[{"status":"public","day":"01","ec_funded":1,"citation":{"apa":"Makohon Moore, A., Zhang, M., Reiter, J., Božić, I., Allen, B., Kundu, D., … Iacobuzio Donahue, C. (2017). Limited heterogeneity of known driver gene mutations among the metastases of individual patients with pancreatic cancer. Nature Genetics. Nature Publishing Group. https://doi.org/10.1038/ng.3764","chicago":"Makohon Moore, Alvin, Ming Zhang, Johannes Reiter, Ivana Božić, Benjamin Allen, Deepanjan Kundu, Krishnendu Chatterjee, et al. “Limited Heterogeneity of Known Driver Gene Mutations among the Metastases of Individual Patients with Pancreatic Cancer.” Nature Genetics. Nature Publishing Group, 2017. https://doi.org/10.1038/ng.3764.","mla":"Makohon Moore, Alvin, et al. “Limited Heterogeneity of Known Driver Gene Mutations among the Metastases of Individual Patients with Pancreatic Cancer.” Nature Genetics, vol. 49, no. 3, Nature Publishing Group, 2017, pp. 358–66, doi:10.1038/ng.3764.","short":"A. Makohon Moore, M. Zhang, J. Reiter, I. Božić, B. Allen, D. Kundu, K. Chatterjee, F. Wong, Y. Jiao, Z. Kohutek, J. Hong, M. Attiyeh, B. Javier, L. Wood, R. Hruban, M. Nowak, N. Papadopoulos, K. Kinzler, B. Vogelstein, C. Iacobuzio Donahue, Nature Genetics 49 (2017) 358–366.","ieee":"A. Makohon Moore et al., “Limited heterogeneity of known driver gene mutations among the metastases of individual patients with pancreatic cancer,” Nature Genetics, vol. 49, no. 3. Nature Publishing Group, pp. 358–366, 2017.","ista":"Makohon Moore A, Zhang M, Reiter J, Božić I, Allen B, Kundu D, Chatterjee K, Wong F, Jiao Y, Kohutek Z, Hong J, Attiyeh M, Javier B, Wood L, Hruban R, Nowak M, Papadopoulos N, Kinzler K, Vogelstein B, Iacobuzio Donahue C. 2017. Limited heterogeneity of known driver gene mutations among the metastases of individual patients with pancreatic cancer. Nature Genetics. 49(3), 358–366.","ama":"Makohon Moore A, Zhang M, Reiter J, et al. Limited heterogeneity of known driver gene mutations among the metastases of individual patients with pancreatic cancer. Nature Genetics. 2017;49(3):358-366. doi:10.1038/ng.3764"},"project":[{"call_identifier":"FP7","name":"Quantitative Graph Games: Theory and Applications","_id":"2581B60A-B435-11E9-9278-68D0E5697425","grant_number":"279307"},{"call_identifier":"FWF","name":"Modern Graph Algorithmic Techniques in Formal Verification","_id":"2584A770-B435-11E9-9278-68D0E5697425","grant_number":"P 23499-N23"},{"_id":"25863FF4-B435-11E9-9278-68D0E5697425","grant_number":"S11407","call_identifier":"FWF","name":"Game Theory"}],"publist_id":"7092","_id":"653","scopus_import":"1","article_type":"original","oa_version":"Submitted Version","intvolume":" 49","type":"journal_article","acknowledgement":"We thank the Memorial Sloan Kettering Cancer Center Molecular Cytology core facility for immunohistochemistry staining. This work was supported by Office of Naval Research grant N00014-16-1-2914, the Bill and Melinda Gates Foundation (OPP1148627), and a gift from B. Wu and E. Larson (M.A.N.), National Institutes of Health grants CA179991 (C.A.I.-D. and I.B.), F31 CA180682 (A.P.M.-M.), CA43460 (B.V.), and P50 CA62924, the Monastra Foundation, the Virginia and D.K. Ludwig Fund for Cancer Research, the Lustgarten Foundation for Pancreatic Cancer Research, the Sol Goldman Center for Pancreatic Cancer Research, the Sol Goldman Sequencing Center, ERC Start grant 279307: Graph Games (J.G.R., D.K., and C.K.), Austrian Science Fund (FWF) grant P23499-N23 (J.G.R., D.K., and C.K.), and FWF NFN grant S11407-N23 RiSE/SHiNE (J.G.R., D.K., and C.K.).","month":"03","language":[{"iso":"eng"}],"date_published":"2017-03-01T00:00:00Z","department":[{"_id":"KrCh"}],"publisher":"Nature Publishing Group","date_updated":"2025-09-11T07:12:56Z","isi":1,"oa":1,"file_date_updated":"2020-07-14T12:47:33Z","issue":"3","doi":"10.1038/ng.3764","publication_identifier":{"issn":["1061-4036"]},"publication":"Nature Genetics","year":"2017","date_created":"2018-12-11T11:47:43Z","has_accepted_license":"1","ddc":["000"],"quality_controlled":"1","file":[{"date_created":"2019-11-19T08:13:50Z","file_name":"2017_NatureGenetics_Makohon.pdf","content_type":"application/pdf","creator":"dernst","file_id":"7050","relation":"main_file","checksum":"e442dc3b7420a36ec805e9bb45cc1a2e","access_level":"open_access","date_updated":"2020-07-14T12:47:33Z","file_size":908099}],"pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"full_name":"Makohon Moore, Alvin","first_name":"Alvin","last_name":"Makohon Moore"},{"full_name":"Zhang, Ming","last_name":"Zhang","first_name":"Ming"},{"full_name":"Reiter, Johannes","id":"4A918E98-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0170-7353","last_name":"Reiter","first_name":"Johannes"},{"full_name":"Božić, Ivana","last_name":"Božić","first_name":"Ivana"},{"full_name":"Allen, Benjamin","last_name":"Allen","first_name":"Benjamin"},{"full_name":"Kundu, Deepanjan","id":"1d4c0f4f-e8a3-11ec-a351-e36772758c45","first_name":"Deepanjan","last_name":"Kundu"},{"id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4561-241X","full_name":"Chatterjee, Krishnendu","first_name":"Krishnendu","last_name":"Chatterjee"},{"full_name":"Wong, Fay","last_name":"Wong","first_name":"Fay"},{"last_name":"Jiao","first_name":"Yuchen","full_name":"Jiao, Yuchen"},{"full_name":"Kohutek, Zachary","last_name":"Kohutek","first_name":"Zachary"},{"full_name":"Hong, Jungeui","first_name":"Jungeui","last_name":"Hong"},{"full_name":"Attiyeh, Marc","first_name":"Marc","last_name":"Attiyeh"},{"full_name":"Javier, Breanna","last_name":"Javier","first_name":"Breanna"},{"full_name":"Wood, Laura","last_name":"Wood","first_name":"Laura"},{"first_name":"Ralph","last_name":"Hruban","full_name":"Hruban, Ralph"},{"first_name":"Martin","last_name":"Nowak","full_name":"Nowak, Martin"},{"full_name":"Papadopoulos, Nickolas","first_name":"Nickolas","last_name":"Papadopoulos"},{"full_name":"Kinzler, Kenneth","first_name":"Kenneth","last_name":"Kinzler"},{"last_name":"Vogelstein","first_name":"Bert","full_name":"Vogelstein, Bert"},{"last_name":"Iacobuzio Donahue","first_name":"Christine","full_name":"Iacobuzio Donahue, Christine"}],"volume":49,"title":"Limited heterogeneity of known driver gene mutations among the metastases of individual patients with pancreatic cancer","page":"358 - 366","article_processing_charge":"No","abstract":[{"lang":"eng","text":"The extent of heterogeneity among driver gene mutations present in naturally occurring metastases - that is, treatment-naive metastatic disease - is largely unknown. To address this issue, we carried out 60× whole-genome sequencing of 26 metastases from four patients with pancreatic cancer. We found that identical mutations in known driver genes were present in every metastatic lesion for each patient studied. Passenger gene mutations, which do not have known or predicted functional consequences, accounted for all intratumoral heterogeneity. Even with respect to these passenger mutations, our analysis suggests that the genetic similarity among the founding cells of metastases was higher than that expected for any two cells randomly taken from a normal tissue. The uniformity of known driver gene mutations among metastases in the same patient has critical and encouraging implications for the success of future targeted therapies in advanced-stage disease."}],"external_id":{"isi":["000394917800009"],"pmid":["28092682"]},"publication_status":"published"},{"corr_author":"1","file":[{"content_type":"application/pdf","file_name":"IST-2018-987-v1+1_2017_KichevaRivron__Creating_to.pdf","date_created":"2018-12-12T10:15:20Z","creator":"system","file_id":"5139","file_size":228206,"date_updated":"2020-07-14T12:47:33Z","relation":"main_file","checksum":"eef22a0f42a55b232cb2d1188a2322cb","access_level":"open_access"}],"quality_controlled":"1","has_accepted_license":"1","ddc":["571"],"year":"2017","date_created":"2018-12-11T11:47:44Z","issue":"5","publication_identifier":{"issn":["0950-1991"]},"doi":"10.1242/dev.144915","publication":"Development","page":"733 - 736","article_processing_charge":"No","abstract":[{"lang":"eng","text":"In November 2016, developmental biologists, synthetic biologists and engineers gathered in Paris for a meeting called ‘Engineering the embryo’. The participants shared an interest in exploring how synthetic systems can reveal new principles of embryonic development, and how the in vitro manipulation and modeling of development using stem cells can be used to integrate ideas and expertise from physics, developmental biology and tissue engineering. As we review here, the conference pinpointed some of the challenges arising at the intersection of these fields, along with great enthusiasm for finding new approaches and collaborations."}],"external_id":{"isi":["000395650100001"]},"publication_status":"published","title":"Creating to understand – developmental biology meets engineering in Paris","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","author":[{"full_name":"Kicheva, Anna","orcid":"0000-0003-4509-4998","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","last_name":"Kicheva","first_name":"Anna"},{"full_name":"Rivron, Nicolas","first_name":"Nicolas","last_name":"Rivron"}],"volume":144,"citation":{"apa":"Kicheva, A., & Rivron, N. (2017). Creating to understand – developmental biology meets engineering in Paris. Development. Company of Biologists. https://doi.org/10.1242/dev.144915","chicago":"Kicheva, Anna, and Nicolas Rivron. “Creating to Understand – Developmental Biology Meets Engineering in Paris.” Development. Company of Biologists, 2017. https://doi.org/10.1242/dev.144915.","mla":"Kicheva, Anna, and Nicolas Rivron. “Creating to Understand – Developmental Biology Meets Engineering in Paris.” Development, vol. 144, no. 5, Company of Biologists, 2017, pp. 733–36, doi:10.1242/dev.144915.","short":"A. Kicheva, N. Rivron, Development 144 (2017) 733–736.","ieee":"A. Kicheva and N. Rivron, “Creating to understand – developmental biology meets engineering in Paris,” Development, vol. 144, no. 5. Company of Biologists, pp. 733–736, 2017.","ama":"Kicheva A, Rivron N. Creating to understand – developmental biology meets engineering in Paris. Development. 2017;144(5):733-736. doi:10.1242/dev.144915","ista":"Kicheva A, Rivron N. 2017. Creating to understand – developmental biology meets engineering in Paris. Development. 144(5), 733–736."},"project":[{"call_identifier":"H2020","name":"Coordination of Patterning And Growth In the Spinal Cord","_id":"B6FC0238-B512-11E9-945C-1524E6697425","grant_number":"680037"}],"ec_funded":1,"pubrep_id":"987","status":"public","day":"01","isi":1,"oa":1,"file_date_updated":"2020-07-14T12:47:33Z","month":"03","language":[{"iso":"eng"}],"date_published":"2017-03-01T00:00:00Z","publisher":"Company of Biologists","department":[{"_id":"AnKi"}],"date_updated":"2026-04-16T09:59:52Z","intvolume":" 144","oa_version":"Submitted Version","type":"journal_article","publist_id":"7089","_id":"654","scopus_import":"1"},{"volume":6,"author":[{"last_name":"Renault","first_name":"Thibaud","full_name":"Renault, Thibaud"},{"first_name":"Anthony","last_name":"Abraham","full_name":"Abraham, Anthony"},{"id":"2C471CFA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5396-4346","full_name":"Bergmiller, Tobias","last_name":"Bergmiller","first_name":"Tobias"},{"first_name":"Guillaume","last_name":"Paradis","full_name":"Paradis, Guillaume"},{"full_name":"Rainville, Simon","first_name":"Simon","last_name":"Rainville"},{"last_name":"Charpentier","first_name":"Emmanuelle","full_name":"Charpentier, Emmanuelle"},{"id":"47F8433E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6220-2052","full_name":"Guet, Calin C","last_name":"Guet","first_name":"Calin C"},{"last_name":"Tu","first_name":"Yuhai","full_name":"Tu, Yuhai"},{"last_name":"Namba","first_name":"Keiichi","full_name":"Namba, Keiichi"},{"full_name":"Keener, James","last_name":"Keener","first_name":"James"},{"last_name":"Minamino","first_name":"Tohru","full_name":"Minamino, Tohru"},{"full_name":"Erhardt, Marc","last_name":"Erhardt","first_name":"Marc"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"text":"The bacterial flagellum is a self-assembling nanomachine. The external flagellar filament, several times longer than a bacterial cell body, is made of a few tens of thousands subunits of a single protein: flagellin. A fundamental problem concerns the molecular mechanism of how the flagellum grows outside the cell, where no discernible energy source is available. Here, we monitored the dynamic assembly of individual flagella using in situ labelling and real-time immunostaining of elongating flagellar filaments. We report that the rate of flagellum growth, initially ~1,700 amino acids per second, decreases with length and that the previously proposed chain mechanism does not contribute to the filament elongation dynamics. Inhibition of the proton motive force-dependent export apparatus revealed a major contribution of substrate injection in driving filament elongation. The combination of experimental and mathematical evidence demonstrates that a simple, injection-diffusion mechanism controls bacterial flagella growth outside the cell.","lang":"eng"}],"external_id":{"isi":["000398680700001"]},"publication_status":"published","article_processing_charge":"No","title":"Bacterial flagella grow through an injection diffusion mechanism","article_number":"e23136","year":"2017","date_created":"2018-12-11T11:47:44Z","publication":"eLife","publication_identifier":{"issn":["2050-084X"]},"doi":"10.7554/eLife.23136","quality_controlled":"1","file":[{"checksum":"39e1c3e82ddac83a30422fa72fa1a383","relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:47:33Z","file_size":5520359,"file_id":"4716","creator":"system","date_created":"2018-12-12T10:08:53Z","file_name":"IST-2017-904-v1+1_elife-23136-v2.pdf","content_type":"application/pdf"},{"file_id":"4717","file_size":11242920,"date_updated":"2020-07-14T12:47:33Z","access_level":"open_access","checksum":"a6d542253028f52e00aa29739ddffe8f","relation":"main_file","file_name":"IST-2017-904-v1+2_elife-23136-figures-v2.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:08:54Z","creator":"system"}],"ddc":["579"],"has_accepted_license":"1","type":"journal_article","intvolume":" 6","oa_version":"Published Version","_id":"655","scopus_import":"1","publist_id":"7082","oa":1,"file_date_updated":"2020-07-14T12:47:33Z","isi":1,"department":[{"_id":"CaGu"}],"publisher":"eLife Sciences Publications","date_updated":"2025-09-11T07:11:19Z","language":[{"iso":"eng"}],"month":"03","date_published":"2017-03-06T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"pubrep_id":"904","status":"public","day":"06","citation":{"ama":"Renault T, Abraham A, Bergmiller T, et al. Bacterial flagella grow through an injection diffusion mechanism. eLife. 2017;6. doi:10.7554/eLife.23136","ista":"Renault T, Abraham A, Bergmiller T, Paradis G, Rainville S, Charpentier E, Guet CC, Tu Y, Namba K, Keener J, Minamino T, Erhardt M. 2017. Bacterial flagella grow through an injection diffusion mechanism. eLife. 6, e23136.","ieee":"T. Renault et al., “Bacterial flagella grow through an injection diffusion mechanism,” eLife, vol. 6. eLife Sciences Publications, 2017.","short":"T. Renault, A. Abraham, T. Bergmiller, G. Paradis, S. Rainville, E. Charpentier, C.C. Guet, Y. Tu, K. Namba, J. Keener, T. Minamino, M. Erhardt, ELife 6 (2017).","mla":"Renault, Thibaud, et al. “Bacterial Flagella Grow through an Injection Diffusion Mechanism.” ELife, vol. 6, e23136, eLife Sciences Publications, 2017, doi:10.7554/eLife.23136.","chicago":"Renault, Thibaud, Anthony Abraham, Tobias Bergmiller, Guillaume Paradis, Simon Rainville, Emmanuelle Charpentier, Calin C Guet, et al. “Bacterial Flagella Grow through an Injection Diffusion Mechanism.” ELife. eLife Sciences Publications, 2017. https://doi.org/10.7554/eLife.23136.","apa":"Renault, T., Abraham, A., Bergmiller, T., Paradis, G., Rainville, S., Charpentier, E., … Erhardt, M. (2017). Bacterial flagella grow through an injection diffusion mechanism. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.23136"}},{"article_processing_charge":"No","publication_status":"published","abstract":[{"text":"Human neurons transplanted into a mouse model for Alzheimer’s disease show human-specific vulnerability to β-amyloid plaques and may help to identify new therapeutic targets.","lang":"eng"}],"title":"Modeling Alzheimer's disease in mice with human neurons","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Novarino, Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178","last_name":"Novarino","first_name":"Gaia"}],"volume":9,"corr_author":"1","quality_controlled":"1","date_created":"2018-12-11T11:47:45Z","year":"2017","article_number":"eaam9867","publication_identifier":{"issn":["1946-6234"]},"doi":"10.1126/scitranslmed.aam9867","issue":"381","publication":"Science Translational Medicine","date_published":"2017-03-15T00:00:00Z","language":[{"iso":"eng"}],"month":"03","date_updated":"2025-07-10T11:53:32Z","publisher":"American Association for the Advancement of Science","department":[{"_id":"GaNo"}],"intvolume":" 9","oa_version":"None","type":"journal_article","publist_id":"7079","scopus_import":"1","_id":"656","citation":{"ieee":"G. Novarino, “Modeling Alzheimer’s disease in mice with human neurons,” Science Translational Medicine, vol. 9, no. 381. American Association for the Advancement of Science, 2017.","short":"G. Novarino, Science Translational Medicine 9 (2017).","ama":"Novarino G. Modeling Alzheimer’s disease in mice with human neurons. Science Translational Medicine. 2017;9(381). doi:10.1126/scitranslmed.aam9867","ista":"Novarino G. 2017. Modeling Alzheimer’s disease in mice with human neurons. Science Translational Medicine. 9(381), eaam9867.","apa":"Novarino, G. (2017). Modeling Alzheimer’s disease in mice with human neurons. Science Translational Medicine. American Association for the Advancement of Science. https://doi.org/10.1126/scitranslmed.aam9867","chicago":"Novarino, Gaia. “Modeling Alzheimer’s Disease in Mice with Human Neurons.” Science Translational Medicine. American Association for the Advancement of Science, 2017. https://doi.org/10.1126/scitranslmed.aam9867.","mla":"Novarino, Gaia. “Modeling Alzheimer’s Disease in Mice with Human Neurons.” Science Translational Medicine, vol. 9, no. 381, eaam9867, American Association for the Advancement of Science, 2017, doi:10.1126/scitranslmed.aam9867."},"status":"public","day":"15"},{"citation":{"mla":"Möller, Barbara, et al. “Auxin Response Cell Autonomously Controls Ground Tissue Initiation in the Early Arabidopsis Embryo.” PNAS, vol. 114, no. 12, National Academy of Sciences, 2017, pp. E2533–39, doi:10.1073/pnas.1616493114.","chicago":"Möller, Barbara, Colette Ten Hove, Daoquan Xiang, Nerys Williams, Lorena López, Saiko Yoshida, Margot Smit, Raju Datla, and Dolf Weijers. “Auxin Response Cell Autonomously Controls Ground Tissue Initiation in the Early Arabidopsis Embryo.” PNAS. National Academy of Sciences, 2017. https://doi.org/10.1073/pnas.1616493114.","apa":"Möller, B., Ten Hove, C., Xiang, D., Williams, N., López, L., Yoshida, S., … Weijers, D. (2017). Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1616493114","ista":"Möller B, Ten Hove C, Xiang D, Williams N, López L, Yoshida S, Smit M, Datla R, Weijers D. 2017. Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo. PNAS. 114(12), E2533–E2539.","ama":"Möller B, Ten Hove C, Xiang D, et al. Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo. PNAS. 2017;114(12):E2533-E2539. doi:10.1073/pnas.1616493114","ieee":"B. Möller et al., “Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo,” PNAS, vol. 114, no. 12. National Academy of Sciences, pp. E2533–E2539, 2017.","short":"B. Möller, C. Ten Hove, D. Xiang, N. Williams, L. López, S. Yoshida, M. Smit, R. Datla, D. Weijers, PNAS 114 (2017) E2533–E2539."},"status":"public","day":"21","date_published":"2017-03-21T00:00:00Z","language":[{"iso":"eng"}],"month":"03","date_updated":"2025-09-11T07:09:58Z","department":[{"_id":"JiFr"}],"publisher":"National Academy of Sciences","isi":1,"oa":1,"publist_id":"7076","scopus_import":"1","_id":"657","intvolume":" 114","oa_version":"Submitted Version","type":"journal_article","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373392/"}],"quality_controlled":"1","doi":"10.1073/pnas.1616493114","publication_identifier":{"issn":["0027-8424"]},"issue":"12","publication":"PNAS","date_created":"2018-12-11T11:47:45Z","year":"2017","title":"Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo","article_processing_charge":"No","page":"E2533 - E2539","publication_status":"published","external_id":{"pmid":["28265057"],"isi":["000396893600033"]},"abstract":[{"lang":"eng","text":"Plant organs are typically organized into three main tissue layers. The middle ground tissue layer comprises the majority of the plant body and serves a wide range of functions, including photosynthesis, selective nutrient uptake and storage, and gravity sensing. Ground tissue patterning and maintenance in Arabidopsis are controlled by a well-established gene network revolving around the key regulator SHORT-ROOT (SHR). In contrast, it is completely unknown how ground tissue identity is first specified from totipotent precursor cells in the embryo. The plant signaling molecule auxin, acting through AUXIN RESPONSE FACTOR (ARF) transcription factors, is critical for embryo patterning. The auxin effector ARF5/MONOPTEROS (MP) acts both cell-autonomously and noncell-autonomously to control embryonic vascular tissue formation and root initiation, respectively. Here we show that auxin response and ARF activity cell-autonomously control the asymmetric division of the first ground tissue cells. By identifying embryonic target genes, we show that MP transcriptionally initiates the ground tissue lineage and acts upstream of the regulatory network that controls ground tissue patterning and maintenance. Strikingly, whereas the SHR network depends on MP, this MP function is, at least in part, SHR independent. Our study therefore identifies auxin response as a regulator of ground tissue specification in the embryonic root, and reveals that ground tissue initiation and maintenance use different regulators and mechanisms. Moreover, our data provide a framework for the simultaneous formation of multiple cell types by the same transcriptional regulator."}],"pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"last_name":"Möller","first_name":"Barbara","full_name":"Möller, Barbara"},{"first_name":"Colette","last_name":"Ten Hove","full_name":"Ten Hove, Colette"},{"first_name":"Daoquan","last_name":"Xiang","full_name":"Xiang, Daoquan"},{"full_name":"Williams, Nerys","last_name":"Williams","first_name":"Nerys"},{"full_name":"López, Lorena","first_name":"Lorena","last_name":"López"},{"full_name":"Yoshida, Saiko","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko","last_name":"Yoshida"},{"last_name":"Smit","first_name":"Margot","full_name":"Smit, Margot"},{"full_name":"Datla, Raju","last_name":"Datla","first_name":"Raju"},{"full_name":"Weijers, Dolf","last_name":"Weijers","first_name":"Dolf"}],"volume":114},{"has_accepted_license":"1","ddc":["006"],"corr_author":"1","quality_controlled":"1","file":[{"file_id":"5371","access_level":"open_access","checksum":"b1bc43f96d1df3313c03032c2a46388d","relation":"main_file","date_updated":"2020-07-14T12:47:33Z","file_size":8439566,"date_created":"2018-12-12T10:18:49Z","file_name":"IST-2017-903-v1+1_fnbot-11-00008.pdf","content_type":"application/pdf","creator":"system"}],"doi":"10.3389/fnbot.2017.00008","publication_identifier":{"issn":["1662-5218"]},"issue":"MAR","publication":"Frontiers in Neurorobotics","date_created":"2018-12-11T11:47:45Z","year":"2017","article_number":"00008","title":"Self organized behavior generation for musculoskeletal robots","article_processing_charge":"Yes","external_id":{"isi":["000396303400001"]},"publication_status":"published","abstract":[{"text":"With the accelerated development of robot technologies, control becomes one of the central themes of research. In traditional approaches, the controller, by its internal functionality, finds appropriate actions on the basis of specific objectives for the task at hand. While very successful in many applications, self-organized control schemes seem to be favored in large complex systems with unknown dynamics or which are difficult to model. Reasons are the expected scalability, robustness, and resilience of self-organizing systems. The paper presents a self-learning neurocontroller based on extrinsic differential plasticity introduced recently, applying it to an anthropomorphic musculoskeletal robot arm with attached objects of unknown physical dynamics. The central finding of the paper is the following effect: by the mere feedback through the internal dynamics of the object, the robot is learning to relate each of the objects with a very specific sensorimotor pattern. Specifically, an attached pendulum pilots the arm into a circular motion, a half-filled bottle produces axis oriented shaking behavior, a wheel is getting rotated, and wiping patterns emerge automatically in a table-plus-brush setting. By these object-specific dynamical patterns, the robot may be said to recognize the object's identity, or in other words, it discovers dynamical affordances of objects. Furthermore, when including hand coordinates obtained from a camera, a dedicated hand-eye coordination self-organizes spontaneously. These phenomena are discussed from a specific dynamical system perspective. Central is the dedicated working regime at the border to instability with its potentially infinite reservoir of (limit cycle) attractors "waiting" to be excited. Besides converging toward one of these attractors, variate behavior is also arising from a self-induced attractor morphing driven by the learning rule. We claim that experimental investigations with this anthropomorphic, self-learning robot not only generate interesting and potentially useful behaviors, but may also help to better understand what subjective human muscle feelings are, how they can be rooted in sensorimotor patterns, and how these concepts may feed back on robotics.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"full_name":"Der, Ralf","first_name":"Ralf","last_name":"Der"},{"last_name":"Martius","first_name":"Georg S","id":"3A276B68-F248-11E8-B48F-1D18A9856A87","full_name":"Martius, Georg S"}],"volume":11,"citation":{"ista":"Der R, Martius GS. 2017. Self organized behavior generation for musculoskeletal robots. Frontiers in Neurorobotics. 11(MAR), 00008.","ama":"Der R, Martius GS. Self organized behavior generation for musculoskeletal robots. Frontiers in Neurorobotics. 2017;11(MAR). doi:10.3389/fnbot.2017.00008","short":"R. Der, G.S. Martius, Frontiers in Neurorobotics 11 (2017).","ieee":"R. Der and G. S. Martius, “Self organized behavior generation for musculoskeletal robots,” Frontiers in Neurorobotics, vol. 11, no. MAR. Frontiers Research Foundation, 2017.","mla":"Der, Ralf, and Georg S. Martius. “Self Organized Behavior Generation for Musculoskeletal Robots.” Frontiers in Neurorobotics, vol. 11, no. MAR, 00008, Frontiers Research Foundation, 2017, doi:10.3389/fnbot.2017.00008.","chicago":"Der, Ralf, and Georg S Martius. “Self Organized Behavior Generation for Musculoskeletal Robots.” Frontiers in Neurorobotics. Frontiers Research Foundation, 2017. https://doi.org/10.3389/fnbot.2017.00008.","apa":"Der, R., & Martius, G. S. (2017). Self organized behavior generation for musculoskeletal robots. Frontiers in Neurorobotics. Frontiers Research Foundation. https://doi.org/10.3389/fnbot.2017.00008"},"project":[{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"}],"pubrep_id":"903","day":"16","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ec_funded":1,"date_published":"2017-03-16T00:00:00Z","language":[{"iso":"eng"}],"month":"03","date_updated":"2025-09-11T07:10:33Z","department":[{"_id":"ChLa"},{"_id":"GaTk"}],"publisher":"Frontiers Research Foundation","isi":1,"file_date_updated":"2020-07-14T12:47:33Z","oa":1,"publist_id":"7078","scopus_import":"1","_id":"658","intvolume":" 11","oa_version":"Published Version","type":"journal_article"},{"doi":"10.1038/ncomms14832","publication_identifier":{"issn":["2041-1723"]},"publication":"Nature Communications","date_created":"2018-12-11T11:47:46Z","year":"2017","article_number":"14832","has_accepted_license":"1","ddc":["570"],"quality_controlled":"1","file":[{"date_updated":"2020-07-14T12:47:34Z","file_size":9523746,"relation":"main_file","checksum":"dae30190291c3630e8102d8714a8d23e","access_level":"open_access","file_id":"5072","creator":"system","file_name":"IST-2017-902-v1+1_Kage_et_al-2017-Nature_Communications.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:14:21Z"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"full_name":"Kage, Frieda","first_name":"Frieda","last_name":"Kage"},{"full_name":"Winterhoff, Moritz","last_name":"Winterhoff","first_name":"Moritz"},{"first_name":"Vanessa","last_name":"Dimchev","full_name":"Dimchev, Vanessa"},{"id":"AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D","full_name":"Müller, Jan","last_name":"Müller","first_name":"Jan"},{"full_name":"Thalheim, Tobias","last_name":"Thalheim","first_name":"Tobias"},{"full_name":"Freise, Anika","last_name":"Freise","first_name":"Anika"},{"first_name":"Stefan","last_name":"Brühmann","full_name":"Brühmann, Stefan"},{"first_name":"Jana","last_name":"Kollasser","full_name":"Kollasser, Jana"},{"full_name":"Block, Jennifer","first_name":"Jennifer","last_name":"Block"},{"last_name":"Dimchev","first_name":"Georgi A","full_name":"Dimchev, Georgi A"},{"full_name":"Geyer, Matthias","first_name":"Matthias","last_name":"Geyer"},{"full_name":"Schnittler, Hams","last_name":"Schnittler","first_name":"Hams"},{"full_name":"Brakebusch, Cord","first_name":"Cord","last_name":"Brakebusch"},{"full_name":"Stradal, Theresia","first_name":"Theresia","last_name":"Stradal"},{"last_name":"Carlier","first_name":"Marie","full_name":"Carlier, Marie"},{"last_name":"Sixt","first_name":"Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"},{"full_name":"Käs, Josef","first_name":"Josef","last_name":"Käs"},{"full_name":"Faix, Jan","last_name":"Faix","first_name":"Jan"},{"first_name":"Klemens","last_name":"Rottner","full_name":"Rottner, Klemens"}],"volume":8,"title":"FMNL formins boost lamellipodial force generation","article_processing_charge":"No","external_id":{"isi":["000396993700001"]},"publication_status":"published","abstract":[{"text":"Migration frequently involves Rac-mediated protrusion of lamellipodia, formed by Arp2/3 complex-dependent branching thought to be crucial for force generation and stability of these networks. The formins FMNL2 and FMNL3 are Cdc42 effectors targeting to the lamellipodium tip and shown here to nucleate and elongate actin filaments with complementary activities in vitro. In migrating B16-F1 melanoma cells, both formins contribute to the velocity of lamellipodium protrusion. Loss of FMNL2/3 function in melanoma cells and fibroblasts reduces lamellipodial width, actin filament density and -bundling, without changing patterns of Arp2/3 complex incorporation. Strikingly, in melanoma cells, FMNL2/3 gene inactivation almost completely abolishes protrusion forces exerted by lamellipodia and modifies their ultrastructural organization. Consistently, CRISPR/Cas-mediated depletion of FMNL2/3 in fibroblasts reduces both migration and capability of cells to move against viscous media. Together, we conclude that force generation in lamellipodia strongly depends on FMNL formin activity, operating in addition to Arp2/3 complex-dependent filament branching.","lang":"eng"}],"day":"22","pubrep_id":"902","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"apa":"Kage, F., Winterhoff, M., Dimchev, V., Müller, J., Thalheim, T., Freise, A., … Rottner, K. (2017). FMNL formins boost lamellipodial force generation. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms14832","chicago":"Kage, Frieda, Moritz Winterhoff, Vanessa Dimchev, Jan Müller, Tobias Thalheim, Anika Freise, Stefan Brühmann, et al. “FMNL Formins Boost Lamellipodial Force Generation.” Nature Communications. Nature Publishing Group, 2017. https://doi.org/10.1038/ncomms14832.","mla":"Kage, Frieda, et al. “FMNL Formins Boost Lamellipodial Force Generation.” Nature Communications, vol. 8, 14832, Nature Publishing Group, 2017, doi:10.1038/ncomms14832.","short":"F. Kage, M. Winterhoff, V. Dimchev, J. Müller, T. Thalheim, A. Freise, S. Brühmann, J. Kollasser, J. Block, G.A. Dimchev, M. Geyer, H. Schnittler, C. Brakebusch, T. Stradal, M. Carlier, M.K. Sixt, J. Käs, J. Faix, K. Rottner, Nature Communications 8 (2017).","ieee":"F. Kage et al., “FMNL formins boost lamellipodial force generation,” Nature Communications, vol. 8. Nature Publishing Group, 2017.","ista":"Kage F, Winterhoff M, Dimchev V, Müller J, Thalheim T, Freise A, Brühmann S, Kollasser J, Block J, Dimchev GA, Geyer M, Schnittler H, Brakebusch C, Stradal T, Carlier M, Sixt MK, Käs J, Faix J, Rottner K. 2017. FMNL formins boost lamellipodial force generation. Nature Communications. 8, 14832.","ama":"Kage F, Winterhoff M, Dimchev V, et al. FMNL formins boost lamellipodial force generation. Nature Communications. 2017;8. doi:10.1038/ncomms14832"},"publist_id":"7075","scopus_import":"1","_id":"659","oa_version":"Published Version","intvolume":" 8","type":"journal_article","date_published":"2017-03-22T00:00:00Z","language":[{"iso":"eng"}],"month":"03","date_updated":"2025-09-11T07:09:28Z","department":[{"_id":"MiSi"}],"publisher":"Nature Publishing Group","isi":1,"file_date_updated":"2020-07-14T12:47:34Z","oa":1},{"page":"3427 - 3432","article_processing_charge":"No","abstract":[{"text":"Growing microtubules are protected from depolymerization by the presence of a GTP or GDP/Pi cap. End-binding proteins of the EB1 family bind to the stabilizing cap, allowing monitoring of its size in real time. The cap size has been shown to correlate with instantaneous microtubule stability. Here we have quantitatively characterized the properties of cap size fluctuations during steadystate growth and have developed a theory predicting their timescale and amplitude from the kinetics of microtubule growth and cap maturation. In contrast to growth speed fluctuations, cap size fluctuations show a characteristic timescale, which is defined by the lifetime of the cap sites. Growth fluctuations affect the amplitude of cap size fluctuations; however, cap size does not affect growth speed, indicating that microtubules are far from instability during most of their time of growth. Our theory provides the basis for a quantitative understanding of microtubule stability fluctuations during steady-state growth.","lang":"eng"}],"publication_status":"published","external_id":{"pmid":["28280102"],"isi":["000397607300065"]},"title":"Steady state EB cap size fluctuations are determined by stochastic microtubule growth and maturation","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"first_name":"Jamie","last_name":"Rickman","full_name":"Rickman, Jamie"},{"orcid":"0000-0001-6335-9748","id":"459064DC-F248-11E8-B48F-1D18A9856A87","full_name":"Düllberg, Christian F","first_name":"Christian F","last_name":"Düllberg"},{"first_name":"Nicholas","last_name":"Cade","full_name":"Cade, Nicholas"},{"full_name":"Griffin, Lewis","first_name":"Lewis","last_name":"Griffin"},{"full_name":"Surrey, Thomas","first_name":"Thomas","last_name":"Surrey"}],"volume":114,"pmid":1,"quality_controlled":"1","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380103/","open_access":"1"}],"year":"2017","date_created":"2018-12-11T11:47:46Z","issue":"13","publication_identifier":{"issn":["0027-8424"]},"doi":"10.1073/pnas.1620274114","publication":"PNAS","isi":1,"oa":1,"language":[{"iso":"eng"}],"month":"03","date_published":"2017-03-28T00:00:00Z","department":[{"_id":"MaLo"}],"publisher":"National Academy of Sciences","date_updated":"2025-09-11T07:08:20Z","oa_version":"Submitted Version","intvolume":" 114","type":"journal_article","acknowledgement":"We thank Philippe Cluzel for helpful discussions and Gunnar Pruessner for data analysis advice. This work was supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK Grant FC001163, Medical Research Council Grant FC001163, and Wellcome Trust Grant FC001163. This work was also supported by European Research Council Advanced Grant Project 323042 (to C.D. and T.S.).","publist_id":"7073","_id":"660","scopus_import":"1","citation":{"ieee":"J. Rickman, C. F. Düllberg, N. Cade, L. Griffin, and T. Surrey, “Steady state EB cap size fluctuations are determined by stochastic microtubule growth and maturation,” PNAS, vol. 114, no. 13. National Academy of Sciences, pp. 3427–3432, 2017.","short":"J. Rickman, C.F. Düllberg, N. Cade, L. Griffin, T. Surrey, PNAS 114 (2017) 3427–3432.","ama":"Rickman J, Düllberg CF, Cade N, Griffin L, Surrey T. Steady state EB cap size fluctuations are determined by stochastic microtubule growth and maturation. PNAS. 2017;114(13):3427-3432. doi:10.1073/pnas.1620274114","ista":"Rickman J, Düllberg CF, Cade N, Griffin L, Surrey T. 2017. Steady state EB cap size fluctuations are determined by stochastic microtubule growth and maturation. PNAS. 114(13), 3427–3432.","apa":"Rickman, J., Düllberg, C. F., Cade, N., Griffin, L., & Surrey, T. (2017). Steady state EB cap size fluctuations are determined by stochastic microtubule growth and maturation. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1620274114","chicago":"Rickman, Jamie, Christian F Düllberg, Nicholas Cade, Lewis Griffin, and Thomas Surrey. “Steady State EB Cap Size Fluctuations Are Determined by Stochastic Microtubule Growth and Maturation.” PNAS. National Academy of Sciences, 2017. https://doi.org/10.1073/pnas.1620274114.","mla":"Rickman, Jamie, et al. “Steady State EB Cap Size Fluctuations Are Determined by Stochastic Microtubule Growth and Maturation.” PNAS, vol. 114, no. 13, National Academy of Sciences, 2017, pp. 3427–32, doi:10.1073/pnas.1620274114."},"day":"28","status":"public"},{"quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1703.01714"}],"article_number":"044107","year":"2017","date_created":"2018-12-11T11:47:47Z","publication":"Physics of Fluids","issue":"4","doi":"10.1063/1.4981525","publication_identifier":{"issn":["1070-6631"]},"abstract":[{"text":"We report a direct-numerical-simulation study of the Taylor-Couette flow in the quasi-Keplerian regime at shear Reynolds numbers up to (105). Quasi-Keplerian rotating flow has been investigated for decades as a simplified model system to study the origin of turbulence in accretion disks that is not fully understood. The flow in this study is axially periodic and thus the experimental end-wall effects on the stability of the flow are avoided. Using optimal linear perturbations as initial conditions, our simulations find no sustained turbulence: the strong initial perturbations distort the velocity profile and trigger turbulence that eventually decays.","lang":"eng"}],"publication_status":"published","external_id":{"isi":["000400384100022"],"arxiv":["1703.01714"]},"article_processing_charge":"No","title":"Hydrodynamic turbulence in quasi Keplerian rotating flows","volume":29,"author":[{"full_name":"Shi, Liang","last_name":"Shi","first_name":"Liang"},{"last_name":"Hof","first_name":"Björn","orcid":"0000-0003-2057-2754","id":"3A374330-F248-11E8-B48F-1D18A9856A87","full_name":"Hof, Björn"},{"full_name":"Rampp, Markus","last_name":"Rampp","first_name":"Markus"},{"last_name":"Avila","first_name":"Marc","full_name":"Avila, Marc"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","project":[{"_id":"2511D90C-B435-11E9-9278-68D0E5697425","grant_number":"SFB 963 TP A8","name":"Astrophysical instability of currents and turbulences"}],"citation":{"ama":"Shi L, Hof B, Rampp M, Avila M. Hydrodynamic turbulence in quasi Keplerian rotating flows. Physics of Fluids. 2017;29(4). doi:10.1063/1.4981525","ista":"Shi L, Hof B, Rampp M, Avila M. 2017. Hydrodynamic turbulence in quasi Keplerian rotating flows. Physics of Fluids. 29(4), 044107.","ieee":"L. Shi, B. Hof, M. Rampp, and M. Avila, “Hydrodynamic turbulence in quasi Keplerian rotating flows,” Physics of Fluids, vol. 29, no. 4. American Institute of Physics, 2017.","short":"L. Shi, B. Hof, M. Rampp, M. Avila, Physics of Fluids 29 (2017).","mla":"Shi, Liang, et al. “Hydrodynamic Turbulence in Quasi Keplerian Rotating Flows.” Physics of Fluids, vol. 29, no. 4, 044107, American Institute of Physics, 2017, doi:10.1063/1.4981525.","chicago":"Shi, Liang, Björn Hof, Markus Rampp, and Marc Avila. “Hydrodynamic Turbulence in Quasi Keplerian Rotating Flows.” Physics of Fluids. American Institute of Physics, 2017. https://doi.org/10.1063/1.4981525.","apa":"Shi, L., Hof, B., Rampp, M., & Avila, M. (2017). Hydrodynamic turbulence in quasi Keplerian rotating flows. Physics of Fluids. American Institute of Physics. https://doi.org/10.1063/1.4981525"},"day":"01","status":"public","oa":1,"isi":1,"department":[{"_id":"BjHo"}],"publisher":"American Institute of Physics","arxiv":1,"date_updated":"2025-09-11T07:06:55Z","language":[{"iso":"eng"}],"month":"04","date_published":"2017-04-01T00:00:00Z","type":"journal_article","intvolume":" 29","oa_version":"Submitted Version","_id":"662","scopus_import":"1","publist_id":"7072"},{"citation":{"ama":"Kong H, Bogomolov S, Schilling C, Jiang Y, Henzinger TA. Safety verification of nonlinear hybrid systems based on invariant clusters. In: Proceedings of the 20th International Conference on Hybrid Systems. ACM; 2017:163-172. doi:10.1145/3049797.3049814","ista":"Kong H, Bogomolov S, Schilling C, Jiang Y, Henzinger TA. 2017. Safety verification of nonlinear hybrid systems based on invariant clusters. Proceedings of the 20th International Conference on Hybrid Systems. HSCC: Hybrid Systems - Computation and Control , 163–172.","ieee":"H. Kong, S. Bogomolov, C. Schilling, Y. Jiang, and T. A. Henzinger, “Safety verification of nonlinear hybrid systems based on invariant clusters,” in Proceedings of the 20th International Conference on Hybrid Systems, Pittsburgh, PA, United States, 2017, pp. 163–172.","short":"H. Kong, S. Bogomolov, C. Schilling, Y. Jiang, T.A. Henzinger, in:, Proceedings of the 20th International Conference on Hybrid Systems, ACM, 2017, pp. 163–172.","mla":"Kong, Hui, et al. “Safety Verification of Nonlinear Hybrid Systems Based on Invariant Clusters.” Proceedings of the 20th International Conference on Hybrid Systems, ACM, 2017, pp. 163–72, doi:10.1145/3049797.3049814.","chicago":"Kong, Hui, Sergiy Bogomolov, Christian Schilling, Yu Jiang, and Thomas A Henzinger. “Safety Verification of Nonlinear Hybrid Systems Based on Invariant Clusters.” In Proceedings of the 20th International Conference on Hybrid Systems, 163–72. ACM, 2017. https://doi.org/10.1145/3049797.3049814.","apa":"Kong, H., Bogomolov, S., Schilling, C., Jiang, Y., & Henzinger, T. A. (2017). Safety verification of nonlinear hybrid systems based on invariant clusters. In Proceedings of the 20th International Conference on Hybrid Systems (pp. 163–172). Pittsburgh, PA, United States: ACM. https://doi.org/10.1145/3049797.3049814"},"status":"public","day":"01","pubrep_id":"817","isi":1,"oa":1,"file_date_updated":"2020-07-14T12:47:34Z","language":[{"iso":"eng"}],"month":"04","date_published":"2017-04-01T00:00:00Z","publisher":"ACM","department":[{"_id":"ToHe"}],"date_updated":"2025-09-11T07:06:15Z","oa_version":"Submitted Version","type":"conference","publist_id":"7067","_id":"663","scopus_import":"1","file":[{"file_id":"4873","file_size":1650530,"date_updated":"2020-07-14T12:47:34Z","relation":"main_file","checksum":"b7667434cbf5b5f0ade3bea1dbe5bf63","access_level":"open_access","file_name":"IST-2017-817-v1+1_p163-kong.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:11:20Z","creator":"system"}],"quality_controlled":"1","has_accepted_license":"1","conference":{"location":"Pittsburgh, PA, United States","end_date":"2017-04-20","start_date":"2017-04-18","name":"HSCC: Hybrid Systems - Computation and Control "},"ddc":["000"],"year":"2017","date_created":"2018-12-11T11:47:47Z","doi":"10.1145/3049797.3049814","publication_identifier":{"isbn":["978-145034590-3"]},"publication":"Proceedings of the 20th International Conference on Hybrid Systems","page":"163 - 172","article_processing_charge":"No","abstract":[{"text":"In this paper, we propose an approach to automatically compute invariant clusters for nonlinear semialgebraic hybrid systems. An invariant cluster for an ordinary differential equation (ODE) is a multivariate polynomial invariant g(u→, x→) = 0, parametric in u→, which can yield an infinite number of concrete invariants by assigning different values to u→ so that every trajectory of the system can be overapproximated precisely by the intersection of a group of concrete invariants. For semialgebraic systems, which involve ODEs with multivariate polynomial right-hand sides, given a template multivariate polynomial g(u→, x→), an invariant cluster can be obtained by first computing the remainder of the Lie derivative of g(u→, x→) divided by g(u→, x→) and then solving the system of polynomial equations obtained from the coefficients of the remainder. Based on invariant clusters and sum-of-squares (SOS) programming, we present a new method for the safety verification of hybrid systems. Experiments on nonlinear benchmark systems from biology and control theory show that our approach is efficient. ","lang":"eng"}],"publication_status":"published","external_id":{"isi":["000615962400019"]},"title":"Safety verification of nonlinear hybrid systems based on invariant clusters","author":[{"orcid":"0000-0002-3066-6941","id":"3BDE25AA-F248-11E8-B48F-1D18A9856A87","full_name":"Kong, Hui","last_name":"Kong","first_name":"Hui"},{"first_name":"Sergiy","last_name":"Bogomolov","orcid":"0000-0002-0686-0365","full_name":"Bogomolov, Sergiy"},{"first_name":"Christian","last_name":"Schilling","full_name":"Schilling, Christian"},{"last_name":"Jiang","first_name":"Yu","full_name":"Jiang, Yu"},{"orcid":"0000−0002−2985−7724","id":"40876CD8-F248-11E8-B48F-1D18A9856A87","full_name":"Henzinger, Thomas A","first_name":"Thomas A","last_name":"Henzinger"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"quality_controlled":"1","corr_author":"1","year":"2017","date_created":"2018-12-11T11:47:48Z","publication":"Science","issue":"6335","publication_identifier":{"issn":["0036-8075"]},"doi":"10.1126/science.aaf4762","abstract":[{"lang":"eng","text":"The molecular mechanisms underlying phenotypic variation in isogenic bacterial populations remain poorly understood.We report that AcrAB-TolC, the main multidrug efflux pump of Escherichia coli, exhibits a strong partitioning bias for old cell poles by a segregation mechanism that is mediated by ternary AcrAB-TolC complex formation. Mother cells inheriting old poles are phenotypically distinct and display increased drug efflux activity relative to daughters. Consequently, we find systematic and long-lived growth differences between mother and daughter cells in the presence of subinhibitory drug concentrations. A simple model for biased partitioning predicts a population structure of long-lived and highly heterogeneous phenotypes. This straightforward mechanism of generating sustained growth rate differences at subinhibitory antibiotic concentrations has implications for understanding the emergence of multidrug resistance in bacteria."}],"external_id":{"isi":["000399540100060"]},"publication_status":"published","page":"311 - 315","article_processing_charge":"No","title":"Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity","volume":356,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"id":"2C471CFA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5396-4346","full_name":"Bergmiller, Tobias","last_name":"Bergmiller","first_name":"Tobias"},{"full_name":"Andersson, Anna M","orcid":"0000-0003-2912-6769","id":"2B8A40DA-F248-11E8-B48F-1D18A9856A87","last_name":"Andersson","first_name":"Anna M"},{"first_name":"Kathrin","last_name":"Tomasek","orcid":"0000-0003-3768-877X","id":"3AEC8556-F248-11E8-B48F-1D18A9856A87","full_name":"Tomasek, Kathrin"},{"full_name":"Balleza, Enrique","first_name":"Enrique","last_name":"Balleza"},{"first_name":"Daniel","last_name":"Kiviet","full_name":"Kiviet, Daniel"},{"first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"orcid":"0000-0002-6699-1455","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","full_name":"Tkacik, Gasper","last_name":"Tkacik","first_name":"Gasper"},{"full_name":"Guet, Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6220-2052","first_name":"Calin C","last_name":"Guet"}],"related_material":{"record":[{"id":"5560","relation":"popular_science","status":"public"}]},"project":[{"call_identifier":"FWF","name":"Biophysics of information processing in gene regulation","_id":"254E9036-B435-11E9-9278-68D0E5697425","grant_number":"P28844-B27"}],"citation":{"short":"T. Bergmiller, A.M. Andersson, K. Tomasek, E. Balleza, D. Kiviet, R. Hauschild, G. Tkačik, C.C. Guet, Science 356 (2017) 311–315.","ieee":"T. Bergmiller et al., “Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity,” Science, vol. 356, no. 6335. American Association for the Advancement of Science, pp. 311–315, 2017.","ista":"Bergmiller T, Andersson AM, Tomasek K, Balleza E, Kiviet D, Hauschild R, Tkačik G, Guet CC. 2017. Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. Science. 356(6335), 311–315.","ama":"Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. Science. 2017;356(6335):311-315. doi:10.1126/science.aaf4762","apa":"Bergmiller, T., Andersson, A. M., Tomasek, K., Balleza, E., Kiviet, D., Hauschild, R., … Guet, C. C. (2017). Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.aaf4762","chicago":"Bergmiller, Tobias, Anna M Andersson, Kathrin Tomasek, Enrique Balleza, Daniel Kiviet, Robert Hauschild, Gašper Tkačik, and Calin C Guet. “Biased Partitioning of the Multidrug Efflux Pump AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.” Science. American Association for the Advancement of Science, 2017. https://doi.org/10.1126/science.aaf4762.","mla":"Bergmiller, Tobias, et al. “Biased Partitioning of the Multidrug Efflux Pump AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.” Science, vol. 356, no. 6335, American Association for the Advancement of Science, 2017, pp. 311–15, doi:10.1126/science.aaf4762."},"status":"public","day":"21","isi":1,"publisher":"American Association for the Advancement of Science","department":[{"_id":"CaGu"},{"_id":"GaTk"},{"_id":"Bio"}],"date_updated":"2025-09-11T07:05:04Z","language":[{"iso":"eng"}],"month":"04","date_published":"2017-04-21T00:00:00Z","type":"journal_article","article_type":"original","intvolume":" 356","oa_version":"None","_id":"665","scopus_import":"1","publist_id":"7064"},{"volume":4,"author":[{"full_name":"Mitosch, Karin","id":"39B66846-F248-11E8-B48F-1D18A9856A87","last_name":"Mitosch","first_name":"Karin"},{"id":"34DA8BD6-F248-11E8-B48F-1D18A9856A87","full_name":"Rieckh, Georg","first_name":"Georg","last_name":"Rieckh"},{"full_name":"Bollenbach, Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4398-476X","first_name":"Tobias","last_name":"Bollenbach"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","related_material":{"record":[{"relation":"dissertation_contains","id":"818","status":"public"}]},"abstract":[{"lang":"eng","text":"Antibiotics elicit drastic changes in microbial gene expression, including the induction of stress response genes. While certain stress responses are known to “cross-protect” bacteria from other stressors, it is unclear whether cellular responses to antibiotics have a similar protective role. By measuring the genome-wide transcriptional response dynamics of Escherichia coli to four antibiotics, we found that trimethoprim induces a rapid acid stress response that protects bacteria from subsequent exposure to acid. Combining microfluidics with time-lapse imaging to monitor survival and acid stress response in single cells revealed that the noisy expression of the acid resistance operon gadBC correlates with single-cell survival. Cells with higher gadBC expression following trimethoprim maintain higher intracellular pH and survive the acid stress longer. The seemingly random single-cell survival under acid stress can therefore be predicted from gadBC expression and rationalized in terms of GadB/C molecular function. Overall, we provide a roadmap for identifying the molecular mechanisms of single-cell cross-protection between antibiotics and other stressors."}],"external_id":{"isi":["000402747300005"]},"publication_status":"published","page":"393 - 403","article_processing_charge":"Yes (in subscription journal)","title":"Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment","year":"2017","date_created":"2018-12-11T11:47:48Z","publication":"Cell Systems","issue":"4","publication_identifier":{"issn":["2405-4712"]},"doi":"10.1016/j.cels.2017.03.001","file":[{"content_type":"application/pdf","file_name":"IST-2017-901-v1+1_1-s2.0-S2405471217300868-main.pdf","date_created":"2018-12-12T10:13:54Z","creator":"system","file_id":"5041","file_size":2438660,"date_updated":"2020-07-14T12:47:35Z","access_level":"open_access","relation":"main_file","checksum":"04ff20011c3d9a601c514aa999a5fe1a"}],"quality_controlled":"1","corr_author":"1","ddc":["576","610"],"has_accepted_license":"1","type":"journal_article","oa_version":"Published Version","intvolume":" 4","_id":"666","scopus_import":"1","publist_id":"7061","oa":1,"file_date_updated":"2020-07-14T12:47:35Z","isi":1,"department":[{"_id":"ToBo"},{"_id":"GaTk"}],"publisher":"Cell Press","date_updated":"2026-04-08T14:21:56Z","language":[{"iso":"eng"}],"month":"04","date_published":"2017-04-26T00:00:00Z","ec_funded":1,"tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"day":"26","pubrep_id":"901","status":"public","project":[{"call_identifier":"FP7","name":"Optimality principles in responses to antibiotics","_id":"25E83C2C-B435-11E9-9278-68D0E5697425","grant_number":"303507"},{"name":"Revealing the mechanisms underlying drug interactions","call_identifier":"FWF","grant_number":"P27201-B22","_id":"25E9AF9E-B435-11E9-9278-68D0E5697425"},{"grant_number":"RGP0042/2013","_id":"25EB3A80-B435-11E9-9278-68D0E5697425","name":"Revealing the fundamental limits of cell growth"}],"citation":{"ieee":"K. Mitosch, G. Rieckh, and M. T. Bollenbach, “Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment,” Cell Systems, vol. 4, no. 4. Cell Press, pp. 393–403, 2017.","short":"K. Mitosch, G. Rieckh, M.T. Bollenbach, Cell Systems 4 (2017) 393–403.","ista":"Mitosch K, Rieckh G, Bollenbach MT. 2017. Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment. Cell Systems. 4(4), 393–403.","ama":"Mitosch K, Rieckh G, Bollenbach MT. Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment. Cell Systems. 2017;4(4):393-403. doi:10.1016/j.cels.2017.03.001","apa":"Mitosch, K., Rieckh, G., & Bollenbach, M. T. (2017). Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment. Cell Systems. Cell Press. https://doi.org/10.1016/j.cels.2017.03.001","chicago":"Mitosch, Karin, Georg Rieckh, and Mark Tobias Bollenbach. “Noisy Response to Antibiotic Stress Predicts Subsequent Single Cell Survival in an Acidic Environment.” Cell Systems. Cell Press, 2017. https://doi.org/10.1016/j.cels.2017.03.001.","mla":"Mitosch, Karin, et al. “Noisy Response to Antibiotic Stress Predicts Subsequent Single Cell Survival in an Acidic Environment.” Cell Systems, vol. 4, no. 4, Cell Press, 2017, pp. 393–403, doi:10.1016/j.cels.2017.03.001."}},{"corr_author":"1","quality_controlled":"1","issue":"387","publication_identifier":{"issn":["1946-6234"]},"doi":"10.1126/scitranslmed.aan2786","publication":"Science Translational Medicine","year":"2017","date_created":"2018-12-11T11:47:48Z","article_number":"2786","title":"The antisocial side of antibiotics","article_processing_charge":"No","abstract":[{"text":"Perinatal exposure to penicillin may result in longlasting gut and behavioral changes.","lang":"eng"}],"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Novarino","first_name":"Gaia","full_name":"Novarino, Gaia","orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87"}],"volume":9,"citation":{"short":"G. Novarino, Science Translational Medicine 9 (2017).","ieee":"G. Novarino, “The antisocial side of antibiotics,” Science Translational Medicine, vol. 9, no. 387. American Association for the Advancement of Science, 2017.","ama":"Novarino G. The antisocial side of antibiotics. Science Translational Medicine. 2017;9(387). doi:10.1126/scitranslmed.aan2786","ista":"Novarino G. 2017. The antisocial side of antibiotics. Science Translational Medicine. 9(387), 2786.","apa":"Novarino, G. (2017). The antisocial side of antibiotics. Science Translational Medicine. American Association for the Advancement of Science. https://doi.org/10.1126/scitranslmed.aan2786","chicago":"Novarino, Gaia. “The Antisocial Side of Antibiotics.” Science Translational Medicine. American Association for the Advancement of Science, 2017. https://doi.org/10.1126/scitranslmed.aan2786.","mla":"Novarino, Gaia. “The Antisocial Side of Antibiotics.” Science Translational Medicine, vol. 9, no. 387, 2786, American Association for the Advancement of Science, 2017, doi:10.1126/scitranslmed.aan2786."},"day":"26","status":"public","language":[{"iso":"eng"}],"month":"04","date_published":"2017-04-26T00:00:00Z","publisher":"American Association for the Advancement of Science","department":[{"_id":"GaNo"}],"date_updated":"2025-07-10T11:53:44Z","publist_id":"7060","_id":"667","scopus_import":"1","oa_version":"None","intvolume":" 9","type":"journal_article"},{"type":"journal_article","article_type":"original","intvolume":" 292","oa_version":"Published Version","_id":"668","scopus_import":"1","publist_id":"7059","oa":1,"file_date_updated":"2020-07-14T12:47:37Z","isi":1,"department":[{"_id":"MiSi"}],"publisher":"American Society for Biochemistry and Molecular Biology","date_updated":"2025-09-11T07:03:17Z","language":[{"iso":"eng"}],"month":"04","date_published":"2017-04-28T00:00:00Z","status":"public","day":"28","citation":{"apa":"Horsthemke, M., Bachg, A., Groll, K., Moyzio, S., Müther, B., Hemkemeyer, S., … Hanley, P. (2017). Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion. Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology. https://doi.org/10.1074/jbc.M116.766923","chicago":"Horsthemke, Markus, Anne Bachg, Katharina Groll, Sven Moyzio, Barbara Müther, Sandra Hemkemeyer, Roland Wedlich Söldner, et al. “Multiple Roles of Filopodial Dynamics in Particle Capture and Phagocytosis and Phenotypes of Cdc42 and Myo10 Deletion.” Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 2017. https://doi.org/10.1074/jbc.M116.766923.","mla":"Horsthemke, Markus, et al. “Multiple Roles of Filopodial Dynamics in Particle Capture and Phagocytosis and Phenotypes of Cdc42 and Myo10 Deletion.” Journal of Biological Chemistry, vol. 292, no. 17, American Society for Biochemistry and Molecular Biology, 2017, pp. 7258–73, doi:10.1074/jbc.M116.766923.","ieee":"M. Horsthemke et al., “Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion,” Journal of Biological Chemistry, vol. 292, no. 17. American Society for Biochemistry and Molecular Biology, pp. 7258–7273, 2017.","short":"M. Horsthemke, A. Bachg, K. Groll, S. Moyzio, B. Müther, S. Hemkemeyer, R. Wedlich Söldner, M.K. Sixt, S. Tacke, M. Bähler, P. Hanley, Journal of Biological Chemistry 292 (2017) 7258–7273.","ama":"Horsthemke M, Bachg A, Groll K, et al. Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion. Journal of Biological Chemistry. 2017;292(17):7258-7273. doi:10.1074/jbc.M116.766923","ista":"Horsthemke M, Bachg A, Groll K, Moyzio S, Müther B, Hemkemeyer S, Wedlich Söldner R, Sixt MK, Tacke S, Bähler M, Hanley P. 2017. Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion. Journal of Biological Chemistry. 292(17), 7258–7273."},"volume":292,"author":[{"full_name":"Horsthemke, Markus","first_name":"Markus","last_name":"Horsthemke"},{"full_name":"Bachg, Anne","first_name":"Anne","last_name":"Bachg"},{"first_name":"Katharina","last_name":"Groll","full_name":"Groll, Katharina"},{"first_name":"Sven","last_name":"Moyzio","full_name":"Moyzio, Sven"},{"first_name":"Barbara","last_name":"Müther","full_name":"Müther, Barbara"},{"full_name":"Hemkemeyer, Sandra","last_name":"Hemkemeyer","first_name":"Sandra"},{"full_name":"Wedlich Söldner, Roland","last_name":"Wedlich Söldner","first_name":"Roland"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K"},{"full_name":"Tacke, Sebastian","first_name":"Sebastian","last_name":"Tacke"},{"full_name":"Bähler, Martin","first_name":"Martin","last_name":"Bähler"},{"full_name":"Hanley, Peter","last_name":"Hanley","first_name":"Peter"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"text":"Macrophage filopodia, finger-like membrane protrusions, were first implicated in phagocytosis more than 100 years ago, but little is still known about the involvement of these actin-dependent structures in particle clearance. Using spinning disk confocal microscopy to image filopodial dynamics in mouse resident Lifeact-EGFP macrophages, we show that filopodia, or filopodia-like structures, support pathogen clearance by multiple means. Filopodia supported the phagocytic uptake of bacterial (Escherichia coli) particles by (i) capturing along the filopodial shaft and surfing toward the cell body, the most common mode of capture; (ii) capturing via the tip followed by retraction; (iii) combinations of surfing and retraction; or (iv) sweeping actions. In addition, filopodia supported the uptake of zymosan (Saccharomyces cerevisiae) particles by (i) providing fixation, (ii) capturing at the tip and filopodia-guided actin anterograde flow with phagocytic cup formation, and (iii) the rapid growth of new protrusions. To explore the role of filopodia-inducing Cdc42, we generated myeloid-restricted Cdc42 knock-out mice. Cdc42-deficient macrophages exhibited rapid phagocytic cup kinetics, but reduced particle clearance, which could be explained by the marked rounded-up morphology of these cells. Macrophages lacking Myo10, thought to act downstream of Cdc42, had normal morphology, motility, and phagocytic cup formation, but displayed markedly reduced filopodia formation. In conclusion, live-cell imaging revealed multiple mechanisms involving macrophage filopodia in particle capture and engulfment. Cdc42 is not critical for filopodia or phagocytic cup formation, but plays a key role in driving macrophage lamellipodial spreading.","lang":"eng"}],"external_id":{"isi":["000400478300035"]},"publication_status":"published","page":"7258 - 7273","article_processing_charge":"No","title":"Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion","year":"2017","date_created":"2018-12-11T11:47:49Z","publication":"Journal of Biological Chemistry","issue":"17","doi":"10.1074/jbc.M116.766923","publication_identifier":{"issn":["0021-9258"]},"quality_controlled":"1","file":[{"file_id":"6971","file_size":5647880,"date_updated":"2020-07-14T12:47:37Z","checksum":"d488162874326a4bb056065fa549dc4a","relation":"main_file","access_level":"open_access","file_name":"2017_JBC_Horsthemke.pdf","content_type":"application/pdf","date_created":"2019-10-24T15:25:42Z","creator":"dernst"}],"ddc":["570"],"has_accepted_license":"1"},{"status":"public","day":"01","citation":{"chicago":"Synek, Lukáš, Nemanja Vukašinović, Ivan Kulich, Michal Hála, Klára Aldorfová, Matyas Fendrych, and Viktor Žárský. “EXO70C2 Is a Key Regulatory Factor for Optimal Tip Growth of Pollen.” Plant Physiology. American Society of Plant Biologists, 2017. https://doi.org/10.1104/pp.16.01282.","apa":"Synek, L., Vukašinović, N., Kulich, I., Hála, M., Aldorfová, K., Fendrych, M., & Žárský, V. (2017). EXO70C2 is a key regulatory factor for optimal tip growth of pollen. Plant Physiology. American Society of Plant Biologists. https://doi.org/10.1104/pp.16.01282","mla":"Synek, Lukáš, et al. “EXO70C2 Is a Key Regulatory Factor for Optimal Tip Growth of Pollen.” Plant Physiology, vol. 174, no. 1, American Society of Plant Biologists, 2017, pp. 223–40, doi:10.1104/pp.16.01282.","short":"L. Synek, N. Vukašinović, I. Kulich, M. Hála, K. Aldorfová, M. Fendrych, V. Žárský, Plant Physiology 174 (2017) 223–240.","ieee":"L. Synek et al., “EXO70C2 is a key regulatory factor for optimal tip growth of pollen,” Plant Physiology, vol. 174, no. 1. American Society of Plant Biologists, pp. 223–240, 2017.","ama":"Synek L, Vukašinović N, Kulich I, et al. EXO70C2 is a key regulatory factor for optimal tip growth of pollen. Plant Physiology. 2017;174(1):223-240. doi:10.1104/pp.16.01282","ista":"Synek L, Vukašinović N, Kulich I, Hála M, Aldorfová K, Fendrych M, Žárský V. 2017. EXO70C2 is a key regulatory factor for optimal tip growth of pollen. Plant Physiology. 174(1), 223–240."},"scopus_import":"1","_id":"669","publist_id":"7058","type":"journal_article","intvolume":" 174","oa_version":"Submitted Version","article_type":"original","date_updated":"2025-09-11T07:02:41Z","department":[{"_id":"JiFr"}],"publisher":"American Society of Plant Biologists","date_published":"2017-05-01T00:00:00Z","month":"05","language":[{"iso":"eng"}],"file_date_updated":"2020-07-14T12:47:37Z","oa":1,"isi":1,"publication":"Plant Physiology","publication_identifier":{"issn":["0032-0889"]},"doi":"10.1104/pp.16.01282","issue":"1","date_created":"2018-12-11T11:47:49Z","year":"2017","ddc":["580"],"has_accepted_license":"1","quality_controlled":"1","file":[{"content_type":"application/pdf","file_name":"2017_PlantPhysio_Synek.pdf","date_created":"2019-11-18T16:16:18Z","creator":"dernst","file_id":"7041","file_size":2176903,"date_updated":"2020-07-14T12:47:37Z","access_level":"open_access","checksum":"97155acc6aa5f0d0a78e0589a932fe02","relation":"main_file"}],"pmid":1,"volume":174,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"full_name":"Synek, Lukáš","first_name":"Lukáš","last_name":"Synek"},{"first_name":"Nemanja","last_name":"Vukašinović","full_name":"Vukašinović, Nemanja"},{"first_name":"Ivan","last_name":"Kulich","full_name":"Kulich, Ivan"},{"full_name":"Hála, Michal","last_name":"Hála","first_name":"Michal"},{"full_name":"Aldorfová, Klára","first_name":"Klára","last_name":"Aldorfová"},{"full_name":"Fendrych, Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9767-8699","last_name":"Fendrych","first_name":"Matyas"},{"first_name":"Viktor","last_name":"Žárský","full_name":"Žárský, Viktor"}],"title":"EXO70C2 is a key regulatory factor for optimal tip growth of pollen","external_id":{"pmid":["28356503"],"isi":["000402057200017"]},"publication_status":"published","abstract":[{"text":"The exocyst, a eukaryotic tethering complex, coregulates targeted exocytosis as an effector of small GTPases in polarized cell growth. In land plants, several exocyst subunits are encoded by double or triple paralogs, culminating in tens of EXO70 paralogs. Out of 23 Arabidopsis thaliana EXO70 isoforms, we analyzed seven isoforms expressed in pollen. Genetic and microscopic analyses of single mutants in EXO70A2, EXO70C1, EXO70C2, EXO70F1, EXO70H3, EXO70H5, and EXO70H6 genes revealed that only a loss-of-function EXO70C2 allele resulted in a significant male-specific transmission defect (segregation 40%:51%:9%) due to aberrant pollen tube growth. Mutant pollen tubes grown in vitro exhibited an enhanced growth rate and a decreased thickness of the tip cell wall, causing tip bursts. However, exo70C2 pollen tubes could frequently recover and restart their speedy elongation, resulting in a repetitive stop-and-go growth dynamics. A pollenspecific depletion of the closest paralog, EXO70C1, using artificial microRNA in the exo70C2 mutant background, resulted in a complete pollen-specific transmission defect, suggesting redundant functions of EXO70C1 and EXO70C2. Both EXO70C1 and EXO70C2, GFP tagged and expressed under the control of their native promoters, localized in the cytoplasm of pollen grains, pollen tubes, and also root trichoblast cells. The expression of EXO70C2-GFP complemented the aberrant growth of exo70C2 pollen tubes. The absent EXO70C2 interactions with core exocyst subunits in the yeast two-hybrid assay, cytoplasmic localization, and genetic effect suggest an unconventional EXO70 function possibly as a regulator of exocytosis outside the exocyst complex. In conclusion, EXO70C2 is a novel factor contributing to the regulation of optimal tip growth of Arabidopsis pollen tubes. ","lang":"eng"}],"article_processing_charge":"No","page":"223 - 240"},{"article_processing_charge":"No","page":"95 - 106","publication_status":"published","external_id":{"isi":["000404474000011"]},"abstract":[{"text":"We propose an efficient method to model paper tearing in the context of interactive modeling. The method uses geometrical information to automatically detect potential starting points of tears. We further introduce a new hybrid geometrical and physical-based method to compute the trajectory of tears while procedurally synthesizing high resolution details of the tearing path using a texture based approach. The results obtained are compared with real paper and with previous studies on the expected geometric paths of paper that tears.","lang":"eng"}],"title":"Interactive paper tearing","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"id":"2B14B676-F248-11E8-B48F-1D18A9856A87","full_name":"Schreck, Camille","last_name":"Schreck","first_name":"Camille"},{"full_name":"Rohmer, Damien","last_name":"Rohmer","first_name":"Damien"},{"last_name":"Hahmann","first_name":"Stefanie","full_name":"Hahmann, Stefanie"}],"volume":36,"quality_controlled":"1","main_file_link":[{"url":"https://hal.inria.fr/hal-01647113/file/eg_2017_schreck_paper_tearing.pdf","open_access":"1"}],"ddc":["000"],"date_created":"2018-12-11T11:47:49Z","year":"2017","publication_identifier":{"issn":["01677055"]},"doi":"10.1111/cgf.13110","issue":"2","publication":"Computer Graphics Forum","isi":1,"oa":1,"date_published":"2017-05-01T00:00:00Z","month":"05","language":[{"iso":"eng"}],"date_updated":"2025-09-11T07:02:03Z","department":[{"_id":"ChWo"}],"publisher":"Wiley","intvolume":" 36","oa_version":"Published Version","article_type":"original","type":"journal_article","publist_id":"7056","scopus_import":"1","_id":"670","citation":{"ista":"Schreck C, Rohmer D, Hahmann S. 2017. Interactive paper tearing. Computer Graphics Forum. 36(2), 95–106.","ama":"Schreck C, Rohmer D, Hahmann S. Interactive paper tearing. Computer Graphics Forum. 2017;36(2):95-106. doi:10.1111/cgf.13110","short":"C. Schreck, D. Rohmer, S. Hahmann, Computer Graphics Forum 36 (2017) 95–106.","ieee":"C. Schreck, D. Rohmer, and S. Hahmann, “Interactive paper tearing,” Computer Graphics Forum, vol. 36, no. 2. Wiley, pp. 95–106, 2017.","mla":"Schreck, Camille, et al. “Interactive Paper Tearing.” Computer Graphics Forum, vol. 36, no. 2, Wiley, 2017, pp. 95–106, doi:10.1111/cgf.13110.","apa":"Schreck, C., Rohmer, D., & Hahmann, S. (2017). Interactive paper tearing. Computer Graphics Forum. Wiley. https://doi.org/10.1111/cgf.13110","chicago":"Schreck, Camille, Damien Rohmer, and Stefanie Hahmann. “Interactive Paper Tearing.” Computer Graphics Forum. Wiley, 2017. https://doi.org/10.1111/cgf.13110."},"project":[{"name":"Deep Pictures: Creating Visual and Haptic Vector Images","call_identifier":"FWF","grant_number":"P 24352-N23","_id":"25357BD2-B435-11E9-9278-68D0E5697425"}],"status":"public","day":"01"},{"citation":{"mla":"Hilbe, Christian, et al. “Memory-n Strategies of Direct Reciprocity.” PNAS, vol. 114, no. 18, National Academy of Sciences, 2017, pp. 4715–20, doi:10.1073/pnas.1621239114.","chicago":"Hilbe, Christian, Vaquero Martinez, Krishnendu Chatterjee, and Martin Nowak. “Memory-n Strategies of Direct Reciprocity.” PNAS. National Academy of Sciences, 2017. https://doi.org/10.1073/pnas.1621239114.","apa":"Hilbe, C., Martinez, V., Chatterjee, K., & Nowak, M. (2017). Memory-n strategies of direct reciprocity. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1621239114","ama":"Hilbe C, Martinez V, Chatterjee K, Nowak M. Memory-n strategies of direct reciprocity. PNAS. 2017;114(18):4715-4720. doi:10.1073/pnas.1621239114","ista":"Hilbe C, Martinez V, Chatterjee K, Nowak M. 2017. Memory-n strategies of direct reciprocity. PNAS. 114(18), 4715–4720.","short":"C. Hilbe, V. Martinez, K. Chatterjee, M. Nowak, PNAS 114 (2017) 4715–4720.","ieee":"C. Hilbe, V. Martinez, K. Chatterjee, and M. Nowak, “Memory-n strategies of direct reciprocity,” PNAS, vol. 114, no. 18. National Academy of Sciences, pp. 4715–4720, 2017."},"project":[{"name":"Quantitative Graph Games: Theory and Applications","call_identifier":"FP7","grant_number":"279307","_id":"2581B60A-B435-11E9-9278-68D0E5697425"},{"_id":"2584A770-B435-11E9-9278-68D0E5697425","grant_number":"P 23499-N23","call_identifier":"FWF","name":"Modern Graph Algorithmic Techniques in Formal Verification"},{"grant_number":"S11407","_id":"25863FF4-B435-11E9-9278-68D0E5697425","name":"Game Theory","call_identifier":"FWF"}],"ec_funded":1,"day":"02","status":"public","isi":1,"oa":1,"date_published":"2017-05-02T00:00:00Z","language":[{"iso":"eng"}],"month":"05","date_updated":"2026-06-18T19:11:14Z","department":[{"_id":"KrCh"}],"publisher":"National Academy of Sciences","oa_version":"Published Version","intvolume":" 114","type":"journal_article","publist_id":"7053","scopus_import":"1","_id":"671","corr_author":"1","quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422766/"}],"ddc":["000"],"date_created":"2018-12-11T11:47:50Z","year":"2017","publication_identifier":{"issn":["0027-8424"]},"doi":"10.1073/pnas.1621239114","issue":"18","publication":"PNAS","article_processing_charge":"Yes (in subscription journal)","page":"4715 - 4720","external_id":{"isi":["000400358000050"],"pmid":["28420786"]},"publication_status":"published","abstract":[{"text":"Humans routinely use conditionally cooperative strategies when interacting in repeated social dilemmas. They are more likely to cooperate if others cooperated before, and are ready to retaliate if others defected. To capture the emergence of reciprocity, most previous models consider subjects who can only choose from a restricted set of representative strategies, or who react to the outcome of the very last round only. As players memorize more rounds, the dimension of the strategy space increases exponentially. This increasing computational complexity renders simulations for individuals with higher cognitive abilities infeasible, especially if multiplayer interactions are taken into account. Here, we take an axiomatic approach instead. We propose several properties that a robust cooperative strategy for a repeated multiplayer dilemma should have. These properties naturally lead to a unique class of cooperative strategies, which contains the classical Win-Stay Lose-Shift rule as a special case. A comprehensive numerical analysis for the prisoner's dilemma and for the public goods game suggests that strategies of this class readily evolve across various memory-n spaces. Our results reveal that successful strategies depend not only on how cooperative others were in the past but also on the respective context of cooperation.","lang":"eng"}],"title":"Memory-n strategies of direct reciprocity","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Christian","last_name":"Hilbe","orcid":"0000-0001-5116-955X","id":"2FDF8F3C-F248-11E8-B48F-1D18A9856A87","full_name":"Hilbe, Christian"},{"first_name":"Vaquero","last_name":"Martinez","full_name":"Martinez, Vaquero"},{"last_name":"Chatterjee","first_name":"Krishnendu","id":"2E5DCA20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4561-241X","full_name":"Chatterjee, Krishnendu"},{"full_name":"Nowak, Martin","last_name":"Nowak","first_name":"Martin"}],"volume":114,"pmid":1},{"title":"Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia","page":"902 - 909","article_processing_charge":"Yes","abstract":[{"lang":"eng","text":"Trafficking cells frequently transmigrate through epithelial and endothelial monolayers. How monolayers cooperate with the penetrating cells to support their transit is poorly understood. We studied dendritic cell (DC) entry into lymphatic capillaries as a model system for transendothelial migration. We find that the chemokine CCL21, which is the decisive guidance cue for intravasation, mainly localizes in the trans-Golgi network and intracellular vesicles of lymphatic endothelial cells. Upon DC transmigration, these Golgi deposits disperse and CCL21 becomes extracellularly enriched at the sites of endothelial cell-cell junctions. When we reconstitute the transmigration process in vitro, we find that secretion of CCL21-positive vesicles is triggered by a DC contact-induced calcium signal, and selective calcium chelation in lymphatic endothelium attenuates transmigration. Altogether, our data demonstrate a chemokine-mediated feedback between DCs and lymphatic endothelium, which facilitates transendothelial migration."}],"publication_status":"published","external_id":{"isi":["000402124100002"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"first_name":"Kari","last_name":"Vaahtomeri","orcid":"0000-0001-7829-3518","id":"368EE576-F248-11E8-B48F-1D18A9856A87","full_name":"Vaahtomeri, Kari"},{"full_name":"Brown, Markus","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","last_name":"Brown","first_name":"Markus"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert"},{"last_name":"De Vries","first_name":"Ingrid","full_name":"De Vries, Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-1073-744X","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","full_name":"Leithner, Alexander F","first_name":"Alexander F","last_name":"Leithner"},{"last_name":"Mehling","first_name":"Matthias","id":"3C23B994-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8599-1226","full_name":"Mehling, Matthias"},{"last_name":"Kaufmann","first_name":"Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","full_name":"Kaufmann, Walter"},{"last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"}],"volume":19,"has_accepted_license":"1","ddc":["570"],"corr_author":"1","file":[{"file_size":2248814,"date_updated":"2020-07-14T12:47:38Z","access_level":"open_access","relation":"main_file","checksum":"8fdddaab1f1d76a6ec9ca94dcb6b07a2","file_id":"5109","creator":"system","file_name":"IST-2017-900-v1+1_1-s2.0-S2211124717305211-main.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:14:54Z"}],"quality_controlled":"1","issue":"5","doi":"10.1016/j.celrep.2017.04.027","publication_identifier":{"issn":["2211-1247"]},"publication":"Cell Reports","year":"2017","date_created":"2018-12-11T11:47:50Z","month":"05","language":[{"iso":"eng"}],"date_published":"2017-05-02T00:00:00Z","publisher":"Cell Press","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"EM-Fac"}],"date_updated":"2025-09-10T14:27:34Z","isi":1,"oa":1,"file_date_updated":"2020-07-14T12:47:38Z","publist_id":"7052","_id":"672","scopus_import":"1","oa_version":"Published Version","intvolume":" 19","type":"journal_article","citation":{"chicago":"Vaahtomeri, Kari, Markus Brown, Robert Hauschild, Ingrid de Vries, Alexander F Leithner, Matthias Mehling, Walter Kaufmann, and Michael K Sixt. “Locally Triggered Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic Endothelia.” Cell Reports. Cell Press, 2017. https://doi.org/10.1016/j.celrep.2017.04.027.","apa":"Vaahtomeri, K., Brown, M., Hauschild, R., de Vries, I., Leithner, A. F., Mehling, M., … Sixt, M. K. (2017). Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2017.04.027","mla":"Vaahtomeri, Kari, et al. “Locally Triggered Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic Endothelia.” Cell Reports, vol. 19, no. 5, Cell Press, 2017, pp. 902–09, doi:10.1016/j.celrep.2017.04.027.","ieee":"K. Vaahtomeri et al., “Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia,” Cell Reports, vol. 19, no. 5. Cell Press, pp. 902–909, 2017.","short":"K. Vaahtomeri, M. Brown, R. Hauschild, I. de Vries, A.F. Leithner, M. Mehling, W. Kaufmann, M.K. Sixt, Cell Reports 19 (2017) 902–909.","ista":"Vaahtomeri K, Brown M, Hauschild R, de Vries I, Leithner AF, Mehling M, Kaufmann W, Sixt MK. 2017. Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia. Cell Reports. 19(5), 902–909.","ama":"Vaahtomeri K, Brown M, Hauschild R, et al. Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia. Cell Reports. 2017;19(5):902-909. doi:10.1016/j.celrep.2017.04.027"},"project":[{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FP7","grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","grant_number":"Y 564-B12"}],"pubrep_id":"900","day":"02","status":"public","ec_funded":1,"tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"}},{"OA_type":"closed access","day":"10","status":"public","citation":{"ama":"Altmeyer S, Lueptow R. Wave propagation reversal for wavy vortices in wide gap counter rotating cylindrical Couette flow. Physical Review E. 2017;95(5). doi:10.1103/PhysRevE.95.053103","ista":"Altmeyer S, Lueptow R. 2017. Wave propagation reversal for wavy vortices in wide gap counter rotating cylindrical Couette flow. Physical Review E. 95(5), 053103.","ieee":"S. Altmeyer and R. Lueptow, “Wave propagation reversal for wavy vortices in wide gap counter rotating cylindrical Couette flow,” Physical Review E, vol. 95, no. 5. American Physical Society, 2017.","short":"S. Altmeyer, R. Lueptow, Physical Review E 95 (2017).","mla":"Altmeyer, Sebastian, and Richard Lueptow. “Wave Propagation Reversal for Wavy Vortices in Wide Gap Counter Rotating Cylindrical Couette Flow.” Physical Review E, vol. 95, no. 5, 053103, American Physical Society, 2017, doi:10.1103/PhysRevE.95.053103.","apa":"Altmeyer, S., & Lueptow, R. (2017). Wave propagation reversal for wavy vortices in wide gap counter rotating cylindrical Couette flow. Physical Review E. American Physical Society. https://doi.org/10.1103/PhysRevE.95.053103","chicago":"Altmeyer, Sebastian, and Richard Lueptow. “Wave Propagation Reversal for Wavy Vortices in Wide Gap Counter Rotating Cylindrical Couette Flow.” Physical Review E. American Physical Society, 2017. https://doi.org/10.1103/PhysRevE.95.053103."},"intvolume":" 95","oa_version":"None","article_type":"original","type":"journal_article","publist_id":"7049","scopus_import":"1","_id":"673","isi":1,"date_published":"2017-05-10T00:00:00Z","language":[{"iso":"eng"}],"month":"05","date_updated":"2025-09-10T14:26:12Z","department":[{"_id":"BjHo"}],"publisher":"American Physical Society","date_created":"2018-12-11T11:47:50Z","year":"2017","article_number":"053103","doi":"10.1103/PhysRevE.95.053103","publication_identifier":{"issn":["2470-0045"]},"issue":"5","publication":"Physical Review E","corr_author":"1","quality_controlled":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"first_name":"Sebastian","last_name":"Altmeyer","full_name":"Altmeyer, Sebastian","orcid":"0000-0001-5964-0203","id":"2EE67FDC-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Lueptow","first_name":"Richard","full_name":"Lueptow, Richard"}],"volume":95,"pmid":1,"article_processing_charge":"No","external_id":{"isi":["000401233900007"],"pmid":["28618504"]},"publication_status":"published","abstract":[{"text":"We present a numerical study of wavy supercritical cylindrical Couette flow between counter-rotating cylinders in which the wavy pattern propagates either prograde with the inner cylinder or retrograde opposite the rotation of the inner cylinder. The wave propagation reversals from prograde to retrograde and vice versa occur at distinct values of the inner cylinder Reynolds number when the associated frequency of the wavy instability vanishes. The reversal occurs for both twofold and threefold symmetric wavy vortices. Moreover, the wave propagation reversal only occurs for sufficiently strong counter-rotation. The flow pattern reversal appears to be intrinsic in the system as either periodic boundary conditions or fixed end wall boundary conditions for different system sizes always result in the wave propagation reversal. We present a detailed bifurcation sequence and parameter space diagram with respect to retrograde behavior of wavy flows. The retrograde propagation of the instability occurs when the inner Reynolds number is about two times the outer Reynolds number. The mechanism for the retrograde propagation is associated with the inviscidly unstable region near the inner cylinder and the direction of the global average azimuthal velocity. Flow dynamics, spatio-temporal behavior, global mean angular velocity, and torque of the flow with the wavy pattern are explored.","lang":"eng"}],"title":"Wave propagation reversal for wavy vortices in wide gap counter rotating cylindrical Couette flow"},{"oa_version":"None","intvolume":" 27","type":"journal_article","publist_id":"7050","scopus_import":"1","_id":"674","isi":1,"date_published":"2017-05-09T00:00:00Z","month":"05","language":[{"iso":"eng"}],"date_updated":"2025-09-10T14:26:47Z","publisher":"Cell Press","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"ec_funded":1,"day":"09","status":"public","citation":{"ieee":"J. Schwarz et al., “Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6,” Current Biology, vol. 27, no. 9. Cell Press, pp. 1314–1325, 2017.","short":"J. Schwarz, V. Bierbaum, K. Vaahtomeri, R. Hauschild, M. Brown, I. de Vries, A.F. Leithner, A. Reversat, J. Merrin, T. Tarrant, M.T. Bollenbach, M.K. Sixt, Current Biology 27 (2017) 1314–1325.","ista":"Schwarz J, Bierbaum V, Vaahtomeri K, Hauschild R, Brown M, de Vries I, Leithner AF, Reversat A, Merrin J, Tarrant T, Bollenbach MT, Sixt MK. 2017. Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. Current Biology. 27(9), 1314–1325.","ama":"Schwarz J, Bierbaum V, Vaahtomeri K, et al. Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. Current Biology. 2017;27(9):1314-1325. doi:10.1016/j.cub.2017.04.004","chicago":"Schwarz, Jan, Veronika Bierbaum, Kari Vaahtomeri, Robert Hauschild, Markus Brown, Ingrid de Vries, Alexander F Leithner, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” Current Biology. Cell Press, 2017. https://doi.org/10.1016/j.cub.2017.04.004.","apa":"Schwarz, J., Bierbaum, V., Vaahtomeri, K., Hauschild, R., Brown, M., de Vries, I., … Sixt, M. K. (2017). Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2017.04.004","mla":"Schwarz, Jan, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” Current Biology, vol. 27, no. 9, Cell Press, 2017, pp. 1314–25, doi:10.1016/j.cub.2017.04.004."},"project":[{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"},{"grant_number":"Y 564-B12","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FWF"}],"author":[{"id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","full_name":"Schwarz, Jan","first_name":"Jan","last_name":"Schwarz"},{"first_name":"Veronika","last_name":"Bierbaum","id":"3FD04378-F248-11E8-B48F-1D18A9856A87","full_name":"Bierbaum, Veronika"},{"last_name":"Vaahtomeri","first_name":"Kari","full_name":"Vaahtomeri, Kari","orcid":"0000-0001-7829-3518","id":"368EE576-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","full_name":"Brown, Markus","first_name":"Markus","last_name":"Brown"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid","first_name":"Ingrid","last_name":"De Vries"},{"last_name":"Leithner","first_name":"Alexander F","orcid":"0000-0002-1073-744X","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","full_name":"Leithner, Alexander F"},{"full_name":"Reversat, Anne","orcid":"0000-0003-0666-8928","id":"35B76592-F248-11E8-B48F-1D18A9856A87","first_name":"Anne","last_name":"Reversat"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack"},{"last_name":"Tarrant","first_name":"Teresa","full_name":"Tarrant, Teresa"},{"first_name":"Tobias","last_name":"Bollenbach","full_name":"Bollenbach, Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4398-476X"},{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","volume":27,"article_processing_charge":"No","page":"1314 - 1325","external_id":{"isi":["000400741700021"]},"publication_status":"published","abstract":[{"lang":"eng","text":"Navigation of cells along gradients of guidance cues is a determining step in many developmental and immunological processes. Gradients can either be soluble or immobilized to tissues as demonstrated for the haptotactic migration of dendritic cells (DCs) toward higher concentrations of immobilized chemokine CCL21. To elucidate how gradient characteristics govern cellular response patterns, we here introduce an in vitro system allowing to track migratory responses of DCs to precisely controlled immobilized gradients of CCL21. We find that haptotactic sensing depends on the absolute CCL21 concentration and local steepness of the gradient, consistent with a scenario where DC directionality is governed by the signal-to-noise ratio of CCL21 binding to the receptor CCR7. We find that the conditions for optimal DC guidance are perfectly provided by the CCL21 gradients we measure in vivo. Furthermore, we find that CCR7 signal termination by the G-protein-coupled receptor kinase 6 (GRK6) is crucial for haptotactic but dispensable for chemotactic CCL21 gradient sensing in vitro and confirm those observations in vivo. These findings suggest that stable, tissue-bound CCL21 gradients as sustainable “roads” ensure optimal guidance in vivo."}],"title":"Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6","date_created":"2018-12-11T11:47:51Z","year":"2017","publication_identifier":{"issn":["09609822"]},"doi":"10.1016/j.cub.2017.04.004","issue":"9","publication":"Current Biology","corr_author":"1","quality_controlled":"1"}]