--- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '23171' abstract: - lang: eng text: Crawling motility is a hallmark of eukaryotic cells and requires a dynamic actin cytoskeleton, regulated adhesion and spatially organized signalling pathways1,2. Asgard archaea, which are considered the closest known prokaryotic relatives of eukaryotes, potentially encode these functions within their large set of ‘eukaryotic signature proteins’3,4,5,6. The few cultivated members show a complex cell morphology, consisting of a central cell body from which several protrusions extend, filled with an actin-based cytoskeleton7,8. Here, live-cell microscopy of two organisms of the Lokiarchaea and Hodarchaea lineages7,9 showed that they dynamically and greatly change their cell shape on a minute time scale and grow and retract their extensive protrusions with a speed of 1.5–4.8 µm min−1, respectively. After adhering to a glass surface, cells use their protrusions to undergo active crawling motion. In the presence of selected actin inhibitors, however, the observed dynamics were arrested, suggesting a central role of actin in these processes. The observed cellular plasticity and motility are unique features among prokaryotes and might have been crucial for the emergence of the first eukaryotic cells that are thought to have formed through the association of a member of the Asgard archaea and an α-proteobacterium, the ancestor of mitochondria. acknowledged_ssus: - _id: Bio acknowledgement: This work would not have been possible without the technical support in cultivation from K. Hager, F. Baraket, A. Malits and S. Harrer. We thank the Imaging and Optics Facility (IOF) at ISTA for their help in setting up the anaerobic environment during imaging and the Cell Imaging & Ultrastructure Research (CIUS) facility at the University of Vienna for their support. Proteomics analyses were performed by the Mass Spectrometry Facility at Max Perutz Labs using the VBCF instrument pool. We also thank P. Klahn (University of Gothenburg, Sweden), M. Stadler and members of his department (HZI Braunschweig) as well as R. Cox (LUH Hannover) for providing different cytochalasans and P. Bieling (King’s College London) for discussion. Most of this work was funded by the Austrian Science Fund (FWF) with grant DOI 10.55776/Z437 (available at https://doi.org/10.55776/Z437) and further supported by an emerging field grant with grant DOI 10.55776/EFP25 (available at https://doi.org/10.55776/EFP25) to C.S. For open access purposes, the author has applied a CC BY public copyright license to any author accepted manuscript version arising from this submission. This work was further supported by a European Research Council grant ERC-SyG (101071793) to M.S., by the German Research Council through the CytoLabs consortium (Research Unit 5170 to K.R. and T.E.B.S.), by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research 22H04985 to H.I. and M.N. Furthermore, R.H. was supported by CZI grant DAF2020-225401 and a grant (https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF. Open access funding provided by University of Vienna. article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Philipp full_name: Radler, Philipp id: 40136C2A-F248-11E8-B48F-1D18A9856A87 last_name: Radler orcid: '0000-0001-9198-2182 ' - first_name: Tobias full_name: Viehboeck, Tobias last_name: Viehboeck - first_name: Zhen-Hao full_name: Luo, Zhen-Hao last_name: Luo - first_name: Nevena full_name: Maslać, Nevena last_name: Maslać - first_name: Katharina full_name: Schmidt, Katharina last_name: Schmidt - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Masaru K. full_name: Nobu, Masaru K. last_name: Nobu - first_name: Silvia full_name: Bulgheresi, Silvia last_name: Bulgheresi - first_name: Theresia E. B. full_name: Stradal, Theresia E. B. last_name: Stradal - first_name: Klemens full_name: Rottner, Klemens last_name: Rottner - first_name: Hiroyuki full_name: Imachi, Hiroyuki last_name: Imachi - first_name: Michael K full_name: Sixt, Michael K id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87 last_name: Sixt orcid: 0000-0002-6620-9179 - first_name: Christa full_name: Schleper, Christa last_name: Schleper citation: ama: Radler P, Viehboeck T, Luo Z-H, et al. Dynamic protrusions mediate crawling motility in Asgard archaea. Nature. 2026. doi:10.1038/s41586-026-11063-9 apa: Radler, P., Viehboeck, T., Luo, Z.-H., Maslać, N., Schmidt, K., Hauschild, R., … Schleper, C. (2026). Dynamic protrusions mediate crawling motility in Asgard archaea. Nature. Springer Nature. https://doi.org/10.1038/s41586-026-11063-9 chicago: Radler, Philipp, Tobias Viehboeck, Zhen-Hao Luo, Nevena Maslać, Katharina Schmidt, Robert Hauschild, Masaru K. Nobu, et al. “Dynamic Protrusions Mediate Crawling Motility in Asgard Archaea.” Nature. Springer Nature, 2026. https://doi.org/10.1038/s41586-026-11063-9. ieee: P. Radler et al., “Dynamic protrusions mediate crawling motility in Asgard archaea,” Nature. Springer Nature, 2026. ista: Radler P, Viehboeck T, Luo Z-H, Maslać N, Schmidt K, Hauschild R, Nobu MK, Bulgheresi S, Stradal TEB, Rottner K, Imachi H, Sixt MK, Schleper C. 2026. Dynamic protrusions mediate crawling motility in Asgard archaea. Nature. mla: Radler, Philipp, et al. “Dynamic Protrusions Mediate Crawling Motility in Asgard Archaea.” Nature, Springer Nature, 2026, doi:10.1038/s41586-026-11063-9. short: P. Radler, T. Viehboeck, Z.-H. Luo, N. Maslać, K. Schmidt, R. Hauschild, M.K. Nobu, S. Bulgheresi, T.E.B. Stradal, K. Rottner, H. Imachi, M.K. Sixt, C. Schleper, Nature (2026). das_tickbox: '1' dataavailabilitystatement: The plots in this manuscript were created using Jupyter Notebook; the source data for all figures have been provided in data source tables. Microscopy raw data for supplementary videos, the Jupyter notebooks to generate the plots and the raw files for the structural phylogenetic analysis are available at https://doi.org/10.6084/m9.figshare.30676367. The remaining raw microscopy data generated for this manuscript are available from https://www.ebi.ac.uk/biostudies/bioimages/studies/S-BIAD3928. The mass spectrometry proteomics data from the Co-IP experiments have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository79 and are available at https://www.ebi.ac.uk/pride/archive/projects/PXD068820. The mass spectrometry proteomics data of the proteome analysis is available at https://www.ebi.ac.uk/pride/archive/projects/PXD074767. Source data are provided with this paper. date_created: 2026-10-19T09:26:53Z date_published: 2026-09-30T00:00:00Z date_updated: 2026-10-19T09:40:50Z day: '30' ddc: - '570' department: - _id: MiSi - _id: Bio doi: 10.1038/s41586-026-11063-9 external_id: pmid: - '42816618' fulldoi: https://doi.org/10.1038/s41586-026-11063-9 has_accepted_license: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1038/s41586-026-11063-9 month: '09' oa: 1 oa_version: Published Version pmid: 1 project: - _id: bd91e723-d553-11ed-ba76-fe7eeb2185fd grant_number: '101071793' name: 'Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces' - _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473 grant_number: CZI01 name: Tools for automation and feedback microscopy publication: Nature publication_identifier: eissn: - 1476-4687 issn: - 0028-0836 publication_status: epub_ahead publisher: Springer Nature quality_controlled: '1' researchdata_availability: yes scopus_import: '1' status: public supplementarymaterial: yes title: Dynamic protrusions mediate crawling motility in Asgard archaea tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2026' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '20859' abstract: - lang: eng text: Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo. acknowledged_ssus: - _id: NanoFab acknowledgement: 'The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union''s Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.' article_processing_charge: Yes (in subscription journal) article_type: original author: - first_name: Iván full_name: Company-Garrido, Iván last_name: Company-Garrido - first_name: Alberto full_name: Zurita Carpio, Alberto last_name: Zurita Carpio - first_name: Mariona full_name: Colomer-Rosell, Mariona last_name: Colomer-Rosell - first_name: Bernard full_name: Ciraulo, Bernard last_name: Ciraulo - first_name: Ronja full_name: Molkenbur, Ronja last_name: Molkenbur - first_name: Peter full_name: Lanzerstorfer, Peter last_name: Lanzerstorfer - first_name: Fabio full_name: Pezzano, Fabio last_name: Pezzano - first_name: Costanza full_name: Agazzi, Costanza last_name: Agazzi - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Saumey full_name: Jain, Saumey last_name: Jain - first_name: Jeroen M. full_name: Jacques, Jeroen M. last_name: Jacques - first_name: Valeria full_name: Venturini, Valeria last_name: Venturini - first_name: Christian full_name: Knapp, Christian last_name: Knapp - first_name: Yufei full_name: Xie, Yufei last_name: Xie - first_name: Jack full_name: Merrin, Jack id: 4515C308-F248-11E8-B48F-1D18A9856A87 last_name: Merrin orcid: 0000-0001-5145-4609 - first_name: Julian full_name: Weghuber, Julian last_name: Weghuber - first_name: Marcel full_name: Schaaf, Marcel last_name: Schaaf - first_name: Romain full_name: Quidant, Romain last_name: Quidant - first_name: Eva full_name: Kiermaier, Eva id: 3EB04B78-F248-11E8-B48F-1D18A9856A87 last_name: Kiermaier orcid: 0000-0001-6165-5738 - first_name: Jaime full_name: Ortega Arroyo, Jaime last_name: Ortega Arroyo - first_name: Verena full_name: Ruprecht, Verena id: 4D71A03A-F248-11E8-B48F-1D18A9856A87 last_name: Ruprecht orcid: 0000-0003-4088-8633 - first_name: Stefan full_name: Wieser, Stefan id: 355AA5A0-F248-11E8-B48F-1D18A9856A87 last_name: Wieser orcid: 0000-0002-2670-2217 citation: ama: Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 2026;61(2):356-371.e12. doi:10.1016/j.devcel.2025.10.006 apa: Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2025.10.006 chicago: Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” Developmental Cell. Cell Press, 2026. https://doi.org/10.1016/j.devcel.2025.10.006. ieee: I. Company-Garrido et al., “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” Developmental Cell, vol. 61, no. 2. Cell Press, p. 356–371.e12, 2026. ista: Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12. mla: Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” Developmental Cell, vol. 61, no. 2, Cell Press, 2026, p. 356–371.e12, doi:10.1016/j.devcel.2025.10.006. short: I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61 (2026) 356–371.e12. das_tickbox: '1' dataavailabilitystatement: "This study did not generate new unique reagents. Data are available upon request.\r\n•The custom-made codes used in this study are available at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request." date_created: 2025-12-28T23:01:27Z date_published: 2026-02-11T00:00:00Z date_updated: 2026-10-20T09:01:14Z day: '11' ddc: - '570' department: - _id: Bio - _id: NanoFab doi: 10.1016/j.devcel.2025.10.006 external_id: pmid: - '41192429' file: - access_level: open_access checksum: 52fd52d2d19a4514f8fcc1b40f420ca2 content_type: application/pdf creator: dernst date_created: 2026-07-23T06:26:25Z date_updated: 2026-07-23T06:26:25Z file_id: '22388' file_name: 2026_DevelopmentalCell_CompanyGarrido.pdf file_size: 12342817 relation: main_file success: 1 file_date_updated: 2026-07-23T06:26:25Z fulldoi: https://doi.org/10.1016/j.devcel.2025.10.006 has_accepted_license: '1' intvolume: ' 61' issue: '2' keyword: - thermobiology - cell migration - thermo-adaptability of immune cells language: - iso: eng month: '02' oa: 1 oa_version: Published Version page: 356-371.e12 pmid: 1 publication: Developmental Cell publication_identifier: eissn: - 1878-1551 issn: - 1534-5807 publication_status: published publisher: Cell Press quality_controlled: '1' researchdata_availability: upon request scopus_import: '1' status: public supplementarymaterial: yes title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 61 year: '2026' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold PlanS_conform: '1' _id: '21746' abstract: - lang: eng text: As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution. acknowledged_ssus: - _id: Bio - _id: LifeSc acknowledgement: 'We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).' article_number: '117227' article_processing_charge: Yes article_type: original author: - first_name: David full_name: Vijatovic, David id: cf391e77-ec3c-11ea-a124-d69323410b58 last_name: Vijatovic orcid: 0000-0002-5494-0941 - first_name: 'Florina Alexandra ' full_name: 'Toma, Florina Alexandra ' id: 2f73f876-f128-11eb-9611-b96b5a30cb0e last_name: Toma - first_name: Y full_name: Ignatyev, Y last_name: Ignatyev - first_name: Zoe P full_name: Harrington, Zoe P id: a8144562-32c9-11ee-b5ce-d9800628bda2 last_name: Harrington orcid: 0009-0008-0158-4032 - first_name: Christoph M full_name: Sommer, Christoph M id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87 last_name: Sommer orcid: 0000-0003-1216-9105 - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Matthijs Geert full_name: Smits, Matthijs Geert id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0 last_name: Smits - first_name: Marco full_name: Dalla Vecchia, Marco id: 02a7a869-ff06-11ed-a87f-86649d6077e5 last_name: Dalla Vecchia - first_name: Alexandra J. full_name: Trevisan, Alexandra J. last_name: Trevisan - first_name: Phillip full_name: Chapman, Phillip last_name: Chapman - first_name: Mara full_name: Julseth, Mara id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1 last_name: Julseth - first_name: Susan full_name: Brenner-Morton, Susan last_name: Brenner-Morton - first_name: Mariano I. full_name: Gabitto, Mariano I. last_name: Gabitto - first_name: Jeremy S. full_name: Dasen, Jeremy S. last_name: Dasen - first_name: Jay B. full_name: Bikoff, Jay B. last_name: Bikoff - first_name: Lora Beatrice Jaeger full_name: Sweeney, Lora Beatrice Jaeger id: 56BE8254-C4F0-11E9-8E45-0B23E6697425 last_name: Sweeney orcid: 0000-0001-9242-5601 citation: ama: Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 2026;45(4). doi:10.1016/j.celrep.2026.117227 apa: Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2026.117227 chicago: Vijatovic, David, Florina Alexandra Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell Reports. Cell Press, 2026. https://doi.org/10.1016/j.celrep.2026.117227. ieee: D. Vijatovic et al., “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” Cell Reports, vol. 45, no. 4. Cell Press, 2026. ista: Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227. mla: Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” Cell Reports, vol. 45, no. 4, 117227, Cell Press, 2026, doi:10.1016/j.celrep.2026.117227. short: D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026). corr_author: '1' date_created: 2026-04-19T22:07:43Z date_published: 2026-04-28T00:00:00Z date_updated: 2026-10-20T09:04:23Z day: '28' ddc: - '570' department: - _id: LoSw - _id: GradSch - _id: TiVo - _id: Bio - _id: NiBa doi: 10.1016/j.celrep.2026.117227 external_id: pmid: - '41964955 ' file: - access_level: open_access checksum: 0d26cdb5b8d8dec3a911d8261a65cdef content_type: application/pdf creator: dernst date_created: 2026-05-04T12:20:10Z date_updated: 2026-05-04T12:20:10Z file_id: '21795' file_name: 2026_CellReports_Vijatovic.pdf file_size: 14925958 relation: main_file success: 1 file_date_updated: 2026-05-04T12:20:10Z fulldoi: https://doi.org/10.1016/j.celrep.2026.117227 has_accepted_license: '1' intvolume: ' 45' issue: '4' language: - iso: eng month: '04' oa: 1 oa_version: Published Version pmid: 1 project: - _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae grant_number: '101041551' name: Development and Evolution of Tetrapod Motor Circuits - _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e grant_number: F7814 name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity' - _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473 grant_number: CZI01 name: Tools for automation and feedback microscopy - _id: bd73af52-d553-11ed-ba76-912049f0ac7a grant_number: FTI21-D-046 name: Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis - _id: cf428362-b037-11f1-b015-8277a8a2f63d grant_number: COE16 name: Neuronal circuits in health and disease (Sweeney) publication: Cell Reports publication_identifier: eissn: - 2211-1247 issn: - 2639-1856 publication_status: published publisher: Cell Press quality_controlled: '1' related_material: record: - id: '22667' relation: dissertation_contains status: public scopus_import: '1' status: public title: Multifold increase in spinal inhibitory cell types with emergence of limb movement tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 45 year: '2026' ... --- OA_place: repository OA_type: green _id: '20858' abstract: - lang: eng text: Targeted antigen delivery to immune cells, particularly dendritic cells, has emerged as a promising strategy to enhance therapeutic efficacy of vaccines, while minimizing adverse effects associated with conventional immunization. In this study, we use our previously described small glycomimetic molecule that is selectively recognized by the Langerhans cell (LC)-specific surface receptor Langerin and demonstrate specific delivery of protein antigens to these specialized dendritic cells. Our results show that Langerin-mediated antigen delivery significantly enhances the immune response in vivo, resulting in increased expansion and activation of antigen-specific T cells, compared to immunization with unmodified antigen. We demonstrate the feasibility of our LC-targeted platform for immune cell-specific immunization with protein antigen and underscore the potential of LCs as an access point for next-generation vaccines and immunotherapies. acknowledgement: This project was generously supported by Seedfinancing (grant no. P2282679) of the Austrian Bundesministerium für Digitalisierung und Wirtschaftsstandort and the Bundesministerium für Klimaschutz, Umwelt, Energie, Mobilität, Innovation, und Technologie, handled by the Austrian Wirtschaftsservice (aws), as well as by Life Science Call 2022 (grant no. FO999896442) of the Austrian Research Promotion Agency (FFG). We thank Mag. Michael Schunn from the PCF of the Institute of Science and Technology Austria for his continuous technical support. article_processing_charge: No article_type: original author: - first_name: Ramona full_name: Rica, Ramona last_name: Rica - first_name: Klara full_name: Klein, Klara last_name: Klein - first_name: Litty full_name: Johnson, Litty last_name: Johnson - first_name: Gabriele full_name: Carta, Gabriele last_name: Carta - first_name: Mirza full_name: Sarcevic, Mirza last_name: Sarcevic - first_name: Freyja full_name: Langer, Freyja id: 3C1BE782-F248-11E8-B48F-1D18A9856A87 last_name: Langer - first_name: Christoph full_name: Rademacher, Christoph last_name: Rademacher - first_name: Robert full_name: Wawrzinek, Robert last_name: Wawrzinek - first_name: Federica full_name: Quattrone, Federica last_name: Quattrone - first_name: Florian full_name: Sparber, Florian last_name: Sparber biorxivid: 1 citation: ama: Rica R, Klein K, Johnson L, et al. Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response. Molecular Therapy. 2026;34(1):397-406. doi:10.1016/j.ymthe.2025.10.008 apa: Rica, R., Klein, K., Johnson, L., Carta, G., Sarcevic, M., Langer, F., … Sparber, F. (2026). Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response. Molecular Therapy. Cell Press. https://doi.org/10.1016/j.ymthe.2025.10.008 chicago: Rica, Ramona, Klara Klein, Litty Johnson, Gabriele Carta, Mirza Sarcevic, Freyja Langer, Christoph Rademacher, Robert Wawrzinek, Federica Quattrone, and Florian Sparber. “Langerhans Cell-Targeted Protein Delivery Enhances Antigen-Specific Cellular Immune Response.” Molecular Therapy. Cell Press, 2026. https://doi.org/10.1016/j.ymthe.2025.10.008. ieee: R. Rica et al., “Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response,” Molecular Therapy, vol. 34, no. 1. Cell Press, pp. 397–406, 2026. ista: Rica R, Klein K, Johnson L, Carta G, Sarcevic M, Langer F, Rademacher C, Wawrzinek R, Quattrone F, Sparber F. 2026. Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response. Molecular Therapy. 34(1), 397–406. mla: Rica, Ramona, et al. “Langerhans Cell-Targeted Protein Delivery Enhances Antigen-Specific Cellular Immune Response.” Molecular Therapy, vol. 34, no. 1, Cell Press, 2026, pp. 397–406, doi:10.1016/j.ymthe.2025.10.008. short: R. Rica, K. Klein, L. Johnson, G. Carta, M. Sarcevic, F. Langer, C. Rademacher, R. Wawrzinek, F. Quattrone, F. Sparber, Molecular Therapy 34 (2026) 397–406. dataavailabilitystatement: The data that support the findings of this study are available on request from the corresponding authors. date_created: 2025-12-28T23:01:26Z date_published: 2026-01-07T00:00:00Z date_updated: 2026-10-20T09:03:08Z day: '07' department: - _id: PreCl doi: 10.1016/j.ymthe.2025.10.008 external_id: biorxivid: - 10.1101/2025.05.05.652195 fulldoi: https://doi.org/10.1016/j.ymthe.2025.10.008 intvolume: ' 34' issue: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1101/2025.05.05.652195 month: '01' oa: 1 oa_version: Preprint page: 397-406 publication: Molecular Therapy publication_identifier: eissn: - 1525-0024 issn: - 1525-0016 publication_status: published publisher: Cell Press quality_controlled: '1' researchdata_availability: upon request scopus_import: '1' status: public supplementarymaterial: yes title: Langerhans cell-targeted protein delivery enhances antigen-specific cellular immune response type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 34 year: '2026' ... --- OA_type: closed access _id: '20870' abstract: - lang: eng text: RNA sequencing (RNA-seq) methodologies have evolved rapidly, offering powerful tools to study gene expression, transcriptome dynamics, and molecular mechanisms in various biological contexts. However, the complexity of these approaches poses challenges in data interpretation, sensitivity, and applicability. This chapter provides a comprehensive overview of RNA-seq methodologies, highlighting their advantages, limitations, and applications, particularly in cardiovascular research. Bulk RNA sequencing enables high-throughput gene expression profiling but lacks the resolution to capture cellular heterogeneity and spatial context. Direct RNA sequencing preserves native RNA modifications, offering insights into post-transcriptional regulation, though it remains technically challenging. Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) bridge these gaps by resolving transcriptomic complexity at the cellular level and within tissue architecture, providing crucial insights into disease mechanisms such as atherosclerosis. By summarizing the strengths and limitations of these methodologies, this chapter aims to guide researchers in selecting the most suitable transcriptomic approach for their studies, ultimately advancing precision medicine and biomarker discovery in cardiovascular disease. article_processing_charge: No author: - first_name: Victoria full_name: Stopa, Victoria last_name: Stopa - first_name: Miron full_name: Sopić, Miron last_name: Sopić - first_name: Guanliang full_name: Li, Guanliang last_name: Li - first_name: Judith full_name: Sluimer, Judith last_name: Sluimer - first_name: José full_name: Basílio, José last_name: Basílio - first_name: Sander W. full_name: van der Laan, Sander W. last_name: van der Laan - first_name: David P. full_name: Kreil, David P. last_name: Kreil - first_name: Yvan full_name: Devaux, Yvan last_name: Devaux - first_name: Bernhard full_name: Hochreiter, Bernhard id: e6cab3de-17f6-11ed-9210-c1e42e045e9d last_name: Hochreiter citation: ama: 'Stopa V, Sopić M, Li G, et al. Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In: Devaux Y, Sopic M, eds. Transcriptomics in Atherosclerosis. Elsevier; 2025:131-172. doi:10.1016/b978-0-443-33064-3.00016-5' apa: 'Stopa, V., Sopić, M., Li, G., Sluimer, J., Basílio, J., van der Laan, S. W., … Hochreiter, B. (2025). Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In Y. Devaux & M. Sopic (Eds.), Transcriptomics in Atherosclerosis (pp. 131–172). Elsevier. https://doi.org/10.1016/b978-0-443-33064-3.00016-5' chicago: 'Stopa, Victoria, Miron Sopić, Guanliang Li, Judith Sluimer, José Basílio, Sander W. van der Laan, David P. Kreil, Yvan Devaux, and Bernhard Hochreiter. “Essentials of Transcriptomic Methods: Navigating through RNA Sequencing and Beyond.” In Transcriptomics in Atherosclerosis, edited by Yvan Devaux and Miron Sopic, 131–72. Elsevier, 2025. https://doi.org/10.1016/b978-0-443-33064-3.00016-5.' ieee: 'V. Stopa et al., “Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond,” in Transcriptomics in Atherosclerosis, Y. Devaux and M. Sopic, Eds. Elsevier, 2025, pp. 131–172.' ista: 'Stopa V, Sopić M, Li G, Sluimer J, Basílio J, van der Laan SW, Kreil DP, Devaux Y, Hochreiter B. 2025.Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond. In: Transcriptomics in Atherosclerosis. , 131–172.' mla: 'Stopa, Victoria, et al. “Essentials of Transcriptomic Methods: Navigating through RNA Sequencing and Beyond.” Transcriptomics in Atherosclerosis, edited by Yvan Devaux and Miron Sopic, Elsevier, 2025, pp. 131–72, doi:10.1016/b978-0-443-33064-3.00016-5.' short: V. Stopa, M. Sopić, G. Li, J. Sluimer, J. Basílio, S.W. van der Laan, D.P. Kreil, Y. Devaux, B. Hochreiter, in:, Y. Devaux, M. Sopic (Eds.), Transcriptomics in Atherosclerosis, Elsevier, 2025, pp. 131–172. date_created: 2025-12-29T12:16:22Z date_published: 2025-10-24T00:00:00Z date_updated: 2026-01-05T11:49:54Z day: '24' department: - _id: Bio doi: 10.1016/b978-0-443-33064-3.00016-5 editor: - first_name: Yvan full_name: Devaux, Yvan last_name: Devaux - first_name: Miron full_name: Sopic, Miron last_name: Sopic fulldoi: https://doi.org/10.1016/b978-0-443-33064-3.00016-5 language: - iso: eng month: '10' oa_version: None page: 131-172 publication: Transcriptomics in Atherosclerosis publication_identifier: isbn: - '9780443330643' publication_status: published publisher: Elsevier quality_controlled: '1' scopus_import: '1' status: public title: 'Essentials of transcriptomic methods: Navigating through RNA sequencing and beyond' type: book_chapter user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2025' ... --- OA_place: repository OA_type: green _id: '21920' abstract: - lang: eng text: 'Vertebrates display remarkable diversity of sensorimotor behaviors, each adapted to distinct ecological and survival demands. This diversity raises fundamental questions about the evolutionary origin of motor control: do conserved spinal circuits underlie these behaviors, and how have they diverged across species. Recent studies detail spinal cell-type architecture in mammals but comparable, high-resolution atlases of the non-mammalian spinal cord are lacking. Here, we compare spinal cord cell types between fish, frogs, mice and humans, spanning ∼450 million years of evolution. Across species, we define highly conserved programs of cell type specification that segregate spinal neurons into nearly identical cardinal classes during development. This contrasts with adult stages, when spinal cell-type composition selectively diverges for excitatory neuron subpopulations. Using spatial transcriptomics, we localize this species divergence to the superficial, dorsal spinal cord, where variant neuropeptide expression defines mammalian-specific cell types. The most dorsal spinal cord thus emerges as a recently evolved hub for sensory integration in mammals, a neospinal cord analogous to the neocortex.' acknowledgement: "We would like to thank the members of the Sweeney Lab for discussion and support; Andrey\r\nBydanov for technical assistance with single-cell sequencing processing; and Jay Bikoff,\r\nNikos Konstantinides, Maria Tosches, and Graziana Gatto for comments on the manuscript. \r\nThis research was supported by: Horizon Europe ERC Starting Grant 101041551 (L.B.S,\r\nY.I., S.P.); Special Research Program (SFB) of the Austrian Science Fund (FWF) F7814-B\r\n(L.B.S., S.P., E.M.T); Austrian Science Fund (FWF) 10.55776/COE16 (L.B.S., Y.I., E.M.T.);\r\nAustrian Academy of Sciences DOC Fellowship 27229 (S.P.); ERC Advanced Grant 742046\r\n(E.M.T.); NIH award R24 OD031956 (L.P.); and in part by the Intramural Research\r\nProgram of the National Institutes of Health (NIH) through 1ZIA NS003153 to A.J.L.\r\nThe contributions of the NIH author are considered Works of the United States\r\nGovernment. The findings and conclusions presented in this paper are those of\r\nthe authors and do not necessarily reflect the views of the NIH or the U.S. Department\r\nof Health and Human Services. " article_processing_charge: No author: - first_name: Yuri full_name: Ignatyev, Yuri last_name: Ignatyev - first_name: Stavros full_name: Papadopoulos, Stavros id: 40606b92-f128-11eb-9611-bf66a98cfa5c last_name: Papadopoulos - first_name: Mateja full_name: Soretić, Mateja last_name: Soretić - first_name: Jake full_name: Yeung, Jake id: 123012b2-db30-11eb-b4d8-a35840c0551b last_name: Yeung orcid: 0000-0003-1732-1559 - first_name: Tzi-Yang full_name: Lin, Tzi-Yang last_name: Lin - first_name: Elly M full_name: Tanaka, Elly M last_name: Tanaka - first_name: Leonid full_name: Peshkin, Leonid last_name: Peshkin - first_name: Ariel J full_name: Levine, Ariel J last_name: Levine - first_name: Mariano I full_name: Gabitto, Mariano I last_name: Gabitto - first_name: Lora Beatrice Jaeger full_name: Sweeney, Lora Beatrice Jaeger id: 56BE8254-C4F0-11E9-8E45-0B23E6697425 last_name: Sweeney orcid: 0000-0001-9242-5601 citation: ama: Ignatyev Y, Papadopoulos S, Soretić M, et al. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv. doi:10.1101/2025.10.09.680955 apa: Ignatyev, Y., Papadopoulos, S., Soretić, M., Yeung, J., Lin, T.-Y., Tanaka, E. M., … Sweeney, L. B. (n.d.). Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv. https://doi.org/10.1101/2025.10.09.680955 chicago: Ignatyev, Yuri, Stavros Papadopoulos, Mateja Soretić, Jake Yeung, Tzi-Yang Lin, Elly M Tanaka, Leonid Peshkin, Ariel J Levine, Mariano I Gabitto, and Lora B. Sweeney. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” BioRxiv, n.d. https://doi.org/10.1101/2025.10.09.680955. ieee: Y. Ignatyev et al., “Innovations in spinal cord cell type heterogeneity across vertebrate evolution,” bioRxiv. . ista: Ignatyev Y, Papadopoulos S, Soretić M, Yeung J, Lin T-Y, Tanaka EM, Peshkin L, Levine AJ, Gabitto MI, Sweeney LB. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv, 10.1101/2025.10.09.680955. mla: Ignatyev, Yuri, et al. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” BioRxiv, doi:10.1101/2025.10.09.680955. short: Y. Ignatyev, S. Papadopoulos, M. Soretić, J. Yeung, T.-Y. Lin, E.M. Tanaka, L. Peshkin, A.J. Levine, M.I. Gabitto, L.B. Sweeney, BioRxiv (n.d.). corr_author: '1' date_created: 2026-05-27T06:54:04Z date_published: 2025-10-11T00:00:00Z date_updated: 2026-05-27T07:25:41Z day: '11' department: - _id: LoSw - _id: ScienComp doi: 10.1101/2025.10.09.680955 fulldoi: https://doi.org/10.1101/2025.10.09.680955 language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1101/2025.10.09.680955 month: '10' oa: 1 oa_version: Preprint project: - _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae grant_number: '101041551' name: Development and Evolution of Tetrapod Motor Circuits - _id: 907b765e-16d5-11f0-9cad-fef108a945b1 grant_number: '27229' name: 'A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb transition of Xenopus metamorphosis' publication: bioRxiv publication_status: submitted status: public title: Innovations in spinal cord cell type heterogeneity across vertebrate evolution tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: preprint user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 year: '2025' ... --- OA_place: publisher OA_type: hybrid _id: '19076' abstract: - lang: eng text: For accurate perception and motor control, an animal must distinguish between sensory experiences elicited by external stimuli and those elicited by its own actions. The diversity of behaviors and their complex influences on the senses make this distinction challenging. Here, we uncover an action–cue hub that coordinates motor commands with visual processing in the brain’s first visual relay. We show that the ventral lateral geniculate nucleus (vLGN) acts as a corollary discharge center, integrating visual translational optic flow signals with motor copies from saccades, locomotion and pupil dynamics. The vLGN relays these signals to correct action-specific visual distortions and to refine perception, as shown for the superior colliculus and in a depth-estimation task. Simultaneously, brain-wide vLGN projections drive corrective actions necessary for accurate visuomotor control. Our results reveal an extended corollary discharge architecture that refines early visual transformations and coordinates actions via a distributed hub-and-spoke network to enable visual perception during action. acknowledged_ssus: - _id: ScienComp - _id: PreCl - _id: LifeSc - _id: Bio acknowledgement: We thank Y. Ben-Simon for generously making viral vectors for retrograde tracing available, as well as J. Watson and F. Marr for reagents. We also thank R. Shigemoto, W. Młynarski and members of the Neuroethology group for their comments on the manuscript and L. Burnett for her schematic drawings. This research was supported by the Scientific Service Units of ISTA through resources provided by Scientific Computing, the Preclinical Facility, the Lab Support Facility and the Imaging and Optics Facility, in particular F. Lange, M. Schunn and T. Asenov. This work was supported by European Research Council Starting Grant no. 756502 (M.J.) and European Research Council Consolidator Grant no. 101086580 (M.J.); and EMBO ALTF grant no. 1098-2017 (A.S.) and Human Frontiers Science Program grant no. LT000256/2018-L (A.S.). Open access funding provided by Institute of Science and Technology (IST Austria). article_number: '7278' article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Tomas A full_name: Vega Zuniga, Tomas A id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87 last_name: Vega Zuniga - first_name: Anton L full_name: Sumser, Anton L id: 3320A096-F248-11E8-B48F-1D18A9856A87 last_name: Sumser orcid: 0000-0002-4792-1881 - first_name: Olga full_name: Symonova, Olga id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87 last_name: Symonova orcid: 0000-0003-2012-9947 - first_name: Peter full_name: Koppensteiner, Peter id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87 last_name: Koppensteiner orcid: 0000-0002-3509-1948 - first_name: Florian full_name: Schmidt, Florian id: A2EF226A-AF19-11E9-924C-0525E6697425 last_name: Schmidt - first_name: Maximilian A full_name: Jösch, Maximilian A id: 2BD278E6-F248-11E8-B48F-1D18A9856A87 last_name: Jösch orcid: 0000-0002-3937-1330 citation: ama: Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA. A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics. Nature Neuroscience. 2025;28. doi:10.1038/s41593-025-01874-w apa: Vega Zuniga, T. A., Sumser, A. L., Symonova, O., Koppensteiner, P., Schmidt, F., & Jösch, M. A. (2025). A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics. Nature Neuroscience. Springer Nature. https://doi.org/10.1038/s41593-025-01874-w chicago: Vega Zuniga, Tomas A, Anton L Sumser, Olga Symonova, Peter Koppensteiner, Florian Schmidt, and Maximilian A Jösch. “A Thalamic Hub-and-Spoke Network Enables Visual Perception during Action by Coordinating Visuomotor Dynamics.” Nature Neuroscience. Springer Nature, 2025. https://doi.org/10.1038/s41593-025-01874-w. ieee: T. A. Vega Zuniga, A. L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt, and M. A. Jösch, “A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics,” Nature Neuroscience, vol. 28. Springer Nature, 2025. ista: Vega Zuniga TA, Sumser AL, Symonova O, Koppensteiner P, Schmidt F, Jösch MA. 2025. A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics. Nature Neuroscience. 28, 7278. mla: Vega Zuniga, Tomas A., et al. “A Thalamic Hub-and-Spoke Network Enables Visual Perception during Action by Coordinating Visuomotor Dynamics.” Nature Neuroscience, vol. 28, 7278, Springer Nature, 2025, doi:10.1038/s41593-025-01874-w. short: T.A. Vega Zuniga, A.L. Sumser, O. Symonova, P. Koppensteiner, F. Schmidt, M.A. Jösch, Nature Neuroscience 28 (2025). corr_author: '1' date_created: 2025-02-23T23:01:58Z date_published: 2025-03-01T00:00:00Z date_updated: 2026-06-18T18:12:08Z day: '01' ddc: - '570' department: - _id: MaJö - _id: PreCl doi: 10.1038/s41593-025-01874-w ec_funded: 1 external_id: isi: - '001416866800001' pmid: - '39930095' fulldoi: https://doi.org/10.1038/s41593-025-01874-w has_accepted_license: '1' intvolume: ' 28' isi: 1 language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1038/s41593-025-01874-w month: '03' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 2634E9D2-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '756502' name: Circuits of Visual Attention - _id: bdaf81a8-d553-11ed-ba76-c95961984540 grant_number: '101086580' name: 'Action Selection in the Midbrain: Neuromodulation of Visuomotor Senses' - _id: 264FEA02-B435-11E9-9278-68D0E5697425 grant_number: ALTF 1098-2017 name: Connecting sensory with motor processing in the superior colliculus - _id: 266D407A-B435-11E9-9278-68D0E5697425 grant_number: LT000256 name: Neuronal networks of salience and spatial detection in the murine superior colliculus publication: Nature Neuroscience publication_identifier: eissn: - 1546-1726 issn: - 1097-6256 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: link: - description: News on ISTA Website relation: press_release url: https://ista.ac.at/en/news/high-tech-video-optimization-in-our-brain/ record: - id: '18579' relation: research_data status: public scopus_import: '1' status: public title: A thalamic hub-and-spoke network enables visual perception during action by coordinating visuomotor dynamics tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 28 year: '2025' ... --- OA_place: repository OA_type: gold _id: '19294' abstract: - lang: eng text: Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of non-functional “promoter leakiness”, merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the non-canonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g. control of costly-to-induce multi-drug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multi-drug resistance is crucial for effective public health measures. article_processing_charge: No author: - first_name: Kirti full_name: Jain, Kirti id: 330F0278-F248-11E8-B48F-1D18A9856A87 last_name: Jain orcid: 0000-0002-3809-0449 - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Olga full_name: Bochkareva, Olga id: C4558D3C-6102-11E9-A62E-F418E6697425 last_name: Bochkareva orcid: 0000-0003-1006-6639 - first_name: Roderich full_name: Römhild, Roderich id: 68E56E44-62B0-11EA-B963-444F3DDC885E last_name: Römhild orcid: 0000-0001-9480-5261 - first_name: Gašper full_name: Tkačik, Gašper id: 3D494DCA-F248-11E8-B48F-1D18A9856A87 last_name: Tkačik orcid: 0000-0002-6699-1455 - first_name: Calin C full_name: Guet, Calin C id: 47F8433E-F248-11E8-B48F-1D18A9856A87 last_name: Guet orcid: 0000-0001-6220-2052 citation: ama: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Data for “Pulsatile basal gene expression as a fitness determinant in bacteria.” 2025. doi:10.15479/AT:ISTA:19294 apa: Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., & Guet, C. C. (2025). Data for “Pulsatile basal gene expression as a fitness determinant in bacteria.” Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:19294 chicago: Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Data for ‘Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.’” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT:ISTA:19294. ieee: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria.’” Institute of Science and Technology Austria, 2025. ista: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Data for ‘Pulsatile basal gene expression as a fitness determinant in bacteria’, Institute of Science and Technology Austria, 10.15479/AT:ISTA:19294. mla: Jain, Kirti, et al. Data for “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” Institute of Science and Technology Austria, 2025, doi:10.15479/AT:ISTA:19294. short: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, (2025). corr_author: '1' date_created: 2025-03-04T13:27:21Z date_published: 2025-03-04T00:00:00Z date_updated: 2026-05-20T08:33:07Z day: '04' ddc: - '570' department: - _id: CaGu - _id: Bio - _id: FyKo - _id: GaTk doi: 10.15479/AT:ISTA:19294 file: - access_level: open_access checksum: 11a5bab307a4e1e1598a1577d8a2fbb5 content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet creator: dernst date_created: 2025-03-04T13:08:52Z date_updated: 2025-03-04T13:08:52Z file_id: '19295' file_name: Data1.xlsx file_size: 269054 relation: main_file success: 1 - access_level: open_access checksum: 3b057894322639f0c1e11fb2e84173e6 content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet creator: dernst date_created: 2025-03-04T13:08:52Z date_updated: 2025-03-04T13:08:52Z file_id: '19296' file_name: Data2.xlsx file_size: 87143 relation: main_file success: 1 - access_level: open_access checksum: a551e1b79a138bb97ab96979aa475b3c content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet creator: dernst date_created: 2025-03-04T13:08:52Z date_updated: 2025-03-04T13:08:52Z file_id: '19297' file_name: Data3.xlsx file_size: 129101 relation: main_file success: 1 - access_level: open_access checksum: d6909c9bf111f859058082b1a2f970c4 content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet creator: dernst date_created: 2025-03-04T13:08:52Z date_updated: 2025-03-04T13:08:52Z file_id: '19298' file_name: Data4.xlsx file_size: 86243 relation: main_file success: 1 - access_level: open_access checksum: e5725a3a118a3f06846104906c8792c7 content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet creator: dernst date_created: 2025-03-04T13:08:52Z date_updated: 2025-03-04T13:08:52Z file_id: '19299' file_name: Data5.xlsx file_size: 26049 relation: main_file success: 1 - access_level: open_access checksum: 16763c127049f14bd587dc885677dce1 content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet creator: dernst date_created: 2025-03-04T13:08:52Z date_updated: 2025-03-04T13:08:52Z file_id: '19300' file_name: RawData_2_3.xlsx file_size: 7327253 relation: main_file success: 1 - access_level: open_access checksum: 2f3e1a368b4e3abc46bf37e02724f0f4 content_type: text/plain creator: dernst date_created: 2025-03-05T07:39:38Z date_updated: 2025-03-05T07:39:38Z file_id: '19301' file_name: Readme.txt file_size: 606 relation: main_file success: 1 file_date_updated: 2025-03-05T07:39:38Z fulldoi: https://doi.org/10.15479/AT:ISTA:19294 has_accepted_license: '1' month: '03' oa: 1 oa_version: Published Version publisher: Institute of Science and Technology Austria related_material: record: - id: '19626' relation: used_in_publication status: public status: public title: Data for "Pulsatile basal gene expression as a fitness determinant in bacteria" tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: research_data user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2025' ... --- APC_amount: 5949 EUR OA_place: publisher OA_type: hybrid _id: '19626' abstract: - lang: eng text: Active regulation of gene expression, orchestrated by complex interactions of activators and repressors at promoters, controls the fate of organisms. In contrast, basal expression at uninduced promoters is considered to be a dynamically inert mode of nonfunctional “promoter leakiness,” merely a byproduct of transcriptional regulation. Here, we investigate the basal expression mode of the mar operon, the main regulator of intrinsic multiple antibiotic resistance in Escherichia coli, and link its dynamic properties to the noncanonical, yet highly conserved start codon of marR across Enterobacteriaceae. Real-time, single-cell measurements across tens of generations reveal that basal expression consists of rare stochastic gene expression pulses, which maximize variability in wildtype and, surprisingly, transiently accelerate cellular elongation rates. Competition experiments show that basal expression confers fitness advantages to wildtype across several transitions between exponential and stationary growth by shortening lag times. The dynamically rich basal expression of the mar operon has likely been evolutionarily maintained for its role in growth homeostasis of Enterobacteria within the gut environment, thereby allowing other ancillary gene regulatory roles to evolve, e.g., control of costly-to-induce multidrug efflux pumps. Understanding the complex selection forces governing genetic systems involved in intrinsic multidrug resistance is crucial for effective public health measures. acknowledged_ssus: - _id: Bio acknowledgement: K.J. thanks B. Wu, I. Tomanek, K. Tomasek for detailed discussions on the manuscript, all other members from the Guet laboratory for valuable feedback, R. Chait, & Imaging and Optics Facility, Institute of Science and Technology Austria for helping with microscopy, Dr. Sudha Rao and Dr. Raja Mugasimangalam, Genotypic Technology India for allowing time off to address the revisions. K.J. acknowledges Institute of Science and Technology fellowship IC1006FELL02, R.H. was supported in part by Chan Zuckerberg Initiative and Donor Advised-Fund grant 2020-225401 (https://doi.org/10.37921/120055ratwvi), O.O.B. acknowledges Fonds Zur Förderung der Wissenschaftlichen Forschung (FWF) Grant ESP253-B, R.R. acknowledges FWF Grant 10.55776/ESP219, C.C.G. acknowledges FWF I5127-B. article_number: e2413709122 article_processing_charge: Yes (in subscription journal) article_type: original author: - first_name: Kirti full_name: Jain, Kirti id: 330F0278-F248-11E8-B48F-1D18A9856A87 last_name: Jain orcid: 0000-0002-3809-0449 - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Olga full_name: Bochkareva, Olga id: C4558D3C-6102-11E9-A62E-F418E6697425 last_name: Bochkareva orcid: 0000-0003-1006-6639 - first_name: Roderich full_name: Römhild, Roderich id: 68E56E44-62B0-11EA-B963-444F3DDC885E last_name: Römhild orcid: 0000-0001-9480-5261 - first_name: Gašper full_name: Tkačik, Gašper id: 3D494DCA-F248-11E8-B48F-1D18A9856A87 last_name: Tkačik orcid: 0000-0002-6699-1455 - first_name: Calin C full_name: Guet, Calin C id: 47F8433E-F248-11E8-B48F-1D18A9856A87 last_name: Guet orcid: 0000-0001-6220-2052 citation: ama: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. Pulsatile basal gene expression as a fitness determinant in bacteria. Proceedings of the National Academy of Sciences. 2025;122(15). doi:10.1073/pnas.2413709122 apa: Jain, K., Hauschild, R., Bochkareva, O., Römhild, R., Tkačik, G., & Guet, C. C. (2025). Pulsatile basal gene expression as a fitness determinant in bacteria. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2413709122 chicago: Jain, Kirti, Robert Hauschild, Olga Bochkareva, Roderich Römhild, Gašper Tkačik, and Calin C Guet. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2413709122. ieee: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, and C. C. Guet, “Pulsatile basal gene expression as a fitness determinant in bacteria,” Proceedings of the National Academy of Sciences, vol. 122, no. 15. National Academy of Sciences, 2025. ista: Jain K, Hauschild R, Bochkareva O, Römhild R, Tkačik G, Guet CC. 2025. Pulsatile basal gene expression as a fitness determinant in bacteria. Proceedings of the National Academy of Sciences. 122(15), e2413709122. mla: Jain, Kirti, et al. “Pulsatile Basal Gene Expression as a Fitness Determinant in Bacteria.” Proceedings of the National Academy of Sciences, vol. 122, no. 15, e2413709122, National Academy of Sciences, 2025, doi:10.1073/pnas.2413709122. short: K. Jain, R. Hauschild, O. Bochkareva, R. Römhild, G. Tkačik, C.C. Guet, Proceedings of the National Academy of Sciences 122 (2025). corr_author: '1' date_created: 2025-04-27T22:02:13Z date_published: 2025-04-15T00:00:00Z date_updated: 2026-05-20T08:33:08Z day: '15' ddc: - '570' department: - _id: CaGu - _id: Bio - _id: FyKo - _id: GaTk doi: 10.1073/pnas.2413709122 external_id: isi: - '001471235200001' pmid: - '40193613' file: - access_level: open_access checksum: 115a687f40009660eb4b38b4f6559d41 content_type: application/pdf creator: dernst date_created: 2025-06-24T07:27:43Z date_updated: 2025-06-24T07:27:43Z file_id: '19888' file_name: 2025_PNAS_Jain.pdf file_size: 2949523 relation: main_file success: 1 file_date_updated: 2025-06-24T07:27:43Z fulldoi: https://doi.org/10.1073/pnas.2413709122 has_accepted_license: '1' intvolume: ' 122' isi: 1 issue: '15' language: - iso: eng month: '04' oa: 1 oa_version: Published Version pmid: 1 project: - _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473 grant_number: CZI01 name: Tools for automation and feedback microscopy - _id: bd6f94d1-d553-11ed-ba76-ae9f07250f74 grant_number: E219 name: Non-canonical antibiotic interactions - _id: 34e076d6-11ca-11ed-8bc3-aec76c41a181 grant_number: I05127 name: Evolutionary analysis of gene regulation publication: Proceedings of the National Academy of Sciences publication_identifier: eissn: - 1091-6490 issn: - 0027-8424 publication_status: published publisher: National Academy of Sciences quality_controlled: '1' related_material: link: - description: News on ISTA website relation: press_release url: https://ista.ac.at/en/news/clockwork-just-for-antibiotic-resistance/ record: - id: '19294' relation: research_data status: public scopus_import: '1' status: public title: Pulsatile basal gene expression as a fitness determinant in bacteria tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 122 year: '2025' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold _id: '19663' abstract: - lang: eng text: The centrosome is a microtubule orchestrator, nucleating and anchoring microtubules that grow radially and exert forces on cargos. At the same time, mechanical stresses from the microenvironment and cellular shape changes compress and bend microtubules. Yet, centrosomes are membraneless organelles, raising the question of how centrosomes withstand mechanical forces. Here, we discover that centrosomes can deform and even fracture. We reveal that centrosomes experience deformations during navigational pathfinding within motile cells. Coherence of the centrosome is maintained by Dyrk3 and cNAP1, preventing fracturing by forces. While cells can compensate for the depletion of centriolar-based centrosomes, the fracturing of centrosomes impedes cellular function by generating coexisting microtubule organizing centers that compete during path navigation and thereby cause cellular entanglement in the microenvironment. Our findings show that cells actively maintain the integrity of the centrosome to withstand mechanical forces. These results suggest that centrosome stability preservation is fundamental, given that almost all cells in multicellular organisms experience forces. acknowledgement: "We thank L. Pelkmans and D. Dormann for providing Dyrk3-EGFP plasmids; M. Heuzé for providing a RFP-Pericentrin plasmid; T. Balla for providing a PH-Akt-GFP plasmid; E. Snaar-Jagalska for providing a pLenti-V6.3 Ultra-Chili plasmid; T. Tang for providing CEP120 a plasmid; D. Trono for providing pMD2.G and psSPAX2 plasmids; M. Sixt for providing EB3-mCherry and EMTB-mCherry plasmids as well as 3T3 fibroblasts, Lifeact-GFP Hoxb8 cells, and LX293 cells; M. Duggan for RNA isolation from migrating DCs; M. Schuster from the Biomedical Sequencing Facility at CeMM; J. Schwarz for providing Jurkat T cells; M. Götz for initial transcriptome analysis; M. Götz and F. Merino for discussion and sharing reagents; F. Gärtner for discussions and support; M. Benjamin Braun for critical reading of the manuscript; and the Core Facility Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the Biomedical Center (BMC) for excellent support.\r\nThis work was supported by Peter Hans Hofschneider Professorship of the Stiftung Experimentelle Biomedizin (J.R.); German Research Foundation grant “CRC914, project A12” (J.R); German Research Foundation grant “SPP2332, project 492014049” (J.R.); LMU Institutional Strategy LMU-Excellent within the framework of the German Excellence Initiative (J.R.); Medical & Clinician Scientist Program (MCSP) LMU Munich (J.K.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) under Germany’s Excellence Strategy – EXC2151 – 390873048 (D.B.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) Grossgeräteantrag 457838313 and under Germany’s Excellence Strategy – EXC 2151 – 390873048 (E.K.); Ministry of Innovation, Science and Research of North-Rhine-Westphalia (fellowship AZ: 421-8.03.03.02-137069) (E.K.); TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments (E.K.); and CZI grant DAF2020-225401 and grant (DOI https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF (R.H.)." article_number: eadx4047 article_processing_charge: Yes article_type: original author: - first_name: Madeleine T. full_name: Schmitt, Madeleine T. last_name: Schmitt - first_name: Janina full_name: Kroll, Janina last_name: Kroll - first_name: Mauricio J.A. full_name: Ruiz-Fernandez, Mauricio J.A. last_name: Ruiz-Fernandez - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Shaunak full_name: Ghosh, Shaunak last_name: Ghosh - first_name: Petra full_name: Kameritsch, Petra last_name: Kameritsch - first_name: Jack full_name: Merrin, Jack id: 4515C308-F248-11E8-B48F-1D18A9856A87 last_name: Merrin orcid: 0000-0001-5145-4609 - first_name: Johanna full_name: Schmid, Johanna last_name: Schmid - first_name: Kasia full_name: Stefanowski, Kasia last_name: Stefanowski - first_name: Andreas W. full_name: Thomae, Andreas W. last_name: Thomae - first_name: Jingyuan full_name: Cheng, Jingyuan last_name: Cheng - first_name: Gamze Naz full_name: Öztan, Gamze Naz last_name: Öztan - first_name: Peter full_name: Konopka, Peter last_name: Konopka - first_name: Germán Camargo full_name: Ortega, Germán Camargo last_name: Ortega - first_name: Thomas full_name: Penz, Thomas last_name: Penz - first_name: Luisa full_name: Bach, Luisa last_name: Bach - first_name: Dirk full_name: Baumjohann, Dirk last_name: Baumjohann - first_name: Christoph full_name: Bock, Christoph last_name: Bock - first_name: Tobias full_name: Straub, Tobias last_name: Straub - first_name: Felix full_name: Meissner, Felix last_name: Meissner - first_name: Eva full_name: Kiermaier, Eva id: 3EB04B78-F248-11E8-B48F-1D18A9856A87 last_name: Kiermaier orcid: 0000-0001-6165-5738 - first_name: Jörg full_name: Renkawitz, Jörg id: 3F0587C8-F248-11E8-B48F-1D18A9856A87 last_name: Renkawitz orcid: 0000-0003-2856-3369 citation: ama: Schmitt MT, Kroll J, Ruiz-Fernandez MJA, et al. Protecting centrosomes from fracturing enables efficient cell navigation. Science Advances. 2025;11(17). doi:10.1126/sciadv.adx4047 apa: Schmitt, M. T., Kroll, J., Ruiz-Fernandez, M. J. A., Hauschild, R., Ghosh, S., Kameritsch, P., … Renkawitz, J. (2025). Protecting centrosomes from fracturing enables efficient cell navigation. Science Advances. AAAS. https://doi.org/10.1126/sciadv.adx4047 chicago: Schmitt, Madeleine T., Janina Kroll, Mauricio J.A. Ruiz-Fernandez, Robert Hauschild, Shaunak Ghosh, Petra Kameritsch, Jack Merrin, et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” Science Advances. AAAS, 2025. https://doi.org/10.1126/sciadv.adx4047. ieee: M. T. Schmitt et al., “Protecting centrosomes from fracturing enables efficient cell navigation,” Science Advances, vol. 11, no. 17. AAAS, 2025. ista: Schmitt MT, Kroll J, Ruiz-Fernandez MJA, Hauschild R, Ghosh S, Kameritsch P, Merrin J, Schmid J, Stefanowski K, Thomae AW, Cheng J, Öztan GN, Konopka P, Ortega GC, Penz T, Bach L, Baumjohann D, Bock C, Straub T, Meissner F, Kiermaier E, Renkawitz J. 2025. Protecting centrosomes from fracturing enables efficient cell navigation. Science Advances. 11(17), eadx4047. mla: Schmitt, Madeleine T., et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” Science Advances, vol. 11, no. 17, eadx4047, AAAS, 2025, doi:10.1126/sciadv.adx4047. short: M.T. Schmitt, J. Kroll, M.J.A. Ruiz-Fernandez, R. Hauschild, S. Ghosh, P. Kameritsch, J. Merrin, J. Schmid, K. Stefanowski, A.W. Thomae, J. Cheng, G.N. Öztan, P. Konopka, G.C. Ortega, T. Penz, L. Bach, D. Baumjohann, C. Bock, T. Straub, F. Meissner, E. Kiermaier, J. Renkawitz, Science Advances 11 (2025). date_created: 2025-05-11T22:02:38Z date_published: 2025-04-25T00:00:00Z date_updated: 2025-09-30T12:26:21Z day: '25' ddc: - '570' department: - _id: Bio - _id: NanoFab doi: 10.1126/sciadv.adx4047 external_id: isi: - '001476113400016' pmid: - '40279414' file: - access_level: open_access checksum: e8ba22922fa5b23ccfcce8865f57226c content_type: application/pdf creator: dernst date_created: 2025-05-12T07:46:10Z date_updated: 2025-05-12T07:46:10Z file_id: '19679' file_name: 2025_ScienceAdvance_Schmitt.pdf file_size: 2707050 relation: main_file success: 1 file_date_updated: 2025-05-12T07:46:10Z fulldoi: https://doi.org/10.1126/sciadv.adx4047 has_accepted_license: '1' intvolume: ' 11' isi: 1 issue: '17' language: - iso: eng month: '04' oa: 1 oa_version: Published Version pmid: 1 project: - _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473 grant_number: CZI01 name: Tools for automation and feedback microscopy publication: Science Advances publication_identifier: eissn: - 2375-2548 publication_status: published publisher: AAAS quality_controlled: '1' scopus_import: '1' status: public title: Protecting centrosomes from fracturing enables efficient cell navigation tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 11 year: '2025' ... --- OA_type: closed access _id: '20055' abstract: - lang: eng text: Supercrystals represent three-dimensional orderings of colloidal nanocrystals (NCs), showcasing collective properties in photonics, phononics, and electronics applications.1,2 Recent studies have shown that such assemblies are directly produced during nanocrystal reactions.3–6 However, a fundamental understanding of in situ formed supercrystals that withstand typical NC purification processes remains underexplored, which is important for further use. Herein, we report the reaction precursor-mediated formation of stable PbTe supercrystals. Rationalizing the formation of these assemblies through small-angle x-ray scattering (SAXS) measurements, we unveil their formation mechanism. Our findings reveal that the supercrystal formation occurs in the presence of an excess of lead oleates in the crude solution. It should be noted that the formed supercrystals can be stabilized under specific conditions determined by the lead oleate cluster concentration, content of trioctylphosphine telluride (TOP-Te), NC size and the need of an annealing step at mild conditions. Furthermore, this approach allows for the continuous growth of a secondary phase within the supercrystal; for example in the case of PbTe supercrystals, a PbS shell can be grown on each PbTe NC constituent, resulting in core-shell PbTe-PbS supercrystals. Our work elucidates that reaction precursors play an important role in in situ SC formation and stabilization, implying the possibility of applying this knowledge to other NC reactions. acknowledged_ssus: - _id: EM-Fac - _id: NMR - _id: LifeSc acknowledgement: ISTA and the Werner Siemens Foundation financially supported this work. The Scientific Service Units (SSU) of ISTA supported this research through resources provided by the Electron Microscopy Facility (EMF), NMR Facility and the Lab Support Facility (LSF). article_number: '173' article_processing_charge: No author: - first_name: Seungho full_name: Lee, Seungho id: BB243B88-D767-11E9-B658-BC13E6697425 last_name: Lee orcid: 0000-0002-6962-8598 - first_name: Daniel full_name: Balazs, Daniel id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E last_name: Balazs orcid: 0000-0001-7597-043X - first_name: Sharona full_name: Horta, Sharona id: 03a7e858-01b1-11ec-8b71-99ae6c4a05bc last_name: Horta - first_name: Aiswarya full_name: Rayaroth Puthiyaveettil, Aiswarya id: 8aceb01b-8972-11ed-ae7b-d5fe53775add last_name: Rayaroth Puthiyaveettil - first_name: Maria full_name: Ibáñez, Maria id: 43C61214-F248-11E8-B48F-1D18A9856A87 last_name: Ibáñez orcid: 0000-0001-5013-2843 citation: ama: 'Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. In: Proceedings of the MATSUS Spring 2025 Conference. Fundació de la comunitat valenciana SCITO; 2025. doi:10.29363/nanoge.matsusspring.2025.173' apa: 'Lee, S., Balazs, D., Horta, S., Rayaroth Puthiyaveettil, A., & Ibáñez, M. (2025). Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. In Proceedings of the MATSUS Spring 2025 Conference. Sevilla, Spain: Fundació de la comunitat valenciana SCITO. https://doi.org/10.29363/nanoge.matsusspring.2025.173' chicago: 'Lee, Seungho, Daniel Balazs, Sharona Horta, Aiswarya Rayaroth Puthiyaveettil, and Maria Ibáñez. “Reaction Precursor-Mediated Formation of Stable Supercrystals in Colloidal Nanocrystal Synthesis: PbTe Case.” In Proceedings of the MATSUS Spring 2025 Conference. Fundació de la comunitat valenciana SCITO, 2025. https://doi.org/10.29363/nanoge.matsusspring.2025.173.' ieee: 'S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, and M. Ibáñez, “Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case,” in Proceedings of the MATSUS Spring 2025 Conference, Sevilla, Spain, 2025.' ista: 'Lee S, Balazs D, Horta S, Rayaroth Puthiyaveettil A, Ibáñez M. 2025. Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case. Proceedings of the MATSUS Spring 2025 Conference. MATSUS: Materials for Sustainable Development Conference, 173.' mla: 'Lee, Seungho, et al. “Reaction Precursor-Mediated Formation of Stable Supercrystals in Colloidal Nanocrystal Synthesis: PbTe Case.” Proceedings of the MATSUS Spring 2025 Conference, 173, Fundació de la comunitat valenciana SCITO, 2025, doi:10.29363/nanoge.matsusspring.2025.173.' short: S. Lee, D. Balazs, S. Horta, A. Rayaroth Puthiyaveettil, M. Ibáñez, in:, Proceedings of the MATSUS Spring 2025 Conference, Fundació de la comunitat valenciana SCITO, 2025. conference: end_date: 2025-03-07 location: Sevilla, Spain name: 'MATSUS: Materials for Sustainable Development Conference' start_date: 2025-03-03 corr_author: '1' date_created: 2025-07-21T08:33:20Z date_published: 2025-03-15T00:00:00Z date_updated: 2026-02-19T09:25:57Z day: '15' department: - _id: MaIb - _id: LifeSc doi: 10.29363/nanoge.matsusspring.2025.173 fulldoi: https://doi.org/10.29363/nanoge.matsusspring.2025.173 language: - iso: eng month: '03' oa_version: None project: - _id: 9B8F7476-BA93-11EA-9121-9846C619BF3A name: 'HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery' publication: Proceedings of the MATSUS Spring 2025 Conference publication_status: published publisher: Fundació de la comunitat valenciana SCITO quality_controlled: '1' status: public title: 'Reaction precursor-mediated formation of stable supercrystals in colloidal nanocrystal synthesis: PbTe case' type: conference user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2025' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '20082' abstract: - lang: eng text: Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence. acknowledged_ssus: - _id: Bio - _id: LifeSc acknowledgement: This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria). article_processing_charge: Yes (via OA deal) article_type: letter_note author: - first_name: Patricia full_name: Dos Reis Rodrigues, Patricia id: 26E95904-5160-11E9-9C0B-C5B0DC97E90F last_name: Dos Reis Rodrigues orcid: 0000-0003-1681-508X - first_name: Mario full_name: Avellaneda Sarrió, Mario id: DC4BA84C-56E6-11EA-AD5D-348C3DDC885E last_name: Avellaneda Sarrió orcid: 0000-0001-6406-524X - first_name: Nikola full_name: Canigova, Nikola id: 3795523E-F248-11E8-B48F-1D18A9856A87 last_name: Canigova orcid: 0000-0002-8518-5926 - first_name: Florian R full_name: Gärtner, Florian R id: 397A88EE-F248-11E8-B48F-1D18A9856A87 last_name: Gärtner orcid: 0000-0001-6120-3723 - first_name: Kari full_name: Vaahtomeri, Kari id: 368EE576-F248-11E8-B48F-1D18A9856A87 last_name: Vaahtomeri orcid: 0000-0001-7829-3518 - first_name: Michael full_name: Riedl, Michael id: 3BE60946-F248-11E8-B48F-1D18A9856A87 last_name: Riedl orcid: 0000-0003-4844-6311 - first_name: Ingrid full_name: De Vries, Ingrid id: 4C7D837E-F248-11E8-B48F-1D18A9856A87 last_name: De Vries - first_name: Jack full_name: Merrin, Jack id: 4515C308-F248-11E8-B48F-1D18A9856A87 last_name: Merrin orcid: 0000-0001-5145-4609 - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Yoshinori full_name: Fukui, Yoshinori last_name: Fukui - first_name: Alba full_name: Juanes Garcia, Alba id: 40F05888-F248-11E8-B48F-1D18A9856A87 last_name: Juanes Garcia orcid: 0000-0002-1009-9652 - first_name: Michael K full_name: Sixt, Michael K id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87 last_name: Sixt orcid: 0000-0002-6620-9179 citation: ama: Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 2025;26:1258–1266. doi:10.1038/s41590-025-02211-w apa: Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. Nature Immunology. Springer Nature. https://doi.org/10.1038/s41590-025-02211-w chicago: Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” Nature Immunology. Springer Nature, 2025. https://doi.org/10.1038/s41590-025-02211-w. ieee: P. Dos Reis Rodrigues et al., “Migrating immune cells globally coordinate protrusive forces,” Nature Immunology, vol. 26. Springer Nature, pp. 1258–1266, 2025. ista: Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266. mla: Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” Nature Immunology, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:10.1038/s41590-025-02211-w. short: P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266. corr_author: '1' date_created: 2025-07-27T22:01:26Z date_published: 2025-08-01T00:00:00Z date_updated: 2026-04-28T13:26:50Z day: '01' ddc: - '570' department: - _id: MiSi - _id: NanoFab - _id: Bio doi: 10.1038/s41590-025-02211-w external_id: isi: - '001529134300001' pmid: - '40664976' file: - access_level: open_access checksum: 0c725123dca7797c682609bff2c4c5ac content_type: application/pdf creator: dernst date_created: 2025-07-31T08:00:33Z date_updated: 2025-07-31T08:00:33Z file_id: '20096' file_name: 2025_NatureImmunology_ReisRodrigues.pdf file_size: 13514646 relation: main_file success: 1 file_date_updated: 2025-07-31T08:00:33Z fulldoi: https://doi.org/10.1038/s41590-025-02211-w has_accepted_license: '1' intvolume: ' 26' isi: 1 language: - iso: eng month: '08' oa: 1 oa_version: Published Version page: 1258–1266 pmid: 1 project: - _id: bd91e723-d553-11ed-ba76-fe7eeb2185fd grant_number: '101071793' name: 'Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces' - _id: c092d618-5a5b-11eb-8a69-f92e1e843fc8 grant_number: 944-2020 name: 'Bioelectric patrolling: the role of the local membrane potential in immune cell migration' publication: Nature Immunology publication_identifier: eissn: - 1529-2916 issn: - 1529-2908 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: link: - description: News on ISTA website relation: press_release url: https://ista.ac.at/en/news/bench-pressing-cells/ record: - id: '20149' relation: dissertation_contains status: public scopus_import: '1' status: public title: Migrating immune cells globally coordinate protrusive forces tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 26 year: '2025' ... --- OA_place: publisher OA_type: gold _id: '20146' abstract: - lang: eng text: "This criteria catalogue and the accompanying assessment questions were developed by a working group of KEMÖ (Kooperation E-Medien Österreich, the Austrian Academic Library Consortium). They are intended to support research institutions and organisations in the evaluation of Open Science Infrastructures. The 20 criteria outlined in the catalogue provide a structured basis for making informed decisions regarding the financial support of these infrastructures.\r\n\r\nThe assessment questions are intended to be completed by Open Science Infrastructures and can be shared with them accordingly." article_processing_charge: No author: - first_name: Paul full_name: Gredler, Paul last_name: Gredler - first_name: Christian full_name: Kaier, Christian last_name: Kaier - first_name: Patrick full_name: Danowski, Patrick id: 2EBD1598-F248-11E8-B48F-1D18A9856A87 last_name: Danowski orcid: 0000-0002-6026-4409 - first_name: Michael full_name: Zoyer, Michael last_name: Zoyer - first_name: Katharina full_name: Rieck, Katharina last_name: Rieck - first_name: Andreas full_name: Ferus, Andreas last_name: Ferus - first_name: Elisabeth full_name: Rosenberger, Elisabeth last_name: Rosenberger - first_name: Alexander full_name: Löffler, Alexander last_name: Löffler - first_name: Lisa full_name: Hofer, Lisa last_name: Hofer - first_name: Laura full_name: Still, Laura last_name: Still citation: ama: Gredler P, Kaier C, Danowski P, et al. Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures. Zenodo; 2025. doi:10.5281/zenodo.15269364 apa: Gredler, P., Kaier, C., Danowski, P., Zoyer, M., Rieck, K., Ferus, A., … Still, L. (2025). Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures. Zenodo. https://doi.org/10.5281/zenodo.15269364 chicago: Gredler, Paul, Christian Kaier, Patrick Danowski, Michael Zoyer, Katharina Rieck, Andreas Ferus, Elisabeth Rosenberger, Alexander Löffler, Lisa Hofer, and Laura Still. Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures. Zenodo, 2025. https://doi.org/10.5281/zenodo.15269364. ieee: P. Gredler et al., Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures. Zenodo, 2025. ista: Gredler P, Kaier C, Danowski P, Zoyer M, Rieck K, Ferus A, Rosenberger E, Löffler A, Hofer L, Still L. 2025. Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures, Zenodo,p. mla: Gredler, Paul, et al. Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures. Zenodo, 2025, doi:10.5281/zenodo.15269364. short: P. Gredler, C. Kaier, P. Danowski, M. Zoyer, K. Rieck, A. Ferus, E. Rosenberger, A. Löffler, L. Hofer, L. Still, Catalogue of Criteria for Assessing the Funding Eligibility of Open Science Infrastructures, Zenodo, 2025. date_created: 2025-08-07T11:10:14Z date_published: 2025-08-07T00:00:00Z date_updated: 2025-08-11T07:20:03Z day: '07' ddc: - '020' department: - _id: E-Lib doi: 10.5281/zenodo.15269364 fulldoi: https://doi.org/10.5281/zenodo.15269364 has_accepted_license: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.5281/zenodo.15269364 month: '08' oa: 1 oa_version: Published Version publication_status: published publisher: Zenodo status: public title: Catalogue of criteria for assessing the funding eligibility of Open Science infrastructures tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: working_paper user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2025' ... --- DOAJ_listed: '1' OA_place: publisher OA_type: gold PlanS_conform: '1' _id: '20295' abstract: - lang: eng text: 'Scanning Kelvin probe microscopy (SKPM) is a powerful technique for macroscopic imaging of the electrostatic potential above a surface. Though most often used to image work-function variations of conductive surfaces, it can also be used to probe the surface charge on insulating surfaces. In both cases, relating the measured potential to the underlying signal is non-trivial. Here, general relationships are derived between the measured SKPM voltage and the underlying source, revealing either can be cast as a convolution with an appropriately scaled point spread function (PSF). For charge that exists on a thin insulating layer above a conductor, the PSF has the same shape as what would occur from a work-function variation alone, differing by a simple scaling factor. This relationship is confirmed by: (1) backing it out from finite-element simulations of work-function and charge signals, and (2) experimentally comparing the measured PSF from a small work-function target to that from a small charge spot. This scaling factor is further validated by comparing SKPM charge measurements with Faraday cup measurements for highly charged samples from contact-charging experiments. These results highlight a heretofore unappreciated connection between SKPM voltage and charge signals, offering a rigorous recipe to extract either from experimental data.' acknowledged_ssus: - _id: M-Shop - _id: NanoFab - _id: ScienComp - _id: LifeSc acknowledgement: This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (Grant agreement No. 949120). This research was supported by the Scientific Service Units of The Institute of Science and Technology Austria (ISTA) through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing Facility, and Lab Support Facility. The authors wish to thank Dmytro Rak and Juan Carlos Sobarzo for letting us use their equipment. The authors wish to thank Evgeniia Volobueva for advice in preparing PFIB samples. The authors wish to thank the contributions of the whole Waitukaitis group for useful discussions and feedback. article_number: e00521 article_processing_charge: Yes article_type: original arxiv: 1 author: - first_name: Isaac C full_name: Lenton, Isaac C id: a550210f-223c-11ec-8182-e2d45e817efb last_name: Lenton orcid: 0000-0002-5010-6984 - first_name: Felix full_name: Pertl, Felix id: 6313aec0-15b2-11ec-abd3-ed67d16139af last_name: Pertl orcid: 0000-0003-0463-5794 - first_name: Lubuna B full_name: Shafeek, Lubuna B id: 3CD37A82-F248-11E8-B48F-1D18A9856A87 last_name: Shafeek orcid: 0000-0001-7180-6050 - first_name: Scott R full_name: Waitukaitis, Scott R id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87 last_name: Waitukaitis orcid: 0000-0002-2299-3176 citation: ama: Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. A duality between surface charge and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces. 2025;12(19). doi:10.1002/admi.202500521 apa: Lenton, I. C., Pertl, F., Shafeek, L. B., & Waitukaitis, S. R. (2025). A duality between surface charge and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces. Wiley. https://doi.org/10.1002/admi.202500521 chicago: Lenton, Isaac C, Felix Pertl, Lubuna B Shafeek, and Scott R Waitukaitis. “A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.” Advanced Materials Interfaces. Wiley, 2025. https://doi.org/10.1002/admi.202500521. ieee: I. C. Lenton, F. Pertl, L. B. Shafeek, and S. R. Waitukaitis, “A duality between surface charge and work function in scanning Kelvin probe microscopy,” Advanced Materials Interfaces, vol. 12, no. 19. Wiley, 2025. ista: Lenton IC, Pertl F, Shafeek LB, Waitukaitis SR. 2025. A duality between surface charge and work function in scanning Kelvin probe microscopy. Advanced Materials Interfaces. 12(19), e00521. mla: Lenton, Isaac C., et al. “A Duality between Surface Charge and Work Function in Scanning Kelvin Probe Microscopy.” Advanced Materials Interfaces, vol. 12, no. 19, e00521, Wiley, 2025, doi:10.1002/admi.202500521. short: I.C. Lenton, F. Pertl, L.B. Shafeek, S.R. Waitukaitis, Advanced Materials Interfaces 12 (2025). corr_author: '1' date_created: 2025-09-07T22:01:33Z date_published: 2025-10-01T00:00:00Z date_updated: 2025-12-30T09:31:25Z day: '01' ddc: - '530' department: - _id: ScWa - _id: NanoFab doi: 10.1002/admi.202500521 ec_funded: 1 external_id: arxiv: - '2506.07187' isi: - '001560163400001' file: - access_level: open_access checksum: 906fcc7733be8ce8a83600427b82cd5a content_type: application/pdf creator: dernst date_created: 2025-12-30T09:31:11Z date_updated: 2025-12-30T09:31:11Z file_id: '20908' file_name: 2025_AdvMaterialsInterfaces_Lenton.pdf file_size: 1830117 relation: main_file success: 1 file_date_updated: 2025-12-30T09:31:11Z fulldoi: https://doi.org/10.1002/admi.202500521 has_accepted_license: '1' intvolume: ' 12' isi: 1 issue: '19' language: - iso: eng month: '10' oa: 1 oa_version: Published Version project: - _id: 0aa60e99-070f-11eb-9043-a6de6bdc3afa call_identifier: H2020 grant_number: '949120' name: 'Tribocharge: a multi-scale approach to an enduring problem in physics' publication: Advanced Materials Interfaces publication_identifier: eissn: - 2196-7350 publication_status: published publisher: Wiley quality_controlled: '1' scopus_import: '1' status: public title: A duality between surface charge and work function in scanning Kelvin probe microscopy tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 12 year: '2025' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '20321' abstract: - lang: eng text: Microsecond-to-millisecond motions are instrumental for many biomolecular functions, including enzymatic activity and ligand binding. Bloch-McConnell Relaxation Dispersion (BMRD) Nuclear Magnetic Resonance (NMR) spectroscopy is a key technique for studying these dynamic processes. While BMRD experiments are routinely used to probe protein motions in solution, the experiment is more demanding in the solid state, where dipolar couplings complicate the spin dynamics. It is believed that high deuteration levels are required and sufficient to obtain accurate and quantitative data. Here we show that even under fast magic-angle spinning and high levels of deuteration artifactual “bumps” in 15N R1ρ BMRD profiles are common. The origin of these artifacts is identified as a second-order three-spin Mixed Rotational and Rotary Resonance (MIRROR) recoupling condition. These artifacts are found to be a significant confounding factor for the accurate quantification of microsecond protein dynamics using BMRD in the solid state. We show that the application of low-power continuous wave (CW) decoupling simultaneously with the 15N spin-lock leads to the suppression of these conditions and enables quantitative measurements of microsecond exchange in the solid state. Remarkably, the application of decoupling allows the measurement of accurate BMRD even in fully protonated proteins at 100 kHz MAS, thus extending the scope of μs dynamics measurements in MAS NMR. acknowledged_ssus: - _id: NMR - _id: LifeSc acknowledgement: The authors thank Alexey Krushelnitsky for useful discussions. C.P.J. thanks NSF (MCB-2303862) and NIH (R35GM156238 and S10OD012303) for funding. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria (ISTA) through resources provided by the Nuclear Magnetic Resonance and the Lab Support Facilities. article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Benjamin full_name: Tatman, Benjamin id: 71cda2f3-e604-11ee-a1df-da10587eda3f last_name: Tatman - first_name: Vidhyalakshmi full_name: Sridharan, Vidhyalakshmi last_name: Sridharan - first_name: Motilal full_name: Uttarkabat, Motilal last_name: Uttarkabat - first_name: Christopher P. full_name: Jaroniec, Christopher P. last_name: Jaroniec - first_name: Matthias full_name: Ernst, Matthias last_name: Ernst - first_name: Petra full_name: Rovo, Petra id: c316e53f-b965-11eb-b128-bb26acc59c00 last_name: Rovo orcid: 0000-0001-8729-7326 - first_name: Paul full_name: Schanda, Paul id: 7B541462-FAF6-11E9-A490-E8DFE5697425 last_name: Schanda orcid: 0000-0002-9350-7606 citation: ama: 'Tatman B, Sridharan V, Uttarkabat M, et al. Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR. Journal of the American Chemical Society. 2025;147(32):29315-29326. doi:10.1021/jacs.5c09057' apa: 'Tatman, B., Sridharan, V., Uttarkabat, M., Jaroniec, C. P., Ernst, M., Rovo, P., & Schanda, P. (2025). Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR. Journal of the American Chemical Society. American Chemical Society. https://doi.org/10.1021/jacs.5c09057' chicago: 'Tatman, Benjamin, Vidhyalakshmi Sridharan, Motilal Uttarkabat, Christopher P. Jaroniec, Matthias Ernst, Petra Rovo, and Paul Schanda. “Bumps on the Road: The Way to Clean Relaxation Dispersion Magic-Angle Spinning NMR.” Journal of the American Chemical Society. American Chemical Society, 2025. https://doi.org/10.1021/jacs.5c09057.' ieee: 'B. Tatman et al., “Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR,” Journal of the American Chemical Society, vol. 147, no. 32. American Chemical Society, pp. 29315–29326, 2025.' ista: 'Tatman B, Sridharan V, Uttarkabat M, Jaroniec CP, Ernst M, Rovo P, Schanda P. 2025. Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR. Journal of the American Chemical Society. 147(32), 29315–29326.' mla: 'Tatman, Benjamin, et al. “Bumps on the Road: The Way to Clean Relaxation Dispersion Magic-Angle Spinning NMR.” Journal of the American Chemical Society, vol. 147, no. 32, American Chemical Society, 2025, pp. 29315–26, doi:10.1021/jacs.5c09057.' short: B. Tatman, V. Sridharan, M. Uttarkabat, C.P. Jaroniec, M. Ernst, P. Rovo, P. Schanda, Journal of the American Chemical Society 147 (2025) 29315–29326. corr_author: '1' date_created: 2025-09-10T05:37:19Z date_published: 2025-08-01T00:00:00Z date_updated: 2026-06-10T08:33:41Z day: '01' ddc: - '540' department: - _id: PaSc - _id: NMR doi: 10.1021/jacs.5c09057 external_id: isi: - '001542746200001' pmid: - '40748291' file: - access_level: open_access checksum: b350d56ddddefea96cebd62c277c0ff5 content_type: application/pdf creator: dernst date_created: 2025-09-10T07:53:10Z date_updated: 2025-09-10T07:53:10Z file_id: '20337' file_name: 2025_JACS_Tatman.pdf file_size: 5235353 relation: main_file success: 1 file_date_updated: 2025-09-10T07:53:10Z fulldoi: https://doi.org/10.1021/jacs.5c09057 has_accepted_license: '1' intvolume: ' 147' isi: 1 issue: '32' language: - iso: eng month: '08' oa: 1 oa_version: Published Version page: 29315-29326 pmid: 1 publication: Journal of the American Chemical Society publication_identifier: eissn: - 1520-5126 issn: - 0002-7863 publication_status: published publisher: American Chemical Society quality_controlled: '1' related_material: record: - id: '19696' relation: used_in_publication status: public scopus_import: '1' status: public title: 'Bumps on the road: The way to clean relaxation dispersion magic-angle spinning NMR' tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 147 year: '2025' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '20326' abstract: - lang: eng text: Ag2Se is a promising n-type thermoelectric material, but its performance is limited by excessive carrier concentration, compositional inhomogeneity, and phase instability, challenges rooted in a narrow homogeneity range and uncontrolled Ag+ diffusion in the superionic phase. Here, we address these issues by exploiting liquid–solid interface reactions using CdSe complexes that remove surface excess Ag to yield stoichiometric Ag2Se and generate CdSe nanodomains that inhibit Ag+ diffusion and constrain grain growth. The resulting Ag2Se-CdSe nanocomposites exhibit a reproducible, stable figure of merit (zT) of 1.04 between 300 and 390 K. Beyond demonstrating high performance, we elucidate the interfacial chemical reactions that give rise to the observed microstructure and transport properties, providing a foundation for rationally engineering interfacial chemistry to tailor transport properties across diverse thermoelectric material systems. acknowledged_ssus: - _id: EM-Fac - _id: LifeSc - _id: NanoFab - _id: MassSpec acknowledgement: 'M.I. acknowledges financial support from ISTA and the Werner Siemens Foundation. The Scientific Service Units (SSU) of ISTA supported this work through resources provided by the Electron Microscopy Facility (EMF), the Lab Support Facility (LSF) and the Nanofabrication Facility (NNF) and the LSF Mass Spectrometry Service. The members of the Ibáñez research group are acknowledged, especially Christine Fiedler for scientific illustration and Ihor Cherniukh for valuable discussions. Y.L. acknowledges funding from the National Natural Science Foundation of China (NSFC) (Grants No. 22209034), the Innovation and Entrepreneurship Project of Overseas Returnees in Anhui Province (Grant No. 2022LCX002) and the Fundamental Research Funds for the Central Universities (JZ2024HGTB0239). K.H.L. acknowledges financial support from the National Natural Science Foundation of China (NSFC) (Grant No. 22208293). ICN2 acknowledges funding from Generalitat de Catalunya 2021SGR00457. Authors acknowledge the Advanced Materials programme by the Spanish Government with funding from European Union NextGenerationEU (PRTR-C17.I1) and by Generalitat de Catalunya (Project In-CAEM). The authors thank support from the project AMaDE (PID2023-149158OB-C43), funded by MCIN/AEI/10.13039/501100011033/and by “ERDF Away of making Europe”, by the “European Union”. ICN2 is supported by the Severo Ochoa program from Spanish MCIN/AEI (Grant No.: CEX2021-001214-S) and is funded by the CERCA Programme/Generalitat de Catalunya. ICN2 is founding member of e-DREAM. (68) M.H. acknowledges the funding from the Australian Research Council (FT230100316 and IH200100035). M.H. acknowledges the computational support from the National Computational Infrastructure (NCI) and Pawsey Supercomputing Centre, Australia.' article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Yu full_name: Liu, Yu id: 2A70014E-F248-11E8-B48F-1D18A9856A87 last_name: Liu orcid: 0000-0001-7313-6740 - first_name: Tobias full_name: Kleinhanns, Tobias id: 8BD9DE16-AB3C-11E9-9C8C-2A03E6697425 last_name: Kleinhanns orcid: 0000-0003-1537-7436 - first_name: Sharona full_name: Horta, Sharona id: 03a7e858-01b1-11ec-8b71-99ae6c4a05bc last_name: Horta - first_name: Ewelina full_name: Dutkiewicz, Ewelina id: 0601cc46-c082-11ec-9b07-bb29641d1de9 last_name: Dutkiewicz - first_name: Shaoqing full_name: Lu, Shaoqing last_name: Lu - first_name: Maria Chiara full_name: Spadaro, Maria Chiara last_name: Spadaro - first_name: Aziz full_name: Genç, Aziz last_name: Genç - first_name: Lei full_name: Chen, Lei last_name: Chen - first_name: Khak Ho full_name: Lim, Khak Ho last_name: Lim - first_name: Min full_name: Hong, Min last_name: Hong - first_name: Jordi full_name: Arbiol, Jordi last_name: Arbiol - first_name: Maria full_name: Ibáñez, Maria id: 43C61214-F248-11E8-B48F-1D18A9856A87 last_name: Ibáñez orcid: 0000-0001-5013-2843 citation: ama: Liu Y, Kleinhanns T, Horta S, et al. Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se. Journal of the American Chemical Society. 2025;147(35):32199-32208. doi:10.1021/jacs.5c11435 apa: Liu, Y., Kleinhanns, T., Horta, S., Dutkiewicz, E., Lu, S., Spadaro, M. C., … Ibáñez, M. (2025). Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se. Journal of the American Chemical Society. American Chemical Society. https://doi.org/10.1021/jacs.5c11435 chicago: Liu, Yu, Tobias Kleinhanns, Sharona Horta, Ewelina Dutkiewicz, Shaoqing Lu, Maria Chiara Spadaro, Aziz Genç, et al. “Liquid-Solid Interface Reactions Drive Enhanced Thermoelectric Performance in Ag2Se.” Journal of the American Chemical Society. American Chemical Society, 2025. https://doi.org/10.1021/jacs.5c11435. ieee: Y. Liu et al., “Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se,” Journal of the American Chemical Society, vol. 147, no. 35. American Chemical Society, pp. 32199–32208, 2025. ista: Liu Y, Kleinhanns T, Horta S, Dutkiewicz E, Lu S, Spadaro MC, Genç A, Chen L, Lim KH, Hong M, Arbiol J, Ibáñez M. 2025. Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se. Journal of the American Chemical Society. 147(35), 32199–32208. mla: Liu, Yu, et al. “Liquid-Solid Interface Reactions Drive Enhanced Thermoelectric Performance in Ag2Se.” Journal of the American Chemical Society, vol. 147, no. 35, American Chemical Society, 2025, pp. 32199–208, doi:10.1021/jacs.5c11435. short: Y. Liu, T. Kleinhanns, S. Horta, E. Dutkiewicz, S. Lu, M.C. Spadaro, A. Genç, L. Chen, K.H. Lim, M. Hong, J. Arbiol, M. Ibáñez, Journal of the American Chemical Society 147 (2025) 32199–32208. corr_author: '1' date_created: 2025-09-10T05:44:03Z date_published: 2025-08-22T00:00:00Z date_updated: 2026-07-28T09:55:14Z day: '22' ddc: - '540' department: - _id: MaIb - _id: MassSpec doi: 10.1021/jacs.5c11435 external_id: isi: - '001558320100001' file: - access_level: open_access checksum: 52892fa91adadd39a1c42da9e01139a5 content_type: application/pdf creator: dernst date_created: 2025-09-10T06:55:17Z date_updated: 2025-09-10T06:55:17Z file_id: '20334' file_name: 2025_JACS_Liu.pdf file_size: 9997327 relation: main_file success: 1 file_date_updated: 2025-09-10T06:55:17Z fulldoi: https://doi.org/10.1021/jacs.5c11435 has_accepted_license: '1' intvolume: ' 147' isi: 1 issue: '35' language: - iso: eng month: '08' oa: 1 oa_version: Published Version page: 32199-32208 project: - _id: 9B8F7476-BA93-11EA-9121-9846C619BF3A name: 'HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery' publication: Journal of the American Chemical Society publication_identifier: eissn: - 1520-5126 issn: - 0002-7863 publication_status: published publisher: American Chemical Society quality_controlled: '1' related_material: record: - id: '22017' relation: dissertation_contains status: public scopus_import: '1' status: public title: Liquid-solid interface reactions drive enhanced thermoelectric performance in Ag2Se tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 147 year: '2025' ... --- OA_place: publisher OA_type: hybrid _id: '19278' abstract: - lang: eng text: 'When two insulating, neutral materials are contacted and separated, they exchange electrical charge1. Experiments have long suggested that this ‘contact electrification’ is transitive, with different materials ordering into ‘triboelectric series’ based on the sign of charge acquired2. At the same time, the effect is plagued by unpredictability, preventing consensus on the mechanism and casting doubt on the rhyme and reason that series imply3. Here we expose an unanticipated connection between the unpredictability and order in contact electrification: nominally identical materials initially exchange charge randomly and intransitively, but—over repeated experiments—order into triboelectric series. We find that this evolution is driven by the act of contact itself—samples with more contacts in their history charge negatively to ones with fewer contacts. Capturing this ‘contact bias’ in a minimal model, we recreate both the initial randomness and ultimate order in numerical simulations and use it experimentally to force the appearance of a triboelectric series of our choosing. With a set of surface-sensitive techniques to search for the underlying alterations contact creates, we only find evidence of nanoscale morphological changes, pointing to a mechanism strongly coupled with mechanics. Our results highlight the centrality of contact history in contact electrification and suggest that focusing on the unpredictability that has long plagued the effect may hold the key to understanding it.' acknowledged_ssus: - _id: M-Shop - _id: NanoFab - _id: ScienComp - _id: EM-Fac - _id: LifeSc acknowledgement: This project has received financing from the European Research Council grant agreement no. 949120 under the European Union’s Horizon 2020 research and innovation programme. The Analytical Instrumentation Center of the TU Wien acknowledges support by the FFG project ‘ELSA’ under grant no. 884672. C.M.P. and M.O. acknowledge the state of Lower Austria and the European Regional Development Fund under grant no. WST3-F-542638/004-2021. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Miba Machine Shop, Nanofabrication Facility, Scientific Computing facility, Electron Microscopy Facility and Lab Support Facility. We thank J. Garcia-Suarez and G. Anciaux for the suggestion to look into the roughness power spectral density. We thank I.-M. Strugaru for help with testing the device for Young’s modulus measurements. Open access funding provided by Institute of Science and Technology (IST Austria). article_number: 664-669 article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Juan Carlos A full_name: Sobarzo Ponce, Juan Carlos A id: 4B807D68-AE37-11E9-AC72-31CAE5697425 last_name: Sobarzo Ponce - first_name: Felix full_name: Pertl, Felix id: 6313aec0-15b2-11ec-abd3-ed67d16139af last_name: Pertl orcid: 0000-0003-0463-5794 - first_name: Daniel full_name: Balazs, Daniel id: 302BADF6-85FC-11EA-9E3B-B9493DDC885E last_name: Balazs orcid: 0000-0001-7597-043X - first_name: Tommaso full_name: Costanzo, Tommaso id: D93824F4-D9BA-11E9-BB12-F207E6697425 last_name: Costanzo orcid: 0000-0001-9732-3815 - first_name: Markus full_name: Sauer, Markus last_name: Sauer - first_name: Annette full_name: Foelske, Annette last_name: Foelske - first_name: Markus full_name: Ostermann, Markus last_name: Ostermann - first_name: Christian M. full_name: Pichler, Christian M. last_name: Pichler - first_name: Yongkang full_name: Wang, Yongkang last_name: Wang - first_name: Yuki full_name: Nagata, Yuki last_name: Nagata - first_name: Mischa full_name: Bonn, Mischa last_name: Bonn - first_name: Scott R full_name: Waitukaitis, Scott R id: 3A1FFC16-F248-11E8-B48F-1D18A9856A87 last_name: Waitukaitis orcid: 0000-0002-2299-3176 citation: ama: Sobarzo Ponce JCA, Pertl F, Balazs D, et al. Spontaneous ordering of identical materials into a triboelectric series. Nature. 2025;638(8051). doi:10.1038/s41586-024-08530-6 apa: Sobarzo Ponce, J. C. A., Pertl, F., Balazs, D., Costanzo, T., Sauer, M., Foelske, A., … Waitukaitis, S. R. (2025). Spontaneous ordering of identical materials into a triboelectric series. Nature. Springer Nature. https://doi.org/10.1038/s41586-024-08530-6 chicago: Sobarzo Ponce, Juan Carlos A, Felix Pertl, Daniel Balazs, Tommaso Costanzo, Markus Sauer, Annette Foelske, Markus Ostermann, et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” Nature. Springer Nature, 2025. https://doi.org/10.1038/s41586-024-08530-6. ieee: J. C. A. Sobarzo Ponce et al., “Spontaneous ordering of identical materials into a triboelectric series,” Nature, vol. 638, no. 8051. Springer Nature, 2025. ista: Sobarzo Ponce JCA, Pertl F, Balazs D, Costanzo T, Sauer M, Foelske A, Ostermann M, Pichler CM, Wang Y, Nagata Y, Bonn M, Waitukaitis SR. 2025. Spontaneous ordering of identical materials into a triboelectric series. Nature. 638(8051), 664–669. mla: Sobarzo Ponce, Juan Carlos A., et al. “Spontaneous Ordering of Identical Materials into a Triboelectric Series.” Nature, vol. 638, no. 8051, 664–669, Springer Nature, 2025, doi:10.1038/s41586-024-08530-6. short: J.C.A. Sobarzo Ponce, F. Pertl, D. Balazs, T. Costanzo, M. Sauer, A. Foelske, M. Ostermann, C.M. Pichler, Y. Wang, Y. Nagata, M. Bonn, S.R. Waitukaitis, Nature 638 (2025). corr_author: '1' date_created: 2025-03-02T23:01:52Z date_published: 2025-02-20T00:00:00Z date_updated: 2026-08-27T11:42:43Z day: '20' ddc: - '530' department: - _id: ScWa - _id: LifeSc - _id: EM-Fac doi: 10.1038/s41586-024-08530-6 ec_funded: 1 external_id: isi: - '001428076100015' pmid: - '39972227' file: - access_level: open_access checksum: fecf302274dd3218d3e7dd22f39a6c0c content_type: application/pdf creator: dernst date_created: 2025-03-04T10:05:18Z date_updated: 2025-03-04T10:05:18Z file_id: '19289' file_name: 2025_Nature_Sobarzo.pdf file_size: 3807415 relation: main_file success: 1 file_date_updated: 2025-03-04T10:05:18Z fulldoi: https://doi.org/10.1038/s41586-024-08530-6 has_accepted_license: '1' intvolume: ' 638' isi: 1 issue: '8051' language: - iso: eng month: '02' oa: 1 oa_version: Published Version pmid: 1 project: - _id: 0aa60e99-070f-11eb-9043-a6de6bdc3afa call_identifier: H2020 grant_number: '949120' name: 'Tribocharge: a multi-scale approach to an enduring problem in physics' publication: Nature publication_identifier: eissn: - 1476-4687 issn: - 0028-0836 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: link: - description: News on ISTA website relation: press_release url: https://ista.ac.at/en/news/an-electrifying-turn-in-an-age-old-quest/ record: - id: '20203' relation: dissertation_contains status: public - id: '22684' relation: dissertation_contains status: public scopus_import: '1' status: public title: Spontaneous ordering of identical materials into a triboelectric series tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 638 year: '2025' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '17468' abstract: - lang: eng text: Oxygen redox chemistry is central to life1 and many human-made technologies, such as in energy storage2,3,4. The large energy gain from oxygen redox reactions is often connected with the occurrence of harmful reactive oxygen species3,5,6. Key species are superoxide and the highly reactive singlet oxygen3,4,5,6,7, which may evolve from superoxide. However, the factors determining the formation of singlet oxygen, rather than the relatively unreactive triplet oxygen, are unknown. Here we report that the release of triplet or singlet oxygen is governed by individual Marcus normal and inverted region behaviour. We found that as the driving force for the reaction increases, the initially dominant evolution of triplet oxygen slows down, and singlet oxygen evolution becomes predominant with higher maximum kinetics. This behaviour also applies to the widely observed superoxide disproportionation, in which one superoxide is oxidized by another, in both non-aqueous and aqueous systems, with Lewis and Brønsted acidity controlling the driving forces. Singlet oxygen yields governed by these conditions are relevant, for example, in batteries or cellular organelles in which superoxide forms. Our findings suggest ways to understand and control spin states and kinetics in oxygen redox chemistry, with implications for fields, including life sciences, pure chemistry and energy storage. acknowledged_ssus: - _id: Bio - _id: LifeSc - _id: M-Shop - _id: ScienComp acknowledgement: S.A.F. thanks the Institute of Science and Technology Austria (ISTA) for the support. The Scientific Service Units of ISTA supported this research through resources provided by the Imaging and Optics Facility, the Lab Support Facility, the Miba Machine Shop and Scientific Computing. This research was partly funded by the Austrian Science Fund (FWF) (10.55776/P37169 and 10.55776/COE5). For open access purposes, the author has applied for a CC BY public copyright licence to any author-accepted manuscript version arising from this submission. R.H. acknowledges funding through CZI grant DAF2020-225401 (10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (10.13039/100014989). H.T.K.N. acknowledges funding by the European Commission Erasmus Mundus Joint Masters programme. We thank M. Sixt and M. Chinon for the discussions about O-redox in life and R. Jethwa for proofreading. Open access funding was provided by ISTA. article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Soumyadip full_name: Mondal, Soumyadip id: d25d21ef-dc8d-11ea-abe3-ec4576307f48 last_name: Mondal - first_name: Huyen T.K. full_name: Nguyen, Huyen T.K. last_name: Nguyen - first_name: Robert full_name: Hauschild, Robert id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87 last_name: Hauschild orcid: 0000-0001-9843-3522 - first_name: Stefan Alexander full_name: Freunberger, Stefan Alexander id: A8CA28E6-CE23-11E9-AD2D-EC27E6697425 last_name: Freunberger orcid: 0000-0003-2902-5319 citation: ama: Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. Nature. 2025;646(8085):601–605. doi:10.1038/s41586-025-09587-7 apa: Mondal, S., Nguyen, H. T. K., Hauschild, R., & Freunberger, S. A. (2025). Marcus kinetics control singlet and triplet oxygen evolving from superoxide. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-09587-7 chicago: Mondal, Soumyadip, Huyen T.K. Nguyen, Robert Hauschild, and Stefan Alexander Freunberger. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” Nature. Springer Nature, 2025. https://doi.org/10.1038/s41586-025-09587-7. ieee: S. Mondal, H. T. K. Nguyen, R. Hauschild, and S. A. Freunberger, “Marcus kinetics control singlet and triplet oxygen evolving from superoxide,” Nature, vol. 646, no. 8085. Springer Nature, pp. 601–605, 2025. ista: Mondal S, Nguyen HTK, Hauschild R, Freunberger SA. 2025. Marcus kinetics control singlet and triplet oxygen evolving from superoxide. Nature. 646(8085), 601–605. mla: Mondal, Soumyadip, et al. “Marcus Kinetics Control Singlet and Triplet Oxygen Evolving from Superoxide.” Nature, vol. 646, no. 8085, Springer Nature, 2025, pp. 601–605, doi:10.1038/s41586-025-09587-7. short: S. Mondal, H.T.K. Nguyen, R. Hauschild, S.A. Freunberger, Nature 646 (2025) 601–605. corr_author: '1' date_created: 2024-08-29T10:40:23Z date_published: 2025-10-16T00:00:00Z date_updated: 2026-09-16T06:53:54Z day: '16' ddc: - '540' department: - _id: StFr - _id: Bio doi: 10.1038/s41586-025-09587-7 external_id: isi: - '001586378900001' pmid: - '41044415' file: - access_level: open_access checksum: b507ddd23df0388aa65d04dc9b00fe3d content_type: application/pdf creator: dernst date_created: 2025-10-20T10:26:13Z date_updated: 2025-10-20T10:26:13Z file_id: '20500' file_name: 2025_Nature_Mondal.pdf file_size: 3809247 relation: main_file success: 1 file_date_updated: 2025-10-20T10:26:13Z fulldoi: https://doi.org/10.1038/s41586-025-09587-7 has_accepted_license: '1' intvolume: ' 646' isi: 1 issue: '8085' language: - iso: eng month: '10' oa: 1 oa_version: Published Version page: 601–605 pmid: 1 project: - _id: 8df062be-16d5-11f0-9cad-f559b6612c7e grant_number: P37169 name: Singlet oxygen in non-aqueous oxygen redox chemistry - _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473 grant_number: CZI01 name: Tools for automation and feedback microscopy - _id: 5eaf4378-b033-11f1-b276-f928018a46c1 grant_number: COE05 name: Materials for Energy Conversion and Storage (Freunberger) publication: Nature publication_identifier: eissn: - 1476-4687 issn: - 0028-0836 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: link: - description: News on ISTA website relation: press_release url: https://ista.ac.at/en/news/taming-the-bad-oxygen/ scopus_import: '1' status: public title: Marcus kinetics control singlet and triplet oxygen evolving from superoxide tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 646 year: '2025' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '18449' abstract: - lang: eng text: "Research involving human subjects or identifiable human material and data must be assessed by an ethics committee. The Karl Landsteiner University of Health Sciences has established a Commission on Ethics and Scientific Integrity to evaluate medical research conducted by its faculty and students and at its affiliated hospitals.\r\nAll projects submitted to the Commission on Ethics and Scientific Integrity between 2018 and 2023 were analyzed regarding their major characteristics, the duration of the evaluation process, and votes issued.\r\nA total of 520 applications were electronically submitted during the observation period. Most of the studies were retrospective data analyses in the field of oncology, psychology and surgery. Most studies included less than 100 volunteers. Of the applications 50% received a final vote within 5 months, during which several revision rounds took place. Overall, about 77% of votes issued during the observation period were positive and 2% were rejections. In 11% files were closed due to withdrawal. In 11% final votes were pending at the end of the observation period due to requests for revisions.\r\nOur results emphasize the importance of institutional ethics committees using the example of the Commission on Ethics and Scientific Integrity at the Karl Landsteiner University. Such committees fill a gap in evaluating research not covered by Austrian legal regulations. Continuous development of standards, operating procedures, and national and international collaborations are required to assess and minimize risks to trial subjects and to provide a safe and productive environment for research in human medicine and related fields." acknowledgement: Open access funding provided by Karl Landsteiner University. article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Sophie full_name: Schober, Sophie id: 80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8 last_name: Schober - first_name: Sascha full_name: Klee, Sascha last_name: Klee - first_name: Franz full_name: Trautinger, Franz last_name: Trautinger citation: ama: 'Schober S, Klee S, Trautinger F. The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023. Wiener Klinische Wochenschrift. 2025;137:432-437. doi:10.1007/s00508-024-02462-x' apa: 'Schober, S., Klee, S., & Trautinger, F. (2025). The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023. Wiener Klinische Wochenschrift. Springer Nature. https://doi.org/10.1007/s00508-024-02462-x' chicago: 'Schober, Sophie, Sascha Klee, and Franz Trautinger. “The Role of Institutional Ethics Committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023.” Wiener Klinische Wochenschrift. Springer Nature, 2025. https://doi.org/10.1007/s00508-024-02462-x.' ieee: 'S. Schober, S. Klee, and F. Trautinger, “The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023,” Wiener Klinische Wochenschrift, vol. 137. Springer Nature, pp. 432–437, 2025.' ista: 'Schober S, Klee S, Trautinger F. 2025. The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023. Wiener Klinische Wochenschrift. 137, 432–437.' mla: 'Schober, Sophie, et al. “The Role of Institutional Ethics Committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023.” Wiener Klinische Wochenschrift, vol. 137, Springer Nature, 2025, pp. 432–37, doi:10.1007/s00508-024-02462-x.' short: S. Schober, S. Klee, F. Trautinger, Wiener Klinische Wochenschrift 137 (2025) 432–437. corr_author: '1' das_tickbox: '0' date_created: 2024-10-20T22:02:07Z date_published: 2025-07-01T00:00:00Z date_updated: 2026-09-17T10:27:37Z day: '01' ddc: - '570' department: - _id: PreCl doi: 10.1007/s00508-024-02462-x external_id: isi: - '001329812000001' file: - access_level: open_access checksum: 321be8a584117feaea9f3feaa28caabd content_type: application/pdf creator: dernst date_created: 2025-12-30T06:54:03Z date_updated: 2025-12-30T06:54:03Z file_id: '20880' file_name: 2025_WrKlinischeWochenschrift_Schober.pdf file_size: 580791 relation: main_file success: 1 file_date_updated: 2025-12-30T06:54:03Z fulldoi: https://doi.org/10.1007/s00508-024-02462-x has_accepted_license: '1' intvolume: ' 137' isi: 1 language: - iso: eng month: '07' oa: 1 oa_version: Published Version page: 432-437 publication: Wiener Klinische Wochenschrift publication_identifier: eissn: - 1613-7671 issn: - 0043-5325 publication_status: published publisher: Springer Nature quality_controlled: '1' researchdata_availability: no scopus_import: '1' status: public supplementarymaterial: no title: 'The role of institutional ethics committees in Austria: Report of the Commission on Ethics and Scientific Integrity of the Karl Landsteiner University of Health Sciences 2018–2023' tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345 volume: 137 year: '2025' ... --- OA_place: publisher OA_type: hybrid PlanS_conform: '1' _id: '19704' abstract: - lang: eng text: The information-processing capability of the brain’s cellular network depends on the physical wiring pattern between neurons and their molecular and functional characteristics. Mapping neurons and resolving their individual synaptic connections can be achieved by volumetric imaging at nanoscale resolution1,2 with dense cellular labelling. Light microscopy is uniquely positioned to visualize specific molecules, but dense, synapse-level circuit reconstruction by light microscopy has been out of reach, owing to limitations in resolution, contrast and volumetric imaging capability. Here we describe light-microscopy-based connectomics (LICONN). We integrated specifically engineered hydrogel embedding and expansion with comprehensive deep-learning-based segmentation and analysis of connectivity, thereby directly incorporating molecular information into synapse-level reconstructions of brain tissue. LICONN will allow synapse-level phenotyping of brain tissue in biological experiments in a readily adoptable manner. acknowledged_ssus: - _id: Bio - _id: LifeSc - _id: ScienComp - _id: PreCl - _id: M-Shop - _id: E-Lib acknowledgement: 'We thank S. Dorkenwald and P. Li for critical reading of the manuscript, S. Loomba for discussions and E. Miguel for support with data handling. We acknowledge support from ISTA’s scientific service units: Imaging and Optics, Lab Support, Scientific Computing, the preclinical facility, the Miba Machine Shop and the library. We acknowledge funding from the following sources: Austrian Science Fund (FWF) grant DK W1232 (J.G.D. and M.R.T.); Austrian Academy of Sciences DOC fellowship 26137 (M.R.T.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); the European Union’s Horizon 2020 research and innovation programme and Marie Skłodowska-Curie Actions Fellowship 665385 (J.L.); and the European Union’s Horizon 2020 research and innovation programme and European Research Council (ERC) grant 101044865 ‘SecretAutism’ (G.N.).Open access funding provided by Institute of Science and Technology (IST Austria).' article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Mojtaba full_name: Tavakoli, Mojtaba id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87 last_name: Tavakoli orcid: 0000-0002-7667-6854 - first_name: Julia full_name: Lyudchik, Julia id: 46E28B80-F248-11E8-B48F-1D18A9856A87 last_name: Lyudchik - first_name: Michał full_name: Januszewski, Michał last_name: Januszewski - first_name: Vitali full_name: Vistunou, Vitali id: 7e146587-8972-11ed-ae7b-d7a32ea86a81 last_name: Vistunou - first_name: Nathalie full_name: Agudelo Duenas, Nathalie id: 40E7F008-F248-11E8-B48F-1D18A9856A87 last_name: Agudelo Duenas - first_name: Jakob full_name: Vorlaufer, Jakob id: 937696FA-C996-11E9-8C7C-CF13E6697425 last_name: Vorlaufer orcid: 0009-0000-7590-3501 - first_name: Christoph M full_name: Sommer, Christoph M id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87 last_name: Sommer orcid: 0000-0003-1216-9105 - first_name: Caroline full_name: Kreuzinger, Caroline id: 382077BA-F248-11E8-B48F-1D18A9856A87 last_name: Kreuzinger - first_name: Bárbara full_name: Oliveira, Bárbara id: 3B03AA1A-F248-11E8-B48F-1D18A9856A87 last_name: Oliveira - first_name: Alban full_name: Cenameri, Alban id: 9ac8f577-2357-11eb-997a-e566c5550886 last_name: Cenameri - first_name: Gaia full_name: Novarino, Gaia id: 3E57A680-F248-11E8-B48F-1D18A9856A87 last_name: Novarino orcid: 0000-0002-7673-7178 - first_name: Viren full_name: Jain, Viren last_name: Jain - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 citation: ama: Tavakoli M, Lyudchik J, Januszewski M, et al. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. Nature. 2025;642:398-410. doi:10.1038/s41586-025-08985-1 apa: Tavakoli, M., Lyudchik, J., Januszewski, M., Vistunou, V., Agudelo Duenas, N., Vorlaufer, J., … Danzl, J. G. (2025). Light-microscopy-based connectomic reconstruction of mammalian brain tissue. Nature. Springer Nature. https://doi.org/10.1038/s41586-025-08985-1 chicago: Tavakoli, Mojtaba, Julia Lyudchik, Michał Januszewski, Vitali Vistunou, Nathalie Agudelo Duenas, Jakob Vorlaufer, Christoph M Sommer, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” Nature. Springer Nature, 2025. https://doi.org/10.1038/s41586-025-08985-1. ieee: M. Tavakoli et al., “Light-microscopy-based connectomic reconstruction of mammalian brain tissue,” Nature, vol. 642. Springer Nature, pp. 398–410, 2025. ista: Tavakoli M, Lyudchik J, Januszewski M, Vistunou V, Agudelo Duenas N, Vorlaufer J, Sommer CM, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, Jain V, Danzl JG. 2025. Light-microscopy-based connectomic reconstruction of mammalian brain tissue. Nature. 642, 398–410. mla: Tavakoli, Mojtaba, et al. “Light-Microscopy-Based Connectomic Reconstruction of Mammalian Brain Tissue.” Nature, vol. 642, Springer Nature, 2025, pp. 398–410, doi:10.1038/s41586-025-08985-1. short: M. Tavakoli, J. Lyudchik, M. Januszewski, V. Vistunou, N. Agudelo Duenas, J. Vorlaufer, C.M. Sommer, C. Kreuzinger, B. Oliveira, A. Cenameri, G. Novarino, V. Jain, J.G. Danzl, Nature 642 (2025) 398–410. corr_author: '1' date_created: 2025-05-18T22:02:51Z date_published: 2025-06-12T00:00:00Z date_updated: 2026-10-02T09:59:44Z day: '12' ddc: - '570' department: - _id: JoDa - _id: GradSch - _id: Bio - _id: GaNo doi: 10.1038/s41586-025-08985-1 ec_funded: 1 external_id: isi: - '001483477000001' pmid: - '40335689' file: - access_level: open_access checksum: ebc99d7108e728f46db0a009292675ef content_type: application/pdf creator: dernst date_created: 2025-07-03T06:55:20Z date_updated: 2025-07-03T06:55:20Z file_id: '19959' file_name: 2025_Nature_Tavakoli.pdf file_size: 133201290 relation: main_file success: 1 file_date_updated: 2025-07-03T06:55:20Z fulldoi: https://doi.org/10.1038/s41586-025-08985-1 has_accepted_license: '1' intvolume: ' 642' isi: 1 language: - iso: eng month: '06' oa: 1 oa_version: Published Version page: 398-410 pmid: 1 project: - _id: 6285a163-2b32-11ec-9570-8e204ca2dba5 grant_number: '26137' name: Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy - _id: 2564DBCA-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '665385' name: International IST Doctoral Program - _id: 34ba8964-11ca-11ed-8bc3-e15864e7e9a6 grant_number: '101044865' name: Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders - _id: 26AA4EF2-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: W1232-B24 name: Molecular Drug Targets publication: Nature publication_identifier: eissn: - 1476-4687 issn: - 0028-0836 publication_status: published publisher: Springer Nature quality_controlled: '1' related_material: link: - description: News on ISTA website relation: press_release url: https://ista.ac.at/en/news/piecing-together-the-brain-puzzle/ record: - id: '18697' relation: research_data status: public - id: '18677' relation: earlier_version status: public scopus_import: '1' status: public title: Light-microscopy-based connectomic reconstruction of mammalian brain tissue tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd volume: 642 year: '2025' ...