Merrin from the Nanofabrication facility who generated the microfabricated channel used in this study. This work received funding fromt he European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 851288 to E.H.). M.C.U.is funded by a University of Sheffield Strategic Research Fellowship in the Physics of Life and Quantitative Biology.","scopus_import":"1","_id":"20289","publication_status":"published","ec_funded":1,"isi":1},{"article_processing_charge":"Yes","has_accepted_license":"1","article_type":"original","publisher":"Cell Press","abstract":[{"text":"Cell migration is a fundamental process during embryonic development. Most studies in vivo have focused on the migration of cells using the extracellular matrix (ECM) as their substrate for migration. In contrast, much less is known about how cells migrate on other cells, as found in early embryos when the ECM has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the early zebrafish gastrula use the ectoderm as their substrate for migration. We show that the lateral ectoderm is permissive for the animal-pole-directed migration of LME cells, while the ectoderm at the animal pole halts it. These differences in permissiveness depend on the lateral ectoderm being more cohesive than the animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion as one critical factor in regulating LME migration during zebrafish gastrulation.","lang":"eng"}],"title":"BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation","article_number":"115387","ddc":["570"],"type":"journal_article","OA_place":"publisher","fulldoi":"https://doi.org/10.1016/j.celrep.2025.115387","acknowledgement":"We are grateful to the colleagues who contributed to this work with discussions, technical advice, and feedback on the manuscript: Irene Steccari, David Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship (ALTF 1159-2018).","_id":"19404","scopus_import":"1","author":[{"full_name":"Tavano, Ste","id":"2F162F0C-F248-11E8-B48F-1D18A9856A87","last_name":"Tavano","first_name":"Ste","orcid":"0000-0001-9970-7804"},{"id":"e1e86031-6537-11eb-953a-f7ab92be508d","full_name":"Brückner, David","orcid":"0000-0001-7205-2975","first_name":"David","last_name":"Brückner"},{"last_name":"Tasciyan","first_name":"Saren","orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Tong","first_name":"Xin","id":"50F65CDC-AA30-11E9-A72B-8A12E6697425","full_name":"Tong, Xin"},{"last_name":"Kardos","first_name":"Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87","full_name":"Kardos, Roland"},{"id":"30A536BA-F248-11E8-B48F-1D18A9856A87","full_name":"Schauer, Alexandra","orcid":"0000-0001-7659-9142","last_name":"Schauer","first_name":"Alexandra"},{"full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","orcid":"0000-0001-9843-3522"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"isi":1,"publication_status":"published","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"status":"public","file_date_updated":"2025-03-17T10:26:54Z","file":[{"file_size":9067797,"file_id":"19413","creator":"dernst","date_updated":"2025-03-17T10:26:54Z","relation":"main_file","success":1,"file_name":"2025_CellReports_Tavano.pdf","content_type":"application/pdf","date_created":"2025-03-17T10:26:54Z","access_level":"open_access","checksum":"57e05dd1598c807af0afdb32cec039d3"}],"language":[{"iso":"eng"}],"corr_author":"1","intvolume":"        44","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"year":"2025","DOAJ_listed":"1","external_id":{"pmid":["40057955"],"isi":["001443652700001"]},"publication":"Cell Reports","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"Bio"}],"date_created":"2025-03-16T23:01:24Z","citation":{"ama":"Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>","chicago":"Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>.","apa":"Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A., … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>","mla":"Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>, vol. 44, no. 3, 115387, Cell Press, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>.","ista":"Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R, Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. Cell Reports. 44(3), 115387.","short":"S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild, C.-P.J. Heisenberg, Cell Reports 44 (2025).","ieee":"S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Cell Press, 2025."},"oa_version":"Published Version","OA_type":"gold","issue":"3","volume":44,"project":[{"_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","name":"A mechano-chemical theory for stem cell fate decisions in organoid development","grant_number":"ALTF 343-2022"},{"grant_number":"ALTF 1159-2018","name":"Mechanosensation in cell migration: the role of friction forces in cell polarization and directed migration","_id":"269CD5C4-B435-11E9-9278-68D0E5697425"}],"day":"25","doi":"10.1016/j.celrep.2025.115387","date_published":"2025-03-25T00:00:00Z","month":"03","quality_controlled":"1","oa":1,"date_updated":"2026-10-20T08:20:45Z"},{"issue":"18","volume":35,"OA_type":"closed access","oa_version":"None","date_created":"2025-10-05T22:01:35Z","department":[{"_id":"MiSi"}],"citation":{"ama":"LI Z, Sixt MK. Cell migration: How animal cells run and tumble. <i>Current Biology</i>. 2025;35(18):R890-R892. doi:<a href=\"https://doi.org/10.1016/j.cub.2025.08.016\">10.1016/j.cub.2025.08.016</a>","apa":"LI, Z., &#38; Sixt, M. K. (2025). Cell migration: How animal cells run and tumble. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2025.08.016\">https://doi.org/10.1016/j.cub.2025.08.016</a>","mla":"LI, ZIQIANG, and Michael K. Sixt. “Cell Migration: How Animal Cells Run and Tumble.” <i>Current Biology</i>, vol. 35, no. 18, Cell Press, 2025, pp. R890–92, doi:<a href=\"https://doi.org/10.1016/j.cub.2025.08.016\">10.1016/j.cub.2025.08.016</a>.","chicago":"LI, ZIQIANG, and Michael K Sixt. “Cell Migration: How Animal Cells Run and Tumble.” <i>Current Biology</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.cub.2025.08.016\">https://doi.org/10.1016/j.cub.2025.08.016</a>.","ista":"LI Z, Sixt MK. 2025. Cell migration: How animal cells run and tumble. Current Biology. 35(18), R890–R892.","ieee":"Z. LI and M. K. Sixt, “Cell migration: How animal cells run and tumble,” <i>Current Biology</i>, vol. 35, no. 18. Cell Press, pp. R890–R892, 2025.","short":"Z. LI, M.K. Sixt, Current Biology 35 (2025) R890–R892."},"month":"09","date_published":"2025-09-22T00:00:00Z","date_updated":"2026-10-20T08:55:09Z","quality_controlled":"1","day":"22","doi":"10.1016/j.cub.2025.08.016","status":"public","publication_identifier":{"eissn":["1879-0445"]},"intvolume":"        35","language":[{"iso":"eng"}],"corr_author":"1","external_id":{"isi":["001592664700001"],"pmid":["40987270"]},"pmid":1,"publication":"Current Biology","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","author":[{"full_name":"Li, Ziqiang","id":"922e68bb-1727-11ee-857c-966e8cc1b6c3","last_name":"Li","first_name":"Ziqiang"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"scopus_import":"1","_id":"20427","page":"R890-R892","publication_status":"published","isi":1,"article_processing_charge":"No","type":"journal_article","fulldoi":"https://doi.org/10.1016/j.cub.2025.08.016","publisher":"Cell Press","abstract":[{"lang":"eng","text":"Animal cells migrating up chemotactic gradients often show speed oscillations. A new study describes a molecular circuit that switches zebrafish germ cells between phases of straight runs, tumbling and directional reorientation."}],"title":"Cell migration: How animal cells run and tumble","article_type":"letter_note"},{"fulldoi":"https://doi.org/10.1111/jdv.20291","OA_place":"publisher","type":"journal_article","ddc":["570"],"publisher":"Wiley","title":"Langerhans cells: Central players in the pathophysiology of atopic dermatitis","abstract":[{"text":"Atopic dermatitis (AD) is the most common chronic inflammatory skin disease worldwide. AD is a highly complex disease with different subtypes. Many elements of AD pathophysiology have been described, but if/how they interact with each other or which mechanisms are important in which patients is still unclear. Langerhans cells (LCs) are antigen-presenting cells (APCs) in the epidermis. Depending on the context, they can act either pro- or anti-inflammatory. Many different studies have investigated LCs in the context of AD and found them to be connected to all major mechanisms of AD pathophysiology. As APCs, LCs recruit other immune cells and shape the immune response, especially adaptive immunity via polarization of T cells. As sentinel cells, LCs are primary sensors of the skin microbiome and are important for the decision of immunity versus tolerance. LCs are also involved with the integrity of the skin barrier by influencing tight junctions. Finally, LCs are important cells in the neuro-immune crosstalk in the skin. In this review, we provide an overview about the many different roles of LCs in AD. Understanding LCs might bring us closer to a more complete understanding of this highly complex disease. Potentially, modulating LCs might offer new options for targeted therapies for AD patients.","lang":"eng"}],"has_accepted_license":"1","article_type":"original","article_processing_charge":"Yes (in subscription journal)","publication_status":"published","page":"278-289","isi":1,"author":[{"full_name":"Pan, Yi","last_name":"Pan","first_name":"Yi"},{"full_name":"Hochgerner, Mathias","last_name":"Hochgerner","first_name":"Mathias"},{"last_name":"Cichon","first_name":"Malgorzata Anna","id":"d63197a3-c188-11ed-9387-8d33a3f13871","full_name":"Cichon, Malgorzata Anna"},{"first_name":"Theresa","last_name":"Benezeder","full_name":"Benezeder, Theresa"},{"full_name":"Bieber, Thomas","first_name":"Thomas","last_name":"Bieber"},{"full_name":"Wolf, Peter","last_name":"Wolf","first_name":"Peter"}],"_id":"17459","scopus_import":"1","acknowledgement":"This work was supported by the CK-CARE of the KühneFoundation, Switzerland; the China Scholarship Counciland Shanghai Biocelline Enterprise Co. Ltd, China.","pmid":1,"publication":"Journal of the European Academy of Dermatology and Venereology","supplementarymaterial":"no","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","external_id":{"pmid":["39157943"],"isi":["001292894900001"]},"dataavailabilitystatement":"Data sharing not applicable to this article as no data sets were generated or analysed during the review.","year":"2025","intvolume":"        39","file":[{"access_level":"open_access","date_created":"2025-04-16T09:59:37Z","checksum":"12555ddb3490daf10b8d44e334e7312e","success":1,"file_name":"2025_JEADV_Pan.pdf","content_type":"application/pdf","relation":"main_file","file_size":457698,"creator":"dernst","date_updated":"2025-04-16T09:59:37Z","file_id":"19583"}],"language":[{"iso":"eng"}],"status":"public","file_date_updated":"2025-04-16T09:59:37Z","publication_identifier":{"issn":["0926-9959"],"eissn":["1468-3083"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-10-21T07:59:34Z","oa":1,"quality_controlled":"1","month":"02","date_published":"2025-02-01T00:00:00Z","doi":"10.1111/jdv.20291","day":"01","volume":39,"OA_type":"hybrid","issue":"2","researchdata_availability":"not applicable","oa_version":"Published Version","citation":{"short":"Y. Pan, M. Hochgerner, M.A. Cichon, T. Benezeder, T. Bieber, P. Wolf, Journal of the European Academy of Dermatology and Venereology 39 (2025) 278–289.","ieee":"Y. Pan, M. Hochgerner, M. A. Cichon, T. Benezeder, T. Bieber, and P. Wolf, “Langerhans cells: Central players in the pathophysiology of atopic dermatitis,” <i>Journal of the European Academy of Dermatology and Venereology</i>, vol. 39, no. 2. Wiley, pp. 278–289, 2025.","ista":"Pan Y, Hochgerner M, Cichon MA, Benezeder T, Bieber T, Wolf P. 2025. Langerhans cells: Central players in the pathophysiology of atopic dermatitis. Journal of the European Academy of Dermatology and Venereology. 39(2), 278–289.","chicago":"Pan, Yi, Mathias Hochgerner, Malgorzata Anna Cichon, Theresa Benezeder, Thomas Bieber, and Peter Wolf. “Langerhans Cells: Central Players in the Pathophysiology of Atopic Dermatitis.” <i>Journal of the European Academy of Dermatology and Venereology</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/jdv.20291\">https://doi.org/10.1111/jdv.20291</a>.","mla":"Pan, Yi, et al. “Langerhans Cells: Central Players in the Pathophysiology of Atopic Dermatitis.” <i>Journal of the European Academy of Dermatology and Venereology</i>, vol. 39, no. 2, Wiley, 2025, pp. 278–89, doi:<a href=\"https://doi.org/10.1111/jdv.20291\">10.1111/jdv.20291</a>.","apa":"Pan, Y., Hochgerner, M., Cichon, M. A., Benezeder, T., Bieber, T., &#38; Wolf, P. (2025). Langerhans cells: Central players in the pathophysiology of atopic dermatitis. <i>Journal of the European Academy of Dermatology and Venereology</i>. Wiley. <a href=\"https://doi.org/10.1111/jdv.20291\">https://doi.org/10.1111/jdv.20291</a>","ama":"Pan Y, Hochgerner M, Cichon MA, Benezeder T, Bieber T, Wolf P. Langerhans cells: Central players in the pathophysiology of atopic dermatitis. <i>Journal of the European Academy of Dermatology and Venereology</i>. 2025;39(2):278-289. doi:<a href=\"https://doi.org/10.1111/jdv.20291\">10.1111/jdv.20291</a>"},"das_tickbox":"1","date_created":"2024-08-25T22:01:07Z","department":[{"_id":"MiSi"}]},{"publisher":"Institute of Science and Technology Austria","abstract":[{"text":"Cell migration is a crucial process in animal development and maintenance. It is incredibly\r\nheterogeneous, with different cell types utilizing fundamentally distinct migration strategies.\r\nThe strategies also depend on the cellular microenvironment, where cells can switch between\r\nmigration modes as they encounter new environmental cues. In this thesis, we investigated\r\nhow dendritic cells adapt their migration strategy when encountering geometrically,\r\nmechanically and chemically distinct environments.\r\nWhen dendritic cells are embedded in a homogeneous fibrous network, they migrate in a fast\r\nand directional amoeboid manner. In this migration strategy, extracellular proteolysis and\r\nintegrin-mediated adhesions are dispensable. Instead, the cells use topography of the\r\nenvironment to propel their cell body forward. To migrate efficiently in the maze of different\r\npore sizes, they position the nucleus ahead of the microtubule organizing center (MTOC) and\r\nuse it to gauge the pores to identify the path of least resistance. Our aim was to identify\r\nwhether dendritic cells adapt their migration strategy when encountering asymmetrical\r\ntransitions into much denser environments with limited choice of large pores. In such invasive\r\ntransitions it is unclear if the cells can cross tight pores without the use of adhesions and\r\nextracellular proteolysis and whether they maintain the nucleus in the cell front.\r\nUsing various cell migration assays such as fibrous 3D collagen gels, geometrically defined\r\nmicrochannels with constrictions and simplistic under agarose migration assay, we provide\r\na comprehensive characterization of invasive migration of dendritic cells. We show that\r\nduring invasion the cells stall and stretch, reflecting the difficulty to translocate the bulky cell\r\nbody into the dense environment. In collagen gels, we show that dendritic cells can invade\r\nwithout proteolysis and adhesions. Instead, they utilize contractility, which can lead to largescale collagen compressions. During invasion, the nucleus stalls at tight constrictions, leading\r\nto a transient organelle reorientation. To resolve the stalling, upregulated rear contractility is\r\nrequired. This contractile force is simultaneously necessary for reverting the nucleus back to\r\nthe cell front after invasion and maintaining this positioning during permissive migration.\r\nA functional role of the reorientation was uncovered in the first collaboration project.\r\nA prominent central actin pool was identified around the MTOC, especially pronounced in\r\ndense and compressive environments. The actin pool was shown to generate pushing forces\r\nto dilate the space for cell translocation. These forces are only necessary in non-permissive\r\nenvironments, where the nucleus reorients to the cell rear, allowing the actin pool to\r\ngenerate space. In permissive environments where space generation is dispensable, the\r\nMTOC is located behind the nucleus and the actin cloud has reduced intensity, allowing more\r\nactin to be incorporated into the lamellipodium, speeding up migration.\r\nIn the second collaboration project, we investigated the effects of distinct chemical\r\nenvironments on dendritic cell migration. The strikingly persistent migration of these cells\r\nwas explained by their ability to modulate and even self-generate chemokine gradients. This\r\nallows the cells to migrate faster and more persistent in uniform chemokine fields compared\r\nto imposed chemokine gradients. The chemokine receptor CCR7 was identified as a crucial\r\nplayer in this process, both sensing the signal and internalizing the chemokine to create a sink.","lang":"eng"}],"title":"Adaptive strategies of dendritic cell migration in response to environmental cues","has_accepted_license":"1","degree_awarded":"PhD","OA_embargo":"6","fulldoi":"https://doi.org/10.15479/AT-ISTA-19745","OA_place":"publisher","type":"dissertation","ddc":["570"],"supervisor":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt"}],"article_processing_charge":"No","ec_funded":1,"publication_status":"published","page":"133","_id":"19745","acknowledgement":"This project has received funding from the Austrian Science Fund (FWF) via the doctorate\r\ncollege DK NanoCell and from the European Union’s Horizon 2020 research and innovation\r\nprogramme under the Marie Skłodowska-Curie Grant Agreement No. 665385.\r\n","author":[{"id":"3795523E-F248-11E8-B48F-1D18A9856A87","full_name":"Canigova, Nikola","orcid":"0000-0002-8518-5926","last_name":"Canigova","first_name":"Nikola"}],"year":"2025","related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"14274"}]},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","file":[{"date_created":"2025-05-28T07:38:17Z","access_level":"closed","checksum":"1a2d1525d19347fbb879ef57c02951bf","file_name":"NikolaCanigova_Thesis_final.docx","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","relation":"source_file","file_size":103879193,"embargo_to":"open_access","creator":"cchlebak","date_updated":"2025-11-27T23:30:02Z","file_id":"19748"},{"embargo":"2025-11-27","content_type":"application/pdf","file_name":"NikolaCanigova_Thesis_final_PDFA2a_fixed.pdf","date_created":"2025-05-28T07:39:53Z","access_level":"open_access","checksum":"c1d8f9a40a8e19fcf895373f4b773a46","file_size":194530600,"creator":"cchlebak","file_id":"19749","date_updated":"2025-11-27T23:30:02Z","relation":"main_file"}],"corr_author":"1","language":[{"iso":"eng"}],"file_date_updated":"2025-11-27T23:30:02Z","status":"public","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-058-9"]},"doi":"10.15479/AT-ISTA-19745","day":"27","project":[{"name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","call_identifier":"H2020"},{"call_identifier":"FWF","grant_number":"W01250-B20","name":"Nano-Analytics of Cellular Systems","_id":"265E2996-B435-11E9-9278-68D0E5697425"}],"oa":1,"date_updated":"2026-06-18T17:34:48Z","month":"05","date_published":"2025-05-27T00:00:00Z","citation":{"mla":"Canigova, Nikola. <i>Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19745\">10.15479/AT-ISTA-19745</a>.","apa":"Canigova, N. (2025). <i>Adaptive strategies of dendritic cell migration in response to environmental cues</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19745\">https://doi.org/10.15479/AT-ISTA-19745</a>","chicago":"Canigova, Nikola. “Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19745\">https://doi.org/10.15479/AT-ISTA-19745</a>.","ama":"Canigova N. Adaptive strategies of dendritic cell migration in response to environmental cues. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19745\">10.15479/AT-ISTA-19745</a>","ieee":"N. Canigova, “Adaptive strategies of dendritic cell migration in response to environmental cues,” Institute of Science and Technology Austria, 2025.","short":"N. Canigova, Adaptive Strategies of Dendritic Cell Migration in Response to Environmental Cues, Institute of Science and Technology Austria, 2025.","ista":"Canigova N. 2025. Adaptive strategies of dendritic cell migration in response to environmental cues. Institute of Science and Technology Austria."},"date_created":"2025-05-26T08:49:00Z","department":[{"_id":"MiSi"},{"_id":"GradSch"}],"alternative_title":["ISTA Thesis"],"oa_version":"Published Version"},{"related_material":{"record":[{"status":"public","id":"21439","relation":"research_data"},{"status":"public","id":"22963","relation":"later_version"},{"status":"public","id":"21423","relation":"dissertation_contains"}]},"year":"2025","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"bioRxiv","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"language":[{"iso":"eng"}],"corr_author":"1","status":"public","doi":"10.1101/2025.05.20.655037","day":"25","project":[{"_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","grant_number":"101071793"},{"grant_number":"26360","name":"Motile active matter models of migrating cells and chiral filaments","_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d"}],"oa":1,"date_updated":"2026-11-13T23:31:12Z","month":"09","date_published":"2025-09-25T00:00:00Z","citation":{"mla":"Dunajova, Zuzana, et al. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K., &#38; Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>","chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>.","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>","ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening,” <i>bioRxiv</i>. .","short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B. Hannezo, BioRxiv (n.d.).","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. bioRxiv, <a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>."},"das_tickbox":"1","date_created":"2026-03-11T08:40:06Z","department":[{"_id":"GradSch"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"AnSa"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.05.20.655037"}],"oa_version":"Preprint","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening","abstract":[{"lang":"eng","text":"While tumor malignancy has been extensively studied under the prism of genetic and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal interactions with the microenvironment. This raises the hitherto untested possibility that heterogeneity of the untransformed tumor stroma can actively fuel malignant progression. As biological heterogeneity is inherently difficult to control, we adopted a reductionist approach and let tumor cells invade micro-engineered environments harboring obstacles with precision-controlled geometry. We find that not only the presence of obstacles, but more surprisingly their spatial disorder, causes a drastic shift from a collective to a single-cell mode of invasion – comparable in strength to cadherin loss. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell detachments result both from local geometrical constraints and a global integration of spatial disorder over time. We show that different types of microenvironments map onto different universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang (KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with quenched disorder (KPZq). Our findings highlight generic physical principles for how the mode of cancer cell invasion depends on environmental heterogeneity, with potential implications to understand tumor evolution in vivo."}],"has_accepted_license":"1","fulldoi":"https://doi.org/10.1101/2025.05.20.655037","OA_place":"repository","type":"preprint","ddc":["539","570"],"article_processing_charge":"No","publication_status":"draft","_id":"21427","acknowledgement":"European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian Academy of Sciences, 26360","author":[{"first_name":"Zuzana","last_name":"Dunajova","id":"4B39F286-F248-11E8-B48F-1D18A9856A87","full_name":"Dunajova, Zuzana"},{"id":"4323B49C-F248-11E8-B48F-1D18A9856A87","full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X","last_name":"Tasciyan","first_name":"Saren"},{"last_name":"Majek","first_name":"Juraj","full_name":"Majek, Juraj","id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","first_name":"Jack"},{"first_name":"Erik","last_name":"Sahai","full_name":"Sahai, Erik"},{"full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","orcid":"0000-0002-6620-9179"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"}]},{"fulldoi":"https://doi.org/10.1038/s41567-023-02302-1","type":"journal_article","ddc":["530"],"abstract":[{"text":"Contraction and flow of the actin cell cortex have emerged as a common principle by which cells reorganize their cytoplasm and take shape. However, how these cortical flows interact with adjacent cytoplasmic components, changing their form and localization, and how this affects cytoplasmic organization and cell shape remains unclear. Here we show that in ascidian oocytes, the cooperative activities of cortical actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive oocyte cytoplasmic reorganization and shape changes following fertilization. We show that vegetal-directed cortical actomyosin flows, established upon oocyte fertilization, lead to both the accumulation of cortical actin at the vegetal pole of the zygote and compression and local buckling of the adjacent elastic solid-like myoplasm layer due to friction forces generated at their interface. Once cortical flows have ceased, the multiple myoplasm buckles resolve into one larger buckle, which again drives the formation of the contraction pole—a protuberance of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings reveal a mechanism where cortical actomyosin network flows determine cytoplasmic reorganization and cell shape by deforming adjacent cytoplasmic components through friction forces.","lang":"eng"}],"title":"Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization","publisher":"Springer Nature","has_accepted_license":"1","article_type":"original","article_processing_charge":"Yes (in subscription journal)","publication_status":"published","page":"310-321","isi":1,"author":[{"first_name":"Silvia","last_name":"Caballero Mancebo","orcid":"0000-0002-5223-3346","full_name":"Caballero Mancebo, Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Shinde","first_name":"Rushikesh","full_name":"Shinde, Rushikesh"},{"id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","full_name":"Bolger-Munro, Madison","orcid":"0000-0002-8176-4824","first_name":"Madison","last_name":"Bolger-Munro"},{"id":"3F920B30-F248-11E8-B48F-1D18A9856A87","full_name":"Peruzzo, Matilda","orcid":"0000-0002-3415-4628","last_name":"Peruzzo","first_name":"Matilda"},{"full_name":"Szep, Gregory","id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","last_name":"Szep","first_name":"Gregory"},{"full_name":"Steccari, Irene","id":"2705C766-9FE2-11EA-B224-C6773DDC885E","last_name":"Steccari","first_name":"Irene"},{"id":"CD573DF4-9ED3-11E9-9D77-3223E6697425","full_name":"Labrousse Arias, David","last_name":"Labrousse Arias","first_name":"David"},{"full_name":"Zheden, Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","last_name":"Zheden","orcid":"0000-0002-9438-4783"},{"last_name":"Merrin","first_name":"Jack","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Callan-Jones, Andrew","last_name":"Callan-Jones","first_name":"Andrew"},{"first_name":"Raphaël","last_name":"Voituriez","full_name":"Voituriez, Raphaël"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"_id":"14846","scopus_import":"1","acknowledgement":"We would like to thank A. McDougall, E. Hannezo and the Heisenberg lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Electron Microscopy Facility, Imaging and Optics Facility and the Nanofabrication Facility. This work was supported by a Joint Project Grant from the FWF (I 3601-B27).","pmid":1,"supplementarymaterial":"yes","publication":"Nature Physics","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","external_id":{"isi":["001138880800005"],"pmid":["38370025"]},"dataavailabilitystatement":"Source data are provided with this paper. All other data supporting the findings of this study are available from the corresponding author on request","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"NanoFab"}],"year":"2024","related_material":{"link":[{"url":"https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/","description":"News on ISTA Website","relation":"press_release"}]},"intvolume":"        20","file":[{"file_size":9897883,"creator":"dernst","date_updated":"2024-07-16T12:12:43Z","file_id":"17267","relation":"main_file","success":1,"content_type":"application/pdf","file_name":"2024_NaturePhysics_CaballeroMancebo.pdf","date_created":"2024-07-16T12:12:43Z","access_level":"open_access","checksum":"7891ebe7c900ae47469ab127031dd1ec"}],"corr_author":"1","language":[{"iso":"eng"}],"status":"public","file_date_updated":"2024-07-16T12:12:43Z","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-10-01T08:33:51Z","oa":1,"quality_controlled":"1","month":"02","date_published":"2024-02-01T00:00:00Z","doi":"10.1038/s41567-023-02302-1","day":"01","project":[{"call_identifier":"FWF","grant_number":"I03601","_id":"2646861A-B435-11E9-9278-68D0E5697425","name":"Control of embryonic cleavage pattern"}],"volume":20,"oa_version":"Published Version","researchdata_availability":"yes","citation":{"ama":"Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. 2024;20:310-321. doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>","mla":"Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>, vol. 20, Springer Nature, 2024, pp. 310–21, doi:<a href=\"https://doi.org/10.1038/s41567-023-02302-1\">10.1038/s41567-023-02302-1</a>.","apa":"Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G., Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>","chicago":"Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41567-023-02302-1\">https://doi.org/10.1038/s41567-023-02302-1</a>.","ista":"Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.","ieee":"S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature Physics</i>, vol. 20. Springer Nature, pp. 310–321, 2024.","short":"S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I. Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez, C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321."},"das_tickbox":"1","date_created":"2024-01-21T23:00:57Z","department":[{"_id":"CaHe"},{"_id":"JoFi"},{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"}]},{"file":[{"checksum":"aa004afc72d2489f0fb0fcbc9919fbbd","access_level":"open_access","date_created":"2024-07-22T06:16:11Z","content_type":"application/pdf","file_name":"2024_Nature_Gaertner.pdf","success":1,"relation":"main_file","file_id":"17286","date_updated":"2024-07-22T06:16:11Z","creator":"dernst","file_size":15704819}],"corr_author":"1","language":[{"iso":"eng"}],"intvolume":"       631","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"file_date_updated":"2024-07-22T06:16:11Z","status":"public","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"supplementarymaterial":"yes","publication":"Nature","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","pmid":1,"external_id":{"isi":["001281636500020"],"pmid":["38987596"]},"related_material":{"link":[{"relation":"software","url":"https://github.com/heiniglab/gaertner_megakaryocytes"}]},"year":"2024","dataavailabilitystatement":"Imaging and flow cytometry raw data are available on request. scRNA-seq data are accessible at the GEO (GSE261996). Bulk RNA-seq data are accessible at the GEO (GSE185488). Source data are provided with this paper.","researchdata_availability":"upon request","oa_version":"Published Version","volume":631,"das_tickbox":"1","citation":{"ista":"Gärtner FR, Ishikawa-Ankerhold H, Stutte S, Fu W, Weitz J, Dueck A, Nelakuditi B, Fumagalli V, Van Den Heuvel D, Belz L, Sobirova G, Zhang Z, Titova A, Navarro AM, Pekayvaz K, Lorenz M, Von Baumgarten L, Kranich J, Straub T, Popper B, Zheden V, Kaufmann W, Guo C, Piontek G, Von Stillfried S, Boor P, Colonna M, Clauß S, Schulz C, Brocker T, Walzog B, Scheiermann C, Aird WC, Nerlov C, Stark K, Petzold T, Engelhardt S, Sixt MK, Hauschild R, Rudelius M, Oostendorp RAJ, Iannacone M, Heinig M, Massberg S. 2024. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. Nature. 631, 645–653.","ieee":"F. R. Gärtner <i>et al.</i>, “Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis,” <i>Nature</i>, vol. 631. Springer Nature, pp. 645–653, 2024.","short":"F.R. Gärtner, H. Ishikawa-Ankerhold, S. Stutte, W. Fu, J. Weitz, A. Dueck, B. Nelakuditi, V. Fumagalli, D. Van Den Heuvel, L. Belz, G. Sobirova, Z. Zhang, A. Titova, A.M. Navarro, K. Pekayvaz, M. Lorenz, L. Von Baumgarten, J. Kranich, T. Straub, B. Popper, V. Zheden, W. Kaufmann, C. Guo, G. Piontek, S. Von Stillfried, P. Boor, M. Colonna, S. Clauß, C. Schulz, T. Brocker, B. Walzog, C. Scheiermann, W.C. Aird, C. Nerlov, K. Stark, T. Petzold, S. Engelhardt, M.K. Sixt, R. Hauschild, M. Rudelius, R.A.J. Oostendorp, M. Iannacone, M. Heinig, S. Massberg, Nature 631 (2024) 645–653.","ama":"Gärtner FR, Ishikawa-Ankerhold H, Stutte S, et al. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. <i>Nature</i>. 2024;631:645-653. doi:<a href=\"https://doi.org/10.1038/s41586-024-07671-y\">10.1038/s41586-024-07671-y</a>","apa":"Gärtner, F. R., Ishikawa-Ankerhold, H., Stutte, S., Fu, W., Weitz, J., Dueck, A., … Massberg, S. (2024). Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-024-07671-y\">https://doi.org/10.1038/s41586-024-07671-y</a>","mla":"Gärtner, Florian R., et al. “Plasmacytoid Dendritic Cells Control Homeostasis of Megakaryopoiesis.” <i>Nature</i>, vol. 631, Springer Nature, 2024, pp. 645–53, doi:<a href=\"https://doi.org/10.1038/s41586-024-07671-y\">10.1038/s41586-024-07671-y</a>.","chicago":"Gärtner, Florian R, Hellen Ishikawa-Ankerhold, Susanne Stutte, Wenwen Fu, Jutta Weitz, Anne Dueck, Bhavishya Nelakuditi, et al. “Plasmacytoid Dendritic Cells Control Homeostasis of Megakaryopoiesis.” <i>Nature</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41586-024-07671-y\">https://doi.org/10.1038/s41586-024-07671-y</a>."},"department":[{"_id":"EM-Fac"},{"_id":"MiSi"},{"_id":"Bio"}],"date_created":"2024-07-21T22:01:02Z","quality_controlled":"1","oa":1,"date_updated":"2026-10-01T11:17:11Z","date_published":"2024-07-18T00:00:00Z","month":"07","doi":"10.1038/s41586-024-07671-y","project":[{"call_identifier":"H2020","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"}],"day":"18","article_processing_charge":"Yes (in subscription journal)","fulldoi":"https://doi.org/10.1038/s41586-024-07671-y","ddc":["570"],"type":"journal_article","has_accepted_license":"1","article_type":"original","title":"Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis","abstract":[{"text":"Platelet homeostasis is essential for vascular integrity and immune defence1,2. Although the process of platelet formation by fragmenting megakaryocytes (MKs; thrombopoiesis) has been extensively studied, the cellular and molecular mechanisms required to constantly replenish the pool of MKs by their progenitor cells (megakaryopoiesis) remains unclear3,4. Here we use intravital imaging to track the cellular dynamics of megakaryopoiesis over days. We identify plasmacytoid dendritic cells (pDCs) as homeostatic sensors that monitor the bone marrow for apoptotic MKs and deliver IFNα to the MK niche triggering local on-demand proliferation and maturation of MK progenitors. This pDC-dependent feedback loop is crucial for MK and platelet homeostasis at steady state and under stress. pDCs are best known for their ability to function as vigilant detectors of viral infection5. We show that virus-induced activation of pDCs interferes with their function as homeostatic sensors of megakaryopoiesis. Consequently, activation of pDCs by SARS-CoV-2 leads to excessive megakaryopoiesis. Together, we identify a pDC-dependent homeostatic circuit that involves innate immune sensing and demand-adapted release of inflammatory mediators to maintain homeostasis of the megakaryocytic lineage.","lang":"eng"}],"publisher":"Springer Nature","author":[{"orcid":"0000-0001-6120-3723","first_name":"Florian R","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R"},{"last_name":"Ishikawa-Ankerhold","first_name":"Hellen","full_name":"Ishikawa-Ankerhold, Hellen"},{"full_name":"Stutte, Susanne","first_name":"Susanne","last_name":"Stutte"},{"full_name":"Fu, Wenwen","first_name":"Wenwen","last_name":"Fu"},{"full_name":"Weitz, Jutta","first_name":"Jutta","last_name":"Weitz"},{"first_name":"Anne","last_name":"Dueck","full_name":"Dueck, Anne"},{"full_name":"Nelakuditi, Bhavishya","first_name":"Bhavishya","last_name":"Nelakuditi"},{"full_name":"Fumagalli, Valeria","last_name":"Fumagalli","first_name":"Valeria"},{"first_name":"Dominic","last_name":"Van Den Heuvel","full_name":"Van Den Heuvel, Dominic"},{"first_name":"Larissa","last_name":"Belz","full_name":"Belz, Larissa"},{"full_name":"Sobirova, Gulnoza","first_name":"Gulnoza","last_name":"Sobirova"},{"full_name":"Zhang, Zhe","first_name":"Zhe","last_name":"Zhang"},{"full_name":"Titova, Anna","last_name":"Titova","first_name":"Anna"},{"full_name":"Navarro, Alejandro Martinez","last_name":"Navarro","first_name":"Alejandro Martinez"},{"first_name":"Kami","last_name":"Pekayvaz","full_name":"Pekayvaz, Kami"},{"last_name":"Lorenz","first_name":"Michael","full_name":"Lorenz, Michael"},{"full_name":"Von Baumgarten, Louisa","last_name":"Von Baumgarten","first_name":"Louisa"},{"last_name":"Kranich","first_name":"Jan","full_name":"Kranich, Jan"},{"last_name":"Straub","first_name":"Tobias","full_name":"Straub, Tobias"},{"full_name":"Popper, Bastian","last_name":"Popper","first_name":"Bastian"},{"id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","full_name":"Zheden, Vanessa","orcid":"0000-0002-9438-4783","first_name":"Vanessa","last_name":"Zheden"},{"last_name":"Kaufmann","first_name":"Walter","orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Chenglong","last_name":"Guo","full_name":"Guo, Chenglong"},{"full_name":"Piontek, Guido","last_name":"Piontek","first_name":"Guido"},{"first_name":"Saskia","last_name":"Von Stillfried","full_name":"Von Stillfried, Saskia"},{"last_name":"Boor","first_name":"Peter","full_name":"Boor, Peter"},{"full_name":"Colonna, Marco","first_name":"Marco","last_name":"Colonna"},{"first_name":"Sebastian","last_name":"Clauß","full_name":"Clauß, Sebastian"},{"last_name":"Schulz","first_name":"Christian","full_name":"Schulz, Christian"},{"last_name":"Brocker","first_name":"Thomas","full_name":"Brocker, Thomas"},{"last_name":"Walzog","first_name":"Barbara","full_name":"Walzog, Barbara"},{"full_name":"Scheiermann, Christoph","first_name":"Christoph","last_name":"Scheiermann"},{"full_name":"Aird, William C.","first_name":"William C.","last_name":"Aird"},{"first_name":"Claus","last_name":"Nerlov","full_name":"Nerlov, Claus"},{"last_name":"Stark","first_name":"Konstantin","full_name":"Stark, Konstantin"},{"full_name":"Petzold, Tobias","last_name":"Petzold","first_name":"Tobias"},{"full_name":"Engelhardt, Stefan","first_name":"Stefan","last_name":"Engelhardt"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert"},{"last_name":"Rudelius","first_name":"Martina","full_name":"Rudelius, Martina"},{"full_name":"Oostendorp, Robert A.J.","first_name":"Robert A.J.","last_name":"Oostendorp"},{"last_name":"Iannacone","first_name":"Matteo","full_name":"Iannacone, Matteo"},{"last_name":"Heinig","first_name":"Matthias","full_name":"Heinig, Matthias"},{"last_name":"Massberg","first_name":"Steffen","full_name":"Massberg, Steffen"}],"scopus_import":"1","_id":"17284","acknowledgement":"We thank S. Helmer, N. Blount, E. Raatz and Z. Sisic for technical assistance. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) SFB 1123 (S.M. project B06); SFB 914 (S.M. projects B02 and Z01, H.I.-A. project Z01, S.S. project A06, K.S. project B02, C. Schulz project A10, B.W. project A02, C. Scheiermann project B09); SFB 1054 (T.B. project B03); FOR2033 (F.G., R.A.J.O., S.M.); Individual research grant project ID: 514478744 (F.G.); Heisenberg Programme project ID: 514477451 (F.G.); the DZHK (German Center for Cardiovascular Research) (MHA 1.4VD (S.M.), Postdoc Start-up Grant, 81×3600213 (F.G.)); and LMUexcellence NFF (F.G.). W.F. received funding from China Scholarship Council (CSC, no. 201306270012). P.B. is supported by the German Research Foundation (DFG, project IDs 322900939, 432698239 and 445703531), European Research Council (ERC Consolidator grant no. 101001791) and the Federal Ministry of Education and Research (BMBF, STOP-FSGS-01GM2202C and NATON within the framework of the Network of University Medicine, no. 01KX2121). S.v.S. is supported by the START-Program of the Faculty of Medicine of the RWTH Aachen University (AZ 125/17). A.D. and S.E. are supported by the German Research Foundation (SFB TRR 267); S.E. by the BMBF in the framework of the Cluster4future program (CNATM—Cluster for Nucleic Acid Therapeutics Munich). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 833440 to S.M.). F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687. The project is funded by the European Union (ERC, MEKanics, 101078110). Views and opinions expressed are those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council Executive Agency. Neither the European Union nor the granting authority can be held responsible for them.","publication_status":"published","page":"645-653","isi":1,"ec_funded":1},{"department":[{"_id":"FlSc"},{"_id":"MiSi"},{"_id":"Bio"},{"_id":"EM-Fac"}],"date_created":"2024-03-21T06:45:51Z","das_tickbox":"1","citation":{"ista":"Zens B, Fäßler F, Hansen J, Hauschild R, Datler J, Hodirnau V-V, Zheden V, Alanko JH, Sixt MK, Schur FK. 2024. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. Journal of Cell Biology. 223(6), e202309125.","short":"B. Zens, F. Fäßler, J. Hansen, R. Hauschild, J. Datler, V.-V. Hodirnau, V. Zheden, J.H. Alanko, M.K. Sixt, F.K. Schur, Journal of Cell Biology 223 (2024).","ieee":"B. Zens <i>et al.</i>, “Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix,” <i>Journal of Cell Biology</i>, vol. 223, no. 6. Rockefeller University Press, 2024.","ama":"Zens B, Fäßler F, Hansen J, et al. Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. <i>Journal of Cell Biology</i>. 2024;223(6). doi:<a href=\"https://doi.org/10.1083/jcb.202309125\">10.1083/jcb.202309125</a>","chicago":"Zens, Bettina, Florian Fäßler, Jesse Hansen, Robert Hauschild, Julia Datler, Victor-Valentin Hodirnau, Vanessa Zheden, Jonna H Alanko, Michael K Sixt, and Florian KM Schur. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2024. <a href=\"https://doi.org/10.1083/jcb.202309125\">https://doi.org/10.1083/jcb.202309125</a>.","mla":"Zens, Bettina, et al. “Lift-out Cryo-FIBSEM and Cryo-ET Reveal the Ultrastructural Landscape of Extracellular Matrix.” <i>Journal of Cell Biology</i>, vol. 223, no. 6, e202309125, Rockefeller University Press, 2024, doi:<a href=\"https://doi.org/10.1083/jcb.202309125\">10.1083/jcb.202309125</a>.","apa":"Zens, B., Fäßler, F., Hansen, J., Hauschild, R., Datler, J., Hodirnau, V.-V., … Schur, F. K. (2024). Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202309125\">https://doi.org/10.1083/jcb.202309125</a>"},"oa_version":"Published Version","researchdata_availability":"yes","issue":"6","volume":223,"day":"20","project":[{"_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","name":"Structure and isoform diversity of the Arp2/3 complex","grant_number":"P33367"},{"_id":"7bd318a1-9f16-11ee-852c-cc9217763180","name":"In Situ Actin Structures via Hybrid Cryo-electron Microscopy","grant_number":"E435"},{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients","grant_number":"724373","call_identifier":"H2020"},{"_id":"059B463C-7A3F-11EA-A408-12923DDC885E","name":"NÖ-Fonds Preis für die Jungforscherin des Jahres am IST Austria"},{"_id":"2615199A-B435-11E9-9278-68D0E5697425","name":"Spatiotemporal regulation of chemokine-induced signalling in leukocyte chemotaxis","grant_number":"21317"},{"name":"CryoMinflux-guided in-situ visual proteomics and structure determination","_id":"62909c6f-2b32-11ec-9570-e1476aab5308","grant_number":"CZI01"}],"doi":"10.1083/jcb.202309125","date_published":"2024-03-20T00:00:00Z","month":"03","quality_controlled":"1","oa":1,"date_updated":"2026-10-01T11:24:27Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publication_identifier":{"eissn":["1540-8140"],"issn":["0021-9525"]},"status":"public","file_date_updated":"2024-03-25T12:52:04Z","language":[{"iso":"eng"}],"corr_author":"1","file":[{"file_size":11907016,"creator":"dernst","file_id":"15188","date_updated":"2024-03-25T12:52:04Z","relation":"main_file","success":1,"content_type":"application/pdf","file_name":"2024_JCB_Zens.pdf","access_level":"open_access","date_created":"2024-03-25T12:52:04Z","checksum":"90d1984a93660735e506c2a304bc3f73"}],"intvolume":"       223","year":"2024","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"M-Shop"}],"dataavailabilitystatement":"Representative tomograms containing the data shown in Fig. 3, and Figs. S5 and S9 have been deposited in the Electron Microscopy Data Bank under accession codes: EMD-18490, EMD-18491, EMD-18492, EMD-18493, and EMD-18494. All tilt series, including frames, raw tilt series, and reconstructed IsoNet-filtered tomograms, were deposited in the Electron Microscopy Public Image Archive database under the accession code EMPIAR-11897.","external_id":{"pmid":["38506714"],"isi":["001264190100001"]},"publication":"Journal of Cell Biology","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","supplementarymaterial":"yes","pmid":1,"acknowledgement":"Open Access funding provided by IST Austria. We thank Armel Nicolas and his team at the ISTA proteomics facility, Alois Schloegl, Stefano Elefante, and colleagues at the ISTA Scientific Computing facility, Tommaso Constanzo and Ludek Lovicar at the Electron Microsocpy Facility (EMF), and Thomas Menner at the Miba Machine shop for their support. We also thank Wanda Kukulski (University of Bern) as well as Darío Porley, Andreas Thader, and other members of the Schur group for helpful discussions. Matt Swulius and Jessica Heebner provided great support in using Dragonfly. We thank Dorotea Fracciolla (Art & Science) for support in figure illustration.\r\n\r\nThis research was supported by the Scientific Service Units of ISTA through resources provided by Scientific Computing, the Lab Support Facility, and the Electron Microscopy Facility. We acknowledge funding support from the following sources: Austrian Science Fund (FWF) grant P33367 (to F.K.M. Schur), the Federation of European Biochemical Societies (to F.K.M. Schur), Niederösterreich (NÖ) Fonds (to B. Zens), FWF grant E435 (to J.M. Hansen), European Research Council under the European Union’s Horizon 2020 research (grant agreement No. 724373) (to M. Sixt), and Jenny and Antti Wihuri Foundation (to J. Alanko). This publication has been made possible in part by CZI grant DAF2021-234754 and grant DOI https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (to F.K.M. Schur).","scopus_import":"1","_id":"15146","author":[{"orcid":"0000-0002-9561-1239","first_name":"Bettina","last_name":"Zens","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","full_name":"Zens, Bettina"},{"full_name":"Fäßler, Florian","id":"404F5528-F248-11E8-B48F-1D18A9856A87","first_name":"Florian","last_name":"Fäßler","orcid":"0000-0001-7149-769X"},{"full_name":"Hansen, Jesse","id":"1063c618-6f9b-11ec-9123-f912fccded63","first_name":"Jesse","last_name":"Hansen","orcid":"0000-0001-7967-2085"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert"},{"id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87","full_name":"Datler, Julia","orcid":"0000-0002-3616-8580","first_name":"Julia","last_name":"Datler"},{"full_name":"Hodirnau, Victor-Valentin","id":"3661B498-F248-11E8-B48F-1D18A9856A87","first_name":"Victor-Valentin","last_name":"Hodirnau","orcid":"0000-0003-3904-947X"},{"full_name":"Zheden, Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","last_name":"Zheden","orcid":"0000-0002-9438-4783"},{"id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","full_name":"Alanko, Jonna H","orcid":"0000-0002-7698-3061","last_name":"Alanko","first_name":"Jonna H"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","full_name":"Schur, Florian KM","orcid":"0000-0003-4790-8078","first_name":"Florian KM","last_name":"Schur"}],"ec_funded":1,"isi":1,"publication_status":"published","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","article_type":"original","abstract":[{"lang":"eng","text":"The extracellular matrix (ECM) serves as a scaffold for cells and plays an essential role in regulating numerous cellular processes, including cell migration and proliferation. Due to limitations in specimen preparation for conventional room-temperature electron microscopy, we lack structural knowledge on how ECM components are secreted, remodeled, and interact with surrounding cells. We have developed a 3D-ECM platform compatible with sample thinning by cryo-focused ion beam milling, the lift-out extraction procedure, and cryo-electron tomography. Our workflow implements cell-derived matrices (CDMs) grown on EM grids, resulting in a versatile tool closely mimicking ECM environments. This allows us to visualize ECM for the first time in its hydrated, native context. Our data reveal an intricate network of extracellular fibers, their positioning relative to matrix-secreting cells, and previously unresolved structural entities. Our workflow and results add to the structural atlas of the ECM, providing novel insights into its secretion and assembly."}],"publisher":"Rockefeller University Press","title":"Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix","article_number":"e202309125","ddc":["570"],"type":"journal_article","fulldoi":"https://doi.org/10.1083/jcb.202309125"}]