[{"publication_status":"published","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"EvBe"},{"_id":"EM-Fac"}],"volume":609,"language":[{"iso":"eng"}],"page":"575-581","month":"09","article_processing_charge":"No","date_created":"2023-01-16T10:04:48Z","quality_controlled":"1","related_material":{"record":[{"id":"19395","relation":"dissertation_contains","status":"public"},{"status":"public","id":"23145","relation":"dissertation_contains"},{"id":"20364","relation":"dissertation_contains","status":"public"}]},"project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"name":"RNA-directed DNA methylation in plant development","_id":"262EF96E-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"P29988"}],"scopus_import":"1","corr_author":"1","author":[{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"},{"first_name":"Michelle C","orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Gelová","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","full_name":"Gelová, Zuzana","first_name":"Zuzana","orcid":"0000-0003-4783-1752"},{"full_name":"Johnson, Alexander J","last_name":"Johnson","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2739-8843","first_name":"Alexander J"},{"first_name":"Ewa","last_name":"Mazur","full_name":"Mazur, Ewa"},{"last_name":"Monzer","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","full_name":"Monzer, Aline","first_name":"Aline"},{"full_name":"Rodriguez Solovey, Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87","last_name":"Rodriguez Solovey","first_name":"Lesia","orcid":"0000-0002-7244-7237"},{"full_name":"Roosjen, Mark","last_name":"Roosjen","first_name":"Mark"},{"id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten","full_name":"Verstraeten, Inge","first_name":"Inge","orcid":"0000-0001-7241-2328"},{"first_name":"Branka D.","full_name":"Živanović, Branka D.","last_name":"Živanović"},{"full_name":"Zou, Minxia","last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","first_name":"Minxia"},{"full_name":"Fiedler, Lukas","last_name":"Fiedler","id":"7c417475-8972-11ed-ae7b-8b674ca26986","first_name":"Lukas"},{"first_name":"Caterina","full_name":"Giannini, Caterina","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","last_name":"Giannini"},{"first_name":"Peter","last_name":"Grones","full_name":"Grones, Peter"},{"id":"45A71A74-F248-11E8-B48F-1D18A9856A87","last_name":"Hrtyan","full_name":"Hrtyan, Mónika","first_name":"Mónika"},{"first_name":"Walter","orcid":"0000-0001-9735-5315","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","full_name":"Kaufmann, Walter"},{"last_name":"Kuhn","full_name":"Kuhn, Andre","first_name":"Andre"},{"first_name":"Madhumitha","orcid":"0000-0002-8600-0671","last_name":"Narasimhan","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","full_name":"Narasimhan, Madhumitha"},{"full_name":"Randuch, Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","first_name":"Marek"},{"first_name":"Nikola","last_name":"Rýdza","full_name":"Rýdza, Nikola"},{"full_name":"Takahashi, Koji","last_name":"Takahashi","first_name":"Koji"},{"id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285","first_name":"Shutang"},{"full_name":"Teplova, Anastasiia","last_name":"Teplova","id":"e3736151-106c-11ec-b916-c2558e2762c6","first_name":"Anastasiia"},{"full_name":"Kinoshita, Toshinori","last_name":"Kinoshita","first_name":"Toshinori"},{"last_name":"Weijers","full_name":"Weijers, Dolf","first_name":"Dolf"},{"first_name":"Hana","last_name":"Rakusová","full_name":"Rakusová, Hana"}],"publication":"Nature","type":"journal_article","doi":"10.1038/s41586-022-05187-x","title":"ABP1–TMK auxin perception for global phosphorylation and auxin canalization","oa":1,"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"pmid":1,"oa_version":"Submitted Version","status":"public","_id":"12291","publisher":"Springer Nature","acknowledgement":"We acknowledge K. Kubiasová for excellent technical assistance, J. Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI; and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM (MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F. is grateful to R. Napier for many insightful suggestions and support. We thank all past and present members of the Friml group for their support and for other contributions to this effort to clarify the controversial role of ABP1 over the past seven years. The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.); the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001 to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053 to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and 20H05910).","year":"2022","citation":{"ama":"Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. 2022;609(7927):575-581. doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>","ista":"Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K, Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581.","ieee":"J. Friml <i>et al.</i>, “ABP1–TMK auxin perception for global phosphorylation and auxin canalization,” <i>Nature</i>, vol. 609, no. 7927. Springer Nature, pp. 575–581, 2022.","apa":"Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A., … Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>","chicago":"Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>.","mla":"Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>, vol. 609, no. 7927, Springer Nature, 2022, pp. 575–81, doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>.","short":"J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini, P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza, K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature 609 (2022) 575–581."},"isi":1,"day":"15","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"intvolume":"       609","file":[{"access_level":"open_access","checksum":"a6055c606aefb900bf62ae3e7d15f921","relation":"main_file","creator":"amally","content_type":"application/pdf","date_updated":"2023-11-02T17:12:37Z","success":1,"file_id":"14483","file_size":79774945,"date_created":"2023-11-02T17:12:37Z","file_name":"Friml Nature 2022_merged.pdf"}],"issue":"7927","external_id":{"isi":["000851357500002"],"pmid":["36071161"]},"date_updated":"2026-10-26T23:31:08Z","abstract":[{"lang":"eng","text":"The phytohormone auxin triggers transcriptional reprogramming through a well-characterized perception machinery in the nucleus. By contrast, mechanisms that underlie fast effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required for the auxin-induced ultrafast global phospho-response and for downstream processes that include the activation of H+-ATPase and accelerated cytoplasmic streaming. abp1 and tmk mutants cannot establish auxin-transporting channels and show defective auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that lacks the capacity to bind auxin is unable to complement these defects in abp1 mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface signalling, which mediates the global phospho-response and auxin canalization."}],"ec_funded":1,"file_date_updated":"2023-11-02T17:12:37Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1038/s41586-022-05187-x","article_type":"original","date_published":"2022-09-15T00:00:00Z","has_accepted_license":"1","ddc":["580"]},{"status":"public","_id":"10836","oa_version":"Published Version","day":"01","isi":1,"citation":{"mla":"Pranger, Christina L., et al. “PIPE‐cloned Human IgE and IgG4 Antibodies: New Tools for Investigating Cow’s Milk Allergy and Tolerance.” <i>Allergy</i>, vol. 76, no. 5, Wiley, 2021, pp. 1553–56, doi:<a href=\"https://doi.org/10.1111/all.14604\">10.1111/all.14604</a>.","short":"C.L. Pranger, J. Singer, V.K. Köhler, I. Pali‐Schöll, A. Fiocchi, S.N. Karagiannis, O. Zenarruzabeitia, F. Borrego, E. Jensen‐Jarolim, Allergy 76 (2021) 1553–1556.","chicago":"Pranger, Christina L., Judit Singer, Verena K. Köhler, Isabella Pali‐Schöll, Alessandro Fiocchi, Sophia N. Karagiannis, Olatz Zenarruzabeitia, Francisco Borrego, and Erika Jensen‐Jarolim. “PIPE‐cloned Human IgE and IgG4 Antibodies: New Tools for Investigating Cow’s Milk Allergy and Tolerance.” <i>Allergy</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/all.14604\">https://doi.org/10.1111/all.14604</a>.","ieee":"C. L. Pranger <i>et al.</i>, “PIPE‐cloned human IgE and IgG4 antibodies: New tools for investigating cow’s milk allergy and tolerance,” <i>Allergy</i>, vol. 76, no. 5. Wiley, pp. 1553–1556, 2021.","apa":"Pranger, C. L., Singer, J., Köhler, V. K., Pali‐Schöll, I., Fiocchi, A., Karagiannis, S. N., … Jensen‐Jarolim, E. (2021). PIPE‐cloned human IgE and IgG4 antibodies: New tools for investigating cow’s milk allergy and tolerance. <i>Allergy</i>. Wiley. <a href=\"https://doi.org/10.1111/all.14604\">https://doi.org/10.1111/all.14604</a>","ama":"Pranger CL, Singer J, Köhler VK, et al. PIPE‐cloned human IgE and IgG4 antibodies: New tools for investigating cow’s milk allergy and tolerance. <i>Allergy</i>. 2021;76(5):1553-1556. doi:<a href=\"https://doi.org/10.1111/all.14604\">10.1111/all.14604</a>","ista":"Pranger CL, Singer J, Köhler VK, Pali‐Schöll I, Fiocchi A, Karagiannis SN, Zenarruzabeitia O, Borrego F, Jensen‐Jarolim E. 2021. PIPE‐cloned human IgE and IgG4 antibodies: New tools for investigating cow’s milk allergy and tolerance. Allergy. 76(5), 1553–1556."},"acknowledgement":"This  work  was  supported  by  the  Austrian  Science  Fund  (FWF)  grants  MCCA  W1248-B30  and  SFB  F4606-B28  to  EJJ.  CP  received  a  short-term research fellowship of the European Federation of Immunological Societies  (EFIS-IL)  for  a  research  visit  at  Biocruces  Bizkaia  Health  Research  Institute,  Barakaldo,  Spain.  VKK  received  an  EFIS-IL  short-term  research  fellowship  for  a  research  visit  at  King’s  College  London.  The research was funded by the National Institute for Health Research (NIHR) Biomedical Research Centre (BRC) based at Guy's and St Thomas' NHS Foundation Trust and King's College London (IS-BRC-1215-20006) (SNK).  The  authors  acknowledge  support  by  the  Medical  Research  Council (MR/L023091/1) (SNK); Breast Cancer Now (147; KCL-BCN-Q3)(SNK); Cancer Research UK (C30122/A11527; C30122/A15774) (SNK); Cancer  Research  UK  King's  Health  Partners  Centre  at  King's  College  London   (C604/A25135)   (SNK);   CRUK/NIHR   in   England/DoH   for   Scotland,  Wales  and  Northern  Ireland  Experimental  Cancer  Medicine  Centre  (C10355/A15587)  (SNK).  The  views  expressed  are  those  of  the  author(s)  and  not  necessarily  those  of  the  NHS,  the  NIHR  or  the  Department  of  Health.  Additionally,  this  work  was  funded  by  Instituto  de  Salud  Carlos  III  through  the  project  \"PI16/01223\"  (Co-funded  by  European Regional Development Fund; “A way to make Europe”) to FB and  by  the  Department  of  Health,  Basque  Government  through  the  project “2019111031” to OZ. OZ is recipient of a Sara Borrell 2017 post-doctoral contract “CD17/00128” funded by Instituto de Salud Carlos III (Co-funded by European Social Fund; “Investing in your future”).","year":"2021","publisher":"Wiley","external_id":{"isi":["000577708800001"],"pmid":["32990982"]},"issue":"5","file":[{"content_type":"application/pdf","date_updated":"2022-03-08T11:23:16Z","creator":"dernst","relation":"main_file","checksum":"9526f9554112fc027c9f7fa540c488cd","access_level":"open_access","file_name":"2021_Allergy_Pranger.pdf","file_size":626081,"date_created":"2022-03-08T11:23:16Z","file_id":"10837","success":1}],"intvolume":"        76","date_updated":"2023-09-05T15:58:53Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file_date_updated":"2022-03-08T11:23:16Z","ddc":["570"],"date_published":"2021-05-01T00:00:00Z","has_accepted_license":"1","fulldoi":"https://doi.org/10.1111/all.14604","article_type":"letter_note","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"keyword":["Immunology","Immunology and Allergy"],"page":"1553-1556","department":[{"_id":"Bio"}],"volume":76,"language":[{"iso":"eng"}],"publication_status":"published","month":"05","license":"https://creativecommons.org/licenses/by/4.0/","date_created":"2022-03-08T11:19:05Z","article_processing_charge":"No","quality_controlled":"1","scopus_import":"1","publication":"Allergy","author":[{"first_name":"Christina L.","full_name":"Pranger, Christina L.","last_name":"Pranger"},{"full_name":"Fazekas-Singer, Judit","id":"36432834-F248-11E8-B48F-1D18A9856A87","last_name":"Fazekas-Singer","orcid":"0000-0002-8777-3502","first_name":"Judit"},{"last_name":"Köhler","full_name":"Köhler, Verena K.","first_name":"Verena K."},{"last_name":"Pali‐Schöll","full_name":"Pali‐Schöll, Isabella","first_name":"Isabella"},{"first_name":"Alessandro","full_name":"Fiocchi, Alessandro","last_name":"Fiocchi"},{"full_name":"Karagiannis, Sophia N.","last_name":"Karagiannis","first_name":"Sophia N."},{"last_name":"Zenarruzabeitia","full_name":"Zenarruzabeitia, Olatz","first_name":"Olatz"},{"last_name":"Borrego","full_name":"Borrego, Francisco","first_name":"Francisco"},{"last_name":"Jensen‐Jarolim","full_name":"Jensen‐Jarolim, Erika","first_name":"Erika"}],"pmid":1,"publication_identifier":{"eissn":["1398-9995"],"issn":["0105-4538"]},"oa":1,"title":"PIPE‐cloned human IgE and IgG4 antibodies: New tools for investigating cow's milk allergy and tolerance","doi":"10.1111/all.14604","type":"journal_article"},{"abstract":[{"text":"Significant advances in the synthesis and processing of colloidal nanocrystals have given scientists and engineers access to a vast library of building blocks with precisely defined size, shape, and composition. These materials have inspired exciting prospects to enable bottom-up fabrication of programmable materials with properties by design. Successfully assembling and connecting the building blocks into superstructures in which constituent nanocrystals can purposefully interact requires robust understanding of and control over a complex interplay of dynamic physicochemical processes. Fluid interfaces provide an advantageous experimental workbench to both probe and control these processes. Despite the ostensible simplicity of fabricating nanocrystal assemblies at a fluid interface, sensitivity to processing conditions and limited reproducibility have underscored the complexity of this process. In situ studies have provided mechanistic insights into the competing dynamics of key subprocesses including solvent spreading and evaporation, superlattice formation, ligand detachment kinetics, and nanocrystal attachment. Understanding how these subprocesses influence the complex choreography of self-assembly, structure transformation, and oriented attachment processes presents a rich research challenge. In this context, we present a detailed methodology for self-assembly and attachment of lead chalcogenide nanocrystals at a liquid–gas interface as a model system for the fabrication of mono- and multilayer cubic connected superlattices. We discuss key experimental parameters such as the characteristics of the building blocks and processing conditions and detailed steps from colloidal nanocrystal injection to superlattice transfer. We hope that this Methods/Protocols paper will provide guidance for future advances in the exciting path toward bringing the prospect of nanocrystal-based programmable materials to fruition.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2024-04-03T13:50:53Z","keyword":["Materials Chemistry","General Chemical Engineering","General Chemistry"],"article_type":"original","fulldoi":"https://doi.org/10.1021/acs.chemmater.1c02910","date_published":"2021-12-16T00:00:00Z","publisher":"American Chemical Society","year":"2021","citation":{"ista":"Cimada daSilva J, Balazs D, Dunbar TA, Hanrath T. 2021. Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface. Chemistry of Materials. 33(24), 9457–9472.","ama":"Cimada daSilva J, Balazs D, Dunbar TA, Hanrath T. Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface. <i>Chemistry of Materials</i>. 2021;33(24):9457-9472. doi:<a href=\"https://doi.org/10.1021/acs.chemmater.1c02910\">10.1021/acs.chemmater.1c02910</a>","apa":"Cimada daSilva, J., Balazs, D., Dunbar, T. A., &#38; Hanrath, T. (2021). Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface. <i>Chemistry of Materials</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.chemmater.1c02910\">https://doi.org/10.1021/acs.chemmater.1c02910</a>","ieee":"J. Cimada daSilva, D. Balazs, T. A. Dunbar, and T. Hanrath, “Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface,” <i>Chemistry of Materials</i>, vol. 33, no. 24. American Chemical Society, pp. 9457–9472, 2021.","chicago":"Cimada daSilva, Jessica, Daniel Balazs, Tyler A. Dunbar, and Tobias Hanrath. “Fundamental Processes and Practical Considerations of Lead Chalcogenide Mesocrystals Formed via Self-Assembly and Directed Attachment of Nanocrystals at a Fluid Interface.” <i>Chemistry of Materials</i>. American Chemical Society, 2021. <a href=\"https://doi.org/10.1021/acs.chemmater.1c02910\">https://doi.org/10.1021/acs.chemmater.1c02910</a>.","mla":"Cimada daSilva, Jessica, et al. “Fundamental Processes and Practical Considerations of Lead Chalcogenide Mesocrystals Formed via Self-Assembly and Directed Attachment of Nanocrystals at a Fluid Interface.” <i>Chemistry of Materials</i>, vol. 33, no. 24, American Chemical Society, 2021, pp. 9457–72, doi:<a href=\"https://doi.org/10.1021/acs.chemmater.1c02910\">10.1021/acs.chemmater.1c02910</a>.","short":"J. Cimada daSilva, D. Balazs, T.A. Dunbar, T. Hanrath, Chemistry of Materials 33 (2021) 9457–9472."},"day":"16","oa_version":"Submitted Version","_id":"15260","status":"public","intvolume":"        33","issue":"24","author":[{"first_name":"Jessica","last_name":"Cimada daSilva","full_name":"Cimada daSilva, Jessica"},{"first_name":"Daniel","orcid":"0000-0001-7597-043X","id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","last_name":"Balazs","full_name":"Balazs, Daniel"},{"full_name":"Dunbar, Tyler A.","last_name":"Dunbar","first_name":"Tyler A."},{"last_name":"Hanrath","full_name":"Hanrath, Tobias","first_name":"Tobias"}],"publication":"Chemistry of Materials","scopus_import":"1","type":"journal_article","main_file_link":[{"url":"https://www.osti.gov/servlets/purl/1836502","open_access":"1"}],"doi":"10.1021/acs.chemmater.1c02910","oa":1,"title":"Fundamental processes and practical considerations of lead chalcogenide mesocrystals formed via self-assembly and directed attachment of nanocrystals at a fluid interface","publication_identifier":{"eissn":["1520-5002"],"issn":["0897-4756"]},"month":"12","publication_status":"published","department":[{"_id":"LifeSc"}],"language":[{"iso":"eng"}],"volume":33,"page":"9457-9472","quality_controlled":"1","article_processing_charge":"No","date_created":"2024-04-03T07:23:30Z"},{"doi":"10.3390/hearts2030031","type":"journal_article","publication_identifier":{"issn":["2673-3846"]},"oa":1,"title":"The history and challenges of SCP-ECG: The standard communication protocol for computer-assisted electrocardiography","author":[{"first_name":"Paul","full_name":"Rubel, Paul","last_name":"Rubel"},{"first_name":"Jocelyne","last_name":"Fayn","full_name":"Fayn, Jocelyne"},{"last_name":"Macfarlane","full_name":"Macfarlane, Peter W.","first_name":"Peter W."},{"first_name":"Danilo","last_name":"Pani","full_name":"Pani, Danilo"},{"full_name":"Schlögl, Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl","orcid":"0000-0002-5621-8100","first_name":"Alois"},{"last_name":"Värri","full_name":"Värri, Alpo","first_name":"Alpo"}],"publication":"Hearts","quality_controlled":"1","date_created":"2024-04-03T09:03:31Z","article_processing_charge":"Yes","month":"08","language":[{"iso":"eng"}],"volume":2,"department":[{"_id":"ScienComp"}],"publication_status":"published","page":"384-409","article_type":"review","fulldoi":"https://doi.org/10.3390/hearts2030031","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"keyword":["General Medicine"],"ddc":["610"],"has_accepted_license":"1","date_published":"2021-08-24T00:00:00Z","file_date_updated":"2024-04-09T06:49:47Z","abstract":[{"text":"Ever since the first publication of the standard communication protocol for computer-assisted electrocardiography (SCP-ECG), prENV 1064, in 1993, by the European Committee for Standardization (CEN), SCP-ECG has become a leading example in health informatics, enabling open, secure, and well-documented digital data exchange at a low cost, for quick and efficient cardiovascular disease detection and management. Based on the experiences gained, since the 1970s, in computerized electrocardiology, and on the results achieved by the pioneering, international cooperative research on common standards for quantitative electrocardiography (CSE), SCP-ECG was designed, from the beginning, to empower personalized medicine, thanks to serial ECG analysis. The fundamental concept behind SCP-ECG is to convey the necessary information for ECG re-analysis, serial comparison, and interpretation, and to structure the ECG data and metadata in sections that are mostly optional in order to fit all use cases. SCP-ECG is open to the storage of the ECG signal and ECG measurement data, whatever the ECG recording modality or computation method, and can store the over-reading trails and ECG annotations, as well as any computerized or medical interpretation reports. Only the encoding syntax and the semantics of the ECG descriptors and of the diagnosis codes are standardized. We present all of the landmarks in the development and publication of SCP-ECG, from the early 1990s to the 2009 International Organization for Standardization (ISO) SCP-ECG standards, including the latest version published by CEN in 2020, which now encompasses rest and stress ECGs, Holter recordings, and protocol-based trials.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2024-04-09T06:51:50Z","file":[{"file_id":"15302","success":1,"file_name":"2021_Hearts_Rubel.pdf","date_created":"2024-04-09T06:49:47Z","file_size":3539897,"relation":"main_file","checksum":"f67142b1e1e8ca5cd7a6a6798f46375e","access_level":"open_access","date_updated":"2024-04-09T06:49:47Z","content_type":"application/pdf","creator":"dernst"}],"intvolume":"         2","issue":"3","citation":{"ama":"Rubel P, Fayn J, Macfarlane PW, Pani D, Schlögl A, Värri A. The history and challenges of SCP-ECG: The standard communication protocol for computer-assisted electrocardiography. <i>Hearts</i>. 2021;2(3):384-409. doi:<a href=\"https://doi.org/10.3390/hearts2030031\">10.3390/hearts2030031</a>","ista":"Rubel P, Fayn J, Macfarlane PW, Pani D, Schlögl A, Värri A. 2021. The history and challenges of SCP-ECG: The standard communication protocol for computer-assisted electrocardiography. Hearts. 2(3), 384–409.","ieee":"P. Rubel, J. Fayn, P. W. Macfarlane, D. Pani, A. Schlögl, and A. Värri, “The history and challenges of SCP-ECG: The standard communication protocol for computer-assisted electrocardiography,” <i>Hearts</i>, vol. 2, no. 3. MDPI, pp. 384–409, 2021.","apa":"Rubel, P., Fayn, J., Macfarlane, P. W., Pani, D., Schlögl, A., &#38; Värri, A. (2021). The history and challenges of SCP-ECG: The standard communication protocol for computer-assisted electrocardiography. <i>Hearts</i>. MDPI. <a href=\"https://doi.org/10.3390/hearts2030031\">https://doi.org/10.3390/hearts2030031</a>","chicago":"Rubel, Paul, Jocelyne Fayn, Peter W. Macfarlane, Danilo Pani, Alois Schlögl, and Alpo Värri. “The History and Challenges of SCP-ECG: The Standard Communication Protocol for Computer-Assisted Electrocardiography.” <i>Hearts</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/hearts2030031\">https://doi.org/10.3390/hearts2030031</a>.","mla":"Rubel, Paul, et al. “The History and Challenges of SCP-ECG: The Standard Communication Protocol for Computer-Assisted Electrocardiography.” <i>Hearts</i>, vol. 2, no. 3, MDPI, 2021, pp. 384–409, doi:<a href=\"https://doi.org/10.3390/hearts2030031\">10.3390/hearts2030031</a>.","short":"P. Rubel, J. Fayn, P.W. Macfarlane, D. Pani, A. Schlögl, A. Värri, Hearts 2 (2021) 384–409."},"year":"2021","acknowledgement":"This research received no external funding. The authors thank the large number of researchers, engineers, cardiologists, and clinicians from academia, industry, and normalization organizations who contributed to the development and testing of the SCP-ECG standards.","publisher":"MDPI","day":"24","oa_version":"Published Version","_id":"15285","status":"public"},{"month":"09","publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"MaDe"},{"_id":"LifeSc"}],"volume":297,"quality_controlled":"1","project":[{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411"}],"article_processing_charge":"Yes","date_created":"2021-10-10T22:01:23Z","author":[{"first_name":"Murat","orcid":"0000-0001-8945-6992","id":"C407B586-6052-11E9-B3AE-7006E6697425","last_name":"Artan","full_name":"Artan, Murat"},{"full_name":"Barratt, Stephen","last_name":"Barratt","id":"57740d2b-2a88-11ec-97cf-d9e6d1b39677","first_name":"Stephen"},{"first_name":"Sean M.","full_name":"Flynn, Sean M.","last_name":"Flynn"},{"first_name":"Farida","last_name":"Begum","full_name":"Begum, Farida"},{"first_name":"Mark","full_name":"Skehel, Mark","last_name":"Skehel"},{"first_name":"Armel","full_name":"Nicolas, Armel","id":"2A103192-F248-11E8-B48F-1D18A9856A87","last_name":"Nicolas"},{"id":"4E3FF80E-F248-11E8-B48F-1D18A9856A87","last_name":"De Bono","full_name":"De Bono, Mario","first_name":"Mario","orcid":"0000-0001-8347-0443"}],"publication":"Journal of Biological Chemistry","scopus_import":"1","article_number":"101094","type":"journal_article","doi":"10.1016/J.JBC.2021.101094","oa":1,"title":"Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling","publication_identifier":{"eissn":["1083-351X"],"issn":["0021-9258"]},"publisher":"Elsevier","year":"2021","citation":{"ieee":"M. Artan <i>et al.</i>, “Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling,” <i>Journal of Biological Chemistry</i>, vol. 297, no. 3. Elsevier, 2021.","apa":"Artan, M., Barratt, S., Flynn, S. M., Begum, F., Skehel, M., Nicolas, A., &#38; de Bono, M. (2021). Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. <i>Journal of Biological Chemistry</i>. Elsevier. <a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">https://doi.org/10.1016/J.JBC.2021.101094</a>","ama":"Artan M, Barratt S, Flynn SM, et al. Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. <i>Journal of Biological Chemistry</i>. 2021;297(3). doi:<a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">10.1016/J.JBC.2021.101094</a>","ista":"Artan M, Barratt S, Flynn SM, Begum F, Skehel M, Nicolas A, de Bono M. 2021. Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. Journal of Biological Chemistry. 297(3), 101094.","short":"M. Artan, S. Barratt, S.M. Flynn, F. Begum, M. Skehel, A. Nicolas, M. de Bono, Journal of Biological Chemistry 297 (2021).","mla":"Artan, Murat, et al. “Interactome Analysis of Caenorhabditis Elegans Synapses by TurboID-Based Proximity Labeling.” <i>Journal of Biological Chemistry</i>, vol. 297, no. 3, 101094, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">10.1016/J.JBC.2021.101094</a>.","chicago":"Artan, Murat, Stephen Barratt, Sean M. Flynn, Farida Begum, Mark Skehel, Armel Nicolas, and Mario de Bono. “Interactome Analysis of Caenorhabditis Elegans Synapses by TurboID-Based Proximity Labeling.” <i>Journal of Biological Chemistry</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/J.JBC.2021.101094\">https://doi.org/10.1016/J.JBC.2021.101094</a>."},"acknowledgement":"We thank de Bono lab members for helpful comments on the manuscript, IST Austria and University of Vienna Mass Spec Facilities for invaluable discussions and comments for the optimization of mass spec analyses of worm samples. The biotin auxotropic E. coli strain MG1655bioB:kan was gift from John Cronan (University of Illinois) and was kindly sent to us by Jessica Feldman and Ariana Sanchez (Stanford University). dg398 pEntryslot2_mNeongreen::3XFLAG::stop and dg397 pEntryslot3_mNeongreen::3XFLAG::stop::unc-54 3′UTR entry vector were kindly shared by Dr Dominique Glauser (University of Fribourg). Codon-optimized mScarlet vector was a generous gift from Dr Manuel Zimmer (University of Vienna).","isi":1,"day":"01","oa_version":"Published Version","_id":"10117","status":"public","intvolume":"       297","file":[{"access_level":"open_access","checksum":"19e39d36c5b9387c6dc0e89c9ae856ab","relation":"main_file","content_type":"application/pdf","date_updated":"2021-10-11T12:20:58Z","creator":"cchlebak","success":1,"file_id":"10121","file_name":"2021_JBC_Artan.pdf","date_created":"2021-10-11T12:20:58Z","file_size":1680010}],"issue":"3","external_id":{"isi":["000706409200006"]},"ec_funded":1,"file_date_updated":"2021-10-11T12:20:58Z","abstract":[{"text":"Proximity labeling provides a powerful in vivo tool to characterize the proteome of subcellular structures and the interactome of specific proteins. The nematode Caenorhabditis elegans is one of the most intensely studied organisms in biology, offering many advantages for biochemistry. Using the highly active biotin ligase TurboID, we optimize here a proximity labeling protocol for C. elegans. An advantage of TurboID is that biotin's high affinity for streptavidin means biotin-labeled proteins can be affinity-purified under harsh denaturing conditions. By combining extensive sonication with aggressive denaturation using SDS and urea, we achieved near-complete solubilization of worm proteins. We then used this protocol to characterize the proteomes of the worm gut, muscle, skin, and nervous system. Neurons are among the smallest C. elegans cells. To probe the method's sensitivity, we expressed TurboID exclusively in the two AFD neurons and showed that the protocol could identify known and previously unknown proteins expressed selectively in AFD. The active zones of synapses are composed of a protein matrix that is difficult to solubilize and purify. To test if our protocol could solubilize active zone proteins, we knocked TurboID into the endogenous elks-1 gene, which encodes a presynaptic active zone protein. We identified many known ELKS-1-interacting active zone proteins, as well as previously uncharacterized synaptic proteins. Versatile vectors and the inherent advantages of using C. elegans, including fast growth and the ability to rapidly make and functionally test knock-ins, make proximity labeling a valuable addition to the armory of this model organism.","lang":"eng"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2025-04-14T07:43:46Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","fulldoi":"https://doi.org/10.1016/J.JBC.2021.101094","date_published":"2021-09-01T00:00:00Z","has_accepted_license":"1","ddc":["612"]},{"has_accepted_license":"1","date_published":"2021-10-08T00:00:00Z","ddc":["530"],"tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png"},"fulldoi":"https://doi.org/10.1126/sciadv.abj0127","article_type":"original","date_updated":"2026-04-02T13:15:46Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","abstract":[{"text":"Phonon polaritons (PhPs)—light coupled to lattice vibrations—with in-plane hyperbolic dispersion exhibit ray-like propagation with large wave vectors and enhanced density of optical states along certain directions on a surface. As such, they have raised a surge of interest, promising unprecedented manipulation of infrared light at the nanoscale in a planar circuitry. Here, we demonstrate focusing of in-plane hyperbolic PhPs propagating along thin slabs of α-MoO3. To that end, we developed metallic nanoantennas of convex geometries for both efficient launching and focusing of the polaritons. The foci obtained exhibit enhanced near-field confinement and absorption compared to foci produced by in-plane isotropic PhPs. Foci sizes as small as λp/4.5 = λ0/50 were achieved (λp is the polariton wavelength and λ0 is the photon wavelength). Focusing of in-plane hyperbolic polaritons introduces a first and most basic building block developing planar polariton optics using in-plane anisotropic van der Waals materials.","lang":"eng"}],"file_date_updated":"2021-10-27T14:16:06Z","issue":"41","external_id":{"arxiv":["2103.10852"],"isi":["000704912700024"]},"intvolume":"         7","file":[{"relation":"main_file","access_level":"open_access","checksum":"0a470ef6a47d2b8a96ede4c4d28cfacd","creator":"cziletti","content_type":"application/pdf","date_updated":"2021-10-27T14:16:06Z","file_id":"10189","success":1,"file_size":2441163,"date_created":"2021-10-27T14:16:06Z","file_name":"2021_ScienceAdv_Martin-Sanchez.pdf"}],"_id":"10177","status":"public","oa_version":"Published Version","isi":1,"day":"08","arxiv":1,"publisher":"American Association for the Advancement of Science","citation":{"chicago":"Martín-Sánchez, Javier, Jiahua Duan, Javier Taboada-Gutiérrez, Gonzalo Álvarez-Pérez, Kirill V. Voronin, Ivan Prieto Gonzalez, Weiliang Ma, et al. “Focusing of In-Plane Hyperbolic Polaritons in van Der Waals Crystals with Tailored Infrared Nanoantennas.” <i>Science Advances</i>. American Association for the Advancement of Science, 2021. <a href=\"https://doi.org/10.1126/sciadv.abj0127\">https://doi.org/10.1126/sciadv.abj0127</a>.","mla":"Martín-Sánchez, Javier, et al. “Focusing of In-Plane Hyperbolic Polaritons in van Der Waals Crystals with Tailored Infrared Nanoantennas.” <i>Science Advances</i>, vol. 7, no. 41, abj0127, American Association for the Advancement of Science, 2021, doi:<a href=\"https://doi.org/10.1126/sciadv.abj0127\">10.1126/sciadv.abj0127</a>.","short":"J. Martín-Sánchez, J. Duan, J. Taboada-Gutiérrez, G. Álvarez-Pérez, K.V. Voronin, I. Prieto Gonzalez, W. Ma, Q. Bao, V.S. Volkov, R. Hillenbrand, A.Y. Nikitin, P. Alonso-González, Science Advances 7 (2021).","ama":"Martín-Sánchez J, Duan J, Taboada-Gutiérrez J, et al. Focusing of in-plane hyperbolic polaritons in van der Waals crystals with tailored infrared nanoantennas. <i>Science Advances</i>. 2021;7(41). doi:<a href=\"https://doi.org/10.1126/sciadv.abj0127\">10.1126/sciadv.abj0127</a>","ista":"Martín-Sánchez J, Duan J, Taboada-Gutiérrez J, Álvarez-Pérez G, Voronin KV, Prieto Gonzalez I, Ma W, Bao Q, Volkov VS, Hillenbrand R, Nikitin AY, Alonso-González P. 2021. Focusing of in-plane hyperbolic polaritons in van der Waals crystals with tailored infrared nanoantennas. Science Advances. 7(41), abj0127.","ieee":"J. Martín-Sánchez <i>et al.</i>, “Focusing of in-plane hyperbolic polaritons in van der Waals crystals with tailored infrared nanoantennas,” <i>Science Advances</i>, vol. 7, no. 41. American Association for the Advancement of Science, 2021.","apa":"Martín-Sánchez, J., Duan, J., Taboada-Gutiérrez, J., Álvarez-Pérez, G., Voronin, K. V., Prieto Gonzalez, I., … Alonso-González, P. (2021). Focusing of in-plane hyperbolic polaritons in van der Waals crystals with tailored infrared nanoantennas. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.abj0127\">https://doi.org/10.1126/sciadv.abj0127</a>"},"acknowledgement":"J.M.-S. acknowledges financial support from the Ramón y Cajal Program of the Government of Spain and FSE (RYC2018-026196-I) and the Spanish Ministry of Science and Innovation (State Plan for Scientific and Technical Research and Innovation grant number PID2019-110308GA-I00). P.A.-G. acknowledges support from the European Research Council under starting grant no. 715496, 2DNANOPTICA, and the Spanish Ministry of Science and Innovation (State Plan for Scientific and Technical Research and Innovation grant number PID2019-111156GB-I00). J.T.-G. acknowledges support through the Severo Ochoa Program from the Government of the Principality of Asturias (PA-18-PF-BP17-126). G.A.-P. acknowledges support through the Severo Ochoa Program from the Government of the Principality of Asturias (PA-20-PF-BP19-053). K.V.V. and V.S.V. acknowledge the financial support from the Ministry of Science and Higher Education of the Russian Federation (agreement no. 075-15-2021-606). A.Y.N. acknowledges the Spanish Ministry of Science, Innovation, and Universities (national projects MAT2017-88358-C3-3-R and PID2020-115221GB-C42) and the Basque Department of Education (PIBA-2020-1-0014). R.H. acknowledges financial support from the Spanish Ministry of Science, Innovation, and Universities (national project number RTI2018-094830-B-100 and project number MDM-2016-0618 of the Marie de Maeztu Units of Excellence Program) and the Basque Government (grant number IT1164-19).","year":"2021","oa":1,"title":"Focusing of in-plane hyperbolic polaritons in van der Waals crystals with tailored infrared nanoantennas","publication_identifier":{"eissn":["2375-2548"]},"type":"journal_article","doi":"10.1126/sciadv.abj0127","article_number":"abj0127","scopus_import":"1","publication":"Science Advances","author":[{"first_name":"Javier","full_name":"Martín-Sánchez, Javier","last_name":"Martín-Sánchez"},{"last_name":"Duan","full_name":"Duan, Jiahua","first_name":"Jiahua"},{"full_name":"Taboada-Gutiérrez, Javier","last_name":"Taboada-Gutiérrez","first_name":"Javier"},{"first_name":"Gonzalo","last_name":"Álvarez-Pérez","full_name":"Álvarez-Pérez, Gonzalo"},{"full_name":"Voronin, Kirill V.","last_name":"Voronin","first_name":"Kirill V."},{"orcid":"0000-0002-7370-5357","first_name":"Ivan","full_name":"Prieto Gonzalez, Ivan","last_name":"Prieto Gonzalez","id":"2A307FE2-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Weiliang","full_name":"Ma, Weiliang","last_name":"Ma"},{"first_name":"Qiaoliang","last_name":"Bao","full_name":"Bao, Qiaoliang"},{"first_name":"Valentyn S.","full_name":"Volkov, Valentyn S.","last_name":"Volkov"},{"first_name":"Rainer","last_name":"Hillenbrand","full_name":"Hillenbrand, Rainer"},{"first_name":"Alexey Y.","last_name":"Nikitin","full_name":"Nikitin, Alexey Y."},{"first_name":"Pablo","last_name":"Alonso-González","full_name":"Alonso-González, Pablo"}],"article_processing_charge":"Yes","date_created":"2021-10-24T22:01:33Z","quality_controlled":"1","publication_status":"published","department":[{"_id":"NanoFab"}],"language":[{"iso":"eng"}],"volume":7,"month":"10","license":"https://creativecommons.org/licenses/by-nc/4.0/"},{"oa":1,"title":"Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration","publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"pmid":1,"type":"journal_article","doi":"10.15252/embj.2021108714","article_number":"e108714","scopus_import":"1","publication":"EMBO Journal","author":[{"first_name":"Sunanjay","last_name":"Bajaj","full_name":"Bajaj, Sunanjay"},{"first_name":"Joshua A.","full_name":"Bagley, Joshua A.","last_name":"Bagley"},{"first_name":"Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","full_name":"Sommer, Christoph M"},{"first_name":"Abel","last_name":"Vertesy","full_name":"Vertesy, Abel"},{"last_name":"Nagumo Wong","full_name":"Nagumo Wong, Sakurako","first_name":"Sakurako"},{"first_name":"Veronica","full_name":"Krenn, Veronica","last_name":"Krenn"},{"last_name":"Lévi-Strauss","full_name":"Lévi-Strauss, Julie","first_name":"Julie"},{"first_name":"Juergen A.","full_name":"Knoblich, Juergen A.","last_name":"Knoblich"}],"article_processing_charge":"Yes (in subscription journal)","date_created":"2021-10-24T22:01:34Z","quality_controlled":"1","publication_status":"published","volume":40,"department":[{"_id":"Bio"}],"language":[{"iso":"eng"}],"month":"10","has_accepted_license":"1","date_published":"2021-10-18T00:00:00Z","ddc":["610"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.15252/embj.2021108714","article_type":"original","date_updated":"2023-08-14T08:05:23Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","abstract":[{"text":"Inhibitory GABAergic interneurons migrate over long distances from their extracortical origin into the developing cortex. In humans, this process is uniquely slow and prolonged, and it is unclear whether guidance cues unique to humans govern the various phases of this complex developmental process. Here, we use fused cerebral organoids to identify key roles of neurotransmitter signaling pathways in guiding the migratory behavior of human cortical interneurons. We use scRNAseq to reveal expression of GABA, glutamate, glycine, and serotonin receptors along distinct maturation trajectories across interneuron migration. We develop an image analysis software package, TrackPal, to simultaneously assess 48 parameters for entire migration tracks of individual cells. By chemical screening, we show that different modes of interneuron migration depend on distinct neurotransmitter signaling pathways, linking transcriptional maturation of interneurons with their migratory behavior. Altogether, our study provides a comprehensive quantitative analysis of human interneuron migration and its functional modulation by neurotransmitter signaling.","lang":"eng"}],"file_date_updated":"2021-12-13T14:54:14Z","issue":"23","external_id":{"pmid":["34661293"],"isi":["000708012800001"]},"intvolume":"        40","file":[{"creator":"alisjak","content_type":"application/pdf","date_updated":"2021-12-13T14:54:14Z","relation":"main_file","access_level":"open_access","checksum":"78d2d02e775322297e774f72810a41a4","date_created":"2021-12-13T14:54:14Z","file_size":7819881,"file_name":"2021_EMBO_Bajaj.pdf","file_id":"10541","success":1}],"status":"public","_id":"10179","oa_version":"Published Version","isi":1,"day":"18","publisher":"Embo Press","acknowledgement":"We thank all Knoblich laboratory members for continued support and discussions. We thank the IMP/IMBA BioOptics facility, particularly Pawel Pasierbek, Alberto Moreno Cencerrado and Gerald Schmauss, the IMP/IMBA Molecular Biology Service, in particular Robert Heinen, the IMP Bioinformatics facility, in particular Thomas Burkard, the Vienna Biocenter Core Facilities (VBCF) Histopathology facility, in particular Tamara Engelmaier, and the VBCF Next Generation Sequencing Facility, notably Volodymyr Shubchynskyy and Carmen Czepe. We would also like to thank Simon Haendeler for advice on statistical analyses, Jose Guzman for discussions and assistance with slice culture setups, Oliver L. Eichmueller for discussions and assistance with microscopy, and E.H. Gustafson, S. Wolfinger, and D. Reumann for technical assistance regarding generation of cerebral organoids. This project received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie fellowship agreement Nr.707109 awarded to J.A.B. Work in J.A.K.'s laboratory is supported by the Austrian Federal Ministry of Education, Science and Research, the Austrian Academy of Sciences, the City of Vienna, a Research Program of the Austrian Science Fund FWF (SFBF78 Stem Cell, F 7803-B) and a European Research Council (ERC) Advanced Grant under the European 20 Union’s Horizon 2020 program (grant agreement no. 695642).","year":"2021","citation":{"ista":"Bajaj S, Bagley JA, Sommer CM, Vertesy A, Nagumo Wong S, Krenn V, Lévi-Strauss J, Knoblich JA. 2021. Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. EMBO Journal. 40(23), e108714.","ama":"Bajaj S, Bagley JA, Sommer CM, et al. Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. <i>EMBO Journal</i>. 2021;40(23). doi:<a href=\"https://doi.org/10.15252/embj.2021108714\">10.15252/embj.2021108714</a>","ieee":"S. Bajaj <i>et al.</i>, “Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration,” <i>EMBO Journal</i>, vol. 40, no. 23. Embo Press, 2021.","apa":"Bajaj, S., Bagley, J. A., Sommer, C. M., Vertesy, A., Nagumo Wong, S., Krenn, V., … Knoblich, J. A. (2021). Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2021108714\">https://doi.org/10.15252/embj.2021108714</a>","chicago":"Bajaj, Sunanjay, Joshua A. Bagley, Christoph M Sommer, Abel Vertesy, Sakurako Nagumo Wong, Veronica Krenn, Julie Lévi-Strauss, and Juergen A. Knoblich. “Neurotransmitter Signaling Regulates Distinct Phases of Multimodal Human Interneuron Migration.” <i>EMBO Journal</i>. Embo Press, 2021. <a href=\"https://doi.org/10.15252/embj.2021108714\">https://doi.org/10.15252/embj.2021108714</a>.","mla":"Bajaj, Sunanjay, et al. “Neurotransmitter Signaling Regulates Distinct Phases of Multimodal Human Interneuron Migration.” <i>EMBO Journal</i>, vol. 40, no. 23, e108714, Embo Press, 2021, doi:<a href=\"https://doi.org/10.15252/embj.2021108714\">10.15252/embj.2021108714</a>.","short":"S. Bajaj, J.A. Bagley, C.M. Sommer, A. Vertesy, S. Nagumo Wong, V. Krenn, J. Lévi-Strauss, J.A. Knoblich, EMBO Journal 40 (2021)."}},{"intvolume":"       599","external_id":{"pmid":["34707283"],"isi":["000713338100006"]},"issue":"7884","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"Bio"}],"acknowledgement":"We thank N. Gnyliukh and L. Hörmayer for technical assistance and N. Paris for sharing PM-Cyto seeds. We gratefully acknowledge the Life Science, Machine Shop and Bioimaging Facilities of IST Austria. This project has received funding from the European Research Council Advanced Grant (ETAP-742985) and the Austrian Science Fund (FWF) under I 3630-B25 to J.F., the National Institutes of Health (GM067203) to W.M.G., the Netherlands Organization for Scientific Research (NWO; VIDI-864.13.001), Research Foundation-Flanders (FWO; Odysseus II G0D0515N) and a European Research Council Starting Grant (TORPEDO-714055) to W.S. and B.D.R., the VICI grant (865.14.001) from the Netherlands Organization for Scientific Research to M.R. and D.W., the Australian Research Council and China National Distinguished Expert Project (WQ20174400441) to S.S., the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and 20H05910), the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement no. 665385 and the DOC Fellowship of the Austrian Academy of Sciences to L.L., and the China Scholarship Council to J.C.","year":"2021","citation":{"ieee":"L. Li <i>et al.</i>, “Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth,” <i>Nature</i>, vol. 599, no. 7884. Springer Nature, pp. 273–277, 2021.","apa":"Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez Solovey, L., Merrin, J., … Friml, J. (2021). Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-021-04037-6\">https://doi.org/10.1038/s41586-021-04037-6</a>","ama":"Li L, Verstraeten I, Roosjen M, et al. Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth. <i>Nature</i>. 2021;599(7884):273-277. doi:<a href=\"https://doi.org/10.1038/s41586-021-04037-6\">10.1038/s41586-021-04037-6</a>","ista":"Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez Solovey L, Merrin J, Chen J, Shabala L, Smet W, Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D, Kinoshita T, Gray WM, Friml J. 2021. Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth. Nature. 599(7884), 273–277.","short":"L. Li, I. Verstraeten, M. Roosjen, K. Takahashi, L. Rodriguez Solovey, J. Merrin, J. Chen, L. Shabala, W. Smet, H. Ren, S. Vanneste, S. Shabala, B. De Rybel, D. Weijers, T. Kinoshita, W.M. Gray, J. Friml, Nature 599 (2021) 273–277.","mla":"Li, Lanxin, et al. “Cell Surface and Intracellular Auxin Signalling for H<sup>+</sup> Fluxes in Root Growth.” <i>Nature</i>, vol. 599, no. 7884, Springer Nature, 2021, pp. 273–77, doi:<a href=\"https://doi.org/10.1038/s41586-021-04037-6\">10.1038/s41586-021-04037-6</a>.","chicago":"Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez Solovey, Jack Merrin, Jian Chen, et al. “Cell Surface and Intracellular Auxin Signalling for H<sup>+</sup> Fluxes in Root Growth.” <i>Nature</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41586-021-04037-6\">https://doi.org/10.1038/s41586-021-04037-6</a>."},"publisher":"Springer Nature","day":"11","isi":1,"oa_version":"Preprint","status":"public","_id":"10223","article_type":"original","fulldoi":"https://doi.org/10.1038/s41586-021-04037-6","keyword":["Multidisciplinary"],"date_published":"2021-11-11T00:00:00Z","ec_funded":1,"abstract":[{"text":"Growth regulation tailors development in plants to their environment. A prominent example of this is the response to gravity, in which shoots bend up and roots bend down1. This paradox is based on opposite effects of the phytohormone auxin, which promotes cell expansion in shoots while inhibiting it in roots via a yet unknown cellular mechanism2. Here, by combining microfluidics, live imaging, genetic engineering and phosphoproteomics in Arabidopsis thaliana, we advance understanding of how auxin inhibits root growth. We show that auxin activates two distinct, antagonistically acting signalling pathways that converge on rapid regulation of apoplastic pH, a causative determinant of growth. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts with and mediates phosphorylation and activation of plasma membrane H+-ATPases for apoplast acidification, while intracellular canonical auxin signalling promotes net cellular H+ influx, causing apoplast alkalinization. Simultaneous activation of these two counteracting mechanisms poises roots for rapid, fine-tuned growth modulation in navigating complex soil environments.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2025-07-10T11:49:46Z","related_material":{"record":[{"relation":"earlier_version","id":"10095","status":"public"}],"link":[{"url":"https://ist.ac.at/en/news/stop-and-grow/","relation":"press_release","description":"News on IST Webpage"}]},"quality_controlled":"1","project":[{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF"},{"call_identifier":"H2020","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385"},{"grant_number":"25351","name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root","_id":"26B4D67E-B435-11E9-9278-68D0E5697425"}],"date_created":"2021-11-07T23:01:25Z","article_processing_charge":"No","month":"11","volume":599,"department":[{"_id":"JiFr"},{"_id":"NanoFab"}],"language":[{"iso":"eng"}],"publication_status":"published","page":"273-277","main_file_link":[{"url":"https://www.doi.org/10.21203/rs.3.rs-266395/v3","open_access":"1"}],"doi":"10.1038/s41586-021-04037-6","type":"journal_article","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"pmid":1,"title":"Cell surface and intracellular auxin signalling for H<sup>+</sup> fluxes in root growth","oa":1,"author":[{"orcid":"0000-0002-5607-272X","first_name":"Lanxin","full_name":"Li, Lanxin","last_name":"Li","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Verstraeten","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","full_name":"Verstraeten, Inge","first_name":"Inge","orcid":"0000-0001-7241-2328"},{"full_name":"Roosjen, Mark","last_name":"Roosjen","first_name":"Mark"},{"first_name":"Koji","full_name":"Takahashi, Koji","last_name":"Takahashi"},{"orcid":"0000-0002-7244-7237","first_name":"Lesia","last_name":"Rodriguez Solovey","id":"3922B506-F248-11E8-B48F-1D18A9856A87","full_name":"Rodriguez Solovey, Lesia"},{"last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","first_name":"Jack"},{"full_name":"Chen, Jian","last_name":"Chen","first_name":"Jian"},{"first_name":"Lana","last_name":"Shabala","full_name":"Shabala, Lana"},{"full_name":"Smet, Wouter","last_name":"Smet","first_name":"Wouter"},{"first_name":"Hong","full_name":"Ren, Hong","last_name":"Ren"},{"first_name":"Steffen","last_name":"Vanneste","full_name":"Vanneste, Steffen"},{"first_name":"Sergey","full_name":"Shabala, Sergey","last_name":"Shabala"},{"first_name":"Bert","full_name":"De Rybel, Bert","last_name":"De Rybel"},{"first_name":"Dolf","last_name":"Weijers","full_name":"Weijers, Dolf"},{"full_name":"Kinoshita, Toshinori","last_name":"Kinoshita","first_name":"Toshinori"},{"first_name":"William M.","full_name":"Gray, William M.","last_name":"Gray"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"publication":"Nature","scopus_import":"1","corr_author":"1"},{"acknowledgement":"This EQIPD project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement no. 777364. This Joint Undertaking receives support from the European Union’s Horizon 2020 research and innovation program and EFPIA. LR was supported by the Faculty of Biology and Medicine, University of Lausanne. VV was supported by Biocenter Finland and the Jane and Aatos Erkko Foundation. CP and IKB received funding from the Federal Ministry of Education and Research (BMBF, grant 01PW18001). SB from the Vienna BioCenter Core Facilities (VBCF) Preclinical Phenotyping Facility acknowledges funding from the Austrian Federal Ministry of Education, Science & Research; and the City of Vienna. MT is an incumbent of the Carolito Stiftung Research Fellow Chair in Neurodegenerative Diseases. We thank Dr. Katja Kivinen (Helsinki Institute of Life Science) for discussions and feedback.","citation":{"ista":"Restivo L, Gerlach B, Tsoory M, Bikovski L, Badurek S, Pitzer C, Kos-Braun IC, Mausset-Bonnefont ALM, Ward J, Schunn M, Noldus LPJJ, Bespalov A, Voikar V. 2021. Towards best practices in research: Role of academic core facilities. EMBO Reports. 22, e53824.","ama":"Restivo L, Gerlach B, Tsoory M, et al. Towards best practices in research: Role of academic core facilities. <i>EMBO Reports</i>. 2021;22. doi:<a href=\"https://doi.org/10.15252/embr.202153824\">10.15252/embr.202153824</a>","apa":"Restivo, L., Gerlach, B., Tsoory, M., Bikovski, L., Badurek, S., Pitzer, C., … Voikar, V. (2021). Towards best practices in research: Role of academic core facilities. <i>EMBO Reports</i>. EMBO Press. <a href=\"https://doi.org/10.15252/embr.202153824\">https://doi.org/10.15252/embr.202153824</a>","ieee":"L. Restivo <i>et al.</i>, “Towards best practices in research: Role of academic core facilities,” <i>EMBO Reports</i>, vol. 22. EMBO Press, 2021.","chicago":"Restivo, Leonardo, Björn Gerlach, Michael Tsoory, Lior Bikovski, Sylvia Badurek, Claudia Pitzer, Isabelle C. Kos-Braun, et al. “Towards Best Practices in Research: Role of Academic Core Facilities.” <i>EMBO Reports</i>. EMBO Press, 2021. <a href=\"https://doi.org/10.15252/embr.202153824\">https://doi.org/10.15252/embr.202153824</a>.","short":"L. Restivo, B. Gerlach, M. Tsoory, L. Bikovski, S. Badurek, C. Pitzer, I.C. Kos-Braun, A.L.M. Mausset-Bonnefont, J. Ward, M. Schunn, L.P.J.J. Noldus, A. Bespalov, V. Voikar, EMBO Reports 22 (2021).","mla":"Restivo, Leonardo, et al. “Towards Best Practices in Research: Role of Academic Core Facilities.” <i>EMBO Reports</i>, vol. 22, e53824, EMBO Press, 2021, doi:<a href=\"https://doi.org/10.15252/embr.202153824\">10.15252/embr.202153824</a>."},"year":"2021","publisher":"EMBO Press","day":"04","isi":1,"oa_version":"Published Version","status":"public","_id":"10283","file":[{"file_name":"2021_EmboReports_Restivo.pdf","file_size":488583,"date_created":"2022-05-16T07:07:41Z","success":1,"file_id":"11381","content_type":"application/pdf","date_updated":"2022-05-16T07:07:41Z","creator":"dernst","checksum":"74743baa6ef431ef60c3de3bc4da045a","access_level":"open_access","relation":"main_file"}],"intvolume":"        22","external_id":{"isi":["000714350000001"]},"abstract":[{"text":"During the past decade, the scientific community and outside observers have noted a concerning lack of rigor and transparency in preclinical research that led to talk of a “reproducibility crisis” in the life sciences (Baker, 2016; Bespalov & Steckler, 2018; Heddleston et al, 2021). Various measures have been proposed to address the problem: from better training of scientists to more oversight to expanded publishing practices such as preregistration of studies. The recently published EQIPD (Enhancing Quality in Preclinical Data) System is, to date, the largest initiative that aims to establish a systematic approach for increasing the robustness and reliability of biomedical research (Bespalov et al, 2021). However, promoting a cultural change in research practices warrants a broad adoption of the Quality System and its underlying philosophy. It is here that academic Core Facilities (CF), research service providers at universities and research institutions, can make a difference. It is fair to assume that a significant fraction of published data originated from experiments that were designed, run, or analyzed in CFs. These academic services play an important role in the research ecosystem by offering access to cutting-edge equipment and by developing and testing novel techniques and methods that impact research in the academic and private sectors alike (Bikovski et al, 2020). Equipment and infrastructure are not the only value: CFs employ competent personnel with profound knowledge and practical experience of the specific field of interest: animal behavior, imaging, crystallography, genomics, and so on. Thus, CFs are optimally positioned to address concerns about the quality and robustness of preclinical research.","lang":"eng"}],"file_date_updated":"2022-05-16T07:07:41Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2023-08-14T11:47:35Z","fulldoi":"https://doi.org/10.15252/embr.202153824","article_type":"original","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"ddc":["570"],"has_accepted_license":"1","date_published":"2021-11-04T00:00:00Z","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","month":"11","language":[{"iso":"eng"}],"department":[{"_id":"PreCl"}],"volume":22,"publication_status":"published","quality_controlled":"1","date_created":"2021-11-14T23:01:24Z","article_processing_charge":"Yes (in subscription journal)","author":[{"full_name":"Restivo, Leonardo","last_name":"Restivo","first_name":"Leonardo"},{"full_name":"Gerlach, Björn","last_name":"Gerlach","first_name":"Björn"},{"full_name":"Tsoory, Michael","last_name":"Tsoory","first_name":"Michael"},{"last_name":"Bikovski","full_name":"Bikovski, Lior","first_name":"Lior"},{"last_name":"Badurek","full_name":"Badurek, Sylvia","first_name":"Sylvia"},{"full_name":"Pitzer, Claudia","last_name":"Pitzer","first_name":"Claudia"},{"full_name":"Kos-Braun, Isabelle C.","last_name":"Kos-Braun","first_name":"Isabelle C."},{"last_name":"Mausset-Bonnefont","full_name":"Mausset-Bonnefont, Anne Laure Mj","first_name":"Anne Laure Mj"},{"last_name":"Ward","full_name":"Ward, Jonathan","first_name":"Jonathan"},{"orcid":"0000-0003-4326-5300","first_name":"Michael","last_name":"Schunn","id":"4272DB4A-F248-11E8-B48F-1D18A9856A87","full_name":"Schunn, Michael"},{"last_name":"Noldus","full_name":"Noldus, Lucas P.J.J.","first_name":"Lucas P.J.J."},{"last_name":"Bespalov","full_name":"Bespalov, Anton","first_name":"Anton"},{"full_name":"Voikar, Vootele","last_name":"Voikar","first_name":"Vootele"}],"publication":"EMBO Reports","scopus_import":"1","article_number":"e53824","doi":"10.15252/embr.202153824","type":"journal_article","publication_identifier":{"eissn":["1469-3178"],"issn":["1469-221X"]},"oa":1,"title":"Towards best practices in research: Role of academic core facilities"},{"oa_version":"Published Version","_id":"10607","status":"public","publisher":"Elsevier","acknowledgement":"This study was supported by grants of the Austrian Science Fund (FWF) F4414 and W1206-08. Electron microscopy was performed at the Scientific Service Units (SSU) of IST-Austria through resources provided by the Electron Microscopy Facility.","citation":{"mla":"Venezia, Serena, et al. “Toll-like Receptor 4 Deficiency Facilitates α-Synuclein Propagation and Neurodegeneration in a Mouse Model of Prodromal Parkinson’s Disease.” <i>Parkinsonism &#38; Related Disorders</i>, vol. 91, Elsevier, 2021, pp. 59–65, doi:<a href=\"https://doi.org/10.1016/j.parkreldis.2021.09.007\">10.1016/j.parkreldis.2021.09.007</a>.","short":"S. Venezia, W. Kaufmann, G.K. Wenning, N. Stefanova, Parkinsonism &#38; Related Disorders 91 (2021) 59–65.","chicago":"Venezia, Serena, Walter Kaufmann, Gregor K. Wenning, and Nadia Stefanova. “Toll-like Receptor 4 Deficiency Facilitates α-Synuclein Propagation and Neurodegeneration in a Mouse Model of Prodromal Parkinson’s Disease.” <i>Parkinsonism &#38; Related Disorders</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.parkreldis.2021.09.007\">https://doi.org/10.1016/j.parkreldis.2021.09.007</a>.","apa":"Venezia, S., Kaufmann, W., Wenning, G. K., &#38; Stefanova, N. (2021). Toll-like receptor 4 deficiency facilitates α-synuclein propagation and neurodegeneration in a mouse model of prodromal Parkinson’s disease. <i>Parkinsonism &#38; Related Disorders</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.parkreldis.2021.09.007\">https://doi.org/10.1016/j.parkreldis.2021.09.007</a>","ieee":"S. Venezia, W. Kaufmann, G. K. Wenning, and N. Stefanova, “Toll-like receptor 4 deficiency facilitates α-synuclein propagation and neurodegeneration in a mouse model of prodromal Parkinson’s disease,” <i>Parkinsonism &#38; Related Disorders</i>, vol. 91. Elsevier, pp. 59–65, 2021.","ama":"Venezia S, Kaufmann W, Wenning GK, Stefanova N. Toll-like receptor 4 deficiency facilitates α-synuclein propagation and neurodegeneration in a mouse model of prodromal Parkinson’s disease. <i>Parkinsonism &#38; Related Disorders</i>. 2021;91:59-65. doi:<a href=\"https://doi.org/10.1016/j.parkreldis.2021.09.007\">10.1016/j.parkreldis.2021.09.007</a>","ista":"Venezia S, Kaufmann W, Wenning GK, Stefanova N. 2021. Toll-like receptor 4 deficiency facilitates α-synuclein propagation and neurodegeneration in a mouse model of prodromal Parkinson’s disease. Parkinsonism &#38; Related Disorders. 91, 59–65."},"year":"2021","isi":1,"day":"01","intvolume":"        91","file":[{"creator":"alisjak","content_type":"application/pdf","date_updated":"2022-01-10T13:41:40Z","relation":"main_file","access_level":"open_access","checksum":"360681585acb51e80d17c6b213c56b55","file_size":6848513,"date_created":"2022-01-10T13:41:40Z","file_name":"2021_Parkinsonism_Venezia.pdf","file_id":"10612","success":1}],"external_id":{"isi":["000701142900012"],"pmid":["34530328"]},"date_updated":"2023-08-17T06:36:01Z","abstract":[{"lang":"eng","text":"The evidence linking innate immunity mechanisms and neurodegenerative diseases is growing, but the specific mechanisms are incompletely understood. Experimental data suggest that microglial TLR4 mediates the uptake and clearance of α-synuclein also termed synucleinophagy. The accumulation of misfolded α-synuclein throughout the brain is central to Parkinson's disease (PD). The distribution and progression of the pathology is often attributed to the propagation of α-synuclein. Here, we apply a classical α-synuclein propagation model of prodromal PD in wild type and TLR4 deficient mice to study the role of TLR4 in the progression of the disease. Our data suggest that TLR4 deficiency facilitates the α-synuclein seed spreading associated with reduced lysosomal activity of microglia. Three months after seed inoculation, more pronounced proteinase K-resistant α-synuclein inclusion pathology is observed in mice with TLR4 deficiency. The facilitated propagation of α-synuclein is associated with early loss of dopamine transporter (DAT) signal in the striatum and loss of dopaminergic neurons in substantia nigra pars compacta of TLR4 deficient mice. These new results support TLR4 signaling as a putative target for disease modification to slow the progression of PD and related disorders."}],"file_date_updated":"2022-01-10T13:41:40Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_type":"original","fulldoi":"https://doi.org/10.1016/j.parkreldis.2021.09.007","has_accepted_license":"1","date_published":"2021-10-01T00:00:00Z","ddc":["610"],"publication_status":"published","language":[{"iso":"eng"}],"volume":91,"department":[{"_id":"EM-Fac"}],"page":"59-65","month":"10","article_processing_charge":"No","date_created":"2022-01-09T23:01:26Z","quality_controlled":"1","scopus_import":"1","author":[{"first_name":"Serena","last_name":"Venezia","full_name":"Venezia, Serena"},{"first_name":"Walter","orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann"},{"last_name":"Wenning","full_name":"Wenning, Gregor K.","first_name":"Gregor K."},{"first_name":"Nadia","full_name":"Stefanova, Nadia","last_name":"Stefanova"}],"publication":"Parkinsonism & Related Disorders","type":"journal_article","doi":"10.1016/j.parkreldis.2021.09.007","oa":1,"title":"Toll-like receptor 4 deficiency facilitates α-synuclein propagation and neurodegeneration in a mouse model of prodromal Parkinson's disease","publication_identifier":{"issn":["1353-8020"],"eissn":["1873-5126"]},"pmid":1},{"project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"},{"_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734"}],"quality_controlled":"1","article_processing_charge":"Yes (via OA deal)","date_created":"2020-09-28T08:59:28Z","month":"01","page":"351-369","publication_status":"published","volume":229,"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"EvBe"}],"oa":1,"title":"Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana","publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"pmid":1,"type":"journal_article","doi":"10.1111/nph.16887","publication":"New Phytologist","author":[{"first_name":"Hongjiang","orcid":"0000-0001-5039-9660","full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","last_name":"Li"},{"first_name":"Daniel","orcid":"0000-0002-6862-1247","id":"49E91952-F248-11E8-B48F-1D18A9856A87","last_name":"von Wangenheim","full_name":"von Wangenheim, Daniel"},{"first_name":"Xixi","orcid":"0000-0001-7048-4627","last_name":"Zhang","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","full_name":"Zhang, Xixi"},{"orcid":"0000-0002-0471-8285","first_name":"Shutang","last_name":"Tan","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang"},{"full_name":"Darwish-Miranda, Nasser","id":"39CD9926-F248-11E8-B48F-1D18A9856A87","last_name":"Darwish-Miranda","first_name":"Nasser","orcid":"0000-0002-8821-8236"},{"last_name":"Naramoto","full_name":"Naramoto, Satoshi","first_name":"Satoshi"},{"full_name":"Wabnik, Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","last_name":"Wabnik","orcid":"0000-0001-7263-0560","first_name":"Krzysztof T"},{"full_name":"de Rycke, Riet","last_name":"de Rycke","first_name":"Riet"},{"full_name":"Kaufmann, Walter","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","orcid":"0000-0001-9735-5315"},{"last_name":"Gütl","id":"381929CE-F248-11E8-B48F-1D18A9856A87","full_name":"Gütl, Daniel J","first_name":"Daniel J"},{"first_name":"Ricardo","full_name":"Tejos, Ricardo","last_name":"Tejos"},{"full_name":"Grones, Peter","last_name":"Grones","id":"399876EC-F248-11E8-B48F-1D18A9856A87","first_name":"Peter"},{"first_name":"Meiyu","last_name":"Ke","full_name":"Ke, Meiyu"},{"first_name":"Xu","full_name":"Chen, Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","last_name":"Chen"},{"full_name":"Dettmer, Jan","last_name":"Dettmer","first_name":"Jan"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"scopus_import":"1","issue":"1","external_id":{"isi":["000570187900001"],"pmid":["32810889"]},"intvolume":"       229","file":[{"checksum":"b45621607b4cab97eeb1605ab58e896e","access_level":"open_access","relation":"main_file","creator":"dernst","content_type":"application/pdf","date_updated":"2021-02-04T09:44:17Z","success":1,"file_id":"9084","file_size":4061962,"date_created":"2021-02-04T09:44:17Z","file_name":"2021_NewPhytologist_Li.pdf"}],"acknowledged_ssus":[{"_id":"Bio"}],"isi":1,"day":"01","publisher":"Wiley","acknowledgement":"We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg) for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1 mutant and the complementation lines, and Dr Patricia C. Zambryski (University of California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria) for assistance with imaging, group members for discussions, Martine De Cock for help in preparing the manuscript and Nataliia Gnyliukh for critical reading and revision of the manuscript. This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 742985) and Comisión Nacional de Investigación Científica y Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007‐2013) under REA grant agreement no. 291734.","citation":{"apa":"Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto, S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>","ieee":"H. Li <i>et al.</i>, “Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 229, no. 1. Wiley, pp. 351–369, 2021.","ama":"Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;229(1):351-369. doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>","ista":"Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. New Phytologist. 229(1), 351–369.","mla":"Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 229, no. 1, Wiley, 2021, pp. 351–69, doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>.","short":"H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto, K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke, X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.","chicago":"Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>."},"year":"2021","status":"public","_id":"8582","oa_version":"Published Version","has_accepted_license":"1","date_published":"2021-01-01T00:00:00Z","ddc":["580"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1111/nph.16887","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear.\r\nHere, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze‐fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall components as well as connections between the cell wall and the plasma membrane.\r\nThis study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems."}],"ec_funded":1,"file_date_updated":"2021-02-04T09:44:17Z","date_updated":"2025-06-12T06:32:24Z"},{"issue":"6550","external_id":{"arxiv":["2008.02348"],"isi":["000677843100034"],"pmid":["34210881"]},"intvolume":"       373","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"}],"isi":1,"day":"02","arxiv":1,"publisher":"American Association for the Advancement of Science","citation":{"ama":"Valentini M, Peñaranda F, Hofmann AC, et al. Nontopological zero-bias peaks in full-shell nanowires induced by flux-tunable Andreev states. <i>Science</i>. 2021;373(6550). doi:<a href=\"https://doi.org/10.1126/science.abf1513\">10.1126/science.abf1513</a>","ista":"Valentini M, Peñaranda F, Hofmann AC, Brauns M, Hauschild R, Krogstrup P, San-Jose P, Prada E, Aguado R, Katsaros G. 2021. Nontopological zero-bias peaks in full-shell nanowires induced by flux-tunable Andreev states. Science. 373(6550), 82–88.","ieee":"M. Valentini <i>et al.</i>, “Nontopological zero-bias peaks in full-shell nanowires induced by flux-tunable Andreev states,” <i>Science</i>, vol. 373, no. 6550. American Association for the Advancement of Science, 2021.","apa":"Valentini, M., Peñaranda, F., Hofmann, A. C., Brauns, M., Hauschild, R., Krogstrup, P., … Katsaros, G. (2021). Nontopological zero-bias peaks in full-shell nanowires induced by flux-tunable Andreev states. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.abf1513\">https://doi.org/10.1126/science.abf1513</a>","chicago":"Valentini, Marco, Fernando Peñaranda, Andrea C Hofmann, Matthias Brauns, Robert Hauschild, Peter Krogstrup, Pablo San-Jose, Elsa Prada, Ramón Aguado, and Georgios Katsaros. “Nontopological Zero-Bias Peaks in Full-Shell Nanowires Induced by Flux-Tunable Andreev States.” <i>Science</i>. American Association for the Advancement of Science, 2021. <a href=\"https://doi.org/10.1126/science.abf1513\">https://doi.org/10.1126/science.abf1513</a>.","mla":"Valentini, Marco, et al. “Nontopological Zero-Bias Peaks in Full-Shell Nanowires Induced by Flux-Tunable Andreev States.” <i>Science</i>, vol. 373, no. 6550, 82–88, American Association for the Advancement of Science, 2021, doi:<a href=\"https://doi.org/10.1126/science.abf1513\">10.1126/science.abf1513</a>.","short":"M. Valentini, F. Peñaranda, A.C. Hofmann, M. Brauns, R. Hauschild, P. Krogstrup, P. San-Jose, E. Prada, R. Aguado, G. Katsaros, Science 373 (2021)."},"year":"2021","acknowledgement":"The authors thank A. Higginbotham, E. J. H. Lee and F. R. Martins for helpful discussions. This research was supported by the Scientific Service Units of IST Austria through resources provided by the MIBA Machine Shop and the nanofabrication facility; the NOMIS Foundation and Microsoft; the European Union’s Horizon 2020 research and innovation program under the Marie SklodowskaCurie grant agreement No 844511; the FETOPEN Grant Agreement No. 828948; the European Research Commission through the grant agreement HEMs-DAM No 716655; the Spanish Ministry of Science and Innovation through Grants PGC2018-097018-B-I00, PCI2018-093026, FIS2016-80434-P (AEI/FEDER, EU), RYC2011-09345 (Ram´on y Cajal Programme), and the Mar´ıa de Maeztu Programme for Units of Excellence in R&D (CEX2018-000805-M); the CSIC Research Platform on Quantum Technologies PTI-001.","status":"public","_id":"8910","oa_version":"Submitted Version","date_published":"2021-07-02T00:00:00Z","article_type":"original","fulldoi":"https://doi.org/10.1126/science.abf1513","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ec_funded":1,"abstract":[{"lang":"eng","text":"A semiconducting nanowire fully wrapped by a superconducting shell has been proposed as a platform for obtaining Majorana modes at small magnetic fields. In this study, we demonstrate that the appearance of subgap states in such structures is actually governed by the junction region in tunneling spectroscopy measurements and not the full-shell nanowire itself. Short tunneling regions never show subgap states, whereas longer junctions always do. This can be understood in terms of quantum dots forming in the junction and hosting Andreev levels in the Yu-Shiba-Rusinov regime. The intricate magnetic field dependence of the Andreev levels, through both the Zeeman and Little-Parks effects, may result in robust zero-bias peaks—features that could be easily misinterpreted as originating from Majorana zero modes but are unrelated to topological superconductivity."}],"date_updated":"2026-04-07T13:27:22Z","project":[{"_id":"262116AA-B435-11E9-9278-68D0E5697425","name":"Hybrid Semiconductor - Superconductor Quantum Devices"},{"_id":"26A151DA-B435-11E9-9278-68D0E5697425","name":"Majorana bound states in Ge/SiGe heterostructures","call_identifier":"H2020","grant_number":"844511"}],"related_material":{"record":[{"id":"9389","relation":"research_data","status":"public"},{"status":"public","id":"13286","relation":"dissertation_contains"}],"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/unfinding-a-split-electron/"}]},"quality_controlled":"1","article_processing_charge":"No","date_created":"2020-12-02T10:51:52Z","month":"07","publication_status":"published","language":[{"iso":"eng"}],"volume":373,"department":[{"_id":"GeKa"},{"_id":"Bio"}],"article_number":"82-88","title":"Nontopological zero-bias peaks in full-shell nanowires induced by flux-tunable Andreev states","oa":1,"pmid":1,"publication_identifier":{"eissn":["1095-9203"],"issn":["0036-8075"]},"type":"journal_article","doi":"10.1126/science.abf1513","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/2008.02348"}],"publication":"Science","author":[{"first_name":"Marco","last_name":"Valentini","id":"C0BB2FAC-D767-11E9-B658-BC13E6697425","full_name":"Valentini, Marco"},{"last_name":"Peñaranda","full_name":"Peñaranda, Fernando","first_name":"Fernando"},{"first_name":"Andrea C","last_name":"Hofmann","id":"340F461A-F248-11E8-B48F-1D18A9856A87","full_name":"Hofmann, Andrea C"},{"last_name":"Brauns","id":"33F94E3C-F248-11E8-B48F-1D18A9856A87","full_name":"Brauns, Matthias","first_name":"Matthias"},{"full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","orcid":"0000-0001-9843-3522","first_name":"Robert"},{"full_name":"Krogstrup, Peter","last_name":"Krogstrup","first_name":"Peter"},{"last_name":"San-Jose","full_name":"San-Jose, Pablo","first_name":"Pablo"},{"first_name":"Elsa","full_name":"Prada, Elsa","last_name":"Prada"},{"first_name":"Ramón","last_name":"Aguado","full_name":"Aguado, Ramón"},{"full_name":"Katsaros, Georgios","id":"38DB5788-F248-11E8-B48F-1D18A9856A87","last_name":"Katsaros","first_name":"Georgios","orcid":"0000-0001-8342-202X"}],"scopus_import":"1"},{"title":"Pathophysiological mechanisms of liver injury in COVID-19","oa":1,"pmid":1,"publication_identifier":{"eissn":["1478-3231"],"issn":["1478-3223"]},"type":"journal_article","doi":"10.1111/liv.14730","scopus_import":"1","publication":"Liver International","author":[{"full_name":"Nardo, Alexander D.","last_name":"Nardo","first_name":"Alexander D."},{"first_name":"Mathias","full_name":"Schneeweiss-Gleixner, Mathias","last_name":"Schneeweiss-Gleixner"},{"last_name":"Bakail","id":"FB3C3F8E-522F-11EA-B186-22963DDC885E","full_name":"Bakail, May M","orcid":"0000-0002-9592-1587","first_name":"May M"},{"full_name":"Dixon, Emmanuel D.","last_name":"Dixon","first_name":"Emmanuel D."},{"first_name":"Sigurd F.","full_name":"Lax, Sigurd F.","last_name":"Lax"},{"last_name":"Trauner","full_name":"Trauner, Michael","first_name":"Michael"}],"article_processing_charge":"No","date_created":"2020-12-06T23:01:16Z","quality_controlled":"1","page":"20-32","publication_status":"published","department":[{"_id":"CampIT"}],"language":[{"iso":"eng"}],"volume":41,"month":"01","has_accepted_license":"1","date_published":"2021-01-01T00:00:00Z","ddc":["570"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1111/liv.14730","article_type":"original","date_updated":"2025-06-12T06:33:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2021-02-04T12:01:45Z","abstract":[{"text":"The recent outbreak of coronavirus disease 2019 (COVID‐19), caused by the Severe Acute Respiratory Syndrome Coronavirus‐2 (SARS‐CoV‐2) has resulted in a world‐wide pandemic. Disseminated lung injury with the development of acute respiratory distress syndrome (ARDS) is the main cause of mortality in COVID‐19. Although liver failure does not seem to occur in the absence of pre‐existing liver disease, hepatic involvement in COVID‐19 may correlate with overall disease severity and serve as a prognostic factor for the development of ARDS. The spectrum of liver injury in COVID‐19 may range from direct infection by SARS‐CoV‐2, indirect involvement by systemic inflammation, hypoxic changes, iatrogenic causes such as drugs and ventilation to exacerbation of underlying liver disease. This concise review discusses the potential pathophysiological mechanisms for SARS‐CoV‐2 hepatic tropism as well as acute and possibly long‐term liver injury in COVID‐19.","lang":"eng"}],"issue":"1","external_id":{"isi":["000594239200001"],"pmid":["33190346"]},"intvolume":"        41","file":[{"file_id":"9091","success":1,"date_created":"2021-02-04T12:01:45Z","file_size":930414,"file_name":"2021_Liver_Nardo.pdf","relation":"main_file","checksum":"6e4f21b77ef22c854e016240974fc473","access_level":"open_access","creator":"dernst","date_updated":"2021-02-04T12:01:45Z","content_type":"application/pdf"}],"_id":"8927","status":"public","oa_version":"Published Version","isi":1,"day":"01","publisher":"Wiley","year":"2021","citation":{"short":"A.D. Nardo, M. Schneeweiss-Gleixner, M.M. Bakail, E.D. Dixon, S.F. Lax, M. Trauner, Liver International 41 (2021) 20–32.","mla":"Nardo, Alexander D., et al. “Pathophysiological Mechanisms of Liver Injury in COVID-19.” <i>Liver International</i>, vol. 41, no. 1, Wiley, 2021, pp. 20–32, doi:<a href=\"https://doi.org/10.1111/liv.14730\">10.1111/liv.14730</a>.","chicago":"Nardo, Alexander D., Mathias Schneeweiss-Gleixner, May M Bakail, Emmanuel D. Dixon, Sigurd F. Lax, and Michael Trauner. “Pathophysiological Mechanisms of Liver Injury in COVID-19.” <i>Liver International</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/liv.14730\">https://doi.org/10.1111/liv.14730</a>.","ieee":"A. D. Nardo, M. Schneeweiss-Gleixner, M. M. Bakail, E. D. Dixon, S. F. Lax, and M. Trauner, “Pathophysiological mechanisms of liver injury in COVID-19,” <i>Liver International</i>, vol. 41, no. 1. Wiley, pp. 20–32, 2021.","apa":"Nardo, A. D., Schneeweiss-Gleixner, M., Bakail, M. M., Dixon, E. D., Lax, S. F., &#38; Trauner, M. (2021). Pathophysiological mechanisms of liver injury in COVID-19. <i>Liver International</i>. Wiley. <a href=\"https://doi.org/10.1111/liv.14730\">https://doi.org/10.1111/liv.14730</a>","ama":"Nardo AD, Schneeweiss-Gleixner M, Bakail MM, Dixon ED, Lax SF, Trauner M. Pathophysiological mechanisms of liver injury in COVID-19. <i>Liver International</i>. 2021;41(1):20-32. doi:<a href=\"https://doi.org/10.1111/liv.14730\">10.1111/liv.14730</a>","ista":"Nardo AD, Schneeweiss-Gleixner M, Bakail MM, Dixon ED, Lax SF, Trauner M. 2021. Pathophysiological mechanisms of liver injury in COVID-19. Liver International. 41(1), 20–32."},"acknowledgement":"This work was supported by grant F7310‐B21 from the Austrian Science Foundation (to MT). We thank Jelena Remetic, Claudia D. Fuchs, Veronika Mlitz and Daniel Steinacher, for their valuable input and discussion. Figure 1 and Figure 2 have been created with BioRender.com."},{"fulldoi":"https://doi.org/10.3390/nano11010120","article_type":"original","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["620"],"date_published":"2021-01-07T00:00:00Z","has_accepted_license":"1","file_date_updated":"2021-01-25T08:02:32Z","abstract":[{"lang":"eng","text":"Layered materials in which individual atomic layers are bonded by weak van der Waals forces (vdW materials) constitute one of the most prominent platforms for materials research. Particularly, polar vdW crystals, such as hexagonal boron nitride (h-BN), alpha-molybdenum trioxide (α-MoO3) or alpha-vanadium pentoxide (α-V2O5), have received significant attention in nano-optics, since they support phonon polaritons (PhPs)―light coupled to lattice vibrations― with strong electromagnetic confinement and low optical losses. Recently, correlative far- and near-field studies of α-MoO3 have been demonstrated as an effective strategy to accurately extract the permittivity of this material. Here, we use this accurately characterized and low-loss polaritonic material to sense its local dielectric environment, namely silica (SiO2), one of the most widespread substrates in nanotechnology. By studying the propagation of PhPs on α-MoO3 flakes with different thicknesses laying on SiO2 substrates via near-field microscopy (s-SNOM), we extract locally the infrared permittivity of SiO2. Our work reveals PhPs nanoimaging as a versatile method for the quantitative characterization of the local optical properties of dielectric substrates, crucial for understanding and predicting the response of nanomaterials and for the future scalability of integrated nanophotonic devices. "}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-04-02T13:57:24Z","file":[{"creator":"dernst","date_updated":"2021-01-25T08:02:32Z","content_type":"application/pdf","access_level":"open_access","checksum":"1edc13eeda83df5cd9fff9504727b1f5","relation":"main_file","file_size":2730267,"date_created":"2021-01-25T08:02:32Z","file_name":"2020_Nanomaterials_Aguilar_Merino.pdf","success":1,"file_id":"9042"}],"intvolume":"        11","external_id":{"isi":["000610636600001"],"pmid":["33430225"]},"issue":"1","acknowledgement":"P.A.-M. acknowledges financial support through JAE Intro program from the Superior\r\nCouncil of Scientific Investigations and the Spanish Ministry of Science and Innovation (grant number JAEINT_20_00589). G.Á.-P. and J.T.-G. acknowledge financial support through the Severo Ochoa Program from the Government of the Principality of Asturias (grant numbers PA-20-PF-BP19-053 and PA-18-PF-BP17-126, respectively). J.M.-S. acknowledges financial support from the Ramón y Cajal Program of the Government of Spain (RYC2018-026196-I) and the Spanish Ministry of Science and Innovation (State Plan for Scientific and Technical Research and Innovation grant number PID2019-110308GA-I00). P.A.-G. acknowledges support from the European Research Council under starting grant no. 715496, 2DNANOPTICA and the Spanish Ministry of Science and Innovation (State Plan for Scientific and Technical Research and Innovation grant number PID2019-111156GB-I00).","citation":{"ama":"Aguilar-Merino P, Álvarez-Pérez G, Taboada-Gutiérrez J, et al. Extracting the infrared permittivity of SiO2 substrates locally by near-field imaging of phonon polaritons in a van der Waals crystal. <i>Nanomaterials</i>. 2021;11(1). doi:<a href=\"https://doi.org/10.3390/nano11010120\">10.3390/nano11010120</a>","ista":"Aguilar-Merino P, Álvarez-Pérez G, Taboada-Gutiérrez J, Duan J, Prieto Gonzalez I, Álvarez-Prado LM, Nikitin AY, Martín-Sánchez J, Alonso-González P. 2021. Extracting the infrared permittivity of SiO2 substrates locally by near-field imaging of phonon polaritons in a van der Waals crystal. Nanomaterials. 11(1), 120.","ieee":"P. Aguilar-Merino <i>et al.</i>, “Extracting the infrared permittivity of SiO2 substrates locally by near-field imaging of phonon polaritons in a van der Waals crystal,” <i>Nanomaterials</i>, vol. 11, no. 1. MDPI, 2021.","apa":"Aguilar-Merino, P., Álvarez-Pérez, G., Taboada-Gutiérrez, J., Duan, J., Prieto Gonzalez, I., Álvarez-Prado, L. M., … Alonso-González, P. (2021). Extracting the infrared permittivity of SiO2 substrates locally by near-field imaging of phonon polaritons in a van der Waals crystal. <i>Nanomaterials</i>. MDPI. <a href=\"https://doi.org/10.3390/nano11010120\">https://doi.org/10.3390/nano11010120</a>","chicago":"Aguilar-Merino, Patricia, Gonzalo Álvarez-Pérez, Javier Taboada-Gutiérrez, Jiahua Duan, Ivan Prieto Gonzalez, Luis Manuel Álvarez-Prado, Alexey Y. Nikitin, Javier Martín-Sánchez, and Pablo Alonso-González. “Extracting the Infrared Permittivity of SiO2 Substrates Locally by Near-Field Imaging of Phonon Polaritons in a van Der Waals Crystal.” <i>Nanomaterials</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/nano11010120\">https://doi.org/10.3390/nano11010120</a>.","short":"P. Aguilar-Merino, G. Álvarez-Pérez, J. Taboada-Gutiérrez, J. Duan, I. Prieto Gonzalez, L.M. Álvarez-Prado, A.Y. Nikitin, J. Martín-Sánchez, P. Alonso-González, Nanomaterials 11 (2021).","mla":"Aguilar-Merino, Patricia, et al. “Extracting the Infrared Permittivity of SiO2 Substrates Locally by Near-Field Imaging of Phonon Polaritons in a van Der Waals Crystal.” <i>Nanomaterials</i>, vol. 11, no. 1, 120, MDPI, 2021, doi:<a href=\"https://doi.org/10.3390/nano11010120\">10.3390/nano11010120</a>."},"year":"2021","publisher":"MDPI","day":"07","isi":1,"oa_version":"Published Version","status":"public","_id":"9038","article_number":"120","doi":"10.3390/nano11010120","type":"journal_article","pmid":1,"publication_identifier":{"eissn":["2079-4991"]},"title":"Extracting the infrared permittivity of SiO2 substrates locally by near-field imaging of phonon polaritons in a van der Waals crystal","oa":1,"author":[{"full_name":"Aguilar-Merino, Patricia","last_name":"Aguilar-Merino","first_name":"Patricia"},{"first_name":"Gonzalo","last_name":"Álvarez-Pérez","full_name":"Álvarez-Pérez, Gonzalo"},{"first_name":"Javier","full_name":"Taboada-Gutiérrez, Javier","last_name":"Taboada-Gutiérrez"},{"full_name":"Duan, Jiahua","last_name":"Duan","first_name":"Jiahua"},{"first_name":"Ivan","orcid":"0000-0002-7370-5357","id":"2A307FE2-F248-11E8-B48F-1D18A9856A87","last_name":"Prieto Gonzalez","full_name":"Prieto Gonzalez, Ivan"},{"full_name":"Álvarez-Prado, Luis Manuel","last_name":"Álvarez-Prado","first_name":"Luis Manuel"},{"last_name":"Nikitin","full_name":"Nikitin, Alexey Y.","first_name":"Alexey Y."},{"full_name":"Martín-Sánchez, Javier","last_name":"Martín-Sánchez","first_name":"Javier"},{"last_name":"Alonso-González","full_name":"Alonso-González, Pablo","first_name":"Pablo"}],"publication":"Nanomaterials","scopus_import":"1","quality_controlled":"1","date_created":"2021-01-24T23:01:09Z","article_processing_charge":"No","month":"01","department":[{"_id":"NanoFab"}],"volume":11,"language":[{"iso":"eng"}],"publication_status":"published"},{"external_id":{"isi":["000627134400001"],"pmid":["33717158"]},"intvolume":"        12","file":[{"relation":"main_file","checksum":"663f5a48375e42afa4bfef58d42ec186","access_level":"open_access","creator":"dernst","date_updated":"2021-03-22T12:08:26Z","content_type":"application/pdf","file_id":"9277","success":1,"file_size":3740146,"date_created":"2021-03-22T12:08:26Z","file_name":"2021_FrontiersImmumo_Vaahtomeri.pdf"}],"isi":1,"day":"25","publisher":"Frontiers","citation":{"chicago":"Vaahtomeri, Kari, Christine Moussion, Robert Hauschild, and Michael K Sixt. “Shape and Function of Interstitial Chemokine CCL21 Gradients Are Independent of Heparan Sulfates Produced by Lymphatic Endothelium.” <i>Frontiers in Immunology</i>. Frontiers, 2021. <a href=\"https://doi.org/10.3389/fimmu.2021.630002\">https://doi.org/10.3389/fimmu.2021.630002</a>.","mla":"Vaahtomeri, Kari, et al. “Shape and Function of Interstitial Chemokine CCL21 Gradients Are Independent of Heparan Sulfates Produced by Lymphatic Endothelium.” <i>Frontiers in Immunology</i>, vol. 12, 630002, Frontiers, 2021, doi:<a href=\"https://doi.org/10.3389/fimmu.2021.630002\">10.3389/fimmu.2021.630002</a>.","short":"K. Vaahtomeri, C. Moussion, R. Hauschild, M.K. Sixt, Frontiers in Immunology 12 (2021).","ista":"Vaahtomeri K, Moussion C, Hauschild R, Sixt MK. 2021. Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium. Frontiers in Immunology. 12, 630002.","ama":"Vaahtomeri K, Moussion C, Hauschild R, Sixt MK. Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium. <i>Frontiers in Immunology</i>. 2021;12. doi:<a href=\"https://doi.org/10.3389/fimmu.2021.630002\">10.3389/fimmu.2021.630002</a>","apa":"Vaahtomeri, K., Moussion, C., Hauschild, R., &#38; Sixt, M. K. (2021). Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium. <i>Frontiers in Immunology</i>. Frontiers. <a href=\"https://doi.org/10.3389/fimmu.2021.630002\">https://doi.org/10.3389/fimmu.2021.630002</a>","ieee":"K. Vaahtomeri, C. Moussion, R. Hauschild, and M. K. Sixt, “Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium,” <i>Frontiers in Immunology</i>, vol. 12. Frontiers, 2021."},"acknowledgement":"This work was supported by Sigrid Juselius fellowship (KV), University of Helsinki 3-year research grant (KV), Academy of Finland Research fellow funding (315710, to KV), the European Research Council (ERC CoG 724373 to MS), and by the Austrian Science foundation (FWF) (Y564-B12 START award to MS).\r\nTaija Mäkinen is acknowledged for providing Prox1CreERT2 transgenic mice and Yu Yamaguchi for providing the conditional Ext1 mouse strain.","year":"2021","status":"public","_id":"9259","oa_version":"Published Version","has_accepted_license":"1","date_published":"2021-02-25T00:00:00Z","ddc":["570"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.3389/fimmu.2021.630002","article_type":"original","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","ec_funded":1,"file_date_updated":"2021-03-22T12:08:26Z","abstract":[{"text":"Gradients of chemokines and growth factors guide migrating cells and morphogenetic processes. Migration of antigen-presenting dendritic cells from the interstitium into the lymphatic system is dependent on chemokine CCL21, which is secreted by endothelial cells of the lymphatic capillary, binds heparan sulfates and forms gradients decaying into the interstitium. Despite the importance of CCL21 gradients, and chemokine gradients in general, the mechanisms of gradient formation are unclear. Studies on fibroblast growth factors have shown that limited diffusion is crucial for gradient formation. Here, we used the mouse dermis as a model tissue to address the necessity of CCL21 anchoring to lymphatic capillary heparan sulfates in the formation of interstitial CCL21 gradients. Surprisingly, the absence of lymphatic endothelial heparan sulfates resulted only in a modest decrease of CCL21 levels at the lymphatic capillaries and did neither affect interstitial CCL21 gradient shape nor dendritic cell migration toward lymphatic capillaries. Thus, heparan sulfates at the level of the lymphatic endothelium are dispensable for the formation of a functional CCL21 gradient.","lang":"eng"}],"date_updated":"2025-04-14T07:42:07Z","project":[{"grant_number":"724373","call_identifier":"H2020","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients"},{"grant_number":"Y 564-B12","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"quality_controlled":"1","article_processing_charge":"No","date_created":"2021-03-21T23:01:20Z","month":"02","publication_status":"published","volume":12,"language":[{"iso":"eng"}],"department":[{"_id":"MiSi"},{"_id":"Bio"}],"article_number":"630002","title":"Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium","oa":1,"pmid":1,"publication_identifier":{"eissn":["1664-3224"]},"type":"journal_article","doi":"10.3389/fimmu.2021.630002","publication":"Frontiers in Immunology","author":[{"full_name":"Vaahtomeri, Kari","last_name":"Vaahtomeri","id":"368EE576-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7829-3518","first_name":"Kari"},{"first_name":"Christine","last_name":"Moussion","id":"3356F664-F248-11E8-B48F-1D18A9856A87","full_name":"Moussion, Christine"},{"orcid":"0000-0001-9843-3522","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert"},{"first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"corr_author":"1","scopus_import":"1"},{"file_date_updated":"2021-03-22T12:49:00Z","abstract":[{"text":"Sequence-specific oligomers with predictable folding patterns, i.e., foldamers, provide new opportunities to mimic α-helical peptides and design inhibitors of protein-protein interactions. One major hurdle of this strategy is to retain the correct orientation of key side chains involved in protein surface recognition. Here, we show that the structural plasticity of a foldamer backbone may notably contribute to the required spatial adjustment for optimal interaction with the protein surface. By using oligoureas as α helix mimics, we designed a foldamer/peptide hybrid inhibitor of histone chaperone ASF1, a key regulator of chromatin dynamics. The crystal structure of its complex with ASF1 reveals a notable plasticity of the urea backbone, which adapts to the ASF1 surface to maintain the same binding interface. One additional benefit of generating ASF1 ligands with nonpeptide oligourea segments is the resistance to proteolysis in human plasma, which was highly improved compared to the cognate α-helical peptide.","lang":"eng"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2023-08-07T14:20:26Z","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png"},"fulldoi":"https://doi.org/10.1126/sciadv.abd9153","article_type":"original","date_published":"2021-03-19T00:00:00Z","has_accepted_license":"1","ddc":["570"],"publisher":"American Association for the Advancement of Science","acknowledgement":"We thank the Synchrotron SOLEIL, the European Synchrotron Radiation Facility (ESRF), and the French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INBS-05. We are particularly grateful to A. Clavier and A. Campalans for help in setting up and performing the cell penetration assays. Funding: Research was funded by the French Centre National de Recherche Scientifique (CNRS), the Commissariat à l’Energie Atomique (CEA), University of Bordeaux, University Paris-Saclay, and the Synchrotron Soleil. The project was supported by the ANR 2007 BREAKABOUND (JC-07-216078), 2011 BIPBIP (ANR-10-BINF-0003), 2012 CHAPINHIB (ANR-12-BSV5-0022-01), 2015 CHIPSET (ANR-15-CE11-008-01), 2015 HIMPP2I (ANR-15-CE07-0010), and the program labeled by the ARC foundation 2016 PGA1*20160203953). M.B. was supported by Canceropole (Paris, France) and a grant for young researchers from La Ligue contre le Cancer. J.M. was supported by La Ligue contre le Cancer.","year":"2021","citation":{"short":"J. Mbianda, M.M. Bakail, C. André, G. Moal, M.E. Perrin, G. Pinna, R. Guerois, F. Becher, P. Legrand, S. Traoré, C. Douat, G. Guichard, F. Ochsenbein, Science Advances 7 (2021).","mla":"Mbianda, Johanne, et al. “Optimal Anchoring of a Foldamer Inhibitor of ASF1 Histone Chaperone through Backbone Plasticity.” <i>Science Advances</i>, vol. 7, no. 12, eabd9153, American Association for the Advancement of Science, 2021, doi:<a href=\"https://doi.org/10.1126/sciadv.abd9153\">10.1126/sciadv.abd9153</a>.","chicago":"Mbianda, Johanne, May M Bakail, Christophe André, Gwenaëlle Moal, Marie E. Perrin, Guillaume Pinna, Raphaël Guerois, et al. “Optimal Anchoring of a Foldamer Inhibitor of ASF1 Histone Chaperone through Backbone Plasticity.” <i>Science Advances</i>. American Association for the Advancement of Science, 2021. <a href=\"https://doi.org/10.1126/sciadv.abd9153\">https://doi.org/10.1126/sciadv.abd9153</a>.","apa":"Mbianda, J., Bakail, M. M., André, C., Moal, G., Perrin, M. E., Pinna, G., … Ochsenbein, F. (2021). Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.abd9153\">https://doi.org/10.1126/sciadv.abd9153</a>","ieee":"J. Mbianda <i>et al.</i>, “Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity,” <i>Science Advances</i>, vol. 7, no. 12. American Association for the Advancement of Science, 2021.","ama":"Mbianda J, Bakail MM, André C, et al. Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity. <i>Science Advances</i>. 2021;7(12). doi:<a href=\"https://doi.org/10.1126/sciadv.abd9153\">10.1126/sciadv.abd9153</a>","ista":"Mbianda J, Bakail MM, André C, Moal G, Perrin ME, Pinna G, Guerois R, Becher F, Legrand P, Traoré S, Douat C, Guichard G, Ochsenbein F. 2021. Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity. Science Advances. 7(12), eabd9153."},"isi":1,"day":"19","oa_version":"Published Version","_id":"9262","status":"public","intvolume":"         7","file":[{"access_level":"open_access","checksum":"737624cd0e630ffa7c52797a690500e3","relation":"main_file","creator":"dernst","content_type":"application/pdf","date_updated":"2021-03-22T12:49:00Z","success":1,"file_id":"9280","file_size":837156,"date_created":"2021-03-22T12:49:00Z","file_name":"2021_ScienceAdv_Mbianda.pdf"}],"issue":"12","external_id":{"isi":["000633443000011"],"pmid":["33741589"]},"author":[{"first_name":"Johanne","last_name":"Mbianda","full_name":"Mbianda, Johanne"},{"orcid":"0000-0002-9592-1587","first_name":"May M","full_name":"Bakail, May M","id":"FB3C3F8E-522F-11EA-B186-22963DDC885E","last_name":"Bakail"},{"first_name":"Christophe","full_name":"André, Christophe","last_name":"André"},{"first_name":"Gwenaëlle","last_name":"Moal","full_name":"Moal, Gwenaëlle"},{"first_name":"Marie E.","full_name":"Perrin, Marie E.","last_name":"Perrin"},{"first_name":"Guillaume","full_name":"Pinna, Guillaume","last_name":"Pinna"},{"first_name":"Raphaël","full_name":"Guerois, Raphaël","last_name":"Guerois"},{"full_name":"Becher, Francois","last_name":"Becher","first_name":"Francois"},{"last_name":"Legrand","full_name":"Legrand, Pierre","first_name":"Pierre"},{"last_name":"Traoré","full_name":"Traoré, Seydou","first_name":"Seydou"},{"first_name":"Céline","full_name":"Douat, Céline","last_name":"Douat"},{"first_name":"Gilles","full_name":"Guichard, Gilles","last_name":"Guichard"},{"last_name":"Ochsenbein","full_name":"Ochsenbein, Françoise","first_name":"Françoise"}],"publication":"Science Advances","article_number":"eabd9153","type":"journal_article","doi":"10.1126/sciadv.abd9153","oa":1,"title":"Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity","pmid":1,"publication_identifier":{"issn":["2375-2548"]},"month":"03","publication_status":"published","volume":7,"language":[{"iso":"eng"}],"department":[{"_id":"CampIT"}],"quality_controlled":"1","article_processing_charge":"No","date_created":"2021-03-22T07:14:03Z"},{"author":[{"first_name":"Christian","full_name":"Prehal, Christian","last_name":"Prehal"},{"last_name":"Samojlov","full_name":"Samojlov, Aleksej","first_name":"Aleksej"},{"first_name":"Manfred","last_name":"Nachtnebel","full_name":"Nachtnebel, Manfred"},{"full_name":"Lovicar, Ludek","last_name":"Lovicar","id":"36DB3A20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6206-4200","first_name":"Ludek"},{"first_name":"Manfred","full_name":"Kriechbaum, Manfred","last_name":"Kriechbaum"},{"last_name":"Amenitsch","full_name":"Amenitsch, Heinz","first_name":"Heinz"},{"first_name":"Stefan Alexander","orcid":"0000-0003-2902-5319","full_name":"Freunberger, Stefan Alexander","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425","last_name":"Freunberger"}],"publication":"Proceedings of the National Academy of Sciences of the United States of America","scopus_import":"1","article_number":"e2021893118","type":"journal_article","doi":"10.1073/pnas.2021893118","main_file_link":[{"open_access":"1","url":"https://doi.org/10.26434/chemrxiv.11447775"}],"oa":1,"title":"In situ small-angle X-ray scattering reveals solution phase discharge of Li–O2 batteries with weakly solvating electrolytes","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"pmid":1,"month":"04","publication_status":"published","volume":118,"department":[{"_id":"StFr"},{"_id":"EM-Fac"}],"language":[{"iso":"eng"}],"quality_controlled":"1","article_processing_charge":"No","date_created":"2021-03-31T07:00:01Z","abstract":[{"text":"Electrodepositing insulating lithium peroxide (Li2O2) is the key process during discharge of aprotic Li–O2 batteries and determines rate, capacity, and reversibility. Current understanding states that the partition between surface adsorbed and dissolved lithium superoxide governs whether Li2O2 grows as a conformal surface film or larger particles, leading to low or high capacities, respectively. However, better understanding governing factors for Li2O2 packing density and capacity requires structural sensitive in situ metrologies. Here, we establish in situ small- and wide-angle X-ray scattering (SAXS/WAXS) as a suitable method to record the Li2O2 phase evolution with atomic to submicrometer resolution during cycling a custom-built in situ Li–O2 cell. Combined with sophisticated data analysis, SAXS allows retrieving rich quantitative structural information from complex multiphase systems. Surprisingly, we find that features are absent that would point at a Li2O2 surface film formed via two consecutive electron transfers, even in poorly solvating electrolytes thought to be prototypical for surface growth. All scattering data can be modeled by stacks of thin Li2O2 platelets potentially forming large toroidal particles. Li2O2 solution growth is further justified by rotating ring-disk electrode measurements and electron microscopy. Higher discharge overpotentials lead to smaller Li2O2 particles, but there is no transition to an electronically passivating, conformal Li2O2 coating. Hence, mass transport of reactive species rather than electronic transport through a Li2O2 film limits the discharge capacity. Provided that species mobilities and carbon surface areas are high, this allows for high discharge capacities even in weakly solvating electrolytes. The currently accepted Li–O2 reaction mechanism ought to be reconsidered.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2025-06-12T06:56:39Z","keyword":["small-angle X-ray scattering","oxygen reduction","disproportionation","Li-air battery"],"fulldoi":"https://doi.org/10.1073/pnas.2021893118","article_type":"original","date_published":"2021-04-06T00:00:00Z","publisher":"National Academy of Sciences","year":"2021","acknowledgement":"S.A.F. and C.P. are indebted to the European Research Council under the European Union's Horizon 2020 research and innovation program (Grant Agreement No. 636069), the Austrian Federal Ministry of Science, Research and Economy, and the Austrian Research Promotion Agency (Grant No. 845364). We acknowledge A. Zankel and H. Schroettner for support with SEM measurements. C.P. thanks N. Kostoglou, C. Koczwara, M. Hartmann, and M. Burian for discussions on gas sorption analysis, C++ programming, Monte Carlo modeling, and in situ SAXS experiments, respectively. We thank S. Stadlbauer for help with Karl Fischer titration, R. Riccò for gas sorption measurements, and acknowledge Graz University of Technology for support through the Lead Project LP-03. Likewise, the use of SOMAPP Lab, a core facility supported by the Austrian Federal Ministry of Education, Science and Research, the Graz University of Technology, the University of Graz, and Anton Paar GmbH is acknowledged. S.A.F. is indebted to Institute of Science and Technology Austria (IST Austria) for support. This research was supported by the Scientific Service Units of IST Austria through resources provided by the Electron Microscopy Facility.","citation":{"ama":"Prehal C, Samojlov A, Nachtnebel M, et al. In situ small-angle X-ray scattering reveals solution phase discharge of Li–O2 batteries with weakly solvating electrolytes. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2021;118(14). doi:<a href=\"https://doi.org/10.1073/pnas.2021893118\">10.1073/pnas.2021893118</a>","ista":"Prehal C, Samojlov A, Nachtnebel M, Lovicar L, Kriechbaum M, Amenitsch H, Freunberger SA. 2021. In situ small-angle X-ray scattering reveals solution phase discharge of Li–O2 batteries with weakly solvating electrolytes. Proceedings of the National Academy of Sciences of the United States of America. 118(14), e2021893118.","ieee":"C. Prehal <i>et al.</i>, “In situ small-angle X-ray scattering reveals solution phase discharge of Li–O2 batteries with weakly solvating electrolytes,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 14. National Academy of Sciences, 2021.","apa":"Prehal, C., Samojlov, A., Nachtnebel, M., Lovicar, L., Kriechbaum, M., Amenitsch, H., &#38; Freunberger, S. A. (2021). In situ small-angle X-ray scattering reveals solution phase discharge of Li–O2 batteries with weakly solvating electrolytes. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2021893118\">https://doi.org/10.1073/pnas.2021893118</a>","chicago":"Prehal, Christian, Aleksej Samojlov, Manfred Nachtnebel, Ludek Lovicar, Manfred Kriechbaum, Heinz Amenitsch, and Stefan Alexander Freunberger. “In Situ Small-Angle X-Ray Scattering Reveals Solution Phase Discharge of Li–O2 Batteries with Weakly Solvating Electrolytes.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2021. <a href=\"https://doi.org/10.1073/pnas.2021893118\">https://doi.org/10.1073/pnas.2021893118</a>.","short":"C. Prehal, A. Samojlov, M. Nachtnebel, L. Lovicar, M. Kriechbaum, H. Amenitsch, S.A. Freunberger, Proceedings of the National Academy of Sciences of the United States of America 118 (2021).","mla":"Prehal, Christian, et al. “In Situ Small-Angle X-Ray Scattering Reveals Solution Phase Discharge of Li–O2 Batteries with Weakly Solvating Electrolytes.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 14, e2021893118, National Academy of Sciences, 2021, doi:<a href=\"https://doi.org/10.1073/pnas.2021893118\">10.1073/pnas.2021893118</a>."},"isi":1,"day":"06","oa_version":"Preprint","_id":"9301","status":"public","intvolume":"       118","issue":"14","external_id":{"pmid":["33785597"],"isi":["000637398300050"]},"acknowledged_ssus":[{"_id":"EM-Fac"}]},{"issue":"6","external_id":{"pmid":["33711356"],"isi":["000661088500005"]},"intvolume":"       357","file":[{"file_id":"9339","success":1,"file_name":"2021_JourNeuroscienceMeth_Zhang.pdf","date_created":"2021-04-19T08:30:22Z","file_size":6924738,"relation":"main_file","access_level":"open_access","checksum":"2a5800d91b96d08b525e17319dcd5e44","content_type":"application/pdf","date_updated":"2021-04-19T08:30:22Z","creator":"dernst"}],"acknowledged_ssus":[{"_id":"SSU"}],"isi":1,"day":"09","publisher":"Elsevier","citation":{"apa":"Zhang, X., Schlögl, A., Vandael, D. H., &#38; Jonas, P. M. (2021). MOD: A novel machine-learning optimal-filtering method for accurate and efficient detection of subthreshold synaptic events in vivo. <i>Journal of Neuroscience Methods</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jneumeth.2021.109125\">https://doi.org/10.1016/j.jneumeth.2021.109125</a>","ieee":"X. Zhang, A. Schlögl, D. H. Vandael, and P. M. Jonas, “MOD: A novel machine-learning optimal-filtering method for accurate and efficient detection of subthreshold synaptic events in vivo,” <i>Journal of Neuroscience Methods</i>, vol. 357, no. 6. Elsevier, 2021.","ama":"Zhang X, Schlögl A, Vandael DH, Jonas PM. MOD: A novel machine-learning optimal-filtering method for accurate and efficient detection of subthreshold synaptic events in vivo. <i>Journal of Neuroscience Methods</i>. 2021;357(6). doi:<a href=\"https://doi.org/10.1016/j.jneumeth.2021.109125\">10.1016/j.jneumeth.2021.109125</a>","ista":"Zhang X, Schlögl A, Vandael DH, Jonas PM. 2021. MOD: A novel machine-learning optimal-filtering method for accurate and efficient detection of subthreshold synaptic events in vivo. Journal of Neuroscience Methods. 357(6), 109125.","mla":"Zhang, Xiaomin, et al. “MOD: A Novel Machine-Learning Optimal-Filtering Method for Accurate and Efficient Detection of Subthreshold Synaptic Events in Vivo.” <i>Journal of Neuroscience Methods</i>, vol. 357, no. 6, 109125, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/j.jneumeth.2021.109125\">10.1016/j.jneumeth.2021.109125</a>.","short":"X. Zhang, A. Schlögl, D.H. Vandael, P.M. Jonas, Journal of Neuroscience Methods 357 (2021).","chicago":"Zhang, Xiaomin, Alois Schlögl, David H Vandael, and Peter M Jonas. “MOD: A Novel Machine-Learning Optimal-Filtering Method for Accurate and Efficient Detection of Subthreshold Synaptic Events in Vivo.” <i>Journal of Neuroscience Methods</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.jneumeth.2021.109125\">https://doi.org/10.1016/j.jneumeth.2021.109125</a>."},"year":"2021","acknowledgement":"This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement number 692692 to P.J.) and the Fond zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award to P.J.). We thank Drs. Jozsef Csicsvari, Christoph Lampert, and Federico Stella for critically reading previous manuscript versions. We are also grateful to Drs. Josh Merel and Ben Shababo for their help with applying the Bayesian detection method to our data. We also thank Florian Marr for technical assistance, Eleftheria Kralli-Beller for manuscript editing, and the Scientific Service Units of IST Austria for efficient support.","status":"public","_id":"9329","oa_version":"Published Version","date_published":"2021-03-09T00:00:00Z","has_accepted_license":"1","ddc":["570"],"tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"fulldoi":"https://doi.org/10.1016/j.jneumeth.2021.109125","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2021-04-19T08:30:22Z","abstract":[{"text":"Background: To understand information coding in single neurons, it is necessary to analyze subthreshold synaptic events, action potentials (APs), and their interrelation in different behavioral states. However, detecting excitatory postsynaptic potentials (EPSPs) or currents (EPSCs) in behaving animals remains challenging, because of unfavorable signal-to-noise ratio, high frequency, fluctuating amplitude, and variable time course of synaptic events.\r\nNew method: We developed a method for synaptic event detection, termed MOD (Machine-learning Optimal-filtering Detection-procedure), which combines concepts of supervised machine learning and optimal Wiener filtering. Experts were asked to manually score short epochs of data. The algorithm was trained to obtain the optimal filter coefficients of a Wiener filter and the optimal detection threshold. Scored and unscored data were then processed with the optimal filter, and events were detected as peaks above threshold.\r\nResults: We challenged MOD with EPSP traces in vivo in mice during spatial navigation and EPSC traces in vitro in slices under conditions of enhanced transmitter release. The area under the curve (AUC) of the receiver operating characteristics (ROC) curve was, on average, 0.894 for in vivo and 0.969 for in vitro data sets, indicating high detection accuracy and efficiency.\r\nComparison with existing methods: When benchmarked using a (1 − AUC)−1 metric, MOD outperformed previous methods (template-fit, deconvolution, and Bayesian methods) by an average factor of 3.13 for in vivo data sets, but showed comparable (template-fit, deconvolution) or higher (Bayesian) computational efficacy.\r\nConclusions: MOD may become an important new tool for large-scale, real-time analysis of synaptic activity.","lang":"eng"}],"ec_funded":1,"date_updated":"2025-06-12T06:39:15Z","project":[{"grant_number":"692692","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","call_identifier":"H2020"},{"_id":"25C5A090-B435-11E9-9278-68D0E5697425","name":"Synaptic communication in neuronal microcircuits","call_identifier":"FWF","grant_number":"Z00312"}],"quality_controlled":"1","article_processing_charge":"Yes (via OA deal)","date_created":"2021-04-18T22:01:39Z","month":"03","publication_status":"published","department":[{"_id":"PeJo"},{"_id":"ScienComp"}],"language":[{"iso":"eng"}],"volume":357,"article_number":"109125","oa":1,"title":"MOD: A novel machine-learning optimal-filtering method for accurate and efficient detection of subthreshold synaptic events in vivo","publication_identifier":{"eissn":["1872-678X"],"issn":["0165-0270"]},"pmid":1,"type":"journal_article","doi":"10.1016/j.jneumeth.2021.109125","publication":"Journal of Neuroscience Methods","author":[{"first_name":"Xiaomin","full_name":"Zhang, Xiaomin","last_name":"Zhang","id":"423EC9C2-F248-11E8-B48F-1D18A9856A87"},{"id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl","full_name":"Schlögl, Alois","first_name":"Alois","orcid":"0000-0002-5621-8100"},{"orcid":"0000-0001-7577-1676","first_name":"David H","full_name":"Vandael, David H","last_name":"Vandael","id":"3AE48E0A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Jonas, Peter M","first_name":"Peter M","orcid":"0000-0001-5001-4804"}],"scopus_import":"1"},{"date_published":"2021-04-06T00:00:00Z","has_accepted_license":"1","ddc":["570"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1073/pnas.1920827118","article_type":"original","date_updated":"2025-06-12T06:56:21Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"In nerve cells the genes encoding for α2δ subunits of voltage-gated calcium channels have been linked to synaptic functions and neurological disease. Here we show that α2δ subunits are essential for the formation and organization of glutamatergic synapses. Using a cellular α2δ subunit triple-knockout/knockdown model, we demonstrate a failure in presynaptic differentiation evidenced by defective presynaptic calcium channel clustering and calcium influx, smaller presynaptic active zones, and a strongly reduced accumulation of presynaptic vesicle-associated proteins (synapsin and vGLUT). The presynaptic defect is associated with the downscaling of postsynaptic AMPA receptors and the postsynaptic density. The role of α2δ isoforms as synaptic organizers is highly redundant, as each individual α2δ isoform can rescue presynaptic calcium channel trafficking and expression of synaptic proteins. Moreover, α2δ-2 and α2δ-3 with mutated metal ion-dependent adhesion sites can fully rescue presynaptic synapsin expression but only partially calcium channel trafficking, suggesting that the regulatory role of α2δ subunits is independent from its role as a calcium channel subunit. Our findings influence the current view on excitatory synapse formation. First, our study suggests that postsynaptic differentiation is secondary to presynaptic differentiation. Second, the dependence of presynaptic differentiation on α2δ implicates α2δ subunits as potential nucleation points for the organization of synapses. Finally, our results suggest that α2δ subunits act as transsynaptic organizers of glutamatergic synapses, thereby aligning the synaptic active zone with the postsynaptic density."}],"file_date_updated":"2021-04-19T10:10:56Z","ec_funded":1,"acknowledged_ssus":[{"_id":"EM-Fac"}],"issue":"14","external_id":{"isi":["000637398300002"],"pmid":["33782113"]},"intvolume":"       118","file":[{"date_created":"2021-04-19T10:10:56Z","file_size":2603911,"file_name":"2021_PNAS_Schoepf.pdf","success":1,"file_id":"9340","creator":"dernst","content_type":"application/pdf","date_updated":"2021-04-19T10:10:56Z","checksum":"dd014f68ae9d7d8d8fc4139a24e04506","access_level":"open_access","relation":"main_file"}],"_id":"9330","status":"public","oa_version":"Published Version","isi":1,"day":"06","publisher":"National Academy of Sciences","year":"2021","acknowledgement":"We thank Arnold Schwartz for providing α2δ-1 knockout mice; Ariane Benedetti, Sabine Baumgartner, Sandra Demetz, and Irene Mahlknecht for technical support; Nadine Ortner and Andreas Lieb for electrophysiological experiments; the team of the Electron Microscopy Facility at the Institute of Science and Technology Austria for technical support related to ultrastructural analysis; Hermann Dietrich and Anja Beierfuß and her team for animal care; Jutta Engel and Jörg Striessnig for critical discussions; and Bruno Benedetti and Bernhard Flucher for critical discussions and reading the manuscript. This study was supported by Austrian Science Fund Grants P24079, F44060, F44150, and DOC30-B30 (to G.J.O.) and T855 (to M.C.), European Research Council Grant AdG 694539 (to R.S.), Deutsche Forschungsgemeinschaft\r\nGrant SFB1348-TP A03 (to M.M.), and Interdisziplinäre Zentrum für Klinische Forschung Münster Grant Mi3/004/19 (to M.M.). This work is part of the PhD theses of C.L.S., S.M.G., and C.A.","citation":{"short":"C.L. Schöpf, C. Ablinger, S.M. Geisler, R.I. Stanika, M. Campiglio, W. Kaufmann, B. Nimmervoll, B. Schlick, J. Brockhaus, M. Missler, R. Shigemoto, G.J. Obermair, Proceedings of the National Academy of Sciences of the United States of America 118 (2021).","mla":"Schöpf, Clemens L., et al. “Presynaptic Α2δ Subunits Are Key Organizers of Glutamatergic Synapses.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 14, National Academy of Sciences, 2021, doi:<a href=\"https://doi.org/10.1073/pnas.1920827118\">10.1073/pnas.1920827118</a>.","chicago":"Schöpf, Clemens L., Cornelia Ablinger, Stefanie M. Geisler, Ruslan I. Stanika, Marta Campiglio, Walter Kaufmann, Benedikt Nimmervoll, et al. “Presynaptic Α2δ Subunits Are Key Organizers of Glutamatergic Synapses.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2021. <a href=\"https://doi.org/10.1073/pnas.1920827118\">https://doi.org/10.1073/pnas.1920827118</a>.","apa":"Schöpf, C. L., Ablinger, C., Geisler, S. M., Stanika, R. I., Campiglio, M., Kaufmann, W., … Obermair, G. J. (2021). Presynaptic α2δ subunits are key organizers of glutamatergic synapses. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1920827118\">https://doi.org/10.1073/pnas.1920827118</a>","ieee":"C. L. Schöpf <i>et al.</i>, “Presynaptic α2δ subunits are key organizers of glutamatergic synapses,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 14. National Academy of Sciences, 2021.","ama":"Schöpf CL, Ablinger C, Geisler SM, et al. Presynaptic α2δ subunits are key organizers of glutamatergic synapses. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2021;118(14). doi:<a href=\"https://doi.org/10.1073/pnas.1920827118\">10.1073/pnas.1920827118</a>","ista":"Schöpf CL, Ablinger C, Geisler SM, Stanika RI, Campiglio M, Kaufmann W, Nimmervoll B, Schlick B, Brockhaus J, Missler M, Shigemoto R, Obermair GJ. 2021. Presynaptic α2δ subunits are key organizers of glutamatergic synapses. Proceedings of the National Academy of Sciences of the United States of America. 118(14)."},"oa":1,"title":"Presynaptic α2δ subunits are key organizers of glutamatergic synapses","publication_identifier":{"eissn":["1091-6490"]},"pmid":1,"type":"journal_article","doi":"10.1073/pnas.1920827118","scopus_import":"1","publication":"Proceedings of the National Academy of Sciences of the United States of America","author":[{"first_name":"Clemens L.","full_name":"Schöpf, Clemens L.","last_name":"Schöpf"},{"first_name":"Cornelia","last_name":"Ablinger","full_name":"Ablinger, Cornelia"},{"first_name":"Stefanie M.","last_name":"Geisler","full_name":"Geisler, Stefanie M."},{"last_name":"Stanika","full_name":"Stanika, Ruslan I.","first_name":"Ruslan I."},{"full_name":"Campiglio, Marta","last_name":"Campiglio","first_name":"Marta"},{"last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","first_name":"Walter"},{"first_name":"Benedikt","last_name":"Nimmervoll","full_name":"Nimmervoll, Benedikt"},{"last_name":"Schlick","full_name":"Schlick, Bettina","first_name":"Bettina"},{"full_name":"Brockhaus, Johannes","last_name":"Brockhaus","first_name":"Johannes"},{"first_name":"Markus","full_name":"Missler, Markus","last_name":"Missler"},{"orcid":"0000-0001-8761-9444","first_name":"Ryuichi","full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto"},{"first_name":"Gerald J.","full_name":"Obermair, Gerald J.","last_name":"Obermair"}],"article_processing_charge":"No","date_created":"2021-04-18T22:01:40Z","project":[{"grant_number":"694539","_id":"25CA28EA-B435-11E9-9278-68D0E5697425","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour","call_identifier":"H2020"}],"quality_controlled":"1","publication_status":"published","volume":118,"department":[{"_id":"EM-Fac"},{"_id":"RySh"}],"language":[{"iso":"eng"}],"month":"04"},{"publication":"Science Advances","author":[{"first_name":"J.","full_name":"Duan, J.","last_name":"Duan"},{"last_name":"Álvarez-Pérez","full_name":"Álvarez-Pérez, G.","first_name":"G."},{"full_name":"Voronin, K. V.","last_name":"Voronin","first_name":"K. V."},{"orcid":"0000-0002-7370-5357","first_name":"Ivan","full_name":"Prieto Gonzalez, Ivan","last_name":"Prieto Gonzalez","id":"2A307FE2-F248-11E8-B48F-1D18A9856A87"},{"first_name":"J.","full_name":"Taboada-Gutiérrez, J.","last_name":"Taboada-Gutiérrez"},{"last_name":"Volkov","full_name":"Volkov, V. S.","first_name":"V. S."},{"full_name":"Martín-Sánchez, J.","last_name":"Martín-Sánchez","first_name":"J."},{"first_name":"A. Y.","full_name":"Nikitin, A. Y.","last_name":"Nikitin"},{"first_name":"P.","last_name":"Alonso-González","full_name":"Alonso-González, P."}],"scopus_import":"1","article_number":"eabf2690","pmid":1,"publication_identifier":{"eissn":["2375-2548"]},"oa":1,"title":"Enabling propagation of anisotropic polaritons along forbidden directions via a topological transition","doi":"10.1126/sciadv.abf2690","type":"journal_article","month":"04","volume":7,"department":[{"_id":"NanoFab"}],"language":[{"iso":"eng"}],"publication_status":"published","quality_controlled":"1","date_created":"2021-04-18T22:01:42Z","article_processing_charge":"No","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","abstract":[{"text":"Polaritons with directional in-plane propagation and ultralow losses in van der Waals (vdW) crystals promise unprecedented manipulation of light at the nanoscale. However, these polaritons present a crucial limitation: their directional propagation is intrinsically determined by the crystal structure of the host material, imposing forbidden directions of propagation. Here, we demonstrate that directional polaritons (in-plane hyperbolic phonon polaritons) in a vdW crystal (α-phase molybdenum trioxide) can be directed along forbidden directions by inducing an optical topological transition, which emerges when the slab is placed on a substrate with a given negative permittivity (4H–silicon carbide). By visualizing the transition in real space, we observe exotic polaritonic states between mutually orthogonal hyperbolic regimes, which unveil the topological origin of the transition: a gap opening in the dispersion. This work provides insights into optical topological transitions in vdW crystals, which introduce a route to direct light at the nanoscale.","lang":"eng"}],"file_date_updated":"2021-04-19T11:17:29Z","date_updated":"2026-04-02T13:58:21Z","ddc":["530"],"has_accepted_license":"1","date_published":"2021-04-02T00:00:00Z","fulldoi":"https://doi.org/10.1126/sciadv.abf2690","article_type":"original","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png"},"day":"02","isi":1,"citation":{"ieee":"J. Duan <i>et al.</i>, “Enabling propagation of anisotropic polaritons along forbidden directions via a topological transition,” <i>Science Advances</i>, vol. 7, no. 14. AAAS, 2021.","apa":"Duan, J., Álvarez-Pérez, G., Voronin, K. V., Prieto Gonzalez, I., Taboada-Gutiérrez, J., Volkov, V. S., … Alonso-González, P. (2021). Enabling propagation of anisotropic polaritons along forbidden directions via a topological transition. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.abf2690\">https://doi.org/10.1126/sciadv.abf2690</a>","ista":"Duan J, Álvarez-Pérez G, Voronin KV, Prieto Gonzalez I, Taboada-Gutiérrez J, Volkov VS, Martín-Sánchez J, Nikitin AY, Alonso-González P. 2021. Enabling propagation of anisotropic polaritons along forbidden directions via a topological transition. Science Advances. 7(14), eabf2690.","ama":"Duan J, Álvarez-Pérez G, Voronin KV, et al. Enabling propagation of anisotropic polaritons along forbidden directions via a topological transition. <i>Science Advances</i>. 2021;7(14). doi:<a href=\"https://doi.org/10.1126/sciadv.abf2690\">10.1126/sciadv.abf2690</a>","mla":"Duan, J., et al. “Enabling Propagation of Anisotropic Polaritons along Forbidden Directions via a Topological Transition.” <i>Science Advances</i>, vol. 7, no. 14, eabf2690, AAAS, 2021, doi:<a href=\"https://doi.org/10.1126/sciadv.abf2690\">10.1126/sciadv.abf2690</a>.","short":"J. Duan, G. Álvarez-Pérez, K.V. Voronin, I. Prieto Gonzalez, J. Taboada-Gutiérrez, V.S. Volkov, J. Martín-Sánchez, A.Y. Nikitin, P. Alonso-González, Science Advances 7 (2021).","chicago":"Duan, J., G. Álvarez-Pérez, K. V. Voronin, Ivan Prieto Gonzalez, J. Taboada-Gutiérrez, V. S. Volkov, J. Martín-Sánchez, A. Y. Nikitin, and P. Alonso-González. “Enabling Propagation of Anisotropic Polaritons along Forbidden Directions via a Topological Transition.” <i>Science Advances</i>. AAAS, 2021. <a href=\"https://doi.org/10.1126/sciadv.abf2690\">https://doi.org/10.1126/sciadv.abf2690</a>."},"year":"2021","acknowledgement":"G.Á.-P. and J.T.-G. acknowledge support through the Severo Ochoa Program from the government of the Principality of Asturias (grant nos. PA20-PF-BP19-053 and PA-18-PF-BP17-126, respectively). K.V.V. and V.S.V. acknowledge the Ministry of Science and Higher Education of the Russian Federation (no. 0714-2020-0002). J. M.-S. acknowledges financial support through the Ramón y Cajal Program from the government of Spain and FSE (RYC2018-026196-I). A.Y.N. acknowledges the Spanish Ministry of Science, Innovation and Universities (national project no. MAT201788358-C3-3-R), and the Basque Department of Education (PIBA-2020-1-0014). P.A.-G. acknowledges support from the European Research Council under starting grant no. 715496, 2DNANOPTICA. ","publisher":"AAAS","_id":"9334","status":"public","oa_version":"Published Version","external_id":{"isi":["000636455600027"],"pmid":["33811076"]},"issue":"14","file":[{"relation":"main_file","checksum":"4b383d4a1d484a71bbc64ecf401bbdbb","access_level":"open_access","creator":"dernst","date_updated":"2021-04-19T11:17:29Z","content_type":"application/pdf","file_id":"9343","success":1,"date_created":"2021-04-19T11:17:29Z","file_size":717489,"file_name":"2021_ScienceAdv_Duan.pdf"}],"intvolume":"         7"}]