[{"_id":"21231","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"NanoFab"}],"pmid":1,"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","day":"05","OA_place":"publisher","publication":"npj Systems Biology and Applications","year":"2026","DOAJ_listed":"1","date_created":"2026-02-16T10:44:31Z","article_type":"original","file_date_updated":"2026-02-23T10:09:03Z","ddc":["570"],"language":[{"iso":"eng"}],"author":[{"first_name":"Jonas","last_name":"Arruda","full_name":"Arruda, Jonas"},{"first_name":"Emad","last_name":"Alamoudi","full_name":"Alamoudi, Emad"},{"full_name":"Mueller, Robert","last_name":"Mueller","first_name":"Robert"},{"first_name":"Marc","last_name":"Vaisband","full_name":"Vaisband, Marc"},{"last_name":"Molkenbur","first_name":"Ronja","full_name":"Molkenbur, Ronja"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","first_name":"Jack","last_name":"Merrin","orcid":"0000-0001-5145-4609"},{"full_name":"Kiermaier, Eva","last_name":"Kiermaier","first_name":"Eva"},{"full_name":"Hasenauer, Jan","first_name":"Jan","last_name":"Hasenauer"}],"oa":1,"publication_status":"published","doi":"10.1038/s41540-026-00648-9","file":[{"content_type":"application/pdf","date_updated":"2026-02-23T10:09:03Z","relation":"main_file","file_size":10217687,"access_level":"open_access","file_name":"2026_npjSysBioApp_Arruda.pdf","checksum":"99b2e6bbaaedf45f22e07751948669f5","creator":"dernst","file_id":"21346","success":1,"date_created":"2026-02-23T10:09:03Z"}],"intvolume":"        12","article_number":"20","citation":{"ama":"Arruda J, Alamoudi E, Mueller R, et al. Simulation-based inference of cell migration dynamics in complex spatial environments. <i>npj Systems Biology and Applications</i>. 2026;12. doi:<a href=\"https://doi.org/10.1038/s41540-026-00648-9\">10.1038/s41540-026-00648-9</a>","ieee":"J. Arruda <i>et al.</i>, “Simulation-based inference of cell migration dynamics in complex spatial environments,” <i>npj Systems Biology and Applications</i>, vol. 12. Springer Nature, 2026.","short":"J. Arruda, E. Alamoudi, R. Mueller, M. Vaisband, R. Molkenbur, J. Merrin, E. Kiermaier, J. Hasenauer, Npj Systems Biology and Applications 12 (2026).","ista":"Arruda J, Alamoudi E, Mueller R, Vaisband M, Molkenbur R, Merrin J, Kiermaier E, Hasenauer J. 2026. Simulation-based inference of cell migration dynamics in complex spatial environments. npj Systems Biology and Applications. 12, 20.","mla":"Arruda, Jonas, et al. “Simulation-Based Inference of Cell Migration Dynamics in Complex Spatial Environments.” <i>Npj Systems Biology and Applications</i>, vol. 12, 20, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41540-026-00648-9\">10.1038/s41540-026-00648-9</a>.","chicago":"Arruda, Jonas, Emad Alamoudi, Robert Mueller, Marc Vaisband, Ronja Molkenbur, Jack Merrin, Eva Kiermaier, and Jan Hasenauer. “Simulation-Based Inference of Cell Migration Dynamics in Complex Spatial Environments.” <i>Npj Systems Biology and Applications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41540-026-00648-9\">https://doi.org/10.1038/s41540-026-00648-9</a>.","apa":"Arruda, J., Alamoudi, E., Mueller, R., Vaisband, M., Molkenbur, R., Merrin, J., … Hasenauer, J. (2026). Simulation-based inference of cell migration dynamics in complex spatial environments. <i>Npj Systems Biology and Applications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41540-026-00648-9\">https://doi.org/10.1038/s41540-026-00648-9</a>"},"abstract":[{"text":"To assess cell migration in complex spatial environments, microfabricated chips, such as mazes and pillar forests, are routinely used to impose spatial and mechanical constraints, and cell trajectories are followed within these structures by advanced imaging techniques. In systems mechanobiology, computational models serve as essential tools to uncover how physical geometry influences intracellular dynamics; however, decoding such complex behaviors requires advanced inference techniques. Here, we integrated experimental observations of dendritic cell migration in a geometrically constrained microenvironment into a Cellular Potts model. We demonstrated that these spatial constraints modulate the motility dynamics, including speed and directional changes. We show that classical summary statistics, such as mean squared displacement and turning angle distributions, can resolve key mechanistic features but fail to extract richer spatiotemporal patterns, limiting accurate parameter inference. To solve this, we applied neural posterior estimation with in-the-loop learning of summary features. This learned summary representation of the data enables robust and flexible parameter inference, providing a data-driven framework for model calibration and advancing quantitative analysis of cell migration in structured microenvironments.","lang":"eng"}],"scopus_import":"1","oa_version":"Published Version","publisher":"Springer Nature","OA_type":"gold","type":"journal_article","publication_identifier":{"eissn":["2056-7189"]},"status":"public","volume":12,"PlanS_conform":"1","title":"Simulation-based inference of cell migration dynamics in complex spatial environments","article_processing_charge":"Yes (via OA deal)","date_published":"2026-02-05T00:00:00Z","acknowledgement":"This work was supported by the German Federal Ministry of Education and Research (BMBF) (EMUNE/031L0293C), the European Union via the ERC grant INTEGRATE, grant agreement number 101126146, and under Germany’s Excellence Strategy by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) (EXC 2047—390685813, EXC 2151—390873048, FOR5775 — 533863915, and 524747443), the University of Bonn via the Schlegel Professorship of J.H., and the returning experts fellowship of the Ministry of Innovation, Science, and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069). J.M. is a member of the Nanofabrication Facility and is supported by the Institute of Science and Technology Austria. E.K. acknowledges the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments. The authors thank Laeschkir Würthner for his insightful comments on the implementation of the authors’ model. The views and opinions expressed are those of the authors only and do not necessarily reflect those of the funding agencies. Parts of Fig. 1 were created using BioRender. Open Access funding enabled and organized by Projekt DEAL.","external_id":{"pmid":["41611727"]},"date_updated":"2026-02-23T10:10:10Z","quality_controlled":"1","month":"02"},{"date_updated":"2026-04-28T12:12:46Z","quality_controlled":"1","external_id":{"pmid":["41698893"]},"month":"02","volume":17,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"M-Shop"}],"date_published":"2026-02-16T00:00:00Z","article_processing_charge":"Yes","acknowledgement":"We are grateful to A. G. Volosniev for the valuable discussions. We thank D. Milius for the assistance with microscopy. D. R. would like to thank F. Filakovský and T. Čuchráč for the valuable discussions. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Miba Machine Shop Facility (MS).","title":"Flexoelectric domain walls enable charge separation and transport in cubic perovskites","PlanS_conform":"1","OA_type":"gold","related_material":{"link":[{"url":"https://ista.ac.at/en/news/explaining-next-generation-solar-cells/","description":"News on ISTA website","relation":"press_release"}]},"status":"public","type":"journal_article","publication_identifier":{"eissn":["2041-1723"]},"scopus_import":"1","abstract":[{"text":"The exceptional energy-harvesting efficiency of lead-halide perovskites arises from unusually long photocarrier diffusion lengths and recombination lifetimes that persist even in defect-rich, solution-grown samples. Paradoxically, perovskites are also known for having very short exciton decay times. Here, we resolve this apparent contradiction by showing that key optoelectronic properties of perovskites can be explained by localized flexoelectric polarization confined to interfaces between domains of spontaneous strain. Using birefringence imaging, electrochemical staining, and zero-bias photocurrent measurements, we visualize the domain structure and directly probe the associated internal fields in nominally cubic single crystals of methylammonium lead bromide. We demonstrate that localized flexoelectric fields spatially separate electrons and holes to opposite sides of domain walls, exponentially suppressing recombination. Domain walls thus act as efficient mesoscopic transport channels for long-lived photocarriers, microscopically linking structural heterogeneity to charge transport and offering mechanistically informed design principles for perovskite solar-energy technologies.","lang":"eng"}],"citation":{"ieee":"D. Rak, D. Lorenc, D. Balazs, A. A. Zhumekenov, O. M. Bakr, and Z. Alpichshev, “Flexoelectric domain walls enable charge separation and transport in cubic perovskites,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026.","ama":"Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. Flexoelectric domain walls enable charge separation and transport in cubic perovskites. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-68660-5\">10.1038/s41467-026-68660-5</a>","chicago":"Rak, Dmytro, Dusan Lorenc, Daniel Balazs, Ayan A. Zhumekenov, Osman M. Bakr, and Zhanybek Alpichshev. “Flexoelectric Domain Walls Enable Charge Separation and Transport in Cubic Perovskites.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-68660-5\">https://doi.org/10.1038/s41467-026-68660-5</a>.","apa":"Rak, D., Lorenc, D., Balazs, D., Zhumekenov, A. A., Bakr, O. M., &#38; Alpichshev, Z. (2026). Flexoelectric domain walls enable charge separation and transport in cubic perovskites. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-68660-5\">https://doi.org/10.1038/s41467-026-68660-5</a>","short":"D. Rak, D. Lorenc, D. Balazs, A.A. Zhumekenov, O.M. Bakr, Z. Alpichshev, Nature Communications 17 (2026).","ista":"Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. 2026. Flexoelectric domain walls enable charge separation and transport in cubic perovskites. Nature Communications. 17, 946.","mla":"Rak, Dmytro, et al. “Flexoelectric Domain Walls Enable Charge Separation and Transport in Cubic Perovskites.” <i>Nature Communications</i>, vol. 17, 946, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-68660-5\">10.1038/s41467-026-68660-5</a>."},"article_number":"946","oa_version":"Published Version","publisher":"Springer Nature","language":[{"iso":"eng"}],"ddc":["530"],"author":[{"id":"70313b46-47c2-11ec-9e88-cd79101918fe","full_name":"Rak, Dmytro","last_name":"Rak","first_name":"Dmytro"},{"full_name":"Lorenc, Dusan","id":"40D8A3E6-F248-11E8-B48F-1D18A9856A87","first_name":"Dusan","last_name":"Lorenc"},{"id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","full_name":"Balazs, Daniel","last_name":"Balazs","first_name":"Daniel","orcid":"0000-0001-7597-043X"},{"first_name":"Ayan A.","last_name":"Zhumekenov","full_name":"Zhumekenov, Ayan A."},{"full_name":"Bakr, Osman M.","last_name":"Bakr","first_name":"Osman M."},{"last_name":"Alpichshev","first_name":"Zhanybek","orcid":"0000-0002-7183-5203","id":"45E67A2A-F248-11E8-B48F-1D18A9856A87","full_name":"Alpichshev, Zhanybek"}],"file_date_updated":"2026-03-02T14:27:56Z","intvolume":"        17","publication_status":"published","oa":1,"file":[{"date_created":"2026-03-02T14:27:56Z","file_id":"21390","success":1,"checksum":"dd7a98de892d0b5abefca7e290ca0f77","creator":"dernst","access_level":"open_access","file_name":"2026_NatureComm_Rak.pdf","file_size":2570918,"relation":"main_file","date_updated":"2026-03-02T14:27:56Z","content_type":"application/pdf"}],"doi":"10.1038/s41467-026-68660-5","DOAJ_listed":"1","year":"2026","article_type":"original","date_created":"2026-03-02T10:06:58Z","day":"16","publication":"Nature Communications","OA_place":"publisher","corr_author":"1","pmid":1,"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","_id":"21382","department":[{"_id":"ZhAl"},{"_id":"LifeSc"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1"},{"volume":651,"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"PlanS_conform":"1","title":"Adventitious carbon breaks symmetry in oxide contact electrification","article_processing_charge":"Yes (via OA deal)","date_published":"2026-03-18T00:00:00Z","acknowledgement":"This project has received support from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 949120) and from the Marie Skłodowska-Curie programme (grant agreement no. 754411). We acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. N.M. acknowledges support from grant Fondecyt 1221597. G.G. is a Serra Húnter fellow. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility and Lab Support Facility. We thank the Modic group for the use of the Laue camera, T. Zauner for the photography of the experimental set-up and R. Möller for insightful discussions. Open access funding provided by Institute of Science and Technology (IST Austria).","external_id":{"pmid":["41851325"]},"quality_controlled":"1","date_updated":"2026-04-28T12:06:01Z","month":"03","citation":{"ista":"Grosjean GM, Ostermann M, Sauer M, Hahn M, Pichler CM, Fahrnberger F, Pertl F, Balazs D, Link MM, Kim SH, Schrader DL, Blanco A, Gracia F, Mujica N, Waitukaitis SR. 2026. Adventitious carbon breaks symmetry in oxide contact electrification. Nature. 651(8106), 626–631.","short":"G.M. Grosjean, M. Ostermann, M. Sauer, M. Hahn, C.M. Pichler, F. Fahrnberger, F. Pertl, D. Balazs, M.M. Link, S.H. Kim, D.L. Schrader, A. Blanco, F. Gracia, N. Mujica, S.R. Waitukaitis, Nature 651 (2026) 626–631.","mla":"Grosjean, Galien M., et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” <i>Nature</i>, vol. 651, no. 8106, Springer Nature, 2026, pp. 626–31, doi:<a href=\"https://doi.org/10.1038/s41586-025-10088-w\">10.1038/s41586-025-10088-w</a>.","chicago":"Grosjean, Galien M, Markus Ostermann, Markus Sauer, Michael Hahn, Christian M. Pichler, Florian Fahrnberger, Felix Pertl, et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” <i>Nature</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41586-025-10088-w\">https://doi.org/10.1038/s41586-025-10088-w</a>.","apa":"Grosjean, G. M., Ostermann, M., Sauer, M., Hahn, M., Pichler, C. M., Fahrnberger, F., … Waitukaitis, S. R. (2026). Adventitious carbon breaks symmetry in oxide contact electrification. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-10088-w\">https://doi.org/10.1038/s41586-025-10088-w</a>","ama":"Grosjean GM, Ostermann M, Sauer M, et al. Adventitious carbon breaks symmetry in oxide contact electrification. <i>Nature</i>. 2026;651(8106):626-631. doi:<a href=\"https://doi.org/10.1038/s41586-025-10088-w\">10.1038/s41586-025-10088-w</a>","ieee":"G. M. Grosjean <i>et al.</i>, “Adventitious carbon breaks symmetry in oxide contact electrification,” <i>Nature</i>, vol. 651, no. 8106. Springer Nature, pp. 626–631, 2026."},"abstract":[{"text":"Insulating oxides are among the most abundant solid materials in the universe1,2,3. Of the many ways in which they influence natural phenomena, perhaps the most consequential is their capacity to transfer electrical charge during contact4,5,6,7,8,9,10—which occurs even between samples of the same oxide—yet the symmetry-breaking parameter that causes this remains unidentified11,12. Here we show that adventitious carbonaceous molecules adsorbed from the environment are the symmetry-breaking factor in same-material oxide contact electrification (CE). We use acoustic levitation to measure charge exchange between a sphere and a plate composed of identical amorphous silicon dioxide (SiO2). Although charging polarity is random for co-prepared samples, we control it with baking or plasma treatment. Observing the charge-exchange relaxation afterwards, we see dynamics over a timescale of hours and connect this directly to the presence of adventitious carbon with time-of-flight mass spectrometry, low-energy ion scattering and infrared spectroscopy. Going further, we confirm that adventitious carbon can even determine charge exchange among different oxides. Our results identify the symmetry-breaking parameter that causes insulating oxides to exchange charge in settings ranging from desert sands4 to volcanic plumes5,6, while simultaneously highlighting an overlooked factor in CE more broadly.","lang":"eng"}],"oa_version":"Published Version","publisher":"Springer Nature","related_material":{"link":[{"description":"News on ISTA website","url":"https://ista.ac.at/en/news/colliding-dust-and-the-sparks-of-creation/","relation":"press_release"}]},"OA_type":"hybrid","type":"journal_article","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"status":"public","year":"2026","date_created":"2026-03-23T15:04:00Z","article_type":"original","file_date_updated":"2026-03-24T06:57:08Z","language":[{"iso":"eng"}],"ddc":["540"],"author":[{"full_name":"Grosjean, Galien M","id":"0C5FDA4A-9CF6-11E9-8939-FF05E6697425","orcid":"0000-0001-5154-417X","last_name":"Grosjean","first_name":"Galien M"},{"full_name":"Ostermann, Markus","first_name":"Markus","last_name":"Ostermann"},{"full_name":"Sauer, Markus","first_name":"Markus","last_name":"Sauer"},{"full_name":"Hahn, Michael","last_name":"Hahn","first_name":"Michael"},{"last_name":"Pichler","first_name":"Christian M.","full_name":"Pichler, Christian M."},{"last_name":"Fahrnberger","first_name":"Florian","full_name":"Fahrnberger, Florian"},{"first_name":"Felix","last_name":"Pertl","orcid":"0000-0003-0463-5794","id":"6313aec0-15b2-11ec-abd3-ed67d16139af","full_name":"Pertl, Felix"},{"id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","full_name":"Balazs, Daniel","first_name":"Daniel","last_name":"Balazs","orcid":"0000-0001-7597-043X"},{"full_name":"Link, Mason M.","last_name":"Link","first_name":"Mason M."},{"last_name":"Kim","first_name":"Seong H.","full_name":"Kim, Seong H."},{"full_name":"Schrader, Devin L.","last_name":"Schrader","first_name":"Devin L."},{"first_name":"Adriana","last_name":"Blanco","full_name":"Blanco, Adriana"},{"full_name":"Gracia, Francisco","first_name":"Francisco","last_name":"Gracia"},{"full_name":"Mujica, Nicolás","last_name":"Mujica","first_name":"Nicolás"},{"id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","full_name":"Waitukaitis, Scott R","first_name":"Scott R","last_name":"Waitukaitis","orcid":"0000-0002-2299-3176"}],"oa":1,"publication_status":"published","doi":"10.1038/s41586-025-10088-w","file":[{"date_created":"2026-03-24T06:57:08Z","file_id":"21494","success":1,"checksum":"dafef9ed575b44be4263e948a47ae056","creator":"dernst","access_level":"open_access","file_name":"2026_Nature_Grosjean.pdf","file_size":12245694,"relation":"main_file","content_type":"application/pdf","date_updated":"2026-03-24T06:57:08Z"}],"project":[{"_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","grant_number":"949120","call_identifier":"H2020","name":"Tribocharge: a multi-scale approach to an enduring problem in physics"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020"}],"intvolume":"       651","_id":"21485","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","ec_funded":1,"department":[{"_id":"ScWa"},{"_id":"GradSch"},{"_id":"LifeSc"}],"issue":"8106","pmid":1,"page":"626-631","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","day":"18","corr_author":"1","OA_place":"publisher","publication":"Nature"},{"abstract":[{"lang":"eng","text":"Background: Low-volume trapping columns are essential for sample enrichment, desalting, and injection profile focusing on nano-LC–MS-based proteomics. They enable higher sample loading, improve chromatographic performance, and protect the analytical column by removing salts and contaminants. Recently, monolithic trap columns with micropillar architecture have emerged as alternatives to conventionally packed traps. This study compares the performance of a packed and a micropillar monolithic trap column for the analysis of tryptic peptides. Methods: A tryptic digest of HeLa cell lysate was analyzed under identical LC–MS conditions using both trap types. Peptides were detected at 214 nm and analyzed by nano-ESI on a Q Exactive Plus Orbitrap. Data were searched against the human UniProt database (February 2023) using FragPipe v20.0, and statistical evaluation of MaxLFQ intensities was performed in Perseus using Welch’s t-test and clustering analysis. Results: Over 2500 proteins were identified with both setups. The packed trap column yielded more total peptides, particularly those with post-translational modifications and higher hydrophilicity, whereas the monolithic column favored peptides of intermediate hydrophobicity. Chromatographic profiles confirmed a slight reduction in the trapping efficiency of hydrophilic peptides by the monolithic trap. Conclusions: Trap column design significantly influences peptide recovery and proteome coverage."}],"article_number":"10","citation":{"ieee":"J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, and G. Mitulović, “Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses,” <i>Proteomes</i>, vol. 14, no. 1. MDPI, 2026.","ama":"Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses. <i>Proteomes</i>. 2026;14(1). doi:<a href=\"https://doi.org/10.3390/proteomes14010010\">10.3390/proteomes14010010</a>","chicago":"Miletić Vukajlović, Jadranka, Bojana Ilić, Bella Bruszel, Tanja Panić-Janković, and Goran Mitulović. “Comparison of the Trapping Efficiency for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” <i>Proteomes</i>. MDPI, 2026. <a href=\"https://doi.org/10.3390/proteomes14010010\">https://doi.org/10.3390/proteomes14010010</a>.","apa":"Miletić Vukajlović, J., Ilić, B., Bruszel, B., Panić-Janković, T., &#38; Mitulović, G. (2026). Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses. <i>Proteomes</i>. MDPI. <a href=\"https://doi.org/10.3390/proteomes14010010\">https://doi.org/10.3390/proteomes14010010</a>","short":"J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, G. Mitulović, Proteomes 14 (2026).","ista":"Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. 2026. Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses. Proteomes. 14(1), 10.","mla":"Miletić Vukajlović, Jadranka, et al. “Comparison of the Trapping Efficiency for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” <i>Proteomes</i>, vol. 14, no. 1, 10, MDPI, 2026, doi:<a href=\"https://doi.org/10.3390/proteomes14010010\">10.3390/proteomes14010010</a>."},"scopus_import":"1","oa_version":"Published Version","publisher":"MDPI","OA_type":"gold","type":"journal_article","publication_identifier":{"eissn":["2227-7382"]},"status":"public","volume":14,"title":"Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses","PlanS_conform":"1","date_published":"2026-03-01T00:00:00Z","article_processing_charge":"Yes","acknowledgement":"The authors thank Gábor Tóth, Uppsala University, Sweden, and Armel Nicolas, Institute for Science and Technology Austria, for their support. This research was conducted during a student residency in Vienna under the auspices of OeAD. ZI: ICM-2016-03196.","external_id":{"pmid":["41893725"]},"quality_controlled":"1","date_updated":"2026-05-04T10:36:21Z","month":"03","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21711","department":[{"_id":"MassSpec"}],"issue":"1","pmid":1,"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","day":"01","OA_place":"publisher","publication":"Proteomes","year":"2026","DOAJ_listed":"1","date_created":"2026-04-12T22:01:49Z","article_type":"original","file_date_updated":"2026-05-04T10:31:35Z","language":[{"iso":"eng"}],"ddc":["540"],"author":[{"full_name":"Miletić Vukajlović, Jadranka","last_name":"Miletić Vukajlović","first_name":"Jadranka"},{"last_name":"Ilić","first_name":"Bojana","full_name":"Ilić, Bojana"},{"last_name":"Bruszel","first_name":"Bella","id":"70abbbb3-88ea-11ec-8e0a-e8c939944834","full_name":"Bruszel, Bella"},{"last_name":"Panić-Janković","first_name":"Tanja","full_name":"Panić-Janković, Tanja"},{"first_name":"Goran","last_name":"Mitulović","full_name":"Mitulović, Goran"}],"oa":1,"publication_status":"published","file":[{"success":1,"file_id":"21790","date_created":"2026-05-04T10:31:35Z","file_name":"2026_Proteomes_Vukajlovic.pdf","access_level":"open_access","checksum":"1e0c66bbf4b6e0be626a8639ea664b63","creator":"dernst","date_updated":"2026-05-04T10:31:35Z","content_type":"application/pdf","relation":"main_file","file_size":1009723}],"doi":"10.3390/proteomes14010010","intvolume":"        14"},{"quality_controlled":"1","date_updated":"2026-05-04T12:27:06Z","external_id":{"pmid":["41964955 "]},"month":"04","volume":45,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"date_published":"2026-04-28T00:00:00Z","article_processing_charge":"Yes","acknowledgement":"We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).","title":"Multifold increase in spinal inhibitory cell types with emergence of limb movement","PlanS_conform":"1","OA_type":"gold","status":"public","type":"journal_article","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"abstract":[{"lang":"eng","text":"As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution."}],"scopus_import":"1","article_number":"117227","citation":{"apa":"Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>","chicago":"Vijatovic, David, Florina Alexandra  Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>.","mla":"Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>, vol. 45, no. 4, 117227, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>.","ista":"Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227.","short":"D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).","ieee":"D. Vijatovic <i>et al.</i>, “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” <i>Cell Reports</i>, vol. 45, no. 4. Elsevier, 2026.","ama":"Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. 2026;45(4). doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>"},"oa_version":"Published Version","publisher":"Elsevier","ddc":["570"],"language":[{"iso":"eng"}],"author":[{"full_name":"Vijatovic, David","id":"cf391e77-ec3c-11ea-a124-d69323410b58","first_name":"David","last_name":"Vijatovic"},{"last_name":"Toma","first_name":"Florina Alexandra ","full_name":"Toma, Florina Alexandra ","id":"2f73f876-f128-11eb-9611-b96b5a30cb0e"},{"first_name":"Y","last_name":"Ignatyev","full_name":"Ignatyev, Y"},{"id":"a8144562-32c9-11ee-b5ce-d9800628bda2","full_name":"Harrington, Zoe P","first_name":"Zoe P","last_name":"Harrington","orcid":"0009-0008-0158-4032"},{"orcid":"0000-0003-1216-9105","first_name":"Christoph M","last_name":"Sommer","full_name":"Sommer, Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert","orcid":"0000-0001-9843-3522"},{"id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","full_name":"Smits, Matthijs Geert","first_name":"Matthijs Geert","last_name":"Smits"},{"full_name":"Dalla Vecchia, Marco","id":"02a7a869-ff06-11ed-a87f-86649d6077e5","first_name":"Marco","last_name":"Dalla Vecchia"},{"full_name":"Trevisan, Alexandra J.","last_name":"Trevisan","first_name":"Alexandra J."},{"full_name":"Chapman, Phillip","last_name":"Chapman","first_name":"Phillip"},{"first_name":"Mara","last_name":"Julseth","full_name":"Julseth, Mara","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1"},{"full_name":"Brenner-Morton, Susan","last_name":"Brenner-Morton","first_name":"Susan"},{"first_name":"Mariano I.","last_name":"Gabitto","full_name":"Gabitto, Mariano I."},{"last_name":"Dasen","first_name":"Jeremy S.","full_name":"Dasen, Jeremy S."},{"full_name":"Bikoff, Jay B.","first_name":"Jay B.","last_name":"Bikoff"},{"orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"}],"file_date_updated":"2026-05-04T12:20:10Z","intvolume":"        45","oa":1,"publication_status":"published","file":[{"file_size":14925958,"relation":"main_file","date_updated":"2026-05-04T12:20:10Z","content_type":"application/pdf","date_created":"2026-05-04T12:20:10Z","file_id":"21795","success":1,"checksum":"0d26cdb5b8d8dec3a911d8261a65cdef","creator":"dernst","access_level":"open_access","file_name":"2026_CellReports_Vijatovic.pdf"}],"project":[{"grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity"},{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"},{"_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"}],"doi":"10.1016/j.celrep.2026.117227","DOAJ_listed":"1","year":"2026","article_type":"original","date_created":"2026-04-19T22:07:43Z","day":"28","publication":"Cell Reports","OA_place":"publisher","corr_author":"1","pmid":1,"_id":"21746","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"4","department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1"},{"volume":392,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"title":"Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape","date_published":"2026-04-16T00:00:00Z","article_processing_charge":"No","acknowledgement":"We thank all members of the Loose lab at ISTA for helpful discussions; M. Kojic for critical reading of the manuscript; A. Herrero (Sevilla University) for sharing her extensive BACTH plasmid library and other plasmids, as well as cyanobacterial strains; T. Dagan and F. Nies (both Kiel University) for sharing cyanobacterial strains and plasmids and for valuable discussions; N. Sapay and A. Michon for providing the Amphipaseek code, which enabled us to perform our large-scale amphipathic helix screen of cyanobacterial CorR proteins; V.-V. Hodirnau for support in cryo-ET data collection; and J. Hansen for advice about cryo-EM data processing.\r\nThis work was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF), the Scientific Computing (SciComp), the Electron Microscopy Facility (EMF), and the Lab Support Facility (LSF). This work was funded by the European Union’s Horizon 2020 research and innovation program (Marie Skłodowska-Curie grant 101034413 to B.L.S.); the European Research Council (ERC) of the European Union (grant ActinID 101076260 to F.K.M.S.); the Swiss National Science Foundation (starting grant TMSGI3_226208 to G.L.W.); and the Jean-Jacques et Letitia Lopez-Loreta Foundation (G.L.W.).","external_id":{"pmid":["41990175"]},"date_updated":"2026-04-28T13:29:05Z","quality_controlled":"1","month":"04","scopus_import":"1","citation":{"ieee":"B. L. Springstein <i>et al.</i>, “Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape,” <i>Science</i>, vol. 392, no. 6795. AAAS, 2026.","ama":"Springstein BL, Javoor M, Megrian D, et al. Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape. <i>Science</i>. 2026;392(6795). doi:<a href=\"https://doi.org/10.1126/science.aea6343\">10.1126/science.aea6343</a>","apa":"Springstein, B. L., Javoor, M., Megrian, D., Hajdu, R., Hanke, D. M., Zens, B., … Loose, M. (2026). Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.aea6343\">https://doi.org/10.1126/science.aea6343</a>","chicago":"Springstein, Benjamin L, Manjunath Javoor, Daniela Megrian, Roman Hajdu, Dustin M. Hanke, Bettina Zens, Gregor L. Weiss, Florian KM Schur, and Martin Loose. “Repurposing of a DNA Segregation Machinery into a Cytoskeletal System Controlling Cell Shape.” <i>Science</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/science.aea6343\">https://doi.org/10.1126/science.aea6343</a>.","mla":"Springstein, Benjamin L., et al. “Repurposing of a DNA Segregation Machinery into a Cytoskeletal System Controlling Cell Shape.” <i>Science</i>, vol. 392, no. 6795, eaea6343, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/science.aea6343\">10.1126/science.aea6343</a>.","short":"B.L. Springstein, M. Javoor, D. Megrian, R. Hajdu, D.M. Hanke, B. Zens, G.L. Weiss, F.K. Schur, M. Loose, Science 392 (2026).","ista":"Springstein BL, Javoor M, Megrian D, Hajdu R, Hanke DM, Zens B, Weiss GL, Schur FK, Loose M. 2026. Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape. Science. 392(6795), eaea6343."},"abstract":[{"text":"Bacteria, like eukaryotes, use conserved cytoskeletal systems for intracellular organization. The plasmid-encoded ParMRC system forms actin-like filaments that segregate low–copy number plasmids. In multicellular cyanobacteria such as Anabaena sp., we found that a chromosomally encoded ParMR system has evolved into a cytoskeletal system named CorMR with a function in cell shape control rather than DNA segregation. Live-cell imaging, in vitro reconstitution, and cryo–electron microscopy revealed that CorM formed dynamically unstable, antiparallel double-stranded filaments that were recruited to the membrane by CorR through an amphipathic helix conserved in multicellular cyanobacteria. CorMR filaments were regulated by MinC, which excluded them from the poles and division plane. Comparative genomics indicated that the repurposing of ParMR and Min systems coevolved with cyanobacterial multicellularity, highlighting the evolutionary plasticity of cytoskeletal systems in bacteria.","lang":"eng"}],"article_number":"eaea6343","oa_version":"None","publisher":"AAAS","OA_type":"closed access","type":"journal_article","publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"status":"public","year":"2026","date_created":"2026-04-26T22:01:46Z","article_type":"original","language":[{"iso":"eng"}],"author":[{"last_name":"Springstein","first_name":"Benjamin L","orcid":"0000-0002-3461-5391","id":"b4eb62ef-ac72-11ed-9503-ed3b4d66c083","full_name":"Springstein, Benjamin L"},{"id":"305ab18b-dc7d-11ea-9b2f-b58195228ea2","full_name":"Javoor, Manjunath","first_name":"Manjunath","last_name":"Javoor","orcid":"0000-0003-2311-2112"},{"last_name":"Megrian","first_name":"Daniela","full_name":"Megrian, Daniela"},{"first_name":"Roman","last_name":"Hajdu","id":"ffab949d-133f-11ed-8f02-94de21ace503","full_name":"Hajdu, Roman"},{"last_name":"Hanke","first_name":"Dustin M.","full_name":"Hanke, Dustin M."},{"first_name":"Bettina","last_name":"Zens","orcid":"0000-0002-9561-1239","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","full_name":"Zens, Bettina"},{"full_name":"Weiss, Gregor L.","last_name":"Weiss","first_name":"Gregor L."},{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","full_name":"Schur, Florian Km","last_name":"Schur","first_name":"Florian Km","orcid":"0000-0003-4790-8078"},{"first_name":"Martin","last_name":"Loose","orcid":"0000-0001-7309-9724","id":"462D4284-F248-11E8-B48F-1D18A9856A87","full_name":"Loose, Martin"}],"publication_status":"published","doi":"10.1126/science.aea6343","project":[{"name":"IST-BRIDGE: International postdoctoral program","call_identifier":"H2020","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413"},{"_id":"bd980d18-d553-11ed-ba76-ceaa645c97eb","grant_number":"101076260","name":"A molecular atlas of Actin filament IDentities in the cell motility machinery"}],"intvolume":"       392","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21762","ec_funded":1,"issue":"6795","department":[{"_id":"MaLo"},{"_id":"FlSc"},{"_id":"GradSch"},{"_id":"EM-Fac"}],"pmid":1,"day":"16","corr_author":"1","publication":"Science"},{"year":"2026","article_type":"original","date_created":"2026-04-26T22:01:47Z","author":[{"first_name":"Fernando","last_name":"Gonzalez-Uarquin","full_name":"Gonzalez-Uarquin, Fernando"},{"full_name":"Jirkof, Paulin","first_name":"Paulin","last_name":"Jirkof"},{"full_name":"Bert, Bettina","last_name":"Bert","first_name":"Bettina"},{"full_name":"Hawkins, Penny","first_name":"Penny","last_name":"Hawkins"},{"full_name":"Angelovski, Ljupco","last_name":"Angelovski","first_name":"Ljupco"},{"full_name":"Baumgart, Jan","last_name":"Baumgart","first_name":"Jan"},{"last_name":"Baumgart","first_name":"Nadine","full_name":"Baumgart, Nadine"},{"full_name":"Cevik, Özge S.","first_name":"Özge S.","last_name":"Cevik"},{"full_name":"Franco, Nuno H.","first_name":"Nuno H.","last_name":"Franco"},{"first_name":"Erdal","last_name":"Horata","full_name":"Horata, Erdal"},{"first_name":"Rohish","last_name":"Kaura","full_name":"Kaura, Rohish"},{"last_name":"Neuhaus","first_name":"Winfried","full_name":"Neuhaus, Winfried"},{"full_name":"Riso, Brigida","last_name":"Riso","first_name":"Brigida"},{"first_name":"Adrian J.","last_name":"Smith","full_name":"Smith, Adrian J."},{"last_name":"Sotiropoulos","first_name":"Athanassia","full_name":"Sotiropoulos, Athanassia"},{"last_name":"Vitale","first_name":"Augusto","full_name":"Vitale, Augusto"},{"full_name":"Schober, Sophie","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","last_name":"Schober","first_name":"Sophie"}],"language":[{"iso":"eng"}],"ddc":["570"],"doi":"10.1177/00236772251400976","publication_status":"epub_ahead","oa":1,"department":[{"_id":"PreCl"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21767","day":"14","publication":"Laboratory Animals","OA_place":"publisher","acknowledgement":"We deeply acknowledge all the animal care staff and laboratory technicians who participated in this study! We acknowledge Working Groups 1 and 4 from COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”), CA21139, supported by COST (European Cooperation in Science and Technology) for their feedback and support. We also acknowledge Aoife Milford for her comments and contributions to the final draft of the manuscript.\r\nThis publication was based on work from the COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”), CA21139, supported by COST (European Cooperation in Science and Technology).","date_published":"2026-04-14T00:00:00Z","article_processing_charge":"Yes (in subscription journal)","title":"Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science","quality_controlled":"1","date_updated":"2026-06-18T08:33:36Z","month":"04","oa_version":"Published Version","abstract":[{"lang":"eng","text":"The involvement of non-scientific staff in discussions about animal welfare and scientific quality is essential for biomedical research progress. In this study, we developed a survey to collect the self-perception of animal care staff (ACS) and laboratory technicians about their involvement in scientific planning and conduct. Participants were contacted to complete an anonymous online questionnaire. We obtained 850 responses, mainly from Europe: 564 from ACS and 286 from laboratory technicians. Job satisfaction was assessed as positive by ACS and laboratory technicians despite the low frequency of culture of care activities and mental health meetings. Both groups expressed their desire to be trained in research planning and conduct; however, regular training was not reported. In addition, the inability to act on animal welfare concerns owing to experimental reasons was reported by both groups. Over half of the participants felt valued and appreciated by the lead scientists or animal facility manager; however, it is not clear how they are acknowledged, as their names on the authors list or in the manuscript acknowledgments are barely included. Our results indicated that involvement of ACS and laboratory technicians in planning and conducting studies would improve their understanding of how experiments are done, and therefore communication processes, work satisfaction, animal welfare, and scientific quality. Finally, we provided recommendations to improve the engagement of ACS and laboratory technicians in discussions about animal research planning and conduct."},{"lang":"fre","text":"La participation de personnel non-scientifique aux discussions sur le bien-être animal et la qualité scientifique est essentielle aux progrès la recherche biomédicale. Dans cette étude, nous avons développé une enquête pour recueillir l'auto-perception du personnel chargé des soins prodigués aux animaux (PCSA) et des techniciens de laboratoire (TL) sur leur implication dans la planification et la conduite scientifiques. Les participants ont été contactés pour remplir un questionnaire anonyme en ligne. Nous avons obtenu 850 réponses, principalement en Europe : 564 provenant de PCSA et 286 de TL. La satisfaction au travail a été évaluée comme positive par le PCSA et les TL malgré la faible fréquence d’activités sur la culture des soins et de réunions concernant la santé mentale. Bien que les deux groupes aient exprimé leur désir d'être formés à la planification et à la conduite de la recherche, aucune formation réelle régulière n'a été signalée. De plus, l'incapacité d'agir sur les préoccupations relatives au bien-être animal pour des raisons expérimentales a été signalée par les deux groupes. Plus de la moitié des participants se sont sentis valorisés et appréciés par les scientifiques principaux ou le gestionnaire de l’installation animale mais on ne sait pas clairement comment ils sont reconnus, car leurs noms sur la liste des auteurs ou dans les remerciements sont à peine inclus dans la documentation. Nos résultats ont indiqué que la participation du PCSA et des TL à la planification et à la conduite des études améliorerait leur compréhension de la façon dont les expériences sont effectuées et, par conséquent, les processus de communication, leur satisfaction au travail ainsi que le bien-être animal et la qualité scientifique. Enfin, nous avons formulé des recommandations pour améliorer la participation du PCSA et des TL aux discussions sur la planification et la conduite de la recherche animale."},{"text":"Die Einbeziehung von nichtwissenschaftlichem Personal in Diskussionen über Tierschutz und wissenschaftliche Qualität ist für Fortschritte in biomedizinischer Forschung von entscheidender Bedeutung. In dieser Studie haben wir eine Umfrage entwickelt, um die Selbsteinschätzung von Tierpflegern (ACS) und Labortechnikern (LT) hinsichtlich ihrer Beteiligung an der wissenschaftlichen Planung und Durchführung zu erfassen. Die Teilnehmer wurden gebeten, einen anonymen Online-Fragebogen auszufüllen. Wir erhielten 850 Rückmeldungen, hauptsächlich aus Europa: 564 von ACS und 286 von LT. Die Arbeitszufriedenheit wurde von ACS und LT trotz der geringen Häufigkeit von Pflegeaktivitäten und Treffen zum Thema psychische Gesundheit als positiv bewertet. Beide Gruppen äußerten den Wunsch, in der Forschungsplanung und -durchführung geschult zu werden, doch regelmäßig stattfindende Schulungen wurden nicht berichtet. Außerdem wurde von beiden Gruppen vermeldet, dass sie aus versuchstechnischen Gründen nicht in der Lage waren, auf Tierschutzbedenken zu reagieren. Über die Hälfte der Teilnehmer fühlte sich von den leitenden Wissenschaftlern oder dem Leiter der Tierhaltungseinrichtung geschätzt und anerkannt; es ist jedoch unklar, inwiefern sie wirklich gewürdigt werden, da ihre Namen kaum in der Autorenliste oder in den Danksagungen des Manuskripts aufgeführt sind. Unsere Ergebnisse deuteten darauf hin, dass die Einbeziehung von ACS und LT in die Planung und Durchführung von Studien ihr Verständnis für die Durchführung von Experimenten verbessern würde – und damit auch Kommunikationsprozesse, Arbeitszufriedenheit, Tierwohl und wissenschaftliche Qualität. Abschließend gaben wir Empfehlungen zur Verbesserung der Einbeziehung von ACS und LT in Diskussionen über die Planung und Durchführung von Tierversuchen.","lang":"ger"},{"lang":"spa","text":"La participación del personal no científico en los debates sobre el bienestar animal y la calidad científica es fundamental para el avance de la investigación biomédica. En este estudio, desarrollamos una encuesta para recoger la autopercepción del personal encargado del cuidado de los animales (ACS) y de los técnicos de laboratorio (LT) sobre su implicación en la planificación y la realización científicas. Se contactó con los participantes para que cumplimentaran un cuestionario anónimo en línea. Obtuvimos 850 respuestas, principalmente de Europa: 564 de ACS y 286 de LT. La satisfacción laboral fue evaluada como positiva por ACS y técnicos de laboratorio a pesar de la baja frecuencia de actividades de cultura del cuidado y reuniones sobre bienestar mental. Ambos grupos expresaron su deseo de recibir formación en planificación y realización de investigaciones, sin embargo, no se informó sobre una formación regular. Asimismo, ambos grupos señalaron la incapacidad de actuar ante las preocupaciones sobre el bienestar animal por motivos experimentales. Más de la mitad de los participantes se sintieron valorados y apreciados por los científicos principales o el responsable de las instalaciones de animales; sin embargo, no está claro cómo se les reconoce, ya que apenas se incluyen sus nombres en la lista de autores o en los agradecimientos del manuscrito. Nuestros resultados indicaron que la participación de los ACS y los LT en la planificación y realización de los estudios mejoraría su comprensión de cómo se hacen los experimentos y, por tanto, los procesos de comunicación, la satisfacción laboral, el bienestar animal y la calidad científica. Finalmente, proporcionamos recomendaciones para mejorar el compromiso de la AEC y la LT en los debates sobre la planificación y la realización de investigaciones con animales."}],"scopus_import":"1","citation":{"ieee":"F. Gonzalez-Uarquin <i>et al.</i>, “Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science,” <i>Laboratory Animals</i>. SAGE Publications, 2026.","ama":"Gonzalez-Uarquin F, Jirkof P, Bert B, et al. Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science. <i>Laboratory Animals</i>. 2026. doi:<a href=\"https://doi.org/10.1177/00236772251400976\">10.1177/00236772251400976</a>","chicago":"Gonzalez-Uarquin, Fernando, Paulin Jirkof, Bettina Bert, Penny Hawkins, Ljupco Angelovski, Jan Baumgart, Nadine Baumgart, et al. “Building Bridges: Involvement of Animal Care Staff and Laboratory Technicians in Experimental Planning and Conduct of Animal Studies for Better Job Satisfaction and Science.” <i>Laboratory Animals</i>. SAGE Publications, 2026. <a href=\"https://doi.org/10.1177/00236772251400976\">https://doi.org/10.1177/00236772251400976</a>.","apa":"Gonzalez-Uarquin, F., Jirkof, P., Bert, B., Hawkins, P., Angelovski, L., Baumgart, J., … Schober, S. (2026). Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science. <i>Laboratory Animals</i>. SAGE Publications. <a href=\"https://doi.org/10.1177/00236772251400976\">https://doi.org/10.1177/00236772251400976</a>","short":"F. Gonzalez-Uarquin, P. Jirkof, B. Bert, P. Hawkins, L. Angelovski, J. Baumgart, N. Baumgart, Ö.S. Cevik, N.H. Franco, E. Horata, R. Kaura, W. Neuhaus, B. Riso, A.J. Smith, A. Sotiropoulos, A. Vitale, S. Schober, Laboratory Animals (2026).","ista":"Gonzalez-Uarquin F, Jirkof P, Bert B, Hawkins P, Angelovski L, Baumgart J, Baumgart N, Cevik ÖS, Franco NH, Horata E, Kaura R, Neuhaus W, Riso B, Smith AJ, Sotiropoulos A, Vitale A, Schober S. 2026. Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science. Laboratory Animals.","mla":"Gonzalez-Uarquin, Fernando, et al. “Building Bridges: Involvement of Animal Care Staff and Laboratory Technicians in Experimental Planning and Conduct of Animal Studies for Better Job Satisfaction and Science.” <i>Laboratory Animals</i>, SAGE Publications, 2026, doi:<a href=\"https://doi.org/10.1177/00236772251400976\">10.1177/00236772251400976</a>."},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1177/00236772251400976"}],"publisher":"SAGE Publications","OA_type":"hybrid","status":"public","publication_identifier":{"issn":["0023-6772"],"eissn":["1758-1117"]},"type":"journal_article"},{"year":"2026","DOAJ_listed":"1","date_created":"2026-05-03T22:01:37Z","article_type":"original","file_date_updated":"2026-05-07T08:21:06Z","ddc":["570"],"language":[{"iso":"eng"}],"author":[{"full_name":"Vignolle, Anna","last_name":"Vignolle","first_name":"Anna"},{"full_name":"Zehl, Martin","id":"8e016d5b-5d77-11f0-86d2-96cdb3922a55","orcid":"0000-0001-9685-0373","first_name":"Martin","last_name":"Zehl"},{"full_name":"Garzón, Jaime Felipe Guerrero","last_name":"Garzón","first_name":"Jaime Felipe Guerrero"},{"first_name":"Olha","last_name":"Schneider","full_name":"Schneider, Olha"},{"full_name":"Gafriller, Johannes","last_name":"Gafriller","first_name":"Johannes"},{"last_name":"Grienke","first_name":"Ulrike","full_name":"Grienke, Ulrike"},{"last_name":"Kirkegaard","first_name":"Rasmus H.","full_name":"Kirkegaard, Rasmus H."},{"full_name":"Zotchev, Sergey B.","first_name":"Sergey B.","last_name":"Zotchev"}],"publication_status":"published","oa":1,"doi":"10.1111/1751-7915.70357","file":[{"file_size":575492,"date_updated":"2026-05-07T08:21:06Z","relation":"main_file","content_type":"application/pdf","checksum":"8c8aa660cef5394167e06f187adbabf0","creator":"dernst","file_name":"2026_MicrobialBiotechnology_Vignolle.pdf","access_level":"open_access","date_created":"2026-05-07T08:21:06Z","success":1,"file_id":"21835"}],"intvolume":"        19","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21779","issue":"4","department":[{"_id":"MassSpec"}],"pmid":1,"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","day":"01","OA_place":"publisher","publication":"Microbial Biotechnology","volume":19,"title":"Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin","article_processing_charge":"Yes","date_published":"2026-04-01T00:00:00Z","acknowledgement":"This work was supported by the University of Vienna. The authors thank Anna Fabisikova from the Mass Spectrometry Centre and the team of the NMR Centre (both of the Faculty of Chemistry, University of Vienna and members of the Vienna Life Science Instruments) for assistance with data acquisition. Open Access funding provided by Universitat Wien. This work was supported by Universität Wien.","external_id":{"pmid":["42036976"]},"date_updated":"2026-05-07T08:22:41Z","quality_controlled":"1","month":"04","citation":{"apa":"Vignolle, A., Zehl, M., Garzón, J. F. G., Schneider, O., Gafriller, J., Grienke, U., … Zotchev, S. B. (2026). Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. <i>Microbial Biotechnology</i>. Wiley. <a href=\"https://doi.org/10.1111/1751-7915.70357\">https://doi.org/10.1111/1751-7915.70357</a>","chicago":"Vignolle, Anna, Martin Zehl, Jaime Felipe Guerrero Garzón, Olha Schneider, Johannes Gafriller, Ulrike Grienke, Rasmus H. Kirkegaard, and Sergey B. Zotchev. “Identification and Characterisation of the Gene Cluster Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” <i>Microbial Biotechnology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/1751-7915.70357\">https://doi.org/10.1111/1751-7915.70357</a>.","mla":"Vignolle, Anna, et al. “Identification and Characterisation of the Gene Cluster Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” <i>Microbial Biotechnology</i>, vol. 19, no. 4, e70357, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/1751-7915.70357\">10.1111/1751-7915.70357</a>.","short":"A. Vignolle, M. Zehl, J.F.G. Garzón, O. Schneider, J. Gafriller, U. Grienke, R.H. Kirkegaard, S.B. Zotchev, Microbial Biotechnology 19 (2026).","ista":"Vignolle A, Zehl M, Garzón JFG, Schneider O, Gafriller J, Grienke U, Kirkegaard RH, Zotchev SB. 2026. Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial Biotechnology. 19(4), e70357.","ieee":"A. Vignolle <i>et al.</i>, “Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin,” <i>Microbial Biotechnology</i>, vol. 19, no. 4. Wiley, 2026.","ama":"Vignolle A, Zehl M, Garzón JFG, et al. Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. <i>Microbial Biotechnology</i>. 2026;19(4). doi:<a href=\"https://doi.org/10.1111/1751-7915.70357\">10.1111/1751-7915.70357</a>"},"scopus_import":"1","abstract":[{"text":"Acidomycin is an anti-mycobacterial antibiotic with a unique mode of action, targeting the biotin biosynthesis pathway. Despite being highly active against mycobacteria in vitro, its development as an anti-tubercular agent has been hindered due to suboptimal pharmacokinetics. Engineering of the acidomycin biosynthesis may yield new analogues with improved pharmacological properties. Here, we describe the identification of the acidomycin biosynthetic gene cluster (BGC) in a Streptomyces bacterium isolated from the rhizosphere of Edelweiss. Notably, the acidomycin BGC is located in proximity to the genes for the biosynthesis of stravidins, secondary metabolites targeting a different enzyme in the biotin biosynthesis pathway, and two genes for streptavidins, proteins that strongly bind and sequester biotin. The identity of the acidomycin BGC was confirmed via both gene knock-out and heterologous expression, which suggested that the fatty acid required for the formation of acidomycin's acyl chain is most likely scavenged from the biotin biosynthesis pathway. CRISPR/Cas9-assisted knock-out of the cytochrome P450-encoding gene in the acidomycin BGC resulted in a significant decrease in its yield but did not abrogate the biosynthesis completely.","lang":"eng"}],"article_number":"e70357","oa_version":"Published Version","publisher":"Wiley","OA_type":"gold","type":"journal_article","publication_identifier":{"eissn":["1751-7915"]},"status":"public"},{"_id":"21848","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"PreCl"}],"OA_place":"repository","publication":"Journal of Investigative Dermatology","day":"07","date_created":"2026-05-10T22:02:16Z","article_type":"original","year":"2026","publication_status":"inpress","oa":1,"doi":"10.1016/j.jid.2026.03.026","language":[{"iso":"eng"}],"author":[{"full_name":"Klein, Klara","first_name":"Klara","last_name":"Klein"},{"full_name":"Johnson, Litty","last_name":"Johnson","first_name":"Litty"},{"last_name":"Rîca","first_name":"Ramona","full_name":"Rîca, Ramona"},{"full_name":"Sarcevic, Mirza","last_name":"Sarcevic","first_name":"Mirza"},{"last_name":"Carta","first_name":"Gabriele","full_name":"Carta, Gabriele"},{"full_name":"Seiser, Saskia","first_name":"Saskia","last_name":"Seiser"},{"first_name":"Adelheid","last_name":"Elbe-Bürger","full_name":"Elbe-Bürger, Adelheid"},{"last_name":"Langer","first_name":"Freyja","id":"3C1BE782-F248-11E8-B48F-1D18A9856A87","full_name":"Langer, Freyja"},{"last_name":"Rahhal","first_name":"Nowras","full_name":"Rahhal, Nowras"},{"full_name":"Rademacher, Christoph","first_name":"Christoph","last_name":"Rademacher"},{"full_name":"Wawrzinek, Robert","last_name":"Wawrzinek","first_name":"Robert"},{"first_name":"Federica","last_name":"Quattrone","full_name":"Quattrone, Federica"},{"full_name":"Sparber, Florian","first_name":"Florian","last_name":"Sparber"}],"publisher":"Elsevier","main_file_link":[{"url":"https://doi.org/10.1101/2025.06.25.661517","open_access":"1"}],"scopus_import":"1","citation":{"ieee":"K. Klein <i>et al.</i>, “Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity,” <i>Journal of Investigative Dermatology</i>. Elsevier.","ama":"Klein K, Johnson L, Rîca R, et al. Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity. <i>Journal of Investigative Dermatology</i>. doi:<a href=\"https://doi.org/10.1016/j.jid.2026.03.026\">10.1016/j.jid.2026.03.026</a>","apa":"Klein, K., Johnson, L., Rîca, R., Sarcevic, M., Carta, G., Seiser, S., … Sparber, F. (n.d.). Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity. <i>Journal of Investigative Dermatology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jid.2026.03.026\">https://doi.org/10.1016/j.jid.2026.03.026</a>","chicago":"Klein, Klara, Litty Johnson, Ramona Rîca, Mirza Sarcevic, Gabriele Carta, Saskia Seiser, Adelheid Elbe-Bürger, et al. “Langerhans Cell–Targeted MRNA Delivery: A Strategy for Dose-Sparing and Enhanced Antitumor Immunity.” <i>Journal of Investigative Dermatology</i>. Elsevier, n.d. <a href=\"https://doi.org/10.1016/j.jid.2026.03.026\">https://doi.org/10.1016/j.jid.2026.03.026</a>.","mla":"Klein, Klara, et al. “Langerhans Cell–Targeted MRNA Delivery: A Strategy for Dose-Sparing and Enhanced Antitumor Immunity.” <i>Journal of Investigative Dermatology</i>, Elsevier, doi:<a href=\"https://doi.org/10.1016/j.jid.2026.03.026\">10.1016/j.jid.2026.03.026</a>.","ista":"Klein K, Johnson L, Rîca R, Sarcevic M, Carta G, Seiser S, Elbe-Bürger A, Langer F, Rahhal N, Rademacher C, Wawrzinek R, Quattrone F, Sparber F. Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity. Journal of Investigative Dermatology.","short":"K. Klein, L. Johnson, R. Rîca, M. Sarcevic, G. Carta, S. Seiser, A. Elbe-Bürger, F. Langer, N. Rahhal, C. Rademacher, R. Wawrzinek, F. Quattrone, F. Sparber, Journal of Investigative Dermatology (n.d.)."},"abstract":[{"lang":"eng","text":"Despite the success of mRNA therapeutics, challenges remain in optimizing immune responses and minimizing side effects. Cell-specific antigen delivery may help reduce required doses and improve vaccine efficacy. In this study, we report on a targeted delivery system for mRNA to a specific subset of skin-resident antigen-presenting cells: Langerhans cells. By functionalizing lipid nanoparticles with a langerin-specific glycomimetic ligand, we achieve selective mRNA delivery to both murine and human primary Langerhans cells with minimal off-target uptake, at the same time resulting in significantly increased mRNA translation. This targeted mRNA delivery not only enhances antigen presentation and T-cell responses but also enables dose-sparing and superior antitumor immunity compared with conventional immunization in a B16-OVA tumor model. Importantly, our platform’s high compatibility with various lipid nanoparticle formulations offers a flexible and precise tool for skin-directed mRNA delivery."}],"oa_version":"Preprint","type":"journal_article","publication_identifier":{"eissn":["1523-1747"],"issn":["0022-202X"]},"status":"public","OA_type":"green","title":"Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity","article_processing_charge":"No","date_published":"2026-04-07T00:00:00Z","acknowledgement":"We thank Mareike Rentzsch for her intellectual contributions during the course of our discussions. We thank Michael Schunn from the Preclinical Facility of the Institute of Science and Technology Austria for his continuous technical support. Guarantor of the work is FS. This project was supported by “Seedfinancing” (P2282679) of the Austrian Federal Ministry of Digital and Economic Affairs and the Ministry of Climate Action and Energy, handled by the Austrian Wirtschaftsservice, as well as by...","acknowledged_ssus":[{"_id":"PreCl"}],"month":"04","date_updated":"2026-05-11T06:07:32Z"},{"external_id":{"pmid":["41834724"]},"date_updated":"2026-05-12T06:40:18Z","quality_controlled":"1","month":"04","volume":139,"title":"α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures","PlanS_conform":"1","acknowledgement":"The technical assistance by Tanja Wagner and Elena Lilliu is gratefully acknowledged. This research was funded in whole or in part by the Austrian Science Fund (FWF) (P36145 to H.K., PAT8605623 to M.H. and P33799 to A.V.K.]. Open Access funding provided by Medical University of Vienna and the Austrian Science Fund (FWF). Deposited in PMC for immediate release.","date_published":"2026-04-27T00:00:00Z","article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","publication_identifier":{"eissn":["1477-9137"],"issn":["0021-9533"]},"type":"journal_article","status":"public","oa_version":"Published Version","article_number":"jcs264420","scopus_import":"1","citation":{"ieee":"V. Goeschl <i>et al.</i>, “α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures,” <i>Journal of Cell Science</i>, vol. 139, no. 8. The Company of Biologists, 2026.","ama":"Goeschl V, Hotka M, Hochreiter B, et al. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. <i>Journal of Cell Science</i>. 2026;139(8). doi:<a href=\"https://doi.org/10.1242/jcs.264420\">10.1242/jcs.264420</a>","chicago":"Goeschl, Vanessa, Matej Hotka, Bernhard Hochreiter, Karlheinz Hilber, Stefan Boehm, Andrey V. Kozlov, and Helmut Kubista. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary Cultures.” <i>Journal of Cell Science</i>. The Company of Biologists, 2026. <a href=\"https://doi.org/10.1242/jcs.264420\">https://doi.org/10.1242/jcs.264420</a>.","apa":"Goeschl, V., Hotka, M., Hochreiter, B., Hilber, K., Boehm, S., Kozlov, A. V., &#38; Kubista, H. (2026). α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.264420\">https://doi.org/10.1242/jcs.264420</a>","short":"V. Goeschl, M. Hotka, B. Hochreiter, K. Hilber, S. Boehm, A.V. Kozlov, H. Kubista, Journal of Cell Science 139 (2026).","ista":"Goeschl V, Hotka M, Hochreiter B, Hilber K, Boehm S, Kozlov AV, Kubista H. 2026. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. Journal of Cell Science. 139(8), jcs264420.","mla":"Goeschl, Vanessa, et al. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary Cultures.” <i>Journal of Cell Science</i>, vol. 139, no. 8, jcs264420, The Company of Biologists, 2026, doi:<a href=\"https://doi.org/10.1242/jcs.264420\">10.1242/jcs.264420</a>."},"abstract":[{"text":"Glutamate excitotoxicity is a cell death mechanism triggered by accumulation of glutamate in the extracellular space. The α-ketoglutarate dehydrogenase complex (αKGDHC), an enzyme of the tricarboxylic acid cycle, represents a branching point controlling glutamate formation and its consumption as a fuel. Hence, modulation of the activity of αKGDHC might alter the amount of glutamate available for excitotoxic effects. To address this hypothesis, hippocampal neurons in primary co-culture with glial cells were exposed to zero-Mg2 buffer to elicit excitotoxicity through N-methyl-D-aspartic acid (NMDA) receptor disinhibition. Pretreatment of the cultures with succinyl phosphonate, to inhibit αKGDHC, enhanced excitotoxity, whereas promotion of αKGDHC activity by pretreatment with thiamine caused an opposite action. Moreover, NMDA receptor currents – but not those mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors – were potentiated in neurons with impaired αKGDHC activity and diminished in neurons with boosted αKGDHC activity. The sensitization of NMDA receptors involved mGluR1 activation and was accompanied by enhanced neuronal discharge activity, elevated basal cytosolic Ca2+ levels, and augmented Ca2+ responses evoked by glutamate application. These results suggest that mGluR1-mediated potentiation of NMDA receptors contributes to a mechanism by which inhibition of αKGDHC might exacerbate glutamate excitotoxicity.","lang":"eng"}],"publisher":"The Company of Biologists","file_date_updated":"2026-05-12T06:27:54Z","author":[{"first_name":"Vanessa","last_name":"Goeschl","full_name":"Goeschl, Vanessa"},{"full_name":"Hotka, Matej","last_name":"Hotka","first_name":"Matej"},{"full_name":"Hochreiter, Bernhard","id":"e6cab3de-17f6-11ed-9210-c1e42e045e9d","first_name":"Bernhard","last_name":"Hochreiter"},{"full_name":"Hilber, Karlheinz","first_name":"Karlheinz","last_name":"Hilber"},{"last_name":"Boehm","first_name":"Stefan","full_name":"Boehm, Stefan"},{"full_name":"Kozlov, Andrey V.","first_name":"Andrey V.","last_name":"Kozlov"},{"full_name":"Kubista, Helmut","first_name":"Helmut","last_name":"Kubista"}],"language":[{"iso":"eng"}],"ddc":["570"],"doi":"10.1242/jcs.264420","file":[{"file_id":"21861","success":1,"date_created":"2026-05-12T06:27:54Z","access_level":"open_access","file_name":"2026_JourCellScience_Goeschl.pdf","creator":"dernst","checksum":"8db35c97588c2f6ef88c7e8d5924cf8c","content_type":"application/pdf","date_updated":"2026-05-12T06:27:54Z","relation":"main_file","file_size":1957057}],"publication_status":"published","oa":1,"intvolume":"       139","year":"2026","date_created":"2026-05-11T10:52:27Z","article_type":"original","day":"27","OA_place":"publisher","publication":"Journal of Cell Science","issue":"8","department":[{"_id":"Bio"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21860","pmid":1,"has_accepted_license":"1","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}},{"date_updated":"2026-06-10T07:49:04Z","quality_controlled":"1","external_id":{"pmid":["42088272"]},"month":"04","volume":17,"acknowledgement":"The computational results of this work have been achieved using the Life Science Compute Cluster (LiSC) of the University of Vienna. We additionally thank Julia Ramesmayer for assistance during DNA extraction and sample preparation for long-read sequencing. Support from the Mass Spectrometry Centre of the Faculty of Chemistry, University of Vienna, is thankfully acknowledged.\r\nThe author(s) declared that financial support was received for this work and/or its publication. This work was supported by the University of Vienna via the Research Platform Secondary Metabolomes of Bacterial Communities (MetaBac). Open access funding provided by University of Vienna. ","date_published":"2026-04-20T00:00:00Z","article_processing_charge":"Yes","PlanS_conform":"1","title":"Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments","OA_type":"gold","status":"public","publication_identifier":{"issn":["1664-302X"]},"type":"journal_article","oa_version":"Published Version","abstract":[{"text":"Several Streptomyces strains were isolated from freshwater sediments collected in the Laxenburg ponds (Lower Austria). Genome sequencing and bioinformatics analyses revealed biosynthetic gene clusters (BGCs) that may specify production of chemically diverse secondary metabolites. Various culture conditions were employed to induce metabolite production, and subsequent LC-MS analyses facilitated the identification of the produced compounds and their correlation with the corresponding BGCs. These analyses of sediment-derived Streptomyces spp. highlight their extensive biosynthetic potential, revealing a diverse range of bioactive secondary metabolites, including siderophores, antibiotics, and other compounds with potential therapeutic applications. Genomes of two Streptomyces isolates, one of them representing a potentially new species, harbored several uncharacterized BGCs that may specify biosynthesis of novel secondary metabolites. Although targeted overexpression of pathway-specific regulators from these BGCs did not yield additional metabolites, whereas knockout experiments led to metabolic changes, presumably reflecting regulatory or compensatory interactions between multiple biosynthetic pathways. Continued exploration of these strains and their BGCs may lead to the discovery of new bioactive molecules with pharmaceutical and biotechnological applications.","lang":"eng"}],"article_number":"1793713","citation":{"chicago":"Tocino-Márquez, Inmaculada, Martin Zehl, Jovana Batajic, Joana Séneca, Petra Pjevac, José Murillo-Alba, Jesús Martín, Olga N. Sekurova, and Sergey B. Zotchev. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp. from Freshwater Sediments.” <i>Frontiers in Microbiology</i>. Frontiers Media, 2026. <a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">https://doi.org/10.3389/fmicb.2026.1793713</a>.","apa":"Tocino-Márquez, I., Zehl, M., Batajic, J., Séneca, J., Pjevac, P., Murillo-Alba, J., … Zotchev, S. B. (2026). Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. <i>Frontiers in Microbiology</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">https://doi.org/10.3389/fmicb.2026.1793713</a>","ista":"Tocino-Márquez I, Zehl M, Batajic J, Séneca J, Pjevac P, Murillo-Alba J, Martín J, Sekurova ON, Zotchev SB. 2026. Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology. 17, 1793713.","short":"I. Tocino-Márquez, M. Zehl, J. Batajic, J. Séneca, P. Pjevac, J. Murillo-Alba, J. Martín, O.N. Sekurova, S.B. Zotchev, Frontiers in Microbiology 17 (2026).","mla":"Tocino-Márquez, Inmaculada, et al. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp. from Freshwater Sediments.” <i>Frontiers in Microbiology</i>, vol. 17, 1793713, Frontiers Media, 2026, doi:<a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">10.3389/fmicb.2026.1793713</a>.","ieee":"I. Tocino-Márquez <i>et al.</i>, “Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments,” <i>Frontiers in Microbiology</i>, vol. 17. Frontiers Media, 2026.","ama":"Tocino-Márquez I, Zehl M, Batajic J, et al. Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. <i>Frontiers in Microbiology</i>. 2026;17. doi:<a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">10.3389/fmicb.2026.1793713</a>"},"publisher":"Frontiers Media","author":[{"full_name":"Tocino-Márquez, Inmaculada","last_name":"Tocino-Márquez","first_name":"Inmaculada"},{"last_name":"Zehl","first_name":"Martin","orcid":"0000-0001-9685-0373","id":"8e016d5b-5d77-11f0-86d2-96cdb3922a55","full_name":"Zehl, Martin"},{"full_name":"Batajic, Jovana","first_name":"Jovana","last_name":"Batajic"},{"full_name":"Séneca, Joana","first_name":"Joana","last_name":"Séneca"},{"full_name":"Pjevac, Petra","first_name":"Petra","last_name":"Pjevac"},{"full_name":"Murillo-Alba, José","first_name":"José","last_name":"Murillo-Alba"},{"full_name":"Martín, Jesús","first_name":"Jesús","last_name":"Martín"},{"full_name":"Sekurova, Olga N.","last_name":"Sekurova","first_name":"Olga N."},{"first_name":"Sergey B.","last_name":"Zotchev","full_name":"Zotchev, Sergey B."}],"language":[{"iso":"eng"}],"ddc":["572"],"file_date_updated":"2026-06-10T07:46:30Z","intvolume":"        17","doi":"10.3389/fmicb.2026.1793713","file":[{"creator":"dernst","checksum":"31fb6b98c8a6d4007cb21808c6d2d9e3","access_level":"open_access","file_name":"2026_FrontiersMicrobiology_TocinoMarquez.pdf","date_created":"2026-06-10T07:46:30Z","file_id":"21989","success":1,"file_size":3582644,"date_updated":"2026-06-10T07:46:30Z","content_type":"application/pdf","relation":"main_file"}],"publication_status":"published","oa":1,"DOAJ_listed":"1","year":"2026","article_type":"original","date_created":"2026-06-08T08:34:10Z","day":"20","publication":"Frontiers in Microbiology","OA_place":"publisher","pmid":1,"department":[{"_id":"MassSpec"}],"_id":"21953","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}},{"author":[{"full_name":"Miranda, Osvaldo","id":"862A3C56-A8BF-11E9-B4FA-D9E3E5697425","orcid":"0000-0001-6618-6889","first_name":"Osvaldo","last_name":"Miranda"},{"id":"475990FE-F248-11E8-B48F-1D18A9856A87","full_name":"Contreras, Ximena","last_name":"Contreras","first_name":"Ximena"},{"full_name":"Pauler, Florian","id":"48EA0138-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7462-0048","last_name":"Pauler","first_name":"Florian"},{"last_name":"Davaatseren","first_name":"Amarbayasgalan","full_name":"Davaatseren, Amarbayasgalan","id":"70ADC922-B424-11E9-99E3-BA18E6697425"},{"first_name":"Nicole","last_name":"Amberg","orcid":"0000-0002-3183-8207","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","full_name":"Amberg, Nicole"},{"id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","full_name":"Streicher, Carmen","first_name":"Carmen","last_name":"Streicher"},{"id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","full_name":"Villalba Requena, Ana","first_name":"Ana","last_name":"Villalba Requena","orcid":"0000-0002-5615-5277"},{"full_name":"Heger, Anna-Magdalena","id":"4B76FFD2-F248-11E8-B48F-1D18A9856A87","last_name":"Heger","first_name":"Anna-Magdalena"},{"full_name":"Marie, Corentine","last_name":"Marie","first_name":"Corentine"},{"full_name":"Hassan, Bassem A.","last_name":"Hassan","first_name":"Bassem A."},{"full_name":"Rülicke, Thomas","last_name":"Rülicke","first_name":"Thomas"},{"orcid":"0000-0003-2279-1061","last_name":"Hippenmeyer","first_name":"Simon","full_name":"Hippenmeyer, Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87"}],"ddc":["570"],"language":[{"iso":"eng"}],"project":[{"grant_number":"F7805","_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression"},{"_id":"260018B0-B435-11E9-9278-68D0E5697425","grant_number":"725780","name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","call_identifier":"H2020"}],"doi":"10.64898/2026.05.01.722191","oa":1,"publication_status":"submitted","year":"2026","date_created":"2026-06-09T08:08:53Z","day":"05","publication":"bioRxiv","OA_place":"repository","corr_author":"1","department":[{"_id":"SiHi"},{"_id":"PreCl"},{"_id":"GradSch"}],"ec_funded":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21963","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)"},"date_updated":"2026-06-16T08:57:20Z","license":"https://creativecommons.org/licenses/by-nc/4.0/","month":"05","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"acknowledgement":"We thank Kay-Uwe Wagner (Wayne State University) for generously sharing Jak1/2–flox mouse lines; A.\r\nSommer (VBCF GmbH, NGS Unit) for technical support; N. Kim, V. Mick, S. Schnabl, S. Gobeil, and L.\r\nAndersen for technical assistance; all members of the Hippenmeyer lab for discussion and B. Novitch for\r\ncomments on earlier versions of the manuscript. This research was supported by the Scientific Service Units\r\n(SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). O.A.M received support from the Austrian Academy of Sciences\r\nÖAW (DOC 186584), and N.A. from FWF Elise Richter Program (Grant V1041T). This work was also\r\nsupported by IST Austria institutional funds; FWF SFB F78 (Neuro Stem Modulation) to S.H., and the\r\nEuropean Research Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogramme (grant agreement No 725780 LinPro) to S.H.","date_published":"2026-05-05T00:00:00Z","article_processing_charge":"No","title":"Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production","OA_type":"green","status":"public","type":"preprint","oa_version":"Preprint","citation":{"apa":"Miranda, O., Contreras, X., Pauler, F., Davaatseren, A., Amberg, N., Streicher, C., … Hippenmeyer, S. (n.d.). Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.05.01.722191\">https://doi.org/10.64898/2026.05.01.722191</a>","chicago":"Miranda, Osvaldo, Ximena Contreras, Florian Pauler, Amarbayasgalan Davaatseren, Nicole Amberg, Carmen Streicher, Ana Villalba Requena, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.05.01.722191\">https://doi.org/10.64898/2026.05.01.722191</a>.","mla":"Miranda, Osvaldo, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>.","short":"O. Miranda, X. Contreras, F. Pauler, A. Davaatseren, N. Amberg, C. Streicher, A. Villalba Requena, A.-M. Heger, C. Marie, B.A. Hassan, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.).","ista":"Miranda O, Contreras X, Pauler F, Davaatseren A, Amberg N, Streicher C, Villalba Requena A, Heger A-M, Marie C, Hassan BA, Rülicke T, Hippenmeyer S. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv, <a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>.","ieee":"O. Miranda <i>et al.</i>, “Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production,” <i>bioRxiv</i>. .","ama":"Miranda O, Contreras X, Pauler F, et al. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>"},"abstract":[{"text":"The cerebral cortex consists of immense numbers of neuronal and glial cell-types derived from radial glial progenitor (RGP) cells. How RGPs generate appropriate quantities of distinct cortical cell-types to safeguard a brain of correct size, is not well understood. However, genetic aberration in human, including mutations in PTEN, lead to cortical malformation such as macrocephaly, albeit with unknown etiology. Here we utilized Mosaic Analysis with Double Markers (MADM)-based clonal analysis and single cell phenotyping to decipher the role of Pten in neurogenic and gliogenic RGP lineage progression during cortical ontogeny. While neurogenic RGP lineage progression and projection neuron production was moderately altered in the absence of Pten, cortical astrocyte production was drastically increased. Through genetic epistasis experiments we show that the loss of Pten uncouples astrocyte generation from essential growth factor signaling hubs, funneling into MAPK. Collectively, our results suggest that Pten regulates RGP lineage progression with distinct sequential functions in cortical projection neurogenesis and astrocyte production to ensure the emergence of a correctly-sized cerebral cortex.","lang":"eng"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.64898/2026.05.01.722191"}]},{"has_accepted_license":"1","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"pmid":1,"ec_funded":1,"department":[{"_id":"PeJo"},{"_id":"ScienComp"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"22229","publication":"Nature Communications","OA_place":"publisher","corr_author":"1","researchdata_availability":"yes","day":"23","article_type":"original","date_created":"2026-06-30T13:05:52Z","DOAJ_listed":"1","year":"2026","intvolume":"        17","project":[{"name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","grant_number":"692692"},{"name":"Synaptic mechanisms of engram storage and retrieval in CA3 hippocampal microcircuits","grant_number":"101199096","_id":"e62b56fe-ab3c-11f0-94c7-d181dd352b3b"},{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"name":"Synaptic computations of the hippocampal CA3 circuitry","call_identifier":"H2020","grant_number":"101026635","_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9"},{"name":"Mechanisms of GABA release in hippocampal circuits","_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","grant_number":"P36232"},{"name":"Synaptic networks of human brain","_id":"8d9195e9-16d5-11f0-9cad-d075be887a1e","grant_number":"PAT 4178023"},{"name":"Reglas de Conectividad funcional en el hipocampo","_id":"26366136-B435-11E9-9278-68D0E5697425"}],"file":[{"date_created":"2026-07-01T06:46:06Z","success":1,"file_id":"22231","checksum":"d0b0093493926985b4c268662ff4d556","creator":"dernst","file_name":"2026_NatureComm_VargasBarroso.pdf","access_level":"open_access","file_size":18304997,"relation":"main_file","content_type":"application/pdf","date_updated":"2026-07-01T06:46:06Z"}],"doi":"10.1038/s41467-026-71914-x","oa":1,"publication_status":"published","author":[{"id":"2F55A9DE-F248-11E8-B48F-1D18A9856A87","full_name":"Vargas Barroso, Victor M","first_name":"Victor M","last_name":"Vargas Barroso"},{"last_name":"Watson","first_name":"Jake","orcid":"0000-0002-8698-3823","id":"63836096-4690-11EA-BD4E-32803DDC885E","full_name":"Watson, Jake"},{"full_name":"Navas Olivé, Andrea C","id":"739d26c9-52e8-11ee-8d72-f14d3893b4ce","orcid":"0000-0002-9280-8597","last_name":"Navas Olivé","first_name":"Andrea C"},{"orcid":"0000-0002-5621-8100","first_name":"Alois","last_name":"Schlögl","full_name":"Schlögl, Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-5001-4804","last_name":"Jonas","first_name":"Peter M","full_name":"Jonas, Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"language":[{"iso":"eng"}],"ddc":["570"],"dataavailabilitystatement":"Source data are provided with this paper. Additional original data are available from the corresponding author upon request. Code is available from https://doi.org/10.15479/AT-ISTA-21442 under the link https://research-explorer.ista.ac.at/download/21442/21443/ca3simu-vargas2026v1.tar.gz","file_date_updated":"2026-07-01T06:46:06Z","das_tickbox":"1","publisher":"Springer Nature","oa_version":"Published Version","scopus_import":"1","citation":{"ama":"Vargas Barroso VM, Watson J, Navas Olivé AC, Schlögl A, Jonas PM. Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-71914-x\">10.1038/s41467-026-71914-x</a>","ieee":"V. M. Vargas Barroso, J. Watson, A. C. Navas Olivé, A. Schlögl, and P. M. Jonas, “Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026.","mla":"Vargas Barroso, Victor M., et al. “Developmental Emergence of Sparse and Structured Synaptic Connectivity in the Hippocampal CA3 Memory Circuit.” <i>Nature Communications</i>, vol. 17, 5540, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-71914-x\">10.1038/s41467-026-71914-x</a>.","ista":"Vargas Barroso VM, Watson J, Navas Olivé AC, Schlögl A, Jonas PM. 2026. Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. Nature Communications. 17, 5540.","short":"V.M. Vargas Barroso, J. Watson, A.C. Navas Olivé, A. Schlögl, P.M. Jonas, Nature Communications 17 (2026).","apa":"Vargas Barroso, V. M., Watson, J., Navas Olivé, A. C., Schlögl, A., &#38; Jonas, P. M. (2026). Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-71914-x\">https://doi.org/10.1038/s41467-026-71914-x</a>","chicago":"Vargas Barroso, Victor M, Jake Watson, Andrea C Navas Olivé, Alois Schlögl, and Peter M Jonas. “Developmental Emergence of Sparse and Structured Synaptic Connectivity in the Hippocampal CA3 Memory Circuit.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-71914-x\">https://doi.org/10.1038/s41467-026-71914-x</a>."},"article_number":"5540","abstract":[{"text":"Hippocampal CA3 pyramidal neurons (PNs) form the largest autoassociative network in the mammalian brain. Whether CA3–CA3 recurrent connectivity is genetically preconfigured or environmentally shaped during ongoing memory storage is currently unknown. To address this question, we performed multicellular patch-clamp-based circuit mapping of up to eight CA3 PNs in the mouse hippocampus at multiple postnatal time points (P7–8, P18–25, and P45–50). Here, we show that the hippocampal CA3 network undergoes a developmental transformation from local, dense, and random connectivity to a distributed, sparse, and structured configuration. Thus, sparse and structured connectivity may emerge via experience-dependent mechanisms. In parallel, the strength of single synapses is downregulated; single synaptic events are sufficient to trigger postsynaptic spiking early in development, whereas spatial summation of several inputs is required at later time points. Biologically inspired models of memory storage by Hebbian synaptic plasticity and retrieval via pattern completion suggest that developmental changes improve specific aspects of memory storage and retrieval. Our results imply a developmental transformation of the neuronal code and the memory functions in the hippocampal CA3 network.</jats:p>","lang":"eng"}],"status":"public","publication_identifier":{"eissn":["2041-1723"]},"type":"journal_article","supplementarymaterial":"yes","OA_type":"gold","related_material":{"record":[{"status":"public","relation":"research_data","id":"21442"}]},"acknowledgement":"We thank Jose Guzman, Simon Hippenmeyer, and Tim Vogels for critically reading the manuscript, Jozsef Csicsvari for useful discussions, Florian Marr for technical assistance, and Eleftheria Kralli-Beller for manuscript editing. This research was supported by the Scientific Services Units (SSUs) of ISTA: the preclinical facility (PCF) provided housing and breeding of the animals, the imaging and optics facility (IOF) offered technical training and state of the art equipment, the Miba machine shop contributed to the construction and maintenance of multicellular recording setups, and the scientific computing unit helped with the large-scale simulations. The project received funding from the European Union’s Horizon 2020 research and innovation programme (ERC Advanced Grants No 692692 GIANTSYN and 101199096 CA3-SYNGRAM to P.J.; Marie Skłodowska-Curie Grant 754411 to V.V.B.; Marie Skłodowska-Curie Grant 101026635 to J.F.W.), the Fond zur Förderung der Wissenschaftlichen Forschung (P 36232-B, PAT4178023, and 10.55776/CoE16 to P.J.), and the Nomis Foundation (fellowship to A.N.-O.). V.V.B. received funding from a CONACyT fellowship (289638).","article_processing_charge":"Yes","date_published":"2026-06-23T00:00:00Z","PlanS_conform":"1","title":"Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"M-Shop"},{"_id":"ScienComp"}],"volume":17,"month":"06","quality_controlled":"1","date_updated":"2026-07-01T06:47:49Z","external_id":{"pmid":["42014695"]}},{"file_date_updated":"2026-03-12T10:24:45Z","author":[{"first_name":"Alois","last_name":"Schlögl","orcid":"0000-0002-5621-8100","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","full_name":"Schlögl, Alois"}],"date_updated":"2026-07-01T06:47:49Z","license":"https://opensource.org/licenses/GPL-3.0","doi":"10.15479/AT-ISTA-21442","file":[{"file_size":160410,"date_updated":"2026-03-12T08:19:14Z","relation":"main_file","content_type":"application/gzip","checksum":"441c8827717dcda05f91c127d15cf1e9","creator":"schloegl","access_level":"open_access","file_name":"ca3simu-vargas2026v1.tar.gz","date_created":"2026-03-12T08:19:14Z","file_id":"21443","success":1},{"relation":"main_file","date_updated":"2026-03-12T10:24:45Z","content_type":"text/markdown","file_size":10923,"success":1,"file_id":"21445","date_created":"2026-03-12T10:24:45Z","file_name":"README.md","access_level":"open_access","creator":"schloegl","checksum":"3c0092076228a15c0a7ae703192d43ea"}],"month":"03","project":[{"name":"Synaptic mechanisms of engram storage and retrieval in CA3 hippocampal microcircuits","_id":"e62b56fe-ab3c-11f0-94c7-d181dd352b3b","grant_number":"101199096"},{"name":"Mechanisms of GABA release in hippocampal circuits","grant_number":"P36232","_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5"},{"grant_number":"PAT 4178023","_id":"8d9195e9-16d5-11f0-9cad-d075be887a1e","name":"Synaptic networks of human brain"},{"name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","call_identifier":"H2020","grant_number":"692692","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"}],"oa":1,"year":"2026","date_created":"2026-03-12T08:20:46Z","title":"CA3Simu v1.06 (vargas2026v1)","date_published":"2026-03-12T00:00:00Z","related_material":{"record":[{"relation":"used_in_publication","id":"22229","status":"public"}]},"day":"12","corr_author":"1","type":"software","status":"public","department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"ec_funded":1,"user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","_id":"21442","citation":{"ama":"Schlögl A. CA3Simu v1.06 (vargas2026v1). 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21442\">10.15479/AT-ISTA-21442</a>","ieee":"A. Schlögl, “CA3Simu v1.06 (vargas2026v1).” Institute of Science and Technology Austria, 2026.","short":"A. Schlögl, (2026).","ista":"Schlögl A. 2026. CA3Simu v1.06 (vargas2026v1), Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT-ISTA-21442\">10.15479/AT-ISTA-21442</a>.","mla":"Schlögl, Alois. <i>CA3Simu v1.06 (Vargas2026v1)</i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-21442\">10.15479/AT-ISTA-21442</a>.","chicago":"Schlögl, Alois. “CA3Simu v1.06 (Vargas2026v1).” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-21442\">https://doi.org/10.15479/AT-ISTA-21442</a>.","apa":"Schlögl, A. (2026). CA3Simu v1.06 (vargas2026v1). Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-21442\">https://doi.org/10.15479/AT-ISTA-21442</a>"},"keyword":["hypocampus","ca3 simulations","modelling"],"publisher":"Institute of Science and Technology Austria","has_accepted_license":"1","tmp":{"short":"GPL 3.0","name":"GNU General Public License 3.0","legal_code_url":"https://www.gnu.org/licenses/gpl-3.0.en.html"}},{"status":"public","type":"journal_article","publication_identifier":{"eissn":["1751-7915"]},"OA_type":"gold","supplementarymaterial":"yes","das_tickbox":"1","publisher":"Wiley","citation":{"ieee":"S. Kralova <i>et al.</i>, “Kineochelins - A new group of siderophores from an antarctic bacterium,” <i>Microbial Biotechnology</i>, vol. 19, no. 6. Wiley, 2026.","ama":"Kralova S, Spacek P, Gafriller J, et al. Kineochelins - A new group of siderophores from an antarctic bacterium. <i>Microbial Biotechnology</i>. 2026;19(6). doi:<a href=\"https://doi.org/10.1111/1751-7915.70386\">10.1111/1751-7915.70386</a>","apa":"Kralova, S., Spacek, P., Gafriller, J., Bezdicek, M., Medvedcova, V., Séneca, J., … Loy, A. (2026). Kineochelins - A new group of siderophores from an antarctic bacterium. <i>Microbial Biotechnology</i>. Wiley. <a href=\"https://doi.org/10.1111/1751-7915.70386\">https://doi.org/10.1111/1751-7915.70386</a>","chicago":"Kralova, Stanislava, Peter Spacek, Johannes Gafriller, Matej Bezdicek, Viktoria Medvedcova, Joana Séneca, Jay Osvatic, et al. “Kineochelins - A New Group of Siderophores from an Antarctic Bacterium.” <i>Microbial Biotechnology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/1751-7915.70386\">https://doi.org/10.1111/1751-7915.70386</a>.","mla":"Kralova, Stanislava, et al. “Kineochelins - A New Group of Siderophores from an Antarctic Bacterium.” <i>Microbial Biotechnology</i>, vol. 19, no. 6, e70386, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/1751-7915.70386\">10.1111/1751-7915.70386</a>.","ista":"Kralova S, Spacek P, Gafriller J, Bezdicek M, Medvedcova V, Séneca J, Osvatic J, Grienke U, Rattei T, Sekurova ON, Zotchev SB, Zehl M, Loy A. 2026. Kineochelins - A new group of siderophores from an antarctic bacterium. Microbial Biotechnology. 19(6), e70386.","short":"S. Kralova, P. Spacek, J. Gafriller, M. Bezdicek, V. Medvedcova, J. Séneca, J. Osvatic, U. Grienke, T. Rattei, O.N. Sekurova, S.B. Zotchev, M. Zehl, A. Loy, Microbial Biotechnology 19 (2026)."},"article_number":"e70386","abstract":[{"lang":"eng","text":"The global rise of antimicrobial resistance has intensified the search for new microbial metabolites from underexplored environments and taxonomic groups. Extreme and geographically isolated habitats such as Antarctic terrestrial ecosystems represent promising reservoirs of biosynthetic diversity, particularly among rare and difficult-to-cultivate actinomycetes that may produce chemically diverse metabolites with potential biotechnological applications. Here, we report the characterization of kineochelins, a previously undescribed group of siderophores produced by the Antarctic isolate Actinokineospora sp. UV203, representing a difficult-to-cultivate actinomycete lineage. Structural elucidation revealed a set of closely related congeners with a mixed-ligand architecture consistent with metal-chelating activity. Genome mining combined with transcriptomic analysis identified a dedicated nonribosomal peptide synthetase-encoding biosynthetic gene cluster responsible for kineochelin production. Comparative genomic analyses indicated that, although kineochelin biosynthetic genes share limited similarity with known mixed-ligand siderophores, their gene content and organization differ substantially, suggesting a distinct biosynthetic lineage. Functional characterization of the culture supernatant and an enriched pre-purified kineochelin fraction demonstrated strong and selective iron chelation, with high affinity for ferric and ferrous iron. Crude culture extracts inhibited the growth of bacterial strains isolated from the same Antarctic environment, indicating that kineochelins may contribute to iron-mediated microbial competition. In addition, kineochelin-enriched pre-purified fractions showed moderate selective inhibitory activity against the opportunistic yeast pathogen Nakaseomyces glabratus and a clinical isolate of Saccharomyces cerevisiae associated with invasive infection. These findings expand the chemical and biosynthetic diversity known within the genus Actinokineospora and demonstrate that Antarctic rare actinomycetes represent valuable sources of previously unexplored natural products. The discovery of kineochelins highlights the potential of genome-guided exploration of polar microorganisms for identifying bioactive metabolites with relevance for antimicrobial discovery and biotechnology."}],"keyword":["Actinokineospora","Antarctica","antimicrobial discovery","biosynthetic gene cluster","genome mining","microbial competition","nonribosomalpeptide synthetase","siderophores"],"scopus_import":"1","oa_version":"Published Version","month":"06","quality_controlled":"1","date_updated":"2026-07-13T06:59:08Z","external_id":{"pmid":["42210522"],"biorxivid":["10.64898/2026.02.23.707395"]},"article_processing_charge":"Yes","date_published":"2026-06-01T00:00:00Z","acknowledgement":"This work was supported by the Czech Antarctic Research Programme 2025–2027 (VAN 2025) and the University of Vienna via the Research Platform Secondary Metabolomes of Bacterial Communities (MetaBac). S.K. has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 101020356 (DEFCOMANT, https://doi.org/10.3030/101020356) and MASH StG/CoG (MUNI/SC/1946/2024) by Masaryk University. T.R. and A.L. were funded in part by the Austrian Science Fund FWF [grant DOI https://doi.org/10.55776/COE7]. M.B. was funded by the Ministry of Health, Czech Republic—conceptual development of research organization (FNBr, 65269705). The Life Science Compute Cluster LiSC at the University of Vienna provided the high-performance computing infrastructure for this study. We thank Julia Ramesmayer and Sara Malinowski (Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna) for assistance during high molecular weight extraction and RNA extraction. The authors thank Anna Fabisikova and Michael Klemm-Abraham from the Mass Spectrometry Centre and the team of the NMR Centre (both core facilities of the Faculty of Chemistry, University of Vienna, and members of the Vienna Life Science Instruments) for assistance with data acquisition. We are thankful to Dr. Jaime Felipe Guerrero Garzón for helpful discussions on the use of a rrn operon promoter strategy. For open access purposes, the authors have applied for a CC BY public copyright licence to any author-accepted manuscript version arising from this submission. Dr. Martin Kello (Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia) and Dr. Michal Goga (Department of Plant Biology, Faculty of Science and Center for Interdisciplinary Biosciences, Technology and Innovation Park, Pavol Jozef Šafárik University in Košice, Košice, Slovakia), funded by VEGA 1/0498/23, are acknowledged for their assistance with the antiproliferative assays. This work was supported by Horizon 2020 Framework Programme, 101020356; Universität Wien, MetaBac; Ministry of Education, Youth and Sports, VAN 2025; Masarykova Univerzita, MUNI/SC/1946/2024; Austrian Science Fund, 10.55776/COE7; Ministerstvo Zdravotnictví České Republiky, FNBr, 65269705; Vedecká grantová agentúra Ministerstva školstva, výskumu, vývoja a mládeže Slovenskej republiky a Slovenskej akadémie vied, VEGA 1/0498/23.","title":"Kineochelins - A new group of siderophores from an antarctic bacterium","biorxivid":1,"volume":19,"publication":"Microbial Biotechnology","corr_author":"1","OA_place":"publisher","day":"01","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"22254","department":[{"_id":"MassSpec"}],"issue":"6","intvolume":"        19","publication_status":"published","oa":1,"doi":"10.1111/1751-7915.70386","file":[{"access_level":"open_access","file_name":"2026_MicrobialBiotechnology_Kralova.pdf","checksum":"4f735714644f1049b22b014225843d8d","creator":"dernst","file_id":"22271","success":1,"date_created":"2026-07-13T06:57:19Z","content_type":"application/pdf","relation":"main_file","date_updated":"2026-07-13T06:57:19Z","file_size":2497486}],"ddc":["570"],"language":[{"iso":"eng"}],"author":[{"last_name":"Kralova","first_name":"Stanislava","full_name":"Kralova, Stanislava"},{"full_name":"Spacek, Peter","last_name":"Spacek","first_name":"Peter"},{"full_name":"Gafriller, Johannes","last_name":"Gafriller","first_name":"Johannes"},{"full_name":"Bezdicek, Matej","first_name":"Matej","last_name":"Bezdicek"},{"first_name":"Viktoria","last_name":"Medvedcova","full_name":"Medvedcova, Viktoria"},{"full_name":"Séneca, Joana","last_name":"Séneca","first_name":"Joana"},{"first_name":"Jay","last_name":"Osvatic","full_name":"Osvatic, Jay"},{"last_name":"Grienke","first_name":"Ulrike","full_name":"Grienke, Ulrike"},{"last_name":"Rattei","first_name":"Thomas","full_name":"Rattei, Thomas"},{"first_name":"Olga N.","last_name":"Sekurova","full_name":"Sekurova, Olga N."},{"first_name":"Sergey B.","last_name":"Zotchev","full_name":"Zotchev, Sergey B."},{"orcid":"0000-0001-9685-0373","last_name":"Zehl","first_name":"Martin","full_name":"Zehl, Martin","id":"8e016d5b-5d77-11f0-86d2-96cdb3922a55"},{"last_name":"Loy","first_name":"Alexander","full_name":"Loy, Alexander"}],"dataavailabilitystatement":"The genome sequence and transcriptomic data of strain Actinokineospora sp. UV203 are available on NCBI (BioProject accession number PRJNA1331526). The nearly full-length 16S rRNA gene (1395 bp) of strain Actinokineospora sp. UV203 is available on NCBI (accession number PX090945). The NMR data of kineochelin E1 and A1 are deposited in the Natural Products Magnetic Resonance Database (NP-MRD) under accession numbers NP0352113 and NP0352114, respectively.","file_date_updated":"2026-07-13T06:57:19Z","article_type":"original","date_created":"2026-07-08T09:19:43Z","DOAJ_listed":"1","year":"2026"},{"abstract":[{"lang":"eng","text":"Single-stranded, helically folded aromatic oligoamides bearing anionic phosphonate side chains have been shown to bind to some DNA-binding proteins better than DNA itself. However, these DNA mimic foldamers have until now mainly consisted of a single repeat motif, like a poly(dA:dT) DNA duplex, and contained limited sequence information. Here, we introduce new monomers designed to display different chemical functionalities in the major groove of the DNA mimics. Four new Fmoc-protected amino acid monomers have been synthesized and incorporated into oligomers. Sixteen foldamer sequences were prepared on solid phase. Their conformations in solution and in the solid state and their conformational dynamics were investigated using nuclear magnetic resonance, circular dichroism, molecular modeling, and X-ray crystallography. The results show that three of the four new monomers behaved as designed and that their introduction enhances the conformational dynamics of the DNA mimic foldamers. In a fourth case, conformational behavior proved to be more complex than expected. The modified sequences retained the ability to bind to the bacterial histone-like protein HU. These results showcase design strategies to manipulate large molecular biomimetics in which not only side chains but also main chain components are varied. The new monomers pave the way to complex DNA mimic foldamer sequences targeting proteins that recognize sequence-selective DNA-binding proteins such as transcription factors or restriction enzymes."}],"citation":{"ista":"Wu J, Corvaglia V, Chakrabortty T, Mandal PK, Huc I. Tailoring the major groove of DNA mimic foldamers. Chemical Science.","short":"J. Wu, V. Corvaglia, T. Chakrabortty, P.K. Mandal, I. Huc, Chemical Science (n.d.).","mla":"Wu, Jiaojiao, et al. “Tailoring the Major Groove of DNA Mimic Foldamers.” <i>Chemical Science</i>, Royal Society of Chemistry, doi:<a href=\"https://doi.org/10.1039/d6sc00798h\">10.1039/d6sc00798h</a>.","chicago":"Wu, Jiaojiao, Valentina Corvaglia, Tulika Chakrabortty, Pradeep K Mandal, and Ivan Huc. “Tailoring the Major Groove of DNA Mimic Foldamers.” <i>Chemical Science</i>. Royal Society of Chemistry, n.d. <a href=\"https://doi.org/10.1039/d6sc00798h\">https://doi.org/10.1039/d6sc00798h</a>.","apa":"Wu, J., Corvaglia, V., Chakrabortty, T., Mandal, P. K., &#38; Huc, I. (n.d.). Tailoring the major groove of DNA mimic foldamers. <i>Chemical Science</i>. Royal Society of Chemistry. <a href=\"https://doi.org/10.1039/d6sc00798h\">https://doi.org/10.1039/d6sc00798h</a>","ama":"Wu J, Corvaglia V, Chakrabortty T, Mandal PK, Huc I. Tailoring the major groove of DNA mimic foldamers. <i>Chemical Science</i>. doi:<a href=\"https://doi.org/10.1039/d6sc00798h\">10.1039/d6sc00798h</a>","ieee":"J. Wu, V. Corvaglia, T. Chakrabortty, P. K. Mandal, and I. Huc, “Tailoring the major groove of DNA mimic foldamers,” <i>Chemical Science</i>. Royal Society of Chemistry."},"scopus_import":"1","oa_version":"Published Version","publisher":"Royal Society of Chemistry","das_tickbox":"1","OA_type":"gold","supplementarymaterial":"yes","type":"journal_article","publication_identifier":{"issn":["2041-6520"],"eissn":["2041-6539"]},"status":"public","title":"Tailoring the major groove of DNA mimic foldamers","article_processing_charge":"Yes","date_published":"2026-06-09T00:00:00Z","acknowledgement":"We acknowledge financial support from the European Research Council (ERC) under the European Union's Horizon Europe Framework Programme (grant agreement no. ERC-2021-ADG-320892) and from the China Scholarship Council (CSC, predoctoral fellowship to J. W.). We thank L. Allmendinger for assistance with NMR measurements, P. Mayer for his assistance in solving the crystal structures of 1 and 1d, L. Bodero for assistance with automated solid-phase synthesis, M. Rogovoi for providing monomer precursors, and M. Loos for the purification and analysis of compounds 15a–19a. We thank M. Soler-Lopez (ID23-1, ESRF, Grenoble) and I. Bento (EMBL P13, Petra III, DESY, Hamburg) for assistance during data collection at the synchrotron beamlines.","quality_controlled":"1","date_updated":"2026-07-13T11:24:29Z","month":"06","_id":"22289","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"LifeSc"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","day":"09","researchdata_availability":"yes","OA_place":"publisher","publication":"Chemical Science","year":"2026","DOAJ_listed":"1","date_created":"2026-07-13T09:41:36Z","article_type":"original","dataavailabilitystatement":"CCDC 2514117, 2514118, 2286782 and 2478322 (compound 1, compound 1d, oligomer 5, and oligomer 6, respectively) contain the supplementary crystallographic data for this paper.54a–d \r\n\r\nThe supporting data have been provided as part of the supplementary information (SI). Supplementary information: SI figures, detailed experimental protocols, crystallographic studies, and characterisation of new compounds. See DOI: https://doi.org/10.1039/d6sc00798h.","ddc":["540"],"language":[{"iso":"eng"}],"author":[{"full_name":"Wu, Jiaojiao","first_name":"Jiaojiao","last_name":"Wu"},{"last_name":"Corvaglia","first_name":"Valentina","full_name":"Corvaglia, Valentina"},{"last_name":"Chakrabortty","first_name":"Tulika","full_name":"Chakrabortty, Tulika"},{"full_name":"Mandal, Pradeep K","id":"6a3def15-d4b4-11ef-9fa9-a24c1f545ec3","orcid":"0000-0001-5996-956X","last_name":"Mandal","first_name":"Pradeep K"},{"full_name":"Huc, Ivan","first_name":"Ivan","last_name":"Huc"}],"publication_status":"inpress","doi":"10.1039/d6sc00798h"},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"PlanS_conform":"1","title":"Auxin response and PIN‐mediated transport in chlorophyte algae","date_published":"2026-06-10T00:00:00Z","article_processing_charge":"Yes (via OA deal)","acknowledgement":"Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria.","external_id":{"pmid":["42271607"]},"quality_controlled":"1","date_updated":"2026-07-13T14:26:31Z","month":"06","article_number":"jipb.70309","citation":{"chicago":"Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>.","apa":"Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>","ista":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309.","short":"A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026).","mla":"Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>, jipb. 70309, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>.","ieee":"A. Smoljan <i>et al.</i>, “Auxin response and PIN‐mediated transport in chlorophyte algae,” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026.","ama":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. 2026. doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>"},"abstract":[{"text":"Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage.","lang":"eng"}],"scopus_import":"1","oa_version":"Published Version","publisher":"Wiley","main_file_link":[{"url":"https://doi.org/10.1111/jipb.70309","open_access":"1"}],"das_tickbox":"0","OA_type":"hybrid","supplementarymaterial":"yes","type":"journal_article","publication_identifier":{"eissn":["1744-7909"],"issn":["1672-9072"]},"status":"public","year":"2026","date_created":"2026-07-13T10:44:55Z","article_type":"original","ddc":["580"],"language":[{"iso":"eng"}],"author":[{"full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","first_name":"Adrijana","last_name":"Smoljan"},{"last_name":"Koutnik‐Abele","first_name":"Sarah","full_name":"Koutnik‐Abele, Sarah"},{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii","last_name":"Vladimirtsev","first_name":"Dmitrii"},{"last_name":"Klíma","first_name":"Petr","full_name":"Klíma, Petr"},{"last_name":"Bírošíková","first_name":"Anita","full_name":"Bírošíková, Anita"},{"orcid":"0000-0003-2627-6956","first_name":"Yuzhou","last_name":"Zhang","full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack","orcid":"0000-0001-5145-4609"},{"last_name":"Schuster","first_name":"Maximilian","id":"37e65def-d415-11eb-ae59-a7b67be103db","full_name":"Schuster, Maximilian"},{"full_name":"Kurtović, Katarina","last_name":"Kurtović","first_name":"Katarina"},{"full_name":"Hammes, Ulrich Z.","first_name":"Ulrich Z.","last_name":"Hammes"},{"full_name":"Petrášek, Jan","last_name":"Petrášek","first_name":"Jan"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml"}],"oa":1,"publication_status":"epub_ahead","project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"doi":"10.1111/jipb.70309","_id":"22301","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"NanoFab"},{"_id":"Bio"}],"pmid":1,"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","day":"10","researchdata_availability":"no","OA_place":"publisher","corr_author":"1","publication":"Journal of Integrative Plant Biology"},{"PlanS_conform":"1","title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses","article_processing_charge":"Yes (in subscription journal)","date_published":"2026-02-11T00:00:00Z","acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","volume":61,"acknowledged_ssus":[{"_id":"NanoFab"}],"month":"02","external_id":{"pmid":["41192429"]},"date_updated":"2026-07-23T06:27:15Z","quality_controlled":"1","publisher":"Elsevier","das_tickbox":"1","abstract":[{"lang":"eng","text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo."}],"keyword":["thermobiology","cell migration","thermo-adaptability of immune cells"],"scopus_import":"1","citation":{"chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>.","apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>","short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61 (2026) 356–371.e12.","ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12.","mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>, vol. 61, no. 2, Elsevier, 2026, p. 356–371.e12, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>.","ieee":"I. Company-Garrido <i>et al.</i>, “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” <i>Developmental Cell</i>, vol. 61, no. 2. Elsevier, p. 356–371.e12, 2026.","ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. 2026;61(2):356-371.e12. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>"},"oa_version":"Published Version","type":"journal_article","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"status":"public","OA_type":"hybrid","supplementarymaterial":"yes","date_created":"2025-12-28T23:01:27Z","article_type":"original","year":"2026","publication_status":"published","oa":1,"file":[{"content_type":"application/pdf","date_updated":"2026-07-23T06:26:25Z","relation":"main_file","file_size":12342817,"file_name":"2026_DevelopmentalCell_CompanyGarrido.pdf","access_level":"open_access","creator":"dernst","checksum":"52fd52d2d19a4514f8fcc1b40f420ca2","success":1,"file_id":"22388","date_created":"2026-07-23T06:26:25Z"}],"doi":"10.1016/j.devcel.2025.10.006","intvolume":"        61","file_date_updated":"2026-07-23T06:26:25Z","dataavailabilitystatement":"This study did not generate new unique reagents. Data are available upon request.\r\n•The custom-made codes used in this study are available at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","ddc":["570"],"language":[{"iso":"eng"}],"author":[{"full_name":"Company-Garrido, Iván","last_name":"Company-Garrido","first_name":"Iván"},{"first_name":"Alberto","last_name":"Zurita Carpio","full_name":"Zurita Carpio, Alberto"},{"last_name":"Colomer-Rosell","first_name":"Mariona","full_name":"Colomer-Rosell, Mariona"},{"last_name":"Ciraulo","first_name":"Bernard","full_name":"Ciraulo, Bernard"},{"full_name":"Molkenbur, Ronja","first_name":"Ronja","last_name":"Molkenbur"},{"first_name":"Peter","last_name":"Lanzerstorfer","full_name":"Lanzerstorfer, Peter"},{"full_name":"Pezzano, Fabio","last_name":"Pezzano","first_name":"Fabio"},{"full_name":"Agazzi, Costanza","last_name":"Agazzi","first_name":"Costanza"},{"full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","last_name":"Hauschild","first_name":"Robert"},{"full_name":"Jain, Saumey","last_name":"Jain","first_name":"Saumey"},{"full_name":"Jacques, Jeroen M.","first_name":"Jeroen M.","last_name":"Jacques"},{"last_name":"Venturini","first_name":"Valeria","full_name":"Venturini, Valeria"},{"last_name":"Knapp","first_name":"Christian","full_name":"Knapp, Christian"},{"first_name":"Yufei","last_name":"Xie","full_name":"Xie, Yufei"},{"orcid":"0000-0001-5145-4609","first_name":"Jack","last_name":"Merrin","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Weghuber, Julian","last_name":"Weghuber","first_name":"Julian"},{"full_name":"Schaaf, Marcel","last_name":"Schaaf","first_name":"Marcel"},{"full_name":"Quidant, Romain","first_name":"Romain","last_name":"Quidant"},{"full_name":"Kiermaier, Eva","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6165-5738","last_name":"Kiermaier","first_name":"Eva"},{"first_name":"Jaime","last_name":"Ortega Arroyo","full_name":"Ortega Arroyo, Jaime"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","last_name":"Ruprecht","first_name":"Verena","orcid":"0000-0003-4088-8633"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan","first_name":"Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217"}],"page":"356-371.e12","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","_id":"20859","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"2","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"pmid":1,"OA_place":"publisher","publication":"Developmental Cell","day":"11","researchdata_availability":"upon request"},{"article_processing_charge":"Yes (via OA deal)","date_published":"2026-06-01T00:00:00Z","acknowledgement":"This work was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Imaging & Optics Facility (IOF) and the MiBa Machine Shop. Specifically; Robert Hauschild (IOF), sharing designs, insights and pioneering 3D printing activities at the Imaging and Optics Facility; Bernhard Hochreiter (IOF), for support and testing of anoxic chamber. We also thank Ana Rita Carvalho Faria and Oliver Biehlmaier (Biozentrum University of Basel, Imaging Core Facility) for sharing the design of the adopted power meter.\r\nOpen Access funding provided by Institute of Science and Technology Austria.","PlanS_conform":"1","title":"3D printing in core facilities – Low pain, high gain","volume":302,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"M-Shop"}],"month":"06","date_updated":"2026-07-27T14:02:46Z","quality_controlled":"1","external_id":{"pmid":["42104760"]},"das_tickbox":"0","publisher":"Wiley","scopus_import":"1","citation":{"ieee":"M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, and G. Krens, “3D printing in core facilities – Low pain, high gain,” <i>Journal of Microscopy</i>, vol. 302, no. 3. Wiley, pp. 382–395, 2026.","ama":"Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 3D printing in core facilities – Low pain, high gain. <i>Journal of Microscopy</i>. 2026;302(3):382-395. doi:<a href=\"https://doi.org/10.1111/jmi.70106\">10.1111/jmi.70106</a>","apa":"Goudarzi, M., Schuster, M., Milberger, A., Gunkel, M., Terjung, S., &#38; Krens, G. (2026). 3D printing in core facilities – Low pain, high gain. <i>Journal of Microscopy</i>. Wiley. <a href=\"https://doi.org/10.1111/jmi.70106\">https://doi.org/10.1111/jmi.70106</a>","chicago":"Goudarzi, Mohammad, Maximilian Schuster, Arthur Milberger, Manuel Gunkel, Stefan Terjung, and Gabriel Krens. “3D Printing in Core Facilities – Low Pain, High Gain.” <i>Journal of Microscopy</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/jmi.70106\">https://doi.org/10.1111/jmi.70106</a>.","mla":"Goudarzi, Mohammad, et al. “3D Printing in Core Facilities – Low Pain, High Gain.” <i>Journal of Microscopy</i>, vol. 302, no. 3, Wiley, 2026, pp. 382–95, doi:<a href=\"https://doi.org/10.1111/jmi.70106\">10.1111/jmi.70106</a>.","short":"M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, G. Krens, Journal of Microscopy 302 (2026) 382–395.","ista":"Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 2026. 3D printing in core facilities – Low pain, high gain. Journal of Microscopy. 302(3), 382–395."},"abstract":[{"lang":"eng","text":"Three-dimensional (3D) printing has rapidly developed from a niche hobbyist activity into a widely accessible and indispensable technology across multiple scientific disciplines. Within microscopy, optical engineering laboratories and imaging core facilities, 3D printing enables creating customised solutions for sample holders, optical components and everyday laboratory tools that traditionally required specialised machining. By providing rapid prototyping, low-cost production and reproducibility, 3D printing facilitates innovation and efficiency in facility operations. This article provides a perspective on the possibilities, challenges, and practical aspects of implementing 3D printing within microscopy core facilities. Instead of providing technical review about 3D printing, we focus on service organisation, user engagement, resource management and community-driven repositories for design dissemination. Our aim is to share insights with those considering the implementation of 3D printing as a service for developing add-on components to ease the operation of different aspects of the machine-park driven services and those who are managing advanced instrumentation within research groups."}],"oa_version":"Published Version","status":"public","type":"journal_article","publication_identifier":{"eissn":["1365-2818"],"issn":["0022-2720"]},"supplementarymaterial":"no","OA_type":"hybrid","article_type":"original","date_created":"2026-05-17T22:02:11Z","year":"2026","intvolume":"       302","oa":1,"publication_status":"published","file":[{"date_created":"2026-07-27T14:01:34Z","success":1,"file_id":"22593","creator":"dernst","checksum":"06dfad92b1465ed614a1201b4129960a","file_name":"2026_JourMicroscopy_Goudarzi.pdf","access_level":"open_access","file_size":4625767,"date_updated":"2026-07-27T14:01:34Z","relation":"main_file","content_type":"application/pdf"}],"doi":"10.1111/jmi.70106","ddc":["600"],"language":[{"iso":"eng"}],"author":[{"first_name":"Mohammad","last_name":"Goudarzi","id":"3384113A-F248-11E8-B48F-1D18A9856A87","full_name":"Goudarzi, Mohammad"},{"first_name":"Maximilian","last_name":"Schuster","full_name":"Schuster, Maximilian","id":"37e65def-d415-11eb-ae59-a7b67be103db"},{"full_name":"Milberger, Arthur","first_name":"Arthur","last_name":"Milberger"},{"full_name":"Gunkel, Manuel","first_name":"Manuel","last_name":"Gunkel"},{"full_name":"Terjung, Stefan","first_name":"Stefan","last_name":"Terjung"},{"orcid":"0000-0003-4761-5996","first_name":"Gabriel","last_name":"Krens","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87"}],"file_date_updated":"2026-07-27T14:01:34Z","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"has_accepted_license":"1","page":"382-395","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21883","department":[{"_id":"Bio"}],"issue":"3","publication":"Journal of Microscopy","corr_author":"1","OA_place":"publisher","day":"01","researchdata_availability":"no"},{"date_updated":"2026-07-27T14:13:17Z","quality_controlled":"1","external_id":{"pmid":["42185081"]},"month":"07","volume":54,"article_processing_charge":"No","date_published":"2026-07-01T00:00:00Z","title":"Emerging bioethical conflicts: One Health and animal experimentation","OA_type":"closed access","status":"public","type":"journal_article","publication_identifier":{"eissn":["2632-3559"],"issn":["0261-1929"]},"scopus_import":"1","citation":{"chicago":"Ulman, Yesim Isil, Nikos Kostomitsopoulos, Samuel Camenzind, Maria Kitsara, Ilja Richard Pavone, and Sophie Schober. “Emerging Bioethical Conflicts: One Health and Animal Experimentation.” <i>Alternatives to Laboratory Animals</i>. SAGE Publications, 2026. <a href=\"https://doi.org/10.1177/02611929261453330\">https://doi.org/10.1177/02611929261453330</a>.","apa":"Ulman, Y. I., Kostomitsopoulos, N., Camenzind, S., Kitsara, M., Pavone, I. R., &#38; Schober, S. (2026). Emerging bioethical conflicts: One Health and animal experimentation. <i>Alternatives to Laboratory Animals</i>. SAGE Publications. <a href=\"https://doi.org/10.1177/02611929261453330\">https://doi.org/10.1177/02611929261453330</a>","short":"Y.I. Ulman, N. Kostomitsopoulos, S. Camenzind, M. Kitsara, I.R. Pavone, S. Schober, Alternatives to Laboratory Animals 54 (2026) 226–235.","ista":"Ulman YI, Kostomitsopoulos N, Camenzind S, Kitsara M, Pavone IR, Schober S. 2026. Emerging bioethical conflicts: One Health and animal experimentation. Alternatives to Laboratory Animals. 54(4), 226–235.","mla":"Ulman, Yesim Isil, et al. “Emerging Bioethical Conflicts: One Health and Animal Experimentation.” <i>Alternatives to Laboratory Animals</i>, vol. 54, no. 4, SAGE Publications, 2026, pp. 226–35, doi:<a href=\"https://doi.org/10.1177/02611929261453330\">10.1177/02611929261453330</a>.","ieee":"Y. I. Ulman, N. Kostomitsopoulos, S. Camenzind, M. Kitsara, I. R. Pavone, and S. Schober, “Emerging bioethical conflicts: One Health and animal experimentation,” <i>Alternatives to Laboratory Animals</i>, vol. 54, no. 4. SAGE Publications, pp. 226–235, 2026.","ama":"Ulman YI, Kostomitsopoulos N, Camenzind S, Kitsara M, Pavone IR, Schober S. Emerging bioethical conflicts: One Health and animal experimentation. <i>Alternatives to Laboratory Animals</i>. 2026;54(4):226-235. doi:<a href=\"https://doi.org/10.1177/02611929261453330\">10.1177/02611929261453330</a>"},"abstract":[{"lang":"eng","text":"One Health initiatives are modern paradigms for research and health care practices in various fields. Concrete definitions of the One Health framework, however, remain heterogeneous, leading to conceptual problems and uncertainties in the application of the framework. This article discusses several approaches to the One Health concept, and their associated consequences, with special focus on animal experimentation. The first issue addressed is how One Health should be defined, as well as what (and who) should be considered within a One Health approach. In order to shed further light on this, we explore the history of animals in biomedical science, highlighting historical milestones in the use of animal models, as well as the development and current state of ethical considerations in the field of animal experimentation. The second issue comes with the inclusion of animal experimentation per se as part of the One Health concept. Therefore, particular attention is paid to bioethical principles and the resulting problems that can arise when applying them to the One Health concept. Arguments such as the idea of inequality between humans and non-human animals, and the premise that all actions are done for the benefit of humans, are raised and then used to explore the question of whether the One Health concept is compatible with existing bioethical principles. Based on the bioethical principles of protecting the environment, the biodiversity and biosphere, this paper seeks an inclusive perspective of the One Health concept. Successful solutions will be based on this concept, which embraces all living beings. The authors conclude that a multispecies ethics approach could help create a more ethical ecosystem that is aligned with the wellbeing of all life on a shared planet."}],"oa_version":"None","das_tickbox":"1","publisher":"SAGE Publications","language":[{"iso":"eng"}],"author":[{"full_name":"Ulman, Yesim Isil","first_name":"Yesim Isil","last_name":"Ulman"},{"first_name":"Nikos","last_name":"Kostomitsopoulos","full_name":"Kostomitsopoulos, Nikos"},{"full_name":"Camenzind, Samuel","last_name":"Camenzind","first_name":"Samuel"},{"last_name":"Kitsara","first_name":"Maria","full_name":"Kitsara, Maria"},{"last_name":"Pavone","first_name":"Ilja Richard","full_name":"Pavone, Ilja Richard"},{"last_name":"Schober","first_name":"Sophie","full_name":"Schober, Sophie","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8"}],"intvolume":"        54","publication_status":"published","doi":"10.1177/02611929261453330","year":"2026","article_type":"original","date_created":"2026-06-07T22:01:36Z","day":"01","publication":"Alternatives to Laboratory Animals","corr_author":"1","pmid":1,"_id":"21950","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"PreCl"}],"issue":"4","page":"226-235"}]