[{"acknowledgement":"We would like to acknowledge Bioimaging and Life Science Facilities at IST Austria for continuous support and also the Plant Sciences Core Facility of CEITEC Masaryk University for their support with obtaining a part of the scientific data. We gratefully acknowledge Lindy Abas for help with ABP1::GFP-ABP1 construct design. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program [grant agreement no. 742985] and Austrian Science Fund (FWF) [I 3630-B25] to J.F.; DOC Fellowship of the Austrian Academy of Sciences to L.L.; the European Structural and Investment Funds, Operational Programme Research, Development and Education - Project „MSCAfellow@MUNI“ [CZ.02.2.69/0.0/0.0/17_050/0008496] to M.P.. This project was also supported by the Czech Science Foundation [GA 20-20860Y] to M.Z and MEYS CR [project no.CZ.02.1.01/0.0/0.0/16_019/0000738] to M. Č.","has_accepted_license":"1","publication_status":"published","oa":1,"file_date_updated":"2021-02-04T07:49:25Z","volume":303,"date_updated":"2026-08-21T22:30:46Z","publication":"Plant Science","month":"02","quality_controlled":"1","article_type":"original","type":"journal_article","abstract":[{"text":"Auxin is a major plant growth regulator, but current models on auxin perception and signaling cannot explain the whole plethora of auxin effects, in particular those associated with rapid responses. A possible candidate for a component of additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1), whose function in planta remains unclear.\r\nHere we combined expression analysis with gain- and loss-of-function approaches to analyze the role of ABP1 in plant development. ABP1 shows a broad expression largely overlapping with, but not regulated by, transcriptional auxin response activity. Furthermore, ABP1 activity is not essential for the transcriptional auxin signaling. Genetic in planta analysis revealed that abp1 loss-of-function mutants show largely normal development with minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show a broad range of growth and developmental defects, including root and hypocotyl growth and bending, lateral root and leaf development, bolting, as well as response to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular aggregation.\r\nThe gain-of-function analysis suggests a broad, but still mechanistically unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function mutants by a functional redundancy.","lang":"eng"}],"ddc":["580"],"department":[{"_id":"JiFr"},{"_id":"Bio"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ieee":"Z. Gelová <i>et al.</i>, “Developmental roles of auxin binding protein 1 in Arabidopsis thaliana,” <i>Plant Science</i>, vol. 303. Elsevier, 2021.","apa":"Gelová, Z., Gallei, M. C., Pernisová, M., Brunoud, G., Zhang, X., Glanc, M., … Friml, J. (2021). Developmental roles of auxin binding protein 1 in Arabidopsis thaliana. <i>Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">https://doi.org/10.1016/j.plantsci.2020.110750</a>","ista":"Gelová Z, Gallei MC, Pernisová M, Brunoud G, Zhang X, Glanc M, Li L, Michalko J, Pavlovicova Z, Verstraeten I, Han H, Hajny J, Hauschild R, Čovanová M, Zwiewka M, Hörmayer L, Fendrych M, Xu T, Vernoux T, Friml J. 2021. Developmental roles of auxin binding protein 1 in Arabidopsis thaliana. Plant Science. 303, 110750.","ama":"Gelová Z, Gallei MC, Pernisová M, et al. Developmental roles of auxin binding protein 1 in Arabidopsis thaliana. <i>Plant Science</i>. 2021;303. doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">10.1016/j.plantsci.2020.110750</a>","mla":"Gelová, Zuzana, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis Thaliana.” <i>Plant Science</i>, vol. 303, 110750, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">10.1016/j.plantsci.2020.110750</a>.","short":"Z. Gelová, M.C. Gallei, M. Pernisová, G. Brunoud, X. Zhang, M. Glanc, L. Li, J. Michalko, Z. Pavlovicova, I. Verstraeten, H. Han, J. Hajny, R. Hauschild, M. Čovanová, M. Zwiewka, L. Hörmayer, M. Fendrych, T. Xu, T. Vernoux, J. Friml, Plant Science 303 (2021).","chicago":"Gelová, Zuzana, Michelle C Gallei, Markéta Pernisová, Géraldine Brunoud, Xixi Zhang, Matous Glanc, Lanxin Li, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis Thaliana.” <i>Plant Science</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">https://doi.org/10.1016/j.plantsci.2020.110750</a>."},"publisher":"Elsevier","author":[{"first_name":"Zuzana","full_name":"Gelová, Zuzana","orcid":"0000-0003-4783-1752","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","last_name":"Gelová"},{"full_name":"Gallei, Michelle C","first_name":"Michelle C","orcid":"0000-0003-1286-7368","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Markéta","full_name":"Pernisová, Markéta","last_name":"Pernisová"},{"full_name":"Brunoud, Géraldine","first_name":"Géraldine","last_name":"Brunoud"},{"id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","last_name":"Zhang","orcid":"0000-0001-7048-4627","first_name":"Xixi","full_name":"Zhang, Xixi"},{"full_name":"Glanc, Matous","first_name":"Matous","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","last_name":"Glanc","orcid":"0000-0003-0619-7783"},{"first_name":"Lanxin","full_name":"Li, Lanxin","orcid":"0000-0002-5607-272X","last_name":"Li","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Michalko, Jaroslav","first_name":"Jaroslav","id":"483727CA-F248-11E8-B48F-1D18A9856A87","last_name":"Michalko"},{"last_name":"Pavlovicova","first_name":"Zlata","full_name":"Pavlovicova, Zlata"},{"last_name":"Verstraeten","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge","first_name":"Inge"},{"full_name":"Han, Huibin","first_name":"Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87","last_name":"Han"},{"full_name":"Hajny, Jakub","first_name":"Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","last_name":"Hajny","orcid":"0000-0003-2140-7195"},{"first_name":"Robert","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"last_name":"Čovanová","full_name":"Čovanová, Milada","first_name":"Milada"},{"full_name":"Zwiewka, Marta","first_name":"Marta","last_name":"Zwiewka"},{"last_name":"Hörmayer","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8295-2926","full_name":"Hörmayer, Lukas","first_name":"Lukas"},{"full_name":"Fendrych, Matyas","first_name":"Matyas","last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9767-8699"},{"last_name":"Xu","full_name":"Xu, Tongda","first_name":"Tongda"},{"full_name":"Vernoux, Teva","first_name":"Teva","last_name":"Vernoux"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří"}],"doi":"10.1016/j.plantsci.2020.110750","pmid":1,"oa_version":"Published Version","status":"public","external_id":{"pmid":["33487339"],"isi":["000614154500001"]},"title":"Developmental roles of auxin binding protein 1 in Arabidopsis thaliana","date_published":"2021-02-01T00:00:00Z","article_number":"110750","publication_identifier":{"issn":["0168-9452"]},"scopus_import":"1","license":"https://creativecommons.org/licenses/by/4.0/","ec_funded":1,"article_processing_charge":"Yes (via OA deal)","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF"},{"name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root","grant_number":"25351","_id":"26B4D67E-B435-11E9-9278-68D0E5697425"}],"isi":1,"date_created":"2020-12-09T14:48:28Z","year":"2021","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"corr_author":"1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"11626"},{"id":"10083","relation":"dissertation_contains","status":"public"}]},"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"file_id":"9083","success":1,"creator":"dernst","date_updated":"2021-02-04T07:49:25Z","file_name":"2021_PlantScience_Gelova.pdf","access_level":"open_access","content_type":"application/pdf","checksum":"a7f2562bdca62d67dfa88e271b62a629","file_size":12563728,"date_created":"2021-02-04T07:49:25Z","relation":"main_file"}],"_id":"8931","intvolume":"       303","language":[{"iso":"eng"}],"keyword":["Agronomy and Crop Science","Plant Science","Genetics","General Medicine"],"day":"01"},{"month":"09","publication":"Research Square","date_updated":"2026-08-21T22:30:46Z","oa":1,"publication_status":"draft","acknowledgement":"We thank Nataliia Gnyliukh and Lukas Hörmayer for technical assistance and Nadine Paris for sharing PM-Cyto seeds. We gratefully acknowledge Life Science, Machine Shop and Bioimaging Facilities of IST Austria. This project has received funding from the European Research Council Advanced Grant (ETAP-742985) and the Austrian Science Fund (FWF) I 3630-B25 to J.F., the National Institutes of Health (GM067203) to W.M.G., the Netherlands Organization for Scientific Research (NWO; VIDI-864.13.001.), the Research Foundation-Flanders (FWO; Odysseus II G0D0515N) and a European Research Council Starting Grant (TORPEDO-714055) to W.S. and B.D.R., the VICI grant (865.14.001) from the Netherlands Organization for Scientific Research to M.R and D.W., the Australian Research Council and China National Distinguished Expert Project (WQ20174400441) to S.S., the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and 20H05910),  the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 and the DOC Fellowship of the Austrian Academy of Sciences to L.L., the China Scholarship Council to J.C.","title":"Cell surface and intracellular auxin signalling for H+-fluxes in root growth","date_published":"2021-09-09T00:00:00Z","article_number":"266395","status":"public","doi":"10.21203/rs.3.rs-266395/v3","oa_version":"Preprint","citation":{"ieee":"L. Li <i>et al.</i>, “Cell surface and intracellular auxin signalling for H+-fluxes in root growth,” <i>Research Square</i>. .","ista":"Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez Solovey L, Merrin J, Chen J, Shabala L, Smet W, Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D, Kinoshita T, Gray WM, Friml J. Cell surface and intracellular auxin signalling for H+-fluxes in root growth. Research Square, 266395.","apa":"Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez Solovey, L., Merrin, J., … Friml, J. (n.d.). Cell surface and intracellular auxin signalling for H+-fluxes in root growth. <i>Research Square</i>. <a href=\"https://doi.org/10.21203/rs.3.rs-266395/v3\">https://doi.org/10.21203/rs.3.rs-266395/v3</a>","ama":"Li L, Verstraeten I, Roosjen M, et al. Cell surface and intracellular auxin signalling for H+-fluxes in root growth. <i>Research Square</i>. doi:<a href=\"https://doi.org/10.21203/rs.3.rs-266395/v3\">10.21203/rs.3.rs-266395/v3</a>","mla":"Li, Lanxin, et al. “Cell Surface and Intracellular Auxin Signalling for H+-Fluxes in Root Growth.” <i>Research Square</i>, 266395, doi:<a href=\"https://doi.org/10.21203/rs.3.rs-266395/v3\">10.21203/rs.3.rs-266395/v3</a>.","short":"L. Li, I. Verstraeten, M. Roosjen, K. Takahashi, L. Rodriguez Solovey, J. Merrin, J. Chen, L. Shabala, W. Smet, H. Ren, S. Vanneste, S. Shabala, B. De Rybel, D. Weijers, T. Kinoshita, W.M. Gray, J. Friml, Research Square (n.d.).","chicago":"Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez Solovey, Jack Merrin, Jian Chen, et al. “Cell Surface and Intracellular Auxin Signalling for H+-Fluxes in Root Growth.” <i>Research Square</i>, n.d. <a href=\"https://doi.org/10.21203/rs.3.rs-266395/v3\">https://doi.org/10.21203/rs.3.rs-266395/v3</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Li, Lanxin","first_name":"Lanxin","orcid":"0000-0002-5607-272X","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","last_name":"Li"},{"orcid":"0000-0001-7241-2328","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten","first_name":"Inge","full_name":"Verstraeten, Inge"},{"full_name":"Roosjen, Mark","first_name":"Mark","last_name":"Roosjen"},{"full_name":"Takahashi, Koji","first_name":"Koji","last_name":"Takahashi"},{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","last_name":"Rodriguez Solovey","orcid":"0000-0002-7244-7237","first_name":"Lesia","full_name":"Rodriguez Solovey, Lesia"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","first_name":"Jack"},{"last_name":"Chen","full_name":"Chen, Jian","first_name":"Jian"},{"last_name":"Shabala","first_name":"Lana","full_name":"Shabala, Lana"},{"last_name":"Smet","first_name":"Wouter","full_name":"Smet, Wouter"},{"first_name":"Hong","full_name":"Ren, Hong","last_name":"Ren"},{"full_name":"Vanneste, Steffen","first_name":"Steffen","last_name":"Vanneste"},{"last_name":"Shabala","full_name":"Shabala, Sergey","first_name":"Sergey"},{"full_name":"De Rybel, Bert","first_name":"Bert","last_name":"De Rybel"},{"last_name":"Weijers","full_name":"Weijers, Dolf","first_name":"Dolf"},{"first_name":"Toshinori","full_name":"Kinoshita, Toshinori","last_name":"Kinoshita"},{"first_name":"William M.","full_name":"Gray, William M.","last_name":"Gray"},{"full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"type":"preprint","abstract":[{"text":"Growth regulation tailors plant development to its environment. A showcase is response to gravity, where shoots bend up and roots down1. This paradox is based on opposite effects of the phytohormone auxin, which promotes cell expansion in shoots, while inhibiting it in roots via a yet unknown cellular mechanism2. Here, by combining microfluidics, live imaging, genetic engineering and phospho-proteomics in Arabidopsis thaliana, we advance our understanding how auxin inhibits root growth. We show that auxin activates two distinct, antagonistically acting signalling pathways that converge on the rapid regulation of the apoplastic pH, a causative growth determinant. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts with and mediates phosphorylation and activation of plasma membrane H+-ATPases for apoplast acidification, while intracellular canonical auxin signalling promotes net cellular H+-influx, causing apoplast alkalinisation. The simultaneous activation of these two counteracting mechanisms poises the root for a rapid, fine-tuned growth modulation while navigating complex soil environment.","lang":"eng"}],"department":[{"_id":"JiFr"},{"_id":"NanoFab"}],"project":[{"grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"International IST Doctoral Program"},{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"},{"name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root","grant_number":"25351","_id":"26B4D67E-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"No","ec_funded":1,"publication_identifier":{"issn":["2693-5015"]},"_id":"10095","language":[{"iso":"eng"}],"day":"09","corr_author":"1","related_material":{"record":[{"status":"public","relation":"later_version","id":"10223"},{"relation":"dissertation_contains","status":"public","id":"10083"}]},"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"Bio"}],"date_created":"2021-10-06T08:56:22Z","year":"2021","main_file_link":[{"open_access":"1","url":"https://www.doi.org/10.21203/rs.3.rs-266395/v3"}]},{"month":"12","quality_controlled":"1","publication":"Proceedings of the National Academy of Sciences of the United States of America","date_updated":"2026-08-21T22:30:53Z","volume":118,"oa":1,"file_date_updated":"2021-12-15T08:59:40Z","publication_status":"published","acknowledgement":"We gratefully thank Julie Neveu and Dr. Amanda Barranco of the Grégory Vert laboratory for help preparing plants in France, Dr. Zuzana Gelova for help and advice with protoplast generation, Dr. Stéphane Vassilopoulos and Dr. Florian Schur for advice regarding EM tomography, Alejandro Marquiegui Alvaro for help with material generation, and Dr. Lukasz Kowalski for generously gifting us the mWasabi protein. This research was supported by the Scientific Service Units of Institute of Science and Technology Austria (IST Austria) through resources provided by the Electron Microscopy Facility, Lab Support Facility (particularly Dorota Jaworska), and the Bioimaging Facility. We acknowledge the Advanced Microscopy Facility of the Vienna BioCenter Core Facilities for use of the 3D SIM. For the mass spectrometry analysis of proteins, we acknowledge the University of Natural Resources and Life Sciences (BOKU) Core Facility Mass Spectrometry. This work was supported by the following funds: A.J. is supported by funding from the Austrian Science Fund I3630B25 to J.F. P.M. and E.B. are supported by Agence Nationale de la Recherche ANR-11-EQPX-0029 Morphoscope2 and ANR-10-INBS-04 France BioImaging. S.Y.B. is supported by the NSF No. 1121998 and 1614915. J.W. and D.V.D. are supported by the European Research Council Grant 682436 (to D.V.D.), a China Scholarship Council Grant 201508440249 (to J.W.), and by a Ghent University Special Research Co-funding Grant ST01511051 (to J.W.).","has_accepted_license":"1","external_id":{"isi":["000736417600043"],"pmid":["34907016"]},"date_published":"2021-12-14T00:00:00Z","article_number":"e2113046118","title":"The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis","status":"public","doi":"10.1073/pnas.2113046118","pmid":1,"oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Johnson, Alexander J, Dana A Dahhan, Nataliia Gnyliukh, Walter Kaufmann, Vanessa Zheden, Tommaso Costanzo, Pierre Mahou, et al. “The TPLATE Complex Mediates Membrane Bending during Plant Clathrin-Mediated Endocytosis.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2021. <a href=\"https://doi.org/10.1073/pnas.2113046118\">https://doi.org/10.1073/pnas.2113046118</a>.","short":"A.J. Johnson, D.A. Dahhan, N. Gnyliukh, W. Kaufmann, V. Zheden, T. Costanzo, P. Mahou, M. Hrtyan, J. Wang, J.L. Aguilera Servin, D. van Damme, E. Beaurepaire, M. Loose, S.Y. Bednarek, J. Friml, Proceedings of the National Academy of Sciences of the United States of America 118 (2021).","mla":"Johnson, Alexander J., et al. “The TPLATE Complex Mediates Membrane Bending during Plant Clathrin-Mediated Endocytosis.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 51, e2113046118, National Academy of Sciences, 2021, doi:<a href=\"https://doi.org/10.1073/pnas.2113046118\">10.1073/pnas.2113046118</a>.","ama":"Johnson AJ, Dahhan DA, Gnyliukh N, et al. The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2021;118(51). doi:<a href=\"https://doi.org/10.1073/pnas.2113046118\">10.1073/pnas.2113046118</a>","ieee":"A. J. Johnson <i>et al.</i>, “The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 118, no. 51. National Academy of Sciences, 2021.","ista":"Johnson AJ, Dahhan DA, Gnyliukh N, Kaufmann W, Zheden V, Costanzo T, Mahou P, Hrtyan M, Wang J, Aguilera Servin JL, van Damme D, Beaurepaire E, Loose M, Bednarek SY, Friml J. 2021. The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis. Proceedings of the National Academy of Sciences of the United States of America. 118(51), e2113046118.","apa":"Johnson, A. J., Dahhan, D. A., Gnyliukh, N., Kaufmann, W., Zheden, V., Costanzo, T., … Friml, J. (2021). The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2113046118\">https://doi.org/10.1073/pnas.2113046118</a>"},"publisher":"National Academy of Sciences","author":[{"first_name":"Alexander J","full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson"},{"last_name":"Dahhan","full_name":"Dahhan, Dana A","first_name":"Dana A"},{"full_name":"Gnyliukh, Nataliia","first_name":"Nataliia","id":"390C1120-F248-11E8-B48F-1D18A9856A87","last_name":"Gnyliukh","orcid":"0000-0002-2198-0509"},{"first_name":"Walter","full_name":"Kaufmann, Walter","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9735-5315"},{"orcid":"0000-0002-9438-4783","last_name":"Zheden","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","full_name":"Zheden, Vanessa"},{"id":"D93824F4-D9BA-11E9-BB12-F207E6697425","last_name":"Costanzo","orcid":"0000-0001-9732-3815","first_name":"Tommaso","full_name":"Costanzo, Tommaso"},{"last_name":"Mahou","first_name":"Pierre","full_name":"Mahou, Pierre"},{"last_name":"Hrtyan","id":"45A71A74-F248-11E8-B48F-1D18A9856A87","first_name":"Mónika","full_name":"Hrtyan, Mónika"},{"last_name":"Wang","first_name":"Jie","full_name":"Wang, Jie"},{"id":"2A67C376-F248-11E8-B48F-1D18A9856A87","last_name":"Aguilera Servin","orcid":"0000-0002-2862-8372","full_name":"Aguilera Servin, Juan L","first_name":"Juan L"},{"first_name":"Daniël","full_name":"van Damme, Daniël","last_name":"van Damme"},{"first_name":"Emmanuel","full_name":"Beaurepaire, Emmanuel","last_name":"Beaurepaire"},{"orcid":"0000-0001-7309-9724","last_name":"Loose","id":"462D4284-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","full_name":"Loose, Martin"},{"first_name":"Sebastian Y","full_name":"Bednarek, Sebastian Y","last_name":"Bednarek"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří"}],"type":"journal_article","abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis is the major route of entry of cargos into cells and thus underpins many physiological processes. During endocytosis, an area of flat membrane is remodeled by proteins to create a spherical vesicle against intracellular forces. The protein machinery which mediates this membrane bending in plants is unknown. However, it is known that plant endocytosis is actin independent, thus indicating that plants utilize a unique mechanism to mediate membrane bending against high-turgor pressure compared to other model systems. Here, we investigate the TPLATE complex, a plant-specific endocytosis protein complex. It has been thought to function as a classical adaptor functioning underneath the clathrin coat. However, by using biochemical and advanced live microscopy approaches, we found that TPLATE is peripherally associated with clathrin-coated vesicles and localizes at the rim of endocytosis events. As this localization is more fitting to the protein machinery involved in membrane bending during endocytosis, we examined cells in which the TPLATE complex was disrupted and found that the clathrin structures present as flat patches. This suggests a requirement of the TPLATE complex for membrane bending during plant clathrin–mediated endocytosis. Next, we used in vitro biophysical assays to confirm that the TPLATE complex possesses protein domains with intrinsic membrane remodeling activity. These results redefine the role of the TPLATE complex and implicate it as a key component of the evolutionarily distinct plant endocytosis mechanism, which mediates endocytic membrane bending against the high-turgor pressure in plant cells."}],"department":[{"_id":"JiFr"},{"_id":"MaLo"},{"_id":"EvBe"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"ddc":["580"],"article_type":"original","project":[{"_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF"}],"article_processing_charge":"No","scopus_import":"1","issue":"51","publication_identifier":{"eissn":["1091-6490"]},"_id":"9887","intvolume":"       118","language":[{"iso":"eng"}],"day":"14","corr_author":"1","related_material":{"link":[{"relation":"earlier_version","url":"https://doi.org/10.1101/2021.04.26.441441"}],"record":[{"id":"14988","relation":"research_data","status":"public"},{"relation":"dissertation_contains","status":"public","id":"14510"}]},"file":[{"access_level":"open_access","content_type":"application/pdf","checksum":"8d01e72e22c4fb1584e72d8601947069","file_size":2757340,"date_created":"2021-12-15T08:59:40Z","relation":"main_file","success":1,"creator":"cchlebak","file_id":"10546","date_updated":"2021-12-15T08:59:40Z","file_name":"2021_PNAS_Johnson.pdf"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"Bio"}],"year":"2021","date_created":"2021-08-11T14:11:43Z","isi":1},{"doi":"10.1007/978-1-0716-1522-5_19","oa_version":"None","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ama":"Kaufmann W, Kleindienst D, Harada H, Shigemoto R. High-Resolution localization and quantitation of membrane proteins by SDS-digested freeze-fracture replica labeling (SDS-FRL). In: <i>Receptor and Ion Channel Detection in the Brain</i>. Vol 169. Neuromethods. New York: Humana Press; 2021:267-283. doi:<a href=\"https://doi.org/10.1007/978-1-0716-1522-5_19\">10.1007/978-1-0716-1522-5_19</a>","ieee":"W. Kaufmann, D. Kleindienst, H. Harada, and R. Shigemoto, “High-Resolution localization and quantitation of membrane proteins by SDS-digested freeze-fracture replica labeling (SDS-FRL),” in <i>Receptor and Ion Channel Detection in the Brain</i>, vol. 169, New York: Humana Press, 2021, pp. 267–283.","ista":"Kaufmann W, Kleindienst D, Harada H, Shigemoto R. 2021.High-Resolution localization and quantitation of membrane proteins by SDS-digested freeze-fracture replica labeling (SDS-FRL). In: Receptor and Ion Channel Detection in the Brain. Neuromethods, vol. 169, 267–283.","apa":"Kaufmann, W., Kleindienst, D., Harada, H., &#38; Shigemoto, R. (2021). High-Resolution localization and quantitation of membrane proteins by SDS-digested freeze-fracture replica labeling (SDS-FRL). In <i>Receptor and Ion Channel Detection in the Brain</i> (Vol. 169, pp. 267–283). New York: Humana Press. <a href=\"https://doi.org/10.1007/978-1-0716-1522-5_19\">https://doi.org/10.1007/978-1-0716-1522-5_19</a>","chicago":"Kaufmann, Walter, David Kleindienst, Harumi Harada, and Ryuichi Shigemoto. “High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL).” In <i>Receptor and Ion Channel Detection in the Brain</i>, 169:267–83. Neuromethods. New York: Humana Press, 2021. <a href=\"https://doi.org/10.1007/978-1-0716-1522-5_19\">https://doi.org/10.1007/978-1-0716-1522-5_19</a>.","mla":"Kaufmann, Walter, et al. “High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL).” <i>Receptor and Ion Channel Detection in the Brain</i>, vol. 169, Humana Press, 2021, pp. 267–83, doi:<a href=\"https://doi.org/10.1007/978-1-0716-1522-5_19\">10.1007/978-1-0716-1522-5_19</a>.","short":"W. Kaufmann, D. Kleindienst, H. Harada, R. Shigemoto, in:, Receptor and Ion Channel Detection in the Brain, Humana Press, New York, 2021, pp. 267–283."},"publisher":"Humana Press","place":"New York","author":[{"first_name":"Walter","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kleindienst, David","first_name":"David","last_name":"Kleindienst","id":"42E121A4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Harumi","full_name":"Harada, Harumi","id":"2E55CDF2-F248-11E8-B48F-1D18A9856A87","last_name":"Harada","orcid":"0000-0001-7429-7896"},{"last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","first_name":"Ryuichi","full_name":"Shigemoto, Ryuichi"}],"date_published":"2021-07-27T00:00:00Z","title":"High-Resolution localization and quantitation of membrane proteins by SDS-digested freeze-fracture replica labeling (SDS-FRL)","status":"public","page":"267-283","abstract":[{"text":"High-resolution visualization and quantification of membrane proteins contribute to the understanding of their functions and the roles they play in physiological and pathological conditions. Sodium dodecyl sulfate-digested freeze-fracture replica labeling (SDS-FRL) is a powerful electron microscopy method to study quantitatively the two-dimensional distribution of transmembrane proteins and their tightly associated proteins. During treatment with SDS, intracellular organelles and proteins not anchored to the replica are dissolved, whereas integral membrane proteins captured and stabilized by carbon/platinum deposition remain on the replica. Their intra- and extracellular domains become exposed on the surface of the replica, facilitating the accessibility of antibodies and, therefore, providing higher labeling efficiency than those obtained with other immunoelectron microscopy techniques. In this chapter, we describe the protocols of SDS-FRL adapted for mammalian brain samples, and optimization of the SDS treatment to increase the labeling efficiency for quantification of Cav2.1, the alpha subunit of P/Q-type voltage-dependent calcium channels utilizing deep learning algorithms.","lang":"eng"}],"type":"book_chapter","ddc":["573"],"series_title":"Neuromethods","department":[{"_id":"RySh"},{"_id":"EM-Fac"}],"date_updated":"2026-08-21T22:31:04Z","volume":169,"month":"07","quality_controlled":"1","publication":"Receptor and Ion Channel Detection in the Brain","acknowledgement":"This work was supported by the European Union (European Research Council Advanced grant no. 694539 and Human Brain Project Ref. 720270 to R. S.) and the Austrian Academy of Sciences (DOC fellowship to D.K.).","has_accepted_license":"1","publication_status":"published","_id":"9756","intvolume":"       169","language":[{"iso":"eng"}],"day":"27","keyword":["Freeze-fracture replica: Deep learning","Immunogold labeling","Integral membrane protein","Electron microscopy"],"corr_author":"1","related_material":{"record":[{"id":"9562","status":"public","relation":"dissertation_contains"}]},"date_created":"2021-07-30T09:34:56Z","year":"2021","article_processing_charge":"No","project":[{"_id":"25CA28EA-B435-11E9-9278-68D0E5697425","grant_number":"694539","call_identifier":"H2020","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour"},{"grant_number":"720270","_id":"25CBA828-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Human Brain Project Specific Grant Agreement 1"}],"alternative_title":["Neuromethods"],"scopus_import":"1","publication_identifier":{"isbn":["9781071615218"],"eisbn":["9781071615225"]},"das_tickbox":"1","ec_funded":1},{"license":"https://opensource.org/licenses/GPL-3.0","has_accepted_license":"1","oa":1,"file_date_updated":"2021-10-08T08:46:04Z","date_updated":"2026-08-21T22:31:04Z","month":"12","abstract":[{"text":"Pattern separation is a fundamental brain computation that converts small differences in input patterns into large differences in output patterns. Several synaptic mechanisms of pattern separation have been proposed, including code expansion, inhibition and plasticity; however, which of these mechanisms play a role in the entorhinal cortex (EC)–dentate gyrus (DG)–CA3 circuit, a classical pattern separation circuit, remains unclear. Here we show that a biologically realistic, full-scale EC–DG–CA3 circuit model, including granule cells (GCs) and parvalbumin-positive inhibitory interneurons (PV+-INs) in the DG, is an efficient pattern separator. Both external gamma-modulated inhibition and internal lateral inhibition mediated by PV+-INs substantially contributed to pattern separation. Both local connectivity and fast signaling at GC–PV+-IN synapses were important for maximum effectiveness. Similarly, mossy fiber synapses with conditional detonator properties contributed to pattern separation. By contrast, perforant path synapses with Hebbian synaptic plasticity and direct EC–CA3 connection shifted the network towards pattern completion. Our results demonstrate that the specific properties of cells and synapses optimize higher-order computations in biological networks and might be useful to improve the deep learning capabilities of technical networks.","lang":"eng"}],"type":"software","date_created":"2021-10-08T06:44:22Z","department":[{"_id":"PeJo"},{"_id":"ScienComp"}],"year":"2021","ddc":["005"],"related_material":{"record":[{"id":"10816","status":"public","relation":"used_for_analysis_in"}],"link":[{"url":"https://ist.ac.at/en/news/spot-the-difference/","description":"News on IST Webpage","relation":"press_release"}]},"citation":{"ama":"Guzmán J, Schlögl A, Espinoza Martinez C, Zhang X, Suter B, Jonas PM. How connectivity rules and synaptic properties shape the efficacy of pattern separation in the entorhinal cortex–dentate gyrus–CA3 network. 2021. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:10110\">10.15479/AT:ISTA:10110</a>","apa":"Guzmán, J., Schlögl, A., Espinoza Martinez, C., Zhang, X., Suter, B., &#38; Jonas, P. M. (2021). How connectivity rules and synaptic properties shape the efficacy of pattern separation in the entorhinal cortex–dentate gyrus–CA3 network. IST Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:10110\">https://doi.org/10.15479/AT:ISTA:10110</a>","ista":"Guzmán J, Schlögl A, Espinoza Martinez C, Zhang X, Suter B, Jonas PM. 2021. How connectivity rules and synaptic properties shape the efficacy of pattern separation in the entorhinal cortex–dentate gyrus–CA3 network, IST Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:10110\">10.15479/AT:ISTA:10110</a>.","ieee":"J. Guzmán, A. Schlögl, C. Espinoza Martinez, X. Zhang, B. Suter, and P. M. Jonas, “How connectivity rules and synaptic properties shape the efficacy of pattern separation in the entorhinal cortex–dentate gyrus–CA3 network.” IST Austria, 2021.","chicago":"Guzmán, José, Alois Schlögl, Claudia  Espinoza Martinez, Xiaomin Zhang, Benjamin Suter, and Peter M Jonas. “How Connectivity Rules and Synaptic Properties Shape the Efficacy of Pattern Separation in the Entorhinal Cortex–Dentate Gyrus–CA3 Network.” IST Austria, 2021. <a href=\"https://doi.org/10.15479/AT:ISTA:10110\">https://doi.org/10.15479/AT:ISTA:10110</a>.","short":"J. Guzmán, A. Schlögl, C. Espinoza Martinez, X. Zhang, B. Suter, P.M. Jonas, (2021).","mla":"Guzmán, José, et al. <i>How Connectivity Rules and Synaptic Properties Shape the Efficacy of Pattern Separation in the Entorhinal Cortex–Dentate Gyrus–CA3 Network</i>. IST Austria, 2021, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:10110\">10.15479/AT:ISTA:10110</a>."},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","publisher":"IST Austria","tmp":{"short":"GPL 3.0","legal_code_url":"https://www.gnu.org/licenses/gpl-3.0.en.html","name":"GNU General Public License 3.0"},"author":[{"orcid":"0000-0003-2209-5242","last_name":"Guzmán","id":"30CC5506-F248-11E8-B48F-1D18A9856A87","first_name":"José","full_name":"Guzmán, José"},{"full_name":"Schlögl, Alois","first_name":"Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl","orcid":"0000-0002-5621-8100"},{"first_name":"Claudia ","full_name":"Espinoza Martinez, Claudia ","orcid":"0000-0003-4710-2082","id":"31FFEE2E-F248-11E8-B48F-1D18A9856A87","last_name":"Espinoza Martinez"},{"first_name":"Xiaomin","full_name":"Zhang, Xiaomin","orcid":"0000-0003-0256-6529","last_name":"Zhang","id":"423EC9C2-F248-11E8-B48F-1D18A9856A87"},{"id":"4952F31E-F248-11E8-B48F-1D18A9856A87","last_name":"Suter","orcid":"0000-0002-9885-6936","first_name":"Benjamin","full_name":"Suter, Benjamin"},{"first_name":"Peter M","full_name":"Jonas, Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","last_name":"Jonas","orcid":"0000-0001-5001-4804"}],"file":[{"relation":"main_file","file_size":332990101,"date_created":"2021-10-08T08:46:04Z","content_type":"application/x-zip-compressed","checksum":"f92f8931cad0aa7e411c1715337bf408","access_level":"open_access","file_name":"patternseparation-main (1).zip","date_updated":"2021-10-08T08:46:04Z","file_id":"10114","success":1,"creator":"cchlebak"}],"doi":"10.15479/AT:ISTA:10110","_id":"10110","day":"16","status":"public","date_published":"2021-12-16T00:00:00Z","title":"How connectivity rules and synaptic properties shape the efficacy of pattern separation in the entorhinal cortex–dentate gyrus–CA3 network"},{"_id":"10866","language":[{"iso":"eng"}],"intvolume":"        20","day":"01","keyword":["Mechanical Engineering","Condensed Matter Physics","General Materials Science","General Chemistry","Bioengineering"],"year":"2020","date_created":"2022-03-18T11:37:38Z","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/2004.14599"}],"isi":1,"article_processing_charge":"No","publication_identifier":{"eissn":["1530-6992"],"issn":["1530-6984"]},"scopus_import":"1","issue":"7","status":"public","external_id":{"arxiv":["2004.14599"],"isi":["000548893200082"],"pmid":["32530634"]},"date_published":"2020-07-01T00:00:00Z","title":"Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs","publisher":"American Chemical Society","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"short":"J. Duan, N. Capote-Robayna, J. Taboada-Gutiérrez, G. Álvarez-Pérez, I. Prieto Gonzalez, J. Martín-Sánchez, A.Y. Nikitin, P. Alonso-González, Nano Letters 20 (2020) 5323–5329.","mla":"Duan, Jiahua, et al. “Twisted Nano-Optics: Manipulating Light at the Nanoscale with Twisted Phonon Polaritonic Slabs.” <i>Nano Letters</i>, vol. 20, no. 7, American Chemical Society, 2020, pp. 5323–29, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.0c01673\">10.1021/acs.nanolett.0c01673</a>.","chicago":"Duan, Jiahua, Nathaniel Capote-Robayna, Javier Taboada-Gutiérrez, Gonzalo Álvarez-Pérez, Ivan Prieto Gonzalez, Javier Martín-Sánchez, Alexey Y. Nikitin, and Pablo Alonso-González. “Twisted Nano-Optics: Manipulating Light at the Nanoscale with Twisted Phonon Polaritonic Slabs.” <i>Nano Letters</i>. American Chemical Society, 2020. <a href=\"https://doi.org/10.1021/acs.nanolett.0c01673\">https://doi.org/10.1021/acs.nanolett.0c01673</a>.","ieee":"J. Duan <i>et al.</i>, “Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs,” <i>Nano Letters</i>, vol. 20, no. 7. American Chemical Society, pp. 5323–5329, 2020.","apa":"Duan, J., Capote-Robayna, N., Taboada-Gutiérrez, J., Álvarez-Pérez, G., Prieto Gonzalez, I., Martín-Sánchez, J., … Alonso-González, P. (2020). Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs. <i>Nano Letters</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.nanolett.0c01673\">https://doi.org/10.1021/acs.nanolett.0c01673</a>","ista":"Duan J, Capote-Robayna N, Taboada-Gutiérrez J, Álvarez-Pérez G, Prieto Gonzalez I, Martín-Sánchez J, Nikitin AY, Alonso-González P. 2020. Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs. Nano Letters. 20(7), 5323–5329.","ama":"Duan J, Capote-Robayna N, Taboada-Gutiérrez J, et al. Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs. <i>Nano Letters</i>. 2020;20(7):5323-5329. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.0c01673\">10.1021/acs.nanolett.0c01673</a>"},"author":[{"last_name":"Duan","first_name":"Jiahua","full_name":"Duan, Jiahua"},{"full_name":"Capote-Robayna, Nathaniel","first_name":"Nathaniel","last_name":"Capote-Robayna"},{"full_name":"Taboada-Gutiérrez, Javier","first_name":"Javier","last_name":"Taboada-Gutiérrez"},{"last_name":"Álvarez-Pérez","full_name":"Álvarez-Pérez, Gonzalo","first_name":"Gonzalo"},{"first_name":"Ivan","full_name":"Prieto Gonzalez, Ivan","orcid":"0000-0002-7370-5357","last_name":"Prieto Gonzalez","id":"2A307FE2-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Martín-Sánchez","first_name":"Javier","full_name":"Martín-Sánchez, Javier"},{"last_name":"Nikitin","full_name":"Nikitin, Alexey Y.","first_name":"Alexey Y."},{"full_name":"Alonso-González, Pablo","first_name":"Pablo","last_name":"Alonso-González"}],"doi":"10.1021/acs.nanolett.0c01673","pmid":1,"oa_version":"Preprint","type":"journal_article","abstract":[{"lang":"eng","text":"Recent discoveries have shown that, when two layers of van der Waals (vdW) materials are superimposed with a relative twist angle between them, the electronic properties of the coupled system can be dramatically altered. Here, we demonstrate that a similar concept can be extended to the optics realm, particularly to propagating phonon polaritons–hybrid light-matter interactions. To do this, we fabricate stacks composed of two twisted slabs of a vdW crystal (α-MoO3) supporting anisotropic phonon polaritons (PhPs), and image the propagation of the latter when launched by localized sources. Our images reveal that, under a critical angle, the PhPs isofrequency curve undergoes a topological transition, in which the propagation of PhPs is strongly guided (canalization regime) along predetermined directions without geometric spreading. These results demonstrate a new degree of freedom (twist angle) for controlling the propagation of polaritons at the nanoscale with potential for nanoimaging, (bio)-sensing, or heat management."}],"department":[{"_id":"NanoFab"}],"page":"5323-5329","article_type":"original","publication":"Nano Letters","arxiv":1,"month":"07","quality_controlled":"1","volume":20,"date_updated":"2023-09-05T12:05:58Z","publication_status":"published","oa":1,"acknowledgement":"J.T.-G. and G.Á.-P. acknowledge support through the Severo Ochoa Program from the\r\nGovernment of the Principality of Asturias (nos. PA-18-PF-BP17-126 and PA20-PF-BP19-053,\r\nrespectively). J. M-S acknowledges financial support through the Ramón y Cajal Program from\r\nthe Government of Spain (RYC2018-026196-I). A.Y.N. acknowledges the Spanish Ministry of\r\nScience, Innovation and Universities (national project no. MAT201788358-C3-3-R). P.A.-G.\r\nacknowledges support from the European Research Council under starting grant no. 715496,\r\n2DNANOPTICA."},{"publication":"Microscopy and Microanalysis","quality_controlled":"1","month":"08","volume":26,"date_updated":"2024-10-09T21:08:43Z","article_processing_charge":"No","publication_status":"published","publication_identifier":{"issn":["1431-9276"],"eissn":["1435-8115"]},"issue":"S2","status":"public","title":"Cryo-electron tomography workflows for quantitative analysis of actin networks involved in cell migration","date_published":"2020-08-01T00:00:00Z","corr_author":"1","publisher":"Oxford University Press","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"short":"F. Fäßler, G.A. Dimchev, V.-V. Hodirnau, B. Zens, C. Möhl, F. Bradke, F.K. Schur, Microscopy and Microanalysis 26 (2020) 2518–2519.","mla":"Fäßler, Florian, et al. “Cryo-Electron Tomography Workflows for Quantitative Analysis of Actin Networks Involved in Cell Migration.” <i>Microscopy and Microanalysis</i>, vol. 26, no. S2, Oxford University Press, 2020, pp. 2518–19, doi:<a href=\"https://doi.org/10.1017/s1431927620021881\">10.1017/s1431927620021881</a>.","chicago":"Fäßler, Florian, Georgi A Dimchev, Victor-Valentin Hodirnau, Bettina Zens, Christoph Möhl, Frank Bradke, and Florian KM Schur. “Cryo-Electron Tomography Workflows for Quantitative Analysis of Actin Networks Involved in Cell Migration.” <i>Microscopy and Microanalysis</i>. Oxford University Press, 2020. <a href=\"https://doi.org/10.1017/s1431927620021881\">https://doi.org/10.1017/s1431927620021881</a>.","ista":"Fäßler F, Dimchev GA, Hodirnau V-V, Zens B, Möhl C, Bradke F, Schur FK. 2020. Cryo-electron tomography workflows for quantitative analysis of actin networks involved in cell migration. Microscopy and Microanalysis. 26(S2), 2518–2519.","apa":"Fäßler, F., Dimchev, G. A., Hodirnau, V.-V., Zens, B., Möhl, C., Bradke, F., &#38; Schur, F. K. (2020). Cryo-electron tomography workflows for quantitative analysis of actin networks involved in cell migration. <i>Microscopy and Microanalysis</i>. Oxford University Press. <a href=\"https://doi.org/10.1017/s1431927620021881\">https://doi.org/10.1017/s1431927620021881</a>","ieee":"F. Fäßler <i>et al.</i>, “Cryo-electron tomography workflows for quantitative analysis of actin networks involved in cell migration,” <i>Microscopy and Microanalysis</i>, vol. 26, no. S2. Oxford University Press, pp. 2518–2519, 2020.","ama":"Fäßler F, Dimchev GA, Hodirnau V-V, et al. Cryo-electron tomography workflows for quantitative analysis of actin networks involved in cell migration. <i>Microscopy and Microanalysis</i>. 2020;26(S2):2518-2519. doi:<a href=\"https://doi.org/10.1017/s1431927620021881\">10.1017/s1431927620021881</a>"},"author":[{"id":"404F5528-F248-11E8-B48F-1D18A9856A87","last_name":"Fäßler","orcid":"0000-0001-7149-769X","full_name":"Fäßler, Florian","first_name":"Florian"},{"id":"38C393BE-F248-11E8-B48F-1D18A9856A87","last_name":"Dimchev","orcid":"0000-0001-8370-6161","first_name":"Georgi A","full_name":"Dimchev, Georgi A"},{"last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3904-947X","full_name":"Hodirnau, Victor-Valentin","first_name":"Victor-Valentin"},{"first_name":"Bettina","full_name":"Zens, Bettina","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","last_name":"Zens","orcid":"0000-0002-9561-1239"},{"last_name":"Möhl","first_name":"Christoph","full_name":"Möhl, Christoph"},{"full_name":"Bradke, Frank","first_name":"Frank","last_name":"Bradke"},{"orcid":"0000-0003-4790-8078","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","full_name":"Schur, Florian KM","first_name":"Florian KM"}],"language":[{"iso":"eng"}],"_id":"15286","doi":"10.1017/s1431927620021881","intvolume":"        26","day":"01","oa_version":"None","keyword":["Instrumentation"],"type":"journal_article","date_created":"2024-04-03T09:40:11Z","year":"2020","department":[{"_id":"FlSc"},{"_id":"EM-Fac"}],"page":"2518-2519","article_type":"original"},{"publication_identifier":{"issn":["1022-2588"]},"scopus_import":"1","issue":"1","article_processing_charge":"No","date_created":"2020-04-28T08:37:38Z","year":"2020","corr_author":"1","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"checksum":"fee784f15a489deb7def6ccf8c5bf8c3","content_type":"application/pdf","access_level":"open_access","file_size":579291,"date_created":"2020-06-17T10:50:13Z","relation":"main_file","file_id":"7970","creator":"dernst","date_updated":"2024-03-12T10:12:33Z","file_name":"2020_VOEB_Ernst.pdf"}],"popular_science":"1","intvolume":"        73","_id":"7687","language":[{"iso":"ger"}],"day":"28","has_accepted_license":"1","publication_status":"published","oa":1,"file_date_updated":"2024-03-12T10:12:33Z","volume":73,"date_updated":"2026-03-16T13:47:31Z","publication":"Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare","month":"04","oaworkid":1,"page":"46-59","article_type":"original","abstract":[{"text":"A working group, which was established within the Network of Repository Managers  (RepManNet),  has  dealt  with  common  certifications  for  repositories.  In addition,  current  requirements  of  the  research  funding  agencies  FWF  and  EU  were also taken into account. The Core Trust Seal was examined in more detail. For this purpose,  a  questionnaire  was  sent  to  those  organizations  that  are  already  certified with CTS in Austria. The answers were summarized and evaluated anonymously. It is recommended to go for a repository certification. Moreover, the development of a DINI certificate in Austria is strongly suggested.","lang":"eng"},{"lang":"ger","text":" Eine Arbeitsgruppe, die im Rahmen des Netzwerks für RepositorienmanagerInnen (RepManNet) entstanden ist, hat sich mit gängigen Zertifizierungen für Repositorien beschäftigt. Weiters wurden aktuelle Vorgaben der Forschungsförderer FWF und EU herangezogen. Das Core Trust Seal wurde genauer betrachtet. Hierfür  wurden jenen  Organisationen,  die  in  Österreich  bereits  mit  CTS  zertifiziert sind, ein Fragebogen übermittelt. Die Antworten wurden anonymisiert zusammengefasst und ausgewertet. Plädiert wird für eine Zertifizierung von Repositorien und die Entwicklung einer DINI-Zertifizierung in Österreich."}],"type":"journal_article","ddc":["020"],"department":[{"_id":"E-Lib"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare","citation":{"short":"D. Ernst, G. Novotny, E.M. Schönher, Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare 73 (2020) 46–59.","mla":"Ernst, Doris, et al. “(Core Trust) Seal your repository!” <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>, vol. 73, no. 1, Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2020, pp. 46–59, doi:<a href=\"https://doi.org/10.31263/voebm.v73i1.3491\">10.31263/voebm.v73i1.3491</a>.","chicago":"Ernst, Doris, Gertraud Novotny, and Eva Maria Schönher. “(Core Trust) Seal your repository!” <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2020. <a href=\"https://doi.org/10.31263/voebm.v73i1.3491\">https://doi.org/10.31263/voebm.v73i1.3491</a>.","ieee":"D. Ernst, G. Novotny, and E. M. Schönher, “(Core Trust) Seal your repository!,” <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>, vol. 73, no. 1. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, pp. 46–59, 2020.","ista":"Ernst D, Novotny G, Schönher EM. 2020. (Core Trust) Seal your repository! Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 73(1), 46–59.","apa":"Ernst, D., Novotny, G., &#38; Schönher, E. M. (2020). (Core Trust) Seal your repository! <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. <a href=\"https://doi.org/10.31263/voebm.v73i1.3491\">https://doi.org/10.31263/voebm.v73i1.3491</a>","ama":"Ernst D, Novotny G, Schönher EM. (Core Trust) Seal your repository! <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>. 2020;73(1):46-59. doi:<a href=\"https://doi.org/10.31263/voebm.v73i1.3491\">10.31263/voebm.v73i1.3491</a>"},"author":[{"full_name":"Ernst, Doris","first_name":"Doris","id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","last_name":"Ernst","orcid":"0000-0002-2354-0195"},{"last_name":"Novotny","first_name":"Gertraud","full_name":"Novotny, Gertraud"},{"last_name":"Schönher","full_name":"Schönher, Eva Maria","first_name":"Eva Maria"}],"doi":"10.31263/voebm.v73i1.3491","oa_version":"Published Version","status":"public","external_id":{"oaworkid":["W3021112752"]},"title":"(Core Trust) Seal your repository!","date_published":"2020-04-28T00:00:00Z"},{"acknowledgement":"J.T.-G. and G.Á.-P. acknowledge support through the Severo Ochoa Program from the Government of the Principality of Asturias (nos. PA-18-PF-BP17-126 and PA-20-PF-BP19-053, respectively). J.M.-S. acknowledges finantial support from the Clarín Programme from the Government of the Principality of Asturias and a Marie Curie-COFUND grant (PA-18-ACB17-29) and the Ramón y Cajal Program from the Government of Spain (RYC2018-026196-I). K.C., X.P.A.G., H.V. and M.H.B. acknowledge the Air Force Office of Scientific Research (AFOSR) grant no. FA 9550-18-1-0030 for funding support. I.E. acknowledges financial support from the Spanish Ministry of Economy and Competitiveness (grant no. FIS2016-76617-P). A.Y.N. acknowledges the Spanish Ministry of Science, Innovation and Universities (national project no. MAT2017-88358-C3-3-R) and the Basque Government (grant no. IT1164-19). Q.B. acknowledges the support from Australian Research Council (grant nos. FT150100450, IH150100006 and CE170100039). R.H. acknowledges support from the Spanish Ministry of Economy, Industry, and Competitiveness (national project RTI2018-094830-B-100 and the Project MDM-2016-0618 of the María de Maeztu Units of Excellence Program) and the Basque Goverment (grant no. IT1164-19). P.A.-G. acknowledges support from the European Research Council under starting grant no. 715496, 2DNANOPTICA.","oa":1,"publication_status":"published","date_updated":"2025-04-23T14:24:58Z","volume":19,"arxiv":1,"quality_controlled":"1","month":"09","publication":"Nature Materials","OA_type":"green","page":"964–968","article_type":"original","abstract":[{"text":"Phonon polaritons—light coupled to lattice vibrations—in polar van der Waals crystals are promising candidates for controlling the flow of energy on the nanoscale due to their strong field confinement, anisotropic propagation and ultra-long lifetime in the picosecond range1,2,3,4,5. However, the lack of tunability of their narrow and material-specific spectral range—the Reststrahlen band—severely limits their technological implementation. Here, we demonstrate that intercalation of Na atoms in the van der Waals semiconductor α-V2O5 enables a broad spectral shift of Reststrahlen bands, and that the phonon polaritons excited show ultra-low losses (lifetime of 4 ± 1 ps), similar to phonon polaritons in a non-intercalated crystal (lifetime of 6 ± 1 ps). We expect our intercalation method to be applicable to other van der Waals crystals, opening the door for the use of phonon polaritons in broad spectral bands in the mid-infrared domain.","lang":"eng"}],"type":"journal_article","department":[{"_id":"NanoFab"}],"doi":"10.1038/s41563-020-0665-0","pmid":1,"OA_place":"repository","oa_version":"Preprint","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"short":"J. Taboada-Gutiérrez, G. Álvarez-Pérez, J. Duan, W. Ma, K. Crowley, I. Prieto Gonzalez, A. Bylinkin, M. Autore, H. Volkova, K. Kimura, T. Kimura, M.H. Berger, S. Li, Q. Bao, X.P.A. Gao, I. Errea, A.Y. Nikitin, R. Hillenbrand, J. Martín-Sánchez, P. Alonso-González, Nature Materials 19 (2020) 964–968.","mla":"Taboada-Gutiérrez, Javier, et al. “Broad Spectral Tuning of Ultra-Low-Loss Polaritons in a van Der Waals Crystal by Intercalation.” <i>Nature Materials</i>, vol. 19, Springer Nature, 2020, pp. 964–968, doi:<a href=\"https://doi.org/10.1038/s41563-020-0665-0\">10.1038/s41563-020-0665-0</a>.","chicago":"Taboada-Gutiérrez, Javier, Gonzalo Álvarez-Pérez, Jiahua Duan, Weiliang Ma, Kyle Crowley, Ivan Prieto Gonzalez, Andrei Bylinkin, et al. “Broad Spectral Tuning of Ultra-Low-Loss Polaritons in a van Der Waals Crystal by Intercalation.” <i>Nature Materials</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41563-020-0665-0\">https://doi.org/10.1038/s41563-020-0665-0</a>.","apa":"Taboada-Gutiérrez, J., Álvarez-Pérez, G., Duan, J., Ma, W., Crowley, K., Prieto Gonzalez, I., … Alonso-González, P. (2020). Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation. <i>Nature Materials</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41563-020-0665-0\">https://doi.org/10.1038/s41563-020-0665-0</a>","ista":"Taboada-Gutiérrez J, Álvarez-Pérez G, Duan J, Ma W, Crowley K, Prieto Gonzalez I, Bylinkin A, Autore M, Volkova H, Kimura K, Kimura T, Berger MH, Li S, Bao Q, Gao XPA, Errea I, Nikitin AY, Hillenbrand R, Martín-Sánchez J, Alonso-González P. 2020. Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation. Nature Materials. 19, 964–968.","ieee":"J. Taboada-Gutiérrez <i>et al.</i>, “Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation,” <i>Nature Materials</i>, vol. 19. Springer Nature, pp. 964–968, 2020.","ama":"Taboada-Gutiérrez J, Álvarez-Pérez G, Duan J, et al. Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation. <i>Nature Materials</i>. 2020;19:964–968. doi:<a href=\"https://doi.org/10.1038/s41563-020-0665-0\">10.1038/s41563-020-0665-0</a>"},"publisher":"Springer Nature","author":[{"first_name":"Javier","full_name":"Taboada-Gutiérrez, Javier","last_name":"Taboada-Gutiérrez"},{"last_name":"Álvarez-Pérez","first_name":"Gonzalo","full_name":"Álvarez-Pérez, Gonzalo"},{"last_name":"Duan","full_name":"Duan, Jiahua","first_name":"Jiahua"},{"last_name":"Ma","full_name":"Ma, Weiliang","first_name":"Weiliang"},{"first_name":"Kyle","full_name":"Crowley, Kyle","last_name":"Crowley"},{"last_name":"Prieto Gonzalez","id":"2A307FE2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7370-5357","first_name":"Ivan","full_name":"Prieto Gonzalez, Ivan"},{"last_name":"Bylinkin","full_name":"Bylinkin, Andrei","first_name":"Andrei"},{"last_name":"Autore","first_name":"Marta","full_name":"Autore, Marta"},{"last_name":"Volkova","first_name":"Halyna","full_name":"Volkova, Halyna"},{"last_name":"Kimura","full_name":"Kimura, Kenta","first_name":"Kenta"},{"full_name":"Kimura, Tsuyoshi","first_name":"Tsuyoshi","last_name":"Kimura"},{"first_name":"M. H.","full_name":"Berger, M. H.","last_name":"Berger"},{"last_name":"Li","full_name":"Li, Shaojuan","first_name":"Shaojuan"},{"full_name":"Bao, Qiaoliang","first_name":"Qiaoliang","last_name":"Bao"},{"last_name":"Gao","first_name":"Xuan P.A.","full_name":"Gao, Xuan P.A."},{"first_name":"Ion","full_name":"Errea, Ion","last_name":"Errea"},{"last_name":"Nikitin","full_name":"Nikitin, Alexey Y.","first_name":"Alexey Y."},{"last_name":"Hillenbrand","full_name":"Hillenbrand, Rainer","first_name":"Rainer"},{"full_name":"Martín-Sánchez, Javier","first_name":"Javier","last_name":"Martín-Sánchez"},{"full_name":"Alonso-González, Pablo","first_name":"Pablo","last_name":"Alonso-González"}],"external_id":{"arxiv":["2501.08705"],"pmid":["32284598"],"isi":["000526218500004"]},"date_published":"2020-09-01T00:00:00Z","title":"Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation","status":"public","scopus_import":"1","publication_identifier":{"eissn":["1476-4660"],"issn":["1476-1122"]},"article_processing_charge":"No","main_file_link":[{"open_access":"1","url":"https://doi.org/10.48550/arXiv.2501.08705"}],"isi":1,"year":"2020","date_created":"2020-05-03T22:00:49Z","_id":"7792","language":[{"iso":"eng"}],"intvolume":"        19","day":"01"},{"date_created":"2020-05-17T22:00:44Z","year":"2020","isi":1,"intvolume":"        20","_id":"7864","language":[{"iso":"eng"}],"day":"01","scopus_import":"1","issue":"3","publication_identifier":{"eissn":["14736322"]},"article_processing_charge":"No","type":"journal_article","abstract":[{"lang":"eng","text":"Purpose of review: Cancer is one of the leading causes of death and the incidence rates are constantly rising. The heterogeneity of tumors poses a big challenge for the treatment of the disease and natural antibodies additionally affect disease progression. The introduction of engineered mAbs for anticancer immunotherapies has substantially improved progression-free and overall survival of cancer patients, but little efforts have been made to exploit other antibody isotypes than IgG.\r\nRecent findings: In order to improve these therapies, ‘next-generation antibodies’ were engineered to enhance a specific feature of classical antibodies and form a group of highly effective and precise therapy compounds. Advanced antibody approaches include among others antibody-drug conjugates, glyco-engineered and Fc-engineered antibodies, antibody fragments, radioimmunotherapy compounds, bispecific antibodies and alternative (non-IgG) immunoglobulin classes, especially IgE.\r\nSummary: The current review describes solutions for the needs of next-generation antibody therapies through different approaches. Careful selection of the best-suited engineering methodology is a key factor in developing personalized, more specific and more efficient mAbs against cancer to improve the outcomes of cancer patients. We highlight here the large evidence of IgE exploiting a highly cytotoxic effector arm as potential next-generation anticancer immunotherapy."}],"department":[{"_id":"Bio"}],"page":"282-289","article_type":"original","external_id":{"isi":["000561358300010"]},"title":"Precision medicine in clinical oncology: the journey from IgG antibody to IgE","date_published":"2020-06-01T00:00:00Z","status":"public","doi":"10.1097/ACI.0000000000000637","oa_version":"None","citation":{"ista":"Singer J, Singer J, Jensen-Jarolim E. 2020. Precision medicine in clinical oncology: the journey from IgG antibody to IgE. Current opinion in allergy and clinical immunology. 20(3), 282–289.","apa":"Singer, J., Singer, J., &#38; Jensen-Jarolim, E. (2020). Precision medicine in clinical oncology: the journey from IgG antibody to IgE. <i>Current Opinion in Allergy and Clinical Immunology</i>. Wolters Kluwer. <a href=\"https://doi.org/10.1097/ACI.0000000000000637\">https://doi.org/10.1097/ACI.0000000000000637</a>","ieee":"J. Singer, J. Singer, and E. Jensen-Jarolim, “Precision medicine in clinical oncology: the journey from IgG antibody to IgE,” <i>Current opinion in allergy and clinical immunology</i>, vol. 20, no. 3. Wolters Kluwer, pp. 282–289, 2020.","ama":"Singer J, Singer J, Jensen-Jarolim E. Precision medicine in clinical oncology: the journey from IgG antibody to IgE. <i>Current opinion in allergy and clinical immunology</i>. 2020;20(3):282-289. doi:<a href=\"https://doi.org/10.1097/ACI.0000000000000637\">10.1097/ACI.0000000000000637</a>","mla":"Singer, Judit, et al. “Precision Medicine in Clinical Oncology: The Journey from IgG Antibody to IgE.” <i>Current Opinion in Allergy and Clinical Immunology</i>, vol. 20, no. 3, Wolters Kluwer, 2020, pp. 282–89, doi:<a href=\"https://doi.org/10.1097/ACI.0000000000000637\">10.1097/ACI.0000000000000637</a>.","short":"J. Singer, J. Singer, E. Jensen-Jarolim, Current Opinion in Allergy and Clinical Immunology 20 (2020) 282–289.","chicago":"Singer, Judit, Josef Singer, and Erika Jensen-Jarolim. “Precision Medicine in Clinical Oncology: The Journey from IgG Antibody to IgE.” <i>Current Opinion in Allergy and Clinical Immunology</i>. Wolters Kluwer, 2020. <a href=\"https://doi.org/10.1097/ACI.0000000000000637\">https://doi.org/10.1097/ACI.0000000000000637</a>."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"Wolters Kluwer","author":[{"id":"36432834-F248-11E8-B48F-1D18A9856A87","last_name":"Singer","orcid":"0000-0002-8777-3502","full_name":"Singer, Judit","first_name":"Judit"},{"first_name":"Josef","full_name":"Singer, Josef","last_name":"Singer"},{"last_name":"Jensen-Jarolim","full_name":"Jensen-Jarolim, Erika","first_name":"Erika"}],"publication_status":"published","quality_controlled":"1","month":"06","publication":"Current opinion in allergy and clinical immunology","date_updated":"2023-08-21T06:28:52Z","volume":20},{"file":[{"file_name":"2020_JCellBiol_Kopf.pdf","date_updated":"2020-11-24T13:25:13Z","creator":"dernst","file_id":"8801","success":1,"relation":"main_file","file_size":7536712,"date_created":"2020-11-24T13:25:13Z","access_level":"open_access","content_type":"application/pdf","checksum":"cb0b9c77842ae1214caade7b77e4d82d"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"corr_author":"1","day":"01","language":[{"iso":"eng"}],"_id":"7875","intvolume":"       219","date_created":"2020-05-24T22:00:56Z","year":"2020","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"PreCl"}],"isi":1,"project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FP7"},{"name":"Cellular Navigation Along Spatial Gradients","call_identifier":"H2020","_id":"25FE9508-B435-11E9-9278-68D0E5697425","grant_number":"724373"},{"call_identifier":"FWF","name":"Mechanical adaptation of lamellipodial actin","_id":"26018E70-B435-11E9-9278-68D0E5697425","grant_number":"P29911"},{"name":"Nano-Analytics of Cellular Systems","call_identifier":"FWF","grant_number":"W1250-B20","_id":"252C3B08-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"},{"name":"Molecular and system level view of immune cell migration","grant_number":"ALTF 1396-2014","_id":"25A48D24-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"No","ec_funded":1,"publication_identifier":{"eissn":["1540-8140"]},"issue":"6","scopus_import":"1","status":"public","article_number":"e201907154","title":"Microtubules control cellular shape and coherence in amoeboid migrating cells","date_published":"2020-06-01T00:00:00Z","external_id":{"isi":["000538141100020"],"pmid":["32379884"]},"author":[{"first_name":"Aglaja","full_name":"Kopf, Aglaja","orcid":"0000-0002-2187-6656","last_name":"Kopf","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Renkawitz, Jörg","first_name":"Jörg","last_name":"Renkawitz","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2856-3369"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert"},{"first_name":"Irute","full_name":"Girkontaite, Irute","last_name":"Girkontaite"},{"last_name":"Tedford","first_name":"Kerry","full_name":"Tedford, Kerry"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609","first_name":"Jack","full_name":"Merrin, Jack"},{"first_name":"Oliver","full_name":"Thorn-Seshold, Oliver","last_name":"Thorn-Seshold"},{"last_name":"Trauner","id":"E8F27F48-3EBA-11E9-92A1-B709E6697425","first_name":"Dirk","full_name":"Trauner, Dirk"},{"first_name":"Hans","full_name":"Häcker, Hans","last_name":"Häcker"},{"first_name":"Klaus Dieter","full_name":"Fischer, Klaus Dieter","last_name":"Fischer"},{"last_name":"Kiermaier","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6165-5738","first_name":"Eva","full_name":"Kiermaier, Eva"},{"first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179"}],"citation":{"ista":"Kopf A, Renkawitz J, Hauschild R, Girkontaite I, Tedford K, Merrin J, Thorn-Seshold O, Trauner D, Häcker H, Fischer KD, Kiermaier E, Sixt MK. 2020. Microtubules control cellular shape and coherence in amoeboid migrating cells. The Journal of Cell Biology. 219(6), e201907154.","apa":"Kopf, A., Renkawitz, J., Hauschild, R., Girkontaite, I., Tedford, K., Merrin, J., … Sixt, M. K. (2020). Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>","ieee":"A. Kopf <i>et al.</i>, “Microtubules control cellular shape and coherence in amoeboid migrating cells,” <i>The Journal of Cell Biology</i>, vol. 219, no. 6. Rockefeller University Press, 2020.","ama":"Kopf A, Renkawitz J, Hauschild R, et al. Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. 2020;219(6). doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>","mla":"Kopf, Aglaja, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>, vol. 219, no. 6, e201907154, Rockefeller University Press, 2020, doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>.","short":"A. Kopf, J. Renkawitz, R. Hauschild, I. Girkontaite, K. Tedford, J. Merrin, O. Thorn-Seshold, D. Trauner, H. Häcker, K.D. Fischer, E. Kiermaier, M.K. Sixt, The Journal of Cell Biology 219 (2020).","chicago":"Kopf, Aglaja, Jörg Renkawitz, Robert Hauschild, Irute Girkontaite, Kerry Tedford, Jack Merrin, Oliver Thorn-Seshold, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>. Rockefeller University Press, 2020. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>."},"publisher":"Rockefeller University Press","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","pmid":1,"doi":"10.1083/jcb.201907154","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"ddc":["570"],"abstract":[{"lang":"eng","text":"Cells navigating through complex tissues face a fundamental challenge: while multiple protrusions explore different paths, the cell needs to avoid entanglement. How a cell surveys and then corrects its own shape is poorly understood. Here, we demonstrate that spatially distinct microtubule dynamics regulate amoeboid cell migration by locally promoting the retraction of protrusions. In migrating dendritic cells, local microtubule depolymerization within protrusions remote from the microtubule organizing center triggers actomyosin contractility controlled by RhoA and its exchange factor Lfc. Depletion of Lfc leads to aberrant myosin localization, thereby causing two effects that rate-limit locomotion: (1) impaired cell edge coordination during path finding and (2) defective adhesion resolution. Compromised shape control is particularly hindering in geometrically complex microenvironments, where it leads to entanglement and ultimately fragmentation of the cell body. We thus demonstrate that microtubules can act as a proprioceptive device: they sense cell shape and control actomyosin retraction to sustain cellular coherence."}],"type":"journal_article","article_type":"original","publication":"The Journal of Cell Biology","month":"06","quality_controlled":"1","volume":219,"date_updated":"2025-04-14T13:10:03Z","publication_status":"published","file_date_updated":"2020-11-24T13:25:13Z","oa":1,"has_accepted_license":"1","acknowledgement":"The authors thank the Scientific Service Units (Life Sciences, Bioimaging, Preclinical) of the Institute of Science and Technology Austria for excellent support. This work was funded by the European Research Council (ERC StG 281556 and CoG 724373), two grants from the Austrian\r\nScience Fund (FWF; P29911 and DK Nanocell W1250-B20 to M. Sixt) and by the German Research Foundation (DFG SFB1032 project B09) to O. Thorn-Seshold and D. Trauner. J. Renkawitz was supported by ISTFELLOW funding from the People Program (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under the Research Executive Agency grant agreement (291734) and a European Molecular Biology Organization long-term fellowship (ALTF 1396-2014) co-funded by the European Commission (LTFCOFUND2013, GA-2013-609409), E. Kiermaier by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy—EXC 2151—390873048, and H. Hacker by the American Lebanese Syrian Associated ¨Charities. K.-D. Fischer was supported by the Analysis, Imaging and Modelling of Neuronal and Inflammatory Processes graduate school funded by the Ministry of Economics, Science, and Digitisation of the State Saxony-Anhalt and by the European Funds for Social and Regional Development."},{"department":[{"_id":"Bio"}],"date_created":"2020-07-28T16:24:37Z","year":"2020","type":"software","date_published":"2020-08-24T00:00:00Z","title":"Amplified centrosomes in dendritic cells promote immune cell effector functions","status":"public","day":"24","_id":"8181","doi":"10.15479/AT:ISTA:8181","author":[{"orcid":"0000-0001-9843-3522","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","first_name":"Robert"}],"tmp":{"short":"3-Clause BSD","name":"The 3-Clause BSD License","legal_code_url":"https://opensource.org/licenses/BSD-3-Clause"},"file":[{"relation":"main_file","date_created":"2020-08-24T15:43:49Z","file_size":6577,"access_level":"open_access","checksum":"878c60885ce30afb59a884dd5eef451c","content_type":"text/plain","file_name":"centriolesDistance.m","date_updated":"2020-08-24T15:43:49Z","file_id":"8290","success":1,"creator":"rhauschild"},{"file_name":"goTracking.m","success":1,"file_id":"8291","creator":"rhauschild","date_updated":"2020-08-24T15:43:52Z","file_size":2680,"date_created":"2020-08-24T15:43:52Z","relation":"main_file","access_level":"open_access","checksum":"5a93ac7be2b66b28e4bd8b113ee6aade","content_type":"text/plain"}],"citation":{"chicago":"Hauschild, Robert. “Amplified Centrosomes in Dendritic Cells Promote Immune Cell Effector Functions.” IST Austria, 2020. <a href=\"https://doi.org/10.15479/AT:ISTA:8181\">https://doi.org/10.15479/AT:ISTA:8181</a>.","short":"R. Hauschild, (2020).","mla":"Hauschild, Robert. <i>Amplified Centrosomes in Dendritic Cells Promote Immune Cell Effector Functions</i>. IST Austria, 2020, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8181\">10.15479/AT:ISTA:8181</a>.","ama":"Hauschild R. Amplified centrosomes in dendritic cells promote immune cell effector functions. 2020. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8181\">10.15479/AT:ISTA:8181</a>","ieee":"R. Hauschild, “Amplified centrosomes in dendritic cells promote immune cell effector functions.” IST Austria, 2020.","apa":"Hauschild, R. (2020). Amplified centrosomes in dendritic cells promote immune cell effector functions. IST Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:8181\">https://doi.org/10.15479/AT:ISTA:8181</a>","ista":"Hauschild R. 2020. Amplified centrosomes in dendritic cells promote immune cell effector functions, IST Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:8181\">10.15479/AT:ISTA:8181</a>."},"corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"IST Austria","file_date_updated":"2020-08-24T15:43:52Z","oa":1,"has_accepted_license":"1","license":"https://opensource.org/licenses/BSD-3-Clause","month":"08","date_updated":"2024-10-09T21:05:14Z"},{"scopus_import":"1","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","issue":"6","publication_identifier":{"issn":["0896-6273"]},"ec_funded":1,"article_processing_charge":"No","project":[{"call_identifier":"H2020","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","grant_number":"692692","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"Synaptic communication in neuronal microcircuits","grant_number":"Z00312","_id":"25C5A090-B435-11E9-9278-68D0E5697425"}],"isi":1,"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"ScienComp"},{"_id":"PreCl"}],"date_created":"2020-08-14T09:36:05Z","year":"2020","intvolume":"       107","_id":"8261","language":[{"iso":"eng"}],"day":"23","related_material":{"link":[{"url":"https://ist.ac.at/en/news/the-bouncer-in-the-brain/","relation":"press_release","description":"News on IST Website"}]},"corr_author":"1","tmp":{"short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"file":[{"relation":"main_file","file_size":3011120,"date_created":"2020-12-04T09:29:21Z","checksum":"44a5960fc083a4cb3488d22224859fdc","content_type":"application/pdf","access_level":"open_access","file_name":"2020_Neuron_Zhang.pdf","date_updated":"2020-12-04T09:29:21Z","creator":"dernst","file_id":"8920","success":1}],"acknowledgement":"This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement 692692, P.J.) and the Fond zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award, P.J.). We thank Gyorgy Buzsáki, Jozsef Csicsvari, Juan Ramirez Villegas, and Federico Stella for commenting on earlier versions of this manuscript. We also thank Katie Bittner, Michael Brecht, Albert Lee, Jeffery Magee, and Alejandro Pernía-Andrade for sharing expertise in in vivo patch-clamp recording. We are grateful to Florian Marr for cell labeling, cell reconstruction, and technical assistance; Ben Suter for helpful discussions; Christina Altmutter for technical support; Eleftheria Kralli-Beller for manuscript editing; and Todor Asenov (Machine Shop) for device construction. We also thank the Scientific Service Units (SSUs) of IST Austria (Machine Shop, Scientific Computing, and Preclinical Facility) for efficient support.","has_accepted_license":"1","oa":1,"file_date_updated":"2020-12-04T09:29:21Z","publication_status":"published","date_updated":"2025-04-15T08:29:03Z","volume":107,"quality_controlled":"1","month":"09","publication":"Neuron","page":"1212-1225","article_type":"original","type":"journal_article","abstract":[{"lang":"eng","text":"Dentate gyrus granule cells (GCs) connect the entorhinal cortex to the hippocampal CA3 region, but how they process spatial information remains enigmatic. To examine the role of GCs in spatial coding, we measured excitatory postsynaptic potentials (EPSPs) and action potentials (APs) in head-fixed mice running on a linear belt. Intracellular recording from morphologically identified GCs revealed that most cells were active, but activity level varied over a wide range. Whereas only ∼5% of GCs showed spatially tuned spiking, ∼50% received spatially tuned input. Thus, the GC population broadly encodes spatial information, but only a subset relays this information to the CA3 network. Fourier analysis indicated that GCs received conjunctive place-grid-like synaptic input, suggesting code conversion in single neurons. GC firing was correlated with dendritic complexity and intrinsic excitability, but not extrinsic excitatory input or dendritic cable properties. Thus, functional maturation may control input-output transformation and spatial code conversion."}],"department":[{"_id":"PeJo"},{"_id":"ScienComp"}],"ddc":["570"],"doi":"10.1016/j.neuron.2020.07.006","pmid":1,"oa_version":"Published Version","citation":{"chicago":"Zhang, Xiaomin, Alois Schlögl, and Peter M Jonas. “Selective Routing of Spatial Information Flow from Input to Output in Hippocampal Granule Cells.” <i>Neuron</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.neuron.2020.07.006\">https://doi.org/10.1016/j.neuron.2020.07.006</a>.","short":"X. Zhang, A. Schlögl, P.M. Jonas, Neuron 107 (2020) 1212–1225.","mla":"Zhang, Xiaomin, et al. “Selective Routing of Spatial Information Flow from Input to Output in Hippocampal Granule Cells.” <i>Neuron</i>, vol. 107, no. 6, Elsevier, 2020, pp. 1212–25, doi:<a href=\"https://doi.org/10.1016/j.neuron.2020.07.006\">10.1016/j.neuron.2020.07.006</a>.","ama":"Zhang X, Schlögl A, Jonas PM. Selective routing of spatial information flow from input to output in hippocampal granule cells. <i>Neuron</i>. 2020;107(6):1212-1225. doi:<a href=\"https://doi.org/10.1016/j.neuron.2020.07.006\">10.1016/j.neuron.2020.07.006</a>","ista":"Zhang X, Schlögl A, Jonas PM. 2020. Selective routing of spatial information flow from input to output in hippocampal granule cells. Neuron. 107(6), 1212–1225.","apa":"Zhang, X., Schlögl, A., &#38; Jonas, P. M. (2020). Selective routing of spatial information flow from input to output in hippocampal granule cells. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2020.07.006\">https://doi.org/10.1016/j.neuron.2020.07.006</a>","ieee":"X. Zhang, A. Schlögl, and P. M. Jonas, “Selective routing of spatial information flow from input to output in hippocampal granule cells,” <i>Neuron</i>, vol. 107, no. 6. Elsevier, pp. 1212–1225, 2020."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"Elsevier","author":[{"last_name":"Zhang","id":"423EC9C2-F248-11E8-B48F-1D18A9856A87","first_name":"Xiaomin","full_name":"Zhang, Xiaomin"},{"full_name":"Schlögl, Alois","first_name":"Alois","orcid":"0000-0002-5621-8100","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl"},{"first_name":"Peter M","full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"external_id":{"pmid":["32763145"],"isi":["000579698700009"]},"title":"Selective routing of spatial information flow from input to output in hippocampal granule cells","date_published":"2020-09-23T00:00:00Z","status":"public"},{"has_accepted_license":"1","file_date_updated":"2020-09-08T14:26:33Z","oa":1,"date_updated":"2024-10-09T21:05:14Z","month":"09","department":[{"_id":"Bio"}],"date_created":"2020-08-25T12:52:48Z","year":"2020","ddc":["570"],"type":"software","abstract":[{"text":"Automated root growth analysis and tracking of root tips. ","lang":"eng"}],"tmp":{"short":"3-Clause BSD","name":"The 3-Clause BSD License","legal_code_url":"https://opensource.org/licenses/BSD-3-Clause"},"file":[{"access_level":"open_access","checksum":"108352149987ac6f066e4925bd56e35e","content_type":"text/plain","relation":"main_file","date_created":"2020-09-08T14:26:31Z","file_size":882,"date_updated":"2020-09-08T14:26:31Z","creator":"rhauschild","file_id":"8346","success":1,"file_name":"readme.txt"},{"relation":"main_file","file_size":246121,"date_created":"2020-09-08T14:26:33Z","checksum":"ffd6c643b28e0cc7c6d0060a18a7e8ea","content_type":"application/octet-stream","access_level":"open_access","file_name":"RGtracker.mlappinstall","date_updated":"2020-09-08T14:26:33Z","file_id":"8347","creator":"rhauschild","success":1}],"author":[{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","publisher":"IST Austria","citation":{"ama":"Hauschild R. RGtracker. 2020. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8294\">10.15479/AT:ISTA:8294</a>","ista":"Hauschild R. 2020. RGtracker, IST Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:8294\">10.15479/AT:ISTA:8294</a>.","apa":"Hauschild, R. (2020). RGtracker. IST Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:8294\">https://doi.org/10.15479/AT:ISTA:8294</a>","ieee":"R. Hauschild, “RGtracker.” IST Austria, 2020.","chicago":"Hauschild, Robert. “RGtracker.” IST Austria, 2020. <a href=\"https://doi.org/10.15479/AT:ISTA:8294\">https://doi.org/10.15479/AT:ISTA:8294</a>.","mla":"Hauschild, Robert. <i>RGtracker</i>. IST Austria, 2020, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8294\">10.15479/AT:ISTA:8294</a>.","short":"R. Hauschild, (2020)."},"day":"10","_id":"8294","doi":"10.15479/AT:ISTA:8294","status":"public","date_published":"2020-09-10T00:00:00Z","title":"RGtracker"},{"scopus_import":"1","issue":"6","publication_identifier":{"eissn":["1478-3975"]},"article_processing_charge":"Yes (via OA deal)","date_created":"2020-10-04T22:01:35Z","year":"2020","isi":1,"language":[{"iso":"eng"}],"_id":"8597","intvolume":"        17","day":"23","corr_author":"1","file":[{"relation":"main_file","date_created":"2020-10-05T13:53:59Z","file_size":1667111,"checksum":"fec9bdd355ed349f09990faab20838a7","content_type":"application/pdf","access_level":"open_access","file_name":"2020_PhysBio_Merrin.pdf","date_updated":"2020-10-05T13:53:59Z","file_id":"8609","success":1,"creator":"dernst"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"oa":1,"file_date_updated":"2020-10-05T13:53:59Z","publication_status":"published","has_accepted_license":"1","acknowledgement":"I would especially like to thank Michael Sixt for encouraging me to think about these problems while working at home due to restrictions in place. I want to thank Nick Barton, Katka Bodova, Matthew Robinson, Simon Rella, Federico Sau, Ivan Prieto, and Pradeep Kumar for useful discussions.","month":"09","quality_controlled":"1","publication":"Physical Biology","date_updated":"2026-04-02T14:29:42Z","volume":17,"abstract":[{"lang":"eng","text":"Error analysis and data visualization of positive COVID-19 cases in 27 countries have been performed up to August 8, 2020. This survey generally observes a progression from early exponential growth transitioning to an intermediate power-law growth phase, as recently suggested by Ziff and Ziff. The occurrence of logistic growth after the power-law phase with lockdowns or social distancing may be described as an effect of avoidance. A visualization of the power-law growth exponent over short time windows is qualitatively similar to the Bhatia visualization for pandemic progression. Visualizations like these can indicate the onset of second waves and may influence social policy."}],"type":"journal_article","ddc":["510","570"],"department":[{"_id":"NanoFab"}],"article_type":"original","external_id":{"isi":["000575539700001"]},"article_number":"065005","title":"Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide","date_published":"2020-09-23T00:00:00Z","status":"public","doi":"10.1088/1478-3975/abb2db","oa_version":"Published Version","citation":{"chicago":"Merrin, Jack. “Differences in Power Law Growth over Time and Indicators of COVID-19 Pandemic Progression Worldwide.” <i>Physical Biology</i>. IOP Publishing, 2020. <a href=\"https://doi.org/10.1088/1478-3975/abb2db\">https://doi.org/10.1088/1478-3975/abb2db</a>.","short":"J. Merrin, Physical Biology 17 (2020).","mla":"Merrin, Jack. “Differences in Power Law Growth over Time and Indicators of COVID-19 Pandemic Progression Worldwide.” <i>Physical Biology</i>, vol. 17, no. 6, 065005, IOP Publishing, 2020, doi:<a href=\"https://doi.org/10.1088/1478-3975/abb2db\">10.1088/1478-3975/abb2db</a>.","ama":"Merrin J. Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide. <i>Physical Biology</i>. 2020;17(6). doi:<a href=\"https://doi.org/10.1088/1478-3975/abb2db\">10.1088/1478-3975/abb2db</a>","apa":"Merrin, J. (2020). Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide. <i>Physical Biology</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/1478-3975/abb2db\">https://doi.org/10.1088/1478-3975/abb2db</a>","ista":"Merrin J. 2020. Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide. Physical Biology. 17(6), 065005.","ieee":"J. Merrin, “Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide,” <i>Physical Biology</i>, vol. 17, no. 6. IOP Publishing, 2020."},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publisher":"IOP Publishing","author":[{"full_name":"Merrin, Jack","first_name":"Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609"}]},{"abstract":[{"text":"A look at international activities on Open Science reveals a broad spectrum from individual institutional policies to national action plans. The present Recommendations for a National Open Science Strategy in Austria are based on these international initiatives and present practical considerations for their coordinated implementation with regard to strategic developments in research, technology and innovation (RTI) in Austria until 2030. They are addressed to all relevant actors in the RTI system, in particular to Research Performing Organisations, Research Funding Organisations, Research Policy, memory institutions such as Libraries and Researchers. The recommendation paper was developed from 2018 to 2020 by the OANA working group \"Open Science Strategy\" and published for the first time in spring 2020 for a public consultation. The now available final version of the recommendation document, which contains feedback and comments from the consultation, is intended to provide an impetus for further discussion and implementation of Open Science in Austria and serves as a contribution and basis for a potential national Open Science Strategy in Austria. The document builds on the diverse expertise of the authors (academia, administration, library and archive, information technology, science policy, funding system, etc.) and reflects their personal experiences and opinions.","lang":"eng"},{"lang":"ger","text":"Der Blick auf internationale Aktivitäten zu Open Science zeigt ein breites Spektrum von einzelnen institutionellen Policies bis hin zu nationalen Aktionsplänen. Die vorliegenden Empfehlungen für eine nationale Open Science Strategie in Österreich orientieren sich an diesen internationalen Initiativen und stellen praktische Überlegungen für ihre koordinierte Implementierung im Hinblick auf strategische Entwicklungen in Forschung, Technologie und Innovation (FTI) bis 2030 in Österreich dar. Dabei richten sie sich an alle relevanten Akteur*innen im FTI System, im Besonderen an Forschungsstätten, Forschungsförderer, Forschungspolitik, Gedächtnisinstitutionen wie Bibliotheken und Wissenschafter*innen. Das Empfehlungspapier wurde von 2018 bis 2020 von der OANA-Arbeitsgruppe \"Open Science Strategie\" entwickelt und im Frühling 2020 das erste Mal für eine öffentliche Konsultation veröffentlicht. Die nun vorliegende finale Version des Empfehlungsdokuments, die Feedback und Kommentare aus der Konsultation enthält, soll ein Anstoß für die weitere Diskussion und Umsetzung von Open Science in Österreich sein und als Beitrag und Grundlage einer potentiellen nationalen Open Science Strategie in Österreich dienen. Das Dokument baut auf der vielfältigen Expertise der Autor*innen auf (Wissenschaft, Administration, Bibliothek und Archiv, Informationstechnologie, Wissenschaftspolitik, Förderwesen etc.) und spiegelt deren persönliche Erfahrungen und Meinung wider."}],"type":"working_paper","date_created":"2020-10-23T09:08:28Z","year":"2020","department":[{"_id":"E-Lib"}],"ddc":["020"],"page":"36","status":"public","title":"Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations for a National Open Science Strategy in Austria","date_published":"2020-10-21T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Mayer, Katja, Katharina Rieck, Stefan Reichmann, Patrick Danowski, Anton Graschopf, Thomas König, Peter Kraker, et al. <i>Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations for a National Open Science Strategy in Austria</i>. OANA, 2020. <a href=\"https://doi.org/10.5281/ZENODO.4109242\">https://doi.org/10.5281/ZENODO.4109242</a>.","mla":"Mayer, Katja, et al. <i>Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations for a National Open Science Strategy in Austria</i>. OANA, 2020, doi:<a href=\"https://doi.org/10.5281/ZENODO.4109242\">10.5281/ZENODO.4109242</a>.","short":"K. Mayer, K. Rieck, S. Reichmann, P. Danowski, A. Graschopf, T. König, P. Kraker, P. Lehner, F. Reckling, T. Ross-Hellauer, D. Spichtinger, M. Tzatzanis, S. Schürz, Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations for a National Open Science Strategy in Austria, OANA, 2020.","ama":"Mayer K, Rieck K, Reichmann S, et al. <i>Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations for a National Open Science Strategy in Austria</i>. OANA; 2020. doi:<a href=\"https://doi.org/10.5281/ZENODO.4109242\">10.5281/ZENODO.4109242</a>","ieee":"K. Mayer <i>et al.</i>, <i>Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations for a National Open Science Strategy in Austria</i>. OANA, 2020.","apa":"Mayer, K., Rieck, K., Reichmann, S., Danowski, P., Graschopf, A., König, T., … Schürz, S. (2020). <i>Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations for a National Open Science Strategy in Austria</i>. OANA. <a href=\"https://doi.org/10.5281/ZENODO.4109242\">https://doi.org/10.5281/ZENODO.4109242</a>","ista":"Mayer K, Rieck K, Reichmann S, Danowski P, Graschopf A, König T, Kraker P, Lehner P, Reckling F, Ross-Hellauer T, Spichtinger D, Tzatzanis M, Schürz S. 2020. Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations for a National Open Science Strategy in Austria, OANA, 36p."},"publisher":"OANA","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"date_created":"2020-10-23T09:29:45Z","file_size":2298363,"relation":"main_file","access_level":"open_access","checksum":"8eba912bb4b20b4f82f8010f2110461a","content_type":"application/pdf","file_name":"2020_OANA_Mayer.pdf","file_id":"8696","success":1,"creator":"dernst","date_updated":"2020-10-23T09:29:45Z"}],"author":[{"full_name":"Mayer, Katja","first_name":"Katja","last_name":"Mayer"},{"last_name":"Rieck","full_name":"Rieck, Katharina","first_name":"Katharina"},{"full_name":"Reichmann, Stefan","first_name":"Stefan","last_name":"Reichmann"},{"full_name":"Danowski, Patrick","first_name":"Patrick","orcid":"0000-0002-6026-4409","last_name":"Danowski","id":"2EBD1598-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Graschopf, Anton","first_name":"Anton","last_name":"Graschopf"},{"last_name":"König","first_name":"Thomas","full_name":"König, Thomas"},{"first_name":"Peter","full_name":"Kraker, Peter","last_name":"Kraker"},{"first_name":"Patrick","full_name":"Lehner, Patrick","last_name":"Lehner"},{"full_name":"Reckling, Falk","first_name":"Falk","last_name":"Reckling"},{"first_name":"Tony","full_name":"Ross-Hellauer, Tony","last_name":"Ross-Hellauer"},{"full_name":"Spichtinger, Daniel","first_name":"Daniel","last_name":"Spichtinger"},{"last_name":"Tzatzanis","full_name":"Tzatzanis, Michalis","first_name":"Michalis"},{"first_name":"Stefanie","full_name":"Schürz, Stefanie","last_name":"Schürz"}],"_id":"8695","language":[{"iso":"ger"}],"doi":"10.5281/ZENODO.4109242","oa_version":"Published Version","day":"21","publication_status":"published","oa":1,"file_date_updated":"2020-10-23T09:29:45Z","has_accepted_license":"1","month":"10","article_processing_charge":"No","date_updated":"2020-10-23T09:34:40Z"},{"day":"14","language":[{"iso":"ger"}],"_id":"8706","intvolume":"        73","file":[{"file_size":960317,"date_created":"2020-10-27T16:27:25Z","relation":"main_file","checksum":"37443c34d91d5bdbeb38c78b14792537","content_type":"application/pdf","access_level":"open_access","file_name":"2020_VOEB_Danowski.pdf","success":1,"file_id":"8714","creator":"kschuh","date_updated":"2020-10-27T16:27:25Z"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"corr_author":"1","date_created":"2020-10-25T23:01:19Z","year":"2020","article_processing_charge":"No","issue":"2","scopus_import":"1","publication_identifier":{"eissn":["1022-2588"]},"date_published":"2020-07-14T00:00:00Z","title":"„Recommendation“ for the further procedure for open access monitoring. Deliverable of the AT2OA subproject TP1-B","status":"public","oa_version":"Published Version","doi":"10.31263/voebm.v73i2.3941","author":[{"id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","last_name":"Danowski","orcid":"0000-0002-6026-4409","full_name":"Danowski, Patrick","first_name":"Patrick"},{"last_name":"Ferus","full_name":"Ferus, Andreas","first_name":"Andreas"},{"full_name":"Hikl, Anna-Laetitia","first_name":"Anna-Laetitia","last_name":"Hikl"},{"last_name":"McNeill","full_name":"McNeill, Gerda","first_name":"Gerda"},{"last_name":"Miniberger","first_name":"Clemens","full_name":"Miniberger, Clemens"},{"first_name":"Steve","full_name":"Reding, Steve","last_name":"Reding"},{"full_name":"Zarka, Tobias","first_name":"Tobias","last_name":"Zarka"},{"first_name":"Michael","full_name":"Zojer, Michael","last_name":"Zojer"}],"publisher":"Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare","citation":{"ama":"Danowski P, Ferus A, Hikl A-L, et al. „Recommendation“ for the further procedure for open access monitoring. Deliverable of the AT2OA subproject TP1-B. <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>. 2020;73(2):278-284. doi:<a href=\"https://doi.org/10.31263/voebm.v73i2.3941\">10.31263/voebm.v73i2.3941</a>","ista":"Danowski P, Ferus A, Hikl A-L, McNeill G, Miniberger C, Reding S, Zarka T, Zojer M. 2020. „Recommendation“ for the further procedure for open access monitoring. Deliverable of the AT2OA subproject TP1-B. Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 73(2), 278–284.","apa":"Danowski, P., Ferus, A., Hikl, A.-L., McNeill, G., Miniberger, C., Reding, S., … Zojer, M. (2020). „Recommendation“ for the further procedure for open access monitoring. Deliverable of the AT2OA subproject TP1-B. <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. <a href=\"https://doi.org/10.31263/voebm.v73i2.3941\">https://doi.org/10.31263/voebm.v73i2.3941</a>","ieee":"P. Danowski <i>et al.</i>, “„Recommendation“ for the further procedure for open access monitoring. Deliverable of the AT2OA subproject TP1-B,” <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>, vol. 73, no. 2. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, pp. 278–284, 2020.","chicago":"Danowski, Patrick, Andreas Ferus, Anna-Laetitia Hikl, Gerda McNeill, Clemens Miniberger, Steve Reding, Tobias Zarka, and Michael Zojer. “„Recommendation“ for the further procedure for open access monitoring. Deliverable of the AT2OA subproject TP1-B.” <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2020. <a href=\"https://doi.org/10.31263/voebm.v73i2.3941\">https://doi.org/10.31263/voebm.v73i2.3941</a>.","mla":"Danowski, Patrick, et al. “„Recommendation“ for the further procedure for open access monitoring. Deliverable of the AT2OA subproject TP1-B.” <i>Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare</i>, vol. 73, no. 2, Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2020, pp. 278–84, doi:<a href=\"https://doi.org/10.31263/voebm.v73i2.3941\">10.31263/voebm.v73i2.3941</a>.","short":"P. Danowski, A. Ferus, A.-L. Hikl, G. McNeill, C. Miniberger, S. Reding, T. Zarka, M. Zojer, Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare 73 (2020) 278–284."},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","ddc":["020"],"department":[{"_id":"E-Lib"}],"abstract":[{"lang":"eng","text":"As part of the Austrian Transition to Open Access (AT2OA) project, subproject TP1-B is working on designing a monitoring solution for the output of Open Access publications in Austria. This report on a potential Open Access monitoring approach in Austria is one of the results of these efforts and can serve as a basis for discussion on an international level."},{"text":"Als Teil des Hochschulraumstrukturmittel-Projekts Austrian Transition to Open Access (AT2OA) befasst sich das Teilprojekt TP1-B mit der Konzeption einer Monitoring-Lösung für den Open Access-Publikationsoutput in Österreich. Der nun vorliegende Bericht zu einem potentiellen Open Access-Monitoring in Österreich ist eines der Ergebnisse dieser Bemühungen und kann als Grundlage einer Diskussion auf internationaler Ebene dienen.","lang":"ger"}],"type":"journal_article","article_type":"original","page":"278-284","quality_controlled":"1","month":"07","publication":"Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare","date_updated":"2026-04-03T09:25:27Z","volume":73,"file_date_updated":"2020-10-27T16:27:25Z","oa":1,"publication_status":"published","has_accepted_license":"1"},{"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"file":[{"creator":"dernst","file_id":"8745","success":1,"date_updated":"2020-11-09T07:56:24Z","file_name":"2020_NatureComm_Schulte.pdf","access_level":"open_access","content_type":"application/pdf","checksum":"b2688f0347e69e6629bba582077278c5","file_size":1670898,"date_created":"2020-11-09T07:56:24Z","relation":"main_file"}],"day":"04","keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"_id":"8744","language":[{"iso":"eng"}],"intvolume":"        11","year":"2020","date_created":"2020-11-09T07:49:36Z","isi":1,"article_processing_charge":"No","publication_identifier":{"issn":["2041-1723"]},"scopus_import":"1","status":"public","title":"Cysteine oxidation and disulfide formation in the ribosomal exit tunnel","date_published":"2020-11-04T00:00:00Z","article_number":"5569","external_id":{"pmid":["33149120"],"isi":["000592028600001"]},"author":[{"last_name":"Schulte","first_name":"Linda","full_name":"Schulte, Linda"},{"last_name":"Mao","full_name":"Mao, Jiafei","first_name":"Jiafei"},{"full_name":"Reitz, Julian","first_name":"Julian","last_name":"Reitz"},{"last_name":"Sreeramulu","first_name":"Sridhar","full_name":"Sreeramulu, Sridhar"},{"full_name":"Kudlinzki, Denis","first_name":"Denis","last_name":"Kudlinzki"},{"first_name":"Victor-Valentin","full_name":"Hodirnau, Victor-Valentin","last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3904-947X"},{"full_name":"Meier-Credo, Jakob","first_name":"Jakob","last_name":"Meier-Credo"},{"last_name":"Saxena","full_name":"Saxena, Krishna","first_name":"Krishna"},{"last_name":"Buhr","first_name":"Florian","full_name":"Buhr, Florian"},{"first_name":"Julian D.","full_name":"Langer, Julian D.","last_name":"Langer"},{"first_name":"Martin","full_name":"Blackledge, Martin","last_name":"Blackledge"},{"first_name":"Achilleas S.","full_name":"Frangakis, Achilleas S.","last_name":"Frangakis"},{"last_name":"Glaubitz","full_name":"Glaubitz, Clemens","first_name":"Clemens"},{"last_name":"Schwalbe","full_name":"Schwalbe, Harald","first_name":"Harald"}],"publisher":"Springer Nature","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Schulte, Linda, et al. “Cysteine Oxidation and Disulfide Formation in the Ribosomal Exit Tunnel.” <i>Nature Communications</i>, vol. 11, 5569, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-19372-x\">10.1038/s41467-020-19372-x</a>.","short":"L. Schulte, J. Mao, J. Reitz, S. Sreeramulu, D. Kudlinzki, V.-V. Hodirnau, J. Meier-Credo, K. Saxena, F. Buhr, J.D. Langer, M. Blackledge, A.S. Frangakis, C. Glaubitz, H. Schwalbe, Nature Communications 11 (2020).","chicago":"Schulte, Linda, Jiafei Mao, Julian Reitz, Sridhar Sreeramulu, Denis Kudlinzki, Victor-Valentin Hodirnau, Jakob Meier-Credo, et al. “Cysteine Oxidation and Disulfide Formation in the Ribosomal Exit Tunnel.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-19372-x\">https://doi.org/10.1038/s41467-020-19372-x</a>.","apa":"Schulte, L., Mao, J., Reitz, J., Sreeramulu, S., Kudlinzki, D., Hodirnau, V.-V., … Schwalbe, H. (2020). Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-19372-x\">https://doi.org/10.1038/s41467-020-19372-x</a>","ista":"Schulte L, Mao J, Reitz J, Sreeramulu S, Kudlinzki D, Hodirnau V-V, Meier-Credo J, Saxena K, Buhr F, Langer JD, Blackledge M, Frangakis AS, Glaubitz C, Schwalbe H. 2020. Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. Nature Communications. 11, 5569.","ieee":"L. Schulte <i>et al.</i>, “Cysteine oxidation and disulfide formation in the ribosomal exit tunnel,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","ama":"Schulte L, Mao J, Reitz J, et al. Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-19372-x\">10.1038/s41467-020-19372-x</a>"},"oa_version":"Published Version","doi":"10.1038/s41467-020-19372-x","pmid":1,"ddc":["570"],"department":[{"_id":"EM-Fac"}],"abstract":[{"text":"Understanding the conformational sampling of translation-arrested ribosome nascent chain complexes is key to understand co-translational folding. Up to now, coupling of cysteine oxidation, disulfide bond formation and structure formation in nascent chains has remained elusive. Here, we investigate the eye-lens protein γB-crystallin in the ribosomal exit tunnel. Using mass spectrometry, theoretical simulations, dynamic nuclear polarization-enhanced solid-state nuclear magnetic resonance and cryo-electron microscopy, we show that thiol groups of cysteine residues undergo S-glutathionylation and S-nitrosylation and form non-native disulfide bonds. Thus, covalent modification chemistry occurs already prior to nascent chain release as the ribosome exit tunnel provides sufficient space even for disulfide bond formation which can guide protein folding.","lang":"eng"}],"type":"journal_article","article_type":"original","publication":"Nature Communications","quality_controlled":"1","month":"11","volume":11,"date_updated":"2025-06-12T07:01:22Z","publication_status":"published","file_date_updated":"2020-11-09T07:56:24Z","oa":1,"acknowledgement":"We acknowledge help from Anja Seybert, Margot Frangakis, Diana Grewe, Mikhail Eltsov, Utz Ermel, and Shintaro Aibara. The work was supported by Deutsche Forschungsgemeinschaft in the CLiC graduate school. Work at the Center for Biomolecular Magnetic Resonance (BMRZ) is supported by the German state of Hesse. The work at BMRZ has been supported by the state of Hesse. L.S. has been supported by the DFG graduate college: CLiC.","has_accepted_license":"1"},{"month":"11","quality_controlled":"1","publication":"Nature Communications","date_updated":"2026-04-02T11:48:21Z","volume":11,"file_date_updated":"2020-11-23T13:29:49Z","oa":1,"publication_status":"published","has_accepted_license":"1","acknowledgement":"We thank Sebastian Helmer, Nicole Blount, Christine Mann, and Beate Jantz for technical assistance; Hellen Ishikawa-Ankerhold for help and advice; Michael Sixt for critical\r\ndiscussions. This study was supported by the DFG SFB 914 (S.M. [B02 and Z01], K.Sch.\r\n[B02], B.W. [A02 and Z03], C.A.R. [B03], C.S. [A10], J.P. [Gerok position]), the DFG\r\nSFB 1123 (S.M. [B06]), the DFG FOR 2033 (S.M. and F.G.), the German Center for\r\nCardiovascular Research (DZHK) (Clinician Scientist Program [L.N.], MHA 1.4VD\r\n[S.M.], Postdoc Start-up Grant, 81×3600213 [F.G.]), FP7 program (project 260309,\r\nPRESTIGE [S.M.]), FöFoLe project 1015/1009 (L.N.), FöFoLe project 947 (F.G.), the\r\nFriedrich-Baur-Stiftung project 41/16 (F.G.), and LMUexcellence NFF (F.G.). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no.\r\n833440) (S.M.). F.G. received funding from the European Union’s Horizon 2020 research\r\nand innovation program under the Marie Skłodowska-Curie grant agreement no.\r\n747687.","title":"Vascular surveillance by haptotactic blood platelets in inflammation and infection","date_published":"2020-11-13T00:00:00Z","article_number":"5778","external_id":{"isi":["000594648000014"],"pmid":["33188196"]},"status":"public","oa_version":"Published Version","doi":"10.1038/s41467-020-19515-0","pmid":1,"author":[{"first_name":"Leo","full_name":"Nicolai, Leo","last_name":"Nicolai"},{"last_name":"Schiefelbein","full_name":"Schiefelbein, Karin","first_name":"Karin"},{"first_name":"Silvia","full_name":"Lipsky, Silvia","last_name":"Lipsky"},{"last_name":"Leunig","first_name":"Alexander","full_name":"Leunig, Alexander"},{"full_name":"Hoffknecht, Marie","first_name":"Marie","last_name":"Hoffknecht"},{"last_name":"Pekayvaz","first_name":"Kami","full_name":"Pekayvaz, Kami"},{"last_name":"Raude","full_name":"Raude, Ben","first_name":"Ben"},{"last_name":"Marx","full_name":"Marx, Charlotte","first_name":"Charlotte"},{"first_name":"Andreas","full_name":"Ehrlich, Andreas","last_name":"Ehrlich"},{"last_name":"Pircher","full_name":"Pircher, Joachim","first_name":"Joachim"},{"first_name":"Zhe","full_name":"Zhang, Zhe","last_name":"Zhang"},{"full_name":"Saleh, Inas","first_name":"Inas","last_name":"Saleh"},{"full_name":"Marel, Anna-Kristina","first_name":"Anna-Kristina","last_name":"Marel"},{"first_name":"Achim","full_name":"Löf, Achim","last_name":"Löf"},{"first_name":"Tobias","full_name":"Petzold, Tobias","last_name":"Petzold"},{"first_name":"Michael","full_name":"Lorenz, Michael","last_name":"Lorenz"},{"last_name":"Stark","first_name":"Konstantin","full_name":"Stark, Konstantin"},{"full_name":"Pick, Robert","first_name":"Robert","last_name":"Pick"},{"last_name":"Rosenberger","full_name":"Rosenberger, Gerhild","first_name":"Gerhild"},{"first_name":"Ludwig","full_name":"Weckbach, Ludwig","last_name":"Weckbach"},{"last_name":"Uhl","first_name":"Bernd","full_name":"Uhl, Bernd"},{"last_name":"Xia","full_name":"Xia, Sheng","first_name":"Sheng"},{"last_name":"Reichel","first_name":"Christoph Andreas","full_name":"Reichel, Christoph Andreas"},{"last_name":"Walzog","first_name":"Barbara","full_name":"Walzog, Barbara"},{"last_name":"Schulz","first_name":"Christian","full_name":"Schulz, Christian"},{"full_name":"Zheden, Vanessa","first_name":"Vanessa","orcid":"0000-0002-9438-4783","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","last_name":"Zheden"},{"last_name":"Bender","first_name":"Markus","full_name":"Bender, Markus"},{"last_name":"Li","first_name":"Rong","full_name":"Li, Rong"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"},{"orcid":"0000-0001-6120-3723","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","full_name":"Gärtner, Florian R"}],"citation":{"short":"L. Nicolai, K. Schiefelbein, S. Lipsky, A. Leunig, M. Hoffknecht, K. Pekayvaz, B. Raude, C. Marx, A. Ehrlich, J. Pircher, Z. Zhang, I. Saleh, A.-K. Marel, A. Löf, T. Petzold, M. Lorenz, K. Stark, R. Pick, G. Rosenberger, L. Weckbach, B. Uhl, S. Xia, C.A. Reichel, B. Walzog, C. Schulz, V. Zheden, M. Bender, R. Li, S. Massberg, F.R. Gärtner, Nature Communications 11 (2020).","mla":"Nicolai, Leo, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>, vol. 11, 5778, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>.","chicago":"Nicolai, Leo, Karin Schiefelbein, Silvia Lipsky, Alexander Leunig, Marie Hoffknecht, Kami Pekayvaz, Ben Raude, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>.","apa":"Nicolai, L., Schiefelbein, K., Lipsky, S., Leunig, A., Hoffknecht, M., Pekayvaz, K., … Gärtner, F. R. (2020). Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>","ista":"Nicolai L, Schiefelbein K, Lipsky S, Leunig A, Hoffknecht M, Pekayvaz K, Raude B, Marx C, Ehrlich A, Pircher J, Zhang Z, Saleh I, Marel A-K, Löf A, Petzold T, Lorenz M, Stark K, Pick R, Rosenberger G, Weckbach L, Uhl B, Xia S, Reichel CA, Walzog B, Schulz C, Zheden V, Bender M, Li R, Massberg S, Gärtner FR. 2020. Vascular surveillance by haptotactic blood platelets in inflammation and infection. Nature Communications. 11, 5778.","ieee":"L. Nicolai <i>et al.</i>, “Vascular surveillance by haptotactic blood platelets in inflammation and infection,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","ama":"Nicolai L, Schiefelbein K, Lipsky S, et al. Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>"},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publisher":"Springer Nature","ddc":["570"],"department":[{"_id":"MiSi"},{"_id":"EM-Fac"}],"abstract":[{"text":"Breakdown of vascular barriers is a major complication of inflammatory diseases. Anucleate platelets form blood-clots during thrombosis, but also play a crucial role in inflammation. While spatio-temporal dynamics of clot formation are well characterized, the cell-biological mechanisms of platelet recruitment to inflammatory micro-environments remain incompletely understood. Here we identify Arp2/3-dependent lamellipodia formation as a prominent morphological feature of immune-responsive platelets. Platelets use lamellipodia to scan for fibrin(ogen) deposited on the inflamed vasculature and to directionally spread, to polarize and to govern haptotactic migration along gradients of the adhesive ligand. Platelet-specific abrogation of Arp2/3 interferes with haptotactic repositioning of platelets to microlesions, thus impairing vascular sealing and provoking inflammatory microbleeding. During infection, haptotaxis promotes capture of bacteria and prevents hematogenic dissemination, rendering platelets gate-keepers of the inflamed microvasculature. Consequently, these findings identify haptotaxis as a key effector function of immune-responsive platelets.","lang":"eng"}],"type":"journal_article","article_type":"original","project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020"}],"article_processing_charge":"No","ec_funded":1,"scopus_import":"1","publication_identifier":{"eissn":["2041-1723"]},"day":"13","language":[{"iso":"eng"}],"_id":"8787","intvolume":"        11","file":[{"date_updated":"2020-11-23T13:29:49Z","file_id":"8798","creator":"dernst","success":1,"file_name":"2020_NatureComm_Nicolai.pdf","access_level":"open_access","checksum":"485b7b6cf30198ba0ce126491a28f125","content_type":"application/pdf","relation":"main_file","date_created":"2020-11-23T13:29:49Z","file_size":7035340}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"related_material":{"link":[{"url":"https://doi.org/10.1038/s41467-022-31310-7","relation":"erratum"}]},"corr_author":"1","date_created":"2020-11-22T23:01:23Z","year":"2020","isi":1},{"isi":1,"date_created":"2020-12-23T08:25:45Z","year":"2020","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"EM-Fac"}],"file":[{"success":1,"file_id":"8975","creator":"dernst","date_updated":"2020-12-28T08:16:10Z","file_name":"2020_NatureComm_Faessler.pdf","content_type":"application/pdf","checksum":"55d43ea0061cc4027ba45e966e1db8cc","access_level":"open_access","date_created":"2020-12-28T08:16:10Z","file_size":3958727,"relation":"main_file"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"corr_author":"1","related_material":{"link":[{"url":"https://ist.ac.at/en/news/cutting-edge-technology-reveals-structures-within-cells/","relation":"press_release","description":"News on IST Homepage"}]},"day":"22","keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"language":[{"iso":"eng"}],"_id":"8971","intvolume":"        11","publication_identifier":{"issn":["2041-1723"]},"scopus_import":"1","article_processing_charge":"No","project":[{"grant_number":"P33367","_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","name":"Structure and isoform diversity of the Arp2/3 complex"},{"name":"Protein structure and function in filopodia across scales","call_identifier":"FWF","_id":"2674F658-B435-11E9-9278-68D0E5697425","grant_number":"M02495"}],"article_type":"original","department":[{"_id":"FlSc"},{"_id":"EM-Fac"}],"ddc":["570"],"type":"journal_article","abstract":[{"text":"The actin-related protein (Arp)2/3 complex nucleates branched actin filament networks pivotal for cell migration, endocytosis and pathogen infection. Its activation is tightly regulated and involves complex structural rearrangements and actin filament binding, which are yet to be understood. Here, we report a 9.0 Å resolution structure of the actin filament Arp2/3 complex branch junction in cells using cryo-electron tomography and subtomogram averaging. This allows us to generate an accurate model of the active Arp2/3 complex in the branch junction and its interaction with actin filaments. Notably, our model reveals a previously undescribed set of interactions of the Arp2/3 complex with the mother filament, significantly different to the previous branch junction model. Our structure also indicates a central role for the ArpC3 subunit in stabilizing the active conformation.","lang":"eng"}],"author":[{"orcid":"0000-0001-7149-769X","id":"404F5528-F248-11E8-B48F-1D18A9856A87","last_name":"Fäßler","first_name":"Florian","full_name":"Fäßler, Florian"},{"id":"38C393BE-F248-11E8-B48F-1D18A9856A87","last_name":"Dimchev","orcid":"0000-0001-8370-6161","full_name":"Dimchev, Georgi A","first_name":"Georgi A"},{"full_name":"Hodirnau, Victor-Valentin","first_name":"Victor-Valentin","orcid":"0000-0003-3904-947X","last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Wan","first_name":"William","full_name":"Wan, William"},{"full_name":"Schur, Florian KM","first_name":"Florian KM","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4790-8078"}],"publisher":"Springer Nature","citation":{"ieee":"F. Fäßler, G. A. Dimchev, V.-V. Hodirnau, W. Wan, and F. K. Schur, “Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","apa":"Fäßler, F., Dimchev, G. A., Hodirnau, V.-V., Wan, W., &#38; Schur, F. K. (2020). Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-20286-x\">https://doi.org/10.1038/s41467-020-20286-x</a>","ista":"Fäßler F, Dimchev GA, Hodirnau V-V, Wan W, Schur FK. 2020. Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. Nature Communications. 11, 6437.","ama":"Fäßler F, Dimchev GA, Hodirnau V-V, Wan W, Schur FK. Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-20286-x\">10.1038/s41467-020-20286-x</a>","short":"F. Fäßler, G.A. Dimchev, V.-V. Hodirnau, W. Wan, F.K. Schur, Nature Communications 11 (2020).","mla":"Fäßler, Florian, et al. “Cryo-Electron Tomography Structure of Arp2/3 Complex in Cells Reveals New Insights into the Branch Junction.” <i>Nature Communications</i>, vol. 11, 6437, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-20286-x\">10.1038/s41467-020-20286-x</a>.","chicago":"Fäßler, Florian, Georgi A Dimchev, Victor-Valentin Hodirnau, William Wan, and Florian KM Schur. “Cryo-Electron Tomography Structure of Arp2/3 Complex in Cells Reveals New Insights into the Branch Junction.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-20286-x\">https://doi.org/10.1038/s41467-020-20286-x</a>."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","doi":"10.1038/s41467-020-20286-x","status":"public","title":"Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction","article_number":"6437","date_published":"2020-12-22T00:00:00Z","external_id":{"isi":["000603078000003"]},"acknowledgement":"This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), the BioImaging Facility (BIF), and the Electron Microscopy Facility (EMF). We also thank Dimitry Tegunov (MPI for Biophysical Chemistry) for helpful discussions\r\nabout the M software, and Michael Sixt (IST Austria) and Klemens Rottner (Technical University Braunschweig, HZI Braunschweig) for critical reading of the manuscript. We also thank Gregory Voth (University of Chicago) for providing us the MD-derived branch junction model for comparison. The authors acknowledge support from IST Austria and from the Austrian Science Fund (FWF): M02495 to G.D. and Austrian Science Fund (FWF): P33367 to F.K.M.S. ","has_accepted_license":"1","publication_status":"published","file_date_updated":"2020-12-28T08:16:10Z","oa":1,"volume":11,"date_updated":"2025-04-15T07:52:12Z","publication":"Nature Communications","quality_controlled":"1","month":"12"}]