[{"publication_status":"submitted","oa":1,"has_accepted_license":"1","acknowledgement":"We thank Kay-Uwe Wagner (Wayne State University) for generously sharing Jak1/2–flox mouse lines; A.\r\nSommer (VBCF GmbH, NGS Unit) for technical support; N. Kim, V. Mick, S. Schnabl, S. Gobeil, and L.\r\nAndersen for technical assistance; all members of the Hippenmeyer lab for discussion and B. Novitch for\r\ncomments on earlier versions of the manuscript. This research was supported by the Scientific Service Units\r\n(SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). O.A.M received support from the Austrian Academy of Sciences\r\nÖAW (DOC 186584), and N.A. from FWF Elise Richter Program (Grant V1041T). This work was also\r\nsupported by IST Austria institutional funds; FWF SFB F78 (Neuro Stem Modulation) to S.H., and the\r\nEuropean Research Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogramme (grant agreement No 725780 LinPro) to S.H.","publication":"bioRxiv","month":"05","date_updated":"2026-06-16T08:57:20Z","type":"preprint","abstract":[{"text":"The cerebral cortex consists of immense numbers of neuronal and glial cell-types derived from radial glial progenitor (RGP) cells. How RGPs generate appropriate quantities of distinct cortical cell-types to safeguard a brain of correct size, is not well understood. However, genetic aberration in human, including mutations in PTEN, lead to cortical malformation such as macrocephaly, albeit with unknown etiology. Here we utilized Mosaic Analysis with Double Markers (MADM)-based clonal analysis and single cell phenotyping to decipher the role of Pten in neurogenic and gliogenic RGP lineage progression during cortical ontogeny. While neurogenic RGP lineage progression and projection neuron production was moderately altered in the absence of Pten, cortical astrocyte production was drastically increased. Through genetic epistasis experiments we show that the loss of Pten uncouples astrocyte generation from essential growth factor signaling hubs, funneling into MAPK. Collectively, our results suggest that Pten regulates RGP lineage progression with distinct sequential functions in cortical projection neurogenesis and astrocyte production to ensure the emergence of a correctly-sized cerebral cortex.","lang":"eng"}],"department":[{"_id":"SiHi"},{"_id":"PreCl"},{"_id":"GradSch"}],"ddc":["570"],"OA_type":"green","status":"public","date_published":"2026-05-05T00:00:00Z","title":"Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production","citation":{"short":"O. Miranda, X. Contreras, F. Pauler, A. Davaatseren, N. Amberg, C. Streicher, A. Villalba Requena, A.-M. Heger, C. Marie, B.A. Hassan, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.).","mla":"Miranda, Osvaldo, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” BioRxiv, doi:10.64898/2026.05.01.722191.","chicago":"Miranda, Osvaldo, Ximena Contreras, Florian Pauler, Amarbayasgalan Davaatseren, Nicole Amberg, Carmen Streicher, Ana Villalba Requena, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” BioRxiv, n.d. https://doi.org/10.64898/2026.05.01.722191.","ieee":"O. Miranda et al., “Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production,” bioRxiv. .","apa":"Miranda, O., Contreras, X., Pauler, F., Davaatseren, A., Amberg, N., Streicher, C., … Hippenmeyer, S. (n.d.). Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv. https://doi.org/10.64898/2026.05.01.722191","ista":"Miranda O, Contreras X, Pauler F, Davaatseren A, Amberg N, Streicher C, Villalba Requena A, Heger A-M, Marie C, Hassan BA, Rülicke T, Hippenmeyer S. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv, 10.64898/2026.05.01.722191.","ama":"Miranda O, Contreras X, Pauler F, et al. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv. doi:10.64898/2026.05.01.722191"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Osvaldo","full_name":"Miranda, Osvaldo","orcid":"0000-0001-6618-6889","last_name":"Miranda","id":"862A3C56-A8BF-11E9-B4FA-D9E3E5697425"},{"full_name":"Contreras, Ximena","first_name":"Ximena","id":"475990FE-F248-11E8-B48F-1D18A9856A87","last_name":"Contreras"},{"full_name":"Pauler, Florian","first_name":"Florian","last_name":"Pauler","id":"48EA0138-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7462-0048"},{"last_name":"Davaatseren","id":"70ADC922-B424-11E9-99E3-BA18E6697425","full_name":"Davaatseren, Amarbayasgalan","first_name":"Amarbayasgalan"},{"full_name":"Amberg, Nicole","first_name":"Nicole","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","last_name":"Amberg","orcid":"0000-0002-3183-8207"},{"id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher","first_name":"Carmen","full_name":"Streicher, Carmen"},{"full_name":"Villalba Requena, Ana","first_name":"Ana","last_name":"Villalba Requena","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","orcid":"0000-0002-5615-5277"},{"first_name":"Anna-Magdalena","full_name":"Heger, Anna-Magdalena","last_name":"Heger","id":"4B76FFD2-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Marie","first_name":"Corentine","full_name":"Marie, Corentine"},{"first_name":"Bassem A.","full_name":"Hassan, Bassem A.","last_name":"Hassan"},{"last_name":"Rülicke","full_name":"Rülicke, Thomas","first_name":"Thomas"},{"first_name":"Simon","full_name":"Hippenmeyer, Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061"}],"doi":"10.64898/2026.05.01.722191","OA_place":"repository","oa_version":"Preprint","ec_funded":1,"project":[{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression","_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","grant_number":"F7805"},{"call_identifier":"H2020","name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","grant_number":"725780","_id":"260018B0-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"No","date_created":"2026-06-09T08:08:53Z","year":"2026","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"main_file_link":[{"url":"https://doi.org/10.64898/2026.05.01.722191","open_access":"1"}],"corr_author":"1","tmp":{"image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)"},"language":[{"iso":"eng"}],"_id":"21963","day":"05"},{"scopus_import":"1","publication_identifier":{"eissn":["2041-1723"]},"ec_funded":1,"das_tickbox":"1","article_processing_charge":"Yes","project":[{"call_identifier":"H2020","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","grant_number":"692692","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"},{"name":"Synaptic mechanisms of engram storage and retrieval in CA3 hippocampal microcircuits","_id":"e62b56fe-ab3c-11f0-94c7-d181dd352b3b","grant_number":"101199096"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020"},{"_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","grant_number":"101026635","name":"Synaptic computations of the hippocampal CA3 circuitry","call_identifier":"H2020"},{"name":"Mechanisms of GABA release in hippocampal circuits","grant_number":"P36232","_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5"},{"_id":"8d9195e9-16d5-11f0-9cad-d075be887a1e","grant_number":"PAT 4178023","name":"Synaptic networks of human brain"},{"name":"Reglas de Conectividad funcional en el hipocampo","_id":"26366136-B435-11E9-9278-68D0E5697425"}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"M-Shop"},{"_id":"ScienComp"}],"year":"2026","date_created":"2026-06-30T13:05:52Z","day":"23","intvolume":" 17","_id":"22229","language":[{"iso":"eng"}],"file":[{"date_created":"2026-07-01T06:46:06Z","file_size":18304997,"relation":"main_file","checksum":"d0b0093493926985b4c268662ff4d556","content_type":"application/pdf","access_level":"open_access","file_name":"2026_NatureComm_VargasBarroso.pdf","creator":"dernst","success":1,"file_id":"22231","date_updated":"2026-07-01T06:46:06Z"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"corr_author":"1","related_material":{"record":[{"id":"21442","status":"public","relation":"research_data"}]},"has_accepted_license":"1","acknowledgement":"We thank Jose Guzman, Simon Hippenmeyer, and Tim Vogels for critically reading the manuscript, Jozsef Csicsvari for useful discussions, Florian Marr for technical assistance, and Eleftheria Kralli-Beller for manuscript editing. This research was supported by the Scientific Services Units (SSUs) of ISTA: the preclinical facility (PCF) provided housing and breeding of the animals, the imaging and optics facility (IOF) offered technical training and state of the art equipment, the Miba machine shop contributed to the construction and maintenance of multicellular recording setups, and the scientific computing unit helped with the large-scale simulations. The project received funding from the European Union’s Horizon 2020 research and innovation programme (ERC Advanced Grants No 692692 GIANTSYN and 101199096 CA3-SYNGRAM to P.J.; Marie Skłodowska-Curie Grant 754411 to V.V.B.; Marie Skłodowska-Curie Grant 101026635 to J.F.W.), the Fond zur Förderung der Wissenschaftlichen Forschung (P 36232-B, PAT4178023, and 10.55776/CoE16 to P.J.), and the Nomis Foundation (fellowship to A.N.-O.). V.V.B. received funding from a CONACyT fellowship (289638).","PlanS_conform":"1","supplementarymaterial":"yes","file_date_updated":"2026-07-01T06:46:06Z","oa":1,"publication_status":"published","date_updated":"2026-07-01T06:47:49Z","volume":17,"month":"06","quality_controlled":"1","DOAJ_listed":"1","publication":"Nature Communications","article_type":"original","OA_type":"gold","dataavailabilitystatement":"Source data are provided with this paper. Additional original data are available from the corresponding author upon request. Code is available from https://doi.org/10.15479/AT-ISTA-21442 under the link https://research-explorer.ista.ac.at/download/21442/21443/ca3simu-vargas2026v1.tar.gz","department":[{"_id":"PeJo"},{"_id":"ScienComp"}],"ddc":["570"],"abstract":[{"lang":"eng","text":"Hippocampal CA3 pyramidal neurons (PNs) form the largest autoassociative network in the mammalian brain. Whether CA3–CA3 recurrent connectivity is genetically preconfigured or environmentally shaped during ongoing memory storage is currently unknown. To address this question, we performed multicellular patch-clamp-based circuit mapping of up to eight CA3 PNs in the mouse hippocampus at multiple postnatal time points (P7–8, P18–25, and P45–50). Here, we show that the hippocampal CA3 network undergoes a developmental transformation from local, dense, and random connectivity to a distributed, sparse, and structured configuration. Thus, sparse and structured connectivity may emerge via experience-dependent mechanisms. In parallel, the strength of single synapses is downregulated; single synaptic events are sufficient to trigger postsynaptic spiking early in development, whereas spatial summation of several inputs is required at later time points. Biologically inspired models of memory storage by Hebbian synaptic plasticity and retrieval via pattern completion suggest that developmental changes improve specific aspects of memory storage and retrieval. Our results imply a developmental transformation of the neuronal code and the memory functions in the hippocampal CA3 network."}],"type":"journal_article","oa_version":"Published Version","OA_place":"publisher","doi":"10.1038/s41467-026-71914-x","pmid":1,"author":[{"id":"2F55A9DE-F248-11E8-B48F-1D18A9856A87","last_name":"Vargas Barroso","full_name":"Vargas Barroso, Victor M","first_name":"Victor M"},{"first_name":"Jake","full_name":"Watson, Jake","last_name":"Watson","id":"63836096-4690-11EA-BD4E-32803DDC885E","orcid":"0000-0002-8698-3823"},{"full_name":"Navas Olivé, Andrea C","first_name":"Andrea C","orcid":"0000-0002-9280-8597","last_name":"Navas Olivé","id":"739d26c9-52e8-11ee-8d72-f14d3893b4ce"},{"orcid":"0000-0002-5621-8100","last_name":"Schlögl","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","first_name":"Alois","full_name":"Schlögl, Alois"},{"first_name":"Peter M","full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"short":"V.M. Vargas Barroso, J. Watson, A.C. Navas Olivé, A. Schlögl, P.M. Jonas, Nature Communications 17 (2026).","mla":"Vargas Barroso, Victor M., et al. “Developmental Emergence of Sparse and Structured Synaptic Connectivity in the Hippocampal CA3 Memory Circuit.” Nature Communications, vol. 17, 5540, Springer Nature, 2026, doi:10.1038/s41467-026-71914-x.","chicago":"Vargas Barroso, Victor M, Jake Watson, Andrea C Navas Olivé, Alois Schlögl, and Peter M Jonas. “Developmental Emergence of Sparse and Structured Synaptic Connectivity in the Hippocampal CA3 Memory Circuit.” Nature Communications. Springer Nature, 2026. https://doi.org/10.1038/s41467-026-71914-x.","ieee":"V. M. Vargas Barroso, J. Watson, A. C. Navas Olivé, A. Schlögl, and P. M. Jonas, “Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit,” Nature Communications, vol. 17. Springer Nature, 2026.","ista":"Vargas Barroso VM, Watson J, Navas Olivé AC, Schlögl A, Jonas PM. 2026. Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. Nature Communications. 17, 5540.","apa":"Vargas Barroso, V. M., Watson, J., Navas Olivé, A. C., Schlögl, A., & Jonas, P. M. (2026). Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-026-71914-x","ama":"Vargas Barroso VM, Watson J, Navas Olivé AC, Schlögl A, Jonas PM. Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. Nature Communications. 2026;17. doi:10.1038/s41467-026-71914-x"},"publisher":"Springer Nature","article_number":"5540","title":"Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit","date_published":"2026-06-23T00:00:00Z","external_id":{"pmid":["42014695"]},"researchdata_availability":"yes","status":"public"},{"type":"software","year":"2026","date_created":"2026-03-12T08:20:46Z","department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"doi":"10.15479/AT-ISTA-21442","_id":"21442","keyword":["hypocampus","ca3 simulations","modelling"],"day":"12","user_id":"68b8ca59-c5b3-11ee-8790-cd641c68093d","corr_author":"1","related_material":{"record":[{"relation":"used_in_publication","status":"public","id":"22229"}]},"publisher":"Institute of Science and Technology Austria","citation":{"ieee":"A. Schlögl, “CA3Simu v1.06 (vargas2026v1).” Institute of Science and Technology Austria, 2026.","ista":"Schlögl A. 2026. CA3Simu v1.06 (vargas2026v1), Institute of Science and Technology Austria, 10.15479/AT-ISTA-21442.","apa":"Schlögl, A. (2026). CA3Simu v1.06 (vargas2026v1). Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21442","ama":"Schlögl A. CA3Simu v1.06 (vargas2026v1). 2026. doi:10.15479/AT-ISTA-21442","mla":"Schlögl, Alois. CA3Simu v1.06 (Vargas2026v1). Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-21442.","short":"A. Schlögl, (2026).","chicago":"Schlögl, Alois. “CA3Simu v1.06 (Vargas2026v1).” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21442."},"tmp":{"short":"GPL 3.0","legal_code_url":"https://www.gnu.org/licenses/gpl-3.0.en.html","name":"GNU General Public License 3.0"},"author":[{"full_name":"Schlögl, Alois","first_name":"Alois","orcid":"0000-0002-5621-8100","last_name":"Schlögl","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87"}],"file":[{"relation":"main_file","date_created":"2026-03-12T08:19:14Z","file_size":160410,"content_type":"application/gzip","checksum":"441c8827717dcda05f91c127d15cf1e9","access_level":"open_access","file_name":"ca3simu-vargas2026v1.tar.gz","date_updated":"2026-03-12T08:19:14Z","success":1,"file_id":"21443","creator":"schloegl"},{"access_level":"open_access","checksum":"3c0092076228a15c0a7ae703192d43ea","content_type":"text/markdown","relation":"main_file","file_size":10923,"date_created":"2026-03-12T10:24:45Z","date_updated":"2026-03-12T10:24:45Z","success":1,"creator":"schloegl","file_id":"21445","file_name":"README.md"}],"title":"CA3Simu v1.06 (vargas2026v1)","date_published":"2026-03-12T00:00:00Z","status":"public","has_accepted_license":"1","oa":1,"file_date_updated":"2026-03-12T10:24:45Z","ec_funded":1,"date_updated":"2026-07-01T06:47:49Z","month":"03","project":[{"name":"Synaptic mechanisms of engram storage and retrieval in CA3 hippocampal microcircuits","_id":"e62b56fe-ab3c-11f0-94c7-d181dd352b3b","grant_number":"101199096"},{"name":"Mechanisms of GABA release in hippocampal circuits","_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","grant_number":"P36232"},{"name":"Synaptic networks of human brain","_id":"8d9195e9-16d5-11f0-9cad-d075be887a1e","grant_number":"PAT 4178023"},{"grant_number":"692692","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse"}]},{"file":[{"file_name":"2026_MicrobialBiotechnology_Kralova.pdf","file_id":"22271","success":1,"creator":"dernst","date_updated":"2026-07-13T06:57:19Z","date_created":"2026-07-13T06:57:19Z","file_size":2497486,"relation":"main_file","content_type":"application/pdf","checksum":"4f735714644f1049b22b014225843d8d","access_level":"open_access"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"corr_author":"1","day":"01","keyword":["Actinokineospora","Antarctica","antimicrobial discovery","biosynthetic gene cluster","genome mining","microbial competition","nonribosomalpeptide synthetase","siderophores"],"language":[{"iso":"eng"}],"_id":"22254","intvolume":" 19","date_created":"2026-07-08T09:19:43Z","year":"2026","article_processing_charge":"Yes","das_tickbox":"1","publication_identifier":{"eissn":["1751-7915"]},"issue":"6","scopus_import":"1","status":"public","title":"Kineochelins - A new group of siderophores from an antarctic bacterium","date_published":"2026-06-01T00:00:00Z","article_number":"e70386","external_id":{"biorxivid":["10.64898/2026.02.23.707395"],"pmid":["42210522"]},"author":[{"last_name":"Kralova","first_name":"Stanislava","full_name":"Kralova, Stanislava"},{"last_name":"Spacek","first_name":"Peter","full_name":"Spacek, Peter"},{"last_name":"Gafriller","first_name":"Johannes","full_name":"Gafriller, Johannes"},{"last_name":"Bezdicek","full_name":"Bezdicek, Matej","first_name":"Matej"},{"first_name":"Viktoria","full_name":"Medvedcova, Viktoria","last_name":"Medvedcova"},{"last_name":"Séneca","first_name":"Joana","full_name":"Séneca, Joana"},{"full_name":"Osvatic, Jay","first_name":"Jay","last_name":"Osvatic"},{"first_name":"Ulrike","full_name":"Grienke, Ulrike","last_name":"Grienke"},{"last_name":"Rattei","first_name":"Thomas","full_name":"Rattei, Thomas"},{"full_name":"Sekurova, Olga N.","first_name":"Olga N.","last_name":"Sekurova"},{"last_name":"Zotchev","first_name":"Sergey B.","full_name":"Zotchev, Sergey B."},{"first_name":"Martin","full_name":"Zehl, Martin","id":"8e016d5b-5d77-11f0-86d2-96cdb3922a55","last_name":"Zehl","orcid":"0000-0001-9685-0373"},{"first_name":"Alexander","full_name":"Loy, Alexander","last_name":"Loy"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ieee":"S. Kralova et al., “Kineochelins - A new group of siderophores from an antarctic bacterium,” Microbial Biotechnology, vol. 19, no. 6. Wiley, 2026.","ista":"Kralova S, Spacek P, Gafriller J, Bezdicek M, Medvedcova V, Séneca J, Osvatic J, Grienke U, Rattei T, Sekurova ON, Zotchev SB, Zehl M, Loy A. 2026. Kineochelins - A new group of siderophores from an antarctic bacterium. Microbial Biotechnology. 19(6), e70386.","apa":"Kralova, S., Spacek, P., Gafriller, J., Bezdicek, M., Medvedcova, V., Séneca, J., … Loy, A. (2026). Kineochelins - A new group of siderophores from an antarctic bacterium. Microbial Biotechnology. Wiley. https://doi.org/10.1111/1751-7915.70386","ama":"Kralova S, Spacek P, Gafriller J, et al. Kineochelins - A new group of siderophores from an antarctic bacterium. Microbial Biotechnology. 2026;19(6). doi:10.1111/1751-7915.70386","short":"S. Kralova, P. Spacek, J. Gafriller, M. Bezdicek, V. Medvedcova, J. Séneca, J. Osvatic, U. Grienke, T. Rattei, O.N. Sekurova, S.B. Zotchev, M. Zehl, A. Loy, Microbial Biotechnology 19 (2026).","mla":"Kralova, Stanislava, et al. “Kineochelins - A New Group of Siderophores from an Antarctic Bacterium.” Microbial Biotechnology, vol. 19, no. 6, e70386, Wiley, 2026, doi:10.1111/1751-7915.70386.","chicago":"Kralova, Stanislava, Peter Spacek, Johannes Gafriller, Matej Bezdicek, Viktoria Medvedcova, Joana Séneca, Jay Osvatic, et al. “Kineochelins - A New Group of Siderophores from an Antarctic Bacterium.” Microbial Biotechnology. Wiley, 2026. https://doi.org/10.1111/1751-7915.70386."},"publisher":"Wiley","oa_version":"Published Version","biorxivid":1,"OA_place":"publisher","doi":"10.1111/1751-7915.70386","pmid":1,"ddc":["570"],"department":[{"_id":"MassSpec"}],"abstract":[{"lang":"eng","text":"The global rise of antimicrobial resistance has intensified the search for new microbial metabolites from underexplored environments and taxonomic groups. Extreme and geographically isolated habitats such as Antarctic terrestrial ecosystems represent promising reservoirs of biosynthetic diversity, particularly among rare and difficult-to-cultivate actinomycetes that may produce chemically diverse metabolites with potential biotechnological applications. Here, we report the characterization of kineochelins, a previously undescribed group of siderophores produced by the Antarctic isolate Actinokineospora sp. UV203, representing a difficult-to-cultivate actinomycete lineage. Structural elucidation revealed a set of closely related congeners with a mixed-ligand architecture consistent with metal-chelating activity. Genome mining combined with transcriptomic analysis identified a dedicated nonribosomal peptide synthetase-encoding biosynthetic gene cluster responsible for kineochelin production. Comparative genomic analyses indicated that, although kineochelin biosynthetic genes share limited similarity with known mixed-ligand siderophores, their gene content and organization differ substantially, suggesting a distinct biosynthetic lineage. Functional characterization of the culture supernatant and an enriched pre-purified kineochelin fraction demonstrated strong and selective iron chelation, with high affinity for ferric and ferrous iron. Crude culture extracts inhibited the growth of bacterial strains isolated from the same Antarctic environment, indicating that kineochelins may contribute to iron-mediated microbial competition. In addition, kineochelin-enriched pre-purified fractions showed moderate selective inhibitory activity against the opportunistic yeast pathogen Nakaseomyces glabratus and a clinical isolate of Saccharomyces cerevisiae associated with invasive infection. These findings expand the chemical and biosynthetic diversity known within the genus Actinokineospora and demonstrate that Antarctic rare actinomycetes represent valuable sources of previously unexplored natural products. The discovery of kineochelins highlights the potential of genome-guided exploration of polar microorganisms for identifying bioactive metabolites with relevance for antimicrobial discovery and biotechnology."}],"type":"journal_article","dataavailabilitystatement":"The genome sequence and transcriptomic data of strain Actinokineospora sp. UV203 are available on NCBI (BioProject accession number PRJNA1331526). The nearly full-length 16S rRNA gene (1395 bp) of strain Actinokineospora sp. UV203 is available on NCBI (accession number PX090945). The NMR data of kineochelin E1 and A1 are deposited in the Natural Products Magnetic Resonance Database (NP-MRD) under accession numbers NP0352113 and NP0352114, respectively.","article_type":"original","OA_type":"gold","publication":"Microbial Biotechnology","quality_controlled":"1","month":"06","DOAJ_listed":"1","volume":19,"date_updated":"2026-07-13T06:59:08Z","publication_status":"published","supplementarymaterial":"yes","file_date_updated":"2026-07-13T06:57:19Z","oa":1,"acknowledgement":"This work was supported by the Czech Antarctic Research Programme 2025–2027 (VAN 2025) and the University of Vienna via the Research Platform Secondary Metabolomes of Bacterial Communities (MetaBac). S.K. has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 101020356 (DEFCOMANT, https://doi.org/10.3030/101020356) and MASH StG/CoG (MUNI/SC/1946/2024) by Masaryk University. T.R. and A.L. were funded in part by the Austrian Science Fund FWF [grant DOI https://doi.org/10.55776/COE7]. M.B. was funded by the Ministry of Health, Czech Republic—conceptual development of research organization (FNBr, 65269705). The Life Science Compute Cluster LiSC at the University of Vienna provided the high-performance computing infrastructure for this study. We thank Julia Ramesmayer and Sara Malinowski (Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna) for assistance during high molecular weight extraction and RNA extraction. The authors thank Anna Fabisikova and Michael Klemm-Abraham from the Mass Spectrometry Centre and the team of the NMR Centre (both core facilities of the Faculty of Chemistry, University of Vienna, and members of the Vienna Life Science Instruments) for assistance with data acquisition. We are thankful to Dr. Jaime Felipe Guerrero Garzón for helpful discussions on the use of a rrn operon promoter strategy. For open access purposes, the authors have applied for a CC BY public copyright licence to any author-accepted manuscript version arising from this submission. Dr. Martin Kello (Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia) and Dr. Michal Goga (Department of Plant Biology, Faculty of Science and Center for Interdisciplinary Biosciences, Technology and Innovation Park, Pavol Jozef Šafárik University in Košice, Košice, Slovakia), funded by VEGA 1/0498/23, are acknowledged for their assistance with the antiproliferative assays. This work was supported by Horizon 2020 Framework Programme, 101020356; Universität Wien, MetaBac; Ministry of Education, Youth and Sports, VAN 2025; Masarykova Univerzita, MUNI/SC/1946/2024; Austrian Science Fund, 10.55776/COE7; Ministerstvo Zdravotnictví České Republiky, FNBr, 65269705; Vedecká grantová agentúra Ministerstva školstva, výskumu, vývoja a mládeže Slovenskej republiky a Slovenskej akadémie vied, VEGA 1/0498/23.","has_accepted_license":"1"},{"article_processing_charge":"Yes","scopus_import":"1","publication_identifier":{"eissn":["2041-6539"],"issn":["2041-6520"]},"das_tickbox":"1","_id":"22289","language":[{"iso":"eng"}],"day":"09","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"date_created":"2026-07-13T09:41:36Z","year":"2026","date_updated":"2026-07-13T11:24:29Z","DOAJ_listed":"1","quality_controlled":"1","month":"06","publication":"Chemical Science","has_accepted_license":"1","acknowledgement":"We acknowledge financial support from the European Research Council (ERC) under the European Union's Horizon Europe Framework Programme (grant agreement no. ERC-2021-ADG-320892) and from the China Scholarship Council (CSC, predoctoral fellowship to J. W.). We thank L. Allmendinger for assistance with NMR measurements, P. Mayer for his assistance in solving the crystal structures of 1 and 1d, L. Bodero for assistance with automated solid-phase synthesis, M. Rogovoi for providing monomer precursors, and M. Loos for the purification and analysis of compounds 15a–19a. We thank M. Soler-Lopez (ID23-1, ESRF, Grenoble) and I. Bento (EMBL P13, Petra III, DESY, Hamburg) for assistance during data collection at the synchrotron beamlines.","supplementarymaterial":"yes","publication_status":"inpress","doi":"10.1039/d6sc00798h","oa_version":"Published Version","OA_place":"publisher","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Wu, Jiaojiao, Valentina Corvaglia, Tulika Chakrabortty, Pradeep K Mandal, and Ivan Huc. “Tailoring the Major Groove of DNA Mimic Foldamers.” Chemical Science. Royal Society of Chemistry, n.d. https://doi.org/10.1039/d6sc00798h.","short":"J. Wu, V. Corvaglia, T. Chakrabortty, P.K. Mandal, I. Huc, Chemical Science (n.d.).","mla":"Wu, Jiaojiao, et al. “Tailoring the Major Groove of DNA Mimic Foldamers.” Chemical Science, Royal Society of Chemistry, doi:10.1039/d6sc00798h.","ama":"Wu J, Corvaglia V, Chakrabortty T, Mandal PK, Huc I. Tailoring the major groove of DNA mimic foldamers. Chemical Science. doi:10.1039/d6sc00798h","ieee":"J. Wu, V. Corvaglia, T. Chakrabortty, P. K. Mandal, and I. Huc, “Tailoring the major groove of DNA mimic foldamers,” Chemical Science. Royal Society of Chemistry.","ista":"Wu J, Corvaglia V, Chakrabortty T, Mandal PK, Huc I. Tailoring the major groove of DNA mimic foldamers. Chemical Science.","apa":"Wu, J., Corvaglia, V., Chakrabortty, T., Mandal, P. K., & Huc, I. (n.d.). Tailoring the major groove of DNA mimic foldamers. Chemical Science. Royal Society of Chemistry. https://doi.org/10.1039/d6sc00798h"},"publisher":"Royal Society of Chemistry","author":[{"first_name":"Jiaojiao","full_name":"Wu, Jiaojiao","last_name":"Wu"},{"first_name":"Valentina","full_name":"Corvaglia, Valentina","last_name":"Corvaglia"},{"full_name":"Chakrabortty, Tulika","first_name":"Tulika","last_name":"Chakrabortty"},{"orcid":"0000-0001-5996-956X","last_name":"Mandal","id":"6a3def15-d4b4-11ef-9fa9-a24c1f545ec3","first_name":"Pradeep K","full_name":"Mandal, Pradeep K"},{"first_name":"Ivan","full_name":"Huc, Ivan","last_name":"Huc"}],"title":"Tailoring the major groove of DNA mimic foldamers","date_published":"2026-06-09T00:00:00Z","researchdata_availability":"yes","status":"public","OA_type":"gold","article_type":"original","dataavailabilitystatement":"CCDC 2514117, 2514118, 2286782 and 2478322 (compound 1, compound 1d, oligomer 5, and oligomer 6, respectively) contain the supplementary crystallographic data for this paper.54a–d \r\n\r\nThe supporting data have been provided as part of the supplementary information (SI). Supplementary information: SI figures, detailed experimental protocols, crystallographic studies, and characterisation of new compounds. See DOI: https://doi.org/10.1039/d6sc00798h.","abstract":[{"text":"Single-stranded, helically folded aromatic oligoamides bearing anionic phosphonate side chains have been shown to bind to some DNA-binding proteins better than DNA itself. However, these DNA mimic foldamers have until now mainly consisted of a single repeat motif, like a poly(dA:dT) DNA duplex, and contained limited sequence information. Here, we introduce new monomers designed to display different chemical functionalities in the major groove of the DNA mimics. Four new Fmoc-protected amino acid monomers have been synthesized and incorporated into oligomers. Sixteen foldamer sequences were prepared on solid phase. Their conformations in solution and in the solid state and their conformational dynamics were investigated using nuclear magnetic resonance, circular dichroism, molecular modeling, and X-ray crystallography. The results show that three of the four new monomers behaved as designed and that their introduction enhances the conformational dynamics of the DNA mimic foldamers. In a fourth case, conformational behavior proved to be more complex than expected. The modified sequences retained the ability to bind to the bacterial histone-like protein HU. These results showcase design strategies to manipulate large molecular biomimetics in which not only side chains but also main chain components are varied. The new monomers pave the way to complex DNA mimic foldamer sequences targeting proteins that recognize sequence-selective DNA-binding proteins such as transcription factors or restriction enzymes.","lang":"eng"}],"type":"journal_article","department":[{"_id":"LifeSc"}],"ddc":["540"]},{"article_processing_charge":"Yes (via OA deal)","project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"publication_identifier":{"issn":["1672-9072"],"eissn":["1744-7909"]},"scopus_import":"1","das_tickbox":"0","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"corr_author":"1","day":"10","_id":"22301","language":[{"iso":"eng"}],"main_file_link":[{"url":"https://doi.org/10.1111/jipb.70309","open_access":"1"}],"date_created":"2026-07-13T10:44:55Z","year":"2026","acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"date_updated":"2026-07-13T14:26:31Z","publication":"Journal of Integrative Plant Biology","month":"06","quality_controlled":"1","PlanS_conform":"1","acknowledgement":"Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria.","has_accepted_license":"1","publication_status":"epub_ahead","supplementarymaterial":"yes","oa":1,"author":[{"first_name":"Adrijana","full_name":"Smoljan, Adrijana","last_name":"Smoljan","id":"cced8a85-223e-11ed-af04-b0596c55053b"},{"full_name":"Koutnik‐Abele, Sarah","first_name":"Sarah","last_name":"Koutnik‐Abele"},{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev","first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii"},{"last_name":"Klíma","first_name":"Petr","full_name":"Klíma, Petr"},{"last_name":"Bírošíková","full_name":"Bírošíková, Anita","first_name":"Anita"},{"id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","last_name":"Zhang","orcid":"0000-0003-2627-6956","full_name":"Zhang, Yuzhou","first_name":"Yuzhou"},{"full_name":"Merrin, Jack","first_name":"Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin"},{"last_name":"Schuster","id":"37e65def-d415-11eb-ae59-a7b67be103db","full_name":"Schuster, Maximilian","first_name":"Maximilian"},{"first_name":"Katarina","full_name":"Kurtović, Katarina","last_name":"Kurtović"},{"last_name":"Hammes","first_name":"Ulrich Z.","full_name":"Hammes, Ulrich Z."},{"first_name":"Jan","full_name":"Petrášek, Jan","last_name":"Petrášek"},{"first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"citation":{"chicago":"Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology. Wiley, 2026. https://doi.org/10.1111/jipb.70309.","short":"A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026).","mla":"Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology, jipb. 70309, Wiley, 2026, doi:10.1111/jipb.70309.","ama":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology. 2026. doi:10.1111/jipb.70309","ieee":"A. Smoljan et al., “Auxin response and PIN‐mediated transport in chlorophyte algae,” Journal of Integrative Plant Biology. Wiley, 2026.","apa":"Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology. Wiley. https://doi.org/10.1111/jipb.70309","ista":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Wiley","oa_version":"Published Version","OA_place":"publisher","pmid":1,"doi":"10.1111/jipb.70309","status":"public","researchdata_availability":"no","article_number":"jipb.70309","title":"Auxin response and PIN‐mediated transport in chlorophyte algae","date_published":"2026-06-10T00:00:00Z","external_id":{"pmid":["42271607"]},"article_type":"original","OA_type":"hybrid","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"NanoFab"},{"_id":"Bio"}],"ddc":["580"],"abstract":[{"lang":"eng","text":"Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage."}],"type":"journal_article"},{"article_type":"original","page":"356-371.e12","OA_type":"hybrid","dataavailabilitystatement":"This study did not generate new unique reagents. Data are available upon request.\r\n•The custom-made codes used in this study are available at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"ddc":["570"],"abstract":[{"text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo.","lang":"eng"}],"type":"journal_article","OA_place":"publisher","oa_version":"Published Version","pmid":1,"doi":"10.1016/j.devcel.2025.10.006","author":[{"first_name":"Iván","full_name":"Company-Garrido, Iván","last_name":"Company-Garrido"},{"last_name":"Zurita Carpio","first_name":"Alberto","full_name":"Zurita Carpio, Alberto"},{"last_name":"Colomer-Rosell","full_name":"Colomer-Rosell, Mariona","first_name":"Mariona"},{"first_name":"Bernard","full_name":"Ciraulo, Bernard","last_name":"Ciraulo"},{"full_name":"Molkenbur, Ronja","first_name":"Ronja","last_name":"Molkenbur"},{"first_name":"Peter","full_name":"Lanzerstorfer, Peter","last_name":"Lanzerstorfer"},{"last_name":"Pezzano","full_name":"Pezzano, Fabio","first_name":"Fabio"},{"last_name":"Agazzi","first_name":"Costanza","full_name":"Agazzi, Costanza"},{"orcid":"0000-0001-9843-3522","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","first_name":"Robert"},{"last_name":"Jain","first_name":"Saumey","full_name":"Jain, Saumey"},{"last_name":"Jacques","first_name":"Jeroen M.","full_name":"Jacques, Jeroen M."},{"last_name":"Venturini","full_name":"Venturini, Valeria","first_name":"Valeria"},{"full_name":"Knapp, Christian","first_name":"Christian","last_name":"Knapp"},{"last_name":"Xie","first_name":"Yufei","full_name":"Xie, Yufei"},{"full_name":"Merrin, Jack","first_name":"Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Weghuber","first_name":"Julian","full_name":"Weghuber, Julian"},{"full_name":"Schaaf, Marcel","first_name":"Marcel","last_name":"Schaaf"},{"first_name":"Romain","full_name":"Quidant, Romain","last_name":"Quidant"},{"orcid":"0000-0001-6165-5738","last_name":"Kiermaier","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","full_name":"Kiermaier, Eva"},{"first_name":"Jaime","full_name":"Ortega Arroyo, Jaime","last_name":"Ortega Arroyo"},{"first_name":"Verena","full_name":"Ruprecht, Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","last_name":"Ruprecht","orcid":"0000-0003-4088-8633"},{"full_name":"Wieser, Stefan","first_name":"Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","last_name":"Wieser","orcid":"0000-0002-2670-2217"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Elsevier","citation":{"short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61 (2026) 356–371.e12.","mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” Developmental Cell, vol. 61, no. 2, Elsevier, 2026, p. 356–371.e12, doi:10.1016/j.devcel.2025.10.006.","chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” Developmental Cell. Elsevier, 2026. https://doi.org/10.1016/j.devcel.2025.10.006.","ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12.","apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.10.006","ieee":"I. Company-Garrido et al., “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” Developmental Cell, vol. 61, no. 2. Elsevier, p. 356–371.e12, 2026.","ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 2026;61(2):356-371.e12. doi:10.1016/j.devcel.2025.10.006"},"title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses","date_published":"2026-02-11T00:00:00Z","external_id":{"pmid":["41192429"]},"status":"public","researchdata_availability":"upon request","acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","has_accepted_license":"1","PlanS_conform":"1","supplementarymaterial":"yes","file_date_updated":"2026-07-23T06:26:25Z","oa":1,"publication_status":"published","date_updated":"2026-07-23T06:27:15Z","volume":61,"month":"02","quality_controlled":"1","publication":"Developmental Cell","acknowledged_ssus":[{"_id":"NanoFab"}],"year":"2026","date_created":"2025-12-28T23:01:27Z","keyword":["thermobiology","cell migration","thermo-adaptability of immune cells"],"day":"11","language":[{"iso":"eng"}],"_id":"20859","intvolume":" 61","file":[{"file_name":"2026_DevelopmentalCell_CompanyGarrido.pdf","file_id":"22388","creator":"dernst","success":1,"date_updated":"2026-07-23T06:26:25Z","date_created":"2026-07-23T06:26:25Z","file_size":12342817,"relation":"main_file","checksum":"52fd52d2d19a4514f8fcc1b40f420ca2","content_type":"application/pdf","access_level":"open_access"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"issue":"2","scopus_import":"1","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"das_tickbox":"1","article_processing_charge":"Yes (in subscription journal)"},{"page":"382-395","OA_type":"hybrid","article_type":"original","type":"journal_article","abstract":[{"lang":"eng","text":"Three-dimensional (3D) printing has rapidly developed from a niche hobbyist activity into a widely accessible and indispensable technology across multiple scientific disciplines. Within microscopy, optical engineering laboratories and imaging core facilities, 3D printing enables creating customised solutions for sample holders, optical components and everyday laboratory tools that traditionally required specialised machining. By providing rapid prototyping, low-cost production and reproducibility, 3D printing facilitates innovation and efficiency in facility operations. This article provides a perspective on the possibilities, challenges, and practical aspects of implementing 3D printing within microscopy core facilities. Instead of providing technical review about 3D printing, we focus on service organisation, user engagement, resource management and community-driven repositories for design dissemination. Our aim is to share insights with those considering the implementation of 3D printing as a service for developing add-on components to ease the operation of different aspects of the machine-park driven services and those who are managing advanced instrumentation within research groups."}],"department":[{"_id":"Bio"}],"ddc":["600"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"short":"M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, G. Krens, Journal of Microscopy 302 (2026) 382–395.","mla":"Goudarzi, Mohammad, et al. “3D Printing in Core Facilities – Low Pain, High Gain.” Journal of Microscopy, vol. 302, no. 3, Wiley, 2026, pp. 382–95, doi:10.1111/jmi.70106.","chicago":"Goudarzi, Mohammad, Maximilian Schuster, Arthur Milberger, Manuel Gunkel, Stefan Terjung, and Gabriel Krens. “3D Printing in Core Facilities – Low Pain, High Gain.” Journal of Microscopy. Wiley, 2026. https://doi.org/10.1111/jmi.70106.","apa":"Goudarzi, M., Schuster, M., Milberger, A., Gunkel, M., Terjung, S., & Krens, G. (2026). 3D printing in core facilities – Low pain, high gain. Journal of Microscopy. Wiley. https://doi.org/10.1111/jmi.70106","ista":"Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 2026. 3D printing in core facilities – Low pain, high gain. Journal of Microscopy. 302(3), 382–395.","ieee":"M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, and G. Krens, “3D printing in core facilities – Low pain, high gain,” Journal of Microscopy, vol. 302, no. 3. Wiley, pp. 382–395, 2026.","ama":"Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 3D printing in core facilities – Low pain, high gain. Journal of Microscopy. 2026;302(3):382-395. doi:10.1111/jmi.70106"},"publisher":"Wiley","author":[{"full_name":"Goudarzi, Mohammad","first_name":"Mohammad","id":"3384113A-F248-11E8-B48F-1D18A9856A87","last_name":"Goudarzi"},{"last_name":"Schuster","id":"37e65def-d415-11eb-ae59-a7b67be103db","first_name":"Maximilian","full_name":"Schuster, Maximilian"},{"last_name":"Milberger","first_name":"Arthur","full_name":"Milberger, Arthur"},{"full_name":"Gunkel, Manuel","first_name":"Manuel","last_name":"Gunkel"},{"first_name":"Stefan","full_name":"Terjung, Stefan","last_name":"Terjung"},{"first_name":"Gabriel","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","orcid":"0000-0003-4761-5996"}],"doi":"10.1111/jmi.70106","pmid":1,"oa_version":"Published Version","OA_place":"publisher","status":"public","researchdata_availability":"no","external_id":{"pmid":["42104760"]},"title":"3D printing in core facilities – Low pain, high gain","date_published":"2026-06-01T00:00:00Z","PlanS_conform":"1","acknowledgement":"This work was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Imaging & Optics Facility (IOF) and the MiBa Machine Shop. Specifically; Robert Hauschild (IOF), sharing designs, insights and pioneering 3D printing activities at the Imaging and Optics Facility; Bernhard Hochreiter (IOF), for support and testing of anoxic chamber. We also thank Ana Rita Carvalho Faria and Oliver Biehlmaier (Biozentrum University of Basel, Imaging Core Facility) for sharing the design of the adopted power meter.\r\nOpen Access funding provided by Institute of Science and Technology Austria.","has_accepted_license":"1","publication_status":"published","oa":1,"file_date_updated":"2026-07-27T14:01:34Z","supplementarymaterial":"no","volume":302,"date_updated":"2026-07-27T14:02:46Z","publication":"Journal of Microscopy","month":"06","quality_controlled":"1","year":"2026","date_created":"2026-05-17T22:02:11Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"M-Shop"}],"corr_author":"1","file":[{"checksum":"06dfad92b1465ed614a1201b4129960a","content_type":"application/pdf","access_level":"open_access","relation":"main_file","date_created":"2026-07-27T14:01:34Z","file_size":4625767,"date_updated":"2026-07-27T14:01:34Z","file_id":"22593","success":1,"creator":"dernst","file_name":"2026_JourMicroscopy_Goudarzi.pdf"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"_id":"21883","language":[{"iso":"eng"}],"intvolume":" 302","day":"01","publication_identifier":{"issn":["0022-2720"],"eissn":["1365-2818"]},"scopus_import":"1","issue":"3","das_tickbox":"0","article_processing_charge":"Yes (via OA deal)"},{"oa_version":"None","doi":"10.1177/02611929261453330","pmid":1,"author":[{"last_name":"Ulman","full_name":"Ulman, Yesim Isil","first_name":"Yesim Isil"},{"full_name":"Kostomitsopoulos, Nikos","first_name":"Nikos","last_name":"Kostomitsopoulos"},{"last_name":"Camenzind","full_name":"Camenzind, Samuel","first_name":"Samuel"},{"last_name":"Kitsara","full_name":"Kitsara, Maria","first_name":"Maria"},{"last_name":"Pavone","full_name":"Pavone, Ilja Richard","first_name":"Ilja Richard"},{"first_name":"Sophie","full_name":"Schober, Sophie","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","last_name":"Schober"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"SAGE Publications","citation":{"chicago":"Ulman, Yesim Isil, Nikos Kostomitsopoulos, Samuel Camenzind, Maria Kitsara, Ilja Richard Pavone, and Sophie Schober. “Emerging Bioethical Conflicts: One Health and Animal Experimentation.” Alternatives to Laboratory Animals. SAGE Publications, 2026. https://doi.org/10.1177/02611929261453330.","short":"Y.I. Ulman, N. Kostomitsopoulos, S. Camenzind, M. Kitsara, I.R. Pavone, S. Schober, Alternatives to Laboratory Animals 54 (2026) 226–235.","mla":"Ulman, Yesim Isil, et al. “Emerging Bioethical Conflicts: One Health and Animal Experimentation.” Alternatives to Laboratory Animals, vol. 54, no. 4, SAGE Publications, 2026, pp. 226–35, doi:10.1177/02611929261453330.","ama":"Ulman YI, Kostomitsopoulos N, Camenzind S, Kitsara M, Pavone IR, Schober S. Emerging bioethical conflicts: One Health and animal experimentation. Alternatives to Laboratory Animals. 2026;54(4):226-235. doi:10.1177/02611929261453330","apa":"Ulman, Y. I., Kostomitsopoulos, N., Camenzind, S., Kitsara, M., Pavone, I. R., & Schober, S. (2026). Emerging bioethical conflicts: One Health and animal experimentation. Alternatives to Laboratory Animals. SAGE Publications. https://doi.org/10.1177/02611929261453330","ista":"Ulman YI, Kostomitsopoulos N, Camenzind S, Kitsara M, Pavone IR, Schober S. 2026. Emerging bioethical conflicts: One Health and animal experimentation. Alternatives to Laboratory Animals. 54(4), 226–235.","ieee":"Y. I. Ulman, N. Kostomitsopoulos, S. Camenzind, M. Kitsara, I. R. Pavone, and S. Schober, “Emerging bioethical conflicts: One Health and animal experimentation,” Alternatives to Laboratory Animals, vol. 54, no. 4. SAGE Publications, pp. 226–235, 2026."},"date_published":"2026-07-01T00:00:00Z","title":"Emerging bioethical conflicts: One Health and animal experimentation","external_id":{"pmid":["42185081"]},"status":"public","article_type":"original","page":"226-235","OA_type":"closed access","department":[{"_id":"PreCl"}],"type":"journal_article","abstract":[{"text":"One Health initiatives are modern paradigms for research and health care practices in various fields. Concrete definitions of the One Health framework, however, remain heterogeneous, leading to conceptual problems and uncertainties in the application of the framework. This article discusses several approaches to the One Health concept, and their associated consequences, with special focus on animal experimentation. The first issue addressed is how One Health should be defined, as well as what (and who) should be considered within a One Health approach. In order to shed further light on this, we explore the history of animals in biomedical science, highlighting historical milestones in the use of animal models, as well as the development and current state of ethical considerations in the field of animal experimentation. The second issue comes with the inclusion of animal experimentation per se as part of the One Health concept. Therefore, particular attention is paid to bioethical principles and the resulting problems that can arise when applying them to the One Health concept. Arguments such as the idea of inequality between humans and non-human animals, and the premise that all actions are done for the benefit of humans, are raised and then used to explore the question of whether the One Health concept is compatible with existing bioethical principles. Based on the bioethical principles of protecting the environment, the biodiversity and biosphere, this paper seeks an inclusive perspective of the One Health concept. Successful solutions will be based on this concept, which embraces all living beings. The authors conclude that a multispecies ethics approach could help create a more ethical ecosystem that is aligned with the wellbeing of all life on a shared planet.","lang":"eng"}],"date_updated":"2026-07-27T14:13:17Z","volume":54,"quality_controlled":"1","month":"07","publication":"Alternatives to Laboratory Animals","publication_status":"published","day":"01","intvolume":" 54","_id":"21950","language":[{"iso":"eng"}],"corr_author":"1","date_created":"2026-06-07T22:01:36Z","year":"2026","article_processing_charge":"No","issue":"4","scopus_import":"1","publication_identifier":{"eissn":["2632-3559"],"issn":["0261-1929"]},"das_tickbox":"1"},{"day":"07","intvolume":" 34","_id":"20858","language":[{"iso":"eng"}],"date_created":"2025-12-28T23:01:26Z","year":"2026","main_file_link":[{"url":"https://doi.org/10.1101/2025.05.05.652195","open_access":"1"}],"article_processing_charge":"No","publication_identifier":{"eissn":["1525-0024"],"issn":["1525-0016"]},"issue":"1","scopus_import":"1","researchdata_availability":"upon request","status":"public","title":"Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response","date_published":"2026-01-07T00:00:00Z","external_id":{"biorxivid":["10.1101/2025.05.05.652195"]},"author":[{"last_name":"Rica","first_name":"Ramona","full_name":"Rica, Ramona"},{"last_name":"Klein","full_name":"Klein, Klara","first_name":"Klara"},{"full_name":"Johnson, Litty","first_name":"Litty","last_name":"Johnson"},{"last_name":"Carta","first_name":"Gabriele","full_name":"Carta, Gabriele"},{"first_name":"Mirza","full_name":"Sarcevic, Mirza","last_name":"Sarcevic"},{"id":"3C1BE782-F248-11E8-B48F-1D18A9856A87","last_name":"Langer","first_name":"Freyja","full_name":"Langer, Freyja"},{"full_name":"Rademacher, Christoph","first_name":"Christoph","last_name":"Rademacher"},{"last_name":"Wawrzinek","full_name":"Wawrzinek, Robert","first_name":"Robert"},{"last_name":"Quattrone","first_name":"Federica","full_name":"Quattrone, Federica"},{"first_name":"Florian","full_name":"Sparber, Florian","last_name":"Sparber"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Elsevier","citation":{"chicago":"Rica, Ramona, Klara Klein, Litty Johnson, Gabriele Carta, Mirza Sarcevic, Freyja Langer, Christoph Rademacher, Robert Wawrzinek, Federica Quattrone, and Florian Sparber. “Langerhans Cell-Targeted Protein Delivery Enhances Antigen-Specific Cellular Immune Response.” Molecular Therapy. Elsevier, 2026. https://doi.org/10.1016/j.ymthe.2025.10.008.","short":"R. Rica, K. Klein, L. Johnson, G. Carta, M. Sarcevic, F. Langer, C. Rademacher, R. Wawrzinek, F. Quattrone, F. Sparber, Molecular Therapy 34 (2026) 397–406.","mla":"Rica, Ramona, et al. “Langerhans Cell-Targeted Protein Delivery Enhances Antigen-Specific Cellular Immune Response.” Molecular Therapy, vol. 34, no. 1, Elsevier, 2026, pp. 397–406, doi:10.1016/j.ymthe.2025.10.008.","ama":"Rica R, Klein K, Johnson L, et al. Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response. Molecular Therapy. 2026;34(1):397-406. doi:10.1016/j.ymthe.2025.10.008","ieee":"R. Rica et al., “Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response,” Molecular Therapy, vol. 34, no. 1. Elsevier, pp. 397–406, 2026.","apa":"Rica, R., Klein, K., Johnson, L., Carta, G., Sarcevic, M., Langer, F., … Sparber, F. (2026). Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response. Molecular Therapy. Elsevier. https://doi.org/10.1016/j.ymthe.2025.10.008","ista":"Rica R, Klein K, Johnson L, Carta G, Sarcevic M, Langer F, Rademacher C, Wawrzinek R, Quattrone F, Sparber F. 2026. Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response. Molecular Therapy. 34(1), 397–406."},"OA_place":"repository","biorxivid":1,"oa_version":"Preprint","doi":"10.1016/j.ymthe.2025.10.008","department":[{"_id":"PreCl"}],"abstract":[{"text":"Targeted antigen delivery to immune cells, particularly dendritic cells, has emerged as a promising strategy to enhance therapeutic efficacy of vaccines, while minimizing adverse effects associated with conventional immunization. In this study, we use our previously described small glycomimetic molecule that is selectively recognized by the Langerhans cell (LC)-specific surface receptor Langerin and demonstrate specific delivery of protein antigens to these specialized dendritic cells. Our results show that Langerin-mediated antigen delivery significantly enhances the immune response in vivo, resulting in increased expansion and activation of antigen-specific T cells, compared to immunization with unmodified antigen. We demonstrate the feasibility of our LC-targeted platform for immune cell-specific immunization with protein antigen and underscore the potential of LCs as an access point for next-generation vaccines and immunotherapies.","lang":"eng"}],"type":"journal_article","dataavailabilitystatement":"The data that support the findings of this study are available on request from the corresponding authors.","article_type":"original","OA_type":"green","page":"397-406","publication":"Molecular Therapy","month":"01","quality_controlled":"1","volume":34,"date_updated":"2026-07-28T07:37:08Z","publication_status":"published","supplementarymaterial":"yes","oa":1,"acknowledgement":"This project was generously supported by Seedfinancing (grant no. P2282679) of the Austrian Bundesministerium für Digitalisierung und Wirtschaftsstandort and the Bundesministerium für Klimaschutz, Umwelt, Energie, Mobilität, Innovation, und Technologie, handled by the Austrian Wirtschaftsservice (aws), as well as by Life Science Call 2022 (grant no. FO999896442) of the Austrian Research Promotion Agency (FFG). We thank Mag. Michael Schunn from the PCF of the Institute of Science and Technology Austria for his continuous technical support."},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"year":"2026","date_created":"2026-07-29T09:10:35Z","intvolume":" 17","_id":"22608","language":[{"iso":"eng"}],"day":"17","related_material":{"record":[{"relation":"earlier_version","status":"public","id":"20465"}]},"corr_author":"1","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"file_name":"2026_NatureComm_Naik.pdf","date_updated":"2026-07-29T10:27:25Z","success":1,"file_id":"22609","creator":"dernst","relation":"main_file","date_created":"2026-07-29T10:27:25Z","file_size":15363936,"access_level":"open_access","checksum":"f26d96e180c1d034d9c9c8f57c3c258b","content_type":"application/pdf"}],"scopus_import":"1","publication_identifier":{"eissn":["2041-1723"]},"das_tickbox":"1","article_processing_charge":"Yes","project":[{"_id":"8f060199-16d5-11f0-9cad-f3253b266c46","grant_number":"PAT 5044023","name":"Keratins in epithelial tissue spreading"},{"call_identifier":"FWF","name":"Nano-Analytics of Cellular Systems","grant_number":"W1250-B20","_id":"252C3B08-B435-11E9-9278-68D0E5697425"}],"OA_type":"gold","article_type":"original","dataavailabilitystatement":"The authors declare that the minimum dataset that is necessary to\r\ninterpret, verify, and extend the research in this article is included in\r\nthe supplementary information, the source data, and the archived data\r\nrepository (https://doi.org/10.15479/AT-ISTA-21137). This is also available\r\non GitHub at https://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data. Source data are provided\r\nwith this paper. The framework to develop the vertex models used in this paper are\r\navailable online on GitHub and archived in the source data provided.\r\nCustom scripts used for analysis of imaging and simulation data are\r\nprovided along with data files for all panels in the source data for this\r\nmanuscript on GitHub and in data repo (https://doi.org/10.15479/ATISTA-\r\n21137). Framework for the vertex model is available at https://\r\ngithub.com/yketta/cells. Code for analysis is available on GitHub\r\nhttps://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data.","abstract":[{"lang":"eng","text":"For tissues to spread, they must deform while staying intact. How spreading tissues balance flexibility with integrity is not yet well understood. Here, we show that keratin intermediate filaments adapt tissue mechanical resilience to the stresses arising in epithelial tissues during spreading. By analyzing the expansion of the enveloping cell layer (EVL) over the yolk cell in zebrafish embryos in vivo, we find that keratin network maturation in EVL cells is promoted by stresses building up within the spreading tissue. Through genetic interference and tissue rheology experiments, complemented by a vertex model with mechanochemical feedback, we demonstrate that stress-induced keratin network maturation in the EVL increases tissue viscosity, to prevent tissue rupture. Further, keratins are required in the yolk cell for mechanosensitive actomyosin network contraction and flow, the forces pulling the EVL. These dual mechanosensitive functions of keratins enable a balance between pulling force production and EVL mechanical resilience, ensuring uniform and robust tissue spreading."}],"type":"journal_article","department":[{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EdHa"}],"ddc":["570"],"doi":"10.1038/s41467-026-72366-z","pmid":1,"oa_version":"Published Version","OA_place":"publisher","publisher":"Springer Nature","citation":{"ama":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. Nature Communications. 2026;17. doi:10.1038/s41467-026-72366-z","ieee":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P. J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties,” Nature Communications, vol. 17. Springer Nature, 2026.","ista":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. 2026. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. Nature Communications. 17, 6499.","apa":"Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., & Heisenberg, C.-P. J. (2026). Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-026-72366-z","chicago":"Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo, Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” Nature Communications. Springer Nature, 2026. https://doi.org/10.1038/s41467-026-72366-z.","short":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J. Heisenberg, Nature Communications 17 (2026).","mla":"Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” Nature Communications, vol. 17, 6499, Springer Nature, 2026, doi:10.1038/s41467-026-72366-z."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Naik","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8421-5508","full_name":"Naik, Suyash","first_name":"Suyash"},{"full_name":"Keta, Yann-Edwin","first_name":"Yann-Edwin","last_name":"Keta"},{"first_name":"Kornelija","full_name":"Pranjic-Ferscha, Kornelija","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","last_name":"Pranjic-Ferscha"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561","first_name":"Edouard B","full_name":"Hannezo, Edouard B"},{"first_name":"Silke","full_name":"Henkes, Silke","last_name":"Henkes"},{"first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"}],"external_id":{"pmid":["42143048"]},"article_number":"6499","title":"Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties","date_published":"2026-07-17T00:00:00Z","researchdata_availability":"yes","status":"public","has_accepted_license":"1","acknowledgement":"We thank all members of the Heisenberg, Henkes, and Hannezo groups for their support. We are also grateful to the Imaging and Optics, Scientific Computing, Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their technical assistance and support. Numerical simulations were performed using the computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020 research and innovation programme (grant agreement No. 665385). This work was supported by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to C.-P.H.","PlanS_conform":"1","oa":1,"supplementarymaterial":"yes","file_date_updated":"2026-07-29T10:27:25Z","publication_status":"published","date_updated":"2026-07-29T10:33:31Z","volume":17,"month":"07","quality_controlled":"1","publication":"Nature Communications"},{"OA_type":"green","article_type":"original","dataavailabilitystatement":"The data that support the findings of this study are openly available in Institute of Science and Technology repository at http://doi.org/10.15479/AT-ISTA-22242, Ref. 50.","abstract":[{"lang":"eng","text":"Planar germanium is currently the only semiconducting platform where high-coherence spin qubits and proximity-induced superconductivity have each been demonstrated. Recent research into spin qubits in Ge/SiGe heterostructures has focused on increasing the thickness of the SiGe capping layer, reporting improvements in the electrostatic noise levels. Meanwhile, heterostructures with thinner capping layers remain rather unexplored, despite the potential advantages for proximity-induced superconductivity. Here, we study a Ge/SiGe heterostructure with a thin SiGe cap d - 4nm and investigate its viability to host low-noise quantum dots. To keep the thermal budget compatible with superconducting layers, low-temperature oxide deposition processes were developed and implemented for the gate dielectrics. The charge noise level of the fabricated devices is estimated to be 1.8 +- 1.0 μeV/ square HZ, comparable to devices fabricated on shallow heterostructures ( d - 20nm) with high-temperature deposited oxides. Low charge noise levels, together with the straightforward integration of superconductors, make this heterostructure an attractive platform for prototyping hybrid semiconducting–superconducting devices."}],"type":"journal_article","department":[{"_id":"GeKa"},{"_id":"GradSch"},{"_id":"NanoFab"}],"doi":"10.1063/5.0333142","oa_version":"Preprint","OA_place":"repository","citation":{"ista":"Borovkov M, Schell YA, Sokolova D, Roux KER, Falthansl-Scheinecker P, Fabris G, Shah DC, Saez Mollejo J, Previdi R, Taha I, Genç A, Arbiol J, Calcaterra S, Oliveira ADC, Chrastina D, Isella G, Bubis A, Katsaros G. 2026. Low-noise quantum dots in ultra-shallow Ge/SiGe heterostructures for prototyping hybrid semiconducting–superconducting devices. Applied Physics Letters. 129(3), 033505.","apa":"Borovkov, M., Schell, Y. A., Sokolova, D., Roux, K. E. R., Falthansl-Scheinecker, P., Fabris, G., … Katsaros, G. (2026). Low-noise quantum dots in ultra-shallow Ge/SiGe heterostructures for prototyping hybrid semiconducting–superconducting devices. Applied Physics Letters. AIP Publishing. https://doi.org/10.1063/5.0333142","ieee":"M. Borovkov et al., “Low-noise quantum dots in ultra-shallow Ge/SiGe heterostructures for prototyping hybrid semiconducting–superconducting devices,” Applied Physics Letters, vol. 129, no. 3. AIP Publishing, 2026.","ama":"Borovkov M, Schell YA, Sokolova D, et al. Low-noise quantum dots in ultra-shallow Ge/SiGe heterostructures for prototyping hybrid semiconducting–superconducting devices. Applied Physics Letters. 2026;129(3). doi:10.1063/5.0333142","mla":"Borovkov, Maksim, et al. “Low-Noise Quantum Dots in Ultra-Shallow Ge/SiGe Heterostructures for Prototyping Hybrid Semiconducting–Superconducting Devices.” Applied Physics Letters, vol. 129, no. 3, 033505, AIP Publishing, 2026, doi:10.1063/5.0333142.","short":"M. Borovkov, Y.A. Schell, D. Sokolova, K.E.R. Roux, P. Falthansl-Scheinecker, G. Fabris, D.C. Shah, J. Saez Mollejo, R. Previdi, I. Taha, A. Genç, J. Arbiol, S. Calcaterra, A.D.C. Oliveira, D. Chrastina, G. Isella, A. Bubis, G. Katsaros, Applied Physics Letters 129 (2026).","chicago":"Borovkov, Maksim, Yona A Schell, Dina Sokolova, Kevin Etienne Robert Roux, Paul Falthansl-Scheinecker, Giorgio Fabris, Devashish C Shah, et al. “Low-Noise Quantum Dots in Ultra-Shallow Ge/SiGe Heterostructures for Prototyping Hybrid Semiconducting–Superconducting Devices.” Applied Physics Letters. AIP Publishing, 2026. https://doi.org/10.1063/5.0333142."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"AIP Publishing","author":[{"first_name":"Maksim","full_name":"Borovkov, Maksim","id":"1fd0975f-8b61-11ed-b69e-d149334f28c5","last_name":"Borovkov"},{"first_name":"Yona A","full_name":"Schell, Yona A","id":"fe39122d-06bb-11ec-a33b-9e22b40e40a5","last_name":"Schell"},{"full_name":"Sokolova, Dina","first_name":"Dina","last_name":"Sokolova","id":"2d2d62f8-72f0-11ef-b75a-8ec3e8a60032"},{"full_name":"Roux, Kevin Etienne Robert","first_name":"Kevin Etienne Robert","id":"53f93ea2-803f-11ed-ab7e-b283135794ef","last_name":"Roux"},{"full_name":"Falthansl-Scheinecker, Paul","first_name":"Paul","id":"85b43b21-15b2-11ec-abd3-e2c252cc2285","last_name":"Falthansl-Scheinecker"},{"last_name":"Fabris","id":"298cf6f3-1ff6-11ee-9fa6-d94cfa0b3352","first_name":"Giorgio","full_name":"Fabris, Giorgio"},{"full_name":"Shah, Devashish C","first_name":"Devashish C","orcid":"0009-0007-5829-7707","last_name":"Shah","id":"de191434-4e7e-11ef-bf4b-9a056fc19fc3"},{"full_name":"Saez Mollejo, Jaime","first_name":"Jaime","id":"e0390f72-f6e0-11ea-865d-862393336714","last_name":"Saez Mollejo"},{"last_name":"Previdi","id":"bc4ea1dc-00ce-11ec-8a4e-b325ca8b9876","first_name":"Rodolfo","full_name":"Previdi, Rodolfo"},{"full_name":"Taha, Inas","first_name":"Inas","last_name":"Taha"},{"first_name":"Aziz","full_name":"Genç, Aziz","last_name":"Genç"},{"last_name":"Arbiol","full_name":"Arbiol, Jordi","first_name":"Jordi"},{"full_name":"Calcaterra, Stefano","first_name":"Stefano","last_name":"Calcaterra"},{"full_name":"Oliveira, Afonso De Cerdeira","first_name":"Afonso De Cerdeira","last_name":"Oliveira"},{"full_name":"Chrastina, Daniel","first_name":"Daniel","last_name":"Chrastina"},{"last_name":"Isella","first_name":"Giovanni","full_name":"Isella, Giovanni"},{"first_name":"Anton","full_name":"Bubis, Anton","id":"1f6212b5-f795-11ec-9c0c-de4780302890","last_name":"Bubis"},{"id":"38DB5788-F248-11E8-B48F-1D18A9856A87","last_name":"Katsaros","orcid":"0000-0001-8342-202X","first_name":"Georgios","full_name":"Katsaros, Georgios"}],"external_id":{"arxiv":["2602.21363"]},"date_published":"2026-07-20T00:00:00Z","article_number":"033505","title":"Low-noise quantum dots in ultra-shallow Ge/SiGe heterostructures for prototyping hybrid semiconducting–superconducting devices","researchdata_availability":"yes","status":"public","acknowledgement":"We sincerely thank Nick van Loo, Greg Mazur, Dhananjay Joshi, and Srijit Goswami for their inputs on low-temperature HfOx deposition; Matias Urdampilleta and Daniel Jirovec for discussions; and Kristen Léonard for the careful reading of the manuscript. This research was supported by the Scientific Service Units of ISTA through resources provided by the Miba Machine Shop and the Nanofabrication facility. The authors acknowledge support from the NOMIS Foundation; the European Innovation Council Pathfinder Grant No. 101115315 (QuKiT); the FWF Projects with DOI:10.55776/F86, DOI:10.55776/PAT7682124, and DOI:10.55776/P36507; and the HE-MSCA-PF project with DOI:10.3030/101150858. ICN2 is supported by the Severo Ochoa Program from Spanish MCIN/AEI (Grant No.: CEX2021-001214-S) and is funded by the CERCA Program/Generalitat de Catalunya. ICN2 acknowledges funding from Generalitat de Catalunya (No. 2021SGR00457). We acknowledge support from the CSIC Interdisciplinary Thematic Platform (PTI+) on Quantum Technologies (PTI-QTEP+).","oa":1,"supplementarymaterial":"yes","publication_status":"published","date_updated":"2026-08-03T11:08:39Z","volume":129,"arxiv":1,"quality_controlled":"1","month":"07","publication":"Applied Physics Letters","main_file_link":[{"open_access":"1","url":"https://doi.org/10.48550/arXiv.2602.21363"}],"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"}],"date_created":"2026-08-02T22:01:53Z","year":"2026","language":[{"iso":"eng"}],"_id":"22619","intvolume":" 129","day":"20","corr_author":"1","related_material":{"record":[{"id":"22242","status":"public","relation":"research_data"}]},"scopus_import":"1","issue":"3","publication_identifier":{"issn":["0003-6951"],"eissn":["1077-3118"]},"das_tickbox":"1","article_processing_charge":"No","project":[{"grant_number":"101115315","_id":"bdc2ca30-d553-11ed-ba76-cf164a5bb811","name":"Quantum bits with Kitaev Transmons"},{"_id":"34a66131-11ca-11ed-8bc3-a31681c6b03e","grant_number":"F8606","name":"Center for Correlated Quantum Materials and Solid State Quantum Systems: Conventional and unconventional topological superconductors"},{"name":"Superconducting spin qubits in planar Ge","grant_number":"PAT 7682124","_id":"5b9e579c-ab3d-11f0-914f-88754c5b5a3f"},{"name":"Merging spin and superconducting qubits in planar Ge","_id":"bd8bd29e-d553-11ed-ba76-f0070d4b237a","grant_number":"P36507"},{"_id":"8ea8abf7-16d5-11f0-9cad-c41e56ec8bb3","grant_number":"101150858","name":"Realization and Manipulation of a Planar hybrid superconducting Andreev spin qubit in Germanium"}]},{"acknowledgement":"ISTA and the Werner Siemens Foundation financially supported this work. The Scientific Service Units (SSU) of ISTA supported this research through resources provided by the Electron Microscopy Facility (EMF), NMR Facility, and the Lab Support Facility (LSF). M.E. acknowledges financial support from Deutsche Forschungsgemeinschaft through Collaborative Research Centre 1411. We thank Dr. Tommaso Constanzo and Tobias Kleinhanns for assistance with high-quality electron microscope image acquisition, Dr. Jeonghyun Park for providing NCs, Dr. Mariano Calcabrini for assistance with the NMR study, and Prof. Jonathan De Roo for fruitful discussions. This work benefited from the use of the SasView application, originally developed under NSF award DMR-0520547. SasView contains code developed with funding from the European Union’s Horizon 2020 research and innovation program under the SINE2020 project, grant agreement No. 654000.","has_accepted_license":"1","PlanS_conform":"1","oa":1,"supplementarymaterial":"yes","file_date_updated":"2026-08-04T06:40:17Z","publication_status":"published","date_updated":"2026-08-04T06:47:13Z","volume":148,"month":"07","quality_controlled":"1","publication":"Journal of the AmericanChemical Society","page":"31245-31252","OA_type":"hybrid","article_type":"original","type":"journal_article","abstract":[{"text":"Nanocrystal superlattices are commonly formed by changing concentration, solvent conditions, or particle surface chemistry. Although effective, these approaches alter multiple contributions to the interparticle potential simultaneously, making it difficult to isolate the interactions responsible for ordering or to control assembly in chemically complex environments. Here, we show that oligomeric species present in a nanocrystal reaction medium drive superlattice formation through a depletion mechanism. Using PbTe nanocrystals as a model system, we identify Pb–oleate oligomers in the crude reaction mixture, characterize their solution structure, and quantify their contribution to the interparticle potential, establishing depletion as the dominant short-range interaction governing spontaneous body-centered cubic superlattice formation. We then confirm the depletion origin of ordering by showing that varying depletant concentration predictably shifts the order–disorder boundary and produces a thermally reversible transition between dispersed and ordered states ─ behavior that is inconsistent with van der Waals or ligand-mediated mechanisms but is a direct consequence of depletion control. Having established and validated the mechanism, we demonstrate that the same depletion framework can be deliberately activated in purified dispersions and transferred across nanocrystal systems of different composition and shape, including anisotropic and binary assemblies. These results establish precursor-derived depletion as a general and chemically grounded mechanism for nanocrystal superlattice formation, and show that collective ordering can be programmed through the surrounding medium rather than through particle surface modification.","lang":"eng"}],"ddc":["540"],"department":[{"_id":"MaIb"},{"_id":"LifeSc"},{"_id":"GradSch"},{"_id":"CaGo"}],"pmid":1,"doi":"10.1021/jacs.6c07859","OA_place":"publisher","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Lee, Seungho, Daniel Balazs, Aiswarya Rayaroth Puthiyaveettil, Sharona Horta, Carl Peter Goodrich, Michael Engel, Ihor Cherniukh, and Maria Ibáñez. “Reaction Medium Asan Architect of Nanocrystal Superlattices.” Journal of the AmericanChemical Society. American Chemical Society, 2026. https://doi.org/10.1021/jacs.6c07859.","mla":"Lee, Seungho, et al. “Reaction Medium Asan Architect of Nanocrystal Superlattices.” Journal of the AmericanChemical Society, vol. 148, no. 29, American Chemical Society, 2026, pp. 31245–52, doi:10.1021/jacs.6c07859.","short":"S. Lee, D. Balazs, A. Rayaroth Puthiyaveettil, S. Horta, C.P. Goodrich, M. Engel, I. Cherniukh, M. Ibáñez, Journal of the AmericanChemical Society 148 (2026) 31245–31252.","ama":"Lee S, Balazs D, Rayaroth Puthiyaveettil A, et al. Reaction medium asan architect of nanocrystal superlattices. Journal of the AmericanChemical Society. 2026;148(29):31245-31252. doi:10.1021/jacs.6c07859","apa":"Lee, S., Balazs, D., Rayaroth Puthiyaveettil, A., Horta, S., Goodrich, C. P., Engel, M., … Ibáñez, M. (2026). Reaction medium asan architect of nanocrystal superlattices. Journal of the AmericanChemical Society. American Chemical Society. https://doi.org/10.1021/jacs.6c07859","ista":"Lee S, Balazs D, Rayaroth Puthiyaveettil A, Horta S, Goodrich CP, Engel M, Cherniukh I, Ibáñez M. 2026. Reaction medium asan architect of nanocrystal superlattices. Journal of the AmericanChemical Society. 148(29), 31245–31252.","ieee":"S. Lee et al., “Reaction medium asan architect of nanocrystal superlattices,” Journal of the AmericanChemical Society, vol. 148, no. 29. American Chemical Society, pp. 31245–31252, 2026."},"publisher":"American Chemical Society","author":[{"full_name":"Lee, Seungho","first_name":"Seungho","orcid":"0000-0002-6962-8598","last_name":"Lee","id":"BB243B88-D767-11E9-B658-BC13E6697425"},{"full_name":"Balazs, Daniel","first_name":"Daniel","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","last_name":"Balazs","orcid":"0000-0001-7597-043X"},{"id":"8aceb01b-8972-11ed-ae7b-d5fe53775add","last_name":"Rayaroth Puthiyaveettil","first_name":"Aiswarya","full_name":"Rayaroth Puthiyaveettil, Aiswarya"},{"full_name":"Horta, Sharona","first_name":"Sharona","last_name":"Horta","id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc"},{"last_name":"Goodrich","id":"EB352CD2-F68A-11E9-89C5-A432E6697425","orcid":"0000-0002-1307-5074","full_name":"Goodrich, Carl Peter","first_name":"Carl Peter"},{"first_name":"Michael","full_name":"Engel, Michael","last_name":"Engel"},{"id":"d03b62b2-5976-11ef-a8d7-9525504b7895","last_name":"Cherniukh","full_name":"Cherniukh, Ihor","first_name":"Ihor"},{"full_name":"Ibáñez, Maria","first_name":"Maria","orcid":"0000-0001-5013-2843","last_name":"Ibáñez","id":"43C61214-F248-11E8-B48F-1D18A9856A87"}],"external_id":{"pmid":["42532904"]},"title":"Reaction medium asan architect of nanocrystal superlattices","date_published":"2026-07-15T00:00:00Z","status":"public","researchdata_availability":"no","scopus_import":"1","issue":"29","publication_identifier":{"issn":["0002-7863"],"eissn":["1520-5126"]},"das_tickbox":"0","article_processing_charge":"Yes (via OA deal)","project":[{"name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery","_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A"}],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"NMR"},{"_id":"LifeSc"}],"date_created":"2026-08-04T06:29:31Z","year":"2026","language":[{"iso":"eng"}],"_id":"22645","intvolume":" 148","day":"15","corr_author":"1","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"file_name":"2026_JACS_Lee.pdf","creator":"dernst","success":1,"file_id":"22646","date_updated":"2026-08-04T06:40:17Z","file_size":6564594,"date_created":"2026-08-04T06:40:17Z","relation":"main_file","checksum":"063314ae5ac4225ebd4436aa8707d113","content_type":"application/pdf","access_level":"open_access"}]},{"article_type":"original","OA_type":"hybrid","dataavailabilitystatement":"Single-nucleus multiomics data are available from the Gene Expression Omnibus (GSE328363). The mm10 reference genome was used for the alignment (refdata-cellranger-arc-mm10-2020-A-2.0.0, obtained from https://cf.10xgenomics.com/supp/cell-arc/refdata-cellranger-arc-mm10-2020-A-2.0.0.tar.gz). Single-cell data can be accessed and visualized through a CELLxGENE database (https://adameykolab.hifo.meduniwien.ac.at/cellxgene_public/filecrawl/.2026_Nature_Schwarz). Source data are provided with this paper. Scripts and analyses that support the main findings of this study are accessible in a GitHub repository (https://git.ista.ac.at/research-sofware/mouseome).","ddc":["570"],"department":[{"_id":"AnKi"},{"_id":"GaNo"},{"_id":"TiVo"},{"_id":"ScienComp"},{"_id":"GradSch"},{"_id":"Bio"},{"_id":"PreCl"}],"type":"journal_article","abstract":[{"lang":"eng","text":"Despite the functional diversity of over 100 causal genes1,2,3, phenotypic convergence across models may reveal common neurobiological processes in autism spectrum disorder (ASD). Here we profiled 251 samples from 11 monogenic mouse models of ASD using single-nucleus multi-omic sequencing across three developmental stages, both sexes and two brain regions. Despite genetic heterogeneity, ASD-linked mutations converged on perturbations of the radial glial cell lineage. These alterations reflect a transient developmental delay rather than lasting lineage misspecification and resolve by postnatal stages. Molecularly, the largest transcriptional differences emerged in neurons at early postnatal stages. These changes included downregulation of synaptic and ion channel-related genes, consistent with homeostatic adaptation or delayed maturation. Network analysis showed molecular convergence across models within each developmental stage, suggesting that diverse mutations linked to ASD impinge on common, stage-specific processes. Convergence becomes less pronounced by postnatal day 14, highlighting the dynamic nature of ASD-associated changes. Cross-genotype heterogeneity is superimposed on stage-specific effects. Electrophysiology corroborated this pattern: mutants generally showed altered neuronal excitability and synaptic properties with model-specific nuances. Our study also highlighted sex-specific gene expression alterations, with female mice often displaying larger effect sizes than male mice. Together, our findings provide a comprehensive view of developmental cellular and molecular dynamics across models of ASD."}],"OA_place":"publisher","oa_version":"Published Version","doi":"10.1038/s41586-026-10679-1","pmid":1,"author":[{"first_name":"Lena A","full_name":"Schwarz, Lena A","id":"29A8453C-F248-11E8-B48F-1D18A9856A87","last_name":"Schwarz"},{"last_name":"Dotter","id":"4C66542E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9033-9096","full_name":"Dotter, Christoph","first_name":"Christoph"},{"full_name":"Isaev, Sergey","first_name":"Sergey","last_name":"Isaev"},{"full_name":"Lisi, Michela","first_name":"Michela","id":"39383c1b-d3eb-11ef-8d6c-c8cdf4e10c8c","last_name":"Lisi"},{"full_name":"Malzl, Daniel","first_name":"Daniel","last_name":"Malzl"},{"first_name":"Christoph","full_name":"Büschl, Christoph","id":"2a8c054c-0913-11ee-9159-f8ef515809ed","last_name":"Büschl"},{"last_name":"Ladstätter","full_name":"Ladstätter, Sabrina","first_name":"Sabrina"},{"full_name":"Oliveira, Bárbara","first_name":"Bárbara","last_name":"Oliveira","id":"3B03AA1A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Matteo","full_name":"Barel, Matteo","id":"8959927b-2236-11ed-bd6e-ea83d94ade0e","last_name":"Barel"},{"orcid":"0000-0003-1843-3173","last_name":"Basilico","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","first_name":"Bernadette","full_name":"Basilico, Bernadette"},{"first_name":"Chaitanya","full_name":"Chintaluri, Chaitanya","orcid":"0000-0003-4252-1608","id":"BA06AFEE-A4BA-11EA-AE5C-14673DDC885E","last_name":"Chintaluri"},{"full_name":"Gorkiewicz, Sarah","first_name":"Sarah","id":"f141a35d-15a9-11ec-9fb2-fef6becc7b6f","last_name":"Gorkiewicz"},{"first_name":"Mohammad","full_name":"Goudarzi, Mohammad","last_name":"Goudarzi","id":"3384113A-F248-11E8-B48F-1D18A9856A87"},{"id":"0bf89b6a-d28b-11eb-8bd6-f43768e4d368","last_name":"Belinova","first_name":"Tereza","full_name":"Belinova, Tereza"},{"full_name":"Reichl, Stephan","first_name":"Stephan","last_name":"Reichl"},{"id":"dd6d52f2-c50d-11eb-9548-bcf0ff82b344","last_name":"Sendžikaitė","first_name":"Gintarė","full_name":"Sendžikaitė, Gintarė"},{"id":"b0bbee33-09f7-11eb-909c-8b358058d28a","last_name":"Arcot Jayaram","orcid":"0000-0002-2479-2669","first_name":"Satish","full_name":"Arcot Jayaram, Satish"},{"id":"3B8B25A8-F248-11E8-B48F-1D18A9856A87","last_name":"Koppensteiner","orcid":"0000-0002-3509-1948","first_name":"Peter","full_name":"Koppensteiner, Peter"},{"last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","first_name":"Christoph M","full_name":"Sommer, Christoph M"},{"first_name":"Tim P","full_name":"Vogels, Tim P","last_name":"Vogels","id":"CB6FF8D2-008F-11EA-8E08-2637E6697425","orcid":"0000-0003-3295-6181"},{"last_name":"Menche","full_name":"Menche, Jörg","first_name":"Jörg"},{"last_name":"Adameyko","first_name":"Igor","full_name":"Adameyko, Igor"},{"full_name":"Kharchenko, Peter Vasili","first_name":"Peter Vasili","last_name":"Kharchenko","id":"0095641e-7eb7-11f1-8665-aec51a2ab5e0"},{"first_name":"Christoph","full_name":"Bock, Christoph","last_name":"Bock"},{"orcid":"0000-0002-7673-7178","last_name":"Novarino","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","full_name":"Novarino, Gaia","first_name":"Gaia"}],"citation":{"mla":"Schwarz, Lena A., et al. “Cortical Development Dynamics across Autism Spectrum Disorder Mouse Models.” Nature, Springer Nature, 2026, doi:10.1038/s41586-026-10679-1.","short":"L.A. Schwarz, C. Dotter, S. Isaev, M. Lisi, D. Malzl, C. Büschl, S. Ladstätter, B. Oliveira, M. Barel, B. Basilico, C. Chintaluri, S. Gorkiewicz, M. Goudarzi, T. Belinova, S. Reichl, G. Sendžikaitė, S. Arcot Jayaram, P. Koppensteiner, C.M. Sommer, T.P. Vogels, J. Menche, I. Adameyko, P.V. Kharchenko, C. Bock, G. Novarino, Nature (2026).","chicago":"Schwarz, Lena A, Christoph Dotter, Sergey Isaev, Michela Lisi, Daniel Malzl, Christoph Büschl, Sabrina Ladstätter, et al. “Cortical Development Dynamics across Autism Spectrum Disorder Mouse Models.” Nature. Springer Nature, 2026. https://doi.org/10.1038/s41586-026-10679-1.","ieee":"L. A. Schwarz et al., “Cortical development dynamics across autism spectrum disorder mouse models,” Nature. Springer Nature, 2026.","apa":"Schwarz, L. A., Dotter, C., Isaev, S., Lisi, M., Malzl, D., Büschl, C., … Novarino, G. (2026). Cortical development dynamics across autism spectrum disorder mouse models. Nature. Springer Nature. https://doi.org/10.1038/s41586-026-10679-1","ista":"Schwarz LA, Dotter C, Isaev S, Lisi M, Malzl D, Büschl C, Ladstätter S, Oliveira B, Barel M, Basilico B, Chintaluri C, Gorkiewicz S, Goudarzi M, Belinova T, Reichl S, Sendžikaitė G, Arcot Jayaram S, Koppensteiner P, Sommer CM, Vogels TP, Menche J, Adameyko I, Kharchenko PV, Bock C, Novarino G. 2026. Cortical development dynamics across autism spectrum disorder mouse models. Nature.","ama":"Schwarz LA, Dotter C, Isaev S, et al. Cortical development dynamics across autism spectrum disorder mouse models. Nature. 2026. doi:10.1038/s41586-026-10679-1"},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publisher":"Springer Nature","date_published":"2026-06-17T00:00:00Z","title":"Cortical development dynamics across autism spectrum disorder mouse models","external_id":{"pmid":["42310454"]},"researchdata_availability":"yes","status":"public","acknowledgement":"We thank F. Freeman, V. Voronin and M. Ladron de Guevara for technical assistance; A. Stichelberger and S. Liegenfeld for the management of our animal colony; M. Schunn, C. Gold and the Preclinical Facility team for technical assistance; C. Jansen and the Scientific Computing Facility for bioinformatics support and technical assistance; the Biomedical Sequencing Facility at CeMM for assistance with next-generation sequencing; and J. Lin and T. Krausgruber in the laboratory of C. Bock for support with flow cytometry; J. Kirchner for illustrating the multi-omics approach depicted in Fig. 1; and all members of the laboratory of G.N. for their support and discussions. This study was supported by the Scientific Service Units of ISTA through resources provided by the Imaging & Optics Facility and the Laboratory Support Facility. Bulk RNA-seq was performed by the Next Generation Sequencing Facility at Vienna BioCenter Core Facilities, member of the Vienna BioCenter. This work was supported by a European Research Council Consolidator Grant (PR1028ERC02), by SFARI (PR1028SIM02) and by the Austrian Science Fund (PE1028W1232 and PR1028FG1803) to G.N. Open access funding provided by Institute of Science and Technology (IST Austria).","has_accepted_license":"1","PlanS_conform":"1","supplementarymaterial":"yes","oa":1,"publication_status":"epub_ahead","date_updated":"2026-08-04T09:29:55Z","quality_controlled":"1","month":"06","publication":"Nature","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41586-026-10679-1"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"date_created":"2026-07-13T09:47:21Z","year":"2026","day":"17","language":[{"iso":"eng"}],"_id":"22295","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"corr_author":"1","related_material":{"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/patterns-in-genetic-chaos/"}]},"scopus_import":"1","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"article_processing_charge":"Yes (via OA deal)","project":[{"_id":"34ba8964-11ca-11ed-8bc3-e15864e7e9a6","grant_number":"101044865","name":"Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders"},{"_id":"9B91375C-BA93-11EA-9121-9846C619BF3A","grant_number":"707964","name":"Critical windows and reversibility of ASD associated with mutations in chromatin remodelers"},{"call_identifier":"FWF","name":"Molecular Drug Targets","_id":"2548AE96-B435-11E9-9278-68D0E5697425","grant_number":"W1232"},{"name":"Neurobiology of anxiety in autism spectrum disorders","grant_number":"FG1803 49015","_id":"ebb38b5d-77a9-11ec-83b8-a42e08120a88"}]},{"PlanS_conform":"1","has_accepted_license":"1","acknowledgement":"We thank N. R. Skrynnikov and O. O. Lebedenko (St. Petersburg) for insightful discussions and for performing exploratory MD simulations. We are grateful to T. Schubeis (Lyon) for advice on GB1 crystallization and R. Schmid for initial crystallization trials. We thank C. Mueller-Dieckmann for assistance with room-temperature X-ray crystallography data collection on beamline ID30B at the ESRF, which is acknowledged for providing beamtime through its In-House Research programme. We thank S. Falkner for assistance with constructing the structural model of the IgG:GB1 complex. We thank J. Lewandowski for providing feedback on the paper and granting access to backbone relaxation data of IgG:GB1T2Q and GB1T2Q microcrystals. This research was supported by the Scientific Service Units (SSU) of the Institute of Science and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance and the Lab Support Facilities. We thank P. Rovó and M. V. Falcón for excellent support of the NMR facility. L.M.B. is recipient of a DOC fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology Austria (grant number PR10660EAW01). C.C. acknowledges the European Research Council (grant project 101097272 ‘MilliInMicro’) and the Métropole du Grand Nancy (grant project ‘ARC’). BM07-FIP2 is supported by the French ANR PIA3 (France 2030) EquipEx+ project MAGNIFIX under grant agreement ANR-21-ESRE-0011.Open access funding provided by Institute of Science and Technology (IST Austria).","publication_status":"published","oa":1,"supplementarymaterial":"yes","file_date_updated":"2026-07-28T06:58:35Z","volume":18,"date_updated":"2026-08-04T09:32:45Z","publication":"Nature Chemistry","quality_controlled":"1","month":"07","dataavailabilitystatement":"The cryo and room-temperature crystal structures of GB1QDD are deposited at the PDB under the access codes 9I2I and 9T8Z, respectively. The solid-state NMR backbone assignment of GB1QDD is deposited at the BMRB under the access code 53330. NMR spectra, analysis scripts and raw data are publicly available at the ISTA research explorer (https://doi.org/10.15479/AT-ISTA-20641)120. Files to reproduce the enhanced-sampling MD simulations are publicly available at the ISTA research explorer (https://doi.org/10.15479/AT-ISTA-21145)121.","OA_type":"hybrid","page":"1221-1230","article_type":"original","abstract":[{"text":"Protein conformational energy landscapes are shaped not only by intramolecular interactions but also by their environment. In protein crystals and protein–protein complexes, intermolecular contacts alter this energy landscape, but the exact nature of this alteration is difficult to decipher. Understanding how the crystal lattice affects protein dynamics is crucial for crystallography-based studies of motion, yet its influence on collective motions remains unclear. Aromatic ring flips in the hydrophobic core represent sensitive probes of such dynamics. Here, we compare the kinetics of aromatic ring flips in the protein GB1 in crystals, in complex with its binding partner IgG, and in solution, combining advanced isotope labelling with quantitative NMR methods. We show that rings in the core flip nearly a thousand times less frequently in crystals than in solution. Enhanced-sampling molecular dynamics simulations, based on a crystal structure of a GB1 variant reported in this work, reproduce these elevated barriers and reveal how the crystal restrains motions.","lang":"eng"}],"type":"journal_article","ddc":["540"],"department":[{"_id":"PaSc"},{"_id":"LifeSc"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publisher":"Springer Nature","citation":{"mla":"Becker, Lea Marie, et al. “Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.” Nature Chemistry, vol. 18, Springer Nature, 2026, pp. 1221–30, doi:10.1038/s41557-026-02155-0.","short":"L.M. Becker, H. Fu, B. Tatman, M. Dreydoppel, A. Kapitonova, D. Balazs, U. Weininger, S. Engilberge, C. Chipot, P. Schanda, Nature Chemistry 18 (2026) 1221–1230.","chicago":"Becker, Lea Marie, Haohao Fu, Benjamin Tatman, Matthias Dreydoppel, Anna Kapitonova, Daniel Balazs, Ulrich Weininger, Sylvain Engilberge, Christophe Chipot, and Paul Schanda. “Aromatic Ring Flips Reveal Reshaping of Protein Dynamics in Crystals and Complexes.” Nature Chemistry. Springer Nature, 2026. https://doi.org/10.1038/s41557-026-02155-0.","ieee":"L. M. Becker et al., “Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes,” Nature Chemistry, vol. 18. Springer Nature, pp. 1221–1230, 2026.","ista":"Becker LM, Fu H, Tatman B, Dreydoppel M, Kapitonova A, Balazs D, Weininger U, Engilberge S, Chipot C, Schanda P. 2026. Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes. Nature Chemistry. 18, 1221–1230.","apa":"Becker, L. M., Fu, H., Tatman, B., Dreydoppel, M., Kapitonova, A., Balazs, D., … Schanda, P. (2026). Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes. Nature Chemistry. Springer Nature. https://doi.org/10.1038/s41557-026-02155-0","ama":"Becker LM, Fu H, Tatman B, et al. Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes. Nature Chemistry. 2026;18:1221-1230. doi:10.1038/s41557-026-02155-0"},"author":[{"full_name":"Becker, Lea Marie","first_name":"Lea Marie","orcid":"0000-0002-6401-5151","id":"36336939-eb97-11eb-a6c2-c83f1214ca79","last_name":"Becker"},{"last_name":"Fu","full_name":"Fu, Haohao","first_name":"Haohao"},{"first_name":"Benjamin","full_name":"Tatman, Benjamin","id":"71cda2f3-e604-11ee-a1df-da10587eda3f","last_name":"Tatman"},{"first_name":"Matthias","full_name":"Dreydoppel, Matthias","last_name":"Dreydoppel"},{"first_name":"Anna","full_name":"Kapitonova, Anna","id":"9fb2a840-89e1-11ee-a8b7-cc5c7ba62471","last_name":"Kapitonova"},{"first_name":"Daniel","full_name":"Balazs, Daniel","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","last_name":"Balazs","orcid":"0000-0001-7597-043X"},{"first_name":"Ulrich","full_name":"Weininger, Ulrich","last_name":"Weininger"},{"first_name":"Sylvain","full_name":"Engilberge, Sylvain","last_name":"Engilberge"},{"full_name":"Chipot, Christophe","first_name":"Christophe","last_name":"Chipot"},{"first_name":"Paul","full_name":"Schanda, Paul","last_name":"Schanda","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","orcid":"0000-0002-9350-7606"}],"pmid":1,"doi":"10.1038/s41557-026-02155-0","oa_version":"Published Version","OA_place":"publisher","status":"public","researchdata_availability":"yes","external_id":{"pmid":["42271006"]},"title":"Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes","date_published":"2026-07-01T00:00:00Z","publication_identifier":{"eissn":["17554349"],"issn":["17554330"]},"scopus_import":"1","das_tickbox":"1","article_processing_charge":"Yes (via OA deal)","project":[{"name":"Exploring protein dynamics by solid-state MAS NMR through specific labeling approaches","_id":"7be609c4-9f16-11ee-852c-85015ce2b9b0","grant_number":"26777"}],"date_created":"2026-06-21T22:03:01Z","year":"2026","acknowledged_ssus":[{"_id":"NMR"},{"_id":"LifeSc"}],"related_material":{"record":[{"relation":"research_data","status":"public","id":"20641"},{"relation":"research_data","status":"public","id":"21145"},{"id":"22334","status":"public","relation":"dissertation_contains"}],"link":[{"url":"https://ista.ac.at/en/news/how-proteins-breathe/","relation":"research_data","description":"News on ISTA website"}]},"corr_author":"1","file":[{"file_name":"2026_NatureChemistry_Becker.pdf","date_updated":"2026-07-28T06:58:35Z","creator":"dernst","file_id":"22595","success":1,"relation":"main_file","file_size":2618184,"date_created":"2026-07-28T06:58:35Z","access_level":"open_access","checksum":"1069fb27949fd2cb641b043b3a96a580","content_type":"application/pdf"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"_id":"22105","language":[{"iso":"eng"}],"intvolume":" 18","day":"01"},{"publication_identifier":{"eissn":["2380-8195"]},"issue":"8","scopus_import":"1","das_tickbox":"0","article_processing_charge":"Yes (via OA deal)","project":[{"name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery","_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A"}],"year":"2026","date_created":"2026-08-18T11:34:03Z","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"NanoFab"},{"_id":"MassSpec"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"date_updated":"2026-08-19T05:52:41Z","creator":"dernst","success":1,"file_id":"22736","file_name":"2026_ACSEnergyLetters_Liu.pdf","content_type":"application/pdf","checksum":"4d75c5a79d112c845c9eecba8838db38","access_level":"open_access","relation":"main_file","file_size":6806815,"date_created":"2026-08-19T05:52:41Z"}],"corr_author":"1","day":"14","language":[{"iso":"eng"}],"_id":"22734","intvolume":" 11","PlanS_conform":"1","has_accepted_license":"1","acknowledgement":"Open access funding provided by Institute of Science and Technology Austria. M.I. acknowledges financial support from ISTA and the Werner Siemens Foundation. The Scientific Service Units (SSU) of ISTA supported this work through resources provided by the Electron Microscopy Facility (EMF), the Lab Support Facility (LSF), the Nanofabrication Facility (NNF), and the Mass Spectrometry Facility. Y.L. acknowledges funding from the National Natural Science Foundation of China (NSFC) (grant no. 22209034) and the Fundamental Research Funds for the Central Universities (JZ2024HGTB0239). M.H. acknowledges funding from Australian Research Council (FT230100316), and the high-performance computing resources provided by National Computational Infrastructure (it39) and Pawsey Supercomputing Centre (pawsey1075). ICN2 acknowledges funding from Generalitat de Catalunya 2021SGR00457. The authors thank support from the project AMaDE (PID2023-149158OB-C43), funded by MCIN/AEI/10.13039/501100011033/ and by the “ERDF Away of making Europe”, by the “European Union”. ICN2 is supported by the Severo Ochoa program from Spanish MCIN/AEI (grant no.: CEX2021-001214-S) and is funded by the CERCA Programme/Generalitat de Catalunya. Authors acknowledge the use of instrumentation as well as the technical advice provided by the Joint Electron Microscopy Center at ALBA (JEMCA). ICN2 acknowledges funding from Grant IU16-014206 (METCAM-FIB) funded by the European Union through the European Regional Development Fund (ERDF), with the support of the Ministry of Research and Universities, Generalitat de Catalunya. ICN2 is founding member of e-DREAM. (91)","publication_status":"published","file_date_updated":"2026-08-19T05:52:41Z","supplementarymaterial":"yes","oa":1,"volume":11,"date_updated":"2026-08-19T05:53:33Z","publication":"ACS Energy Letters","month":"08","quality_controlled":"1","article_type":"letter_note","OA_type":"hybrid","page":"5752-5762","department":[{"_id":"MassSpec"},{"_id":"MaIb"},{"_id":"GradSch"},{"_id":"EM-Fac"}],"ddc":["540"],"abstract":[{"lang":"eng","text":"Silver selenide (Ag2Se) is a promising near-room-temperature thermoelectric material, but its narrow stoichiometric window and β–α phase transition complicate reproducible microstructure control. Here, we present a mismatch-assisted microstructure engineering strategy in which Ag2Se particles are treated with polyanionic ZnSe complexes and consolidated through the β–α transition to introduce ZnSe nanoprecipitates, Ag2Se/ZnSe interfaces, and local strain fields. The crystallographic mismatch between ZnSe and Ag2Se, together with the Zn2+/Ag+ size difference, amplifies phase-transition-induced deformation and promotes high-density dislocations with periodic strain modulations. This defect architecture suppresses grain coarsening, removes excess Ag, limits Ag-interstitial formation, and reduces lattice thermal conductivity through lattice softening and multiscale phonon scattering. Ag2Se–4%ZnSe nanocomposites achieve a peak zTmax of 1.13 at 369 K and a zTavg of 1.08 from 300 to 380 K, demonstrating mismatch-driven defect engineering through the β–α phase transition as a route for optimizing Ag2Se-based thermoelectrics."}],"type":"journal_article","author":[{"last_name":"Liu","id":"2A70014E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7313-6740","full_name":"Liu, Yu","first_name":"Yu"},{"first_name":"Tobias","full_name":"Kleinhanns, Tobias","orcid":"0000-0003-1537-7436","last_name":"Kleinhanns","id":"8BD9DE16-AB3C-11E9-9C8C-2A03E6697425"},{"last_name":"Spadaro","first_name":"Maria Chiara","full_name":"Spadaro, Maria Chiara"},{"last_name":"Genç","full_name":"Genç, Aziz","first_name":"Aziz"},{"full_name":"Horta, Sharona","first_name":"Sharona","id":"03a7e858-01b1-11ec-8b71-99ae6c4a05bc","last_name":"Horta"},{"orcid":"0000-0001-7408-8197","last_name":"Navita","id":"6ebe278d-ba0b-11ee-8184-f34cdc671de4","full_name":"Navita, Navita","first_name":"Navita"},{"orcid":"0000-0001-9732-3815","id":"D93824F4-D9BA-11E9-BB12-F207E6697425","last_name":"Costanzo","full_name":"Costanzo, Tommaso","first_name":"Tommaso"},{"id":"0601cc46-c082-11ec-9b07-bb29641d1de9","last_name":"Dutkiewicz","full_name":"Dutkiewicz, Ewelina","first_name":"Ewelina"},{"last_name":"Arbiol","first_name":"Jordi","full_name":"Arbiol, Jordi"},{"last_name":"Hong","full_name":"Hong, Min","first_name":"Min"},{"first_name":"Maria","full_name":"Ibáñez, Maria","id":"43C61214-F248-11E8-B48F-1D18A9856A87","last_name":"Ibáñez","orcid":"0000-0001-5013-2843"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"American Chemical Society","citation":{"ama":"Liu Y, Kleinhanns T, Spadaro MC, et al. Exploiting mismatch strain and the β–α phase transition for microstructural engineering in thermoelectric Ag2Se. ACS Energy Letters. 2026;11(8):5752-5762. doi:10.1021/acsenergylett.6c01499","ieee":"Y. Liu et al., “Exploiting mismatch strain and the β–α phase transition for microstructural engineering in thermoelectric Ag2Se,” ACS Energy Letters, vol. 11, no. 8. American Chemical Society, pp. 5752–5762, 2026.","apa":"Liu, Y., Kleinhanns, T., Spadaro, M. C., Genç, A., Horta, S., Jakhar, N., … Ibáñez, M. (2026). Exploiting mismatch strain and the β–α phase transition for microstructural engineering in thermoelectric Ag2Se. ACS Energy Letters. American Chemical Society. https://doi.org/10.1021/acsenergylett.6c01499","ista":"Liu Y, Kleinhanns T, Spadaro MC, Genç A, Horta S, Jakhar N, Costanzo T, Dutkiewicz E, Arbiol J, Hong M, Ibáñez M. 2026. Exploiting mismatch strain and the β–α phase transition for microstructural engineering in thermoelectric Ag2Se. ACS Energy Letters. 11(8), 5752–5762.","chicago":"Liu, Yu, Tobias Kleinhanns, Maria Chiara Spadaro, Aziz Genç, Sharona Horta, Navita Jakhar, Tommaso Costanzo, et al. “Exploiting Mismatch Strain and the β–α Phase Transition for Microstructural Engineering in Thermoelectric Ag2Se.” ACS Energy Letters. American Chemical Society, 2026. https://doi.org/10.1021/acsenergylett.6c01499.","mla":"Liu, Yu, et al. “Exploiting Mismatch Strain and the β–α Phase Transition for Microstructural Engineering in Thermoelectric Ag2Se.” ACS Energy Letters, vol. 11, no. 8, American Chemical Society, 2026, pp. 5752–62, doi:10.1021/acsenergylett.6c01499.","short":"Y. Liu, T. Kleinhanns, M.C. Spadaro, A. Genç, S. Horta, N. Jakhar, T. Costanzo, E. Dutkiewicz, J. Arbiol, M. Hong, M. Ibáñez, ACS Energy Letters 11 (2026) 5752–5762."},"oa_version":"Published Version","OA_place":"publisher","doi":"10.1021/acsenergylett.6c01499","researchdata_availability":"no","status":"public","title":"Exploiting mismatch strain and the β–α phase transition for microstructural engineering in thermoelectric Ag2Se","date_published":"2026-08-14T00:00:00Z"},{"publication":"Journal of Applied Crystallography","quality_controlled":"1","month":"08","volume":59,"date_updated":"2026-08-20T06:14:07Z","publication_status":"published","file_date_updated":"2026-08-20T05:51:36Z","supplementarymaterial":"no","oa":1,"PlanS_conform":"1","acknowledgement":"The authors thank all respondents for their participation in the\r\nquestionnaire. We plan to make further use of the wealth of\r\nthe dataset going forward. We thank Xenocs for sharing\r\napproximate data on GISAXS equipment sales and Adrian\r\nRennie for helpful discussions. Open access funding enabled\r\nand organized by Projekt DEAL.","has_accepted_license":"1","researchdata_availability":"yes","status":"public","date_published":"2026-08-01T00:00:00Z","title":"Grazing-incidence scattering surveyed: Towards reference methods for alignment and calibration","author":[{"last_name":"Hörmann","full_name":"Hörmann, Anja F.","first_name":"Anja F."},{"orcid":"0000-0001-7597-043X","last_name":"Balazs","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","first_name":"Daniel","full_name":"Balazs, Daniel"},{"last_name":"Breßler","full_name":"Breßler, Ingo","first_name":"Ingo"},{"first_name":"Sumea","full_name":"Klokic, Sumea","last_name":"Klokic"},{"last_name":"Moradi","full_name":"Moradi, Melika","first_name":"Melika"},{"last_name":"Solano","full_name":"Solano, Eduardo","first_name":"Eduardo"},{"last_name":"Stellhorn","full_name":"Stellhorn, Annika","first_name":"Annika"},{"full_name":"Pauw, Brian R.","first_name":"Brian R.","last_name":"Pauw"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Hörmann, Anja F., Daniel Balazs, Ingo Breßler, Sumea Klokic, Melika Moradi, Eduardo Solano, Annika Stellhorn, and Brian R. Pauw. “Grazing-Incidence Scattering Surveyed: Towards Reference Methods for Alignment and Calibration.” Journal of Applied Crystallography. International Union of Crystallography, 2026. https://doi.org/10.1107/S1600576726005741.","mla":"Hörmann, Anja F., et al. “Grazing-Incidence Scattering Surveyed: Towards Reference Methods for Alignment and Calibration.” Journal of Applied Crystallography, vol. 59, no. 4, International Union of Crystallography, 2026, pp. 1247–53, doi:10.1107/S1600576726005741.","short":"A.F. Hörmann, D. Balazs, I. Breßler, S. Klokic, M. Moradi, E. Solano, A. Stellhorn, B.R. Pauw, Journal of Applied Crystallography 59 (2026) 1247–1253.","ama":"Hörmann AF, Balazs D, Breßler I, et al. Grazing-incidence scattering surveyed: Towards reference methods for alignment and calibration. Journal of Applied Crystallography. 2026;59(4):1247-1253. doi:10.1107/S1600576726005741","apa":"Hörmann, A. F., Balazs, D., Breßler, I., Klokic, S., Moradi, M., Solano, E., … Pauw, B. R. (2026). Grazing-incidence scattering surveyed: Towards reference methods for alignment and calibration. Journal of Applied Crystallography. International Union of Crystallography. https://doi.org/10.1107/S1600576726005741","ista":"Hörmann AF, Balazs D, Breßler I, Klokic S, Moradi M, Solano E, Stellhorn A, Pauw BR. 2026. Grazing-incidence scattering surveyed: Towards reference methods for alignment and calibration. Journal of Applied Crystallography. 59(4), 1247–1253.","ieee":"A. F. Hörmann et al., “Grazing-incidence scattering surveyed: Towards reference methods for alignment and calibration,” Journal of Applied Crystallography, vol. 59, no. 4. International Union of Crystallography, pp. 1247–1253, 2026."},"publisher":"International Union of Crystallography","OA_place":"publisher","oa_version":"Published Version","doi":"10.1107/S1600576726005741","department":[{"_id":"LifeSc"}],"ddc":["540"],"type":"journal_article","abstract":[{"lang":"eng","text":"Grazing-incidence small-angle scattering (GISAS) is a relatively young technique with important applications in thin-film technology and untapped potential when it comes to 2D analysis on an absolute intensity scale. Approaching standardization and reference methods early is foundational for reproducibility and comparability across laboratories and reduction of systematic error sources. It underpins trust in data obtained and accelerates innovation by ensuring that scientists work from a common methodological baseline. Accordingly, obtaining reproducible results from different GISAS instruments requires an agreement on how measurements are performed, instruments calibrated and terms defined. To pave the way for standardization and reference methods, we surveyed GISAS practitioners on what comes before an experiment: hardware, software, sample alignment and instrument calibration. Twenty-two questions were designed to elucidate the state of the art, which can be used for the development of reference methods. Our data on 27 instruments provide the basis for standardization. With very few exceptions, we found laboratories prepared to implement future reference methods, but no consensus emerges naturally for sample alignment and instrument calibration. We, that is the GISAS community, are thus in a position to embark on the journey of standardization."}],"dataavailabilitystatement":"The response data are available at https://doi.org/10.5281/\r\nzenodo.18712813. The literate programming source of this\r\nwork is available at https://doi.org/10.5281/zenodo.18713631.","article_type":"original","OA_type":"hybrid","page":"1247-1253","article_processing_charge":"Yes (via OA deal)","das_tickbox":"1","publication_identifier":{"issn":["0021-8898"],"eissn":["1600-5767"]},"issue":"4","scopus_import":"1","file":[{"file_name":"2026_JourAppliedCrystallography_Hoermann.pdf","date_updated":"2026-08-20T05:51:36Z","creator":"dernst","success":1,"file_id":"22739","relation":"main_file","file_size":6692218,"date_created":"2026-08-20T05:51:36Z","content_type":"application/pdf","checksum":"8dbad0ab078338021e2cfe722211df7b","access_level":"open_access"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"keyword":["grazing incidence","reference methods","calibration","standardization","community"],"day":"01","_id":"22715","language":[{"iso":"eng"}],"intvolume":" 59","year":"2026","date_created":"2026-08-16T22:01:43Z"},{"related_material":{"record":[{"id":"22667","relation":"dissertation_contains","status":"public"}]},"corr_author":"1","file":[{"access_level":"open_access","content_type":"application/pdf","checksum":"0d26cdb5b8d8dec3a911d8261a65cdef","file_size":14925958,"date_created":"2026-05-04T12:20:10Z","relation":"main_file","success":1,"creator":"dernst","file_id":"21795","date_updated":"2026-05-04T12:20:10Z","file_name":"2026_CellReports_Vijatovic.pdf"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"language":[{"iso":"eng"}],"_id":"21746","intvolume":" 45","day":"28","year":"2026","date_created":"2026-04-19T22:07:43Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"article_processing_charge":"Yes","project":[{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551","name":"Development and Evolution of Tetrapod Motor Circuits"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814"},{"name":"Tools for automation and feedback microscopy","grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473"},{"name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046"}],"publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"scopus_import":"1","issue":"4","publisher":"Elsevier","citation":{"chicago":"Vijatovic, David, Florina Alexandra Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell Reports. Elsevier, 2026. https://doi.org/10.1016/j.celrep.2026.117227.","mla":"Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell Reports, vol. 45, no. 4, 117227, Elsevier, 2026, doi:10.1016/j.celrep.2026.117227.","short":"D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).","ama":"Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 2026;45(4). doi:10.1016/j.celrep.2026.117227","apa":"Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2026.117227","ista":"Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227.","ieee":"D. Vijatovic et al., “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” Cell Reports, vol. 45, no. 4. Elsevier, 2026."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"orcid":"0000-0002-5494-0941","last_name":"Vijatovic","id":"cf391e77-ec3c-11ea-a124-d69323410b58","first_name":"David","full_name":"Vijatovic, David"},{"id":"2f73f876-f128-11eb-9611-b96b5a30cb0e","last_name":"Toma","full_name":"Toma, Florina Alexandra ","first_name":"Florina Alexandra "},{"last_name":"Ignatyev","first_name":"Y","full_name":"Ignatyev, Y"},{"first_name":"Zoe P","full_name":"Harrington, Zoe P","id":"a8144562-32c9-11ee-b5ce-d9800628bda2","last_name":"Harrington","orcid":"0009-0008-0158-4032"},{"full_name":"Sommer, Christoph M","first_name":"Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert"},{"full_name":"Smits, Matthijs Geert","first_name":"Matthijs Geert","id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","last_name":"Smits"},{"last_name":"Dalla Vecchia","id":"02a7a869-ff06-11ed-a87f-86649d6077e5","first_name":"Marco","full_name":"Dalla Vecchia, Marco"},{"first_name":"Alexandra J.","full_name":"Trevisan, Alexandra J.","last_name":"Trevisan"},{"last_name":"Chapman","first_name":"Phillip","full_name":"Chapman, Phillip"},{"full_name":"Julseth, Mara","first_name":"Mara","last_name":"Julseth","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1"},{"full_name":"Brenner-Morton, Susan","first_name":"Susan","last_name":"Brenner-Morton"},{"first_name":"Mariano I.","full_name":"Gabitto, Mariano I.","last_name":"Gabitto"},{"last_name":"Dasen","first_name":"Jeremy S.","full_name":"Dasen, Jeremy S."},{"last_name":"Bikoff","first_name":"Jay B.","full_name":"Bikoff, Jay B."},{"first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney"}],"doi":"10.1016/j.celrep.2026.117227","pmid":1,"OA_place":"publisher","oa_version":"Published Version","status":"public","external_id":{"pmid":["41964955 "]},"title":"Multifold increase in spinal inhibitory cell types with emergence of limb movement","article_number":"117227","date_published":"2026-04-28T00:00:00Z","OA_type":"gold","article_type":"original","abstract":[{"text":"As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution.","lang":"eng"}],"type":"journal_article","department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"ddc":["570"],"volume":45,"date_updated":"2026-08-20T14:45:18Z","publication":"Cell Reports","DOAJ_listed":"1","quality_controlled":"1","month":"04","PlanS_conform":"1","has_accepted_license":"1","acknowledgement":"We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).","publication_status":"published","oa":1,"file_date_updated":"2026-05-04T12:20:10Z"},{"language":[{"iso":"eng"}],"_id":"20870","doi":"10.1016/b978-0-443-33064-3.00016-5","oa_version":"None","day":"24","citation":{"chicago":"Stopa, Victoria, Miron Sopić, Guanliang Li, Judith Sluimer, José Basílio, Sander W. van der Laan, David P. Kreil, Yvan Devaux, and Bernhard Hochreiter. “Essentials of Transcriptomic Methods: Navigating through RNA Sequencing and Beyond.” In Transcriptomics in Atherosclerosis, edited by Yvan Devaux and Miron Sopic, 131–72. Elsevier, 2025. https://doi.org/10.1016/b978-0-443-33064-3.00016-5.","mla":"Stopa, Victoria, et al. “Essentials of Transcriptomic Methods: Navigating through RNA Sequencing and Beyond.” Transcriptomics in Atherosclerosis, edited by Yvan Devaux and Miron Sopic, Elsevier, 2025, pp. 131–72, doi:10.1016/b978-0-443-33064-3.00016-5.","short":"V. Stopa, M. Sopić, G. Li, J. Sluimer, J. Basílio, S.W. van der Laan, D.P. Kreil, Y. Devaux, B. Hochreiter, in:, Y. Devaux, M. Sopic (Eds.), Transcriptomics in Atherosclerosis, Elsevier, 2025, pp. 131–172.","ama":"Stopa V, Sopić M, Li G, et al. Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In: Devaux Y, Sopic M, eds. Transcriptomics in Atherosclerosis. Elsevier; 2025:131-172. doi:10.1016/b978-0-443-33064-3.00016-5","ieee":"V. Stopa et al., “Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond,” in Transcriptomics in Atherosclerosis, Y. Devaux and M. Sopic, Eds. Elsevier, 2025, pp. 131–172.","apa":"Stopa, V., Sopić, M., Li, G., Sluimer, J., Basílio, J., van der Laan, S. W., … Hochreiter, B. (2025). Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In Y. Devaux & M. Sopic (Eds.), Transcriptomics in Atherosclerosis (pp. 131–172). Elsevier. https://doi.org/10.1016/b978-0-443-33064-3.00016-5","ista":"Stopa V, Sopić M, Li G, Sluimer J, Basílio J, van der Laan SW, Kreil DP, Devaux Y, Hochreiter B. 2025.Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In: Transcriptomics in Atherosclerosis. , 131–172."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Elsevier","author":[{"last_name":"Stopa","first_name":"Victoria","full_name":"Stopa, Victoria"},{"full_name":"Sopić, Miron","first_name":"Miron","last_name":"Sopić"},{"full_name":"Li, Guanliang","first_name":"Guanliang","last_name":"Li"},{"first_name":"Judith","full_name":"Sluimer, Judith","last_name":"Sluimer"},{"last_name":"Basílio","first_name":"José","full_name":"Basílio, José"},{"last_name":"van der Laan","full_name":"van der Laan, Sander W.","first_name":"Sander W."},{"full_name":"Kreil, David P.","first_name":"David P.","last_name":"Kreil"},{"last_name":"Devaux","first_name":"Yvan","full_name":"Devaux, Yvan"},{"full_name":"Hochreiter, Bernhard","first_name":"Bernhard","id":"e6cab3de-17f6-11ed-9210-c1e42e045e9d","last_name":"Hochreiter"}],"date_published":"2025-10-24T00:00:00Z","title":"Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond","status":"public","OA_type":"closed access","page":"131-172","editor":[{"last_name":"Devaux","first_name":"Yvan","full_name":"Devaux, Yvan"},{"last_name":"Sopic","first_name":"Miron","full_name":"Sopic, Miron"}],"abstract":[{"text":"RNA sequencing (RNA-seq) methodologies have evolved rapidly, offering powerful tools to study gene expression, transcriptome dynamics, and molecular mechanisms in various biological contexts. However, the complexity of these approaches poses challenges in data interpretation, sensitivity, and applicability. This chapter provides a comprehensive overview of RNA-seq methodologies, highlighting their advantages, limitations, and applications, particularly in cardiovascular research. Bulk RNA sequencing enables high-throughput gene expression profiling but lacks the resolution to capture cellular heterogeneity and spatial context. Direct RNA sequencing preserves native RNA modifications, offering insights into post-transcriptional regulation, though it remains technically challenging. Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) bridge these gaps by resolving transcriptomic complexity at the cellular level and within tissue architecture, providing crucial insights into disease mechanisms such as atherosclerosis. By summarizing the strengths and limitations of these methodologies, this chapter aims to guide researchers in selecting the most suitable transcriptomic approach for their studies, ultimately advancing precision medicine and biomarker discovery in cardiovascular disease.","lang":"eng"}],"type":"book_chapter","year":"2025","department":[{"_id":"Bio"}],"date_created":"2025-12-29T12:16:22Z","date_updated":"2026-01-05T11:49:54Z","article_processing_charge":"No","month":"10","quality_controlled":"1","publication":"Transcriptomics in Atherosclerosis","scopus_import":"1","publication_identifier":{"isbn":["9780443330643"]},"publication_status":"published"},{"acknowledgement":"We would like to thank the members of the Sweeney Lab for discussion and support; Andrey\r\nBydanov for technical assistance with single-cell sequencing processing; and Jay Bikoff,\r\nNikos Konstantinides, Maria Tosches, and Graziana Gatto for comments on the manuscript. \r\nThis research was supported by: Horizon Europe ERC Starting Grant 101041551 (L.B.S,\r\nY.I., S.P.); Special Research Program (SFB) of the Austrian Science Fund (FWF) F7814-B\r\n(L.B.S., S.P., E.M.T); Austrian Science Fund (FWF) 10.55776/COE16 (L.B.S., Y.I., E.M.T.);\r\nAustrian Academy of Sciences DOC Fellowship 27229 (S.P.); ERC Advanced Grant 742046\r\n(E.M.T.); NIH award R24 OD031956 (L.P.); and in part by the Intramural Research\r\nProgram of the National Institutes of Health (NIH) through 1ZIA NS003153 to A.J.L.\r\nThe contributions of the NIH author are considered Works of the United States\r\nGovernment. The findings and conclusions presented in this paper are those of\r\nthe authors and do not necessarily reflect the views of the NIH or the U.S. Department\r\nof Health and Human Services. ","publication_status":"submitted","oa":1,"article_processing_charge":"No","date_updated":"2026-05-27T07:25:41Z","publication":"bioRxiv","month":"10","project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"},{"grant_number":"27229","_id":"907b765e-16d5-11f0-9cad-fef108a945b1","name":"A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb transition of Xenopus metamorphosis"}],"main_file_link":[{"url":"https://doi.org/10.1101/2025.10.09.680955","open_access":"1"}],"OA_type":"green","type":"preprint","abstract":[{"lang":"eng","text":"Vertebrates display remarkable diversity of sensorimotor behaviors, each adapted to distinct ecological and survival demands. This diversity raises fundamental questions about the evolutionary origin of motor control: do conserved spinal circuits underlie these behaviors, and how have they diverged across species. Recent studies detail spinal cell-type architecture in mammals but comparable, high-resolution atlases of the non-mammalian spinal cord are lacking. Here, we compare spinal cord cell types between fish, frogs, mice and humans, spanning ∼450 million years of evolution. Across species, we define highly conserved programs of cell type specification that segregate spinal neurons into nearly identical cardinal classes during development. This contrasts with adult stages, when spinal cell-type composition selectively diverges for excitatory neuron subpopulations. Using spatial transcriptomics, we localize this species divergence to the superficial, dorsal spinal cord, where variant neuropeptide expression defines mammalian-specific cell types. The most dorsal spinal cord thus emerges as a recently evolved hub for sensory integration in mammals, a neospinal cord analogous to the neocortex."}],"date_created":"2026-05-27T06:54:04Z","department":[{"_id":"LoSw"},{"_id":"ScienComp"}],"year":"2025","corr_author":"1","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","citation":{"apa":"Ignatyev, Y., Papadopoulos, S., Soretić, M., Yeung, J., Lin, T.-Y., Tanaka, E. M., … Sweeney, L. B. (n.d.). Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv. https://doi.org/10.1101/2025.10.09.680955","ista":"Ignatyev Y, Papadopoulos S, Soretić M, Yeung J, Lin T-Y, Tanaka EM, Peshkin L, Levine AJ, Gabitto MI, Sweeney LB. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv, 10.1101/2025.10.09.680955.","ieee":"Y. Ignatyev et al., “Innovations in spinal cord cell type heterogeneity across vertebrate evolution,” bioRxiv. .","ama":"Ignatyev Y, Papadopoulos S, Soretić M, et al. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv. doi:10.1101/2025.10.09.680955","mla":"Ignatyev, Yuri, et al. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” BioRxiv, doi:10.1101/2025.10.09.680955.","short":"Y. Ignatyev, S. Papadopoulos, M. Soretić, J. Yeung, T.-Y. Lin, E.M. Tanaka, L. Peshkin, A.J. Levine, M.I. Gabitto, L.B. Sweeney, BioRxiv (n.d.).","chicago":"Ignatyev, Yuri, Stavros Papadopoulos, Mateja Soretić, Jake Yeung, Tzi-Yang Lin, Elly M Tanaka, Leonid Peshkin, Ariel J Levine, Mariano I Gabitto, and Lora B. Sweeney. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” BioRxiv, n.d. https://doi.org/10.1101/2025.10.09.680955."},"author":[{"first_name":"Yuri","full_name":"Ignatyev, Yuri","last_name":"Ignatyev"},{"full_name":"Papadopoulos, Stavros","first_name":"Stavros","id":"40606b92-f128-11eb-9611-bf66a98cfa5c","last_name":"Papadopoulos"},{"last_name":"Soretić","first_name":"Mateja","full_name":"Soretić, Mateja"},{"full_name":"Yeung, Jake","first_name":"Jake","orcid":"0000-0003-1732-1559","id":"123012b2-db30-11eb-b4d8-a35840c0551b","last_name":"Yeung"},{"first_name":"Tzi-Yang","full_name":"Lin, Tzi-Yang","last_name":"Lin"},{"last_name":"Tanaka","first_name":"Elly M","full_name":"Tanaka, Elly M"},{"first_name":"Leonid","full_name":"Peshkin, Leonid","last_name":"Peshkin"},{"last_name":"Levine","first_name":"Ariel J","full_name":"Levine, Ariel J"},{"last_name":"Gabitto","full_name":"Gabitto, Mariano I","first_name":"Mariano I"},{"first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney"}],"tmp":{"short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"_id":"21920","doi":"10.1101/2025.10.09.680955","language":[{"iso":"eng"}],"oa_version":"Preprint","day":"11","OA_place":"repository","status":"public","title":"Innovations in spinal cord cell type heterogeneity across vertebrate evolution","date_published":"2025-10-11T00:00:00Z"}]