---
_id: '7473'
abstract:
- lang: eng
text: How structural and functional properties of synapses relate to each other
is a fundamental question in neuroscience. Electrophysiology has elucidated mechanisms
of synaptic transmission, and electron microscopy (EM) has provided insight into
morphological properties of synapses. Here we describe an enhanced method for
functional EM (“flash and freeze”), combining optogenetic stimulation with high-pressure
freezing. We demonstrate that the improved method can be applied to intact networks
in acute brain slices and organotypic slice cultures from mice. As a proof of
concept, we probed vesicle pool changes during synaptic transmission at the hippocampal
mossy fiber-CA3 pyramidal neuron synapse. Our findings show overlap of the docked
vesicle pool and the functionally defined readily releasable pool and provide
evidence of fast endocytosis at this synapse. Functional EM with acute slices
and slice cultures has the potential to reveal the structural and functional mechanisms
of transmission in intact, genetically perturbed, and disease-affected synapses.
acknowledgement: This project has received funding from the European Research Council
(ERC) and European Commission (EC), under the European Union’s Horizon 2020 research
and innovation programme (ERC grant agreement No. 692692 and Marie Sklodowska-Curie
708497) and from Fonds zur Förderung der Wissenschaftlichen Forschung (Z 312-B27
Wittgenstein award and DK W1205-B09). We thank Johann Danzl and Ryuichi Shigemoto
for critically reading the manuscript; Walter Kaufmann, Daniel Gutl, and Vanessa
Zheden for extensive EM training, advice, and experimental assistance; Benjamin
Suter for substantial help with light stimulation, ImageJ plugins for analysis,
and manuscript editing; Florian Marr and Christina Altmutter for technical support;
Eleftheria Kralli-Beller for manuscript editing; Julia König and Paul Wurzinger
(Leica Microsystems) for helpful technical discussions; and Taija Makinen for providing
the Prox1-CreERT2 mouse line.
article_processing_charge: No
article_type: original
author:
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Olena
full_name: Kim, Olena
id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
last_name: Kim
orcid: 0000-0003-2344-1039
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Borges Merjane C, Kim O, Jonas PM. Functional electron microscopy (“Flash and
Freeze”) of identified cortical synapses in acute brain slices. Neuron.
2020;105:992-1006. doi:10.1016/j.neuron.2019.12.022
apa: Borges Merjane, C., Kim, O., & Jonas, P. M. (2020). Functional electron
microscopy (“Flash and Freeze”) of identified cortical synapses in acute brain
slices. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2019.12.022
chicago: Borges Merjane, Carolina, Olena Kim, and Peter M Jonas. “Functional Electron
Microscopy (‘Flash and Freeze’) of Identified Cortical Synapses in Acute Brain
Slices.” Neuron. Elsevier, 2020. https://doi.org/10.1016/j.neuron.2019.12.022.
ieee: C. Borges Merjane, O. Kim, and P. M. Jonas, “Functional electron microscopy
(‘Flash and Freeze’) of identified cortical synapses in acute brain slices,” Neuron,
vol. 105. Elsevier, pp. 992–1006, 2020.
ista: Borges Merjane C, Kim O, Jonas PM. 2020. Functional electron microscopy (“Flash
and Freeze”) of identified cortical synapses in acute brain slices. Neuron. 105,
992–1006.
mla: Borges Merjane, Carolina, et al. “Functional Electron Microscopy (‘Flash and
Freeze’) of Identified Cortical Synapses in Acute Brain Slices.” Neuron,
vol. 105, Elsevier, 2020, pp. 992–1006, doi:10.1016/j.neuron.2019.12.022.
short: C. Borges Merjane, O. Kim, P.M. Jonas, Neuron 105 (2020) 992–1006.
corr_author: '1'
date_created: 2020-02-10T15:59:45Z
date_published: 2020-03-18T00:00:00Z
date_updated: 2026-10-05T22:30:05Z
day: '18'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2019.12.022
ec_funded: 1
external_id:
isi:
- '000520854700008'
pmid:
- '31928842'
file:
- access_level: open_access
checksum: 3582664addf26859e86ac5bec3e01416
content_type: application/pdf
creator: dernst
date_created: 2020-11-20T08:58:53Z
date_updated: 2020-11-20T08:58:53Z
file_id: '8778'
file_name: 2020_Neuron_BorgesMerjane.pdf
file_size: 9712957
relation: main_file
success: 1
file_date_updated: 2020-11-20T08:58:53Z
fulldoi: https://doi.org/10.1016/j.neuron.2019.12.022
has_accepted_license: '1'
intvolume: ' 105'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '03'
oa: 1
oa_version: Published Version
page: 992-1006
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: 25BAF7B2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '708497'
name: Presynaptic calcium channels distribution and impact on coupling at the hippocampal
mossy fiber synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: Synaptic communication in neuronal microcircuits
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W01205
name: Zellkommunikation in Gesundheit und Krankheit
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/flash-and-freeze-reveals-dynamics-of-nerve-connections/
record:
- id: '11196'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Functional electron microscopy (“Flash and Freeze”) of identified cortical
synapses in acute brain slices
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 105
year: '2020'
...
---
OA_type: closed access
_id: '19989'
abstract:
- lang: ger
text: Neurone empfangen Eingangssignale, konvertieren diese in Aktionspotenziale
und generieren schließlich Ausgangssignale auf ihren Zielzellen. Dabei sind die
zu überwindenden räumlichen Distanzen oft groß. Daher ist entscheidend, dass elektrische
Signale in Nervenzellen schnell von einem zum anderen Ort geleitet werden können.
Diese wichtige Aufgabe erfüllt das Axon, der „Ausgangsfortsatz“ der Nervenzelle.
Für die schnelle Leitung des Aktionspotenzials sind sowohl die passiven Eigenschaften
des axonalen Kabels als auch die aktiven Eigenschaften der Zellmembran von entscheidender
Bedeutung. Die Evolution bedient sich zweier Tricks, um die Leitungsgeschwindigkeit
des Aktionspotenzials zu maximieren. Der eine Trick ist die Zunahme des Axondurchmessers.
Der andere Trick ist die Ausbildung von Markscheiden. Dies führt bei nahezu gleichem
Platzbedarf zu einer Zunahme der Leistungsgeschwindigkeit um fast zwei Größenordnungen.
Die Aktionspotenzialleitung an myelinisierten Axonen erfolgt „saltatorisch“.
article_processing_charge: No
author:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Jonas PM. Aktionspotenzial: Fortleitung im Axon. In: Physiologie des Menschen.
32nd ed. Springer-Lehrbuch. Berlin, Heidelberg: Springer Nature; 2019:72-82. doi:10.1007/978-3-662-56468-4_7'
apa: 'Jonas, P. M. (2019). Aktionspotenzial: Fortleitung im Axon. In Physiologie
des Menschen (32nd ed., pp. 72–82). Berlin, Heidelberg: Springer Nature. https://doi.org/10.1007/978-3-662-56468-4_7'
chicago: 'Jonas, Peter M. “Aktionspotenzial: Fortleitung im Axon.” In Physiologie
des Menschen, 32nd ed., 72–82. Springer-Lehrbuch. Berlin, Heidelberg: Springer
Nature, 2019. https://doi.org/10.1007/978-3-662-56468-4_7.'
ieee: 'P. M. Jonas, “Aktionspotenzial: Fortleitung im Axon,” in Physiologie des
Menschen, 32nd ed., Berlin, Heidelberg: Springer Nature, 2019, pp. 72–82.'
ista: 'Jonas PM. 2019.Aktionspotenzial: Fortleitung im Axon. In: Physiologie des
Menschen. , 72–82.'
mla: 'Jonas, Peter M. “Aktionspotenzial: Fortleitung im Axon.” Physiologie des
Menschen, 32nd ed., Springer Nature, 2019, pp. 72–82, doi:10.1007/978-3-662-56468-4_7.'
short: P.M. Jonas, in:, Physiologie des Menschen, 32nd ed., Springer Nature, Berlin,
Heidelberg, 2019, pp. 72–82.
corr_author: '1'
date_created: 2025-07-10T13:36:36Z
date_published: 2019-04-02T00:00:00Z
date_updated: 2025-09-23T11:44:57Z
day: '02'
department:
- _id: PeJo
doi: 10.1007/978-3-662-56468-4_7
edition: '32'
fulldoi: https://doi.org/10.1007/978-3-662-56468-4_7
language:
- iso: ger
month: '04'
oa_version: None
page: 72-82
place: Berlin, Heidelberg
publication: Physiologie des Menschen
publication_identifier:
eisbn:
- '9783662564684'
eissn:
- 2512-5214
isbn:
- '9783662564677'
issn:
- 0937-7433
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
series_title: Springer-Lehrbuch
status: public
title: 'Aktionspotenzial: Fortleitung im Axon'
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2019'
...
---
_id: '7405'
abstract:
- lang: eng
text: Biophysical modeling of neuronal networks helps to integrate and interpret
rapidly growing and disparate experimental datasets at multiple scales. The NetPyNE
tool (www.netpyne.org) provides both programmatic and graphical interfaces to
develop data-driven multiscale network models in NEURON. NetPyNE clearly separates
model parameters from implementation code. Users provide specifications at a high
level via a standardized declarative language, for example connectivity rules,
to create millions of cell-to-cell connections. NetPyNE then enables users to
generate the NEURON network, run efficiently parallelized simulations, optimize
and explore network parameters through automated batch runs, and use built-in
functions for visualization and analysis – connectivity matrices, voltage traces,
spike raster plots, local field potentials, and information theoretic measures.
NetPyNE also facilitates model sharing by exporting and importing standardized
formats (NeuroML and SONATA). NetPyNE is already being used to teach computational
neuroscience students and by modelers to investigate brain regions and phenomena.
article_number: e44494
article_processing_charge: No
article_type: original
author:
- first_name: Salvador
full_name: Dura-Bernal, Salvador
last_name: Dura-Bernal
- first_name: Benjamin
full_name: Suter, Benjamin
id: 4952F31E-F248-11E8-B48F-1D18A9856A87
last_name: Suter
orcid: 0000-0002-9885-6936
- first_name: Padraig
full_name: Gleeson, Padraig
last_name: Gleeson
- first_name: Matteo
full_name: Cantarelli, Matteo
last_name: Cantarelli
- first_name: Adrian
full_name: Quintana, Adrian
last_name: Quintana
- first_name: Facundo
full_name: Rodriguez, Facundo
last_name: Rodriguez
- first_name: David J
full_name: Kedziora, David J
last_name: Kedziora
- first_name: George L
full_name: Chadderdon, George L
last_name: Chadderdon
- first_name: Cliff C
full_name: Kerr, Cliff C
last_name: Kerr
- first_name: Samuel A
full_name: Neymotin, Samuel A
last_name: Neymotin
- first_name: Robert A
full_name: McDougal, Robert A
last_name: McDougal
- first_name: Michael
full_name: Hines, Michael
last_name: Hines
- first_name: Gordon MG
full_name: Shepherd, Gordon MG
last_name: Shepherd
- first_name: William W
full_name: Lytton, William W
last_name: Lytton
citation:
ama: Dura-Bernal S, Suter B, Gleeson P, et al. NetPyNE, a tool for data-driven multiscale
modeling of brain circuits. eLife. 2019;8. doi:10.7554/elife.44494
apa: Dura-Bernal, S., Suter, B., Gleeson, P., Cantarelli, M., Quintana, A., Rodriguez,
F., … Lytton, W. W. (2019). NetPyNE, a tool for data-driven multiscale modeling
of brain circuits. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.44494
chicago: Dura-Bernal, Salvador, Benjamin Suter, Padraig Gleeson, Matteo Cantarelli,
Adrian Quintana, Facundo Rodriguez, David J Kedziora, et al. “NetPyNE, a Tool
for Data-Driven Multiscale Modeling of Brain Circuits.” ELife. eLife Sciences
Publications, 2019. https://doi.org/10.7554/elife.44494.
ieee: S. Dura-Bernal et al., “NetPyNE, a tool for data-driven multiscale
modeling of brain circuits,” eLife, vol. 8. eLife Sciences Publications,
2019.
ista: Dura-Bernal S, Suter B, Gleeson P, Cantarelli M, Quintana A, Rodriguez F,
Kedziora DJ, Chadderdon GL, Kerr CC, Neymotin SA, McDougal RA, Hines M, Shepherd
GM, Lytton WW. 2019. NetPyNE, a tool for data-driven multiscale modeling of brain
circuits. eLife. 8, e44494.
mla: Dura-Bernal, Salvador, et al. “NetPyNE, a Tool for Data-Driven Multiscale Modeling
of Brain Circuits.” ELife, vol. 8, e44494, eLife Sciences Publications,
2019, doi:10.7554/elife.44494.
short: S. Dura-Bernal, B. Suter, P. Gleeson, M. Cantarelli, A. Quintana, F. Rodriguez,
D.J. Kedziora, G.L. Chadderdon, C.C. Kerr, S.A. Neymotin, R.A. McDougal, M. Hines,
G.M. Shepherd, W.W. Lytton, ELife 8 (2019).
date_created: 2020-01-30T09:08:01Z
date_published: 2019-05-31T00:00:00Z
date_updated: 2023-09-07T14:27:52Z
day: '31'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.7554/elife.44494
external_id:
isi:
- '000468968400001'
pmid:
- '31025934'
file:
- access_level: open_access
checksum: 7014189c11c10a12feeeae37f054871d
content_type: application/pdf
creator: dernst
date_created: 2020-02-04T08:41:47Z
date_updated: 2020-07-14T12:47:57Z
file_id: '7444'
file_name: 2019_eLife_DuraBernal.pdf
file_size: 6182359
relation: main_file
file_date_updated: 2020-07-14T12:47:57Z
fulldoi: https://doi.org/10.7554/elife.44494
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: NetPyNE, a tool for data-driven multiscale modeling of brain circuits
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2019'
...
---
_id: '11222'
acknowledgement: This work was supported by the ERC and EU Horizon 2020 (ERC 692692;
MSC-IF 708497) and FWF Z 312-B27 Wittgenstein award; W 1205-B09).
article_number: A3.27
article_processing_charge: No
author:
- first_name: Olena
full_name: Kim, Olena
id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
last_name: Kim
orcid: 0000-0003-2344-1039
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Kim O, Borges Merjane C, Jonas PM. Functional analysis of the docked vesicle
pool in hippocampal mossy fiber terminals by electron microscopy. In: Intrinsic
Activity. Vol 7. Austrian Pharmacological Society; 2019. doi:10.25006/ia.7.s1-a3.27'
apa: 'Kim, O., Borges Merjane, C., & Jonas, P. M. (2019). Functional analysis
of the docked vesicle pool in hippocampal mossy fiber terminals by electron microscopy.
In Intrinsic Activity (Vol. 7). Innsbruck, Austria: Austrian Pharmacological
Society. https://doi.org/10.25006/ia.7.s1-a3.27'
chicago: Kim, Olena, Carolina Borges Merjane, and Peter M Jonas. “Functional Analysis
of the Docked Vesicle Pool in Hippocampal Mossy Fiber Terminals by Electron Microscopy.”
In Intrinsic Activity, Vol. 7. Austrian Pharmacological Society, 2019.
https://doi.org/10.25006/ia.7.s1-a3.27.
ieee: O. Kim, C. Borges Merjane, and P. M. Jonas, “Functional analysis of the docked
vesicle pool in hippocampal mossy fiber terminals by electron microscopy,” in
Intrinsic Activity, Innsbruck, Austria, 2019, vol. 7, no. Suppl. 1.
ista: 'Kim O, Borges Merjane C, Jonas PM. 2019. Functional analysis of the docked
vesicle pool in hippocampal mossy fiber terminals by electron microscopy. Intrinsic
Activity. ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological
Society vol. 7, A3.27.'
mla: Kim, Olena, et al. “Functional Analysis of the Docked Vesicle Pool in Hippocampal
Mossy Fiber Terminals by Electron Microscopy.” Intrinsic Activity, vol.
7, no. Suppl. 1, A3.27, Austrian Pharmacological Society, 2019, doi:10.25006/ia.7.s1-a3.27.
short: O. Kim, C. Borges Merjane, P.M. Jonas, in:, Intrinsic Activity, Austrian
Pharmacological Society, 2019.
conference:
end_date: 2019-09-27
location: Innsbruck, Austria
name: 'ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological
Society'
start_date: 2019-09-25
corr_author: '1'
date_created: 2022-04-20T15:06:05Z
date_published: 2019-09-11T00:00:00Z
date_updated: 2026-10-05T22:30:05Z
day: '11'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.25006/ia.7.s1-a3.27
ec_funded: 1
fulldoi: https://doi.org/10.25006/ia.7.s1-a3.27
intvolume: ' 7'
issue: Suppl. 1
keyword:
- hippocampus
- mossy fibers
- readily releasable pool
- electron microscopy
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.intrinsicactivity.org/2019/7/S1/A3.27/
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: 25BAF7B2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '708497'
name: Presynaptic calcium channels distribution and impact on coupling at the hippocampal
mossy fiber synapse
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W01205
name: Zellkommunikation in Gesundheit und Krankheit
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: Synaptic communication in neuronal microcircuits
publication: Intrinsic Activity
publication_identifier:
issn:
- 2309-8503
publication_status: published
publisher: Austrian Pharmacological Society
quality_controlled: '1'
related_material:
record:
- id: '11196'
relation: dissertation_contains
status: public
status: public
title: Functional analysis of the docked vesicle pool in hippocampal mossy fiber terminals
by electron microscopy
type: conference_abstract
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2019'
...
---
OA_place: publisher
_id: '6363'
abstract:
- lang: eng
text: "Distinguishing between similar experiences is achieved by the brain
\ in a process called pattern separation. In the hippocampus, pattern
\ separation reduces the interference of memories and increases the storage
capacity by decorrelating similar inputs patterns of neuronal activity into
\ non-overlapping output firing patterns. Winners-take-all (WTA) mechanism
\ is a theoretical model for pattern separation in which a \"winner\"
\ cell suppresses the activity of the neighboring neurons through feedback
inhibition. However, if the network properties of the dentate gyrus support WTA
as a biologically conceivable model remains unknown. Here, we showed that the
connectivity rules of PV+interneurons and their synaptic properties are optimizedfor
efficient pattern separation. We found using multiple whole-cell in vitrorecordings
that PV+interneurons mainly connect to granule cells (GC) through lateral inhibition,
a form of feedback inhibition in which a GC inhibits other GCs but not
\ itself through the activation of PV+interneurons. Thus, lateral inhibition
between GC–PV+interneurons was ~10 times more abundant than recurrent connections.
Furthermore, the GC–PV+interneuron connectivity was more spatially confined
\ but less abundant than PV+interneurons–GC connectivity, leading to an
\ asymmetrical distribution of excitatory and inhibitory connectivity. Our
network model of the dentate gyrus with incorporated real connectivity rules efficiently
decorrelates neuronal activity patterns using WTA as the primary mechanism.
\ This process relied on lateral inhibition, fast-signaling properties of
\ PV+interneurons and the asymmetrical distribution of excitatory and inhibitory
connectivity. Finally, we found that silencing the activity of PV+interneurons
in vivoleads to acute deficits in discrimination between similar environments,
suggesting that PV+interneuron networks are necessary for behavioral relevant
computations. Our results demonstrate that PV+interneurons possess unique
connectivity and fast signaling properties that confer to the dentate
\ gyrus network properties that allow the emergence of pattern separation. Thus,
our results contribute to the knowledge of how specific forms of network organization
underlie sophisticated types of information processing. \r\n"
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: 'Claudia '
full_name: 'Espinoza Martinez, Claudia '
id: 31FFEE2E-F248-11E8-B48F-1D18A9856A87
last_name: Espinoza Martinez
orcid: 0000-0003-4710-2082
citation:
ama: Espinoza Martinez C. Parvalbumin+ interneurons enable efficient pattern separation
in hippocampal microcircuits. 2019. doi:10.15479/AT:ISTA:6363
apa: Espinoza Martinez, C. (2019). Parvalbumin+ interneurons enable efficient
pattern separation in hippocampal microcircuits. Institute of Science and
Technology Austria. https://doi.org/10.15479/AT:ISTA:6363
chicago: Espinoza Martinez, Claudia . “Parvalbumin+ Interneurons Enable Efficient
Pattern Separation in Hippocampal Microcircuits.” Institute of Science and Technology
Austria, 2019. https://doi.org/10.15479/AT:ISTA:6363.
ieee: C. Espinoza Martinez, “Parvalbumin+ interneurons enable efficient pattern
separation in hippocampal microcircuits,” Institute of Science and Technology
Austria, 2019.
ista: Espinoza Martinez C. 2019. Parvalbumin+ interneurons enable efficient pattern
separation in hippocampal microcircuits. Institute of Science and Technology Austria.
mla: Espinoza Martinez, Claudia. Parvalbumin+ Interneurons Enable Efficient Pattern
Separation in Hippocampal Microcircuits. Institute of Science and Technology
Austria, 2019, doi:10.15479/AT:ISTA:6363.
short: C. Espinoza Martinez, Parvalbumin+ Interneurons Enable Efficient Pattern
Separation in Hippocampal Microcircuits, Institute of Science and Technology Austria,
2019.
corr_author: '1'
date_created: 2019-04-30T11:56:10Z
date_published: 2019-04-30T00:00:00Z
date_updated: 2026-04-08T13:57:19Z
day: '30'
ddc:
- '570'
degree_awarded: PhD
department:
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status: public
supervisor:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
title: Parvalbumin+ interneurons enable efficient pattern separation in hippocampal
microcircuits
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2019'
...
---
_id: '320'
abstract:
- lang: eng
text: 'Fast-spiking, parvalbumin-expressing GABAergic interneurons (PV+-BCs) express
a complex machinery of rapid signaling mechanisms, including specialized voltage-gated
ion channels to generate brief action potentials (APs). However, short APs are
associated with overlapping Na+ and K+ fluxes and are therefore energetically
expensive. How the potentially vicious combination of high AP frequency and inefficient
spike generation can be reconciled with limited energy supply is presently unclear.
To address this question, we performed direct recordings from the PV+-BC axon,
the subcellular structure where active conductances for AP initiation and propagation
are located. Surprisingly, the energy required for the AP was, on average, only
∼1.6 times the theoretical minimum. High energy efficiency emerged from the combination
of fast inactivation of Na+ channels and delayed activation of Kv3-type K+ channels,
which minimized ion flux overlap during APs. Thus, the complementary tuning of
axonal Na+ and K+ channel gating optimizes both fast signaling properties and
metabolic efficiency. Hu et al. demonstrate that action potentials in parvalbumin-expressing
GABAergic interneuron axons are energetically efficient, which is highly unexpected
given their brief duration. High energy efficiency emerges from the combination
of fast inactivation of voltage-gated Na+ channels and delayed activation of Kv3
channels in the axon. '
article_processing_charge: Yes (in subscription journal)
author:
- first_name: Hua
full_name: Hu, Hua
id: 4AC0145C-F248-11E8-B48F-1D18A9856A87
last_name: Hu
- first_name: Fabian
full_name: Roth, Fabian
last_name: Roth
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Hu H, Roth F, Vandael DH, Jonas PM. Complementary tuning of Na+ and K+ channel
gating underlies fast and energy-efficient action potentials in GABAergic interneuron
axons. Neuron. 2018;98(1):156-165. doi:10.1016/j.neuron.2018.02.024
apa: Hu, H., Roth, F., Vandael, D. H., & Jonas, P. M. (2018). Complementary
tuning of Na+ and K+ channel gating underlies fast and energy-efficient action
potentials in GABAergic interneuron axons. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2018.02.024
chicago: Hu, Hua, Fabian Roth, David H Vandael, and Peter M Jonas. “Complementary
Tuning of Na+ and K+ Channel Gating Underlies Fast and Energy-Efficient Action
Potentials in GABAergic Interneuron Axons.” Neuron. Elsevier, 2018. https://doi.org/10.1016/j.neuron.2018.02.024.
ieee: H. Hu, F. Roth, D. H. Vandael, and P. M. Jonas, “Complementary tuning of Na+
and K+ channel gating underlies fast and energy-efficient action potentials in
GABAergic interneuron axons,” Neuron, vol. 98, no. 1. Elsevier, pp. 156–165,
2018.
ista: Hu H, Roth F, Vandael DH, Jonas PM. 2018. Complementary tuning of Na+ and
K+ channel gating underlies fast and energy-efficient action potentials in GABAergic
interneuron axons. Neuron. 98(1), 156–165.
mla: Hu, Hua, et al. “Complementary Tuning of Na+ and K+ Channel Gating Underlies
Fast and Energy-Efficient Action Potentials in GABAergic Interneuron Axons.” Neuron,
vol. 98, no. 1, Elsevier, 2018, pp. 156–65, doi:10.1016/j.neuron.2018.02.024.
short: H. Hu, F. Roth, D.H. Vandael, P.M. Jonas, Neuron 98 (2018) 156–165.
corr_author: '1'
date_created: 2018-12-11T11:45:48Z
date_published: 2018-04-04T00:00:00Z
date_updated: 2025-04-15T08:29:04Z
day: '04'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2018.02.024
ec_funded: 1
external_id:
isi:
- '000429192100016'
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month: '04'
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oa_version: Published Version
page: 156 - 165
project:
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call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: Synaptic communication in neuronal microcircuits
publication: Neuron
publication_status: published
publisher: Elsevier
publist_id: '7545'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/a-certain-type-of-neurons-is-more-energy-efficient-than-previously-assumed/
scopus_import: '1'
status: public
title: Complementary tuning of Na+ and K+ channel gating underlies fast and energy-efficient
action potentials in GABAergic interneuron axons
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 98
year: '2018'
...
---
OA_place: publisher
_id: '324'
abstract:
- lang: eng
text: Neuronal networks in the brain consist of two main types of neuron, glutamatergic
principal neurons and GABAergic interneurons. Although these interneurons only
represent 10–20% of the whole population, they mediate feedback and feedforward
inhibition and are involved in the generation of high-frequency network oscillations.
A hallmark functional property of GABAergic interneurons, especially of the parvalbumin‑expressing
(PV+) subtypes, is the speed of signaling at their output synapse across species
and brain regions. Several molecular and subcellular factors may underlie the
submillisecond signaling at GABAergic synapses. Such as the selective use of P/Q
type Ca2+ channels and the tight coupling between Ca2+ channels and Ca2+ sensors
of exocytosis. However, whether the molecular identity of the release sensor contributes
to these signaling properties remains unclear. Besides, these interneurons are
mainly show depression in response to train of stimuli. How could they keep sufficient
release to control the activity of postsynaptic principal neurons during high
network activity, is largely elusive. For my Ph.D. work, we firstly examined the
Ca2+ sensor of exocytosis at the GABAergic basket cell (BC) to Purkinje cell (PC)
synapse in the cerebellum. Immunolabeling suggested that BC terminals selectively
expressed synaptotagmin 2 (Syt2), whereas synaptotagmin 1 (Syt1) was enriched
in excitatory terminals. Genetic elimination of Syt2 reduced action potential-evoked
release to ~10% compared to the wild-type control, identifying Syt2 as the major
Ca2+ sensor at BC‑PC synapses. Differential adenovirus-mediated rescue revealed
Syt2 triggered release with shorter latency and higher temporal precision, and
mediated faster vesicle pool replenishment than Syt1. Furthermore, deletion of
Syt2 severely reduced and delayed disynaptic inhibition following parallel fiber
stimulation. Thus, the selective use of Syt2 as the release sensor at BC–PC synapse
ensures fast feedforward inhibition in cerebellar microcircuits. Additionally,
we tested the function of another synaptotagmin member, Syt7, for inhibitory synaptic
transmission at the BC–PC synapse. Syt7 is thought to be a Ca2+ sensor that mediates
asynchronous transmitter release and facilitation at synapses. However, it is
strongly expressed in fast-spiking, PV+ GABAergic interneurons and the output
synapses of these neurons produce only minimal asynchronous release and show depression
rather than facilitation. How could Syt7, a facilitation sensor, contribute to
the depressed inhibitory synaptic transmission needs to be further investigated
and understood. Our results indicated that at the BC–PC synapse, Syt7 contributes
to asynchronous release, pool replenishment and facilitation. In combination,
these three effects ensure efficient transmitter release during high‑frequency
activity and guarantee frequency independence of inhibition. Taken together, our
results confirmed that Syt2, which has the fastest kinetic properties among all
synaptotagmin members, is mainly used by the inhibitory BC‑PC synapse for synaptic
transmission, contributing to the speed and temporal precision of transmitter
release. Furthermore, we showed that Syt7, another highly expressed synaptotagmin
member in the output synapses of cerebellar BCs, is used for ensuring efficient
inhibitor synaptic transmission during high activity.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
citation:
ama: Chen C. Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter
release. 2018. doi:10.15479/AT:ISTA:th_997
apa: Chen, C. (2018). Synaptotagmins ensure speed and efficiency of inhibitory
neurotransmitter release. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_997
chicago: Chen, Chong. “Synaptotagmins Ensure Speed and Efficiency of Inhibitory
Neurotransmitter Release.” Institute of Science and Technology Austria, 2018.
https://doi.org/10.15479/AT:ISTA:th_997.
ieee: C. Chen, “Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter
release,” Institute of Science and Technology Austria, 2018.
ista: Chen C. 2018. Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter
release. Institute of Science and Technology Austria.
mla: Chen, Chong. Synaptotagmins Ensure Speed and Efficiency of Inhibitory Neurotransmitter
Release. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_997.
short: C. Chen, Synaptotagmins Ensure Speed and Efficiency of Inhibitory Neurotransmitter
Release, Institute of Science and Technology Austria, 2018.
corr_author: '1'
date_created: 2018-12-11T11:45:49Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2026-04-08T14:09:29Z
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degree_awarded: PhD
department:
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oa: 1
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issn:
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publist_id: '7541'
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related_material:
record:
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relation: part_of_dissertation
status: public
- id: '749'
relation: part_of_dissertation
status: public
status: public
supervisor:
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full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
title: Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter release
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2018'
...
---
_id: '21'
abstract:
- lang: eng
text: Parvalbumin-positive (PV+) GABAergic interneurons in hippocampal microcircuits
are thought to play a key role in several higher network functions, such as feedforward
and feedback inhibition, network oscillations, and pattern separation. Fast lateral
inhibition mediated by GABAergic interneurons may implement a winner-takes-all
mechanism in the hippocampal input layer. However, it is not clear whether the
functional connectivity rules of granule cells (GCs) and interneurons in the dentate
gyrus are consistent with such a mechanism. Using simultaneous patch-clamp recordings
from up to seven GCs and up to four PV+ interneurons in the dentate gyrus, we
find that connectivity is structured in space, synapse-specific, and enriched
in specific disynaptic motifs. In contrast to the neocortex, lateral inhibition
in the dentate gyrus (in which a GC inhibits neighboring GCs via a PV+ interneuron)
is ~ 10-times more abundant than recurrent inhibition (in which a GC inhibits
itself). Thus, unique connectivity rules may enable the dentate gyrus to perform
specific higher-order computations
acknowledgement: This project received funding from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation programme
(grant agreement No 692692) and the Fond zur Förderung der Wissenschaftlichen Forschung
(Z 312-B27, Wittgenstein award), both to P.J..
article_number: '4605'
article_processing_charge: No
article_type: original
author:
- first_name: 'Claudia '
full_name: 'Espinoza Martinez, Claudia '
id: 31FFEE2E-F248-11E8-B48F-1D18A9856A87
last_name: Espinoza Martinez
orcid: 0000-0003-4710-2082
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
orcid: 0000-0003-2209-5242
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-0256-6529
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Espinoza Martinez C, Guzmán J, Zhang X, Jonas PM. Parvalbumin+ interneurons
obey unique connectivity rules and establish a powerful lateral-inhibition microcircuit
in dentate gyrus. Nature Communications. 2018;9(1). doi:10.1038/s41467-018-06899-3
apa: Espinoza Martinez, C., Guzmán, J., Zhang, X., & Jonas, P. M. (2018). Parvalbumin+
interneurons obey unique connectivity rules and establish a powerful lateral-inhibition
microcircuit in dentate gyrus. Nature Communications. Nature Publishing
Group. https://doi.org/10.1038/s41467-018-06899-3
chicago: Espinoza Martinez, Claudia , José Guzmán, Xiaomin Zhang, and Peter M Jonas.
“Parvalbumin+ Interneurons Obey Unique Connectivity Rules and Establish a Powerful
Lateral-Inhibition Microcircuit in Dentate Gyrus.” Nature Communications.
Nature Publishing Group, 2018. https://doi.org/10.1038/s41467-018-06899-3.
ieee: C. Espinoza Martinez, J. Guzmán, X. Zhang, and P. M. Jonas, “Parvalbumin+
interneurons obey unique connectivity rules and establish a powerful lateral-inhibition
microcircuit in dentate gyrus,” Nature Communications, vol. 9, no. 1. Nature
Publishing Group, 2018.
ista: Espinoza Martinez C, Guzmán J, Zhang X, Jonas PM. 2018. Parvalbumin+ interneurons
obey unique connectivity rules and establish a powerful lateral-inhibition microcircuit
in dentate gyrus. Nature Communications. 9(1), 4605.
mla: Espinoza Martinez, Claudia, et al. “Parvalbumin+ Interneurons Obey Unique Connectivity
Rules and Establish a Powerful Lateral-Inhibition Microcircuit in Dentate Gyrus.”
Nature Communications, vol. 9, no. 1, 4605, Nature Publishing Group, 2018,
doi:10.1038/s41467-018-06899-3.
short: C. Espinoza Martinez, J. Guzmán, X. Zhang, P.M. Jonas, Nature Communications
9 (2018).
date_created: 2018-12-11T11:44:12Z
date_published: 2018-11-02T00:00:00Z
date_updated: 2026-10-05T22:30:41Z
day: '02'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1038/s41467-018-06899-3
ec_funded: 1
external_id:
isi:
- '000449069700009'
file:
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checksum: 9fe2a63bd95a5067d896c087d07998f3
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isi: 1
issue: '1'
language:
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month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: Synaptic communication in neuronal microcircuits
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '8034'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/lateral-inhibition-keeps-similar-memories-apart/
record:
- id: '6363'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Parvalbumin+ interneurons obey unique connectivity rules and establish a powerful
lateral-inhibition microcircuit in dentate gyrus
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 9
year: '2018'
...
---
_id: '1117'
abstract:
- lang: eng
text: 'GABAergic synapses in brain circuits generate inhibitory output signals with
submillisecond latency and temporal precision. Whether the molecular identity
of the release sensor contributes to these signaling properties remains unclear.
Here, we examined the Ca^2+ sensor of exocytosis at GABAergic basket cell (BC)
to Purkinje cell (PC) synapses in cerebellum. Immunolabeling suggested that BC
terminals selectively expressed synaptotagmin 2 (Syt2), whereas synaptotagmin
1 (Syt1) was enriched in excitatory terminals. Genetic elimination of Syt2 reduced
action potential-evoked release to ∼10%, identifying Syt2 as the major Ca^2+ sensor
at BC-PC synapses. Differential adenovirus-mediated rescue revealed that Syt2
triggered release with shorter latency and higher temporal precision and mediated
faster vesicle pool replenishment than Syt1. Furthermore, deletion of Syt2 severely
reduced and delayed disynaptic inhibition following parallel fiber stimulation.
Thus, the selective use of Syt2 as release sensor at BC-PC synapses ensures fast
and efficient feedforward inhibition in cerebellar microcircuits. #bioimagingfacility-author'
acknowledged_ssus:
- _id: Bio
- _id: PreCl
article_processing_charge: No
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Itaru
full_name: Arai, Itaru
id: 32A73F6C-F248-11E8-B48F-1D18A9856A87
last_name: Arai
- first_name: Rachel
full_name: Satterield, Rachel
last_name: Satterield
- first_name: Samuel
full_name: Young, Samuel
last_name: Young
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Chen C, Arai itaru, Satterield R, Young S, Jonas PM. Synaptotagmin 2 is the
fast Ca2+ sensor at a central inhibitory synapse. Cell Reports. 2017;18(3):723-736.
doi:10.1016/j.celrep.2016.12.067
apa: Chen, C., Arai, itaru, Satterield, R., Young, S., & Jonas, P. M. (2017).
Synaptotagmin 2 is the fast Ca2+ sensor at a central inhibitory synapse. Cell
Reports. Cell Press. https://doi.org/10.1016/j.celrep.2016.12.067
chicago: Chen, Chong, itaru Arai, Rachel Satterield, Samuel Young, and Peter M Jonas.
“Synaptotagmin 2 Is the Fast Ca2+ Sensor at a Central Inhibitory Synapse.” Cell
Reports. Cell Press, 2017. https://doi.org/10.1016/j.celrep.2016.12.067.
ieee: C. Chen, itaru Arai, R. Satterield, S. Young, and P. M. Jonas, “Synaptotagmin
2 is the fast Ca2+ sensor at a central inhibitory synapse,” Cell Reports,
vol. 18, no. 3. Cell Press, pp. 723–736, 2017.
ista: Chen C, Arai itaru, Satterield R, Young S, Jonas PM. 2017. Synaptotagmin
2 is the fast Ca2+ sensor at a central inhibitory synapse. Cell Reports. 18(3),
723–736.
mla: Chen, Chong, et al. “Synaptotagmin 2 Is the Fast Ca2+ Sensor at a Central Inhibitory
Synapse.” Cell Reports, vol. 18, no. 3, Cell Press, 2017, pp. 723–36, doi:10.1016/j.celrep.2016.12.067.
short: C. Chen, itaru Arai, R. Satterield, S. Young, P.M. Jonas, Cell Reports 18
(2017) 723–736.
date_created: 2018-12-11T11:50:14Z
date_published: 2017-01-17T00:00:00Z
date_updated: 2026-04-08T14:09:28Z
day: '17'
ddc:
- '571'
department:
- _id: PeJo
doi: 10.1016/j.celrep.2016.12.067
ec_funded: 1
external_id:
isi:
- '000396470600013'
file:
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creator: system
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file_date_updated: 2018-12-12T10:16:09Z
fulldoi: https://doi.org/10.1016/j.celrep.2016.12.067
has_accepted_license: '1'
intvolume: ' 18'
isi: 1
issue: '3'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 723 - 736
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Cell Reports
publication_identifier:
issn:
- 2211-1247
publication_status: published
publisher: Cell Press
publist_id: '6245'
pubrep_id: '751'
quality_controlled: '1'
related_material:
record:
- id: '324'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Synaptotagmin 2 is the fast Ca2+ sensor at a central inhibitory synapse
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 18
year: '2017'
...
---
_id: '1118'
abstract:
- lang: eng
text: Sharp wave-ripple (SWR) oscillations play a key role in memory consolidation
during non-rapid eye movement sleep, immobility, and consummatory behavior. However,
whether temporally modulated synaptic excitation or inhibition underlies the ripples
is controversial. To address this question, we performed simultaneous recordings
of excitatory and inhibitory postsynaptic currents (EPSCs and IPSCs) and local
field potentials (LFPs) in the CA1 region of awake mice in vivo. During SWRs,
inhibition dominated over excitation, with a peak conductance ratio of 4.1 ± 0.5.
Furthermore, the amplitude of SWR-associated IPSCs was positively correlated with
SWR magnitude, whereas that of EPSCs was not. Finally, phase analysis indicated
that IPSCs were phase-locked to individual ripple cycles, whereas EPSCs were uniformly
distributed in phase space. Optogenetic inhibition indicated that PV+ interneurons
provided a major contribution to SWR-associated IPSCs. Thus, phasic inhibition,
but not excitation, shapes SWR oscillations in the hippocampal CA1 region in vivo.
acknowledged_ssus:
- _id: M-Shop
- _id: ScienComp
- _id: PreCl
article_processing_charge: No
author:
- first_name: Jian
full_name: Gan, Jian
id: 3614E438-F248-11E8-B48F-1D18A9856A87
last_name: Gan
- first_name: Shih-Ming
full_name: Weng, Shih-Ming
id: 2F9C5AC8-F248-11E8-B48F-1D18A9856A87
last_name: Weng
- first_name: Alejandro
full_name: Pernia-Andrade, Alejandro
id: 36963E98-F248-11E8-B48F-1D18A9856A87
last_name: Pernia-Andrade
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Gan J, Weng S-M, Pernia-Andrade A, Csicsvari JL, Jonas PM. Phase-locked inhibition,
but not excitation, underlies hippocampal ripple oscillations in awake mice in
vivo. Neuron. 2017;93(2):308-314. doi:10.1016/j.neuron.2016.12.018
apa: Gan, J., Weng, S.-M., Pernia-Andrade, A., Csicsvari, J. L., & Jonas, P.
M. (2017). Phase-locked inhibition, but not excitation, underlies hippocampal
ripple oscillations in awake mice in vivo. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2016.12.018
chicago: Gan, Jian, Shih-Ming Weng, Alejandro Pernia-Andrade, Jozsef L Csicsvari,
and Peter M Jonas. “Phase-Locked Inhibition, but Not Excitation, Underlies Hippocampal
Ripple Oscillations in Awake Mice in Vivo.” Neuron. Elsevier, 2017. https://doi.org/10.1016/j.neuron.2016.12.018.
ieee: J. Gan, S.-M. Weng, A. Pernia-Andrade, J. L. Csicsvari, and P. M. Jonas, “Phase-locked
inhibition, but not excitation, underlies hippocampal ripple oscillations in awake
mice in vivo,” Neuron, vol. 93, no. 2. Elsevier, pp. 308–314, 2017.
ista: Gan J, Weng S-M, Pernia-Andrade A, Csicsvari JL, Jonas PM. 2017. Phase-locked
inhibition, but not excitation, underlies hippocampal ripple oscillations in awake
mice in vivo. Neuron. 93(2), 308–314.
mla: Gan, Jian, et al. “Phase-Locked Inhibition, but Not Excitation, Underlies Hippocampal
Ripple Oscillations in Awake Mice in Vivo.” Neuron, vol. 93, no. 2, Elsevier,
2017, pp. 308–14, doi:10.1016/j.neuron.2016.12.018.
short: J. Gan, S.-M. Weng, A. Pernia-Andrade, J.L. Csicsvari, P.M. Jonas, Neuron
93 (2017) 308–314.
date_created: 2018-12-11T11:50:15Z
date_published: 2017-01-18T00:00:00Z
date_updated: 2025-04-15T07:20:01Z
day: '18'
ddc:
- '571'
department:
- _id: PeJo
- _id: JoCs
doi: 10.1016/j.neuron.2016.12.018
ec_funded: 1
external_id:
isi:
- '000396428200010'
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:08:56Z
date_updated: 2018-12-12T10:08:56Z
file_id: '4719'
file_name: IST-2017-752-v1+1_1-s2.0-S0896627316309606-main.pdf
file_size: 2738950
relation: main_file
file_date_updated: 2018-12-12T10:08:56Z
fulldoi: https://doi.org/10.1016/j.neuron.2016.12.018
has_accepted_license: '1'
intvolume: ' 93'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 308 - 314
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Neuron
publication_status: published
publisher: Elsevier
publist_id: '6244'
pubrep_id: '752'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Phase-locked inhibition, but not excitation, underlies hippocampal ripple oscillations
in awake mice in vivo
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 93
year: '2017'
...
---
_id: '630'
abstract:
- lang: eng
text: 'Background: Standards have become available to share semantically encoded
vital parameters from medical devices, as required for example by personal healthcare
records. Standardised sharing of biosignal data largely remains open. Objectives:
The goal of this work is to explore available biosignal file format and data exchange
standards and profiles, and to conceptualise end-To-end solutions. Methods: The
authors reviewed and discussed available biosignal file format standards with
other members of international standards development organisations (SDOs). Results:
A raw concept for standards based acquisition, storage, archiving and sharing
of biosignals was developed. The GDF format may serve for storing biosignals.
Signals can then be shared using FHIR resources and may be stored on FHIR servers
or in DICOM archives, with DICOM waveforms as one possible format. Conclusion:
Currently a group of international SDOs (e.g. HL7, IHE, DICOM, IEEE) is engaged
in intensive discussions. This discussion extends existing work that already was
adopted by large implementer communities. The concept presented here only reports
the current status of the discussion in Austria. The discussion will continue
internationally, with results to be expected over the coming years.'
alternative_title:
- Studies in Health Technology and Informatics
article_processing_charge: No
author:
- first_name: Stefan
full_name: Sauermann, Stefan
last_name: Sauermann
- first_name: Veronika
full_name: David, Veronika
last_name: David
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Reinhard
full_name: Egelkraut, Reinhard
last_name: Egelkraut
- first_name: Matthias
full_name: Frohner, Matthias
last_name: Frohner
- first_name: Birgit
full_name: Pohn, Birgit
last_name: Pohn
- first_name: Philipp
full_name: Urbauer, Philipp
last_name: Urbauer
- first_name: Alexander
full_name: Mense, Alexander
last_name: Mense
citation:
ama: 'Sauermann S, David V, Schlögl A, et al. Biosignals standards and FHIR: The
way to go. In: Vol 236. IOS Press; 2017:356-362. doi:10.3233/978-1-61499-759-7-356'
apa: 'Sauermann, S., David, V., Schlögl, A., Egelkraut, R., Frohner, M., Pohn, B.,
… Mense, A. (2017). Biosignals standards and FHIR: The way to go (Vol. 236, pp.
356–362). Presented at the eHealth: Health Informatics Meets eHealth, Vienna,
Austria: IOS Press. https://doi.org/10.3233/978-1-61499-759-7-356'
chicago: 'Sauermann, Stefan, Veronika David, Alois Schlögl, Reinhard Egelkraut,
Matthias Frohner, Birgit Pohn, Philipp Urbauer, and Alexander Mense. “Biosignals
Standards and FHIR: The Way to Go,” 236:356–62. IOS Press, 2017. https://doi.org/10.3233/978-1-61499-759-7-356.'
ieee: 'S. Sauermann et al., “Biosignals standards and FHIR: The way to go,”
presented at the eHealth: Health Informatics Meets eHealth, Vienna, Austria, 2017,
vol. 236, pp. 356–362.'
ista: 'Sauermann S, David V, Schlögl A, Egelkraut R, Frohner M, Pohn B, Urbauer
P, Mense A. 2017. Biosignals standards and FHIR: The way to go. eHealth: Health
Informatics Meets eHealth, Studies in Health Technology and Informatics, vol.
236, 356–362.'
mla: 'Sauermann, Stefan, et al. Biosignals Standards and FHIR: The Way to Go.
Vol. 236, IOS Press, 2017, pp. 356–62, doi:10.3233/978-1-61499-759-7-356.'
short: S. Sauermann, V. David, A. Schlögl, R. Egelkraut, M. Frohner, B. Pohn, P.
Urbauer, A. Mense, in:, IOS Press, 2017, pp. 356–362.
conference:
end_date: 2017-05-24
location: Vienna, Austria
name: 'eHealth: Health Informatics Meets eHealth'
start_date: 2017-05-23
date_created: 2018-12-11T11:47:36Z
date_published: 2017-01-01T00:00:00Z
date_updated: 2025-09-11T07:27:53Z
day: '01'
ddc:
- '005'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.3233/978-1-61499-759-7-356
external_id:
isi:
- '000426828000047'
file:
- access_level: open_access
checksum: 1254dcc5b04a996d97fad9a726b42727
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:56Z
date_updated: 2020-07-14T12:47:27Z
file_id: '4913'
file_name: IST-2017-906-v1+1_SHTI236-0356.pdf
file_size: 443635
relation: main_file
file_date_updated: 2020-07-14T12:47:27Z
fulldoi: https://doi.org/10.3233/978-1-61499-759-7-356
has_accepted_license: '1'
intvolume: ' 236'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '01'
oa: 1
oa_version: Published Version
page: 356 - 362
publication_identifier:
isbn:
- 978-161499758-0
publication_status: published
publisher: IOS Press
publist_id: '7164'
pubrep_id: '906'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Biosignals standards and FHIR: The way to go'
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: conference
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 236
year: '2017'
...
---
_id: '800'
abstract:
- lang: eng
text: Gamma oscillations (30–150 Hz) in neuronal networks are associated with the
processing and recall of information. We measured local field potentials in the
dentate gyrus of freely moving mice and found that gamma activity occurs in bursts,
which are highly heterogeneous in their spatial extensions, ranging from focal
to global coherent events. Synaptic communication among perisomatic-inhibitory
interneurons (PIIs) is thought to play an important role in the generation of
hippocampal gamma patterns. However, how neuronal circuits can generate synchronous
oscillations at different spatial scales is unknown. We analyzed paired recordings
in dentate gyrus slices and show that synaptic signaling at interneuron-interneuron
synapses is distance dependent. Synaptic strength declines whereas the duration
of inhibitory signals increases with axonal distance among interconnected PIIs.
Using neuronal network modeling, we show that distance-dependent inhibition generates
multiple highly synchronous focal gamma bursts allowing the network to process
complex inputs in parallel in flexibly organized neuronal centers.
article_number: '758'
article_processing_charge: No
author:
- first_name: Michael
full_name: Strüber, Michael
last_name: Strüber
- first_name: Jonas
full_name: Sauer, Jonas
last_name: Sauer
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Marlene
full_name: Bartos, Marlene
last_name: Bartos
citation:
ama: Strüber M, Sauer J, Jonas PM, Bartos M. Distance-dependent inhibition facilitates
focality of gamma oscillations in the dentate gyrus. Nature Communications.
2017;8(1). doi:10.1038/s41467-017-00936-3
apa: Strüber, M., Sauer, J., Jonas, P. M., & Bartos, M. (2017). Distance-dependent
inhibition facilitates focality of gamma oscillations in the dentate gyrus. Nature
Communications. Nature Publishing Group. https://doi.org/10.1038/s41467-017-00936-3
chicago: Strüber, Michael, Jonas Sauer, Peter M Jonas, and Marlene Bartos. “Distance-Dependent
Inhibition Facilitates Focality of Gamma Oscillations in the Dentate Gyrus.” Nature
Communications. Nature Publishing Group, 2017. https://doi.org/10.1038/s41467-017-00936-3.
ieee: M. Strüber, J. Sauer, P. M. Jonas, and M. Bartos, “Distance-dependent inhibition
facilitates focality of gamma oscillations in the dentate gyrus,” Nature Communications,
vol. 8, no. 1. Nature Publishing Group, 2017.
ista: Strüber M, Sauer J, Jonas PM, Bartos M. 2017. Distance-dependent inhibition
facilitates focality of gamma oscillations in the dentate gyrus. Nature Communications.
8(1), 758.
mla: Strüber, Michael, et al. “Distance-Dependent Inhibition Facilitates Focality
of Gamma Oscillations in the Dentate Gyrus.” Nature Communications, vol.
8, no. 1, 758, Nature Publishing Group, 2017, doi:10.1038/s41467-017-00936-3.
short: M. Strüber, J. Sauer, P.M. Jonas, M. Bartos, Nature Communications 8 (2017).
date_created: 2018-12-11T11:48:34Z
date_published: 2017-10-02T00:00:00Z
date_updated: 2025-07-10T11:54:59Z
day: '02'
ddc:
- '571'
department:
- _id: PeJo
doi: 10.1038/s41467-017-00936-3
ec_funded: 1
external_id:
isi:
- '000412053100004'
file:
- access_level: open_access
checksum: 7e2c7621afd5f802338e92e8619f024d
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:15:17Z
date_updated: 2020-07-14T12:48:07Z
file_id: '5135'
file_name: IST-2017-914-v1+1_s41467-017-00936-3.pdf
file_size: 4261832
relation: main_file
file_date_updated: 2020-07-14T12:48:07Z
fulldoi: https://doi.org/10.1038/s41467-017-00936-3
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '1'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Nature Publishing Group
publist_id: '6853'
pubrep_id: '914'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distance-dependent inhibition facilitates focality of gamma oscillations in
the dentate gyrus
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2017'
...
---
_id: '706'
abstract:
- lang: eng
text: A hippocampal mossy fiber synapse has a complex structure and is implicated
in learning and memory. In this synapse, the mossy fiber boutons attach to the
dendritic shaft by puncta adherentia junctions and wrap around a multiply-branched
spine, forming synaptic junctions. We have recently shown using transmission electron
microscopy, immunoelectron microscopy and serial block face-scanning electron
microscopy that atypical puncta adherentia junctions are formed in the afadin-deficient
mossy fiber synapse and that the complexity of postsynaptic spines and mossy fiber
boutons, the number of spine heads, the area of postsynaptic densities and the
density of synaptic vesicles docked to active zones are decreased in the afadin-deficient
synapse. We investigated here the roles of afadin in the functional differentiations
of the mossy fiber synapse using the afadin-deficient mice. The electrophysiological
studies showed that both the release probability of glutamate and the postsynaptic
responsiveness to glutamate were markedly reduced, but not completely lost, in
the afadin-deficient mossy fiber synapse, whereas neither long-term potentiation
nor long-term depression was affected. These results indicate that afadin plays
roles in the functional differentiations of the presynapse and the postsynapse
of the hippocampal mossy fiber synapse.
article_processing_charge: No
author:
- first_name: Xiaoqi
full_name: Geng, Xiaoqi
id: 3395256A-F248-11E8-B48F-1D18A9856A87
last_name: Geng
- first_name: Tomohiko
full_name: Maruo, Tomohiko
last_name: Maruo
- first_name: Kenji
full_name: Mandai, Kenji
last_name: Mandai
- first_name: Irwan
full_name: Supriyanto, Irwan
last_name: Supriyanto
- first_name: Muneaki
full_name: Miyata, Muneaki
last_name: Miyata
- first_name: Shotaro
full_name: Sakakibara, Shotaro
last_name: Sakakibara
- first_name: Akira
full_name: Mizoguchi, Akira
last_name: Mizoguchi
- first_name: Yoshimi
full_name: Takai, Yoshimi
last_name: Takai
- first_name: Masahiro
full_name: Mori, Masahiro
last_name: Mori
citation:
ama: Geng X, Maruo T, Mandai K, et al. Roles of afadin in functional differentiations
of hippocampal mossy fiber synapse. Genes to Cells. 2017;22(8):715-722.
doi:10.1111/gtc.12508
apa: Geng, X., Maruo, T., Mandai, K., Supriyanto, I., Miyata, M., Sakakibara, S.,
… Mori, M. (2017). Roles of afadin in functional differentiations of hippocampal
mossy fiber synapse. Genes to Cells. Wiley-Blackwell. https://doi.org/10.1111/gtc.12508
chicago: Geng, Xiaoqi, Tomohiko Maruo, Kenji Mandai, Irwan Supriyanto, Muneaki Miyata,
Shotaro Sakakibara, Akira Mizoguchi, Yoshimi Takai, and Masahiro Mori. “Roles
of Afadin in Functional Differentiations of Hippocampal Mossy Fiber Synapse.”
Genes to Cells. Wiley-Blackwell, 2017. https://doi.org/10.1111/gtc.12508.
ieee: X. Geng et al., “Roles of afadin in functional differentiations of
hippocampal mossy fiber synapse,” Genes to Cells, vol. 22, no. 8. Wiley-Blackwell,
pp. 715–722, 2017.
ista: Geng X, Maruo T, Mandai K, Supriyanto I, Miyata M, Sakakibara S, Mizoguchi
A, Takai Y, Mori M. 2017. Roles of afadin in functional differentiations of hippocampal
mossy fiber synapse. Genes to Cells. 22(8), 715–722.
mla: Geng, Xiaoqi, et al. “Roles of Afadin in Functional Differentiations of Hippocampal
Mossy Fiber Synapse.” Genes to Cells, vol. 22, no. 8, Wiley-Blackwell,
2017, pp. 715–22, doi:10.1111/gtc.12508.
short: X. Geng, T. Maruo, K. Mandai, I. Supriyanto, M. Miyata, S. Sakakibara, A.
Mizoguchi, Y. Takai, M. Mori, Genes to Cells 22 (2017) 715–722.
date_created: 2018-12-11T11:48:02Z
date_published: 2017-08-01T00:00:00Z
date_updated: 2025-09-10T11:06:14Z
day: '01'
department:
- _id: PeJo
doi: 10.1111/gtc.12508
external_id:
isi:
- '000409224300003'
fulldoi: https://doi.org/10.1111/gtc.12508
intvolume: ' 22'
isi: 1
issue: '8'
language:
- iso: eng
month: '08'
oa_version: None
page: 715 - 722
publication: Genes to Cells
publication_identifier:
issn:
- 1356-9597
publication_status: published
publisher: Wiley-Blackwell
publist_id: '6987'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Roles of afadin in functional differentiations of hippocampal mossy fiber synapse
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 22
year: '2017'
...
---
_id: '749'
abstract:
- lang: eng
text: 'Synaptotagmin 7 (Syt7) is thought to be a Ca2+ sensor that mediates asynchronous
transmitter release and facilitation at synapses. However, Syt7 is strongly expressed
in fast-spiking, parvalbumin-expressing GABAergic interneurons, and the output
synapses of these neurons produce only minimal asynchronous release and show depression
rather than facilitation. To resolve this apparent contradiction, we examined
the effects of genetic elimination of Syt7 on synaptic transmission at the GABAergic
basket cell (BC)-Purkinje cell (PC) synapse in cerebellum. Our results indicate
that at the BC-PC synapse, Syt7 contributes to asynchronous release, pool replenishment,
and facilitation. In combination, these three effects ensure efficient transmitter
release during high-frequency activity and guarantee frequency independence of
inhibition. Our results identify a distinct function of Syt7: ensuring the efficiency
of high-frequency inhibitory synaptic transmission'
acknowledged_ssus:
- _id: PreCl
article_processing_charge: No
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Rachel
full_name: Satterfield, Rachel
last_name: Satterfield
- first_name: Samuel
full_name: Young, Samuel
last_name: Young
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Chen C, Satterfield R, Young S, Jonas PM. Triple function of Synaptotagmin
7 ensures efficiency of high-frequency transmission at central GABAergic synapses.
Cell Reports. 2017;21(8):2082-2089. doi:10.1016/j.celrep.2017.10.122
apa: Chen, C., Satterfield, R., Young, S., & Jonas, P. M. (2017). Triple function
of Synaptotagmin 7 ensures efficiency of high-frequency transmission at central
GABAergic synapses. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2017.10.122
chicago: Chen, Chong, Rachel Satterfield, Samuel Young, and Peter M Jonas. “Triple
Function of Synaptotagmin 7 Ensures Efficiency of High-Frequency Transmission
at Central GABAergic Synapses.” Cell Reports. Cell Press, 2017. https://doi.org/10.1016/j.celrep.2017.10.122.
ieee: C. Chen, R. Satterfield, S. Young, and P. M. Jonas, “Triple function of Synaptotagmin
7 ensures efficiency of high-frequency transmission at central GABAergic synapses,”
Cell Reports, vol. 21, no. 8. Cell Press, pp. 2082–2089, 2017.
ista: Chen C, Satterfield R, Young S, Jonas PM. 2017. Triple function of Synaptotagmin
7 ensures efficiency of high-frequency transmission at central GABAergic synapses.
Cell Reports. 21(8), 2082–2089.
mla: Chen, Chong, et al. “Triple Function of Synaptotagmin 7 Ensures Efficiency
of High-Frequency Transmission at Central GABAergic Synapses.” Cell Reports,
vol. 21, no. 8, Cell Press, 2017, pp. 2082–89, doi:10.1016/j.celrep.2017.10.122.
short: C. Chen, R. Satterfield, S. Young, P.M. Jonas, Cell Reports 21 (2017) 2082–2089.
corr_author: '1'
date_created: 2018-12-11T11:48:18Z
date_published: 2017-11-21T00:00:00Z
date_updated: 2026-04-08T14:09:28Z
day: '21'
ddc:
- '570'
- '571'
department:
- _id: PeJo
doi: 10.1016/j.celrep.2017.10.122
ec_funded: 1
external_id:
isi:
- '000416216700007'
file:
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checksum: a6afa3764909bf6edafa07982d8e1cee
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:14Z
date_updated: 2020-07-14T12:47:59Z
file_id: '4737'
file_name: IST-2017-874-v1+1_PIIS2211124717316029.pdf
file_size: 2759195
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
fulldoi: https://doi.org/10.1016/j.celrep.2017.10.122
has_accepted_license: '1'
intvolume: ' 21'
isi: 1
issue: '8'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 2082 - 2089
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
publication: Cell Reports
publication_identifier:
issn:
- 2211-1247
publication_status: published
publisher: Cell Press
publist_id: '6907'
pubrep_id: '874'
quality_controlled: '1'
related_material:
record:
- id: '324'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Triple function of Synaptotagmin 7 ensures efficiency of high-frequency transmission
at central GABAergic synapses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 21
year: '2017'
...
---
_id: '991'
abstract:
- lang: eng
text: Synaptotagmin 7 (Syt7) was originally identified as a slow Ca2+ sensor for
lysosome fusion, but its function at fast synapses is controversial. The paper
by Luo and Südhof (2017) in this issue of Neuron shows that at the calyx of Held
in the auditory brainstem Syt7 triggers asynchronous release during stimulus trains,
resulting in reliable and temporally precise high-frequency transmission. Thus,
a slow Ca2+ sensor contributes to the fast signaling properties of the calyx synapse.
article_processing_charge: No
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Chen C, Jonas PM. Synaptotagmins: That’s why so many. Neuron. 2017;94(4):694-696.
doi:10.1016/j.neuron.2017.05.011'
apa: 'Chen, C., & Jonas, P. M. (2017). Synaptotagmins: That’s why so many. Neuron.
Elsevier. https://doi.org/10.1016/j.neuron.2017.05.011'
chicago: 'Chen, Chong, and Peter M Jonas. “Synaptotagmins: That’s Why so Many.”
Neuron. Elsevier, 2017. https://doi.org/10.1016/j.neuron.2017.05.011.'
ieee: 'C. Chen and P. M. Jonas, “Synaptotagmins: That’s why so many,” Neuron,
vol. 94, no. 4. Elsevier, pp. 694–696, 2017.'
ista: 'Chen C, Jonas PM. 2017. Synaptotagmins: That’s why so many. Neuron. 94(4),
694–696.'
mla: 'Chen, Chong, and Peter M. Jonas. “Synaptotagmins: That’s Why so Many.” Neuron,
vol. 94, no. 4, Elsevier, 2017, pp. 694–96, doi:10.1016/j.neuron.2017.05.011.'
short: C. Chen, P.M. Jonas, Neuron 94 (2017) 694–696.
date_created: 2018-12-11T11:49:34Z
date_published: 2017-05-17T00:00:00Z
date_updated: 2026-04-16T10:05:51Z
day: '17'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2017.05.011
external_id:
isi:
- '000401415100002'
fulldoi: https://doi.org/10.1016/j.neuron.2017.05.011
intvolume: ' 94'
isi: 1
issue: '4'
language:
- iso: eng
month: '05'
oa_version: None
page: 694 - 696
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
publist_id: '6408'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Synaptotagmins: That’s why so many'
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 94
year: '2017'
...
---
_id: '1142'
abstract:
- lang: eng
text: Hemolysis drives susceptibility to bacterial infections and predicts poor
outcome from sepsis. These detrimental effects are commonly considered to be a
consequence of heme-iron serving as a nutrient for bacteria. We employed a Gram-negative
sepsis model and found that elevated heme levels impaired the control of bacterial
proliferation independently of heme-iron acquisition by pathogens. Heme strongly
inhibited phagocytosis and the migration of human and mouse phagocytes by disrupting
actin cytoskeletal dynamics via activation of the GTP-binding Rho family protein
Cdc42 by the guanine nucleotide exchange factor DOCK8. A chemical screening approach
revealed that quinine effectively prevented heme effects on the cytoskeleton,
restored phagocytosis and improved survival in sepsis. These mechanistic insights
provide potential therapeutic targets for patients with sepsis or hemolytic disorders.
acknowledgement: 'Y. Fukui (Medical Institute of Bioregulation, Kyushu University)
and J. Stein (Theodor Kocher Institute, University of Bern) are acknowledged for
providing the DOCK8 deficient bone marrow. and H. Häcker (St. Judes Children''s
Research Hospital) for providing the ERHBD-HoxB8-encoding retroviral construct.
pSpCas9(BB)-2a-Puro (PX459) was a gift from F. Zhang (Massachusetts Institute of
Technology) (Addgene plasmid # 48139) and pGRG36 was a gift from N. Craig (Johns
Hopkins University School of Medicine) (Addgene plasmid # 16666). LifeAct-GFP-encoding
retrovirus was kindly provided by A. Leithner (Institute of Science and Technology
Austria). pSIM8 and TKC E. coli were gifts from D.L. Court (Center for Cancer Research,
National Cancer Institute). We acknowledge M. Gröger and S. Rauscher for excellent
technical support (Core imaging facility, Medical University of Vienna). We thank
D.P. Barlow and L.R. Cheever for critical reading of the manuscript. This work was
supported by the Austrian Academy of Sciences, the Science Fund of the Austrian
National Bank (14107) and the Austrian Science Fund FWF (I1620-B22) in the Infect-ERA
framework (to S.Knapp).'
article_processing_charge: No
author:
- first_name: Rui
full_name: Martins, Rui
last_name: Martins
- first_name: Julia
full_name: Maier, Julia
last_name: Maier
- first_name: Anna
full_name: Gorki, Anna
last_name: Gorki
- first_name: Kilian
full_name: Huber, Kilian
last_name: Huber
- first_name: Omar
full_name: Sharif, Omar
last_name: Sharif
- first_name: Philipp
full_name: Starkl, Philipp
last_name: Starkl
- first_name: Simona
full_name: Saluzzo, Simona
last_name: Saluzzo
- first_name: Federica
full_name: Quattrone, Federica
last_name: Quattrone
- first_name: Riem
full_name: Gawish, Riem
last_name: Gawish
- first_name: Karin
full_name: Lakovits, Karin
last_name: Lakovits
- first_name: Michael
full_name: Aichinger, Michael
last_name: Aichinger
- first_name: Branka
full_name: Radic Sarikas, Branka
last_name: Radic Sarikas
- first_name: Charles
full_name: Lardeau, Charles
last_name: Lardeau
- first_name: Anastasiya
full_name: Hladik, Anastasiya
last_name: Hladik
- first_name: Ana
full_name: Korosec, Ana
last_name: Korosec
- first_name: Markus
full_name: Brown, Markus
id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
last_name: Brown
- first_name: Kari
full_name: Vaahtomeri, Kari
id: 368EE576-F248-11E8-B48F-1D18A9856A87
last_name: Vaahtomeri
orcid: 0000-0001-7829-3518
- first_name: Michelle
full_name: Duggan, Michelle
id: 2EDEA62C-F248-11E8-B48F-1D18A9856A87
last_name: Duggan
- first_name: Dontscho
full_name: Kerjaschki, Dontscho
last_name: Kerjaschki
- first_name: Harald
full_name: Esterbauer, Harald
last_name: Esterbauer
- first_name: Jacques
full_name: Colinge, Jacques
last_name: Colinge
- first_name: Stephanie
full_name: Eisenbarth, Stephanie
last_name: Eisenbarth
- first_name: Thomas
full_name: Decker, Thomas
last_name: Decker
- first_name: Keiryn
full_name: Bennett, Keiryn
last_name: Bennett
- first_name: Stefan
full_name: Kubicek, Stefan
last_name: Kubicek
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Giulio
full_name: Superti Furga, Giulio
last_name: Superti Furga
- first_name: Sylvia
full_name: Knapp, Sylvia
last_name: Knapp
citation:
ama: Martins R, Maier J, Gorki A, et al. Heme drives hemolysis-induced susceptibility
to infection via disruption of phagocyte functions. Nature Immunology.
2016;17(12):1361-1372. doi:10.1038/ni.3590
apa: Martins, R., Maier, J., Gorki, A., Huber, K., Sharif, O., Starkl, P., … Knapp,
S. (2016). Heme drives hemolysis-induced susceptibility to infection via disruption
of phagocyte functions. Nature Immunology. Nature Publishing Group. https://doi.org/10.1038/ni.3590
chicago: Martins, Rui, Julia Maier, Anna Gorki, Kilian Huber, Omar Sharif, Philipp
Starkl, Simona Saluzzo, et al. “Heme Drives Hemolysis-Induced Susceptibility to
Infection via Disruption of Phagocyte Functions.” Nature Immunology. Nature
Publishing Group, 2016. https://doi.org/10.1038/ni.3590.
ieee: R. Martins et al., “Heme drives hemolysis-induced susceptibility to
infection via disruption of phagocyte functions,” Nature Immunology, vol.
17, no. 12. Nature Publishing Group, pp. 1361–1372, 2016.
ista: Martins R, Maier J, Gorki A, Huber K, Sharif O, Starkl P, Saluzzo S, Quattrone
F, Gawish R, Lakovits K, Aichinger M, Radic Sarikas B, Lardeau C, Hladik A, Korosec
A, Brown M, Vaahtomeri K, Duggan M, Kerjaschki D, Esterbauer H, Colinge J, Eisenbarth
S, Decker T, Bennett K, Kubicek S, Sixt MK, Superti Furga G, Knapp S. 2016. Heme
drives hemolysis-induced susceptibility to infection via disruption of phagocyte
functions. Nature Immunology. 17(12), 1361–1372.
mla: Martins, Rui, et al. “Heme Drives Hemolysis-Induced Susceptibility to Infection
via Disruption of Phagocyte Functions.” Nature Immunology, vol. 17, no.
12, Nature Publishing Group, 2016, pp. 1361–72, doi:10.1038/ni.3590.
short: R. Martins, J. Maier, A. Gorki, K. Huber, O. Sharif, P. Starkl, S. Saluzzo,
F. Quattrone, R. Gawish, K. Lakovits, M. Aichinger, B. Radic Sarikas, C. Lardeau,
A. Hladik, A. Korosec, M. Brown, K. Vaahtomeri, M. Duggan, D. Kerjaschki, H. Esterbauer,
J. Colinge, S. Eisenbarth, T. Decker, K. Bennett, S. Kubicek, M.K. Sixt, G. Superti
Furga, S. Knapp, Nature Immunology 17 (2016) 1361–1372.
date_created: 2018-12-11T11:50:22Z
date_published: 2016-12-01T00:00:00Z
date_updated: 2025-09-22T14:10:50Z
day: '01'
department:
- _id: MiSi
- _id: PeJo
doi: 10.1038/ni.3590
external_id:
isi:
- '000388056400006'
fulldoi: https://doi.org/10.1038/ni.3590
intvolume: ' 17'
isi: 1
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://ora.ox.ac.uk/objects/uuid:f53a464e-1e5b-4f08-a7d8-b6749b852b9d
month: '12'
oa: 1
oa_version: Submitted Version
page: 1361 - 1372
publication: Nature Immunology
publication_status: published
publisher: Nature Publishing Group
publist_id: '6216'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Heme drives hemolysis-induced susceptibility to infection via disruption of
phagocyte functions
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 17
year: '2016'
...
---
_id: '12903'
article_processing_charge: No
author:
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Stephan
full_name: Stadlbauer, Stephan
id: 4D0BC184-F248-11E8-B48F-1D18A9856A87
last_name: Stadlbauer
citation:
ama: 'Schlögl A, Stadlbauer S. High performance computing at IST Austria: Modelling
the human hippocampus. In: AHPC16 - Austrian HPC Meeting 2016. VSC - Vienna
Scientific Cluster; 2016:37.'
apa: 'Schlögl, A., & Stadlbauer, S. (2016). High performance computing at IST
Austria: Modelling the human hippocampus. In AHPC16 - Austrian HPC Meeting
2016 (p. 37). Grundlsee, Austria: VSC - Vienna Scientific Cluster.'
chicago: 'Schlögl, Alois, and Stephan Stadlbauer. “High Performance Computing at
IST Austria: Modelling the Human Hippocampus.” In AHPC16 - Austrian HPC Meeting
2016, 37. VSC - Vienna Scientific Cluster, 2016.'
ieee: 'A. Schlögl and S. Stadlbauer, “High performance computing at IST Austria:
Modelling the human hippocampus,” in AHPC16 - Austrian HPC Meeting 2016,
Grundlsee, Austria, 2016, p. 37.'
ista: 'Schlögl A, Stadlbauer S. 2016. High performance computing at IST Austria:
Modelling the human hippocampus. AHPC16 - Austrian HPC Meeting 2016. AHPC: Austrian
HPC Meeting, 37.'
mla: 'Schlögl, Alois, and Stephan Stadlbauer. “High Performance Computing at IST
Austria: Modelling the Human Hippocampus.” AHPC16 - Austrian HPC Meeting 2016,
VSC - Vienna Scientific Cluster, 2016, p. 37.'
short: A. Schlögl, S. Stadlbauer, in:, AHPC16 - Austrian HPC Meeting 2016, VSC -
Vienna Scientific Cluster, 2016, p. 37.
conference:
end_date: 2016-02-24
location: Grundlsee, Austria
name: 'AHPC: Austrian HPC Meeting'
start_date: 2016-02-22
corr_author: '1'
date_created: 2023-05-05T12:54:47Z
date_published: 2016-02-24T00:00:00Z
date_updated: 2024-10-09T21:05:23Z
day: '24'
ddc:
- '000'
department:
- _id: ScienComp
- _id: PeJo
file:
- access_level: open_access
checksum: 4a7b00362e81358d568f5e216fa03c3e
content_type: application/pdf
creator: dernst
date_created: 2023-05-16T07:03:56Z
date_updated: 2023-05-16T07:03:56Z
file_id: '12968'
file_name: 2016_AHPC_Schloegl.pdf
file_size: 1073523
relation: main_file
success: 1
file_date_updated: 2023-05-16T07:03:56Z
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://vsc.ac.at/fileadmin/user_upload/vsc/conferences/ahpc16/BOOKLET_AHPC16.pdf
month: '02'
oa: 1
oa_version: Published Version
page: '37'
publication: AHPC16 - Austrian HPC Meeting 2016
publication_status: published
publisher: VSC - Vienna Scientific Cluster
quality_controlled: '1'
status: public
title: 'High performance computing at IST Austria: Modelling the human hippocampus'
type: conference_abstract
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2016'
...
---
_id: '1323'
abstract:
- lang: eng
text: Mossy fiber synapses on CA3 pyramidal cells are 'conditional detonators' that
reliably discharge postsynaptic targets. The 'conditional' nature implies that
burst activity in dentate gyrus granule cells is required for detonation. Whether
single unitary excitatory postsynaptic potentials (EPSPs) trigger spikes in CA3
neurons remains unknown. Mossy fiber synapses exhibit both pronounced short-term
facilitation and uniquely large post-tetanic potentiation (PTP). We tested whether
PTP could convert mossy fiber synapses from subdetonator into detonator mode,
using a recently developed method to selectively and noninvasively stimulate individual
presynaptic terminals in rat brain slices. Unitary EPSPs failed to initiate a
spike in CA3 neurons under control conditions, but reliably discharged them after
induction of presynaptic short-term plasticity. Remarkably, PTP switched mossy
fiber synapses into full detonators for tens of seconds. Plasticity-dependent
detonation may be critical for efficient coding, storage, and recall of information
in the granule cell–CA3 cell network.
acknowledged_ssus:
- _id: M-Shop
- _id: PreCl
article_number: e17977
article_processing_charge: No
author:
- first_name: Nicholas
full_name: Vyleta, Nicholas
id: 36C4978E-F248-11E8-B48F-1D18A9856A87
last_name: Vyleta
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Vyleta N, Borges Merjane C, Jonas PM. Plasticity-dependent, full detonation
at hippocampal mossy fiber–CA3 pyramidal neuron synapses. eLife. 2016;5.
doi:10.7554/eLife.17977
apa: Vyleta, N., Borges Merjane, C., & Jonas, P. M. (2016). Plasticity-dependent,
full detonation at hippocampal mossy fiber–CA3 pyramidal neuron synapses. ELife.
eLife Sciences Publications. https://doi.org/10.7554/eLife.17977
chicago: Vyleta, Nicholas, Carolina Borges Merjane, and Peter M Jonas. “Plasticity-Dependent,
Full Detonation at Hippocampal Mossy Fiber–CA3 Pyramidal Neuron Synapses.” ELife.
eLife Sciences Publications, 2016. https://doi.org/10.7554/eLife.17977.
ieee: N. Vyleta, C. Borges Merjane, and P. M. Jonas, “Plasticity-dependent, full
detonation at hippocampal mossy fiber–CA3 pyramidal neuron synapses,” eLife,
vol. 5. eLife Sciences Publications, 2016.
ista: Vyleta N, Borges Merjane C, Jonas PM. 2016. Plasticity-dependent, full detonation
at hippocampal mossy fiber–CA3 pyramidal neuron synapses. eLife. 5, e17977.
mla: Vyleta, Nicholas, et al. “Plasticity-Dependent, Full Detonation at Hippocampal
Mossy Fiber–CA3 Pyramidal Neuron Synapses.” ELife, vol. 5, e17977, eLife
Sciences Publications, 2016, doi:10.7554/eLife.17977.
short: N. Vyleta, C. Borges Merjane, P.M. Jonas, ELife 5 (2016).
corr_author: '1'
date_created: 2018-12-11T11:51:22Z
date_published: 2016-10-25T00:00:00Z
date_updated: 2025-09-22T08:26:29Z
day: '25'
ddc:
- '571'
- '572'
department:
- _id: PeJo
doi: 10.7554/eLife.17977
ec_funded: 1
external_id:
isi:
- '000387034600001'
file:
- access_level: open_access
checksum: a7201280c571bed88ebd459ce5ce6a47
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:05Z
date_updated: 2020-07-14T12:44:44Z
file_id: '5257'
file_name: IST-2016-715-v1+1_e17977-download.pdf
file_size: 1477891
relation: main_file
file_date_updated: 2020-07-14T12:44:44Z
fulldoi: https://doi.org/10.7554/eLife.17977
has_accepted_license: '1'
intvolume: ' 5'
isi: 1
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glutamatergic synapse
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '5947'
pubrep_id: '715'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Plasticity-dependent, full detonation at hippocampal mossy fiber–CA3 pyramidal
neuron synapses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 5
year: '2016'
...
---
_id: '1350'
abstract:
- lang: eng
text: "The hippocampal CA3 region plays a key role in learning and memory. Recurrent
CA3–CA3\r\nsynapses are thought to be the subcellular substrate of pattern completion.
However, the\r\nsynaptic mechanisms of this network computation remain enigmatic.
To investigate these mechanisms, we combined functional connectivity analysis
with network modeling.\r\nSimultaneous recording fromup to eight CA3 pyramidal
neurons revealed that connectivity was sparse, spatially uniform, and highly enriched
in disynaptic motifs (reciprocal, convergence,divergence, and chain motifs). Unitary
connections were composed of one or two synaptic contacts, suggesting efficient
use of postsynaptic space. Real-size modeling indicated that CA3 networks with
sparse connectivity, disynaptic motifs, and single-contact connections robustly
generated pattern completion.Thus, macro- and microconnectivity contribute to
efficient\r\nmemory storage and retrieval in hippocampal networks."
acknowledged_ssus:
- _id: ScienComp
article_processing_charge: No
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
orcid: 0000-0003-2209-5242
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Michael
full_name: Frotscher, Michael
last_name: Frotscher
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Guzmán J, Schlögl A, Frotscher M, Jonas PM. Synaptic mechanisms of pattern
completion in the hippocampal CA3 network. Science. 2016;353(6304):1117-1123.
doi:10.1126/science.aaf1836
apa: Guzmán, J., Schlögl, A., Frotscher, M., & Jonas, P. M. (2016). Synaptic
mechanisms of pattern completion in the hippocampal CA3 network. Science.
American Association for the Advancement of Science. https://doi.org/10.1126/science.aaf1836
chicago: Guzmán, José, Alois Schlögl, Michael Frotscher, and Peter M Jonas. “Synaptic
Mechanisms of Pattern Completion in the Hippocampal CA3 Network.” Science.
American Association for the Advancement of Science, 2016. https://doi.org/10.1126/science.aaf1836.
ieee: J. Guzmán, A. Schlögl, M. Frotscher, and P. M. Jonas, “Synaptic mechanisms
of pattern completion in the hippocampal CA3 network,” Science, vol. 353,
no. 6304. American Association for the Advancement of Science, pp. 1117–1123,
2016.
ista: Guzmán J, Schlögl A, Frotscher M, Jonas PM. 2016. Synaptic mechanisms of pattern
completion in the hippocampal CA3 network. Science. 353(6304), 1117–1123.
mla: Guzmán, José, et al. “Synaptic Mechanisms of Pattern Completion in the Hippocampal
CA3 Network.” Science, vol. 353, no. 6304, American Association for the
Advancement of Science, 2016, pp. 1117–23, doi:10.1126/science.aaf1836.
short: J. Guzmán, A. Schlögl, M. Frotscher, P.M. Jonas, Science 353 (2016) 1117–1123.
corr_author: '1'
date_created: 2018-12-11T11:51:31Z
date_published: 2016-09-09T00:00:00Z
date_updated: 2025-09-22T08:12:10Z
day: '09'
ddc:
- '570'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.1126/science.aaf1836
ec_funded: 1
external_id:
isi:
- '000382626800045'
file:
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date_created: 2018-12-12T10:12:27Z
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fulldoi: https://doi.org/10.1126/science.aaf1836
has_accepted_license: '1'
intvolume: ' 353'
isi: 1
issue: '6304'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Preprint
page: 1117 - 1123
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '5899'
pubrep_id: '823'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Synaptic mechanisms of pattern completion in the hippocampal CA3 network
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 353
year: '2016'
...
---
_id: '1435'
abstract:
- lang: eng
text: ATP released from neurons and astrocytes during neuronal activity or under
pathophysiological circumstances is able to influence information flow in neuronal
circuits by activation of ionotropic P2X and metabotropic P2Y receptors and subsequent
modulation of cellular excitability, synaptic strength, and plasticity. In the
present paper we review cellular and network effects of P2Y receptors in the brain.
We show that P2Y receptors inhibit the release of neurotransmitters, modulate
voltage- and ligand-gated ion channels, and differentially influence the induction
of synaptic plasticity in the prefrontal cortex, hippocampus, and cerebellum.
The findings discussed here may explain how P2Y1 receptor activation during brain
injury, hypoxia, inflammation, schizophrenia, or Alzheimer's disease leads to
an impairment of cognitive processes. Hence, it is suggested that the blockade
of P2Y1 receptors may have therapeutic potential against cognitive disturbances
in these states.
article_number: '1207393'
article_processing_charge: No
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
orcid: 0000-0003-2209-5242
- first_name: Zoltan
full_name: Gerevich, Zoltan
last_name: Gerevich
citation:
ama: 'Guzmán J, Gerevich Z. P2Y receptors in synaptic transmission and plasticity:
Therapeutic potential in cognitive dysfunction. Neural Plasticity. 2016;2016.
doi:10.1155/2016/1207393'
apa: 'Guzmán, J., & Gerevich, Z. (2016). P2Y receptors in synaptic transmission
and plasticity: Therapeutic potential in cognitive dysfunction. Neural Plasticity.
Hindawi Publishing Corporation. https://doi.org/10.1155/2016/1207393'
chicago: 'Guzmán, José, and Zoltan Gerevich. “P2Y Receptors in Synaptic Transmission
and Plasticity: Therapeutic Potential in Cognitive Dysfunction.” Neural Plasticity.
Hindawi Publishing Corporation, 2016. https://doi.org/10.1155/2016/1207393.'
ieee: 'J. Guzmán and Z. Gerevich, “P2Y receptors in synaptic transmission and plasticity:
Therapeutic potential in cognitive dysfunction,” Neural Plasticity, vol.
2016. Hindawi Publishing Corporation, 2016.'
ista: 'Guzmán J, Gerevich Z. 2016. P2Y receptors in synaptic transmission and plasticity:
Therapeutic potential in cognitive dysfunction. Neural Plasticity. 2016, 1207393.'
mla: 'Guzmán, José, and Zoltan Gerevich. “P2Y Receptors in Synaptic Transmission
and Plasticity: Therapeutic Potential in Cognitive Dysfunction.” Neural Plasticity,
vol. 2016, 1207393, Hindawi Publishing Corporation, 2016, doi:10.1155/2016/1207393.'
short: J. Guzmán, Z. Gerevich, Neural Plasticity 2016 (2016).
date_created: 2018-12-11T11:52:00Z
date_published: 2016-01-01T00:00:00Z
date_updated: 2025-09-18T11:45:18Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1155/2016/1207393
external_id:
isi:
- '000374056200001'
file:
- access_level: open_access
checksum: 8dc5c2f3d44d4775a6e7e3edb0d7a0da
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:17Z
date_updated: 2020-07-14T12:44:54Z
file_id: '4740'
file_name: IST-2016-580-v1+1_1207393.pdf
file_size: 1395180
relation: main_file
file_date_updated: 2020-07-14T12:44:54Z
fulldoi: https://doi.org/10.1155/2016/1207393
has_accepted_license: '1'
intvolume: ' 2016'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: Neural Plasticity
publication_status: published
publisher: Hindawi Publishing Corporation
publist_id: '5762'
pubrep_id: '580'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'P2Y receptors in synaptic transmission and plasticity: Therapeutic potential
in cognitive dysfunction'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 2016
year: '2016'
...