--- DOAJ_listed: '1' OA_place: publisher OA_type: gold PlanS_conform: '1' _id: '21231' abstract: - lang: eng text: To assess cell migration in complex spatial environments, microfabricated chips, such as mazes and pillar forests, are routinely used to impose spatial and mechanical constraints, and cell trajectories are followed within these structures by advanced imaging techniques. In systems mechanobiology, computational models serve as essential tools to uncover how physical geometry influences intracellular dynamics; however, decoding such complex behaviors requires advanced inference techniques. Here, we integrated experimental observations of dendritic cell migration in a geometrically constrained microenvironment into a Cellular Potts model. We demonstrated that these spatial constraints modulate the motility dynamics, including speed and directional changes. We show that classical summary statistics, such as mean squared displacement and turning angle distributions, can resolve key mechanistic features but fail to extract richer spatiotemporal patterns, limiting accurate parameter inference. To solve this, we applied neural posterior estimation with in-the-loop learning of summary features. This learned summary representation of the data enables robust and flexible parameter inference, providing a data-driven framework for model calibration and advancing quantitative analysis of cell migration in structured microenvironments. acknowledgement: 'This work was supported by the German Federal Ministry of Education and Research (BMBF) (EMUNE/031L0293C), the European Union via the ERC grant INTEGRATE, grant agreement number 101126146, and under Germany’s Excellence Strategy by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) (EXC 2047—390685813, EXC 2151—390873048, FOR5775 — 533863915, and 524747443), the University of Bonn via the Schlegel Professorship of J.H., and the returning experts fellowship of the Ministry of Innovation, Science, and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069). J.M. is a member of the Nanofabrication Facility and is supported by the Institute of Science and Technology Austria. E.K. acknowledges the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments. The authors thank Laeschkir Würthner for his insightful comments on the implementation of the authors’ model. The views and opinions expressed are those of the authors only and do not necessarily reflect those of the funding agencies. Parts of Fig. 1 were created using BioRender. Open Access funding enabled and organized by Projekt DEAL.' article_number: '20' article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Jonas full_name: Arruda, Jonas last_name: Arruda - first_name: Emad full_name: Alamoudi, Emad last_name: Alamoudi - first_name: Robert full_name: Mueller, Robert last_name: Mueller - first_name: Marc full_name: Vaisband, Marc last_name: Vaisband - first_name: Ronja full_name: Molkenbur, Ronja last_name: Molkenbur - first_name: Jack full_name: Merrin, Jack id: 4515C308-F248-11E8-B48F-1D18A9856A87 last_name: Merrin orcid: 0000-0001-5145-4609 - first_name: Eva full_name: Kiermaier, Eva last_name: Kiermaier - first_name: Jan full_name: Hasenauer, Jan last_name: Hasenauer citation: ama: Arruda J, Alamoudi E, Mueller R, et al. Simulation-based inference of cell migration dynamics in complex spatial environments. npj Systems Biology and Applications. 2026;12. doi:10.1038/s41540-026-00648-9 apa: Arruda, J., Alamoudi, E., Mueller, R., Vaisband, M., Molkenbur, R., Merrin, J., … Hasenauer, J. (2026). Simulation-based inference of cell migration dynamics in complex spatial environments. Npj Systems Biology and Applications. Springer Nature. https://doi.org/10.1038/s41540-026-00648-9 chicago: Arruda, Jonas, Emad Alamoudi, Robert Mueller, Marc Vaisband, Ronja Molkenbur, Jack Merrin, Eva Kiermaier, and Jan Hasenauer. “Simulation-Based Inference of Cell Migration Dynamics in Complex Spatial Environments.” Npj Systems Biology and Applications. Springer Nature, 2026. https://doi.org/10.1038/s41540-026-00648-9. ieee: J. Arruda et al., “Simulation-based inference of cell migration dynamics in complex spatial environments,” npj Systems Biology and Applications, vol. 12. Springer Nature, 2026. ista: Arruda J, Alamoudi E, Mueller R, Vaisband M, Molkenbur R, Merrin J, Kiermaier E, Hasenauer J. 2026. Simulation-based inference of cell migration dynamics in complex spatial environments. npj Systems Biology and Applications. 12, 20. mla: Arruda, Jonas, et al. “Simulation-Based Inference of Cell Migration Dynamics in Complex Spatial Environments.” Npj Systems Biology and Applications, vol. 12, 20, Springer Nature, 2026, doi:10.1038/s41540-026-00648-9. short: J. Arruda, E. Alamoudi, R. Mueller, M. Vaisband, R. Molkenbur, J. Merrin, E. Kiermaier, J. Hasenauer, Npj Systems Biology and Applications 12 (2026). date_created: 2026-02-16T10:44:31Z date_published: 2026-02-05T00:00:00Z date_updated: 2026-02-23T10:10:10Z day: '05' ddc: - '570' department: - _id: NanoFab doi: 10.1038/s41540-026-00648-9 external_id: pmid: - '41611727' file: - access_level: open_access checksum: 99b2e6bbaaedf45f22e07751948669f5 content_type: application/pdf creator: dernst date_created: 2026-02-23T10:09:03Z date_updated: 2026-02-23T10:09:03Z file_id: '21346' file_name: 2026_npjSysBioApp_Arruda.pdf file_size: 10217687 relation: main_file success: 1 file_date_updated: 2026-02-23T10:09:03Z has_accepted_license: '1' intvolume: ' 12' language: - iso: eng month: '02' oa: 1 oa_version: Published Version pmid: 1 publication: npj Systems Biology and Applications publication_identifier: eissn: - 2056-7189 publication_status: published publisher: Springer Nature quality_controlled: '1' scopus_import: '1' status: public title: Simulation-based inference of cell migration dynamics in complex spatial environments tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 12 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold PlanS_conform: '1' _id: '21382' abstract: - lang: eng text: The exceptional energy-harvesting efficiency of lead-halide perovskites arises from unusually long photocarrier diffusion lengths and recombination lifetimes that persist even in defect-rich, solution-grown samples. Paradoxically, perovskites are also known for having very short exciton decay times. Here, we resolve this apparent contradiction by showing that key optoelectronic properties of perovskites can be explained by localized flexoelectric polarization confined to interfaces between domains of spontaneous strain. Using birefringence imaging, electrochemical staining, and zero-bias photocurrent measurements, we visualize the domain structure and directly probe the associated internal fields in nominally cubic single crystals of methylammonium lead bromide. We demonstrate that localized flexoelectric fields spatially separate electrons and holes to opposite sides of domain walls, exponentially suppressing recombination. Domain walls thus act as efficient mesoscopic transport channels for long-lived photocarriers, microscopically linking structural heterogeneity to charge transport and offering mechanistically informed design principles for perovskite solar-energy technologies. acknowledged_ssus: - _id: Bio - _id: M-Shop acknowledgement: We are grateful to A. G. Volosniev for the valuable discussions. We thank D. Milius for the assistance with microscopy. D. R. would like to thank F. Filakovský and T. Čuchráč for the valuable discussions. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Miba Machine Shop Facility (MS). article_number: '946' article_processing_charge: Yes article_type: original author: - first_name: Dmytro full_name: Rak, Dmytro id: 70313b46-47c2-11ec-9e88-cd79101918fe last_name: Rak - first_name: Dusan full_name: Lorenc, Dusan id: 40D8A3E6-F248-11E8-B48F-1D18A9856A87 last_name: Lorenc - first_name: Daniel full_name: Balazs, Daniel id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E last_name: Balazs orcid: 0000-0001-7597-043X - first_name: Ayan A. full_name: Zhumekenov, Ayan A. last_name: Zhumekenov - first_name: Osman M. full_name: Bakr, Osman M. last_name: Bakr - first_name: Zhanybek full_name: Alpichshev, Zhanybek id: 45E67A2A-F248-11E8-B48F-1D18A9856A87 last_name: Alpichshev orcid: 0000-0002-7183-5203 citation: ama: Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. Flexoelectric domain walls enable charge separation and transport in cubic perovskites. Nature Communications. 2026;17. doi:10.1038/s41467-026-68660-5 apa: Rak, D., Lorenc, D., Balazs, D., Zhumekenov, A. A., Bakr, O. M., & Alpichshev, Z. (2026). Flexoelectric domain walls enable charge separation and transport in cubic perovskites. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-026-68660-5 chicago: Rak, Dmytro, Dusan Lorenc, Daniel Balazs, Ayan A. Zhumekenov, Osman M. Bakr, and Zhanybek Alpichshev. “Flexoelectric Domain Walls Enable Charge Separation and Transport in Cubic Perovskites.” Nature Communications. Springer Nature, 2026. https://doi.org/10.1038/s41467-026-68660-5. ieee: D. Rak, D. Lorenc, D. Balazs, A. A. Zhumekenov, O. M. Bakr, and Z. Alpichshev, “Flexoelectric domain walls enable charge separation and transport in cubic perovskites,” Nature Communications, vol. 17. Springer Nature, 2026. ista: Rak D, Lorenc D, Balazs D, Zhumekenov AA, Bakr OM, Alpichshev Z. 2026. Flexoelectric domain walls enable charge separation and transport in cubic perovskites. Nature Communications. 17, 946. mla: Rak, Dmytro, et al. “Flexoelectric Domain Walls Enable Charge Separation and Transport in Cubic Perovskites.” Nature Communications, vol. 17, 946, Springer Nature, 2026, doi:10.1038/s41467-026-68660-5. short: D. Rak, D. Lorenc, D. Balazs, A.A. Zhumekenov, O.M. Bakr, Z. Alpichshev, Nature Communications 17 (2026). corr_author: '1' date_created: 2026-03-02T10:06:58Z date_published: 2026-02-16T00:00:00Z date_updated: 2026-04-28T12:12:46Z day: '16' ddc: - '530' department: - _id: ZhAl - _id: LifeSc doi: 10.1038/s41467-026-68660-5 external_id: pmid: - '41698893' file: - access_level: open_access checksum: dd7a98de892d0b5abefca7e290ca0f77 content_type: application/pdf creator: dernst date_created: 2026-03-02T14:27:56Z date_updated: 2026-03-02T14:27:56Z file_id: '21390' file_name: 2026_NatureComm_Rak.pdf file_size: 2570918 relation: main_file success: 1 file_date_updated: 2026-03-02T14:27:56Z has_accepted_license: '1' intvolume: ' 17' language: - iso: eng month: '02' oa: 1 oa_version: Published Version pmid: 1 publication: Nature Communications publication_identifier: eissn: - 2041-1723 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: link: - description: News on ISTA website relation: press_release url: https://ista.ac.at/en/news/explaining-next-generation-solar-cells/ scopus_import: '1' status: public title: Flexoelectric domain walls enable charge separation and transport in cubic perovskites tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 17 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '21485' abstract: - lang: eng text: Insulating oxides are among the most abundant solid materials in the universe1,2,3. Of the many ways in which they influence natural phenomena, perhaps the most consequential is their capacity to transfer electrical charge during contact4,5,6,7,8,9,10—which occurs even between samples of the same oxide—yet the symmetry-breaking parameter that causes this remains unidentified11,12. Here we show that adventitious carbonaceous molecules adsorbed from the environment are the symmetry-breaking factor in same-material oxide contact electrification (CE). We use acoustic levitation to measure charge exchange between a sphere and a plate composed of identical amorphous silicon dioxide (SiO2). Although charging polarity is random for co-prepared samples, we control it with baking or plasma treatment. Observing the charge-exchange relaxation afterwards, we see dynamics over a timescale of hours and connect this directly to the presence of adventitious carbon with time-of-flight mass spectrometry, low-energy ion scattering and infrared spectroscopy. Going further, we confirm that adventitious carbon can even determine charge exchange among different oxides. Our results identify the symmetry-breaking parameter that causes insulating oxides to exchange charge in settings ranging from desert sands4 to volcanic plumes5,6, while simultaneously highlighting an overlooked factor in CE more broadly. acknowledged_ssus: - _id: M-Shop - _id: NanoFab - _id: ScienComp - _id: LifeSc acknowledgement: This project has received support from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 949120) and from the Marie Skłodowska-Curie programme (grant agreement no. 754411). We acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. N.M. acknowledges support from grant Fondecyt 1221597. G.G. is a Serra Húnter fellow. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility and Lab Support Facility. We thank the Modic group for the use of the Laue camera, T. Zauner for the photography of the experimental set-up and R. Möller for insightful discussions. Open access funding provided by Institute of Science and Technology (IST Austria). article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Galien M full_name: Grosjean, Galien M id: 0C5FDA4A-9CF6-11E9-8939-FF05E6697425 last_name: Grosjean orcid: 0000-0001-5154-417X - first_name: Markus full_name: Ostermann, Markus last_name: Ostermann - first_name: Markus full_name: Sauer, Markus last_name: Sauer - first_name: Michael full_name: Hahn, Michael last_name: Hahn - first_name: Christian M. full_name: Pichler, Christian M. last_name: Pichler - first_name: Florian full_name: Fahrnberger, Florian last_name: Fahrnberger - first_name: Felix full_name: Pertl, Felix id: 6313aec0-15b2-11ec-abd3-ed67d16139af last_name: Pertl orcid: 0000-0003-0463-5794 - first_name: Daniel full_name: Balazs, Daniel id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E last_name: Balazs orcid: 0000-0001-7597-043X - first_name: Mason M. full_name: Link, Mason M. last_name: Link - first_name: Seong H. full_name: Kim, Seong H. last_name: Kim - first_name: Devin L. full_name: Schrader, Devin L. last_name: Schrader - first_name: Adriana full_name: Blanco, Adriana last_name: Blanco - first_name: Francisco full_name: Gracia, Francisco last_name: Gracia - first_name: Nicolás full_name: Mujica, Nicolás last_name: Mujica - first_name: Scott R full_name: Waitukaitis, Scott R id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87 last_name: Waitukaitis orcid: 0000-0002-2299-3176 citation: ama: Grosjean GM, Ostermann M, Sauer M, et al. Adventitious carbon breaks symmetry in oxide contact electrification. Nature. 2026;651(8106):626-631. doi:10.1038/s41586-025-10088-w apa: Grosjean, G. M., Ostermann, M., Sauer, M., Hahn, M., Pichler, C. M., Fahrnberger, F., … Waitukaitis, S. R. (2026). Adventitious carbon breaks symmetry in oxide contact electrification. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-10088-w chicago: Grosjean, Galien M, Markus Ostermann, Markus Sauer, Michael Hahn, Christian M. Pichler, Florian Fahrnberger, Felix Pertl, et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” Nature. Springer Nature, 2026. https://doi.org/10.1038/s41586-025-10088-w. ieee: G. M. Grosjean et al., “Adventitious carbon breaks symmetry in oxide contact electrification,” Nature, vol. 651, no. 8106. Springer Nature, pp. 626–631, 2026. ista: Grosjean GM, Ostermann M, Sauer M, Hahn M, Pichler CM, Fahrnberger F, Pertl F, Balazs D, Link MM, Kim SH, Schrader DL, Blanco A, Gracia F, Mujica N, Waitukaitis SR. 2026. Adventitious carbon breaks symmetry in oxide contact electrification. Nature. 651(8106), 626–631. mla: Grosjean, Galien M., et al. “Adventitious Carbon Breaks Symmetry in Oxide Contact Electrification.” Nature, vol. 651, no. 8106, Springer Nature, 2026, pp. 626–31, doi:10.1038/s41586-025-10088-w. short: G.M. Grosjean, M. Ostermann, M. Sauer, M. Hahn, C.M. Pichler, F. Fahrnberger, F. Pertl, D. Balazs, M.M. Link, S.H. Kim, D.L. Schrader, A. Blanco, F. Gracia, N. Mujica, S.R. Waitukaitis, Nature 651 (2026) 626–631. corr_author: '1' date_created: 2026-03-23T15:04:00Z date_published: 2026-03-18T00:00:00Z date_updated: 2026-04-28T12:06:01Z day: '18' ddc: - '540' department: - _id: ScWa - _id: GradSch - _id: LifeSc doi: 10.1038/s41586-025-10088-w ec_funded: 1 external_id: pmid: - '41851325' file: - access_level: open_access checksum: dafef9ed575b44be4263e948a47ae056 content_type: application/pdf creator: dernst date_created: 2026-03-24T06:57:08Z date_updated: 2026-03-24T06:57:08Z file_id: '21494' file_name: 2026_Nature_Grosjean.pdf file_size: 12245694 relation: main_file success: 1 file_date_updated: 2026-03-24T06:57:08Z has_accepted_license: '1' intvolume: ' 651' issue: '8106' language: - iso: eng month: '03' oa: 1 oa_version: Published Version page: 626-631 pmid: 1 project: - _id: 0aa60e99-070f-11eb-9043-a6de6bdc3afa call_identifier: H2020 grant_number: '949120' name: 'Tribocharge: a multi-scale approach to an enduring problem in physics' - _id: 260C2330-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '754411' name: ISTplus - Postdoctoral Fellowships publication: Nature publication_identifier: eissn: - 1476-4687 issn: - 0028-0836 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: link: - description: News on ISTA website relation: press_release url: https://ista.ac.at/en/news/colliding-dust-and-the-sparks-of-creation/ status: public title: Adventitious carbon breaks symmetry in oxide contact electrification tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 651 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold PlanS_conform: '1' _id: '21711' abstract: - lang: eng text: 'Background: Low-volume trapping columns are essential for sample enrichment, desalting, and injection profile focusing on nano-LC–MS-based proteomics. They enable higher sample loading, improve chromatographic performance, and protect the analytical column by removing salts and contaminants. Recently, monolithic trap columns with micropillar architecture have emerged as alternatives to conventionally packed traps. This study compares the performance of a packed and a micropillar monolithic trap column for the analysis of tryptic peptides. Methods: A tryptic digest of HeLa cell lysate was analyzed under identical LC–MS conditions using both trap types. Peptides were detected at 214 nm and analyzed by nano-ESI on a Q Exactive Plus Orbitrap. Data were searched against the human UniProt database (February 2023) using FragPipe v20.0, and statistical evaluation of MaxLFQ intensities was performed in Perseus using Welch’s t-test and clustering analysis. Results: Over 2500 proteins were identified with both setups. The packed trap column yielded more total peptides, particularly those with post-translational modifications and higher hydrophilicity, whereas the monolithic column favored peptides of intermediate hydrophobicity. Chromatographic profiles confirmed a slight reduction in the trapping efficiency of hydrophilic peptides by the monolithic trap. Conclusions: Trap column design significantly influences peptide recovery and proteome coverage.' acknowledgement: 'The authors thank Gábor Tóth, Uppsala University, Sweden, and Armel Nicolas, Institute for Science and Technology Austria, for their support. This research was conducted during a student residency in Vienna under the auspices of OeAD. ZI: ICM-2016-03196.' article_number: '10' article_processing_charge: Yes article_type: original author: - first_name: Jadranka full_name: Miletić Vukajlović, Jadranka last_name: Miletić Vukajlović - first_name: Bojana full_name: Ilić, Bojana last_name: Ilić - first_name: Bella full_name: Bruszel, Bella id: 70abbbb3-88ea-11ec-8e0a-e8c939944834 last_name: Bruszel - first_name: Tanja full_name: Panić-Janković, Tanja last_name: Panić-Janković - first_name: Goran full_name: Mitulović, Goran last_name: Mitulović citation: ama: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses. Proteomes. 2026;14(1). doi:10.3390/proteomes14010010 apa: Miletić Vukajlović, J., Ilić, B., Bruszel, B., Panić-Janković, T., & Mitulović, G. (2026). Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses. Proteomes. MDPI. https://doi.org/10.3390/proteomes14010010 chicago: Miletić Vukajlović, Jadranka, Bojana Ilić, Bella Bruszel, Tanja Panić-Janković, and Goran Mitulović. “Comparison of the Trapping Efficiency for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” Proteomes. MDPI, 2026. https://doi.org/10.3390/proteomes14010010. ieee: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, and G. Mitulović, “Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses,” Proteomes, vol. 14, no. 1. MDPI, 2026. ista: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. 2026. Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses. Proteomes. 14(1), 10. mla: Miletić Vukajlović, Jadranka, et al. “Comparison of the Trapping Efficiency for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” Proteomes, vol. 14, no. 1, 10, MDPI, 2026, doi:10.3390/proteomes14010010. short: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, G. Mitulović, Proteomes 14 (2026). date_created: 2026-04-12T22:01:49Z date_published: 2026-03-01T00:00:00Z date_updated: 2026-05-04T10:36:21Z day: '01' ddc: - '540' department: - _id: MassSpec doi: 10.3390/proteomes14010010 external_id: pmid: - '41893725' file: - access_level: open_access checksum: 1e0c66bbf4b6e0be626a8639ea664b63 content_type: application/pdf creator: dernst date_created: 2026-05-04T10:31:35Z date_updated: 2026-05-04T10:31:35Z file_id: '21790' file_name: 2026_Proteomes_Vukajlovic.pdf file_size: 1009723 relation: main_file success: 1 file_date_updated: 2026-05-04T10:31:35Z has_accepted_license: '1' intvolume: ' 14' issue: '1' language: - iso: eng month: '03' oa: 1 oa_version: Published Version pmid: 1 publication: Proteomes publication_identifier: eissn: - 2227-7382 publication_status: published publisher: MDPI quality_controlled: '1' scopus_import: '1' status: public title: Comparison of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar trap columns for proteomics analyses tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 14 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold PlanS_conform: '1' _id: '21746' abstract: - lang: eng text: As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution. acknowledged_ssus: - _id: Bio - _id: LifeSc acknowledgement: 'We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).' article_number: '117227' article_processing_charge: Yes article_type: original author: - first_name: David full_name: Vijatovic, David id: cf391e77-ec3c-11ea-a124-d69323410b58 last_name: Vijatovic - first_name: 'Florina Alexandra ' full_name: 'Toma, Florina Alexandra ' id: 2f73f876-f128-11eb-9611-b96b5a30cb0e last_name: Toma - first_name: Y full_name: Ignatyev, Y last_name: Ignatyev - first_name: Zoe P full_name: Harrington, Zoe P id: a8144562-32c9-11ee-b5ce-d9800628bda2 last_name: Harrington orcid: 0009-0008-0158-4032 - first_name: Christoph M full_name: Sommer, Christoph M id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87 last_name: Sommer orcid: 0000-0003-1216-9105 - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Matthijs Geert full_name: Smits, Matthijs Geert id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0 last_name: Smits - first_name: Marco full_name: Dalla Vecchia, Marco id: 02a7a869-ff06-11ed-a87f-86649d6077e5 last_name: Dalla Vecchia - first_name: Alexandra J. full_name: Trevisan, Alexandra J. last_name: Trevisan - first_name: Phillip full_name: Chapman, Phillip last_name: Chapman - first_name: Mara full_name: Julseth, Mara id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1 last_name: Julseth - first_name: Susan full_name: Brenner-Morton, Susan last_name: Brenner-Morton - first_name: Mariano I. full_name: Gabitto, Mariano I. last_name: Gabitto - first_name: Jeremy S. full_name: Dasen, Jeremy S. last_name: Dasen - first_name: Jay B. full_name: Bikoff, Jay B. last_name: Bikoff - first_name: Lora Beatrice Jaeger full_name: Sweeney, Lora Beatrice Jaeger id: 56BE8254-C4F0-11E9-8E45-0B23E6697425 last_name: Sweeney orcid: 0000-0001-9242-5601 citation: ama: Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 2026;45(4). doi:10.1016/j.celrep.2026.117227 apa: Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2026.117227 chicago: Vijatovic, David, Florina Alexandra Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell Reports. Elsevier, 2026. https://doi.org/10.1016/j.celrep.2026.117227. ieee: D. Vijatovic et al., “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” Cell Reports, vol. 45, no. 4. Elsevier, 2026. ista: Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227. mla: Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell Reports, vol. 45, no. 4, 117227, Elsevier, 2026, doi:10.1016/j.celrep.2026.117227. short: D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026). corr_author: '1' date_created: 2026-04-19T22:07:43Z date_published: 2026-04-28T00:00:00Z date_updated: 2026-05-04T12:27:06Z day: '28' ddc: - '570' department: - _id: LoSw - _id: GradSch - _id: TiVo - _id: Bio - _id: NiBa doi: 10.1016/j.celrep.2026.117227 external_id: pmid: - '41964955 ' file: - access_level: open_access checksum: 0d26cdb5b8d8dec3a911d8261a65cdef content_type: application/pdf creator: dernst date_created: 2026-05-04T12:20:10Z date_updated: 2026-05-04T12:20:10Z file_id: '21795' file_name: 2026_CellReports_Vijatovic.pdf file_size: 14925958 relation: main_file success: 1 file_date_updated: 2026-05-04T12:20:10Z has_accepted_license: '1' intvolume: ' 45' issue: '4' language: - iso: eng month: '04' oa: 1 oa_version: Published Version pmid: 1 project: - _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae grant_number: '101041551' name: Development and Evolution of Tetrapod Motor Circuits - _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e grant_number: F7814 name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity' - _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473 grant_number: CZI01 name: Tools for automation and feedback microscopy - _id: bd73af52-d553-11ed-ba76-912049f0ac7a grant_number: FTI21-D-046 name: Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis publication: Cell Reports publication_identifier: eissn: - 2211-1247 issn: - 2639-1856 publication_status: published publisher: Elsevier quality_controlled: '1' scopus_import: '1' status: public title: Multifold increase in spinal inhibitory cell types with emergence of limb movement tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 45 year: '2026' ... --- OA_type: closed access _id: '21762' abstract: - lang: eng text: Bacteria, like eukaryotes, use conserved cytoskeletal systems for intracellular organization. The plasmid-encoded ParMRC system forms actin-like filaments that segregate low–copy number plasmids. In multicellular cyanobacteria such as Anabaena sp., we found that a chromosomally encoded ParMR system has evolved into a cytoskeletal system named CorMR with a function in cell shape control rather than DNA segregation. Live-cell imaging, in vitro reconstitution, and cryo–electron microscopy revealed that CorM formed dynamically unstable, antiparallel double-stranded filaments that were recruited to the membrane by CorR through an amphipathic helix conserved in multicellular cyanobacteria. CorMR filaments were regulated by MinC, which excluded them from the poles and division plane. Comparative genomics indicated that the repurposing of ParMR and Min systems coevolved with cyanobacterial multicellularity, highlighting the evolutionary plasticity of cytoskeletal systems in bacteria. acknowledged_ssus: - _id: Bio - _id: ScienComp - _id: EM-Fac - _id: LifeSc acknowledgement: "We thank all members of the Loose lab at ISTA for helpful discussions; M. Kojic for critical reading of the manuscript; A. Herrero (Sevilla University) for sharing her extensive BACTH plasmid library and other plasmids, as well as cyanobacterial strains; T. Dagan and F. Nies (both Kiel University) for sharing cyanobacterial strains and plasmids and for valuable discussions; N. Sapay and A. Michon for providing the Amphipaseek code, which enabled us to perform our large-scale amphipathic helix screen of cyanobacterial CorR proteins; V.-V. Hodirnau for support in cryo-ET data collection; and J. Hansen for advice about cryo-EM data processing.\r\nThis work was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF), the Scientific Computing (SciComp), the Electron Microscopy Facility (EMF), and the Lab Support Facility (LSF). This work was funded by the European Union’s Horizon 2020 research and innovation program (Marie Skłodowska-Curie grant 101034413 to B.L.S.); the European Research Council (ERC) of the European Union (grant ActinID 101076260 to F.K.M.S.); the Swiss National Science Foundation (starting grant TMSGI3_226208 to G.L.W.); and the Jean-Jacques et Letitia Lopez-Loreta Foundation (G.L.W.)." article_number: eaea6343 article_processing_charge: No article_type: original author: - first_name: Benjamin L full_name: Springstein, Benjamin L id: b4eb62ef-ac72-11ed-9503-ed3b4d66c083 last_name: Springstein orcid: 0000-0002-3461-5391 - first_name: Manjunath full_name: Javoor, Manjunath id: 305ab18b-dc7d-11ea-9b2f-b58195228ea2 last_name: Javoor orcid: 0000-0003-2311-2112 - first_name: Daniela full_name: Megrian, Daniela last_name: Megrian - first_name: Roman full_name: Hajdu, Roman id: ffab949d-133f-11ed-8f02-94de21ace503 last_name: Hajdu - first_name: Dustin M. full_name: Hanke, Dustin M. last_name: Hanke - first_name: Bettina full_name: Zens, Bettina id: 45FD126C-F248-11E8-B48F-1D18A9856A87 last_name: Zens orcid: 0000-0002-9561-1239 - first_name: Gregor L. full_name: Weiss, Gregor L. last_name: Weiss - first_name: Florian Km full_name: Schur, Florian Km id: 48AD8942-F248-11E8-B48F-1D18A9856A87 last_name: Schur orcid: 0000-0003-4790-8078 - first_name: Martin full_name: Loose, Martin id: 462D4284-F248-11E8-B48F-1D18A9856A87 last_name: Loose orcid: 0000-0001-7309-9724 citation: ama: Springstein BL, Javoor M, Megrian D, et al. Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape. Science. 2026;392(6795). doi:10.1126/science.aea6343 apa: Springstein, B. L., Javoor, M., Megrian, D., Hajdu, R., Hanke, D. M., Zens, B., … Loose, M. (2026). Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape. Science. AAAS. https://doi.org/10.1126/science.aea6343 chicago: Springstein, Benjamin L, Manjunath Javoor, Daniela Megrian, Roman Hajdu, Dustin M. Hanke, Bettina Zens, Gregor L. Weiss, Florian KM Schur, and Martin Loose. “Repurposing of a DNA Segregation Machinery into a Cytoskeletal System Controlling Cell Shape.” Science. AAAS, 2026. https://doi.org/10.1126/science.aea6343. ieee: B. L. Springstein et al., “Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape,” Science, vol. 392, no. 6795. AAAS, 2026. ista: Springstein BL, Javoor M, Megrian D, Hajdu R, Hanke DM, Zens B, Weiss GL, Schur FK, Loose M. 2026. Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape. Science. 392(6795), eaea6343. mla: Springstein, Benjamin L., et al. “Repurposing of a DNA Segregation Machinery into a Cytoskeletal System Controlling Cell Shape.” Science, vol. 392, no. 6795, eaea6343, AAAS, 2026, doi:10.1126/science.aea6343. short: B.L. Springstein, M. Javoor, D. Megrian, R. Hajdu, D.M. Hanke, B. Zens, G.L. Weiss, F.K. Schur, M. Loose, Science 392 (2026). corr_author: '1' date_created: 2026-04-26T22:01:46Z date_published: 2026-04-16T00:00:00Z date_updated: 2026-04-28T13:29:05Z day: '16' department: - _id: MaLo - _id: FlSc - _id: GradSch - _id: EM-Fac doi: 10.1126/science.aea6343 ec_funded: 1 external_id: pmid: - '41990175' intvolume: ' 392' issue: '6795' language: - iso: eng month: '04' oa_version: None pmid: 1 project: - _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c call_identifier: H2020 grant_number: '101034413' name: 'IST-BRIDGE: International postdoctoral program' - _id: bd980d18-d553-11ed-ba76-ceaa645c97eb grant_number: '101076260' name: A molecular atlas of Actin filament IDentities in the cell motility machinery publication: Science publication_identifier: eissn: - 1095-9203 issn: - 0036-8075 publication_status: published publisher: AAAS quality_controlled: '1' scopus_import: '1' status: public title: Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 392 year: '2026' ... --- OA_place: publisher OA_type: hybrid _id: '21767' abstract: - lang: eng text: 'The involvement of non-scientific staff in discussions about animal welfare and scientific quality is essential for biomedical research progress. In this study, we developed a survey to collect the self-perception of animal care staff (ACS) and laboratory technicians about their involvement in scientific planning and conduct. Participants were contacted to complete an anonymous online questionnaire. We obtained 850 responses, mainly from Europe: 564 from ACS and 286 from laboratory technicians. Job satisfaction was assessed as positive by ACS and laboratory technicians despite the low frequency of culture of care activities and mental health meetings. Both groups expressed their desire to be trained in research planning and conduct; however, regular training was not reported. In addition, the inability to act on animal welfare concerns owing to experimental reasons was reported by both groups. Over half of the participants felt valued and appreciated by the lead scientists or animal facility manager; however, it is not clear how they are acknowledged, as their names on the authors list or in the manuscript acknowledgments are barely included. Our results indicated that involvement of ACS and laboratory technicians in planning and conducting studies would improve their understanding of how experiments are done, and therefore communication processes, work satisfaction, animal welfare, and scientific quality. Finally, we provided recommendations to improve the engagement of ACS and laboratory technicians in discussions about animal research planning and conduct.' - lang: fre text: 'La participation de personnel non-scientifique aux discussions sur le bien-être animal et la qualité scientifique est essentielle aux progrès la recherche biomédicale. Dans cette étude, nous avons développé une enquête pour recueillir l''auto-perception du personnel chargé des soins prodigués aux animaux (PCSA) et des techniciens de laboratoire (TL) sur leur implication dans la planification et la conduite scientifiques. Les participants ont été contactés pour remplir un questionnaire anonyme en ligne. Nous avons obtenu 850 réponses, principalement en Europe : 564 provenant de PCSA et 286 de TL. La satisfaction au travail a été évaluée comme positive par le PCSA et les TL malgré la faible fréquence d’activités sur la culture des soins et de réunions concernant la santé mentale. Bien que les deux groupes aient exprimé leur désir d''être formés à la planification et à la conduite de la recherche, aucune formation réelle régulière n''a été signalée. De plus, l''incapacité d''agir sur les préoccupations relatives au bien-être animal pour des raisons expérimentales a été signalée par les deux groupes. Plus de la moitié des participants se sont sentis valorisés et appréciés par les scientifiques principaux ou le gestionnaire de l’installation animale mais on ne sait pas clairement comment ils sont reconnus, car leurs noms sur la liste des auteurs ou dans les remerciements sont à peine inclus dans la documentation. Nos résultats ont indiqué que la participation du PCSA et des TL à la planification et à la conduite des études améliorerait leur compréhension de la façon dont les expériences sont effectuées et, par conséquent, les processus de communication, leur satisfaction au travail ainsi que le bien-être animal et la qualité scientifique. Enfin, nous avons formulé des recommandations pour améliorer la participation du PCSA et des TL aux discussions sur la planification et la conduite de la recherche animale.' - lang: ger text: 'Die Einbeziehung von nichtwissenschaftlichem Personal in Diskussionen über Tierschutz und wissenschaftliche Qualität ist für Fortschritte in biomedizinischer Forschung von entscheidender Bedeutung. In dieser Studie haben wir eine Umfrage entwickelt, um die Selbsteinschätzung von Tierpflegern (ACS) und Labortechnikern (LT) hinsichtlich ihrer Beteiligung an der wissenschaftlichen Planung und Durchführung zu erfassen. Die Teilnehmer wurden gebeten, einen anonymen Online-Fragebogen auszufüllen. Wir erhielten 850 Rückmeldungen, hauptsächlich aus Europa: 564 von ACS und 286 von LT. Die Arbeitszufriedenheit wurde von ACS und LT trotz der geringen Häufigkeit von Pflegeaktivitäten und Treffen zum Thema psychische Gesundheit als positiv bewertet. Beide Gruppen äußerten den Wunsch, in der Forschungsplanung und -durchführung geschult zu werden, doch regelmäßig stattfindende Schulungen wurden nicht berichtet. Außerdem wurde von beiden Gruppen vermeldet, dass sie aus versuchstechnischen Gründen nicht in der Lage waren, auf Tierschutzbedenken zu reagieren. Über die Hälfte der Teilnehmer fühlte sich von den leitenden Wissenschaftlern oder dem Leiter der Tierhaltungseinrichtung geschätzt und anerkannt; es ist jedoch unklar, inwiefern sie wirklich gewürdigt werden, da ihre Namen kaum in der Autorenliste oder in den Danksagungen des Manuskripts aufgeführt sind. Unsere Ergebnisse deuteten darauf hin, dass die Einbeziehung von ACS und LT in die Planung und Durchführung von Studien ihr Verständnis für die Durchführung von Experimenten verbessern würde – und damit auch Kommunikationsprozesse, Arbeitszufriedenheit, Tierwohl und wissenschaftliche Qualität. Abschließend gaben wir Empfehlungen zur Verbesserung der Einbeziehung von ACS und LT in Diskussionen über die Planung und Durchführung von Tierversuchen.' - lang: spa text: 'La participación del personal no científico en los debates sobre el bienestar animal y la calidad científica es fundamental para el avance de la investigación biomédica. En este estudio, desarrollamos una encuesta para recoger la autopercepción del personal encargado del cuidado de los animales (ACS) y de los técnicos de laboratorio (LT) sobre su implicación en la planificación y la realización científicas. Se contactó con los participantes para que cumplimentaran un cuestionario anónimo en línea. Obtuvimos 850 respuestas, principalmente de Europa: 564 de ACS y 286 de LT. La satisfacción laboral fue evaluada como positiva por ACS y técnicos de laboratorio a pesar de la baja frecuencia de actividades de cultura del cuidado y reuniones sobre bienestar mental. Ambos grupos expresaron su deseo de recibir formación en planificación y realización de investigaciones, sin embargo, no se informó sobre una formación regular. Asimismo, ambos grupos señalaron la incapacidad de actuar ante las preocupaciones sobre el bienestar animal por motivos experimentales. Más de la mitad de los participantes se sintieron valorados y apreciados por los científicos principales o el responsable de las instalaciones de animales; sin embargo, no está claro cómo se les reconoce, ya que apenas se incluyen sus nombres en la lista de autores o en los agradecimientos del manuscrito. Nuestros resultados indicaron que la participación de los ACS y los LT en la planificación y realización de los estudios mejoraría su comprensión de cómo se hacen los experimentos y, por tanto, los procesos de comunicación, la satisfacción laboral, el bienestar animal y la calidad científica. Finalmente, proporcionamos recomendaciones para mejorar el compromiso de la AEC y la LT en los debates sobre la planificación y la realización de investigaciones con animales.' acknowledgement: "We deeply acknowledge all the animal care staff and laboratory technicians who participated in this study! We acknowledge Working Groups 1 and 4 from COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”), CA21139, supported by COST (European Cooperation in Science and Technology) for their feedback and support. We also acknowledge Aoife Milford for her comments and contributions to the final draft of the manuscript.\r\nThis publication was based on work from the COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”), CA21139, supported by COST (European Cooperation in Science and Technology)." article_processing_charge: Yes (in subscription journal) article_type: original author: - first_name: Fernando full_name: Gonzalez-Uarquin, Fernando last_name: Gonzalez-Uarquin - first_name: Paulin full_name: Jirkof, Paulin last_name: Jirkof - first_name: Bettina full_name: Bert, Bettina last_name: Bert - first_name: Penny full_name: Hawkins, Penny last_name: Hawkins - first_name: Ljupco full_name: Angelovski, Ljupco last_name: Angelovski - first_name: Jan full_name: Baumgart, Jan last_name: Baumgart - first_name: Nadine full_name: Baumgart, Nadine last_name: Baumgart - first_name: Özge S. full_name: Cevik, Özge S. last_name: Cevik - first_name: Nuno H. full_name: Franco, Nuno H. last_name: Franco - first_name: Erdal full_name: Horata, Erdal last_name: Horata - first_name: Rohish full_name: Kaura, Rohish last_name: Kaura - first_name: Winfried full_name: Neuhaus, Winfried last_name: Neuhaus - first_name: Brigida full_name: Riso, Brigida last_name: Riso - first_name: Adrian J. full_name: Smith, Adrian J. last_name: Smith - first_name: Athanassia full_name: Sotiropoulos, Athanassia last_name: Sotiropoulos - first_name: Augusto full_name: Vitale, Augusto last_name: Vitale - first_name: Sophie full_name: Schober, Sophie id: 80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8 last_name: Schober citation: ama: 'Gonzalez-Uarquin F, Jirkof P, Bert B, et al. Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science. Laboratory Animals. 2026. doi:10.1177/00236772251400976' apa: 'Gonzalez-Uarquin, F., Jirkof, P., Bert, B., Hawkins, P., Angelovski, L., Baumgart, J., … Schober, S. (2026). Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science. Laboratory Animals. SAGE Publications. https://doi.org/10.1177/00236772251400976' chicago: 'Gonzalez-Uarquin, Fernando, Paulin Jirkof, Bettina Bert, Penny Hawkins, Ljupco Angelovski, Jan Baumgart, Nadine Baumgart, et al. “Building Bridges: Involvement of Animal Care Staff and Laboratory Technicians in Experimental Planning and Conduct of Animal Studies for Better Job Satisfaction and Science.” Laboratory Animals. SAGE Publications, 2026. https://doi.org/10.1177/00236772251400976.' ieee: 'F. Gonzalez-Uarquin et al., “Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science,” Laboratory Animals. SAGE Publications, 2026.' ista: 'Gonzalez-Uarquin F, Jirkof P, Bert B, Hawkins P, Angelovski L, Baumgart J, Baumgart N, Cevik ÖS, Franco NH, Horata E, Kaura R, Neuhaus W, Riso B, Smith AJ, Sotiropoulos A, Vitale A, Schober S. 2026. Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science. Laboratory Animals.' mla: 'Gonzalez-Uarquin, Fernando, et al. “Building Bridges: Involvement of Animal Care Staff and Laboratory Technicians in Experimental Planning and Conduct of Animal Studies for Better Job Satisfaction and Science.” Laboratory Animals, SAGE Publications, 2026, doi:10.1177/00236772251400976.' short: F. Gonzalez-Uarquin, P. Jirkof, B. Bert, P. Hawkins, L. Angelovski, J. Baumgart, N. Baumgart, Ö.S. Cevik, N.H. Franco, E. Horata, R. Kaura, W. Neuhaus, B. Riso, A.J. Smith, A. Sotiropoulos, A. Vitale, S. Schober, Laboratory Animals (2026). date_created: 2026-04-26T22:01:47Z date_published: 2026-04-14T00:00:00Z date_updated: 2026-06-18T08:33:36Z day: '14' ddc: - '570' department: - _id: PreCl doi: 10.1177/00236772251400976 language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1177/00236772251400976 month: '04' oa: 1 oa_version: Published Version publication: Laboratory Animals publication_identifier: eissn: - 1758-1117 issn: - 0023-6772 publication_status: epub_ahead publisher: SAGE Publications quality_controlled: '1' scopus_import: '1' status: public title: 'Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science' type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '21779' abstract: - lang: eng text: Acidomycin is an anti-mycobacterial antibiotic with a unique mode of action, targeting the biotin biosynthesis pathway. Despite being highly active against mycobacteria in vitro, its development as an anti-tubercular agent has been hindered due to suboptimal pharmacokinetics. Engineering of the acidomycin biosynthesis may yield new analogues with improved pharmacological properties. Here, we describe the identification of the acidomycin biosynthetic gene cluster (BGC) in a Streptomyces bacterium isolated from the rhizosphere of Edelweiss. Notably, the acidomycin BGC is located in proximity to the genes for the biosynthesis of stravidins, secondary metabolites targeting a different enzyme in the biotin biosynthesis pathway, and two genes for streptavidins, proteins that strongly bind and sequester biotin. The identity of the acidomycin BGC was confirmed via both gene knock-out and heterologous expression, which suggested that the fatty acid required for the formation of acidomycin's acyl chain is most likely scavenged from the biotin biosynthesis pathway. CRISPR/Cas9-assisted knock-out of the cytochrome P450-encoding gene in the acidomycin BGC resulted in a significant decrease in its yield but did not abrogate the biosynthesis completely. acknowledgement: This work was supported by the University of Vienna. The authors thank Anna Fabisikova from the Mass Spectrometry Centre and the team of the NMR Centre (both of the Faculty of Chemistry, University of Vienna and members of the Vienna Life Science Instruments) for assistance with data acquisition. Open Access funding provided by Universitat Wien. This work was supported by Universität Wien. article_number: e70357 article_processing_charge: Yes article_type: original author: - first_name: Anna full_name: Vignolle, Anna last_name: Vignolle - first_name: Martin full_name: Zehl, Martin id: 8e016d5b-5d77-11f0-86d2-96cdb3922a55 last_name: Zehl orcid: 0000-0001-9685-0373 - first_name: Jaime Felipe Guerrero full_name: Garzón, Jaime Felipe Guerrero last_name: Garzón - first_name: Olha full_name: Schneider, Olha last_name: Schneider - first_name: Johannes full_name: Gafriller, Johannes last_name: Gafriller - first_name: Ulrike full_name: Grienke, Ulrike last_name: Grienke - first_name: Rasmus H. full_name: Kirkegaard, Rasmus H. last_name: Kirkegaard - first_name: Sergey B. full_name: Zotchev, Sergey B. last_name: Zotchev citation: ama: Vignolle A, Zehl M, Garzón JFG, et al. Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial Biotechnology. 2026;19(4). doi:10.1111/1751-7915.70357 apa: Vignolle, A., Zehl, M., Garzón, J. F. G., Schneider, O., Gafriller, J., Grienke, U., … Zotchev, S. B. (2026). Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial Biotechnology. Wiley. https://doi.org/10.1111/1751-7915.70357 chicago: Vignolle, Anna, Martin Zehl, Jaime Felipe Guerrero Garzón, Olha Schneider, Johannes Gafriller, Ulrike Grienke, Rasmus H. Kirkegaard, and Sergey B. Zotchev. “Identification and Characterisation of the Gene Cluster Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” Microbial Biotechnology. Wiley, 2026. https://doi.org/10.1111/1751-7915.70357. ieee: A. Vignolle et al., “Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin,” Microbial Biotechnology, vol. 19, no. 4. Wiley, 2026. ista: Vignolle A, Zehl M, Garzón JFG, Schneider O, Gafriller J, Grienke U, Kirkegaard RH, Zotchev SB. 2026. Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial Biotechnology. 19(4), e70357. mla: Vignolle, Anna, et al. “Identification and Characterisation of the Gene Cluster Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” Microbial Biotechnology, vol. 19, no. 4, e70357, Wiley, 2026, doi:10.1111/1751-7915.70357. short: A. Vignolle, M. Zehl, J.F.G. Garzón, O. Schneider, J. Gafriller, U. Grienke, R.H. Kirkegaard, S.B. Zotchev, Microbial Biotechnology 19 (2026). date_created: 2026-05-03T22:01:37Z date_published: 2026-04-01T00:00:00Z date_updated: 2026-05-07T08:22:41Z day: '01' ddc: - '570' department: - _id: MassSpec doi: 10.1111/1751-7915.70357 external_id: pmid: - '42036976' file: - access_level: open_access checksum: 8c8aa660cef5394167e06f187adbabf0 content_type: application/pdf creator: dernst date_created: 2026-05-07T08:21:06Z date_updated: 2026-05-07T08:21:06Z file_id: '21835' file_name: 2026_MicrobialBiotechnology_Vignolle.pdf file_size: 575492 relation: main_file success: 1 file_date_updated: 2026-05-07T08:21:06Z has_accepted_license: '1' intvolume: ' 19' issue: '4' language: - iso: eng month: '04' oa: 1 oa_version: Published Version pmid: 1 publication: Microbial Biotechnology publication_identifier: eissn: - 1751-7915 publication_status: published publisher: Wiley quality_controlled: '1' scopus_import: '1' status: public title: Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 19 year: '2026' ... --- OA_place: repository OA_type: green _id: '21848' abstract: - lang: eng text: 'Despite the success of mRNA therapeutics, challenges remain in optimizing immune responses and minimizing side effects. Cell-specific antigen delivery may help reduce required doses and improve vaccine efficacy. In this study, we report on a targeted delivery system for mRNA to a specific subset of skin-resident antigen-presenting cells: Langerhans cells. By functionalizing lipid nanoparticles with a langerin-specific glycomimetic ligand, we achieve selective mRNA delivery to both murine and human primary Langerhans cells with minimal off-target uptake, at the same time resulting in significantly increased mRNA translation. This targeted mRNA delivery not only enhances antigen presentation and T-cell responses but also enables dose-sparing and superior antitumor immunity compared with conventional immunization in a B16-OVA tumor model. Importantly, our platform’s high compatibility with various lipid nanoparticle formulations offers a flexible and precise tool for skin-directed mRNA delivery.' acknowledged_ssus: - _id: PreCl acknowledgement: We thank Mareike Rentzsch for her intellectual contributions during the course of our discussions. We thank Michael Schunn from the Preclinical Facility of the Institute of Science and Technology Austria for his continuous technical support. Guarantor of the work is FS. This project was supported by “Seedfinancing” (P2282679) of the Austrian Federal Ministry of Digital and Economic Affairs and the Ministry of Climate Action and Energy, handled by the Austrian Wirtschaftsservice, as well as by... article_processing_charge: No article_type: original author: - first_name: Klara full_name: Klein, Klara last_name: Klein - first_name: Litty full_name: Johnson, Litty last_name: Johnson - first_name: Ramona full_name: Rîca, Ramona last_name: Rîca - first_name: Mirza full_name: Sarcevic, Mirza last_name: Sarcevic - first_name: Gabriele full_name: Carta, Gabriele last_name: Carta - first_name: Saskia full_name: Seiser, Saskia last_name: Seiser - first_name: Adelheid full_name: Elbe-Bürger, Adelheid last_name: Elbe-Bürger - first_name: Freyja full_name: Langer, Freyja id: 3C1BE782-F248-11E8-B48F-1D18A9856A87 last_name: Langer - first_name: Nowras full_name: Rahhal, Nowras last_name: Rahhal - first_name: Christoph full_name: Rademacher, Christoph last_name: Rademacher - first_name: Robert full_name: Wawrzinek, Robert last_name: Wawrzinek - first_name: Federica full_name: Quattrone, Federica last_name: Quattrone - first_name: Florian full_name: Sparber, Florian last_name: Sparber citation: ama: 'Klein K, Johnson L, Rîca R, et al. Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity. Journal of Investigative Dermatology. doi:10.1016/j.jid.2026.03.026' apa: 'Klein, K., Johnson, L., Rîca, R., Sarcevic, M., Carta, G., Seiser, S., … Sparber, F. (n.d.). Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity. Journal of Investigative Dermatology. Elsevier. https://doi.org/10.1016/j.jid.2026.03.026' chicago: 'Klein, Klara, Litty Johnson, Ramona Rîca, Mirza Sarcevic, Gabriele Carta, Saskia Seiser, Adelheid Elbe-Bürger, et al. “Langerhans Cell–Targeted MRNA Delivery: A Strategy for Dose-Sparing and Enhanced Antitumor Immunity.” Journal of Investigative Dermatology. Elsevier, n.d. https://doi.org/10.1016/j.jid.2026.03.026.' ieee: 'K. Klein et al., “Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity,” Journal of Investigative Dermatology. Elsevier.' ista: 'Klein K, Johnson L, Rîca R, Sarcevic M, Carta G, Seiser S, Elbe-Bürger A, Langer F, Rahhal N, Rademacher C, Wawrzinek R, Quattrone F, Sparber F. Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity. Journal of Investigative Dermatology.' mla: 'Klein, Klara, et al. “Langerhans Cell–Targeted MRNA Delivery: A Strategy for Dose-Sparing and Enhanced Antitumor Immunity.” Journal of Investigative Dermatology, Elsevier, doi:10.1016/j.jid.2026.03.026.' short: K. Klein, L. Johnson, R. Rîca, M. Sarcevic, G. Carta, S. Seiser, A. Elbe-Bürger, F. Langer, N. Rahhal, C. Rademacher, R. Wawrzinek, F. Quattrone, F. Sparber, Journal of Investigative Dermatology (n.d.). date_created: 2026-05-10T22:02:16Z date_published: 2026-04-07T00:00:00Z date_updated: 2026-05-11T06:07:32Z day: '07' department: - _id: PreCl doi: 10.1016/j.jid.2026.03.026 language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1101/2025.06.25.661517 month: '04' oa: 1 oa_version: Preprint publication: Journal of Investigative Dermatology publication_identifier: eissn: - 1523-1747 issn: - 0022-202X publication_status: inpress publisher: Elsevier scopus_import: '1' status: public title: 'Langerhans cell–targeted mRNA delivery: A strategy for dose-sparing and enhanced antitumor immunity' type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '21860' abstract: - lang: eng text: Glutamate excitotoxicity is a cell death mechanism triggered by accumulation of glutamate in the extracellular space. The α-ketoglutarate dehydrogenase complex (αKGDHC), an enzyme of the tricarboxylic acid cycle, represents a branching point controlling glutamate formation and its consumption as a fuel. Hence, modulation of the activity of αKGDHC might alter the amount of glutamate available for excitotoxic effects. To address this hypothesis, hippocampal neurons in primary co-culture with glial cells were exposed to zero-Mg2 buffer to elicit excitotoxicity through N-methyl-D-aspartic acid (NMDA) receptor disinhibition. Pretreatment of the cultures with succinyl phosphonate, to inhibit αKGDHC, enhanced excitotoxity, whereas promotion of αKGDHC activity by pretreatment with thiamine caused an opposite action. Moreover, NMDA receptor currents – but not those mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors – were potentiated in neurons with impaired αKGDHC activity and diminished in neurons with boosted αKGDHC activity. The sensitization of NMDA receptors involved mGluR1 activation and was accompanied by enhanced neuronal discharge activity, elevated basal cytosolic Ca2+ levels, and augmented Ca2+ responses evoked by glutamate application. These results suggest that mGluR1-mediated potentiation of NMDA receptors contributes to a mechanism by which inhibition of αKGDHC might exacerbate glutamate excitotoxicity. acknowledgement: The technical assistance by Tanja Wagner and Elena Lilliu is gratefully acknowledged. This research was funded in whole or in part by the Austrian Science Fund (FWF) (P36145 to H.K., PAT8605623 to M.H. and P33799 to A.V.K.]. Open Access funding provided by Medical University of Vienna and the Austrian Science Fund (FWF). Deposited in PMC for immediate release. article_number: jcs264420 article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Vanessa full_name: Goeschl, Vanessa last_name: Goeschl - first_name: Matej full_name: Hotka, Matej last_name: Hotka - first_name: Bernhard full_name: Hochreiter, Bernhard id: e6cab3de-17f6-11ed-9210-c1e42e045e9d last_name: Hochreiter - first_name: Karlheinz full_name: Hilber, Karlheinz last_name: Hilber - first_name: Stefan full_name: Boehm, Stefan last_name: Boehm - first_name: Andrey V. full_name: Kozlov, Andrey V. last_name: Kozlov - first_name: Helmut full_name: Kubista, Helmut last_name: Kubista citation: ama: Goeschl V, Hotka M, Hochreiter B, et al. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. Journal of Cell Science. 2026;139(8). doi:10.1242/jcs.264420 apa: Goeschl, V., Hotka, M., Hochreiter, B., Hilber, K., Boehm, S., Kozlov, A. V., & Kubista, H. (2026). α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.264420 chicago: Goeschl, Vanessa, Matej Hotka, Bernhard Hochreiter, Karlheinz Hilber, Stefan Boehm, Andrey V. Kozlov, and Helmut Kubista. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary Cultures.” Journal of Cell Science. The Company of Biologists, 2026. https://doi.org/10.1242/jcs.264420. ieee: V. Goeschl et al., “α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures,” Journal of Cell Science, vol. 139, no. 8. The Company of Biologists, 2026. ista: Goeschl V, Hotka M, Hochreiter B, Hilber K, Boehm S, Kozlov AV, Kubista H. 2026. α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures. Journal of Cell Science. 139(8), jcs264420. mla: Goeschl, Vanessa, et al. “α-Ketoglutarate Dehydrogenase Complex Activity Modulates Glutamate Excitotoxicity via Metabotropic Regulation of NMDA Receptors in Primary Cultures.” Journal of Cell Science, vol. 139, no. 8, jcs264420, The Company of Biologists, 2026, doi:10.1242/jcs.264420. short: V. Goeschl, M. Hotka, B. Hochreiter, K. Hilber, S. Boehm, A.V. Kozlov, H. Kubista, Journal of Cell Science 139 (2026). date_created: 2026-05-11T10:52:27Z date_published: 2026-04-27T00:00:00Z date_updated: 2026-05-12T06:40:18Z day: '27' ddc: - '570' department: - _id: Bio doi: 10.1242/jcs.264420 external_id: pmid: - '41834724' file: - access_level: open_access checksum: 8db35c97588c2f6ef88c7e8d5924cf8c content_type: application/pdf creator: dernst date_created: 2026-05-12T06:27:54Z date_updated: 2026-05-12T06:27:54Z file_id: '21861' file_name: 2026_JourCellScience_Goeschl.pdf file_size: 1957057 relation: main_file success: 1 file_date_updated: 2026-05-12T06:27:54Z has_accepted_license: '1' intvolume: ' 139' issue: '8' language: - iso: eng month: '04' oa: 1 oa_version: Published Version pmid: 1 publication: Journal of Cell Science publication_identifier: eissn: - 1477-9137 issn: - 0021-9533 publication_status: published publisher: The Company of Biologists quality_controlled: '1' scopus_import: '1' status: public title: α-ketoglutarate dehydrogenase complex activity modulates glutamate excitotoxicity via metabotropic regulation of NMDA receptors in primary cultures tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 139 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold PlanS_conform: '1' _id: '21953' abstract: - lang: eng text: Several Streptomyces strains were isolated from freshwater sediments collected in the Laxenburg ponds (Lower Austria). Genome sequencing and bioinformatics analyses revealed biosynthetic gene clusters (BGCs) that may specify production of chemically diverse secondary metabolites. Various culture conditions were employed to induce metabolite production, and subsequent LC-MS analyses facilitated the identification of the produced compounds and their correlation with the corresponding BGCs. These analyses of sediment-derived Streptomyces spp. highlight their extensive biosynthetic potential, revealing a diverse range of bioactive secondary metabolites, including siderophores, antibiotics, and other compounds with potential therapeutic applications. Genomes of two Streptomyces isolates, one of them representing a potentially new species, harbored several uncharacterized BGCs that may specify biosynthesis of novel secondary metabolites. Although targeted overexpression of pathway-specific regulators from these BGCs did not yield additional metabolites, whereas knockout experiments led to metabolic changes, presumably reflecting regulatory or compensatory interactions between multiple biosynthetic pathways. Continued exploration of these strains and their BGCs may lead to the discovery of new bioactive molecules with pharmaceutical and biotechnological applications. acknowledgement: "The computational results of this work have been achieved using the Life Science Compute Cluster (LiSC) of the University of Vienna. We additionally thank Julia Ramesmayer for assistance during DNA extraction and sample preparation for long-read sequencing. Support from the Mass Spectrometry Centre of the Faculty of Chemistry, University of Vienna, is thankfully acknowledged.\r\nThe author(s) declared that financial support was received for this work and/or its publication. This work was supported by the University of Vienna via the Research Platform Secondary Metabolomes of Bacterial Communities (MetaBac). Open access funding provided by University of Vienna. " article_number: '1793713' article_processing_charge: Yes article_type: original author: - first_name: Inmaculada full_name: Tocino-Márquez, Inmaculada last_name: Tocino-Márquez - first_name: Martin full_name: Zehl, Martin id: 8e016d5b-5d77-11f0-86d2-96cdb3922a55 last_name: Zehl orcid: 0000-0001-9685-0373 - first_name: Jovana full_name: Batajic, Jovana last_name: Batajic - first_name: Joana full_name: Séneca, Joana last_name: Séneca - first_name: Petra full_name: Pjevac, Petra last_name: Pjevac - first_name: José full_name: Murillo-Alba, José last_name: Murillo-Alba - first_name: Jesús full_name: Martín, Jesús last_name: Martín - first_name: Olga N. full_name: Sekurova, Olga N. last_name: Sekurova - first_name: Sergey B. full_name: Zotchev, Sergey B. last_name: Zotchev citation: ama: Tocino-Márquez I, Zehl M, Batajic J, et al. Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology. 2026;17. doi:10.3389/fmicb.2026.1793713 apa: Tocino-Márquez, I., Zehl, M., Batajic, J., Séneca, J., Pjevac, P., Murillo-Alba, J., … Zotchev, S. B. (2026). Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology. Frontiers Media. https://doi.org/10.3389/fmicb.2026.1793713 chicago: Tocino-Márquez, Inmaculada, Martin Zehl, Jovana Batajic, Joana Séneca, Petra Pjevac, José Murillo-Alba, Jesús Martín, Olga N. Sekurova, and Sergey B. Zotchev. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp. from Freshwater Sediments.” Frontiers in Microbiology. Frontiers Media, 2026. https://doi.org/10.3389/fmicb.2026.1793713. ieee: I. Tocino-Márquez et al., “Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments,” Frontiers in Microbiology, vol. 17. Frontiers Media, 2026. ista: Tocino-Márquez I, Zehl M, Batajic J, Séneca J, Pjevac P, Murillo-Alba J, Martín J, Sekurova ON, Zotchev SB. 2026. Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology. 17, 1793713. mla: Tocino-Márquez, Inmaculada, et al. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp. from Freshwater Sediments.” Frontiers in Microbiology, vol. 17, 1793713, Frontiers Media, 2026, doi:10.3389/fmicb.2026.1793713. short: I. Tocino-Márquez, M. Zehl, J. Batajic, J. Séneca, P. Pjevac, J. Murillo-Alba, J. Martín, O.N. Sekurova, S.B. Zotchev, Frontiers in Microbiology 17 (2026). date_created: 2026-06-08T08:34:10Z date_published: 2026-04-20T00:00:00Z date_updated: 2026-06-10T07:49:04Z day: '20' ddc: - '572' department: - _id: MassSpec doi: 10.3389/fmicb.2026.1793713 external_id: pmid: - '42088272' file: - access_level: open_access checksum: 31fb6b98c8a6d4007cb21808c6d2d9e3 content_type: application/pdf creator: dernst date_created: 2026-06-10T07:46:30Z date_updated: 2026-06-10T07:46:30Z file_id: '21989' file_name: 2026_FrontiersMicrobiology_TocinoMarquez.pdf file_size: 3582644 relation: main_file success: 1 file_date_updated: 2026-06-10T07:46:30Z has_accepted_license: '1' intvolume: ' 17' language: - iso: eng month: '04' oa: 1 oa_version: Published Version pmid: 1 publication: Frontiers in Microbiology publication_identifier: issn: - 1664-302X publication_status: published publisher: Frontiers Media quality_controlled: '1' status: public title: Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 17 year: '2026' ... --- OA_place: repository OA_type: green _id: '21963' abstract: - lang: eng text: The cerebral cortex consists of immense numbers of neuronal and glial cell-types derived from radial glial progenitor (RGP) cells. How RGPs generate appropriate quantities of distinct cortical cell-types to safeguard a brain of correct size, is not well understood. However, genetic aberration in human, including mutations in PTEN, lead to cortical malformation such as macrocephaly, albeit with unknown etiology. Here we utilized Mosaic Analysis with Double Markers (MADM)-based clonal analysis and single cell phenotyping to decipher the role of Pten in neurogenic and gliogenic RGP lineage progression during cortical ontogeny. While neurogenic RGP lineage progression and projection neuron production was moderately altered in the absence of Pten, cortical astrocyte production was drastically increased. Through genetic epistasis experiments we show that the loss of Pten uncouples astrocyte generation from essential growth factor signaling hubs, funneling into MAPK. Collectively, our results suggest that Pten regulates RGP lineage progression with distinct sequential functions in cortical projection neurogenesis and astrocyte production to ensure the emergence of a correctly-sized cerebral cortex. acknowledged_ssus: - _id: Bio - _id: LifeSc - _id: PreCl acknowledgement: "We thank Kay-Uwe Wagner (Wayne State University) for generously sharing Jak1/2–flox mouse lines; A.\r\nSommer (VBCF GmbH, NGS Unit) for technical support; N. Kim, V. Mick, S. Schnabl, S. Gobeil, and L.\r\nAndersen for technical assistance; all members of the Hippenmeyer lab for discussion and B. Novitch for\r\ncomments on earlier versions of the manuscript. This research was supported by the Scientific Service Units\r\n(SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). O.A.M received support from the Austrian Academy of Sciences\r\nÖAW (DOC 186584), and N.A. from FWF Elise Richter Program (Grant V1041T). This work was also\r\nsupported by IST Austria institutional funds; FWF SFB F78 (Neuro Stem Modulation) to S.H., and the\r\nEuropean Research Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogramme (grant agreement No 725780 LinPro) to S.H." article_processing_charge: No author: - first_name: Osvaldo full_name: Miranda, Osvaldo id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425 last_name: Miranda orcid: 0000-0001-6618-6889 - first_name: Ximena full_name: Contreras, Ximena id: 475990FE-F248-11E8-B48F-1D18A9856A87 last_name: Contreras - first_name: Florian full_name: Pauler, Florian id: 48EA0138-F248-11E8-B48F-1D18A9856A87 last_name: Pauler orcid: 0000-0002-7462-0048 - first_name: Amarbayasgalan full_name: Davaatseren, Amarbayasgalan id: 70ADC922-B424-11E9-99E3-BA18E6697425 last_name: Davaatseren - first_name: Nicole full_name: Amberg, Nicole id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87 last_name: Amberg orcid: 0000-0002-3183-8207 - first_name: Carmen full_name: Streicher, Carmen id: 36BCB99C-F248-11E8-B48F-1D18A9856A87 last_name: Streicher - first_name: Ana full_name: Villalba Requena, Ana id: 68cb85a0-39f7-11eb-9559-9aaab4f6a247 last_name: Villalba Requena orcid: 0000-0002-5615-5277 - first_name: Anna-Magdalena full_name: Heger, Anna-Magdalena id: 4B76FFD2-F248-11E8-B48F-1D18A9856A87 last_name: Heger - first_name: Corentine full_name: Marie, Corentine last_name: Marie - first_name: Bassem A. full_name: Hassan, Bassem A. last_name: Hassan - first_name: Thomas full_name: Rülicke, Thomas last_name: Rülicke - first_name: Simon full_name: Hippenmeyer, Simon id: 37B36620-F248-11E8-B48F-1D18A9856A87 last_name: Hippenmeyer orcid: 0000-0003-2279-1061 citation: ama: Miranda O, Contreras X, Pauler F, et al. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv. doi:10.64898/2026.05.01.722191 apa: Miranda, O., Contreras, X., Pauler, F., Davaatseren, A., Amberg, N., Streicher, C., … Hippenmeyer, S. (n.d.). Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv. https://doi.org/10.64898/2026.05.01.722191 chicago: Miranda, Osvaldo, Ximena Contreras, Florian Pauler, Amarbayasgalan Davaatseren, Nicole Amberg, Carmen Streicher, Ana Villalba Requena, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” BioRxiv, n.d. https://doi.org/10.64898/2026.05.01.722191. ieee: O. Miranda et al., “Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production,” bioRxiv. . ista: Miranda O, Contreras X, Pauler F, Davaatseren A, Amberg N, Streicher C, Villalba Requena A, Heger A-M, Marie C, Hassan BA, Rülicke T, Hippenmeyer S. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv, 10.64898/2026.05.01.722191. mla: Miranda, Osvaldo, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” BioRxiv, doi:10.64898/2026.05.01.722191. short: O. Miranda, X. Contreras, F. Pauler, A. Davaatseren, N. Amberg, C. Streicher, A. Villalba Requena, A.-M. Heger, C. Marie, B.A. Hassan, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.). corr_author: '1' date_created: 2026-06-09T08:08:53Z date_published: 2026-05-05T00:00:00Z date_updated: 2026-06-16T08:57:20Z day: '05' ddc: - '570' department: - _id: SiHi - _id: PreCl - _id: GradSch doi: 10.64898/2026.05.01.722191 ec_funded: 1 has_accepted_license: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.64898/2026.05.01.722191 month: '05' oa: 1 oa_version: Preprint project: - _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E grant_number: F7805 name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression - _id: 260018B0-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '725780' name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development publication: bioRxiv publication_status: submitted status: public title: Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production tmp: image: /images/cc_by_nc.png legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) short: CC BY-NC (4.0) type: preprint user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold PlanS_conform: '1' _id: '22229' abstract: - lang: eng text: Hippocampal CA3 pyramidal neurons (PNs) form the largest autoassociative network in the mammalian brain. Whether CA3–CA3 recurrent connectivity is genetically preconfigured or environmentally shaped during ongoing memory storage is currently unknown. To address this question, we performed multicellular patch-clamp-based circuit mapping of up to eight CA3 PNs in the mouse hippocampus at multiple postnatal time points (P7–8, P18–25, and P45–50). Here, we show that the hippocampal CA3 network undergoes a developmental transformation from local, dense, and random connectivity to a distributed, sparse, and structured configuration. Thus, sparse and structured connectivity may emerge via experience-dependent mechanisms. In parallel, the strength of single synapses is downregulated; single synaptic events are sufficient to trigger postsynaptic spiking early in development, whereas spatial summation of several inputs is required at later time points. Biologically inspired models of memory storage by Hebbian synaptic plasticity and retrieval via pattern completion suggest that developmental changes improve specific aspects of memory storage and retrieval. Our results imply a developmental transformation of the neuronal code and the memory functions in the hippocampal CA3 network. acknowledged_ssus: - _id: PreCl - _id: Bio - _id: M-Shop - _id: ScienComp acknowledgement: 'We thank Jose Guzman, Simon Hippenmeyer, and Tim Vogels for critically reading the manuscript, Jozsef Csicsvari for useful discussions, Florian Marr for technical assistance, and Eleftheria Kralli-Beller for manuscript editing. This research was supported by the Scientific Services Units (SSUs) of ISTA: the preclinical facility (PCF) provided housing and breeding of the animals, the imaging and optics facility (IOF) offered technical training and state of the art equipment, the Miba machine shop contributed to the construction and maintenance of multicellular recording setups, and the scientific computing unit helped with the large-scale simulations. The project received funding from the European Union’s Horizon 2020 research and innovation programme (ERC Advanced Grants No 692692 GIANTSYN and 101199096 CA3-SYNGRAM to P.J.; Marie Skłodowska-Curie Grant 754411 to V.V.B.; Marie Skłodowska-Curie Grant 101026635 to J.F.W.), the Fond zur Förderung der Wissenschaftlichen Forschung (P 36232-B, PAT4178023, and 10.55776/CoE16 to P.J.), and the Nomis Foundation (fellowship to A.N.-O.). V.V.B. received funding from a CONACyT fellowship (289638).' article_number: '5540' article_processing_charge: Yes article_type: original author: - first_name: Victor M full_name: Vargas Barroso, Victor M id: 2F55A9DE-F248-11E8-B48F-1D18A9856A87 last_name: Vargas Barroso - first_name: Jake full_name: Watson, Jake id: 63836096-4690-11EA-BD4E-32803DDC885E last_name: Watson orcid: 0000-0002-8698-3823 - first_name: Andrea C full_name: Navas Olivé, Andrea C id: 739d26c9-52e8-11ee-8d72-f14d3893b4ce last_name: Navas Olivé orcid: 0000-0002-9280-8597 - first_name: Alois full_name: Schlögl, Alois id: 45BF87EE-F248-11E8-B48F-1D18A9856A87 last_name: Schlögl orcid: 0000-0002-5621-8100 - first_name: Peter M full_name: Jonas, Peter M id: 353C1B58-F248-11E8-B48F-1D18A9856A87 last_name: Jonas orcid: 0000-0001-5001-4804 citation: ama: Vargas Barroso VM, Watson J, Navas Olivé AC, Schlögl A, Jonas PM. Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. Nature Communications. 2026;17. doi:10.1038/s41467-026-71914-x apa: Vargas Barroso, V. M., Watson, J., Navas Olivé, A. C., Schlögl, A., & Jonas, P. M. (2026). Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-026-71914-x chicago: Vargas Barroso, Victor M, Jake Watson, Andrea C Navas Olivé, Alois Schlögl, and Peter M Jonas. “Developmental Emergence of Sparse and Structured Synaptic Connectivity in the Hippocampal CA3 Memory Circuit.” Nature Communications. Springer Nature, 2026. https://doi.org/10.1038/s41467-026-71914-x. ieee: V. M. Vargas Barroso, J. Watson, A. C. Navas Olivé, A. Schlögl, and P. M. Jonas, “Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit,” Nature Communications, vol. 17. Springer Nature, 2026. ista: Vargas Barroso VM, Watson J, Navas Olivé AC, Schlögl A, Jonas PM. 2026. Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit. Nature Communications. 17, 5540. mla: Vargas Barroso, Victor M., et al. “Developmental Emergence of Sparse and Structured Synaptic Connectivity in the Hippocampal CA3 Memory Circuit.” Nature Communications, vol. 17, 5540, Springer Nature, 2026, doi:10.1038/s41467-026-71914-x. short: V.M. Vargas Barroso, J. Watson, A.C. Navas Olivé, A. Schlögl, P.M. Jonas, Nature Communications 17 (2026). corr_author: '1' das_tickbox: '1' dataavailabilitystatement: Source data are provided with this paper. Additional original data are available from the corresponding author upon request. Code is available from https://doi.org/10.15479/AT-ISTA-21442 under the link https://research-explorer.ista.ac.at/download/21442/21443/ca3simu-vargas2026v1.tar.gz date_created: 2026-06-30T13:05:52Z date_published: 2026-06-23T00:00:00Z date_updated: 2026-07-01T06:47:49Z day: '23' ddc: - '570' department: - _id: PeJo - _id: ScienComp doi: 10.1038/s41467-026-71914-x ec_funded: 1 external_id: pmid: - '42014695' file: - access_level: open_access checksum: d0b0093493926985b4c268662ff4d556 content_type: application/pdf creator: dernst date_created: 2026-07-01T06:46:06Z date_updated: 2026-07-01T06:46:06Z file_id: '22231' file_name: 2026_NatureComm_VargasBarroso.pdf file_size: 18304997 relation: main_file success: 1 file_date_updated: 2026-07-01T06:46:06Z has_accepted_license: '1' intvolume: ' 17' language: - iso: eng month: '06' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 25B7EB9E-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '692692' name: Biophysics and circuit function of a giant cortical glutamatergic synapse - _id: e62b56fe-ab3c-11f0-94c7-d181dd352b3b grant_number: '101199096' name: Synaptic mechanisms of engram storage and retrieval in CA3 hippocampal microcircuits - _id: 260C2330-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '754411' name: ISTplus - Postdoctoral Fellowships - _id: fc2be41b-9c52-11eb-aca3-faa90aa144e9 call_identifier: H2020 grant_number: '101026635' name: Synaptic computations of the hippocampal CA3 circuitry - _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5 grant_number: P36232 name: Mechanisms of GABA release in hippocampal circuits - _id: 8d9195e9-16d5-11f0-9cad-d075be887a1e grant_number: PAT 4178023 name: Synaptic networks of human brain - _id: 26366136-B435-11E9-9278-68D0E5697425 name: Reglas de Conectividad funcional en el hipocampo publication: Nature Communications publication_identifier: eissn: - 2041-1723 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: record: - id: '21442' relation: research_data status: public researchdata_availability: yes scopus_import: '1' status: public supplementarymaterial: yes title: Developmental emergence of sparse and structured synaptic connectivity in the hippocampal CA3 memory circuit tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 17 year: '2026' ... --- _id: '21442' author: - first_name: Alois full_name: Schlögl, Alois id: 45BF87EE-F248-11E8-B48F-1D18A9856A87 last_name: Schlögl orcid: 0000-0002-5621-8100 citation: ama: Schlögl A. CA3Simu v1.06 (vargas2026v1). 2026. doi:10.15479/AT-ISTA-21442 apa: Schlögl, A. (2026). CA3Simu v1.06 (vargas2026v1). Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21442 chicago: Schlögl, Alois. “CA3Simu v1.06 (Vargas2026v1).” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21442. ieee: A. Schlögl, “CA3Simu v1.06 (vargas2026v1).” Institute of Science and Technology Austria, 2026. ista: Schlögl A. 2026. CA3Simu v1.06 (vargas2026v1), Institute of Science and Technology Austria, 10.15479/AT-ISTA-21442. mla: Schlögl, Alois. CA3Simu v1.06 (Vargas2026v1). Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-21442. short: A. Schlögl, (2026). corr_author: '1' date_created: 2026-03-12T08:20:46Z date_published: 2026-03-12T00:00:00Z date_updated: 2026-07-01T06:47:49Z day: '12' department: - _id: ScienComp - _id: PeJo doi: 10.15479/AT-ISTA-21442 ec_funded: 1 file: - access_level: open_access checksum: 441c8827717dcda05f91c127d15cf1e9 content_type: application/gzip creator: schloegl date_created: 2026-03-12T08:19:14Z date_updated: 2026-03-12T08:19:14Z file_id: '21443' file_name: ca3simu-vargas2026v1.tar.gz file_size: 160410 relation: main_file success: 1 - access_level: open_access checksum: 3c0092076228a15c0a7ae703192d43ea content_type: text/markdown creator: schloegl date_created: 2026-03-12T10:24:45Z date_updated: 2026-03-12T10:24:45Z file_id: '21445' file_name: README.md file_size: 10923 relation: main_file success: 1 file_date_updated: 2026-03-12T10:24:45Z has_accepted_license: '1' keyword: - hypocampus - ca3 simulations - modelling month: '03' oa: 1 project: - _id: e62b56fe-ab3c-11f0-94c7-d181dd352b3b grant_number: '101199096' name: Synaptic mechanisms of engram storage and retrieval in CA3 hippocampal microcircuits - _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5 grant_number: P36232 name: Mechanisms of GABA release in hippocampal circuits - _id: 8d9195e9-16d5-11f0-9cad-d075be887a1e grant_number: PAT 4178023 name: Synaptic networks of human brain - _id: 25B7EB9E-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '692692' name: Biophysics and circuit function of a giant cortical glutamatergic synapse publisher: Institute of Science and Technology Austria related_material: record: - id: '22229' relation: used_in_publication status: public status: public title: CA3Simu v1.06 (vargas2026v1) tmp: legal_code_url: https://www.gnu.org/licenses/gpl-3.0.en.html name: GNU General Public License 3.0 short: GPL 3.0 type: software user_id: 68b8ca59-c5b3-11ee-8790-cd641c68093d year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '22254' abstract: - lang: eng text: The global rise of antimicrobial resistance has intensified the search for new microbial metabolites from underexplored environments and taxonomic groups. Extreme and geographically isolated habitats such as Antarctic terrestrial ecosystems represent promising reservoirs of biosynthetic diversity, particularly among rare and difficult-to-cultivate actinomycetes that may produce chemically diverse metabolites with potential biotechnological applications. Here, we report the characterization of kineochelins, a previously undescribed group of siderophores produced by the Antarctic isolate Actinokineospora sp. UV203, representing a difficult-to-cultivate actinomycete lineage. Structural elucidation revealed a set of closely related congeners with a mixed-ligand architecture consistent with metal-chelating activity. Genome mining combined with transcriptomic analysis identified a dedicated nonribosomal peptide synthetase-encoding biosynthetic gene cluster responsible for kineochelin production. Comparative genomic analyses indicated that, although kineochelin biosynthetic genes share limited similarity with known mixed-ligand siderophores, their gene content and organization differ substantially, suggesting a distinct biosynthetic lineage. Functional characterization of the culture supernatant and an enriched pre-purified kineochelin fraction demonstrated strong and selective iron chelation, with high affinity for ferric and ferrous iron. Crude culture extracts inhibited the growth of bacterial strains isolated from the same Antarctic environment, indicating that kineochelins may contribute to iron-mediated microbial competition. In addition, kineochelin-enriched pre-purified fractions showed moderate selective inhibitory activity against the opportunistic yeast pathogen Nakaseomyces glabratus and a clinical isolate of Saccharomyces cerevisiae associated with invasive infection. These findings expand the chemical and biosynthetic diversity known within the genus Actinokineospora and demonstrate that Antarctic rare actinomycetes represent valuable sources of previously unexplored natural products. The discovery of kineochelins highlights the potential of genome-guided exploration of polar microorganisms for identifying bioactive metabolites with relevance for antimicrobial discovery and biotechnology. acknowledgement: This work was supported by the Czech Antarctic Research Programme 2025–2027 (VAN 2025) and the University of Vienna via the Research Platform Secondary Metabolomes of Bacterial Communities (MetaBac). S.K. has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 101020356 (DEFCOMANT, https://doi.org/10.3030/101020356) and MASH StG/CoG (MUNI/SC/1946/2024) by Masaryk University. T.R. and A.L. were funded in part by the Austrian Science Fund FWF [grant DOI https://doi.org/10.55776/COE7]. M.B. was funded by the Ministry of Health, Czech Republic—conceptual development of research organization (FNBr, 65269705). The Life Science Compute Cluster LiSC at the University of Vienna provided the high-performance computing infrastructure for this study. We thank Julia Ramesmayer and Sara Malinowski (Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna) for assistance during high molecular weight extraction and RNA extraction. The authors thank Anna Fabisikova and Michael Klemm-Abraham from the Mass Spectrometry Centre and the team of the NMR Centre (both core facilities of the Faculty of Chemistry, University of Vienna, and members of the Vienna Life Science Instruments) for assistance with data acquisition. We are thankful to Dr. Jaime Felipe Guerrero Garzón for helpful discussions on the use of a rrn operon promoter strategy. For open access purposes, the authors have applied for a CC BY public copyright licence to any author-accepted manuscript version arising from this submission. Dr. Martin Kello (Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia) and Dr. Michal Goga (Department of Plant Biology, Faculty of Science and Center for Interdisciplinary Biosciences, Technology and Innovation Park, Pavol Jozef Šafárik University in Košice, Košice, Slovakia), funded by VEGA 1/0498/23, are acknowledged for their assistance with the antiproliferative assays. This work was supported by Horizon 2020 Framework Programme, 101020356; Universität Wien, MetaBac; Ministry of Education, Youth and Sports, VAN 2025; Masarykova Univerzita, MUNI/SC/1946/2024; Austrian Science Fund, 10.55776/COE7; Ministerstvo Zdravotnictví České Republiky, FNBr, 65269705; Vedecká grantová agentúra Ministerstva školstva, výskumu, vývoja a mládeže Slovenskej republiky a Slovenskej akadémie vied, VEGA 1/0498/23. article_number: e70386 article_processing_charge: Yes article_type: original author: - first_name: Stanislava full_name: Kralova, Stanislava last_name: Kralova - first_name: Peter full_name: Spacek, Peter last_name: Spacek - first_name: Johannes full_name: Gafriller, Johannes last_name: Gafriller - first_name: Matej full_name: Bezdicek, Matej last_name: Bezdicek - first_name: Viktoria full_name: Medvedcova, Viktoria last_name: Medvedcova - first_name: Joana full_name: Séneca, Joana last_name: Séneca - first_name: Jay full_name: Osvatic, Jay last_name: Osvatic - first_name: Ulrike full_name: Grienke, Ulrike last_name: Grienke - first_name: Thomas full_name: Rattei, Thomas last_name: Rattei - first_name: Olga N. full_name: Sekurova, Olga N. last_name: Sekurova - first_name: Sergey B. full_name: Zotchev, Sergey B. last_name: Zotchev - first_name: Martin full_name: Zehl, Martin id: 8e016d5b-5d77-11f0-86d2-96cdb3922a55 last_name: Zehl orcid: 0000-0001-9685-0373 - first_name: Alexander full_name: Loy, Alexander last_name: Loy biorxivid: 1 citation: ama: Kralova S, Spacek P, Gafriller J, et al. Kineochelins - A new group of siderophores from an antarctic bacterium. Microbial Biotechnology. 2026;19(6). doi:10.1111/1751-7915.70386 apa: Kralova, S., Spacek, P., Gafriller, J., Bezdicek, M., Medvedcova, V., Séneca, J., … Loy, A. (2026). Kineochelins - A new group of siderophores from an antarctic bacterium. Microbial Biotechnology. Wiley. https://doi.org/10.1111/1751-7915.70386 chicago: Kralova, Stanislava, Peter Spacek, Johannes Gafriller, Matej Bezdicek, Viktoria Medvedcova, Joana Séneca, Jay Osvatic, et al. “Kineochelins - A New Group of Siderophores from an Antarctic Bacterium.” Microbial Biotechnology. Wiley, 2026. https://doi.org/10.1111/1751-7915.70386. ieee: S. Kralova et al., “Kineochelins - A new group of siderophores from an antarctic bacterium,” Microbial Biotechnology, vol. 19, no. 6. Wiley, 2026. ista: Kralova S, Spacek P, Gafriller J, Bezdicek M, Medvedcova V, Séneca J, Osvatic J, Grienke U, Rattei T, Sekurova ON, Zotchev SB, Zehl M, Loy A. 2026. Kineochelins - A new group of siderophores from an antarctic bacterium. Microbial Biotechnology. 19(6), e70386. mla: Kralova, Stanislava, et al. “Kineochelins - A New Group of Siderophores from an Antarctic Bacterium.” Microbial Biotechnology, vol. 19, no. 6, e70386, Wiley, 2026, doi:10.1111/1751-7915.70386. short: S. Kralova, P. Spacek, J. Gafriller, M. Bezdicek, V. Medvedcova, J. Séneca, J. Osvatic, U. Grienke, T. Rattei, O.N. Sekurova, S.B. Zotchev, M. Zehl, A. Loy, Microbial Biotechnology 19 (2026). corr_author: '1' das_tickbox: '1' dataavailabilitystatement: The genome sequence and transcriptomic data of strain Actinokineospora sp. UV203 are available on NCBI (BioProject accession number PRJNA1331526). The nearly full-length 16S rRNA gene (1395 bp) of strain Actinokineospora sp. UV203 is available on NCBI (accession number PX090945). The NMR data of kineochelin E1 and A1 are deposited in the Natural Products Magnetic Resonance Database (NP-MRD) under accession numbers NP0352113 and NP0352114, respectively. date_created: 2026-07-08T09:19:43Z date_published: 2026-06-01T00:00:00Z date_updated: 2026-07-13T06:59:08Z day: '01' ddc: - '570' department: - _id: MassSpec doi: 10.1111/1751-7915.70386 external_id: biorxivid: - 10.64898/2026.02.23.707395 pmid: - '42210522' file: - access_level: open_access checksum: 4f735714644f1049b22b014225843d8d content_type: application/pdf creator: dernst date_created: 2026-07-13T06:57:19Z date_updated: 2026-07-13T06:57:19Z file_id: '22271' file_name: 2026_MicrobialBiotechnology_Kralova.pdf file_size: 2497486 relation: main_file success: 1 file_date_updated: 2026-07-13T06:57:19Z has_accepted_license: '1' intvolume: ' 19' issue: '6' keyword: - Actinokineospora - Antarctica - antimicrobial discovery - biosynthetic gene cluster - genome mining - microbial competition - nonribosomalpeptide synthetase - siderophores language: - iso: eng month: '06' oa: 1 oa_version: Published Version pmid: 1 publication: Microbial Biotechnology publication_identifier: eissn: - 1751-7915 publication_status: published publisher: Wiley quality_controlled: '1' scopus_import: '1' status: public supplementarymaterial: yes title: Kineochelins - A new group of siderophores from an antarctic bacterium tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 19 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '22289' abstract: - lang: eng text: Single-stranded, helically folded aromatic oligoamides bearing anionic phosphonate side chains have been shown to bind to some DNA-binding proteins better than DNA itself. However, these DNA mimic foldamers have until now mainly consisted of a single repeat motif, like a poly(dA:dT) DNA duplex, and contained limited sequence information. Here, we introduce new monomers designed to display different chemical functionalities in the major groove of the DNA mimics. Four new Fmoc-protected amino acid monomers have been synthesized and incorporated into oligomers. Sixteen foldamer sequences were prepared on solid phase. Their conformations in solution and in the solid state and their conformational dynamics were investigated using nuclear magnetic resonance, circular dichroism, molecular modeling, and X-ray crystallography. The results show that three of the four new monomers behaved as designed and that their introduction enhances the conformational dynamics of the DNA mimic foldamers. In a fourth case, conformational behavior proved to be more complex than expected. The modified sequences retained the ability to bind to the bacterial histone-like protein HU. These results showcase design strategies to manipulate large molecular biomimetics in which not only side chains but also main chain components are varied. The new monomers pave the way to complex DNA mimic foldamer sequences targeting proteins that recognize sequence-selective DNA-binding proteins such as transcription factors or restriction enzymes. acknowledgement: We acknowledge financial support from the European Research Council (ERC) under the European Union's Horizon Europe Framework Programme (grant agreement no. ERC-2021-ADG-320892) and from the China Scholarship Council (CSC, predoctoral fellowship to J. W.). We thank L. Allmendinger for assistance with NMR measurements, P. Mayer for his assistance in solving the crystal structures of 1 and 1d, L. Bodero for assistance with automated solid-phase synthesis, M. Rogovoi for providing monomer precursors, and M. Loos for the purification and analysis of compounds 15a–19a. We thank M. Soler-Lopez (ID23-1, ESRF, Grenoble) and I. Bento (EMBL P13, Petra III, DESY, Hamburg) for assistance during data collection at the synchrotron beamlines. article_processing_charge: Yes article_type: original author: - first_name: Jiaojiao full_name: Wu, Jiaojiao last_name: Wu - first_name: Valentina full_name: Corvaglia, Valentina last_name: Corvaglia - first_name: Tulika full_name: Chakrabortty, Tulika last_name: Chakrabortty - first_name: Pradeep K full_name: Mandal, Pradeep K id: 6a3def15-d4b4-11ef-9fa9-a24c1f545ec3 last_name: Mandal orcid: 0000-0001-5996-956X - first_name: Ivan full_name: Huc, Ivan last_name: Huc citation: ama: Wu J, Corvaglia V, Chakrabortty T, Mandal PK, Huc I. Tailoring the major groove of DNA mimic foldamers. Chemical Science. doi:10.1039/d6sc00798h apa: Wu, J., Corvaglia, V., Chakrabortty, T., Mandal, P. K., & Huc, I. (n.d.). Tailoring the major groove of DNA mimic foldamers. Chemical Science. Royal Society of Chemistry. https://doi.org/10.1039/d6sc00798h chicago: Wu, Jiaojiao, Valentina Corvaglia, Tulika Chakrabortty, Pradeep K Mandal, and Ivan Huc. “Tailoring the Major Groove of DNA Mimic Foldamers.” Chemical Science. Royal Society of Chemistry, n.d. https://doi.org/10.1039/d6sc00798h. ieee: J. Wu, V. Corvaglia, T. Chakrabortty, P. K. Mandal, and I. Huc, “Tailoring the major groove of DNA mimic foldamers,” Chemical Science. Royal Society of Chemistry. ista: Wu J, Corvaglia V, Chakrabortty T, Mandal PK, Huc I. Tailoring the major groove of DNA mimic foldamers. Chemical Science. mla: Wu, Jiaojiao, et al. “Tailoring the Major Groove of DNA Mimic Foldamers.” Chemical Science, Royal Society of Chemistry, doi:10.1039/d6sc00798h. short: J. Wu, V. Corvaglia, T. Chakrabortty, P.K. Mandal, I. Huc, Chemical Science (n.d.). das_tickbox: '1' dataavailabilitystatement: "CCDC 2514117, 2514118, 2286782 and 2478322 (compound 1, compound 1d, oligomer 5, and oligomer 6, respectively) contain the supplementary crystallographic data for this paper.54a–d \r\n\r\nThe supporting data have been provided as part of the supplementary information (SI). Supplementary information: SI figures, detailed experimental protocols, crystallographic studies, and characterisation of new compounds. See DOI: https://doi.org/10.1039/d6sc00798h." date_created: 2026-07-13T09:41:36Z date_published: 2026-06-09T00:00:00Z date_updated: 2026-07-13T11:24:29Z day: '09' ddc: - '540' department: - _id: LifeSc doi: 10.1039/d6sc00798h has_accepted_license: '1' language: - iso: eng month: '06' oa_version: Published Version publication: Chemical Science publication_identifier: eissn: - 2041-6539 issn: - 2041-6520 publication_status: inpress publisher: Royal Society of Chemistry quality_controlled: '1' researchdata_availability: yes scopus_import: '1' status: public supplementarymaterial: yes title: Tailoring the major groove of DNA mimic foldamers tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '22301' abstract: - lang: eng text: Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage. acknowledged_ssus: - _id: Bio - _id: NanoFab acknowledgement: Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria. article_number: jipb.70309 article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Adrijana full_name: Smoljan, Adrijana id: cced8a85-223e-11ed-af04-b0596c55053b last_name: Smoljan - first_name: Sarah full_name: Koutnik‐Abele, Sarah last_name: Koutnik‐Abele - first_name: Dmitrii full_name: Vladimirtsev, Dmitrii id: 60466724-5355-11ee-ae5a-fa55e8f99c3d last_name: Vladimirtsev - first_name: Petr full_name: Klíma, Petr last_name: Klíma - first_name: Anita full_name: Bírošíková, Anita last_name: Bírošíková - first_name: Yuzhou full_name: Zhang, Yuzhou id: 3B6137F2-F248-11E8-B48F-1D18A9856A87 last_name: Zhang orcid: 0000-0003-2627-6956 - first_name: Jack full_name: Merrin, Jack id: 4515C308-F248-11E8-B48F-1D18A9856A87 last_name: Merrin orcid: 0000-0001-5145-4609 - first_name: Maximilian full_name: Schuster, Maximilian id: 37e65def-d415-11eb-ae59-a7b67be103db last_name: Schuster - first_name: Katarina full_name: Kurtović, Katarina last_name: Kurtović - first_name: Ulrich Z. full_name: Hammes, Ulrich Z. last_name: Hammes - first_name: Jan full_name: Petrášek, Jan last_name: Petrášek - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology. 2026. doi:10.1111/jipb.70309 apa: Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology. Wiley. https://doi.org/10.1111/jipb.70309 chicago: Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology. Wiley, 2026. https://doi.org/10.1111/jipb.70309. ieee: A. Smoljan et al., “Auxin response and PIN‐mediated transport in chlorophyte algae,” Journal of Integrative Plant Biology. Wiley, 2026. ista: Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309. mla: Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology, jipb. 70309, Wiley, 2026, doi:10.1111/jipb.70309. short: A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026). corr_author: '1' das_tickbox: '0' date_created: 2026-07-13T10:44:55Z date_published: 2026-06-10T00:00:00Z date_updated: 2026-07-13T14:26:31Z day: '10' ddc: - '580' department: - _id: JiFr - _id: GradSch - _id: NanoFab - _id: Bio doi: 10.1111/jipb.70309 external_id: pmid: - '42271607' has_accepted_license: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1111/jipb.70309 month: '06' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants - _id: bd76d395-d553-11ed-ba76-f678c14f9033 grant_number: I06123 name: Peptide receptors for auxin canalization in Arabidopsis - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors publication: Journal of Integrative Plant Biology publication_identifier: eissn: - 1744-7909 issn: - 1672-9072 publication_status: epub_ahead publisher: Wiley quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: yes title: Auxin response and PIN‐mediated transport in chlorophyte algae tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '20859' abstract: - lang: eng text: Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo. acknowledged_ssus: - _id: NanoFab acknowledgement: 'The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union''s Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.' article_processing_charge: Yes (in subscription journal) article_type: original author: - first_name: Iván full_name: Company-Garrido, Iván last_name: Company-Garrido - first_name: Alberto full_name: Zurita Carpio, Alberto last_name: Zurita Carpio - first_name: Mariona full_name: Colomer-Rosell, Mariona last_name: Colomer-Rosell - first_name: Bernard full_name: Ciraulo, Bernard last_name: Ciraulo - first_name: Ronja full_name: Molkenbur, Ronja last_name: Molkenbur - first_name: Peter full_name: Lanzerstorfer, Peter last_name: Lanzerstorfer - first_name: Fabio full_name: Pezzano, Fabio last_name: Pezzano - first_name: Costanza full_name: Agazzi, Costanza last_name: Agazzi - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Saumey full_name: Jain, Saumey last_name: Jain - first_name: Jeroen M. full_name: Jacques, Jeroen M. last_name: Jacques - first_name: Valeria full_name: Venturini, Valeria last_name: Venturini - first_name: Christian full_name: Knapp, Christian last_name: Knapp - first_name: Yufei full_name: Xie, Yufei last_name: Xie - first_name: Jack full_name: Merrin, Jack id: 4515C308-F248-11E8-B48F-1D18A9856A87 last_name: Merrin orcid: 0000-0001-5145-4609 - first_name: Julian full_name: Weghuber, Julian last_name: Weghuber - first_name: Marcel full_name: Schaaf, Marcel last_name: Schaaf - first_name: Romain full_name: Quidant, Romain last_name: Quidant - first_name: Eva full_name: Kiermaier, Eva id: 3EB04B78-F248-11E8-B48F-1D18A9856A87 last_name: Kiermaier orcid: 0000-0001-6165-5738 - first_name: Jaime full_name: Ortega Arroyo, Jaime last_name: Ortega Arroyo - first_name: Verena full_name: Ruprecht, Verena id: 4D71A03A-F248-11E8-B48F-1D18A9856A87 last_name: Ruprecht orcid: 0000-0003-4088-8633 - first_name: Stefan full_name: Wieser, Stefan id: 355AA5A0-F248-11E8-B48F-1D18A9856A87 last_name: Wieser orcid: 0000-0002-2670-2217 citation: ama: Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 2026;61(2):356-371.e12. doi:10.1016/j.devcel.2025.10.006 apa: Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.10.006 chicago: Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” Developmental Cell. Elsevier, 2026. https://doi.org/10.1016/j.devcel.2025.10.006. ieee: I. Company-Garrido et al., “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” Developmental Cell, vol. 61, no. 2. Elsevier, p. 356–371.e12, 2026. ista: Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12. mla: Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” Developmental Cell, vol. 61, no. 2, Elsevier, 2026, p. 356–371.e12, doi:10.1016/j.devcel.2025.10.006. short: I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61 (2026) 356–371.e12. das_tickbox: '1' dataavailabilitystatement: "This study did not generate new unique reagents. Data are available upon request.\r\n•The custom-made codes used in this study are available at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request." date_created: 2025-12-28T23:01:27Z date_published: 2026-02-11T00:00:00Z date_updated: 2026-07-23T06:27:15Z day: '11' ddc: - '570' department: - _id: Bio - _id: NanoFab doi: 10.1016/j.devcel.2025.10.006 external_id: pmid: - '41192429' file: - access_level: open_access checksum: 52fd52d2d19a4514f8fcc1b40f420ca2 content_type: application/pdf creator: dernst date_created: 2026-07-23T06:26:25Z date_updated: 2026-07-23T06:26:25Z file_id: '22388' file_name: 2026_DevelopmentalCell_CompanyGarrido.pdf file_size: 12342817 relation: main_file success: 1 file_date_updated: 2026-07-23T06:26:25Z has_accepted_license: '1' intvolume: ' 61' issue: '2' keyword: - thermobiology - cell migration - thermo-adaptability of immune cells language: - iso: eng month: '02' oa: 1 oa_version: Published Version page: 356-371.e12 pmid: 1 publication: Developmental Cell publication_identifier: eissn: - 1878-1551 issn: - 1534-5807 publication_status: published publisher: Elsevier quality_controlled: '1' researchdata_availability: upon request scopus_import: '1' status: public supplementarymaterial: yes title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 61 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '21883' abstract: - lang: eng text: Three-dimensional (3D) printing has rapidly developed from a niche hobbyist activity into a widely accessible and indispensable technology across multiple scientific disciplines. Within microscopy, optical engineering laboratories and imaging core facilities, 3D printing enables creating customised solutions for sample holders, optical components and everyday laboratory tools that traditionally required specialised machining. By providing rapid prototyping, low-cost production and reproducibility, 3D printing facilitates innovation and efficiency in facility operations. This article provides a perspective on the possibilities, challenges, and practical aspects of implementing 3D printing within microscopy core facilities. Instead of providing technical review about 3D printing, we focus on service organisation, user engagement, resource management and community-driven repositories for design dissemination. Our aim is to share insights with those considering the implementation of 3D printing as a service for developing add-on components to ease the operation of different aspects of the machine-park driven services and those who are managing advanced instrumentation within research groups. acknowledged_ssus: - _id: Bio - _id: M-Shop acknowledgement: "This work was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Imaging & Optics Facility (IOF) and the MiBa Machine Shop. Specifically; Robert Hauschild (IOF), sharing designs, insights and pioneering 3D printing activities at the Imaging and Optics Facility; Bernhard Hochreiter (IOF), for support and testing of anoxic chamber. We also thank Ana Rita Carvalho Faria and Oliver Biehlmaier (Biozentrum University of Basel, Imaging Core Facility) for sharing the design of the adopted power meter.\r\nOpen Access funding provided by Institute of Science and Technology Austria." article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Mohammad full_name: Goudarzi, Mohammad id: 3384113A-F248-11E8-B48F-1D18A9856A87 last_name: Goudarzi - first_name: Maximilian full_name: Schuster, Maximilian id: 37e65def-d415-11eb-ae59-a7b67be103db last_name: Schuster - first_name: Arthur full_name: Milberger, Arthur last_name: Milberger - first_name: Manuel full_name: Gunkel, Manuel last_name: Gunkel - first_name: Stefan full_name: Terjung, Stefan last_name: Terjung - first_name: Gabriel full_name: Krens, Gabriel id: 2B819732-F248-11E8-B48F-1D18A9856A87 last_name: Krens orcid: 0000-0003-4761-5996 citation: ama: Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 3D printing in core facilities – Low pain, high gain. Journal of Microscopy. 2026;302(3):382-395. doi:10.1111/jmi.70106 apa: Goudarzi, M., Schuster, M., Milberger, A., Gunkel, M., Terjung, S., & Krens, G. (2026). 3D printing in core facilities – Low pain, high gain. Journal of Microscopy. Wiley. https://doi.org/10.1111/jmi.70106 chicago: Goudarzi, Mohammad, Maximilian Schuster, Arthur Milberger, Manuel Gunkel, Stefan Terjung, and Gabriel Krens. “3D Printing in Core Facilities – Low Pain, High Gain.” Journal of Microscopy. Wiley, 2026. https://doi.org/10.1111/jmi.70106. ieee: M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, and G. Krens, “3D printing in core facilities – Low pain, high gain,” Journal of Microscopy, vol. 302, no. 3. Wiley, pp. 382–395, 2026. ista: Goudarzi M, Schuster M, Milberger A, Gunkel M, Terjung S, Krens G. 2026. 3D printing in core facilities – Low pain, high gain. Journal of Microscopy. 302(3), 382–395. mla: Goudarzi, Mohammad, et al. “3D Printing in Core Facilities – Low Pain, High Gain.” Journal of Microscopy, vol. 302, no. 3, Wiley, 2026, pp. 382–95, doi:10.1111/jmi.70106. short: M. Goudarzi, M. Schuster, A. Milberger, M. Gunkel, S. Terjung, G. Krens, Journal of Microscopy 302 (2026) 382–395. corr_author: '1' das_tickbox: '0' date_created: 2026-05-17T22:02:11Z date_published: 2026-06-01T00:00:00Z date_updated: 2026-07-27T14:02:46Z day: '01' ddc: - '600' department: - _id: Bio doi: 10.1111/jmi.70106 external_id: pmid: - '42104760' file: - access_level: open_access checksum: 06dfad92b1465ed614a1201b4129960a content_type: application/pdf creator: dernst date_created: 2026-07-27T14:01:34Z date_updated: 2026-07-27T14:01:34Z file_id: '22593' file_name: 2026_JourMicroscopy_Goudarzi.pdf file_size: 4625767 relation: main_file success: 1 file_date_updated: 2026-07-27T14:01:34Z has_accepted_license: '1' intvolume: ' 302' issue: '3' language: - iso: eng month: '06' oa: 1 oa_version: Published Version page: 382-395 pmid: 1 publication: Journal of Microscopy publication_identifier: eissn: - 1365-2818 issn: - 0022-2720 publication_status: published publisher: Wiley quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: no title: 3D printing in core facilities – Low pain, high gain tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 302 year: '2026' ... --- OA_type: closed access _id: '21950' abstract: - lang: eng text: One Health initiatives are modern paradigms for research and health care practices in various fields. Concrete definitions of the One Health framework, however, remain heterogeneous, leading to conceptual problems and uncertainties in the application of the framework. This article discusses several approaches to the One Health concept, and their associated consequences, with special focus on animal experimentation. The first issue addressed is how One Health should be defined, as well as what (and who) should be considered within a One Health approach. In order to shed further light on this, we explore the history of animals in biomedical science, highlighting historical milestones in the use of animal models, as well as the development and current state of ethical considerations in the field of animal experimentation. The second issue comes with the inclusion of animal experimentation per se as part of the One Health concept. Therefore, particular attention is paid to bioethical principles and the resulting problems that can arise when applying them to the One Health concept. Arguments such as the idea of inequality between humans and non-human animals, and the premise that all actions are done for the benefit of humans, are raised and then used to explore the question of whether the One Health concept is compatible with existing bioethical principles. Based on the bioethical principles of protecting the environment, the biodiversity and biosphere, this paper seeks an inclusive perspective of the One Health concept. Successful solutions will be based on this concept, which embraces all living beings. The authors conclude that a multispecies ethics approach could help create a more ethical ecosystem that is aligned with the wellbeing of all life on a shared planet. article_processing_charge: No article_type: original author: - first_name: Yesim Isil full_name: Ulman, Yesim Isil last_name: Ulman - first_name: Nikos full_name: Kostomitsopoulos, Nikos last_name: Kostomitsopoulos - first_name: Samuel full_name: Camenzind, Samuel last_name: Camenzind - first_name: Maria full_name: Kitsara, Maria last_name: Kitsara - first_name: Ilja Richard full_name: Pavone, Ilja Richard last_name: Pavone - first_name: Sophie full_name: Schober, Sophie id: 80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8 last_name: Schober citation: ama: 'Ulman YI, Kostomitsopoulos N, Camenzind S, Kitsara M, Pavone IR, Schober S. Emerging bioethical conflicts: One Health and animal experimentation. Alternatives to Laboratory Animals. 2026;54(4):226-235. doi:10.1177/02611929261453330' apa: 'Ulman, Y. I., Kostomitsopoulos, N., Camenzind, S., Kitsara, M., Pavone, I. R., & Schober, S. (2026). Emerging bioethical conflicts: One Health and animal experimentation. Alternatives to Laboratory Animals. SAGE Publications. https://doi.org/10.1177/02611929261453330' chicago: 'Ulman, Yesim Isil, Nikos Kostomitsopoulos, Samuel Camenzind, Maria Kitsara, Ilja Richard Pavone, and Sophie Schober. “Emerging Bioethical Conflicts: One Health and Animal Experimentation.” Alternatives to Laboratory Animals. SAGE Publications, 2026. https://doi.org/10.1177/02611929261453330.' ieee: 'Y. I. Ulman, N. Kostomitsopoulos, S. Camenzind, M. Kitsara, I. R. Pavone, and S. Schober, “Emerging bioethical conflicts: One Health and animal experimentation,” Alternatives to Laboratory Animals, vol. 54, no. 4. SAGE Publications, pp. 226–235, 2026.' ista: 'Ulman YI, Kostomitsopoulos N, Camenzind S, Kitsara M, Pavone IR, Schober S. 2026. Emerging bioethical conflicts: One Health and animal experimentation. Alternatives to Laboratory Animals. 54(4), 226–235.' mla: 'Ulman, Yesim Isil, et al. “Emerging Bioethical Conflicts: One Health and Animal Experimentation.” Alternatives to Laboratory Animals, vol. 54, no. 4, SAGE Publications, 2026, pp. 226–35, doi:10.1177/02611929261453330.' short: Y.I. Ulman, N. Kostomitsopoulos, S. Camenzind, M. Kitsara, I.R. Pavone, S. Schober, Alternatives to Laboratory Animals 54 (2026) 226–235. corr_author: '1' das_tickbox: '1' date_created: 2026-06-07T22:01:36Z date_published: 2026-07-01T00:00:00Z date_updated: 2026-07-27T14:13:17Z day: '01' department: - _id: PreCl doi: 10.1177/02611929261453330 external_id: pmid: - '42185081' intvolume: ' 54' issue: '4' language: - iso: eng month: '07' oa_version: None page: 226-235 pmid: 1 publication: Alternatives to Laboratory Animals publication_identifier: eissn: - 2632-3559 issn: - 0261-1929 publication_status: published publisher: SAGE Publications quality_controlled: '1' scopus_import: '1' status: public title: 'Emerging bioethical conflicts: One Health and animal experimentation' type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 54 year: '2026' ...