[{"project":[{"name":"The impact of deleterious mutations on small populations","grant_number":"26293","_id":"34d33d68-11ca-11ed-8bc3-ec13763c0ca8"}],"citation":{"short":"K. Khudiakova, N.H. Barton, G. Arnqvist, BioRxiv (n.d.).","ista":"Khudiakova K, Barton NH, Arnqvist G. Sign epistasis extends the effects of balancing selection on genetic diversity. bioRxiv, <a href=\"https://doi.org/10.1101/2025.04.09.647826\">10.1101/2025.04.09.647826</a>.","ieee":"K. Khudiakova, N. H. Barton, and G. Arnqvist, “Sign epistasis extends the effects of balancing selection on genetic diversity,” <i>bioRxiv</i>. .","mla":"Khudiakova, Kseniia, et al. “Sign Epistasis Extends the Effects of Balancing Selection on Genetic Diversity.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.04.09.647826\">10.1101/2025.04.09.647826</a>.","apa":"Khudiakova, K., Barton, N. H., &#38; Arnqvist, G. (n.d.). Sign epistasis extends the effects of balancing selection on genetic diversity. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.04.09.647826\">https://doi.org/10.1101/2025.04.09.647826</a>","ama":"Khudiakova K, Barton NH, Arnqvist G. Sign epistasis extends the effects of balancing selection on genetic diversity. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.04.09.647826\">10.1101/2025.04.09.647826</a>","chicago":"Khudiakova, Kseniia, Nicholas H Barton, and Goran Arnqvist. “Sign Epistasis Extends the Effects of Balancing Selection on Genetic Diversity.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.04.09.647826\">https://doi.org/10.1101/2025.04.09.647826</a>."},"oa":1,"abstract":[{"text":"Balancing selection, a form of selection that maintains genetic diversity, is difficult to detect, and the importance of balancing selection for the maintenance of genetic variation may be larger than often assumed. We model the possibility that the diversity-promoting effects of balancing selection extend to other loci that show sign epistasis with a locus under balancing selection. Rather than focusing on overdominance, as was done in previous efforts, we explore the effects of negative frequency dependence and show that this has important effects on the conditions under which the diversity-promoting effect of epistasis can occur in diploids. Our results show that not only recombination rate but also the dominance of sign epistasis are key parameters that determine the maintenance of polymorphism beyond the locus under direct balancing selection. We suggest that the effect we explore may play a significant role, especially when balancing selection acts on major effect loci.","lang":"eng"}],"date_updated":"2026-06-12T12:43:34Z","main_file_link":[{"url":"https://doi.org/10.1101/2025.04.09.647826","open_access":"1"}],"corr_author":"1","article_processing_charge":"No","OA_place":"repository","title":"Sign epistasis extends the effects of balancing selection on genetic diversity","language":[{"iso":"eng"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","publication":"bioRxiv","date_published":"2026-04-23T00:00:00Z","day":"23","acknowledgement":"This work was funded by grants from the Swedish Research Council (2023-03730 to G.A.) and the DOC fellowship from the Austrian Academy of Science (26293 to K.K.).","OA_type":"green","_id":"21968","year":"2026","department":[{"_id":"NiBa"},{"_id":"JaMa"}],"date_created":"2026-06-09T12:26:11Z","publication_status":"draft","oa_version":"Preprint","related_material":{"record":[{"id":"21918","status":"public","relation":"dissertation_contains"}]},"author":[{"full_name":"Khudiakova, Kseniia","id":"4E6DC800-AE37-11E9-AC72-31CAE5697425","first_name":"Kseniia","orcid":"0000-0002-6246-1465","last_name":"Khudiakova"},{"last_name":"Barton","orcid":"0000-0002-8548-5240","first_name":"Nicholas H","id":"4880FE40-F248-11E8-B48F-1D18A9856A87","full_name":"Barton, Nicholas H"},{"first_name":"Goran","last_name":"Arnqvist","full_name":"Arnqvist, Goran"}],"type":"preprint","month":"04","doi":"10.1101/2025.04.09.647826","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)"},"status":"public"},{"title":"Context-dependent effects of livestock grazing on forest attributes and ecosystem multifunctionality in Nothofagus forests","supplementarymaterial":"yes","date_updated":"2026-07-27T11:01:30Z","citation":{"short":"P. Rodríguez, V. Cruz Alonso, S. Romano, G. Bustamante, R.M. Soler Schaller, Agriculture, Ecosystems and Environment 400 (2026).","ista":"Rodríguez P, Cruz Alonso V, Romano S, Bustamante G, Soler Schaller RM. 2026. Context-dependent effects of livestock grazing on forest attributes and ecosystem multifunctionality in Nothofagus forests. Agriculture, Ecosystems and Environment. 400, 110219.","mla":"Rodríguez, Paula, et al. “Context-Dependent Effects of Livestock Grazing on Forest Attributes and Ecosystem Multifunctionality in Nothofagus Forests.” <i>Agriculture, Ecosystems and Environment</i>, vol. 400, 110219, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.agee.2026.110219\">10.1016/j.agee.2026.110219</a>.","ieee":"P. Rodríguez, V. Cruz Alonso, S. Romano, G. Bustamante, and R. M. Soler Schaller, “Context-dependent effects of livestock grazing on forest attributes and ecosystem multifunctionality in Nothofagus forests,” <i>Agriculture, Ecosystems and Environment</i>, vol. 400. Elsevier, 2026.","apa":"Rodríguez, P., Cruz Alonso, V., Romano, S., Bustamante, G., &#38; Soler Schaller, R. M. (2026). Context-dependent effects of livestock grazing on forest attributes and ecosystem multifunctionality in Nothofagus forests. <i>Agriculture, Ecosystems and Environment</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.agee.2026.110219\">https://doi.org/10.1016/j.agee.2026.110219</a>","ama":"Rodríguez P, Cruz Alonso V, Romano S, Bustamante G, Soler Schaller RM. Context-dependent effects of livestock grazing on forest attributes and ecosystem multifunctionality in Nothofagus forests. <i>Agriculture, Ecosystems and Environment</i>. 2026;400. doi:<a href=\"https://doi.org/10.1016/j.agee.2026.110219\">10.1016/j.agee.2026.110219</a>","chicago":"Rodríguez, Paula, Verónica Cruz Alonso, Silvina Romano, Gimena Bustamante, and Rosina Matilde Soler Schaller. “Context-Dependent Effects of Livestock Grazing on Forest Attributes and Ecosystem Multifunctionality in Nothofagus Forests.” <i>Agriculture, Ecosystems and Environment</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.agee.2026.110219\">https://doi.org/10.1016/j.agee.2026.110219</a>."},"_id":"21036","scopus_import":"1","dataavailabilitystatement":"The authors do not have permission to share data.","acknowledgement":"We would like to thank Guillermo Ortiz (CADIC-CONICET) for his invaluable support in the field and lab work. We are extremely grateful to the ranchers for kindly allowing us access to their fields. Funding for this work was provided by the Argentine National Scientific and Technical Research Council (CONICET) and the National Agency for Scientific Promotion through project PICT 2019–675. PR was also granted the Mobility Scholarship Program 2025 between Andalusian and Ibero-American Universities (AUIP). VCA is co-supported by the Community of Madrid under the 2024 call for the ‘César Nombela’ research talent attraction programme (2024-T1/ECO-31335).","article_number":"110219","day":"15","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Rodríguez, Paula","first_name":"Paula","last_name":"Rodríguez"},{"last_name":"Cruz Alonso","first_name":"Verónica","full_name":"Cruz Alonso, Verónica"},{"first_name":"Silvina","last_name":"Romano","full_name":"Romano, Silvina"},{"first_name":"Gimena","last_name":"Bustamante","full_name":"Bustamante, Gimena"},{"full_name":"Soler Schaller, Rosina Matilde","last_name":"Soler Schaller","first_name":"Rosina Matilde","id":"9e668447-8c32-11ed-b0c7-8dc2d7b80803"}],"publication_status":"published","date_created":"2026-01-25T23:01:38Z","department":[{"_id":"NiBa"}],"status":"public","type":"journal_article","month":"04","language":[{"iso":"eng"}],"article_processing_charge":"No","publisher":"Elsevier","abstract":[{"text":"Forests under livestock grazing sustain important ecosystem services but face potential trade-offs between production and ecological integrity. While the effects of grazing on individual forest attributes are well documented, their integrated consequences remain poorly understood, particularly in temperate forest ecosystems. We evaluated the combined influence of livestock grazing intensity and canopy cover on individual attributes and ecosystem multifunctionality in native Nothofagus forests of Tierra del Fuego, Argentina. Across eight ranches spanning two agroecological regions (Ecotone and Mountain Range), we quantified forest regeneration, understorey richness and biomass, and soil properties, integrating them into a multifunctionality index. Using generalized linear mixed models, we found strong context-dependence: in the Mountain Range, higher grazing intensity reduced seedling and sapling density, organic matter content, coarse woody debris, and overall multifunctionality. In the Ecotone, these effects of livestock use intensity were attenuated, and canopy cover diminished sapling density and multifunctionality, but moderate cover enhanced understorey. Our results extend multifunctionality research from grazed grasslands to grazed temperate forests and show that ecological responses and trade-offs vary across landscape units. We conclude that the Mountain Range is more vulnerable to grazing, requiring stricter management, whereas the Ecotone retains greater capacity to sustain multifunctionality under controlled livestock use intensity. These findings underscore the importance of region-specific silvopastoral strategies that reconcile food production with forest conservation in southern Patagonia and comparable temperate forest landscapes worldwide.","lang":"eng"}],"das_tickbox":"1","OA_type":"closed access","intvolume":"       400","publication":"Agriculture, Ecosystems and Environment","date_published":"2026-04-15T00:00:00Z","volume":400,"article_type":"original","oa_version":"None","researchdata_availability":"no","year":"2026","quality_controlled":"1","doi":"10.1016/j.agee.2026.110219","publication_identifier":{"issn":["0167-8809"]}},{"das_tickbox":"1","publication":"Current Opinion in Genetics and Development","date_published":"2026-06-01T00:00:00Z","volume":98,"OA_type":"hybrid","intvolume":"        98","language":[{"iso":"eng"}],"article_processing_charge":"Yes (via OA deal)","abstract":[{"lang":"eng","text":"Promoters and enhancers are cis-regulatory elements (CREs), DNA sequences that bind transcription factor (TF) proteins to up- or down-regulate target genes. Decades-long efforts yielded TF-DNA interaction models that predict how strongly an individual TF binds arbitrary DNA sequences and how individual binding events on the CRE combine to affect gene expression. These insights can be synthesized into a global, biophysically realistic, and quantitative genotype-phenotype (GP) map for gene regulation, a ‘holy grail’ for the application of evolutionary theory. A global map provides a rare opportunity to simulate the long-term evolution of regulatory sequences and pose several fundamental questions: How long does it take to evolve CREs de novo? How many non-trivial regulatory functions exist in sequence space? How connected are they? For which regulatory architecture is CRE evolution most rapid and evolvable? In this article, the second of a two-part series, we review the application of evolutionary concepts — epistasis, robustness, evolvability, tunability, plasticity, and bet-hedging — to the evolution of gene regulatory sequences. We then evaluate the potential for a unifying theory for the evolution of regulatory sequences and identify key open challenges."}],"publisher":"Elsevier","quality_controlled":"1","doi":"10.1016/j.gde.2026.102472","has_accepted_license":"1","publication_identifier":{"eissn":["1879-0380"],"issn":["0959-437X"]},"oa_version":"Published Version","ddc":["570"],"article_type":"review","researchdata_availability":"no","year":"2026","scopus_import":"1","file_date_updated":"2026-07-27T13:39:59Z","_id":"21759","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","dataavailabilitystatement":"No data were used for the research described in the article.","acknowledgement":"We thank Calin Guet and Santiago Herrera-Álvarez for essential contributions to this manuscript.\r\nE.M. acknowledges support from the APART-USA fellowship, jointly funded by the Austrian Academy of Sciences (ÖAW) and the Institute of Science and Technology Austria (ISTA). N.B. acknowledges funding from the ERC Advanced Grant 101055327 “HaplotypeStructure”.\r\nThis study was also supported by the European Molecular Biology Laboratory (N.O.B., J.C.).","day":"01","article_number":"102472","corr_author":"1","supplementarymaterial":"no","title":"Long-term evolution of regulatory DNA sequences. Part 2: Theory and future challenges","OA_place":"publisher","oa":1,"citation":{"short":"E. Mascolo, R.E. Körei, N.O. Borst, N.H. Barton, J. Crocker, G. Tkačik, Current Opinion in Genetics and Development 98 (2026).","ista":"Mascolo E, Körei RE, Borst NO, Barton NH, Crocker J, Tkačik G. 2026. Long-term evolution of regulatory DNA sequences. Part 2: Theory and future challenges. Current Opinion in Genetics and Development. 98, 102472.","ieee":"E. Mascolo, R. E. Körei, N. O. Borst, N. H. Barton, J. Crocker, and G. Tkačik, “Long-term evolution of regulatory DNA sequences. Part 2: Theory and future challenges,” <i>Current Opinion in Genetics and Development</i>, vol. 98. Elsevier, 2026.","mla":"Mascolo, Elia, et al. “Long-Term Evolution of Regulatory DNA Sequences. Part 2: Theory and Future Challenges.” <i>Current Opinion in Genetics and Development</i>, vol. 98, 102472, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.gde.2026.102472\">10.1016/j.gde.2026.102472</a>.","apa":"Mascolo, E., Körei, R. E., Borst, N. O., Barton, N. H., Crocker, J., &#38; Tkačik, G. (2026). Long-term evolution of regulatory DNA sequences. Part 2: Theory and future challenges. <i>Current Opinion in Genetics and Development</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.gde.2026.102472\">https://doi.org/10.1016/j.gde.2026.102472</a>","chicago":"Mascolo, Elia, Reka E Körei, Noa O. Borst, Nicholas H Barton, Justin Crocker, and Gašper Tkačik. “Long-Term Evolution of Regulatory DNA Sequences. Part 2: Theory and Future Challenges.” <i>Current Opinion in Genetics and Development</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.gde.2026.102472\">https://doi.org/10.1016/j.gde.2026.102472</a>.","ama":"Mascolo E, Körei RE, Borst NO, Barton NH, Crocker J, Tkačik G. Long-term evolution of regulatory DNA sequences. Part 2: Theory and future challenges. <i>Current Opinion in Genetics and Development</i>. 2026;98. doi:<a href=\"https://doi.org/10.1016/j.gde.2026.102472\">10.1016/j.gde.2026.102472</a>"},"project":[{"_id":"bd6958e0-d553-11ed-ba76-86eba6a76c00","name":"Understanding the evolution of continuous genomes","grant_number":"101055327"}],"PlanS_conform":"1","date_updated":"2026-07-27T13:40:24Z","status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"file_name":"2026_CurrentOpinionGeneticsDev_Mascolo.pdf","date_created":"2026-07-27T13:39:59Z","date_updated":"2026-07-27T13:39:59Z","access_level":"open_access","relation":"main_file","file_size":3190001,"content_type":"application/pdf","checksum":"ac8bbee61717bfe7116e312cc6825259","file_id":"22590","success":1,"creator":"dernst"}],"type":"journal_article","month":"06","publication_status":"published","author":[{"id":"776a6ed0-a053-11f0-8635-80b95e0e0d53","last_name":"Mascolo","first_name":"Elia","orcid":"0000-0003-2977-7844","full_name":"Mascolo, Elia"},{"last_name":"Körei","first_name":"Reka E","id":"50FDE43E-AA30-11E9-A72B-8A12E6697425","full_name":"Körei, Reka E"},{"full_name":"Borst, Noa O.","last_name":"Borst","first_name":"Noa O."},{"full_name":"Barton, Nicholas H","last_name":"Barton","orcid":"0000-0002-8548-5240","first_name":"Nicholas H","id":"4880FE40-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Crocker, Justin","first_name":"Justin","last_name":"Crocker"},{"id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","last_name":"Tkačik","orcid":"0000-0002-6699-1455","first_name":"Gašper","full_name":"Tkačik, Gašper"}],"department":[{"_id":"GaTk"},{"_id":"NiBa"}],"date_created":"2026-04-26T22:01:46Z"},{"intvolume":"       231","OA_type":"hybrid","volume":231,"date_published":"2025-12-01T00:00:00Z","publication":"Genetics","publisher":"Oxford University Press","abstract":[{"lang":"eng","text":"Genetic variation that influences complex disease susceptibility is introduced into the population by mutation and removed by natural selection and genetic drift. This mutation–selection–drift balance (MSDB) shapes the prevalence of a disease and its genetic architecture. To date, however, MSDB has been modeled only for monogenic (Mendelian) diseases. Here, we develop an MSDB model for complex disease susceptibility: we assume that genotype relates to disease risk according to the canonical liability threshold model and that the selection on variants affecting risk stems from the fitness cost of the disease. We focus on diseases that are highly polygenic, entail a substantial fitness cost, and are neither extremely common in the population nor exceedingly rare. The comparison of model predictions with genome-wide association studies and other observations in humans indicates that common genetic variation affecting complex disease susceptibility is little affected by directional selection and instead shaped by pleiotropic stabilizing selection on other traits. In turn, directional selection may exert a more substantial effect on rare, large-effect variants. Our results also suggest that current estimates of disease heritability are likely biased. The model thus provides a better understanding of the evolutionary processes that shape the architecture and prevalence of complex diseases."}],"article_processing_charge":"Yes (in subscription journal)","language":[{"iso":"eng"}],"publication_identifier":{"issn":["0016-6731"],"eissn":["1943-2631"]},"has_accepted_license":"1","doi":"10.1093/genetics/iyaf220","quality_controlled":"1","year":"2025","article_type":"original","oa_version":"Published Version","ddc":["570"],"article_number":"iyaf220","day":"01","acknowledgement":"We thank Nick Barton, Magnus Nordborg, John Novembre, Molly Przeworski, and Himani Sachdeva for many helpful discussions and for comments on the manuscript, and we thank Joshua Schraiber and 2 anonymous reviewers for comments on the manuscript. We also thank members of the Sella, Przeworski and Andolfatto labs at Columbia University, and the Berg, Novembre and Steinrücken labs at the University of Chicago, for feedback on the work at various stages. This work was completed in part with resources provided by the University of Chicago's Research Computing Center. This work was supported by National Institutes of Health F32 grant GM126787 and R35 grant GM151257 to J.J.B. and National Institutes of Health R01 grant GM115889 to G.S.","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"4","_id":"20848","file_date_updated":"2025-12-29T11:27:51Z","scopus_import":"1","date_updated":"2025-12-29T11:29:16Z","citation":{"ama":"Berg JJ, Li X, Riall K, Hayward L, Sella G. Mutation–selection–drift balance models of complex diseases. <i>Genetics</i>. 2025;231(4). doi:<a href=\"https://doi.org/10.1093/genetics/iyaf220\">10.1093/genetics/iyaf220</a>","chicago":"Berg, Jeremy J., Xinyi Li, Kellen Riall, Laura Hayward, and Guy Sella. “Mutation–Selection–Drift Balance Models of Complex Diseases.” <i>Genetics</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/genetics/iyaf220\">https://doi.org/10.1093/genetics/iyaf220</a>.","apa":"Berg, J. J., Li, X., Riall, K., Hayward, L., &#38; Sella, G. (2025). Mutation–selection–drift balance models of complex diseases. <i>Genetics</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/genetics/iyaf220\">https://doi.org/10.1093/genetics/iyaf220</a>","mla":"Berg, Jeremy J., et al. “Mutation–Selection–Drift Balance Models of Complex Diseases.” <i>Genetics</i>, vol. 231, no. 4, iyaf220, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/genetics/iyaf220\">10.1093/genetics/iyaf220</a>.","ieee":"J. J. Berg, X. Li, K. Riall, L. Hayward, and G. Sella, “Mutation–selection–drift balance models of complex diseases,” <i>Genetics</i>, vol. 231, no. 4. Oxford University Press, 2025.","ista":"Berg JJ, Li X, Riall K, Hayward L, Sella G. 2025. Mutation–selection–drift balance models of complex diseases. Genetics. 231(4), iyaf220.","short":"J.J. Berg, X. Li, K. Riall, L. Hayward, G. Sella, Genetics 231 (2025)."},"oa":1,"OA_place":"publisher","title":"Mutation–selection–drift balance models of complex diseases","type":"journal_article","month":"12","file":[{"success":1,"checksum":"b02eb6b78028b8bef435edc8435a8468","file_id":"20863","content_type":"application/pdf","file_size":1182339,"creator":"dernst","relation":"main_file","access_level":"open_access","date_created":"2025-12-29T11:27:51Z","date_updated":"2025-12-29T11:27:51Z","file_name":"2025_Genetics_Berg.pdf"}],"tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"status":"public","date_created":"2025-12-21T23:01:34Z","external_id":{"pmid":["41073879"]},"department":[{"_id":"NiBa"}],"author":[{"full_name":"Berg, Jeremy J.","first_name":"Jeremy J.","last_name":"Berg"},{"full_name":"Li, Xinyi","last_name":"Li","first_name":"Xinyi"},{"last_name":"Riall","first_name":"Kellen","full_name":"Riall, Kellen"},{"full_name":"Hayward, Laura","id":"fc885ee5-24bf-11eb-ad7b-bcc5104c0c1b","first_name":"Laura","last_name":"Hayward"},{"last_name":"Sella","first_name":"Guy","full_name":"Sella, Guy"}],"pmid":1,"publication_status":"published"},{"publisher":"Wiley","abstract":[{"text":"Premise: What maintains trait divergence in the face of gene flow? Two varieties of wild snapdragon (Antirrhinum majus) characterized by divergent flower color hybridize in their native range. Selection on flower color genes is indicated by sharp clines, but the selective agents have not been demonstrated. Although previous work has focused on pollinators, pigmentation genes can also contribute to abiotic stress tolerance. We hypothesized that pigmentation in A. majus mediates stress tolerance, which could contribute to hybrid zone maintenance through parental niche divergence or hybrid maladaptation. Specifically, we tested whether morphotype mediates drought tolerance in an experiment comparing magenta-flowered var. pseudomajus, yellow-flowered var. striatum, and their pink-flowered hybrid cross.\r\nMethods: We experimentally compared drought tolerance of each morphotype from allopatric crosses within and between varieties using three greenhouse treatments. Control plants were watered as needed, while drought-treated plants were watered half as often, either from the transplant stage (“early” drought), or from flowering onset (“late” drought).\r\nResults: Parental morphotypes responded identically to drought in fitness and most phenotypic traits. However, hybrids had lower survival (14%) under late drought stress than parental morphotypes (70%). All hybrids that flowered in the late drought treatment died, compared to ~20% of flowering parental morphotypes.\r\nConclusions: Hybrid maladaptation to abiotic stress could potentially contribute to flower color divergence in the face of gene flow in A. majus. Further research should test the relevance of our results to field conditions and explicitly probe the role of flower color genes in drought tolerance.","lang":"eng"}],"article_processing_charge":"No","language":[{"iso":"eng"}],"intvolume":"       112","OA_type":"closed access","volume":112,"ec_funded":1,"publication":"American Journal of Botany","date_published":"2025-12-01T00:00:00Z","year":"2025","article_type":"original","oa_version":"None","publication_identifier":{"issn":["0002-9122"],"eissn":["1537-2197"]},"doi":"10.1002/ajb2.70129","quality_controlled":"1","date_updated":"2026-01-05T11:56:22Z","project":[{"_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships"}],"citation":{"apa":"Fuster‐Calvo, A., Jaworski, C. C., Ellis, T., &#38; Baskett, C. (2025). Reduced fitness under drought stress in F1 hybrids of Antirrhinum majus varieties with divergent flower colors. <i>American Journal of Botany</i>. Wiley. <a href=\"https://doi.org/10.1002/ajb2.70129\">https://doi.org/10.1002/ajb2.70129</a>","chicago":"Fuster‐Calvo, Alexandre, Coline C. Jaworski, Thomas Ellis, and Carina Baskett. “Reduced Fitness under Drought Stress in F1 Hybrids of Antirrhinum Majus Varieties with Divergent Flower Colors.” <i>American Journal of Botany</i>. Wiley, 2025. <a href=\"https://doi.org/10.1002/ajb2.70129\">https://doi.org/10.1002/ajb2.70129</a>.","ama":"Fuster‐Calvo A, Jaworski CC, Ellis T, Baskett C. Reduced fitness under drought stress in F1 hybrids of Antirrhinum majus varieties with divergent flower colors. <i>American Journal of Botany</i>. 2025;112(12). doi:<a href=\"https://doi.org/10.1002/ajb2.70129\">10.1002/ajb2.70129</a>","ista":"Fuster‐Calvo A, Jaworski CC, Ellis T, Baskett C. 2025. Reduced fitness under drought stress in F1 hybrids of Antirrhinum majus varieties with divergent flower colors. American Journal of Botany. 112(12), e70129.","short":"A. Fuster‐Calvo, C.C. Jaworski, T. Ellis, C. Baskett, American Journal of Botany 112 (2025).","ieee":"A. Fuster‐Calvo, C. C. Jaworski, T. Ellis, and C. Baskett, “Reduced fitness under drought stress in F1 hybrids of Antirrhinum majus varieties with divergent flower colors,” <i>American Journal of Botany</i>, vol. 112, no. 12. Wiley, 2025.","mla":"Fuster‐Calvo, Alexandre, et al. “Reduced Fitness under Drought Stress in F1 Hybrids of Antirrhinum Majus Varieties with Divergent Flower Colors.” <i>American Journal of Botany</i>, vol. 112, no. 12, e70129, Wiley, 2025, doi:<a href=\"https://doi.org/10.1002/ajb2.70129\">10.1002/ajb2.70129</a>."},"title":"Reduced fitness under drought stress in F1 hybrids of Antirrhinum majus varieties with divergent flower colors","article_number":"e70129","day":"01","acknowledgement":"Thank you to Doug Schemske and Nick Barton forcritically reviewing the manuscript, Beatriz Pablo Car-mona for assisting with data collection, Melinda Pickupand Eva Cereghetti for seedlings, and Louise Arathoon,Ksenia Khudiakova, Georg Rieckh, Daria Shiplina, andAnja Westram for help with experimental maintenance.We sincerely thank the Associate Editor Brenda Grewelland two anonymous reviewers for their thoughtfulcomments and suggestions, which substantially improved the clarity and quality of our manuscript. C.B. receivedfunding from the European Union's Horizon 2020 researchand innovation programme under the Marie Skłodowska‐Curie Grant Agreement No. 754411","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"12","_id":"20869","scopus_import":"1","date_created":"2025-12-29T12:14:26Z","external_id":{"pmid":["41327576 "]},"department":[{"_id":"NiBa"}],"author":[{"first_name":"Alexandre","last_name":"Fuster‐Calvo","full_name":"Fuster‐Calvo, Alexandre"},{"full_name":"Jaworski, Coline C.","first_name":"Coline C.","last_name":"Jaworski"},{"full_name":"Ellis, Thomas","id":"3153D6D4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8511-0254","first_name":"Thomas","last_name":"Ellis"},{"full_name":"Baskett, Carina","id":"3B4A7CE2-F248-11E8-B48F-1D18A9856A87","last_name":"Baskett","first_name":"Carina","orcid":"0000-0002-7354-8574"}],"pmid":1,"publication_status":"published","related_material":{"link":[{"url":"https://github.com/Alex-Fuster/hybrids_drought","relation":"software"}]},"type":"journal_article","month":"12","status":"public"},{"date_updated":"2026-04-07T08:45:14Z","oa":1,"citation":{"ieee":"O. O. Olusanya, K. Khudiakova, and H. Sachdeva, “Genetic load, eco-evolutionary feedback, and extinction in metapopulations,” <i>The American Naturalist</i>, vol. 205, no. 6. University of Chicago Press, pp. 617–636, 2025.","mla":"Olusanya, Oluwafunmilola O., et al. “Genetic Load, Eco-Evolutionary Feedback, and Extinction in Metapopulations.” <i>The American Naturalist</i>, vol. 205, no. 6, University of Chicago Press, 2025, pp. 617–36, doi:<a href=\"https://doi.org/10.1086/735562\">10.1086/735562</a>.","ista":"Olusanya OO, Khudiakova K, Sachdeva H. 2025. Genetic load, eco-evolutionary feedback, and extinction in metapopulations. The American Naturalist. 205(6), 617–636.","short":"O.O. Olusanya, K. Khudiakova, H. Sachdeva, The American Naturalist 205 (2025) 617–636.","ama":"Olusanya OO, Khudiakova K, Sachdeva H. Genetic load, eco-evolutionary feedback, and extinction in metapopulations. <i>The American Naturalist</i>. 2025;205(6):617-636. doi:<a href=\"https://doi.org/10.1086/735562\">10.1086/735562</a>","chicago":"Olusanya, Oluwafunmilola O, Kseniia Khudiakova, and Himani Sachdeva. “Genetic Load, Eco-Evolutionary Feedback, and Extinction in Metapopulations.” <i>The American Naturalist</i>. University of Chicago Press, 2025. <a href=\"https://doi.org/10.1086/735562\">https://doi.org/10.1086/735562</a>.","apa":"Olusanya, O. O., Khudiakova, K., &#38; Sachdeva, H. (2025). Genetic load, eco-evolutionary feedback, and extinction in metapopulations. <i>The American Naturalist</i>. University of Chicago Press. <a href=\"https://doi.org/10.1086/735562\">https://doi.org/10.1086/735562</a>"},"project":[{"grant_number":"P32896","name":"Causes and consequences of population fragmentation","_id":"c08d3278-5a5b-11eb-8a69-fdb09b55f4b8"},{"name":"Polygenic Adaptation in a Metapopulation","grant_number":"26380","_id":"34c872fe-11ca-11ed-8bc3-8534b82131e6"},{"_id":"34d33d68-11ca-11ed-8bc3-ec13763c0ca8","name":"The impact of deleterious mutations on small populations","grant_number":"26293"}],"title":"Genetic load, eco-evolutionary feedback, and extinction in metapopulations","OA_place":"repository","corr_author":"1","acknowledgement":"This research was partially funded by the Austrian Science Fund (FWF P-32896B) and DOC Fellowships of the Austrian Academy of Sciences: grants 26380 (O.O.) and 26293 (K.K.). We thank Nick Barton for useful comments on the chapter in O.O.’s thesis that led to this article.","day":"01","issue":"6","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","_id":"21322","scopus_import":"1","external_id":{"pmid":["40446297 "]},"date_created":"2026-02-18T10:47:18Z","department":[{"_id":"JaMa"},{"_id":"NiBa"}],"author":[{"id":"41AD96DC-F248-11E8-B48F-1D18A9856A87","first_name":"Oluwafunmilola O","orcid":"0000-0003-1971-8314","last_name":"Olusanya","full_name":"Olusanya, Oluwafunmilola O"},{"last_name":"Khudiakova","orcid":"0000-0002-6246-1465","first_name":"Kseniia","id":"4E6DC800-AE37-11E9-AC72-31CAE5697425","full_name":"Khudiakova, Kseniia"},{"first_name":"Himani","last_name":"Sachdeva","id":"42377A0A-F248-11E8-B48F-1D18A9856A87","full_name":"Sachdeva, Himani"}],"pmid":1,"related_material":{"record":[{"relation":"earlier_version","status":"public","id":"14732"}]},"publication_status":"published","page":"617-636","month":"06","type":"journal_article","status":"public","publisher":"University of Chicago Press","main_file_link":[{"url":"https://doi.org/10.1101/2023.12.02.569702","open_access":"1"}],"abstract":[{"text":"Habitat fragmentation poses a significant risk to population survival, causing both demographic stochasticity and genetic drift within local populations to increase, thereby increasing genetic load. Higher load causes population numbers to decline, which reduces the efficiency of selection and further increases load, resulting in a positive feedback that may drive entire populations to extinction. Here, we investigate this eco-evolutionary feedback in a metapopulation consisting of local demes connected via migration, with individuals subject to deleterious mutation at a large number of loci. We first analyze the determinants of load under soft selection, where population sizes are fixed, and then build on this to understand hard selection, where population sizes and load coevolve. We show that under soft selection, very little gene flow (less than one migrant per generation) is enough to prevent fixation of deleterious alleles. By contrast, much higher levels of migration are required to mitigate load and prevent extinction when selection is hard, with critical migration thresholds for metapopulation persistence increasing sharply as the genome-wide deleterious mutation rate becomes comparable to the baseline population growth rate. Moreover, critical migration thresholds are highest if deleterious mutations have intermediate selection coefficients but lower if alleles are predominantly recessive rather than additive (due to more efficient purging of recessive load within local populations). Our analysis is based on a combination of analytical approximations and simulations, allowing for a more comprehensive understanding of the factors influencing load and extinction in fragmented populations.","lang":"eng"}],"language":[{"iso":"eng"}],"article_processing_charge":"No","OA_type":"green","intvolume":"       205","date_published":"2025-06-01T00:00:00Z","publication":"The American Naturalist","volume":205,"year":"2025","article_type":"original","oa_version":"Preprint","publication_identifier":{"issn":["0003-0147"],"eissn":["1537-5323"]},"quality_controlled":"1","doi":"10.1086/735562"},{"date_published":"2025-01-07T00:00:00Z","user_id":"9947682f-b9fa-11ee-9c4a-b3ffaafe6614","day":"07","file_date_updated":"2025-01-02T12:30:39Z","_id":"18712","abstract":[{"text":"This file contains the code associated with the manuscript 'Effect of assortative mating and sexual selection on polygenic barriers to gene flow'. ","lang":"eng"}],"oa":1,"citation":{"ama":"Surendranadh P, Sachdeva H. Mathematica notebook and Fortran code for “Effect of assortative mating and sexual selection on polygenic barriers to gene flow.” 2025. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:17344\">10.15479/AT:ISTA:17344</a>","chicago":"Surendranadh, Parvathy, and Himani Sachdeva. “Mathematica Notebook and Fortran Code for ‘Effect of Assortative Mating and Sexual Selection on Polygenic Barriers to Gene Flow.’” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT:ISTA:17344\">https://doi.org/10.15479/AT:ISTA:17344</a>.","apa":"Surendranadh, P., &#38; Sachdeva, H. (2025). Mathematica notebook and Fortran code for “Effect of assortative mating and sexual selection on polygenic barriers to gene flow.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:17344\">https://doi.org/10.15479/AT:ISTA:17344</a>","ieee":"P. Surendranadh and H. Sachdeva, “Mathematica notebook and Fortran code for ‘Effect of assortative mating and sexual selection on polygenic barriers to gene flow.’” Institute of Science and Technology Austria, 2025.","mla":"Surendranadh, Parvathy, and Himani Sachdeva. <i>Mathematica Notebook and Fortran Code for “Effect of Assortative Mating and Sexual Selection on Polygenic Barriers to Gene Flow.”</i> Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:17344\">10.15479/AT:ISTA:17344</a>.","ista":"Surendranadh P, Sachdeva H. 2025. Mathematica notebook and Fortran code for ‘Effect of assortative mating and sexual selection on polygenic barriers to gene flow’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:17344\">10.15479/AT:ISTA:17344</a>.","short":"P. Surendranadh, H. Sachdeva, (2025)."},"publisher":"Institute of Science and Technology Austria","date_updated":"2025-12-30T08:44:12Z","corr_author":"1","title":"Mathematica notebook and Fortran code for 'Effect of assortative mating and sexual selection on polygenic barriers to gene flow'","article_processing_charge":"No","file":[{"creator":"psurendr","checksum":"9c5f91876014706990a0728c3675cd2a","file_id":"18722","success":1,"content_type":"application/zip","file_size":326835,"relation":"main_file","access_level":"open_access","date_created":"2025-01-02T12:30:27Z","date_updated":"2025-01-02T12:30:27Z","file_name":"Codes.zip"},{"creator":"psurendr","file_size":620,"content_type":"text/plain","success":1,"file_id":"18723","checksum":"47fe98b7cc526e634e42de58f5eae288","file_name":"ReadMe.txt","date_created":"2025-01-02T12:30:39Z","date_updated":"2025-01-02T12:30:39Z","access_level":"open_access","relation":"main_file"}],"type":"research_data","month":"01","acknowledged_ssus":[{"_id":"ScienComp"}],"doi":"10.15479/AT:ISTA:17344","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"status":"public","has_accepted_license":"1","department":[{"_id":"GradSch"},{"_id":"NiBa"}],"year":"2025","date_created":"2025-01-01T15:28:27Z","oa_version":"Published Version","ddc":["576"],"related_material":{"record":[{"id":"19876","status":"public","relation":"used_in_publication"}]},"author":[{"last_name":"Surendranadh","first_name":"Parvathy","orcid":"0000-0001-6395-386X","id":"455235B8-F248-11E8-B48F-1D18A9856A87","full_name":"Surendranadh, Parvathy"},{"first_name":"Himani","last_name":"Sachdeva","full_name":"Sachdeva, Himani"}]},{"year":"2025","ddc":["570"],"oa_version":"Published Version","article_type":"original","publication_identifier":{"issn":["1010-061X"],"eissn":["1420-9101"]},"quality_controlled":"1","doi":"10.1093/jeb/voaf002","has_accepted_license":"1","abstract":[{"lang":"eng","text":"Polymorphic short insertions and deletions (INDELs \r\n 50 bp) are abundant, although less common than single nucleotide polymorphisms (SNPs). Evidence from model organisms shows INDELs to be more strongly influenced by purifying selection than SNPs. Partly for this reason, INDELs are rarely used as markers for demographic processes or to detect divergent selection. Here, we compared INDELs and SNPs in the intertidal snail Littorina saxatilis, focussing on hybrid zones between ecotypes, in order to test the utility of INDELs in the detection of divergent selection. We computed INDEL and SNP site frequency spectra using capture sequencing data. We assessed the impact of divergent selection by analyzing allele frequency clines across habitat boundaries. We also examined the influence of GC-biased gene conversion because it may be confounded with signatures of selection. We show evidence that short INDELs are affected more by purifying selection than SNPs, but part of the observed site frequency spectra difference can be attributed to GC-biased gene conversion. We did not find a difference in the impact of divergent selection between short INDELs and SNPs. Short INDELs and SNPs were similarly distributed across the genome and so are likely to respond to indirect selection in the same way. A few regions likely affected by divergent selection were revealed by INDELs and not by SNPs. Short INDELs can be useful (additional) genetic markers helping to identify genomic regions important for adaptation and population divergence."}],"publisher":"Oxford University Press","language":[{"iso":"eng"}],"article_processing_charge":"Yes (in subscription journal)","date_published":"2025-03-01T00:00:00Z","publication":"Journal of Evolutionary Biology","volume":38,"OA_type":"hybrid","intvolume":"        38","department":[{"_id":"NiBa"}],"date_created":"2025-03-23T23:01:25Z","external_id":{"pmid":["39803902"],"isi":["001415267900001"]},"publication_status":"published","pmid":1,"author":[{"last_name":"Perini","first_name":"Samuel","full_name":"Perini, Samuel"},{"full_name":"Johannesson, Kerstin","first_name":"Kerstin","last_name":"Johannesson"},{"last_name":"Butlin","first_name":"Roger K.","full_name":"Butlin, Roger K."},{"full_name":"Westram, Anja M","id":"3C147470-F248-11E8-B48F-1D18A9856A87","last_name":"Westram","orcid":"0000-0003-1050-4969","first_name":"Anja M"}],"page":"367-378","file":[{"creator":"dernst","checksum":"01408e626a4131bfec5ffc70b0af9129","file_id":"19469","success":1,"content_type":"application/pdf","file_size":12826085,"relation":"main_file","access_level":"open_access","date_created":"2025-04-03T11:53:06Z","date_updated":"2025-04-03T11:53:06Z","file_name":"2025_JourEvolBiology_Perini.pdf"}],"type":"journal_article","month":"03","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)"},"citation":{"apa":"Perini, S., Johannesson, K., Butlin, R. K., &#38; Westram, A. M. (2025). Short INDELs and SNPs as markers of evolutionary processes in hybrid zones. <i>Journal of Evolutionary Biology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jeb/voaf002\">https://doi.org/10.1093/jeb/voaf002</a>","ama":"Perini S, Johannesson K, Butlin RK, Westram AM. Short INDELs and SNPs as markers of evolutionary processes in hybrid zones. <i>Journal of Evolutionary Biology</i>. 2025;38(3):367-378. doi:<a href=\"https://doi.org/10.1093/jeb/voaf002\">10.1093/jeb/voaf002</a>","chicago":"Perini, Samuel, Kerstin Johannesson, Roger K. Butlin, and Anja M Westram. “Short INDELs and SNPs as Markers of Evolutionary Processes in Hybrid Zones.” <i>Journal of Evolutionary Biology</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/jeb/voaf002\">https://doi.org/10.1093/jeb/voaf002</a>.","short":"S. Perini, K. Johannesson, R.K. Butlin, A.M. Westram, Journal of Evolutionary Biology 38 (2025) 367–378.","ista":"Perini S, Johannesson K, Butlin RK, Westram AM. 2025. Short INDELs and SNPs as markers of evolutionary processes in hybrid zones. Journal of Evolutionary Biology. 38(3), 367–378.","mla":"Perini, Samuel, et al. “Short INDELs and SNPs as Markers of Evolutionary Processes in Hybrid Zones.” <i>Journal of Evolutionary Biology</i>, vol. 38, no. 3, Oxford University Press, 2025, pp. 367–78, doi:<a href=\"https://doi.org/10.1093/jeb/voaf002\">10.1093/jeb/voaf002</a>.","ieee":"S. Perini, K. Johannesson, R. K. Butlin, and A. M. Westram, “Short INDELs and SNPs as markers of evolutionary processes in hybrid zones,” <i>Journal of Evolutionary Biology</i>, vol. 38, no. 3. Oxford University Press, pp. 367–378, 2025."},"oa":1,"date_updated":"2025-09-30T11:19:56Z","corr_author":"1","isi":1,"title":"Short INDELs and SNPs as markers of evolutionary processes in hybrid zones","OA_place":"publisher","issue":"3","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","acknowledgement":"This work was supported by the Natural Environment Research Council (NE/K014021/1), European Research Council (ERC-2015-AdG-693030- BARRIERS) and Swedish Research Council VR (2018-03695) and we are also very grateful for the support of the Linnaeus Centre for Marine Evolutionary Biology at the University of Gothenburg.\r\nWe thank the Swedish Bioinformatics Advisory Program organized by SciLifeLab for feedback and assistance on the variant calling pipeline and Alan Le Moan for helpful discussions. R.K.B. and A.M.W. contributed equally to this work. We are also very grateful to Tomas Larsson and Marina Panova for their bioinformatic analyses on the genome and the annotation. The bioinformatic analyses were performed on resources at the University of Sheffield’s High Performance Computing cluster, ShARC. We thank two anonymous reviewers for helpful comments on a previous version.","day":"01","scopus_import":"1","file_date_updated":"2025-04-03T11:53:06Z","_id":"19438"},{"external_id":{"isi":["001443422900001"]},"date_created":"2025-03-23T23:01:27Z","department":[{"_id":"NiBa"}],"author":[{"last_name":"Heim","first_name":"Ramona Julia","full_name":"Heim, Ramona Julia"},{"full_name":"Rocha, Adrian V.","last_name":"Rocha","first_name":"Adrian V."},{"full_name":"Zemlianskii, Vitalii","last_name":"Zemlianskii","first_name":"Vitalii"},{"last_name":"Barrett","first_name":"Kirsten","full_name":"Barrett, Kirsten"},{"full_name":"Bültmann, Helga","first_name":"Helga","last_name":"Bültmann"},{"last_name":"Breen","first_name":"Amy","full_name":"Breen, Amy"},{"full_name":"Frost, Gerald Verner","first_name":"Gerald Verner","last_name":"Frost"},{"last_name":"Hollingsworth","first_name":"Teresa Nettleton","full_name":"Hollingsworth, Teresa Nettleton"},{"full_name":"Jandt, Randi","last_name":"Jandt","first_name":"Randi"},{"full_name":"Kozlova, Maria","first_name":"Maria","last_name":"Kozlova"},{"last_name":"Kurka","first_name":"Anastasiya","full_name":"Kurka, Anastasiya"},{"full_name":"Jorgenson, Mark Torre","last_name":"Jorgenson","first_name":"Mark Torre"},{"first_name":"Simon M.","last_name":"Landhäusser","full_name":"Landhäusser, Simon M."},{"full_name":"Loranty, Michael Mark","last_name":"Loranty","first_name":"Michael Mark"},{"last_name":"Miller","first_name":"Eric A.","full_name":"Miller, Eric A."},{"full_name":"Narita, Kenji","last_name":"Narita","first_name":"Kenji"},{"id":"0b30719b-13f0-11ed-ab2a-94498bc6a278","last_name":"Pravdolyubova","first_name":"Evgeniya","full_name":"Pravdolyubova, Evgeniya"},{"last_name":"Hölzel","first_name":"Norbert","full_name":"Hölzel, Norbert"},{"full_name":"Schaepman-Strub, Gabriela","first_name":"Gabriela","last_name":"Schaepman-Strub"}],"publication_status":"published","file":[{"relation":"main_file","access_level":"open_access","date_updated":"2025-12-30T08:08:18Z","date_created":"2025-12-30T08:08:18Z","file_name":"2025_JournEcology_Heim.pdf","checksum":"e2785ae265e211b4dc7fc9c5b7744948","file_id":"20890","success":1,"content_type":"application/pdf","file_size":2662766,"creator":"dernst"}],"page":"1042-1056","type":"journal_article","month":"05","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"status":"public","PlanS_conform":"1","date_updated":"2025-12-30T08:09:47Z","oa":1,"citation":{"mla":"Heim, Ramona Julia, et al. “Arctic Tundra Ecosystems under Fire—Alternative Ecosystem States in a Changing Climate?” <i>Journal of Ecology</i>, vol. 113, no. 5, Wiley, 2025, pp. 1042–56, doi:<a href=\"https://doi.org/10.1111/1365-2745.70022\">10.1111/1365-2745.70022</a>.","ieee":"R. J. Heim <i>et al.</i>, “Arctic tundra ecosystems under fire—Alternative ecosystem states in a changing climate?,” <i>Journal of Ecology</i>, vol. 113, no. 5. Wiley, pp. 1042–1056, 2025.","ista":"Heim RJ, Rocha AV, Zemlianskii V, Barrett K, Bültmann H, Breen A, Frost GV, Hollingsworth TN, Jandt R, Kozlova M, Kurka A, Jorgenson MT, Landhäusser SM, Loranty MM, Miller EA, Narita K, Pravdolyubova E, Hölzel N, Schaepman-Strub G. 2025. Arctic tundra ecosystems under fire—Alternative ecosystem states in a changing climate? Journal of Ecology. 113(5), 1042–1056.","short":"R.J. Heim, A.V. Rocha, V. Zemlianskii, K. Barrett, H. Bültmann, A. Breen, G.V. Frost, T.N. Hollingsworth, R. Jandt, M. Kozlova, A. Kurka, M.T. Jorgenson, S.M. Landhäusser, M.M. Loranty, E.A. Miller, K. Narita, E. Pravdolyubova, N. Hölzel, G. Schaepman-Strub, Journal of Ecology 113 (2025) 1042–1056.","ama":"Heim RJ, Rocha AV, Zemlianskii V, et al. Arctic tundra ecosystems under fire—Alternative ecosystem states in a changing climate? <i>Journal of Ecology</i>. 2025;113(5):1042-1056. doi:<a href=\"https://doi.org/10.1111/1365-2745.70022\">10.1111/1365-2745.70022</a>","chicago":"Heim, Ramona Julia, Adrian V. Rocha, Vitalii Zemlianskii, Kirsten Barrett, Helga Bültmann, Amy Breen, Gerald Verner Frost, et al. “Arctic Tundra Ecosystems under Fire—Alternative Ecosystem States in a Changing Climate?” <i>Journal of Ecology</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/1365-2745.70022\">https://doi.org/10.1111/1365-2745.70022</a>.","apa":"Heim, R. J., Rocha, A. V., Zemlianskii, V., Barrett, K., Bültmann, H., Breen, A., … Schaepman-Strub, G. (2025). Arctic tundra ecosystems under fire—Alternative ecosystem states in a changing climate? <i>Journal of Ecology</i>. Wiley. <a href=\"https://doi.org/10.1111/1365-2745.70022\">https://doi.org/10.1111/1365-2745.70022</a>"},"title":"Arctic tundra ecosystems under fire—Alternative ecosystem states in a changing climate?","OA_place":"publisher","isi":1,"acknowledgement":"We would like to express our sincere gratitude to all the data providers who carried out fieldwork in different regions of the Arctic and published their data, which we used for our meta-analysis. We recognise the hard work and dedication of these individuals, without whom this paper would not have been possible. We are grateful to the editor and the anonymous reviewer for their time and valuable feedback on this manuscript. We particularly appreciate the detailed and constructive comments provided by reviewer Mara Baudena, which significantly strengthened our work. We also acknowledge the Indigenous peoples and rural communities of the Arctic, whose traditional knowledge, rights, and interests are integral to the stewardship and study of these ecosystems. This work was funded in part by the U.S. National Aeronautics and Space Administration (NASA) grant 80NSSC22K1256 (GVF). Open Access funding enabled and organized by Projekt DEAL.","day":"01","issue":"5","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"19442","file_date_updated":"2025-12-30T08:08:18Z","scopus_import":"1","year":"2025","article_type":"review","oa_version":"Published Version","ddc":["550","570"],"publication_identifier":{"issn":["0022-0477"],"eissn":["1365-2745"]},"has_accepted_license":"1","quality_controlled":"1","doi":"10.1111/1365-2745.70022","publisher":"Wiley","abstract":[{"text":"1. Climate change is expected to induce shifts in the composition, structure and functioning of Arctic tundra ecosystems. Increases in the frequency and severity of tundra fires have the potential to catalyse vegetation transitions with far-reaching local, regional and global consequences.\r\n2. We propose that post-fire tundra recovery, coupled with climate change, may not necessarily lead to pre-fire conditions. Our hypothesis, based on surveys and literature, suggests two climate–fire driven trajectories. One trajectory results in increased woody vegetation under low fire frequency; the other results in grass dominance under high frequency.\r\n3. Future research should address uncertainties regarding possible tundra ecosystem shifts linked to fires, using methods that encompass greater temporal and spatial scales than previously addressed. More case studies, especially in underrepresented regions and ecosystem types, are essential to broaden the empirical basis for forecasts and potential fire management strategies.\r\n4. Synthesis. Our review synthesises current knowledge on post-fire vegetation trajectories in Arctic tundra ecosystems, highlighting potential transitions and alternative ecosystem states and their implications. We discuss challenges in defining and predicting these trajectories as well as future directions.","lang":"eng"}],"language":[{"iso":"eng"}],"article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","intvolume":"       113","publication":"Journal of Ecology","date_published":"2025-05-01T00:00:00Z","volume":113},{"article_processing_charge":"No","language":[{"iso":"eng"}],"abstract":[{"text":"Mycorrhizal and saprotrophic macromycetes contribute strongly to the carbon and nitrogen cycles of forest ecosystems, often studied by tracing stable isotope composition of carbon and nitrogen. The phenomenon of the saprotrophic-mycorrhizal divide highlights the difference in the stable isotope composition of fruiting bodies of mycorrhizal and saprotrophic fungi. Much less is known about the isotopic composition of the mycelium, which plays an important role in the formation of the soil organic matter and fuels the fungal trophic channel in soil food webs. In this study, we assessed whether the saprotrophic-mycorrhizal divide in the natural δ13С and δ15N values can be traced throughout entire fungal organisms. This hypothesis was tested using 16 species of ectomycorrhizal and six species of saprotrophic basidiomycetous fungi. We showed that not only fruiting bodies, but also the mycelium of ectomycorrhizal and saprotrophic fungi differs in the δ13C and δ15N values. In both ectomycorrhizal and saprotrophic fungi, the δ13C and δ15N values increased from mycelium to hymenophores and correlated positively with the total N content in the corresponding tissues. The differences between ectomycorrhizal and saprotrophic mycelium can be used to reconstruct the fungal-driven belowground carbon and nitrogen allocation, and the contribution of saprotrophic and mycorrhizal fungi to soil food webs.","lang":"eng"}],"publisher":"Springer Nature","volume":35,"publication":"Mycorrhiza","date_published":"2025-04-01T00:00:00Z","intvolume":"        35","OA_type":"closed access","oa_version":"None","article_type":"original","year":"2025","doi":"10.1007/s00572-025-01203-w","quality_controlled":"1","publication_identifier":{"eissn":["1432-1890"],"issn":["0940-6360"]},"isi":1,"title":"Saprotrophic-mycorrhizal divide in stable isotope composition throughout the whole fungus: From mycelium to hymenophore","citation":{"apa":"Zuev, A. G., Alexandrova, A. V., Litvinskiy, V. A., Pravdolyubova, E., &#38; Tiunov, A. V. (2025). Saprotrophic-mycorrhizal divide in stable isotope composition throughout the whole fungus: From mycelium to hymenophore. <i>Mycorrhiza</i>. Springer Nature. <a href=\"https://doi.org/10.1007/s00572-025-01203-w\">https://doi.org/10.1007/s00572-025-01203-w</a>","chicago":"Zuev, A. G., A. V. Alexandrova, V. A. Litvinskiy, Evgeniya Pravdolyubova, and A. V. Tiunov. “Saprotrophic-Mycorrhizal Divide in Stable Isotope Composition throughout the Whole Fungus: From Mycelium to Hymenophore.” <i>Mycorrhiza</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1007/s00572-025-01203-w\">https://doi.org/10.1007/s00572-025-01203-w</a>.","ama":"Zuev AG, Alexandrova AV, Litvinskiy VA, Pravdolyubova E, Tiunov AV. Saprotrophic-mycorrhizal divide in stable isotope composition throughout the whole fungus: From mycelium to hymenophore. <i>Mycorrhiza</i>. 2025;35(2). doi:<a href=\"https://doi.org/10.1007/s00572-025-01203-w\">10.1007/s00572-025-01203-w</a>","ista":"Zuev AG, Alexandrova AV, Litvinskiy VA, Pravdolyubova E, Tiunov AV. 2025. Saprotrophic-mycorrhizal divide in stable isotope composition throughout the whole fungus: From mycelium to hymenophore. Mycorrhiza. 35(2), 32.","short":"A.G. Zuev, A.V. Alexandrova, V.A. Litvinskiy, E. Pravdolyubova, A.V. Tiunov, Mycorrhiza 35 (2025).","mla":"Zuev, A. G., et al. “Saprotrophic-Mycorrhizal Divide in Stable Isotope Composition throughout the Whole Fungus: From Mycelium to Hymenophore.” <i>Mycorrhiza</i>, vol. 35, no. 2, 32, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1007/s00572-025-01203-w\">10.1007/s00572-025-01203-w</a>.","ieee":"A. G. Zuev, A. V. Alexandrova, V. A. Litvinskiy, E. Pravdolyubova, and A. V. Tiunov, “Saprotrophic-mycorrhizal divide in stable isotope composition throughout the whole fungus: From mycelium to hymenophore,” <i>Mycorrhiza</i>, vol. 35, no. 2. Springer Nature, 2025."},"date_updated":"2025-09-30T12:24:12Z","scopus_import":"1","_id":"19641","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"2","day":"01","article_number":"32","acknowledgement":"We thank Sergey Tsurikov for the help with stable isotope analysis. Dr. Jacob D. Wickham (IEE RAS) kindly improved the English of the manuscript. This work was supported by the Russian Science Foundation (project №. 22–14–00363).","publication_status":"published","author":[{"last_name":"Zuev","first_name":"A. G.","full_name":"Zuev, A. G."},{"full_name":"Alexandrova, A. V.","last_name":"Alexandrova","first_name":"A. V."},{"full_name":"Litvinskiy, V. A.","first_name":"V. A.","last_name":"Litvinskiy"},{"id":"0b30719b-13f0-11ed-ab2a-94498bc6a278","first_name":"Evgeniya","last_name":"Pravdolyubova","full_name":"Pravdolyubova, Evgeniya"},{"full_name":"Tiunov, A. V.","last_name":"Tiunov","first_name":"A. V."}],"pmid":1,"department":[{"_id":"NiBa"}],"date_created":"2025-05-04T22:02:32Z","external_id":{"isi":["001467249900001"],"pmid":["40232310"]},"status":"public","month":"04","type":"journal_article"},{"article_processing_charge":"No","language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"Silvopastoral use in native forests could impact population dynamics of key tree species, with contrasting effects at different life cycle stages. Prior studies in South American temperate forests have mainly focused on initial stages, lacking a comprehensive understanding of the entire life cycle within productive systems. We assessed the population dynamics of two key species of mixed forests in northern Patagonia (Austrocedrus chilensis and Nothofagus dombeyi) under two silvopastoral use intensities (high vs. low), using demographic techniques and population projection models. Over 3 years, we quantified vital rates (survival, fertility, growth, reversion and stasis) and used matrix models to calculate deterministic population growth rates (λ). High-intensity silvopastoral use had predominantly negative effects on the elements of the projection matrices of A. chilensis, whereas N. dombeyi exhibited mostly positive or no changes. As a result, projections indicated slight population decreases for A. chilensis (mostly λ < 1) at high silvopastoral use levels compared to low levels, while N. dombeyi showed similar projections (λ ≅ 1) between use levels. Decreased λ for A. chilensis resulted mainly from lower adult tree survival, while early life stages had limited influence on λ for these long-lived species. In summary, silvopastoral use affects population dynamics of key tree species of these mixed forests of northern Patagonia, with implications for sustainable management. Our findings highlight the importance of considering the entire life cycle and suggest targeted practices to enhance A. chilensis populations."}],"publisher":"Wiley","volume":50,"publication":"Austral Ecology","date_published":"2025-04-01T00:00:00Z","intvolume":"        50","OA_type":"closed access","oa_version":"None","article_type":"original","year":"2025","doi":"10.1111/aec.70058","quality_controlled":"1","publication_identifier":{"eissn":["1442-9993"],"issn":["1442-9985"]},"isi":1,"title":"A life-cycle approach to understand consequences of silvopastoral use on two native tree species of Northern Patagonia","citation":{"ista":"Arpigiani D, Aschero V, Soler Schaller RM, Amoroso MM. 2025. A life-cycle approach to understand consequences of silvopastoral use on two native tree species of Northern Patagonia. Austral Ecology. 50(4), e70058.","short":"D. Arpigiani, V. Aschero, R.M. Soler Schaller, M.M. Amoroso, Austral Ecology 50 (2025).","ieee":"D. Arpigiani, V. Aschero, R. M. Soler Schaller, and M. M. Amoroso, “A life-cycle approach to understand consequences of silvopastoral use on two native tree species of Northern Patagonia,” <i>Austral Ecology</i>, vol. 50, no. 4. Wiley, 2025.","mla":"Arpigiani, Daniela, et al. “A Life-Cycle Approach to Understand Consequences of Silvopastoral Use on Two Native Tree Species of Northern Patagonia.” <i>Austral Ecology</i>, vol. 50, no. 4, e70058, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/aec.70058\">10.1111/aec.70058</a>.","apa":"Arpigiani, D., Aschero, V., Soler Schaller, R. M., &#38; Amoroso, M. M. (2025). A life-cycle approach to understand consequences of silvopastoral use on two native tree species of Northern Patagonia. <i>Austral Ecology</i>. Wiley. <a href=\"https://doi.org/10.1111/aec.70058\">https://doi.org/10.1111/aec.70058</a>","ama":"Arpigiani D, Aschero V, Soler Schaller RM, Amoroso MM. A life-cycle approach to understand consequences of silvopastoral use on two native tree species of Northern Patagonia. <i>Austral Ecology</i>. 2025;50(4). doi:<a href=\"https://doi.org/10.1111/aec.70058\">10.1111/aec.70058</a>","chicago":"Arpigiani, Daniela, Valeria Aschero, Rosina Matilde Soler Schaller, and Mariano M. Amoroso. “A Life-Cycle Approach to Understand Consequences of Silvopastoral Use on Two Native Tree Species of Northern Patagonia.” <i>Austral Ecology</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/aec.70058\">https://doi.org/10.1111/aec.70058</a>."},"date_updated":"2025-09-30T12:31:04Z","scopus_import":"1","_id":"19671","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"4","day":"01","article_number":"e70058","acknowledgement":"We would like to express our sincere gratitude to the owners of the estates, Lisandro and Oscar Lanfré, Roberto Criado and Yayo Tillería, for allowing us to conduct our research on their properties and for generously sharing their time and knowledge throughout these years. We are also deeply thankful to our field assistants, Matías Scotti, Clara Pissolito, Noel Szudruk, Mariano Varela, Ian Mott, Brisa Guenuleo, Nicolás Bistolfi, Facundo Gómez and Belén Vallerga, who tirelessly collaborated in the arduous tasks of monitoring and data collection, even in challenging weather conditions. We are grateful to CONICET for providing the doctoral scholarship to D. Arpigiani. This study received partial financial support from the Agencia MINCyT (PICT 2015-1692) and the Universidad Nacional de Río Negro (PI 40-B-478), Argentina.","publication_status":"published","author":[{"full_name":"Arpigiani, Daniela","last_name":"Arpigiani","first_name":"Daniela"},{"last_name":"Aschero","first_name":"Valeria","full_name":"Aschero, Valeria"},{"full_name":"Soler Schaller, Rosina Matilde","id":"9e668447-8c32-11ed-b0c7-8dc2d7b80803","first_name":"Rosina Matilde","last_name":"Soler Schaller"},{"full_name":"Amoroso, Mariano M.","first_name":"Mariano M.","last_name":"Amoroso"}],"department":[{"_id":"NiBa"}],"date_created":"2025-05-11T22:02:41Z","external_id":{"isi":["001476761500001"]},"status":"public","month":"04","type":"journal_article"},{"ddc":["570"],"oa_version":"Published Version","article_type":"original","year":"2025","doi":"10.1093/evolut/qpaf047","quality_controlled":"1","acknowledged_ssus":[{"_id":"ScienComp"}],"has_accepted_license":"1","publication_identifier":{"issn":["0014-3820"],"eissn":["1558-5646"]},"article_processing_charge":"Yes (via OA deal)","language":[{"iso":"eng"}],"abstract":[{"text":"Assortative mating and sexual selection are widespread in nature and can play an important role in speciation by facilitating the buildup and maintenance of reproductive isolation (RI). However, their contribution to genome-wide suppression of gene flow during RI is rarely quantified.\r\nHere, we consider a polygenic “magic” trait that is divergently selected across two populations connected by migration, while also serving as the basis of assortative mating, thus generating sexual selection on one or both sexes. We obtain theoretical predictions for divergence at\r\nindividual trait loci by assuming that the effect of all other loci on any locus can be encapsulated via an effective migration rate, which bears a simple relationship to measurable fitness components of migrants and various early-generation hybrids. Our analysis clarifies how “tipping\r\npoints” (characterized by an abrupt collapse of adaptive divergence) arise, and when assortative mating can shift the critical level of migration beyond which divergence collapses. We quantify the relative contributions of viability and sexual selection to genome-wide barriers to gene\r\nflow and discuss how these depend on existing divergence levels. Our results suggest that effective migration rates provide a useful way of understanding genomic divergence, even in scenarios involving multiple, interacting mechanisms of RI. ","lang":"eng"}],"publisher":"Oxford University Press","volume":79,"date_published":"2025-07-01T00:00:00Z","publication":"Evolution","intvolume":"        79","OA_type":"hybrid","publication_status":"published","related_material":{"record":[{"relation":"research_data","id":"18712","status":"public"}]},"author":[{"full_name":"Surendranadh, Parvathy","last_name":"Surendranadh","orcid":"0000-0001-6395-386X","first_name":"Parvathy","id":"455235B8-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Sachdeva, Himani","first_name":"Himani","last_name":"Sachdeva"}],"department":[{"_id":"NiBa"}],"external_id":{"isi":["001490646300001"]},"date_created":"2025-06-23T13:51:00Z","status":"public","tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"month":"07","type":"journal_article","page":"1185-1198","file":[{"access_level":"open_access","relation":"main_file","file_name":"2025_Evolution_Surendranadh.pdf","date_created":"2025-12-30T08:43:33Z","date_updated":"2025-12-30T08:43:33Z","file_id":"20898","checksum":"288ca936cef794d68a55356e70671846","success":1,"file_size":2784295,"content_type":"application/pdf","creator":"dernst"}],"isi":1,"corr_author":"1","OA_place":"publisher","title":"Effect of assortative mating and sexual selection on polygenic barriers to gene flow","citation":{"ieee":"P. Surendranadh and H. Sachdeva, “Effect of assortative mating and sexual selection on polygenic barriers to gene flow,” <i>Evolution</i>, vol. 79, no. 7. Oxford University Press, pp. 1185–1198, 2025.","mla":"Surendranadh, Parvathy, and Himani Sachdeva. “Effect of Assortative Mating and Sexual Selection on Polygenic Barriers to Gene Flow.” <i>Evolution</i>, vol. 79, no. 7, Oxford University Press, 2025, pp. 1185–98, doi:<a href=\"https://doi.org/10.1093/evolut/qpaf047\">10.1093/evolut/qpaf047</a>.","short":"P. Surendranadh, H. Sachdeva, Evolution 79 (2025) 1185–1198.","ista":"Surendranadh P, Sachdeva H. 2025. Effect of assortative mating and sexual selection on polygenic barriers to gene flow. Evolution. 79(7), 1185–1198.","ama":"Surendranadh P, Sachdeva H. Effect of assortative mating and sexual selection on polygenic barriers to gene flow. <i>Evolution</i>. 2025;79(7):1185-1198. doi:<a href=\"https://doi.org/10.1093/evolut/qpaf047\">10.1093/evolut/qpaf047</a>","chicago":"Surendranadh, Parvathy, and Himani Sachdeva. “Effect of Assortative Mating and Sexual Selection on Polygenic Barriers to Gene Flow.” <i>Evolution</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/evolut/qpaf047\">https://doi.org/10.1093/evolut/qpaf047</a>.","apa":"Surendranadh, P., &#38; Sachdeva, H. (2025). Effect of assortative mating and sexual selection on polygenic barriers to gene flow. <i>Evolution</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/evolut/qpaf047\">https://doi.org/10.1093/evolut/qpaf047</a>"},"oa":1,"date_updated":"2025-12-30T08:44:13Z","file_date_updated":"2025-12-30T08:43:33Z","scopus_import":"1","_id":"19876","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"7","day":"01","acknowledgement":"We thank Nick Barton for useful comments on the manuscript. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by Scientific Computing (SciComp)."},{"volume":34,"publication":"Molecular Ecology","date_published":"2025-11-01T00:00:00Z","intvolume":"        34","OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)","language":[{"iso":"eng"}],"abstract":[{"text":"Speciation is rarely observable directly. A way forward is to compare pairs of ecotypes that evolved in parallel in similar contexts but have reached different degrees of reproductive isolation. Such comparisons are possible in the marine snail Littorina saxatilis by contrasting barriers to gene flow between parallel ecotypes in Spain and Sweden. In both countries, divergent ecotypes have evolved to withstand either crab predation or wave action. Here, we explore transects spanning contact zones between the Crab and the Wave ecotypes using low-coverage whole-genome sequencing, morphological and behavioural traits. Despite parallel phenotypic divergence, distinct patterns of differentiation between the ecotypes emerged: a continuous cline in Sweden indicating a weak barrier to gene flow, but two highly genetically and phenotypically divergent, and partly spatially overlapping clusters in Spain suggesting a much stronger barrier to gene flow. The absence of Spanish early-generation hybrids supported strong isolation, but a low level of gene flow is evident from molecular data. In both countries, highly differentiated loci were located in both shared and country-specific chromosomal inversions but were also present in collinear regions. Despite being considered the same species and showing similar levels of phenotypic divergence, the Spanish ecotypes are much closer to full reproductive isolation than the Swedish ones. Barriers to gene flow of very different strengths between ecotypes within the same species might be explained by dissimilarities in the spatial arrangement of habitats, the selection gradients or the ages of the systems.","lang":"eng"}],"publisher":"Wiley","doi":"10.1111/mec.70025","quality_controlled":"1","has_accepted_license":"1","publication_identifier":{"issn":["0962-1083"],"eissn":["1365-294X"]},"oa_version":"Published Version","ddc":["570"],"article_type":"original","year":"2025","scopus_import":"1","file_date_updated":"2025-12-30T09:25:17Z","_id":"20102","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"21","article_number":"e70025","day":"01","acknowledgement":"This study was supported by European Research Council grant 693030-BARRIERS to RKB; the Swedish Research Council (grant number 2021-04191) to KJ; the Portuguese Foundation for Science and Technology (FCT: 2020.00275.CEECIND and PTDC/BIA-EVL/1614/2021) to RF; grant PID2022-137935NB-I00 by MICIU/AEI/ 10.13039/501100011033/and ERDF/EU (ED431C 2020-05) to JG, grant PID2021-124930NB-I00 funded by MICIU/AEI/ 10.13039/501100011033/and ERDF/EU to ERA, Xunta de Galicia (ED431C 2024/22), Centro singular de Investigación de Galicia accreditation 2024-2027 (ED431G 2023/07), ‘ERDF A way of making Europe’ and Norwegian Research Council RCN, project 315287 to AMW.","isi":1,"OA_place":"publisher","title":"Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail","oa":1,"citation":{"ista":"Raffini F, De Jode A, Johannesson K, Faria R, Zagrodzka ZB, Westram AM, Galindo J, Rolán-Alvarez E, Butlin RK. 2025. Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail. Molecular Ecology. 34(21), e70025.","short":"F. Raffini, A. De Jode, K. Johannesson, R. Faria, Z.B. Zagrodzka, A.M. Westram, J. Galindo, E. Rolán-Alvarez, R.K. Butlin, Molecular Ecology 34 (2025).","ieee":"F. Raffini <i>et al.</i>, “Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail,” <i>Molecular Ecology</i>, vol. 34, no. 21. Wiley, 2025.","mla":"Raffini, Francesca, et al. “Phenotypic Divergence and Genomic Architecture between Parallel Ecotypes at Two Different Points on the Speciation Continuum in a Marine Snail.” <i>Molecular Ecology</i>, vol. 34, no. 21, e70025, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/mec.70025\">10.1111/mec.70025</a>.","apa":"Raffini, F., De Jode, A., Johannesson, K., Faria, R., Zagrodzka, Z. B., Westram, A. M., … Butlin, R. K. (2025). Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail. <i>Molecular Ecology</i>. Wiley. <a href=\"https://doi.org/10.1111/mec.70025\">https://doi.org/10.1111/mec.70025</a>","ama":"Raffini F, De Jode A, Johannesson K, et al. Phenotypic divergence and genomic architecture between parallel ecotypes at two different points on the speciation continuum in a marine snail. <i>Molecular Ecology</i>. 2025;34(21). doi:<a href=\"https://doi.org/10.1111/mec.70025\">10.1111/mec.70025</a>","chicago":"Raffini, Francesca, Aurélien De Jode, Kerstin Johannesson, Rui Faria, Zuzanna B. Zagrodzka, Anja M Westram, Juan Galindo, Emilio Rolán-Alvarez, and Roger K. Butlin. “Phenotypic Divergence and Genomic Architecture between Parallel Ecotypes at Two Different Points on the Speciation Continuum in a Marine Snail.” <i>Molecular Ecology</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/mec.70025\">https://doi.org/10.1111/mec.70025</a>."},"date_updated":"2025-12-30T09:25:45Z","PlanS_conform":"1","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"status":"public","month":"11","type":"journal_article","file":[{"creator":"dernst","checksum":"ec01edda64cfbc6cbc8adf300f719644","file_id":"20906","success":1,"content_type":"application/pdf","file_size":2767745,"relation":"main_file","access_level":"open_access","date_updated":"2025-12-30T09:25:17Z","date_created":"2025-12-30T09:25:17Z","file_name":"2025_MolecEcology_Raffini.pdf"}],"publication_status":"published","author":[{"full_name":"Raffini, Francesca","first_name":"Francesca","last_name":"Raffini"},{"full_name":"De Jode, Aurélien","first_name":"Aurélien","last_name":"De Jode"},{"last_name":"Johannesson","first_name":"Kerstin","full_name":"Johannesson, Kerstin"},{"first_name":"Rui","last_name":"Faria","full_name":"Faria, Rui"},{"full_name":"Zagrodzka, Zuzanna B.","first_name":"Zuzanna B.","last_name":"Zagrodzka"},{"id":"3C147470-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1050-4969","first_name":"Anja M","last_name":"Westram","full_name":"Westram, Anja M"},{"full_name":"Galindo, Juan","last_name":"Galindo","first_name":"Juan"},{"last_name":"Rolán-Alvarez","first_name":"Emilio","full_name":"Rolán-Alvarez, Emilio"},{"full_name":"Butlin, Roger K.","first_name":"Roger K.","last_name":"Butlin"}],"department":[{"_id":"NiBa"}],"external_id":{"isi":["001538172800001"]},"date_created":"2025-08-03T22:01:31Z"},{"date_created":"2025-09-10T05:42:23Z","external_id":{"isi":["001542913000001"],"pmid":["40751392"]},"department":[{"_id":"NiBa"}],"pmid":1,"author":[{"first_name":"Thomas","orcid":"0000-0002-8511-0254","last_name":"Ellis","id":"3153D6D4-F248-11E8-B48F-1D18A9856A87","full_name":"Ellis, Thomas"},{"full_name":"Field, David","id":"419049E2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4014-8478","first_name":"David","last_name":"Field"},{"full_name":"Barton, Nicholas H","last_name":"Barton","first_name":"Nicholas H","orcid":"0000-0002-8548-5240","id":"4880FE40-F248-11E8-B48F-1D18A9856A87"}],"publication_status":"published","type":"journal_article","month":"09","file":[{"file_size":1698605,"content_type":"application/pdf","success":1,"file_id":"20911","checksum":"5059ad4d74e6327b84b5282a39d36774","creator":"dernst","file_name":"2025_MolecularEcology_Ellis.pdf","date_updated":"2025-12-30T10:12:17Z","date_created":"2025-12-30T10:12:17Z","access_level":"open_access","relation":"main_file"}],"tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"status":"public","date_updated":"2025-12-30T10:12:34Z","citation":{"ieee":"T. Ellis, D. Field, and N. H. Barton, “Joint estimation of paternity, sibships and pollen dispersal in a snapdragon hybrid zone,” <i>Molecular Ecology</i>, vol. 34, no. 15. Wiley, 2025.","mla":"Ellis, Thomas, et al. “Joint Estimation of Paternity, Sibships and Pollen Dispersal in a Snapdragon Hybrid Zone.” <i>Molecular Ecology</i>, vol. 34, no. 15, e70051, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/mec.70051\">10.1111/mec.70051</a>.","ista":"Ellis T, Field D, Barton NH. 2025. Joint estimation of paternity, sibships and pollen dispersal in a snapdragon hybrid zone. Molecular Ecology. 34(15), e70051.","short":"T. Ellis, D. Field, N.H. Barton, Molecular Ecology 34 (2025).","ama":"Ellis T, Field D, Barton NH. Joint estimation of paternity, sibships and pollen dispersal in a snapdragon hybrid zone. <i>Molecular Ecology</i>. 2025;34(15). doi:<a href=\"https://doi.org/10.1111/mec.70051\">10.1111/mec.70051</a>","chicago":"Ellis, Thomas, David Field, and Nicholas H Barton. “Joint Estimation of Paternity, Sibships and Pollen Dispersal in a Snapdragon Hybrid Zone.” <i>Molecular Ecology</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/mec.70051\">https://doi.org/10.1111/mec.70051</a>.","apa":"Ellis, T., Field, D., &#38; Barton, N. H. (2025). Joint estimation of paternity, sibships and pollen dispersal in a snapdragon hybrid zone. <i>Molecular Ecology</i>. Wiley. <a href=\"https://doi.org/10.1111/mec.70051\">https://doi.org/10.1111/mec.70051</a>"},"oa":1,"OA_place":"publisher","title":"Joint estimation of paternity, sibships and pollen dispersal in a snapdragon hybrid zone","isi":1,"corr_author":"1","article_number":"e70051","day":"02","acknowledgement":"We thank a large number of field volunteers for maintaining the population sampling, and Tom White for assistance with seed collection. We thank Sylvia Rebel for plating tissue for DNA extraction, as well as Sean Stankowski and two anonymous reviewers for feedback on the manuscript. ","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"15","_id":"20325","file_date_updated":"2025-12-30T10:12:17Z","scopus_import":"1","year":"2025","article_type":"original","oa_version":"Published Version","ddc":["570"],"publication_identifier":{"issn":["0962-1083"],"eissn":["1365-294X"]},"has_accepted_license":"1","doi":"10.1111/mec.70051","quality_controlled":"1","publisher":"Wiley","abstract":[{"text":"Inferring genealogical relationships of wild populations is useful because it gives direct estimates of mating patterns and variance in reproductive success. Inference can be improved by including information about parentage shared between siblings, or by modelling phenotypes or population data related to mating. However, we currently lack a framework to infer parent–offspring relationships, sibships and population parameters in a single analysis. To address this, we here extend a previous method, Fractional Analysis of Paternity and Sibships, to include population data for the case where one parent is known. We illustrate this with the example of pollen dispersal in a natural hybrid zone population of the snapdragon Antirrhinum majus. Pollen dispersal is leptokurtic, with half of mating events occurring within 30 m, but with a long tail of mating events up to 859 m. Using simulations, we find that both sibship and population information substantially improve pedigree reconstruction, and that we can expect to resolve median dispersal distances with high accuracy.","lang":"eng"}],"article_processing_charge":"Yes (via OA deal)","language":[{"iso":"eng"}],"intvolume":"        34","OA_type":"hybrid","volume":34,"publication":"Molecular Ecology","date_published":"2025-09-02T00:00:00Z"},{"department":[{"_id":"BeVi"},{"_id":"NiBa"}],"external_id":{"isi":["001598595000001"]},"date_created":"2025-09-10T05:48:04Z","publication_status":"published","author":[{"full_name":"Puixeu Sala, Gemma","orcid":"0000-0001-8330-1754","first_name":"Gemma","last_name":"Puixeu Sala","id":"33AB266C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Hayward, Laura","id":"fc885ee5-24bf-11eb-ad7b-bcc5104c0c1b","last_name":"Hayward","first_name":"Laura"}],"month":"11","type":"journal_article","file":[{"content_type":"application/pdf","file_size":1550562,"success":1,"checksum":"bbb73bbf8617812d4d8db4af92be9538","file_id":"20946","creator":"dernst","date_updated":"2026-01-05T13:03:18Z","date_created":"2026-01-05T13:03:18Z","file_name":"2025_Genetics_Puixeu.pdf","relation":"main_file","access_level":"open_access"}],"status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"project":[{"_id":"9B9DFC9E-BA93-11EA-9121-9846C619BF3A","name":"Sexual conflict: resolution, constraints and biomedical implications","grant_number":"25817"},{"grant_number":"665385","name":"International IST Doctoral Program","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"_id":"bd6958e0-d553-11ed-ba76-86eba6a76c00","name":"Understanding the evolution of continuous genomes","grant_number":"101055327"}],"citation":{"ista":"Puixeu Sala G, Hayward L. 2025. The relationship between sexual dimorphism and intersex correlation: Do models support intuition? Genetics. 231(3), iyaf175.","short":"G. Puixeu Sala, L. Hayward, Genetics 231 (2025).","mla":"Puixeu Sala, Gemma, and Laura Hayward. “The Relationship between Sexual Dimorphism and Intersex Correlation: Do Models Support Intuition?” <i>Genetics</i>, vol. 231, no. 3, iyaf175, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/genetics/iyaf175\">10.1093/genetics/iyaf175</a>.","ieee":"G. Puixeu Sala and L. Hayward, “The relationship between sexual dimorphism and intersex correlation: Do models support intuition?,” <i>Genetics</i>, vol. 231, no. 3. Oxford University Press, 2025.","apa":"Puixeu Sala, G., &#38; Hayward, L. (2025). The relationship between sexual dimorphism and intersex correlation: Do models support intuition? <i>Genetics</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/genetics/iyaf175\">https://doi.org/10.1093/genetics/iyaf175</a>","ama":"Puixeu Sala G, Hayward L. The relationship between sexual dimorphism and intersex correlation: Do models support intuition? <i>Genetics</i>. 2025;231(3). doi:<a href=\"https://doi.org/10.1093/genetics/iyaf175\">10.1093/genetics/iyaf175</a>","chicago":"Puixeu Sala, Gemma, and Laura Hayward. “The Relationship between Sexual Dimorphism and Intersex Correlation: Do Models Support Intuition?” <i>Genetics</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/genetics/iyaf175\">https://doi.org/10.1093/genetics/iyaf175</a>."},"oa":1,"date_updated":"2026-01-05T13:04:07Z","PlanS_conform":"1","corr_author":"1","isi":1,"OA_place":"publisher","title":"The relationship between sexual dimorphism and intersex correlation: Do models support intuition?","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"3","article_number":"iyaf175","day":"01","acknowledgement":"We thank Tim Connallon for useful discussions and correspondence, Himani Sachdeva and Nick Barton for comments on the manuscript and the Scientific Computing unit at ISTA for technical support. GP is the recipient of a DOC Fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology Austria (DOC 25817) and received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant (agreement no. 665385). LH received funding from the European Research Council, under the HaplotypeStructure Grant (grant no. 101055327) to Nick Barton.","file_date_updated":"2026-01-05T13:03:18Z","scopus_import":"1","_id":"20330","year":"2025","ddc":["570"],"oa_version":"Published Version","article_type":"original","publication_identifier":{"issn":["1943-2631"]},"doi":"10.1093/genetics/iyaf175","quality_controlled":"1","has_accepted_license":"1","abstract":[{"text":"The evolution of sexual dimorphism (the difference in average trait values between females and males, SD), is often thought to be constrained by shared genetic architecture between the sexes. Indeed, it is commonly expected that SD should negatively correlate with the intersex correlation (the genetic correlation between effects of segregating variants in females and males, r fm), either because (1) traits with ancestrally low r fm are less constrained in their ability to respond to sex-specific selection and thus evolve to be more dimorphic, or because (2) sex-specific selection, driving sexual dimorphism evolution, also acts to reduce r fm. Despite the intuitive appeal and prominence of these ideas, their generality and the conditions in which they hold remain unclear. Here, we develop models incorporating sex-specific stabilizing selection, mutation and genetic drift to examine the relationship between r fm and SD. We show that the two commonly-discussed mechanisms with the potential to generate a negative correlation between SD and r fm could just as easily generate a positive association, since the standard line of reasoning hinges on a hidden assumption that sex-specific adaptation more frequently favors increased dimorphism than reduced dimorphism. Our results provide, to our knowledge, the first mechanistic framework for understanding the conditions under which a correlation between r fm and SD may arise and offer a compelling explanation for inconsistent empirical evidence. We also make the intriguing observation that—even when selection between the two sexes is identical—drift generates nonzero SD. We quantify this effect and discuss its significance.","lang":"eng"}],"publisher":"Oxford University Press","article_processing_charge":"Yes (via OA deal)","language":[{"iso":"eng"}],"volume":231,"ec_funded":1,"publication":"Genetics","date_published":"2025-11-01T00:00:00Z","intvolume":"       231","OA_type":"hybrid"},{"citation":{"apa":"Bustamante, G. N., Arena, M. E., Selzer, L., Ruggirello, M., Rodríguez, P., Pedrazzani, S., … Soler Schaller, R. M. (2025). Biotic interactions between trees and colonizing shrubs: Implications for active restoration in southern Patagonian forests. <i>Plant Ecology</i>. Springer Nature. <a href=\"https://doi.org/10.1007/s11258-025-01568-0\">https://doi.org/10.1007/s11258-025-01568-0</a>","ama":"Bustamante GN, Arena ME, Selzer L, et al. Biotic interactions between trees and colonizing shrubs: Implications for active restoration in southern Patagonian forests. <i>Plant Ecology</i>. 2025;226:1301-1313. doi:<a href=\"https://doi.org/10.1007/s11258-025-01568-0\">10.1007/s11258-025-01568-0</a>","chicago":"Bustamante, Gimena Noemí, Miriam Elisabet Arena, Luciano Selzer, Matthew Ruggirello, Paula Rodríguez, Samuele Pedrazzani, Jose Antonio Navarro-Cano, and Rosina Matilde Soler Schaller. “Biotic Interactions between Trees and Colonizing Shrubs: Implications for Active Restoration in Southern Patagonian Forests.” <i>Plant Ecology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1007/s11258-025-01568-0\">https://doi.org/10.1007/s11258-025-01568-0</a>.","short":"G.N. Bustamante, M.E. Arena, L. Selzer, M. Ruggirello, P. Rodríguez, S. Pedrazzani, J.A. Navarro-Cano, R.M. Soler Schaller, Plant Ecology 226 (2025) 1301–1313.","ista":"Bustamante GN, Arena ME, Selzer L, Ruggirello M, Rodríguez P, Pedrazzani S, Navarro-Cano JA, Soler Schaller RM. 2025. Biotic interactions between trees and colonizing shrubs: Implications for active restoration in southern Patagonian forests. Plant Ecology. 226, 1301–1313.","mla":"Bustamante, Gimena Noemí, et al. “Biotic Interactions between Trees and Colonizing Shrubs: Implications for Active Restoration in Southern Patagonian Forests.” <i>Plant Ecology</i>, vol. 226, Springer Nature, 2025, pp. 1301–13, doi:<a href=\"https://doi.org/10.1007/s11258-025-01568-0\">10.1007/s11258-025-01568-0</a>.","ieee":"G. N. Bustamante <i>et al.</i>, “Biotic interactions between trees and colonizing shrubs: Implications for active restoration in southern Patagonian forests,” <i>Plant Ecology</i>, vol. 226. Springer Nature, pp. 1301–1313, 2025."},"date_updated":"2026-01-05T13:23:57Z","isi":1,"title":"Biotic interactions between trees and colonizing shrubs: Implications for active restoration in southern Patagonian forests","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"01","scopus_import":"1","_id":"20429","department":[{"_id":"NiBa"}],"external_id":{"isi":["001581599800001"]},"date_created":"2025-10-05T22:01:36Z","publication_status":"published","author":[{"full_name":"Bustamante, Gimena Noemí","last_name":"Bustamante","first_name":"Gimena Noemí"},{"full_name":"Arena, Miriam Elisabet","last_name":"Arena","first_name":"Miriam Elisabet"},{"last_name":"Selzer","first_name":"Luciano","full_name":"Selzer, Luciano"},{"last_name":"Ruggirello","first_name":"Matthew","full_name":"Ruggirello, Matthew"},{"full_name":"Rodríguez, Paula","last_name":"Rodríguez","first_name":"Paula"},{"full_name":"Pedrazzani, Samuele","last_name":"Pedrazzani","first_name":"Samuele"},{"full_name":"Navarro-Cano, Jose Antonio","first_name":"Jose Antonio","last_name":"Navarro-Cano"},{"full_name":"Soler Schaller, Rosina Matilde","first_name":"Rosina Matilde","last_name":"Soler Schaller","id":"9e668447-8c32-11ed-b0c7-8dc2d7b80803"}],"month":"12","type":"journal_article","page":"1301-1313","status":"public","abstract":[{"text":"Plant–plant interactions are key to understanding ecosystem services and shaping restoration strategies, as they can produce either negative or positive effects, determining species establishment and growth. Recognizing these interactions during early-life stages provides valuable insights for restoration in human-disturbed areas. One promising approach is nucleation planting, which establishes small clusters of native species in strategically selected sites, being particularly useful in sites with large herbivores. In southern Patagonia, livestock production has historically been the main economic activity, severely impacting extensive areas of Nothofagus antarctica forest through grazing and intentional burning to increase forage. In this context, nucleation planting with Berberis microphylla, a non-palatable shrub, could foster forest recovery in degraded sites. To evaluate this, we conducted an experiment testing the response of trees to varying shrub number, while also assessing intraspecific effects in both species. We measured survival, biomass, and functional traits. Results showed that the combination of four shrubs surrounding a single tree maintained tree survival at levels comparable to trees growing alone, while seedlings exhibited conspecific negative plant number dependence. Additionally, B. microphylla increased its below- to above-ground biomass ratio under higher plant number, indicating resource reallocation and niche differentiation through spatial separation of root systems.","lang":"eng"}],"publisher":"Springer Nature","article_processing_charge":"No","language":[{"iso":"eng"}],"volume":226,"date_published":"2025-12-01T00:00:00Z","publication":"Plant Ecology","intvolume":"       226","OA_type":"closed access","year":"2025","oa_version":"None","article_type":"original","publication_identifier":{"issn":["1385-0237"],"eissn":["1573-5052"]},"doi":"10.1007/s11258-025-01568-0","quality_controlled":"1"},{"publication_identifier":{"eissn":["1558-5646"]},"quality_controlled":"1","doi":"10.1093/evolut/qpaf143","year":"2025","article_type":"original","oa_version":"Preprint","OA_type":"green","intvolume":"        79","publication":"Evolution","date_published":"2025-10-17T00:00:00Z","volume":79,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.01.22.634382"}],"publisher":"Oxford University Press","abstract":[{"lang":"eng","text":"Genetic drift is potentially an important component of selection for sex, as it is a source of statistical associations between alleles at selected loci. By increasing local drift, population structure may thus amplify the evolutionary advantage of sex. However, most previous models have focused either on haploid populations or on diploid populations without spatial structure. In this article, we use two- and three-locus analytical models and multilocus simulations to explore selection for sex in a diploid population structured according to the island model, in the presence of recurrent deleterious mutations. Our results show that selection generally favors an intermediate rate of sex that decreases as the direct cost of sex increases and increases moderately as the degree of population structure increases. Selection for sex is generated by multiple effects involving genetic associations within and between loci. When selection occurs at many loci, it is generally dominated by interference effects involving deleterious alleles at different loci, captured by our three-locus model. In our multilocus simulations, we observed an irreversible spread of asexual mutants under strong costs of sex, and when deleterious mutations are partially recessive. However, population structure may prevent this spread of asexual mutants when dispersal rates are sufficiently small."}],"language":[{"iso":"eng"}],"article_processing_charge":"No","page":"2167-2180","type":"journal_article","month":"10","status":"public","external_id":{"pmid":["40668071"],"isi":["001547542300001"]},"date_created":"2025-10-26T23:01:34Z","department":[{"_id":"NiBa"}],"author":[{"id":"1676e173-8143-11ed-8927-fe165216a93f","first_name":"Louise","orcid":"0000-0003-0371-9339","last_name":"Fouqueau","full_name":"Fouqueau, Louise"},{"last_name":"Roze","first_name":"Denis","full_name":"Roze, Denis"}],"pmid":1,"publication_status":"published","acknowledgement":"L.F. is funded by the NOMIS-ISTA Fellowship Program. We thank Colin Olito and two anonymous reviewers for helpful comments, and the bioinformatics and computing services at Roscoff’s Biological Station (Abims platform) and at Institute of Science and Technology Austria for computing time.","day":"17","issue":"10","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"20531","scopus_import":"1","date_updated":"2025-12-01T15:03:54Z","oa":1,"citation":{"ama":"Fouqueau L, Roze D. Deleterious mutations and selection for sex in spatially structured, diploid populations. <i>Evolution</i>. 2025;79(10):2167-2180. doi:<a href=\"https://doi.org/10.1093/evolut/qpaf143\">10.1093/evolut/qpaf143</a>","chicago":"Fouqueau, Louise, and Denis Roze. “Deleterious Mutations and Selection for Sex in Spatially Structured, Diploid Populations.” <i>Evolution</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/evolut/qpaf143\">https://doi.org/10.1093/evolut/qpaf143</a>.","apa":"Fouqueau, L., &#38; Roze, D. (2025). Deleterious mutations and selection for sex in spatially structured, diploid populations. <i>Evolution</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/evolut/qpaf143\">https://doi.org/10.1093/evolut/qpaf143</a>","mla":"Fouqueau, Louise, and Denis Roze. “Deleterious Mutations and Selection for Sex in Spatially Structured, Diploid Populations.” <i>Evolution</i>, vol. 79, no. 10, Oxford University Press, 2025, pp. 2167–80, doi:<a href=\"https://doi.org/10.1093/evolut/qpaf143\">10.1093/evolut/qpaf143</a>.","ieee":"L. Fouqueau and D. Roze, “Deleterious mutations and selection for sex in spatially structured, diploid populations,” <i>Evolution</i>, vol. 79, no. 10. Oxford University Press, pp. 2167–2180, 2025.","short":"L. Fouqueau, D. Roze, Evolution 79 (2025) 2167–2180.","ista":"Fouqueau L, Roze D. 2025. Deleterious mutations and selection for sex in spatially structured, diploid populations. Evolution. 79(10), 2167–2180."},"project":[{"_id":"9B861AAC-BA93-11EA-9121-9846C619BF3A","name":"NOMIS Fellowship Program"}],"title":"Deleterious mutations and selection for sex in spatially structured, diploid populations","OA_place":"repository","isi":1},{"oa":1,"citation":{"ista":"Pal A. 2025. Using genealogies to study the genomic basis of species divergence. Institute of Science and Technology Austria.","short":"A. Pal, Using Genealogies to Study the Genomic Basis of Species Divergence, Institute of Science and Technology Austria, 2025.","ieee":"A. Pal, “Using genealogies to study the genomic basis of species divergence,” Institute of Science and Technology Austria, 2025.","mla":"Pal, Arka. <i>Using Genealogies to Study the Genomic Basis of Species Divergence</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20694\">10.15479/AT-ISTA-20694</a>.","apa":"Pal, A. (2025). <i>Using genealogies to study the genomic basis of species divergence</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20694\">https://doi.org/10.15479/AT-ISTA-20694</a>","chicago":"Pal, Arka. “Using Genealogies to Study the Genomic Basis of Species Divergence.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20694\">https://doi.org/10.15479/AT-ISTA-20694</a>.","ama":"Pal A. Using genealogies to study the genomic basis of species divergence. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20694\">10.15479/AT-ISTA-20694</a>"},"project":[{"name":"Understanding the evolution of continuous genomes","grant_number":"101055327","_id":"bd6958e0-d553-11ed-ba76-86eba6a76c00"},{"_id":"05959E1C-7A3F-11EA-A408-12923DDC885E","grant_number":"P32166","name":"Snapdragon Speciation"}],"date_updated":"2026-04-28T13:20:36Z","corr_author":"1","title":"Using genealogies to study the genomic basis of species divergence","OA_place":"publisher","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","day":"25","supervisor":[{"id":"4880FE40-F248-11E8-B48F-1D18A9856A87","last_name":"Barton","orcid":"0000-0002-8548-5240","first_name":"Nicholas H","full_name":"Barton, Nicholas H"}],"file_date_updated":"2026-03-01T23:30:03Z","_id":"20694","department":[{"_id":"GradSch"},{"_id":"NiBa"}],"date_created":"2025-11-25T13:19:11Z","related_material":{"record":[{"relation":"part_of_dissertation","id":"12159","status":"public"},{"status":"public","id":"14796","relation":"part_of_dissertation"},{"status":"public","id":"20190","relation":"part_of_dissertation"}]},"publication_status":"published","author":[{"first_name":"Arka","orcid":"0000-0002-4530-8469","last_name":"Pal","id":"6AAB2240-CA9A-11E9-9C1A-D9D1E5697425","full_name":"Pal, Arka"}],"alternative_title":["ISTA Thesis"],"file":[{"creator":"apal","checksum":"7a10a738d58524aebb5dcbd9b34c21c5","file_id":"20721","file_size":42723135,"content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_name":"2025_Pal_Arka_Thesis.pdf","embargo":"2026-03-01","date_updated":"2026-03-01T23:30:03Z","date_created":"2025-12-01T13:53:36Z"},{"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_size":60632116,"checksum":"166d832b08d0434ce407f8f3cb930fe5","file_id":"20722","embargo_to":"open_access","creator":"apal","date_updated":"2026-03-01T23:30:03Z","date_created":"2025-12-01T13:53:39Z","file_name":"2025_Pal_Arka_Thesis.docx","relation":"source_file","access_level":"closed"}],"page":"268","type":"dissertation","month":"11","tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"status":"public","degree_awarded":"PhD","abstract":[{"lang":"eng","text":"Understanding the mechanisms underlying speciation is a central aim of evolutionary biology.\r\nA persistent challenge in the field is to identify loci that contribute to reproductive isolation,\r\nwhile disentangling signals of selection from demography, linkage and intrinsic genomic\r\nfeatures. Traditional population genomic approaches that rely on site-based statistics in\r\narbitrary fixed windows face inherent limitations, as they conflate historical and\r\ncontemporary processes of divergence and overlook haplotype structure. Recent advances in\r\nwhole-genome sequencing and methods to infer ancestral recombination graphs (ARGs) now\r\noffer the opportunity to study genealogical relationships explicitly, revealing how lineages\r\ncoalesce and recombine through time. By directly analysing haplotype clustering by species\r\nor phenotype and their patterns of coalescence, ARG-based methods show promise for\r\ndiagnosing sweeps, identifying barrier loci maintained under divergent selection amid gene\r\nflow, and tracing their evolutionary history.\r\nIn this thesis, I explore the utility of genealogical approaches for studying species\r\ndivergence. In chapter 2, I propose a conceptual framework for defining haplotype blocks\r\nthrough the structure of the ARG, using simulations and empirical data to highlight how\r\ngenealogical processes generate rich and often overlooked haplotypic patterns.\r\nIn chapter 3, I examine the genomic basis of a key evolutionary innovation in marine\r\nsnails Littorina. These snails offer a unique opportunity to study an innovation because they\r\ninclude a very recent transition from egg-laying to live bearing, yet snails with the different\r\nreproductive modes are not reciprocally monophyletic. I exploited this by using topology\r\nclustering in ARG-derived local genealogical trees to pinpoint narrow genomic regions or\r\nhaplotype blocks that carry swept alleles, thus revealing that the transition from egg-laying\r\nto live-bearing involves multiple, live-bearer-specific sweeps.\r\nChapter 4 establishes a population-scale, phased genomic resource for Antirrhinum\r\nmajus, using cost-effective haplotagging, then optimizes imputation from low-coverage data\r\nagainst high-accuracy KASP sequencing to maximize sequence completeness with modest\r\naccuracy trade-offs against a traditional short-read sequence pipeline. A hybrid phasing\r\nstrategy combines molecular phasing with statistical phasing to generate phased whole\r\ngenome sequences of 1084 Antirrhinum individuals at a fraction of long-read sequencing\r\ncosts.\r\nIn chapter 5, I analyse hybridising populations from two replicate hybrid zones to find\r\na parallel genetic basis of flower colour, amidst the noise in genomic differentiation landscape\r\ndriven by variation in demographic history. While outlier genome scans of FST failed to dissect\r\nthe causes of differentiation, ARG-based topology clustering revealed a reuse of colour\r\nassociated haplotypes across hybrid zones. In addition to the biological insight, this chapter\r\nalso presents a comparison of the latest ARG inference tools, showing that signals of\r\nAbstract\r\nviii\r\ntopological clustering qualitatively agree between methods, despite differences in the tree\r\nsequences.\r\nNext, in chapter 6, by leveraging ~1000 individuals in one of the hybrid zones, I\r\nintegrated genome-wide association studies of floral pigmentation with genealogical\r\ninference, to test for additional colour loci, and confirm the effect of previously described loci.\r\nThis work demonstrates that flower colour variation is driven by a small number of large effect\r\nloci, while also hinting at the presence of a new candidate regulatory factor.\r\nFinally in chapter 7, in a preliminary analysis, I begin to dissect the genomic island of\r\nspeciation around Rosea/Eluta to understand its evolutionary origins. My results show that it\r\nconsists of 5 highly divergent loci, each of which is associated with flower colour. Using\r\npatterns of coalescence in genealogical trees, I find evidence of staggered selective sweeps\r\nand a persistent localized barrier to gene flow within an otherwise permeable genome.\r\nTogether, these chapters add to the increasing pool of studies using genealogical\r\napproaches to complement and extend site-based statistics to use haplotype structures in\r\nspeciation research. By tracking haplotypes directly and connecting genealogical clustering to\r\npopulation processes, ARG-based inference promises to provide new insights into how local\r\nselective pressures, demographic history, and long-term barriers interact to shape the\r\ngenomic architecture of divergence. By underscoring the value of ARGs in revealing the finescale origins and maintenance of biodiversity, this thesis presents cautious optimism about\r\nthe benefits of using genealogical inference to learn more than what site-based statistics\r\ncould tell us."}],"publisher":"Institute of Science and Technology Austria","language":[{"iso":"eng"}],"article_processing_charge":"No","date_published":"2025-11-25T00:00:00Z","year":"2025","oa_version":"Published Version","ddc":["576","578"],"publication_identifier":{"issn":["2663-337X"]},"acknowledged_ssus":[{"_id":"ScienComp"}],"doi":"10.15479/AT-ISTA-20694","has_accepted_license":"1"},{"acknowledged_ssus":[{"_id":"ScienComp"}],"quality_controlled":"1","doi":"10.1111/mec.70067","has_accepted_license":"1","publication_identifier":{"eissn":["1365-294X"],"issn":["0962-1083"]},"ddc":["570"],"oa_version":"Published Version","article_type":"original","year":"2025","publication":"Molecular Ecology","date_published":"2025-11-01T00:00:00Z","volume":34,"OA_type":"hybrid","intvolume":"        34","language":[{"iso":"eng"}],"article_processing_charge":"Yes (via OA deal)","abstract":[{"lang":"eng","text":"A major goal of speciation research is identifying loci that underpin barriers to gene flow. Population genomics takes a ‘bottom-up’ approach, scanning the genome for molecular signatures of processes that drive or maintain divergence. However, interpreting the ‘genomic landscape’ of speciation is complicated, because genome scans conflate multiple processes, most of which are not informative about gene flow. However, studying replicated population contrasts, including multiple incidences of secondary contact, can strengthen inferences. In this paper, we use linked-read sequencing (haplotagging), FST scans and genealogical methods to characterise the genomic landscape associated with replicate hybrid zone formation. We studied two flower colour varieties of the common snapdragon, Antirrhinum majus subspecies majus, that form secondary hybrid zones in multiple independent valleys in the Pyrenees. Consistent with past work, we found very low differentiation at one well-studied zone (Planoles). However, at a second zone (Avellanet), we found stronger differentiation and greater heterogeneity, which we argue is due to differences in the amount of introgression following secondary contact. Topology weighting of genealogical trees identified loci where haplotype diversity was associated with the two snapdragon varieties. Two of the strongest associations were at previously identified flower colour loci: Flavia, that affects yellow pigmentation, and Rosea/Eluta, two linked loci that affect magenta pigmentation. Preliminary analysis of coalescence times provides additional evidence for selective sweeps at these loci and barriers to gene flow. Our study highlights the impact of demographic history on the differentiation landscape, emphasising the need to distinguish between historical divergence and recent introgression."}],"publisher":"Wiley","status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"access_level":"open_access","relation":"main_file","file_name":"2025_MolecEcology_Pal.pdf","date_updated":"2026-01-05T13:47:47Z","date_created":"2026-01-05T13:47:47Z","creator":"dernst","file_id":"20958","checksum":"c586fc674df4e7dd6e43aef87a52c6f6","success":1,"file_size":9886694,"content_type":"application/pdf"}],"month":"11","type":"journal_article","related_material":{"record":[{"id":"20694","status":"public","relation":"dissertation_contains"}],"link":[{"url":"https://ista.ac.at/en/news/snapdragon-secrets/","relation":"press_release","description":"News on ISTA website"}]},"publication_status":"published","author":[{"full_name":"Pal, Arka","last_name":"Pal","first_name":"Arka","orcid":"0000-0002-4530-8469","id":"6AAB2240-CA9A-11E9-9C1A-D9D1E5697425"},{"full_name":"Shipilina, Daria","id":"428A94B0-F248-11E8-B48F-1D18A9856A87","last_name":"Shipilina","first_name":"Daria","orcid":"0000-0002-1145-9226"},{"full_name":"Le Moan, Alan","last_name":"Le Moan","first_name":"Alan"},{"first_name":"Adrian J.","last_name":"Mcnairn","full_name":"Mcnairn, Adrian J."},{"first_name":"Jennifer K.","last_name":"Grenier","full_name":"Grenier, Jennifer K."},{"full_name":"Kucka, Marek","last_name":"Kucka","first_name":"Marek"},{"last_name":"Coop","first_name":"Graham","full_name":"Coop, Graham"},{"full_name":"Chan, Yingguang Frank","first_name":"Yingguang Frank","last_name":"Chan"},{"full_name":"Barton, Nicholas H","orcid":"0000-0002-8548-5240","first_name":"Nicholas H","last_name":"Barton","id":"4880FE40-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Field, David","orcid":"0000-0002-4014-8478","first_name":"David","last_name":"Field","id":"419049E2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Stankowski, Sean","id":"43161670-5719-11EA-8025-FABC3DDC885E","first_name":"Sean","last_name":"Stankowski"}],"department":[{"_id":"NiBa"}],"date_created":"2025-08-17T22:01:37Z","external_id":{"isi":["001546622100001"]},"scopus_import":"1","file_date_updated":"2026-01-05T13:47:47Z","_id":"20190","issue":"22","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","acknowledgement":"We thank ESEB Godfrey Hewitt Mobility Award for supporting AP’s research stay at UC Davis. We thank Tom Ellis, Parvathy Surendranadh, and other Barton Group and Coop Lab members for stimulating discussions. We are grateful to all the interns and volunteers who have helped us with fieldwork. We thank Eva Salmerón Mateu for her assistance in fieldwork logistics at the field station, El Serrat. We are grateful to Enrico Coen and his research group for providing the Antirrhinum molle PoolSeq data used in the allele polarisation. We are also thankful to Enrico Coen and Cristophe Thébaud for discovering the Avellanet hybrid zone, followed up with sampling led by D.L.F. in 2017. The study was supported by Austrian Science Fund (FWF) Grant (Snapdragon Speciation P32166, awarded to D.L.F.); ERC (Advanced Grant HaplotypeStructure 101055327, awarded to NHB); ERC (POC Grant 101069216, awarded to Y.F.C.) and the National Institutes of Health (NIH R35 GM136290, awarded to G.C.). Y.F.C. was supported by the Max Planck Society. Computing infrastructure for bioinformatics and analyses was provided by ISTA High Performance Cluster. ","article_number":"e70067","day":"01","isi":1,"corr_author":"1","title":"Genealogical analysis of replicate flower colour hybrid zones in Antirrhinum","OA_place":"publisher","oa":1,"citation":{"apa":"Pal, A., Shipilina, D., Le Moan, A., Mcnairn, A. J., Grenier, J. K., Kucka, M., … Stankowski, S. (2025). Genealogical analysis of replicate flower colour hybrid zones in Antirrhinum. <i>Molecular Ecology</i>. Wiley. <a href=\"https://doi.org/10.1111/mec.70067\">https://doi.org/10.1111/mec.70067</a>","chicago":"Pal, Arka, Daria Shipilina, Alan Le Moan, Adrian J. Mcnairn, Jennifer K. Grenier, Marek Kucka, Graham Coop, et al. “Genealogical Analysis of Replicate Flower Colour Hybrid Zones in Antirrhinum.” <i>Molecular Ecology</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/mec.70067\">https://doi.org/10.1111/mec.70067</a>.","ama":"Pal A, Shipilina D, Le Moan A, et al. Genealogical analysis of replicate flower colour hybrid zones in Antirrhinum. <i>Molecular Ecology</i>. 2025;34(22). doi:<a href=\"https://doi.org/10.1111/mec.70067\">10.1111/mec.70067</a>","short":"A. Pal, D. Shipilina, A. Le Moan, A.J. Mcnairn, J.K. Grenier, M. Kucka, G. Coop, Y.F. Chan, N.H. Barton, D. Field, S. Stankowski, Molecular Ecology 34 (2025).","ista":"Pal A, Shipilina D, Le Moan A, Mcnairn AJ, Grenier JK, Kucka M, Coop G, Chan YF, Barton NH, Field D, Stankowski S. 2025. Genealogical analysis of replicate flower colour hybrid zones in Antirrhinum. Molecular Ecology. 34(22), e70067.","mla":"Pal, Arka, et al. “Genealogical Analysis of Replicate Flower Colour Hybrid Zones in Antirrhinum.” <i>Molecular Ecology</i>, vol. 34, no. 22, e70067, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/mec.70067\">10.1111/mec.70067</a>.","ieee":"A. Pal <i>et al.</i>, “Genealogical analysis of replicate flower colour hybrid zones in Antirrhinum,” <i>Molecular Ecology</i>, vol. 34, no. 22. Wiley, 2025."},"project":[{"grant_number":"P32166","name":"Snapdragon Speciation","_id":"05959E1C-7A3F-11EA-A408-12923DDC885E"},{"name":"Understanding the evolution of continuous genomes","grant_number":"101055327","_id":"bd6958e0-d553-11ed-ba76-86eba6a76c00"}],"PlanS_conform":"1","date_updated":"2026-08-02T22:30:45Z"},{"main_file_link":[{"url":"https://doi.org/10.1101/2024.10.15.618444","open_access":"1"}],"date_updated":"2026-06-12T12:43:34Z","project":[{"name":"The impact of deleterious mutations on small populations","grant_number":"26293","_id":"34d33d68-11ca-11ed-8bc3-ec13763c0ca8"},{"_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","name":"IST-BRIDGE: International postdoctoral program","call_identifier":"H2020","grant_number":"101034413"}],"citation":{"ista":"Khudiakova K, Boenkost F, Tourniaire JN. Genealogies under purifying selection. bioRxiv, <a href=\"https://doi.org/10.1101/2024.10.15.618444\">10.1101/2024.10.15.618444</a>.","short":"K. Khudiakova, F. Boenkost, J.N. Tourniaire, BioRxiv (n.d.).","ieee":"K. Khudiakova, F. Boenkost, and J. N. Tourniaire, “Genealogies under purifying selection,” <i>bioRxiv</i>. .","mla":"Khudiakova, Kseniia, et al. “Genealogies under Purifying Selection.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2024.10.15.618444\">10.1101/2024.10.15.618444</a>.","apa":"Khudiakova, K., Boenkost, F., &#38; Tourniaire, J. N. (n.d.). Genealogies under purifying selection. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2024.10.15.618444\">https://doi.org/10.1101/2024.10.15.618444</a>","chicago":"Khudiakova, Kseniia, Florin Boenkost, and Julie N Tourniaire. “Genealogies under Purifying Selection.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2024.10.15.618444\">https://doi.org/10.1101/2024.10.15.618444</a>.","ama":"Khudiakova K, Boenkost F, Tourniaire JN. Genealogies under purifying selection. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2024.10.15.618444\">10.1101/2024.10.15.618444</a>"},"oa":1,"abstract":[{"text":"Selection against deleterious mutations, called purifying selection, plays a central role in evolution and acts in all populations. It is known that the genetic patterns observed in genomic regions undergoing purifying selection differ from those resulting from neutral evolution. However, a comprehensive understanding of the underlying mechanisms shaping those patterns is still lacking.\r\n\r\nIn the present work, we use simulations combined with a genealogical approach to identify the effect of purifying selection on the ancestry and thus on the genetic diversity. Our analysis relies on the postulate that the genealogy belongs to the universality class of Beta-coalescents. Under this assumption, we derive statistics measuring the distortion of the genealogy. This approach allows us to consider a wide range of regimes (i.e. arbitrary selection and mutation strengths) and uncover a rich phase diagram. We find that, for strong selection, the limiting genealogy is given by Kingman’s coalescent on a polynomial timescale. As selection gets weaker, Muller’s ratchet starts operating, setting off the emergence of multiple mergers in the genealogical structures. Our results show that while multiple-merger coalescents are often interpreted as the signature of selective sweeps in rapidly adapting populations, these structures can also appear in the context of Muller’s ratchet.","lang":"eng"}],"article_processing_charge":"No","language":[{"iso":"eng"}],"title":"Genealogies under purifying selection","corr_author":"1","day":"18","acknowledgement":"This work was supported by the Austrian Academy of Science, DOC fellowship No 26293 (K.K.) and the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 101034413 (J.T.). Simulations were performed on the ISTA High-performance Computing Cluster.","OA_type":"green","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","ec_funded":1,"publication":"bioRxiv","date_published":"2024-10-18T00:00:00Z","_id":"21967","date_created":"2026-06-09T12:14:08Z","year":"2024","department":[{"_id":"NiBa"},{"_id":"JaMa"}],"author":[{"full_name":"Khudiakova, Kseniia","id":"4E6DC800-AE37-11E9-AC72-31CAE5697425","orcid":"0000-0002-6246-1465","first_name":"Kseniia","last_name":"Khudiakova"},{"full_name":"Boenkost, Florin","last_name":"Boenkost","first_name":"Florin"},{"full_name":"Tourniaire, Julie N","last_name":"Tourniaire","first_name":"Julie N","id":"5dc06dd8-8e51-11ec-9170-8d9c450cc216"}],"publication_status":"draft","related_material":{"record":[{"relation":"dissertation_contains","id":"21918","status":"public"}]},"oa_version":"Preprint","month":"10","type":"preprint","tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"status":"public","doi":"10.1101/2024.10.15.618444"}]