[{"date_created":"2019-12-04T16:09:29Z","year":"2020","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","volume":528,"date_updated":"2023-08-17T14:06:50Z","ddc":["571","599"],"date_published":"2020-04-01T00:00:00Z","doi":"10.1002/cne.24792","abstract":[{"lang":"eng","text":"In the cerebellum, GluD2 is exclusively expressed in Purkinje cells, where it regulates synapse formation and regeneration, synaptic plasticity, and motor learning. Delayed cognitive development in humans with GluD2 gene mutations suggests extracerebellar functions of GluD2. However, extracerebellar expression of GluD2 and its relationship with that of GluD1 are poorly understood. GluD2 mRNA and protein were widely detected, with relatively high levels observed in the olfactory glomerular layer, medial prefrontal cortex, cingulate cortex, retrosplenial granular cortex, olfactory tubercle, subiculum, striatum, lateral septum, anterodorsal thalamic nucleus, and arcuate hypothalamic nucleus. These regions were also enriched for GluD1, and many individual neurons coexpressed the two GluDs. In the retrosplenial granular cortex, GluD1 and GluD2 were selectively expressed at PSD‐95‐expressing glutamatergic synapses, and their coexpression on the same synapses was shown by SDS‐digested freeze‐fracture replica labeling. Biochemically, GluD1 and GluD2 formed coimmunoprecipitable complex formation in HEK293T cells and in the cerebral cortex and hippocampus. We further estimated the relative protein amount by quantitative immunoblotting using GluA2/GluD2 and GluA2/GluD1 chimeric proteins as standards for titration of GluD1 and GluD2 antibodies. Intriguingly, the relative amount of GluD2 was almost comparable to that of GluD1 in the postsynaptic density fraction prepared from the cerebral cortex and hippocampus. In contrast, GluD2 was overwhelmingly predominant in the cerebellum. Thus, we have determined the relative extracerebellar expression of GluD1 and GluD2 at regional, neuronal, and synaptic levels. These data provide a molecular–anatomical basis for possible competitive and cooperative interactions of GluD family members at synapses in various brain regions."}],"external_id":{"isi":["000496410200001"],"pmid":["31625608"]},"department":[{"_id":"RySh"}],"publication_status":"published","publication":"Journal of Comparative Neurology","intvolume":"       528","publisher":"Wiley","publication_identifier":{"eissn":["1096-9861"],"issn":["0021-9967"]},"quality_controlled":"1","month":"04","page":"1003-1027","status":"public","type":"journal_article","day":"01","author":[{"full_name":"Nakamoto, Chihiro","last_name":"Nakamoto","first_name":"Chihiro"},{"last_name":"Konno","full_name":"Konno, Kohtarou","first_name":"Kohtarou"},{"first_name":"Taisuke","last_name":"Miyazaki","full_name":"Miyazaki, Taisuke"},{"first_name":"Ena","last_name":"Nakatsukasa","full_name":"Nakatsukasa, Ena"},{"last_name":"Natsume","full_name":"Natsume, Rie","first_name":"Rie"},{"last_name":"Abe","full_name":"Abe, Manabu","first_name":"Manabu"},{"first_name":"Meiko","full_name":"Kawamura, Meiko","last_name":"Kawamura"},{"full_name":"Fukazawa, Yugo","last_name":"Fukazawa","first_name":"Yugo"},{"last_name":"Shigemoto","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi"},{"full_name":"Yamasaki, Miwako","last_name":"Yamasaki","first_name":"Miwako"},{"first_name":"Kenji","last_name":"Sakimura","full_name":"Sakimura, Kenji"},{"first_name":"Masahiko","last_name":"Watanabe","full_name":"Watanabe, Masahiko"}],"language":[{"iso":"eng"}],"issue":"6","_id":"7148","article_type":"original","article_processing_charge":"No","isi":1,"oa_version":"None","acknowledgement":"This study was supported by Grants-in-Aid for Scientific Research to K.K. (18K06813), Y.M. (17K08503, 17H0631319), and K.S. (16H04650) and a grant for Scientific Research on Innovative Areas to K.S (16H06276) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT). We thank K. Akashi, I. Watanabe-Iida, Y. Suzuki, and H. Azechi for technical assistance and advice, and H. Uchida for valuable discussions. We thank E. Kushiya,I. Yabe, C. Ohori, Y. Mochizuki, Y. Ishikawa, and N. Ishimoto for technical assistance in generating GluD1-KO mice.","scopus_import":"1","title":"Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain","has_accepted_license":"1","citation":{"mla":"Nakamoto, Chihiro, et al. “Expression Mapping, Quantification, and Complex Formation of GluD1 and GluD2 Glutamate Receptors in Adult Mouse Brain.” <i>Journal of Comparative Neurology</i>, vol. 528, no. 6, Wiley, 2020, pp. 1003–27, doi:<a href=\"https://doi.org/10.1002/cne.24792\">10.1002/cne.24792</a>.","chicago":"Nakamoto, Chihiro, Kohtarou Konno, Taisuke Miyazaki, Ena Nakatsukasa, Rie Natsume, Manabu Abe, Meiko Kawamura, et al. “Expression Mapping, Quantification, and Complex Formation of GluD1 and GluD2 Glutamate Receptors in Adult Mouse Brain.” <i>Journal of Comparative Neurology</i>. Wiley, 2020. <a href=\"https://doi.org/10.1002/cne.24792\">https://doi.org/10.1002/cne.24792</a>.","short":"C. Nakamoto, K. Konno, T. Miyazaki, E. Nakatsukasa, R. Natsume, M. Abe, M. Kawamura, Y. Fukazawa, R. Shigemoto, M. Yamasaki, K. Sakimura, M. Watanabe, Journal of Comparative Neurology 528 (2020) 1003–1027.","ieee":"C. Nakamoto <i>et al.</i>, “Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain,” <i>Journal of Comparative Neurology</i>, vol. 528, no. 6. Wiley, pp. 1003–1027, 2020.","ama":"Nakamoto C, Konno K, Miyazaki T, et al. Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain. <i>Journal of Comparative Neurology</i>. 2020;528(6):1003-1027. doi:<a href=\"https://doi.org/10.1002/cne.24792\">10.1002/cne.24792</a>","ista":"Nakamoto C, Konno K, Miyazaki T, Nakatsukasa E, Natsume R, Abe M, Kawamura M, Fukazawa Y, Shigemoto R, Yamasaki M, Sakimura K, Watanabe M. 2020. Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain. Journal of Comparative Neurology. 528(6), 1003–1027.","apa":"Nakamoto, C., Konno, K., Miyazaki, T., Nakatsukasa, E., Natsume, R., Abe, M., … Watanabe, M. (2020). Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain. <i>Journal of Comparative Neurology</i>. Wiley. <a href=\"https://doi.org/10.1002/cne.24792\">https://doi.org/10.1002/cne.24792</a>"},"pmid":1},{"oa":1,"language":[{"iso":"eng"}],"author":[{"last_name":"Martín-Belmonte","full_name":"Martín-Belmonte, Alejandro","first_name":"Alejandro"},{"first_name":"Carolina","full_name":"Aguado, Carolina","last_name":"Aguado"},{"first_name":"Rocío","full_name":"Alfaro-Ruíz, Rocío","last_name":"Alfaro-Ruíz"},{"first_name":"Ana Esther","last_name":"Moreno-Martínez","full_name":"Moreno-Martínez, Ana Esther"},{"first_name":"Luis","last_name":"De La Ossa","full_name":"De La Ossa, Luis"},{"full_name":"Martínez-Hernández, José","last_name":"Martínez-Hernández","first_name":"José"},{"first_name":"Alain","last_name":"Buisson","full_name":"Buisson, Alain"},{"last_name":"Früh","full_name":"Früh, Simon","first_name":"Simon"},{"full_name":"Bettler, Bernhard","last_name":"Bettler","first_name":"Bernhard"},{"first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444"},{"first_name":"Yugo","last_name":"Fukazawa","full_name":"Fukazawa, Yugo"},{"first_name":"Rafael","last_name":"Luján","full_name":"Luján, Rafael"}],"article_type":"original","article_processing_charge":"No","issue":"3","_id":"7207","month":"05","quality_controlled":"1","publication_identifier":{"issn":["1015-6305"],"eissn":["1750-3639"]},"publisher":"Wiley","day":"01","type":"journal_article","status":"public","page":"554-575","citation":{"chicago":"Martín-Belmonte, Alejandro, Carolina Aguado, Rocío Alfaro-Ruíz, Ana Esther Moreno-Martínez, Luis De La Ossa, José Martínez-Hernández, Alain Buisson, et al. “Reduction in the Neuronal Surface of Post and Presynaptic GABA&#62;B&#60; Receptors in the Hippocampus in a Mouse Model of Alzheimer’s Disease.” <i>Brain Pathology</i>. Wiley, 2020. <a href=\"https://doi.org/10.1111/bpa.12802\">https://doi.org/10.1111/bpa.12802</a>.","mla":"Martín-Belmonte, Alejandro, et al. “Reduction in the Neuronal Surface of Post and Presynaptic GABA&#62;B&#60; Receptors in the Hippocampus in a Mouse Model of Alzheimer’s Disease.” <i>Brain Pathology</i>, vol. 30, no. 3, Wiley, 2020, pp. 554–75, doi:<a href=\"https://doi.org/10.1111/bpa.12802\">10.1111/bpa.12802</a>.","ama":"Martín-Belmonte A, Aguado C, Alfaro-Ruíz R, et al. Reduction in the neuronal surface of post and presynaptic GABA&#62;B&#60; receptors in the hippocampus in a mouse model of Alzheimer’s disease. <i>Brain Pathology</i>. 2020;30(3):554-575. doi:<a href=\"https://doi.org/10.1111/bpa.12802\">10.1111/bpa.12802</a>","short":"A. Martín-Belmonte, C. Aguado, R. Alfaro-Ruíz, A.E. Moreno-Martínez, L. De La Ossa, J. Martínez-Hernández, A. Buisson, S. Früh, B. Bettler, R. Shigemoto, Y. Fukazawa, R. Luján, Brain Pathology 30 (2020) 554–575.","ieee":"A. Martín-Belmonte <i>et al.</i>, “Reduction in the neuronal surface of post and presynaptic GABA&#62;B&#60; receptors in the hippocampus in a mouse model of Alzheimer’s disease,” <i>Brain Pathology</i>, vol. 30, no. 3. Wiley, pp. 554–575, 2020.","ista":"Martín-Belmonte A, Aguado C, Alfaro-Ruíz R, Moreno-Martínez AE, De La Ossa L, Martínez-Hernández J, Buisson A, Früh S, Bettler B, Shigemoto R, Fukazawa Y, Luján R. 2020. Reduction in the neuronal surface of post and presynaptic GABA&#62;B&#60; receptors in the hippocampus in a mouse model of Alzheimer’s disease. Brain Pathology. 30(3), 554–575.","apa":"Martín-Belmonte, A., Aguado, C., Alfaro-Ruíz, R., Moreno-Martínez, A. E., De La Ossa, L., Martínez-Hernández, J., … Luján, R. (2020). Reduction in the neuronal surface of post and presynaptic GABA&#62;B&#60; receptors in the hippocampus in a mouse model of Alzheimer’s disease. <i>Brain Pathology</i>. Wiley. <a href=\"https://doi.org/10.1111/bpa.12802\">https://doi.org/10.1111/bpa.12802</a>"},"has_accepted_license":"1","pmid":1,"scopus_import":"1","oa_version":"Published Version","isi":1,"title":"Reduction in the neuronal surface of post and presynaptic GABA>B< receptors in the hippocampus in a mouse model of Alzheimer's disease","date_updated":"2025-07-10T11:54:22Z","volume":30,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"date_published":"2020-05-01T00:00:00Z","project":[{"call_identifier":"H2020","_id":"25CBA828-B435-11E9-9278-68D0E5697425","grant_number":"720270","name":"Human Brain Project Specific Grant Agreement 1"},{"grant_number":"785907","_id":"26436750-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Human Brain Project Specific Grant Agreement 2"}],"ddc":["570"],"year":"2020","date_created":"2019-12-22T23:00:43Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Brain Pathology","intvolume":"        30","file":[{"access_level":"open_access","file_size":4220935,"file_name":"2020_BrainPathology_MartinBelmonte.pdf","date_updated":"2020-09-22T09:47:19Z","creator":"dernst","date_created":"2020-09-22T09:47:19Z","success":1,"content_type":"application/pdf","file_id":"8554","checksum":"549cc1b18f638a21d17a939ba5563fa9","relation":"main_file"}],"file_date_updated":"2020-09-22T09:47:19Z","abstract":[{"lang":"eng","text":"The hippocampus plays key roles in learning and memory and is a main target of Alzheimer's disease (AD), which causes progressive memory impairments. Despite numerous investigations about the processes required for the normal hippocampal functions, the neurotransmitter receptors involved in the synaptic deficits by which AD disables the hippocampus are not yet characterized. By combining histoblots, western blots, immunohistochemistry and high‐resolution immunoelectron microscopic methods for GABAB receptors, this study provides a quantitative description of the expression and the subcellular localization of GABAB1 in the hippocampus in a mouse model of AD at 1, 6 and 12 months of age. Western blots and histoblots showed that the total amount of protein and the laminar expression pattern of GABAB1 were similar in APP/PS1 mice and in age‐matched wild‐type mice. In contrast, immunoelectron microscopic techniques showed that the subcellular localization of GABAB1 subunit did not change significantly in APP/PS1 mice at 1 month of age, was significantly reduced in the stratum lacunosum‐moleculare of CA1 pyramidal cells at 6 months of age and significantly reduced at the membrane surface of CA1 pyramidal cells at 12 months of age. This reduction of plasma membrane GABAB1 was paralleled by a significant increase of the subunit at the intracellular sites. We further observed a decrease of membrane‐targeted GABAB receptors in axon terminals contacting CA1 pyramidal cells. Our data demonstrate compartment‐ and age‐dependent reduction of plasma membrane‐targeted GABAB receptors in the CA1 region of the hippocampus, suggesting that this decrease might be enough to alter the GABAB‐mediated synaptic transmission taking place in AD."}],"ec_funded":1,"doi":"10.1111/bpa.12802","publication_status":"published","department":[{"_id":"RySh"}],"external_id":{"pmid":["31729777"],"isi":["000502270900001"]}},{"language":[{"iso":"eng"}],"oa":1,"author":[{"first_name":"Lashmi","full_name":"Piriya Ananda Babu, Lashmi","last_name":"Piriya Ananda Babu"},{"full_name":"Wang, Han Ying","last_name":"Wang","first_name":"Han Ying"},{"first_name":"Kohgaku","orcid":"0000-0002-6170-2546","full_name":"Eguchi, Kohgaku","last_name":"Eguchi","id":"2B7846DC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Guillaud, Laurent","last_name":"Guillaud","first_name":"Laurent"},{"full_name":"Takahashi, Tomoyuki","last_name":"Takahashi","first_name":"Tomoyuki"}],"article_type":"original","article_processing_charge":"No","_id":"7339","issue":"1","quality_controlled":"1","month":"01","publication_identifier":{"issn":["0270-6474"],"eissn":["1529-2401"]},"publisher":"Society for Neuroscience","type":"journal_article","day":"02","status":"public","page":"131-142","citation":{"mla":"Piriya Ananda Babu, Lashmi, et al. “Microtubule and Actin Differentially Regulate Synaptic Vesicle Cycling to Maintain High-Frequency Neurotransmission.” <i>Journal of Neuroscience</i>, vol. 40, no. 1, Society for Neuroscience, 2020, pp. 131–42, doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1571-19.2019\">10.1523/JNEUROSCI.1571-19.2019</a>.","chicago":"Piriya Ananda Babu, Lashmi, Han Ying Wang, Kohgaku Eguchi, Laurent Guillaud, and Tomoyuki Takahashi. “Microtubule and Actin Differentially Regulate Synaptic Vesicle Cycling to Maintain High-Frequency Neurotransmission.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 2020. <a href=\"https://doi.org/10.1523/JNEUROSCI.1571-19.2019\">https://doi.org/10.1523/JNEUROSCI.1571-19.2019</a>.","short":"L. Piriya Ananda Babu, H.Y. Wang, K. Eguchi, L. Guillaud, T. Takahashi, Journal of Neuroscience 40 (2020) 131–142.","ama":"Piriya Ananda Babu L, Wang HY, Eguchi K, Guillaud L, Takahashi T. Microtubule and actin differentially regulate synaptic vesicle cycling to maintain high-frequency neurotransmission. <i>Journal of neuroscience</i>. 2020;40(1):131-142. doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1571-19.2019\">10.1523/JNEUROSCI.1571-19.2019</a>","ieee":"L. Piriya Ananda Babu, H. Y. Wang, K. Eguchi, L. Guillaud, and T. Takahashi, “Microtubule and actin differentially regulate synaptic vesicle cycling to maintain high-frequency neurotransmission,” <i>Journal of neuroscience</i>, vol. 40, no. 1. Society for Neuroscience, pp. 131–142, 2020.","apa":"Piriya Ananda Babu, L., Wang, H. Y., Eguchi, K., Guillaud, L., &#38; Takahashi, T. (2020). Microtubule and actin differentially regulate synaptic vesicle cycling to maintain high-frequency neurotransmission. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href=\"https://doi.org/10.1523/JNEUROSCI.1571-19.2019\">https://doi.org/10.1523/JNEUROSCI.1571-19.2019</a>","ista":"Piriya Ananda Babu L, Wang HY, Eguchi K, Guillaud L, Takahashi T. 2020. Microtubule and actin differentially regulate synaptic vesicle cycling to maintain high-frequency neurotransmission. Journal of neuroscience. 40(1), 131–142."},"has_accepted_license":"1","pmid":1,"scopus_import":"1","oa_version":"Published Version","isi":1,"title":"Microtubule and actin differentially regulate synaptic vesicle cycling to maintain high-frequency neurotransmission","volume":40,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"date_updated":"2026-04-16T08:27:29Z","ddc":["570"],"date_published":"2020-01-02T00:00:00Z","year":"2020","date_created":"2020-01-19T23:00:38Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","file":[{"content_type":"application/pdf","date_created":"2020-01-20T14:44:10Z","relation":"main_file","file_id":"7345","checksum":"92f5e8a47f454fc131fb94cd7f106e60","file_size":4460781,"file_name":"2020_JourNeuroscience_Piriya.pdf","date_updated":"2020-07-14T12:47:56Z","access_level":"open_access","creator":"dernst"}],"publication":"Journal of neuroscience","intvolume":"        40","file_date_updated":"2020-07-14T12:47:56Z","abstract":[{"text":"Cytoskeletal filaments such as microtubules (MTs) and filamentous actin (F-actin) dynamically support cell structure and functions. In central presynaptic terminals, F-actin is expressed along the release edge and reportedly plays diverse functional roles, but whether axonal MTs extend deep into terminals and play any physiological role remains controversial. At the calyx of Held in rats of either sex, confocal and high-resolution microscopy revealed that MTs enter deep into presynaptic terminal swellings and partially colocalize with a subset of synaptic vesicles (SVs). Electrophysiological analysis demonstrated that depolymerization of MTs specifically prolonged the slow-recovery time component of EPSCs from short-term depression induced by a train of high-frequency stimulation, whereas depolymerization of F-actin specifically prolonged the fast-recovery component. In simultaneous presynaptic and postsynaptic action potential recordings, depolymerization of MTs or F-actin significantly impaired the fidelity of high-frequency neurotransmission. We conclude that MTs and F-actin differentially contribute to slow and fast SV replenishment, thereby maintaining high-frequency neurotransmission.","lang":"eng"}],"doi":"10.1523/JNEUROSCI.1571-19.2019","publication_status":"published","external_id":{"pmid":["31767677"],"isi":["000505167600013"]},"department":[{"_id":"RySh"}]},{"abstract":[{"lang":"eng","text":"The medial habenula (MHb) is an evolutionary conserved epithalamic structure important for the modulation of emotional memory. It is involved in regulation of anxiety, compulsive behavior, addiction (nicotinic and opioid), sexual and feeding behavior. MHb receives inputs from septal regions and projects exclusively to the interpeduncular nucleus (IPN). Distinct sub-regions of the septum project to different subnuclei of MHb: the bed nucleus of anterior commissure projects to dorsal MHb and the triangular septum projects to ventral MHb. Furthermore, the dorsal and ventral MHb project to the lateral and rostral/central IPN, respectively. Importantly, these projections have unique features of prominent co-release of different neurotransmitters and requirement of a peculiar type of calcium channel for release. In general, synaptic neurotransmission requires an activity-dependent influx of Ca2+ into the presynaptic terminal through voltage-gated calcium channels. The calcium channel family most commonly involved in neurotransmitter release comprises three members, P/Q-, N- and R-type with Cav2.1, Cav2.2 and Cav2.3 subunits, respectively. In contrast to most CNS synapses that mainly express Cav2.1 and/or Cav2.2, MHb terminals in the IPN exclusively express Cav2.3. In other parts of the brain, such as the hippocampus, Cav2.3 is mostly located to postsynaptic elements. This unusual presynaptic location of Cav2.3 in the MHb-IPN pathway implies unique mechanisms of glutamate release in this pathway. One potential example of such uniqueness is the facilitation of release by GABAB receptor (GBR) activation. Presynaptic GBRs usually inhibit the release of neurotransmitters by inhibiting presynaptic calcium channels. MHb shows the highest expression levels of GBR in the brain. GBRs comprise two subunits, GABAB1 (GB1) and GABAB2 (GB2), and are associated with auxiliary subunits, called potassium channel tetramerization domain containing proteins (KCTD) 8, 12, 12b and 16. Among these four subunits, KCTD12b is exclusively expressed in ventral MHb, and KCTD8 shows the strongest expression in the whole MHb among other brain regions, indicating that KCTD8 and KCTD12b may be involved in the unique mechanisms of neurotransmitter release mediated by Cav2.3 and regulated by GBRs in this pathway. \r\nIn the present study, we first verified that neurotransmission in both dorsal and ventral MHb-IPN pathways is mainly mediated by Cav2.3 using a selective blocker of R-type channels, SNX-482. We next found that baclofen, a GBR agonist, has facilitatory effects on release from ventral MHb terminal in rostral IPN, whereas it has inhibitory effects on release from dorsal MHb terminals in lateral IPN, indicating that KCTD12b expressed exclusively in ventral MHb may have a role in the facilitatory effects of GBR activation. In a heterologous expression system using HEK cells, we found that KCTD8 and KCTD12b but not KCTD12 directly bind with Cav2.3. Pre-embedding immunogold electron microscopy data show that Cav2.3 and KCTD12b are distributed most densely in presynaptic active zone in IPN with KCTD12b being present only in rostral/central but not lateral IPN, whereas GABAB, KCTD8 and KCTD12 are distributed most densely in perisynaptic sites with KCTD12 present more frequently in postsynaptic elements and only in rostral/central IPN. In freeze-fracture replica labelling, Cav2.3, KCTD8 and KCTD12b are co-localized with each other in the same active zone indicating that they may form complexes regulating vesicle release in rostral IPN. \r\nOn electrophysiological studies of wild type (WT) mice, we found that paired-pulse ratio in rostral IPN of KCTD12b knock-out (KO) mice is lower than those of WT and KCTD8 KO mice. Consistent with this finding, in mean variance analysis, release probability in rostral IPN of KCTD12b KO mice is higher than that of WT and KCTD8 KO mice. Although paired-pulse ratios are not different between WT and KCTD8 KO mice, the mean variance analysis revealed significantly lower release probability in rostral IPN of KCTD8 KO than WT mice. These results demonstrate bidirectional regulation of Cav2.3-mediated release by KCTD8 and KCTD12b without GBR activation in rostral IPN. Finally, we examined the baclofen effects in rostral IPN of KCTD8 and KCTD12b KO mice, and found the facilitation of release remained in both KO mice, indicating that the peculiar effects of the GBR activation in this pathway do not depend on the selective expression of these KCTD subunits in ventral MHb. However, we found that presynaptic potentiation of evoked EPSC amplitude by baclofen falls to baseline after washout faster in KCTD12b KO mice than WT, KCTD8 KO and KCTD8/12b double KO mice. This result indicates that KCTD12b is involved in sustained potentiation of vesicle release by GBR activation, whereas KCTD8 is involved in its termination in the absence of KCTD12b. Consistent with these functional findings, replica labelling revealed an increase in density of KCTD8, but not Cav2.3 or GBR at active zone in rostral IPN of KCTD12b KO mice compared with that of WT mice, suggesting that increased association of KCTD8 with Cav2.3 facilitates the release probability and termination of the GBR effect in the absence of KCTD12b.\r\nIn summary, our study provided new insights into the physiological roles of presynaptic Cav2.3, GBRs and their auxiliary subunits KCTDs at an evolutionary conserved neuronal circuit. Future studies will be required to identify the exact molecular mechanism underlying the GBR-mediated presynaptic potentiation on ventral MHb terminals. It remains to be determined whether the prominent presence of presynaptic KCTDs at active zone could exert similar neuromodulatory functions in different pathways of the brain.\r\n"}],"degree_awarded":"PhD","doi":"10.15479/AT:ISTA:7525","alternative_title":["ISTA Thesis"],"publication_status":"published","department":[{"_id":"RySh"}],"file":[{"title":"Localization and functional role of Cav2.3 in the medial habenula to interpeduncular nucleus pathway","relation":"main_file","checksum":"4589234fdb12b4ad72273b311723a7b4","file_id":"7538","content_type":"application/pdf","date_created":"2020-02-28T08:37:53Z","creator":"pbhandari","embargo":"2021-02-28","file_name":"Pradeep Bhandari Thesis.pdf","date_updated":"2021-03-01T23:30:04Z","file_size":9646346,"access_level":"open_access"},{"file_name":"Pradeep Bhandari Thesis.docx","date_updated":"2021-03-01T23:30:04Z","file_size":35252164,"access_level":"closed","embargo_to":"open_access","creator":"pbhandari","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2020-02-28T08:47:14Z","relation":"source_file","title":"Localization and functional role of Cav2.3 in the medial habenula to interpeduncular nucleus pathway","file_id":"7539","checksum":"aa79490553ca0a5c9b6fbcd152e93928"}],"file_date_updated":"2021-03-01T23:30:04Z","year":"2020","date_created":"2020-02-26T10:56:37Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-04-08T07:27:27Z","date_published":"2020-02-28T00:00:00Z","ddc":["570"],"oa_version":"Published Version","title":"Localization and functional role of Cav2.3 in the medial habenula to interpeduncular nucleus pathway","acknowledged_ssus":[{"_id":"EM-Fac"}],"citation":{"chicago":"Bhandari, Pradeep. “Localization and Functional Role of Cav2.3 in the Medial Habenula to Interpeduncular Nucleus Pathway.” Institute of Science and Technology Austria, 2020. <a href=\"https://doi.org/10.15479/AT:ISTA:7525\">https://doi.org/10.15479/AT:ISTA:7525</a>.","mla":"Bhandari, Pradeep. <i>Localization and Functional Role of Cav2.3 in the Medial Habenula to Interpeduncular Nucleus Pathway</i>. Institute of Science and Technology Austria, 2020, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:7525\">10.15479/AT:ISTA:7525</a>.","apa":"Bhandari, P. (2020). <i>Localization and functional role of Cav2.3 in the medial habenula to interpeduncular nucleus pathway</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:7525\">https://doi.org/10.15479/AT:ISTA:7525</a>","ista":"Bhandari P. 2020. Localization and functional role of Cav2.3 in the medial habenula to interpeduncular nucleus pathway. Institute of Science and Technology Austria.","short":"P. Bhandari, Localization and Functional Role of Cav2.3 in the Medial Habenula to Interpeduncular Nucleus Pathway, Institute of Science and Technology Austria, 2020.","ama":"Bhandari P. Localization and functional role of Cav2.3 in the medial habenula to interpeduncular nucleus pathway. 2020. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:7525\">10.15479/AT:ISTA:7525</a>","ieee":"P. Bhandari, “Localization and functional role of Cav2.3 in the medial habenula to interpeduncular nucleus pathway,” Institute of Science and Technology Austria, 2020."},"has_accepted_license":"1","month":"02","publication_identifier":{"issn":["2663-337X"]},"publisher":"Institute of Science and Technology Austria","day":"28","type":"dissertation","OA_place":"publisher","page":"79","status":"public","language":[{"iso":"eng"}],"oa":1,"author":[{"first_name":"Pradeep","id":"45EDD1BC-F248-11E8-B48F-1D18A9856A87","last_name":"Bhandari","full_name":"Bhandari, Pradeep","orcid":"0000-0003-0863-4481"}],"supervisor":[{"first_name":"Ryuichi","last_name":"Shigemoto","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"No","_id":"7525","keyword":["Cav2.3","medial habenula (MHb)","interpeduncular nucleus (IPN)"],"corr_author":"1"},{"article_processing_charge":"No","article_type":"original","corr_author":"1","_id":"8532","issue":"18","oa":1,"language":[{"iso":"eng"}],"author":[{"last_name":"Kleindienst","full_name":"Kleindienst, David","id":"42E121A4-F248-11E8-B48F-1D18A9856A87","first_name":"David"},{"full_name":"Montanaro-Punzengruber, Jacqueline-Claire","last_name":"Montanaro-Punzengruber","id":"3786AB44-F248-11E8-B48F-1D18A9856A87","first_name":"Jacqueline-Claire"},{"full_name":"Bhandari, Pradeep","orcid":"0000-0003-0863-4481","last_name":"Bhandari","id":"45EDD1BC-F248-11E8-B48F-1D18A9856A87","first_name":"Pradeep"},{"first_name":"Matthew J","id":"44B7CA5A-F248-11E8-B48F-1D18A9856A87","last_name":"Case","full_name":"Case, Matthew J"},{"first_name":"Yugo","last_name":"Fukazawa","full_name":"Fukazawa, Yugo"},{"last_name":"Shigemoto","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi"}],"type":"journal_article","day":"14","status":"public","month":"09","quality_controlled":"1","article_number":"6737","publisher":"MDPI","publication_identifier":{"issn":["1661-6596"],"eissn":["1422-0067"]},"citation":{"chicago":"Kleindienst, David, Jacqueline-Claire Montanaro-Punzengruber, Pradeep Bhandari, Matthew J Case, Yugo Fukazawa, and Ryuichi Shigemoto. “Deep Learning-Assisted High-Throughput Analysis of Freeze-Fracture Replica Images Applied to Glutamate Receptors and Calcium Channels at Hippocampal Synapses.” <i>International Journal of Molecular Sciences</i>. MDPI, 2020. <a href=\"https://doi.org/10.3390/ijms21186737\">https://doi.org/10.3390/ijms21186737</a>.","mla":"Kleindienst, David, et al. “Deep Learning-Assisted High-Throughput Analysis of Freeze-Fracture Replica Images Applied to Glutamate Receptors and Calcium Channels at Hippocampal Synapses.” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 18, 6737, MDPI, 2020, doi:<a href=\"https://doi.org/10.3390/ijms21186737\">10.3390/ijms21186737</a>.","ista":"Kleindienst D, Montanaro-Punzengruber J-C, Bhandari P, Case MJ, Fukazawa Y, Shigemoto R. 2020. Deep learning-assisted high-throughput analysis of freeze-fracture replica images applied to glutamate receptors and calcium channels at hippocampal synapses. International Journal of Molecular Sciences. 21(18), 6737.","apa":"Kleindienst, D., Montanaro-Punzengruber, J.-C., Bhandari, P., Case, M. J., Fukazawa, Y., &#38; Shigemoto, R. (2020). Deep learning-assisted high-throughput analysis of freeze-fracture replica images applied to glutamate receptors and calcium channels at hippocampal synapses. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms21186737\">https://doi.org/10.3390/ijms21186737</a>","short":"D. Kleindienst, J.-C. Montanaro-Punzengruber, P. Bhandari, M.J. Case, Y. Fukazawa, R. Shigemoto, International Journal of Molecular Sciences 21 (2020).","ama":"Kleindienst D, Montanaro-Punzengruber J-C, Bhandari P, Case MJ, Fukazawa Y, Shigemoto R. Deep learning-assisted high-throughput analysis of freeze-fracture replica images applied to glutamate receptors and calcium channels at hippocampal synapses. <i>International Journal of Molecular Sciences</i>. 2020;21(18). doi:<a href=\"https://doi.org/10.3390/ijms21186737\">10.3390/ijms21186737</a>","ieee":"D. Kleindienst, J.-C. Montanaro-Punzengruber, P. Bhandari, M. J. Case, Y. Fukazawa, and R. Shigemoto, “Deep learning-assisted high-throughput analysis of freeze-fracture replica images applied to glutamate receptors and calcium channels at hippocampal synapses,” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 18. MDPI, 2020."},"has_accepted_license":"1","title":"Deep learning-assisted high-throughput analysis of freeze-fracture replica images applied to glutamate receptors and calcium channels at hippocampal synapses","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"9562"}]},"oa_version":"Published Version","scopus_import":"1","acknowledgement":"This research was funded by Austrian Academy of Sciences, DOC fellowship to D.K., European Research\r\nCouncil Advanced Grant 694539 and European Union Human Brain Project (HBP) SGA2 785907 to R.S.\r\nWe acknowledge Elena Hollergschwandtner for technical support.","isi":1,"ddc":["570"],"project":[{"grant_number":"694539","_id":"25CA28EA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour"},{"_id":"25D32BC0-B435-11E9-9278-68D0E5697425","name":"Mechanism of formation and maintenance of input side-dependent asymmetry in the hippocampus"},{"name":"Human Brain Project Specific Grant Agreement 2","_id":"26436750-B435-11E9-9278-68D0E5697425","grant_number":"785907","call_identifier":"H2020"}],"date_published":"2020-09-14T00:00:00Z","volume":21,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"date_updated":"2026-08-09T22:31:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2020","date_created":"2020-09-20T22:01:35Z","file_date_updated":"2020-09-21T14:08:58Z","publication":"International Journal of Molecular Sciences","intvolume":"        21","file":[{"checksum":"2e4f62f3cfe945b7391fc3070e5a289f","file_id":"8551","relation":"main_file","date_created":"2020-09-21T14:08:58Z","content_type":"application/pdf","success":1,"creator":"dernst","access_level":"open_access","date_updated":"2020-09-21T14:08:58Z","file_size":5748456,"file_name":"2020_JournMolecSciences_Kleindienst.pdf"}],"publication_status":"published","external_id":{"isi":["000579945300001"]},"department":[{"_id":"RySh"}],"ec_funded":1,"abstract":[{"text":"The molecular anatomy of synapses defines their characteristics in transmission and plasticity. Precise measurements of the number and distribution of synaptic proteins are important for our understanding of synapse heterogeneity within and between brain regions. Freeze–fracture replica immunogold electron microscopy enables us to analyze them quantitatively on a two-dimensional membrane surface. Here, we introduce Darea software, which utilizes deep learning for analysis of replica images and demonstrate its usefulness for quick measurements of the pre- and postsynaptic areas, density and distribution of gold particles at synapses in a reproducible manner. We used Darea for comparing glutamate receptor and calcium channel distributions between hippocampal CA3-CA1 spine synapses on apical and basal dendrites, which differ in signaling pathways involved in synaptic plasticity. We found that apical synapses express a higher density of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors and a stronger increase of AMPA receptors with synaptic size, while basal synapses show a larger increase in N-methyl-D-aspartate (NMDA) receptors with size. Interestingly, AMPA and NMDA receptors are segregated within postsynaptic sites and negatively correlated in density among both apical and basal synapses. In the presynaptic sites, Cav2.1 voltage-gated calcium channels show similar densities in apical and basal synapses with distributions consistent with an exclusion zone model of calcium channel-release site topography.","lang":"eng"}],"doi":"10.3390/ijms21186737"},{"has_accepted_license":"1","citation":{"apa":"Zenmyo, N., Tokumaru, H., Uchinomiya, S., Fuchida, H., Tabata, S., Hamachi, I., … Ojida, A. (2019). Optimized reaction pair of the CysHis tag and Ni(II)-NTA probe for highly selective chemical labeling of membrane proteins. <i>Bulletin of the Chemical Society of Japan</i>. Bulletin of the Chemical Society of Japan. <a href=\"https://doi.org/10.1246/bcsj.20190034\">https://doi.org/10.1246/bcsj.20190034</a>","ista":"Zenmyo N, Tokumaru H, Uchinomiya S, Fuchida H, Tabata S, Hamachi I, Shigemoto R, Ojida A. 2019. Optimized reaction pair of the CysHis tag and Ni(II)-NTA probe for highly selective chemical labeling of membrane proteins. Bulletin of the Chemical Society of Japan. 92(5), 995–1000.","short":"N. Zenmyo, H. Tokumaru, S. Uchinomiya, H. Fuchida, S. Tabata, I. Hamachi, R. Shigemoto, A. Ojida, Bulletin of the Chemical Society of Japan 92 (2019) 995–1000.","ieee":"N. Zenmyo <i>et al.</i>, “Optimized reaction pair of the CysHis tag and Ni(II)-NTA probe for highly selective chemical labeling of membrane proteins,” <i>Bulletin of the Chemical Society of Japan</i>, vol. 92, no. 5. Bulletin of the Chemical Society of Japan, pp. 995–1000, 2019.","ama":"Zenmyo N, Tokumaru H, Uchinomiya S, et al. Optimized reaction pair of the CysHis tag and Ni(II)-NTA probe for highly selective chemical labeling of membrane proteins. <i>Bulletin of the Chemical Society of Japan</i>. 2019;92(5):995-1000. doi:<a href=\"https://doi.org/10.1246/bcsj.20190034\">10.1246/bcsj.20190034</a>","mla":"Zenmyo, Naoki, et al. “Optimized Reaction Pair of the CysHis Tag and Ni(II)-NTA Probe for Highly Selective Chemical Labeling of Membrane Proteins.” <i>Bulletin of the Chemical Society of Japan</i>, vol. 92, no. 5, Bulletin of the Chemical Society of Japan, 2019, pp. 995–1000, doi:<a href=\"https://doi.org/10.1246/bcsj.20190034\">10.1246/bcsj.20190034</a>.","chicago":"Zenmyo, Naoki, Hiroki Tokumaru, Shohei Uchinomiya, Hirokazu Fuchida, Shigekazu Tabata, Itaru Hamachi, Ryuichi Shigemoto, and Akio Ojida. “Optimized Reaction Pair of the CysHis Tag and Ni(II)-NTA Probe for Highly Selective Chemical Labeling of Membrane Proteins.” <i>Bulletin of the Chemical Society of Japan</i>. Bulletin of the Chemical Society of Japan, 2019. <a href=\"https://doi.org/10.1246/bcsj.20190034\">https://doi.org/10.1246/bcsj.20190034</a>."},"title":"Optimized reaction pair of the CysHis tag and Ni(II)-NTA probe for highly selective chemical labeling of membrane proteins","isi":1,"OA_type":"free access","acknowledgement":"his work was supported by the Grant-in-Aid for Scientific Research B (JSPS KAKENHI grant no. JP17H03090 to A. O.); the Scientific Research on Innovative Areas “Chemistry for Multimolecular Crowding Biosystems” (JSPS KAKENHI grant no. JP17H06349 to A. O.); and the European Union (European Research Council Advanced grant no. 694539 and Human Brain Project Ref. 720270 to R. S.). A. O. acknowledges the financial support of the Takeda Science Foundation.","scopus_import":"1","oa_version":"Published Version","_id":"6659","issue":"5","article_type":"original","article_processing_charge":"No","author":[{"last_name":"Zenmyo","full_name":"Zenmyo, Naoki","first_name":"Naoki"},{"full_name":"Tokumaru, Hiroki","last_name":"Tokumaru","first_name":"Hiroki"},{"full_name":"Uchinomiya, Shohei","last_name":"Uchinomiya","first_name":"Shohei"},{"full_name":"Fuchida, Hirokazu","last_name":"Fuchida","first_name":"Hirokazu"},{"first_name":"Shigekazu","last_name":"Tabata","full_name":"Tabata, Shigekazu","id":"4427179E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hamachi","full_name":"Hamachi, Itaru","first_name":"Itaru"},{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi"},{"first_name":"Akio","full_name":"Ojida, Akio","last_name":"Ojida"}],"language":[{"iso":"eng"}],"oa":1,"page":"995-1000","status":"public","type":"journal_article","day":"15","publication_identifier":{"issn":["0009-2673"]},"publisher":"Bulletin of the Chemical Society of Japan","month":"05","quality_controlled":"1","intvolume":"        92","publication":"Bulletin of the Chemical Society of Japan","external_id":{"isi":["000468101800007"]},"department":[{"_id":"RySh"}],"publication_status":"published","doi":"10.1246/bcsj.20190034","abstract":[{"text":"Chemical labeling of proteins with synthetic molecular probes offers the possibility to probe the functions of proteins of interest in living cells. However, the methods for covalently labeling targeted proteins using complementary peptide tag-probe pairs are still limited, irrespective of the versatility of such pairs in biological research. Herein, we report the new CysHis tag-Ni(II) probe pair for the specific covalent labeling of proteins. A broad-range evaluation of the reactivity profiles of the probe and the CysHis peptide tag afforded a tag-probe pair with an optimized and high labeling selectivity and reactivity. In particular, the labeling specificity of this pair was notably improved compared to the previously reported one. This pair was successfully utilized for the fluorescence imaging of membrane proteins on the surfaces of living cells, demonstrating its potential utility in biological research.","lang":"eng"}],"ec_funded":1,"project":[{"call_identifier":"H2020","grant_number":"694539","_id":"25CA28EA-B435-11E9-9278-68D0E5697425","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour"}],"ddc":["570"],"date_published":"2019-05-15T00:00:00Z","volume":92,"date_updated":"2025-09-10T10:37:21Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1246/bcsj.20190034"}],"date_created":"2019-07-21T21:59:16Z","year":"2019"},{"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_created":"2019-09-15T22:00:43Z","year":"2019","ddc":["570"],"date_published":"2019-09-09T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"volume":8,"date_updated":"2026-04-03T09:40:28Z","external_id":{"isi":["000485663900001"]},"department":[{"_id":"RySh"}],"publication_status":"published","doi":"10.7554/eLife.42766","abstract":[{"text":"Hyperpolarization-activated cyclic-nucleotide-gated (HCN) channels control electrical rhythmicity and excitability in the heart and brain, but the function of HCN channels at the subcellular level in axons remains poorly understood. Here, we show that the action potential conduction velocity in both myelinated and unmyelinated central axons can be bidirectionally modulated by a HCN channel blocker, cyclic adenosine monophosphate (cAMP), and neuromodulators. Recordings from mouse cerebellar mossy fiber boutons show that HCN channels ensure reliable high-frequency firing and are strongly modulated by cAMP (EC50 40 mM; estimated endogenous cAMP concentration 13 mM). In addition, immunogold-electron microscopy revealed HCN2 as the dominating subunit in cerebellar mossy fibers. Computational modeling indicated that HCN2 channels control conduction velocity primarily by altering the resting membrane potential\r\nand are associated with significant metabolic costs. These results suggest that the cAMP-HCN pathway provides neuromodulators with an opportunity to finely tune energy consumption and temporal delays across axons in the brain.","lang":"eng"}],"file_date_updated":"2020-07-14T12:47:42Z","publication":"eLife","file":[{"content_type":"application/pdf","date_created":"2019-09-16T13:14:33Z","relation":"main_file","checksum":"c350b7861ef0fb537cae8a3232aec016","file_id":"6880","date_updated":"2020-07-14T12:47:42Z","file_size":4008137,"file_name":"2019_eLife_Byczkowicz.pdf","access_level":"open_access","creator":"dernst"}],"intvolume":"         8","status":"public","type":"journal_article","day":"09","publication_identifier":{"eissn":["2050-084X"]},"publisher":"eLife Sciences Publications","article_number":"e42766","quality_controlled":"1","month":"09","_id":"6868","article_processing_charge":"No","article_type":"original","author":[{"last_name":"Byczkowicz","full_name":"Byczkowicz, Niklas","first_name":"Niklas"},{"full_name":"Eshra, Abdelmoneim","last_name":"Eshra","first_name":"Abdelmoneim"},{"first_name":"Jacqueline-Claire","full_name":"Montanaro-Punzengruber, Jacqueline-Claire","last_name":"Montanaro-Punzengruber","id":"3786AB44-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Andrea","last_name":"Trevisiol","full_name":"Trevisiol, Andrea"},{"first_name":"Johannes","last_name":"Hirrlinger","full_name":"Hirrlinger, Johannes"},{"full_name":"Kole, Maarten Hp","last_name":"Kole","first_name":"Maarten Hp"},{"first_name":"Ryuichi","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Stefan","full_name":"Hallermann, Stefan","last_name":"Hallermann"}],"oa":1,"language":[{"iso":"eng"}],"title":"HCN channel-mediated neuromodulation can control action potential velocity and fidelity in central axons","isi":1,"scopus_import":"1","oa_version":"Published Version","has_accepted_license":"1","citation":{"ama":"Byczkowicz N, Eshra A, Montanaro-Punzengruber J-C, et al. HCN channel-mediated neuromodulation can control action potential velocity and fidelity in central axons. <i>eLife</i>. 2019;8. doi:<a href=\"https://doi.org/10.7554/eLife.42766\">10.7554/eLife.42766</a>","ieee":"N. Byczkowicz <i>et al.</i>, “HCN channel-mediated neuromodulation can control action potential velocity and fidelity in central axons,” <i>eLife</i>, vol. 8. eLife Sciences Publications, 2019.","short":"N. Byczkowicz, A. Eshra, J.-C. Montanaro-Punzengruber, A. Trevisiol, J. Hirrlinger, M.H. Kole, R. Shigemoto, S. Hallermann, ELife 8 (2019).","ista":"Byczkowicz N, Eshra A, Montanaro-Punzengruber J-C, Trevisiol A, Hirrlinger J, Kole MH, Shigemoto R, Hallermann S. 2019. HCN channel-mediated neuromodulation can control action potential velocity and fidelity in central axons. eLife. 8, e42766.","apa":"Byczkowicz, N., Eshra, A., Montanaro-Punzengruber, J.-C., Trevisiol, A., Hirrlinger, J., Kole, M. H., … Hallermann, S. (2019). HCN channel-mediated neuromodulation can control action potential velocity and fidelity in central axons. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.42766\">https://doi.org/10.7554/eLife.42766</a>","chicago":"Byczkowicz, Niklas, Abdelmoneim Eshra, Jacqueline-Claire Montanaro-Punzengruber, Andrea Trevisiol, Johannes Hirrlinger, Maarten Hp Kole, Ryuichi Shigemoto, and Stefan Hallermann. “HCN Channel-Mediated Neuromodulation Can Control Action Potential Velocity and Fidelity in Central Axons.” <i>ELife</i>. eLife Sciences Publications, 2019. <a href=\"https://doi.org/10.7554/eLife.42766\">https://doi.org/10.7554/eLife.42766</a>.","mla":"Byczkowicz, Niklas, et al. “HCN Channel-Mediated Neuromodulation Can Control Action Potential Velocity and Fidelity in Central Axons.” <i>ELife</i>, vol. 8, e42766, eLife Sciences Publications, 2019, doi:<a href=\"https://doi.org/10.7554/eLife.42766\">10.7554/eLife.42766</a>."}},{"volume":104,"date_updated":"2023-08-30T07:28:22Z","ddc":["571","599"],"date_published":"2019-11-20T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.neuron.2019.08.013"}],"date_created":"2019-11-25T08:02:39Z","year":"2019","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","intvolume":"       104","publication":"Neuron","doi":"10.1016/j.neuron.2019.08.013","external_id":{"pmid":["31543297"],"isi":["000497963500017"]},"department":[{"_id":"RySh"}],"publication_status":"published","author":[{"full_name":"Kasugai, Yu","last_name":"Kasugai","first_name":"Yu"},{"first_name":"Elisabeth","last_name":"Vogel","full_name":"Vogel, Elisabeth"},{"last_name":"Hörtnagl","full_name":"Hörtnagl, Heide","first_name":"Heide"},{"last_name":"Schönherr","full_name":"Schönherr, Sabine","first_name":"Sabine"},{"full_name":"Paradiso, Enrica","last_name":"Paradiso","first_name":"Enrica"},{"first_name":"Markus","full_name":"Hauschild, Markus","last_name":"Hauschild"},{"first_name":"Georg","full_name":"Göbel, Georg","last_name":"Göbel"},{"first_name":"Ivan","full_name":"Milenkovic, Ivan","last_name":"Milenkovic"},{"full_name":"Peterschmitt, Yvan","last_name":"Peterschmitt","first_name":"Yvan"},{"first_name":"Ramon","full_name":"Tasan, Ramon","last_name":"Tasan"},{"first_name":"Günther","last_name":"Sperk","full_name":"Sperk, Günther"},{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi"},{"full_name":"Sieghart, Werner","last_name":"Sieghart","first_name":"Werner"},{"first_name":"Nicolas","full_name":"Singewald, Nicolas","last_name":"Singewald"},{"full_name":"Lüthi, Andreas","last_name":"Lüthi","first_name":"Andreas"},{"last_name":"Ferraguti","full_name":"Ferraguti, Francesco","first_name":"Francesco"}],"language":[{"iso":"eng"}],"oa":1,"issue":"4","_id":"7099","article_type":"original","article_processing_charge":"No","publication_identifier":{"issn":["0896-6273"]},"publisher":"Elsevier","quality_controlled":"1","month":"11","page":"781-794.e4","status":"public","type":"journal_article","day":"20","has_accepted_license":"1","citation":{"apa":"Kasugai, Y., Vogel, E., Hörtnagl, H., Schönherr, S., Paradiso, E., Hauschild, M., … Ferraguti, F. (2019). Structural and functional remodeling of amygdala GABAergic synapses in associative fear learning. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2019.08.013\">https://doi.org/10.1016/j.neuron.2019.08.013</a>","ista":"Kasugai Y, Vogel E, Hörtnagl H, Schönherr S, Paradiso E, Hauschild M, Göbel G, Milenkovic I, Peterschmitt Y, Tasan R, Sperk G, Shigemoto R, Sieghart W, Singewald N, Lüthi A, Ferraguti F. 2019. Structural and functional remodeling of amygdala GABAergic synapses in associative fear learning. Neuron. 104(4), 781–794.e4.","short":"Y. Kasugai, E. Vogel, H. Hörtnagl, S. Schönherr, E. Paradiso, M. Hauschild, G. Göbel, I. Milenkovic, Y. Peterschmitt, R. Tasan, G. Sperk, R. Shigemoto, W. Sieghart, N. Singewald, A. Lüthi, F. Ferraguti, Neuron 104 (2019) 781–794.e4.","ama":"Kasugai Y, Vogel E, Hörtnagl H, et al. Structural and functional remodeling of amygdala GABAergic synapses in associative fear learning. <i>Neuron</i>. 2019;104(4):781-794.e4. doi:<a href=\"https://doi.org/10.1016/j.neuron.2019.08.013\">10.1016/j.neuron.2019.08.013</a>","ieee":"Y. Kasugai <i>et al.</i>, “Structural and functional remodeling of amygdala GABAergic synapses in associative fear learning,” <i>Neuron</i>, vol. 104, no. 4. Elsevier, p. 781–794.e4, 2019.","chicago":"Kasugai, Yu, Elisabeth Vogel, Heide Hörtnagl, Sabine Schönherr, Enrica Paradiso, Markus Hauschild, Georg Göbel, et al. “Structural and Functional Remodeling of Amygdala GABAergic Synapses in Associative Fear Learning.” <i>Neuron</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.neuron.2019.08.013\">https://doi.org/10.1016/j.neuron.2019.08.013</a>.","mla":"Kasugai, Yu, et al. “Structural and Functional Remodeling of Amygdala GABAergic Synapses in Associative Fear Learning.” <i>Neuron</i>, vol. 104, no. 4, Elsevier, 2019, p. 781–794.e4, doi:<a href=\"https://doi.org/10.1016/j.neuron.2019.08.013\">10.1016/j.neuron.2019.08.013</a>."},"pmid":1,"isi":1,"acknowledgement":"The authors thank Gabi Schmid for excellent technical support. We also thank\r\nDr. H. Harada, Dr. W. Kaufmann, and Dr. B. Kapelari for testing the specificity\r\nof some of the antibodies used in this study on replicas. Funding was provided\r\nby the Austrian Science Fund (Fonds zur Fo¨ rderung der Wissenschaftlichen\r\nForschung) Sonderforschungsbereich grants F44-17 (to F.jF.), F44-10 and\r\nP25375-B24 (to N.S.), and P26680 (to G.S.) and by the Novartis Research\r\nFoundation and the Swiss National Science Foundation (to A.L). We also thank\r\nProf. M. Capogna for reading a previous version of the manuscript.","scopus_import":"1","oa_version":"Published Version","title":"Structural and functional remodeling of amygdala GABAergic synapses in associative fear learning"},{"intvolume":"        33","file":[{"creator":"shigemot","access_level":"open_access","date_updated":"2020-12-06T17:30:09Z","file_size":4766789,"file_name":"Klotz et al 2019 EMBO Reports.pdf","file_id":"8922","checksum":"79e3b72481dc32489911121cf3b7d8d0","relation":"main_file","date_created":"2020-12-06T17:30:09Z","success":1,"content_type":"application/pdf"}],"publication":"FASEB Journal","file_date_updated":"2020-12-06T17:30:09Z","doi":"10.1096/fj.201901543R","abstract":[{"text":"Glutamate is the major excitatory neurotransmitter in the CNS binding to a variety of glutamate receptors. Metabotropic glutamate receptors (mGluR1 to mGluR8) can act excitatory or inhibitory, depending on associated signal cascades. Expression and localization of inhibitory acting mGluRs at inner hair cells (IHCs) in the cochlea are largely unknown. Here, we analyzed expression of mGluR2, mGluR3, mGluR4, mGluR6, mGluR7, and mGluR8 and investigated their localization with respect to the presynaptic ribbon of IHC synapses. We detected transcripts for mGluR2, mGluR3, and mGluR4 as well as for mGluR7a, mGluR7b, mGluR8a, and mGluR8b splice variants. Using receptor-specific antibodies in cochlear wholemounts, we found expression of mGluR2, mGluR4, and mGluR8b close to presynaptic ribbons. Super resolution and confocal microscopy in combination with 3-dimensional reconstructions indicated a postsynaptic localization of mGluR2 that overlaps with postsynaptic density protein 95 on dendrites of afferent type I spiral ganglion neurons. In contrast, mGluR4 and mGluR8b were expressed at the presynapse close to IHC ribbons. In summary, we localized in detail 3 mGluR types at IHC ribbon synapses, providing a fundament for new therapeutical strategies that could protect the cochlea against noxious stimuli and excitotoxicity.","lang":"eng"}],"department":[{"_id":"RySh"}],"external_id":{"isi":["000507466100054"],"pmid":["31585509"]},"publication_status":"published","date_updated":"2026-04-03T09:44:03Z","volume":33,"date_published":"2019-12-01T00:00:00Z","ddc":["571","599"],"date_created":"2019-12-15T23:00:42Z","year":"2019","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","has_accepted_license":"1","citation":{"ista":"Klotz L, Wendler O, Frischknecht R, Shigemoto R, Schulze H, Enz R. 2019. Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses. FASEB Journal. 33(12), 13734–13746.","apa":"Klotz, L., Wendler, O., Frischknecht, R., Shigemoto, R., Schulze, H., &#38; Enz, R. (2019). Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses. <i>FASEB Journal</i>. FASEB. <a href=\"https://doi.org/10.1096/fj.201901543R\">https://doi.org/10.1096/fj.201901543R</a>","short":"L. Klotz, O. Wendler, R. Frischknecht, R. Shigemoto, H. Schulze, R. Enz, FASEB Journal 33 (2019) 13734–13746.","ama":"Klotz L, Wendler O, Frischknecht R, Shigemoto R, Schulze H, Enz R. Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses. <i>FASEB Journal</i>. 2019;33(12):13734-13746. doi:<a href=\"https://doi.org/10.1096/fj.201901543R\">10.1096/fj.201901543R</a>","ieee":"L. Klotz, O. Wendler, R. Frischknecht, R. Shigemoto, H. Schulze, and R. Enz, “Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses,” <i>FASEB Journal</i>, vol. 33, no. 12. FASEB, pp. 13734–13746, 2019.","mla":"Klotz, Lisa, et al. “Localization of Group II and III Metabotropic Glutamate Receptors at Pre- and Postsynaptic Sites of Inner Hair Cell Ribbon Synapses.” <i>FASEB Journal</i>, vol. 33, no. 12, FASEB, 2019, pp. 13734–46, doi:<a href=\"https://doi.org/10.1096/fj.201901543R\">10.1096/fj.201901543R</a>.","chicago":"Klotz, Lisa, Olaf Wendler, Renato Frischknecht, Ryuichi Shigemoto, Holger Schulze, and Ralf Enz. “Localization of Group II and III Metabotropic Glutamate Receptors at Pre- and Postsynaptic Sites of Inner Hair Cell Ribbon Synapses.” <i>FASEB Journal</i>. FASEB, 2019. <a href=\"https://doi.org/10.1096/fj.201901543R\">https://doi.org/10.1096/fj.201901543R</a>."},"pmid":1,"isi":1,"oa_version":"Submitted Version","scopus_import":"1","title":"Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses","author":[{"first_name":"Lisa","last_name":"Klotz","full_name":"Klotz, Lisa"},{"full_name":"Wendler, Olaf","last_name":"Wendler","first_name":"Olaf"},{"full_name":"Frischknecht, Renato","last_name":"Frischknecht","first_name":"Renato"},{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi"},{"last_name":"Schulze","full_name":"Schulze, Holger","first_name":"Holger"},{"first_name":"Ralf","last_name":"Enz","full_name":"Enz, Ralf"}],"oa":1,"language":[{"iso":"eng"}],"_id":"7179","issue":"12","article_processing_charge":"No","article_type":"original","publication_identifier":{"eissn":["1530-6860"]},"publisher":"FASEB","month":"12","quality_controlled":"1","status":"public","page":"13734-13746","day":"01","type":"journal_article"},{"date_published":"2019-07-03T00:00:00Z","ddc":["570"],"date_updated":"2023-09-07T14:52:23Z","tmp":{"image":"/images/cc_by_nc_sa.png","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","short":"CC BY-NC-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode"},"volume":151,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_created":"2020-01-29T16:06:29Z","year":"2019","file_date_updated":"2020-07-14T12:47:57Z","publication":"The Journal of General Physiology","intvolume":"       151","file":[{"relation":"main_file","checksum":"5706b4ccd74ee3e50bf7ecb2a203df71","file_id":"7450","content_type":"application/pdf","date_created":"2020-02-05T07:20:32Z","creator":"dernst","date_updated":"2020-07-14T12:47:57Z","file_name":"2019_JGP_Erdem.pdf","file_size":2641297,"access_level":"open_access"}],"department":[{"_id":"RySh"}],"external_id":{"isi":["000478792500008"],"pmid":["31270129"]},"publication_status":"published","doi":"10.1085/jgp.201912318","abstract":[{"lang":"eng","text":"Transporters of the solute carrier 6 (SLC6) family translocate their cognate substrate together with Na+ and Cl−. Detailed kinetic models exist for the transporters of GABA (GAT1/SLC6A1) and the monoamines dopamine (DAT/SLC6A3) and serotonin (SERT/SLC6A4). Here, we posited that the transport cycle of individual SLC6 transporters reflects the physiological requirements they operate under. We tested this hypothesis by analyzing the transport cycle of glycine transporter 1 (GlyT1/SLC6A9) and glycine transporter 2 (GlyT2/SLC6A5). GlyT2 is the only SLC6 family member known to translocate glycine, Na+, and Cl− in a 1:3:1 stoichiometry. We analyzed partial reactions in real time by electrophysiological recordings. Contrary to monoamine transporters, both GlyTs were found to have a high transport capacity driven by rapid return of the empty transporter after release of Cl− on the intracellular side. Rapid cycling of both GlyTs was further supported by highly cooperative binding of cosubstrate ions and substrate such that their forward transport mode was maintained even under conditions of elevated intracellular Na+ or Cl−. The most important differences in the transport cycle of GlyT1 and GlyT2 arose from the kinetics of charge movement and the resulting voltage-dependent rate-limiting reactions: the kinetics of GlyT1 were governed by transition of the substrate-bound transporter from outward- to inward-facing conformations, whereas the kinetics of GlyT2 were governed by Na+ binding (or a related conformational change). Kinetic modeling showed that the kinetics of GlyT1 are ideally suited for supplying the extracellular glycine levels required for NMDA receptor activation."}],"_id":"7398","issue":"8","article_processing_charge":"No","article_type":"original","author":[{"first_name":"Fatma Asli","last_name":"Erdem","full_name":"Erdem, Fatma Asli"},{"first_name":"Marija","last_name":"Ilic","full_name":"Ilic, Marija"},{"first_name":"Peter","id":"3B8B25A8-F248-11E8-B48F-1D18A9856A87","last_name":"Koppensteiner","full_name":"Koppensteiner, Peter","orcid":"0000-0002-3509-1948"},{"last_name":"Gołacki","full_name":"Gołacki, Jakub","first_name":"Jakub"},{"last_name":"Lubec","full_name":"Lubec, Gert","first_name":"Gert"},{"first_name":"Michael","full_name":"Freissmuth, Michael","last_name":"Freissmuth"},{"last_name":"Sandtner","full_name":"Sandtner, Walter","first_name":"Walter"}],"oa":1,"language":[{"iso":"eng"}],"status":"public","page":"1035-1050","day":"03","type":"journal_article","publisher":"Rockefeller University Press","publication_identifier":{"issn":["0022-1295"],"eissn":["1540-7748"]},"quality_controlled":"1","month":"07","pmid":1,"has_accepted_license":"1","citation":{"mla":"Erdem, Fatma Asli, et al. “A Comparison of the Transport Kinetics of Glycine Transporter 1 and Glycine Transporter 2.” <i>The Journal of General Physiology</i>, vol. 151, no. 8, Rockefeller University Press, 2019, pp. 1035–50, doi:<a href=\"https://doi.org/10.1085/jgp.201912318\">10.1085/jgp.201912318</a>.","chicago":"Erdem, Fatma Asli, Marija Ilic, Peter Koppensteiner, Jakub Gołacki, Gert Lubec, Michael Freissmuth, and Walter Sandtner. “A Comparison of the Transport Kinetics of Glycine Transporter 1 and Glycine Transporter 2.” <i>The Journal of General Physiology</i>. Rockefeller University Press, 2019. <a href=\"https://doi.org/10.1085/jgp.201912318\">https://doi.org/10.1085/jgp.201912318</a>.","apa":"Erdem, F. A., Ilic, M., Koppensteiner, P., Gołacki, J., Lubec, G., Freissmuth, M., &#38; Sandtner, W. (2019). A comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2. <i>The Journal of General Physiology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1085/jgp.201912318\">https://doi.org/10.1085/jgp.201912318</a>","ista":"Erdem FA, Ilic M, Koppensteiner P, Gołacki J, Lubec G, Freissmuth M, Sandtner W. 2019. A comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2. The Journal of General Physiology. 151(8), 1035–1050.","ieee":"F. A. Erdem <i>et al.</i>, “A comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2,” <i>The Journal of General Physiology</i>, vol. 151, no. 8. Rockefeller University Press, pp. 1035–1050, 2019.","short":"F.A. Erdem, M. Ilic, P. Koppensteiner, J. Gołacki, G. Lubec, M. Freissmuth, W. Sandtner, The Journal of General Physiology 151 (2019) 1035–1050.","ama":"Erdem FA, Ilic M, Koppensteiner P, et al. A comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2. <i>The Journal of General Physiology</i>. 2019;151(8):1035-1050. doi:<a href=\"https://doi.org/10.1085/jgp.201912318\">10.1085/jgp.201912318</a>"},"title":"A comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2","isi":1,"scopus_import":"1","oa_version":"Published Version"},{"year":"2019","date_created":"2020-01-29T15:56:56Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"volume":22,"date_updated":"2026-08-09T22:30:06Z","project":[{"call_identifier":"H2020","grant_number":"694539","_id":"25CA28EA-B435-11E9-9278-68D0E5697425","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour"},{"_id":"25CBA828-B435-11E9-9278-68D0E5697425","grant_number":"720270","call_identifier":"H2020","name":"Human Brain Project Specific Grant Agreement 1"}],"ddc":["570"],"date_published":"2019-12-20T00:00:00Z","ec_funded":1,"abstract":[{"text":"Electron microscopy (EM) is a technology that enables visualization of single proteins at a nanometer resolution. However, current protein analysis by EM mainly relies on immunolabeling with gold-particle-conjugated antibodies, which is compromised by large size of antibody, precluding precise detection of protein location in biological samples. Here, we develop a specific chemical labeling method for EM detection of proteins at single-molecular level. Rational design of α-helical peptide tag and probe structure provided a complementary reaction pair that enabled specific cysteine conjugation of the tag. The developed chemical labeling with gold-nanoparticle-conjugated probe showed significantly higher labeling efficiency and detectability of high-density clusters of tag-fused G protein-coupled receptors in freeze-fracture replicas compared with immunogold labeling. Furthermore, in ultrathin sections, the spatial resolution of the chemical labeling was significantly higher than that of antibody-mediated labeling. These results demonstrate substantial advantages of the chemical labeling approach for single protein visualization by EM.","lang":"eng"}],"doi":"10.1016/j.isci.2019.11.025","publication_status":"published","external_id":{"isi":["000504652000020"],"pmid":["31786521"]},"department":[{"_id":"RySh"}],"publication":"iScience","file":[{"file_id":"7448","checksum":"f3e90056a49f09b205b1c4f8c739ffd1","relation":"main_file","date_created":"2020-02-04T10:48:36Z","content_type":"application/pdf","creator":"dernst","access_level":"open_access","file_size":7197776,"file_name":"2019_iScience_Tabata.pdf","date_updated":"2020-07-14T12:47:57Z"}],"intvolume":"        22","file_date_updated":"2020-07-14T12:47:57Z","quality_controlled":"1","month":"12","publication_identifier":{"issn":["2589-0042"]},"publisher":"Elsevier","type":"journal_article","day":"20","status":"public","page":"256-268","language":[{"iso":"eng"}],"oa":1,"author":[{"first_name":"Shigekazu","last_name":"Tabata","full_name":"Tabata, Shigekazu","id":"4427179E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Jevtic, Marijo","last_name":"Jevtic","id":"4BE3BC94-F248-11E8-B48F-1D18A9856A87","first_name":"Marijo"},{"first_name":"Nobutaka","full_name":"Kurashige, Nobutaka","last_name":"Kurashige"},{"full_name":"Fuchida, Hirokazu","last_name":"Fuchida","first_name":"Hirokazu"},{"first_name":"Munetsugu","full_name":"Kido, Munetsugu","last_name":"Kido"},{"first_name":"Kazushi","full_name":"Tani, Kazushi","last_name":"Tani"},{"first_name":"Naoki","full_name":"Zenmyo, Naoki","last_name":"Zenmyo"},{"first_name":"Shohei","full_name":"Uchinomiya, Shohei","last_name":"Uchinomiya"},{"last_name":"Harada","full_name":"Harada, Harumi","orcid":"0000-0001-7429-7896","id":"2E55CDF2-F248-11E8-B48F-1D18A9856A87","first_name":"Harumi"},{"last_name":"Itakura","full_name":"Itakura, Makoto","first_name":"Makoto"},{"first_name":"Itaru","full_name":"Hamachi, Itaru","last_name":"Hamachi"},{"first_name":"Ryuichi","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ojida, Akio","last_name":"Ojida","first_name":"Akio"}],"article_processing_charge":"No","article_type":"original","corr_author":"1","_id":"7391","issue":"12","scopus_import":"1","oa_version":"Published Version","isi":1,"related_material":{"record":[{"id":"11393","relation":"dissertation_contains","status":"public"}]},"title":"Electron microscopic detection of single membrane proteins by a specific chemical labeling","citation":{"chicago":"Tabata, Shigekazu, Marijo Jevtic, Nobutaka Kurashige, Hirokazu Fuchida, Munetsugu Kido, Kazushi Tani, Naoki Zenmyo, et al. “Electron Microscopic Detection of Single Membrane Proteins by a Specific Chemical Labeling.” <i>IScience</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.isci.2019.11.025\">https://doi.org/10.1016/j.isci.2019.11.025</a>.","mla":"Tabata, Shigekazu, et al. “Electron Microscopic Detection of Single Membrane Proteins by a Specific Chemical Labeling.” <i>IScience</i>, vol. 22, no. 12, Elsevier, 2019, pp. 256–68, doi:<a href=\"https://doi.org/10.1016/j.isci.2019.11.025\">10.1016/j.isci.2019.11.025</a>.","apa":"Tabata, S., Jevtic, M., Kurashige, N., Fuchida, H., Kido, M., Tani, K., … Ojida, A. (2019). Electron microscopic detection of single membrane proteins by a specific chemical labeling. <i>IScience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.isci.2019.11.025\">https://doi.org/10.1016/j.isci.2019.11.025</a>","ista":"Tabata S, Jevtic M, Kurashige N, Fuchida H, Kido M, Tani K, Zenmyo N, Uchinomiya S, Harada H, Itakura M, Hamachi I, Shigemoto R, Ojida A. 2019. Electron microscopic detection of single membrane proteins by a specific chemical labeling. iScience. 22(12), 256–268.","ieee":"S. Tabata <i>et al.</i>, “Electron microscopic detection of single membrane proteins by a specific chemical labeling,” <i>iScience</i>, vol. 22, no. 12. Elsevier, pp. 256–268, 2019.","short":"S. Tabata, M. Jevtic, N. Kurashige, H. Fuchida, M. Kido, K. Tani, N. Zenmyo, S. Uchinomiya, H. Harada, M. Itakura, I. Hamachi, R. Shigemoto, A. Ojida, IScience 22 (2019) 256–268.","ama":"Tabata S, Jevtic M, Kurashige N, et al. Electron microscopic detection of single membrane proteins by a specific chemical labeling. <i>iScience</i>. 2019;22(12):256-268. doi:<a href=\"https://doi.org/10.1016/j.isci.2019.11.025\">10.1016/j.isci.2019.11.025</a>"},"has_accepted_license":"1","pmid":1},{"intvolume":"        66","publication":"Journal of Histochemistry and Cytochemistry","publication_status":"published","external_id":{"isi":["000452277700005"],"pmid":["29969056"]},"department":[{"_id":"RySh"},{"_id":"EM-Fac"}],"abstract":[{"text":"For ultrafast fixation of biological samples to avoid artifacts, high-pressure freezing (HPF) followed by freeze substitution (FS) is preferred over chemical fixation at room temperature. After HPF, samples are maintained at low temperature during dehydration and fixation, while avoiding damaging recrystallization. This is a notoriously slow process. McDonald and Webb demonstrated, in 2011, that sample agitation during FS dramatically reduces the necessary time. Then, in 2015, we (H.G. and S.R.) introduced an agitation module into the cryochamber of an automated FS unit and demonstrated that the preparation of algae could be shortened from days to a couple of hours. We argued that variability in the processing, reproducibility, and safety issues are better addressed using automated FS units. For dissemination, we started low-cost manufacturing of agitation modules for two of the most widely used FS units, the Automatic Freeze Substitution Systems, AFS(1) and AFS2, from Leica Microsystems, using three dimensional (3D)-printing of the major components. To test them, several labs independently used the modules on a wide variety of specimens that had previously been processed by manual agitation, or without agitation. We demonstrate that automated processing with sample agitation saves time, increases flexibility with respect to sample requirements and protocols, and produces data of at least as good quality as other approaches.","lang":"eng"}],"doi":"10.1369/0022155418786698","ddc":["570"],"date_published":"2018-12-01T00:00:00Z","volume":66,"date_updated":"2026-06-18T17:50:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2018","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1369/0022155418786698"}],"date_created":"2018-12-11T11:44:57Z","pmid":1,"citation":{"apa":"Reipert, S., Goldammer, H., Richardson, C., Goldberg, M., Hawkins, T., Saeckl, E., … Stierhof, Y. (2018). Agitation modules: Flexible means to accelerate automated freeze substitution. <i>Journal of Histochemistry and Cytochemistry</i>. SAGE Publications. <a href=\"https://doi.org/10.1369/0022155418786698\">https://doi.org/10.1369/0022155418786698</a>","ista":"Reipert S, Goldammer H, Richardson C, Goldberg M, Hawkins T, Saeckl E, Kaufmann W, Antreich S, Stierhof Y. 2018. Agitation modules: Flexible means to accelerate automated freeze substitution. Journal of Histochemistry and Cytochemistry. 66(12), 903–921.","ama":"Reipert S, Goldammer H, Richardson C, et al. Agitation modules: Flexible means to accelerate automated freeze substitution. <i>Journal of Histochemistry and Cytochemistry</i>. 2018;66(12):903-921. doi:<a href=\"https://doi.org/10.1369/0022155418786698\">10.1369/0022155418786698</a>","ieee":"S. Reipert <i>et al.</i>, “Agitation modules: Flexible means to accelerate automated freeze substitution,” <i>Journal of Histochemistry and Cytochemistry</i>, vol. 66, no. 12. SAGE Publications, pp. 903–921, 2018.","short":"S. Reipert, H. Goldammer, C. Richardson, M. Goldberg, T. Hawkins, E. Saeckl, W. Kaufmann, S. Antreich, Y. Stierhof, Journal of Histochemistry and Cytochemistry 66 (2018) 903–921.","mla":"Reipert, Siegfried, et al. “Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution.” <i>Journal of Histochemistry and Cytochemistry</i>, vol. 66, no. 12, SAGE Publications, 2018, pp. 903–21, doi:<a href=\"https://doi.org/10.1369/0022155418786698\">10.1369/0022155418786698</a>.","chicago":"Reipert, Siegfried, Helmuth Goldammer, Christine Richardson, Martin Goldberg, Timothy Hawkins, Elena Saeckl, Walter Kaufmann, Sebastian Antreich, and York Stierhof. “Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution.” <i>Journal of Histochemistry and Cytochemistry</i>. SAGE Publications, 2018. <a href=\"https://doi.org/10.1369/0022155418786698\">https://doi.org/10.1369/0022155418786698</a>."},"title":"Agitation modules: Flexible means to accelerate automated freeze substitution","scopus_import":"1","oa_version":"Published Version","isi":1,"article_type":"original","article_processing_charge":"No","_id":"163","issue":"12","language":[{"iso":"eng"}],"oa":1,"author":[{"first_name":"Siegfried","full_name":"Reipert, Siegfried","last_name":"Reipert"},{"last_name":"Goldammer","full_name":"Goldammer, Helmuth","first_name":"Helmuth"},{"full_name":"Richardson, Christine","last_name":"Richardson","first_name":"Christine"},{"first_name":"Martin","last_name":"Goldberg","full_name":"Goldberg, Martin"},{"last_name":"Hawkins","full_name":"Hawkins, Timothy","first_name":"Timothy"},{"full_name":"Hollergschwandtner, Elena","last_name":"Hollergschwandtner","id":"3C054040-F248-11E8-B48F-1D18A9856A87","first_name":"Elena"},{"full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter"},{"full_name":"Antreich, Sebastian","last_name":"Antreich","first_name":"Sebastian"},{"full_name":"Stierhof, York","last_name":"Stierhof","first_name":"York"}],"type":"journal_article","day":"01","page":"903-921","status":"public","month":"12","quality_controlled":"1","publication_identifier":{"issn":["0022-1554"]},"publisher":"SAGE Publications"},{"day":"01","type":"book_chapter","status":"public","page":"201 - 215","quality_controlled":"1","month":"01","publisher":"Springer","article_processing_charge":"No","_id":"562","oa":1,"language":[{"iso":"eng"}],"author":[{"full_name":"Dimitrov, Dimitar","last_name":"Dimitrov","first_name":"Dimitar"},{"first_name":"Laurent","last_name":"Guillaud","full_name":"Guillaud, Laurent"},{"first_name":"Kohgaku","id":"2B7846DC-F248-11E8-B48F-1D18A9856A87","last_name":"Eguchi","orcid":"0000-0002-6170-2546","full_name":"Eguchi, Kohgaku"},{"full_name":"Takahashi, Tomoyuki","last_name":"Takahashi","first_name":"Tomoyuki"}],"title":"Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses","oa_version":"Submitted Version","scopus_import":1,"pmid":1,"citation":{"chicago":"Dimitrov, Dimitar, Laurent Guillaud, Kohgaku Eguchi, and Tomoyuki Takahashi. “Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses.” In <i>Neurotrophic Factors</i>, edited by Stephen D. Skaper, 1727:201–15. Springer, 2018. <a href=\"https://doi.org/10.1007/978-1-4939-7571-6_15\">https://doi.org/10.1007/978-1-4939-7571-6_15</a>.","mla":"Dimitrov, Dimitar, et al. “Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses.” <i>Neurotrophic Factors</i>, edited by Stephen D. Skaper, vol. 1727, Springer, 2018, pp. 201–15, doi:<a href=\"https://doi.org/10.1007/978-1-4939-7571-6_15\">10.1007/978-1-4939-7571-6_15</a>.","ista":"Dimitrov D, Guillaud L, Eguchi K, Takahashi T. 2018.Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses. In: Neurotrophic Factors. Methods in Molecular Biology, vol. 1727, 201–215.","apa":"Dimitrov, D., Guillaud, L., Eguchi, K., &#38; Takahashi, T. (2018). Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses. In S. D. Skaper (Ed.), <i>Neurotrophic Factors</i> (Vol. 1727, pp. 201–215). Springer. <a href=\"https://doi.org/10.1007/978-1-4939-7571-6_15\">https://doi.org/10.1007/978-1-4939-7571-6_15</a>","short":"D. Dimitrov, L. Guillaud, K. Eguchi, T. Takahashi, in:, S.D. Skaper (Ed.), Neurotrophic Factors, Springer, 2018, pp. 201–215.","ieee":"D. Dimitrov, L. Guillaud, K. Eguchi, and T. Takahashi, “Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses,” in <i>Neurotrophic Factors</i>, vol. 1727, S. D. Skaper, Ed. Springer, 2018, pp. 201–215.","ama":"Dimitrov D, Guillaud L, Eguchi K, Takahashi T. Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses. In: Skaper SD, ed. <i>Neurotrophic Factors</i>. Vol 1727. Springer; 2018:201-215. doi:<a href=\"https://doi.org/10.1007/978-1-4939-7571-6_15\">10.1007/978-1-4939-7571-6_15</a>"},"has_accepted_license":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publist_id":"7252","year":"2018","date_created":"2018-12-11T11:47:11Z","editor":[{"full_name":"Skaper, Stephen D.","last_name":"Skaper","first_name":"Stephen D."}],"date_published":"2018-01-01T00:00:00Z","ddc":["570"],"date_updated":"2021-01-12T08:03:05Z","volume":1727,"publication_status":"published","department":[{"_id":"RySh"}],"external_id":{"pmid":["29222783"]},"abstract":[{"text":"Primary neuronal cell culture preparations are widely used to investigate synaptic functions. This chapter describes a detailed protocol for the preparation of a neuronal cell culture in which giant calyx-type synaptic terminals are formed. This chapter also presents detailed protocols for utilizing the main technical advantages provided by such a preparation, namely, labeling and imaging of synaptic organelles and electrophysiological recordings directly from presynaptic terminals.","lang":"eng"}],"doi":"10.1007/978-1-4939-7571-6_15","alternative_title":["Methods in Molecular Biology"],"file_date_updated":"2020-07-14T12:47:09Z","publication":"Neurotrophic Factors","file":[{"creator":"dernst","access_level":"open_access","date_updated":"2020-07-14T12:47:09Z","file_name":"2018_NeurotrophicFactors_Dimitrov.pdf","file_size":787407,"file_id":"7046","checksum":"8aa174ca65a56fbb19e9f88cff3ac3fd","relation":"main_file","date_created":"2019-11-19T07:47:43Z","content_type":"application/pdf"}],"intvolume":"      1727"},{"publication":"Molecular Neurobiology","intvolume":"        55","abstract":[{"text":"Although dopamine receptors D1 and D2 play key roles in hippocampal function, their synaptic localization within the hippocampus has not been fully elucidated. In order to understand precise functions of pre- or postsynaptic dopamine receptors (DRs), the development of protocols to differentiate pre- and postsynaptic DRs is essential. So far, most studies on determination and quantification of DRs did not discriminate between subsynaptic localization. Therefore, the aim of the study was to generate a robust workflow for the localization of DRs. This work provides the basis for future work on hippocampal DRs, in light that DRs may have different functions at pre- or postsynaptic sites. Synaptosomes from rat hippocampi isolated by a sucrose gradient protocol were prepared for super-resolution direct stochastic optical reconstruction microscopy (dSTORM) using Bassoon as a presynaptic zone and Homer1 as postsynaptic density marker. Direct labeling of primary validated antibodies against dopamine receptors D1 (D1R) and D2 (D2R) with Alexa Fluor 594 enabled unequivocal assignment of D1R and D2R to both, pre- and postsynaptic sites. D1R immunoreactivity clusters were observed within the presynaptic active zone as well as at perisynaptic sites at the edge of the presynaptic active zone. The results may be useful for the interpretation of previous studies and the design of future work on DRs in the hippocampus. Moreover, the reduction of the complexity of brain tissue by the use of synaptosomal preparations and dSTORM technology may represent a useful tool for synaptic localization of brain proteins.","lang":"eng"}],"doi":"10.1007/s12035-017-0688-y","publication_status":"published","department":[{"_id":"RySh"}],"external_id":{"isi":["000431991500025"]},"date_updated":"2023-09-19T09:58:11Z","volume":55,"date_published":"2018-06-01T00:00:00Z","publist_id":"6991","year":"2018","date_created":"2018-12-11T11:48:02Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"ama":"Miklosi A, Del Favero G, Bulat T, et al. Super resolution microscopical localization of dopamine receptors 1 and 2 in rat hippocampal synaptosomes. <i>Molecular Neurobiology</i>. 2018;55(6):4857 – 4869. doi:<a href=\"https://doi.org/10.1007/s12035-017-0688-y\">10.1007/s12035-017-0688-y</a>","ieee":"A. Miklosi <i>et al.</i>, “Super resolution microscopical localization of dopamine receptors 1 and 2 in rat hippocampal synaptosomes,” <i>Molecular Neurobiology</i>, vol. 55, no. 6. Springer, pp. 4857 – 4869, 2018.","short":"A. Miklosi, G. Del Favero, T. Bulat, H. Höger, R. Shigemoto, D. Marko, G. Lubec, Molecular Neurobiology 55 (2018) 4857 – 4869.","ista":"Miklosi A, Del Favero G, Bulat T, Höger H, Shigemoto R, Marko D, Lubec G. 2018. Super resolution microscopical localization of dopamine receptors 1 and 2 in rat hippocampal synaptosomes. Molecular Neurobiology. 55(6), 4857 – 4869.","apa":"Miklosi, A., Del Favero, G., Bulat, T., Höger, H., Shigemoto, R., Marko, D., &#38; Lubec, G. (2018). Super resolution microscopical localization of dopamine receptors 1 and 2 in rat hippocampal synaptosomes. <i>Molecular Neurobiology</i>. Springer. <a href=\"https://doi.org/10.1007/s12035-017-0688-y\">https://doi.org/10.1007/s12035-017-0688-y</a>","chicago":"Miklosi, Andras, Giorgia Del Favero, Tanja Bulat, Harald Höger, Ryuichi Shigemoto, Doris Marko, and Gert Lubec. “Super Resolution Microscopical Localization of Dopamine Receptors 1 and 2 in Rat Hippocampal Synaptosomes.” <i>Molecular Neurobiology</i>. Springer, 2018. <a href=\"https://doi.org/10.1007/s12035-017-0688-y\">https://doi.org/10.1007/s12035-017-0688-y</a>.","mla":"Miklosi, Andras, et al. “Super Resolution Microscopical Localization of Dopamine Receptors 1 and 2 in Rat Hippocampal Synaptosomes.” <i>Molecular Neurobiology</i>, vol. 55, no. 6, Springer, 2018, pp. 4857 – 4869, doi:<a href=\"https://doi.org/10.1007/s12035-017-0688-y\">10.1007/s12035-017-0688-y</a>."},"scopus_import":"1","oa_version":"None","isi":1,"title":"Super resolution microscopical localization of dopamine receptors 1 and 2 in rat hippocampal synaptosomes","language":[{"iso":"eng"}],"author":[{"last_name":"Miklosi","full_name":"Miklosi, Andras","first_name":"Andras"},{"last_name":"Del Favero","full_name":"Del Favero, Giorgia","first_name":"Giorgia"},{"first_name":"Tanja","last_name":"Bulat","full_name":"Bulat, Tanja"},{"last_name":"Höger","full_name":"Höger, Harald","first_name":"Harald"},{"first_name":"Ryuichi","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Marko","full_name":"Marko, Doris","first_name":"Doris"},{"first_name":"Gert","full_name":"Lubec, Gert","last_name":"Lubec"}],"article_processing_charge":"No","issue":"6","_id":"705","quality_controlled":"1","month":"06","publisher":"Springer","day":"01","type":"journal_article","status":"public","page":"4857 – 4869"},{"has_accepted_license":"1","citation":{"mla":"Sawada, Kazuaki, et al. “Super Resolution Structural Analysis of Dendritic Spines Using Three-Dimensional Structured Illumination Microscopy in Cleared Mouse Brain Slices.” <i>European Journal of Neuroscience</i>, vol. 47, no. 9, Wiley, 2018, pp. 1033–42, doi:<a href=\"https://doi.org/10.1111/ejn.13901\">10.1111/ejn.13901</a>.","chicago":"Sawada, Kazuaki, Ryosuke Kawakami, Ryuichi Shigemoto, and Tomomi Nemoto. “Super Resolution Structural Analysis of Dendritic Spines Using Three-Dimensional Structured Illumination Microscopy in Cleared Mouse Brain Slices.” <i>European Journal of Neuroscience</i>. Wiley, 2018. <a href=\"https://doi.org/10.1111/ejn.13901\">https://doi.org/10.1111/ejn.13901</a>.","apa":"Sawada, K., Kawakami, R., Shigemoto, R., &#38; Nemoto, T. (2018). Super resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices. <i>European Journal of Neuroscience</i>. Wiley. <a href=\"https://doi.org/10.1111/ejn.13901\">https://doi.org/10.1111/ejn.13901</a>","ista":"Sawada K, Kawakami R, Shigemoto R, Nemoto T. 2018. Super resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices. European Journal of Neuroscience. 47(9), 1033–1042.","ieee":"K. Sawada, R. Kawakami, R. Shigemoto, and T. Nemoto, “Super resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices,” <i>European Journal of Neuroscience</i>, vol. 47, no. 9. Wiley, pp. 1033–1042, 2018.","short":"K. Sawada, R. Kawakami, R. Shigemoto, T. Nemoto, European Journal of Neuroscience 47 (2018) 1033–1042.","ama":"Sawada K, Kawakami R, Shigemoto R, Nemoto T. Super resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices. <i>European Journal of Neuroscience</i>. 2018;47(9):1033-1042. doi:<a href=\"https://doi.org/10.1111/ejn.13901\">10.1111/ejn.13901</a>"},"acknowledged_ssus":[{"_id":"EM-Fac"}],"title":"Super resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices","isi":1,"oa_version":"Published Version","scopus_import":"1","_id":"326","issue":"9","article_processing_charge":"No","license":"https://creativecommons.org/licenses/by-nc/4.0/","author":[{"first_name":"Kazuaki","full_name":"Sawada, Kazuaki","last_name":"Sawada"},{"full_name":"Kawakami, Ryosuke","last_name":"Kawakami","first_name":"Ryosuke"},{"first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi"},{"first_name":"Tomomi","last_name":"Nemoto","full_name":"Nemoto, Tomomi"}],"language":[{"iso":"eng"}],"oa":1,"page":"1033 - 1042","status":"public","day":"07","type":"journal_article","publisher":"Wiley","quality_controlled":"1","month":"03","file_date_updated":"2020-07-14T12:46:06Z","file":[{"file_name":"2018_EJN_Sawada.pdf","file_size":4850261,"date_updated":"2020-07-14T12:46:06Z","access_level":"open_access","creator":"dernst","content_type":"application/pdf","date_created":"2018-12-17T16:16:50Z","relation":"main_file","checksum":"98e901d8229e44aa8f3b51d248dedd09","file_id":"5721"}],"publication":"European Journal of Neuroscience","intvolume":"        47","department":[{"_id":"RySh"}],"external_id":{"isi":["000431496400001"]},"publication_status":"published","doi":"10.1111/ejn.13901","abstract":[{"lang":"eng","text":"Three-dimensional (3D) super-resolution microscopy technique structured illumination microscopy (SIM) imaging of dendritic spines along the dendrite has not been previously performed in fixed tissues, mainly due to deterioration of the stripe pattern of the excitation laser induced by light scattering and optical aberrations. To address this issue and solve these optical problems, we applied a novel clearing reagent, LUCID, to fixed brains. In SIM imaging, the penetration depth and the spatial resolution were improved in LUCID-treated slices, and 160-nm spatial resolution was obtained in a large portion of the imaging volume on a single apical dendrite. Furthermore, in a morphological analysis of spine heads of layer V pyramidal neurons (L5PNs) in the medial prefrontal cortex (mPFC) of chronic dexamethasone (Dex)-treated mice, SIM imaging revealed an altered distribution of spine forms that could not be detected by high-NA confocal imaging. Thus, super-resolution SIM imaging represents a promising high-throughput method for revealing spine morphologies in single dendrites."}],"date_published":"2018-03-07T00:00:00Z","ddc":["570"],"date_updated":"2023-09-19T09:58:40Z","volume":47,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)"},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_created":"2018-12-11T11:45:50Z","publist_id":"7539","year":"2018"},{"date_published":"2018-09-19T00:00:00Z","ddc":["570"],"project":[{"call_identifier":"H2020","grant_number":"720270","_id":"25CBA828-B435-11E9-9278-68D0E5697425","name":"Human Brain Project Specific Grant Agreement 1"}],"date_updated":"2025-07-10T11:52:35Z","volume":12,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2018-12-11T11:44:19Z","publist_id":"8013","year":"2018","file_date_updated":"2020-07-14T12:46:23Z","publication":"Frontiers in Cellular Neuroscience","intvolume":"        12","file":[{"relation":"main_file","checksum":"0bcaec8d596162af0b7fe3f31325d480","file_id":"5684","content_type":"application/pdf","date_created":"2018-12-17T08:49:03Z","creator":"dernst","file_name":"fncel-12-00311.pdf","file_size":6834251,"date_updated":"2020-07-14T12:46:23Z","access_level":"open_access"}],"department":[{"_id":"RySh"}],"external_id":{"isi":["000445090100002"]},"publication_status":"published","doi":"10.3389/fncel.2018.00311","ec_funded":1,"abstract":[{"text":"The small-conductance, Ca2+-activated K+ (SK) channel subtype SK2 regulates the spike rate and firing frequency, as well as Ca2+ transients in Purkinje cells (PCs). To understand the molecular basis by which SK2 channels mediate these functions, we analyzed the exact location and densities of SK2 channels along the neuronal surface of the mouse cerebellar PCs using SDS-digested freeze-fracture replica labeling (SDS-FRL) of high sensitivity combined with quantitative analyses. Immunogold particles for SK2 were observed on post- and pre-synaptic compartments showing both scattered and clustered distribution patterns. We found an axo-somato-dendritic gradient of the SK2 particle density increasing 12-fold from soma to dendritic spines. Using two different immunogold approaches, we also found that SK2 immunoparticles were frequently adjacent to, but never overlap with, the postsynaptic density of excitatory synapses in PC spines. Co-immunoprecipitation analysis demonstrated that SK2 channels form macromolecular complexes with two types of proteins that mobilize Ca2+: CaV2.1 channels and mGlu1α receptors in the cerebellum. Freeze-fracture replica double-labeling showed significant co-clustering of particles for SK2 with those for CaV2.1 channels and mGlu1α receptors. SK2 channels were also detected at presynaptic sites, mostly at the presynaptic active zone (AZ), where they are close to CaV2.1 channels, though they are not significantly co-clustered. These data demonstrate that SK2 channels located in different neuronal compartments can associate with distinct proteins mobilizing Ca2+, and suggest that the ultrastructural association of SK2 with CaV2.1 and mGlu1α provides the mechanism that ensures voltage (excitability) regulation by distinct intracellular Ca2+ transients in PCs.","lang":"eng"}],"_id":"41","article_processing_charge":"No","article_type":"original","author":[{"last_name":"Luján","full_name":"Luján, Rafæl","first_name":"Rafæl"},{"first_name":"Carolina","last_name":"Aguado","full_name":"Aguado, Carolina"},{"first_name":"Francisco","last_name":"Ciruela","full_name":"Ciruela, Francisco"},{"first_name":"Xavier","last_name":"Arus","full_name":"Arus, Xavier"},{"full_name":"Martín Belmonte, Alejandro","last_name":"Martín Belmonte","first_name":"Alejandro"},{"first_name":"Rocío","full_name":"Alfaro Ruiz, Rocío","last_name":"Alfaro Ruiz"},{"first_name":"Jesus","full_name":"Martinez Gomez, Jesus","last_name":"Martinez Gomez"},{"full_name":"De La Ossa, Luis","last_name":"De La Ossa","first_name":"Luis"},{"full_name":"Watanabe, Masahiko","last_name":"Watanabe","first_name":"Masahiko"},{"first_name":"John","full_name":"Adelman, John","last_name":"Adelman"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","first_name":"Ryuichi"},{"first_name":"Yugo","full_name":"Fukazawa, Yugo","last_name":"Fukazawa"}],"language":[{"iso":"eng"}],"oa":1,"status":"public","day":"19","type":"journal_article","article_number":"311","publisher":"Frontiers Media","publication_identifier":{"issn":["1662-5102"]},"month":"09","quality_controlled":"1","has_accepted_license":"1","citation":{"ieee":"R. Luján <i>et al.</i>, “Sk2 channels associate with mGlu1α receptors and CaV2.1 channels in Purkinje cells,” <i>Frontiers in Cellular Neuroscience</i>, vol. 12. Frontiers Media, 2018.","ama":"Luján R, Aguado C, Ciruela F, et al. Sk2 channels associate with mGlu1α receptors and CaV2.1 channels in Purkinje cells. <i>Frontiers in Cellular Neuroscience</i>. 2018;12. doi:<a href=\"https://doi.org/10.3389/fncel.2018.00311\">10.3389/fncel.2018.00311</a>","short":"R. Luján, C. Aguado, F. Ciruela, X. Arus, A. Martín Belmonte, R. Alfaro Ruiz, J. Martinez Gomez, L. De La Ossa, M. Watanabe, J. Adelman, R. Shigemoto, Y. Fukazawa, Frontiers in Cellular Neuroscience 12 (2018).","ista":"Luján R, Aguado C, Ciruela F, Arus X, Martín Belmonte A, Alfaro Ruiz R, Martinez Gomez J, De La Ossa L, Watanabe M, Adelman J, Shigemoto R, Fukazawa Y. 2018. Sk2 channels associate with mGlu1α receptors and CaV2.1 channels in Purkinje cells. Frontiers in Cellular Neuroscience. 12, 311.","apa":"Luján, R., Aguado, C., Ciruela, F., Arus, X., Martín Belmonte, A., Alfaro Ruiz, R., … Fukazawa, Y. (2018). Sk2 channels associate with mGlu1α receptors and CaV2.1 channels in Purkinje cells. <i>Frontiers in Cellular Neuroscience</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fncel.2018.00311\">https://doi.org/10.3389/fncel.2018.00311</a>","mla":"Luján, Rafæl, et al. “Sk2 Channels Associate with MGlu1α Receptors and CaV2.1 Channels in Purkinje Cells.” <i>Frontiers in Cellular Neuroscience</i>, vol. 12, 311, Frontiers Media, 2018, doi:<a href=\"https://doi.org/10.3389/fncel.2018.00311\">10.3389/fncel.2018.00311</a>.","chicago":"Luján, Rafæl, Carolina Aguado, Francisco Ciruela, Xavier Arus, Alejandro Martín Belmonte, Rocío Alfaro Ruiz, Jesus Martinez Gomez, et al. “Sk2 Channels Associate with MGlu1α Receptors and CaV2.1 Channels in Purkinje Cells.” <i>Frontiers in Cellular Neuroscience</i>. Frontiers Media, 2018. <a href=\"https://doi.org/10.3389/fncel.2018.00311\">https://doi.org/10.3389/fncel.2018.00311</a>."},"title":"Sk2 channels associate with mGlu1α receptors and CaV2.1 channels in Purkinje cells","isi":1,"scopus_import":"1","oa_version":"Published Version"},{"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"volume":223,"date_updated":"2026-08-09T22:31:00Z","project":[{"call_identifier":"H2020","_id":"25CBA828-B435-11E9-9278-68D0E5697425","grant_number":"720270","name":"Human Brain Project Specific Grant Agreement 1"},{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425"}],"ddc":["571"],"date_published":"2018-04-01T00:00:00Z","year":"2018","publist_id":"7192","date_created":"2018-12-11T11:47:29Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file":[{"creator":"system","date_updated":"2020-07-14T12:47:20Z","file_size":5542926,"file_name":"IST-2018-1013-v1+1_2018_Kleindienst_Differential.pdf","access_level":"open_access","relation":"main_file","checksum":"a55b3103476ecb5f4f983d8801807e8b","file_id":"5157","content_type":"application/pdf","date_created":"2018-12-12T10:15:36Z"}],"intvolume":"       223","publication":"Brain Structure and Function","file_date_updated":"2020-07-14T12:47:20Z","ec_funded":1,"abstract":[{"lang":"eng","text":"Metabotropic GABAB receptors mediate slow inhibitory effects presynaptically and postsynaptically through the modulation of different effector signalling pathways. Here, we analysed the distribution of GABAB receptors using highly sensitive SDS-digested freeze-fracture replica labelling in mouse cerebellar Purkinje cells. Immunoreactivity for GABAB1 was observed on presynaptic and, more abundantly, on postsynaptic compartments, showing both scattered and clustered distribution patterns. Quantitative analysis of immunoparticles revealed a somato-dendritic gradient, with the density of immunoparticles increasing 26-fold from somata to dendritic spines. To understand the spatial relationship of GABAB receptors with two key effector ion channels, the G protein-gated inwardly rectifying K+ (GIRK/Kir3) channel and the voltage-dependent Ca2+ channel, biochemical and immunohistochemical approaches were performed. Co-immunoprecipitation analysis demonstrated that GABAB receptors co-assembled with GIRK and CaV2.1 channels in the cerebellum. Using double-labelling immunoelectron microscopic techniques, co-clustering between GABAB1 and GIRK2 was detected in dendritic spines, whereas they were mainly segregated in the dendritic shafts. In contrast, co-clustering of GABAB1 and CaV2.1 was detected in dendritic shafts but not spines. Presynaptically, although no significant co-clustering of GABAB1 and GIRK2 or CaV2.1 channels was detected, inter-cluster distance for GABAB1 and GIRK2 was significantly smaller in the active zone than in the dendritic shafts, and that for GABAB1 and CaV2.1 was significantly smaller in the active zone than in the dendritic shafts and spines. Thus, GABAB receptors are associated with GIRK and CaV2.1 channels in different subcellular compartments. These data provide a better framework for understanding the different roles played by GABAB receptors and their effector ion channels in the cerebellar network."}],"doi":"10.1007/s00429-017-1568-y","publication_status":"published","external_id":{"isi":["000428419500030"]},"department":[{"_id":"RySh"}],"language":[{"iso":"eng"}],"oa":1,"author":[{"first_name":"Rafael","last_name":"Luján","full_name":"Luján, Rafael"},{"last_name":"Aguado","full_name":"Aguado, Carolina","first_name":"Carolina"},{"full_name":"Ciruela, Francisco","last_name":"Ciruela","first_name":"Francisco"},{"first_name":"Javier","last_name":"Cózar","full_name":"Cózar, Javier"},{"first_name":"David","id":"42E121A4-F248-11E8-B48F-1D18A9856A87","full_name":"Kleindienst, David","last_name":"Kleindienst"},{"first_name":"Luis","full_name":"De La Ossa, Luis","last_name":"De La Ossa"},{"first_name":"Bernhard","last_name":"Bettler","full_name":"Bettler, Bernhard"},{"full_name":"Wickman, Kevin","last_name":"Wickman","first_name":"Kevin"},{"first_name":"Masahiko","full_name":"Watanabe, Masahiko","last_name":"Watanabe"},{"last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi"},{"first_name":"Yugo","last_name":"Fukazawa","full_name":"Fukazawa, Yugo"}],"article_type":"original","article_processing_charge":"No","issue":"3","_id":"612","quality_controlled":"1","month":"04","publisher":"Springer","type":"journal_article","day":"01","status":"public","page":"1565 - 1587","citation":{"chicago":"Luján, Rafael, Carolina Aguado, Francisco Ciruela, Javier Cózar, David Kleindienst, Luis De La Ossa, Bernhard Bettler, et al. “Differential Association of GABAB Receptors with Their Effector Ion Channels in Purkinje Cells.” <i>Brain Structure and Function</i>. Springer, 2018. <a href=\"https://doi.org/10.1007/s00429-017-1568-y\">https://doi.org/10.1007/s00429-017-1568-y</a>.","mla":"Luján, Rafael, et al. “Differential Association of GABAB Receptors with Their Effector Ion Channels in Purkinje Cells.” <i>Brain Structure and Function</i>, vol. 223, no. 3, Springer, 2018, pp. 1565–87, doi:<a href=\"https://doi.org/10.1007/s00429-017-1568-y\">10.1007/s00429-017-1568-y</a>.","apa":"Luján, R., Aguado, C., Ciruela, F., Cózar, J., Kleindienst, D., De La Ossa, L., … Fukazawa, Y. (2018). Differential association of GABAB receptors with their effector ion channels in Purkinje cells. <i>Brain Structure and Function</i>. Springer. <a href=\"https://doi.org/10.1007/s00429-017-1568-y\">https://doi.org/10.1007/s00429-017-1568-y</a>","ista":"Luján R, Aguado C, Ciruela F, Cózar J, Kleindienst D, De La Ossa L, Bettler B, Wickman K, Watanabe M, Shigemoto R, Fukazawa Y. 2018. Differential association of GABAB receptors with their effector ion channels in Purkinje cells. Brain Structure and Function. 223(3), 1565–1587.","ieee":"R. Luján <i>et al.</i>, “Differential association of GABAB receptors with their effector ion channels in Purkinje cells,” <i>Brain Structure and Function</i>, vol. 223, no. 3. Springer, pp. 1565–1587, 2018.","short":"R. Luján, C. Aguado, F. Ciruela, J. Cózar, D. Kleindienst, L. De La Ossa, B. Bettler, K. Wickman, M. Watanabe, R. Shigemoto, Y. Fukazawa, Brain Structure and Function 223 (2018) 1565–1587.","ama":"Luján R, Aguado C, Ciruela F, et al. Differential association of GABAB receptors with their effector ion channels in Purkinje cells. <i>Brain Structure and Function</i>. 2018;223(3):1565-1587. doi:<a href=\"https://doi.org/10.1007/s00429-017-1568-y\">10.1007/s00429-017-1568-y</a>"},"has_accepted_license":"1","pubrep_id":"1013","scopus_import":"1","oa_version":"Published Version","isi":1,"title":"Differential association of GABAB receptors with their effector ion channels in Purkinje cells","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"9562"}]}},{"ddc":["571","576"],"date_published":"2018-06-27T00:00:00Z","date_updated":"2026-07-31T09:40:17Z","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","year":"2018","publist_id":"8003","date_created":"2018-12-11T11:44:22Z","file_date_updated":"2021-02-11T23:30:13Z","file":[{"content_type":"application/msword","date_created":"2019-04-09T07:16:26Z","relation":"source_file","file_id":"6251","checksum":"dcc7b55619d8509dd62b8e99d6cdee44","date_updated":"2021-02-11T23:30:13Z","file_size":141270528,"file_name":"2018_Thesis_Case_Source.doc","embargo_to":"open_access","access_level":"closed","creator":"dernst"},{"relation":"main_file","file_id":"6252","checksum":"f69fdd5c8709c4e618aa8c1a1221153d","content_type":"application/pdf","date_created":"2019-04-09T07:16:23Z","creator":"dernst","embargo":"2019-07-05","file_size":15193621,"file_name":"2018_Thesis_Case.pdf","date_updated":"2021-02-11T11:17:14Z","access_level":"open_access"}],"doi_confirm":"1","publication_status":"published","department":[{"_id":"RySh"},{"_id":"GradSch"}],"abstract":[{"text":"Asymmetries have long been known about in the central nervous system. From gross anatomical differences, such as the presence of the parapineal organ in only one hemisphere of the developing zebrafish, to more subtle differences in activity between both hemispheres, as seen in freely roaming animals or human participants under PET and fMRI imaging analysis. The presence of asymmetries has been demonstrated to have huge behavioural implications, with their disruption often leading to the generation of neurological disorders, memory problems, changes in personality, and in an organism's health and well-being. For my Ph.D. work I aimed to tackle two important avenues of research. The first being the process of input-side dependency in the hippocampus, with the goal of finding a key gene responsible for its development (Gene X). The second project was to do with experience-induced laterality formation in the hippocampus. Specifically, how laterality in the synapse density of the CA1 stratum radiatum (s.r.) could be induced purely through environmental enrichment. Through unilateral tracer injections into the CA3, I was able to selectively measure the properties of synapses within the CA1 and investigate how they differed based upon which hemisphere the presynaptic neurone originated. Having found the existence of a previously unreported reversed (left-isomerism) i.v. mutant, through morpholocal examination of labelled terminals in the CA1 s.r., I aimed to elucidate a key gene responsible for the process of left or right determination of inputs to the CA1 s.r.. This work relates to the previous finding of input-side dependent asymmetry in the wild-type rodent, where the origin of the projecting neurone to the CA1 will determine the morphology of a synapse, to a greater degree than the hemisphere in which the projection terminates. Using left- and right-isomerism i.v. mice, in combination with whole genome sequence analysis, I highlight Ena/VASP-like (Evl) as a potential target for Gene X. In relation to this topic, I also highlight my work in the recently published paper of how knockout of PirB can lead to a lack of input-side dependency in the murine hippocampus. For the second question, I show that the environmental enrichment paradigm will lead to an asymmetry in the synapse densities in the hippocampus of mice. I also highlight that the nature of the enrichment is of less consequence than the process of enrichment itself. I demonstrate that the CA3 region will dramatically alter its projection targets, in relation to environmental stimulation, with the asymmetry in synaptic density, caused by enrichment, relying heavily on commissural fibres. I also highlight the vital importance of input-side dependent asymmetry, as a necessary component of experience-dependent laterality formation in the CA1 s.r.. However, my results suggest that it isn't the only cause, as there appears to be a CA1 dependent mechanism also at play. Upon further investigation, I highlight the significant, and highly important, finding that the changes seen in the CA1 s.r. were predominantly caused through projections from the left-CA3, with the right-CA3 having less involvement in this mechanism.","lang":"eng"}],"alternative_title":["ISTA Thesis"],"doi":"10.15479/AT:ISTA:th_1032","degree_awarded":"PhD","article_processing_charge":"No","corr_author":"1","_id":"51","oa":1,"language":[{"iso":"eng"}],"supervisor":[{"first_name":"Ryuichi","last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"}],"author":[{"last_name":"Case","full_name":"Case, Matthew J","id":"44B7CA5A-F248-11E8-B48F-1D18A9856A87","first_name":"Matthew J"}],"type":"dissertation","day":"27","status":"public","page":"186","OA_place":"publisher","month":"06","publication_identifier":{"issn":["2663-337X"]},"publisher":"Institute of Science and Technology Austria","citation":{"mla":"Case, Matthew J. <i>From the Left to the Right: A Tale of Asymmetries, Environments, and Hippocampal Development</i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_1032\">10.15479/AT:ISTA:th_1032</a>.","chicago":"Case, Matthew J. “From the Left to the Right: A Tale of Asymmetries, Environments, and Hippocampal Development.” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:th_1032\">https://doi.org/10.15479/AT:ISTA:th_1032</a>.","ista":"Case MJ. 2018. From the left to the right: A tale of asymmetries, environments, and hippocampal development. Institute of Science and Technology Austria.","apa":"Case, M. J. (2018). <i>From the left to the right: A tale of asymmetries, environments, and hippocampal development</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th_1032\">https://doi.org/10.15479/AT:ISTA:th_1032</a>","short":"M.J. Case, From the Left to the Right: A Tale of Asymmetries, Environments, and Hippocampal Development, Institute of Science and Technology Austria, 2018.","ama":"Case MJ. From the left to the right: A tale of asymmetries, environments, and hippocampal development. 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_1032\">10.15479/AT:ISTA:th_1032</a>","ieee":"M. J. Case, “From the left to the right: A tale of asymmetries, environments, and hippocampal development,” Institute of Science and Technology Austria, 2018."},"has_accepted_license":"1","pubrep_id":"1032","title":"From the left to the right: A tale of asymmetries, environments, and hippocampal development","related_material":{"record":[{"id":"682","relation":"part_of_dissertation","status":"public"}]},"oa_version":"Published Version"},{"article_processing_charge":"No","_id":"1146","issue":"4","language":[{"iso":"eng"}],"author":[{"first_name":"Wuping","last_name":"Sun","full_name":"Sun, Wuping"},{"first_name":"Ming-Zhu","id":"34009CFA-F248-11E8-B48F-1D18A9856A87","full_name":"Zhai, Ming-Zhu","last_name":"Zhai"},{"last_name":"Li","full_name":"Li, Da","first_name":"Da"},{"first_name":"Yiming","full_name":"Zhou, Yiming","last_name":"Zhou"},{"first_name":"Nana","full_name":"Chen, Nana","last_name":"Chen"},{"first_name":"Ming","full_name":"Guo, Ming","last_name":"Guo"},{"full_name":"Zhou, Shisheng","last_name":"Zhou","first_name":"Shisheng"}],"type":"journal_article","day":"01","page":"1136-1142","status":"public","quality_controlled":"1","month":"08","publisher":"Elsevier","publication_identifier":{"issn":["0261-5614"]},"citation":{"mla":"Sun, Wuping, et al. “Comparison of the Effects of Nicotinic Acid and Nicotinamide Degradation on Plasma Betaine and Choline Levels.” <i>Clinical Nutrition</i>, vol. 36, no. 4, Elsevier, 2017, pp. 1136–42, doi:<a href=\"https://doi.org/10.1016/j.clnu.2016.07.016\">10.1016/j.clnu.2016.07.016</a>.","chicago":"Sun, Wuping, Ming-Zhu Zhai, Da Li, Yiming Zhou, Nana Chen, Ming Guo, and Shisheng Zhou. “Comparison of the Effects of Nicotinic Acid and Nicotinamide Degradation on Plasma Betaine and Choline Levels.” <i>Clinical Nutrition</i>. Elsevier, 2017. <a href=\"https://doi.org/10.1016/j.clnu.2016.07.016\">https://doi.org/10.1016/j.clnu.2016.07.016</a>.","ama":"Sun W, Zhai M-Z, Li D, et al. Comparison of the effects of nicotinic acid and nicotinamide degradation on plasma betaine and choline levels. <i>Clinical Nutrition</i>. 2017;36(4):1136-1142. doi:<a href=\"https://doi.org/10.1016/j.clnu.2016.07.016\">10.1016/j.clnu.2016.07.016</a>","ieee":"W. Sun <i>et al.</i>, “Comparison of the effects of nicotinic acid and nicotinamide degradation on plasma betaine and choline levels,” <i>Clinical Nutrition</i>, vol. 36, no. 4. Elsevier, pp. 1136–1142, 2017.","short":"W. Sun, M.-Z. Zhai, D. Li, Y. Zhou, N. Chen, M. Guo, S. Zhou, Clinical Nutrition 36 (2017) 1136–1142.","ista":"Sun W, Zhai M-Z, Li D, Zhou Y, Chen N, Guo M, Zhou S. 2017. Comparison of the effects of nicotinic acid and nicotinamide degradation on plasma betaine and choline levels. Clinical Nutrition. 36(4), 1136–1142.","apa":"Sun, W., Zhai, M.-Z., Li, D., Zhou, Y., Chen, N., Guo, M., &#38; Zhou, S. (2017). Comparison of the effects of nicotinic acid and nicotinamide degradation on plasma betaine and choline levels. <i>Clinical Nutrition</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.clnu.2016.07.016\">https://doi.org/10.1016/j.clnu.2016.07.016</a>"},"title":"Comparison of the effects of nicotinic acid and nicotinamide degradation on plasma betaine and choline levels","acknowledgement":"We thank all the participants for their contribution to this study and volunteers from the Nursing School of Dalian University for their supporting to collect blood and urine samples of the participants. We also thank Dr. Yasunori Takayama from National Institute for Physiological Sciences of Japan for his kind help.","oa_version":"None","scopus_import":"1","isi":1,"date_published":"2017-08-01T00:00:00Z","volume":36,"date_updated":"2025-09-22T09:59:49Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2017","publist_id":"6212","date_created":"2018-12-11T11:50:24Z","intvolume":"        36","publication":"Clinical Nutrition","publication_status":"published","external_id":{"isi":["000404315200029"]},"department":[{"_id":"RySh"}],"abstract":[{"text":"Aim: The present study was to compare the effects of nicotinic acid and nicotinamide on the plasma methyl donors, choline and betaine. Methods: Thirty adult subjects were randomly divided into three groups of equal size, and orally received purified water (C group), nicotinic acid (300 mg, NA group) or nicotinamide (300 mg, NM group). Plasma nicotinamide, N 1-methylnicotinamide, homocysteine, betaine and choline levels before and 1.5-h and 3-h post-dosing, plasma normetanephrine and metanephrine concentrations at 3-h post-dosing, and the urinary excretion of N 1-methyl-2-pyridone-5-carboxamide during the test period were examined. Results: The level of 3-h plasma nicotinamide, N 1-methylnicotinamide, homocysteine, the urinary excretion of N 1-methyl-2-pyridone-5-carboxamide and pulse pressure (PP) in the NM group was 221%, 3972%, 61%, 1728% and 21.2% higher than that of the control group (P &lt; 0.01, except homocysteine and PP P &lt; 0.05), while the 3-h plasma betaine, normetanephrine and metanephrine level in the NM group was 24.4%, 9.4% and 11.7% lower (P &lt; 0.05, except betaine P &lt; 0.01), without significant difference in choline levels. Similar but less pronounced changes were observed in the NA group, with a lower level of 3-h plasma N 1-methylnicotinamide (1.90 ± 0.20 μmol/l vs. 3.62 ± 0.27 μmol/l, P &lt; 0.01) and homocysteine (12.85 ± 1.39 μmol/l vs. 18.08 ± 1.02 μmol/l, P &lt; 0.05) but a higher level of betaine (27.44 ± 0.71 μmol/l vs. 23.52 ± 0.61 μmol/l, P &lt; 0.05) than that of the NM group. Conclusion: The degradation of nicotinamide consumes more betaine than that of nicotinic acid at identical doses. This difference should be taken into consideration in niacin fortification. © 2016 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism.","lang":"eng"}],"doi":"10.1016/j.clnu.2016.07.016"},{"date_updated":"2025-09-11T07:28:59Z","volume":8,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"date_published":"2017-08-24T00:00:00Z","ddc":["571"],"date_created":"2018-12-11T11:47:34Z","publist_id":"7167","year":"2017","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","file":[{"file_name":"IST-2017-907-v1+1_20540-294640-4-PB.pdf","file_size":6101606,"date_updated":"2020-07-14T12:47:26Z","access_level":"open_access","creator":"system","content_type":"application/pdf","date_created":"2018-12-12T10:16:15Z","relation":"main_file","file_id":"5201","checksum":"2219e5348bbfe1aac2725aa620c33280"}],"intvolume":"         8","publication":"Oncotarget","file_date_updated":"2020-07-14T12:47:26Z","doi":"10.18632/oncotarget.20540","abstract":[{"text":"Beige adipocytes are a new type of recruitable brownish adipocytes, with highly mitochondrial membrane uncoupling protein 1 expression and thermogenesis. Beige adipocytes were found among white adipocytes, especially in subcutaneous white adipose tissue (sWAT). Therefore, beige adipocytes may be involved in the regulation of energy metabolism and fat deposition. Transient receptor potential melastatin 8 (TRPM8), a Ca2+-permeable non-selective cation channel, plays vital roles in the regulation of various cellular functions. It has been reported that TRPM8 activation enhanced the thermogenic function of brown adiposytes. However, the involvement of TRPM8 in the thermogenic function of WAT remains unexplored. Our data revealed that TRPM8 was expressed in mouse white adipocytes at mRNA, protein and functional levels. The mRNA expression of Trpm8 was significantly increased in the differentiated white adipocytes than pre-adipocytes. Moreover, activation of TRPM8 by menthol enhanced the expression of thermogenic genes in cultured white aidpocytes. And menthol-induced increases of the thermogenic genes in white adipocytes was inhibited by either KT5720 (a protein kinase A inhibitor) or BAPTA-AM. In addition, high fat diet (HFD)-induced obesity in mice was significantly recovered by co-treatment with menthol. Dietary menthol enhanced WAT &quot;browning&quot; and improved glucose metabolism in HFD-induced obesity mice as well. Therefore, we concluded that TRPM8 might be involved in WAT &quot;browning&quot; by increasing the expression levels of genes related to thermogenesis and energy metabolism. And dietary menthol could be a novel approach for combating human obesity and related metabolic diseases.","lang":"eng"}],"department":[{"_id":"RySh"}],"external_id":{"isi":["000411760400142"]},"publication_status":"published","author":[{"first_name":"Changyu","last_name":"Jiang","full_name":"Jiang, Changyu"},{"first_name":"Ming-Zhu","last_name":"Zhai","full_name":"Zhai, Ming-Zhu","id":"34009CFA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Yan, Dong","last_name":"Yan","first_name":"Dong"},{"last_name":"Li","full_name":"Li, Da","first_name":"Da"},{"first_name":"Chen","full_name":"Li, Chen","last_name":"Li"},{"first_name":"Yonghong","full_name":"Zhang, Yonghong","last_name":"Zhang"},{"full_name":"Xiao, Lizu","last_name":"Xiao","first_name":"Lizu"},{"first_name":"Donglin","last_name":"Xiong","full_name":"Xiong, Donglin"},{"last_name":"Deng","full_name":"Deng, Qiwen","first_name":"Qiwen"},{"full_name":"Sun, Wuping","last_name":"Sun","first_name":"Wuping"}],"oa":1,"language":[{"iso":"eng"}],"issue":"43","_id":"627","article_processing_charge":"No","publication_identifier":{"issn":["1949-2553"]},"publisher":"Impact Journals","month":"08","quality_controlled":"1","page":"75114 - 75126","status":"public","day":"24","type":"journal_article","pubrep_id":"907","has_accepted_license":"1","citation":{"mla":"Jiang, Changyu, et al. “Dietary Menthol-Induced TRPM8 Activation Enhances WAT ‘Browning’ and Ameliorates Diet-Induced Obesity.” <i>Oncotarget</i>, vol. 8, no. 43, Impact Journals, 2017, pp. 75114–26, doi:<a href=\"https://doi.org/10.18632/oncotarget.20540\">10.18632/oncotarget.20540</a>.","chicago":"Jiang, Changyu, Ming-Zhu Zhai, Dong Yan, Da Li, Chen Li, Yonghong Zhang, Lizu Xiao, Donglin Xiong, Qiwen Deng, and Wuping Sun. “Dietary Menthol-Induced TRPM8 Activation Enhances WAT ‘Browning’ and Ameliorates Diet-Induced Obesity.” <i>Oncotarget</i>. Impact Journals, 2017. <a href=\"https://doi.org/10.18632/oncotarget.20540\">https://doi.org/10.18632/oncotarget.20540</a>.","apa":"Jiang, C., Zhai, M.-Z., Yan, D., Li, D., Li, C., Zhang, Y., … Sun, W. (2017). Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity. <i>Oncotarget</i>. Impact Journals. <a href=\"https://doi.org/10.18632/oncotarget.20540\">https://doi.org/10.18632/oncotarget.20540</a>","ista":"Jiang C, Zhai M-Z, Yan D, Li D, Li C, Zhang Y, Xiao L, Xiong D, Deng Q, Sun W. 2017. Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity. Oncotarget. 8(43), 75114–75126.","ieee":"C. Jiang <i>et al.</i>, “Dietary menthol-induced TRPM8 activation enhances WAT ‘browning’ and ameliorates diet-induced obesity,” <i>Oncotarget</i>, vol. 8, no. 43. Impact Journals, pp. 75114–75126, 2017.","short":"C. Jiang, M.-Z. Zhai, D. Yan, D. Li, C. Li, Y. Zhang, L. Xiao, D. Xiong, Q. Deng, W. Sun, Oncotarget 8 (2017) 75114–75126.","ama":"Jiang C, Zhai M-Z, Yan D, et al. Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity. <i>Oncotarget</i>. 2017;8(43):75114-75126. doi:<a href=\"https://doi.org/10.18632/oncotarget.20540\">10.18632/oncotarget.20540</a>"},"isi":1,"scopus_import":"1","oa_version":"Published Version","title":"Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity"}]