[{"_id":"17233","publication_identifier":{"eissn":["2331-8325"]},"department":[{"_id":"MiSi"}],"oa":1,"date_published":"2024-07-05T00:00:00Z","article_type":"original","researchdata_availability":"no","scopus_import":"1","title":"Versatile cloning strategy for efficient multigene editing in Arabidopsis","article_number":"e5029","intvolume":"        14","volume":14,"abstract":[{"text":"CRISPR-Cas9 technology has become an essential tool for plant genome editing. Recent advancements have significantly improved the ability to target multiple genes simultaneously within the same genetic background through various strategies. Additionally, there has been significant progress in developing methods for inducible or tissue-specific editing. These advancements offer numerous possibilities for tailored genome modifications. Building upon existing research, we have developed an optimized and modular strategy allowing the targeting of several genes simultaneously in combination with the synchronized expression of the Cas9 endonuclease in the egg cell. This system allows significant editing efficiency while avoiding mosaicism. In addition, the versatile system we propose allows adaptation to inducible and/or tissue-specific edition according to the promoter chosen to drive the expression of the Cas9 gene. Here, we describe a step-by-step protocol for generating the binary vector necessary for establishing Arabidopsis edited lines using a versatile cloning strategy that combines Gateway® and Golden Gate technologies. We describe a versatile system that allows the cloning of as many guides as needed to target DNA, which can be multiplexed into a polycistronic gene and combined in the same construct with sequences for the expression of the Cas9 endonuclease. The expression of Cas9 is controlled by selecting from among a collection of promoters, including constitutive, inducible, ubiquitous, or tissue-specific promoters. Only one vector containing the polycistronic gene (tRNA-sgRNA) needs to be constructed. For that, sgRNA (composed of protospacers chosen to target the gene of interest and sgRNA scaffold) is cloned in tandem with the pre-tRNA sequence. Then, a single recombination reaction is required to assemble the promoter, the zCas9 coding sequence, and the tRNA-gRNA polycistronic gene. Each element is cloned in an entry vector and finally assembled according to the Multisite Gateway® Technology. Here, we detail the process to express zCas9 under the control of egg cell promoter fused to enhancer sequence (EC1.2en-EC1.1p) and to simultaneously target two multiple C2 domains and transmembrane region protein genes (MCTP3 and MCTP4, respectively at3g57880 and at1g51570), using one or two sgRNA per gene.","lang":"eng"}],"fulldoi":"https://doi.org/10.21769/BioProtoc.5029","day":"05","acknowledgement":"This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (project 772103-BRIDGING to E.M.B.).","year":"2024","das_tickbox":"0","doi":"10.21769/BioProtoc.5029","supplementarymaterial":"no","publication":"Bio-protocol","external_id":{"pmid":["39007160"]},"publisher":"Bio-Protocol","pmid":1,"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"chicago":"LI, ZIQIANG, Jennifer Huard, Emmanuelle M. Bayer, and Valérie Wattelet-Boyer. “Versatile Cloning Strategy for Efficient Multigene Editing in Arabidopsis.” <i>Bio-Protocol</i>. Bio-Protocol, 2024. <a href=\"https://doi.org/10.21769/BioProtoc.5029\">https://doi.org/10.21769/BioProtoc.5029</a>.","ama":"LI Z, Huard J, Bayer EM, Wattelet-Boyer V. Versatile cloning strategy for efficient multigene editing in Arabidopsis. <i>Bio-protocol</i>. 2024;14(13). doi:<a href=\"https://doi.org/10.21769/BioProtoc.5029\">10.21769/BioProtoc.5029</a>","ieee":"Z. LI, J. Huard, E. M. Bayer, and V. Wattelet-Boyer, “Versatile cloning strategy for efficient multigene editing in Arabidopsis,” <i>Bio-protocol</i>, vol. 14, no. 13. Bio-Protocol, 2024.","apa":"LI, Z., Huard, J., Bayer, E. M., &#38; Wattelet-Boyer, V. (2024). Versatile cloning strategy for efficient multigene editing in Arabidopsis. <i>Bio-Protocol</i>. Bio-Protocol. <a href=\"https://doi.org/10.21769/BioProtoc.5029\">https://doi.org/10.21769/BioProtoc.5029</a>","ista":"LI Z, Huard J, Bayer EM, Wattelet-Boyer V. 2024. Versatile cloning strategy for efficient multigene editing in Arabidopsis. Bio-protocol. 14(13), e5029.","short":"Z. LI, J. Huard, E.M. Bayer, V. Wattelet-Boyer, Bio-Protocol 14 (2024).","mla":"LI, ZIQIANG, et al. “Versatile Cloning Strategy for Efficient Multigene Editing in Arabidopsis.” <i>Bio-Protocol</i>, vol. 14, no. 13, e5029, Bio-Protocol, 2024, doi:<a href=\"https://doi.org/10.21769/BioProtoc.5029\">10.21769/BioProtoc.5029</a>."},"month":"07","article_processing_charge":"Yes","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","file_date_updated":"2024-07-16T06:16:11Z","quality_controlled":"1","publication_status":"published","author":[{"last_name":"Li","id":"922e68bb-1727-11ee-857c-966e8cc1b6c3","full_name":"Li, Ziqiang","first_name":"Ziqiang"},{"full_name":"Huard, Jennifer","first_name":"Jennifer","last_name":"Huard"},{"last_name":"Bayer","full_name":"Bayer, Emmanuelle M.","first_name":"Emmanuelle M."},{"full_name":"Wattelet-Boyer, Valérie","first_name":"Valérie","last_name":"Wattelet-Boyer"}],"type":"journal_article","language":[{"iso":"eng"}],"ddc":["570"],"issue":"13","has_accepted_license":"1","status":"public","date_updated":"2026-10-21T07:57:41Z","date_created":"2024-07-14T22:01:11Z","file":[{"content_type":"application/pdf","checksum":"c8671c0ad483da6407cb16cc3fef1990","success":1,"file_name":"2024_BioProtocol_Li.pdf","creator":"dernst","date_created":"2024-07-16T06:16:11Z","relation":"main_file","date_updated":"2024-07-16T06:16:11Z","access_level":"open_access","file_size":2896048,"file_id":"17242"}]},{"status":"public","language":[{"iso":"eng"}],"isi":1,"date_updated":"2026-10-21T07:57:17Z","date_created":"2024-06-30T22:01:05Z","article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"short":"S. Lembo, M.K. Sixt, Nature Immunology 25 (2024) 1131–1132.","ista":"Lembo S, Sixt MK. 2024. Nuclear squeezing wakes up dendritic cells. Nature Immunology. 25, 1131–1132.","mla":"Lembo, Sergio, and Michael K. Sixt. “Nuclear Squeezing Wakes up Dendritic Cells.” <i>Nature Immunology</i>, vol. 25, Springer Nature, 2024, pp. 1131–1132, doi:<a href=\"https://doi.org/10.1038/s41590-024-01881-2\">10.1038/s41590-024-01881-2</a>.","chicago":"Lembo, Sergio, and Michael K Sixt. “Nuclear Squeezing Wakes up Dendritic Cells.” <i>Nature Immunology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41590-024-01881-2\">https://doi.org/10.1038/s41590-024-01881-2</a>.","ama":"Lembo S, Sixt MK. Nuclear squeezing wakes up dendritic cells. <i>Nature Immunology</i>. 2024;25:1131–1132. doi:<a href=\"https://doi.org/10.1038/s41590-024-01881-2\">10.1038/s41590-024-01881-2</a>","ieee":"S. Lembo and M. K. Sixt, “Nuclear squeezing wakes up dendritic cells,” <i>Nature Immunology</i>, vol. 25. Springer Nature, pp. 1131–1132, 2024.","apa":"Lembo, S., &#38; Sixt, M. K. (2024). Nuclear squeezing wakes up dendritic cells. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-024-01881-2\">https://doi.org/10.1038/s41590-024-01881-2</a>"},"oa_version":"None","month":"06","publication_status":"published","author":[{"orcid":"0000-0002-2253-8771","last_name":"Lembo","id":"d993a7b2-292f-11ed-aaac-fb045a912e31","full_name":"Lembo, Sergio","first_name":"Sergio"},{"full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"type":"journal_article","quality_controlled":"1","fulldoi":"https://doi.org/10.1038/s41590-024-01881-2","day":"21","doi":"10.1038/s41590-024-01881-2","das_tickbox":"0","year":"2024","volume":25,"abstract":[{"lang":"eng","text":"Dendritic cells migrate to and from lymph nodes in response to chemokine gradients.Data now show that steady-state migration of these cells can be triggered by a mechanosensitive pathway."}],"supplementarymaterial":"no","external_id":{"isi":["001251509300001"],"pmid":["38907047"]},"publication":"Nature Immunology","page":"1131–1132 ","publisher":"Springer Nature","pmid":1,"corr_author":"1","publication_identifier":{"eissn":["1529-2916"],"issn":["1529-2908"]},"department":[{"_id":"MiSi"}],"_id":"17191","researchdata_availability":"no","title":"Nuclear squeezing wakes up dendritic cells","scopus_import":"1","intvolume":"        25","date_published":"2024-06-21T00:00:00Z","article_type":"letter_note"},{"ddc":["570"],"language":[{"iso":"eng"}],"issue":"9","isi":1,"has_accepted_license":"1","status":"public","date_updated":"2026-10-21T08:01:09Z","date_created":"2024-09-22T22:01:42Z","file":[{"file_id":"18162","file_size":6892750,"access_level":"open_access","date_created":"2024-09-30T09:16:03Z","date_updated":"2024-09-30T09:16:03Z","relation":"main_file","creator":"dernst","file_name":"2024_Immunity_Stark.pdf","success":1,"checksum":"4683de43d06a8fd8e3fc91af4ddc1ba2","content_type":"application/pdf"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"short":"K. Stark, B. Kilani, S. Stockhausen, J. Busse, I. Schubert, T.D. Tran, F.R. Gärtner, A. Leunig, K. Pekayvaz, L. Nicolai, V. Fumagalli, J. Stermann, F. Stephan, C. David, M.B. Müller, B. Heyman, A. Lux, A. Da Palma Guerreiro, L.P. Frenzel, C.Q. Schmidt, A. Dopler, M. Moser, S. Chandraratne, M.L. Von Brühl, M. Lorenz, T. Korff, M. Rudelius, O. Popp, M. Kirchner, P. Mertins, F. Nimmerjahn, M. Iannacone, M. Sperandio, B. Engelmann, A. Verschoor, S. Massberg, Immunity 57 (2024) 2140–2156.","ista":"Stark K, Kilani B, Stockhausen S, Busse J, Schubert I, Tran TD, Gärtner FR, Leunig A, Pekayvaz K, Nicolai L, Fumagalli V, Stermann J, Stephan F, David C, Müller MB, Heyman B, Lux A, Da Palma Guerreiro A, Frenzel LP, Schmidt CQ, Dopler A, Moser M, Chandraratne S, Von Brühl ML, Lorenz M, Korff T, Rudelius M, Popp O, Kirchner M, Mertins P, Nimmerjahn F, Iannacone M, Sperandio M, Engelmann B, Verschoor A, Massberg S. 2024. Antibodies and complement are key drivers of thrombosis. Immunity. 57(9), 2140–2156.","mla":"Stark, Konstantin, et al. “Antibodies and Complement Are Key Drivers of Thrombosis.” <i>Immunity</i>, vol. 57, no. 9, Elsevier, 2024, pp. 2140–56, doi:<a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">10.1016/j.immuni.2024.08.007</a>.","chicago":"Stark, Konstantin, Badr Kilani, Sven Stockhausen, Johanna Busse, Irene Schubert, Thuy Duong Tran, Florian R Gärtner, et al. “Antibodies and Complement Are Key Drivers of Thrombosis.” <i>Immunity</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">https://doi.org/10.1016/j.immuni.2024.08.007</a>.","ieee":"K. Stark <i>et al.</i>, “Antibodies and complement are key drivers of thrombosis,” <i>Immunity</i>, vol. 57, no. 9. Elsevier, pp. 2140–2156, 2024.","apa":"Stark, K., Kilani, B., Stockhausen, S., Busse, J., Schubert, I., Tran, T. D., … Massberg, S. (2024). Antibodies and complement are key drivers of thrombosis. <i>Immunity</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">https://doi.org/10.1016/j.immuni.2024.08.007</a>","ama":"Stark K, Kilani B, Stockhausen S, et al. Antibodies and complement are key drivers of thrombosis. <i>Immunity</i>. 2024;57(9):2140-2156. doi:<a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">10.1016/j.immuni.2024.08.007</a>"},"oa_version":"Published Version","month":"09","article_processing_charge":"Yes (in subscription journal)","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","file_date_updated":"2024-09-30T09:16:03Z","quality_controlled":"1","publication_status":"published","dataavailabilitystatement":"All data reported in this paper will be shared by the lead contact upon request. This paper does not report original codes. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","author":[{"full_name":"Stark, Konstantin","first_name":"Konstantin","last_name":"Stark"},{"first_name":"Badr","full_name":"Kilani, Badr","last_name":"Kilani"},{"full_name":"Stockhausen, Sven","first_name":"Sven","last_name":"Stockhausen"},{"last_name":"Busse","first_name":"Johanna","full_name":"Busse, Johanna"},{"last_name":"Schubert","full_name":"Schubert, Irene","first_name":"Irene"},{"last_name":"Tran","full_name":"Tran, Thuy Duong","first_name":"Thuy Duong"},{"full_name":"Gärtner, Florian R","first_name":"Florian R","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723"},{"first_name":"Alexander","full_name":"Leunig, Alexander","last_name":"Leunig"},{"first_name":"Kami","full_name":"Pekayvaz, Kami","last_name":"Pekayvaz"},{"full_name":"Nicolai, Leo","first_name":"Leo","last_name":"Nicolai"},{"first_name":"Valeria","full_name":"Fumagalli, Valeria","last_name":"Fumagalli"},{"last_name":"Stermann","first_name":"Julia","full_name":"Stermann, Julia"},{"first_name":"Felix","full_name":"Stephan, Felix","last_name":"Stephan"},{"full_name":"David, Christian","first_name":"Christian","last_name":"David"},{"last_name":"Müller","full_name":"Müller, Martin B.","first_name":"Martin B."},{"full_name":"Heyman, Birgitta","first_name":"Birgitta","last_name":"Heyman"},{"full_name":"Lux, Anja","first_name":"Anja","last_name":"Lux"},{"full_name":"Da Palma Guerreiro, Alexandra","first_name":"Alexandra","last_name":"Da Palma Guerreiro"},{"first_name":"Lukas P.","full_name":"Frenzel, Lukas P.","last_name":"Frenzel"},{"full_name":"Schmidt, Christoph Q.","first_name":"Christoph Q.","last_name":"Schmidt"},{"last_name":"Dopler","full_name":"Dopler, Arthur","first_name":"Arthur"},{"last_name":"Moser","full_name":"Moser, Markus","first_name":"Markus"},{"full_name":"Chandraratne, Sue","first_name":"Sue","last_name":"Chandraratne"},{"last_name":"Von Brühl","first_name":"Marie Luise","full_name":"Von Brühl, Marie Luise"},{"full_name":"Lorenz, Michael","first_name":"Michael","last_name":"Lorenz"},{"full_name":"Korff, Thomas","first_name":"Thomas","last_name":"Korff"},{"full_name":"Rudelius, Martina","first_name":"Martina","last_name":"Rudelius"},{"first_name":"Oliver","full_name":"Popp, Oliver","last_name":"Popp"},{"first_name":"Marieluise","full_name":"Kirchner, Marieluise","last_name":"Kirchner"},{"last_name":"Mertins","full_name":"Mertins, Philipp","first_name":"Philipp"},{"full_name":"Nimmerjahn, Falk","first_name":"Falk","last_name":"Nimmerjahn"},{"last_name":"Iannacone","full_name":"Iannacone, Matteo","first_name":"Matteo"},{"full_name":"Sperandio, Markus","first_name":"Markus","last_name":"Sperandio"},{"first_name":"Bernd","full_name":"Engelmann, Bernd","last_name":"Engelmann"},{"first_name":"Admar","full_name":"Verschoor, Admar","last_name":"Verschoor"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"}],"type":"journal_article","volume":57,"abstract":[{"lang":"eng","text":"Venous thromboembolism (VTE) is a common, deadly disease with an increasing incidence despite preventive efforts. Clinical observations have associated elevated antibody concentrations or antibody-based therapies with thrombotic events. However, how antibodies contribute to thrombosis is unknown. Here, we show that reduced blood flow enabled immunoglobulin M (IgM) to bind to FcμR and the polymeric immunoglobulin receptor (pIgR), initiating endothelial activation and platelet recruitment. Subsequently, the procoagulant surface of activated platelets accommodated antigen- and FcγR-independent IgG deposition. This leads to classical complement activation, setting in motion a prothrombotic vicious circle. Key elements of this mechanism were present in humans in the setting of venous stasis as well as in the dysregulated immunothrombosis of COVID-19. This antibody-driven thrombosis can be prevented by pharmacologically targeting complement. Hence, our results uncover antibodies as previously unrecognized central regulators of thrombosis. These findings carry relevance for therapeutic application of antibodies and open innovative avenues to target thrombosis without compromising hemostasis."}],"fulldoi":"https://doi.org/10.1016/j.immuni.2024.08.007","day":"10","acknowledgement":"We thank Michael Carroll (Harvard Medical School, Boston) for providing Ighmtm1Che, C4−/−, and C3−/− mice; Mark Suter (University of Zurich, Zurich) for providing Aicda−/− mice; Marina Botto (Imperial College London, London) for providing C1q−/− and fB−/− mice; Craig Gerard (Harvard Medical School, Boston) for providing C3aR−/− mice; Falk Nimmerjahn (University Erlangen-Nuernberg, Erlangen) for providing Fcgr−/−Fcgr2b−/− mice; Karl Lang (University of Duisburg-Essen, Essen) for providing Fcmr−/− mice; Hans Hengartner and Rolf Zinkernagel (ETH Zurich, Zurich) for providing KL25 mice; Mark Zabel (University Hospital of Zurich, Zurich) for providing CR2−/− mice; Christie Ballantyne (Baylor College of Medicine, Houston) for providing CD11c−/− mice; and Siamon Gordon (University of Oxford, Oxford) for providing CD11b−/− mice. A.V. wishes to thank Michael Grünaug and dedicates this work to Annette, Rita, and Hans.\r\nThis project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. \r\n947611) (K.S.). This study was supported by the Deutsche Forschungsgemeinschaft through the collaborative research center 914 project B02 (K.S. and S.M.), project B04 (A.V.), project A01 (M.M.), project B01 (M.S.), the collaborative research center 1123 project B07 (K.S. and S.M.), the collaborative research center 359 (project A03 [K.S.] and B02 [M.S.]), the international research training group 1911 project B09 (A.V.), the clinical research unit 303 project 7 (A.V.), cluster of excellence 2167 (A.V.), collaborative research center 1526 project 05 (A.V.), the ANR-DFG project JAKPOT (K.S.), LMUexcellent (K.S.), and the Deutsche Zentrum für Herz-Kreislauf-Forschung (PostDoc Grant and partner site project [K.S. and S.M.]). M.I. is supported by the European Research Council (ERC) Advanced Grant 101141363, ERC Proof of Concept Grant 101138728, Italian Association for Cancer Research (AIRC) Grants 19891 and \r\n22737, Italian Ministry for University and Research Grants PE00000007 (INF-ACT) and PRIN \r\n2022FMESXL, Funded Research Agreement from Asher Biotherapeutics, VIR Biotechnology, and BlueJay Therapeutics. V.F. is supported by the Italian Ministry for University and Research Grants PE00000007 (INF-ACT) and Fondazione Prossimo Mio.","doi":"10.1016/j.immuni.2024.08.007","year":"2024","das_tickbox":"1","supplementarymaterial":"yes","page":"2140-2156","external_id":{"isi":["001317438500001"],"pmid":["39226900"]},"publication":"Immunity","publisher":"Elsevier","pmid":1,"_id":"18109","publication_identifier":{"eissn":["1097-4180"]},"oa":1,"department":[{"_id":"MiSi"}],"date_published":"2024-09-10T00:00:00Z","article_type":"original","researchdata_availability":"upon request","scopus_import":"1","title":"Antibodies and complement are key drivers of thrombosis","intvolume":"        57"},{"volume":154,"abstract":[{"text":"Background: IgE-mediated degranulation of mast cells (MCs) provides rapid protection against environmental hazards, including animal venoms. A fraction of tissue-resident MCs intimately associates with blood vessels. These perivascular MCs were reported to extend projections into the vessel lumen and to be the first MCs to acquire intravenously injected IgE, suggesting that IgE loading of MCs depends on their vascular association.\r\nObjective: We sought to elucidate the molecular basis of the MC–blood vessel interaction and to determine its relevance for IgE-mediated immune responses.\r\nMethods: We selectively inactivated the Itgb1 gene, encoding the β1 chain of integrin adhesion molecules (ITGB1), in MCs by conditional gene targeting in mice. We analyzed skin MCs for blood vessel association, surface IgE density, and capability to bind circulating antibody specific for MC surface molecules, as well as in vivo responses to antigen administered via different routes.\r\nResults: Lack of ITGB1 expression severely compromised MC–blood vessel association. ITGB1-deficient MCs showed normal densities of surface IgE but reduced binding of intravenously injected antibodies. While their capacity to degranulate in response to IgE ligation in vivo was unimpaired, anaphylactic responses to antigen circulating in the vasculature were largely abolished.\r\nConclusions: ITGB1-mediated association of MCs with blood vessels is key for MC immune surveillance of blood vessel content, but is dispensable for slow steady-state loading of endogenous IgE onto tissue-resident MCs.","lang":"eng"}],"day":"01","OA_type":"hybrid","acknowledgement":"This work was funded by Deutsche Forschungsgemeinschaft, Germany, grants RO2133/ 9-1 and RO2133/ 9-2 in the setting of FOR2599 and TR156 project C11 (Project-ID 246807620–TRR 156) to A. Roers and Springboard-to-Postdoc grant of the Dresden International Graduate School for Biomedicine and Bioengineering (DIGS-BB), Dresden, Germany, and Fond zur Förderung der Wissenschaftlichen Forschung (FWF), Austria, Hertha Firnberg grant (project number T-1219) to A. Polikarpova.\r\nWe thank Dr Michael Gerlach, Core Facility Cellular Imaging, Faculty of Medicine Carl Gustav Carus, TU Dresden, for expert support of in vivo imaging experiments; Grace Wurigamule for help with 2-photon imaging and flow cytometric analysis of mouse skin; and Christina Hiller, Livia Schulze, Madelaine Rickauer, and Christa Haase for providing expert technical assistance.","fulldoi":"https://doi.org/10.1016/j.jaci.2024.03.022","year":"2024","doi":"10.1016/j.jaci.2024.03.022","das_tickbox":"0","publisher":"Elsevier","publication":"Journal of Allergy and Clinical Immunology","supplementarymaterial":"yes","page":"745-753","external_id":{"pmid":["38636606"],"isi":["001308886700001"]},"pmid":1,"_id":"15408","publication_identifier":{"eissn":["1097-6825"],"issn":["0091-6749"]},"department":[{"_id":"MiSi"}],"oa":1,"article_type":"original","date_published":"2024-09-01T00:00:00Z","researchdata_availability":"no","intvolume":"       154","scopus_import":"1","title":"Integrin β1–mediated mast cell immune-surveillance of blood vessel content","language":[{"iso":"eng"}],"ddc":["570"],"OA_place":"publisher","issue":"3","isi":1,"status":"public","has_accepted_license":"1","date_updated":"2026-10-21T08:01:51Z","date_created":"2024-05-19T22:01:13Z","file":[{"date_updated":"2025-01-13T10:55:28Z","relation":"main_file","date_created":"2025-01-13T10:55:28Z","creator":"dernst","file_name":"2024_JourAllergyClinicalImm_Link.pdf","success":1,"checksum":"6a5af05082e1869d7cad6406fa4eb76c","content_type":"application/pdf","file_id":"18840","file_size":1792425,"access_level":"open_access"}],"tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"oa_version":"Published Version","citation":{"short":"K. Link, L. Muhandes, A. Polikarpova, T. Lämmermann, M.K. Sixt, R. Fässler, A. Roers, Journal of Allergy and Clinical Immunology 154 (2024) 745–753.","ista":"Link K, Muhandes L, Polikarpova A, Lämmermann T, Sixt MK, Fässler R, Roers A. 2024. Integrin β1–mediated mast cell immune-surveillance of blood vessel content. Journal of Allergy and Clinical Immunology. 154(3), 745–753.","mla":"Link, Kristina, et al. “Integrin Β1–Mediated Mast Cell Immune-Surveillance of Blood Vessel Content.” <i>Journal of Allergy and Clinical Immunology</i>, vol. 154, no. 3, Elsevier, 2024, pp. 745–53, doi:<a href=\"https://doi.org/10.1016/j.jaci.2024.03.022\">10.1016/j.jaci.2024.03.022</a>.","chicago":"Link, Kristina, Lina Muhandes, Anastasia Polikarpova, Tim Lämmermann, Michael K Sixt, Reinhard Fässler, and Axel Roers. “Integrin Β1–Mediated Mast Cell Immune-Surveillance of Blood Vessel Content.” <i>Journal of Allergy and Clinical Immunology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.jaci.2024.03.022\">https://doi.org/10.1016/j.jaci.2024.03.022</a>.","ama":"Link K, Muhandes L, Polikarpova A, et al. Integrin β1–mediated mast cell immune-surveillance of blood vessel content. <i>Journal of Allergy and Clinical Immunology</i>. 2024;154(3):745-753. doi:<a href=\"https://doi.org/10.1016/j.jaci.2024.03.022\">10.1016/j.jaci.2024.03.022</a>","apa":"Link, K., Muhandes, L., Polikarpova, A., Lämmermann, T., Sixt, M. K., Fässler, R., &#38; Roers, A. (2024). Integrin β1–mediated mast cell immune-surveillance of blood vessel content. <i>Journal of Allergy and Clinical Immunology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jaci.2024.03.022\">https://doi.org/10.1016/j.jaci.2024.03.022</a>","ieee":"K. Link <i>et al.</i>, “Integrin β1–mediated mast cell immune-surveillance of blood vessel content,” <i>Journal of Allergy and Clinical Immunology</i>, vol. 154, no. 3. Elsevier, pp. 745–753, 2024."},"month":"09","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_processing_charge":"Yes (in subscription journal)","file_date_updated":"2025-01-13T10:55:28Z","quality_controlled":"1","publication_status":"published","type":"journal_article","author":[{"first_name":"Kristina","full_name":"Link, Kristina","last_name":"Link"},{"last_name":"Muhandes","full_name":"Muhandes, Lina","first_name":"Lina"},{"first_name":"Anastasia","full_name":"Polikarpova, Anastasia","last_name":"Polikarpova"},{"full_name":"Lämmermann, Tim","first_name":"Tim","last_name":"Lämmermann"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"first_name":"Reinhard","full_name":"Fässler, Reinhard","last_name":"Fässler"},{"full_name":"Roers, Axel","first_name":"Axel","last_name":"Roers"}]},{"ddc":["570"],"language":[{"iso":"eng"}],"issue":"1","has_accepted_license":"1","status":"public","date_updated":"2026-10-21T08:00:18Z","date_created":"2024-02-04T23:00:53Z","file":[{"file_id":"14941","file_size":9645056,"access_level":"open_access","date_updated":"2024-02-05T12:35:03Z","relation":"main_file","date_created":"2024-02-05T12:35:03Z","file_name":"2023_EmboReports_PimentaMarques.pdf","creator":"dernst","success":1,"checksum":"53c3ef43d9bd6d7bff3ffcf57d763cac","content_type":"application/pdf"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","citation":{"mla":"Pimenta-Marques, Ana, et al. “Ana1/CEP295 Is an Essential Player in the Centrosome Maintenance Program Regulated by Polo Kinase and the PCM.” <i>EMBO Reports</i>, vol. 25, no. 1, Embo Press, 2024, pp. 102–27, doi:<a href=\"https://doi.org/10.1038/s44319-023-00020-6\">10.1038/s44319-023-00020-6</a>.","short":"A. Pimenta-Marques, T. Perestrelo, P. Dos Reis Rodrigues, P. Duarte, A. Ferreira-Silva, M. Lince-Faria, M. Bettencourt-Dias, EMBO Reports 25 (2024) 102–127.","ista":"Pimenta-Marques A, Perestrelo T, Dos Reis Rodrigues P, Duarte P, Ferreira-Silva A, Lince-Faria M, Bettencourt-Dias M. 2024. Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. EMBO Reports. 25(1), 102–127.","apa":"Pimenta-Marques, A., Perestrelo, T., Dos Reis Rodrigues, P., Duarte, P., Ferreira-Silva, A., Lince-Faria, M., &#38; Bettencourt-Dias, M. (2024). Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. <i>EMBO Reports</i>. Embo Press. <a href=\"https://doi.org/10.1038/s44319-023-00020-6\">https://doi.org/10.1038/s44319-023-00020-6</a>","ieee":"A. Pimenta-Marques <i>et al.</i>, “Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM,” <i>EMBO Reports</i>, vol. 25, no. 1. Embo Press, pp. 102–127, 2024.","ama":"Pimenta-Marques A, Perestrelo T, Dos Reis Rodrigues P, et al. Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. <i>EMBO Reports</i>. 2024;25(1):102-127. doi:<a href=\"https://doi.org/10.1038/s44319-023-00020-6\">10.1038/s44319-023-00020-6</a>","chicago":"Pimenta-Marques, Ana, Tania Perestrelo, Patricia Dos Reis Rodrigues, Paulo Duarte, Ana Ferreira-Silva, Mariana Lince-Faria, and Mónica Bettencourt-Dias. “Ana1/CEP295 Is an Essential Player in the Centrosome Maintenance Program Regulated by Polo Kinase and the PCM.” <i>EMBO Reports</i>. Embo Press, 2024. <a href=\"https://doi.org/10.1038/s44319-023-00020-6\">https://doi.org/10.1038/s44319-023-00020-6</a>."},"month":"01","article_processing_charge":"Yes (in subscription journal)","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","file_date_updated":"2024-02-05T12:35:03Z","quality_controlled":"1","publication_status":"published","dataavailabilitystatement":"This study includes no data deposited in external repositories.","author":[{"last_name":"Pimenta-Marques","full_name":"Pimenta-Marques, Ana","first_name":"Ana"},{"last_name":"Perestrelo","first_name":"Tania","full_name":"Perestrelo, Tania"},{"first_name":"Patricia","full_name":"Dos Reis Rodrigues, Patricia","orcid":"0000-0003-1681-508X","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","last_name":"Dos Reis Rodrigues"},{"full_name":"Duarte, Paulo","first_name":"Paulo","last_name":"Duarte"},{"full_name":"Ferreira-Silva, Ana","first_name":"Ana","last_name":"Ferreira-Silva"},{"last_name":"Lince-Faria","first_name":"Mariana","full_name":"Lince-Faria, Mariana"},{"first_name":"Mónica","full_name":"Bettencourt-Dias, Mónica","last_name":"Bettencourt-Dias"}],"type":"journal_article","volume":25,"abstract":[{"lang":"eng","text":"Centrioles are part of centrosomes and cilia, which are microtubule organising centres (MTOC) with diverse functions. Despite their stability, centrioles can disappear during differentiation, such as in oocytes, but little is known about the regulation of their structural integrity. Our previous research revealed that the pericentriolar material (PCM) that surrounds centrioles and its recruiter, Polo kinase, are downregulated in oogenesis and sufficient for maintaining both centrosome structural integrity and MTOC activity. We now show that the expression of specific components of the centriole cartwheel and wall, including ANA1/CEP295, is essential for maintaining centrosome integrity. We find that Polo kinase requires ANA1 to promote centriole stability in cultured cells and eggs. In addition, ANA1 expression prevents the loss of centrioles observed upon PCM-downregulation. However, the centrioles maintained by overexpressing and tethering ANA1 are inactive, unlike the MTOCs observed upon tethering Polo kinase. These findings demonstrate that several centriole components are needed to maintain centrosome structure. Our study also highlights that centrioles are more dynamic than previously believed, with their structural stability relying on the continuous expression of multiple components."}],"fulldoi":"https://doi.org/10.1038/s44319-023-00020-6","acknowledgement":"We thank all members of the Cell Cycle and Regulation Lab for the discussions and for the critical reading of the manuscript. We thank Tomer Avidor-Reiss (University of Toledo, Toledo, OH), Daniel St. Johnston (The Gurdon Institute, Cambridge, UK), David Glover (University of Cambridge, Cambridge, UK), Jingyan Fu (Agricultural University, Beijing, China) Jordan Raff (University of Oxford, Oxford, UK) and Timothy Megraw (Florida State University, Tallahassee, FL) for sharing tools. We acknowledge the technical support of Instituto Gulbenkian de Ciência (IGC)‘s Advanced Imaging Facility, in particular Gabriel Martins, Nuno Pimpão Martins and José Marques. We also thank Tiago Paixão from the IGC’s Quantitative & Digital Science Unit and Marco Louro from the CCR lab for the support provided on statistical analysis. IGC’s Advanced Imaging Facility (AIF-UIC) is supported by the national Portuguese funding ref# PPBI-POCI-01-0145-FEDER -022122. We thank the IGC’s Fly Facility, supported by CONGENTO (LISBOA-01-0145-FEDER-022170). This work was supported by an ERC grant (ERC-2015-CoG-683258) awarded to MBD and a grant from the Portuguese Research Council (FCT) awarded to APM (PTDC/BIA-BID/32225/2017).","day":"10","das_tickbox":"1","doi":"10.1038/s44319-023-00020-6","year":"2024","publication":"EMBO Reports","supplementarymaterial":"yes","page":"102-127","external_id":{"pmid":["38200359"]},"publisher":"Embo Press","pmid":1,"_id":"14933","publication_identifier":{"eissn":["1469-3178"]},"department":[{"_id":"MiSi"}],"oa":1,"date_published":"2024-01-10T00:00:00Z","article_type":"original","researchdata_availability":"not applicable","scopus_import":"1","title":"Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM","intvolume":"        25"},{"article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"07","oa_version":"None","citation":{"chicago":"Avellaneda Sarrió, Mario, and Michael K Sixt. “Rescuing T Cells from Stiff Tumors.” <i>Cell Chemical Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.chembiol.2024.06.011\">https://doi.org/10.1016/j.chembiol.2024.06.011</a>.","ama":"Avellaneda Sarrió M, Sixt MK. Rescuing T cells from stiff tumors. <i>Cell Chemical Biology</i>. 2024;31(7):1242-1243. doi:<a href=\"https://doi.org/10.1016/j.chembiol.2024.06.011\">10.1016/j.chembiol.2024.06.011</a>","apa":"Avellaneda Sarrió, M., &#38; Sixt, M. K. (2024). Rescuing T cells from stiff tumors. <i>Cell Chemical Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.chembiol.2024.06.011\">https://doi.org/10.1016/j.chembiol.2024.06.011</a>","ieee":"M. Avellaneda Sarrió and M. K. Sixt, “Rescuing T cells from stiff tumors,” <i>Cell Chemical Biology</i>, vol. 31, no. 7. Elsevier, pp. 1242–1243, 2024.","ista":"Avellaneda Sarrió M, Sixt MK. 2024. Rescuing T cells from stiff tumors. Cell Chemical Biology. 31(7), 1242–1243.","short":"M. Avellaneda Sarrió, M.K. Sixt, Cell Chemical Biology 31 (2024) 1242–1243.","mla":"Avellaneda Sarrió, Mario, and Michael K. Sixt. “Rescuing T Cells from Stiff Tumors.” <i>Cell Chemical Biology</i>, vol. 31, no. 7, Elsevier, 2024, pp. 1242–43, doi:<a href=\"https://doi.org/10.1016/j.chembiol.2024.06.011\">10.1016/j.chembiol.2024.06.011</a>."},"author":[{"id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","last_name":"Avellaneda Sarrió","orcid":"0000-0001-6406-524X","first_name":"Mario","full_name":"Avellaneda Sarrió, Mario"},{"first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"type":"journal_article","publication_status":"published","quality_controlled":"1","status":"public","isi":1,"issue":"7","language":[{"iso":"eng"}],"date_updated":"2026-10-21T07:58:00Z","date_created":"2024-07-21T22:01:00Z","department":[{"_id":"MiSi"}],"corr_author":"1","publication_identifier":{"eissn":["2451-9448"],"issn":["2451-9456"]},"_id":"17279","title":"Rescuing T cells from stiff tumors","scopus_import":"1","intvolume":"        31","researchdata_availability":"no","date_published":"2024-07-18T00:00:00Z","article_type":"review","year":"2024","das_tickbox":"0","doi":"10.1016/j.chembiol.2024.06.011","fulldoi":"https://doi.org/10.1016/j.chembiol.2024.06.011","day":"18","abstract":[{"text":"In a recent issue of Cell, Zhang et al.1 demonstrate that mechanical features of a solid tumor can drive T cells into dysfunctionality and identify pathways that revert this “exhausted” state.","lang":"eng"}],"volume":31,"pmid":1,"publication":"Cell Chemical Biology","page":"1242-1243","supplementarymaterial":"no","external_id":{"isi":["001275725000001"],"pmid":["39029454"]},"publisher":"Elsevier"},{"type":"book_chapter","author":[{"full_name":"Leithner, Alexander F","first_name":"Alexander F","last_name":"Leithner","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1073-744X"},{"orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","first_name":"Jack","full_name":"Merrin, Jack"},{"first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt"}],"alternative_title":["Methods in Molecular Biology"],"publication_status":"published","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","month":"04","oa_version":"None","citation":{"ista":"Leithner AF, Merrin J, Sixt MK. 2023.En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses. In: The Immune Synapse. Methods in Molecular Biology, vol. 2654, 137–147.","short":"A.F. Leithner, J. Merrin, M.K. Sixt, in:, C. Baldari, M. Dustin (Eds.), The Immune Synapse, Springer Nature, New York, NY, 2023, pp. 137–147.","mla":"Leithner, Alexander F., et al. “En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses.” <i>The Immune Synapse</i>, edited by Cosima Baldari and Michael Dustin, vol. 2654, Springer Nature, 2023, pp. 137–47, doi:<a href=\"https://doi.org/10.1007/978-1-0716-3135-5_9\">10.1007/978-1-0716-3135-5_9</a>.","chicago":"Leithner, Alexander F, Jack Merrin, and Michael K Sixt. “En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses.” In <i>The Immune Synapse</i>, edited by Cosima Baldari and Michael Dustin, 2654:137–47. MIMB. New York, NY: Springer Nature, 2023. <a href=\"https://doi.org/10.1007/978-1-0716-3135-5_9\">https://doi.org/10.1007/978-1-0716-3135-5_9</a>.","ama":"Leithner AF, Merrin J, Sixt MK. En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses. In: Baldari C, Dustin M, eds. <i>The Immune Synapse</i>. Vol 2654. MIMB. New York, NY: Springer Nature; 2023:137-147. doi:<a href=\"https://doi.org/10.1007/978-1-0716-3135-5_9\">10.1007/978-1-0716-3135-5_9</a>","apa":"Leithner, A. F., Merrin, J., &#38; Sixt, M. K. (2023). En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses. In C. Baldari &#38; M. Dustin (Eds.), <i>The Immune Synapse</i> (Vol. 2654, pp. 137–147). New York, NY: Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-3135-5_9\">https://doi.org/10.1007/978-1-0716-3135-5_9</a>","ieee":"A. F. Leithner, J. Merrin, and M. K. Sixt, “En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses,” in <i>The Immune Synapse</i>, vol. 2654, C. Baldari and M. Dustin, Eds. New York, NY: Springer Nature, 2023, pp. 137–147."},"date_created":"2023-05-22T08:41:48Z","date_updated":"2025-04-14T07:42:07Z","status":"public","acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"},{"_id":"M-Shop"}],"language":[{"iso":"eng"}],"intvolume":"      2654","scopus_import":"1","title":"En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses","date_published":"2023-04-28T00:00:00Z","department":[{"_id":"MiSi"},{"_id":"NanoFab"}],"editor":[{"first_name":"Cosima","full_name":"Baldari, Cosima","last_name":"Baldari"},{"last_name":"Dustin","first_name":"Michael","full_name":"Dustin, Michael"}],"publication_identifier":{"eisbn":["9781071631355"],"isbn":["9781071631348"],"eissn":["1940-6029"],"issn":["1064-3745"]},"_id":"13052","pmid":1,"publisher":"Springer Nature","external_id":{"pmid":["37106180"]},"page":"137-147","publication":"The Immune Synapse","year":"2023","series_title":"MIMB","doi":"10.1007/978-1-0716-3135-5_9","acknowledgement":"A.L. was funded by an Erwin Schrödinger postdoctoral fellowship of the Austrian Science Fund (FWF, project number: J4542-B) and is an EMBO non-stipendiary postdoctoral fellow. This work was supported by a European Research Council grant ERC-CoG-72437 to M.S. We thank the Imaging & Optics facility, the Nanofabrication facility, and the Miba Machine Shop of ISTA for their excellent support.","day":"28","fulldoi":"https://doi.org/10.1007/978-1-0716-3135-5_9","abstract":[{"text":"Imaging of the immunological synapse (IS) between dendritic cells (DCs) and T cells in suspension is hampered by suboptimal alignment of cell-cell contacts along the vertical imaging plane. This requires optical sectioning that often results in unsatisfactory resolution in time and space. Here, we present a workflow where DCs and T cells are confined between a layer of glass and polydimethylsiloxane (PDMS) that orients the cells along one, horizontal imaging plane, allowing for fast en-face-imaging of the DC-T cell IS.","lang":"eng"}],"ec_funded":1,"project":[{"name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"724373"}],"place":"New York, NY","volume":2654},{"corr_author":"1","publication_identifier":{"eissn":["2041-1723"]},"oa":1,"department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"BjHo"}],"_id":"14361","scopus_import":"1","title":"Synchronization in collectively moving inanimate and living active matter","intvolume":"        14","article_number":"5633","date_published":"2023-09-13T00:00:00Z","article_type":"original","fulldoi":"https://doi.org/10.1038/s41467-023-41432-1","acknowledgement":"We thank K. O’Keeffe, E. Hannezo, P. Devreotes, C. Dessalles, and E. Martens for discussion and/or critical reading of the manuscript; the Bioimaging Facility of ISTA for excellent support, as well as the Life Science Facility and the Miba Machine Shop of ISTA. This work was supported by the European Research Council (ERC StG 281556 and CoG 724373) to M.S.","day":"13","year":"2023","doi":"10.1038/s41467-023-41432-1","volume":14,"project":[{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes","_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"281556"},{"name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"724373"}],"ec_funded":1,"abstract":[{"text":"Whether one considers swarming insects, flocking birds, or bacterial colonies, collective motion arises from the coordination of individuals and entails the adjustment of their respective velocities. In particular, in close confinements, such as those encountered by dense cell populations during development or regeneration, collective migration can only arise coordinately. Yet, how individuals unify their velocities is often not understood. Focusing on a finite number of cells in circular confinements, we identify waves of polymerizing actin that function as a pacemaker governing the speed of individual cells. We show that the onset of collective motion coincides with the synchronization of the wave nucleation frequencies across the population. Employing a simpler and more readily accessible mechanical model system of active spheres, we identify the synchronization of the individuals’ internal oscillators as one of the essential requirements to reach the corresponding collective state. The mechanical ‘toy’ experiment illustrates that the global synchronous state is achieved by nearest neighbor coupling. We suggest by analogy that local coupling and the synchronization of actin waves are essential for the emergent, self-organized motion of cell collectives.","lang":"eng"}],"external_id":{"pmid":["37704595"],"isi":["001087583700030"]},"publication":"Nature Communications","publisher":"Springer Nature","pmid":1,"article_processing_charge":"Yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","citation":{"ista":"Riedl M, Mayer ID, Merrin J, Sixt MK, Hof B. 2023. Synchronization in collectively moving inanimate and living active matter. Nature Communications. 14, 5633.","short":"M. Riedl, I.D. Mayer, J. Merrin, M.K. Sixt, B. Hof, Nature Communications 14 (2023).","mla":"Riedl, Michael, et al. “Synchronization in Collectively Moving Inanimate and Living Active Matter.” <i>Nature Communications</i>, vol. 14, 5633, Springer Nature, 2023, doi:<a href=\"https://doi.org/10.1038/s41467-023-41432-1\">10.1038/s41467-023-41432-1</a>.","chicago":"Riedl, Michael, Isabelle D Mayer, Jack Merrin, Michael K Sixt, and Björn Hof. “Synchronization in Collectively Moving Inanimate and Living Active Matter.” <i>Nature Communications</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41467-023-41432-1\">https://doi.org/10.1038/s41467-023-41432-1</a>.","ieee":"M. Riedl, I. D. Mayer, J. Merrin, M. K. Sixt, and B. Hof, “Synchronization in collectively moving inanimate and living active matter,” <i>Nature Communications</i>, vol. 14. Springer Nature, 2023.","apa":"Riedl, M., Mayer, I. D., Merrin, J., Sixt, M. K., &#38; Hof, B. (2023). Synchronization in collectively moving inanimate and living active matter. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-023-41432-1\">https://doi.org/10.1038/s41467-023-41432-1</a>","ama":"Riedl M, Mayer ID, Merrin J, Sixt MK, Hof B. Synchronization in collectively moving inanimate and living active matter. <i>Nature Communications</i>. 2023;14. doi:<a href=\"https://doi.org/10.1038/s41467-023-41432-1\">10.1038/s41467-023-41432-1</a>"},"month":"09","publication_status":"published","author":[{"first_name":"Michael","full_name":"Riedl, Michael","orcid":"0000-0003-4844-6311","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","last_name":"Riedl"},{"first_name":"Isabelle D","full_name":"Mayer, Isabelle D","id":"61763940-15b2-11ec-abd3-cfaddfbc66b4","last_name":"Mayer"},{"orcid":"0000-0001-5145-4609","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","first_name":"Jack"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","first_name":"Michael K"},{"full_name":"Hof, Björn","first_name":"Björn","last_name":"Hof","id":"3A374330-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2057-2754"}],"type":"journal_article","file_date_updated":"2023-09-25T08:32:37Z","quality_controlled":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"has_accepted_license":"1","status":"public","ddc":["530","570"],"language":[{"iso":"eng"}],"isi":1,"file":[{"file_size":2317272,"access_level":"open_access","file_id":"14366","checksum":"82d2d4ad736cc8493db8ce45cd313f7b","content_type":"application/pdf","relation":"main_file","date_updated":"2023-09-25T08:32:37Z","date_created":"2023-09-25T08:32:37Z","creator":"dernst","file_name":"2023_NatureComm_Riedl.pdf","success":1}],"date_created":"2023-09-24T22:01:10Z","date_updated":"2025-04-14T13:10:03Z"},{"date_published":"2023-11-16T00:00:00Z","title":"Synchronization in collectively moving active matter","_id":"14530","publication_identifier":{"issn":["2663-337X"]},"corr_author":"1","oa":1,"department":[{"_id":"GradSch"},{"_id":"MiSi"}],"publisher":"Institute of Science and Technology Austria","page":"260","abstract":[{"lang":"eng","text":"Most motions of many-body systems at any scale in nature with sufficient degrees of freedom tend to be chaotic; reaching from the orbital motion of planets, the air currents in our atmosphere, down to the water flowing through our pipelines or the movement of a population of bacteria. To the observer it is therefore intriguing when a moving collective exhibits order. Collective motion of flocks of birds, schools of fish or swarms of self-propelled particles or robots have been studied extensively over the past decades but the mechanisms involved in the transition from chaos to order remain unclear. Here, the interactions, that in most systems give rise to chaos, sustain order.  In this thesis we investigate mechanisms that preserve, destabilize or lead to the ordered state. We show that endothelial cells migrating in circular confinements transition to a collective rotating state and concomitantly synchronize the frequencies of nucleating actin waves within individual cells. Consequently, the frequency dependent cell migration speed uniformizes across the population. Complementary to the WAVE dependent nucleation of traveling actin waves, we show that in leukocytes the actin polymerization depending on WASp generates pushing forces locally at stationary patches. Next, in pipe flows, we study methods to disrupt the self--sustaining cycle of turbulence and therefore relaminarize the flow. While we find in pulsating flow conditions that turbulence emerges through a helical instability during the decelerating phase. Finally, we show quantitatively in brain slices of mice that wild-type control neurons can compensate the migratory deficits of a genetically modified neuronal sub--population in the developing cortex.  "}],"day":"16","keyword":["Synchronization","Collective Movement","Active Matter","Cell Migration","Active Colloids"],"fulldoi":"https://doi.org/10.15479/14530","year":"2023","doi":"10.15479/14530","file_date_updated":"2023-11-15T09:52:54Z","alternative_title":["ISTA Thesis"],"supervisor":[{"first_name":"Björn","full_name":"Hof, Björn","id":"3A374330-F248-11E8-B48F-1D18A9856A87","last_name":"Hof","orcid":"0000-0003-2057-2754"}],"publication_status":"published","type":"dissertation","author":[{"full_name":"Riedl, Michael","first_name":"Michael","orcid":"0000-0003-4844-6311","last_name":"Riedl","id":"3BE60946-F248-11E8-B48F-1D18A9856A87"}],"citation":{"short":"M. Riedl, Synchronization in Collectively Moving Active Matter, Institute of Science and Technology Austria, 2023.","ista":"Riedl M. 2023. Synchronization in collectively moving active matter. Institute of Science and Technology Austria.","mla":"Riedl, Michael. <i>Synchronization in Collectively Moving Active Matter</i>. Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/14530\">10.15479/14530</a>.","chicago":"Riedl, Michael. “Synchronization in Collectively Moving Active Matter.” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/14530\">https://doi.org/10.15479/14530</a>.","ama":"Riedl M. Synchronization in collectively moving active matter. 2023. doi:<a href=\"https://doi.org/10.15479/14530\">10.15479/14530</a>","apa":"Riedl, M. (2023). <i>Synchronization in collectively moving active matter</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/14530\">https://doi.org/10.15479/14530</a>","ieee":"M. Riedl, “Synchronization in collectively moving active matter,” Institute of Science and Technology Austria, 2023."},"oa_version":"Updated Version","month":"11","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_processing_charge":"No","date_created":"2023-11-15T09:59:03Z","date_updated":"2026-04-07T13:29:13Z","file":[{"creator":"mriedl","file_name":"Thesis_Riedl_2023_corr.pdf","success":1,"relation":"main_file","date_updated":"2023-11-15T09:52:54Z","date_created":"2023-11-15T09:52:54Z","content_type":"application/pdf","checksum":"52e1d0ab6c1abe59c82dfe8c9ff5f83a","file_id":"14536","file_size":36743942,"access_level":"open_access"}],"language":[{"iso":"eng"}],"OA_place":"publisher","related_material":{"record":[{"id":"461","relation":"part_of_dissertation","status":"public"},{"relation":"part_of_dissertation","id":"10791","status":"public"},{"relation":"part_of_dissertation","id":"7932","status":"public"},{"relation":"part_of_dissertation","id":"10703","status":"public"},{"status":"public","id":"12726","relation":"old_edition"}]},"ddc":["530","570"],"degree_awarded":"PhD","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"status":"public","has_accepted_license":"1"},{"_id":"14555","publication_identifier":{"eissn":["2296-634X"]},"corr_author":"1","department":[{"_id":"MiSi"}],"oa":1,"article_type":"original","date_published":"2023-10-31T00:00:00Z","article_number":"1287420","intvolume":"        11","scopus_import":"1","title":"The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction","volume":11,"abstract":[{"text":"The intricate regulatory processes behind actin polymerization play a crucial role in cellular biology, including essential mechanisms such as cell migration or cell division. However, the self-organizing principles governing actin polymerization are still poorly understood. In this perspective article, we compare the Belousov-Zhabotinsky (BZ) reaction, a classic and well understood chemical oscillator known for its self-organizing spatiotemporal dynamics, with the excitable dynamics of polymerizing actin. While the BZ reaction originates from the domain of inorganic chemistry, it shares remarkable similarities with actin polymerization, including the characteristic propagating waves, which are influenced by geometry and external fields, and the emergent collective behavior. Starting with a general description of emerging patterns, we elaborate on single droplets or cell-level dynamics, the influence of geometric confinements and conclude with collective interactions. Comparing these two systems sheds light on the universal nature of self-organization principles in both living and inanimate systems.","lang":"eng"}],"acknowledgement":"The author(s) declare that no financial support was received for the research, authorship, and/or publication of this article.","day":"31","fulldoi":"https://doi.org/10.3389/fcell.2023.1287420","year":"2023","doi":"10.3389/fcell.2023.1287420","publisher":"Frontiers","external_id":{"isi":["001100762800001"],"pmid":["38020899"]},"publication":"Frontiers in Cell and Developmental Biology","pmid":1,"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"mla":"Riedl, Michael, and Michael K. Sixt. “The Excitable Nature of Polymerizing Actin and the Belousov-Zhabotinsky Reaction.” <i>Frontiers in Cell and Developmental Biology</i>, vol. 11, 1287420, Frontiers, 2023, doi:<a href=\"https://doi.org/10.3389/fcell.2023.1287420\">10.3389/fcell.2023.1287420</a>.","ista":"Riedl M, Sixt MK. 2023. The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction. Frontiers in Cell and Developmental Biology. 11, 1287420.","short":"M. Riedl, M.K. Sixt, Frontiers in Cell and Developmental Biology 11 (2023).","ieee":"M. Riedl and M. K. Sixt, “The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction,” <i>Frontiers in Cell and Developmental Biology</i>, vol. 11. Frontiers, 2023.","apa":"Riedl, M., &#38; Sixt, M. K. (2023). The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction. <i>Frontiers in Cell and Developmental Biology</i>. Frontiers. <a href=\"https://doi.org/10.3389/fcell.2023.1287420\">https://doi.org/10.3389/fcell.2023.1287420</a>","ama":"Riedl M, Sixt MK. The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction. <i>Frontiers in Cell and Developmental Biology</i>. 2023;11. doi:<a href=\"https://doi.org/10.3389/fcell.2023.1287420\">10.3389/fcell.2023.1287420</a>","chicago":"Riedl, Michael, and Michael K Sixt. “The Excitable Nature of Polymerizing Actin and the Belousov-Zhabotinsky Reaction.” <i>Frontiers in Cell and Developmental Biology</i>. Frontiers, 2023. <a href=\"https://doi.org/10.3389/fcell.2023.1287420\">https://doi.org/10.3389/fcell.2023.1287420</a>."},"month":"10","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_processing_charge":"Yes","file_date_updated":"2023-11-20T08:41:15Z","quality_controlled":"1","publication_status":"published","type":"journal_article","author":[{"orcid":"0000-0003-4844-6311","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","last_name":"Riedl","first_name":"Michael","full_name":"Riedl, Michael"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K"}],"ddc":["570"],"language":[{"iso":"eng"}],"isi":1,"status":"public","has_accepted_license":"1","date_updated":"2025-09-09T13:22:00Z","date_created":"2023-11-19T23:00:55Z","file":[{"checksum":"61857fc3ebf019354932e7ee684658ce","content_type":"application/pdf","relation":"main_file","date_created":"2023-11-20T08:41:15Z","date_updated":"2023-11-20T08:41:15Z","creator":"dernst","file_name":"2023_FrontiersCellDevBio_Riedl.pdf","success":1,"file_size":2047622,"access_level":"open_access","file_id":"14561"}]},{"date_published":"2023-11-01T00:00:00Z","quality_controlled":"1","alternative_title":["New Developments in NMR"],"publication_status":"published","author":[{"last_name":"Wruck","full_name":"Wruck, F.","first_name":"F."},{"full_name":"Avellaneda Sarrió, Mario","first_name":"Mario","last_name":"Avellaneda Sarrió","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","orcid":"0000-0001-6406-524X"},{"first_name":"M. M.","full_name":"Naqvi, M. M.","last_name":"Naqvi"},{"last_name":"Koers","first_name":"E. J.","full_name":"Koers, E. J."},{"last_name":"Till","first_name":"K.","full_name":"Till, K."},{"full_name":"Gross, L.","first_name":"L.","last_name":"Gross"},{"last_name":"Moayed","first_name":"F.","full_name":"Moayed, F."},{"last_name":"Roland","first_name":"A.","full_name":"Roland, A."},{"last_name":"Heling","first_name":"L. W. H. J.","full_name":"Heling, L. W. H. J."},{"last_name":"Mashaghi","full_name":"Mashaghi, A.","first_name":"A."},{"last_name":"Tans","full_name":"Tans, S. J.","first_name":"S. J."}],"title":"Probing Single Chaperone Substrates","type":"book_chapter","intvolume":"        29","citation":{"apa":"Wruck, F., Avellaneda Sarrió, M., Naqvi, M. M., Koers, E. J., Till, K., Gross, L., … Tans, S. J. (2023). Probing Single Chaperone Substrates. In S. Hiller, M. Liu, &#38; L. He (Eds.), <i>Biophysics of Molecular Chaperones</i> (Vol. 29, pp. 278–318). Royal Society of Chemistry. <a href=\"https://doi.org/10.1039/bk9781839165986-00278\">https://doi.org/10.1039/bk9781839165986-00278</a>","ieee":"F. Wruck <i>et al.</i>, “Probing Single Chaperone Substrates,” in <i>Biophysics of Molecular Chaperones</i>, vol. 29, S. Hiller, M. Liu, and L. He, Eds. Royal Society of Chemistry, 2023, pp. 278–318.","ama":"Wruck F, Avellaneda Sarrió M, Naqvi MM, et al. Probing Single Chaperone Substrates. In: Hiller S, Liu M, He L, eds. <i>Biophysics of Molecular Chaperones</i>. Vol 29. Royal Society of Chemistry; 2023:278-318. doi:<a href=\"https://doi.org/10.1039/bk9781839165986-00278\">10.1039/bk9781839165986-00278</a>","chicago":"Wruck, F., Mario Avellaneda Sarrió, M. M. Naqvi, E. J. Koers, K. Till, L. Gross, F. Moayed, et al. “Probing Single Chaperone Substrates.” In <i>Biophysics of Molecular Chaperones</i>, edited by Sebastian Hiller, Maili Liu, and Lichun He, 29:278–318. Royal Society of Chemistry, 2023. <a href=\"https://doi.org/10.1039/bk9781839165986-00278\">https://doi.org/10.1039/bk9781839165986-00278</a>.","mla":"Wruck, F., et al. “Probing Single Chaperone Substrates.” <i>Biophysics of Molecular Chaperones</i>, edited by Sebastian Hiller et al., vol. 29, Royal Society of Chemistry, 2023, pp. 278–318, doi:<a href=\"https://doi.org/10.1039/bk9781839165986-00278\">10.1039/bk9781839165986-00278</a>.","ista":"Wruck F, Avellaneda Sarrió M, Naqvi MM, Koers EJ, Till K, Gross L, Moayed F, Roland A, Heling LWHJ, Mashaghi A, Tans SJ. 2023.Probing Single Chaperone Substrates. In: Biophysics of Molecular Chaperones. New Developments in NMR, vol. 29, 278–318.","short":"F. Wruck, M. Avellaneda Sarrió, M.M. Naqvi, E.J. Koers, K. Till, L. Gross, F. Moayed, A. Roland, L.W.H.J. Heling, A. Mashaghi, S.J. Tans, in:, S. Hiller, M. Liu, L. He (Eds.), Biophysics of Molecular Chaperones, Royal Society of Chemistry, 2023, pp. 278–318."},"oa_version":"None","_id":"14848","month":"11","article_processing_charge":"No","publication_identifier":{"isbn":["9781839162824"],"eisbn":["9781839165993"]},"editor":[{"last_name":"Hiller","first_name":"Sebastian","full_name":"Hiller, Sebastian"},{"last_name":"Liu","full_name":"Liu, Maili","first_name":"Maili"},{"last_name":"He","full_name":"He, Lichun","first_name":"Lichun"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"MiSi"}],"publication":"Biophysics of Molecular Chaperones","date_updated":"2024-01-23T12:01:53Z","page":"278-318","date_created":"2024-01-22T08:07:02Z","publisher":"Royal Society of Chemistry","volume":29,"language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"Regulating protein states is considered the core function of chaperones. However, despite their importance to all major cellular processes, the conformational changes that chaperones impart on polypeptide chains are difficult to study directly due to their heterogeneous, dynamic, and multi-step nature. Here, we review recent advances towards this aim using single-molecule manipulation methods, which are rapidly revealing new mechanisms of conformational control and helping to define a different perspective on the chaperone function."}],"fulldoi":"https://doi.org/10.1039/bk9781839165986-00278","day":"01","doi":"10.1039/bk9781839165986-00278","year":"2023","status":"public"},{"file":[{"embargo":"2024-12-20","content_type":"application/pdf","checksum":"457927165d5d556305d3086f6b83e5c7","file_name":"Thesis.pdf","creator":"jstopp","relation":"main_file","date_updated":"2024-12-20T23:30:04Z","date_created":"2023-12-20T09:35:34Z","access_level":"open_access","file_size":51585778,"file_id":"14699"},{"date_created":"2023-12-20T09:35:35Z","date_updated":"2024-12-20T23:30:04Z","relation":"source_file","file_name":"Thesis.docx","creator":"jstopp","checksum":"e8d26449ac461f5e8478a62c9507506f","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_id":"14700","access_level":"closed","embargo_to":"open_access","file_size":69625950}],"date_created":"2023-12-18T19:14:28Z","date_updated":"2026-06-18T17:34:48Z","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"related_material":{"record":[{"relation":"part_of_dissertation","id":"14360","status":"public"},{"relation":"part_of_dissertation","id":"12272","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"6328"},{"status":"public","relation":"part_of_dissertation","id":"7885"},{"status":"public","id":"14274","relation":"part_of_dissertation"}]},"OA_place":"publisher","ddc":["570"],"degree_awarded":"PhD","alternative_title":["ISTA Thesis"],"supervisor":[{"first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"publication_status":"published","type":"dissertation","author":[{"id":"489E3F00-F248-11E8-B48F-1D18A9856A87","last_name":"Stopp","first_name":"Julian A","full_name":"Stopp, Julian A"}],"file_date_updated":"2024-12-20T23:30:04Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_processing_charge":"No","citation":{"ista":"Stopp JA. 2023. Neutrophils on the hunt : Migratory strategies employed by neutrophils to fulfill their effector function. Institute of Science and Technology Austria.","short":"J.A. Stopp, Neutrophils on the Hunt : Migratory Strategies Employed by Neutrophils to Fulfill Their Effector Function, Institute of Science and Technology Austria, 2023.","mla":"Stopp, Julian A. <i>Neutrophils on the Hunt : Migratory Strategies Employed by Neutrophils to Fulfill Their Effector Function</i>. Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/at:ista:14697\">10.15479/at:ista:14697</a>.","chicago":"Stopp, Julian A. “Neutrophils on the Hunt : Migratory Strategies Employed by Neutrophils to Fulfill Their Effector Function.” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/at:ista:14697\">https://doi.org/10.15479/at:ista:14697</a>.","ieee":"J. A. Stopp, “Neutrophils on the hunt : Migratory strategies employed by neutrophils to fulfill their effector function,” Institute of Science and Technology Austria, 2023.","apa":"Stopp, J. A. (2023). <i>Neutrophils on the hunt : Migratory strategies employed by neutrophils to fulfill their effector function</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:14697\">https://doi.org/10.15479/at:ista:14697</a>","ama":"Stopp JA. Neutrophils on the hunt : Migratory strategies employed by neutrophils to fulfill their effector function. 2023. doi:<a href=\"https://doi.org/10.15479/at:ista:14697\">10.15479/at:ista:14697</a>"},"oa_version":"Published Version","month":"12","publisher":"Institute of Science and Technology Austria","page":"226","day":"20","fulldoi":"https://doi.org/10.15479/at:ista:14697","doi":"10.15479/at:ista:14697","year":"2023","project":[{"call_identifier":"H2020","grant_number":"665385","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"}],"ec_funded":1,"abstract":[{"lang":"eng","text":"During my Ph.D. research, I managed a series of projects, each focused on the\r\nmechanisms underlying cell migration. My work involved an in-depth examination of\r\nthe complex strategies employed by neutrophils, with a specific focus on their ability to\r\nsynchronize spatial-temporal cues and optimize their gradient perception. However, it\r\nis essential to acknowledge that not all projects yielded successful results, as some\r\nideas were discontinued and are archived for future reference within this thesis.\r\nMy main project investigated how neutrophils decode spatial cues for precise navigation. Human neutrophils showcased distinct movement patterns based on source\r\ntype – linear or point-like. By combining single-cell tracking in 3D environments with\r\nproxy dyes, this project linked cell behaviors to gradient changes, revealing a stronger\r\nresponse to semi-exponential gradients from point sources. In addition, neutrophils\r\nexhibited oscillating migration speeds, using speed minima to adjust trajectories toward sources. Experiencing continuous concentration changes, they accelerated over\r\ntime and employed a \"Run and Fumble\" strategy, alternating between consistent runs\r\nand strategic \"tumbles\" for efficient navigation.\r\nThe project extended to the possibility of cells amplifying perceived gradients by\r\nenclosing their immediate surroundings, pushing attractants forward for enrichment\r\nwhile depleting it at the cell rear. Microfluidic devices were employed, and various experimental parameters configurations were optimized. Although significant differences\r\nin migratory efficacy were detected across pore sizes and device heights, quantifying\r\ngradient manipulation effects proved challenging.\r\nThe \"Laser-Assisted Protein Adsorption by Photobleaching\" (LAPAP) project was\r\npromising, as it allowed the printing of gradients. Initially successful with dendritic cells,\r\nwe aimed to adapt it for neutrophils. Through extensive experimentation with multiple\r\nparameters, we attempted to trigger responses from neutrophils. Despite these efforts\r\nand collaboration, the project failed due to practical challenges and limitations.\r\nFacing a lack of neutrophil-like cells at IST, we initially established the SCF-HoxB8\r\nprimary murine cell line. Despite their existence, their migratory behavior was largely\r\nunexplored due to potential limitations. Through differentiation protocol refinements we\r\nenhanced their migratory capabilities, though their capacity still lagged behind human\r\nneutrophils. Despite this, the improved migration potential of these cells pointed toward\r\ntheir utility for in vitro murine neutrophil migration studies."}],"title":"Neutrophils on the hunt : Migratory strategies employed by neutrophils to fulfill their effector function","date_published":"2023-12-20T00:00:00Z","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-038-1"]},"corr_author":"1","department":[{"_id":"GradSch"},{"_id":"MiSi"}],"oa":1,"_id":"14697"},{"publication_identifier":{"eissn":["2041-1723"]},"department":[{"_id":"MiSi"}],"oa":1,"_id":"14360","title":"Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles","scopus_import":"1","intvolume":"        14","article_number":"5644","date_published":"2023-09-13T00:00:00Z","article_type":"original","fulldoi":"https://doi.org/10.1038/s41467-023-41173-1","day":"13","acknowledgement":"We thank Jan Ellenberg, Leanne Strauss, Anusha Gopalan, and Jia Hui Li for critical feedback on the manuscript and the Life Science Editors for editing assistance. The plasmid with hSnx33 was a kind gift from Duanqing Pei. Cell line with GFP-tagged IRSp53 was a kind gift from Orion Weiner. We thank Brian Graziano for providing protocols, reagents, and key advice to generate CRISPR knockout HL-60 cells. We thank the EMBL flow cytometry core facility, the EMBL advanced light microscopy facility, the EMBL proteomics facility, and the EMBL genomics core facility for support and advice. We thank Anusha Gopalan and Martin Bergert for their support during mechanical measurements by AFM. We thank Estela Sosa Osorio for technical assistance for the co-immunoprecipitation. We thank the EMBL genome biology computational support (and specially Charles Girardot and Jelle Scholtalbers) for critical assistance during RNAseq analysis. We thank Hans Kristian Hannibal‐Bach for his technical assistance during the lipidomic analysis of plasma membrane isolates. We thank Steffen Burgold for their support with LLS7 microscope in the ZEISS Microscopy Customer Center Europe. We acknowledge the financial support of the European Molecular Biology Laboratory (EMBL) to A.D.-M., Y.S., A.K., and A.E., the EMBL Interdisciplinary Postdocs (EIPOD) program under Marie Sklodowska-Curie COFUND actions MSCA-COFUND-FP to M.S.B. and M. S. (grant agreement number: 847543), the BEST program funding by FCT (SFRH/BEST/150300/2019) to S.D.A. and the Joachim Herz Stiftung Add-on Fellowship for Interdisciplinary Science to E.S.\r\nOpen Access funding enabled and organized by Projekt DEAL.","doi":"10.1038/s41467-023-41173-1","year":"2023","volume":14,"abstract":[{"text":"To navigate through diverse tissues, migrating cells must balance persistent self-propelled motion with adaptive behaviors to circumvent obstacles. We identify a curvature-sensing mechanism underlying obstacle evasion in immune-like cells. Specifically, we propose that actin polymerization at the advancing edge of migrating cells is inhibited by the curvature-sensitive BAR domain protein Snx33 in regions with inward plasma membrane curvature. The genetic perturbation of this machinery reduces the cells’ capacity to evade obstructions combined with faster and more persistent cell migration in obstacle-free environments. Our results show how cells can read out their surface topography and utilize actin and plasma membrane biophysics to interpret their environment, allowing them to adaptively decide if they should move ahead or turn away. On the basis of our findings, we propose that the natural diversity of BAR domain proteins may allow cells to tune their curvature sensing machinery to match the shape characteristics in their environment.","lang":"eng"}],"publication":"Nature Communications","external_id":{"isi":["001087583700008"],"pmid":["37704612"]},"publisher":"Springer Nature","pmid":1,"article_processing_charge":"Yes (via OA deal)","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"ieee":"E. Sitarska <i>et al.</i>, “Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles,” <i>Nature Communications</i>, vol. 14. Springer Nature, 2023.","apa":"Sitarska, E., Almeida, S. D., Beckwith, M. S., Stopp, J. A., Czuchnowski, J., Siggel, M., … Diz-Muñoz, A. (2023). Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-023-41173-1\">https://doi.org/10.1038/s41467-023-41173-1</a>","ama":"Sitarska E, Almeida SD, Beckwith MS, et al. Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles. <i>Nature Communications</i>. 2023;14. doi:<a href=\"https://doi.org/10.1038/s41467-023-41173-1\">10.1038/s41467-023-41173-1</a>","chicago":"Sitarska, Ewa, Silvia Dias Almeida, Marianne Sandvold Beckwith, Julian A Stopp, Jakub Czuchnowski, Marc Siggel, Rita Roessner, et al. “Sensing Their Plasma Membrane Curvature Allows Migrating Cells to Circumvent Obstacles.” <i>Nature Communications</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41467-023-41173-1\">https://doi.org/10.1038/s41467-023-41173-1</a>.","mla":"Sitarska, Ewa, et al. “Sensing Their Plasma Membrane Curvature Allows Migrating Cells to Circumvent Obstacles.” <i>Nature Communications</i>, vol. 14, 5644, Springer Nature, 2023, doi:<a href=\"https://doi.org/10.1038/s41467-023-41173-1\">10.1038/s41467-023-41173-1</a>.","short":"E. Sitarska, S.D. Almeida, M.S. Beckwith, J.A. Stopp, J. Czuchnowski, M. Siggel, R. Roessner, A. Tschanz, C. Ejsing, Y. Schwab, J. Kosinski, M.K. Sixt, A. Kreshuk, A. Erzberger, A. Diz-Muñoz, Nature Communications 14 (2023).","ista":"Sitarska E, Almeida SD, Beckwith MS, Stopp JA, Czuchnowski J, Siggel M, Roessner R, Tschanz A, Ejsing C, Schwab Y, Kosinski J, Sixt MK, Kreshuk A, Erzberger A, Diz-Muñoz A. 2023. Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles. Nature Communications. 14, 5644."},"oa_version":"Published Version","month":"09","publication_status":"published","author":[{"full_name":"Sitarska, Ewa","first_name":"Ewa","last_name":"Sitarska"},{"first_name":"Silvia Dias","full_name":"Almeida, Silvia Dias","last_name":"Almeida"},{"first_name":"Marianne Sandvold","full_name":"Beckwith, Marianne Sandvold","last_name":"Beckwith"},{"last_name":"Stopp","id":"489E3F00-F248-11E8-B48F-1D18A9856A87","full_name":"Stopp, Julian A","first_name":"Julian A"},{"first_name":"Jakub","full_name":"Czuchnowski, Jakub","last_name":"Czuchnowski"},{"last_name":"Siggel","full_name":"Siggel, Marc","first_name":"Marc"},{"last_name":"Roessner","first_name":"Rita","full_name":"Roessner, Rita"},{"last_name":"Tschanz","first_name":"Aline","full_name":"Tschanz, Aline"},{"last_name":"Ejsing","full_name":"Ejsing, Christer","first_name":"Christer"},{"last_name":"Schwab","full_name":"Schwab, Yannick","first_name":"Yannick"},{"first_name":"Jan","full_name":"Kosinski, Jan","last_name":"Kosinski"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K"},{"full_name":"Kreshuk, Anna","first_name":"Anna","last_name":"Kreshuk"},{"last_name":"Erzberger","full_name":"Erzberger, Anna","first_name":"Anna"},{"last_name":"Diz-Muñoz","full_name":"Diz-Muñoz, Alba","first_name":"Alba"}],"type":"journal_article","file_date_updated":"2023-09-25T08:22:58Z","quality_controlled":"1","has_accepted_license":"1","status":"public","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"14697"}]},"language":[{"iso":"eng"}],"ddc":["570"],"isi":1,"file":[{"file_size":2725421,"access_level":"open_access","file_id":"14365","content_type":"application/pdf","checksum":"ad670e3b3c64fc585675948370f6b149","creator":"dernst","file_name":"2023_NatureComm_Sitarska.pdf","success":1,"date_created":"2023-09-25T08:22:58Z","date_updated":"2023-09-25T08:22:58Z","relation":"main_file"}],"date_created":"2023-09-24T22:01:10Z","date_updated":"2026-11-14T23:30:13Z"},{"project":[{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients","grant_number":"724373","call_identifier":"H2020"},{"grant_number":"851288","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis"},{"_id":"265E2996-B435-11E9-9278-68D0E5697425","name":"Nano-Analytics of Cellular Systems","grant_number":"W01250-B20","call_identifier":"FWF"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","call_identifier":"H2020"}],"volume":8,"abstract":[{"lang":"eng","text":"Immune responses rely on the rapid and coordinated migration of leukocytes. Whereas it is well established that single-cell migration is often guided by gradients of chemokines and other chemoattractants, it remains poorly understood how these gradients are generated, maintained, and modulated. By combining experimental data with theory on leukocyte chemotaxis guided by the G protein–coupled receptor (GPCR) CCR7, we demonstrate that in addition to its role as the sensory receptor that steers migration, CCR7 also acts as a generator and a modulator of chemotactic gradients. Upon exposure to the CCR7 ligand CCL19, dendritic cells (DCs) effectively internalize the receptor and ligand as part of the canonical GPCR desensitization response. We show that CCR7 internalization also acts as an effective sink for the chemoattractant, dynamically shaping the spatiotemporal distribution of the chemokine. This mechanism drives complex collective migration patterns, enabling DCs to create or sharpen chemotactic gradients. We further show that these self-generated gradients can sustain the long-range guidance of DCs, adapt collective migration patterns to the size and geometry of the environment, and provide a guidance cue for other comigrating cells. Such a dual role of CCR7 as a GPCR that both senses and consumes its ligand can thus provide a novel mode of cellular self-organization."}],"ec_funded":1,"day":"01","acknowledgement":"We thank I. de Vries and the Scientific Service Units (Life Sciences, Bioimaging, Nanofabrication, Preclinical and Miba Machine Shop) of the Institute of Science and Technology Austria for excellent support, as well as all the rotation students assisting in the laboratory work (B. Zens, H. Schön, and D. Babic).\r\nThis work was supported by grants from the European Research Council under the European Union’s Horizon 2020 research to M.S. (grant agreement no. 724373) and to E.H. (grant agreement no. 851288), and a grant by the Austrian Science Fund (DK Nanocell W1250-B20) to M.S. J.A. was supported by the Jenny and Antti Wihuri Foundation and Research Council of Finland's Flagship Programme InFLAMES (decision number: 357910). M.C.U. was supported by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 754411.","fulldoi":"https://doi.org/10.1126/sciimmunol.adc9584","keyword":["General Medicine","Immunology"],"doi":"10.1126/sciimmunol.adc9584","year":"2023","publisher":"American Association for the Advancement of Science","publication":"Science Immunology","external_id":{"pmid":["37656776"],"isi":["001062110600003"]},"pmid":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1126/sciimmunol.adc9584"}],"_id":"14274","publication_identifier":{"issn":["2470-9468"]},"corr_author":"1","oa":1,"department":[{"_id":"MiSi"},{"_id":"EdHa"},{"_id":"NanoFab"}],"article_type":"original","date_published":"2023-09-01T00:00:00Z","intvolume":"         8","article_number":"adc9584","title":"CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration","scopus_import":"1","related_material":{"record":[{"status":"public","id":"14279","relation":"research_data"},{"status":"public","relation":"dissertation_contains","id":"14697"},{"id":"19745","relation":"dissertation_contains","status":"public"}]},"ddc":["570"],"language":[{"iso":"eng"}],"issue":"87","isi":1,"status":"public","date_updated":"2026-11-14T23:30:29Z","date_created":"2023-09-06T08:07:51Z","citation":{"apa":"Alanko, J. H., Ucar, M. C., Canigova, N., Stopp, J. A., Schwarz, J., Merrin, J., … Sixt, M. K. (2023). CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration. <i>Science Immunology</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciimmunol.adc9584\">https://doi.org/10.1126/sciimmunol.adc9584</a>","ieee":"J. H. Alanko <i>et al.</i>, “CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration,” <i>Science Immunology</i>, vol. 8, no. 87. American Association for the Advancement of Science, 2023.","ama":"Alanko JH, Ucar MC, Canigova N, et al. CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration. <i>Science Immunology</i>. 2023;8(87). doi:<a href=\"https://doi.org/10.1126/sciimmunol.adc9584\">10.1126/sciimmunol.adc9584</a>","chicago":"Alanko, Jonna H, Mehmet C Ucar, Nikola Canigova, Julian A Stopp, Jan Schwarz, Jack Merrin, Edouard B Hannezo, and Michael K Sixt. “CCR7 Acts as Both a Sensor and a Sink for CCL19 to Coordinate Collective Leukocyte Migration.” <i>Science Immunology</i>. American Association for the Advancement of Science, 2023. <a href=\"https://doi.org/10.1126/sciimmunol.adc9584\">https://doi.org/10.1126/sciimmunol.adc9584</a>.","mla":"Alanko, Jonna H., et al. “CCR7 Acts as Both a Sensor and a Sink for CCL19 to Coordinate Collective Leukocyte Migration.” <i>Science Immunology</i>, vol. 8, no. 87, adc9584, American Association for the Advancement of Science, 2023, doi:<a href=\"https://doi.org/10.1126/sciimmunol.adc9584\">10.1126/sciimmunol.adc9584</a>.","short":"J.H. Alanko, M.C. Ucar, N. Canigova, J.A. Stopp, J. Schwarz, J. Merrin, E.B. Hannezo, M.K. Sixt, Science Immunology 8 (2023).","ista":"Alanko JH, Ucar MC, Canigova N, Stopp JA, Schwarz J, Merrin J, Hannezo EB, Sixt MK. 2023. CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration. Science Immunology. 8(87), adc9584."},"oa_version":"Published Version","month":"09","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","quality_controlled":"1","publication_status":"published","type":"journal_article","author":[{"first_name":"Jonna H","full_name":"Alanko, Jonna H","orcid":"0000-0002-7698-3061","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","last_name":"Alanko"},{"last_name":"Ucar","id":"50B2A802-6007-11E9-A42B-EB23E6697425","orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C","first_name":"Mehmet C"},{"last_name":"Canigova","id":"3795523E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8518-5926","full_name":"Canigova, Nikola","first_name":"Nikola"},{"first_name":"Julian A","full_name":"Stopp, Julian A","id":"489E3F00-F248-11E8-B48F-1D18A9856A87","last_name":"Stopp"},{"first_name":"Jan","full_name":"Schwarz, Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","last_name":"Schwarz"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609","first_name":"Jack","full_name":"Merrin, Jack"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561","first_name":"Edouard B","full_name":"Hannezo, Edouard B"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K"}]},{"citation":{"short":"L. Nicolai, R. Kaiser, R. Escaig, M.L. Hoffknecht, A. Anjum, A. Leunig, J. Pircher, A. Ehrlich, M. Lorenz, H. Ishikawa-Ankerhold, W.C. Aird, S. Massberg, F.R. Gärtner, Haematologica 107 (2022) 1669–1680.","ista":"Nicolai L, Kaiser R, Escaig R, Hoffknecht ML, Anjum A, Leunig A, Pircher J, Ehrlich A, Lorenz M, Ishikawa-Ankerhold H, Aird WC, Massberg S, Gärtner FR. 2022. Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. Haematologica. 107(7), 1669–1680.","mla":"Nicolai, Leo, et al. “Single Platelet and Megakaryocyte Morpho-Dynamics Uncovered by Multicolor Reporter Mouse Strains in Vitro and in Vivo.” <i>Haematologica</i>, vol. 107, no. 7, Ferrata Storti Foundation, 2022, pp. 1669–80, doi:<a href=\"https://doi.org/10.3324/haematol.2021.278896\">10.3324/haematol.2021.278896</a>.","chicago":"Nicolai, Leo, Rainer Kaiser, Raphael Escaig, Marie Louise Hoffknecht, Afra Anjum, Alexander Leunig, Joachim Pircher, et al. “Single Platelet and Megakaryocyte Morpho-Dynamics Uncovered by Multicolor Reporter Mouse Strains in Vitro and in Vivo.” <i>Haematologica</i>. Ferrata Storti Foundation, 2022. <a href=\"https://doi.org/10.3324/haematol.2021.278896\">https://doi.org/10.3324/haematol.2021.278896</a>.","ieee":"L. Nicolai <i>et al.</i>, “Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo,” <i>Haematologica</i>, vol. 107, no. 7. Ferrata Storti Foundation, pp. 1669–1680, 2022.","apa":"Nicolai, L., Kaiser, R., Escaig, R., Hoffknecht, M. L., Anjum, A., Leunig, A., … Gärtner, F. R. (2022). Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. <i>Haematologica</i>. Ferrata Storti Foundation. <a href=\"https://doi.org/10.3324/haematol.2021.278896\">https://doi.org/10.3324/haematol.2021.278896</a>","ama":"Nicolai L, Kaiser R, Escaig R, et al. Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. <i>Haematologica</i>. 2022;107(7):1669-1680. doi:<a href=\"https://doi.org/10.3324/haematol.2021.278896\">10.3324/haematol.2021.278896</a>"},"tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)","image":"/images/cc_by_nc.png"},"oa_version":"Published Version","month":"07","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","file_date_updated":"2022-07-18T07:51:55Z","quality_controlled":"1","publication_status":"published","type":"journal_article","author":[{"first_name":"Leo","full_name":"Nicolai, Leo","last_name":"Nicolai"},{"last_name":"Kaiser","full_name":"Kaiser, Rainer","first_name":"Rainer"},{"first_name":"Raphael","full_name":"Escaig, Raphael","last_name":"Escaig"},{"first_name":"Marie Louise","full_name":"Hoffknecht, Marie Louise","last_name":"Hoffknecht"},{"last_name":"Anjum","first_name":"Afra","full_name":"Anjum, Afra"},{"last_name":"Leunig","full_name":"Leunig, Alexander","first_name":"Alexander"},{"last_name":"Pircher","first_name":"Joachim","full_name":"Pircher, Joachim"},{"first_name":"Andreas","full_name":"Ehrlich, Andreas","last_name":"Ehrlich"},{"full_name":"Lorenz, Michael","first_name":"Michael","last_name":"Lorenz"},{"first_name":"Hellen","full_name":"Ishikawa-Ankerhold, Hellen","last_name":"Ishikawa-Ankerhold"},{"full_name":"Aird, William C.","first_name":"William C.","last_name":"Aird"},{"full_name":"Massberg, Steffen","first_name":"Steffen","last_name":"Massberg"},{"full_name":"Gärtner, Florian R","first_name":"Florian R","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723"}],"ddc":["570"],"language":[{"iso":"eng"}],"isi":1,"issue":"7","status":"public","has_accepted_license":"1","date_created":"2022-07-17T22:01:54Z","date_updated":"2025-04-14T07:43:16Z","file":[{"file_size":1722094,"access_level":"open_access","file_id":"11595","checksum":"9b47830945f3c30428fe9cfee2dc4a8a","content_type":"application/pdf","date_updated":"2022-07-18T07:51:55Z","relation":"main_file","date_created":"2022-07-18T07:51:55Z","creator":"dernst","file_name":"2022_Haematologica_Nicolai.pdf","success":1}],"_id":"11588","publication_identifier":{"eissn":["1592-8721"],"issn":["0390-6078"]},"corr_author":"1","department":[{"_id":"MiSi"}],"oa":1,"article_type":"original","date_published":"2022-07-01T00:00:00Z","license":"https://creativecommons.org/licenses/by-nc/4.0/","intvolume":"       107","title":"Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo","scopus_import":"1","project":[{"grant_number":"747687","call_identifier":"H2020","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells"}],"volume":107,"ec_funded":1,"abstract":[{"text":"Visualizing cell behavior and effector function on a single cell level has been crucial for understanding key aspects of mammalian biology. Due to their small size, large number and rapid recruitment into thrombi, there is a lack of data on fate and behavior of individual platelets in thrombosis and hemostasis. Here we report the use of platelet lineage restricted multi-color reporter mouse strains to delineate platelet function on a single cell level. We show that genetic labeling allows for single platelet and megakaryocyte (MK) tracking and morphological analysis in vivo and in vitro, while not affecting lineage functions. Using Cre-driven Confetti expression, we provide insights into temporal gene expression patterns as well as spatial clustering of MK in the bone marrow. In the vasculature, shape analysis of activated platelets recruited to thrombi identifies ubiquitous filopodia formation with no evidence of lamellipodia formation. Single cell tracking in complex thrombi reveals prominent myosin-dependent motility of platelets and highlights thrombus formation as a highly dynamic process amenable to modification and intervention of the acto-myosin cytoskeleton. Platelet function assays combining flow cytrometry, as well as in vivo, ex vivo and in vitro imaging show unaltered platelet functions of multicolor reporter mice compared to wild-type controls. In conclusion, platelet lineage multicolor reporter mice prove useful in furthering our understanding of platelet and MK biology on a single cell level.","lang":"eng"}],"acknowledgement":"This study was supported by the Deutsche Forschungsgemeinschaft (DFG) SFB 914 ( to SM [B02 and Z01]), the DFG SFB 1123 (to SM [B06]), the DFG FOR 2033 (to SM), the German\r\nCenter for Cardiovascular Research (DZHK) (Clinician Scientist Programme), MHA 1.4VD (to SM), Postdoc Start-up Grant, 81X3600213 (to FG), 81X3600222 (to LN), the FP7 program\r\n(project 260309, PRESTIGE [to SM]). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 83344, ERC-2018-ADG “IMMUNOTHROMBOSIS” [to SM] and the Marie Skłodowska Curie Individual Fellowship (EU project 747687, LamelliActin [to FG]). ","day":"01","fulldoi":"https://doi.org/10.3324/haematol.2021.278896","year":"2022","doi":"10.3324/haematol.2021.278896","publisher":"Ferrata Storti Foundation","publication":"Haematologica","external_id":{"isi":["000823746100018"]},"page":"1669-1680"},{"_id":"12085","department":[{"_id":"MiSi"}],"oa":1,"publication_identifier":{"issn":["1476-1122"],"eissn":["1476-4660"]},"date_published":"2022-09-01T00:00:00Z","article_type":"original","scopus_import":"1","title":"Weak catch bonds make strong networks","intvolume":"        21","abstract":[{"text":"Molecular catch bonds are ubiquitous in biology and essential for processes like leucocyte extravasion1 and cellular mechanosensing2. Unlike normal (slip) bonds, catch bonds strengthen under tension. The current paradigm is that this feature provides ‘strength on demand3’, thus enabling cells to increase rigidity under stress1,4,5,6. However, catch bonds are often weaker than slip bonds because they have cryptic binding sites that are usually buried7,8. Here we show that catch bonds render reconstituted cytoskeletal actin networks stronger than slip bonds, even though the individual bonds are weaker. Simulations show that slip bonds remain trapped in stress-free areas, whereas weak binding allows catch bonds to mitigate crack initiation by moving to high-tension areas. This ‘dissociation on demand’ explains how cells combine mechanical strength with the adaptability required for shape change, and is relevant to diseases where catch bonding is compromised7,9, including focal segmental glomerulosclerosis10 caused by the α-actinin-4 mutant studied here. We surmise that catch bonds are the key to create life-like materials.","lang":"eng"}],"volume":21,"year":"2022","doi":"10.1038/s41563-022-01288-0","fulldoi":"https://doi.org/10.1038/s41563-022-01288-0","acknowledgement":"We thank M. van Hecke and C. Alkemade for critical reading of the manuscript. We thank P. R. ten Wolde, K. Storm, W. Ellenbroek, C. Broedersz, D. Brueckner and M. Berger for fruitful discussions. We thank W. Brieher and V. Tang from the University of Illinois for the kind gift of purified α-actinin-4 (WT and the K255E point mutant) and their plasmids; M. Kuit-Vinkenoog and J. den Haan for actin and further purification of α-actinin-4; A. Goutou and I. Isturiz-Petitjean for co-sedimentation measurements and V. Sunderlíková for the design, mutagenesis, cloning and purifying of the α-actinin-4 constructs used in the single-molecule experiments. We gratefully acknowledge financial support from the following sources: research program of the Netherlands Organization for Scientific Research (NWO) (S.J.T., A.R. and M.J.A.); ERC Starting Grant (335672-MINICELL) (G.H.K. and Y.M.). ‘BaSyC—Building a Synthetic Cell’ Gravitation grant (024.003.019) of the Netherlands Ministry of Education, Culture and Science (OCW) and the Netherlands Organisation for Scientific Research (G.H.K. and L.B.); and support from the National Institutes of Health (1R01GM126256) (T.K. and W.J.).","day":"01","pmid":1,"publication":"Nature Materials","external_id":{"pmid":["36008604"],"isi":["000844592000002"]},"page":"1019-1023","publisher":"Springer Nature","main_file_link":[{"url":"https://doi.org/10.1101/2020.07.27.219618","open_access":"1"}],"month":"09","oa_version":"Preprint","citation":{"ama":"Mulla Y, Avellaneda Sarrió M, Roland A, et al. Weak catch bonds make strong networks. <i>Nature Materials</i>. 2022;21(9):1019-1023. doi:<a href=\"https://doi.org/10.1038/s41563-022-01288-0\">10.1038/s41563-022-01288-0</a>","apa":"Mulla, Y., Avellaneda Sarrió, M., Roland, A., Baldauf, L., Jung, W., Kim, T., … Koenderink, G. H. (2022). Weak catch bonds make strong networks. <i>Nature Materials</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41563-022-01288-0\">https://doi.org/10.1038/s41563-022-01288-0</a>","ieee":"Y. Mulla <i>et al.</i>, “Weak catch bonds make strong networks,” <i>Nature Materials</i>, vol. 21, no. 9. Springer Nature, pp. 1019–1023, 2022.","chicago":"Mulla, Yuval, Mario Avellaneda Sarrió, Antoine Roland, Lucia Baldauf, Wonyeong Jung, Taeyoon Kim, Sander J. Tans, and Gijsje H. Koenderink. “Weak Catch Bonds Make Strong Networks.” <i>Nature Materials</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41563-022-01288-0\">https://doi.org/10.1038/s41563-022-01288-0</a>.","mla":"Mulla, Yuval, et al. “Weak Catch Bonds Make Strong Networks.” <i>Nature Materials</i>, vol. 21, no. 9, Springer Nature, 2022, pp. 1019–23, doi:<a href=\"https://doi.org/10.1038/s41563-022-01288-0\">10.1038/s41563-022-01288-0</a>.","ista":"Mulla Y, Avellaneda Sarrió M, Roland A, Baldauf L, Jung W, Kim T, Tans SJ, Koenderink GH. 2022. Weak catch bonds make strong networks. Nature Materials. 21(9), 1019–1023.","short":"Y. Mulla, M. Avellaneda Sarrió, A. Roland, L. Baldauf, W. Jung, T. Kim, S.J. Tans, G.H. Koenderink, Nature Materials 21 (2022) 1019–1023."},"article_processing_charge":"No","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","quality_controlled":"1","author":[{"first_name":"Yuval","full_name":"Mulla, Yuval","last_name":"Mulla"},{"full_name":"Avellaneda Sarrió, Mario","first_name":"Mario","orcid":"0000-0001-6406-524X","last_name":"Avellaneda Sarrió","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E"},{"first_name":"Antoine","full_name":"Roland, Antoine","last_name":"Roland"},{"last_name":"Baldauf","first_name":"Lucia","full_name":"Baldauf, Lucia"},{"full_name":"Jung, Wonyeong","first_name":"Wonyeong","last_name":"Jung"},{"last_name":"Kim","full_name":"Kim, Taeyoon","first_name":"Taeyoon"},{"full_name":"Tans, Sander J.","first_name":"Sander J.","last_name":"Tans"},{"last_name":"Koenderink","first_name":"Gijsje H.","full_name":"Koenderink, Gijsje H."}],"type":"journal_article","publication_status":"published","isi":1,"issue":"9","language":[{"iso":"eng"}],"status":"public","date_created":"2022-09-11T22:01:57Z","date_updated":"2023-08-03T14:08:47Z"},{"publisher":"Elsevier","external_id":{"isi":["000922019600003"],"pmid":["36272416"]},"page":"2285-2299.e7","publication":"Immunity","pmid":1,"acknowledgement":"We thank Coung Kieu and Dominik van den Heuvel for excellent technical assistance. This work was supported by the German Research Foundation (PE2704/2-1, PE2704/3-1 to T.P., SFB 1123-project B06 to S.M., SFB1525 project A07 to D.S, TRR 332 project A7 to C.S., PO 2247/2-1 to A.P., SFB1116-project B11 to A.P. and B12 to M.K.), LMU Munich’s Institutional\r\nStrategy LMUexcellent within the framework of the German Excellence Initiative (No. 806 32 006 to T.P.), and by the German Centre for Cardiovascular Research (DZHK) to T.P. (Postdoc Start-up grant No. 100378833). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 833440 to S.M.). F.G. received funding from the European Union’s\r\nHorizon 2020 research and innovation program under the Marie Sk1odowska-Curie grant agreement no. 747687. A.H. was funded by RTI2018-095497-B-I00 from Ministerio de Ciencia e Innovacio´ n (MICINN), HR17_00527 from Fundacion La Caixa, and Transatlantic Network of Excellence (TNE-18CVD04) from the Leducq Foundation. The CNIC is supported by the MICINN and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (CEX2020-001041-S). A.P. was supported by the Forschungskommission of the Medical Faculty of the Heinrich-Heine-Universität Düsseldorf (No. 18-2019 to A.P.). C.G. was supported by the Helmholtz Alliance ‘Aging and Metabolic Programming, AMPro,’ by the German Federal\r\nMinistry of Education and Research to the German Center for Diabetes Research (DZD), and by the Bavarian State Ministry of Health and Care through the research project DigiMed Bayern.","day":"13","keyword":["Infectious Diseases","Immunology","Immunology and Allergy"],"fulldoi":"https://doi.org/10.1016/j.immuni.2022.10.001","doi":"10.1016/j.immuni.2022.10.001","year":"2022","project":[{"name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"747687"}],"volume":55,"abstract":[{"text":"Intravascular neutrophils and platelets collaborate in maintaining host integrity, but their interaction can also trigger thrombotic complications. We report here that cooperation between neutrophil and platelet lineages extends to the earliest stages of platelet formation by megakaryocytes in the bone marrow. Using intravital microscopy, we show that neutrophils “plucked” intravascular megakaryocyte extensions, termed proplatelets, to control platelet production. Following CXCR4-CXCL12-dependent migration towards perisinusoidal megakaryocytes, plucking neutrophils actively pulled on proplatelets and triggered myosin light chain and extracellular-signal-regulated kinase activation through reactive oxygen species. By these mechanisms, neutrophils accelerate proplatelet growth and facilitate continuous release of platelets in steady state. Following myocardial infarction, plucking neutrophils drove excessive release of young, reticulated platelets and boosted the risk of recurrent ischemia. Ablation of neutrophil plucking normalized thrombopoiesis and reduced recurrent thrombosis after myocardial infarction and thrombus burden in venous thrombosis. We establish neutrophil plucking as a target to reduce thromboischemic events.","lang":"eng"}],"ec_funded":1,"intvolume":"        55","scopus_import":"1","title":"Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease","article_type":"original","date_published":"2022-12-13T00:00:00Z","publication_identifier":{"issn":["1074-7613"]},"oa":1,"department":[{"_id":"MiSi"}],"_id":"12119","file":[{"file_size":5299475,"access_level":"open_access","file_id":"12341","checksum":"073267a9c0ad9f85a650053bc7b23777","content_type":"application/pdf","date_created":"2023-01-23T10:18:48Z","date_updated":"2023-01-23T10:18:48Z","relation":"main_file","creator":"dernst","file_name":"2022_Immunity_Petzold.pdf","success":1}],"date_created":"2023-01-12T11:56:54Z","date_updated":"2025-04-14T07:43:16Z","status":"public","has_accepted_license":"1","ddc":["570"],"language":[{"iso":"eng"}],"issue":"12","isi":1,"publication_status":"published","type":"journal_article","author":[{"last_name":"Petzold","full_name":"Petzold, Tobias","first_name":"Tobias"},{"first_name":"Zhe","full_name":"Zhang, Zhe","last_name":"Zhang"},{"last_name":"Ballesteros","first_name":"Iván","full_name":"Ballesteros, Iván"},{"first_name":"Inas","full_name":"Saleh, Inas","last_name":"Saleh"},{"last_name":"Polzin","first_name":"Amin","full_name":"Polzin, Amin"},{"full_name":"Thienel, Manuela","first_name":"Manuela","last_name":"Thienel"},{"full_name":"Liu, Lulu","first_name":"Lulu","last_name":"Liu"},{"last_name":"Ul Ain","full_name":"Ul Ain, Qurrat","first_name":"Qurrat"},{"full_name":"Ehreiser, Vincent","first_name":"Vincent","last_name":"Ehreiser"},{"full_name":"Weber, Christian","first_name":"Christian","last_name":"Weber"},{"full_name":"Kilani, Badr","first_name":"Badr","last_name":"Kilani"},{"last_name":"Mertsch","first_name":"Pontus","full_name":"Mertsch, Pontus"},{"first_name":"Jeremias","full_name":"Götschke, Jeremias","last_name":"Götschke"},{"last_name":"Cremer","first_name":"Sophie","full_name":"Cremer, Sophie"},{"full_name":"Fu, Wenwen","first_name":"Wenwen","last_name":"Fu"},{"full_name":"Lorenz, Michael","first_name":"Michael","last_name":"Lorenz"},{"full_name":"Ishikawa-Ankerhold, Hellen","first_name":"Hellen","last_name":"Ishikawa-Ankerhold"},{"last_name":"Raatz","first_name":"Elisabeth","full_name":"Raatz, Elisabeth"},{"full_name":"El-Nemr, Shaza","first_name":"Shaza","last_name":"El-Nemr"},{"first_name":"Agnes","full_name":"Görlach, Agnes","last_name":"Görlach"},{"full_name":"Marhuenda, Esther","first_name":"Esther","last_name":"Marhuenda"},{"last_name":"Stark","first_name":"Konstantin","full_name":"Stark, Konstantin"},{"last_name":"Pircher","full_name":"Pircher, Joachim","first_name":"Joachim"},{"last_name":"Stegner","first_name":"David","full_name":"Stegner, David"},{"last_name":"Gieger","first_name":"Christian","full_name":"Gieger, Christian"},{"last_name":"Schmidt-Supprian","full_name":"Schmidt-Supprian, Marc","first_name":"Marc"},{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner","orcid":"0000-0001-6120-3723","first_name":"Florian R","full_name":"Gärtner, Florian R"},{"last_name":"Almendros","first_name":"Isaac","full_name":"Almendros, Isaac"},{"last_name":"Kelm","first_name":"Malte","full_name":"Kelm, Malte"},{"full_name":"Schulz, Christian","first_name":"Christian","last_name":"Schulz"},{"last_name":"Hidalgo","first_name":"Andrés","full_name":"Hidalgo, Andrés"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"}],"file_date_updated":"2023-01-23T10:18:48Z","quality_controlled":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"oa_version":"Published Version","citation":{"chicago":"Petzold, Tobias, Zhe Zhang, Iván Ballesteros, Inas Saleh, Amin Polzin, Manuela Thienel, Lulu Liu, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">https://doi.org/10.1016/j.immuni.2022.10.001</a>.","apa":"Petzold, T., Zhang, Z., Ballesteros, I., Saleh, I., Polzin, A., Thienel, M., … Massberg, S. (2022). Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. <i>Immunity</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">https://doi.org/10.1016/j.immuni.2022.10.001</a>","ieee":"T. Petzold <i>et al.</i>, “Neutrophil ‘plucking’ on megakaryocytes drives platelet production and boosts cardiovascular disease,” <i>Immunity</i>, vol. 55, no. 12. Elsevier, p. 2285–2299.e7, 2022.","ama":"Petzold T, Zhang Z, Ballesteros I, et al. Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. <i>Immunity</i>. 2022;55(12):2285-2299.e7. doi:<a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">10.1016/j.immuni.2022.10.001</a>","ista":"Petzold T, Zhang Z, Ballesteros I, Saleh I, Polzin A, Thienel M, Liu L, Ul Ain Q, Ehreiser V, Weber C, Kilani B, Mertsch P, Götschke J, Cremer S, Fu W, Lorenz M, Ishikawa-Ankerhold H, Raatz E, El-Nemr S, Görlach A, Marhuenda E, Stark K, Pircher J, Stegner D, Gieger C, Schmidt-Supprian M, Gärtner FR, Almendros I, Kelm M, Schulz C, Hidalgo A, Massberg S. 2022. Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. Immunity. 55(12), 2285–2299.e7.","short":"T. Petzold, Z. Zhang, I. Ballesteros, I. Saleh, A. Polzin, M. Thienel, L. Liu, Q. Ul Ain, V. Ehreiser, C. Weber, B. Kilani, P. Mertsch, J. Götschke, S. Cremer, W. Fu, M. Lorenz, H. Ishikawa-Ankerhold, E. Raatz, S. El-Nemr, A. Görlach, E. Marhuenda, K. Stark, J. Pircher, D. Stegner, C. Gieger, M. Schmidt-Supprian, F.R. Gärtner, I. Almendros, M. Kelm, C. Schulz, A. Hidalgo, S. Massberg, Immunity 55 (2022) 2285–2299.e7.","mla":"Petzold, Tobias, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>, vol. 55, no. 12, Elsevier, 2022, p. 2285–2299.e7, doi:<a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">10.1016/j.immuni.2022.10.001</a>."},"month":"12"},{"date_created":"2023-01-12T12:03:14Z","date_updated":"2024-10-09T21:03:33Z","status":"public","isi":1,"issue":"12","language":[{"iso":"eng"}],"type":"journal_article","author":[{"id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","last_name":"Cremer","orcid":"0000-0002-2193-3868","first_name":"Sylvia","full_name":"Cremer, Sylvia"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","first_name":"Michael K"}],"publication_status":"published","quality_controlled":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","month":"12","citation":{"mla":"Cremer, Sylvia, and Michael K. Sixt. “Principles of Disease Defence in Organisms, Superorganisms and Societies.” <i>Nature Reviews Immunology</i>, vol. 22, no. 12, Springer Nature, 2022, pp. 713–14, doi:<a href=\"https://doi.org/10.1038/s41577-022-00797-y\">10.1038/s41577-022-00797-y</a>.","ista":"Cremer S, Sixt MK. 2022. Principles of disease defence in organisms, superorganisms and societies. Nature Reviews Immunology. 22(12), 713–714.","short":"S. Cremer, M.K. Sixt, Nature Reviews Immunology 22 (2022) 713–714.","ieee":"S. Cremer and M. K. Sixt, “Principles of disease defence in organisms, superorganisms and societies,” <i>Nature Reviews Immunology</i>, vol. 22, no. 12. Springer Nature, pp. 713–714, 2022.","apa":"Cremer, S., &#38; Sixt, M. K. (2022). Principles of disease defence in organisms, superorganisms and societies. <i>Nature Reviews Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41577-022-00797-y\">https://doi.org/10.1038/s41577-022-00797-y</a>","ama":"Cremer S, Sixt MK. Principles of disease defence in organisms, superorganisms and societies. <i>Nature Reviews Immunology</i>. 2022;22(12):713-714. doi:<a href=\"https://doi.org/10.1038/s41577-022-00797-y\">10.1038/s41577-022-00797-y</a>","chicago":"Cremer, Sylvia, and Michael K Sixt. “Principles of Disease Defence in Organisms, Superorganisms and Societies.” <i>Nature Reviews Immunology</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41577-022-00797-y\">https://doi.org/10.1038/s41577-022-00797-y</a>."},"oa_version":"None","pmid":1,"publisher":"Springer Nature","external_id":{"pmid":["36284178"],"isi":["000871836300001"]},"publication":"Nature Reviews Immunology","page":"713-714","doi":"10.1038/s41577-022-00797-y","year":"2022","day":"01","keyword":["Energy Engineering and Power Technology","Fuel Technology"],"fulldoi":"https://doi.org/10.1038/s41577-022-00797-y","abstract":[{"lang":"eng","text":"Social distancing is an effective way to prevent the spread of disease in societies, whereas infection elimination is a key element of organismal immunity. Here, we discuss how the study of social insects such as ants — which form a superorganism of unconditionally cooperative individuals and thus represent a level of organization that is intermediate between a classical society of individuals and an organism of cells — can help to determine common principles of disease defence across levels of organization."}],"volume":22,"intvolume":"        22","scopus_import":"1","title":"Principles of disease defence in organisms, superorganisms and societies","article_type":"letter_note","date_published":"2022-12-01T00:00:00Z","department":[{"_id":"SyCr"},{"_id":"MiSi"}],"publication_identifier":{"issn":["1474-1733"],"eissn":["1474-1741"]},"corr_author":"1","_id":"12133"},{"title":"Protein chain collapse modulation and folding stimulation by GroEL-ES","scopus_import":"1","intvolume":"         8","article_number":"eabl6293","date_published":"2022-03-01T00:00:00Z","article_type":"original","publication_identifier":{"issn":["2375-2548"]},"department":[{"_id":"MiSi"}],"oa":1,"_id":"17072","publication":"Science Advances","external_id":{"pmid":["35245117"]},"publisher":"American Association for the Advancement of Science","pmid":1,"fulldoi":"https://doi.org/10.1126/sciadv.abl6293","day":"01","acknowledgement":"We thank A. L. Horwich, K. Chakraborty, and B. Schuler for providing plasmids, and R. van Leeuwen, M. Mayer, J. van Zon, W. Noorduin, and P. R. ten Wolde for comments and critical reading of the manuscript. Work in the group of S.J.T. was supported by the Netherlands Organization for Scientific Research (NWO). Work in the group of H.S.R. was supported by a grant from the NIH (R01GM114405).","doi":"10.1126/sciadv.abl6293","year":"2022","volume":8,"abstract":[{"lang":"eng","text":"The collapse of polypeptides is thought important to protein folding, aggregation, intrinsic disorder, and phase separation. However, whether polypeptide collapse is modulated in cells to control protein states is unclear. Here, using integrated protein manipulation and imaging, we show that the chaperonin GroEL-ES can accelerate the folding of proteins by strengthening their collapse. GroEL induces contractile forces in substrate chains, which draws them into the cavity and triggers a general compaction and discrete folding transitions, even for slow-folding proteins. This collapse enhancement is strongest in the nucleotide-bound states of GroEL and is aided by GroES binding to the cavity rim and by the amphiphilic C-terminal tails at the cavity bottom. Collapse modulation is distinct from other proposed GroEL-ES folding acceleration mechanisms, including steric confinement and misfold unfolding. Given the prevalence of collapse throughout the proteome, we conjecture that collapse modulation is more generally relevant within the protein quality control machinery."}],"publication_status":"published","author":[{"full_name":"Naqvi, Mohsin M.","first_name":"Mohsin M.","last_name":"Naqvi"},{"first_name":"Mario","full_name":"Avellaneda Sarrió, Mario","id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","last_name":"Avellaneda Sarrió","orcid":"0000-0001-6406-524X"},{"last_name":"Roth","first_name":"Andrew","full_name":"Roth, Andrew"},{"first_name":"Eline J.","full_name":"Koers, Eline J.","last_name":"Koers"},{"last_name":"Roland","full_name":"Roland, Antoine","first_name":"Antoine"},{"first_name":"Vanda","full_name":"Sunderlikova, Vanda","last_name":"Sunderlikova"},{"last_name":"Kramer","full_name":"Kramer, Günter","first_name":"Günter"},{"first_name":"Hays S.","full_name":"Rye, Hays S.","last_name":"Rye"},{"last_name":"Tans","full_name":"Tans, Sander J.","first_name":"Sander J."}],"type":"journal_article","file_date_updated":"2024-07-31T12:01:51Z","quality_controlled":"1","article_processing_charge":"Yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"ista":"Naqvi MM, Avellaneda Sarrió M, Roth A, Koers EJ, Roland A, Sunderlikova V, Kramer G, Rye HS, Tans SJ. 2022. Protein chain collapse modulation and folding stimulation by GroEL-ES. Science Advances. 8(9), eabl6293.","short":"M.M. Naqvi, M. Avellaneda Sarrió, A. Roth, E.J. Koers, A. Roland, V. Sunderlikova, G. Kramer, H.S. Rye, S.J. Tans, Science Advances 8 (2022).","mla":"Naqvi, Mohsin M., et al. “Protein Chain Collapse Modulation and Folding Stimulation by GroEL-ES.” <i>Science Advances</i>, vol. 8, no. 9, eabl6293, American Association for the Advancement of Science, 2022, doi:<a href=\"https://doi.org/10.1126/sciadv.abl6293\">10.1126/sciadv.abl6293</a>.","chicago":"Naqvi, Mohsin M., Mario Avellaneda Sarrió, Andrew Roth, Eline J. Koers, Antoine Roland, Vanda Sunderlikova, Günter Kramer, Hays S. Rye, and Sander J. Tans. “Protein Chain Collapse Modulation and Folding Stimulation by GroEL-ES.” <i>Science Advances</i>. American Association for the Advancement of Science, 2022. <a href=\"https://doi.org/10.1126/sciadv.abl6293\">https://doi.org/10.1126/sciadv.abl6293</a>.","ama":"Naqvi MM, Avellaneda Sarrió M, Roth A, et al. Protein chain collapse modulation and folding stimulation by GroEL-ES. <i>Science Advances</i>. 2022;8(9). doi:<a href=\"https://doi.org/10.1126/sciadv.abl6293\">10.1126/sciadv.abl6293</a>","ieee":"M. M. Naqvi <i>et al.</i>, “Protein chain collapse modulation and folding stimulation by GroEL-ES,” <i>Science Advances</i>, vol. 8, no. 9. American Association for the Advancement of Science, 2022.","apa":"Naqvi, M. M., Avellaneda Sarrió, M., Roth, A., Koers, E. J., Roland, A., Sunderlikova, V., … Tans, S. J. (2022). Protein chain collapse modulation and folding stimulation by GroEL-ES. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.abl6293\">https://doi.org/10.1126/sciadv.abl6293</a>"},"oa_version":"Published Version","month":"03","file":[{"file_id":"17357","file_size":2404150,"access_level":"open_access","creator":"dernst","file_name":"2022_ScienceAdv_Naqvi.pdf","success":1,"date_updated":"2024-07-31T12:01:51Z","date_created":"2024-07-31T12:01:51Z","relation":"main_file","content_type":"application/pdf","checksum":"9511579306cce7e04107d3d6389ed614"}],"date_updated":"2024-08-05T08:30:29Z","date_created":"2024-05-29T06:12:19Z","has_accepted_license":"1","status":"public","language":[{"iso":"eng"}],"ddc":["570"],"issue":"9"},{"_id":"9794","publication_identifier":{"eissn":["1529-2916"],"issn":["1529-2908"]},"corr_author":"1","department":[{"_id":"SiHi"},{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"MiSi"}],"oa":1,"article_type":"original","date_published":"2022-07-11T00:00:00Z","intvolume":"        23","title":"Multitier mechanics control stromal adaptations in swelling lymph nodes","scopus_import":"1","project":[{"grant_number":"724373","call_identifier":"H2020","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients"}],"volume":23,"abstract":[{"lang":"eng","text":"Lymph nodes (LNs) comprise two main structural elements: fibroblastic reticular cells that form dedicated niches for immune cell interaction and capsular fibroblasts that build a shell around the organ. Immunological challenge causes LNs to increase more than tenfold in size within a few days. Here, we characterized the biomechanics of LN swelling on the cellular and organ scale. We identified lymphocyte trapping by influx and proliferation as drivers of an outward pressure force, causing fibroblastic reticular cells of the T-zone (TRCs) and their associated conduits to stretch. After an initial phase of relaxation, TRCs sensed the resulting strain through cell matrix adhesions, which coordinated local growth and remodeling of the stromal network. While the expanded TRC network readopted its typical configuration, a massive fibrotic reaction of the organ capsule set in and countered further organ expansion. Thus, different fibroblast populations mechanically control LN swelling in a multitier fashion."}],"ec_funded":1,"acknowledgement":"This research was supported by the Scientific Service Units of IST Austria through resources provided by the Imaging and Optics, Electron Microscopy, Preclinical and Life Science Facilities. We thank C. Moussion for providing anti-PNAd antibody and D. Critchley for Talin1-floxed mice, and E. Papusheva for providing a custom 3D channel alignment script. This work was supported by a European Research Council grant ERC-CoG-72437 to M.S. M.H. was supported by Czech Sciencundation GACR 20-24603Y and Charles University PRIMUS/20/MED/013.","day":"11","fulldoi":"https://doi.org/10.1038/s41590-022-01257-4","year":"2022","doi":"10.1038/s41590-022-01257-4","publisher":"Springer Nature","page":"1246-1255","publication":"Nature Immunology","external_id":{"pmid":["35817845"],"isi":["000822975900002"]},"pmid":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","citation":{"mla":"Assen, Frank P., et al. “Multitier Mechanics Control Stromal Adaptations in Swelling Lymph Nodes.” <i>Nature Immunology</i>, vol. 23, Springer Nature, 2022, pp. 1246–55, doi:<a href=\"https://doi.org/10.1038/s41590-022-01257-4\">10.1038/s41590-022-01257-4</a>.","short":"F.P. Assen, J. Abe, M. Hons, R. Hauschild, S. Shamipour, W. Kaufmann, T. Costanzo, G. Krens, M. Brown, B. Ludewig, S. Hippenmeyer, C.-P.J. Heisenberg, W. Weninger, E.B. Hannezo, S.A. Luther, J.V. Stein, M.K. Sixt, Nature Immunology 23 (2022) 1246–1255.","ista":"Assen FP, Abe J, Hons M, Hauschild R, Shamipour S, Kaufmann W, Costanzo T, Krens G, Brown M, Ludewig B, Hippenmeyer S, Heisenberg C-PJ, Weninger W, Hannezo EB, Luther SA, Stein JV, Sixt MK. 2022. Multitier mechanics control stromal adaptations in swelling lymph nodes. Nature Immunology. 23, 1246–1255.","ama":"Assen FP, Abe J, Hons M, et al. Multitier mechanics control stromal adaptations in swelling lymph nodes. <i>Nature Immunology</i>. 2022;23:1246-1255. doi:<a href=\"https://doi.org/10.1038/s41590-022-01257-4\">10.1038/s41590-022-01257-4</a>","ieee":"F. P. Assen <i>et al.</i>, “Multitier mechanics control stromal adaptations in swelling lymph nodes,” <i>Nature Immunology</i>, vol. 23. Springer Nature, pp. 1246–1255, 2022.","apa":"Assen, F. P., Abe, J., Hons, M., Hauschild, R., Shamipour, S., Kaufmann, W., … Sixt, M. K. (2022). Multitier mechanics control stromal adaptations in swelling lymph nodes. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-022-01257-4\">https://doi.org/10.1038/s41590-022-01257-4</a>","chicago":"Assen, Frank P, Jun Abe, Miroslav Hons, Robert Hauschild, Shayan Shamipour, Walter Kaufmann, Tommaso Costanzo, et al. “Multitier Mechanics Control Stromal Adaptations in Swelling Lymph Nodes.” <i>Nature Immunology</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41590-022-01257-4\">https://doi.org/10.1038/s41590-022-01257-4</a>."},"month":"07","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","file_date_updated":"2022-07-25T07:11:32Z","quality_controlled":"1","publication_status":"published","type":"journal_article","author":[{"orcid":"0000-0003-3470-6119","id":"3A8E7F24-F248-11E8-B48F-1D18A9856A87","last_name":"Assen","first_name":"Frank P","full_name":"Assen, Frank P"},{"first_name":"Jun","full_name":"Abe, Jun","last_name":"Abe"},{"last_name":"Hons","id":"4167FE56-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6625-3348","full_name":"Hons, Miroslav","first_name":"Miroslav"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","first_name":"Robert"},{"last_name":"Shamipour","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan","first_name":"Shayan"},{"last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","first_name":"Walter"},{"last_name":"Costanzo","id":"D93824F4-D9BA-11E9-BB12-F207E6697425","orcid":"0000-0001-9732-3815","full_name":"Costanzo, Tommaso","first_name":"Tommaso"},{"first_name":"Gabriel","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","orcid":"0000-0003-4761-5996"},{"last_name":"Brown","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","full_name":"Brown, Markus","first_name":"Markus"},{"last_name":"Ludewig","first_name":"Burkhard","full_name":"Ludewig, Burkhard"},{"first_name":"Simon","full_name":"Hippenmeyer, Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"},{"full_name":"Weninger, Wolfgang","first_name":"Wolfgang","last_name":"Weninger"},{"first_name":"Edouard B","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561"},{"first_name":"Sanjiv A.","full_name":"Luther, Sanjiv A.","last_name":"Luther"},{"first_name":"Jens V.","full_name":"Stein, Jens V.","last_name":"Stein"},{"first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-4561-241X","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt"}],"language":[{"iso":"eng"}],"ddc":["570"],"isi":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"PreCl"},{"_id":"LifeSc"}],"status":"public","has_accepted_license":"1","date_created":"2021-08-06T09:09:11Z","date_updated":"2025-06-11T13:52:43Z","file":[{"file_size":11475325,"access_level":"open_access","file_id":"11642","content_type":"application/pdf","checksum":"628e7b49809f22c75b428842efe70c68","file_name":"2022_NatureImmunology_Assen.pdf","creator":"dernst","success":1,"relation":"main_file","date_updated":"2022-07-25T07:11:32Z","date_created":"2022-07-25T07:11:32Z"}]}]