[{"publication":"Molecular Therapy","title":"Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response","article_type":"original","OA_place":"repository","publisher":"Elsevier","day":"04","_id":"20858","year":"2025","date_published":"2025-10-04T00:00:00Z","oa":1,"quality_controlled":"1","date_created":"2025-12-28T23:01:26Z","abstract":[{"text":"Targeted antigen delivery to immune cells, particularly dendritic cells, has emerged as a promising strategy to enhance therapeutic efficacy of vaccines, while minimizing adverse effects associated with conventional immunization. In this study, we use our previously described small glycomimetic molecule that is selectively recognized by the Langerhans cell (LC)-specific surface receptor Langerin and demonstrate specific delivery of protein antigens to these specialized dendritic cells. Our results show that Langerin-mediated antigen delivery significantly enhances the immune response in vivo, resulting in increased expansion and activation of antigen-specific T cells, compared to immunization with unmodified antigen. We demonstrate the feasibility of our LC-targeted platform for immune cell-specific immunization with protein antigen and underscore the potential of LCs as an access point for next-generation vaccines and immunotherapies.","lang":"eng"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.05.05.652195"}],"publication_identifier":{"eissn":["1525-0024"],"issn":["1525-0016"]},"acknowledgement":"This project was generously supported by Seedfinancing (grant no. P2282679) of the Austrian Bundesministerium für Digitalisierung und Wirtschaftsstandort and the Bundesministerium für Klimaschutz, Umwelt, Energie, Mobilität, Innovation, und Technologie, handled by the Austrian Wirtschaftsservice (aws), as well as by Life Science Call 2022 (grant no. FO999896442) of the Austrian Research Promotion Agency (FFG). We thank Mag. Michael Schunn from the PCF of the Institute of Science and Technology Austria for his continuous technical support.","OA_type":"green","month":"10","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","language":[{"iso":"eng"}],"citation":{"ama":"Rica R, Klein K, Johnson L, et al. Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response. <i>Molecular Therapy</i>. doi:<a href=\"https://doi.org/10.1016/j.ymthe.2025.10.008\">10.1016/j.ymthe.2025.10.008</a>","apa":"Rica, R., Klein, K., Johnson, L., Carta, G., Sarcevic, M., Langer, F., … Sparber, F. (n.d.). Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response. <i>Molecular Therapy</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ymthe.2025.10.008\">https://doi.org/10.1016/j.ymthe.2025.10.008</a>","chicago":"Rica, Ramona, Klara Klein, Litty Johnson, Gabriele Carta, Mirza Sarcevic, Freyja Langer, Christoph Rademacher, Robert Wawrzinek, Federica Quattrone, and Florian Sparber. “Langerhans Cell-Targeted Protein Delivery Enhances Antigen-Specific Cellular Immune Response.” <i>Molecular Therapy</i>. Elsevier, n.d. <a href=\"https://doi.org/10.1016/j.ymthe.2025.10.008\">https://doi.org/10.1016/j.ymthe.2025.10.008</a>.","ista":"Rica R, Klein K, Johnson L, Carta G, Sarcevic M, Langer F, Rademacher C, Wawrzinek R, Quattrone F, Sparber F. Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response. Molecular Therapy.","short":"R. Rica, K. Klein, L. Johnson, G. Carta, M. Sarcevic, F. Langer, C. Rademacher, R. Wawrzinek, F. Quattrone, F. Sparber, Molecular Therapy (n.d.).","ieee":"R. Rica <i>et al.</i>, “Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response,” <i>Molecular Therapy</i>. Elsevier.","mla":"Rica, Ramona, et al. “Langerhans Cell-Targeted Protein Delivery Enhances Antigen-Specific Cellular Immune Response.” <i>Molecular Therapy</i>, Elsevier, doi:<a href=\"https://doi.org/10.1016/j.ymthe.2025.10.008\">10.1016/j.ymthe.2025.10.008</a>."},"department":[{"_id":"PreCl"}],"date_updated":"2025-12-29T09:55:05Z","author":[{"last_name":"Rica","full_name":"Rica, Ramona","first_name":"Ramona"},{"first_name":"Klara","last_name":"Klein","full_name":"Klein, Klara"},{"full_name":"Johnson, Litty","last_name":"Johnson","first_name":"Litty"},{"first_name":"Gabriele","full_name":"Carta, Gabriele","last_name":"Carta"},{"first_name":"Mirza","last_name":"Sarcevic","full_name":"Sarcevic, Mirza"},{"last_name":"Langer","full_name":"Langer, Freyja","first_name":"Freyja","id":"3C1BE782-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Rademacher","full_name":"Rademacher, Christoph","first_name":"Christoph"},{"first_name":"Robert","last_name":"Wawrzinek","full_name":"Wawrzinek, Robert"},{"first_name":"Federica","full_name":"Quattrone, Federica","last_name":"Quattrone"},{"first_name":"Florian","last_name":"Sparber","full_name":"Sparber, Florian"}],"article_processing_charge":"No","type":"journal_article","oa_version":"Preprint","publication_status":"inpress","doi":"10.1016/j.ymthe.2025.10.008","status":"public"},{"_id":"20859","day":"04","publisher":"Elsevier","ddc":["570"],"pmid":1,"publication":"Developmental Cell","PlanS_conform":"1","OA_place":"publisher","article_type":"original","title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses","main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2025.10.006","open_access":"1"}],"abstract":[{"text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo.","lang":"eng"}],"date_created":"2025-12-28T23:01:27Z","quality_controlled":"1","date_published":"2025-11-04T00:00:00Z","year":"2025","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa":1,"scopus_import":"1","has_accepted_license":"1","citation":{"ieee":"I. Company-Garrido <i>et al.</i>, “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” <i>Developmental Cell</i>. Elsevier, 2025.","short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell (2025).","ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2025. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell.","mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>.","ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. 2025. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>","apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2025). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>","chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>."},"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"NanoFab"}],"OA_type":"hybrid","external_id":{"pmid":["41192429"]},"acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"11","doi":"10.1016/j.devcel.2025.10.006","publication_status":"epub_ahead","oa_version":"Published Version","type":"journal_article","status":"public","date_updated":"2025-12-29T09:23:58Z","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"article_processing_charge":"Yes (in subscription journal)","author":[{"first_name":"Iván","last_name":"Company-Garrido","full_name":"Company-Garrido, Iván"},{"first_name":"Alberto","last_name":"Zurita Carpio","full_name":"Zurita Carpio, Alberto"},{"last_name":"Colomer-Rosell","full_name":"Colomer-Rosell, Mariona","first_name":"Mariona"},{"first_name":"Bernard","full_name":"Ciraulo, Bernard","last_name":"Ciraulo"},{"first_name":"Ronja","last_name":"Molkenbur","full_name":"Molkenbur, Ronja"},{"first_name":"Peter","last_name":"Lanzerstorfer","full_name":"Lanzerstorfer, Peter"},{"full_name":"Pezzano, Fabio","last_name":"Pezzano","first_name":"Fabio"},{"last_name":"Agazzi","full_name":"Agazzi, Costanza","first_name":"Costanza"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert"},{"full_name":"Jain, Saumey","last_name":"Jain","first_name":"Saumey"},{"last_name":"Jacques","full_name":"Jacques, Jeroen M.","first_name":"Jeroen M."},{"first_name":"Valeria","full_name":"Venturini, Valeria","last_name":"Venturini"},{"first_name":"Christian","full_name":"Knapp, Christian","last_name":"Knapp"},{"last_name":"Xie","full_name":"Xie, Yufei","first_name":"Yufei"},{"full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609"},{"full_name":"Weghuber, Julian","last_name":"Weghuber","first_name":"Julian"},{"full_name":"Schaaf, Marcel","last_name":"Schaaf","first_name":"Marcel"},{"full_name":"Quidant, Romain","last_name":"Quidant","first_name":"Romain"},{"last_name":"Kiermaier","full_name":"Kiermaier, Eva","first_name":"Eva","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6165-5738"},{"first_name":"Jaime","last_name":"Ortega Arroyo","full_name":"Ortega Arroyo, Jaime"},{"full_name":"Ruprecht, Verena","last_name":"Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633","first_name":"Verena"},{"full_name":"Wieser, Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","first_name":"Stefan"}]},{"publication_status":"published","date_created":"2025-12-29T12:16:22Z","abstract":[{"lang":"eng","text":"RNA sequencing (RNA-seq) methodologies have evolved rapidly, offering powerful tools to study gene expression, transcriptome dynamics, and molecular mechanisms in various biological contexts. However, the complexity of these approaches poses challenges in data interpretation, sensitivity, and applicability. This chapter provides a comprehensive overview of RNA-seq methodologies, highlighting their advantages, limitations, and applications, particularly in cardiovascular research. Bulk RNA sequencing enables high-throughput gene expression profiling but lacks the resolution to capture cellular heterogeneity and spatial context. Direct RNA sequencing preserves native RNA modifications, offering insights into post-transcriptional regulation, though it remains technically challenging. Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) bridge these gaps by resolving transcriptomic complexity at the cellular level and within tissue architecture, providing crucial insights into disease mechanisms such as atherosclerosis. By summarizing the strengths and limitations of these methodologies, this chapter aims to guide researchers in selecting the most suitable transcriptomic approach for their studies, ultimately advancing precision medicine and biomarker discovery in cardiovascular disease."}],"doi":"10.1016/b978-0-443-33064-3.00016-5","quality_controlled":"1","type":"book_chapter","oa_version":"None","status":"public","date_published":"2025-10-24T00:00:00Z","date_updated":"2026-01-05T11:49:54Z","year":"2025","department":[{"_id":"Bio"}],"article_processing_charge":"No","author":[{"full_name":"Stopa, Victoria","last_name":"Stopa","first_name":"Victoria"},{"last_name":"Sopić","full_name":"Sopić, Miron","first_name":"Miron"},{"last_name":"Li","full_name":"Li, Guanliang","first_name":"Guanliang"},{"first_name":"Judith","last_name":"Sluimer","full_name":"Sluimer, Judith"},{"first_name":"José","last_name":"Basílio","full_name":"Basílio, José"},{"first_name":"Sander W.","last_name":"van der Laan","full_name":"van der Laan, Sander W."},{"first_name":"David P.","full_name":"Kreil, David P.","last_name":"Kreil"},{"full_name":"Devaux, Yvan","last_name":"Devaux","first_name":"Yvan"},{"first_name":"Bernhard","id":"e6cab3de-17f6-11ed-9210-c1e42e045e9d","last_name":"Hochreiter","full_name":"Hochreiter, Bernhard"}],"_id":"20870","scopus_import":"1","publisher":"Elsevier","day":"24","citation":{"ieee":"V. Stopa <i>et al.</i>, “Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond,” in <i>Transcriptomics in Atherosclerosis</i>, Y. Devaux and M. Sopic, Eds. Elsevier, 2025, pp. 131–172.","short":"V. Stopa, M. Sopić, G. Li, J. Sluimer, J. Basílio, S.W. van der Laan, D.P. Kreil, Y. Devaux, B. Hochreiter, in:, Y. Devaux, M. Sopic (Eds.), Transcriptomics in Atherosclerosis, Elsevier, 2025, pp. 131–172.","ista":"Stopa V, Sopić M, Li G, Sluimer J, Basílio J, van der Laan SW, Kreil DP, Devaux Y, Hochreiter B. 2025.Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In: Transcriptomics in Atherosclerosis. , 131–172.","mla":"Stopa, Victoria, et al. “Essentials of Transcriptomic Methods: Navigating through RNA Sequencing and Beyond.” <i>Transcriptomics in Atherosclerosis</i>, edited by Yvan Devaux and Miron Sopic, Elsevier, 2025, pp. 131–72, doi:<a href=\"https://doi.org/10.1016/b978-0-443-33064-3.00016-5\">10.1016/b978-0-443-33064-3.00016-5</a>.","ama":"Stopa V, Sopić M, Li G, et al. Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In: Devaux Y, Sopic M, eds. <i>Transcriptomics in Atherosclerosis</i>. Elsevier; 2025:131-172. doi:<a href=\"https://doi.org/10.1016/b978-0-443-33064-3.00016-5\">10.1016/b978-0-443-33064-3.00016-5</a>","apa":"Stopa, V., Sopić, M., Li, G., Sluimer, J., Basílio, J., van der Laan, S. W., … Hochreiter, B. (2025). Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In Y. Devaux &#38; M. Sopic (Eds.), <i>Transcriptomics in Atherosclerosis</i> (pp. 131–172). Elsevier. <a href=\"https://doi.org/10.1016/b978-0-443-33064-3.00016-5\">https://doi.org/10.1016/b978-0-443-33064-3.00016-5</a>","chicago":"Stopa, Victoria, Miron Sopić, Guanliang Li, Judith Sluimer, José Basílio, Sander W. van der Laan, David P. Kreil, Yvan Devaux, and Bernhard Hochreiter. “Essentials of Transcriptomic Methods: Navigating through RNA Sequencing and Beyond.” In <i>Transcriptomics in Atherosclerosis</i>, edited by Yvan Devaux and Miron Sopic, 131–72. Elsevier, 2025. <a href=\"https://doi.org/10.1016/b978-0-443-33064-3.00016-5\">https://doi.org/10.1016/b978-0-443-33064-3.00016-5</a>."},"editor":[{"first_name":"Yvan","last_name":"Devaux","full_name":"Devaux, Yvan"},{"last_name":"Sopic","full_name":"Sopic, Miron","first_name":"Miron"}],"language":[{"iso":"eng"}],"publication":"Transcriptomics in Atherosclerosis","page":"131-172","OA_type":"closed access","publication_identifier":{"isbn":["9780443330643"]},"month":"10","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond"},{"status":"public","doi":"10.1101/2025.05.20.655037","publication_status":"draft","oa_version":"Preprint","type":"preprint","author":[{"last_name":"Dunajova","full_name":"Dunajova, Zuzana","id":"4B39F286-F248-11E8-B48F-1D18A9856A87","first_name":"Zuzana"},{"last_name":"Tasciyan","full_name":"Tasciyan, Saren","first_name":"Saren","orcid":"0000-0003-1671-393X","id":"4323B49C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Juraj","id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1","last_name":"Majek","full_name":"Majek, Juraj"},{"first_name":"Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","full_name":"Merrin, Jack"},{"last_name":"Sahai","full_name":"Sahai, Erik","first_name":"Erik"},{"full_name":"Sixt, Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","first_name":"Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"No","date_updated":"2026-03-18T14:11:35Z","department":[{"_id":"GradSch"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"AnSa"}],"has_accepted_license":"1","citation":{"short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B. Hannezo, (n.d.).","ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening.” bioRxiv.","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","mla":"Dunajova, Zuzana, et al. <i>Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening</i>. bioRxiv, doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K., &#38; Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. bioRxiv. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>","chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” bioRxiv, n.d. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>."},"language":[{"iso":"eng"}],"project":[{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793"},{"name":"Motile active matter models of migrating cells and chiral filaments","_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","grant_number":"26360"}],"corr_author":"1","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","month":"09","acknowledgement":"European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian Academy of Sciences, 26360","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"21423"},{"id":"21439","status":"public","relation":"research_data"}]},"main_file_link":[{"url":"https://doi.org/10.1101/2025.05.20.655037","open_access":"1"}],"date_created":"2026-03-11T08:40:06Z","abstract":[{"text":"While tumor malignancy has been extensively studied under the prism of genetic and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal interactions with the microenvironment. This raises the hitherto untested possibility that heterogeneity of the untransformed tumor stroma can actively fuel malignant progression. As biological heterogeneity is inherently difficult to control, we adopted a reductionist approach and let tumor cells invade micro-engineered environments harboring obstacles with precision-controlled geometry. We find that not only the presence of obstacles, but more surprisingly their spatial disorder, causes a drastic shift from a collective to a single-cell mode of invasion – comparable in strength to cadherin loss. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell detachments result both from local geometrical constraints and a global integration of spatial disorder over time. We show that different types of microenvironments map onto different universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang (KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with quenched disorder (KPZq). Our findings highlight generic physical principles for how the mode of cancer cell invasion depends on environmental heterogeneity, with potential implications to understand tumor evolution in vivo.","lang":"eng"}],"tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"oa":1,"date_published":"2025-09-25T00:00:00Z","year":"2025","ddc":["539","570"],"_id":"21427","day":"25","publisher":"bioRxiv","OA_place":"repository","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening"},{"quality_controlled":"1","abstract":[{"text":"Super-resolution microscopy often entails long acquisition times of minutes to hours. Since drifts during the acquisition adversely affect data quality, active sample stabilization is commonly used for some of these techniques to reach their full potential. Although drifts in the lateral plane can often be corrected after acquisition, this is not always possible or may come with drawbacks. Therefore, it is appealing to stabilize sample position in three dimensions (3D) during acquisition. Various schemes for active sample stabilization have been demonstrated previously, with some reaching sub-nanometer stability in 3D. Here, we present a scheme for active drift correction that delivers the nanometer-scale 3D stability demanded by state-of-the-art super-resolution techniques and is straightforward to implement compared to previous schemes capable of reaching this level of stabilization precision. Using a refined algorithm that can handle various types of reference structure, without sparse signal peaks being mandatory, we stabilized sample position to ∼1 nm in 3D using objective lenses both with high and low numerical aperture. Our implementation requires only the addition of a simple widefield imaging path and we provide an open-source control software with graphical user interface to facilitate easy adoption of the module. Finally, we demonstrate how this has the potential to enhance data collection for diffraction-limited and super-resolution imaging techniques using single-molecule localization microscopy and cryo-confocal imaging as showcases.","lang":"eng"}],"intvolume":"         5","date_created":"2025-06-08T22:01:22Z","year":"2025","issue":"2","date_published":"2025-06-11T00:00:00Z","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"publisher":"Elsevier","day":"11","_id":"19795","ddc":["570"],"publication":"Biophysical Reports","ec_funded":1,"title":"Image-based 3D active sample stabilization on the nanometer scale for optical microscopy","article_type":"original","OA_place":"publisher","volume":5,"type":"journal_article","oa_version":"Published Version","publication_status":"published","doi":"10.1016/j.bpr.2025.100211","status":"public","department":[{"_id":"JoDa"},{"_id":"GradSch"},{"_id":"FlSc"},{"_id":"EM-Fac"}],"date_updated":"2026-04-07T11:48:07Z","article_processing_charge":"Yes","author":[{"full_name":"Vorlaufer, Jakob","last_name":"Vorlaufer","first_name":"Jakob","id":"937696FA-C996-11E9-8C7C-CF13E6697425","orcid":"0009-0000-7590-3501"},{"last_name":"Semenov","full_name":"Semenov, Nikolai","first_name":"Nikolai","id":"e64d39c7-72ef-11ef-b75a-ee3046860d1b"},{"first_name":"Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87","full_name":"Kreuzinger, Caroline","last_name":"Kreuzinger"},{"first_name":"Manjunath","id":"305ab18b-dc7d-11ea-9b2f-b58195228ea2","orcid":"0000-0003-2311-2112","full_name":"Javoor, Manjunath","last_name":"Javoor"},{"first_name":"Bettina","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9561-1239","last_name":"Zens","full_name":"Zens, Bettina"},{"first_name":"Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","last_name":"Agudelo Duenas","full_name":"Agudelo Duenas, Nathalie"},{"last_name":"Tavakoli","full_name":"Tavakoli, Mojtaba","first_name":"Mojtaba","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7667-6854"},{"id":"EE8452B8-C26A-11E9-B157-E80CE6697425","first_name":"Marek","last_name":"Suplata","full_name":"Suplata, Marek"},{"last_name":"Jahr","full_name":"Jahr, Wiebke","first_name":"Wiebke","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0201-2315"},{"full_name":"Lyudchik, Julia","last_name":"Lyudchik","id":"46E28B80-F248-11E8-B48F-1D18A9856A87","first_name":"Julia"},{"last_name":"Wartak","full_name":"Wartak, Andreas","id":"60aaa06c-3de5-11eb-9e53-baa88e955dcb","first_name":"Andreas"},{"full_name":"Schur, Florian Km","last_name":"Schur","orcid":"0000-0003-4790-8078","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian Km"},{"first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8559-3973","full_name":"Danzl, Johann G","last_name":"Danzl"}],"corr_author":"1","file_date_updated":"2025-06-10T07:24:46Z","project":[{"_id":"62909c6f-2b32-11ec-9570-e1476aab5308","name":"CryoMinflux-guided in-situ molecular census and structure determination","grant_number":"CZI01"},{"name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5","grant_number":"26137"},{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program","call_identifier":"H2020","grant_number":"665385"},{"grant_number":"W1232-B24","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets","call_identifier":"FWF"},{"grant_number":"LT00057","name":"High-speed 3D-nanoscopy to study the role of adhesion during 3D cell migration","_id":"2668BFA0-B435-11E9-9278-68D0E5697425"}],"scopus_import":"1","language":[{"iso":"eng"}],"citation":{"short":"J. Vorlaufer, N. Semenov, C. Kreuzinger, M. Javoor, B. Zens, N. Agudelo Duenas, M. Tavakoli, M. Suplata, W. Jahr, J. Lyudchik, A. Wartak, F.K. Schur, J.G. Danzl, Biophysical Reports 5 (2025).","ista":"Vorlaufer J, Semenov N, Kreuzinger C, Javoor M, Zens B, Agudelo Duenas N, Tavakoli M, Suplata M, Jahr W, Lyudchik J, Wartak A, Schur FK, Danzl JG. 2025. Image-based 3D active sample stabilization on the nanometer scale for optical microscopy. Biophysical Reports. 5(2), 100211.","ieee":"J. Vorlaufer <i>et al.</i>, “Image-based 3D active sample stabilization on the nanometer scale for optical microscopy,” <i>Biophysical Reports</i>, vol. 5, no. 2. Elsevier, 2025.","mla":"Vorlaufer, Jakob, et al. “Image-Based 3D Active Sample Stabilization on the Nanometer Scale for Optical Microscopy.” <i>Biophysical Reports</i>, vol. 5, no. 2, 100211, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.bpr.2025.100211\">10.1016/j.bpr.2025.100211</a>.","apa":"Vorlaufer, J., Semenov, N., Kreuzinger, C., Javoor, M., Zens, B., Agudelo Duenas, N., … Danzl, J. G. (2025). Image-based 3D active sample stabilization on the nanometer scale for optical microscopy. <i>Biophysical Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.bpr.2025.100211\">https://doi.org/10.1016/j.bpr.2025.100211</a>","chicago":"Vorlaufer, Jakob, Nikolai Semenov, Caroline Kreuzinger, Manjunath Javoor, Bettina Zens, Nathalie Agudelo Duenas, Mojtaba Tavakoli, et al. “Image-Based 3D Active Sample Stabilization on the Nanometer Scale for Optical Microscopy.” <i>Biophysical Reports</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.bpr.2025.100211\">https://doi.org/10.1016/j.bpr.2025.100211</a>.","ama":"Vorlaufer J, Semenov N, Kreuzinger C, et al. Image-based 3D active sample stabilization on the nanometer scale for optical microscopy. <i>Biophysical Reports</i>. 2025;5(2). doi:<a href=\"https://doi.org/10.1016/j.bpr.2025.100211\">10.1016/j.bpr.2025.100211</a>"},"has_accepted_license":"1","article_number":"100211","publication_identifier":{"eissn":["2667-0747"]},"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"20206"}]},"file":[{"file_name":"2025_BiophysicalReports_Vorlaufer.pdf","date_updated":"2025-06-10T07:24:46Z","relation":"main_file","checksum":"4018c833f25a3ad3b57e3577fed70334","file_size":7238179,"file_id":"19802","success":1,"access_level":"open_access","date_created":"2025-06-10T07:24:46Z","content_type":"application/pdf","creator":"dernst"}],"acknowledgement":"We acknowledge expert support by ISTA’s scientific service units, including the Miba Machine Shop, the Electron Microscopy Facility, and the Lab Support Facility. This work has been made possible in part by CZI grant DAF2021-234754 and grant DOI: https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (funder DOI: https://doi.org/10.13039/100014989) (F.K.M.S. and J.G.D.). We further gratefully acknowledge funding by the following sources: Austrian Science Fund (FWF) grant DK W1232 (M.R.T. and J.G.D.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Marie Skłodowska-Curie Actions Fellowship GA no. 665385 under the EU Horizon 2020 program (J.L.); ISTA postdoctoral fellowship IST fellow (A.W.); and Human Frontier Science Program postdoctoral fellowship LT000557/2018 (W.J.).","OA_type":"gold","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"DOAJ_listed":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"06"},{"project":[{"grant_number":"771402","call_identifier":"H2020","name":"Epidemics in ant societies on a chip","_id":"2649B4DE-B435-11E9-9278-68D0E5697425"}],"file_date_updated":"2025-12-15T13:30:33Z","corr_author":"1","scopus_import":"1","language":[{"iso":"eng"}],"has_accepted_license":"1","article_number":"10511","citation":{"mla":"Dawson, Erika, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>, vol. 16, 10511, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>.","ieee":"E. Dawson <i>et al.</i>, “Altruistic disease signalling in ant colonies,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","ista":"Dawson E, Hönigsberger M, Kampleitner N, Grasse AV, Lindorfer L, Robb J, Beikzadeh F, Strahodinsky F, Leitner H, Rajendran H, Schmitt T, Cremer S. 2025. Altruistic disease signalling in ant colonies. Nature Communications. 16, 10511.","short":"E. Dawson, M. Hönigsberger, N. Kampleitner, A.V. Grasse, L. Lindorfer, J. Robb, F. Beikzadeh, F. Strahodinsky, H. Leitner, H. Rajendran, T. Schmitt, S. Cremer, Nature Communications 16 (2025).","ama":"Dawson E, Hönigsberger M, Kampleitner N, et al. Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-66175-z\">10.1038/s41467-025-66175-z</a>","chicago":"Dawson, Erika, Michaela Hönigsberger, Niklas Kampleitner, Anna V Grasse, Lukas Lindorfer, Jennifer Robb, Farnaz Beikzadeh, et al. “Altruistic Disease Signalling in Ant Colonies.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>.","apa":"Dawson, E., Hönigsberger, M., Kampleitner, N., Grasse, A. V., Lindorfer, L., Robb, J., … Cremer, S. (2025). Altruistic disease signalling in ant colonies. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-66175-z\">https://doi.org/10.1038/s41467-025-66175-z</a>"},"publication_identifier":{"eissn":["2041-1723"]},"OA_type":"gold","external_id":{"pmid":["41330896"]},"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"MassSpec"}],"related_material":{"record":[{"id":"20471","relation":"research_data","status":"public"}],"link":[{"url":"https://ista.ac.at/en/news/ants-signal-deadly-infection/","description":"News on ISTA website","relation":"press_release"}]},"file":[{"creator":"dernst","content_type":"application/pdf","file_id":"20826","date_created":"2025-12-15T13:30:33Z","success":1,"access_level":"open_access","checksum":"06244623bb7611c636652ecbc4787889","relation":"main_file","file_size":805323,"file_name":"2025_NatureComm_Dawson.pdf","date_updated":"2025-12-15T13:30:33Z"}],"acknowledgement":"We thank Joergen Eilenberg and Nicolai V. Meyling for the fungal strain, and the ISTA Social Immunity team, Jonghyun Park and Yuko Ulrich for ant collection. We also thank the Social Immunity team, in particular David Moreno Martínez, Tanvi Madaan, Wilfrid Jean Louis and Jessica Kirchner, for experimental and molecular support, as well as Friedrich Fochler for technical support with the chemical analysis, and the ISTA Lab Support Facility, including the mass spectrometry unit, for general and chemical laboratory support. We further thank Marco Ribezzi for advice on 13C calculations and Ernst Pittenauer for discussion of the chemical data, Chris Pull and Michael Sixt for project discussion, and the Social Immunity team for comments on the manuscript. The study was funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation Programme (No. 771402; EPIDEMICSonCHIP) to SC.","DOAJ_listed":"1","month":"12","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","oa_version":"Published Version","type":"journal_article","doi":"10.1038/s41467-025-66175-z","publication_status":"published","status":"public","department":[{"_id":"SyCr"},{"_id":"LifeSc"}],"date_updated":"2026-04-28T12:57:04Z","author":[{"full_name":"Dawson, Erika","last_name":"Dawson","first_name":"Erika","id":"31B4E2D0-F248-11E8-B48F-1D18A9856A87"},{"id":"953894f3-25bd-11ec-8556-f70a9d38ef60","first_name":"Michaela","full_name":"Hönigsberger, Michaela","last_name":"Hönigsberger"},{"first_name":"Niklas","id":"2AC57FAC-F248-11E8-B48F-1D18A9856A87","last_name":"Kampleitner","full_name":"Kampleitner, Niklas"},{"first_name":"Anna V","id":"406F989C-F248-11E8-B48F-1D18A9856A87","last_name":"Grasse","full_name":"Grasse, Anna V"},{"first_name":"Lukas","id":"85f0e6d3-06b3-11ec-8982-8c5049fa4455","last_name":"Lindorfer","full_name":"Lindorfer, Lukas"},{"id":"7bc2734a-e2c6-11ea-9824-a2ed5f0662a8","first_name":"Jennifer","last_name":"Robb","full_name":"Robb, Jennifer"},{"last_name":"Beikzadeh Abbasi","full_name":"Beikzadeh Abbasi, Farnaz","first_name":"Farnaz","id":"0344bfb9-3feb-11ee-87e9-c27edc800bcd"},{"id":"979E35EE-C996-11E9-8C7C-CF13E6697425","first_name":"Florian","full_name":"Strahodinsky, Florian","last_name":"Strahodinsky"},{"last_name":"Leitner","full_name":"Leitner, Hanna","first_name":"Hanna","id":"8fc5c6f6-5903-11ec-abad-c83f046253e7"},{"full_name":"Rajendran, Harikrishnan","last_name":"Rajendran","id":"876b6b34-8ff4-11ec-97c9-8d95a7aae416","first_name":"Harikrishnan"},{"full_name":"Schmitt, Thomas","last_name":"Schmitt","first_name":"Thomas"},{"id":"2F64EC8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2193-3868","first_name":"Sylvia","last_name":"Cremer","full_name":"Cremer, Sylvia"}],"article_processing_charge":"Yes","day":"01","publisher":"Springer Nature","_id":"18892","pmid":1,"ddc":["570"],"publication":"Nature Communications","PlanS_conform":"1","article_type":"original","title":"Altruistic disease signalling in ant colonies","ec_funded":1,"OA_place":"publisher","volume":16,"quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.02.27.582277"}],"date_created":"2025-01-27T11:28:05Z","abstract":[{"text":"Sick individuals often conceal their disease status to group members, thereby preventing social exclusion or aggression. Here we show by behavioural, chemical, immunological and infection load analyses that sick ant pupae instead actively emit a chemical signal that in itself is sufficient to trigger their own destruction by colony members. In our experiments, this altruistic disease-signalling was performed only by worker but not queen pupae. The lack of signalling by queen pupae did not constitute cheating behaviour, but reflected their superior immune capabilities. Worker pupae suffered from extensive pathogen replication whereas queen pupae were able to restrain their infection. Our data suggest the evolution of a finely-tuned signalling system in which it is not the induction of an individual’s immune response, but rather its failure to overcome the infection, that triggers pupal signalling for sacrifice. This demonstrates a balanced interplay between individual and social immunity that efficiently achieves whole-colony health.","lang":"eng"}],"intvolume":"        16","year":"2025","date_published":"2025-12-01T00:00:00Z","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"}},{"abstract":[{"text":"Oxygen redox chemistry is central to life1 and many human-made technologies, such as in energy storage2,3,4. The large energy gain from oxygen redox reactions is often connected with the occurrence of harmful reactive oxygen species3,5,6. Key species are superoxide and the highly reactive singlet oxygen3,4,5,6,7, which may evolve from superoxide. However, the factors determining the formation of singlet oxygen, rather than the relatively unreactive triplet oxygen, are unknown. Here we report that the release of triplet or singlet oxygen is governed by individual Marcus normal and inverted region behaviour. We found that as the driving force for the reaction increases, the initially dominant evolution of triplet oxygen slows down, and singlet oxygen evolution becomes predominant with higher maximum kinetics. This behaviour also applies to the widely observed superoxide disproportionation, in which one superoxide is oxidized by another, in both non-aqueous and aqueous systems, with Lewis and Brønsted acidity controlling the driving forces. Singlet oxygen yields governed by these conditions are relevant, for example, in batteries or cellular organelles in which superoxide forms. Our findings suggest ways to understand and control spin states and kinetics in oxygen redox chemistry, with implications for fields, including life sciences, pure chemistry and energy storage.","lang":"eng"}],"date_created":"2024-08-29T10:40:23Z","intvolume":"       646","quality_controlled":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa":1,"date_published":"2025-10-16T00:00:00Z","issue":"8085","year":"2025","ddc":["540"],"pmid":1,"_id":"17468","day":"16","publisher":"Springer Nature","OA_place":"publisher","volume":646,"article_type":"original","title":"Marcus kinetics control singlet and triplet oxygen evolving from superoxide","publication":"Nature","PlanS_conform":"1","isi":1,"status":"public","doi":"10.1038/s41586-025-09587-7","publication_status":"published","oa_version":"Published Version","type":"journal_article","article_processing_charge":"Yes (via OA deal)","author":[{"full_name":"Mondal, Soumyadip","last_name":"Mondal","first_name":"Soumyadip","id":"d25d21ef-dc8d-11ea-abe3-ec4576307f48"},{"last_name":"Nguyen","full_name":"Nguyen, Huyen T.K.","first_name":"Huyen T.K."},{"full_name":"Hauschild, Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","first_name":"Robert"},{"last_name":"Freunberger","full_name":"Freunberger, Stefan Alexander","first_name":"Stefan Alexander","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425","orcid":"0000-0003-2902-5319"}],"date_updated":"2026-04-28T13:18:33Z","department":[{"_id":"StFr"},{"_id":"Bio"}],"has_accepted_license":"1","citation":{"ieee":"S. Mondal, H. T. K. Nguyen, R. Hauschild, and S. A. Freunberger, “Marcus kinetics control singlet and triplet oxygen evolving from superoxide,” <i>Nature</i>, vol. 646, no. 8085. Springer Nature, pp. 601–605, 2025.","ista":"Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. 2025. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. Nature. 646(8085), 601–605.","short":"S. Mondal, H.T.K. Nguyen, R. Hauschild, S.A. Freunberger, Nature 646 (2025) 601–605.","mla":"Mondal, Soumyadip, et al. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” <i>Nature</i>, vol. 646, no. 8085, Springer Nature, 2025, pp. 601–605, doi:<a href=\"https://doi.org/10.1038/s41586-025-09587-7\">10.1038/s41586-025-09587-7</a>.","apa":"Mondal, S., Nguyen, H. T. K., Hauschild, R., &#38; Freunberger, S. A. (2025). Marcus kinetics control singlet and triplet oxygen evolving from superoxide. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-09587-7\">https://doi.org/10.1038/s41586-025-09587-7</a>","chicago":"Mondal, Soumyadip, Huyen T.K. Nguyen, Robert Hauschild, and Stefan Alexander Freunberger. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-09587-7\">https://doi.org/10.1038/s41586-025-09587-7</a>.","ama":"Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. <i>Nature</i>. 2025;646(8085):601–605. doi:<a href=\"https://doi.org/10.1038/s41586-025-09587-7\">10.1038/s41586-025-09587-7</a>"},"language":[{"iso":"eng"}],"scopus_import":"1","file_date_updated":"2025-10-20T10:26:13Z","project":[{"name":"Singlet oxygen in non-aqueous oxygen redox chemistry","_id":"8df062be-16d5-11f0-9cad-f559b6612c7e","grant_number":"P37169"},{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"}],"corr_author":"1","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","month":"10","external_id":{"isi":["001586378900001"],"pmid":["41044415"]},"OA_type":"hybrid","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"ScienComp"}],"file":[{"file_name":"2025_Nature_Mondal.pdf","date_updated":"2025-10-20T10:26:13Z","relation":"main_file","checksum":"b507ddd23df0388aa65d04dc9b00fe3d","file_size":3809247,"file_id":"20500","success":1,"access_level":"open_access","date_created":"2025-10-20T10:26:13Z","content_type":"application/pdf","creator":"dernst"}],"acknowledgement":"S.A.F. thanks the Institute of Science and Technology Austria (ISTA) for the support. The Scientific Service Units of ISTA supported this research through resources provided by the Imaging and Optics Facility, the Lab Support Facility, the Miba Machine Shop and Scientific Computing. This research was partly funded by the Austrian Science Fund (FWF) (10.55776/P37169 and 10.55776/COE5). For open access purposes, the author has applied for a CC BY public copyright licence to any author-accepted manuscript version arising from this submission. R.H. acknowledges funding through CZI grant DAF2020-225401 (10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (10.13039/100014989). H.T.K.N. acknowledges funding by the European Commission Erasmus Mundus Joint Masters programme. We thank M. Sixt and M. Chinon for the discussions about O-redox in life and R. Jethwa for proofreading. Open access funding was provided by ISTA.","related_material":{"link":[{"url":"https://ista.ac.at/en/news/taming-the-bad-oxygen/","description":"News on ISTA website","relation":"press_release"}]},"page":"601–605","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]}},{"OA_place":"publisher","volume":26,"article_type":"letter_note","title":"Migrating immune cells globally coordinate protrusive forces","PlanS_conform":"1","publication":"Nature Immunology","ddc":["570"],"pmid":1,"_id":"20082","publisher":"Springer Nature","day":"01","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa":1,"date_published":"2025-08-01T00:00:00Z","year":"2025","abstract":[{"lang":"eng","text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence."}],"intvolume":"        26","date_created":"2025-07-27T22:01:26Z","quality_controlled":"1","month":"08","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","file":[{"file_name":"2025_NatureImmunology_ReisRodrigues.pdf","date_updated":"2025-07-31T08:00:33Z","checksum":"0c725123dca7797c682609bff2c4c5ac","relation":"main_file","file_size":13514646,"file_id":"20096","date_created":"2025-07-31T08:00:33Z","access_level":"open_access","success":1,"creator":"dernst","content_type":"application/pdf"}],"page":"1258–1266","acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","related_material":{"link":[{"description":"News on ISTA website","url":"https://ista.ac.at/en/news/bench-pressing-cells/","relation":"press_release"}],"record":[{"id":"20149","relation":"dissertation_contains","status":"public"}]},"OA_type":"hybrid","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"external_id":{"pmid":["40664976"],"isi":["001529134300001"]},"publication_identifier":{"eissn":["1529-2916"],"issn":["1529-2908"]},"citation":{"chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>.","apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>","ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. 2025;26:1258–1266. doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>","mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>.","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.","ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","ieee":"P. Dos Reis Rodrigues <i>et al.</i>, “Migrating immune cells globally coordinate protrusive forces,” <i>Nature Immunology</i>, vol. 26. Springer Nature, pp. 1258–1266, 2025."},"has_accepted_license":"1","language":[{"iso":"eng"}],"scopus_import":"1","corr_author":"1","project":[{"_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","grant_number":"101071793"},{"grant_number":"944-2020","_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8","name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration"}],"file_date_updated":"2025-07-31T08:00:33Z","article_processing_charge":"Yes (via OA deal)","author":[{"full_name":"Dos Reis Rodrigues, Patricia","last_name":"Dos Reis Rodrigues","orcid":"0000-0003-1681-508X","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","first_name":"Patricia"},{"id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","orcid":"0000-0001-6406-524X","first_name":"Mario","full_name":"Avellaneda Sarrió, Mario","last_name":"Avellaneda Sarrió"},{"first_name":"Nikola","orcid":"0000-0002-8518-5926","id":"3795523E-F248-11E8-B48F-1D18A9856A87","last_name":"Canigova","full_name":"Canigova, Nikola"},{"last_name":"Gärtner","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R"},{"full_name":"Vaahtomeri, Kari","last_name":"Vaahtomeri","first_name":"Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7829-3518"},{"last_name":"Riedl","full_name":"Riedl, Michael","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4844-6311","first_name":"Michael"},{"full_name":"De Vries, Ingrid","last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid"},{"orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","last_name":"Merrin","full_name":"Merrin, Jack"},{"full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Fukui, Yoshinori","last_name":"Fukui","first_name":"Yoshinori"},{"first_name":"Alba","id":"40F05888-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1009-9652","full_name":"Juanes Garcia, Alba","last_name":"Juanes Garcia"},{"full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K"}],"date_updated":"2026-04-28T13:26:50Z","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"isi":1,"status":"public","publication_status":"published","doi":"10.1038/s41590-025-02211-w","type":"journal_article","oa_version":"Published Version"},{"language":[{"iso":"eng"}],"has_accepted_license":"1","citation":{"chicago":"Tavakoli, Mojtaba, Julia Lyudchik, Michał Januszewski, Vitali Vistunou, Nathalie Agudelo Duenas, Jakob Vorlaufer, Christoph M Sommer, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-08985-1\">https://doi.org/10.1038/s41586-025-08985-1</a>.","apa":"Tavakoli, M., Lyudchik, J., Januszewski, M., Vistunou, V., Agudelo Duenas, N., Vorlaufer, J., … Danzl, J. G. (2025). Light-microscopy-based connectomic reconstruction of mammalian brain tissue. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-08985-1\">https://doi.org/10.1038/s41586-025-08985-1</a>","ama":"Tavakoli M, Lyudchik J, Januszewski M, et al. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. <i>Nature</i>. 2025;642:398-410. doi:<a href=\"https://doi.org/10.1038/s41586-025-08985-1\">10.1038/s41586-025-08985-1</a>","mla":"Tavakoli, Mojtaba, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” <i>Nature</i>, vol. 642, Springer Nature, 2025, pp. 398–410, doi:<a href=\"https://doi.org/10.1038/s41586-025-08985-1\">10.1038/s41586-025-08985-1</a>.","ista":"Tavakoli M, Lyudchik J, Januszewski M, Vistunou V, Agudelo Duenas N, Vorlaufer J, Sommer CM, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, Jain V, Danzl JG. 2025. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. Nature. 642, 398–410.","short":"M. Tavakoli, J. Lyudchik, M. Januszewski, V. Vistunou, N. Agudelo Duenas, J. Vorlaufer, C.M. Sommer, C. Kreuzinger, B. Oliveira, A. Cenameri, G. Novarino, V. Jain, J.G. Danzl, Nature 642 (2025) 398–410.","ieee":"M. Tavakoli <i>et al.</i>, “Light-microscopy-based connectomic reconstruction of mammalian brain tissue,” <i>Nature</i>, vol. 642. Springer Nature, pp. 398–410, 2025."},"project":[{"grant_number":"26137","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5","name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy"},{"name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"665385"},{"_id":"34ba8964-11ca-11ed-8bc3-e15864e7e9a6","name":"Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders","grant_number":"101044865"},{"grant_number":"W1232-B24","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets","call_identifier":"FWF"}],"file_date_updated":"2025-07-03T06:55:20Z","corr_author":"1","scopus_import":"1","month":"06","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"OA_type":"hybrid","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"},{"_id":"PreCl"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"external_id":{"pmid":["40335689"],"isi":["001483477000001"]},"acknowledgement":"We thank S. Dorkenwald and P. Li for critical reading of the manuscript, S. Loomba for discussions and E. Miguel for support with data handling. We acknowledge support from ISTA’s scientific service units: Imaging and Optics, Lab Support, Scientific Computing, the preclinical facility, the Miba Machine Shop and the library. We acknowledge funding from the following sources: Austrian Science Fund (FWF) grant DK W1232 (J.G.D. and M.R.T.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); the European Union’s Horizon 2020 research and innovation programme and Marie Skłodowska-Curie Actions Fellowship 665385 (J.L.); and the European Union’s Horizon 2020 research and innovation programme and European Research Council (ERC) grant 101044865 ‘SecretAutism’ (G.N.).Open access funding provided by Institute of Science and Technology (IST Austria).","file":[{"success":1,"access_level":"open_access","date_created":"2025-07-03T06:55:20Z","file_id":"19959","content_type":"application/pdf","creator":"dernst","file_name":"2025_Nature_Tavakoli.pdf","date_updated":"2025-07-03T06:55:20Z","file_size":133201290,"relation":"main_file","checksum":"ebc99d7108e728f46db0a009292675ef"}],"related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/piecing-together-the-brain-puzzle/","description":"News on ISTA website"}],"record":[{"id":"18677","status":"public","relation":"earlier_version"},{"id":"18697","relation":"research_data","status":"public"}]},"page":"398-410","status":"public","isi":1,"oa_version":"Published Version","type":"journal_article","doi":"10.1038/s41586-025-08985-1","publication_status":"published","author":[{"first_name":"Mojtaba","orcid":"0000-0002-7667-6854","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","full_name":"Tavakoli, Mojtaba","last_name":"Tavakoli"},{"first_name":"Julia","id":"46E28B80-F248-11E8-B48F-1D18A9856A87","last_name":"Lyudchik","full_name":"Lyudchik, Julia"},{"first_name":"Michał","full_name":"Januszewski, Michał","last_name":"Januszewski"},{"full_name":"Vistunou, Vitali","last_name":"Vistunou","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","first_name":"Vitali"},{"full_name":"Agudelo Duenas, Nathalie","last_name":"Agudelo Duenas","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","first_name":"Nathalie"},{"last_name":"Vorlaufer","full_name":"Vorlaufer, Jakob","orcid":"0009-0000-7590-3501","id":"937696FA-C996-11E9-8C7C-CF13E6697425","first_name":"Jakob"},{"first_name":"Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","full_name":"Sommer, Christoph M"},{"id":"382077BA-F248-11E8-B48F-1D18A9856A87","first_name":"Caroline","last_name":"Kreuzinger","full_name":"Kreuzinger, Caroline"},{"first_name":"Bárbara","id":"3B03AA1A-F248-11E8-B48F-1D18A9856A87","full_name":"Oliveira, Bárbara","last_name":"Oliveira"},{"last_name":"Cenameri","full_name":"Cenameri, Alban","id":"9ac8f577-2357-11eb-997a-e566c5550886","first_name":"Alban"},{"first_name":"Gaia","orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","full_name":"Novarino, Gaia","last_name":"Novarino"},{"last_name":"Jain","full_name":"Jain, Viren","first_name":"Viren"},{"full_name":"Danzl, Johann G","last_name":"Danzl","first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8559-3973"}],"article_processing_charge":"Yes (via OA deal)","department":[{"_id":"JoDa"},{"_id":"GradSch"},{"_id":"Bio"},{"_id":"GaNo"}],"date_updated":"2026-04-28T13:33:34Z","pmid":1,"ddc":["570"],"day":"12","publisher":"Springer Nature","_id":"19704","article_type":"original","title":"Light-microscopy-based connectomic reconstruction of mammalian brain tissue","ec_funded":1,"OA_place":"publisher","volume":642,"publication":"Nature","PlanS_conform":"1","quality_controlled":"1","intvolume":"       642","date_created":"2025-05-18T22:02:51Z","abstract":[{"text":"The information-processing capability of the brain’s cellular network depends on the physical wiring pattern between neurons and their molecular and functional characteristics. Mapping neurons and resolving their individual synaptic connections can be achieved by volumetric imaging at nanoscale resolution1,2 with dense cellular labelling. Light microscopy is uniquely positioned to visualize specific molecules, but dense, synapse-level circuit reconstruction by light microscopy has been out of reach, owing to limitations in resolution, contrast and volumetric imaging capability. Here we describe light-microscopy-based connectomics (LICONN). We integrated specifically engineered hydrogel embedding and expansion with comprehensive deep-learning-based segmentation and analysis of connectivity, thereby directly incorporating molecular information into synapse-level reconstructions of brain tissue. LICONN will allow synapse-level phenotyping of brain tissue in biological experiments in a readily adoptable manner.","lang":"eng"}],"oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"year":"2025","date_published":"2025-06-12T00:00:00Z"},{"quality_controlled":"1","abstract":[{"lang":"eng","text":"Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of nonfunctional “promoter leakiness,” merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the noncanonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g., control of costly-to-induce multidrug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multidrug resistance is crucial for effective public health measures."}],"intvolume":"       122","date_created":"2025-04-27T22:02:13Z","year":"2025","issue":"15","date_published":"2025-04-15T00:00:00Z","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"publisher":"National Academy of Sciences","day":"15","_id":"19626","pmid":1,"ddc":["570"],"publication":"Proceedings of the National Academy of Sciences","article_type":"original","title":"Pulsatile basal gene expression as a fitness determinant in bacteria","OA_place":"publisher","volume":122,"type":"journal_article","oa_version":"Published Version","publication_status":"published","doi":"10.1073/pnas.2413709122","status":"public","isi":1,"department":[{"_id":"CaGu"},{"_id":"Bio"},{"_id":"FyKo"},{"_id":"GaTk"}],"date_updated":"2026-04-28T13:35:46Z","article_processing_charge":"Yes (in subscription journal)","author":[{"first_name":"Kirti","orcid":"0000-0002-3809-0449","id":"330F0278-F248-11E8-B48F-1D18A9856A87","last_name":"Jain","full_name":"Jain, Kirti"},{"first_name":"Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"full_name":"Bochkareva, Olga","last_name":"Bochkareva","first_name":"Olga","orcid":"0000-0003-1006-6639","id":"C4558D3C-6102-11E9-A62E-F418E6697425"},{"last_name":"Römhild","full_name":"Römhild, Roderich","id":"68E56E44-62B0-11EA-B963-444F3DDC885E","orcid":"0000-0001-9480-5261","first_name":"Roderich"},{"last_name":"Tkačik","full_name":"Tkačik, Gašper","first_name":"Gašper","orcid":"0000-0002-6699-1455","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","first_name":"Calin C","last_name":"Guet","full_name":"Guet, Calin C"}],"corr_author":"1","file_date_updated":"2025-06-24T07:27:43Z","project":[{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"},{"_id":"bd6f94d1-d553-11ed-ba76-ae9f07250f74","name":"Non-canonical antibiotic interactions","grant_number":"E219"},{"_id":"34e076d6-11ca-11ed-8bc3-aec76c41a181","name":"Evolutionary analysis of gene regulation","grant_number":"I05127"}],"scopus_import":"1","language":[{"iso":"eng"}],"citation":{"chicago":"Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2413709122\">https://doi.org/10.1073/pnas.2413709122</a>.","apa":"Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., &#38; Guet, C. C. (2025). Pulsatile basal gene expression as a fitness determinant in bacteria. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2413709122\">https://doi.org/10.1073/pnas.2413709122</a>","ama":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Pulsatile basal gene expression as a fitness determinant in bacteria. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(15). doi:<a href=\"https://doi.org/10.1073/pnas.2413709122\">10.1073/pnas.2413709122</a>","mla":"Jain, Kirti, et al. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 15, e2413709122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2413709122\">10.1073/pnas.2413709122</a>.","short":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, Proceedings of the National Academy of Sciences 122 (2025).","ista":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Pulsatile basal gene expression as a fitness determinant in bacteria. Proceedings of the National Academy of Sciences. 122(15), e2413709122.","ieee":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Pulsatile basal gene expression as a fitness determinant in bacteria,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 15. National Academy of Sciences, 2025."},"article_number":"e2413709122","has_accepted_license":"1","publication_identifier":{"issn":["0027-8424"],"eissn":["1091-6490"]},"acknowledgement":"K.J. thanks B. Wu, I. Tomanek, K. Tomasek for detailed discussions on the manuscript, all other members from the Guet laboratory for valuable feedback, R. Chait, & Imaging and Optics Facility, Institute of Science and Technology Austria for helping with microscopy, Dr. Sudha Rao and Dr. Raja Mugasimangalam, Genotypic Technology India for allowing time off to address the revisions. K.J. acknowledges Institute of Science and Technology fellowship IC1006FELL02, R.H. was supported in part by Chan Zuckerberg Initiative and Donor Advised-Fund grant 2020-225401 (https://doi.org/10.37921/120055ratwvi), O.O.B. acknowledges Fonds Zur Förderung der Wissenschaftlichen Forschung (FWF) Grant ESP253-B, R.R. acknowledges FWF Grant 10.55776/ESP219, C.C.G. acknowledges FWF I5127-B.","file":[{"file_size":2949523,"relation":"main_file","checksum":"115a687f40009660eb4b38b4f6559d41","date_updated":"2025-06-24T07:27:43Z","file_name":"2025_PNAS_Jain.pdf","creator":"dernst","content_type":"application/pdf","success":1,"access_level":"open_access","date_created":"2025-06-24T07:27:43Z","file_id":"19888"}],"related_material":{"record":[{"relation":"research_data","status":"public","id":"19294"}],"link":[{"description":"News on ISTA website","url":"https://ista.ac.at/en/news/clockwork-just-for-antibiotic-resistance/","relation":"press_release"}]},"acknowledged_ssus":[{"_id":"Bio"}],"external_id":{"pmid":["40193613"],"isi":["001471235200001"]},"OA_type":"hybrid","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","month":"04"},{"status":"public","date_created":"2025-03-04T13:27:21Z","abstract":[{"lang":"eng","text":"Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of non-functional “promoter leakiness”, merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the non-canonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g. control of costly-to-induce multi-drug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multi-drug resistance is crucial for effective public health measures."}],"doi":"10.15479/AT:ISTA:19294","type":"research_data","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","author":[{"last_name":"Jain","full_name":"Jain, Kirti","orcid":"0000-0002-3809-0449","id":"330F0278-F248-11E8-B48F-1D18A9856A87","first_name":"Kirti"},{"first_name":"Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"last_name":"Bochkareva","full_name":"Bochkareva, Olga","id":"C4558D3C-6102-11E9-A62E-F418E6697425","orcid":"0000-0003-1006-6639","first_name":"Olga"},{"id":"68E56E44-62B0-11EA-B963-444F3DDC885E","orcid":"0000-0001-9480-5261","first_name":"Roderich","last_name":"Römhild","full_name":"Römhild, Roderich"},{"id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6699-1455","first_name":"Gašper","full_name":"Tkačik, Gašper","last_name":"Tkačik"},{"full_name":"Guet, Calin C","last_name":"Guet","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6220-2052","first_name":"Calin C"}],"oa":1,"date_published":"2025-03-04T00:00:00Z","date_updated":"2026-04-28T13:35:45Z","year":"2025","department":[{"_id":"CaGu"},{"_id":"Bio"},{"_id":"FyKo"},{"_id":"GaTk"}],"ddc":["570"],"citation":{"ista":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>.","ieee":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria.’” Institute of Science and Technology Austria, 2025.","short":"K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, (2025).","mla":"Jain, Kirti, et al. <i>Data for “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.”</i> Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:19294\">10.15479/AT:ISTA:19294</a>.","ama":"Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 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Data for “Pulsatile basal gene expression as a fitness determinant in bacteria.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">https://doi.org/10.15479/AT:ISTA:19294</a>","chicago":"Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Data for ‘Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.’” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT:ISTA:19294\">https://doi.org/10.15479/AT:ISTA:19294</a>."},"has_accepted_license":"1","_id":"19294","corr_author":"1","publisher":"Institute of Science and Technology Austria","day":"04","file_date_updated":"2025-03-05T07:39:38Z","month":"03","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_place":"repository","title":"Data for \"Pulsatile basal gene expression as a fitness determinant in bacteria\"","file":[{"file_name":"Data1.xlsx","date_updated":"2025-03-04T13:08:52Z","relation":"main_file","checksum":"11a5bab307a4e1e1598a1577d8a2fbb5","file_size":269054,"file_id":"19295","access_level":"open_access","success":1,"date_created":"2025-03-04T13:08:52Z","creator":"dernst","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet"},{"checksum":"3b057894322639f0c1e11fb2e84173e6","relation":"main_file","file_size":87143,"file_name":"Data2.xlsx","date_updated":"2025-03-04T13:08:52Z","creator":"dernst","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","file_id":"19296","date_created":"2025-03-04T13:08:52Z","success":1,"access_level":"open_access"},{"file_name":"Data3.xlsx","date_updated":"2025-03-04T13:08:52Z","checksum":"a551e1b79a138bb97ab96979aa475b3c","relation":"main_file","file_size":129101,"file_id":"19297","date_created":"2025-03-04T13:08:52Z","access_level":"open_access","success":1,"creator":"dernst","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet"},{"date_updated":"2025-03-04T13:08:52Z","file_name":"Data4.xlsx","relation":"main_file","checksum":"d6909c9bf111f859058082b1a2f970c4","file_size":86243,"file_id":"19298","access_level":"open_access","success":1,"date_created":"2025-03-04T13:08:52Z","creator":"dernst","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet"},{"creator":"dernst","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","access_level":"open_access","success":1,"date_created":"2025-03-04T13:08:52Z","file_id":"19299","file_size":26049,"relation":"main_file","checksum":"e5725a3a118a3f06846104906c8792c7","date_updated":"2025-03-04T13:08:52Z","file_name":"Data5.xlsx"},{"file_size":7327253,"checksum":"16763c127049f14bd587dc885677dce1","relation":"main_file","date_updated":"2025-03-04T13:08:52Z","file_name":"RawData_2_3.xlsx","creator":"dernst","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","date_created":"2025-03-04T13:08:52Z","access_level":"open_access","success":1,"file_id":"19300"},{"date_created":"2025-03-05T07:39:38Z","access_level":"open_access","success":1,"file_id":"19301","creator":"dernst","content_type":"text/plain","file_name":"Readme.txt","date_updated":"2025-03-05T07:39:38Z","file_size":606,"checksum":"2f3e1a368b4e3abc46bf37e02724f0f4","relation":"main_file"}],"related_material":{"record":[{"status":"public","relation":"used_in_publication","id":"19626"}]},"OA_type":"gold"},{"intvolume":"       638","abstract":[{"lang":"eng","text":"When two insulating, neutral materials are contacted and separated, they exchange electrical charge1. Experiments have long suggested that this ‘contact electrification’ is transitive, with different materials ordering into ‘triboelectric series’ based on the sign of charge acquired2. At the same time, the effect is plagued by unpredictability, preventing consensus on the mechanism and casting doubt on the rhyme and reason that series imply3. Here we expose an unanticipated connection between the unpredictability and order in contact electrification: nominally identical materials initially exchange charge randomly and intransitively, but—over repeated experiments—order into triboelectric series. We find that this evolution is driven by the act of contact itself—samples with more contacts in their history charge negatively to ones with fewer contacts. Capturing this ‘contact bias’ in a minimal model, we recreate both the initial randomness and ultimate order in numerical simulations and use it experimentally to force the appearance of a triboelectric series of our choosing. With a set of surface-sensitive techniques to search for the underlying alterations contact creates, we only find evidence of nanoscale morphological changes, pointing to a mechanism strongly coupled with mechanics. Our results highlight the centrality of contact history in contact electrification and suggest that focusing on the unpredictability that has long plagued the effect may hold the key to understanding it."}],"date_created":"2025-03-02T23:01:52Z","quality_controlled":"1","date_published":"2025-02-20T00:00:00Z","issue":"8051","year":"2025","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa":1,"_id":"19278","day":"20","publisher":"Springer Nature","ddc":["530"],"pmid":1,"publication":"Nature","OA_place":"publisher","volume":638,"title":"Spontaneous ordering of identical materials into a triboelectric series","article_type":"original","ec_funded":1,"doi":"10.1038/s41586-024-08530-6","publication_status":"published","oa_version":"Published Version","type":"journal_article","isi":1,"status":"public","date_updated":"2026-04-28T13:44:56Z","department":[{"_id":"ScWa"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"author":[{"full_name":"Sobarzo Ponce, Juan Carlos A","last_name":"Sobarzo Ponce","first_name":"Juan Carlos A","id":"4B807D68-AE37-11E9-AC72-31CAE5697425"},{"orcid":"0000-0003-0463-5794","id":"6313aec0-15b2-11ec-abd3-ed67d16139af","first_name":"Felix","full_name":"Pertl, Felix","last_name":"Pertl"},{"id":"302BADF6-85FC-11EA-9E3B-B9493DDC885E","orcid":"0000-0001-7597-043X","first_name":"Daniel","full_name":"Balazs, Daniel","last_name":"Balazs"},{"full_name":"Costanzo, Tommaso","last_name":"Costanzo","first_name":"Tommaso","orcid":"0000-0001-9732-3815","id":"D93824F4-D9BA-11E9-BB12-F207E6697425"},{"last_name":"Sauer","full_name":"Sauer, Markus","first_name":"Markus"},{"first_name":"Annette","last_name":"Foelske","full_name":"Foelske, Annette"},{"first_name":"Markus","full_name":"Ostermann, Markus","last_name":"Ostermann"},{"last_name":"Pichler","full_name":"Pichler, Christian M.","first_name":"Christian M."},{"first_name":"Yongkang","last_name":"Wang","full_name":"Wang, Yongkang"},{"full_name":"Nagata, Yuki","last_name":"Nagata","first_name":"Yuki"},{"last_name":"Bonn","full_name":"Bonn, Mischa","first_name":"Mischa"},{"last_name":"Waitukaitis","full_name":"Waitukaitis, Scott R","orcid":"0000-0002-2299-3176","id":"3A1FFC16-F248-11E8-B48F-1D18A9856A87","first_name":"Scott R"}],"article_processing_charge":"Yes (via OA deal)","scopus_import":"1","project":[{"grant_number":"949120","call_identifier":"H2020","_id":"0aa60e99-070f-11eb-9043-a6de6bdc3afa","name":"Tribocharge: a multi-scale approach to an enduring problem in physics"}],"file_date_updated":"2025-03-04T10:05:18Z","corr_author":"1","article_number":"664-669","has_accepted_license":"1","citation":{"ama":"Sobarzo Ponce JCA, Pertl F, Balazs D, et al. Spontaneous ordering of identical materials into a triboelectric series. <i>Nature</i>. 2025;638(8051). doi:<a href=\"https://doi.org/10.1038/s41586-024-08530-6\">10.1038/s41586-024-08530-6</a>","apa":"Sobarzo Ponce, J. C. A., Pertl, F., Balazs, D., Costanzo, T., Sauer, M., Foelske, A., … Waitukaitis, S. R. (2025). Spontaneous ordering of identical materials into a triboelectric series. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-024-08530-6\">https://doi.org/10.1038/s41586-024-08530-6</a>","chicago":"Sobarzo Ponce, Juan Carlos A, Felix Pertl, Daniel Balazs, Tommaso Costanzo, Markus Sauer, Annette Foelske, Markus Ostermann, et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-024-08530-6\">https://doi.org/10.1038/s41586-024-08530-6</a>.","ista":"Sobarzo Ponce JCA, Pertl F, Balazs D, Costanzo T, Sauer M, Foelske A, Ostermann M, Pichler CM, Wang Y, Nagata Y, Bonn M, Waitukaitis SR. 2025. Spontaneous ordering of identical materials into a triboelectric series. Nature. 638(8051), 664–669.","ieee":"J. C. A. Sobarzo Ponce <i>et al.</i>, “Spontaneous ordering of identical materials into a triboelectric series,” <i>Nature</i>, vol. 638, no. 8051. Springer Nature, 2025.","short":"J.C.A. Sobarzo Ponce, F. Pertl, D. Balazs, T. Costanzo, M. Sauer, A. Foelske, M. Ostermann, C.M. Pichler, Y. Wang, Y. Nagata, M. Bonn, S.R. Waitukaitis, Nature 638 (2025).","mla":"Sobarzo Ponce, Juan Carlos A., et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” <i>Nature</i>, vol. 638, no. 8051, 664–669, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41586-024-08530-6\">10.1038/s41586-024-08530-6</a>."},"language":[{"iso":"eng"}],"external_id":{"pmid":["39972227"],"isi":["001428076100015"]},"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"NanoFab"},{"_id":"ScienComp"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"OA_type":"hybrid","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"20203"}],"link":[{"url":"https://ista.ac.at/en/news/an-electrifying-turn-in-an-age-old-quest/","description":"News on ISTA website","relation":"press_release"}]},"file":[{"checksum":"fecf302274dd3218d3e7dd22f39a6c0c","relation":"main_file","file_size":3807415,"date_updated":"2025-03-04T10:05:18Z","file_name":"2025_Nature_Sobarzo.pdf","content_type":"application/pdf","creator":"dernst","file_id":"19289","date_created":"2025-03-04T10:05:18Z","access_level":"open_access","success":1}],"acknowledgement":"This project has received financing from the European Research Council grant agreement no. 949120 under the European Union’s Horizon 2020 research and innovation programme. The Analytical Instrumentation Center of the TU Wien acknowledges support by the FFG project ‘ELSA’ under grant no. 884672. C.M.P. and M.O. acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility, Electron Microscopy Facility and Lab Support Facility. We thank J. Garcia-Suarez and G. Anciaux for the suggestion to look into the roughness power spectral density. We thank I.-M. Strugaru for help with testing the device for Young’s modulus measurements. Open access funding provided by Institute of Science and Technology (IST Austria).","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"month":"02","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd"},{"author":[{"id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8703-1093","first_name":"Stefanie","full_name":"Rus, Stefanie","last_name":"Rus"},{"first_name":"David","orcid":"0000-0001-7205-2975","id":"e1e86031-6537-11eb-953a-f7ab92be508d","last_name":"Brückner","full_name":"Brückner, David"},{"last_name":"Minchington","full_name":"Minchington, Thomas","id":"7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f","first_name":"Thomas"},{"first_name":"Martina","id":"48A59534-F248-11E8-B48F-1D18A9856A87","last_name":"Greunz","full_name":"Greunz, Martina"},{"full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609"},{"first_name":"Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"full_name":"Kicheva, Anna","last_name":"Kicheva","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4509-4998","first_name":"Anna"}],"article_processing_charge":"Yes (via OA deal)","department":[{"_id":"AnKi"},{"_id":"EdHa"},{"_id":"NanoFab"}],"date_updated":"2026-05-13T22:31:09Z","status":"public","isi":1,"type":"journal_article","oa_version":"Published Version","publication_status":"published","doi":"10.1016/j.devcel.2024.10.024","month":"02","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["1534-5807"]},"page":"567-580","related_material":{"record":[{"id":"19763","status":"public","relation":"dissertation_contains"}]},"file":[{"relation":"main_file","checksum":"bb58db4a908a1f4aabe4004706154541","file_size":6994499,"file_name":"2025_DevelopmentalCell_Lehr.pdf","date_updated":"2025-04-16T10:54:07Z","creator":"dernst","content_type":"application/pdf","file_id":"19584","success":1,"access_level":"open_access","date_created":"2025-04-16T10:54:07Z"}],"acknowledgement":"We thank A. Miller and N. Papalopulu for reagents and J. Briscoe for comments on the manuscript. Work in the A.K. lab is supported by ISTA; the European Research Council under Horizon Europe, grant 101044579; and the Austrian Science Fund (FWF), grant https://doi.org/10.55776/F78. S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022).","OA_type":"hybrid","external_id":{"isi":["001434279000001"],"pmid":["39603235"]},"language":[{"iso":"eng"}],"citation":{"apa":"Rus, S., Brückner, D., Minchington, T., Greunz, M., Merrin, J., Hannezo, E. B., &#38; Kicheva, A. (2025). Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>","chicago":"Rus, Stefanie, David Brückner, Thomas Minchington, Martina Greunz, Jack Merrin, Edouard B Hannezo, and Anna Kicheva. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>.","ama":"Rus S, Brückner D, Minchington T, et al. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. 2025;60(4):567-580. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>","ista":"Rus S, Brückner D, Minchington T, Greunz M, Merrin J, Hannezo EB, Kicheva A. 2025. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. Developmental Cell. 60(4), 567–580.","ieee":"S. Rus <i>et al.</i>, “Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling,” <i>Developmental Cell</i>, vol. 60, no. 4. Elsevier, pp. 567–580, 2025.","short":"S. Rus, D. Brückner, T. Minchington, M. Greunz, J. Merrin, E.B. Hannezo, A. Kicheva, Developmental Cell 60 (2025) 567–580.","mla":"Rus, Stefanie, et al. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>, vol. 60, no. 4, Elsevier, 2025, pp. 567–80, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>."},"has_accepted_license":"1","corr_author":"1","file_date_updated":"2025-04-16T10:54:07Z","project":[{"grant_number":"101044579","_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","name":"Mechanisms of tissue size regulation in spinal cord development"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen control of growth and pattern in the spinal cord","_id":"059DF620-7A3F-11EA-A408-12923DDC885E","grant_number":"F7802"},{"grant_number":"SC19-011","name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube","_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A"}],"scopus_import":"1","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"year":"2025","issue":"4","date_published":"2025-02-24T00:00:00Z","quality_controlled":"1","date_created":"2025-01-09T11:25:47Z","intvolume":"        60","abstract":[{"lang":"eng","text":"Developing tissues interpret dynamic changes in morphogen activity to generate cell type diversity. To quantitatively study bone morphogenetic protein (BMP) signaling dynamics in the mouse neural tube, we developed an embryonic stem cell differentiation system tailored for growing tissues. Differentiating cells form striking self-organized patterns of dorsal neural tube cell types driven by sequential phases of BMP signaling that are observed both in vitro and in vivo. Data-driven biophysical modeling showed that these dynamics result from coupling fast negative feedback with slow positive regulation of signaling by the specification of an endogenous BMP source. Thus, in contrast to relays that propagate morphogen signaling in space, we identify a BMP signaling relay that operates in time. This mechanism allows for a rapid initial concentration-sensitive response that is robustly terminated, thereby regulating balanced sequential cell type generation. Our study provides an experimental and theoretical framework to understand how signaling dynamics are exploited in developing tissues."}],"title":"Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling","article_type":"original","volume":60,"OA_place":"publisher","publication":"Developmental Cell","pmid":1,"ddc":["570"],"publisher":"Elsevier","day":"24","_id":"18807"},{"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa":1,"date_published":"2024-08-29T00:00:00Z","year":"2024","intvolume":"        15","abstract":[{"lang":"eng","text":"The formation of new ribosomes is tightly coordinated with cell growth and proliferation. In eukaryotes, the correct assembly of all ribosomal proteins and RNAs follows an intricate scheme of maturation and rearrangement steps across three cellular compartments: the nucleolus, nucleoplasm, and cytoplasm. We demonstrate that usnic acid, a lichen secondary metabolite, inhibits the maturation of the large ribosomal subunit in yeast. We combine biochemical characterization of pre-ribosomal particles with a quantitative single-particle cryo-EM approach to monitor changes in nucleolar particle populations upon drug treatment. Usnic acid rapidly blocks the transition from nucleolar state B to C of Nsa1-associated pre-ribosomes, depleting key maturation factors such as Dbp10 and hindering pre-rRNA processing. This primary nucleolar block rapidly rebounds on earlier stages of the pathway which highlights the regulatory linkages between different steps. In summary, we provide an in-depth characterization of the effect of usnic acid on ribosome biogenesis, which may have implications for its reported anti-cancer activities."}],"date_created":"2024-09-08T22:01:10Z","quality_controlled":"1","OA_place":"publisher","volume":15,"title":"The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation","article_type":"original","publication":"Nature Communications","ddc":["570"],"pmid":1,"_id":"17885","publisher":"Springer Nature","day":"29","article_processing_charge":"Yes","author":[{"first_name":"Lisa","last_name":"Kofler","full_name":"Kofler, Lisa"},{"full_name":"Grundmann, Lorenz","last_name":"Grundmann","first_name":"Lorenz"},{"full_name":"Gerhalter, Magdalena","last_name":"Gerhalter","first_name":"Magdalena"},{"last_name":"Prattes","full_name":"Prattes, Michael","first_name":"Michael"},{"last_name":"Merl-Pham","full_name":"Merl-Pham, Juliane","first_name":"Juliane"},{"first_name":"Gertrude","full_name":"Zisser, Gertrude","last_name":"Zisser"},{"last_name":"Grishkovskaya","full_name":"Grishkovskaya, Irina","first_name":"Irina"},{"first_name":"Victor-Valentin","id":"3661B498-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3904-947X","full_name":"Hodirnau, Victor-Valentin","last_name":"Hodirnau"},{"full_name":"Vareka, Martin","last_name":"Vareka","first_name":"Martin"},{"first_name":"Rolf","last_name":"Breinbauer","full_name":"Breinbauer, Rolf"},{"last_name":"Hauck","full_name":"Hauck, Stefanie M.","first_name":"Stefanie M."},{"first_name":"David","last_name":"Haselbach","full_name":"Haselbach, David"},{"first_name":"Helmut","last_name":"Bergler","full_name":"Bergler, Helmut"}],"date_updated":"2025-09-08T09:13:01Z","department":[{"_id":"EM-Fac"}],"isi":1,"status":"public","publication_status":"published","doi":"10.1038/s41467-024-51754-3","type":"journal_article","oa_version":"Published Version","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"08","DOAJ_listed":"1","acknowledgement":"We thank Michael A. McAlear, Micheline Fromont-Racin, Philipp Milkereit, Arlen W. Johnson, Sabine Rospert, Ed Hurt, C. Yam, Günter Daum, Wolfgang Zachariae, Katrin Karbstein, Juan P. G. Ballesta, Mercedes Dosil, Miguel Remacha und Jesus de la Cruz for sharing strains or providing antibodies. We thank the members of the Bergler lab and the Haselbach lab for their helpful discussion. We thank Ellen Zhong for helpful discussions about the quantitative cryoDRGN analysis. This research was supported by the Scientific Service Units of IST Austria through resources provided by the Electron Microscopy Facility. This research was funded in whole, or in part, by the Austrian Science Foundation grants [https://doi.org/10.55776/P32977], [https://doi.org/10.55776/P29451] and [https://doi.org/10.55776/P32536] (to H.B.). Research at the IMP is generously supported by Boehringer Ingelheim and the Austrian Research Promotion Agency (Headquarter grant FFG-852936). For the purpose of open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.","file":[{"file_id":"17946","success":1,"access_level":"open_access","date_created":"2024-09-09T08:56:12Z","content_type":"application/pdf","creator":"dernst","date_updated":"2024-09-09T08:56:12Z","file_name":"2024_NatureComm_Kofler.pdf","relation":"main_file","checksum":"7c044538a47182c826d1b526c52958a2","file_size":3735024}],"acknowledged_ssus":[{"_id":"EM-Fac"}],"external_id":{"pmid":["39209816"],"isi":["001457895200001"]},"OA_type":"gold","publication_identifier":{"eissn":["2041-1723"]},"citation":{"ama":"Kofler L, Grundmann L, Gerhalter M, et al. The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation. <i>Nature Communications</i>. 2024;15. doi:<a href=\"https://doi.org/10.1038/s41467-024-51754-3\">10.1038/s41467-024-51754-3</a>","chicago":"Kofler, Lisa, Lorenz Grundmann, Magdalena Gerhalter, Michael Prattes, Juliane Merl-Pham, Gertrude Zisser, Irina Grishkovskaya, et al. “The Novel Ribosome Biogenesis Inhibitor Usnic Acid Blocks Nucleolar Pre-60S Maturation.” <i>Nature Communications</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41467-024-51754-3\">https://doi.org/10.1038/s41467-024-51754-3</a>.","apa":"Kofler, L., Grundmann, L., Gerhalter, M., Prattes, M., Merl-Pham, J., Zisser, G., … Bergler, H. (2024). The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-024-51754-3\">https://doi.org/10.1038/s41467-024-51754-3</a>","mla":"Kofler, Lisa, et al. “The Novel Ribosome Biogenesis Inhibitor Usnic Acid Blocks Nucleolar Pre-60S Maturation.” <i>Nature Communications</i>, vol. 15, 7511, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1038/s41467-024-51754-3\">10.1038/s41467-024-51754-3</a>.","ista":"Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau V-V, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. 2024. The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation. Nature Communications. 15, 7511.","ieee":"L. Kofler <i>et al.</i>, “The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation,” <i>Nature Communications</i>, vol. 15. Springer Nature, 2024.","short":"L. Kofler, L. Grundmann, M. Gerhalter, M. Prattes, J. Merl-Pham, G. Zisser, I. Grishkovskaya, V.-V. Hodirnau, M. Vareka, R. Breinbauer, S.M. Hauck, D. Haselbach, H. Bergler, Nature Communications 15 (2024)."},"has_accepted_license":"1","article_number":"7511","language":[{"iso":"eng"}],"scopus_import":"1","file_date_updated":"2024-09-09T08:56:12Z"},{"editor":[{"first_name":"Joachim H.R. ","full_name":"Lübke, Joachim H.R. ","last_name":"Lübke"},{"last_name":"Rollenhagen","full_name":"Rollenhagen, Astrid","first_name":"Astrid"}],"day":"27","publisher":"Springer Nature","_id":"18052","title":"Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning","ec_funded":1,"publication":"New Aspects in Analyzing the Synaptic Organization of the Brain","quality_controlled":"1","date_created":"2024-09-10T12:32:38Z","abstract":[{"lang":"eng","text":"Sodium dodecyl sulfate-digested freeze-fracture replica labeling (SDS-FRL) is an electron microscope (EM) sample preparation technique which allows for high-resolution visualization of membrane proteins with high sensitivity. However, image acquisition of specific replica profiles such as synapses in a large field of EM view needs a valid experience and a long time for manual searching. Here, we describe how to utilize deep learning for automatizing image acquisition of specific profiles of interest in replica samples. This protocol facilitates the labor-intensive collection of EM images, in particular for rare profiles. We provide instructions for using SerialEM image acquisition software in conjunction with object detection by our newly developed deep learning software DarEM, to automatically acquire tilt series of all synapses in a selected region. We then show how to perform a mostly automated analysis of gold particle labeling in the acquired images by utilizing Darea software."}],"alternative_title":["Neuromethods"],"year":"2024","date_published":"2024-08-27T00:00:00Z","language":[{"iso":"eng"}],"citation":{"ama":"Kleindienst D, Costanzo T, Shigemoto R. Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In: Lübke JHR, Rollenhagen A, eds. <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>. 1st ed. New York: Springer Nature; 2024:123-137. doi:<a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">10.1007/978-1-0716-4019-7_8</a>","apa":"Kleindienst, D., Costanzo, T., &#38; Shigemoto, R. (2024). Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In J. H. R. Lübke &#38; A. Rollenhagen (Eds.), <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i> (1st ed., pp. 123–137). New York: Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">https://doi.org/10.1007/978-1-0716-4019-7_8</a>","chicago":"Kleindienst, David, Tommaso Costanzo, and Ryuichi Shigemoto. “Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning.” In <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>, edited by Joachim H.R.  Lübke and Astrid Rollenhagen, 1st ed., 123–37. New York: Springer Nature, 2024. <a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">https://doi.org/10.1007/978-1-0716-4019-7_8</a>.","ieee":"D. Kleindienst, T. Costanzo, and R. Shigemoto, “Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning,” in <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>, 1st ed., J. H. R. Lübke and A. Rollenhagen, Eds. New York: Springer Nature, 2024, pp. 123–137.","ista":"Kleindienst D, Costanzo T, Shigemoto R. 2024.Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning. In: New Aspects in Analyzing the Synaptic Organization of the Brain. Neuromethods, , 123–137.","short":"D. Kleindienst, T. Costanzo, R. Shigemoto, in:, J.H.R. Lübke, A. Rollenhagen (Eds.), New Aspects in Analyzing the Synaptic Organization of the Brain, 1st ed., Springer Nature, New York, 2024, pp. 123–137.","mla":"Kleindienst, David, et al. “Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning.” <i>New Aspects in Analyzing the Synaptic Organization of the Brain</i>, edited by Joachim H.R.  Lübke and Astrid Rollenhagen, 1st ed., Springer Nature, 2024, pp. 123–37, doi:<a href=\"https://doi.org/10.1007/978-1-0716-4019-7_8\">10.1007/978-1-0716-4019-7_8</a>."},"project":[{"call_identifier":"H2020","_id":"25CA28EA-B435-11E9-9278-68D0E5697425","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour","grant_number":"694539"}],"corr_author":"1","scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"08","publication_identifier":{"issn":["0893-2336"],"eissn":["1940-6045"],"isbn":["9781071640180"],"eisbn":["9781071640197"]},"place":"New York","acknowledged_ssus":[{"_id":"EM-Fac"}],"page":"123-137","acknowledgement":"This research was supported by the European Research Council Advanced Grant 694539 to RS and by the Scientific Service Units of IST Austria through resources provided by the Electron Microscopy Facility.","status":"public","edition":"1","oa_version":"None","type":"book_chapter","doi":"10.1007/978-1-0716-4019-7_8","publication_status":"published","author":[{"first_name":"David","id":"42E121A4-F248-11E8-B48F-1D18A9856A87","last_name":"Kleindienst","full_name":"Kleindienst, David"},{"last_name":"Costanzo","full_name":"Costanzo, Tommaso","first_name":"Tommaso","id":"D93824F4-D9BA-11E9-BB12-F207E6697425","orcid":"0000-0001-9732-3815"},{"first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi"}],"article_processing_charge":"No","department":[{"_id":"EM-Fac"},{"_id":"RySh"}],"date_updated":"2025-04-14T07:27:15Z"},{"publisher":"American Chemical Society","day":"11","_id":"18168","pmid":1,"ddc":["540"],"publication":"Biomacromolecules","title":"Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein","article_type":"original","volume":25,"quality_controlled":"1","date_created":"2024-10-02T10:09:53Z","intvolume":"        25","abstract":[{"text":"Despite the considerable interest in the recombinant production of synthetic spider silk fibers that possess mechanical properties similar to those of native spider silks, such as the cost-effectiveness, tunability, and scalability realization, is still lacking. To address this long-standing challenge, we have constructed an artificial spider silk gene using Golden Gate assembly for the recombinant bacterial production of dragline-mimicking silk, incorporating all the essential components: the N-terminal domain, a 33-residue-long major-ampullate-spidroin-inspired segment repeated 16 times, and the C-terminal domain (N16C). This designed silk-like protein was successfully expressed in Escherichia coli, purified, and cast into films from formic acid. We produced uniformly 13C–15N-labeled N16C films and employed solid-state magic-angle spinning nuclear magnetic resonance (NMR) for characterization. Thus, we could demonstrate that our bioengineered silk-like protein self-assembles into a film where, when hydrated, the solvent-exposed layer of the rigid, β-nanocrystalline polyalanine core undergoes a transition to an α-helical structure, gaining mobility to the extent that it fully dissolves in water and transforms into a highly dynamic random coil. This hydration-induced behavior induces chain dynamics in the glycine-rich amorphous soft segments on the microsecond time scale, contributing to the elasticity of the solid material. Our findings not only reveal the presence of structurally and dynamically distinct segments within the film’s superstructure but also highlight the complexity of the self-organization responsible for the exceptional mechanical properties observed in proteins that mimic dragline silk.","lang":"eng"}],"year":"2024","issue":"3","date_published":"2024-03-11T00:00:00Z","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"corr_author":"1","file_date_updated":"2024-10-07T08:33:35Z","scopus_import":"1","language":[{"iso":"eng"}],"citation":{"mla":"Wu, Dongqing, et al. “Unveiling the Dynamic Self-Assembly of a Recombinant Dragline-Silk-Mimicking Protein.” <i>Biomacromolecules</i>, vol. 25, no. 3, American Chemical Society, 2024, pp. 1759–74, doi:<a href=\"https://doi.org/10.1021/acs.biomac.3c01239\">10.1021/acs.biomac.3c01239</a>.","ieee":"D. Wu <i>et al.</i>, “Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein,” <i>Biomacromolecules</i>, vol. 25, no. 3. American Chemical Society, pp. 1759–1774, 2024.","ista":"Wu D, Koscic A, Schneider S, Dubini RCA, Rodriguez Camargo DC, Schneider S, Rovo P. 2024. Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein. Biomacromolecules. 25(3), 1759–1774.","short":"D. Wu, A. Koscic, S. Schneider, R.C.A. Dubini, D.C. Rodriguez Camargo, S. Schneider, P. Rovo, Biomacromolecules 25 (2024) 1759–1774.","ama":"Wu D, Koscic A, Schneider S, et al. Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein. <i>Biomacromolecules</i>. 2024;25(3):1759-1774. doi:<a href=\"https://doi.org/10.1021/acs.biomac.3c01239\">10.1021/acs.biomac.3c01239</a>","chicago":"Wu, Dongqing, Anamaria Koscic, Sonja Schneider, Romeo C. A. Dubini, Diana C. Rodriguez Camargo, Sabine Schneider, and Petra Rovo. “Unveiling the Dynamic Self-Assembly of a Recombinant Dragline-Silk-Mimicking Protein.” <i>Biomacromolecules</i>. American Chemical Society, 2024. <a href=\"https://doi.org/10.1021/acs.biomac.3c01239\">https://doi.org/10.1021/acs.biomac.3c01239</a>.","apa":"Wu, D., Koscic, A., Schneider, S., Dubini, R. C. A., Rodriguez Camargo, D. C., Schneider, S., &#38; Rovo, P. (2024). Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein. <i>Biomacromolecules</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.biomac.3c01239\">https://doi.org/10.1021/acs.biomac.3c01239</a>"},"has_accepted_license":"1","publication_identifier":{"eissn":["1526-4602"],"issn":["1525-7797"]},"acknowledgement":"We thank Dr. Pavel Kielkowski for performing the MS/MS measurement and providing feedback on the manuscript. We are grateful to Rodrigo Ledesma Amaro for introducing the Golden Gate Assembly technique in our lab. We acknowledge the support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)─SFB 1309-325871075, the Center for NanoScience (CeNS), the Fonds der Chemischen Industrie, and Universitätsgesellschaft München.","page":"1759-1774","file":[{"creator":"dernst","content_type":"application/pdf","date_created":"2024-10-07T08:33:35Z","success":1,"access_level":"open_access","file_id":"18180","file_size":6597227,"checksum":"9552b6d52f1e8a350764849a535fc13e","relation":"main_file","file_name":"2024_BioMacromolecules_Wu.pdf","date_updated":"2024-10-07T08:33:35Z"}],"external_id":{"isi":["001166501000001"],"pmid":["38343096"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"03","type":"journal_article","oa_version":"Published Version","publication_status":"published","doi":"10.1021/acs.biomac.3c01239","status":"public","isi":1,"department":[{"_id":"NMR"}],"date_updated":"2025-09-08T09:52:18Z","article_processing_charge":"Yes (via OA deal)","author":[{"full_name":"Wu, Dongqing","last_name":"Wu","first_name":"Dongqing"},{"first_name":"Anamaria","full_name":"Koscic, Anamaria","last_name":"Koscic"},{"full_name":"Schneider, Sonja","last_name":"Schneider","first_name":"Sonja"},{"first_name":"Romeo C. A.","last_name":"Dubini","full_name":"Dubini, Romeo C. A."},{"first_name":"Diana C.","full_name":"Rodriguez Camargo, Diana C.","last_name":"Rodriguez Camargo"},{"first_name":"Sabine","full_name":"Schneider, Sabine","last_name":"Schneider"},{"last_name":"Rovo","full_name":"Rovo, Petra","first_name":"Petra","orcid":"0000-0001-8729-7326","id":"c316e53f-b965-11eb-b128-bb26acc59c00"}]},{"_id":"18310","day":"01","publisher":"SAGE Publications","pmid":1,"publication":"Alternatives to Laboratory Animals","volume":52,"article_type":"original","title":"Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE)","intvolume":"        52","date_created":"2024-10-13T22:01:51Z","quality_controlled":"1","date_published":"2024-11-01T00:00:00Z","issue":"6","year":"2024","scopus_import":"1","citation":{"chicago":"Kitsara, Maria, Merima Smajlhodžić-Deljo, Lejla Gurbeta Pokvic, Bettina Bert, Nataliia Bubalo, Sevilay Erden, Nuno Henrique Franco, et al. “Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE).” <i>Alternatives to Laboratory Animals</i>. SAGE Publications, 2024. <a href=\"https://doi.org/10.1177/02611929241286024\">https://doi.org/10.1177/02611929241286024</a>.","apa":"Kitsara, M., Smajlhodžić-Deljo, M., Gurbeta Pokvic, L., Bert, B., Bubalo, N., Erden, S., … Neuhaus, W. (2024). Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). <i>Alternatives to Laboratory Animals</i>. SAGE Publications. <a href=\"https://doi.org/10.1177/02611929241286024\">https://doi.org/10.1177/02611929241286024</a>","ama":"Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, et al. Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). <i>Alternatives to Laboratory Animals</i>. 2024;52(6):326-333. doi:<a href=\"https://doi.org/10.1177/02611929241286024\">10.1177/02611929241286024</a>","mla":"Kitsara, Maria, et al. “Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE).” <i>Alternatives to Laboratory Animals</i>, vol. 52, no. 6, SAGE Publications, 2024, pp. 326–33, doi:<a href=\"https://doi.org/10.1177/02611929241286024\">10.1177/02611929241286024</a>.","ista":"Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, Bert B, Bubalo N, Erden S, Franco NH, Chirico G, Gómez Raja J, Gonzalez-Uarquin F, Lang A, Linklater N, Mojsova S, Olsson IAS, Sandvig I, Schaffert A, Schmit M, Schober S, Sevastre B, Wilflingseder D, Ahluwalia A, Neuhaus W. 2024. Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). Alternatives to Laboratory Animals. 52(6), 326–333.","ieee":"M. Kitsara <i>et al.</i>, “Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE),” <i>Alternatives to Laboratory Animals</i>, vol. 52, no. 6. SAGE Publications, pp. 326–333, 2024.","short":"M. Kitsara, M. Smajlhodžić-Deljo, L. Gurbeta Pokvic, B. Bert, N. Bubalo, S. Erden, N.H. Franco, G. Chirico, J. Gómez Raja, F. Gonzalez-Uarquin, A. Lang, N. Linklater, S. Mojsova, I.A.S. Olsson, I. Sandvig, A. Schaffert, M. Schmit, S. Schober, B. Sevastre, D. Wilflingseder, A. Ahluwalia, W. Neuhaus, Alternatives to Laboratory Animals 52 (2024) 326–333."},"language":[{"iso":"eng"}],"OA_type":"closed access","external_id":{"isi":["001348633700007"],"pmid":["39333027"]},"page":"326-333","publication_identifier":{"eissn":["2632-3559"],"issn":["0261-1929"]},"month":"11","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.1177/02611929241286024","publication_status":"published","oa_version":"None","type":"journal_article","isi":1,"status":"public","date_updated":"2025-09-08T09:56:39Z","department":[{"_id":"PreCl"}],"article_processing_charge":"No","author":[{"first_name":"Maria","last_name":"Kitsara","full_name":"Kitsara, Maria"},{"full_name":"Smajlhodžić-Deljo, Merima","last_name":"Smajlhodžić-Deljo","first_name":"Merima"},{"first_name":"Lejla","full_name":"Gurbeta Pokvic, Lejla","last_name":"Gurbeta Pokvic"},{"last_name":"Bert","full_name":"Bert, Bettina","first_name":"Bettina"},{"first_name":"Nataliia","last_name":"Bubalo","full_name":"Bubalo, Nataliia"},{"last_name":"Erden","full_name":"Erden, Sevilay","first_name":"Sevilay"},{"full_name":"Franco, Nuno Henrique","last_name":"Franco","first_name":"Nuno Henrique"},{"last_name":"Chirico","full_name":"Chirico, Giuseppe","first_name":"Giuseppe"},{"full_name":"Gómez Raja, Jonathan","last_name":"Gómez Raja","first_name":"Jonathan"},{"last_name":"Gonzalez-Uarquin","full_name":"Gonzalez-Uarquin, Fernando","first_name":"Fernando"},{"first_name":"Annemarie","full_name":"Lang, Annemarie","last_name":"Lang"},{"first_name":"Nicole","last_name":"Linklater","full_name":"Linklater, Nicole"},{"first_name":"Sandra","full_name":"Mojsova, Sandra","last_name":"Mojsova"},{"first_name":"I. Anna S.","full_name":"Olsson, I. Anna S.","last_name":"Olsson"},{"first_name":"Ioanna","full_name":"Sandvig, Ioanna","last_name":"Sandvig"},{"full_name":"Schaffert, Alexandra","last_name":"Schaffert","first_name":"Alexandra"},{"last_name":"Schmit","full_name":"Schmit, Marthe","first_name":"Marthe"},{"full_name":"Schober, Sophie","last_name":"Schober","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","first_name":"Sophie"},{"first_name":"Bogdan","full_name":"Sevastre, Bogdan","last_name":"Sevastre"},{"full_name":"Wilflingseder, Doris","last_name":"Wilflingseder","first_name":"Doris"},{"first_name":"Arti","last_name":"Ahluwalia","full_name":"Ahluwalia, Arti"},{"full_name":"Neuhaus, Winfried","last_name":"Neuhaus","first_name":"Winfried"}]},{"scopus_import":"1","file_date_updated":"2024-12-10T08:28:17Z","citation":{"apa":"Marolt Presen, D., Goeschl, V., Hanetseder, D., Ogrin, L., Stetco, A. L., Tansek, A., … Redl, H. (2024). Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium. <i>Stem Cell Research and Therapy</i>. Springer Nature. <a href=\"https://doi.org/10.1186/s13287-024-03960-5\">https://doi.org/10.1186/s13287-024-03960-5</a>","chicago":"Marolt Presen, Darja, Vanessa Goeschl, Dominik Hanetseder, Laura Ogrin, Alexandra Larissa Stetco, Anja Tansek, Laura Pozenel, et al. “Prolonged Cultivation Enhances the Stimulatory Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned Medium.” <i>Stem Cell Research and Therapy</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1186/s13287-024-03960-5\">https://doi.org/10.1186/s13287-024-03960-5</a>.","ama":"Marolt Presen D, Goeschl V, Hanetseder D, et al. Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium. <i>Stem Cell Research and Therapy</i>. 2024;15. doi:<a href=\"https://doi.org/10.1186/s13287-024-03960-5\">10.1186/s13287-024-03960-5</a>","ieee":"D. Marolt Presen <i>et al.</i>, “Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium,” <i>Stem Cell Research and Therapy</i>, vol. 15. Springer Nature, 2024.","short":"D. Marolt Presen, V. Goeschl, D. Hanetseder, L. Ogrin, A.L. Stetco, A. Tansek, L. Pozenel, B. Bruszel, G. Mitulovic, J. Oesterreicher, J. Zipperle, B. Schaedl, W. Holnthoner, J. Grillari, H. Redl, Stem Cell Research and Therapy 15 (2024).","ista":"Marolt Presen D, Goeschl V, Hanetseder D, Ogrin L, Stetco AL, Tansek A, Pozenel L, Bruszel B, Mitulovic G, Oesterreicher J, Zipperle J, Schaedl B, Holnthoner W, Grillari J, Redl H. 2024. Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium. Stem Cell Research and Therapy. 15, 434.","mla":"Marolt Presen, Darja, et al. “Prolonged Cultivation Enhances the Stimulatory Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned Medium.” <i>Stem Cell Research and Therapy</i>, vol. 15, 434, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1186/s13287-024-03960-5\">10.1186/s13287-024-03960-5</a>."},"has_accepted_license":"1","article_number":"434","language":[{"iso":"eng"}],"acknowledgement":"We thank the personnel of the Lorenz-Böhler-Unfallkrankenhaus for providing the human tissue waste for primary cell isolation and the New York Stem Cell Foundation Research Institute for providing the human induced pluripotent stem cell line 1013 A and its mesenchymal progenitors. We also thank all our colleagues at the Ludwig Boltzmann Institute for Traumatology for their suggestions and ongoing support of the project. InstaText writing tool (https://instatext.io) was used to edit the English language of the final manuscript.\r\nThis work has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie actions (grant agreement No. 657716) and the Transforming European Industry call H2020-NMBP-TRIND-2020 (grant agreement No. 953134), as well as by the FFG Industrienahe Dissertation program (grant agreement No. 867803 and 853056), the FEMtech Praktika program (grant agreement No. 852154, 868917 and 877951) and the Production of the Future program (grant agreement No. 877452).","file":[{"content_type":"application/pdf","creator":"dernst","file_id":"18641","date_created":"2024-12-10T08:28:17Z","success":1,"access_level":"open_access","checksum":"91edba8edde30d781dce89fdd5cadc39","relation":"main_file","file_size":6690494,"date_updated":"2024-12-10T08:28:17Z","file_name":"2024_StemCellResearch_Presen.pdf"}],"external_id":{"pmid":["39551765"],"isi":["001356479400001"]},"OA_type":"gold","publication_identifier":{"eissn":["1757-6512"]},"month":"12","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","DOAJ_listed":"1","publication_status":"published","doi":"10.1186/s13287-024-03960-5","type":"journal_article","oa_version":"Published Version","isi":1,"status":"public","date_updated":"2025-09-09T11:41:12Z","department":[{"_id":"LifeSc"}],"article_processing_charge":"Yes","author":[{"first_name":"Darja","last_name":"Marolt Presen","full_name":"Marolt Presen, Darja"},{"first_name":"Vanessa","last_name":"Goeschl","full_name":"Goeschl, Vanessa"},{"first_name":"Dominik","last_name":"Hanetseder","full_name":"Hanetseder, Dominik"},{"full_name":"Ogrin, Laura","last_name":"Ogrin","first_name":"Laura"},{"first_name":"Alexandra Larissa","full_name":"Stetco, Alexandra Larissa","last_name":"Stetco"},{"first_name":"Anja","full_name":"Tansek, Anja","last_name":"Tansek"},{"first_name":"Laura","last_name":"Pozenel","full_name":"Pozenel, Laura"},{"full_name":"Bruszel, Bella","last_name":"Bruszel","id":"70abbbb3-88ea-11ec-8e0a-e8c939944834","first_name":"Bella"},{"first_name":"Goran","last_name":"Mitulovic","full_name":"Mitulovic, Goran"},{"first_name":"Johannes","last_name":"Oesterreicher","full_name":"Oesterreicher, Johannes"},{"first_name":"Johannes","last_name":"Zipperle","full_name":"Zipperle, Johannes"},{"first_name":"Barbara","full_name":"Schaedl, Barbara","last_name":"Schaedl"},{"first_name":"Wolfgang","last_name":"Holnthoner","full_name":"Holnthoner, Wolfgang"},{"full_name":"Grillari, Johannes","last_name":"Grillari","first_name":"Johannes"},{"last_name":"Redl","full_name":"Redl, Heinz","first_name":"Heinz"}],"_id":"18581","publisher":"Springer Nature","day":"01","ddc":["570"],"pmid":1,"publication":"Stem Cell Research and Therapy","OA_place":"publisher","volume":15,"article_type":"original","title":"Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium","date_created":"2024-11-24T23:01:47Z","intvolume":"        15","abstract":[{"lang":"eng","text":"Background: Human induced pluripotent stem cells represent a scalable source of youthful tissue progenitors and secretomes for regenerative therapies. The aim of our study was to investigate the potential of conditioned medium (CM) from hiPSC-mesenchymal progenitors (hiPSC-MPs) to stimulate osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells (MSCs). We also investigated whether prolonged cultivation or osteogenic pre-differentiation of hiPSC-MPs could enhance the stimulatory activity of CM.\r\nMethods: MSCs were isolated from 13 donors (age 20–90 years). CM derived from hiPSC-MPs was added to the MSC cultures and the effects on proliferation and osteogenic differentiation were examined after 14 days and 6 weeks. The stimulatory activity of hiPSC-MP-CM was compared with the activity of MSC-derived CM and with the activity of CM prepared from hiPSC-MPs pre-cultured in growth or osteogenic medium for 14 days. Comparative proteomic analysis of CM was performed to gain insight into the molecular components responsible for the stimulatory activity.\r\nResults: Primary bone marrow-derived MSC exhibited variability, with a tendency towards lower proliferation and tri-lineage differentiation in older donors. hiPSC-MP-CM increased the proliferation and alkaline phosphatase activity of MSC from several adult/aged donors after 14 days of continuous supplementation under osteogenic conditions. However, CM supplementation failed to improve the mineralization of MSC pellets after 6 weeks under osteogenic conditions. hiPSC-MP-CM showed greater enhancement of proliferation and ALP activity than CM derived from bone marrow-derived MSCs. Moreover, 14-day cultivation but not osteogenic pre-differentiation of hiPSC-MPs strongly enhanced CM stimulatory activity. Quantitative proteomic analysis of d14-CM revealed a distinct profile of components that formed a highly interconnected associations network with two clusters, one functionally associated with binding and organization of actin/cytoskeletal components and the other with structural constituents of the extracellular matrix, collagen, and growth factor binding. Several hub proteins were identified that were reported to have functions in cell-extracellular matrix interaction, osteogenic differentiation and development.\r\nConclusions: Our data show that hiPSC-MP-CM enhances early osteogenic differentiation of human bone marrow-derived MSCs and that prolonged cultivation of hiPSC-MPs enhances CM-stimulatory activity. Proteomic analysis of the upregulated protein components provides the basis for further optimization of hiPSC-MP-CM for bone regenerative therapies."}],"quality_controlled":"1","date_published":"2024-12-01T00:00:00Z","year":"2024","tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"oa":1},{"article_processing_charge":"No","author":[{"first_name":"Jan","last_name":"Lauwereyns","full_name":"Lauwereyns, Jan"},{"full_name":"Bajramovic, Jeffrey","last_name":"Bajramovic","first_name":"Jeffrey"},{"first_name":"Bettina","last_name":"Bert","full_name":"Bert, Bettina"},{"full_name":"Camenzind, Samuel","last_name":"Camenzind","first_name":"Samuel"},{"first_name":"Joery","last_name":"De Kock","full_name":"De Kock, Joery"},{"first_name":"Alisa","full_name":"Elezović, Alisa","last_name":"Elezović"},{"first_name":"Sevilay","last_name":"Erden","full_name":"Erden, Sevilay"},{"first_name":"Fernando","last_name":"Gonzalez-Uarquin","full_name":"Gonzalez-Uarquin, Fernando"},{"last_name":"Ulman","full_name":"Ulman, Yesim Isil","first_name":"Yesim Isil"},{"first_name":"Orsolya Ivett","full_name":"Hoffmann, Orsolya Ivett","last_name":"Hoffmann"},{"first_name":"Maria","last_name":"Kitsara","full_name":"Kitsara, Maria"},{"full_name":"Kostomitsopoulos, Nikolaos","last_name":"Kostomitsopoulos","first_name":"Nikolaos"},{"full_name":"Neuhaus, Winfried","last_name":"Neuhaus","first_name":"Winfried"},{"full_name":"Petit-Demouliere, Benoit","last_name":"Petit-Demouliere","first_name":"Benoit"},{"first_name":"Simone","last_name":"Pollo","full_name":"Pollo, Simone"},{"first_name":"Brígida","full_name":"Riso, Brígida","last_name":"Riso"},{"last_name":"Schober","full_name":"Schober, Sophie","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","first_name":"Sophie"},{"full_name":"Sotiropoulos, Athanassia","last_name":"Sotiropoulos","first_name":"Athanassia"},{"first_name":"Aurélie","last_name":"Thomas","full_name":"Thomas, Aurélie"},{"first_name":"Augusto","last_name":"Vitale","full_name":"Vitale, Augusto"},{"last_name":"Wilflingseder","full_name":"Wilflingseder, Doris","first_name":"Doris"},{"first_name":"Arti","last_name":"Ahluwalia","full_name":"Ahluwalia, Arti"}],"date_updated":"2025-09-08T14:50:31Z","department":[{"_id":"PreCl"}],"isi":1,"status":"public","doi":"10.1038/s41684-024-01476-2","publication_status":"published","oa_version":"Published Version","type":"journal_article","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"12","OA_type":"hybrid","external_id":{"isi":["001355264100001"],"pmid":["39548348"]},"file":[{"content_type":"application/pdf","creator":"dernst","file_id":"18614","success":1,"access_level":"open_access","date_created":"2024-12-03T14:07:04Z","relation":"main_file","checksum":"67fc140f761581a291591f075e49b88d","file_size":967252,"file_name":"2024_LabAnimal_Lauwereyns.pdf","date_updated":"2024-12-03T14:07:04Z"}],"acknowledgement":"This publication is based upon work from the Ethics Crossover Group within the COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”), CA21139, supported by COST (European Cooperation in Science and Technology). We acknowledge the input and advice from Dr. Susanna Louhimies.","page":"347-350","publication_identifier":{"issn":["0093-7355"],"eissn":["1548-4475"]},"has_accepted_license":"1","citation":{"mla":"Lauwereyns, Jan, et al. “Toward a Common Interpretation of the 3Rs Principles in Animal Research.” <i>Lab Animal</i>, vol. 53, Springer Nature, 2024, pp. 347–50, doi:<a href=\"https://doi.org/10.1038/s41684-024-01476-2\">10.1038/s41684-024-01476-2</a>.","ista":"Lauwereyns J, Bajramovic J, Bert B, Camenzind S, De Kock J, Elezović A, Erden S, Gonzalez-Uarquin F, Ulman YI, Hoffmann OI, Kitsara M, Kostomitsopoulos N, Neuhaus W, Petit-Demouliere B, Pollo S, Riso B, Schober S, Sotiropoulos A, Thomas A, Vitale A, Wilflingseder D, Ahluwalia A. 2024. Toward a common interpretation of the 3Rs principles in animal research. Lab Animal. 53, 347–350.","ieee":"J. Lauwereyns <i>et al.</i>, “Toward a common interpretation of the 3Rs principles in animal research,” <i>Lab Animal</i>, vol. 53. Springer Nature, pp. 347–350, 2024.","short":"J. Lauwereyns, J. Bajramovic, B. Bert, S. Camenzind, J. De Kock, A. Elezović, S. Erden, F. Gonzalez-Uarquin, Y.I. Ulman, O.I. Hoffmann, M. Kitsara, N. Kostomitsopoulos, W. Neuhaus, B. Petit-Demouliere, S. Pollo, B. Riso, S. Schober, A. Sotiropoulos, A. Thomas, A. Vitale, D. Wilflingseder, A. Ahluwalia, Lab Animal 53 (2024) 347–350.","chicago":"Lauwereyns, Jan, Jeffrey Bajramovic, Bettina Bert, Samuel Camenzind, Joery De Kock, Alisa Elezović, Sevilay Erden, et al. “Toward a Common Interpretation of the 3Rs Principles in Animal Research.” <i>Lab Animal</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41684-024-01476-2\">https://doi.org/10.1038/s41684-024-01476-2</a>.","apa":"Lauwereyns, J., Bajramovic, J., Bert, B., Camenzind, S., De Kock, J., Elezović, A., … Ahluwalia, A. (2024). Toward a common interpretation of the 3Rs principles in animal research. <i>Lab Animal</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41684-024-01476-2\">https://doi.org/10.1038/s41684-024-01476-2</a>","ama":"Lauwereyns J, Bajramovic J, Bert B, et al. Toward a common interpretation of the 3Rs principles in animal research. <i>Lab Animal</i>. 2024;53:347-350. doi:<a href=\"https://doi.org/10.1038/s41684-024-01476-2\">10.1038/s41684-024-01476-2</a>"},"language":[{"iso":"eng"}],"scopus_import":"1","file_date_updated":"2024-12-03T14:07:04Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"oa":1,"date_published":"2024-12-01T00:00:00Z","year":"2024","abstract":[{"text":"Many scientific breakthroughs have depended on animal research, yet the ethical concerns surrounding the use of animals in experimentation have long prompted discussions about humane treatment and responsible scientific practice. First articulated by Russell and Burch, the 3Rs Principles of Replacement, Reduction, and Refinement have gained widespread recognition as basic guidelines for animal research. Over time, the 3Rs have transcended the research community, influencing policy decisions, animal welfare advocacy and public perception of animal experimentation. Despite their broad acceptance, interpretations of the 3Rs vary substantially, shaping statutory frameworks at various levels, with both technical and practical impacts.","lang":"eng"}],"intvolume":"        53","date_created":"2024-11-24T23:01:49Z","quality_controlled":"1","volume":53,"OA_place":"publisher","title":"Toward a common interpretation of the 3Rs principles in animal research","article_type":"letter_note","publication":"Lab Animal","ddc":["570"],"pmid":1,"_id":"18587","day":"01","publisher":"Springer Nature"},{"oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"year":"2024","issue":"15","date_published":"2024-04-01T00:00:00Z","quality_controlled":"1","intvolume":"        10","date_created":"2025-01-27T14:32:34Z","abstract":[{"text":"The globally distributed marine alga Emiliania huxleyi has cooling effect on the Earth’s climate. The population density of E. huxleyi is restricted by Nucleocytoviricota viruses, including E. huxleyi virus 201 (EhV-201). Despite the impact of E. huxleyi viruses on the climate, there is limited information about their structure and replication. Here, we show that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane. EhV-201 virions infect E. huxleyi by using fivefold vertices to bind to and fuse the virus’ inner membrane with the cell plasma membrane. Progeny virions assemble in the cytoplasm at the surface of endoplasmic reticulum–derived membrane segments. Genome packaging initiates synchronously with the capsid assembly and completes through an aperture in the forming capsid. The genome-filled capsids acquire an outer membrane by budding into intracellular vesicles. EhV-201 infection induces a loss of surface protective layers from E. huxleyi cells, which enables the continuous release of virions by exocytosis.","lang":"eng"}],"title":"Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi","article_type":"original","OA_place":"publisher","volume":10,"publication":"Science Advances","pmid":1,"ddc":["570"],"publisher":"American Association for the Advancement of Science","day":"01","_id":"18920","article_processing_charge":"Yes","author":[{"first_name":"Miroslav","last_name":"Homola","full_name":"Homola, Miroslav"},{"first_name":"Renate Carina","id":"3b7984c9-17ff-11ed-b6fe-f943c4a5b626","last_name":"Büttner","full_name":"Büttner, Renate Carina"},{"full_name":"Füzik, Tibor","last_name":"Füzik","first_name":"Tibor"},{"full_name":"Křepelka, Pavel","last_name":"Křepelka","first_name":"Pavel"},{"last_name":"Holbová","full_name":"Holbová, Radka","first_name":"Radka"},{"first_name":"Jiří","last_name":"Nováček","full_name":"Nováček, Jiří"},{"full_name":"Chaillet, Marten L.","last_name":"Chaillet","first_name":"Marten L."},{"last_name":"Žák","full_name":"Žák, Jakub","first_name":"Jakub"},{"first_name":"Danyil","full_name":"Grybchuk, Danyil","last_name":"Grybchuk"},{"last_name":"Förster","full_name":"Förster, Friedrich","first_name":"Friedrich"},{"first_name":"William H.","full_name":"Wilson, William H.","last_name":"Wilson"},{"last_name":"Schroeder","full_name":"Schroeder, Declan C.","first_name":"Declan C."},{"first_name":"Pavel","full_name":"Plevka, Pavel","last_name":"Plevka"}],"department":[{"_id":"EM-Fac"}],"date_updated":"2025-05-14T09:29:04Z","status":"public","type":"journal_article","oa_version":"Published Version","publication_status":"published","doi":"10.1126/sciadv.adk1954","DOAJ_listed":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"04","publication_identifier":{"eissn":["2375-2548"]},"related_material":{"link":[{"relation":"software","url":" https://github.com/fuzikt/tomostarpy."}]},"file":[{"access_level":"open_access","success":1,"date_created":"2025-01-27T14:40:08Z","file_id":"18921","content_type":"application/pdf","creator":"dernst","file_name":"2024_ScienceAdv_Homola.pdf","date_updated":"2025-01-27T14:40:08Z","file_size":40623405,"relation":"main_file","checksum":"291dd7ceccbe6bfd8e0a9157584f88e9"}],"acknowledgement":"We acknowledge (i) the Cryo-Electron Microscopy and Tomography Core Facility and Proteomics Core Facility of the Central European Institute of Technology (CEITEC), Masaryk University, supported by the Ministry of Education, Youth, and Sports of the Czech Republic (grant LM2018127); (ii) the Cellular Imaging Core Facility supported by the Czech-BioImaging large RI project (LM2018129 funded by MEYS CR); and (iii) Plant Sciences Core Facility for support with obtaining scientific data presented here. We acknowledge support from the project National Institute of Virology and Bacteriology (Program EXCELES, ID project no. LX22NPO5103), funded by the European Union - Next Generation EU. This work received funding from the Czech Science Foundation grant GX 19-259882X to P.P., from European Regional Development Fund-Project “MSCAfellow2@MUNI” (no. CZ.02.2.69/0.0/0.0/18_070/0009846) to C.R.B., and from Brno PhD talent scholarship funded by Brno city municipality to M.H.","OA_type":"gold","external_id":{"pmid":["38598627"]},"language":[{"iso":"eng"}],"citation":{"ama":"Homola M, Büttner RC, Füzik T, et al. Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi. <i>Science Advances</i>. 2024;10(15). doi:<a href=\"https://doi.org/10.1126/sciadv.adk1954\">10.1126/sciadv.adk1954</a>","chicago":"Homola, Miroslav, Renate Carina Büttner, Tibor Füzik, Pavel Křepelka, Radka Holbová, Jiří Nováček, Marten L. Chaillet, et al. “Structure and Replication Cycle of a Virus Infecting Climate-Modulating Alga Emiliania Huxleyi.” <i>Science Advances</i>. American Association for the Advancement of Science, 2024. <a href=\"https://doi.org/10.1126/sciadv.adk1954\">https://doi.org/10.1126/sciadv.adk1954</a>.","apa":"Homola, M., Büttner, R. C., Füzik, T., Křepelka, P., Holbová, R., Nováček, J., … Plevka, P. (2024). Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.adk1954\">https://doi.org/10.1126/sciadv.adk1954</a>","mla":"Homola, Miroslav, et al. “Structure and Replication Cycle of a Virus Infecting Climate-Modulating Alga Emiliania Huxleyi.” <i>Science Advances</i>, vol. 10, no. 15, eadk1954, American Association for the Advancement of Science, 2024, doi:<a href=\"https://doi.org/10.1126/sciadv.adk1954\">10.1126/sciadv.adk1954</a>.","ieee":"M. Homola <i>et al.</i>, “Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi,” <i>Science Advances</i>, vol. 10, no. 15. American Association for the Advancement of Science, 2024.","short":"M. Homola, R.C. Büttner, T. Füzik, P. Křepelka, R. Holbová, J. Nováček, M.L. Chaillet, J. Žák, D. Grybchuk, F. Förster, W.H. Wilson, D.C. Schroeder, P. Plevka, Science Advances 10 (2024).","ista":"Homola M, Büttner RC, Füzik T, Křepelka P, Holbová R, Nováček J, Chaillet ML, Žák J, Grybchuk D, Förster F, Wilson WH, Schroeder DC, Plevka P. 2024. Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi. Science Advances. 10(15), eadk1954."},"has_accepted_license":"1","article_number":"eadk1954 ","file_date_updated":"2025-01-27T14:40:08Z","scopus_import":"1"}]