---
OA_place: publisher
_id: '17336'
abstract:
- lang: eng
text: "This thesis deals with the study of stochastic processes and their ergodicity
properties. The\r\nvariety of problems encountered calls for a set of different
approaches, ranging from classical to\r\nmodern ones: a special place is held
by probabilistic methods based on couplings, by functional\r\ninequalities, and
by the theory of gradient flows in the space of measures.\r\n\r\nThe material
is organized as follows. Chapter 1 contains the introduction to this thesis, starting\r\nwith
a general presentation of some of the relevant topics. Section 1.1 is dedicated
to the\r\ntheory of gradient flows in metric spaces, and introduces the first
contribution of this thesis\r\n[DSMP24], which is presented in detail in Chapter
2. Section 1.2 moves to the topic of\r\ncurvature of Markov chains, concluding
with a brief description of our second contribution\r\n[Ped23], which is included
in Chapter 3. Section 1.3 discusses applications of stochastic\r\nprocesses to
the theory of sampling, in particular the recent framework of score-based diffusion\r\nmodels,
and our contribution [PMM24], which is contained in Chapter 4. Section 1.4 discusses\r\nsome
related problems, concerning the regularization properties of the heat flow. It
serves\r\nas a motivation for the work [BP24], which we report in Chapter 5. Finally,
Section 1.5\r\ndiscusses the last contribution of this thesis, which can be found
in Chapter 6. It deals with\r\nthe convergence to equilibrium of a particular
stochastic model from quantitative genetics:\r\nthis is established via some functional
inequalities, which we prove with probabilistic arguments\r\nbased on couplings.\r\n"
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Francesco
full_name: Pedrotti, Francesco
id: d3ac8ac6-dc8d-11ea-abe3-e2a9628c4c3c
last_name: Pedrotti
citation:
ama: Pedrotti F. Functional inequalities and convergence of stochastic processes.
2024. doi:10.15479/at:ista:17336
apa: Pedrotti, F. (2024). Functional inequalities and convergence of stochastic
processes. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:17336
chicago: Pedrotti, Francesco. “Functional Inequalities and Convergence of Stochastic
Processes.” Institute of Science and Technology Austria, 2024. https://doi.org/10.15479/at:ista:17336.
ieee: F. Pedrotti, “Functional inequalities and convergence of stochastic processes,”
Institute of Science and Technology Austria, 2024.
ista: Pedrotti F. 2024. Functional inequalities and convergence of stochastic processes.
Institute of Science and Technology Austria.
mla: Pedrotti, Francesco. Functional Inequalities and Convergence of Stochastic
Processes. Institute of Science and Technology Austria, 2024, doi:10.15479/at:ista:17336.
short: F. Pedrotti, Functional Inequalities and Convergence of Stochastic Processes,
Institute of Science and Technology Austria, 2024.
corr_author: '1'
das_tickbox: '0'
date_created: 2024-07-29T09:14:14Z
date_published: 2024-07-31T00:00:00Z
date_updated: 2026-10-02T12:22:41Z
day: '31'
ddc:
- '500'
- '510'
- '515'
- '519'
degree_awarded: PhD
department:
- _id: GradSch
- _id: JaMa
doi: 10.15479/at:ista:17336
doi_confirm: '1'
ec_funded: 1
file:
- access_level: open_access
checksum: 11650bab714ef85ad43a287060850523
content_type: application/pdf
creator: fpedrott
date_created: 2024-08-02T09:23:26Z
date_updated: 2024-08-02T09:23:26Z
file_id: '17366'
file_name: thesis_final.pdf
file_size: 2941599
relation: main_file
success: 1
- access_level: closed
checksum: c30ba5611941226cf1bfc867c25b1e80
content_type: application/x-zip-compressed
creator: fpedrott
date_created: 2024-08-02T09:27:15Z
date_updated: 2024-08-02T09:27:15Z
file_id: '17367'
file_name: thesis_final_source.zip
file_size: 6293375
relation: source_file
file_date_updated: 2024-08-02T09:27:15Z
fulldoi: https://doi.org/10.15479/at:ista:17336
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '07'
oa: 1
oa_version: Published Version
page: '183'
project:
- _id: 256E75B8-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '716117'
name: Optimal Transport and Stochastic Dynamics
- _id: fc31cba2-9c52-11eb-aca3-ff467d239cd2
grant_number: F6504
name: Taming Complexity in Partial Differential Systems
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '17351'
relation: part_of_dissertation
status: public
- id: '17143'
relation: part_of_dissertation
status: public
- id: '17352'
relation: part_of_dissertation
status: public
- id: '17353'
relation: part_of_dissertation
status: public
- id: '17350'
relation: part_of_dissertation
status: public
researchdata_availability: no
status: public
supervisor:
- first_name: Jan
full_name: Maas, Jan
id: 4C5696CE-F248-11E8-B48F-1D18A9856A87
last_name: Maas
orcid: 0000-0002-0845-1338
supplementarymaterial: no
title: Functional inequalities and convergence of stochastic processes
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: dissertation
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
year: '2024'
...
---
OA_place: publisher
OA_type: gold
_id: '18897'
abstract:
- lang: eng
text: 'Score-based generative models (SGMs) are powerful tools to sample from complex
data distributions. Their underlying idea is to (i) run a forward process for
time T1 by adding noise to the data, (ii) estimate its score function, and (iii)
use such estimate to run a reverse process. As the reverse process is initialized
with the stationary distribution of the forward one, the existing analysis paradigm
requires T1→∞. This is however problematic: from a theoretical viewpoint, for
a given precision of the score approximation, the convergence guarantee fails
as T1 diverges; from a practical viewpoint, a large T1 increases computational
costs and leads to error propagation. This paper addresses the issue by considering
a version of the popular predictor-corrector scheme: after running the forward
process, we first estimate the final distribution via an inexact Langevin dynamics
and then revert the process. Our key technical contribution is to provide convergence
guarantees which require to run the forward process only for a fixed finite time
T1. Our bounds exhibit a mild logarithmic dependence on the input dimension and
the subgaussian norm of the target distribution, have minimal assumptions on the
data, and require only to control the L2 loss on the score approximation, which
is the quantity minimized in practice.'
acknowledgement: "Francesco Pedrotti and Jan Maas acknowledge support by the Austrian
Science Fund (FWF) project 10.55776/F65. Marco Mondelli acknowledges support by
the 2019 Lopez-Loreta prize.\r\n"
alternative_title:
- TMLR
article_processing_charge: No
arxiv: 1
author:
- first_name: Francesco
full_name: Pedrotti, Francesco
id: d3ac8ac6-dc8d-11ea-abe3-e2a9628c4c3c
last_name: Pedrotti
- first_name: Jan
full_name: Maas, Jan
id: 4C5696CE-F248-11E8-B48F-1D18A9856A87
last_name: Maas
orcid: 0000-0002-0845-1338
- first_name: Marco
full_name: Mondelli, Marco
id: 27EB676C-8706-11E9-9510-7717E6697425
last_name: Mondelli
orcid: 0000-0002-3242-7020
citation:
ama: 'Pedrotti F, Maas J, Mondelli M. Improved convergence of score-based diffusion
models via prediction-correction. In: Transactions on Machine Learning Research.
; 2024.'
apa: Pedrotti, F., Maas, J., & Mondelli, M. (2024). Improved convergence of
score-based diffusion models via prediction-correction. In Transactions on
Machine Learning Research.
chicago: Pedrotti, Francesco, Jan Maas, and Marco Mondelli. “Improved Convergence
of Score-Based Diffusion Models via Prediction-Correction.” In Transactions
on Machine Learning Research, 2024.
ieee: F. Pedrotti, J. Maas, and M. Mondelli, “Improved convergence of score-based
diffusion models via prediction-correction,” in Transactions on Machine Learning
Research, 2024.
ista: Pedrotti F, Maas J, Mondelli M. 2024. Improved convergence of score-based
diffusion models via prediction-correction. Transactions on Machine Learning Research.
, TMLR, .
mla: Pedrotti, Francesco, et al. “Improved Convergence of Score-Based Diffusion
Models via Prediction-Correction.” Transactions on Machine Learning Research,
2024.
short: F. Pedrotti, J. Maas, M. Mondelli, in:, Transactions on Machine Learning
Research, 2024.
corr_author: '1'
das_tickbox: '0'
date_created: 2025-01-27T12:18:05Z
date_published: 2024-06-01T00:00:00Z
date_updated: 2026-10-02T12:22:41Z
day: '01'
ddc:
- '000'
department:
- _id: JaMa
- _id: MaMo
external_id:
arxiv:
- '2305.14164'
file:
- access_level: open_access
checksum: 76a1fd5afd8ee6f7ae0e5912d7dbf6b4
content_type: application/pdf
creator: dernst
date_created: 2025-01-27T12:19:44Z
date_updated: 2025-01-27T12:19:44Z
file_id: '18898'
file_name: 2024_TMLR_Pedrotti.pdf
file_size: 780315
relation: main_file
success: 1
file_date_updated: 2025-01-27T12:19:44Z
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '06'
oa: 1
oa_version: Published Version
project:
- _id: fc31cba2-9c52-11eb-aca3-ff467d239cd2
grant_number: F6504
name: Taming Complexity in Partial Differential Systems
- _id: 059876FA-7A3F-11EA-A408-12923DDC885E
name: Prix Lopez-Loretta 2019 - Marco Mondelli
publication: Transactions on Machine Learning Research
publication_identifier:
issn:
- 2835-8856
publication_status: published
quality_controlled: '1'
related_material:
record:
- id: '17350'
relation: earlier_version
status: public
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: no
title: Improved convergence of score-based diffusion models via prediction-correction
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: conference
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
year: '2024'
...
---
OA_place: repository
_id: '17350'
abstract:
- lang: eng
text: "Score-based generative models (SGMs) are powerful tools to sample from\r\ncomplex
data distributions. Their underlying idea is to (i) run a forward\r\nprocess for
time $T_1$ by adding noise to the data, (ii) estimate its score\r\nfunction, and
(iii) use such estimate to run a reverse process. As the reverse\r\nprocess is
initialized with the stationary distribution of the forward one, the\r\nexisting
analysis paradigm requires $T_1\\to\\infty$. This is however\r\nproblematic: from
a theoretical viewpoint, for a given precision of the score\r\napproximation,
the convergence guarantee fails as $T_1$ diverges; from a\r\npractical viewpoint,
a large $T_1$ increases computational costs and leads to\r\nerror propagation.
This paper addresses the issue by considering a version of\r\nthe popular predictor-corrector
scheme: after running the forward process, we\r\nfirst estimate the final distribution
via an inexact Langevin dynamics and then\r\nrevert the process. Our key technical
contribution is to provide convergence\r\nguarantees which require to run the
forward process only for a fixed finite\r\ntime $T_1$. Our bounds exhibit a mild
logarithmic dependence on the input\r\ndimension and the subgaussian norm of the
target distribution, have minimal\r\nassumptions on the data, and require only
to control the $L^2$ loss on the\r\nscore approximation, which is the quantity
minimized in practice."
article_processing_charge: No
arxiv: 1
author:
- first_name: Francesco
full_name: Pedrotti, Francesco
id: d3ac8ac6-dc8d-11ea-abe3-e2a9628c4c3c
last_name: Pedrotti
- first_name: Jan
full_name: Maas, Jan
id: 4C5696CE-F248-11E8-B48F-1D18A9856A87
last_name: Maas
orcid: 0000-0002-0845-1338
- first_name: Marco
full_name: Mondelli, Marco
id: 27EB676C-8706-11E9-9510-7717E6697425
last_name: Mondelli
orcid: 0000-0002-3242-7020
citation:
ama: Pedrotti F, Maas J, Mondelli M. Improved convergence of score-based diffusion
models via prediction-correction. arXiv. doi:10.48550/arXiv.2305.14164
apa: Pedrotti, F., Maas, J., & Mondelli, M. (n.d.). Improved convergence of
score-based diffusion models via prediction-correction. arXiv. https://doi.org/10.48550/arXiv.2305.14164
chicago: Pedrotti, Francesco, Jan Maas, and Marco Mondelli. “Improved Convergence
of Score-Based Diffusion Models via Prediction-Correction.” ArXiv, n.d.
https://doi.org/10.48550/arXiv.2305.14164.
ieee: F. Pedrotti, J. Maas, and M. Mondelli, “Improved convergence of score-based
diffusion models via prediction-correction,” arXiv. .
ista: Pedrotti F, Maas J, Mondelli M. Improved convergence of score-based diffusion
models via prediction-correction. arXiv, 10.48550/arXiv.2305.14164.
mla: Pedrotti, Francesco, et al. “Improved Convergence of Score-Based Diffusion
Models via Prediction-Correction.” ArXiv, doi:10.48550/arXiv.2305.14164.
short: F. Pedrotti, J. Maas, M. Mondelli, ArXiv (n.d.).
corr_author: '1'
das_tickbox: '0'
date_created: 2024-07-31T07:56:40Z
date_published: 2024-06-06T00:00:00Z
date_updated: 2026-10-02T12:22:41Z
day: '06'
department:
- _id: JaMa
- _id: MaMo
doi: 10.48550/arXiv.2305.14164
external_id:
arxiv:
- '2305.14164'
fulldoi: https://doi.org/10.48550/arXiv.2305.14164
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.48550/arXiv.2305.14164
month: '06'
oa: 1
oa_version: Preprint
project:
- _id: fc31cba2-9c52-11eb-aca3-ff467d239cd2
grant_number: F6504
name: Taming Complexity in Partial Differential Systems
- _id: 059876FA-7A3F-11EA-A408-12923DDC885E
name: Prix Lopez-Loretta 2019 - Marco Mondelli
publication: arXiv
publication_status: draft
related_material:
record:
- id: '17336'
relation: dissertation_contains
status: public
- id: '18897'
relation: later_version
status: public
researchdata_availability: no
status: public
supplementarymaterial: no
title: Improved convergence of score-based diffusion models via prediction-correction
type: preprint
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
year: '2024'
...
---
OA_place: repository
OA_type: green
_id: '22776'
article_processing_charge: No
author:
- first_name: Yunlong
full_name: Du, Yunlong
last_name: Du
citation:
ama: Du Y. SeRrbJ609. 2024. doi:10.6084/m9.figshare.25448191
apa: Du, Y. (2024). SeRrbJ609. Repository. https://doi.org/10.6084/m9.figshare.25448191
chicago: Du, Yunlong. “SeRrbJ609.” Repository, 2024. https://doi.org/10.6084/m9.figshare.25448191.
ieee: Y. Du, “SeRrbJ609.” Repository, 2024.
ista: Du Y. 2024. SeRrbJ609, Repository, 10.6084/m9.figshare.25448191.
mla: Du, Yunlong. SeRrbJ609. Repository, 2024, doi:10.6084/m9.figshare.25448191.
short: Y. Du, (2024).
date_created: 2026-09-03T09:17:46Z
date_published: 2024-03-21T00:00:00Z
date_updated: 2026-10-02T12:31:36Z
day: '21'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.6084/m9.figshare.25448191
fulldoi: https://doi.org/10.6084/m9.figshare.25448191
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.25448191
month: '03'
oa: 1
oa_version: None
publisher: Repository
related_material:
record:
- id: '17103'
relation: research_data
status: public
status: public
title: SeRrbJ609
type: research_data_reference
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
year: '2024'
...
---
DOAJ_listed: '1'
_id: '17103'
abstract:
- lang: eng
text: Phytoalexin sakuranetin functions in resistance against rice blast. However,
the mechanisms underlying the effects of sakuranetin remains elusive. Here, we
report that rice lines expressing resistance (R) genes were found to contain high
levels of sakuranetin, which correlates with attenuated endocytic trafficking
of plasma membrane (PM) proteins. Exogenous and endogenous sakuranetin attenuates
the endocytosis of various PM proteins and the fungal effector PWL2. Moreover,
accumulation of the avirulence protein AvrCO39, resulting from uptake into rice
cells by Magnaporthe oryzae, was reduced following treatment with sakuranetin.
Pharmacological manipulation of clathrin-mediated endocytic (CME) suggests that
this pathway is targeted by sakuranetin. Indeed, attenuation of CME by sakuranetin
is sufficient to convey resistance against rice blast. Our data reveals a mechanism
of rice against M. oryzae by increasing sakuranetin levels and repressing the
CME of pathogen effectors, which is distinct from the action of many R genes that
mainly function by modulating transcription.
acknowledgement: We thank Professor Jianqiang Wu (Kunming Institute of Botany, Chinese
Academy of Sciences) for his generous support with the sakuranetin measurement.
We thank International Rice Research Institute (IRRI) for provision of the rice
NILs. We thank Professor Zhongkai Zhang (Yunnan Academy of Agricultural Sciences)
for his generous support with subcellular structure observation of rice roots. We
thank Professor Barbara Valent (Kansas State University) for her generously provision
of the plasmid containing the PWL2 gene. We thank Professor Zuhua He (Chinese Academy
of Sciences) for the gift of the transgenic rice line expressing the Pigm gene,
OsNPR1-RNAi mutant line and ROD1-overexpression rice line. We thank Professor Yinong
Yang (The Pennsylvania State University) for provision of the rice line NahG. We
thank Professor Muyuan Zhu (Zhejiang University) for provision of the transgenic
plants overexpressing miR393a. We thank Professor Zhengge Zhu (Hebei Normal University)
for provision of the rice line overexpressing OsPIN3t-GFP. We thank Professor Yanhua
Qi (Zhejiang University) for provision of the arf12 mutant line. Thanks also go
to Professor Jean-Benoit Morel (Plant Health Institute of Montpellier) for provision
of the fungus M. oryzae strain Guy11 (AvrCo39-mRFP). This work was supported by
grants from the National Natural Science Foundation of China (Grant Nos. 32260085,
31460453, 31660501, 31860064, 31760500 and 31901870), the Major Special Program
for Scientific Research, Education Department of Yunnan Province (Grant No. ZD2015005).
The project was also sponsored by SRF for ROCS, SEM (Grant No. [2013] 1792), the
Key Projects of Applied Basic Research Plan of Yunnan Province (Grant No. 2017FA018,
202301AS070082), the Major Science and Technology Project in Yunnan Province (202102AE090042,
202202AE090036 and 202102AE090017), the Young and Middle-Aged Academic and Technical
Leaders Reserve Talent Program in Yunnan Province (202205AC160076), the China Postdoctoral
Science Foundation (2019M653849XB) and the National Key Research and Development
Program of China (2023YFE0107500).
article_number: '3437'
article_processing_charge: Yes
article_type: original
author:
- first_name: Lihui
full_name: Jiang, Lihui
last_name: Jiang
- first_name: Xiaoyan
full_name: Zhang, Xiaoyan
last_name: Zhang
- first_name: Yiting
full_name: Zhao, Yiting
last_name: Zhao
- first_name: Haiyan
full_name: Zhu, Haiyan
last_name: Zhu
- first_name: Qijing
full_name: Fu, Qijing
last_name: Fu
- first_name: Xinqi
full_name: Lu, Xinqi
last_name: Lu
- first_name: Wuying
full_name: Huang, Wuying
last_name: Huang
- first_name: Xinyue
full_name: Yang, Xinyue
last_name: Yang
- first_name: Xuan
full_name: Zhou, Xuan
last_name: Zhou
- first_name: Lixia
full_name: Wu, Lixia
last_name: Wu
- first_name: Ao
full_name: Yang, Ao
last_name: Yang
- first_name: Xie
full_name: He, Xie
last_name: He
- first_name: Man
full_name: Dong, Man
last_name: Dong
- first_name: Ziai
full_name: Peng, Ziai
last_name: Peng
- first_name: Jing
full_name: Yang, Jing
last_name: Yang
- first_name: Liwei
full_name: Guo, Liwei
last_name: Guo
- first_name: Jiancheng
full_name: Wen, Jiancheng
last_name: Wen
- first_name: Huichuan
full_name: Huang, Huichuan
last_name: Huang
- first_name: Yong
full_name: Xie, Yong
last_name: Xie
- first_name: Shusheng
full_name: Zhu, Shusheng
last_name: Zhu
- first_name: Chengyun
full_name: Li, Chengyun
last_name: Li
- first_name: Xiahong
full_name: He, Xiahong
last_name: He
- first_name: Youyong
full_name: Zhu, Youyong
last_name: Zhu
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Yunlong
full_name: Du, Yunlong
last_name: Du
citation:
ama: Jiang L, Zhang X, Zhao Y, et al. Phytoalexin sakuranetin attenuates endocytosis
and enhances resistance to rice blast. Nature Communications. 2024;15.
doi:10.1038/s41467-024-47746-y
apa: Jiang, L., Zhang, X., Zhao, Y., Zhu, H., Fu, Q., Lu, X., … Du, Y. (2024). Phytoalexin
sakuranetin attenuates endocytosis and enhances resistance to rice blast. Nature
Communications. Springer Nature. https://doi.org/10.1038/s41467-024-47746-y
chicago: Jiang, Lihui, Xiaoyan Zhang, Yiting Zhao, Haiyan Zhu, Qijing Fu, Xinqi
Lu, Wuying Huang, et al. “Phytoalexin Sakuranetin Attenuates Endocytosis and Enhances
Resistance to Rice Blast.” Nature Communications. Springer Nature, 2024.
https://doi.org/10.1038/s41467-024-47746-y.
ieee: L. Jiang et al., “Phytoalexin sakuranetin attenuates endocytosis and
enhances resistance to rice blast,” Nature Communications, vol. 15. Springer
Nature, 2024.
ista: Jiang L, Zhang X, Zhao Y, Zhu H, Fu Q, Lu X, Huang W, Yang X, Zhou X, Wu L,
Yang A, He X, Dong M, Peng Z, Yang J, Guo L, Wen J, Huang H, Xie Y, Zhu S, Li
C, He X, Zhu Y, Friml J, Du Y. 2024. Phytoalexin sakuranetin attenuates endocytosis
and enhances resistance to rice blast. Nature Communications. 15, 3437.
mla: Jiang, Lihui, et al. “Phytoalexin Sakuranetin Attenuates Endocytosis and Enhances
Resistance to Rice Blast.” Nature Communications, vol. 15, 3437, Springer
Nature, 2024, doi:10.1038/s41467-024-47746-y.
short: L. Jiang, X. Zhang, Y. Zhao, H. Zhu, Q. Fu, X. Lu, W. Huang, X. Yang, X.
Zhou, L. Wu, A. Yang, X. He, M. Dong, Z. Peng, J. Yang, L. Guo, J. Wen, H. Huang,
Y. Xie, S. Zhu, C. Li, X. He, Y. Zhu, J. Friml, Y. Du, Nature Communications 15
(2024).
das_tickbox: '1'
dataavailabilitystatement: All data supporting the findings of this study are available
within the paper, its Supplementary Information file, and the Source Data file.
The LC-MS/MS data and microscopy data have been deposited at the public repository
figshare with the dataset identifier SeRrbJ609 [https://doi.org/10.6084/m9.figshare.25448191.v3].
Original data points in graphs and qPCR data are shown in the Source Data files.
Source data are provided with this paper.
date_created: 2024-06-03T08:54:50Z
date_published: 2024-04-23T00:00:00Z
date_updated: 2026-10-02T12:32:10Z
day: '23'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-024-47746-y
external_id:
isi:
- '001207290500013'
pmid:
- '38653755'
file:
- access_level: open_access
checksum: 80cb2f2c538e81064f4836b54bc313ca
content_type: application/pdf
creator: dernst
date_created: 2024-06-03T12:05:10Z
date_updated: 2024-06-03T12:05:10Z
file_id: '17110'
file_name: 2024_NatureComm_Jiang.pdf
file_size: 8013695
relation: main_file
success: 1
file_date_updated: 2024-06-03T12:05:10Z
fulldoi: https://doi.org/10.1038/s41467-024-47746-y
has_accepted_license: '1'
intvolume: ' 15'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '22776'
relation: used_in_publication
status: public
- id: '23039'
relation: research_data
status: public
researchdata_availability: yes (supplementary data)
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice
blast
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2024'
...
---
APC_amount: 2792,52 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '15033'
abstract:
- lang: eng
text: The GNOM (GN) Guanine nucleotide Exchange Factor for ARF small GTPases (ARF-GEF)
is among the best studied trafficking regulators in plants, playing crucial and
unique developmental roles in patterning and polarity. The current models place
GN at the Golgi apparatus (GA), where it mediates secretion/recycling, and at
the plasma membrane (PM) presumably contributing to clathrin-mediated endocytosis
(CME). The mechanistic basis of the developmental function of GN, distinct from
the other ARF-GEFs including its closest homologue GNOM-LIKE1 (GNL1), remains
elusive. Insights from this study largely extend the current notions of GN function.
We show that GN, but not GNL1, localizes to the cell periphery at long-lived structures
distinct from clathrin-coated pits, while CME and secretion proceed normally in
gn knockouts. The functional GN mutant variant GNfewerroots,
absent from the GA, suggests that the cell periphery is the major site of GN action
responsible for its developmental function. Following inhibition by Brefeldin
A, GN, but not GNL1, relocates to the PM likely on exocytic vesicles, suggesting
selective molecular associations en route to the cell periphery. A study of GN-GNL1
chimeric ARF-GEFs indicates that all GN domains contribute to the specific GN
function in a partially redundant manner. Together, this study offers significant
steps toward the elucidation of the mechanism underlying unique cellular and development
functions of GNOM.
acknowledgement: The authors would like to gratefully acknowledge Dr Xixi Zhang for
cloning the GNL1/pDONR221 construct and for useful discussions.H2020 European Research
Council Advanced Grant ETAP742985 to Jiří Friml, Austrian Science Fund I 3630-B25
to Jiří Friml
article_processing_charge: Yes
article_type: original
author:
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Ivana
full_name: Matijevic, Ivana
id: 83c17ce3-15b2-11ec-abd3-f486545870bd
last_name: Matijevic
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Adamowski M, Matijevic I, Friml J. Developmental patterning function of GNOM
ARF-GEF mediated from the cell periphery. eLife. 2024;13. doi:10.7554/elife.68993
apa: Adamowski, M., Matijevic, I., & Friml, J. (2024). Developmental patterning
function of GNOM ARF-GEF mediated from the cell periphery. ELife. eLife
Sciences Publications. https://doi.org/10.7554/elife.68993
chicago: Adamowski, Maciek, Ivana Matijevic, and Jiří Friml. “Developmental Patterning
Function of GNOM ARF-GEF Mediated from the Cell Periphery.” ELife. eLife
Sciences Publications, 2024. https://doi.org/10.7554/elife.68993.
ieee: M. Adamowski, I. Matijevic, and J. Friml, “Developmental patterning function
of GNOM ARF-GEF mediated from the cell periphery,” eLife, vol. 13. eLife
Sciences Publications, 2024.
ista: Adamowski M, Matijevic I, Friml J. 2024. Developmental patterning function
of GNOM ARF-GEF mediated from the cell periphery. eLife. 13.
mla: Adamowski, Maciek, et al. “Developmental Patterning Function of GNOM ARF-GEF
Mediated from the Cell Periphery.” ELife, vol. 13, eLife Sciences Publications,
2024, doi:10.7554/elife.68993.
short: M. Adamowski, I. Matijevic, J. Friml, ELife 13 (2024).
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: All data generated or analysed during this study are included
in the manuscript and supporting files. No additional source files were provided.
date_created: 2024-02-27T07:10:11Z
date_published: 2024-02-21T00:00:00Z
date_updated: 2026-10-15T08:07:21Z
day: '21'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.7554/elife.68993
ec_funded: 1
external_id:
isi:
- '001174278000001'
pmid:
- '38381485'
file:
- access_level: open_access
checksum: b2b2d583b433823af731842f1420113e
content_type: application/pdf
creator: dernst
date_created: 2024-07-22T11:51:50Z
date_updated: 2024-07-22T11:51:50Z
file_id: '17310'
file_name: 2024_eLife_Adamowski.pdf
file_size: 15675744
relation: main_file
success: 1
file_date_updated: 2024-07-22T11:51:50Z
fulldoi: https://doi.org/10.7554/elife.68993
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 3AC91DDA-15DF-11EA-824D-93A3E7B544D1
call_identifier: FWF
name: FWF Open Access Fund
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
researchdata_availability: yes (supplementary data)
scopus_import: '1'
status: public
supplementarymaterial: no
title: Developmental patterning function of GNOM ARF-GEF mediated from the cell periphery
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 13
year: '2024'
...
---
_id: '14826'
abstract:
- lang: eng
text: The plant-signaling molecule auxin triggers fast and slow cellular responses
across land plants and algae. The nuclear auxin pathway mediates gene expression
and controls growth and development in land plants, but this pathway is absent
from algal sister groups. Several components of rapid responses have been identified
in Arabidopsis, but it is unknown if these are part of a conserved mechanism.
We recently identified a fast, proteome-wide phosphorylation response to auxin.
Here, we show that this response occurs across 5 land plant and algal species
and converges on a core group of shared targets. We found conserved rapid physiological
responses to auxin in the same species and identified rapidly accelerated fibrosarcoma
(RAF)-like protein kinases as central mediators of auxin-triggered phosphorylation
across species. Genetic analysis connects this kinase to both auxin-triggered
protein phosphorylation and rapid cellular response, thus identifying an ancient
mechanism for fast auxin responses in the green lineage.
acknowledgement: 'We are grateful to Asuka Shitaku and Eri Koide for generating and
sharing the Marchantia PRAF-mCitrine line and Peng-Cheng Wang for sharing the Arabidopsis
raf mutant. We are grateful to our team members for discussions and helpful advice.
This work was supported by funding from the Netherlands Organization for Scientific
Research (NWO): VICI grant 865.14.001 and ENW-KLEIN OCENW.KLEIN.027 grants to D.W.;
VENI grant VI.VENI.212.003 to A.K.; the European Research Council AdG DIRNDL (contract
number 833867) to D.W.; CoG CATCH to J.S.; StG CELLONGATE (contract 803048) to M.F.;
and AdG ETAP (contract 742985) to J.F.; MEXT KAKENHI grant number JP19H05675 to
T.K.; JSPS KAKENHI grant number JP20H03275 to R.N.; Takeda Science Foundation to
R.N.; and the Austrian Science Fund (FWF, P29988) to J.F.'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Andre
full_name: Kuhn, Andre
last_name: Kuhn
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Sumanth
full_name: Mutte, Sumanth
last_name: Mutte
- first_name: Shiv Mani
full_name: Dubey, Shiv Mani
last_name: Dubey
- first_name: Vanessa Polet
full_name: Carrillo Carrasco, Vanessa Polet
last_name: Carrillo Carrasco
- first_name: Sjef
full_name: Boeren, Sjef
last_name: Boeren
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Jasper
full_name: Koehorst, Jasper
last_name: Koehorst
- first_name: Takayuki
full_name: Kohchi, Takayuki
last_name: Kohchi
- first_name: Ryuichi
full_name: Nishihama, Ryuichi
last_name: Nishihama
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Joris
full_name: Sprakel, Joris
last_name: Sprakel
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
citation:
ama: Kuhn A, Roosjen M, Mutte S, et al. RAF-like protein kinases mediate a deeply
conserved, rapid auxin response. Cell. 2024;187(1):130-148.e17. doi:10.1016/j.cell.2023.11.021
apa: Kuhn, A., Roosjen, M., Mutte, S., Dubey, S. M., Carrillo Carrasco, V. P., Boeren,
S., … Weijers, D. (2024). RAF-like protein kinases mediate a deeply conserved,
rapid auxin response. Cell. Elsevier. https://doi.org/10.1016/j.cell.2023.11.021
chicago: Kuhn, Andre, Mark Roosjen, Sumanth Mutte, Shiv Mani Dubey, Vanessa Polet
Carrillo Carrasco, Sjef Boeren, Aline Monzer, et al. “RAF-like Protein Kinases
Mediate a Deeply Conserved, Rapid Auxin Response.” Cell. Elsevier, 2024.
https://doi.org/10.1016/j.cell.2023.11.021.
ieee: A. Kuhn et al., “RAF-like protein kinases mediate a deeply conserved,
rapid auxin response,” Cell, vol. 187, no. 1. Elsevier, p. 130–148.e17,
2024.
ista: Kuhn A, Roosjen M, Mutte S, Dubey SM, Carrillo Carrasco VP, Boeren S, Monzer
A, Koehorst J, Kohchi T, Nishihama R, Fendrych M, Sprakel J, Friml J, Weijers
D. 2024. RAF-like protein kinases mediate a deeply conserved, rapid auxin response.
Cell. 187(1), 130–148.e17.
mla: Kuhn, Andre, et al. “RAF-like Protein Kinases Mediate a Deeply Conserved, Rapid
Auxin Response.” Cell, vol. 187, no. 1, Elsevier, 2024, p. 130–148.e17,
doi:10.1016/j.cell.2023.11.021.
short: A. Kuhn, M. Roosjen, S. Mutte, S.M. Dubey, V.P. Carrillo Carrasco, S. Boeren,
A. Monzer, J. Koehorst, T. Kohchi, R. Nishihama, M. Fendrych, J. Sprakel, J. Friml,
D. Weijers, Cell 187 (2024) 130–148.e17.
das_tickbox: '1'
dataavailabilitystatement: "Phosphoproteome data are available in the ProteomeXchange
PRIDE database: PXD038369 and PXD038363. Transcriptome data are available in the
NCBI Sequence Read Archive: PRJNA881051. Data underlying RAF-like kinase phylogeny
is deposited at the iTOL: https://itol.embl.de/shared/dolfweijers. This paper analyzes
existing, publicly available data phospho-proteome data. The accession number for
the datasets are listed in the key resources table.\r\n\r\nCode for the AuxPhos
app and for MATlab code for analysis of cytoplasmic streaming are available at Github:
https://github.com/WeijersLab/AuxPhos and https://github.com/jorissprakel/RAF_track_analysis.\r\n\r\nAny
additional information required to reanalyze the data reported in this paper is
available from the lead author upon request."
date_created: 2024-01-17T12:45:40Z
date_published: 2024-01-04T00:00:00Z
date_updated: 2026-10-15T08:06:16Z
day: '04'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.cell.2023.11.021
ec_funded: 1
external_id:
isi:
- '001152705700001'
pmid:
- '38128538'
file:
- access_level: open_access
checksum: 06fd236a9ee0b46ccb05f44695bfc34b
content_type: application/pdf
creator: dernst
date_created: 2024-01-22T13:41:41Z
date_updated: 2024-01-22T13:41:41Z
file_id: '14874'
file_name: 2024_Cell_Kuhn.pdf
file_size: 13194060
relation: main_file
success: 1
file_date_updated: 2024-01-22T13:41:41Z
fulldoi: https://doi.org/10.1016/j.cell.2023.11.021
has_accepted_license: '1'
intvolume: ' 187'
isi: 1
issue: '1'
keyword:
- General Biochemistry
- Genetics and Molecular Biology
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '01'
oa: 1
oa_version: Published Version
page: 130-148.e17
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Cell
publication_identifier:
eissn:
- 1097-4172
issn:
- 0092-8674
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '19395'
relation: dissertation_contains
status: public
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: RAF-like protein kinases mediate a deeply conserved, rapid auxin response
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 187
year: '2024'
...
---
_id: '15319'
abstract:
- lang: eng
text: Exocyst component of 70-kDa (EXO70) proteins are constituents of the exocyst
complex implicated in vesicle tethering during exocytosis. MILDEW RESISTANCE LOCUS
O (MLO) proteins are plant-specific calcium channels and some MLO isoforms enable
fungal powdery mildew pathogenesis. We here detected an unexpected phenotypic
overlap of Arabidopsis thaliana exo70H4 and mlo2 mlo6 mlo12 triple mutant plants
regarding the biogenesis of leaf trichome secondary cell walls. Biochemical and
Fourier transform infrared spectroscopic analyses corroborated deficiencies in
the composition of trichome cell walls in these mutants. Transgenic lines expressing
fluorophore-tagged EXO70H4 and MLO exhibited extensive colocalization of these
proteins. Furthermore, mCherry-EXO70H4 mislocalized in trichomes of the mlo triple
mutant and, vice versa, MLO6-GFP mislocalized in trichomes of the exo70H4 mutant.
Expression of GFP-marked PMR4 callose synthase, a known cargo of EXO70H4-dependent
exocytosis, revealed reduced cell wall delivery of GFP-PMR4 in trichomes of mlo
triple mutant plants. In vivo protein–protein interaction assays in plant and
yeast cells uncovered isoform-preferential interactions between EXO70.2 subfamily
members and MLO proteins. Finally, exo70H4 and mlo6 mutants, when combined, showed
synergistically enhanced resistance to powdery mildew attack. Taken together,
our data point to an isoform-specific interplay of EXO70 and MLO proteins in the
modulation of trichome cell wall biogenesis and powdery mildew susceptibility.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Jan W.
full_name: Huebbers, Jan W.
last_name: Huebbers
- first_name: George A.
full_name: Caldarescu, George A.
last_name: Caldarescu
- first_name: Zdeňka
full_name: Kubátová, Zdeňka
last_name: Kubátová
- first_name: Peter
full_name: Sabol, Peter
last_name: Sabol
- first_name: Sophie C.J.
full_name: Levecque, Sophie C.J.
last_name: Levecque
- first_name: Hannah
full_name: Kuhn, Hannah
last_name: Kuhn
- first_name: Ivan
full_name: Kulich, Ivan
id: 57a1567c-8314-11eb-9063-c9ddc3451a54
last_name: Kulich
- first_name: Anja
full_name: Reinstädler, Anja
last_name: Reinstädler
- first_name: Kim
full_name: Büttgen, Kim
last_name: Büttgen
- first_name: Alba
full_name: Manga-Robles, Alba
last_name: Manga-Robles
- first_name: Hugo
full_name: Mélida, Hugo
last_name: Mélida
- first_name: Markus
full_name: Pauly, Markus
last_name: Pauly
- first_name: Ralph
full_name: Panstruga, Ralph
last_name: Panstruga
- first_name: Viktor
full_name: Žárský, Viktor
last_name: Žárský
citation:
ama: Huebbers JW, Caldarescu GA, Kubátová Z, et al. Interplay of EXO70 and MLO proteins
modulates trichome cell wall composition and susceptibility to powdery mildew.
Plant Cell. 2024;36(4):1007-1035. doi:10.1093/plcell/koad319
apa: Huebbers, J. W., Caldarescu, G. A., Kubátová, Z., Sabol, P., Levecque, S. C.
J., Kuhn, H., … Žárský, V. (2024). Interplay of EXO70 and MLO proteins modulates
trichome cell wall composition and susceptibility to powdery mildew. Plant
Cell. Oxford University Press. https://doi.org/10.1093/plcell/koad319
chicago: Huebbers, Jan W., George A. Caldarescu, Zdeňka Kubátová, Peter Sabol, Sophie
C.J. Levecque, Hannah Kuhn, Ivan Kulich, et al. “Interplay of EXO70 and MLO Proteins
Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew.”
Plant Cell. Oxford University Press, 2024. https://doi.org/10.1093/plcell/koad319.
ieee: J. W. Huebbers et al., “Interplay of EXO70 and MLO proteins modulates
trichome cell wall composition and susceptibility to powdery mildew,” Plant
Cell, vol. 36, no. 4. Oxford University Press, pp. 1007–1035, 2024.
ista: Huebbers JW, Caldarescu GA, Kubátová Z, Sabol P, Levecque SCJ, Kuhn H, Kulich
I, Reinstädler A, Büttgen K, Manga-Robles A, Mélida H, Pauly M, Panstruga R, Žárský
V. 2024. Interplay of EXO70 and MLO proteins modulates trichome cell wall composition
and susceptibility to powdery mildew. Plant Cell. 36(4), 1007–1035.
mla: Huebbers, Jan W., et al. “Interplay of EXO70 and MLO Proteins Modulates Trichome
Cell Wall Composition and Susceptibility to Powdery Mildew.” Plant Cell,
vol. 36, no. 4, Oxford University Press, 2024, pp. 1007–35, doi:10.1093/plcell/koad319.
short: J.W. Huebbers, G.A. Caldarescu, Z. Kubátová, P. Sabol, S.C.J. Levecque, H.
Kuhn, I. Kulich, A. Reinstädler, K. Büttgen, A. Manga-Robles, H. Mélida, M. Pauly,
R. Panstruga, V. Žárský, Plant Cell 36 (2024) 1007–1035.
das_tickbox: '1'
dataavailabilitystatement: All relevant data can be found within the manuscript and
its supporting materials.
date_created: 2024-04-14T22:01:02Z
date_published: 2024-04-01T00:00:00Z
date_updated: 2026-10-15T08:09:23Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plcell/koad319
external_id:
pmid:
- '38124479'
file:
- access_level: open_access
checksum: f9994d2e4748feec24c992b39871aba1
content_type: application/pdf
creator: dernst
date_created: 2024-04-17T06:57:54Z
date_updated: 2024-04-17T06:57:54Z
file_id: '15326'
file_name: 2024_PlantCell_Huebbers.pdf
file_size: 2866098
relation: main_file
success: 1
file_date_updated: 2024-04-17T06:57:54Z
fulldoi: https://doi.org/10.1093/plcell/koad319
has_accepted_license: '1'
intvolume: ' 36'
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 1007-1035
pmid: 1
publication: Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
researchdata_availability: yes (supplementary data)
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Interplay of EXO70 and MLO proteins modulates trichome cell wall composition
and susceptibility to powdery mildew
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 36
year: '2024'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '17436'
abstract:
- lang: eng
text: The auxin signaling molecule controls a variety of growth and developmental
processes in land plants. Auxin regulates gene expression through a nuclear auxin
signaling pathway (NAP) consisting of the ubiquitin ligase auxin receptor TIR1/AFB,
its Aux/IAA degradation substrate, and DNA-binding ARF transcription factors.
Although extensive qualitative understanding of the pathway and its interactions
has been obtained, mostly by studying the flowering plant Arabidopsis thaliana,
it remains unknown how these translate to quantitative system behavior in vivo,
a problem that is confounded by the large NAP gene families in most species. Here,
we used the minimal NAP of the liverwort Marchantia polymorpha to quantitatively
map NAP protein accumulation and dynamics in vivo through the use of knockin fluorescent
fusion proteins. Beyond revealing the dynamic native accumulation profile of the
entire NAP protein network, we discovered that the two central ARFs, MpARF1 and
MpARF2, are proteasomally degraded. This auxin-independent degradation tunes ARF
protein stoichiometry to favor gene activation, thereby reprogramming auxin response
during the developmental progression. Thus, quantitative analysis of the entire
NAP has enabled us to identify ARF degradation and the stoichiometries of activator
and repressor ARFs as a potential mechanism for controlling gemma germination.
acknowledgement: "We are grateful to Iris Nieuwland and Neri van Laar for experimental
support. No conflict of interest declared.\r\nThis work was supported by the Netherlands
Organisation for Scientific Research, the Netherlands (grants ALWOP.402 and OCENW.M20.031
to J.W.B.) and the Human Frontiers Research Program (grant RGP0015/2022 to D.W.)."
article_number: '101039'
article_processing_charge: Yes
article_type: original
author:
- first_name: Shubhajit
full_name: Das, Shubhajit
id: b08969a4-f2a5-11ed-b6c4-ff0f10b7d0be
last_name: Das
- first_name: Martijn
full_name: De Roij, Martijn
last_name: De Roij
- first_name: Simon
full_name: Bellows, Simon
last_name: Bellows
- first_name: Melissa Dipp
full_name: Alvarez, Melissa Dipp
last_name: Alvarez
- first_name: Sumanth
full_name: Mutte, Sumanth
last_name: Mutte
- first_name: Wouter
full_name: Kohlen, Wouter
last_name: Kohlen
- first_name: Etienne
full_name: Farcot, Etienne
last_name: Farcot
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Jan Willem
full_name: Borst, Jan Willem
last_name: Borst
citation:
ama: Das S, De Roij M, Bellows S, et al. Quantitative imaging reveals the role of
MpARF proteasomal degradation during gemma germination. Plant Communications.
2024;5(11). doi:10.1016/j.xplc.2024.101039
apa: Das, S., De Roij, M., Bellows, S., Alvarez, M. D., Mutte, S., Kohlen, W., …
Borst, J. W. (2024). Quantitative imaging reveals the role of MpARF proteasomal
degradation during gemma germination. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2024.101039
chicago: Das, Shubhajit, Martijn De Roij, Simon Bellows, Melissa Dipp Alvarez, Sumanth
Mutte, Wouter Kohlen, Etienne Farcot, Dolf Weijers, and Jan Willem Borst. “Quantitative
Imaging Reveals the Role of MpARF Proteasomal Degradation during Gemma Germination.”
Plant Communications. Elsevier, 2024. https://doi.org/10.1016/j.xplc.2024.101039.
ieee: S. Das et al., “Quantitative imaging reveals the role of MpARF proteasomal
degradation during gemma germination,” Plant Communications, vol. 5, no.
11. Elsevier, 2024.
ista: Das S, De Roij M, Bellows S, Alvarez MD, Mutte S, Kohlen W, Farcot E, Weijers
D, Borst JW. 2024. Quantitative imaging reveals the role of MpARF proteasomal
degradation during gemma germination. Plant Communications. 5(11), 101039.
mla: Das, Shubhajit, et al. “Quantitative Imaging Reveals the Role of MpARF Proteasomal
Degradation during Gemma Germination.” Plant Communications, vol. 5, no.
11, 101039, Elsevier, 2024, doi:10.1016/j.xplc.2024.101039.
short: S. Das, M. De Roij, S. Bellows, M.D. Alvarez, S. Mutte, W. Kohlen, E. Farcot,
D. Weijers, J.W. Borst, Plant Communications 5 (2024).
das_tickbox: '0'
date_created: 2024-08-18T22:01:04Z
date_published: 2024-11-11T00:00:00Z
date_updated: 2026-10-15T08:12:01Z
day: '11'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2024.101039
external_id:
pmid:
- '38988072'
file:
- access_level: open_access
checksum: 38cabc1042ac7fb70e6c4c510eba88fc
content_type: application/pdf
creator: dernst
date_created: 2025-01-09T12:25:32Z
date_updated: 2025-01-09T12:25:32Z
file_id: '18811'
file_name: 2024_PlantCommunications_Das.pdf
file_size: 4970540
relation: main_file
success: 1
file_date_updated: 2025-01-09T12:25:32Z
fulldoi: https://doi.org/10.1016/j.xplc.2024.101039
has_accepted_license: '1'
intvolume: ' 5'
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Communications
publication_identifier:
eissn:
- 2590-3462
publication_status: published
publisher: Elsevier
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Quantitative imaging reveals the role of MpARF proteasomal degradation during
gemma germination
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 5
year: '2024'
...
---
OA_place: publisher
OA_type: hybrid
_id: '18465'
abstract:
- lang: eng
text: The phytohormone auxin is polarly transported in plants by PIN-FORMED (PIN)
transporters and controls virtually all growth and developmental processes. Canonical
PINs possess a long, largely disordered cytosolic loop. Auxin transport by canonical
PINs is activated by loop phosphorylation by certain kinases. The structure of
the PIN transmembrane domains was recently determined, their transport properties
remained poorly characterized, and the role of the loop in the transport process
was unclear. Here, we determined the quantitative kinetic parameters of auxin
transport mediated by Arabidopsis PINs to mathematically model auxin distribution
in roots and to test these predictions in vivo. Using chimeras between transmembrane
and loop domains of different PINs, we demonstrate a strong correlation between
transport parameters and physiological output, indicating that the loop domain
is not only required to activate PIN-mediated auxin transport, but it has an additional
role in the transport process by a currently unknown mechanism.
acknowledgement: This work was funded by DFG3468/6-1, DFG3468/6-3, and SFB924 to U.Z.H.
We thank Angela Alkofer and Helene Prunkl for excellent technical assistance and
Xenopus maintenance. Christian Luschnig is acknowledged for sharing unpublished
results and valuable discussions.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: DP
full_name: Janacek, DP
last_name: Janacek
- first_name: M
full_name: Kolb, M
last_name: Kolb
- first_name: L
full_name: Schulz, L
last_name: Schulz
- first_name: J
full_name: Mergner, J
last_name: Mergner
- first_name: B
full_name: Kuster, B
last_name: Kuster
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: K
full_name: Ten Tusscher, K
last_name: Ten Tusscher
- first_name: C
full_name: Schwechheimer, C
last_name: Schwechheimer
- first_name: UZ
full_name: Hammes, UZ
last_name: Hammes
citation:
ama: Janacek D, Kolb M, Schulz L, et al. Transport properties of canonical PIN-FORMED
proteins from Arabidopsis and the role of the loop domain in auxin transport.
Developmental Cell. 2024;59(14):S1534-5807(24)00569-0. doi:10.1016/j.devcel.2024.09.020
apa: Janacek, D., Kolb, M., Schulz, L., Mergner, J., Kuster, B., Glanc, M., … Hammes,
U. (2024). Transport properties of canonical PIN-FORMED proteins from Arabidopsis
and the role of the loop domain in auxin transport. Developmental Cell.
Elsevier. https://doi.org/10.1016/j.devcel.2024.09.020
chicago: Janacek, DP, M Kolb, L Schulz, J Mergner, B Kuster, Matous Glanc, Jiří
Friml, K Ten Tusscher, C Schwechheimer, and UZ Hammes. “Transport Properties of
Canonical PIN-FORMED Proteins from Arabidopsis and the Role of the Loop Domain
in Auxin Transport.” Developmental Cell. Elsevier, 2024. https://doi.org/10.1016/j.devcel.2024.09.020.
ieee: D. Janacek et al., “Transport properties of canonical PIN-FORMED proteins
from Arabidopsis and the role of the loop domain in auxin transport,” Developmental
Cell, vol. 59, no. 14. Elsevier, pp. S1534-5807(24)00569–0, 2024.
ista: Janacek D, Kolb M, Schulz L, Mergner J, Kuster B, Glanc M, Friml J, Ten Tusscher
K, Schwechheimer C, Hammes U. 2024. Transport properties of canonical PIN-FORMED
proteins from Arabidopsis and the role of the loop domain in auxin transport.
Developmental Cell. 59(14), S1534-5807(24)00569–0.
mla: Janacek, DP, et al. “Transport Properties of Canonical PIN-FORMED Proteins
from Arabidopsis and the Role of the Loop Domain in Auxin Transport.” Developmental
Cell, vol. 59, no. 14, Elsevier, 2024, pp. S1534-5807(24)00569-0, doi:10.1016/j.devcel.2024.09.020.
short: D. Janacek, M. Kolb, L. Schulz, J. Mergner, B. Kuster, M. Glanc, J. Friml,
K. Ten Tusscher, C. Schwechheimer, U. Hammes, Developmental Cell 59 (2024) S1534-5807(24)00569–0.
das_tickbox: '1'
dataavailabilitystatement: "The mass spectrometry proteomics data have been deposited
to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset
identifier PXD044850 (user name: reviewer_pxd044850@ebi.ac.uk, password: 3xBdgLSX).59\r\n\r\nThis
paper does not report original codes.\r\n\r\nAny additional information required
to reanalyze the data reported in this paper is available from the lead contact
upon request."
date_created: 2024-10-23T08:41:27Z
date_published: 2024-12-16T00:00:00Z
date_updated: 2026-10-15T08:13:11Z
day: '16'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1016/j.devcel.2024.09.020
external_id:
isi:
- '001390774300001'
pmid:
- '39413780'
file:
- access_level: open_access
checksum: 34423ee9fb4e30334f3572eddf1da2ae
content_type: application/pdf
creator: dernst
date_created: 2025-01-13T09:20:15Z
date_updated: 2025-01-13T09:20:15Z
file_id: '18835'
file_name: 2024_DevelopmentalCell_Janacek.pdf
file_size: 3675955
relation: main_file
success: 1
file_date_updated: 2025-01-13T09:20:15Z
fulldoi: https://doi.org/10.1016/j.devcel.2024.09.020
has_accepted_license: '1'
intvolume: ' 59'
isi: 1
issue: '14'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: S1534-5807(24)00569-0
pmid: 1
publication: Developmental Cell
publication_identifier:
eissn:
- 1878-1551
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Transport properties of canonical PIN-FORMED proteins from Arabidopsis and
the role of the loop domain in auxin transport
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 59
year: '2024'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18582'
abstract:
- lang: eng
text: Identification of PIN exporters for auxin, the major coordinative signal in
plants, some 25 years ago, signifies a landmark in our understanding of plant-specific
mechanisms underlying development and adaptation. Auxin is directionally transported
throughout the plant body; a unique feature already envisioned by Darwin and solidified
by PINs’ discovery and characterization. The PIN-based auxin distribution network
with its complex regulations of PIN expression, localization and activity turned
out to underlie a remarkable multitude of developmental processes and represents
means to integrate endogenous and environmental signals. Given the recent anniversary,
we here summarize past and current developments in this exciting field.
acknowledgement: We gratefully acknowledge Leo Gälweiler for authorizing his PIN1
story. We would like to thank Yuanrong Pei for invaluable help with preparing figures.
Work in the lab of C.L. is supported by grants from the Austrian Science Fund (PAT
8419423) and by the Gesellschaft für Forschungsförderung Niederösterreich m.b.H.
(FTI19-008). The lab of J.F. is supported by the Austrian Science Fund (I 6123-B
and P 37051-B).
article_number: '9904'
article_processing_charge: Yes
article_type: original
author:
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Luschnig C, Friml J. Over 25 years of decrypting PIN-mediated plant development.
Nature Communications. 2024;15. doi:10.1038/s41467-024-54240-y
apa: Luschnig, C., & Friml, J. (2024). Over 25 years of decrypting PIN-mediated
plant development. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-024-54240-y
chicago: Luschnig, Christian, and Jiří Friml. “Over 25 Years of Decrypting PIN-Mediated
Plant Development.” Nature Communications. Springer Nature, 2024. https://doi.org/10.1038/s41467-024-54240-y.
ieee: C. Luschnig and J. Friml, “Over 25 years of decrypting PIN-mediated plant
development,” Nature Communications, vol. 15. Springer Nature, 2024.
ista: Luschnig C, Friml J. 2024. Over 25 years of decrypting PIN-mediated plant
development. Nature Communications. 15, 9904.
mla: Luschnig, Christian, and Jiří Friml. “Over 25 Years of Decrypting PIN-Mediated
Plant Development.” Nature Communications, vol. 15, 9904, Springer Nature,
2024, doi:10.1038/s41467-024-54240-y.
short: C. Luschnig, J. Friml, Nature Communications 15 (2024).
corr_author: '1'
das_tickbox: '0'
date_created: 2024-11-24T23:01:48Z
date_published: 2024-12-01T00:00:00Z
date_updated: 2026-10-15T08:10:07Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-024-54240-y
external_id:
isi:
- '001356232600004'
pmid:
- '39548100'
file:
- access_level: open_access
checksum: 3a31af06f52100d287f1e9d9c2aa1d40
content_type: application/pdf
creator: dernst
date_created: 2024-12-03T14:10:54Z
date_updated: 2024-12-03T14:10:54Z
file_id: '18615'
file_name: 2024_NatureComm_Luschnig.pdf
file_size: 1426555
relation: main_file
success: 1
file_date_updated: 2024-12-03T14:10:54Z
fulldoi: https://doi.org/10.1038/s41467-024-54240-y
has_accepted_license: '1'
intvolume: ' 15'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: no
title: Over 25 years of decrypting PIN-mediated plant development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2024'
...
---
OA_place: repository
OA_type: green
_id: '15251'
abstract:
- lang: eng
text: Brassinosteroids are steroidal phytohormones that regulate plant development
and physiology, including adaptation to environmental stresses. Brassinosteroids
are synthesized in the cell interior but bind receptors at the cell surface, necessitating
a yet to be identified export mechanism. Here, we show that a member of the ATP-binding
cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid
exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound
states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis
activity, and transport assays showed that ABCB19 transports brassinosteroids.
In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate
brassinosteroid responses. Our results uncover an elusive export mechanism for
bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.
acknowledgement: "We thank the Cryo-EM Center of the University of Science and Technology
of China for the EM facility support, Y. Yin (Iowa State University, Ames, IA, USA)
for providing the anti-BES1 antibody, Y. Gao and all other staff members for their
technical support on cryo-EM data collection, S. Vanneste (VIB, Ghent University,
Ghent, Belgium) for useful discussions, and M. De Cock for help in preparing the
manuscript.\r\nThis work was supported by the National Natural Science Foundation
of China (grants 31870732 and 32322041 to L.S., grant 31900885 to X.L., and grant
32321001 to L.S.); the Strategic Priority Research Program of the Chinese Academy
of Sciences (grant XDB37020103 to L.S.); the Natural Science Foundation of Anhui
Province (grant 2008085MC90 to X.L. and grant 2008085J15 to L.S.); Fundamental Research
Funds for the Central Universities (grant WK9100000031 to L.S.); USTC Research Funds
of the Double First-Class Initiative (grant YD9100002004 to L.S. and grant YD9100002020
to X.L.); Research Foundation-Flanders (grant G002121N to E.R. and postdoctoral
fellowships 12R7822N and 12R7819N to N.V.); and Chinese Scholarship Council predoctoral
fellowships (Y.W. and H.Z.). L.S. is supported by an Outstanding Young Scholar Award
from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from
the Cyrus Tang Foundation."
article_processing_charge: No
article_type: original
author:
- first_name: Wei
full_name: Ying, Wei
last_name: Ying
- first_name: Yaowei
full_name: Wang, Yaowei
last_name: Wang
- first_name: Hong
full_name: Wei, Hong
last_name: Wei
- first_name: Yongming
full_name: Luo, Yongming
last_name: Luo
- first_name: Qian
full_name: Ma, Qian
last_name: Ma
- first_name: Heyuan
full_name: Zhu, Heyuan
last_name: Zhu
- first_name: Hilde
full_name: Janssens, Hilde
last_name: Janssens
- first_name: Nemanja
full_name: Vukašinović, Nemanja
last_name: Vukašinović
- first_name: Miroslav
full_name: Kvasnica, Miroslav
last_name: Kvasnica
- first_name: Johan M.
full_name: Winne, Johan M.
last_name: Winne
- first_name: Yongxiang
full_name: Gao, Yongxiang
last_name: Gao
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Xin
full_name: Liu, Xin
last_name: Liu
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Linfeng
full_name: Sun, Linfeng
last_name: Sun
citation:
ama: Ying W, Wang Y, Wei H, et al. Structure and function of the Arabidopsis ABC
transporter ABCB19 in brassinosteroid export. Science. 2024;383(6689):eadj4591.
doi:10.1126/science.adj4591
apa: Ying, W., Wang, Y., Wei, H., Luo, Y., Ma, Q., Zhu, H., … Sun, L. (2024). Structure
and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export.
Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.adj4591
chicago: Ying, Wei, Yaowei Wang, Hong Wei, Yongming Luo, Qian Ma, Heyuan Zhu, Hilde
Janssens, et al. “Structure and Function of the Arabidopsis ABC Transporter ABCB19
in Brassinosteroid Export.” Science. American Association for the Advancement
of Science, 2024. https://doi.org/10.1126/science.adj4591.
ieee: W. Ying et al., “Structure and function of the Arabidopsis ABC transporter
ABCB19 in brassinosteroid export,” Science, vol. 383, no. 6689. American
Association for the Advancement of Science, p. eadj4591, 2024.
ista: Ying W, Wang Y, Wei H, Luo Y, Ma Q, Zhu H, Janssens H, Vukašinović N, Kvasnica
M, Winne JM, Gao Y, Tan S, Friml J, Liu X, Russinova E, Sun L. 2024. Structure
and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export.
Science. 383(6689), eadj4591.
mla: Ying, Wei, et al. “Structure and Function of the Arabidopsis ABC Transporter
ABCB19 in Brassinosteroid Export.” Science, vol. 383, no. 6689, American
Association for the Advancement of Science, 2024, p. eadj4591, doi:10.1126/science.adj4591.
short: W. Ying, Y. Wang, H. Wei, Y. Luo, Q. Ma, H. Zhu, H. Janssens, N. Vukašinović,
M. Kvasnica, J.M. Winne, Y. Gao, S. Tan, J. Friml, X. Liu, E. Russinova, L. Sun,
Science 383 (2024) eadj4591.
das_tickbox: '1'
dataavailabilitystatement: All data are available in the main text or the supplementary
materials. Model coordinates and cryo-EM density maps for the WT ABCB19 structures
in the substrate-unbound state, BL-bound state, BL and AMP-PNP-bound state, and
the E529Q/E1174Q mutant ABCB19 structure in the ATP-bound state have been deposited
in the Protein Data Bank with accession codes 8WOI, 8WOM, 8WOO, and 8WP0, respectively,
and in the Electron Microscopy Data Bank with accession codes EMD-37690, EMD-37692,
EMD-37694, and EMD-37705, respectively.
date_created: 2024-03-31T22:01:12Z
date_published: 2024-03-22T00:00:00Z
date_updated: 2026-10-15T08:08:30Z
day: '22'
department:
- _id: JiFr
doi: 10.1126/science.adj4591
external_id:
isi:
- '001252955200028'
pmid:
- '38513023'
fulldoi: https://doi.org/10.1126/science.adj4591
intvolume: ' 383'
isi: 1
issue: '6689'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://hdl.handle.net/1854/LU-01HTMBK3P3PPNB73YFCMFXP3ZZ
month: '03'
oa: 1
oa_version: Submitted Version
page: eadj4591
pmid: 1
publication: Science
publication_identifier:
eissn:
- 1095-9203
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid
export
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 383
year: '2024'
...
---
OA_place: repository
OA_type: green
_id: '17377'
abstract:
- lang: eng
text: "Lateral root (LR) formation, that is vital for plant development, is one
of many auxin-modulated processes, but the underlying regulatory mechanism is
not yet fully known. Recently, \r\nGonzález-García et al. discovered the BiAux
compound and showed that it is involved in LR development via regulating specific
auxin coreceptors."
acknowledgement: "This work was supported by funding from the National Science Centre,
Poland, under the OPUS call in the Weave program and the Austrian Science Fund,
Austria project (FWF)/OPUS – Peptide receptor complexes for auxin canalization and
regeneration in Arabidopsis (grant \r\n2021/43/I/NZ1/01835) to E.M., and grant I
6123-B to J.F."
article_processing_charge: No
article_type: original
author:
- first_name: Barbara
full_name: Wójcikowska, Barbara
last_name: Wójcikowska
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
citation:
ama: Wójcikowska B, Friml J, Mazur E. BiAux, a newly discovered compound triggering
auxin signaling. Trends in Plant Science. 2024;29(12):1279-1281. doi:10.1016/j.tplants.2024.07.008
apa: Wójcikowska, B., Friml, J., & Mazur, E. (2024). BiAux, a newly discovered
compound triggering auxin signaling. Trends in Plant Science. Elsevier.
https://doi.org/10.1016/j.tplants.2024.07.008
chicago: Wójcikowska, Barbara, Jiří Friml, and Ewa Mazur. “BiAux, a Newly Discovered
Compound Triggering Auxin Signaling.” Trends in Plant Science. Elsevier,
2024. https://doi.org/10.1016/j.tplants.2024.07.008.
ieee: B. Wójcikowska, J. Friml, and E. Mazur, “BiAux, a newly discovered compound
triggering auxin signaling,” Trends in Plant Science, vol. 29, no. 12.
Elsevier, pp. 1279–1281, 2024.
ista: Wójcikowska B, Friml J, Mazur E. 2024. BiAux, a newly discovered compound
triggering auxin signaling. Trends in Plant Science. 29(12), 1279–1281.
mla: Wójcikowska, Barbara, et al. “BiAux, a Newly Discovered Compound Triggering
Auxin Signaling.” Trends in Plant Science, vol. 29, no. 12, Elsevier, 2024,
pp. 1279–81, doi:10.1016/j.tplants.2024.07.008.
short: B. Wójcikowska, J. Friml, E. Mazur, Trends in Plant Science 29 (2024) 1279–1281.
corr_author: '1'
das_tickbox: '0'
date_created: 2024-08-04T22:01:22Z
date_published: 2024-12-01T00:00:00Z
date_updated: 2026-10-15T08:27:18Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.tplants.2024.07.008
external_id:
isi:
- '001375335200001'
pmid:
- '39079770'
file:
- access_level: open_access
checksum: 89a83fe25b9ec1aad8e22d134a342c09
content_type: application/pdf
creator: dernst
date_created: 2025-11-24T15:12:50Z
date_updated: 2025-11-24T15:12:50Z
file_id: '20691'
file_name: 2024_TrendsPlantScience_Wojcikowska_submittedversion.pdf
file_size: 277636
relation: main_file
success: 1
file_date_updated: 2025-11-24T15:12:50Z
fulldoi: https://doi.org/10.1016/j.tplants.2024.07.008
has_accepted_license: '1'
intvolume: ' 29'
isi: 1
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Submitted Version
page: 1279-1281
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
publication: Trends in Plant Science
publication_identifier:
issn:
- 1360-1385
publication_status: published
publisher: Elsevier
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: no
title: BiAux, a newly discovered compound triggering auxin signaling
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 29
year: '2024'
...
---
_id: '15361'
abstract:
- lang: eng
text: Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying
protein-protein interactions in living cells. By fusing interacting proteins to
fluorescent protein fragments, BiFC allows visualization of spatial localization
patterns of protein complexes. This method has been adapted to a variety of expression
systems in different organisms and is widely used to study protein interactions
in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC
assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but
in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts
is presented.
acknowledgement: Special thanks to Dr. Marta Zwiewka for the support. Thanks to the
Czech Science Foundation GA 20-20860Y for financial aid and support of A.S.S., respectively.
Thanks go to Core Facility Cellular Imaging (CELLIM), and Plant Sciences Core Facility
of CEITEC Masaryk University is acknowledged for the technical support.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Aswathy
full_name: Jayasree, Aswathy
last_name: Jayasree
- first_name: Hymavathi
full_name: Salava, Hymavathi
last_name: Salava
- first_name: Tomasz
full_name: Nodzynski, Tomasz
last_name: Nodzynski
- first_name: Thula
full_name: Sravankumar, Thula
id: 055b7938-0b72-11ef-94eb-d14136011bb5
last_name: Sravankumar
orcid: 0000-0001-6925-6950
citation:
ama: 'Jayasree A, Salava H, Nodzynski T, Sravankumar T. Protein-Protein Interactions
Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana
Protoplasts from Leaf. In: Maghuly F, ed. Plant Functional Genomics. Vol
2787. MIMB. Springer Nature; 2024:305-313. doi:10.1007/978-1-0716-3778-4_21'
apa: Jayasree, A., Salava, H., Nodzynski, T., & Sravankumar, T. (2024). Protein-Protein
Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis
thaliana Protoplasts from Leaf. In F. Maghuly (Ed.), Plant Functional Genomics
(Vol. 2787, pp. 305–313). Springer Nature. https://doi.org/10.1007/978-1-0716-3778-4_21
chicago: Jayasree, Aswathy, Hymavathi Salava, Tomasz Nodzynski, and Thula Sravankumar.
“Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation
in Arabidopsis Thaliana Protoplasts from Leaf.” In Plant Functional Genomics,
edited by Fatemeh Maghuly, 2787:305–13. MIMB. Springer Nature, 2024. https://doi.org/10.1007/978-1-0716-3778-4_21.
ieee: A. Jayasree, H. Salava, T. Nodzynski, and T. Sravankumar, “Protein-Protein
Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis
thaliana Protoplasts from Leaf,” in Plant Functional Genomics, vol. 2787,
F. Maghuly, Ed. Springer Nature, 2024, pp. 305–313.
ista: 'Jayasree A, Salava H, Nodzynski T, Sravankumar T. 2024.Protein-Protein Interactions
Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana
Protoplasts from Leaf. In: Plant Functional Genomics. Methods in Molecular Biology,
vol. 2787, 305–313.'
mla: Jayasree, Aswathy, et al. “Protein-Protein Interactions Visualized by Bimolecular
Fluorescence Complementation in Arabidopsis Thaliana Protoplasts from Leaf.” Plant
Functional Genomics, edited by Fatemeh Maghuly, vol. 2787, Springer Nature,
2024, pp. 305–13, doi:10.1007/978-1-0716-3778-4_21.
short: A. Jayasree, H. Salava, T. Nodzynski, T. Sravankumar, in:, F. Maghuly (Ed.),
Plant Functional Genomics, Springer Nature, 2024, pp. 305–313.
das_tickbox: '0'
date_created: 2024-05-05T22:01:04Z
date_published: 2024-04-25T00:00:00Z
date_updated: 2026-10-15T08:09:43Z
day: '25'
department:
- _id: JiFr
doi: 10.1007/978-1-0716-3778-4_21
editor:
- first_name: Fatemeh
full_name: Maghuly, Fatemeh
last_name: Maghuly
external_id:
pmid:
- '38656499'
fulldoi: https://doi.org/10.1007/978-1-0716-3778-4_21
intvolume: ' 2787'
language:
- iso: eng
month: '04'
oa_version: None
page: 305-313
pmid: 1
publication: Plant Functional Genomics
publication_identifier:
eissn:
- 1940-6029
isbn:
- '9781071637777'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
series_title: MIMB
status: public
supplementarymaterial: no
title: Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation
in Arabidopsis thaliana Protoplasts from Leaf
type: book_chapter
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 2787
year: '2024'
...
---
OA_type: closed access
_id: '18311'
abstract:
- lang: eng
text: Local wound signaling in plants informs the surrounding tissues about an injury
and initiates the regeneration process. In a recent paper published in Cell, Yang
and colleagues show the involvement of a single Pep family member from tomato
in wound signaling and how exogenous application of this regeneration factor enhances
transformation efficiency in crops.
article_processing_charge: No
article_type: original
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Hörmayer L, Friml J. Feeling the danger: Local wound signaling in plants.
Cell Research. 2024;34:761-762. doi:10.1038/s41422-024-01035-x'
apa: 'Hörmayer, L., & Friml, J. (2024). Feeling the danger: Local wound signaling
in plants. Cell Research. Springer Nature. https://doi.org/10.1038/s41422-024-01035-x'
chicago: 'Hörmayer, Lukas, and Jiří Friml. “Feeling the Danger: Local Wound Signaling
in Plants.” Cell Research. Springer Nature, 2024. https://doi.org/10.1038/s41422-024-01035-x.'
ieee: 'L. Hörmayer and J. Friml, “Feeling the danger: Local wound signaling in plants,”
Cell Research, vol. 34. Springer Nature, pp. 761–762, 2024.'
ista: 'Hörmayer L, Friml J. 2024. Feeling the danger: Local wound signaling in plants.
Cell Research. 34, 761–762.'
mla: 'Hörmayer, Lukas, and Jiří Friml. “Feeling the Danger: Local Wound Signaling
in Plants.” Cell Research, vol. 34, Springer Nature, 2024, pp. 761–62,
doi:10.1038/s41422-024-01035-x.'
short: L. Hörmayer, J. Friml, Cell Research 34 (2024) 761–762.
corr_author: '1'
das_tickbox: '0'
date_created: 2024-10-13T22:01:51Z
date_published: 2024-11-01T00:00:00Z
date_updated: 2026-10-15T08:23:58Z
day: '01'
department:
- _id: JiFr
doi: 10.1038/s41422-024-01035-x
external_id:
isi:
- '001326684200001'
pmid:
- '39354142'
fulldoi: https://doi.org/10.1038/s41422-024-01035-x
intvolume: ' 34'
isi: 1
language:
- iso: eng
month: '11'
oa_version: None
page: 761-762
pmid: 1
publication: Cell Research
publication_identifier:
eissn:
- 1748-7838
issn:
- 1001-0602
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: no
title: 'Feeling the danger: Local wound signaling in plants'
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 34
year: '2024'
...
---
DOAJ_listed: '1'
_id: '15257'
abstract:
- lang: eng
text: Root gravitropic bending represents a fundamental aspect of terrestrial plant
physiology. Gravity is perceived by sedimentation of starch-rich plastids (statoliths)
to the bottom of the central root cap cells. Following gravity perception, intercellular
auxin transport is redirected downwards leading to an asymmetric auxin accumulation
at the lower root side causing inhibition of cell expansion, ultimately resulting
in downwards bending. How gravity-induced statoliths repositioning is translated
into asymmetric auxin distribution remains unclear despite PIN auxin efflux carriers
and the Negative Gravitropic Response of roots (NGR) proteins polarize along statolith
sedimentation, thus providing a plausible mechanism for auxin flow redirection.
In this study, using a functional NGR1-GFP construct, we visualized the NGR1 localization
on the statolith surface and plasma membrane (PM) domains in close proximity to
the statoliths, correlating with their movements. We determined that NGR1 binding
to these PM domains is indispensable for NGR1 functionality and relies on cysteine
acylation and adjacent polybasic regions as well as on lipid and sterol PM composition.
Detailed timing of the early events following graviperception suggested that both
NGR1 repolarization and initial auxin asymmetry precede the visible PIN3 polarization.
This discrepancy motivated us to unveil a rapid, NGR-dependent translocation of
PIN-activating AGCVIII kinase D6PK towards lower PMs of gravity-perceiving cells,
thus providing an attractive model for rapid redirection of auxin fluxes following
gravistimulation.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: 'The research leading to these results has received funding from
the European Research Council (ERC) under the European Union’s Horizon 2020 research
and innovation programme grant agreement No 742985 and Austrian Science Fund (FWF):
I3630-775 B25 to J.F. This research was also supported by the Lab Support Facility
(LSF) and the Imaging and Optics Facility (IOF) of IST Austria, namely Tereza Bělinová
for her help with the imaging. JS was supported by FemTECH fellowship.'
article_number: '91523'
article_processing_charge: Yes
article_type: original
author:
- first_name: Ivan
full_name: Kulich, Ivan
id: 57a1567c-8314-11eb-9063-c9ddc3451a54
last_name: Kulich
- first_name: Julia
full_name: Schmid, Julia
id: 07cf4637-baaf-11ee-9227-e1de57d1d69b
last_name: Schmid
- first_name: Anastasiia
full_name: Teplova, Anastasiia
id: e3736151-106c-11ec-b916-c2558e2762c6
last_name: Teplova
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kulich I, Schmid J, Teplova A, Qi L, Friml J. Rapid translocation of NGR proteins
driving polarization of PIN-activating D6 protein kinase during root gravitropism.
eLife. 2024;12. doi:10.7554/elife.91523
apa: Kulich, I., Schmid, J., Teplova, A., Qi, L., & Friml, J. (2024). Rapid
translocation of NGR proteins driving polarization of PIN-activating D6 protein
kinase during root gravitropism. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.91523
chicago: Kulich, Ivan, Julia Schmid, Anastasiia Teplova, Linlin Qi, and Jiří Friml.
“Rapid Translocation of NGR Proteins Driving Polarization of PIN-Activating D6
Protein Kinase during Root Gravitropism.” ELife. eLife Sciences Publications,
2024. https://doi.org/10.7554/elife.91523.
ieee: I. Kulich, J. Schmid, A. Teplova, L. Qi, and J. Friml, “Rapid translocation
of NGR proteins driving polarization of PIN-activating D6 protein kinase during
root gravitropism,” eLife, vol. 12. eLife Sciences Publications, 2024.
ista: Kulich I, Schmid J, Teplova A, Qi L, Friml J. 2024. Rapid translocation of
NGR proteins driving polarization of PIN-activating D6 protein kinase during root
gravitropism. eLife. 12, 91523.
mla: Kulich, Ivan, et al. “Rapid Translocation of NGR Proteins Driving Polarization
of PIN-Activating D6 Protein Kinase during Root Gravitropism.” ELife, vol.
12, 91523, eLife Sciences Publications, 2024, doi:10.7554/elife.91523.
short: I. Kulich, J. Schmid, A. Teplova, L. Qi, J. Friml, ELife 12 (2024).
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: All data generated in this study are included as a manuscript
supporting files.
date_created: 2024-04-02T11:35:58Z
date_published: 2024-03-05T00:00:00Z
date_updated: 2026-10-15T08:30:04Z
day: '05'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.7554/elife.91523
ec_funded: 1
external_id:
pmid:
- '38441122'
file:
- access_level: open_access
checksum: a73a84d3bf97a6d09d24308ca6dd0a0c
content_type: application/pdf
creator: dernst
date_created: 2024-04-03T13:18:00Z
date_updated: 2024-04-03T13:18:00Z
file_id: '15288'
file_name: 2024_eLife_Kulich.pdf
file_size: 11451904
relation: main_file
success: 1
file_date_updated: 2024-04-03T13:18:00Z
fulldoi: https://doi.org/10.7554/elife.91523
has_accepted_license: '1'
intvolume: ' 12'
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/beneath-the-surface/
record:
- id: '22984'
relation: dissertation_contains
status: public
researchdata_availability: yes (supplementary data)
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Rapid translocation of NGR proteins driving polarization of PIN-activating
D6 protein kinase during root gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 12
year: '2024'
...
---
_id: '15301'
abstract:
- lang: eng
text: Plant morphogenesis relies exclusively on oriented cell expansion and division.
Nonetheless, the mechanism(s) determining division plane orientation remain elusive.
Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis
thaliana and uncovered how mechanical forces stabilize and reorient the microtubule
cytoskeleton for the orientation of cell division. We identified that root tissue
functions as an interconnected cell matrix, with a radial gradient of tissue extendibility
causing predictable tissue deformation after wounding. This deformation causes
instant redirection of expansion in the surrounding cells and reorientation of
microtubule arrays, ultimately predicting cell division orientation. Microtubules
are destabilized under low tension, whereas stretching of cells, either through
wounding or external aspiration, immediately induces their polymerization. The
higher microtubule abundance in the stretched cell parts leads to the reorientation
of microtubule arrays and, ultimately, informs cell division planes. This provides
a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical
forces for tissue reconstruction and plant architecture.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder
for sharing published material. We thank the Imaging & Optics and Life Science Facilities
at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility
at DBMV UNIL for providing invaluable assistance. The research leading to these
results has received funding from the European Research Council under the European
Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985,
from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: N
full_name: Trozzi, N
last_name: Trozzi
- first_name: Leonhard
full_name: Spona, Leonhard
id: b52391fb-f636-11ee-939c-8a8c47552e8a
last_name: Spona
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
orcid: 0000-0001-6111-9353
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Silvia
full_name: Caballero Mancebo, Silvia
id: 2F1E1758-F248-11E8-B48F-1D18A9856A87
last_name: Caballero Mancebo
orcid: 0000-0002-5223-3346
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Y
full_name: Dagdas, Y
last_name: Dagdas
- first_name: M
full_name: Majda, M
last_name: Majda
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant
tissue matrix orient cell divisions via microtubule stabilization. Developmental
Cell. 2024;59(10):1333-1344.e4. doi:10.1016/j.devcel.2024.03.009
apa: Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S.,
Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient
cell divisions via microtubule stabilization. Developmental Cell. Elsevier.
https://doi.org/10.1016/j.devcel.2024.03.009
chicago: Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko
Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in
Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” Developmental
Cell. Elsevier, 2024. https://doi.org/10.1016/j.devcel.2024.03.009.
ieee: L. Hörmayer et al., “Mechanical forces in plant tissue matrix orient
cell divisions via microtubule stabilization,” Developmental Cell, vol.
59, no. 10. Elsevier, p. 1333–1344.e4, 2024.
ista: Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P,
Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024.
Mechanical forces in plant tissue matrix orient cell divisions via microtubule
stabilization. Developmental Cell. 59(10), 1333–1344.e4.
mla: Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell
Divisions via Microtubule Stabilization.” Developmental Cell, vol. 59,
no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:10.1016/j.devcel.2024.03.009.
short: L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá,
S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J.
Friml, Developmental Cell 59 (2024) 1333–1344.e4.
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: "All data reported in this paper will be shared by the
lead contact upon request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny
additional information required to reanalyze the data reported in this paper is
available from the lead contact upon request."
date_created: 2024-04-08T12:07:57Z
date_published: 2024-05-20T00:00:00Z
date_updated: 2026-10-15T08:32:32Z
day: '20'
ddc:
- '570'
department:
- _id: JiFr
- _id: EvBe
- _id: CaHe
doi: 10.1016/j.devcel.2024.03.009
ec_funded: 1
external_id:
isi:
- '001301584600001'
pmid:
- '38579717'
file:
- access_level: open_access
checksum: 22b374fb50a40d380b7686c84258d271
content_type: application/pdf
creator: dernst
date_created: 2024-08-20T11:22:16Z
date_updated: 2024-08-20T11:22:16Z
file_id: '17452'
file_name: 2024_DevelopmentalCell_Hoermayer.pdf
file_size: 5195262
relation: main_file
success: 1
file_date_updated: 2024-08-20T11:22:16Z
fulldoi: https://doi.org/10.1016/j.devcel.2024.03.009
has_accepted_license: '1'
intvolume: ' 59'
isi: 1
issue: '10'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1333-1344.e4
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Developmental Cell
publication_identifier:
eissn:
- 1878-1551
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/how-plants-heal-wounds/
researchdata_availability: upon request
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Mechanical forces in plant tissue matrix orient cell divisions via microtubule
stabilization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 59
year: '2024'
...
---
OA_place: publisher
OA_type: hybrid
_id: '18596'
abstract:
- lang: eng
text: Hormone perception and signaling pathways have a fundamental regulatory function
in the physiological processes of plants. Cytokinins, a class of plant hormones,
regulate cell division and meristem maintenance. The cytokinin signaling pathway
is well established in the model plant Arabidopsis thaliana. Several negative
feedback mechanisms, tightly controlling cytokinin signaling output, have been
described previously. In this study, we identified a new feedback mechanism executed
through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing
variant named CRE1int7 results from seventh intron retention, introducing a premature
termination codon in the transcript. We showed that CRE1int7 is translated in
planta into a truncated receptor lacking the C-terminal receiver domain essential
for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream
cascade. We present a novel negative feedback mechanism of the cytokinin signaling
pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin
receptors via dimerization and compete with them for ligand binding. Ensuring
proper plant growth and development requires precise control of the cytokinin
signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism
for fine-tuning the cytokinin signaling pathway in Arabidopsis.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We dedicate this paper to the deceased Petr Galuszka for his inspiration
and support of our project. We thank Prof. Peter Hedden for constructive criticism
of the manuscript and English editing. No conflict of interest is declared.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Michaela
full_name: Králová, Michaela
last_name: Králová
- first_name: Ivona
full_name: Kubalová, Ivona
last_name: Kubalová
- first_name: Jakub
full_name: Hajný, Jakub
last_name: Hajný
- first_name: Karolina
full_name: Kubiasova, Karolina
id: 946011F4-3E71-11EA-860B-C7A73DDC885E
last_name: Kubiasova
orcid: 0000-0001-5630-9419
- first_name: Karolína
full_name: Vagaská, Karolína
last_name: Vagaská
- first_name: Zengxiang
full_name: Ge, Zengxiang
id: f43371a3-09ff-11eb-8013-bd0c6a2f6de8
last_name: Ge
orcid: 0000-0001-9381-3577
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Hana
full_name: Semerádová, Hana
id: 42FE702E-F248-11E8-B48F-1D18A9856A87
last_name: Semerádová
- first_name: Anna
full_name: Kuchařová, Anna
last_name: Kuchařová
- first_name: Martin
full_name: Hönig, Martin
last_name: Hönig
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Martin
full_name: Kovačik, Martin
last_name: Kovačik
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Yoshihisa
full_name: Ikeda, Yoshihisa
last_name: Ikeda
- first_name: David
full_name: Zalabák, David
last_name: Zalabák
citation:
ama: Králová M, Kubalová I, Hajný J, et al. A decoy receptor derived from alternative
splicing fine-tunes cytokinin signaling in Arabidopsis. Molecular Plant.
2024;17(12):1850-1865. doi:10.1016/j.molp.2024.11.001
apa: Králová, M., Kubalová, I., Hajný, J., Kubiasova, K., Vagaská, K., Ge, Z., …
Zalabák, D. (2024). A decoy receptor derived from alternative splicing fine-tunes
cytokinin signaling in Arabidopsis. Molecular Plant. Elsevier. https://doi.org/10.1016/j.molp.2024.11.001
chicago: Králová, Michaela, Ivona Kubalová, Jakub Hajný, Karolina Kubiasova, Karolína
Vagaská, Zengxiang Ge, Michelle C Gallei, et al. “A Decoy Receptor Derived from
Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” Molecular
Plant. Elsevier, 2024. https://doi.org/10.1016/j.molp.2024.11.001.
ieee: M. Králová et al., “A decoy receptor derived from alternative splicing
fine-tunes cytokinin signaling in Arabidopsis,” Molecular Plant, vol. 17,
no. 12. Elsevier, pp. 1850–1865, 2024.
ista: Králová M, Kubalová I, Hajný J, Kubiasova K, Vagaská K, Ge Z, Gallei MC, Semerádová
H, Kuchařová A, Hönig M, Monzer A, Kovačik M, Friml J, Novák O, Benková E, Ikeda
Y, Zalabák D. 2024. A decoy receptor derived from alternative splicing fine-tunes
cytokinin signaling in Arabidopsis. Molecular Plant. 17(12), 1850–1865.
mla: Králová, Michaela, et al. “A Decoy Receptor Derived from Alternative Splicing
Fine-Tunes Cytokinin Signaling in Arabidopsis.” Molecular Plant, vol. 17,
no. 12, Elsevier, 2024, pp. 1850–65, doi:10.1016/j.molp.2024.11.001.
short: M. Králová, I. Kubalová, J. Hajný, K. Kubiasova, K. Vagaská, Z. Ge, M.C.
Gallei, H. Semerádová, A. Kuchařová, M. Hönig, A. Monzer, M. Kovačik, J. Friml,
O. Novák, E. Benková, Y. Ikeda, D. Zalabák, Molecular Plant 17 (2024) 1850–1865.
das_tickbox: '0'
date_created: 2024-11-28T11:13:35Z
date_published: 2024-12-02T00:00:00Z
date_updated: 2026-10-15T08:30:20Z
day: '02'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.molp.2024.11.001
external_id:
isi:
- '001373778300001'
pmid:
- '39501563'
file:
- access_level: open_access
checksum: a11feea4b1677df76b632eca04bfc1dd
content_type: application/pdf
creator: dernst
date_created: 2024-12-03T11:08:09Z
date_updated: 2024-12-03T11:08:09Z
file_id: '18612'
file_name: 2024_MolecularPlant_Kralova.pdf
file_size: 3308945
relation: main_file
success: 1
file_date_updated: 2024-12-03T11:08:09Z
fulldoi: https://doi.org/10.1016/j.molp.2024.11.001
has_accepted_license: '1'
intvolume: ' 17'
isi: 1
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 1850-1865
pmid: 1
publication: Molecular Plant
publication_identifier:
issn:
- 1674-2052
publication_status: published
publisher: Elsevier
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: yes
title: A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling
in Arabidopsis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 17
year: '2024'
...
---
OA_place: publisher
OA_type: hybrid
_id: '15381'
abstract:
- lang: eng
text: 'Cholecystokinin-expressing interneurons (CCKIs) are hypothesized to shape
pyramidal cell-firing patterns and regulate network oscillations and related network
state transitions. To directly probe their role in the CA1 region, we silenced
their activity using optogenetic and chemogenetic tools in mice. Opto-tagged CCKIs
revealed a heterogeneous population, and their optogenetic silencing triggered
wide disinhibitory network changes affecting both pyramidal cells and other interneurons.
CCKI silencing enhanced pyramidal cell burst firing and altered the temporal coding
of place cells: theta phase precession was disrupted, whereas sequence reactivation
was enhanced. Chemogenetic CCKI silencing did not alter the acquisition of spatial
reference memories on the Morris water maze but enhanced the recall of contextual
fear memories and enabled selective recall when similar environments were tested.
This work suggests the key involvement of CCKIs in the control of place-cell temporal
coding and the formation of contextual memories.'
acknowledged_ssus:
- _id: M-Shop
- _id: Bio
- _id: LifeSc
- _id: PreCl
acknowledgement: We thank the kind donations from Andrea Varro, Brian Sauer, Edward
Boyden, and Peter Jonas. We thank Jago Wallenschus, Kerstin Kronenbitter, and Didier
Gremelle for outstanding technical support; Laura Bollepalli for initial viral targeting
experiments; Cihan Önal for initial electrophysiology experiments; Yoav Ben-Simon
for histological advice; and Anton Nikitenko for contributing to the analysis. We
acknowledge support from the Miba Machine Shop, Bioimaging-, Life Science- and Pre-Clinical
Facilities at ISTA. This work was supported by the Austrian Science Fund (FWF I3713
to J.C. as part of the FOR 2143 research consortium), the Deutsche Forschungsgemeinschaft
(DFG) (WU 503/2-2 to P.W.), and the Medical Research Council, United Kingdom (grant
G1100546/2 to P.W.).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Dámaris K
full_name: Rangel Guerrero, Dámaris K
id: 4871BCE6-F248-11E8-B48F-1D18A9856A87
last_name: Rangel Guerrero
orcid: 0000-0002-8602-4374
- first_name: Kira
full_name: Balueva, Kira
last_name: Balueva
- first_name: Uladzislau
full_name: Barayeu, Uladzislau
id: b515be12-ec90-11ea-b966-d0b5e15613d2
last_name: Barayeu
- first_name: Peter
full_name: Baracskay, Peter
id: 361CC00E-F248-11E8-B48F-1D18A9856A87
last_name: Baracskay
- first_name: Igor
full_name: Gridchyn, Igor
id: 4B60654C-F248-11E8-B48F-1D18A9856A87
last_name: Gridchyn
orcid: 0000-0002-1807-1929
- first_name: Michele
full_name: Nardin, Michele
id: 30BD0376-F248-11E8-B48F-1D18A9856A87
last_name: Nardin
orcid: 0000-0001-8849-6570
- first_name: Chiara N
full_name: Roth, Chiara N
id: 37BB4FB6-F248-11E8-B48F-1D18A9856A87
last_name: Roth
- first_name: Peer
full_name: Wulff, Peer
last_name: Wulff
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
citation:
ama: Rangel Guerrero DK, Balueva K, Barayeu U, et al. Hippocampal cholecystokinin-expressing
interneurons regulate temporal coding and contextual learning. Neuron.
2024;112(12):2045-2061.e10. doi:10.1016/j.neuron.2024.03.019
apa: Rangel Guerrero, D. K., Balueva, K., Barayeu, U., Baracskay, P., Gridchyn,
I., Nardin, M., … Csicsvari, J. L. (2024). Hippocampal cholecystokinin-expressing
interneurons regulate temporal coding and contextual learning. Neuron.
Cell Press. https://doi.org/10.1016/j.neuron.2024.03.019
chicago: Rangel Guerrero, Dámaris K, Kira Balueva, Uladzislau Barayeu, Peter Baracskay,
Igor Gridchyn, Michele Nardin, Chiara N Roth, Peer Wulff, and Jozsef L Csicsvari.
“Hippocampal Cholecystokinin-Expressing Interneurons Regulate Temporal Coding
and Contextual Learning.” Neuron. Cell Press, 2024. https://doi.org/10.1016/j.neuron.2024.03.019.
ieee: D. K. Rangel Guerrero et al., “Hippocampal cholecystokinin-expressing
interneurons regulate temporal coding and contextual learning,” Neuron,
vol. 112, no. 12. Cell Press, p. 2045–2061.e10, 2024.
ista: Rangel Guerrero DK, Balueva K, Barayeu U, Baracskay P, Gridchyn I, Nardin
M, Roth CN, Wulff P, Csicsvari JL. 2024. Hippocampal cholecystokinin-expressing
interneurons regulate temporal coding and contextual learning. Neuron. 112(12),
2045–2061.e10.
mla: Rangel Guerrero, Dámaris K., et al. “Hippocampal Cholecystokinin-Expressing
Interneurons Regulate Temporal Coding and Contextual Learning.” Neuron,
vol. 112, no. 12, Cell Press, 2024, p. 2045–2061.e10, doi:10.1016/j.neuron.2024.03.019.
short: D.K. Rangel Guerrero, K. Balueva, U. Barayeu, P. Baracskay, I. Gridchyn,
M. Nardin, C.N. Roth, P. Wulff, J.L. Csicsvari, Neuron 112 (2024) 2045–2061.e10.
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: "Data reported in this paper can be shared by the lead
contact upon justified request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny
additional information required to reanalyze the data reported in this work is available
from the corresponding authors upon request."
date_created: 2024-05-12T22:01:03Z
date_published: 2024-06-19T00:00:00Z
date_updated: 2026-10-15T08:38:29Z
day: '19'
ddc:
- '570'
department:
- _id: JoCs
doi: 10.1016/j.neuron.2024.03.019
external_id:
isi:
- '001300571400001'
pmid:
- '38636524'
file:
- access_level: open_access
checksum: de5b18ff293d42bd90e83a193e889844
content_type: application/pdf
creator: dernst
date_created: 2025-01-09T09:15:31Z
date_updated: 2025-01-09T09:15:31Z
file_id: '18798'
file_name: 2024_Neuron_RangelGuerrero.pdf
file_size: 9149079
relation: main_file
success: 1
file_date_updated: 2025-01-09T09:15:31Z
fulldoi: https://doi.org/10.1016/j.neuron.2024.03.019
has_accepted_license: '1'
intvolume: ' 112'
isi: 1
issue: '12'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 2045-2061.e10
pmid: 1
project:
- _id: 2654F984-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 3713-B27
name: Interneuro plasticity during spatial learning
publication: Neuron
publication_identifier:
eissn:
- 1097-4199
issn:
- 0896-6273
publication_status: published
publisher: Cell Press
quality_controlled: '1'
researchdata_availability: upon request
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Hippocampal cholecystokinin-expressing interneurons regulate temporal coding
and contextual learning
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 112
year: '2024'
...
---
_id: '15374'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) is an essential process of cargo uptake
operating in all eukaryotes. In animals and yeast, BAR-SH3 domain proteins, endophilins
and amphiphysins, function at the conclusion of CME to recruit factors for vesicle
scission and uncoating. Arabidopsis thaliana contains the BAR-SH3 domain proteins
SH3P1–SH3P3, but their role is poorly understood. Here, we identify SH3Ps as functional
homologs of endophilin/amphiphysin. SH3P1–SH3P3 bind to discrete foci at the plasma
membrane (PM), and SH3P2 recruits late to a subset of clathrin-coated pits. The
SH3P2 PM recruitment pattern is nearly identical to its interactor, a putative
uncoating factor, AUXILIN-LIKE1. Notably, SH3P1–SH3P3 are required for most of
AUXILIN-LIKE1 recruitment to the PM. This indicates a plant-specific modification
of CME, where BAR-SH3 proteins recruit auxilin-like uncoating factors rather than
the uncoating phosphatases, synaptojanins. SH3P1–SH3P3 act redundantly in overall
CME with the plant-specific endocytic adaptor TPLATE complex but not due to an
SH3 domain in its TASH3 subunit.
acknowledgement: 'The authors wish to acknowledge Dr. Daniel van Damme for mRuby3/pDONRP2rP3
and Prof. Qi-Jun Chen for sharing plasmids used for CRISPR-Cas9 mutagenesis. This
work was supported by the Austrian Science Fund (FWF): I 3630-B25.'
article_number: '114195'
article_processing_charge: Yes
article_type: original
author:
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Marek
full_name: Randuch, Marek
id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
last_name: Randuch
- first_name: Ivana
full_name: Matijevic, Ivana
id: 83c17ce3-15b2-11ec-abd3-f486545870bd
last_name: Matijevic
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Adamowski M, Randuch M, Matijevic I, Narasimhan M, Friml J. SH3Ps recruit auxilin-like
vesicle uncoating factors for clathrin-mediated endocytosis. Cell Reports.
2024;43(5). doi:10.1016/j.celrep.2024.114195
apa: Adamowski, M., Randuch, M., Matijevic, I., Narasimhan, M., & Friml, J.
(2024). SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated
endocytosis. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2024.114195
chicago: Adamowski, Maciek, Marek Randuch, Ivana Matijevic, Madhumitha Narasimhan,
and Jiří Friml. “SH3Ps Recruit Auxilin-like Vesicle Uncoating Factors for Clathrin-Mediated
Endocytosis.” Cell Reports. Cell Press, 2024. https://doi.org/10.1016/j.celrep.2024.114195.
ieee: M. Adamowski, M. Randuch, I. Matijevic, M. Narasimhan, and J. Friml, “SH3Ps
recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis,”
Cell Reports, vol. 43, no. 5. Cell Press, 2024.
ista: Adamowski M, Randuch M, Matijevic I, Narasimhan M, Friml J. 2024. SH3Ps recruit
auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis. Cell
Reports. 43(5), 114195.
mla: Adamowski, Maciek, et al. “SH3Ps Recruit Auxilin-like Vesicle Uncoating Factors
for Clathrin-Mediated Endocytosis.” Cell Reports, vol. 43, no. 5, 114195,
Cell Press, 2024, doi:10.1016/j.celrep.2024.114195.
short: M. Adamowski, M. Randuch, I. Matijevic, M. Narasimhan, J. Friml, Cell Reports
43 (2024).
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: "All data reported in this paper will be shared by the
lead contact upon request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny
additional information required to reanalyze the data reported in this work paper
is available from the lead contact upon request."
date_created: 2024-05-12T22:01:01Z
date_published: 2024-05-28T00:00:00Z
date_updated: 2026-10-15T08:34:31Z
day: '28'
ddc:
- '580'
department:
- _id: JiFr
- _id: MaLo
doi: 10.1016/j.celrep.2024.114195
external_id:
isi:
- '001240362800001'
pmid:
- '38717900'
file:
- access_level: open_access
checksum: a06bb85be4fc765c51554d27ee2da802
content_type: application/pdf
creator: dernst
date_created: 2024-05-13T12:11:22Z
date_updated: 2024-05-13T12:11:22Z
file_id: '15387'
file_name: 2024_CellReports_Adamowski.pdf
file_size: 5698598
relation: main_file
success: 1
file_date_updated: 2024-05-13T12:11:22Z
fulldoi: https://doi.org/10.1016/j.celrep.2024.114195
has_accepted_license: '1'
intvolume: ' 43'
isi: 1
issue: '5'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Cell Reports
publication_identifier:
eissn:
- 2211-1247
publication_status: published
publisher: Cell Press
quality_controlled: '1'
researchdata_availability: upon request
scopus_import: '1'
status: public
supplementarymaterial: yes
title: SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated
endocytosis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 43
year: '2024'
...