[{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"Yes","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"ista":"Tomasek K, Leithner AF, Glatzová I, Lukesch MS, Guet CC, Sixt MK. 2022. Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14. eLife. 11, e78995.","short":"K. Tomasek, A.F. Leithner, I. Glatzová, M.S. Lukesch, C.C. Guet, M.K. Sixt, ELife 11 (2022).","mla":"Tomasek, Kathrin, et al. “Type 1 Piliated Uropathogenic Escherichia Coli Hijack the Host Immune Response by Binding to CD14.” <i>ELife</i>, vol. 11, e78995, eLife Sciences Publications, 2022, doi:<a href=\"https://doi.org/10.7554/eLife.78995\">10.7554/eLife.78995</a>.","chicago":"Tomasek, Kathrin, Alexander F Leithner, Ivana Glatzová, Michael S. Lukesch, Calin C Guet, and Michael K Sixt. “Type 1 Piliated Uropathogenic Escherichia Coli Hijack the Host Immune Response by Binding to CD14.” <i>ELife</i>. eLife Sciences Publications, 2022. <a href=\"https://doi.org/10.7554/eLife.78995\">https://doi.org/10.7554/eLife.78995</a>.","ieee":"K. Tomasek, A. F. Leithner, I. Glatzová, M. S. Lukesch, C. C. Guet, and M. K. Sixt, “Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14,” <i>eLife</i>, vol. 11. eLife Sciences Publications, 2022.","apa":"Tomasek, K., Leithner, A. F., Glatzová, I., Lukesch, M. S., Guet, C. C., &#38; Sixt, M. K. (2022). Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.78995\">https://doi.org/10.7554/eLife.78995</a>","ama":"Tomasek K, Leithner AF, Glatzová I, Lukesch MS, Guet CC, Sixt MK. Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14. <i>eLife</i>. 2022;11. doi:<a href=\"https://doi.org/10.7554/eLife.78995\">10.7554/eLife.78995</a>"},"month":"07","publication_status":"published","type":"journal_article","author":[{"first_name":"Kathrin","full_name":"Tomasek, Kathrin","id":"3AEC8556-F248-11E8-B48F-1D18A9856A87","last_name":"Tomasek","orcid":"0000-0003-3768-877X"},{"orcid":"0000-0002-1073-744X","last_name":"Leithner","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","full_name":"Leithner, Alexander F","first_name":"Alexander F"},{"last_name":"Glatzová","id":"727b3c7d-4939-11ec-89b3-b9b0750ab74d","full_name":"Glatzová, Ivana","first_name":"Ivana"},{"first_name":"Michael S.","full_name":"Lukesch, Michael S.","last_name":"Lukesch"},{"last_name":"Guet","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6220-2052","full_name":"Guet, Calin C","first_name":"Calin C"},{"first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"file_date_updated":"2022-08-16T08:57:37Z","quality_controlled":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"EM-Fac"}],"status":"public","has_accepted_license":"1","related_material":{"record":[{"id":"10316","relation":"earlier_version","status":"public"}]},"language":[{"iso":"eng"}],"ddc":["570"],"isi":1,"file":[{"relation":"main_file","date_created":"2022-08-16T08:57:37Z","date_updated":"2022-08-16T08:57:37Z","creator":"cchlebak","file_name":"2022_eLife_Tomasek.pdf","success":1,"checksum":"002a3c7c7ea5caa9af9cfbea308f6ea4","content_type":"application/pdf","file_id":"11861","file_size":2057577,"access_level":"open_access"}],"date_updated":"2026-07-06T12:48:19Z","date_created":"2022-08-14T22:01:46Z","publication_identifier":{"eissn":["2050-084X"]},"corr_author":"1","oa":1,"department":[{"_id":"MiSi"},{"_id":"CaGu"}],"_id":"11843","license":"https://creativecommons.org/licenses/by/4.0/","article_number":"e78995","intvolume":"        11","scopus_import":"1","title":"Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14","article_type":"original","date_published":"2022-07-26T00:00:00Z","acknowledgement":"We thank Ulrich Dobrindt for providing UPEC strains CFT073, UTI89, and 536, Frank Assen, Vlad Gavra, Maximilian Götz, Bor Kavčič, Jonna Alanko, and Eva Kiermaier for help with experiments and Robert Hauschild, Julian Stopp, and Saren Tasciyan for help with data analysis. We thank the IST Austria Scientific Service Units, especially the Bioimaging facility, the Preclinical facility and the Electron microscopy facility for technical support, Jakob Wallner and all members of the Guet and Sixt lab for fruitful discussions and Daria Siekhaus for critically reading the manuscript. This work was supported by grants from the Austrian Research Promotion Agency (FEMtech 868984) to IG, the European Research Council (CoG 724373), and the Austrian Science Fund (FWF P29911) to MS.","day":"26","fulldoi":"https://doi.org/10.7554/eLife.78995","year":"2022","doi":"10.7554/eLife.78995","project":[{"name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"724373"},{"call_identifier":"FWF","grant_number":"P29911","name":"Mechanical adaptation of lamellipodial actin","_id":"26018E70-B435-11E9-9278-68D0E5697425"}],"volume":11,"abstract":[{"text":"A key attribute of persistent or recurring bacterial infections is the ability of the pathogen to evade the host’s immune response. Many Enterobacteriaceae express type 1 pili, a pre-adapted virulence trait, to invade host epithelial cells and establish persistent infections. However, the molecular mechanisms and strategies by which bacteria actively circumvent the immune response of the host remain poorly understood. Here, we identified CD14, the major co-receptor for lipopolysaccharide detection, on mouse dendritic cells (DCs) as a binding partner of FimH, the protein located at the tip of the type 1 pilus of Escherichia coli. The FimH amino acids involved in CD14 binding are highly conserved across pathogenic and non-pathogenic strains. Binding of the pathogenic strain CFT073 to CD14 reduced DC migration by overactivation of integrins and blunted expression of co-stimulatory molecules by overactivating the NFAT (nuclear factor of activated T-cells) pathway, both rate-limiting factors of T cell activation. This response was binary at the single-cell level, but averaged in larger populations exposed to both piliated and non-piliated pathogens, presumably via the exchange of immunomodulatory cytokines. While defining an active molecular mechanism of immune evasion by pathogens, the interaction between FimH and CD14 represents a potential target to interfere with persistent and recurrent infections, such as urinary tract infections or Crohn’s disease.","lang":"eng"}],"ec_funded":1,"publisher":"eLife Sciences Publications","external_id":{"isi":["000838410200001"],"pmid":["35881547"]},"publication":"eLife","pmid":1},{"_id":"10703","corr_author":"1","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"oa":1,"department":[{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"},{"_id":"BjHo"}],"date_published":"2022-01-10T00:00:00Z","article_type":"original","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","title":"WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues","scopus_import":"1","intvolume":"        57","volume":57,"project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","grant_number":"747687","call_identifier":"H2020"},{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients","grant_number":"724373","call_identifier":"H2020"}],"abstract":[{"text":"When crawling through the body, leukocytes often traverse tissues that are densely packed with extracellular matrix and other cells, and this raises the question: How do leukocytes overcome compressive mechanical loads? Here, we show that the actin cortex of leukocytes is mechanoresponsive and that this responsiveness requires neither force sensing via the nucleus nor adhesive interactions with a substrate. Upon global compression of the cell body as well as local indentation of the plasma membrane, Wiskott-Aldrich syndrome protein (WASp) assembles into dot-like structures, providing activation platforms for Arp2/3 nucleated actin patches. These patches locally push against the external load, which can be obstructing collagen fibers or other cells, and thereby create space to facilitate forward locomotion. We show in vitro and in vivo that this WASp function is rate limiting for ameboid leukocyte migration in dense but not in loose environments and is required for trafficking through diverse tissues such as skin and lymph nodes.","lang":"eng"}],"ec_funded":1,"fulldoi":"https://doi.org/10.1016/j.devcel.2021.11.024","day":"10","acknowledgement":"We thank N. Darwish-Miranda, F. Leite, F.P. Assen, and A. Eichner for advice and help with experiments. We thank J. Renkawitz, E. Kiermaier, A. Juanes Garcia, and M. Avellaneda for critical reading of the manuscript. We thank M. Driscoll for advice on fluorescent labeling of collagen gels. This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by Molecular Biology Services/Lab Support Facility (LSF)/Bioimaging Facility/Electron Microscopy Facility. This work was funded by grants from the European Research Council ( CoG 724373 ) and the Austrian Science Foundation (FWF) to M.S. F.G. received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 747687.","year":"2022","doi":"10.1016/j.devcel.2021.11.024","publication":"Developmental Cell","external_id":{"isi":["000768933800005"],"pmid":["34919802"]},"page":"47-62.e9","publisher":"Cell Press","pmid":1,"main_file_link":[{"open_access":"1","url":"https://www.sciencedirect.com/science/article/pii/S1534580721009497"}],"citation":{"chicago":"Gaertner, Florian, Patricia Dos Reis Rodrigues, Ingrid de Vries, Miroslav Hons, Juan Aguilera, Michael Riedl, Alexander F Leithner, et al. “WASp Triggers Mechanosensitive Actin Patches to Facilitate Immune Cell Migration in Dense Tissues.” <i>Developmental Cell</i>. Cell Press, 2022. <a href=\"https://doi.org/10.1016/j.devcel.2021.11.024\">https://doi.org/10.1016/j.devcel.2021.11.024</a>.","ama":"Gaertner F, Dos Reis Rodrigues P, de Vries I, et al. WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. <i>Developmental Cell</i>. 2022;57(1):47-62.e9. doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.11.024\">10.1016/j.devcel.2021.11.024</a>","apa":"Gaertner, F., Dos Reis Rodrigues, P., de Vries, I., Hons, M., Aguilera, J., Riedl, M., … Sixt, M. K. (2022). WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2021.11.024\">https://doi.org/10.1016/j.devcel.2021.11.024</a>","ieee":"F. Gaertner <i>et al.</i>, “WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues,” <i>Developmental Cell</i>, vol. 57, no. 1. Cell Press, p. 47–62.e9, 2022.","short":"F. Gaertner, P. Dos Reis Rodrigues, I. de Vries, M. Hons, J. Aguilera, M. Riedl, A.F. Leithner, S. Tasciyan, A. Kopf, J. Merrin, V. Zheden, W. Kaufmann, R. Hauschild, M.K. Sixt, Developmental Cell 57 (2022) 47–62.e9.","ista":"Gaertner F, Dos Reis Rodrigues P, de Vries I, Hons M, Aguilera J, Riedl M, Leithner AF, Tasciyan S, Kopf A, Merrin J, Zheden V, Kaufmann W, Hauschild R, Sixt MK. 2022. WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. Developmental Cell. 57(1), 47–62.e9.","mla":"Gaertner, Florian, et al. “WASp Triggers Mechanosensitive Actin Patches to Facilitate Immune Cell Migration in Dense Tissues.” <i>Developmental Cell</i>, vol. 57, no. 1, Cell Press, 2022, p. 47–62.e9, doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.11.024\">10.1016/j.devcel.2021.11.024</a>."},"tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"oa_version":"Published Version","month":"01","article_processing_charge":"No","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","quality_controlled":"1","publication_status":"published","author":[{"last_name":"Gaertner","full_name":"Gaertner, Florian","first_name":"Florian"},{"orcid":"0000-0003-1681-508X","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","last_name":"Dos Reis Rodrigues","first_name":"Patricia","full_name":"Dos Reis Rodrigues, Patricia"},{"first_name":"Ingrid","full_name":"De Vries, Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","last_name":"De Vries"},{"orcid":"0000-0002-6625-3348","last_name":"Hons","id":"4167FE56-F248-11E8-B48F-1D18A9856A87","full_name":"Hons, Miroslav","first_name":"Miroslav"},{"last_name":"Aguilera","full_name":"Aguilera, Juan","first_name":"Juan"},{"last_name":"Riedl","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4844-6311","full_name":"Riedl, Michael","first_name":"Michael"},{"orcid":"0000-0002-1073-744X","last_name":"Leithner","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","full_name":"Leithner, Alexander F","first_name":"Alexander F"},{"first_name":"Saren","full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","last_name":"Tasciyan"},{"first_name":"Aglaja","full_name":"Kopf, Aglaja","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","last_name":"Kopf","orcid":"0000-0002-2187-6656"},{"first_name":"Jack","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin"},{"orcid":"0000-0002-9438-4783","last_name":"Zheden","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","full_name":"Zheden, Vanessa","first_name":"Vanessa"},{"last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","first_name":"Walter"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K"}],"type":"journal_article","language":[{"iso":"eng"}],"ddc":["570"],"related_material":{"record":[{"status":"public","id":"20149","relation":"dissertation_contains"},{"id":"12726","relation":"dissertation_contains","status":"public"},{"id":"14530","relation":"dissertation_contains","status":"public"},{"status":"public","relation":"dissertation_contains","id":"12401"}]},"issue":"1","isi":1,"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"EM-Fac"}],"status":"public","date_created":"2022-01-30T23:01:33Z","date_updated":"2026-11-14T23:30:12Z"},{"author":[{"last_name":"Tasciyan","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren","first_name":"Saren"}],"type":"dissertation","publication_status":"published","alternative_title":["ISTA Thesis"],"supervisor":[{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"}],"file_date_updated":"2023-12-21T23:30:03Z","article_processing_charge":"No","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","month":"12","oa_version":"Published Version","citation":{"ieee":"S. Tasciyan, “Role of microenvironment heterogeneity in cancer cell invasion,” Institute of Science and Technology Austria, 2022.","apa":"Tasciyan, S. (2022). <i>Role of microenvironment heterogeneity in cancer cell invasion</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:12401\">https://doi.org/10.15479/at:ista:12401</a>","ama":"Tasciyan S. Role of microenvironment heterogeneity in cancer cell invasion. 2022. doi:<a href=\"https://doi.org/10.15479/at:ista:12401\">10.15479/at:ista:12401</a>","chicago":"Tasciyan, Saren. “Role of Microenvironment Heterogeneity in Cancer Cell Invasion.” Institute of Science and Technology Austria, 2022. <a href=\"https://doi.org/10.15479/at:ista:12401\">https://doi.org/10.15479/at:ista:12401</a>.","mla":"Tasciyan, Saren. <i>Role of Microenvironment Heterogeneity in Cancer Cell Invasion</i>. Institute of Science and Technology Austria, 2022, doi:<a href=\"https://doi.org/10.15479/at:ista:12401\">10.15479/at:ista:12401</a>.","short":"S. Tasciyan, Role of Microenvironment Heterogeneity in Cancer Cell Invasion, Institute of Science and Technology Austria, 2022.","ista":"Tasciyan S. 2022. Role of microenvironment heterogeneity in cancer cell invasion. Institute of Science and Technology Austria."},"file":[{"access_level":"open_access","file_size":42059787,"file_id":"12402","embargo":"2023-12-20","content_type":"application/pdf","checksum":"cc4a2b4a7e3c4ee8ef7f2dbf909b12bd","creator":"cchlebak","file_name":"PhD-Thesis_Saren Tasciyan_formatted_aftercrash_fixed_600dpi_95pc_final_PDFA3b.pdf","date_created":"2023-01-26T11:58:14Z","relation":"main_file","date_updated":"2023-12-21T23:30:03Z"},{"access_level":"closed","embargo_to":"open_access","file_size":261256696,"file_id":"12403","checksum":"f1b4ca98b8ab0cb043b1830971e9bd9c","content_type":"application/x-zip-compressed","date_created":"2023-01-26T12:00:10Z","relation":"source_file","date_updated":"2023-12-21T23:30:03Z","file_name":"Source Files - Saren Tasciyan - PhD Thesis.zip","creator":"cchlebak"}],"date_created":"2023-01-26T11:55:16Z","date_updated":"2026-04-14T09:07:14Z","has_accepted_license":"1","status":"public","degree_awarded":"PhD","language":[{"iso":"eng"}],"ddc":["610"],"related_material":{"record":[{"id":"7885","relation":"part_of_dissertation","status":"public"},{"relation":"part_of_dissertation","id":"10703","status":"public"},{"relation":"part_of_dissertation","id":"679","status":"public"},{"status":"public","id":"9429","relation":"part_of_dissertation"}]},"OA_place":"publisher","title":"Role of microenvironment heterogeneity in cancer cell invasion","date_published":"2022-12-22T00:00:00Z","oa":1,"department":[{"_id":"GradSch"},{"_id":"MiSi"}],"corr_author":"1","publication_identifier":{"issn":["2663-337X"]},"_id":"12401","page":"105","publisher":"Institute of Science and Technology Austria","doi":"10.15479/at:ista:12401","year":"2022","fulldoi":"https://doi.org/10.15479/at:ista:12401","day":"22","abstract":[{"text":"Detachment of the cancer cells from the bulk of the tumor is the first step of metastasis, which\r\nis the primary cause of cancer related deaths. It is unclear, which factors contribute to this step.\r\nRecent studies indicate a crucial role of the tumor microenvironment in malignant\r\ntransformation and metastasis. Studying cancer cell invasion and detachments quantitatively in\r\nthe context of its physiological microenvironment is technically challenging. Especially, precise\r\ncontrol of microenvironmental properties in vivo is currently not possible. Here, I studied the\r\nrole of microenvironment geometry in the invasion and detachment of cancer cells from the\r\nbulk with a simplistic and reductionist approach. In this approach, I engineered microfluidic\r\ndevices to mimic a pseudo 3D extracellular matrix environment, where I was able to\r\nquantitatively tune the geometrical configuration of the microenvironment and follow tumor\r\ncells with fluorescence live imaging. To aid quantitative analysis I developed a widely applicable\r\nsoftware application to automatically analyze and visualize particle tracking data.\r\nQuantitative analysis of tumor cell invasion in isotropic and anisotropic microenvironments\r\nshowed that heterogeneity in the microenvironment promotes faster invasion and more\r\nfrequent detachment of cells. These observations correlated with overall higher speed of cells at\r\nthe edge of the bulk of the cells. In heterogeneous microenvironments cells preferentially\r\npassed through larger pores, thus invading areas of least resistance and generating finger-like\r\ninvasive structures. The detachments occurred mostly at the tips of these structures.\r\nTo investigate the potential mechanism, we established a two dimensional model to simulate\r\nactive Brownian particles representing the cell nuclei dynamics. These simulations backed our in\r\nvitro observations without the need of precise fitting the simulation parameters. Our model\r\nsuggests the importance of the pore heterogeneity in the direction perpendicular to the\r\norientation of bias field (lateral heterogeneity), which causes the interface roughening.","lang":"eng"}]},{"license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","scopus_import":"1","title":"Plan your trip before you leave: The neutrophils’ search-and-run journey","article_number":"e202206127","intvolume":"       221","date_published":"2022-07-20T00:00:00Z","article_type":"original","corr_author":"1","publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"oa":1,"department":[{"_id":"MiSi"}],"_id":"12272","external_id":{"isi":["000874717200001"],"pmid":["35856919"]},"publication":"Journal of Cell Biology","publisher":"Rockefeller University Press","pmid":1,"keyword":["Cell Biology"],"fulldoi":"https://doi.org/10.1083/jcb.202206127","day":"20","year":"2022","doi":"10.1083/jcb.202206127","volume":221,"abstract":[{"lang":"eng","text":"Reading, interpreting and crawling along gradients of chemotactic cues is one of the most complex questions in cell biology. In this issue, Georgantzoglou et al. (2022. J. Cell. Biol.https://doi.org/10.1083/jcb.202103207) use in vivo models to map the temporal sequence of how neutrophils respond to an acutely arising gradient of chemoattractant."}],"publication_status":"published","author":[{"last_name":"Stopp","id":"489E3F00-F248-11E8-B48F-1D18A9856A87","full_name":"Stopp, Julian A","first_name":"Julian A"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"}],"type":"journal_article","file_date_updated":"2023-01-30T10:39:34Z","quality_controlled":"1","article_processing_charge":"No","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"chicago":"Stopp, Julian A, and Michael K Sixt. “Plan Your Trip before You Leave: The Neutrophils’ Search-and-Run Journey.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2022. <a href=\"https://doi.org/10.1083/jcb.202206127\">https://doi.org/10.1083/jcb.202206127</a>.","ama":"Stopp JA, Sixt MK. Plan your trip before you leave: The neutrophils’ search-and-run journey. <i>Journal of Cell Biology</i>. 2022;221(8). doi:<a href=\"https://doi.org/10.1083/jcb.202206127\">10.1083/jcb.202206127</a>","apa":"Stopp, J. A., &#38; Sixt, M. K. (2022). Plan your trip before you leave: The neutrophils’ search-and-run journey. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202206127\">https://doi.org/10.1083/jcb.202206127</a>","ieee":"J. A. Stopp and M. K. Sixt, “Plan your trip before you leave: The neutrophils’ search-and-run journey,” <i>Journal of Cell Biology</i>, vol. 221, no. 8. Rockefeller University Press, 2022.","ista":"Stopp JA, Sixt MK. 2022. Plan your trip before you leave: The neutrophils’ search-and-run journey. Journal of Cell Biology. 221(8), e202206127.","short":"J.A. Stopp, M.K. Sixt, Journal of Cell Biology 221 (2022).","mla":"Stopp, Julian A., and Michael K. Sixt. “Plan Your Trip before You Leave: The Neutrophils’ Search-and-Run Journey.” <i>Journal of Cell Biology</i>, vol. 221, no. 8, e202206127, Rockefeller University Press, 2022, doi:<a href=\"https://doi.org/10.1083/jcb.202206127\">10.1083/jcb.202206127</a>."},"tmp":{"name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","image":"/images/cc_by_nc_sa.png","short":"CC BY-NC-SA (4.0)"},"oa_version":"Published Version","month":"07","file":[{"file_id":"12451","access_level":"open_access","file_size":969969,"date_updated":"2023-01-30T10:39:34Z","relation":"main_file","date_created":"2023-01-30T10:39:34Z","success":1,"creator":"dernst","file_name":"2022_JourCellBiology_Stopp.pdf","checksum":"6b1620743669679b48b9389bb40f5a11","content_type":"application/pdf"}],"date_created":"2023-01-16T10:01:08Z","date_updated":"2026-11-14T23:30:13Z","has_accepted_license":"1","status":"public","language":[{"iso":"eng"}],"ddc":["570"],"related_material":{"record":[{"status":"public","id":"14697","relation":"dissertation_contains"}]},"issue":"8","isi":1},{"main_file_link":[{"url":"https://doi.org/10.1101/2020.03.24.005835","open_access":"1"}],"pmid":1,"publisher":"Elsevier","publication":"Current Biology","external_id":{"isi":["000654652200002"],"pmid":["33711252"]},"page":"2051-2064.e8","doi":"10.1016/j.cub.2021.02.043","year":"2021","day":"24","acknowledgement":"We are grateful to Silvia Prettin, Ina Schleicher, and Petra Hagendorff for expert technical assistance; David Dettbarn for animal keeping and breeding; and Lothar Gröbe and Maria Höxter for cell sorting. We also thank Werner Tegge for peptides and Giorgio Scita for antibodies. This work was supported, in part, by the Deutsche Forschungsgemeinschaft (DFG), Priority Programm SPP1150 (to T.E.B.S., K.R., and M. Sixt), and by DFG grant GRK2223/1 (to K.R.). T.E.B.S. acknowledges support by the Helmholtz Society through HGF impulse fund W2/W3-066 and M. Schnoor by the Mexican Council for Science and Technology (CONACyT, 284292 ), Fund SEP-Cinvestav ( 108 ), and the Royal Society, UK (Newton Advanced Fellowship, NAF/R1/180017 ).","fulldoi":"https://doi.org/10.1016/j.cub.2021.02.043","keyword":["General Agricultural and Biological Sciences","General Biochemistry","Genetics and Molecular Biology"],"abstract":[{"text":"Hematopoietic-specific protein 1 (Hem1) is an essential subunit of the WAVE regulatory complex (WRC) in immune cells. WRC is crucial for Arp2/3 complex activation and the protrusion of branched actin filament networks. Moreover, Hem1 loss of function in immune cells causes autoimmune diseases in humans. Here, we show that genetic removal of Hem1 in macrophages diminishes frequency and efficacy of phagocytosis as well as phagocytic cup formation in addition to defects in lamellipodial protrusion and migration. Moreover, Hem1-null macrophages displayed strong defects in cell adhesion despite unaltered podosome formation and concomitant extracellular matrix degradation. Specifically, dynamics of both adhesion and de-adhesion as well as concomitant phosphorylation of paxillin and focal adhesion kinase (FAK) were significantly compromised. Accordingly, disruption of WRC function in non-hematopoietic cells coincided with both defects in adhesion turnover and altered FAK and paxillin phosphorylation. Consistently, platelets exhibited reduced adhesion and diminished integrin αIIbβ3 activation upon WRC removal. Interestingly, adhesion phenotypes, but not lamellipodia formation, were partially rescued by small molecule activation of FAK. A full rescue of the phenotype, including lamellipodia formation, required not only the presence of WRCs but also their binding to and activation by Rac. Collectively, our results uncover that WRC impacts on integrin-dependent processes in a FAK-dependent manner, controlling formation and dismantling of adhesions, relevant for properly grabbing onto extracellular surfaces and particles during cell edge expansion, like in migration or phagocytosis.","lang":"eng"}],"volume":31,"intvolume":"        31","scopus_import":"1","title":"Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion","article_type":"original","date_published":"2021-05-24T00:00:00Z","oa":1,"department":[{"_id":"MiSi"}],"publication_identifier":{"issn":["0960-9822"]},"_id":"10834","date_created":"2022-03-08T07:51:04Z","date_updated":"2023-08-17T07:01:14Z","status":"public","isi":1,"issue":"10","language":[{"iso":"eng"}],"type":"journal_article","author":[{"first_name":"Stephanie","full_name":"Stahnke, Stephanie","last_name":"Stahnke"},{"last_name":"Döring","full_name":"Döring, Hermann","first_name":"Hermann"},{"last_name":"Kusch","full_name":"Kusch, Charly","first_name":"Charly"},{"last_name":"de Gorter","full_name":"de Gorter, David J.J.","first_name":"David J.J."},{"last_name":"Dütting","full_name":"Dütting, Sebastian","first_name":"Sebastian"},{"full_name":"Guledani, Aleks","first_name":"Aleks","last_name":"Guledani"},{"full_name":"Pleines, Irina","first_name":"Irina","last_name":"Pleines"},{"first_name":"Michael","full_name":"Schnoor, Michael","last_name":"Schnoor"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","first_name":"Michael K"},{"last_name":"Geffers","first_name":"Robert","full_name":"Geffers, Robert"},{"first_name":"Manfred","full_name":"Rohde, Manfred","last_name":"Rohde"},{"first_name":"Mathias","full_name":"Müsken, Mathias","last_name":"Müsken"},{"last_name":"Kage","full_name":"Kage, Frieda","first_name":"Frieda"},{"full_name":"Steffen, Anika","first_name":"Anika","last_name":"Steffen"},{"full_name":"Faix, Jan","first_name":"Jan","last_name":"Faix"},{"last_name":"Nieswandt","full_name":"Nieswandt, Bernhard","first_name":"Bernhard"},{"last_name":"Rottner","full_name":"Rottner, Klemens","first_name":"Klemens"},{"last_name":"Stradal","first_name":"Theresia E.B.","full_name":"Stradal, Theresia E.B."}],"publication_status":"published","quality_controlled":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","month":"05","oa_version":"Preprint","citation":{"chicago":"Stahnke, Stephanie, Hermann Döring, Charly Kusch, David J.J. de Gorter, Sebastian Dütting, Aleks Guledani, Irina Pleines, et al. “Loss of Hem1 Disrupts Macrophage Function and Impacts Migration, Phagocytosis, and Integrin-Mediated Adhesion.” <i>Current Biology</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">https://doi.org/10.1016/j.cub.2021.02.043</a>.","ama":"Stahnke S, Döring H, Kusch C, et al. Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. <i>Current Biology</i>. 2021;31(10):2051-2064.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">10.1016/j.cub.2021.02.043</a>","apa":"Stahnke, S., Döring, H., Kusch, C., de Gorter, D. J. J., Dütting, S., Guledani, A., … Stradal, T. E. B. (2021). Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">https://doi.org/10.1016/j.cub.2021.02.043</a>","ieee":"S. Stahnke <i>et al.</i>, “Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion,” <i>Current Biology</i>, vol. 31, no. 10. Elsevier, p. 2051–2064.e8, 2021.","short":"S. Stahnke, H. Döring, C. Kusch, D.J.J. de Gorter, S. Dütting, A. Guledani, I. Pleines, M. Schnoor, M.K. Sixt, R. Geffers, M. Rohde, M. Müsken, F. Kage, A. Steffen, J. Faix, B. Nieswandt, K. Rottner, T.E.B. Stradal, Current Biology 31 (2021) 2051–2064.e8.","ista":"Stahnke S, Döring H, Kusch C, de Gorter DJJ, Dütting S, Guledani A, Pleines I, Schnoor M, Sixt MK, Geffers R, Rohde M, Müsken M, Kage F, Steffen A, Faix J, Nieswandt B, Rottner K, Stradal TEB. 2021. Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. Current Biology. 31(10), 2051–2064.e8.","mla":"Stahnke, Stephanie, et al. “Loss of Hem1 Disrupts Macrophage Function and Impacts Migration, Phagocytosis, and Integrin-Mediated Adhesion.” <i>Current Biology</i>, vol. 31, no. 10, Elsevier, 2021, p. 2051–2064.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">10.1016/j.cub.2021.02.043</a>."}},{"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1073/pnas.2010054118"}],"pmid":1,"publisher":"National Academy of Sciences","publication":"Proceedings of the National Academy of Sciences of the United States of America","external_id":{"isi":["000607270100018"],"pmid":["33443153"]},"doi":"10.1073/pnas.2010054118","year":"2021","acknowledgement":"We thank Urban Bezeljak, Natalia Baranova, Mar Lopez-Pelegrin, Catarina Alcarva, and Victoria Faas for sharing reagents and helpful discussions. We thank Veronika Szentirmai for help with protein purifications. We thank Carrie Bernecky, Sascha Martens, and the M.L. lab for comments on the manuscript. We thank the bioimaging facility, the life science facility, and Armel Nicolas from the mass spec facility at the Institute of Science and Technology (IST) Austria for technical support. C.D. acknowledges funding from the IST fellowship program; this work was supported by Human Frontier Science Program Young Investigator Grant\r\nRGY0083/2016. ","day":"05","fulldoi":"https://doi.org/10.1073/pnas.2010054118","abstract":[{"text":"The differentiation of cells depends on a precise control of their internal organization, which is the result of a complex dynamic interplay between the cytoskeleton, molecular motors, signaling molecules, and membranes. For example, in the developing neuron, the protein ADAP1 (ADP-ribosylation factor GTPase-activating protein [ArfGAP] with dual pleckstrin homology [PH] domains 1) has been suggested to control dendrite branching by regulating the small GTPase ARF6. Together with the motor protein KIF13B, ADAP1 is also thought to mediate delivery of the second messenger phosphatidylinositol (3,4,5)-trisphosphate (PIP3) to the axon tip, thus contributing to PIP3 polarity. However, what defines the function of ADAP1 and how its different roles are coordinated are still not clear. Here, we studied ADAP1’s functions using in vitro reconstitutions. We found that KIF13B transports ADAP1 along microtubules, but that PIP3 as well as PI(3,4)P2 act as stop signals for this transport instead of being transported. We also demonstrate that these phosphoinositides activate ADAP1’s enzymatic activity to catalyze GTP hydrolysis by ARF6. Together, our results support a model for the cellular function of ADAP1, where KIF13B transports ADAP1 until it encounters high PIP3/PI(3,4)P2 concentrations in the plasma membrane. Here, ADAP1 disassociates from the motor to inactivate ARF6, promoting dendrite branching.","lang":"eng"}],"project":[{"name":"Reconstitution of cell polarity and axis determination in a cell-free system","_id":"2599F062-B435-11E9-9278-68D0E5697425","grant_number":"RGY0083/2016"}],"volume":118,"intvolume":"       118","article_number":"e2010054118","title":"In vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1","scopus_import":"1","article_type":"original","date_published":"2021-01-05T00:00:00Z","oa":1,"department":[{"_id":"MaLo"},{"_id":"MiSi"}],"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"corr_author":"1","_id":"8988","date_created":"2021-01-03T23:01:23Z","date_updated":"2026-06-18T19:37:53Z","status":"public","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"isi":1,"issue":"1","ddc":["570"],"language":[{"iso":"eng"}],"type":"journal_article","author":[{"full_name":"Düllberg, Christian F","first_name":"Christian F","orcid":"0000-0001-6335-9748","last_name":"Düllberg","id":"459064DC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Auer, Albert","first_name":"Albert","last_name":"Auer","id":"3018E8C2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3580-2906"},{"full_name":"Canigova, Nikola","first_name":"Nikola","orcid":"0000-0002-8518-5926","last_name":"Canigova","id":"3795523E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Loibl, Katrin","first_name":"Katrin","last_name":"Loibl","id":"3760F32C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2429-7668"},{"full_name":"Loose, Martin","first_name":"Martin","last_name":"Loose","id":"462D4284-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7309-9724"}],"publication_status":"published","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","month":"01","oa_version":"Published Version","citation":{"ista":"Düllberg CF, Auer A, Canigova N, Loibl K, Loose M. 2021. In vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1. Proceedings of the National Academy of Sciences of the United States of America. 118(1), e2010054118.","short":"C.F. Düllberg, A. Auer, N. Canigova, K. Loibl, M. Loose, Proceedings of the National Academy of Sciences of the United States of America 118 (2021).","mla":"Düllberg, Christian F., et al. “In Vitro Reconstitution Reveals Phosphoinositides as Cargo-Release Factors and Activators of the ARF6 GAP ADAP1.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 1, e2010054118, National Academy of Sciences, 2021, doi:<a href=\"https://doi.org/10.1073/pnas.2010054118\">10.1073/pnas.2010054118</a>.","chicago":"Düllberg, Christian F, Albert Auer, Nikola Canigova, Katrin Loibl, and Martin Loose. “In Vitro Reconstitution Reveals Phosphoinositides as Cargo-Release Factors and Activators of the ARF6 GAP ADAP1.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2021. <a href=\"https://doi.org/10.1073/pnas.2010054118\">https://doi.org/10.1073/pnas.2010054118</a>.","ama":"Düllberg CF, Auer A, Canigova N, Loibl K, Loose M. In vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2021;118(1). doi:<a href=\"https://doi.org/10.1073/pnas.2010054118\">10.1073/pnas.2010054118</a>","apa":"Düllberg, C. F., Auer, A., Canigova, N., Loibl, K., &#38; Loose, M. (2021). In vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2010054118\">https://doi.org/10.1073/pnas.2010054118</a>","ieee":"C. F. Düllberg, A. Auer, N. Canigova, K. Loibl, and M. Loose, “In vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 1. National Academy of Sciences, 2021."}},{"file":[{"content_type":"application/pdf","checksum":"843ebc153847c8626e13c9c5ce71d533","success":1,"creator":"dernst","file_name":"2021_JournCellBiology_Leithner.pdf","relation":"main_file","date_created":"2022-05-12T14:16:21Z","date_updated":"2022-05-12T14:16:21Z","access_level":"open_access","file_size":5102328,"file_id":"11367"}],"date_created":"2021-02-05T10:08:04Z","date_updated":"2024-10-09T21:00:23Z","status":"public","has_accepted_license":"1","ddc":["570"],"language":[{"iso":"eng"}],"issue":"4","isi":1,"publication_status":"published","type":"journal_article","author":[{"orcid":"0000-0002-1073-744X","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","last_name":"Leithner","first_name":"Alexander F","full_name":"Leithner, Alexander F"},{"first_name":"LM","full_name":"Altenburger, LM","last_name":"Altenburger"},{"last_name":"Hauschild","full_name":"Hauschild, R","first_name":"R"},{"first_name":"Frank P","full_name":"Assen, Frank P","orcid":"0000-0003-3470-6119","id":"3A8E7F24-F248-11E8-B48F-1D18A9856A87","last_name":"Assen"},{"full_name":"Rottner, K","first_name":"K","last_name":"Rottner"},{"last_name":"TEB","full_name":"TEB, Stradal","first_name":"Stradal"},{"first_name":"A","full_name":"Diz-Muñoz, A","last_name":"Diz-Muñoz"},{"first_name":"JV","full_name":"Stein, JV","last_name":"Stein"},{"full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"file_date_updated":"2022-05-12T14:16:21Z","quality_controlled":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"No","tmp":{"name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","image":"/images/cc_by_nc_sa.png","short":"CC BY-NC-SA (4.0)"},"oa_version":"Published Version","citation":{"chicago":"Leithner, Alexander F, LM Altenburger, R Hauschild, Frank P Assen, K Rottner, Stradal TEB, A Diz-Muñoz, JV Stein, and Michael K Sixt. “Dendritic Cell Actin Dynamics Control Contact Duration and Priming Efficiency at the Immunological Synapse.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2021. <a href=\"https://doi.org/10.1083/jcb.202006081\">https://doi.org/10.1083/jcb.202006081</a>.","ieee":"A. F. Leithner <i>et al.</i>, “Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse,” <i>Journal of Cell Biology</i>, vol. 220, no. 4. Rockefeller University Press, 2021.","apa":"Leithner, A. F., Altenburger, L., Hauschild, R., Assen, F. P., Rottner, K., TEB, S., … Sixt, M. K. (2021). Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202006081\">https://doi.org/10.1083/jcb.202006081</a>","ama":"Leithner AF, Altenburger L, Hauschild R, et al. Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse. <i>Journal of Cell Biology</i>. 2021;220(4). doi:<a href=\"https://doi.org/10.1083/jcb.202006081\">10.1083/jcb.202006081</a>","ista":"Leithner AF, Altenburger L, Hauschild R, Assen FP, Rottner K, TEB S, Diz-Muñoz A, Stein J, Sixt MK. 2021. Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse. Journal of Cell Biology. 220(4), e202006081.","short":"A.F. Leithner, L. Altenburger, R. Hauschild, F.P. Assen, K. Rottner, S. TEB, A. Diz-Muñoz, J. Stein, M.K. Sixt, Journal of Cell Biology 220 (2021).","mla":"Leithner, Alexander F., et al. “Dendritic Cell Actin Dynamics Control Contact Duration and Priming Efficiency at the Immunological Synapse.” <i>Journal of Cell Biology</i>, vol. 220, no. 4, e202006081, Rockefeller University Press, 2021, doi:<a href=\"https://doi.org/10.1083/jcb.202006081\">10.1083/jcb.202006081</a>."},"month":"04","publisher":"Rockefeller University Press","external_id":{"isi":["000626365700001"],"pmid":["33533935"]},"publication":"Journal of Cell Biology","pmid":1,"day":"05","fulldoi":"https://doi.org/10.1083/jcb.202006081","year":"2021","doi":"10.1083/jcb.202006081","volume":220,"abstract":[{"text":"Dendritic cells (DCs) are crucial for the priming of naive T cells and the initiation of adaptive immunity. Priming is initiated at a heterologous cell–cell contact, the immunological synapse (IS). While it is established that F-actin dynamics regulates signaling at the T cell side of the contact, little is known about the cytoskeletal contribution on the DC side. Here, we show that the DC actin cytoskeleton is decisive for the formation of a multifocal synaptic structure, which correlates with T cell priming efficiency. DC actin at the IS appears in transient foci that are dynamized by the WAVE regulatory complex (WRC). The absence of the WRC in DCs leads to stabilized contacts with T cells, caused by an increase in ICAM1-integrin–mediated cell–cell adhesion. This results in lower numbers of activated and proliferating T cells, demonstrating an important role for DC actin in the regulation of immune synapse functionality.","lang":"eng"}],"article_number":"e202006081","intvolume":"       220","scopus_import":"1","title":"Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse","article_type":"original","date_published":"2021-04-05T00:00:00Z","publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"corr_author":"1","department":[{"_id":"MiSi"}],"oa":1,"_id":"9094"},{"date_published":"2021-02-25T00:00:00Z","article_type":"original","title":"Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium","scopus_import":"1","article_number":"630002","intvolume":"        12","_id":"9259","oa":1,"department":[{"_id":"MiSi"},{"_id":"Bio"}],"corr_author":"1","publication_identifier":{"eissn":["1664-3224"]},"pmid":1,"publication":"Frontiers in Immunology","external_id":{"isi":["000627134400001"],"pmid":["33717158"]},"publisher":"Frontiers","ec_funded":1,"abstract":[{"lang":"eng","text":"Gradients of chemokines and growth factors guide migrating cells and morphogenetic processes. Migration of antigen-presenting dendritic cells from the interstitium into the lymphatic system is dependent on chemokine CCL21, which is secreted by endothelial cells of the lymphatic capillary, binds heparan sulfates and forms gradients decaying into the interstitium. Despite the importance of CCL21 gradients, and chemokine gradients in general, the mechanisms of gradient formation are unclear. Studies on fibroblast growth factors have shown that limited diffusion is crucial for gradient formation. Here, we used the mouse dermis as a model tissue to address the necessity of CCL21 anchoring to lymphatic capillary heparan sulfates in the formation of interstitial CCL21 gradients. Surprisingly, the absence of lymphatic endothelial heparan sulfates resulted only in a modest decrease of CCL21 levels at the lymphatic capillaries and did neither affect interstitial CCL21 gradient shape nor dendritic cell migration toward lymphatic capillaries. Thus, heparan sulfates at the level of the lymphatic endothelium are dispensable for the formation of a functional CCL21 gradient."}],"volume":12,"project":[{"call_identifier":"H2020","grant_number":"724373","name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425"},{"_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","grant_number":"Y 564-B12","call_identifier":"FWF"}],"doi":"10.3389/fimmu.2021.630002","year":"2021","fulldoi":"https://doi.org/10.3389/fimmu.2021.630002","acknowledgement":"This work was supported by Sigrid Juselius fellowship (KV), University of Helsinki 3-year research grant (KV), Academy of Finland Research fellow funding (315710, to KV), the European Research Council (ERC CoG 724373 to MS), and by the Austrian Science foundation (FWF) (Y564-B12 START award to MS).\r\nTaija Mäkinen is acknowledged for providing Prox1CreERT2 transgenic mice and Yu Yamaguchi for providing the conditional Ext1 mouse strain.","day":"25","quality_controlled":"1","file_date_updated":"2021-03-22T12:08:26Z","author":[{"id":"368EE576-F248-11E8-B48F-1D18A9856A87","last_name":"Vaahtomeri","orcid":"0000-0001-7829-3518","first_name":"Kari","full_name":"Vaahtomeri, Kari"},{"id":"3356F664-F248-11E8-B48F-1D18A9856A87","last_name":"Moussion","first_name":"Christine","full_name":"Moussion, Christine"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","first_name":"Robert"},{"first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt"}],"type":"journal_article","publication_status":"published","month":"02","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"chicago":"Vaahtomeri, Kari, Christine Moussion, Robert Hauschild, and Michael K Sixt. “Shape and Function of Interstitial Chemokine CCL21 Gradients Are Independent of Heparan Sulfates Produced by Lymphatic Endothelium.” <i>Frontiers in Immunology</i>. Frontiers, 2021. <a href=\"https://doi.org/10.3389/fimmu.2021.630002\">https://doi.org/10.3389/fimmu.2021.630002</a>.","apa":"Vaahtomeri, K., Moussion, C., Hauschild, R., &#38; Sixt, M. K. (2021). Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium. <i>Frontiers in Immunology</i>. Frontiers. <a href=\"https://doi.org/10.3389/fimmu.2021.630002\">https://doi.org/10.3389/fimmu.2021.630002</a>","ieee":"K. Vaahtomeri, C. Moussion, R. Hauschild, and M. K. Sixt, “Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium,” <i>Frontiers in Immunology</i>, vol. 12. Frontiers, 2021.","ama":"Vaahtomeri K, Moussion C, Hauschild R, Sixt MK. Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium. <i>Frontiers in Immunology</i>. 2021;12. doi:<a href=\"https://doi.org/10.3389/fimmu.2021.630002\">10.3389/fimmu.2021.630002</a>","ista":"Vaahtomeri K, Moussion C, Hauschild R, Sixt MK. 2021. Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium. Frontiers in Immunology. 12, 630002.","short":"K. Vaahtomeri, C. Moussion, R. Hauschild, M.K. Sixt, Frontiers in Immunology 12 (2021).","mla":"Vaahtomeri, Kari, et al. “Shape and Function of Interstitial Chemokine CCL21 Gradients Are Independent of Heparan Sulfates Produced by Lymphatic Endothelium.” <i>Frontiers in Immunology</i>, vol. 12, 630002, Frontiers, 2021, doi:<a href=\"https://doi.org/10.3389/fimmu.2021.630002\">10.3389/fimmu.2021.630002</a>."},"article_processing_charge":"No","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2025-04-14T07:42:07Z","date_created":"2021-03-21T23:01:20Z","file":[{"file_size":3740146,"access_level":"open_access","file_id":"9277","checksum":"663f5a48375e42afa4bfef58d42ec186","content_type":"application/pdf","relation":"main_file","date_updated":"2021-03-22T12:08:26Z","date_created":"2021-03-22T12:08:26Z","file_name":"2021_FrontiersImmumo_Vaahtomeri.pdf","creator":"dernst","success":1}],"isi":1,"language":[{"iso":"eng"}],"ddc":["570"],"has_accepted_license":"1","status":"public"},{"month":"03","oa_version":"Published Version","citation":{"mla":"Gärtner, Florian R., and Michael K. Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>, vol. 56, no. 6, Elsevier, 2021, pp. 723–25, doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>.","ista":"Gärtner FR, Sixt MK. 2021. Engaging the front wheels to drive through fibrous terrain. Developmental Cell. 56(6), 723–725.","short":"F.R. Gärtner, M.K. Sixt, Developmental Cell 56 (2021) 723–725.","apa":"Gärtner, F. R., &#38; Sixt, M. K. (2021). Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>","ieee":"F. R. Gärtner and M. K. Sixt, “Engaging the front wheels to drive through fibrous terrain,” <i>Developmental Cell</i>, vol. 56, no. 6. Elsevier, pp. 723–725, 2021.","ama":"Gärtner FR, Sixt MK. Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. 2021;56(6):723-725. doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>","chicago":"Gärtner, Florian R, and Michael K Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","quality_controlled":"1","type":"journal_article","author":[{"full_name":"Gärtner, Florian R","first_name":"Florian R","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723"},{"first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"publication_status":"published","isi":1,"issue":"6","ddc":["570"],"language":[{"iso":"eng"}],"status":"public","date_created":"2021-03-28T22:01:41Z","date_updated":"2026-06-18T19:45:10Z","_id":"9294","oa":1,"department":[{"_id":"MiSi"}],"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"corr_author":"1","article_type":"original","date_published":"2021-03-22T00:00:00Z","intvolume":"        56","scopus_import":"1","title":"Engaging the front wheels to drive through fibrous terrain","abstract":[{"lang":"eng","text":"In this issue of Developmental Cell, Doyle and colleagues identify periodic anterior contraction as a characteristic feature of fibroblasts and mesenchymal cancer cells embedded in 3D collagen gels. This contractile mechanism generates a matrix prestrain required for crawling in fibrous 3D environments."}],"volume":56,"year":"2021","doi":"10.1016/j.devcel.2021.03.002","day":"22","fulldoi":"https://doi.org/10.1016/j.devcel.2021.03.002","pmid":1,"publisher":"Elsevier","external_id":{"isi":["000631681200004"],"pmid":["33756118"]},"publication":"Developmental Cell","page":"723-725","main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2021.03.002","open_access":"1"}]},{"volume":13,"project":[{"grant_number":"724373","call_identifier":"H2020","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients"}],"ec_funded":1,"abstract":[{"lang":"eng","text":"Attachment of adhesive molecules on cell culture surfaces to restrict cell adhesion to defined areas and shapes has been vital for the progress of in vitro research. In currently existing patterning methods, a combination of pattern properties such as stability, precision, specificity, high-throughput outcome, and spatiotemporal control is highly desirable but challenging to achieve. Here, we introduce a versatile and high-throughput covalent photoimmobilization technique, comprising a light-dose-dependent patterning step and a subsequent functionalization of the pattern via click chemistry. This two-step process is feasible on arbitrary surfaces and allows for generation of sustainable patterns and gradients. The method is validated in different biological systems by patterning adhesive ligands on cell-repellent surfaces, thereby constraining the growth and migration of cells to the designated areas. We then implement a sequential photopatterning approach by adding a second switchable patterning step, allowing for spatiotemporal control over two distinct surface patterns. As a proof of concept, we reconstruct the dynamics of the tip/stalk cell switch during angiogenesis. Our results show that the spatiotemporal control provided by our “sequential photopatterning” system is essential for mimicking dynamic biological processes and that our innovative approach has great potential for further applications in cell science."}],"fulldoi":"https://doi.org/10.1021/acsami.1c09850","acknowledgement":"We would like to thank Charlott Leu for the production of our chromium wafers, Louise Ritter for her contribution of the IF stainings in Figure 4, Shokoufeh Teymouri for her help with the Bioinert coated slides, and finally Prof. Dr. Joachim Rädler for his valuable scientific guidance.","day":"04","doi":"10.1021/acsami.1c09850","year":"2021","page":"35545–35560","publication":"ACS Applied Materials and Interfaces","external_id":{"isi":["000683741400026"],"pmid":["34283577"]},"publisher":"American Chemical Society","pmid":1,"_id":"9822","corr_author":"1","publication_identifier":{"eissn":["1944-8252"],"issn":["1944-8244"]},"oa":1,"department":[{"_id":"MiSi"},{"_id":"GaTk"},{"_id":"Bio"},{"_id":"CaGu"}],"date_published":"2021-08-04T00:00:00Z","article_type":"original","title":"Sequential and switchable patterning for studying cellular processes under spatiotemporal control","scopus_import":"1","intvolume":"        13","ddc":["620","570"],"language":[{"iso":"eng"}],"isi":1,"issue":"30","has_accepted_license":"1","status":"public","date_created":"2021-08-08T22:01:28Z","date_updated":"2025-07-10T12:02:02Z","file":[{"file_id":"9833","access_level":"open_access","file_size":7123293,"success":1,"file_name":"2021_ACSAppliedMaterialsAndInterfaces_Zisis.pdf","creator":"asandaue","relation":"main_file","date_updated":"2021-08-09T09:44:03Z","date_created":"2021-08-09T09:44:03Z","content_type":"application/pdf","checksum":"b043a91d9f9200e467b970b692687ed3"}],"citation":{"mla":"Zisis, Themistoklis, et al. “Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal Control.” <i>ACS Applied Materials and Interfaces</i>, vol. 13, no. 30, American Chemical Society, 2021, pp. 35545–35560, doi:<a href=\"https://doi.org/10.1021/acsami.1c09850\">10.1021/acsami.1c09850</a>.","short":"T. Zisis, J. Schwarz, M. Balles, M. Kretschmer, M. Nemethova, R.P. Chait, R. Hauschild, J. Lange, C.C. Guet, M.K. Sixt, S. Zahler, ACS Applied Materials and Interfaces 13 (2021) 35545–35560.","ista":"Zisis T, Schwarz J, Balles M, Kretschmer M, Nemethova M, Chait RP, Hauschild R, Lange J, Guet CC, Sixt MK, Zahler S. 2021. Sequential and switchable patterning for studying cellular processes under spatiotemporal control. ACS Applied Materials and Interfaces. 13(30), 35545–35560.","apa":"Zisis, T., Schwarz, J., Balles, M., Kretschmer, M., Nemethova, M., Chait, R. P., … Zahler, S. (2021). Sequential and switchable patterning for studying cellular processes under spatiotemporal control. <i>ACS Applied Materials and Interfaces</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acsami.1c09850\">https://doi.org/10.1021/acsami.1c09850</a>","ieee":"T. Zisis <i>et al.</i>, “Sequential and switchable patterning for studying cellular processes under spatiotemporal control,” <i>ACS Applied Materials and Interfaces</i>, vol. 13, no. 30. American Chemical Society, pp. 35545–35560, 2021.","ama":"Zisis T, Schwarz J, Balles M, et al. Sequential and switchable patterning for studying cellular processes under spatiotemporal control. <i>ACS Applied Materials and Interfaces</i>. 2021;13(30):35545–35560. doi:<a href=\"https://doi.org/10.1021/acsami.1c09850\">10.1021/acsami.1c09850</a>","chicago":"Zisis, Themistoklis, Jan Schwarz, Miriam Balles, Maibritt Kretschmer, Maria Nemethova, Remy P Chait, Robert Hauschild, et al. “Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal Control.” <i>ACS Applied Materials and Interfaces</i>. American Chemical Society, 2021. <a href=\"https://doi.org/10.1021/acsami.1c09850\">https://doi.org/10.1021/acsami.1c09850</a>."},"tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"oa_version":"Published Version","month":"08","article_processing_charge":"Yes (in subscription journal)","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2021-08-09T09:44:03Z","quality_controlled":"1","publication_status":"published","author":[{"last_name":"Zisis","first_name":"Themistoklis","full_name":"Zisis, Themistoklis"},{"first_name":"Jan","full_name":"Schwarz, Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","last_name":"Schwarz"},{"last_name":"Balles","full_name":"Balles, Miriam","first_name":"Miriam"},{"full_name":"Kretschmer, Maibritt","first_name":"Maibritt","last_name":"Kretschmer"},{"full_name":"Nemethova, Maria","first_name":"Maria","last_name":"Nemethova","id":"34E27F1C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Remy P","full_name":"Chait, Remy P","orcid":"0000-0003-0876-3187","id":"3464AE84-F248-11E8-B48F-1D18A9856A87","last_name":"Chait"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert"},{"first_name":"Janina","full_name":"Lange, Janina","last_name":"Lange"},{"id":"47F8433E-F248-11E8-B48F-1D18A9856A87","last_name":"Guet","orcid":"0000-0001-6220-2052","first_name":"Calin C","full_name":"Guet, Calin C"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-4561-241X","first_name":"Michael K","full_name":"Sixt, Michael K"},{"full_name":"Zahler, Stefan","first_name":"Stefan","last_name":"Zahler"}],"type":"journal_article"},{"file_date_updated":"2022-12-20T23:30:05Z","alternative_title":["ISTA Thesis"],"publication_status":"published","supervisor":[{"orcid":"0000-0002-4561-241X","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K"},{"first_name":"Calin C","full_name":"Guet, Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","last_name":"Guet","orcid":"0000-0001-6220-2052"}],"type":"dissertation","author":[{"orcid":"0000-0003-3768-877X","last_name":"Tomasek","id":"3AEC8556-F248-11E8-B48F-1D18A9856A87","full_name":"Tomasek, Kathrin","first_name":"Kathrin"}],"citation":{"ista":"Tomasek K. 2021. Pathogenic Escherichia coli hijack the host immune response. Institute of Science and Technology Austria.","short":"K. Tomasek, Pathogenic Escherichia Coli Hijack the Host Immune Response, Institute of Science and Technology Austria, 2021.","mla":"Tomasek, Kathrin. <i>Pathogenic Escherichia Coli Hijack the Host Immune Response</i>. Institute of Science and Technology Austria, 2021, doi:<a href=\"https://doi.org/10.15479/at:ista:10307\">10.15479/at:ista:10307</a>.","chicago":"Tomasek, Kathrin. “Pathogenic Escherichia Coli Hijack the Host Immune Response.” Institute of Science and Technology Austria, 2021. <a href=\"https://doi.org/10.15479/at:ista:10307\">https://doi.org/10.15479/at:ista:10307</a>.","ieee":"K. Tomasek, “Pathogenic Escherichia coli hijack the host immune response,” Institute of Science and Technology Austria, 2021.","apa":"Tomasek, K. (2021). <i>Pathogenic Escherichia coli hijack the host immune response</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:10307\">https://doi.org/10.15479/at:ista:10307</a>","ama":"Tomasek K. Pathogenic Escherichia coli hijack the host immune response. 2021. doi:<a href=\"https://doi.org/10.15479/at:ista:10307\">10.15479/at:ista:10307</a>"},"oa_version":"Published Version","month":"11","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_processing_charge":"No","date_updated":"2026-07-06T12:48:19Z","date_created":"2021-11-18T15:05:06Z","file":[{"file_id":"10308","access_level":"open_access","file_size":13266088,"file_name":"ThesisTomasekKathrin.pdf","creator":"ktomasek","date_updated":"2022-12-20T23:30:05Z","relation":"main_file","date_created":"2021-11-18T15:07:31Z","embargo":"2022-11-18","content_type":"application/pdf","checksum":"b39c9e0ef18d0484d537a67551effd02"},{"creator":"ktomasek","file_name":"ThesisTomasekKathrin.docx","relation":"source_file","date_updated":"2022-12-20T23:30:05Z","date_created":"2021-11-18T15:07:46Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"c0c440ee9e5ef1102a518a4f9f023e7c","file_id":"10309","embargo_to":"open_access","file_size":7539509,"access_level":"closed"}],"related_material":{"record":[{"id":"10316","relation":"part_of_dissertation","status":"public"}]},"ddc":["570"],"OA_place":"publisher","language":[{"iso":"eng"}],"degree_awarded":"PhD","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"EM-Fac"}],"status":"public","has_accepted_license":"1","date_published":"2021-11-18T00:00:00Z","title":"Pathogenic Escherichia coli hijack the host immune response","_id":"10307","publication_identifier":{"issn":["2663-337X"]},"corr_author":"1","oa":1,"department":[{"_id":"MiSi"},{"_id":"CaGu"},{"_id":"GradSch"}],"publisher":"Institute of Science and Technology Austria","page":"73","abstract":[{"text":"Bacteria-host interactions represent a continuous trade-off between benefit and risk. Thus, the host immune response is faced with a non-trivial problem – accommodate beneficial commensals and remove harmful pathogens. This is especially difficult as molecular patterns, such as lipopolysaccharide or specific surface organelles such as pili, are conserved in both, commensal and pathogenic bacteria. Type 1 pili, tightly regulated by phase variation, are considered an important virulence factor of pathogenic bacteria as they facilitate invasion into host cells. While invasion represents a de facto passive mechanism for pathogens to escape the host immune response, we demonstrate a fundamental role of type 1 pili as active modulators of the innate and adaptive immune response.","lang":"eng"}],"day":"18","fulldoi":"https://doi.org/10.15479/at:ista:10307","doi":"10.15479/at:ista:10307","year":"2021"},{"oa_version":"Preprint","citation":{"ama":"Tomasek K, Leithner AF, Glatzová I, Lukesch MS, Guet CC, Sixt MK. Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2021.10.18.464770\">10.1101/2021.10.18.464770</a>","ieee":"K. Tomasek, A. F. Leithner, I. Glatzová, M. S. Lukesch, C. C. Guet, and M. K. Sixt, “Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14,” <i>bioRxiv</i>. .","apa":"Tomasek, K., Leithner, A. F., Glatzová, I., Lukesch, M. S., Guet, C. C., &#38; Sixt, M. K. (n.d.). Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2021.10.18.464770\">https://doi.org/10.1101/2021.10.18.464770</a>","chicago":"Tomasek, Kathrin, Alexander F Leithner, Ivana Glatzová, Michael S. Lukesch, Calin C Guet, and Michael K Sixt. “Type 1 Piliated Uropathogenic Escherichia Coli Hijack the Host Immune Response by Binding to CD14.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2021.10.18.464770\">https://doi.org/10.1101/2021.10.18.464770</a>.","mla":"Tomasek, Kathrin, et al. “Type 1 Piliated Uropathogenic Escherichia Coli Hijack the Host Immune Response by Binding to CD14.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2021.10.18.464770\">10.1101/2021.10.18.464770</a>.","short":"K. Tomasek, A.F. Leithner, I. Glatzová, M.S. Lukesch, C.C. Guet, M.K. Sixt, BioRxiv (n.d.).","ista":"Tomasek K, Leithner AF, Glatzová I, Lukesch MS, Guet CC, Sixt MK. Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14. bioRxiv, <a href=\"https://doi.org/10.1101/2021.10.18.464770\">10.1101/2021.10.18.464770</a>."},"month":"10","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","publication_status":"draft","type":"preprint","author":[{"full_name":"Tomasek, Kathrin","first_name":"Kathrin","orcid":"0000-0003-3768-877X","last_name":"Tomasek","id":"3AEC8556-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Leithner, Alexander F","first_name":"Alexander F","orcid":"0000-0002-1073-744X","last_name":"Leithner","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Ivana","full_name":"Glatzová, Ivana","id":"727b3c7d-4939-11ec-89b3-b9b0750ab74d","last_name":"Glatzová"},{"first_name":"Michael S.","full_name":"Lukesch, Michael S.","last_name":"Lukesch"},{"first_name":"Calin C","full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","last_name":"Guet"},{"first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-4561-241X"}],"related_material":{"record":[{"id":"11843","relation":"later_version","status":"public"},{"id":"10307","relation":"dissertation_contains","status":"public"}]},"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"EM-Fac"}],"status":"public","date_updated":"2026-11-14T23:30:27Z","date_created":"2021-11-19T12:24:16Z","_id":"10316","corr_author":"1","department":[{"_id":"CaGu"},{"_id":"MiSi"}],"oa":1,"date_published":"2021-10-18T00:00:00Z","title":"Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14","project":[{"call_identifier":"H2020","grant_number":"724373","name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425"},{"_id":"26018E70-B435-11E9-9278-68D0E5697425","name":"Mechanical adaptation of lamellipodial actin","grant_number":"P29911","call_identifier":"FWF"}],"ec_funded":1,"abstract":[{"lang":"eng","text":"A key attribute of persistent or recurring bacterial infections is the ability of the pathogen to evade the host’s immune response. Many Enterobacteriaceae express type 1 pili, a pre-adapted virulence trait, to invade host epithelial cells and establish persistent infections. However, the molecular mechanisms and strategies by which bacteria actively circumvent the immune response of the host remain poorly understood. Here, we identified CD14, the major co-receptor for lipopolysaccharide detection, on dendritic cells as a previously undescribed binding partner of FimH, the protein located at the tip of the type 1 pilus of Escherichia coli. The FimH amino acids involved in CD14 binding are highly conserved across pathogenic and non-pathogenic strains. Binding of pathogenic bacteria to CD14 lead to reduced dendritic cell migration and blunted expression of co-stimulatory molecules, both rate-limiting factors of T cell activation. While defining an active molecular mechanism of immune evasion by pathogens, the interaction between FimH and CD14 represents a potential target to interfere with persistent and recurrent infections, such as urinary tract infections or Crohn’s disease."}],"day":"18","acknowledgement":"We thank Ulrich Dobrindt for providing UPEC strain CFT073, Vlad Gavra and Maximilian Götz, Bor Kavčič, Jonna Alanko and Eva Kiermaier for help with experiments and Robert Hauschild, Julian Stopp and Saren Tasciyan for help with data analysis. We thank the IST Austria Scientific Service Units, especially the Bioimaging facility, the Preclinical facility and the Electron microscopy facility for technical support, Jakob Wallner and all members of the Guet and Sixt lab for fruitful discussions and Daria Siekhaus for critically reading the manuscript. This work was supported by grants from the Austrian Research Promotion Agency (FEMtech 868984) to I.G., the European Research Council (CoG 724373) and the Austrian Science Fund (FWF P29911) to M.S.","fulldoi":"https://doi.org/10.1101/2021.10.18.464770","das_tickbox":"1","year":"2021","doi":"10.1101/2021.10.18.464770","publication":"bioRxiv","main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/2021.10.18.464770v1"}]},{"date_published":"2021-05-24T00:00:00Z","article_type":"original","scopus_import":"1","title":"Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development","intvolume":"        12","article_number":"3058","_id":"9429","department":[{"_id":"GaNo"},{"_id":"JoDa"},{"_id":"FlSc"},{"_id":"MiSi"},{"_id":"LifeSc"},{"_id":"Bio"}],"oa":1,"corr_author":"1","publication_identifier":{"eissn":["2041-1723"]},"publication":"Nature Communications","external_id":{"isi":["000658769900010"]},"publisher":"Springer Nature","abstract":[{"text":"De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3 lead to autism spectrum disorder (ASD). In mouse, constitutive haploinsufficiency leads to motor coordination deficits as well as ASD-relevant social and cognitive impairments. However, induction of Cul3 haploinsufficiency later in life does not lead to ASD-relevant behaviors, pointing to an important role of Cul3 during a critical developmental window. Here we show that Cul3 is essential to regulate neuronal migration and, therefore, constitutive Cul3 heterozygous mutant mice display cortical lamination abnormalities. At the molecular level, we found that Cul3 controls neuronal migration by tightly regulating the amount of Plastin3 (Pls3), a previously unrecognized player of neural migration. Furthermore, we found that Pls3 cell-autonomously regulates cell migration by regulating actin cytoskeleton organization, and its levels are inversely proportional to neural migration speed. Finally, we provide evidence that cellular phenotypes associated with autism-linked gene haploinsufficiency can be rescued by transcriptional activation of the intact allele in vitro, offering a proof of concept for a potential therapeutic approach for ASDs.","lang":"eng"}],"ec_funded":1,"volume":12,"project":[{"call_identifier":"H2020","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"_id":"25444568-B435-11E9-9278-68D0E5697425","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","grant_number":"715508","call_identifier":"H2020"},{"call_identifier":"FWF","grant_number":"W1232","name":"Molecular Drug Targets","_id":"2548AE96-B435-11E9-9278-68D0E5697425"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P07-Neural stem cells in autism and epilepsy","_id":"05A0D778-7A3F-11EA-A408-12923DDC885E","grant_number":"F7807"},{"_id":"265CB4D0-B435-11E9-9278-68D0E5697425","name":"Optical control of synaptic function via adhesion molecules","grant_number":"I03600","call_identifier":"FWF"}],"year":"2021","doi":"10.1038/s41467-021-23123-x","keyword":["General Biochemistry","Genetics and Molecular Biology"],"fulldoi":"https://doi.org/10.1038/s41467-021-23123-x","acknowledgement":"We thank A. Coll Manzano, F. Freeman, M. Ladron de Guevara, and A. Ç. Yahya for technical assistance, S. Deixler, A. Lepold, and A. Schlerka for the management of our animal colony, as well as M. Schunn and the Preclinical Facility team for technical assistance. We thank K. Heesom and her team at the University of Bristol Proteomics Facility for the proteomics sample preparation, data generation, and analysis support. We thank Y. B. Simon for kindly providing the plasmid for lentiviral labeling. Further, we thank M. Sixt for his advice regarding cell migration and the fruitful discussions. This work was supported by the ISTPlus postdoctoral fellowship (Grant Agreement No. 754411) to B.B., by the European Union’s Horizon 2020 research and innovation program (ERC) grant 715508 (REVERSEAUTISM), and by the Austrian Science Fund (FWF) to G.N. (DK W1232-B24 and SFB F7807-B) and to J.G.D (I3600-B27).","day":"24","quality_controlled":"1","file_date_updated":"2021-05-28T12:39:43Z","author":[{"first_name":"Jasmin","full_name":"Morandell, Jasmin","id":"4739D480-F248-11E8-B48F-1D18A9856A87","last_name":"Morandell"},{"first_name":"Lena A","full_name":"Schwarz, Lena A","id":"29A8453C-F248-11E8-B48F-1D18A9856A87","last_name":"Schwarz"},{"orcid":"0000-0003-1843-3173","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","last_name":"Basilico","first_name":"Bernadette","full_name":"Basilico, Bernadette"},{"orcid":"0000-0003-1671-393X","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","last_name":"Tasciyan","first_name":"Saren","full_name":"Tasciyan, Saren"},{"orcid":"0000-0001-8370-6161","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","last_name":"Dimchev","first_name":"Georgi A","full_name":"Dimchev, Georgi A"},{"first_name":"Armel","full_name":"Nicolas, Armel","id":"2A103192-F248-11E8-B48F-1D18A9856A87","last_name":"Nicolas"},{"orcid":"0000-0003-1216-9105","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M","first_name":"Christoph M"},{"id":"382077BA-F248-11E8-B48F-1D18A9856A87","last_name":"Kreuzinger","first_name":"Caroline","full_name":"Kreuzinger, Caroline"},{"full_name":"Dotter, Christoph","first_name":"Christoph","last_name":"Dotter","id":"4C66542E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9033-9096"},{"first_name":"Lisa","full_name":"Knaus, Lisa","id":"3B2ABCF4-F248-11E8-B48F-1D18A9856A87","last_name":"Knaus"},{"first_name":"Zoe","full_name":"Dobler, Zoe","id":"D23090A2-9057-11EA-883A-A8396FC7A38F","last_name":"Dobler"},{"first_name":"Emanuele","full_name":"Cacci, Emanuele","last_name":"Cacci"},{"full_name":"Schur, Florian KM","first_name":"Florian KM","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4790-8078"},{"orcid":"0000-0001-8559-3973","last_name":"Danzl","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","full_name":"Danzl, Johann G","first_name":"Johann G"},{"id":"3E57A680-F248-11E8-B48F-1D18A9856A87","last_name":"Novarino","orcid":"0000-0002-7673-7178","first_name":"Gaia","full_name":"Novarino, Gaia"}],"type":"journal_article","publication_status":"published","month":"05","citation":{"chicago":"Morandell, Jasmin, Lena A Schwarz, Bernadette Basilico, Saren Tasciyan, Georgi A Dimchev, Armel Nicolas, Christoph M Sommer, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-23123-x\">https://doi.org/10.1038/s41467-021-23123-x</a>.","ama":"Morandell J, Schwarz LA, Basilico B, et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>Nature Communications</i>. 2021;12(1). doi:<a href=\"https://doi.org/10.1038/s41467-021-23123-x\">10.1038/s41467-021-23123-x</a>","ieee":"J. Morandell <i>et al.</i>, “Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development,” <i>Nature Communications</i>, vol. 12, no. 1. Springer Nature, 2021.","apa":"Morandell, J., Schwarz, L. A., Basilico, B., Tasciyan, S., Dimchev, G. A., Nicolas, A., … Novarino, G. (2021). Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-23123-x\">https://doi.org/10.1038/s41467-021-23123-x</a>","ista":"Morandell J, Schwarz LA, Basilico B, Tasciyan S, Dimchev GA, Nicolas A, Sommer CM, Kreuzinger C, Dotter C, Knaus L, Dobler Z, Cacci E, Schur FK, Danzl JG, Novarino G. 2021. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nature Communications. 12(1), 3058.","short":"J. Morandell, L.A. Schwarz, B. Basilico, S. Tasciyan, G.A. Dimchev, A. Nicolas, C.M. Sommer, C. Kreuzinger, C. Dotter, L. Knaus, Z. Dobler, E. Cacci, F.K. Schur, J.G. Danzl, G. Novarino, Nature Communications 12 (2021).","mla":"Morandell, Jasmin, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>Nature Communications</i>, vol. 12, no. 1, 3058, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-23123-x\">10.1038/s41467-021-23123-x</a>."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","article_processing_charge":"No","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_created":"2021-05-28T11:49:46Z","date_updated":"2026-11-14T23:31:21Z","file":[{"access_level":"open_access","file_size":9358599,"file_id":"9430","checksum":"337e0f7959c35ec959984cacdcb472ba","content_type":"application/pdf","relation":"main_file","date_created":"2021-05-28T12:39:43Z","date_updated":"2021-05-28T12:39:43Z","success":1,"file_name":"2021_NatureCommunications_Morandell.pdf","creator":"kschuh"}],"issue":"1","isi":1,"related_material":{"record":[{"status":"public","id":"7800","relation":"earlier_version"},{"status":"public","relation":"dissertation_contains","id":"12401"},{"status":"public","relation":"dissertation_contains","id":"19557"}],"link":[{"url":"https://ist.ac.at/en/news/defective-gene-slows-down-brain-cells/","relation":"press_release"}]},"ddc":["572"],"language":[{"iso":"eng"}],"has_accepted_license":"1","status":"public","acknowledged_ssus":[{"_id":"PreCl"}]},{"_id":"7623","department":[{"_id":"MiSi"}],"oa":1,"publication_identifier":{"issn":["0218-2025"]},"article_type":"original","date_published":"2020-03-18T00:00:00Z","intvolume":"        30","scopus_import":"1","title":"Modeling adhesion-independent cell migration","abstract":[{"text":"A two-dimensional mathematical model for cells migrating without adhesion capabilities is presented and analyzed. Cells are represented by their cortex, which is modeled as an elastic curve, subject to an internal pressure force. Net polymerization or depolymerization in the cortex is modeled via local addition or removal of material, driving a cortical flow. The model takes the form of a fully nonlinear degenerate parabolic system. An existence analysis is carried out by adapting ideas from the theory of gradient flows. Numerical simulations show that these simple rules can account for the behavior observed in experiments, suggesting a possible mechanical mechanism for adhesion-independent motility.","lang":"eng"}],"project":[{"name":"Modeling of Polarization and Motility of Leukocytes in Three-Dimensional Environments","_id":"25AD6156-B435-11E9-9278-68D0E5697425","grant_number":"LS13-029"}],"volume":30,"doi":"10.1142/S021820252050013X","year":"2020","acknowledgement":"This work has been supported by the Vienna Science and Technology Fund, Grant no. LS13-029. G.J. and C.S. also acknowledge support by the Austrian Science Fund, Grants no. W1245, F 65, and W1261, as well as by the Fondation Sciences Mathématiques de Paris, and by Paris-Sciences-et-Lettres.","day":"18","fulldoi":"https://doi.org/10.1142/S021820252050013X","publisher":"World Scientific Publishing","arxiv":1,"publication":"Mathematical Models and Methods in Applied Sciences","page":"513-537","external_id":{"isi":["000525349900003"],"arxiv":["1903.09426"]},"main_file_link":[{"url":"https://arxiv.org/abs/1903.09426","open_access":"1"}],"month":"03","citation":{"chicago":"Jankowiak, Gaspard, Diane Peurichard, Anne Reversat, Christian Schmeiser, and Michael K Sixt. “Modeling Adhesion-Independent Cell Migration.” <i>Mathematical Models and Methods in Applied Sciences</i>. World Scientific Publishing, 2020. <a href=\"https://doi.org/10.1142/S021820252050013X\">https://doi.org/10.1142/S021820252050013X</a>.","ama":"Jankowiak G, Peurichard D, Reversat A, Schmeiser C, Sixt MK. Modeling adhesion-independent cell migration. <i>Mathematical Models and Methods in Applied Sciences</i>. 2020;30(3):513-537. doi:<a href=\"https://doi.org/10.1142/S021820252050013X\">10.1142/S021820252050013X</a>","ieee":"G. Jankowiak, D. Peurichard, A. Reversat, C. Schmeiser, and M. K. Sixt, “Modeling adhesion-independent cell migration,” <i>Mathematical Models and Methods in Applied Sciences</i>, vol. 30, no. 3. World Scientific Publishing, pp. 513–537, 2020.","apa":"Jankowiak, G., Peurichard, D., Reversat, A., Schmeiser, C., &#38; Sixt, M. K. (2020). Modeling adhesion-independent cell migration. <i>Mathematical Models and Methods in Applied Sciences</i>. World Scientific Publishing. <a href=\"https://doi.org/10.1142/S021820252050013X\">https://doi.org/10.1142/S021820252050013X</a>","ista":"Jankowiak G, Peurichard D, Reversat A, Schmeiser C, Sixt MK. 2020. Modeling adhesion-independent cell migration. Mathematical Models and Methods in Applied Sciences. 30(3), 513–537.","short":"G. Jankowiak, D. Peurichard, A. Reversat, C. Schmeiser, M.K. Sixt, Mathematical Models and Methods in Applied Sciences 30 (2020) 513–537.","mla":"Jankowiak, Gaspard, et al. “Modeling Adhesion-Independent Cell Migration.” <i>Mathematical Models and Methods in Applied Sciences</i>, vol. 30, no. 3, World Scientific Publishing, 2020, pp. 513–37, doi:<a href=\"https://doi.org/10.1142/S021820252050013X\">10.1142/S021820252050013X</a>."},"oa_version":"Preprint","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_processing_charge":"No","quality_controlled":"1","type":"journal_article","author":[{"last_name":"Jankowiak","first_name":"Gaspard","full_name":"Jankowiak, Gaspard"},{"first_name":"Diane","full_name":"Peurichard, Diane","last_name":"Peurichard"},{"first_name":"Anne","full_name":"Reversat, Anne","id":"35B76592-F248-11E8-B48F-1D18A9856A87","last_name":"Reversat","orcid":"0000-0003-0666-8928"},{"full_name":"Schmeiser, Christian","first_name":"Christian","last_name":"Schmeiser"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"}],"publication_status":"published","issue":"3","isi":1,"language":[{"iso":"eng"}],"status":"public","date_created":"2020-03-31T11:25:05Z","date_updated":"2026-04-16T09:35:31Z"},{"ec_funded":1,"abstract":[{"text":"Cells navigating through complex tissues face a fundamental challenge: while multiple protrusions explore different paths, the cell needs to avoid entanglement. How a cell surveys and then corrects its own shape is poorly understood. Here, we demonstrate that spatially distinct microtubule dynamics regulate amoeboid cell migration by locally promoting the retraction of protrusions. In migrating dendritic cells, local microtubule depolymerization within protrusions remote from the microtubule organizing center triggers actomyosin contractility controlled by RhoA and its exchange factor Lfc. Depletion of Lfc leads to aberrant myosin localization, thereby causing two effects that rate-limit locomotion: (1) impaired cell edge coordination during path finding and (2) defective adhesion resolution. Compromised shape control is particularly hindering in geometrically complex microenvironments, where it leads to entanglement and ultimately fragmentation of the cell body. We thus demonstrate that microtubules can act as a proprioceptive device: they sense cell shape and control actomyosin retraction to sustain cellular coherence.","lang":"eng"}],"project":[{"call_identifier":"FP7","grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","_id":"25A603A2-B435-11E9-9278-68D0E5697425"},{"name":"Cellular Navigation Along Spatial Gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"724373"},{"grant_number":"P29911","call_identifier":"FWF","_id":"26018E70-B435-11E9-9278-68D0E5697425","name":"Mechanical adaptation of lamellipodial actin"},{"_id":"252C3B08-B435-11E9-9278-68D0E5697425","name":"Nano-Analytics of Cellular Systems","grant_number":"W1250-B20","call_identifier":"FWF"},{"name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"291734"},{"grant_number":"ALTF 1396-2014","name":"Molecular and system level view of immune cell migration","_id":"25A48D24-B435-11E9-9278-68D0E5697425"}],"volume":219,"year":"2020","doi":"10.1083/jcb.201907154","acknowledgement":"The authors thank the Scientific Service Units (Life Sciences, Bioimaging, Preclinical) of the Institute of Science and Technology Austria for excellent support. This work was funded by the European Research Council (ERC StG 281556 and CoG 724373), two grants from the Austrian\r\nScience Fund (FWF; P29911 and DK Nanocell W1250-B20 to M. Sixt) and by the German Research Foundation (DFG SFB1032 project B09) to O. Thorn-Seshold and D. Trauner. J. Renkawitz was supported by ISTFELLOW funding from the People Program (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under the Research Executive Agency grant agreement (291734) and a European Molecular Biology Organization long-term fellowship (ALTF 1396-2014) co-funded by the European Commission (LTFCOFUND2013, GA-2013-609409), E. Kiermaier by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy—EXC 2151—390873048, and H. Hacker by the American Lebanese Syrian Associated ¨Charities. K.-D. Fischer was supported by the Analysis, Imaging and Modelling of Neuronal and Inflammatory Processes graduate school funded by the Ministry of Economics, Science, and Digitisation of the State Saxony-Anhalt and by the European Funds for Social and Regional Development.","day":"01","fulldoi":"https://doi.org/10.1083/jcb.201907154","pmid":1,"publisher":"Rockefeller University Press","external_id":{"pmid":["32379884"],"isi":["000538141100020"]},"publication":"The Journal of Cell Biology","_id":"7875","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"oa":1,"publication_identifier":{"eissn":["1540-8140"]},"corr_author":"1","article_type":"original","date_published":"2020-06-01T00:00:00Z","intvolume":"       219","article_number":"e201907154","scopus_import":"1","title":"Microtubules control cellular shape and coherence in amoeboid migrating cells","isi":1,"issue":"6","language":[{"iso":"eng"}],"ddc":["570"],"status":"public","has_accepted_license":"1","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"PreCl"}],"date_updated":"2025-04-14T13:10:03Z","date_created":"2020-05-24T22:00:56Z","file":[{"access_level":"open_access","file_size":7536712,"file_id":"8801","checksum":"cb0b9c77842ae1214caade7b77e4d82d","content_type":"application/pdf","relation":"main_file","date_updated":"2020-11-24T13:25:13Z","date_created":"2020-11-24T13:25:13Z","success":1,"creator":"dernst","file_name":"2020_JCellBiol_Kopf.pdf"}],"month":"06","citation":{"ama":"Kopf A, Renkawitz J, Hauschild R, et al. Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. 2020;219(6). doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>","ieee":"A. Kopf <i>et al.</i>, “Microtubules control cellular shape and coherence in amoeboid migrating cells,” <i>The Journal of Cell Biology</i>, vol. 219, no. 6. Rockefeller University Press, 2020.","apa":"Kopf, A., Renkawitz, J., Hauschild, R., Girkontaite, I., Tedford, K., Merrin, J., … Sixt, M. K. (2020). Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>","chicago":"Kopf, Aglaja, Jörg Renkawitz, Robert Hauschild, Irute Girkontaite, Kerry Tedford, Jack Merrin, Oliver Thorn-Seshold, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>. Rockefeller University Press, 2020. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>.","mla":"Kopf, Aglaja, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>, vol. 219, no. 6, e201907154, Rockefeller University Press, 2020, doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>.","short":"A. Kopf, J. Renkawitz, R. Hauschild, I. Girkontaite, K. Tedford, J. Merrin, O. Thorn-Seshold, D. Trauner, H. Häcker, K.D. Fischer, E. Kiermaier, M.K. Sixt, The Journal of Cell Biology 219 (2020).","ista":"Kopf A, Renkawitz J, Hauschild R, Girkontaite I, Tedford K, Merrin J, Thorn-Seshold O, Trauner D, Häcker H, Fischer KD, Kiermaier E, Sixt MK. 2020. Microtubules control cellular shape and coherence in amoeboid migrating cells. The Journal of Cell Biology. 219(6), e201907154."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","quality_controlled":"1","file_date_updated":"2020-11-24T13:25:13Z","type":"journal_article","author":[{"first_name":"Aglaja","full_name":"Kopf, Aglaja","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","last_name":"Kopf","orcid":"0000-0002-2187-6656"},{"full_name":"Renkawitz, Jörg","first_name":"Jörg","orcid":"0000-0003-2856-3369","last_name":"Renkawitz","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Robert","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"last_name":"Girkontaite","first_name":"Irute","full_name":"Girkontaite, Irute"},{"full_name":"Tedford, Kerry","first_name":"Kerry","last_name":"Tedford"},{"full_name":"Merrin, Jack","first_name":"Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Thorn-Seshold","full_name":"Thorn-Seshold, Oliver","first_name":"Oliver"},{"last_name":"Trauner","id":"E8F27F48-3EBA-11E9-92A1-B709E6697425","full_name":"Trauner, Dirk","first_name":"Dirk"},{"last_name":"Häcker","first_name":"Hans","full_name":"Häcker, Hans"},{"first_name":"Klaus Dieter","full_name":"Fischer, Klaus Dieter","last_name":"Fischer"},{"last_name":"Kiermaier","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6165-5738","full_name":"Kiermaier, Eva","first_name":"Eva"},{"first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"publication_status":"published"},{"date_created":"2020-05-24T22:00:57Z","date_updated":"2026-06-18T19:27:52Z","OA_place":"publisher","language":[{"iso":"eng"}],"ddc":["570"],"issue":"5","isi":1,"status":"public","quality_controlled":"1","publication_status":"published","type":"journal_article","author":[{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"last_name":"Lämmermann","full_name":"Lämmermann, Tim","first_name":"Tim"}],"oa_version":"Published Version","citation":{"chicago":"Sixt, Michael K, and Tim Lämmermann. “T Cells: Bridge-and-Channel Commute to the White Pulp.” <i>Immunity</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.immuni.2020.04.020\">https://doi.org/10.1016/j.immuni.2020.04.020</a>.","apa":"Sixt, M. K., &#38; Lämmermann, T. (2020). T cells: Bridge-and-channel commute to the white pulp. <i>Immunity</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.immuni.2020.04.020\">https://doi.org/10.1016/j.immuni.2020.04.020</a>","ieee":"M. K. Sixt and T. Lämmermann, “T cells: Bridge-and-channel commute to the white pulp,” <i>Immunity</i>, vol. 52, no. 5. Elsevier, pp. 721–723, 2020.","ama":"Sixt MK, Lämmermann T. T cells: Bridge-and-channel commute to the white pulp. <i>Immunity</i>. 2020;52(5):721-723. doi:<a href=\"https://doi.org/10.1016/j.immuni.2020.04.020\">10.1016/j.immuni.2020.04.020</a>","short":"M.K. Sixt, T. Lämmermann, Immunity 52 (2020) 721–723.","ista":"Sixt MK, Lämmermann T. 2020. T cells: Bridge-and-channel commute to the white pulp. Immunity. 52(5), 721–723.","mla":"Sixt, Michael K., and Tim Lämmermann. “T Cells: Bridge-and-Channel Commute to the White Pulp.” <i>Immunity</i>, vol. 52, no. 5, Elsevier, 2020, pp. 721–23, doi:<a href=\"https://doi.org/10.1016/j.immuni.2020.04.020\">10.1016/j.immuni.2020.04.020</a>."},"month":"05","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","publisher":"Elsevier","page":"721-723","publication":"Immunity","external_id":{"pmid":["32433942"],"isi":["000535371100002"]},"pmid":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.immuni.2020.04.020"}],"volume":52,"abstract":[{"text":"In contrast to lymph nodes, the lymphoid regions of the spleen—the white pulp—are located deep within the organ, yielding the trafficking paths of T cells in the white pulp largely invisible. In an intravital microscopy tour de force reported in this issue of Immunity, Chauveau et al. show that T cells perform unidirectional, perivascular migration through the enigmatic marginal zone bridging channels. ","lang":"eng"}],"day":"19","OA_type":"free access","fulldoi":"https://doi.org/10.1016/j.immuni.2020.04.020","year":"2020","doi":"10.1016/j.immuni.2020.04.020","article_type":"original","date_published":"2020-05-19T00:00:00Z","intvolume":"        52","scopus_import":"1","title":"T cells: Bridge-and-channel commute to the white pulp","_id":"7876","publication_identifier":{"eissn":["1097-4180"],"issn":["1074-7613"]},"department":[{"_id":"MiSi"}],"oa":1},{"isi":1,"language":[{"iso":"eng"}],"ddc":["570"],"has_accepted_license":"1","status":"public","date_created":"2020-05-31T22:00:49Z","date_updated":"2026-04-02T14:32:12Z","file":[{"file_name":"2020_eLife_Damiano_Guercio.pdf","creator":"dernst","date_created":"2020-06-02T10:35:37Z","date_updated":"2020-07-14T12:48:05Z","relation":"main_file","content_type":"application/pdf","checksum":"d33bd4441b9a0195718ce1ba5d2c48a6","file_id":"7914","file_size":10535713,"access_level":"open_access"}],"month":"05","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"chicago":"Damiano-Guercio, Julia, Laëtitia Kurzawa, Jan Müller, Georgi A Dimchev, Matthias Schaks, Maria Nemethova, Thomas Pokrant, et al. “Loss of Ena/VASP Interferes with Lamellipodium Architecture, Motility and Integrin-Dependent Adhesion.” <i>ELife</i>. eLife Sciences Publications, 2020. <a href=\"https://doi.org/10.7554/eLife.55351\">https://doi.org/10.7554/eLife.55351</a>.","ama":"Damiano-Guercio J, Kurzawa L, Müller J, et al. Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion. <i>eLife</i>. 2020;9. doi:<a href=\"https://doi.org/10.7554/eLife.55351\">10.7554/eLife.55351</a>","ieee":"J. Damiano-Guercio <i>et al.</i>, “Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion,” <i>eLife</i>, vol. 9. eLife Sciences Publications, 2020.","apa":"Damiano-Guercio, J., Kurzawa, L., Müller, J., Dimchev, G. A., Schaks, M., Nemethova, M., … Faix, J. (2020). Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.55351\">https://doi.org/10.7554/eLife.55351</a>","ista":"Damiano-Guercio J, Kurzawa L, Müller J, Dimchev GA, Schaks M, Nemethova M, Pokrant T, Brühmann S, Linkner J, Blanchoin L, Sixt MK, Rottner K, Faix J. 2020. Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion. eLife. 9, e55351.","short":"J. Damiano-Guercio, L. Kurzawa, J. Müller, G.A. Dimchev, M. Schaks, M. Nemethova, T. Pokrant, S. Brühmann, J. Linkner, L. Blanchoin, M.K. Sixt, K. Rottner, J. Faix, ELife 9 (2020).","mla":"Damiano-Guercio, Julia, et al. “Loss of Ena/VASP Interferes with Lamellipodium Architecture, Motility and Integrin-Dependent Adhesion.” <i>ELife</i>, vol. 9, e55351, eLife Sciences Publications, 2020, doi:<a href=\"https://doi.org/10.7554/eLife.55351\">10.7554/eLife.55351</a>."},"article_processing_charge":"No","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","quality_controlled":"1","file_date_updated":"2020-07-14T12:48:05Z","author":[{"full_name":"Damiano-Guercio, Julia","first_name":"Julia","last_name":"Damiano-Guercio"},{"last_name":"Kurzawa","full_name":"Kurzawa, Laëtitia","first_name":"Laëtitia"},{"full_name":"Müller, Jan","first_name":"Jan","last_name":"Müller","id":"AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D"},{"id":"38C393BE-F248-11E8-B48F-1D18A9856A87","last_name":"Dimchev","orcid":"0000-0001-8370-6161","first_name":"Georgi A","full_name":"Dimchev, Georgi A"},{"full_name":"Schaks, Matthias","first_name":"Matthias","last_name":"Schaks"},{"first_name":"Maria","full_name":"Nemethova, Maria","id":"34E27F1C-F248-11E8-B48F-1D18A9856A87","last_name":"Nemethova"},{"full_name":"Pokrant, Thomas","first_name":"Thomas","last_name":"Pokrant"},{"full_name":"Brühmann, Stefan","first_name":"Stefan","last_name":"Brühmann"},{"last_name":"Linkner","full_name":"Linkner, Joern","first_name":"Joern"},{"full_name":"Blanchoin, Laurent","first_name":"Laurent","last_name":"Blanchoin"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"first_name":"Klemens","full_name":"Rottner, Klemens","last_name":"Rottner"},{"full_name":"Faix, Jan","first_name":"Jan","last_name":"Faix"}],"type":"journal_article","publication_status":"published","ec_funded":1,"abstract":[{"lang":"eng","text":"Cell migration entails networks and bundles of actin filaments termed lamellipodia and microspikes or filopodia, respectively, as well as focal adhesions, all of which recruit Ena/VASP family members hitherto thought to antagonize efficient cell motility. However, we find these proteins to act as positive regulators of migration in different murine cell lines. CRISPR/Cas9-mediated loss of Ena/VASP proteins reduced lamellipodial actin assembly and perturbed lamellipodial architecture, as evidenced by changed network geometry as well as reduction of filament length and number that was accompanied by abnormal Arp2/3 complex and heterodimeric capping protein accumulation. Loss of Ena/VASP function also abolished the formation of microspikes normally embedded in lamellipodia, but not of filopodia capable of emanating without lamellipodia. Ena/VASP-deficiency also impaired integrin-mediated adhesion accompanied by reduced traction forces exerted through these structures. Our data thus uncover novel Ena/VASP functions of these actin polymerases that are fully consistent with their promotion of cell migration."}],"volume":9,"project":[{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients","grant_number":"724373","call_identifier":"H2020"}],"doi":"10.7554/eLife.55351","year":"2020","fulldoi":"https://doi.org/10.7554/eLife.55351","day":"11","pmid":1,"external_id":{"pmid":["32391788"],"isi":["000537208000001"]},"publication":"eLife","publisher":"eLife Sciences Publications","_id":"7909","department":[{"_id":"MiSi"}],"oa":1,"publication_identifier":{"eissn":["2050-084X"]},"date_published":"2020-05-11T00:00:00Z","article_type":"original","scopus_import":"1","title":"Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion","intvolume":"         9","article_number":"e55351"},{"_id":"8132","publication_identifier":{"eissn":["2470-9468"]},"department":[{"_id":"MiSi"}],"oa":1,"article_type":"original","date_published":"2020-07-10T00:00:00Z","intvolume":"         5","article_number":"eabc3979","title":"The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity","scopus_import":"1","volume":5,"abstract":[{"text":"The WAVE regulatory complex (WRC) is crucial for assembly of the peripheral branched actin network constituting one of the main drivers of eukaryotic cell migration. Here, we uncover an essential role of the hematopoietic-specific WRC component HEM1 for immune cell development. Germline-encoded HEM1 deficiency underlies an inborn error of immunity with systemic autoimmunity, at cellular level marked by WRC destabilization, reduced filamentous actin, and failure to assemble lamellipodia. Hem1−/− mice display systemic autoimmunity, phenocopying the human disease. In the absence of Hem1, B cells become deprived of extracellular stimuli necessary to maintain the strength of B cell receptor signaling at a level permissive for survival of non-autoreactive B cells. This shifts the balance of B cell fate choices toward autoreactive B cells and thus autoimmunity.","lang":"eng"}],"day":"10","OA_type":"green","fulldoi":"https://doi.org/10.1126/sciimmunol.abc3979","year":"2020","doi":"10.1126/sciimmunol.abc3979","publisher":"AAAS","publication":"Science Immunology","external_id":{"isi":["000546994600004"],"pmid":["32646852"]},"pmid":1,"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7116756"}],"oa_version":"Submitted Version","citation":{"short":"E. Salzer, S. Zoghi, M.G. Kiss, F. Kage, C. Rashkova, S. Stahnke, M. Haimel, R. Platzer, M. Caldera, R.C. Ardy, B. Hoeger, J. Block, D. Medgyesi, C. Sin, S. Shahkarami, R. Kain, V. Ziaee, P. Hammerl, C. Bock, J. Menche, L. Dupré, J.B. Huppa, M.K. Sixt, A. Lomakin, K. Rottner, C.J. Binder, T.E.B. Stradal, N. Rezaei, K. Boztug, Science Immunology 5 (2020).","ista":"Salzer E, Zoghi S, Kiss MG, Kage F, Rashkova C, Stahnke S, Haimel M, Platzer R, Caldera M, Ardy RC, Hoeger B, Block J, Medgyesi D, Sin C, Shahkarami S, Kain R, Ziaee V, Hammerl P, Bock C, Menche J, Dupré L, Huppa JB, Sixt MK, Lomakin A, Rottner K, Binder CJ, Stradal TEB, Rezaei N, Boztug K. 2020. The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity. Science Immunology. 5(49), eabc3979.","mla":"Salzer, Elisabeth, et al. “The Cytoskeletal Regulator HEM1 Governs B Cell Development and Prevents Autoimmunity.” <i>Science Immunology</i>, vol. 5, no. 49, eabc3979, AAAS, 2020, doi:<a href=\"https://doi.org/10.1126/sciimmunol.abc3979\">10.1126/sciimmunol.abc3979</a>.","chicago":"Salzer, Elisabeth, Samaneh Zoghi, Máté G. Kiss, Frieda Kage, Christina Rashkova, Stephanie Stahnke, Matthias Haimel, et al. “The Cytoskeletal Regulator HEM1 Governs B Cell Development and Prevents Autoimmunity.” <i>Science Immunology</i>. AAAS, 2020. <a href=\"https://doi.org/10.1126/sciimmunol.abc3979\">https://doi.org/10.1126/sciimmunol.abc3979</a>.","ieee":"E. Salzer <i>et al.</i>, “The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity,” <i>Science Immunology</i>, vol. 5, no. 49. AAAS, 2020.","apa":"Salzer, E., Zoghi, S., Kiss, M. G., Kage, F., Rashkova, C., Stahnke, S., … Boztug, K. (2020). The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity. <i>Science Immunology</i>. AAAS. <a href=\"https://doi.org/10.1126/sciimmunol.abc3979\">https://doi.org/10.1126/sciimmunol.abc3979</a>","ama":"Salzer E, Zoghi S, Kiss MG, et al. The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity. <i>Science Immunology</i>. 2020;5(49). doi:<a href=\"https://doi.org/10.1126/sciimmunol.abc3979\">10.1126/sciimmunol.abc3979</a>"},"month":"07","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_processing_charge":"No","quality_controlled":"1","publication_status":"published","type":"journal_article","author":[{"last_name":"Salzer","first_name":"Elisabeth","full_name":"Salzer, Elisabeth"},{"full_name":"Zoghi, Samaneh","first_name":"Samaneh","last_name":"Zoghi"},{"full_name":"Kiss, Máté G.","first_name":"Máté G.","last_name":"Kiss"},{"full_name":"Kage, Frieda","first_name":"Frieda","last_name":"Kage"},{"last_name":"Rashkova","first_name":"Christina","full_name":"Rashkova, Christina"},{"last_name":"Stahnke","full_name":"Stahnke, Stephanie","first_name":"Stephanie"},{"last_name":"Haimel","first_name":"Matthias","full_name":"Haimel, Matthias"},{"last_name":"Platzer","full_name":"Platzer, René","first_name":"René"},{"first_name":"Michael","full_name":"Caldera, Michael","last_name":"Caldera"},{"last_name":"Ardy","full_name":"Ardy, Rico Chandra","first_name":"Rico Chandra"},{"full_name":"Hoeger, Birgit","first_name":"Birgit","last_name":"Hoeger"},{"last_name":"Block","first_name":"Jana","full_name":"Block, Jana"},{"last_name":"Medgyesi","full_name":"Medgyesi, David","first_name":"David"},{"last_name":"Sin","full_name":"Sin, Celine","first_name":"Celine"},{"last_name":"Shahkarami","full_name":"Shahkarami, Sepideh","first_name":"Sepideh"},{"last_name":"Kain","first_name":"Renate","full_name":"Kain, Renate"},{"last_name":"Ziaee","first_name":"Vahid","full_name":"Ziaee, Vahid"},{"full_name":"Hammerl, Peter","first_name":"Peter","last_name":"Hammerl"},{"last_name":"Bock","full_name":"Bock, Christoph","first_name":"Christoph"},{"last_name":"Menche","first_name":"Jörg","full_name":"Menche, Jörg"},{"first_name":"Loïc","full_name":"Dupré, Loïc","last_name":"Dupré"},{"last_name":"Huppa","first_name":"Johannes B.","full_name":"Huppa, Johannes B."},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","first_name":"Michael K"},{"full_name":"Lomakin, Alexis","first_name":"Alexis","last_name":"Lomakin"},{"last_name":"Rottner","full_name":"Rottner, Klemens","first_name":"Klemens"},{"last_name":"Binder","full_name":"Binder, Christoph J.","first_name":"Christoph J."},{"last_name":"Stradal","full_name":"Stradal, Theresia E.B.","first_name":"Theresia E.B."},{"full_name":"Rezaei, Nima","first_name":"Nima","last_name":"Rezaei"},{"last_name":"Boztug","full_name":"Boztug, Kaan","first_name":"Kaan"}],"OA_place":"repository","language":[{"iso":"eng"}],"issue":"49","isi":1,"status":"public","date_updated":"2026-04-03T09:25:04Z","date_created":"2020-07-19T22:00:58Z"},{"file":[{"date_created":"2020-12-02T09:13:23Z","relation":"main_file","date_updated":"2020-12-02T09:13:23Z","success":1,"creator":"dernst","file_name":"2020_EMBO_Montesinos.pdf","checksum":"43d2b36598708e6ab05c69074e191d57","content_type":"application/pdf","file_id":"8827","access_level":"open_access","file_size":3497156}],"date_created":"2020-07-21T09:08:38Z","date_updated":"2025-04-15T06:37:27Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"ddc":["580"],"issue":"17","isi":1,"publication_status":"published","type":"journal_article","author":[{"first_name":"Juan C","full_name":"Montesinos López, Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","last_name":"Montesinos López","orcid":"0000-0001-9179-6099"},{"last_name":"Abuzeineh","full_name":"Abuzeineh, A","first_name":"A"},{"last_name":"Kopf","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2187-6656","full_name":"Kopf, Aglaja","first_name":"Aglaja"},{"full_name":"Juanes Garcia, Alba","first_name":"Alba","orcid":"0000-0002-1009-9652","last_name":"Juanes Garcia","id":"40F05888-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ötvös, Krisztina","first_name":"Krisztina","orcid":"0000-0002-5503-4983","last_name":"Ötvös","id":"29B901B0-F248-11E8-B48F-1D18A9856A87"},{"first_name":"J","full_name":"Petrášek, J","last_name":"Petrášek"},{"first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179"},{"orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","first_name":"Eva","full_name":"Benková, Eva"}],"file_date_updated":"2020-12-02T09:13:23Z","quality_controlled":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"Yes (via OA deal)","citation":{"ama":"Montesinos López JC, Abuzeineh A, Kopf A, et al. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. 2020;39(17). doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>","apa":"Montesinos López, J. C., Abuzeineh, A., Kopf, A., Juanes Garcia, A., Ötvös, K., Petrášek, J., … Benková, E. (2020). Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>","ieee":"J. C. Montesinos López <i>et al.</i>, “Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage,” <i>The Embo Journal</i>, vol. 39, no. 17. Embo Press, 2020.","chicago":"Montesinos López, Juan C, A Abuzeineh, Aglaja Kopf, Alba Juanes Garcia, Krisztina Ötvös, J Petrášek, Michael K Sixt, and Eva Benková. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>. Embo Press, 2020. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>.","mla":"Montesinos López, Juan C., et al. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>, vol. 39, no. 17, e104238, Embo Press, 2020, doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>.","short":"J.C. Montesinos López, A. Abuzeineh, A. Kopf, A. Juanes Garcia, K. Ötvös, J. Petrášek, M.K. Sixt, E. Benková, The Embo Journal 39 (2020).","ista":"Montesinos López JC, Abuzeineh A, Kopf A, Juanes Garcia A, Ötvös K, Petrášek J, Sixt MK, Benková E. 2020. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. The Embo Journal. 39(17), e104238."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa_version":"Published Version","month":"09","publisher":"Embo Press","external_id":{"isi":["000548311800001"],"pmid":["32667089"]},"publication":"The Embo Journal","pmid":1,"day":"01","acknowledgement":"We thank Takashi Aoyama, David Alabadi, and Bert De Rybel for sharing material, Jiří Friml, Maciek Adamowski, and Katerina Schwarzerová for inspiring discussions, and Martine De Cock for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by the Bioimaging Facility (BIF), especially to Robert Hauschild; and the Life Science Facility (LSF). J.C.M. is the recipient of a EMBO Long‐Term Fellowship (ALTF number 710‐2016). This work was supported with MEYS CR, project no.CZ.02.1.01/0.0/0.0/16_019/0000738 to J.P., and by the Austrian Science Fund (FWF01_I1774S) to E.B.","fulldoi":"https://doi.org/10.15252/embj.2019104238","doi":"10.15252/embj.2019104238","year":"2020","project":[{"grant_number":"ALTF710-2016","_id":"253E54C8-B435-11E9-9278-68D0E5697425","name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants"},{"_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","grant_number":"I 1774-B16","call_identifier":"FWF"}],"volume":39,"abstract":[{"text":"Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re‐organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells.","lang":"eng"}],"intvolume":"        39","article_number":"e104238","scopus_import":"1","title":"Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage","article_type":"original","date_published":"2020-09-01T00:00:00Z","publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"corr_author":"1","oa":1,"department":[{"_id":"MiSi"},{"_id":"EvBe"}],"_id":"8142"},{"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"citation":{"mla":"Nicolai, Leo, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>, vol. 11, 5778, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>.","short":"L. Nicolai, K. Schiefelbein, S. Lipsky, A. Leunig, M. Hoffknecht, K. Pekayvaz, B. Raude, C. Marx, A. Ehrlich, J. Pircher, Z. Zhang, I. Saleh, A.-K. Marel, A. Löf, T. Petzold, M. Lorenz, K. Stark, R. Pick, G. Rosenberger, L. Weckbach, B. Uhl, S. Xia, C.A. Reichel, B. Walzog, C. Schulz, V. Zheden, M. Bender, R. Li, S. Massberg, F.R. Gärtner, Nature Communications 11 (2020).","ista":"Nicolai L, Schiefelbein K, Lipsky S, Leunig A, Hoffknecht M, Pekayvaz K, Raude B, Marx C, Ehrlich A, Pircher J, Zhang Z, Saleh I, Marel A-K, Löf A, Petzold T, Lorenz M, Stark K, Pick R, Rosenberger G, Weckbach L, Uhl B, Xia S, Reichel CA, Walzog B, Schulz C, Zheden V, Bender M, Li R, Massberg S, Gärtner FR. 2020. Vascular surveillance by haptotactic blood platelets in inflammation and infection. Nature Communications. 11, 5778.","apa":"Nicolai, L., Schiefelbein, K., Lipsky, S., Leunig, A., Hoffknecht, M., Pekayvaz, K., … Gärtner, F. R. (2020). Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>","ieee":"L. Nicolai <i>et al.</i>, “Vascular surveillance by haptotactic blood platelets in inflammation and infection,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","ama":"Nicolai L, Schiefelbein K, Lipsky S, et al. Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>","chicago":"Nicolai, Leo, Karin Schiefelbein, Silvia Lipsky, Alexander Leunig, Marie Hoffknecht, Kami Pekayvaz, Ben Raude, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>."},"month":"11","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_processing_charge":"No","file_date_updated":"2020-11-23T13:29:49Z","quality_controlled":"1","publication_status":"published","type":"journal_article","author":[{"first_name":"Leo","full_name":"Nicolai, Leo","last_name":"Nicolai"},{"full_name":"Schiefelbein, Karin","first_name":"Karin","last_name":"Schiefelbein"},{"last_name":"Lipsky","full_name":"Lipsky, Silvia","first_name":"Silvia"},{"first_name":"Alexander","full_name":"Leunig, Alexander","last_name":"Leunig"},{"first_name":"Marie","full_name":"Hoffknecht, Marie","last_name":"Hoffknecht"},{"full_name":"Pekayvaz, Kami","first_name":"Kami","last_name":"Pekayvaz"},{"last_name":"Raude","first_name":"Ben","full_name":"Raude, Ben"},{"full_name":"Marx, Charlotte","first_name":"Charlotte","last_name":"Marx"},{"last_name":"Ehrlich","full_name":"Ehrlich, Andreas","first_name":"Andreas"},{"first_name":"Joachim","full_name":"Pircher, Joachim","last_name":"Pircher"},{"first_name":"Zhe","full_name":"Zhang, Zhe","last_name":"Zhang"},{"full_name":"Saleh, Inas","first_name":"Inas","last_name":"Saleh"},{"first_name":"Anna-Kristina","full_name":"Marel, Anna-Kristina","last_name":"Marel"},{"last_name":"Löf","first_name":"Achim","full_name":"Löf, Achim"},{"full_name":"Petzold, Tobias","first_name":"Tobias","last_name":"Petzold"},{"full_name":"Lorenz, Michael","first_name":"Michael","last_name":"Lorenz"},{"last_name":"Stark","first_name":"Konstantin","full_name":"Stark, Konstantin"},{"last_name":"Pick","full_name":"Pick, Robert","first_name":"Robert"},{"last_name":"Rosenberger","first_name":"Gerhild","full_name":"Rosenberger, Gerhild"},{"first_name":"Ludwig","full_name":"Weckbach, Ludwig","last_name":"Weckbach"},{"last_name":"Uhl","full_name":"Uhl, Bernd","first_name":"Bernd"},{"last_name":"Xia","first_name":"Sheng","full_name":"Xia, Sheng"},{"full_name":"Reichel, Christoph Andreas","first_name":"Christoph Andreas","last_name":"Reichel"},{"full_name":"Walzog, Barbara","first_name":"Barbara","last_name":"Walzog"},{"full_name":"Schulz, Christian","first_name":"Christian","last_name":"Schulz"},{"first_name":"Vanessa","full_name":"Zheden, Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","last_name":"Zheden","orcid":"0000-0002-9438-4783"},{"first_name":"Markus","full_name":"Bender, Markus","last_name":"Bender"},{"last_name":"Li","first_name":"Rong","full_name":"Li, Rong"},{"last_name":"Massberg","full_name":"Massberg, Steffen","first_name":"Steffen"},{"first_name":"Florian R","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner"}],"language":[{"iso":"eng"}],"ddc":["570"],"related_material":{"link":[{"url":"https://doi.org/10.1038/s41467-022-31310-7","relation":"erratum"}]},"isi":1,"status":"public","has_accepted_license":"1","date_created":"2020-11-22T23:01:23Z","date_updated":"2026-04-02T11:48:21Z","file":[{"checksum":"485b7b6cf30198ba0ce126491a28f125","content_type":"application/pdf","date_created":"2020-11-23T13:29:49Z","date_updated":"2020-11-23T13:29:49Z","relation":"main_file","creator":"dernst","file_name":"2020_NatureComm_Nicolai.pdf","success":1,"file_size":7035340,"access_level":"open_access","file_id":"8798"}],"_id":"8787","publication_identifier":{"eissn":["2041-1723"]},"corr_author":"1","department":[{"_id":"MiSi"},{"_id":"EM-Fac"}],"oa":1,"article_type":"original","date_published":"2020-11-13T00:00:00Z","intvolume":"        11","article_number":"5778","scopus_import":"1","title":"Vascular surveillance by haptotactic blood platelets in inflammation and infection","project":[{"name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"747687"}],"volume":11,"abstract":[{"lang":"eng","text":"Breakdown of vascular barriers is a major complication of inflammatory diseases. Anucleate platelets form blood-clots during thrombosis, but also play a crucial role in inflammation. While spatio-temporal dynamics of clot formation are well characterized, the cell-biological mechanisms of platelet recruitment to inflammatory micro-environments remain incompletely understood. Here we identify Arp2/3-dependent lamellipodia formation as a prominent morphological feature of immune-responsive platelets. Platelets use lamellipodia to scan for fibrin(ogen) deposited on the inflamed vasculature and to directionally spread, to polarize and to govern haptotactic migration along gradients of the adhesive ligand. Platelet-specific abrogation of Arp2/3 interferes with haptotactic repositioning of platelets to microlesions, thus impairing vascular sealing and provoking inflammatory microbleeding. During infection, haptotaxis promotes capture of bacteria and prevents hematogenic dissemination, rendering platelets gate-keepers of the inflamed microvasculature. Consequently, these findings identify haptotaxis as a key effector function of immune-responsive platelets."}],"ec_funded":1,"acknowledgement":"We thank Sebastian Helmer, Nicole Blount, Christine Mann, and Beate Jantz for technical assistance; Hellen Ishikawa-Ankerhold for help and advice; Michael Sixt for critical\r\ndiscussions. This study was supported by the DFG SFB 914 (S.M. [B02 and Z01], K.Sch.\r\n[B02], B.W. [A02 and Z03], C.A.R. [B03], C.S. [A10], J.P. [Gerok position]), the DFG\r\nSFB 1123 (S.M. [B06]), the DFG FOR 2033 (S.M. and F.G.), the German Center for\r\nCardiovascular Research (DZHK) (Clinician Scientist Program [L.N.], MHA 1.4VD\r\n[S.M.], Postdoc Start-up Grant, 81×3600213 [F.G.]), FP7 program (project 260309,\r\nPRESTIGE [S.M.]), FöFoLe project 1015/1009 (L.N.), FöFoLe project 947 (F.G.), the\r\nFriedrich-Baur-Stiftung project 41/16 (F.G.), and LMUexcellence NFF (F.G.). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no.\r\n833440) (S.M.). F.G. received funding from the European Union’s Horizon 2020 research\r\nand innovation program under the Marie Skłodowska-Curie grant agreement no.\r\n747687.","day":"13","fulldoi":"https://doi.org/10.1038/s41467-020-19515-0","year":"2020","doi":"10.1038/s41467-020-19515-0","publisher":"Springer Nature","external_id":{"pmid":["33188196"],"isi":["000594648000014"]},"publication":"Nature Communications","pmid":1}]