@article{21767,
  abstract     = {The involvement of non-scientific staff in discussions about animal welfare and scientific quality is essential for biomedical research progress. In this study, we developed a survey to collect the self-perception of animal care staff (ACS) and laboratory technicians about their involvement in scientific planning and conduct. Participants were contacted to complete an anonymous online questionnaire. We obtained 850 responses, mainly from Europe: 564 from ACS and 286 from laboratory technicians. Job satisfaction was assessed as positive by ACS and laboratory technicians despite the low frequency of culture of care activities and mental health meetings. Both groups expressed their desire to be trained in research planning and conduct; however, regular training was not reported. In addition, the inability to act on animal welfare concerns owing to experimental reasons was reported by both groups. Over half of the participants felt valued and appreciated by the lead scientists or animal facility manager; however, it is not clear how they are acknowledged, as their names on the authors list or in the manuscript acknowledgments are barely included. Our results indicated that involvement of ACS and laboratory technicians in planning and conducting studies would improve their understanding of how experiments are done, and therefore communication processes, work satisfaction, animal welfare, and scientific quality. Finally, we provided recommendations to improve the engagement of ACS and laboratory technicians in discussions about animal research planning and conduct.},
  author       = {Gonzalez-Uarquin, Fernando and Jirkof, Paulin and Bert, Bettina and Hawkins, Penny and Angelovski, Ljupco and Baumgart, Jan and Baumgart, Nadine and Cevik, Özge S. and Franco, Nuno H. and Horata, Erdal and Kaura, Rohish and Neuhaus, Winfried and Riso, Brigida and Smith, Adrian J. and Sotiropoulos, Athanassia and Vitale, Augusto and Schober, Sophie},
  issn         = {1758-1117},
  journal      = {Laboratory Animals},
  publisher    = {SAGE Publications},
  title        = {{Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science}},
  doi          = {10.1177/00236772251400976},
  year         = {2026},
}

@article{21848,
  abstract     = {Despite the success of mRNA therapeutics, challenges remain in optimizing immune responses and minimizing side effects. Cell-specific antigen delivery may help reduce required doses and improve vaccine efficacy. In this study, we report on a targeted delivery system for mRNA to a specific subset of skin-resident antigen-presenting cells: Langerhans cells. By functionalizing lipid nanoparticles with a langerin-specific glycomimetic ligand, we achieve selective mRNA delivery to both murine and human primary Langerhans cells with minimal off-target uptake, at the same time resulting in significantly increased mRNA translation. This targeted mRNA delivery not only enhances antigen presentation and T-cell responses but also enables dose-sparing and superior antitumor immunity compared with conventional immunization in a B16-OVA tumor model. Importantly, our platform’s high compatibility with various lipid nanoparticle formulations offers a flexible and precise tool for skin-directed mRNA delivery.},
  author       = {Klein, Klara and Johnson, Litty and Rîca, Ramona and Sarcevic, Mirza and Carta, Gabriele and Seiser, Saskia and Elbe-Bürger, Adelheid and Langer, Freyja and Rahhal, Nowras and Rademacher, Christoph and Wawrzinek, Robert and Quattrone, Federica and Sparber, Florian},
  issn         = {1523-1747},
  journal      = {Journal of Investigative Dermatology},
  publisher    = {Elsevier},
  title        = {{Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity}},
  doi          = {10.1016/j.jid.2026.03.026},
  year         = {2026},
}

@article{21950,
  abstract     = {One Health initiatives are modern paradigms for research and health care practices in various fields. Concrete definitions of the One Health framework, however, remain heterogeneous, leading to conceptual problems and uncertainties in the application of the framework. This article discusses several approaches to the One Health concept, and their associated consequences, with special focus on animal experimentation. The first issue addressed is how One Health should be defined, as well as what (and who) should be considered within a One Health approach. In order to shed further light on this, we explore the history of animals in biomedical science, highlighting historical milestones in the use of animal models, as well as the development and current state of ethical considerations in the field of animal experimentation. The second issue comes with the inclusion of animal experimentation per se as part of the One Health concept. Therefore, particular attention is paid to bioethical principles and the resulting problems that can arise when applying them to the One Health concept. Arguments such as the idea of inequality between humans and non-human animals, and the premise that all actions are done for the benefit of humans, are raised and then used to explore the question of whether the One Health concept is compatible with existing bioethical principles. Based on the bioethical principles of protecting the environment, the biodiversity and biosphere, this paper seeks an inclusive perspective of the One Health concept. Successful solutions will be based on this concept, which embraces all living beings. The authors conclude that a multispecies ethics approach could help create a more ethical ecosystem that is aligned with the wellbeing of all life on a shared planet.},
  author       = {Ulman, Yesim Isil and Kostomitsopoulos, Nikos and Camenzind, Samuel and Kitsara, Maria and Pavone, Ilja Richard and Schober, Sophie},
  issn         = {2632-3559},
  journal      = {Alternatives to Laboratory Animals},
  publisher    = {SAGE Publications},
  title        = {{Emerging bioethical conflicts: One Health and animal experimentation}},
  doi          = {10.1177/02611929261453330},
  year         = {2026},
}

@unpublished{21963,
  abstract     = {The cerebral cortex consists of immense numbers of neuronal and glial cell-types derived from radial glial progenitor (RGP) cells. How RGPs generate appropriate quantities of distinct cortical cell-types to safeguard a brain of correct size, is not well understood. However, genetic aberration in human, including mutations in PTEN, lead to cortical malformation such as macrocephaly, albeit with unknown etiology. Here we utilized Mosaic Analysis with Double Markers (MADM)-based clonal analysis and single cell phenotyping to decipher the role of Pten in neurogenic and gliogenic RGP lineage progression during cortical ontogeny. While neurogenic RGP lineage progression and projection neuron production was moderately altered in the absence of Pten, cortical astrocyte production was drastically increased. Through genetic epistasis experiments we show that the loss of Pten uncouples astrocyte generation from essential growth factor signaling hubs, funneling into MAPK. Collectively, our results suggest that Pten regulates RGP lineage progression with distinct sequential functions in cortical projection neurogenesis and astrocyte production to ensure the emergence of a correctly-sized cerebral cortex.},
  author       = {Miranda, Osvaldo and Contreras, Ximena and Pauler, Florian and Davaatseren, Amarbayasgalan and Amberg, Nicole and Streicher, Carmen and Villalba Requena, Ana and Heger, Anna-Magdalena and Marie, Corentine and Hassan, Bassem A. and Rülicke, Thomas and Hippenmeyer, Simon},
  booktitle    = {bioRxiv},
  title        = {{Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production}},
  doi          = {10.64898/2026.05.01.722191},
  year         = {2026},
}

@article{20858,
  abstract     = {Targeted antigen delivery to immune cells, particularly dendritic cells, has emerged as a promising strategy to enhance therapeutic efficacy of vaccines, while minimizing adverse effects associated with conventional immunization. In this study, we use our previously described small glycomimetic molecule that is selectively recognized by the Langerhans cell (LC)-specific surface receptor Langerin and demonstrate specific delivery of protein antigens to these specialized dendritic cells. Our results show that Langerin-mediated antigen delivery significantly enhances the immune response in vivo, resulting in increased expansion and activation of antigen-specific T cells, compared to immunization with unmodified antigen. We demonstrate the feasibility of our LC-targeted platform for immune cell-specific immunization with protein antigen and underscore the potential of LCs as an access point for next-generation vaccines and immunotherapies.},
  author       = {Rica, Ramona and Klein, Klara and Johnson, Litty and Carta, Gabriele and Sarcevic, Mirza and Langer, Freyja and Rademacher, Christoph and Wawrzinek, Robert and Quattrone, Federica and Sparber, Florian},
  issn         = {1525-0024},
  journal      = {Molecular Therapy},
  publisher    = {Elsevier},
  title        = {{Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response}},
  doi          = {10.1016/j.ymthe.2025.10.008},
  year         = {2025},
}

@article{18449,
  abstract     = {Research involving human subjects or identifiable human material and data must be assessed by an ethics committee. The Karl Landsteiner University of Health Sciences has established a Commission on Ethics and Scientific Integrity to evaluate medical research conducted by its faculty and students and at its affiliated hospitals.
All projects submitted to the Commission on Ethics and Scientific Integrity between 2018 and 2023 were analyzed regarding their major characteristics, the duration of the evaluation process, and votes issued.
A total of 520 applications were electronically submitted during the observation period. Most of the studies were retrospective data analyses in the field of oncology, psychology and surgery. Most studies included less than 100 volunteers. Of the applications 50% received a final vote within 5 months, during which several revision rounds took place. Overall, about 77% of votes issued during the observation period were positive and 2% were rejections. In 11% files were closed due to withdrawal. In 11% final votes were pending at the end of the observation period due to requests for revisions.
Our results emphasize the importance of institutional ethics committees using the example of the Commission on Ethics and Scientific Integrity at the Karl Landsteiner University. Such committees fill a gap in evaluating research not covered by Austrian legal regulations. Continuous development of standards, operating procedures, and national and international collaborations are required to assess and minimize risks to trial subjects and to provide a safe and productive environment for research in human medicine and related fields.},
  author       = {Schober, Sophie and Klee, Sascha and Trautinger, Franz},
  issn         = {1613-7671},
  journal      = {Wiener Klinische Wochenschrift},
  pages        = {432--437},
  publisher    = {Springer Nature},
  title        = {{The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023}},
  doi          = {10.1007/s00508-024-02462-x},
  volume       = {137},
  year         = {2025},
}

@article{19076,
  abstract     = {For accurate perception and motor control, an animal must distinguish between sensory experiences elicited by external stimuli and those elicited by its own actions. The diversity of behaviors and their complex influences on the senses make this distinction challenging. Here, we uncover an action–cue hub that coordinates motor commands with visual processing in the brain’s first visual relay. We show that the ventral lateral geniculate nucleus (vLGN) acts as a corollary discharge center, integrating visual translational optic flow signals with motor copies from saccades, locomotion and pupil dynamics. The vLGN relays these signals to correct action-specific visual distortions and to refine perception, as shown for the superior colliculus and in a depth-estimation task. Simultaneously, brain-wide vLGN projections drive corrective actions necessary for accurate visuomotor control. Our results reveal an extended corollary discharge architecture that refines early visual transformations and coordinates actions via a distributed hub-and-spoke network to enable visual perception during action.},
  author       = {Vega Zuniga, Tomas A and Sumser, Anton L and Symonova, Olga and Koppensteiner, Peter and Schmidt, Florian and Jösch, Maximilian A},
  issn         = {1546-1726},
  journal      = {Nature Neuroscience},
  publisher    = {Springer Nature},
  title        = {{A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics}},
  doi          = {10.1038/s41593-025-01874-w},
  volume       = {28},
  year         = {2025},
}

@article{15164,
  abstract     = {Primary implant stability, which refers to the stability of the implant during the initial healing period is a crucial factor in determining the long-term success of the implant and lays the foundation for secondary implant stability achieved through osseointegration. Factors affecting primary stability include implant design, surgical technique, and patient-specific factors like bone quality and morphology. In vivo, the cyclic nature of anatomical loading puts osteosynthesis locking screws under dynamic loads, which can lead to the formation of micro cracks and defects that slowly degrade the mechanical connection between the bone and screw, thus compromising the initial stability and secondary stability of the implant. Monotonic quasi-static loading used for testing the holding capacity of implanted screws is not well suited to capture this behavior since it cannot capture the progressive deterioration of peri‑implant bone at small displacements. In order to address this issue, this study aims to determine a critical point of loss of primary implant stability in osteosynthesis locking screws under cyclic overloading by investigating the evolution of damage, dissipated energy, and permanent deformation. A custom-made test setup was used to test implanted 2.5 mm locking screws under cyclic overloading test. For each loading cycle, maximum forces and displacement were recorded as well as initial and final cycle displacements and used to calculate damage and energy dissipation evolution. The results of this study demonstrate that for axial, shear, and mixed loading significant damage and energy dissipation can be observed at approximately 20 % of the failure force. Additionally, at this load level, permanent deformations on the screw-bone interface were found to be in the range of 50 to 150 mm which promotes osseointegration and secondary implant stability. This research can assist surgeons in making informed preoperative decisions by providing a better understanding of the critical point of loss of primary implant stability, thus improving the long-term success of the implant and overall patient satisfaction.},
  author       = {Silva-Henao, Juan D. and Schober, Sophie and Pahr, Dieter H. and Reisinger, Andreas G.},
  issn         = {1873-4030},
  journal      = {Medical Engineering and Physics},
  publisher    = {Elsevier},
  title        = {{Critical loss of primary implant stability in osteosynthesis locking screws under cyclic overloading}},
  doi          = {10.1016/j.medengphy.2024.104143},
  volume       = {126},
  year         = {2024},
}

@misc{15385,
  abstract     = {Relevant information about the data can be found in the 'Readme_Data.txt' file. 
A previous version of the publication can be found on BioRxiv: https://www.biorxiv.org/content/10.1101/2022.10.11.511691v4
and published in Plos Biology (2024)},
  author       = {Burnett, Laura and Koppensteiner, Peter and Symonova, Olga and Masson, Tomas and Vega Zuniga, Tomas A and Contreras, Ximena and Rülicke, Thomas and Shigemoto, Ryuichi and Novarino, Gaia and Jösch, Maximilian A},
  keywords     = {ASD, periaqueductal gray, perception, behavior, potassium channels},
  publisher    = {Institute of Science and Technology Austria},
  title        = {{Shared behavioural impairments in visual perception and place avoidance across different autism models are driven by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice}},
  doi          = {10.15479/AT:ISTA:15385},
  year         = {2024},
}

@article{18587,
  abstract     = {Many scientific breakthroughs have depended on animal research, yet the ethical concerns surrounding the use of animals in experimentation have long prompted discussions about humane treatment and responsible scientific practice. First articulated by Russell and Burch, the 3Rs Principles of Replacement, Reduction, and Refinement have gained widespread recognition as basic guidelines for animal research. Over time, the 3Rs have transcended the research community, influencing policy decisions, animal welfare advocacy and public perception of animal experimentation. Despite their broad acceptance, interpretations of the 3Rs vary substantially, shaping statutory frameworks at various levels, with both technical and practical impacts.},
  author       = {Lauwereyns, Jan and Bajramovic, Jeffrey and Bert, Bettina and Camenzind, Samuel and De Kock, Joery and Elezović, Alisa and Erden, Sevilay and Gonzalez-Uarquin, Fernando and Ulman, Yesim Isil and Hoffmann, Orsolya Ivett and Kitsara, Maria and Kostomitsopoulos, Nikolaos and Neuhaus, Winfried and Petit-Demouliere, Benoit and Pollo, Simone and Riso, Brígida and Schober, Sophie and Sotiropoulos, Athanassia and Thomas, Aurélie and Vitale, Augusto and Wilflingseder, Doris and Ahluwalia, Arti},
  issn         = {1548-4475},
  journal      = {Lab Animal},
  pages        = {347--350},
  publisher    = {Springer Nature},
  title        = {{Toward a common interpretation of the 3Rs principles in animal research}},
  doi          = {10.1038/s41684-024-01476-2},
  volume       = {53},
  year         = {2024},
}

@article{17232,
  abstract     = {The lineage relationship of clonally-related cells offers important insights into the ontogeny and cytoarchitecture of the brain in health and disease. Here, we provide a protocol to concurrently assess cell lineage relationship and cell-type identity among clonally-related cells in situ. We first describe the preparation and screening of acute brain slices containing clonally-related cells labeled using mosaic analysis with double markers (MADM). We then outline steps to collect RNA from individual cells for downstream applications and cell-type identification using RNA sequencing.
For complete details on the use and execution of this protocol, please refer to Cheung et al.
1},
  author       = {Cheung, Giselle T and Pauler, Florian and Koppensteiner, Peter and Hippenmeyer, Simon},
  issn         = {2666-1667},
  journal      = {STAR Protocols},
  number       = {3},
  publisher    = {Elsevier},
  title        = {{Protocol for mapping cell lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq}},
  doi          = {10.1016/j.xpro.2024.103168},
  volume       = {5},
  year         = {2024},
}

@article{17280,
  abstract     = {Adherens junction–associated protein 1 (AJAP1) has been implicated in brain diseases; however, a pathogenic mechanism has not been identified. AJAP1 is widely expressed in neurons and binds to γ-aminobutyric acid type B receptors (GBRs), which inhibit neurotransmitter release at most synapses in the brain. Here, we show that AJAP1 is selectively expressed in dendrites and trans-synaptically recruits GBRs to presynaptic sites of neurons expressing AJAP1. We have identified several monoallelic AJAP1 variants in individuals with epilepsy and/or neurodevelopmental disorders. Specifically, we show that the variant p.(W183C) lacks binding to GBRs, resulting in the inability to recruit them. Ultrastructural analysis revealed significantly decreased presynaptic GBR levels in Ajap1−/− and Ajap1W183C/+ mice. Consequently, these mice exhibited reduced GBR-mediated presynaptic inhibition at excitatory and inhibitory synapses, along with impaired synaptic plasticity. Our study reveals that AJAP1 enables the postsynaptic neuron to regulate the level of presynaptic GBR-mediated inhibition, supporting the clinical relevance of loss-of-function AJAP1 variants.},
  author       = {Früh, Simon and Boudkkazi, Sami and Koppensteiner, Peter and Sereikaite, Vita and Chen, Li Yuan and Fernandez-Fernandez, Diego and Rem, Pascal D. and Ulrich, Daniel and Schwenk, Jochen and Chen, Ziyang and Monnier, Elodie Le and Fritzius, Thorsten and Innocenti, Sabrina M. and Besseyrias, Valérie and Trovò, Luca and Stawarski, Michal and Argilli, Emanuela and Sherr, Elliott H. and Van Bon, Bregje and Kamsteeg, Erik Jan and Iascone, Maria and Pilotta, Alba and Cutrì, Maria R. and Azamian, Mahshid S. and Hernández-García, Andrés and Lalani, Seema R. and Rosenfeld, Jill A. and Zhao, Xiaonan and Vogel, Tiphanie P. and Ona, Herda and Scott, Daryl A. and Scheiffele, Peter and Strømgaard, Kristian and Tafti, Mehdi and Gassmann, Martin and Fakler, Bernd and Shigemoto, Ryuichi and Bettler, Bernhard},
  issn         = {2375-2548},
  journal      = {Science Advances},
  number       = {28},
  publisher    = {American Association for the Advancement of Science},
  title        = {{Monoallelic de novo AJAP1 loss-of- function variants disrupt trans-synaptic control of neurotransmitter release}},
  doi          = {10.1126/sciadv.adk5462},
  volume       = {10},
  year         = {2024},
}

@article{18310,
  author       = {Kitsara, Maria and Smajlhodžić-Deljo, Merima and Gurbeta Pokvic, Lejla and Bert, Bettina and Bubalo, Nataliia and Erden, Sevilay and Franco, Nuno Henrique and Chirico, Giuseppe and Gómez Raja, Jonathan and Gonzalez-Uarquin, Fernando and Lang, Annemarie and Linklater, Nicole and Mojsova, Sandra and Olsson, I. Anna S. and Sandvig, Ioanna and Schaffert, Alexandra and Schmit, Marthe and Schober, Sophie and Sevastre, Bogdan and Wilflingseder, Doris and Ahluwalia, Arti and Neuhaus, Winfried},
  issn         = {2632-3559},
  journal      = {Alternatives to Laboratory Animals},
  number       = {6},
  pages        = {326--333},
  publisher    = {SAGE Publications},
  title        = {{Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE)}},
  doi          = {10.1177/02611929241286024},
  volume       = {52},
  year         = {2024},
}

@article{12224,
  abstract     = {Muskelin (Mkln1) is implicated in neuronal function, regulating plasma membrane receptor trafficking. However, its influence on intrinsic brain activity and corresponding behavioral processes remains unclear. Here we show that murine <jats:italic>Mkln1</jats:italic> knockout causes non-habituating locomotor activity, increased exploratory drive, and decreased locomotor response to amphetamine. Muskelin deficiency impairs social novelty detection while promoting the retention of spatial reference memory and fear extinction recall. This is strongly mirrored in either weaker or stronger resting-state functional connectivity between critical circuits mediating locomotor exploration and cognition. We show that <jats:italic>Mkln1</jats:italic> deletion alters dendrite branching and spine structure, coinciding with enhanced AMPAR-mediated synaptic transmission but selective impairment in synaptic potentiation maintenance. We identify muskelin at excitatory synapses and highlight its role in regulating dendritic spine actin stability. Our findings point to aberrant spine actin modulation and changes in glutamatergic synaptic function as critical mechanisms that contribute to the neurobehavioral phenotype arising from <jats:italic>Mkln1</jats:italic> ablation.},
  author       = {Muhia, Mary W and YuanXiang, PingAn and Sedlacik, Jan and Schwarz, Jürgen R. and Heisler, Frank F. and Gromova, Kira V. and Thies, Edda and Breiden, Petra and Pechmann, Yvonne and Kreutz, Michael R. and Kneussel, Matthias},
  issn         = {2399-3642},
  journal      = {Communications Biology},
  keywords     = {General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, Medicine (miscellaneous)},
  publisher    = {Springer Nature},
  title        = {{Muskelin regulates actin-dependent synaptic changes and intrinsic brain activity relevant to behavioral and cognitive processes}},
  doi          = {10.1038/s42003-022-03446-1},
  volume       = {5},
  year         = {2022},
}

@article{10283,
  abstract     = {During the past decade, the scientific community and outside observers have noted a concerning lack of rigor and transparency in preclinical research that led to talk of a “reproducibility crisis” in the life sciences (Baker, 2016; Bespalov & Steckler, 2018; Heddleston et al, 2021). Various measures have been proposed to address the problem: from better training of scientists to more oversight to expanded publishing practices such as preregistration of studies. The recently published EQIPD (Enhancing Quality in Preclinical Data) System is, to date, the largest initiative that aims to establish a systematic approach for increasing the robustness and reliability of biomedical research (Bespalov et al, 2021). However, promoting a cultural change in research practices warrants a broad adoption of the Quality System and its underlying philosophy. It is here that academic Core Facilities (CF), research service providers at universities and research institutions, can make a difference. It is fair to assume that a significant fraction of published data originated from experiments that were designed, run, or analyzed in CFs. These academic services play an important role in the research ecosystem by offering access to cutting-edge equipment and by developing and testing novel techniques and methods that impact research in the academic and private sectors alike (Bikovski et al, 2020). Equipment and infrastructure are not the only value: CFs employ competent personnel with profound knowledge and practical experience of the specific field of interest: animal behavior, imaging, crystallography, genomics, and so on. Thus, CFs are optimally positioned to address concerns about the quality and robustness of preclinical research.},
  author       = {Restivo, Leonardo and Gerlach, Björn and Tsoory, Michael and Bikovski, Lior and Badurek, Sylvia and Pitzer, Claudia and Kos-Braun, Isabelle C. and Mausset-Bonnefont, Anne Laure Mj and Ward, Jonathan and Schunn, Michael and Noldus, Lucas P.J.J. and Bespalov, Anton and Voikar, Vootele},
  issn         = {1469-3178},
  journal      = {EMBO Reports},
  publisher    = {EMBO Press},
  title        = {{Towards best practices in research: Role of academic core facilities}},
  doi          = {10.15252/embr.202153824},
  volume       = {22},
  year         = {2021},
}

@article{9603,
  abstract     = {Mosaic analysis with double markers (MADM) offers one approach to visualize and concomitantly manipulate genetically defined cells in mice with single-cell resolution. MADM applications include the analysis of lineage, single-cell morphology and physiology, genomic imprinting phenotypes, and dissection of cell-autonomous gene functions in vivo in health and disease. Yet, MADM can only be applied to <25% of all mouse genes on select chromosomes to date. To overcome this limitation, we generate transgenic mice with knocked-in MADM cassettes near the centromeres of all 19 autosomes and validate their use across organs. With this resource, >96% of the entire mouse genome can now be subjected to single-cell genetic mosaic analysis. Beyond a proof of principle, we apply our MADM library to systematically trace sister chromatid segregation in distinct mitotic cell lineages. We find striking chromosome-specific biases in segregation patterns, reflecting a putative mechanism for the asymmetric segregation of genetic determinants in somatic stem cell division.},
  author       = {Contreras, Ximena and Amberg, Nicole and Davaatseren, Amarbayasgalan and Hansen, Andi H and Sonntag, Johanna and Andersen, Lill and Bernthaler, Tina and Streicher, Carmen and Heger, Anna-Magdalena and Johnson, Randy L. and Schwarz, Lindsay A. and Luo, Liqun and Rülicke, Thomas and Hippenmeyer, Simon},
  issn         = {2211-1247},
  journal      = {Cell Reports},
  number       = {12},
  publisher    = {Cell Press},
  title        = {{A genome-wide library of MADM mice for single-cell genetic mosaic analysis}},
  doi          = {10.1016/j.celrep.2021.109274},
  volume       = {35},
  year         = {2021},
}

@article{9607,
  abstract     = {While high risk of failure is an inherent part of developing innovative therapies, it can be reduced by adherence to evidence-based rigorous research practices. Numerous analyses conducted to date have clearly identified measures that need to be taken to improve research rigor. Supported through the European Union's Innovative Medicines Initiative, the EQIPD consortium has developed a novel preclinical research quality system that can be applied in both public and private sectors and is free for anyone to use. The EQIPD Quality System was designed to be suited to boost innovation by ensuring the generation of robust and reliable preclinical data while being lean, effective and not becoming a burden that could negatively impact the freedom to explore scientific questions. EQIPD defines research quality as the extent to which research data are fit for their intended use. Fitness, in this context, is defined by the stakeholders, who are the scientists directly involved in the research, but also their funders, sponsors, publishers, research tool manufacturers and collaboration partners such as peers in a multi-site research project. The essence of the EQIPD Quality System is the set of 18 core requirements that can be addressed flexibly, according to user-specific needs and following a user-defined trajectory. The EQIPD Quality System proposes guidance on expectations for quality-related measures, defines criteria for adequate processes (i.e., performance standards) and provides examples of how such measures can be developed and implemented. However, it does not prescribe any pre-determined solutions. EQIPD has also developed tools (for optional use) to support users in implementing the system and assessment services for those research units that successfully implement the quality system and seek formal accreditation. Building upon the feedback from users and continuous improvement, a sustainable EQIPD Quality System will ultimately serve the entire community of scientists conducting non-regulated preclinical research, by helping them generate reliable data that are fit for their intended use.},
  author       = {Bespalov, Anton and Bernard, René and Gilis, Anja and Gerlach, Björn and Guillén, Javier and Castagné, Vincent and Lefevre, Isabel A. and Ducrey, Fiona and Monk, Lee and Bongiovanni, Sandrine and Altevogt, Bruce and Arroyo-Araujo, María and Bikovski, Lior and De Bruin, Natasja and Castaños-Vélez, Esmeralda and Dityatev, Alexander and Emmerich, Christoph H. and Fares, Raafat and Ferland-Beckham, Chantelle and Froger-Colléaux, Christelle and Gailus-Durner, Valerie and Hölter, Sabine M. and Hofmann, Martine Cj and Kabitzke, Patricia and Kas, Martien Jh and Kurreck, Claudia and Moser, Paul and Pietraszek, Malgorzata and Popik, Piotr and Potschka, Heidrun and Prado Montes De Oca, Ernesto and Restivo, Leonardo and Riedel, Gernot and Ritskes-Hoitinga, Merel and Samardzic, Janko and Schunn, Michael and Stöger, Claudia and Voikar, Vootele and Vollert, Jan and Wever, Kimberley E. and Wuyts, Kathleen and Macleod, Malcolm R. and Dirnagl, Ulrich and Steckler, Thomas},
  issn         = {2050-084X},
  journal      = {eLife},
  publisher    = {eLife Sciences Publications},
  title        = {{Introduction to the EQIPD quality system}},
  doi          = {10.7554/eLife.63294},
  volume       = {10},
  year         = {2021},
}

@article{6607,
  abstract     = {Acute myeloid leukemia (AML) is a heterogeneous disease with respect to its genetic and molecular basis and to patients´ outcome. Clinical, cytogenetic, and mutational data are used to classify patients into risk groups with different survival, however, within-group heterogeneity is still an issue. Here, we used a robust likelihood-based survival modeling approach and publicly available gene expression data to identify a minimal number of genes whose combined expression values were prognostic of overall survival. The resulting gene expression signature (4-GES) consisted of 4 genes (SOCS2, IL2RA, NPDC1, PHGDH), predicted patient survival as an independent prognostic parameter in several cohorts of AML patients (total, 1272 patients), and further refined prognostication based on the European Leukemia Net classification. An oncogenic role of the top scoring gene in this signature, SOCS2, was investigated using MLL-AF9 and Flt3-ITD/NPM1c driven mouse models of AML. SOCS2 promoted leukemogenesis as well as the abundance, quiescence, and activity of AML stem cells. Overall, the 4-GES represents a highly discriminating prognostic parameter in AML, whose clinical applicability is greatly enhanced by its small number of genes. The newly established role of SOCS2 in leukemia aggressiveness and stemness raises the possibility that the signature might even be exploitable therapeutically.},
  author       = {Nguyen, Chi Huu and Glüxam, Tobias and Schlerka, Angela and Bauer, Katharina and Grandits, Alexander M. and Hackl, Hubert and Dovey, Oliver and Zöchbauer-Müller, Sabine and Cooper, Jonathan L. and Vassiliou, George S. and Stoiber, Dagmar and Wieser, Rotraud and Heller, Gerwin},
  journal      = {Scientific Reports},
  number       = {1},
  publisher    = {Nature Publishing Group},
  title        = {{SOCS2 is part of a highly prognostic 4-gene signature in AML and promotes disease aggressiveness}},
  doi          = {10.1038/s41598-019-45579-0},
  volume       = {9},
  year         = {2019},
}